LOCUS       DATROQ010000001        55978 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.001, whole genome shotgun sequence.
ACCESSION   DATROQ010000001 DATROQ010000000
VERSION     DATROQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 55978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55978
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.001"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(8..298)
                     /locus_tag="VBC94_000001"
     CDS             complement(8..298)
                     /locus_tag="VBC94_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEO6978316.1"
                     /translation="MSQIKLTPEELRISAQKYTTGSQSITDVLTVLTQEQAVIDENWD
                     GTAFDSFEAQFNELSPKITQFAQLLEDINQQLLKVADVVEQTDSDIASQINK"
     gene            complement(753..1145)
                     /gene="rpsI"
                     /locus_tag="VBC94_000002"
     CDS             complement(753..1145)
                     /gene="rpsI"
                     /locus_tag="VBC94_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEO6978317.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITINKKDVEEYIPHADL
                     RLVINQPFAVTSTQGSYDVFVNVVGGGYAGQSGAIRHGISRALLEVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1166..1612)
                     /gene="rplM"
                     /locus_tag="VBC94_000003"
     CDS             complement(1166..1612)
                     /gene="rplM"
                     /locus_tag="VBC94_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEO6978318.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKASDKIYYTHSMYPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(1834..1956)
                     /locus_tag="VBC94_000004"
     CDS             complement(1834..1956)
                     /locus_tag="VBC94_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002278327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6978319.1"
                     /translation="MKNLRLKTARASMDLLQQSLAEKVGVSCQTIAAIEKGGYN"
     gene            complement(2493..3353)
                     /locus_tag="VBC94_000005"
     CDS             complement(2493..3353)
                     /locus_tag="VBC94_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6978320.1"
                     /translation="MTFKILTDSTSDLDEKWAQEHNVDIIGLTIELDGKTYETVGDEK
                     ITSDFLLERMQEGAKPTTSQINVGQFEEVFSTYAENDHALLYLALSSHLSGTYQSATI
                     AREMVLDKYPDAQIEIVDTMAASCGEGVLAMLATKERQEGKSLEEVKQKIESLLPKLN
                     TYFLVDDLNHLMRSGRLSKGAAIIGSVAKIKPLLKLDSEGKLVPFAKTRGRKKGIKEI
                     VTQATKTLSYSTLIIAYSGEKDSAQVMKEQLLADERIEEVIIRPLGPVISAHVGSGAL
                     ALFSLGEENR"
     gene            complement(3446..3964)
                     /locus_tag="VBC94_000006"
     CDS             complement(3446..3964)
                     /locus_tag="VBC94_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEO6978321.1"
                     /translation="MKKHSILLVDGYNMIAFWKDTRQLFKSNRLEEAREVLLRKLNHY
                     AHFEHIDIICVFDAQYVPGVRQRYDQYKISVIFTEEDETADSYIERAAAELNQSVLNL
                     VSVATSDLNEQWTIFSQGALRVSARELEQRVATVKSDLDKMSSQIDLSTPKLRPWNDE
                     QLGKLKDFLDGM"
     gene            complement(3961..4716)
                     /gene="rlmB"
                     /locus_tag="VBC94_000007"
     CDS             complement(3961..4716)
                     /gene="rlmB"
                     /locus_tag="VBC94_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEO6978322.1"
                     /translation="MTMKDKQFKEESSDLVYGLHAVTESLRANTGNKLYLQDDLRGKN
                     VDKVKALATEKKVSISWTPKKILSDMTNGGVHQGFVLKVSEFAYADLSEIMTKAENEE
                     NPLILILDGLTDPHNLGSILRTADATNVTGIIIPKHRSVGVTPVVSKTSTGAVEHVPI
                     ARVTNLSQTLDTLKDKDFWIFGTDMNGTPSHKWNTKGKLALVIGNEGKGISHNIKKQV
                     DEMITIPMNGHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(4819..5205)
                     /locus_tag="VBC94_000008"
     CDS             complement(4819..5205)
                     /locus_tag="VBC94_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEO6978323.1"
                     /translation="MIDVRLINGIALAFEGDAVYSLYIRRHLIMQGFTKPNQLHRKAT
                     QYVSANAQALLINAMLEENILTDEEQLIYKRGRNANSHTKAKNADIITYRMSTGFEAL
                     MGYLDMTGQIKRLETLIQWCIETIEK"
     gene            complement(5198..6541)
                     /locus_tag="VBC94_000009"
     CDS             complement(5198..6541)
                     /locus_tag="VBC94_000009"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEO6978324.1"
                     /translation="MIKIYDTMTRSLQDFIPLNEGKVNMYVCGPTVYNYIHIGNARSV
                     VAFDTIRRYFEYCGYQVNYISNFTDVDDKIIKGAAEAGMDTKSFSDKFISAFMEDVAA
                     LGVKPATKNPRVIDYMDEIIDFVKVLVDKEFAYEANGDVYFRVSKSHHYAKLANKTLE
                     DLEIGASGRVDGEGEIKENPLDFALWKSAKSGEVSWESPWGKGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVNVDNEKMSKSLGNF
                     ITVHDMLKSVDGQVIRFFLATQQYRKPVNFTEKAVHDAEVNLKYLKNTFNLPIQENAN
                     DEELEQFVKAFQEAMDDDFNTANGITVIFEMAKWINSGHYTSKVKETLAELLEIFGIV
                     FQEEVLDADIESLIEQRQEARANRDFATADRIRDELAKQGIKLLDTKDGVRWTRD"
     gene            complement(6538..6720)
                     /locus_tag="VBC94_000010"
     CDS             complement(6538..6720)
                     /locus_tag="VBC94_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978325.1"
                     /translation="MKDYVTTQILSILGILAGACLSYLKFAQGNSLLGIIWAAFALMY
                     IVRLYKAYQKQKKEKP"
     gene            complement(6730..7314)
                     /gene="cysE"
                     /locus_tag="VBC94_000011"
     CDS             complement(6730..7314)
                     /gene="cysE"
                     /locus_tag="VBC94_000011"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEO6978326.1"
                     /translation="MGWWKESIAIVKEQDPAARSSLEVILTYPGIKALAAHRLSHFLW
                     NHNFKLLARMHSQFWRFWTQIEIHPGATISEGVFIDHGSGLVIGETAIVEKGAMLYHG
                     VTLGGTGKDKGKRHPTIRKGALISAHSQIIGPIEVGENAKVGAAAVVLADVPADVTVV
                     GVPAKVVRVHGQKDDLQIRSIEHDREESYYSSKL"
     gene            complement(7323..8075)
                     /locus_tag="VBC94_000012"
     CDS             complement(7323..8075)
                     /locus_tag="VBC94_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEO6978327.1"
                     /translation="MTSTNELDIRLRAFINAPDNFLDSIGLVNALHHSTVWASKEPYA
                     IQVDGQEVVPVFTDITDLNHFKEEQESARDMFWESRRSLDVLDEAISHGLAGLVYNLK
                     KEGDFGNSTIFYCEDMVQFMNNYTTILNQLLNEDNIVADIMDKTYLVPAFVHPREEGS
                     FDRFFPTMSTPEGKSYVPVFSNLLSFEKWYNHNDFGGAFRKAQGVILAWTIDDIYKPR
                     NGENEIDDTFGVAINPFDEQQVLVDWSDVEND"
     gene            complement(8077..10206)
                     /gene="pnp"
                     /locus_tag="VBC94_000013"
     CDS             complement(8077..10206)
                     /gene="pnp"
                     /locus_tag="VBC94_000013"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEO6978328.1"
                     /translation="MSKQVFEMIFAGKKLVVETGQVAKQANGSVVVRYGDSTVLTAAV
                     MSKKMSTGDFFPLQVNYEEKMYAAGKFPGGFNKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSFDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVAYVDGNFI
                     INPTAQEQEASALELTVAGTKEAINMVESGAKELSEEIMLEALLKGHEAVCELIAFQE
                     EIVTAIGKEKAEVELLQVDPELQAEIIATHNIALQAAVQVEEKKAREAATEAVKEVVI
                     GEYEARYAEHEEYDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRRVDEIRPLDAVI
                     DFLPQVHGSGLFTRGQTQALSVLTLAPMGEAQIIDGLTPEYKKRFMHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIEGITPQILEEALAQAKKARFEILDVLHGAIAESRPQLAPTAPKIDMIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDEEGNVSIFSSDQAAIDRTKDIIASLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTANVADVLEIGEEVDVKVIKI
                     DDKGRVDASMKALLPRPPKADNPKKES"
     gene            complement(10586..10855)
                     /gene="rpsO"
                     /locus_tag="VBC94_000014"
     CDS             complement(10586..10855)
                     /gene="rpsO"
                     /locus_tag="VBC94_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEO6978329.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNDHIK
                     QHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            complement(10943..12202)
                     /locus_tag="VBC94_000015"
     CDS             complement(10943..12202)
                     /locus_tag="VBC94_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEO6978330.1"
                     /translation="MRAWKGIVLILSSIVVTLVAWQNAGLSEFVVPGLALTSLSLTFL
                     LSTKFRILESYFQGIENMYFYHKVMAVFSMILLLLHKIGLVQGGHGSEFAKTIGSAGL
                     YLFLSIVFVAYFGNFLKYEIWRFIHRFVYLVYILGLVHTFLILGDRILGNTLLSLIVL
                     GYAVIGVISGFYIIFLYSRMRFRRVGYVQKVTHLNHDTTEIEIAMKRPYRYDYGQFTF
                     LKIYQAGFESAAHPFSISGGHDRVIFLTVKASGDYTKSIYKQLKVGTKIALDRAYGHM
                     LFDKDKKEQVWIAGGIGITPFISFIRENSILTKRVDFFYTFSNQDNLIYQDMLESYAK
                     ANPNFKLHLNNSSLQGRLDFSQSVFEGQPTIFMCGPTSMSSTYAKVFRQKDAKSRLVY
                     EGFSFRDSWLSIFLLKTFDKVYSNLIK"
     gene            complement(12311..13240)
                     /locus_tag="VBC94_000016"
     CDS             complement(12311..13240)
                     /locus_tag="VBC94_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEO6978331.1"
                     /translation="MRCSTKEMRLVYRDFLLQANQENKQITVLEADLSSSMSTNVLAS
                     EFGKRYINLGIMEAEMVGLAAGLAIKGYKPYLHTFGPFASRRVFDQVFLSLGYSQLSA
                     TIIGSDAGVSAEMNGGTHMPFEELGLLRLIPKATIFEVSDDIQFEAILKQTLSIDGLK
                     YIRTIRKAPTAVYEGCEDFSKGFIQLRQGKDIALVASGIMVSRAIEAADYLKELGIEA
                     SVIDLFKIKPLPEELKPLLIDQSIVTIENHNRIGGIGSALCEWLSMEKDTTVSRMGID
                     ERFGQVGQMEYLLEEYGLAVKDIVEHCKSIYKS"
     gene            complement(13237..14094)
                     /locus_tag="VBC94_000017"
     CDS             complement(13237..14094)
                     /locus_tag="VBC94_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO6978332.1"
                     /translation="MTSPEERYSSLRRFATEIRLNTLETLNHLGFGHYGGSLSIVETL
                     AILYGDIMDINPEKFKESDRNYMVLSKGHAGPALYSTLYLKGFFDKTFLHSLNTNGTK
                     LPSHPDRNLTPGIDVTTGSLGQGISIATGIAYAQKIENSSYYTYTIVGDGELNEGQCW
                     EAIQFAAHHQLHHLIVFVDDNKKQLDGLTADICNPGDFVAKFEAFGFDAVRVKGDDIE
                     AIDKAIKTFQDSNSVKPKCIVLDSIKGQGVKELEELASNHHLRPDLQQKTMLERAVIS
                     LRESLEVVE"
     gene            complement(14097..15452)
                     /locus_tag="VBC94_000018"
     CDS             complement(14097..15452)
                     /locus_tag="VBC94_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEO6978333.1"
                     /translation="MKGLLDFLVNIARTPAILVALIAIIGLVLQKKGVPDIVKGGIKT
                     FVGFLVVSGGAGIVQNSLNPFGKMFEHAFHLVGVVPNNEAIVAVALTKYGSATALIML
                     AGMIFNILIARFTKFKYIFLTGHHTLYMACMIAVIFAVAGFTSFSLILFGSLALGIIM
                     SVSPAFVQKYMIQLTGNDKVALGHFGSLGYWLSGFIGGIVGDKSKSTEDIKFPKSLSF
                     LRDSTVSITISMAIIYLIVAVFAGEAYIAKEISNGVNGLVYALQLAGQFAAGVFVILA
                     GVRLILGEIVPAFKGISEKLVPNSKPALDCPIVYPYAPNAVLIGFISSFVGGLVSMIV
                     MIVTGTTVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFVQGILISFLPIFLMPVL
                     GGLGFKGSTFSDADFGLTGIILGALNHVGGAIAIVIGIVVILIGLFGISFVGKSTHKE
                     G"
     gene            complement(15465..15749)
                     /locus_tag="VBC94_000019"
     CDS             complement(15465..15749)
                     /locus_tag="VBC94_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6978334.1"
                     /translation="MLRIGTACGSGLGSSFMVQMNIESILKDLGVSDVEVEHYDLGGA
                     DPSAADVWIVGRDLEDSAGHLGDVRILNSIIDMDELRELVTGICQEKGLI"
     gene            complement(15752..17788)
                     /locus_tag="VBC94_000020"
     CDS             complement(15752..17788)
                     /locus_tag="VBC94_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEO6978335.1"
                     /translation="MVDNKTVVIMLVFLARKNLSLYELTVQTKFSIKVIIEQINYLNS
                     FLAKNHLPAIAHSAGRYQLLGDEKEHDKIVSLLEAEQFYLTQEERVCLIYLYSFCRRE
                     FVSNVHYQDFLKVSKNTTLSDIKMLRSKLAKRGISLTYTRAKGYSLVGDEMDKHQVAF
                     QMITQLLESPIGFWSLNYILSSWKFALSYEKLEKTVEYFYESFQLSPIQDRLEKSLYF
                     IILILCRYQRSVDRVLQGSPIVSEQLKELTTIIVTNLSQDISLSKPLDQKEKDYITLI
                     LSGCFEGEGTKDDDFFEALAKAIVDEMETVSLLNFSNKEELLQGLKRHIIPAYFRLKY
                     GLTGDSGYTQNIKEHYSDLFLLVKKALRPLEEQVGLIPDSEISYFVIHFGGYLRQSGD
                     TQSMSYKALILCPNGVSSSLVIKEKLRGLFPQIHFHRVSKIEQLKLIDNQTYDMVFST
                     IFVETKKPNYLVSLMMTAEQVQQLKELVISDFPKACLDDFQLDQLIATIKKYAHVHCE
                     EELKLALRTMVKQDIFRKDVRPLLHQLITEETYQTSSEQMNWKEAIRLAAKPLLASGK
                     ITESYPEAMIEKVEEFGPFINLGKGIAIPHARPEDGVNSVGMSMLVLEQPIYLLDDPK
                     QEIYLLLCIAAIDNETHLKALSHLTTILRDNNNVKALLASRGYQDIEMIIKQED"
     gene            complement(18004..19629)
                     /gene="treC"
                     /locus_tag="VBC94_000021"
     CDS             complement(18004..19629)
                     /gene="treC"
                     /locus_tag="VBC94_000021"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEO6978336.1"
                     /translation="MTIDKRKVVYQIYPKSYKDTTGNGVGDLRGIIEKLPYLAELGID
                     MVWLNPFYPSPQRDNGYDISDYTAINPDFGTMDDFEEMIEVGRQYRIDFMLDMVLNHC
                     SIEHEWFKKALAGDRYYQDFFILRDNPTDWVSKFGGNAWSPFGDTGKYYLHLFDITQA
                     DLNWRNADVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEILENCPINDGKPAYTDRP
                     ITHDYLKMLNNASFGQADSFMTVGEMSSTTIANCILYTAPEREELSMAFNFHHLKVDY
                     KDGQKWTIMAFDFPALRDLFHSWGEGMSEGNGWNALFYNNHDQPRALNRFVDVKRFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDYSSMDDYVDIESLNAYQIMLDEGKSQE
                     EAFSIIRAKSRDNSRVPMQWDDSTNAGFSEGAPWLKVGKSYKEINVAKEKTGLIFTFY
                     QELIRLRKQLPIIADGNYKAAFKDNEKVYAFERHLDKEKLLVLNNFFAEKVKIKLPEN
                     YLQGQVLLSNYKDVTLDETVTLQPYQTLAILVS"
     gene            complement(19851..21881)
                     /gene="treP"
                     /locus_tag="VBC94_000022"
     CDS             complement(19851..21881)
                     /gene="treP"
                     /locus_tag="VBC94_000022"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEO6978337.1"
                     /translation="MEQFKHDAKALLEAIGGKENISAVTHCATRMRFVLNDSSKAKVK
                     VIEELPSVKGTFTNAGQFQVIIGNDVPIFYNAFVAVSGIEGVSKEAAKSAAPKNQNPL
                     QRVLTMLAEIFTPIIPAIIVGGLILGFRNILDAVPFEFLGQKVVDGVRQVDSSGHPIW
                     NTLVDVSTFWSGVDSFLWLPGEAIFHFLPVGIVWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYSVASTSAADIAKNWSWNFGYFTVQKIGYQAQVIPALLAGLSLSYLEIFWRKHI
                     PEVVSMIFVPFLSLVPAIILAHTVLGPIGWTLGKWISAIVLIGLTGPVKWLFGAIFGA
                     LYAPFVITGLHHMTNAIDTQLIADTKTHTTGLWPMIALSNIAQGSAVLAYYFMHRHDE
                     KEAQISLPAAISAYLGVTEPALFGVNVKYIYPFVAGMIGSSVAGLLATTFNVQANSIG
                     VGGLPGFLSINVKYMGYFFICMAVAIFIPLFLTLFFKKSGILTKTEEEKLVSDAVIAS
                     TTETKSAKEKAVVSGTKLSVVSPLSGLAKPLDQASDPVFSQGIMGKGVVIDPSDGELV
                     SPVDATVSVLFPTKHAIGLLTSEGVEFLIHIGMDTVNLEGKGFTSHVAQGDNVKVGDK
                     LITFDIPMIKEEGYIVETPILITNQEEFRPEELIDLPKQIKRGQALMVAKKI"
     gene            complement(22163..22789)
                     /locus_tag="VBC94_000023"
     CDS             complement(22163..22789)
                     /locus_tag="VBC94_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6978338.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEINLEVAPGQVLGIFGQSGCGKTS
                     LSKVLAGFLHPRSGEVLVDGSNLPNKAFRPVQLIQQHPEKTMNPLWPMKKSLEEAYYP
                     SRDLLDAFGIQEKWLNRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIVISHDFAMLEKLCNQCYMIEENRIVSFNGD"
     gene            complement(22773..23576)
                     /locus_tag="VBC94_000024"
     CDS             complement(22773..23576)
                     /locus_tag="VBC94_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6978339.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEIKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNASVTGDMIYRGQSLNSKRIKQLRGKDITLIPQSVN
                     YLDPSMKVKHQVRLGISENSKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDKVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGNGEQLQTEFARSLWRSLPQQEFLKGVTHDLRG"
     gene            complement(23588..24409)
                     /locus_tag="VBC94_000025"
     CDS             complement(23588..24409)
                     /locus_tag="VBC94_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6978340.1"
                     /translation="MILRRRTIVLWQLGIAISLILSILALNLYFHSTPLQTNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVILATVFGVLAGLGNSIID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYHLKN
                     KEFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(24406..25383)
                     /locus_tag="VBC94_000026"
     CDS             complement(24406..25383)
                     /locus_tag="VBC94_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6978341.1"
                     /translation="MKHITTVVIWKILRCLSLVFGVSILTFILLKQSPVDPVMASVNY
                     DTSLTPVQYKAIAHHYGLDKPAPVQYFIWLKNFIQGHLGTSLVYRQPVIDIIRSRAGA
                     SFILMGLSWVLSGLIGFILGTLSAFHQGKLLDRIVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLILPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETEWQIFKNHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(25496..27124)
                     /locus_tag="VBC94_000027"
     CDS             complement(25496..27124)
                     /locus_tag="VBC94_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6978342.1"
                     /translation="MTKYLKYISFVALFLASIFLVACQNQNSQTKERTRKQRPKDELV
                     VSMGAKLPHEFDPKDRYGIHNEGNITHSTLLKRSPELDIKGELAKKYKISKDGLTWSF
                     DLNDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELASKKVKGTRLLDIASNDVRGLSLPYVKK
                     GVVKNSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDRTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGNLKAEFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHYPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSPDLDKANKYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINVGKQGVHSHGHDWSLLTNIAEWTWDESAK"
     gene            complement(27367..28095)
                     /gene="lrgB"
                     /locus_tag="VBC94_000028"
     CDS             complement(27367..28095)
                     /gene="lrgB"
                     /locus_tag="VBC94_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HEO6978343.1"
                     /translation="MELLKTPIFGICFSLILYTIGQHLFKKSKGFFLLQPLFFAMVSG
                     IVILWLMSKGLGTDVKTFYTQAYKPGGDLIFWFLNPATIAFAVPLYKKNDVVKKYWVE
                     ILSSLVIGMIVSLMLIVAISKMVGLSQVGIASMLPQAATTAIALPITAAIGGNTAVTA
                     MACILNAVIIYALGKKLVSFFHLNDSKIGAGLGLGTSGHTVGAAFALELGELQGAMAA
                     IAVVVIGLVVDLVIPIFSHLIGLL"
     gene            complement(28097..28552)
                     /locus_tag="VBC94_000029"
     CDS             complement(28097..28552)
                     /locus_tag="VBC94_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO6978344.1"
                     /translation="MKHTSKETKTKEAPMFIQMSIYAAILLVSQMISMLLPKSLPIPT
                     TVIGLVLMYVLLTAKIIKVEWVDSFGALMISMIGFMFVPSGISVAANLDILKAEGLQL
                     VAVITISTVVMLVVVAYVARLILAIRELSVTDLFKKILPNHYSKKVEEK"
     gene            complement(28722..29462)
                     /locus_tag="VBC94_000030"
     CDS             complement(28722..29462)
                     /locus_tag="VBC94_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEO6978345.1"
                     /translation="MKILILDDEMFARQELSFLVEHSQEVDNPEIFQAEDISEAEKIL
                     FRQQIDLIFLDISLSEENGFTLANQLSQLAHPPLVVFATAYDNYAVKAFESNAVDYIM
                     KPFEQQRVDMALSKVKKLSQLTTASDVEQAIPKKASAELLTLTLSDRSVVVKMQDIVA
                     ASVEDGELTVSTVQKTYTIRKTLNWFKSRAVAPYFLQIHRNTVINLEMIEEIQPWFNH
                     TLLLIMSNGEKFPVGRSYLKDLNEHLTM"
     gene            complement(29443..31188)
                     /locus_tag="VBC94_000031"
     CDS             complement(29443..31188)
                     /locus_tag="VBC94_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEO6978346.1"
                     /translation="MLMVLLFQRLGIIMILAFLLVNNSYFRQLIEERSKRETVVLVII
                     FGLFVIISNITGIEIKGDRSLVERPFLTTISHSDSLANTRTLVITTASLVGGPLVGSI
                     VGFIGGVHRFFQGSFSGSFYIVSSVLVGIVSGKIGDKLKENHLYPSTSQVILISIIAE
                     SIQMLFVGIFTGWELVKMIVIPMMILNSLGSTLFLAILKTYLSNESQLRAVQTRDVLE
                     LTRQTLPYLRQGLTPQSARSVCEIIKRHTNFDAVGLTDRSNVLAHIGVGHDHHIAGQP
                     VKTDLSKSVIFDGEPRIAQDKAAISCPDHNCQLNSAIVVPLKINDKTVCALKMYFAGD
                     KTMSEVEENLVLGLAQIFSGQLAMGITEEQNKLASMAEIKALQAQINPHFFFNAINTI
                     SALIRIDSDKARYALMQLSTFFRTSLQGGQDREVTLEQEKSHVDAYMNVEKLRFPDKY
                     QLSYDISAPEKMKLPPFGLQVLVENAVRHAFKERKTDNHILVQIKPDGHYYCVSVSDN
                     GQGISDTIIDKLGQETVAESKGTGTALVNLNNRLNLLYGSVSCLHFSSDKNGTKVWYR
                     IPNRIREDEHENFNS"
     gene            complement(31215..31859)
                     /locus_tag="VBC94_000032"
     CDS             complement(31215..31859)
                     /locus_tag="VBC94_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6978347.1"
                     /translation="MEKVIIFDMDGVIVDSEYTFLDNKTEMLREEGIDTDVSYQYQYM
                     GTTFEFMWQAMKEEFGLPKTVKEYIAEMNRRRQAIVTRDGVRPIKGAQQLIHWLHQHG
                     YRLAVASSSPMVDIKRNLKELGVTECFEYMVTGEDVSSSKPAPDVFLRAAELLDVDPK
                     VCIVIEDTRNGSLAAKAAGMYCFGFANPDYPPQDLSMADKVISDYQDIYIYLPE"
     gene            complement(31983..32378)
                     /locus_tag="VBC94_000033"
     CDS             complement(31983..32378)
                     /locus_tag="VBC94_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6978348.1"
                     /translation="MYNKVIMIGRLTAKPEMVKTPTDKSVTRATVAVNRRFKGSNGER
                     EADFINVVMWGRLAETLASYGTKGSLISIDGELRTRKYEKDGQTHYITEVLASSFQLL
                     ESRAQRAMRENNVSGDLSDLVLEEEELPF"
     gene            32459..33175
                     /locus_tag="VBC94_000034"
     CDS             32459..33175
                     /locus_tag="VBC94_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO6978349.1"
                     /translation="MTNVTSYKEMLAKPWGKIQYEITFAQLSHIKNQNVLDFGAGFCL
                     TAQHLAKENNVTAIEPNPKLLYDNQSDNIYKILGSYEALRALPDQSFDTIICHNVLEY
                     IDKHNHPAYFDEFSRLLKPNGELSLIKHNIIGKILQSVIFSNDTSTAMELLTGEANFK
                     SASFDQGNIYTLEELKQNTNLLVERYQGIRTFYSLQPNHFKTETDWLNKMLAIELSVA
                     DKAPYKDIAFLQHITLKKSL"
     gene            complement(33229..33855)
                     /locus_tag="VBC94_000035"
     CDS             complement(33229..33855)
                     /locus_tag="VBC94_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEO6978350.1"
                     /translation="MIFTYNREHVGDTLMVIVKDSQGAKLDVDRRGQVARVYLQDSKE
                     TVAWNIFEVSSLIAIEGVGQVTLSDQVIKILNAELLKEGFEDSLVNNIEPTFVVAQIK
                     EMIDHPDSDHLHICQVEINDGKTVQIVCGAPNASVGLKTVAALPGAMMPNGSLIFPGK
                     LRGEDSFGMLCSARELALPNAPQVRGIIELSDQVIVGESFDANKHWKN"
     gene            complement(33888..34211)
                     /locus_tag="VBC94_000036"
     CDS             complement(33888..34211)
                     /locus_tag="VBC94_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEO6978351.1"
                     /translation="MILPESYEEIAAYIDSTKKVVFFFTADWCPDCQFIYPVMPSIEK
                     DFSDFVFVRVNRDDYIELAQQWNIFGIPSFVVVENGQELGRLVNKNRKTKAEITKFLA
                     EINHK"
     gene            complement(34208..34492)
                     /locus_tag="VBC94_000037"
     CDS             complement(34208..34492)
                     /locus_tag="VBC94_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEO6978352.1"
                     /translation="MKNKKILFGTGLAGVGLLAAAGYTLTKKVTDYKRQQITQTLREL
                     FSQMGDIQVFYFNEFESDIKMTSGGLVLEDGRIFEFIYRQGVLDYVEVSK"
     gene            34653..34892
                     /locus_tag="VBC94_000038"
     CDS             34653..34892
                     /locus_tag="VBC94_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978353.1"
                     /translation="MKEKLLVPALNCLLGLLFLGDHWFLFRLQNGELFAIVTLILLCT
                     ASFLINKNHPKNLTLIAMVITFLAVLSFLSTFLLA"
     gene            35077..36144
                     /gene="pepA"
                     /locus_tag="VBC94_000039"
     CDS             35077..36144
                     /gene="pepA"
                     /locus_tag="VBC94_000039"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEO6978354.1"
                     /translation="MSDLFNKIKTVTELDGIAGYEHNIRNFLRQEITPLVDQVETDGL
                     GGIFGVKNTHETNAPKVMVAAHMDEVGFMVSHIQPDGTFRVLEVGGWNPLVVSSQRFT
                     LYTRSGDAIPVISGSVPPHFLRGQSGGTTLPKISDIVFDGGFTDKNEAESFGIAPGDI
                     IVPKSETILTANQKHIMSKAWDNRYGVLMVTELLKSLKDQSLSNTLIAGANVQEEVGL
                     RGAHVSTTKFNPDIFLAVDCSPAGDIYGEQGKIGEGTLIRFYDPGHIMLKDMRDFLLT
                     TAEEAGIKYQYYAAKGGTDAGAAHLKNSGIPSTTIGVCARYIHSHQTLYAMDDFLQAQ
                     AYLQAIVNKLDRSTVDIIKGY"
     gene            36214..36984
                     /locus_tag="VBC94_000040"
     CDS             36214..36984
                     /locus_tag="VBC94_000040"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEO6978355.1"
                     /translation="MKIGIIGVGKMASAIIQGLKQTQHDIVISGSCLERSKEIAERLD
                     VTYAESHQSLINQADIIMLGIKPQLFEKVLLPLDITKPIISMAAGISLARLSQLTRSD
                     LPLIRIMPNINAQILQSCTAICYNNHVSDELRQLAKEITDSFGSSFDIAEINFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKYGFPKEQALPIVGQTVLASSQNLLQGQNSTSDLIDN
                     ICSPGGTTIAGLLDLEKNGLTHSVISAIDATIEKAKKL"
     gene            complement(37005..37670)
                     /locus_tag="VBC94_000041"
     CDS             complement(37005..37670)
                     /locus_tag="VBC94_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6978356.1"
                     /translation="MSFIIKKGKILALLIAFLVINQLVPILAVWLLKNHYQTPFTSIL
                     LIGLELLIIALFLYYAKVKQIIRWKVLLTRKALVTILLGWLSLRVPQIIGYLIMTMQG
                     VKDTANQTVIMELTKQLPLALMLIFAIIGAPIMEEIIFRYIIPKELFAKHQKWGFVIG
                     TLAFALIHSPSDIGSFIIYAGMGAILSFVYYKTEHLEYSIMIHFINNALAYSVLISTM
                     MNN"
     gene            complement(37739..38194)
                     /locus_tag="VBC94_000042"
     CDS             complement(37739..38194)
                     /locus_tag="VBC94_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978357.1"
                     /translation="MLIKGLLSPNSKVSPKDYKVYMRFLSFVFSVCSLIMIVSSFLHT
                     FESNFTKGFSFGSGIALFAGSFYFLVITFSEKRFQHYYTKVFDERNQFIANMSCRLLF
                     LLSVLVLVVLCLINVWFGITFSYAKFLLLILSFVLYGYLLIYFILQKLL"
     gene            complement(38235..38372)
                     /locus_tag="VBC94_000043"
     CDS             complement(38235..38372)
                     /locus_tag="VBC94_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978358.1"
                     /translation="MSRMWTIDFCPPIVYQQYVFISTISISIAMSIFAIYRYASLKKT
                     I"
     gene            complement(38431..38637)
                     /locus_tag="VBC94_000044"
     CDS             complement(38431..38637)
                     /locus_tag="VBC94_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6978359.1"
                     /translation="MKNSLQKLRKSRKLSQAELAVALGVTRQTIISLEKEKYTASLEL
                     AFKIARYFDKQIEEVFIYTESEGG"
     gene            complement(38788..39981)
                     /locus_tag="VBC94_000045"
     CDS             complement(38788..39981)
                     /locus_tag="VBC94_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEO6978360.1"
                     /translation="MSKTIAINAGSSSLKWQLYEMPEEKVVAKGIIERIGLKDSISTV
                     KFDDKKDEQILDIVDHTQAVKILLEDLTKHGIIKDFNEITGVGHRVVAGGEYFKESAL
                     VDDKVVEQVEELSALAPLHNPAAAAGIRAFREILPDITSVCVFDTAFHTTMQPHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHQYVAQEAAKQLGRPLEELKLITAHVGNGVSITANYH
                     GQSIDTSMGFTPLAGPMMGTRSGDIDPAIIPYLVANDPELEDAAAVVNMLNKQSGLLG
                     VSGTSSDMRDIEAGLQSKDPNAVLAYNVFIDRIKKFIGQYLAVLNGADAIIFTAGMGE
                     NAPLMRQDVIAGLSWFGIELDPEKNVFGYFGDITKPDSKVKVLVIPTDEELMIARDVE
                     RLKAK"
     gene            complement(40013..40987)
                     /locus_tag="VBC94_000046"
     CDS             complement(40013..40987)
                     /locus_tag="VBC94_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6978361.1"
                     /translation="MNFEKIETAYELILENIQTIENQLKTHIYDALIEQNSYYLGSSC
                     DLDIVVVNNQKLRQLDLSQEEWRRTFQFIFIKSAQTEQLQANHQFTPDSIGFILLFLL
                     EELTSQETVDVLEIGSGTGNLAQTLLNNSSKELNYMGIEVDDLLIDLSASIAEIIGSS
                     AQFIQEDAVRPQILKESDVIISDLPVGYYPNDDIAKRYAVSSSKEHTYAHHLLMEQSL
                     KYLKKDGIAIFLAPENLLTSPQSDLLKEWLKGYADVIAVLTLPETIFGSRQNAKSIFV
                     LKKQAEQKPETFVYPLTDLQNRENMANFIENFQKWSRENSHYSKNMIE"
     gene            complement(41102..41473)
                     /locus_tag="VBC94_000047"
     CDS             complement(41102..41473)
                     /locus_tag="VBC94_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGG"
                     /protein_id="HEO6978362.1"
                     /translation="MILKKKLKAGILLQAIVLAAVFTLVLQFYLARILATERQYHSQI
                     EASKAYLTAQLAYKTIEGDSISGKCYFTGGYASYLQEGNYLQVKVTLDKGGNYNHKFY
                     RKIDTLQVPLKSNDNKASHKH"
     gene            complement(41451..41843)
                     /locus_tag="VBC94_000048"
     CDS             complement(41451..41843)
                     /locus_tag="VBC94_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEO6978363.1"
                     /translation="MVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLN
                     AEFEGAHLEYLRQNKLYLRKQDKIVTFGKSNKDDFRKTGYNGRGYQPMVYGLDNCQMS
                     QTKSMVKLVFYFKDGLKRTFYYDFKEET"
     gene            complement(41832..41907)
                     /locus_tag="VBC94_000049"
                     /pseudo
     CDS             complement(41832..41907)
                     /locus_tag="VBC94_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793382.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
     gene            complement(41904..42995)
                     /locus_tag="VBC94_000050"
     CDS             complement(41904..42995)
                     /locus_tag="VBC94_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEO6978364.1"
                     /translation="MTLRQVTLKNTHQTSGIDKWISWLKKDISVRNRHKSKKLSLKKQ
                     RKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDRMNKALLEGKDLSKMLGELGFS
                     DTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGL
                     RHYLMPQLGENNFATRLITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTI
                     PLVGSYVKLYLTAYYAREWGNLLSQGVELDQIVKVMQNQKSKLFREIGYDMEEGFLSG
                     KAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQP
                     VIFIFVALIIVMIYAAMLLPMYQNMEILS"
     gene            complement(42880..43812)
                     /gene="tadA"
                     /locus_tag="VBC94_000051"
                     /pseudo
     CDS             complement(42880..43812)
                     /gene="tadA"
                     /locus_tag="VBC94_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            complement(43984..44349)
                     /locus_tag="VBC94_000052"
     CDS             complement(43984..44349)
                     /locus_tag="VBC94_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEO6978365.1"
                     /translation="MYQVVKMFGDWEPWWFIEGWEEDITEIAEYDTLSEALLYFQEEW
                     DRGQEKWPYFQSKSSLLATFWSIKEKRWCEECDEYLQQYHSLMLLKEWQEIPKEESIE
                     RFEVFNKIAELPSACSLNL"
     gene            complement(44463..48113)
                     /gene="rpoC"
                     /locus_tag="VBC94_000053"
     CDS             complement(44463..48113)
                     /gene="rpoC"
                     /locus_tag="VBC94_000053"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEO6978366.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIICDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPMDTPLEPKSLLTEREYREKLQ
                     EYGYGSFVAKMGAEAIQDLLKRVDLDAEIAVLKEELKSATGQKRVKAVRRLDVLDAFK
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDLAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDHDEAVMAYQNGYVHLHTRVGIAVDSMPNKPWTEEQKHKIMVTTVGKI
                     LFNDIMPEDLPYLIEPNNANLTEKTPDKYFLEPGQDIQAVIDNLEINIPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     DINKAFRRGLMTEEDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLTITAITDGKEVTETLEERLIGRYTKKSIKHP
                     ETGEILVGADTLITEDMAAKVVKAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGEVVAIEEDSSTRTKKVFVKGQTGEGEYVVPFTARMKVEVGDEVARGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPLAVNEVEIIEGTPVDA
                     EVTEVSTPTTED"
     gene            complement(48230..51805)
                     /gene="rpoB"
                     /locus_tag="VBC94_000054"
     CDS             complement(48230..51805)
                     /gene="rpoB"
                     /locus_tag="VBC94_000054"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO6978367.1"
                     /translation="MAGHEVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDAG
                     LKEVFEDVLPISNFTDTMDLEFVGYELKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDAKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGDSELVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNLKTRLLNQTIAENLVDGETGEILVEAGTVMTRDVIDSIAEHIDGDLNKF
                     VYTPNDYAVVTEPVILQKFKVVAPTDPDRVVTIVGNSNPEDKVRALTPADILAEMSYF
                     LNLAEGIGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRSTGAVTNEIV
                     WLTADEEDEFTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFPSSIVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAKH
                     DGRVIFSDAEKVEVRREDGSLDVYHVQKFRRSNSGTAYNQRTLVKVGDLVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEDSLRDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGVVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWETVQEAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDVMHVDDLEKARVKQEAEEKQAEQVSEVVQED"
     gene            complement(52329..54626)
                     /locus_tag="VBC94_000055"
     CDS             complement(52329..54626)
                     /locus_tag="VBC94_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6978368.1"
                     /translation="MFKGNKKLNSSKLGDYTPLEFGSIFLRIVKLLSDFIYVIILLFV
                     MLGVGLAVGYLASQVDSVKVPSKNSLVTQVNTLTRVSRLTYSDKSQISEIATDLQRTP
                     VAKDAISDNIKKAIIATEDENFNDHKGVVPKAVLRAAAGSVLGFGESSGGSTLTQQLL
                     KQQILGDDPSFKRKSKEIIYALALERYMDKDSILSDYLNVSPFGRNNKGQNIAGIEEA
                     AQGIFGVSAKDLTIPQAAFLAGLPQSPIVYSPYTADAQLKSDKDLSFGIKRQKNVLYN
                     MYRTRALTKDEYKSYKDYDIKKDFIKPAVATTNHHDYLYYSALSEAQKVMYNYLIKKD
                     NVSEHDLKNDETRATYRHRAIEEIQQGGYTIKTTINKSVYQAMQDAAAQYGGLLDDGT
                     GKVQMGNVLTDNSSGAIIGFIGGRNYSENQNNHAFDTARSPGSSIKPILPYGIAIDQG
                     MLGSGSVLSNYPTTYSSGEKIMHADEEGTAMVNLQESLDISWNIPAFWTYKMLRDRGV
                     DVKNYMEKLDYPIENFGIESLPLGGGIDTSVAQQTNLYQMIANGGVYHKQYMIESIED
                     SNGKVIYNHESKPVRVFSKATATILQQLLHGPINSGKTTTFKNRLQGLNSGLAGVDWI
                     GKTGTTNSTSDVWLMLSTPKVTLGGWAGHDNNASLAKLTGYNNNANYMAHLVNAINNA
                     DGNTFGKSERFRLDDSVIKAKVLKSTGLQPGVVTVNGRRITVGGESTTSYWAKNGPGT
                     MTYRFAIGGTDSDYQKAWSTLGGKR"
     gene            54737..>55978
                     /locus_tag="VBC94_000056"
     CDS             54737..>55978
                     /locus_tag="VBC94_000056"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEO6978369.1"
                     /translation="MNIFDELKERGLVFQTTDEDALRKALEEGSVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDVERSLQTKKTVVSWGNKIR
                     GQLSNFLEFETGDNKAVLVNNYDWFSNISFIDFLRDVGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFYELNKNYNVTLQIGGSDQWGNMTAGTELIRRKSNGVSHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDADAVRFLKIFTFLSLKE
                     IEDIRIQFEEAPHQRLAQKTLAREVVTLVHGEKAYKEAVNITEQLFAGNIKGLSVKEL
                     KQGLRGVPNYHVQTEDNLNIIDLLVTSGVVNSKRQAREDVSNGAIYINGDRIQDLEYT
                     ISENDKLENEITVIRRGKKKYF"
BASE COUNT        17861 a        11381 c         8982 g        17754 t
ORIGIN      
        1 aagccaatta tttattaatt tgtgaggcaa tatctgagtc tgtttgttcg acaacatccg
       61 caactttcaa taattgttga tttatatctt ctaataattg tgcaaattgt gtgatttttg
      121 gagataattc attgaattgg gcttcaaagc tatcaaatgc tgtaccatcc caattttcat
      181 caataacagc ttgttcttga gtcaaaactg ttaacacatc tgtaattgat tgtgatcctg
      241 ttgtatattt ttgtgctgaa atacggagtt cttcaggtgt tagtttaatt tgtgacatca
      301 tttgctccct atttttttaa ctgcagtata gcatatttag atgttcctta cacagataaa
      361 actcataata ggttaatcta ttaaaaatac aaacagcgat ttatattaca tctactttac
      421 tttcaaaacg gaaaggttat caaaagattt taaaaaaaca ccctacaata tcttgcaaag
      481 tgctttgaaa cgttgatata acagtatatt aacattttga gaattgtgat gctttacggg
      541 cttttttaag acctggtttt ctctgacaac tgtaatatat ggtgttatat agtataaact
      601 agctaatact catcaattta catccaaaaa gtgtactatg tattgcaata tattcgcgaa
      661 ggggttatca ggttatcaaa agtaaaattg acaataaaaa aacaccttgc aattgcttgc
      721 aaagtgcttt gaaacgttga tataacagta tattaacgtt ttgaaaattg tgatgcttta
      781 cgtgcttttt taagacctgg ttttttacgt tcaaccatac gtgcgtcacg tgtaagaagt
      841 ccagcacgtt tcaatgaatc gcggaaatct gggtcaactt ctaaaagtgc acgtgagata
      901 ccatgacgga tcgcacctga ttgacctgca tatccaccac ctacaacgtt aacaaaaaca
      961 tcgtatgaac cttgagttga agtaactgcg aaaggttggt tgataacaag acgaaggtct
     1021 gcgtgtggga tgtactcttc tacatctttt ttattgattg taattttacc agtacctgga
     1081 accaaacgaa cgcgtgcaac agcgttttta cggcgaccag tacctgcata ttgtgcttgt
     1141 gccattatat aatgctcctt tcctcttaga taagtcctga gatatcaagt acttctggtt
     1201 gttgtgcagc atgtgtatgc tcaccgccaa caaatacttt caatttcata ccttgtgcac
     1261 gtccaagagt gttatgtgga agcatgcctt taactgattt ttcaatcaaa cgaacagcat
     1321 ttttagaacg aagttcacct gctgagattt gtttcaaacc acctggatac attgagtgag
     1381 tgtagtagat tttatctgat gcttttttac cagttaattt aactttttca gcattgataa
     1441 caatcacaaa gtcacctgta tcagtgtgtg gtgtgaaagt tggtttgttt tttccgcgaa
     1501 gtacgctagc aactactgca gaaagacgtc caagaggtac atctgttgcg tcaacaacgt
     1561 accatttgcg ttcaacttgg cctggtttag ccatgaaagt tgttttgttc atgatttctc
     1621 ctatacgaat tcgtttttgt ttacaggtgt ggatgttccg gtccacgaga gtatttgaaa
     1681 ggttccgggg ccttacaaat ggggtaaaca ataccgctta taatcatatc aaaataaggt
     1741 agcattcgtc aagttatttc ttttatttta tcaattttca tcccaaaata aatcatctac
     1801 agttttattc aaagcttgac acaatttaat cgcttaatta tagcctcctt tttcaatggc
     1861 ggctattgtc tgacatgata caccgacctt ttcagctaga gactgttgta ataaatccat
     1921 actagctcta gctgtcttta agcgtaaatt tttcatatta ctatctgtcc ttatacttaa
     1981 ggtagtatac tgaagttccc atagctatca atgatattcc tataactgct aaaattgcat
     2041 catttcccct tgaaaaataa gtagcaatat caacttccta gtaaaagcat caataaaata
     2101 gttattccta tgagggcaaa tgcgccagca acaaaaacat tttccaaaat ctttcatcaa
     2161 caaacggaga gactcttttt agattagaag tcgtaacaaa atagaaatac cactaaataa
     2221 agttaatatt attataaaat caggtgacac gcttcccttt attagctttc caaaaacact
     2281 ttcaagccat aagataaaaa acatcgtcta aaaacatttt tgataacttt gtaaaaccaa
     2341 ttgttgttgt cgttcatcat attgaggata gttcttattt tatttttcat aatatgtctc
     2401 ctactatttt atagtttcat agcatcatat aaaatacatt ttataaaatg tatactaaaa
     2461 gaacataacg tactgttatg ccctttgatt tgttatcggt tttcttcccc taaagagaat
     2521 agagctaaag cacctgagcc cacatgagct gaaataactg gccctaatgg acgaataata
     2581 acttcttcaa tacgctcatc tgctaataac tgttctttca taacttgagc tgaatctttc
     2641 tctccactat aagcaataat taaagtgcta taagatagtg tttttgttgc ctgagtcaca
     2701 atctctttaa tacctttttt acggcccctt gtcttggcaa atggtactaa tttaccctca
     2761 ctatctaact taagtaatgg tttaatctta gcaacacttc caatgatagc tgcacctttt
     2821 gataagcgac cactacgcat taaatggttg agatcgtcaa ctaaaaagta tgtgtttaat
     2881 ttagggagaa gtgattcaat tttttgtttc acttcttcta aagacttacc ttcttgtcgc
     2941 tctttagttg ctaacatcgc taagacaccc tcaccacatg aagctgccat tgtatccaca
     3001 atctcaattt gagcatcagg atatttgtct aagaccatct cacgcgcaat agttgcactc
     3061 tggtaagtcc ctgataaatg tgaagatagc gctaaatata acaaggcatg gtcattttct
     3121 gcataggttg aaaaaacttc ttcaaactga ccaacgttaa tttggcttgt tgtaggcttt
     3181 gctccctctt gcatacgctc caacaagaag tcactagtaa ttttttcgtc tccgactgtc
     3241 tcataagtct ttccatcaag ttcaatggtt aaacctataa tatcgacatt gtgctcttga
     3301 gcccactttt catctaaatc cgatgtcgaa tccgttagta ttttaaaagt cattgttacc
     3361 tcaatctatt atttttattt gataatcaaa gtagttccaa tattatatca aaaaaattgt
     3421 tggctggcta ataaagtgtt agtttttaca taccatctaa aaaatccttg agttttccta
     3481 gttgttcatc attccacggc cttaactttg gcgtactcaa atcgatttga ctggacatct
     3541 tatccaaatc tgattttact gtcgctacac gctgttccaa ttctctagct gagacacgta
     3601 aagcaccctg agaaaatata gtccattgtt catttaaatc actggtagca acggagacta
     3661 aatttaacac agattgatta agttctgccg ctgctcgttc aatatagcta tccgcagttt
     3721 cgtcctcctc agtaaagata acggatattt tgtattggtc ataacgttga cgtacaccag
     3781 gaacatactg ggcatcaaat acacaaataa tatcaatgtg ttcgaaatgt gcgtaatgat
     3841 ttagctttct aagtaatact tcccttgcct cttctaaacg attgctttta aaaagctgtc
     3901 gagtatcttt ccaaaaagca atcatattgt agccatcaac gagtaaaata gaatgttttt
     3961 tcataggcgg tttctaaata cttcatacat caaaatagca gctgcgacac tggcatttaa
     4021 actctgaaca tgaccattca tgggaattgt aatcatttca tctacttgct ttttgatatt
     4081 atgagaaata ccttttcctt cattaccaat aactagggct aatttacctt tggtattcca
     4141 cttatgcgat ggtgtcccat tcatatcagt gccaaatatc cagaagtcct tatcttttaa
     4201 agtatccaaa gtttgactga gattagttac ccttgcgata ggaacatgtt caacagctcc
     4261 tgtagatgtc tttgaaacca ccggggtcac tccaacagaa cgatgtttgg ggatgataat
     4321 gcctgttaca ttggttgcat cagctgtgcg tagaatagaa cctaggttat gagggtcggt
     4381 cagaccatca aggattaaaa ttagaggatt ttcttcattc tctgcctttg tcattatctc
     4441 tgataaatcc gcataggcaa attccgaaac ttttagaacg aatccttggt gaactccacc
     4501 atttgtcatg tctgataaga tttttttagg agtccaggaa atggatactt ttttttcagt
     4561 cgctagtgct ttgactttat caacgttctt tcctcttaag tcatcttgca agtataattt
     4621 attaccagta ttagctctta aactctctgt cacagcatgt aagccataga caaggtcact
     4681 tgattcttct ttaaattgtt tatctttcat agtcatatta tatcatgaat gctatagcat
     4741 aaagcttttt atagcaatat tttaccaaca aatcctaatg tctctataaa aaagagagga
     4801 tatagcctct ctttttgttt atttttcaat ggtttcaata caccactgta ttagagtttc
     4861 caagcgcttt atctgaccag tcatatccaa gtaccccata agagcctcaa aacctgtaga
     4921 catacgatag gtaataatat cagcattttt agcttttgta tgactattcg cattacgtcc
     4981 tcgtttataa attaactgct cttcatcagt taaaatattt tcttctaaca tggcattaat
     5041 taacaatgct tgggcattag cagaaacata ttgggttgct ttccgatgta actgattagg
     5101 tttggtaaaa ccctgcataa ttaaatggcg tcggatatac aaagaataaa ctgcatctcc
     5161 ttcaaaagct aaagcaatac cattaatcaa acgaacatca atcacgagtc cacctcacgc
     5221 catccttagt atctaacaat ttaattcctt gttttgctaa ttcatctcta atacgatcag
     5281 cagttgcaaa atcacgatta gctcgtgctt cttgacgttg ttctattaaa gattcaatat
     5341 cagcatcaag aacttcctct tggaatacaa taccaaaaat ttctaataat tcagcaaggg
     5401 tttccttaac ttttgaagta tagtgtcctg aattaatcca ttttgccatt tcaaagataa
     5461 cagtaatacc gtttgctgta ttaaaatcat catccatcgc ttcttgaaat gctttcacaa
     5521 attgttctaa ttcttcatca ttagcattct cttggattgg taagttgaaa gtatttttta
     5581 aatatttaag gttaacttcc gcatcatgaa cggccttttc agtgaaattc acgggtttac
     5641 gatactgttg tgtcgctaag aaaaaacgta ttacttgtcc gtcaacgctc ttcaacatat
     5701 cgtgaacagt aataaagtta ccaagagatt tagacatttt ctcgttatca acgttaacaa
     5761 aaccattatg catccagtaa tttgcaaagg tcttacctgt tttagcttca gattgtgcaa
     5821 tctcattcgt gtgatgtgga aattctaaat cagctcctcc accatggata tcaatagtat
     5881 caccaaggat ctcagttgcc ataacagaac attcaatatg ccaccctggg cgccctttcc
     5941 cccaaggact ttcccatgag acttccccag atttagctga tttccaaagt gcaaaatcta
     6001 aaggattctc tttaatctct ccttccccat caacacgacc acttgcacca atctctaaat
     6061 cttccaaagt tttattggct aattttgcat aatgatgtga tttacttact ctaaaataaa
     6121 catctccatt agcttcatag gcaaattcct tatcaactaa aactttaaca aaatcaataa
     6181 tctcatccat atagtcaata actcgaggat tttttgtagc aggtttgaca cctaatgcag
     6241 caacatcttc cataaatgct gaaataaact tatctgaaaa ggattttgta tccatacctg
     6301 cttcagctgc accttttata attttatcat caacatcagt aaagttagaa atataattga
     6361 cttggtaacc acaatactca aaataacgac gaatagtatc aaaagctact acactgcggg
     6421 cattcccgat gtgtatatag ttataaactg ttggcccaca gacgtacatg ttgactttac
     6481 cttcattgag aggtataaaa tcttgtaaac tgcgagtcat agtgtcgtaa atttttatca
     6541 tggtttttcc tttttctgtt tttgataagc tttatacaaa cgaacaatat acattaaagc
     6601 aaaggcagcc caaataattc ctaagagaga gttaccttgt gcaaacttta aatagcttaa
     6661 acaagctcct gctagtatac ctaaaattga taatatttga gttgttacat aatccttcac
     6721 aaatacctcc taaagtttgg acgaataata actttcttct cgatcatgtt caatacttcg
     6781 gatttgaaga tcatcctttt gaccatggac acggacaact ttagctggaa caccaactac
     6841 tgttacgtct gctggcacat ctgcaaggac aactgctgca gcaccgactt tagcattttc
     6901 tccaacctca attggaccga tgatttgaga atgtgctgaa attagcgcac ctttacgtat
     6961 ggttgggtgt cgtttgcctt tgtctttacc tgtgccaccc aatgtaaccc catggtatag
     7021 catagcacct ttctctacaa tggcagtttc tccaatgacc aatcctgaac cgtggtcaat
     7081 aaaaacccct tccgaaatcg tcgctccggg atgaatttca atttgtgtcc agaaacgcca
     7141 aaattgactg tgcatacgtg ctaacaattt aaaattatga ttccataaaa aatgagatag
     7201 cctatgagct gcaagagctt ttattcctgg ataagttaaa ataacttcta gagaactacg
     7261 agcagctgga tcttgttctt tgacaatagc tatactttct ttccaccacc ccatagtttt
     7321 cctcaatcgt tttctacatc agaccaatca actaaaactt gttgttcatc aaatggattg
     7381 atggctactc caaaggtatc atcaatctca ttttctccat tacgcggttt ataaatatcg
     7441 tcaatcgtcc aggctaaaat tactccttgt gctttcctaa aagcaccacc aaaatcatta
     7501 tggttatacc atttttcaaa acttaaaagg tttgagaata ctggaacata actttttcct
     7561 tcgggggtag acattgtagg gaaaaaacgg tcaaaactac cttcttcacg tggatggaca
     7621 aaggcaggta ccaagtaggt tttatccatg atatcggcaa caatgttatc ttcatttaac
     7681 aactgattca agattgtggt gtaattgttc ataaattgca ccatatcttc acagtagaaa
     7741 atagtcgagt tcccaaaatc gccttctttt tttaaattat agaccaatcc tgctaagcca
     7801 tgtgaaatag cttcatcaag aacgtctaaa ctacgacgac tctcccaaaa catatctcga
     7861 gcactttctt gctcctcttt gaaatgatta aggtcagtaa tatccgtaaa aacaggaaca
     7921 acttcttgac catctacttg aatagcatag ggttccttgc ttgcccaaac cgtagagtgg
     7981 tgcaaggcat tcacaagacc tatactatct aaaaaattat ccggtgcatt gataaaagca
     8041 cgcaatctaa tgtctaactc gtttgtactt gtcatattaa gattcctttt taggattgtc
     8101 agcttttgga ggacgtggta ataatgcttt catagaggca tcaactcgcc ctttatcatc
     8161 aattttgatg actttcacat caacttcttc accaatttca agaacatcag ctacatttgc
     8221 tgtacgtgtc catgcaattt ctgaaatatg taccaatgca tctgttttat caaagaggtt
     8281 gacaaaggca ccgaatttct cgatacgaac tactttagca tggtaaactt ctcccacttt
     8341 tgcttcacgt actaaacttg caataatgtc tttcgtacga tcaatagcag cctgatcact
     8401 agagaagata gatacatttc cttcttcatc aatatcaatc ttaacacctg tttcagcgat
     8461 gattttatcg atagtttcgc cgcctttacc gatgacaact ttaatcttat caacatcgat
     8521 ttttatcata tcaatcttag gtgccgttgg tgctaattgt ggacgtgact ctgcgatagc
     8581 tccatgaagg acatcaagaa tttcaaagcg tgctttttta gcttgcgcaa gagcttcttc
     8641 caaaatttga ggggtaatac cttcaatttt aatatccatt tgaagtgctg taatcccttc
     8701 acgtgtaccc gcaaccttaa agtccatatc gccaaaatgg tcttctaatc cttgaatatc
     8761 agtaaggact gtgtagtttg ttccatctga tatgagtccc atggcaatac cagcaaccgg
     8821 agcttttata ggcacaccac ctgccataag agcaagcgtt ccagcacata tagaagcctg
     8881 agatgatgaa ccgtttgatt ctaaaacttc tgcaactagt cggatagcat acggaaactc
     8941 ctccaagctt ggaaggactt gttctaatgc acgctctcct aaagcaccgt gaccaatttc
     9001 acgacgccct gcagctccat aacgtcccgt ctcaccaact gaatattgtg ggaagttata
     9061 gtggtgcata aagcgtttct tatactctgg tgttagccca tcaatgattt gtgcttcacc
     9121 cataggtgct aacgttaata ctgataatgc ttgggtttgt cctcgtgtaa agagacctga
     9181 tccatgaact tgtggtaaaa aatcaatcac tgcatctaat ggacgaatct catccacacg
     9241 acgtccatca ggacgaacct tatcttctgt aatgaggcga cgaacttctg catgttccat
     9301 ttgttctaaa atttcagcaa catcacgcat aatgcgatca tattcttcat gttcagcgta
     9361 acgcgcctca tattcaccta taactacttc tttaactgct tctgtagctg cttctctagc
     9421 ctttttctct tcaacttgta ctgcagcctg taaagctatg ttatgagtag caataatttc
     9481 tgcttgaagt tctggatcaa cttgaagaag ttctacttcg gctttttctt taccaatagc
     9541 agttacaatc tcttcttgaa aggcgattaa ttcacagaca gcctcatgtc ctttaagaag
     9601 agcctctagc atgatttctt ctgaaagttc tttcgctcca gattcaacca tgttaatcgc
     9661 ttctttagta cctgcaaccg ttaattctaa cgcagaagcc tcttgttctt gtgctgtcgg
     9721 attaataata aaattgccat caacataagc aacttgaact ccagcaattg gcccattgaa
     9781 aggaatatcg gaaatcgata atgctaatga tgaaccaaac atagcagcca ttggtgcgct
     9841 agcattttca tcaaatgaaa gaacagtatt gatgacctgg acttcgttac gaaatccctc
     9901 agcaaacatc ggacggattg gtctatcaat aagacgtgct gttaaggtag catccgtact
     9961 tggacgccct tcacgtttgt taaaaccacc cggaaatttc ccagcagcat acattttttc
    10021 ttcgtaattg acttgaagtg ggaaaaaatc acctgttgac attttcttag acataactgc
    10081 tgctgtaagt acagtagaat ctccataacg aacgacaact gatccatttg cttgtttagc
    10141 cacttggcca gtttcaacaa ctaatttttt accagcaaaa atcatttcaa agacttgttt
    10201 tgacatatta ttctccatat tttttgttta cgcgtttcct acaaagttca atctagctgt
    10261 tctaaactca actttgcata aaaccacgta taagctttat tctaacataa ttctataaaa
    10321 ttgagtagcg aaaatccgtg ggggaagctg tattatcatt cattaatgta aacgtaaaaa
    10381 gtctgattat taatactagc aatcagacct tgttacaatg attaaaataa atttctaggg
    10441 ttaatcttag cttgcttaat aaccctcttc ataggttaaa acactttctt actgttggcc
    10501 ccctcaaagt acttacgttc atagtacgtt taaaacaaaa aacactagaa aattttctag
    10561 cgtttttact tgactctgtt gtctattaac gacgaagtcc aagtgattga atcaattcac
    10621 ggtaacggtt aacatctgta cgacgtaggt atgctaataa gttacgacgg tgaccaattt
    10681 ttttcatcaa tccacggtaa gtagcgtggt cttttttgtg ttgtttgatg tggtcattaa
    10741 ggtggttgat ttcccaagta agaactgcta cttgaacttc aactgatcct gtatcacctt
    10801 cgtgacgtgc atattgagca atgatttcat tttttttctc ttttgagatt gccataatat
    10861 attctccttt tattgcttca tccgagtctt aggattggca ctcctaaaac caagaaaaag
    10921 ttggtttgtc ttatagaccc tcttatttta tcaaattaga ataaactttg tcaaaagttt
    10981 ttaatagaaa tatggataac caactgtctc taaaggaaaa tccttcatag actaaacgag
    11041 actttgcatc tttttgccga aagaccttag cataagtaga tgacatacta gttggcccac
    11101 acataaatat cgttggttgt ccttcaaaaa cagactgact aaaatcaagt cttccttgga
    11161 ggctactatt atttaagtga agtttaaaat taggattggc tttagcatag gattctaaca
    11221 tatcttgata aatcaaattg tcttgatttg aaaatgtata aaagaagtca actctcttag
    11281 tcaaaatact attttctcta ataaatgaaa tgaagggagt gatgccgata cctcctgcaa
    11341 tccaaacctg ttctttcttg tctttatcaa ataacatatg accataagct ctatctaagg
    11401 ctatttttgt cccaactttt agctgtttgt aaatggattt cgtataatct cctgaagctt
    11461 taacagttaa aaatatcact ctatcatgac cacccgatat cgagaaagga tgagctgctg
    11521 attcaaagcc ggcttgataa atttttaaaa aagtaaattg tccatagtca tatcgataag
    11581 gacgcttcat tgctatttca atctcagttg tatcatggtt aagatgagta accttttgaa
    11641 cgtaccctac acgacgaaaa cgcattctag aatataggaa aataatataa aatccagata
    11701 taaccccaat gacagcataa cctaatacga ttaaactgag taaagtattc cctagtatcc
    11761 tatctcctaa aatcagaaaa gtatgtacca atcccaaaat atagactaaa taaacaaagc
    11821 gatgaataaa acgccaaatt tcatacttta aaaaatttcc gaaataagct acaaaaacaa
    11881 tacttagaaa aagatataat cccgcactgc caatagtctt tgcaaactca cttccatgtc
    11941 cgccttgtac aagaccaatt ttgtgcaata gaagcaaaat cattgaaaat acagccatta
    12001 ccttgtggta aaaatacata ttttcaatac cttgaaagta actttcaagt atcctaaatt
    12061 tggttgataa gaggaatgtt agcgataaac ttgtcaaagc taatcctggt acaacaaatt
    12121 cacttaatcc tgcattttgc catgctacta gtgtcaccac tatgctagat aaaattaaaa
    12181 caataccttt ccaagctcgc ataacgacca cccctttatt tttagtaagg ataaaatacc
    12241 acaagatact tatacaaaac ttaaaaattt cagaaaataa caaaaaggca actccaatgt
    12301 tgcctttttg ttatgattta taaattgatt tgcaatgctc tactatatct ttaacagcga
    12361 gtccatactc ctctaataaa tattccatct gacctacctg accaaatcgc tcatcaatac
    12421 ccatacgact aacagtagtg tctttctcca tactaagcca ttcgcataat gcacttccga
    12481 taccgccaat acggttatga ttctcaatag ttacaatgga ttgatctatc agtaatggtt
    12541 tcagttcttc tggaaggggc ttaattttaa aaaggtcgat aactgaggcc tcaatcccta
    12601 gttctttgag gtagtcggct gcttctatcg ctctagaaac cataatacca cttgctacaa
    12661 gagcaatatc ttttccctgg cgtaattgta taaaaccttt tgaaaaatct tcgcatccct
    12721 cataaactgc agtgggggcc ttacgaatag ttctaatgta ttttagacca tcaatactta
    12781 atgtttgttt taaaattgct tcaaactgaa tatcatcact aacctcaaaa atagtcgcct
    12841 ttggaattaa ccttagtaga cctaactctt caaaaggcat atgagttccg ccattcattt
    12901 ctgcgctaac tcctgcatct gagccaataa tcgtagctga taattgtgag taacctaacg
    12961 ataaaaatac ttgatcaaag acacgccttg acgcgaaagg accaaaagta tgaaggtatg
    13021 gcttgtaacc tttaatggcg agccctgctg caagacctac catctcagcc tccataatac
    13081 ctagattaat ataacgtttt ccaaattctg atgccagtac atttgttgac attgaactcg
    13141 acaaatcggc ttctaaaacg gtaatttgtt tattttcttg attagcttga aggaggaaat
    13201 cacgatagac aagcctcatt tctttggttg aacatctcat tctaccacct ccaaactttc
    13261 tctcaagctt atcaccgctc gctctaacat tgttttttgt tgtaaatctg gtcgtaaatg
    13321 atgattagaa gctaactcct ccaactcttt cacaccttgt cccttgatac tatctaaaac
    13381 aatgcatttt ggtttgacac tatttgaatc ttgaaaagtt ttaatagctt tgtcaattgc
    13441 ctcaatgtca tctcctttta cacgtactgc atcaaatcca aaagcttcaa atttagcaac
    13501 aaagtctcca ggattacaaa tatcagctgt caaaccatct aattgttttt tattatcatc
    13561 tacaaaaaca attaaatggt gcagttgatg atgcgcagca aattgtatag cctcccaaca
    13621 ttgtccttca tttaattcac catcacctac aatagtataa gtataatagc ttgaattctc
    13681 aatcttttga gcataagcaa ttcccgttgc aatactaatg ccttgaccta atgagcctgt
    13741 cgttacatct ataccaggag ttaaattgcg gtcaggatgc gaaggtagtt tggtaccgtt
    13801 tgtattgagc gaatgaagga atgttttatc aaaaaaacct tttaaataga gtgtactata
    13861 caaggctgga ccagcatgtc cttttgataa gaccatataa ttccgatcac tttctttgaa
    13921 tttttcaggg tttatatcca taatatcacc gtacagaata gccaaagtct caacaattga
    13981 taaactaccc ccatagtgac cgaatcctaa atggtttagc gtttctagtg tatttaaacg
    14041 aatctccgtt gcaaatcgtc ttagagatga atatctttct tctggtgatg tcattattaa
    14101 ccttccttat gggttgattt tccaacaaat gaaatgccaa aaagtccaat taagataact
    14161 acaataccaa taacaatagc tatcgcaccg ccgacatgat taagagcgcc aagaatgata
    14221 ccggtcaaac caaaatcagc atctgagaat gttgaacctt tgaaaccaag tccacctagt
    14281 acaggcatca agaaaatcgg caagaaactg atgaggatac cttgaacaaa tgcaccaata
    14341 gtcgctcctc tcacaccacc agaagcatta ccgataacac ctgccgttgc cccacagaag
    14401 aaatgaggaa cgacccctgg taaaatgaca gtagtgccag tcacaatcat aactatcata
    14461 ctaaccaatc caccaacaaa acttgagata aaaccaatca atactgcatt aggtgcataa
    14521 gggtaaacaa ttggacaatc caaggctggt tttgaatttg gaaccaattt ttctgagatt
    14581 cccttaaagg ctggtacaat ttcacctagg ataaggcgta cacctgctaa aataacgaat
    14641 acacctgctg caaattgccc agctagttgg agggcataaa caagaccatt gacaccgtta
    14701 cttatttctt ttgcaatata agcctcacca gcaaacactg caacgataag gtaaataatt
    14761 gccattgaga tagtaatact tacggtactg tcacgtaaaa atgataaact ctttggaaat
    14821 ttaatatctt ctgttgattt tgatttatct ccaacaattc caccaataaa tccactcaac
    14881 caatagccta aagaaccaaa gtgcccaagt gccaccttat cattacctgt cagttgaatc
    14941 atgtattttt gcacaaatgc aggagatacg ctcataataa tccctaacgc tagacttcca
    15001 aaaaggatga gcgagaacga agtaaagcct gctacggcaa aaataacagc aatcatacaa
    15061 gccatataca atgtatgatg tcctgtcaga aaaatatatt taaattttgt aaaacgagca
    15121 attaaaatat taaaaatcat tcccgctaac ataatcaaag cagttgctga gccatacttc
    15181 gtaagagcta ctgctacaat ggcttcatta ttaggaacta cccccaccaa atgaaaagca
    15241 tgttcaaaca tttttccaaa tggattcaag gaattttgga ctatccctgc acctccagaa
    15301 accactaaga agccaacaaa tgtttttatt ccacctttta caatatcagg aacacctttt
    15361 ttctgcagta ctaaaccgat aatggctatc aaggcgacta aaatagctgg cgttctggca
    15421 atattaacta aaaaatccaa tagacctttc atggcatcaa cctcctagat taagcctttt
    15481 tcttggcaaa tacctgtcac taattctctc aattcatcca tatcaatgat actgtttaaa
    15541 atacgaacat cccccaggtg accagctgaa tcctccaaat cacgaccaac aatccaaaca
    15601 tctgctgcgc ttggatctgc tccacctaaa tcataatgtt caacttcaac atcagaaacc
    15661 cccaaatcct tcaaaataga ctcaatattc atttgtacca taaaacttga acctagacct
    15721 gatccacatg ctgttccaat tcttaacatc tttaatcctc ctgttttata atcatttcaa
    15781 tatcttgata cccacgtgaa gctaacaaag ctttaacatt attattatca cgaagtattg
    15841 ttgtcaaatg cgatagagct ttcaaatgcg tttcattatc aatagctgct atgcataata
    15901 agagataaat ttcctgtttg gggtcatcca ataaataaat aggctgttcc aaaacaagca
    15961 ttgacatacc gacagagttt accccatctt ctggtctggc atgtggaata gcaatacctt
    16021 taccaagatt gataaaagga ccaaactctt cgaccttttc aatcatagct tctgggtaac
    16081 tttccgtaat ttttccagaa gctaacaagg gttttgccgc taaacgaatt gcctctttcc
    16141 aattcatttg ttcagaactt gtctgataag tttcctctgt tataagttgg tgcaacaagg
    16201 gcctcacatc ctttctaaat atatcttgtt ttaccattgt cctcaaagcc aattttaatt
    16261 cctcttcaca atgaacatga gcgtattttt ttatagttgc tattaattga tcaagttgga
    16321 aatcgtcaag gcaagctttg ggaaaatctg aaataaccag ttctttgagc tgttgtactt
    16381 gctctgctgt catcatcagc gaaactagat aattaggttt ttttgtttct acaaatatag
    16441 tagaaaagac catatcgtaa gtttgattgt caatcagttt cagctgctca attttagaga
    16501 cacgatgaaa atgaatttga ggaaataatc cacgcaattt ttccttaatc actaaggatg
    16561 aactaacgcc gtttggacag agtatcaaag ctttataaga cattgattgt gtatcacctg
    16621 attgtctaag atagcctcca aagtgaatca cgaagtaaga aatttcacta tcgggtatta
    16681 atcctacttg ctcttccaaa ggacgtagtg cctttttaac caatagaaat aaatcactgt
    16741 aatgctcttt aatattttgt gtataaccac tatcgccagt taaaccatat ttcaatcgga
    16801 aataagctgg aataatatga cgcttcagac cttgtaataa ctcttcttta ttggaaaaat
    16861 taagaagtga aacagtttcc atctcatcaa cgattgcttt tgccaatgct tcaaagaaat
    16921 catcatcttt agttccctca ccttcaaaac agccagataa gatcaaggta atataatctt
    16981 tttctttctg atctaatggt ttagataagg aaatatcctg acttaaatta gtcacaataa
    17041 tagtcgttaa ctcttttaat tgttcagaaa caataggaga accctgcaaa acacgatcta
    17101 ctgacctttg ataacgacac agtattaaaa tgataaaata taaagatttc tcaagcctat
    17161 cttgtattgg agaaagttga aaagattcat agaaatattc taccgtcttt tctaacttct
    17221 catatgagag agcgaatttc caagaagaca agatataatt caaagaccaa aaaccaatgg
    17281 gagattctag tagttgagtt atcatctgaa aagcaacttg atgcttatcc atctcatctc
    17341 ctactagact atatcccttt gctcttgtat aagtgagtga gatacctctt tttgctaatt
    17401 tagaacgtag catcttgata tctgataaag tagtattctt acttactttc aaaaaatctt
    17461 gataatgcac attggagaca aactctcttc ggcaaaagct ataaaggtaa atcagacata
    17521 ccctttcttc ctgagttagg taaaattgct cagcttctaa taaggagaca attttatcat
    17581 gctctttttc atctcctaat aactgataac gtccagcaga gtgagcaata gctggtaggt
    17641 gattttttgc taaaaaacta ttgagatagt taatttgctc tatgataact tttattgaaa
    17701 actttgtttg cactgttaac tcatacaagg aaagattttt ccgagctaaa aataccaaca
    17761 ttatcacaac agtcttatta tcaaccattt gttttccttt ctatttttat tttatagtaa
    17821 ccgtttacat ttttctaggt catttgagcc caatctggca ctactatcaa ttgaatgaat
    17881 ttgggctatt gttatagagt aaagaaaaaa gaaagaaatt gcaaaatcta gagcaagatt
    17941 ccttcccaca agcagcactt aaaaaacctt tacttaattg aataagtaaa ggttttttaa
    18001 atgttatgac actaagatag ctaaagtttg ataaggttgc aaagtaaccg tttcatcaag
    18061 agtaacatcc ttataatttg atagaagaac ttgtccttgg agataattct ccggtaattt
    18121 tatcttaact ttctcagcaa agaaattatt taaaacaaga agtttttcct tatcaagatg
    18181 gcgttcaaaa gcatatactt tttcgttatc tttgaaagct gctttataat tcccgtcagc
    18241 tattattggc agttgtttac gtagccttat caactcttga tagaaagtaa atatcagccc
    18301 tgttttctcc tttgcgacat tgatttcttt atatgatttt cctactttca accatggagc
    18361 gccttcagaa aaacctgcat tcgtactatc atcccattgc attggtactc gagaattgtc
    18421 tcgagatttc gctcgaataa tgctaaatgc ctcttcttgt gacttgcctt catcaagcat
    18481 tatttggtag gcattcaaac tctcaatatc cacataatca tccatagaac tgtaatcggg
    18541 atcaagcatt ccgatttctt cacccatata tatataagga gtaccacgcg ataagtggat
    18601 agatgctgct aacatggttg ctccttcatt tcgaaaacgt ttcacgtcta caaaacgatt
    18661 taatgctcgc ggttggtcat gattattata gaataaagca ttccaaccat ttccttcgct
    18721 cattccttcg ccccaactat ggaaaaggtc acgtaatgca ggaaaatcaa aagccataat
    18781 ggtccacttt tgcccatctt tataatctac tttgaggtgg tgaaaattaa aagccatgga
    18841 taactcttca cgttctggtg cagtgtaaag aatgcaattt gcaattgtcg ttgaagacat
    18901 ttcaccaact gtcatgaagg aatcagcttg tccaaaacta gcattattca acatcttgag
    18961 ataatcatgt gttattggac gatcagtata agctggtttt ccatcattaa ttgggcagtt
    19021 ctcaagtatc tcatctttcc caattaaatt aatcacatca aatcgaaaac ctttaacccc
    19081 tttatcacgc caaaaattga caactttaaa aagttccttg cgaacatcgg cattgcgcca
    19141 attaaggtca gcttgagtga tatcaaaaag atgaagataa tacttccctg tatcaccaaa
    19201 tggtgaccaa gcatttccac caaatttaga tacccaatct gttggattat cacgtaagat
    19261 aaagaaatct tgatagtagc gatctcctgc aagtgctttt ttaaaccact catgttctat
    19321 tgaacaatga ttaagcacca tatcaagcat aaaatctata cggtattgac gtcccacctc
    19381 aatcatttct tcaaaatcgt ccatcgttcc aaaatcaggg ttaatggctg tataatcaga
    19441 gatatcatat ccattatctc gttgtggact tggataaaat gggttgagcc aaaccatatc
    19501 aataccaagt tctgctaaat agggtaattt ttcgataata ccacgaagat caccaacccc
    19561 attaccagtt gtgtctttgt atgattttgg ataaatttga tagacaactt tacgtttatc
    19621 aatcgtcata ttctgctcct ttcccaaaat gctgctgtat ttagcagtct aactataaca
    19681 tcaactatca tcaaaaatat aacattttta cagcgatatt tgttcccatg agttttttct
    19741 tatgttcaaa ataattcaag gagctaatgt attaagcccc tcaaacaatt cctcaaatta
    19801 cctctcattt tataagagtg ctctttttta aaaaggatac ctaattactt ttaaattttc
    19861 ttggcaacca ttaatgcttg tccacgtttt atctgtttgg gtaaatctat caattcttct
    19921 ggtcggaatt cttcttgatt agtaatcaga ataggagttt ccacaatgta tccttcttct
    19981 tttatcattg gaatatcgaa agtaatgagt ttatctccta cttttacatt gtcaccttgg
    20041 gcaacatgag aggtaaaccc ttttccttct aaattaacag tatccatacc aatatgaata
    20101 agaaactcaa caccctctga tgttaataat ccaatagcat gtttcgtagg gaaaagaact
    20161 gaaactgtcg catcgaccgg tgaaactagt tctccatcac ttggatcgat aaccactccc
    20221 ttccccataa ttccttgtga aaagacagga tcactagctt gatccagtgg tttagctaaa
    20281 cctgataaag ggcttactac actaagcttt gtacctgaga cgacagcttt ttcttttgct
    20341 gattttgttt ctgttgttga agcaatgaca gcatcagata ctaatttttc ctcttcagtt
    20401 tttgtcaaaa tacctgattt tttaaagaat agtgtcaaaa ataaaggaat aaagatagcc
    20461 actgccatac agataaagaa atatcccata tatttaacat taattgacag gaagcctgga
    20521 agaccgccta caccaataga gttagcttgg acattaaaag ttgtcgccaa aagacctgcc
    20581 acacttgaac caatcatacc agcaacaaat ggataaatgt atttaacgtt tacaccaaaa
    20641 agcgccggct ctgtaacacc aaggtaggct gagatagcag ctggaagtga tatttgagcc
    20701 tctttttcat catgacgatg cataaaataa taagctagta cagctgaacc ctgagcaata
    20761 tttgaaagag caatcatcgg ccaaaggcca gttgtatgtg tttttgtatc tgctatgagt
    20821 tgagtatcga tagcatttgt catgtggtgc aaaccagtaa taacaaatgg ggcatacagg
    20881 gcaccaaaaa tagcaccaaa gagccatttt acaggaccag tcaatccaat aagaacgata
    20941 gcagaaatcc atttacctag tgtccaacca ataggtccaa ggacagtatg agccaagata
    21001 atagctggaa ctaaggatag aaatggcaca aaaatcattg aaacaacttc aggaatatgt
    21061 tttctccaga agatttctaa gtaagacagg ctcaaaccag ctaacagagc tggaataact
    21121 tgagcttgat aaccaatttt ttgtactgta aagtaaccaa aattccaaga ccaattttta
    21181 gcaatgtctg ctgctgaagt actagctaca gaataagcat taagtaattg tggcgacact
    21241 agacagatac ctagaacaat accgaggatt tgagtagtcc ccatcttacg agtcacagac
    21301 catacaatac caactggtaa gaaatgaaat atagcttcac ctggtaacca taaaaaagag
    21361 tctactccgc tccaaaacgt tgaaacatct actagagtat tccaaattgg atgcccagaa
    21421 gagtctactt gcctgacacc atcaacaact ttttgaccta aaaattcaaa tggaacggca
    21481 tctaaaatat tacgaaaacc aaggatcaga ccaccaacaa taatggctgg aataatcggt
    21541 gtaaatattt cagccagcat tgtcaacaca cgctgtaaag ggttttgatt ttttggggca
    21601 gccgattttg ccgcctcttt agacacaccc tcaataccag aaacggctac aaaagcatta
    21661 taaaaaatag ggacatcatt accaataatg acttgaaatt gcccagcatt ggtaaaggta
    21721 cccttgactg atggtaactc ctcaattacc tttaccttag ctttactact gtcattcaaa
    21781 acaaatctca tgcgtgtggc acaatgtgtc actgcactaa tattttcttt acccccaatt
    21841 gcctctaaca aggctttggc atcatgttta aattgttcca tctgtttatc cttatctatt
    21901 taacggagag tctgtgactc caaggcagaa acttaaagaa gactagctta caatataatt
    21961 gtaaacgatt acatatccct atgcaaatcc ttagcttatc tttttaaaaa atctataaat
    22021 tataaaataa ttagtcaata atacccaaaa cattagaatt tctcaccaac gttaccaaaa
    22081 tgttagagtc atgagtaggt cagtcacccc caatactctc ttaataaaaa acacctacta
    22141 attgatatag taggtgagtt ttttaatctc cattgaaaga tacgatacga ttctcttcaa
    22201 tcatatagca ttggttacaa agtttctcta acatagcgaa atcatgacta ataacgatga
    22261 gacctaaatt tctatccttt acaatctcca acaggctttt ccatacacta gcttgtgtaa
    22321 tactatccaa catagtagtc atttcatctg caataaggta tttagtctct ggatgtaatg
    22381 aacgcacaat cgaaaagcgt tgtaattccc ctcccgagag ttcactaggg cgacgattta
    22441 gccatttttc ttgaattcca aaagcatcta gtaaatctcg acttgggtaa taagcttctt
    22501 ctaaactttt tttcataggc cataaaggat tcatcgtttt ttcaggatgt tgttgaataa
    22561 gttgtacagg tctaaatgct ttgttaggca aattactgcc atcaactaat acttcaccag
    22621 atctaggatg taaaaaacct gcaagcactt tggataaact agtttttcca caaccacttt
    22681 gtccaaatat acctaaaact tgaccaggtg ctacctctaa attaatctcc ttaaaaagcc
    22741 attgatcttt tttatgataa aagccaagct ttttagcctc taaggtcatg agtaactcct
    22801 ttcaaaaatt cttgctgtgg gagagagcgc cataaacttc tagcaaattc tgtttgtaac
    22861 tgctctccat ttccgctaaa gaaactagct ggagctgttt caatagcttt tccctcttta
    22921 aaaatagtaa tacgatcagc aatttgacta gctgctacaa tatcatgagt gataaatata
    22981 acgcttattc ctttatctgc aaaggagcgt agttggtcta aaaccatttg cagagcatct
    23041 ggatgtaatc caggggtggg ctcatccgca ataatcaaag aaaccttatc actaatacac
    23101 gttgtaaaca aaacacgtcg gagcattccg ccagaaagtt ggaaaggata caagtcacca
    23161 tcactttctt ttaaaccaaa ctgttgaaac aatccttctt gagtagcctt tgaattttct
    23221 gagataccta agcgcacctg atgtttgact ttcatagatg gatctaaata attaactgat
    23281 tgtggaatca acgtaatatc ttttcctcgc aactgtttaa tgcgtttaga atttagtgat
    23341 tgaccacgat aaatcatatc tcctgttaca gatgcatttt taggaagaat atccataata
    23401 gcatgtgcta ataaactctt ccccgaacca ctagcaccta taatagctaa taactcacct
    23461 tttttaattt ctaaattcag cgcttgtatc ggtgttgatt gaaatggttt taaaaatctt
    23521 ccgtattgag tgaaggtgat ggagaggtct ttaatgctta ataatgtttc tgtcatatct
    23581 ctacctccta aaaatgatca gtttgagggt aaaagagttt ctttaaagat tctccgatag
    23641 tatcaaatgc attgacaacc aaaataagat aaagtcctgg aaagataacc aaccaccaat
    23701 ttccaagaga gatatgctta gctgcctctg acagaatgat accaaccgaa ggttgttcgg
    23761 cagagagccc aaatcctaag aaagtcattg atgcttcatg taggatgaca tgtggaaata
    23821 ataggataaa accaatgaaa atttgagaag caatcaaagg caggatatga tgcctcacaa
    23881 tataataagg cgtttttccc atacttttag aaagttggac aaattcttta ttctttagat
    23941 gatagacttc attgcggata agccttgcta aagaaggcca atgtgtaaca gccgttgcaa
    24001 tgatgacccc ttgagcacct ttcccaacaa caaaagaaat gagaatcata aaaatcaaat
    24061 gaggcatacc aataaacaaa tcaactaacc atgctattat tttatcaata atgctatttc
    24121 ctaaacctgc aagcactcca aaaactgtcg ccagaatgac ccccataagg gcacctaata
    24181 agccgacttg tagagagaaa taaagtcctt taatcgttct gacaaacata tccctaccta
    24241 aaccatctgt cccaaaaaga tggtttaatg aaggagcaag gttccgtaaa gctgcattgg
    24301 tttgcaaggg agtactatgg aaataaagat ttaaggctag aatactaaga atgagagaaa
    24361 tggcgatacc cagttgccat aaaacaatag ttcgacgtct caatatcata cttttctcct
    24421 taactgtgga ttgattatgc tatttaaaat atccgcaata agattgcccg caaaaacaaa
    24481 taatgtccct atcatcacaa tagctagaag tagcggtgta tcacttttaa gtcctgcttc
    24541 agttagggta gaccctagtc ctggatatga gaaaacttgc tcagcaagaa cggatcctcc
    24601 aaacaattct ccaaaatagg aaaaatgcag tgtaatagct ggtacgatag catttctaag
    24661 acaatgattt ttaaaaattt gccattccgt ttccccacgc gctctggcaa ataagacata
    24721 ttcactagaa agtaccgaca tcattttagt tctagtatga agagttacat tggcaatgcc
    24781 tagaatactt agcgtgaaaa caggtaaaat aaggtgctta atacgatcag ctaacgtaat
    24841 atcttgactc aaagtaccta tcggagaaga aataccaatc gggaaccacc ccagctggac
    24901 agaaaagatt aataaaaaaa tgagcccaat ccaaaaagtt ggtactgata tctgaaggta
    24961 agaaaaccac cttacaattc gatcaagcaa tttcccttga tggaaagctg ataaagttcc
    25021 taagataaat ccaataagac ccgataagac ccaggagagt cccataagta tgaaagaggc
    25081 acctgcccgt gatctaataa tatcaataac aggttgccga taaactagtg aagtccctaa
    25141 atgtccctgt ataaaatttt ttaaccaaat aaaatactgg accggagctg gcttatccaa
    25201 gccatagtgg tgagcaatcg ctttgtactg aacaggggtt agtgacgtgt cataattgac
    25261 acttgccatg actggatcaa ccggtgattg ttttagtaaa ataaaggtca aaattgatac
    25321 tccaaaaacg agtgacaaac acctaagtat cttccagata actacagtgg taatatgttt
    25381 cacagataaa tctccttaat tgttaaaaaa ggagtcgagg aaaccattca caccataagg
    25441 tgtacattgt tttctctgac tccaaactta aagtagccta cattaactaa attagttact
    25501 tagcagattc atcccaagtc cattcagcaa tgttagtcaa taatgaccaa tcatgaccat
    25561 gactatggac accttgttta cctacattga tacgtttatc accaatataa gtatggttaa
    25621 gactcaccaa ccatacattt ggtaaatctc caagagtgga agcacctgtt ttgccatccc
    25681 actgagctaa cttccaatat ttgttagctt tatcaaggtc gggagatgtc attgctttgt
    25741 caaggtactt agtcacagta ggattgttat aaaaagtaat attggtccaa cctttaccag
    25801 ctaaacttgg gtaatgtgat tcataaaatt gctgcgcatg atgacgtcct cccgcataaa
    25861 gtaaggctga atcatgtgac ttagttgcca tttcatccca gttactagcc ttgagtttaa
    25921 tagtaatacc taaggcttta gcttgctccg ctacttcaac ggctaagttt gctcgtaatt
    25981 gatcattagt agggtagtaa aggtcaaatt ccgctttaag atttcctttt ttgcggctac
    26041 cgtctgcttg ttctttccat ccagcttttg tcaaaagttg cttagcttta gctactttat
    26101 tatctttaat tgctgttttt ggattccaga atggtgttct atcaataatt gaataagctg
    26161 gtttaccata accatttaaa acagtatcca gaactttttg tctatttaaa ccaatagtca
    26221 aggcttttct gattgctgga tcactagtga catcatttcc tactggataa ccgtctggtg
    26281 aatttttcac aacacctttc ttcacataag gtagcgataa cccacgaaca tcatttgaag
    26341 caatatctag aagacgtgtt cctttgactt tcttgctagc aagctctggc gttgcgtaaa
    26401 tcatgtcaac atcaccagat tctaaagcag ctagtgctgt attttcatca agtaataccc
    26461 aagtccactt tttaaaataa ggctttttac cgtgccaata tggatttcga acgaagattg
    26521 cttgttctcc agccttatat tcttttacca tgtaaggtcc tgaaccgata ggattgctct
    26581 tatacttatc attgtaatgt tttttaggga cgattgggat ttctgtcaac tgtgctgtaa
    26641 atgtcgaatg cgcctcagtc aaatgaatat ttacctggtt tttcccaact acttcaacat
    26701 tcttaataaa ggtcaaatcc caagcttttc catctgcttt caacatatca taagtaaact
    26761 taacatcgtc agcagtaaca ggctcaccat tggagaattt aaaatcatca tttaagtcga
    26821 acgaccacgt taagccatcc ttagagattt tatatttttt agcaagctct ccttttatat
    26881 ctagttcagg agaacgtttc aatagggtac tatgagtaat attaccttca ttatggattc
    26941 cataacggtc ctttggatcg aattcatgag gaagctttgc ccccatagag acaaccaatt
    27001 catctttggg tcgttgtttt cgtgttcgct cctttgtttg tgaattttga ttttgacaag
    27061 ctactaaaaa gatactcgcc agaaacaaag caacaaaaga gatgtacttc aaatatttag
    27121 tcactattaa acctcctttt ttaattgata aataatattt ttgacaactt ttttcattat
    27181 aacaagaaaa aagtaggaga acaattactt aactggttaa ttagaaaatt tgttatttta
    27241 tcattaataa cttaaagtta ggtaaaacac aattctagac tctagctcat aaaccatgtt
    27301 aatccaatgt attttaatag acatcataaa aaggctgaat aaattcagcc ttaaccacgc
    27361 tattagttat agcaaaccaa tcaaatgact aaagatagga ataaccaaat caactaccaa
    27421 accgataacc accaccgcta tagctgccat cgcaccttgc agttctccca attccaatgc
    27481 aaaggctgct ccgactgtat gaccagaggt ccctagacct aatcctgcac caatcttact
    27541 atcattcaaa tggaaaaatg acactaattt tttacctaat gcataaataa taactgcatt
    27601 taagatacac gccattgctg tcacagctgt gttccctcca attgctgctg ttattggaag
    27661 agcaattgct gttgttgctg cttgtggcaa cattgaagca attccgactt gactaagccc
    27721 aaccatttta gaaatagcga cgataagcat aagggaaaca atcataccga ttactaaact
    27781 gctgagaatt tctacccaat attttttaac aacgtcattt ttcttataga gaggaactgc
    27841 aaaagcaatt gttgctggat ttaaaaacca aaatattaaa tcgccacctg gtttataagc
    27901 ttgtgtataa aatgtcttaa catcggttcc taaacctttt gacataagcc aaagaatgac
    27961 aataccacta accattgcaa agaaaagagg ctgcaaaagg aagaaacctt tactcttctt
    28021 aaataaatgt tgtcctatcg tatagagtat taaagaaaag caaataccaa agatgggtgt
    28081 ttttaagagt tccatattat ttttcctcta ctttcttact atagtggtta ggtaatatct
    28141 ttttaaataa atctgtgaca ctgagttctc tgatagcaag tattaatcgt gcaacgtatg
    28201 ctaccactac taacataaca acagttgaga ttgtaataac agcaactagt tgcaagcctt
    28261 cagcttttag gatatcaagg ttcgctgcta ctgatattcc tgaggggaca aacataaaac
    28321 caatcatgct tatcatcaaa gccccaaaag agtcaaccca ctctaccttg ataattttag
    28381 cagttagcaa tacgtacatt agcaccaaac caataaccgt cgttgggatt ggtagtgatt
    28441 tcggtaataa cattgaaatc atttgtgaaa ctaatagaat agctgcatat attgacattt
    28501 gaataaacat tggagcttct tttgttttgg tttctttact ggtgtgtttc atagtcacta
    28561 ccccttcctt ttattacaat gttttaactt gttttattga tagctatatt gtacttcaaa
    28621 ataaataaaa cgttttcatt ttcagcctca gttaccaaaa aaagaagccg aattgcaaat
    28681 tcaagcttct aaacaacaaa gggaagttcg caaaagcact tttacatggt taaatgctca
    28741 tttaggtctt ttaaatacga tcgacctact ggaaatttct ctccattaga cataattagt
    28801 aacaaagtat gattaaacca aggttgaatt tcctcaatca tctctagatt aatcactgtg
    28861 tttcgatgaa tctggagaaa atatggtgct acggcgcgtg acttaaacca attgagtgtt
    28921 ttccgaatag tgtaagtttt ctgaactgta ctaacagtta attcgccatc ttcaacacta
    28981 gctgcaacga tatcttgcat tttaacaaca acactgcgat ctgataatgt caaggttaac
    29041 aattcagcac tggctttttt aggtattgct tgctctacat ctgaagcagt ggtgagttgg
    29101 ctcaatttct tcacttttga tagagccata tcaacgcgct gttgttcaaa cggtttcatg
    29161 atataatcaa cagcattact ttcaaaagct ttcacagcgt agttatcata agctgtcgca
    29221 aaaacaacaa gtgggggatg tgcaagttgg ctcaactgat tagccaaagt aaagccattt
    29281 tcttcactaa gcgaaatatc taaaaaaatt aaatcaattt gctgtctaaa taagattttt
    29341 tcggcttcac tgatatcctc agcctgaaaa atctcagggt tgtcaacctc ctggctatgt
    29401 tcaactaaaa acgataattc ttgtctggca aacatttcat catcaagaat taaaattttc
    29461 atgctcatcc tcccttattc tattaggtat tcgataccaa acttttgtac cattcttgtc
    29521 gctcgaaaaa tgaaggcaac ttacactacc atataataaa ttcagcctgt tatttagatt
    29581 aactagagca gtacctgtcc ccttactctc tgcaactgtt tcttgaccta atttatcaat
    29641 gatagtatct gagattcctt gtccattgtc actaacagaa acacaataat aatgaccatc
    29701 tggctttatt tgaaccaata tatggttgtc cgtcttacgt tctttgaaag catgtcgaac
    29761 tgcattctct accagtacct gtaaaccaaa aggcggtaac ttcatttttt ctggtgcact
    29821 aatatcataa gataactgat atttatcagg gaaacgtaat ttttcaacat tcatataagc
    29881 atccacatgt gatttttctt gctcaagcgt tacctcacga tcctgaccac cttgcaaact
    29941 tgttctaaaa aaagtactta actgcatcag tgcataacgt gctttatcag aatcaatacg
    30001 gattaatgca ctaattgtgt taatggcatt aaagaagaaa tgagggttga tttgtgcttg
    30061 taaagccttt atctctgcca tactggctaa cttattttgt tcctctgtta tccccattgc
    30121 cagttgtcct gaaaatattt gcgctaaacc aaggactagg ttttcctcca cctcagacat
    30181 tgtcttatct cctgcaaagt acatttttaa ggcacacaca gttttatcat ttatttttag
    30241 aggaactaca atagcagaat ttaactgaca gttgtgatct ggacaagaaa tcgccgcttt
    30301 atcttgcgca attcttggtt cgccatcaaa aataacactt ttagataagt ctgttttgac
    30361 cggttgtcct gcaatatggt gatcatggcc aacaccaata tgagctaata cgtttgaccg
    30421 atctgttaat cccacagcat caaagttagt atgcctcttt ataatttcgc aaacgctcct
    30481 agcagattgc ggtgtcaaac cttgtctaag gtagggcaga gtctgtcgag tcaattcaag
    30541 aacatctctc gtttgaactg cgcgtaactg actttcattt gacaaataag ttttcaaaat
    30601 cgcaaggaaa agtgtggaac ctaaactatt taaaatcatc attggaatga caatcatttt
    30661 gacaagttcc catcctgtaa aaatgccaac aaatagcatc tggatacttt cggcaataat
    30721 actaattaaa ataacttggc ttgttgaagg gtagagatgg ttttccttaa gcttatcacc
    30781 aatctttccg ctaacaatgc cgactagaac tgaactgaca atatagaaag aacctgaaaa
    30841 gcttccttga aaaaagcgat gaactcctcc aataaaacca acaattgatc caaccagagg
    30901 tccaccaacc aaacttgccg ttgtaataac taaagtcctt gtattagcaa gtgagtcaga
    30961 atgggaaatc gttgttagaa aagggcgctc gaccaaactt cgatcccctt ttatttcaat
    31021 tcctgttata ttagatataa taacaaacaa gccgaaaatg atgacaagga ctaccgtttc
    31081 acgtttagac cgctcttcaa ttaactgtct gaaataacta ttatttacca ataaaaaggc
    31141 taaaatcata ataattccta gcctttggaa taacaacacc atcaacatat gcttatcctc
    31201 ctcaagatac cattttactc cggaagataa atatagatat cttgataatc tgaaataacc
    31261 ttgtctgcca ttgataaatc ttgcggtgga taatcaggat tggcaaagcc aaagcaatac
    31321 attcctgctg ccttagcagc taaactccca tttcttgtat cttctataac tatacaaact
    31381 ttagggtcta catctaaaag ctctgcagct ctaagaaaaa catctggtgc tggtttagaa
    31441 gacgatacat cttctcctgt aaccatgtat tcaaagcatt ctgtcacacc caactctttt
    31501 agattacgct taatatctac cattggagat gaggacgcaa ctgctaagcg atagccatgt
    31561 tggtgtaacc aatgaatcag ttgctgagct cctttaatgg gcctaacacc atcacgcgtc
    31621 acaatcgctt gtcgacgtct gttcatctca gcaatatact cttttactgt tttcggtaag
    31681 ccaaactcct ctttcatagc ttgccacatg aattcaaaag tagtccccat gtactggtat
    31741 tgatacgaga cgtccgtatc aataccctcc tcacgtaaca tttctgtctt attatctaaa
    31801 aacgtgtact cggaatcaac aataacgccg tccatatcaa aaataatgac tttttccatc
    31861 ttaaacctgc tttctatccc ctttattgta atcgtttgcc tttctatttt caataaaaaa
    31921 aggttgcttt ctgtggaaag caaccagtat atttagaaat gatattttga gagtgatttt
    31981 atttaaaagg ggagctcctc ttcttccaat actaaatctg acaaatcacc agaaacatta
    32041 ttttcacgca tagcacgttg ggcacggctt tctaacaact gaaatgatga tgctaatact
    32101 tcagtgatat agtgcgtttg accatccttt tcgtacttgc gcgtacgcaa ttcaccatct
    32161 attgaaatta aagagccctt tgtcccatag ctcgcaaggg tttccgctag acgaccccac
    32221 ataaccacat taataaaatc tgcttcacgc tcaccattac ttcctttaaa gcgtctatta
    32281 acagcaacag ttgcacgcgt tactgacttg tcagttggtg tttttaccat ctcaggcttt
    32341 gctgttagac gcccaatcat aataacttta ttatacattg attacctcct cactattatt
    32401 attcgaaaat aaggaagaaa aatgctaaaa tatatgaaat tgttctggga gaattaagat
    32461 gacaaatgta actagttata aagaaatgct ggctaaacct tggggaaaaa tacaatatga
    32521 aataactttt gctcaattaa gccatatcaa aaatcagaac gttttagatt ttggagctgg
    32581 tttttgtctc accgcacaac atctagcaaa agaaaacaat gtaactgcta ttgaacccaa
    32641 tcctaaactg ctttacgata atcaaagtga caatatttat aaaatcctag gttcctacga
    32701 agcattaaga gctttgcctg atcaaagttt tgatactatt atttgtcata atgttcttga
    32761 atatatagac aaacataatc atcctgctta ttttgacgaa ttttctcgac ttctaaaacc
    32821 taatggagaa ctatctctca tcaaacataa tataattggt aaaatattac aatcagttat
    32881 ttttagcaat gatacctcca ctgcgatgga actcttaaca ggagaagcaa actttaaaag
    32941 tgccagtttt gaccagggaa atatttatac cttagaagaa ctaaaacaaa atacaaattt
    33001 attagttgaa cgttatcagg gtattcgtac cttctattca ttacaaccaa accattttaa
    33061 aactgaaact gattggctaa acaaaatgct tgctatcgag ctaagtgttg ctgacaaagc
    33121 tccttataaa gatattgctt ttttgcaaca catcacactt aaaaagtcat tgtaacttct
    33181 taagtttaca actaaaaatc aacttagctg aagctaagtt gatttttact aatttttcca
    33241 atgtttattg gcgtcgaaag actctccaac aattacctga tcagataatt caatgatacc
    33301 tctgacttga ggagcatttg gaagagctaa ctcgcgagca ctacataaca ttccaaagct
    33361 atcttctcca cgaagttttc ctgggaaaat aaggctacca tttggcatca tagctcctgg
    33421 aagagctgca actgttttaa ggcctactga agcattaggt gccccacaaa cgatttgaac
    33481 cgtcttccca tcgttgattt ctacttgaca gatgtgtaaa tggtcactat ctggatgatc
    33541 aatcatttct ttaatttgcg ccacaacaaa tgtaggttca atattattaa caagagagtc
    33601 ttcgaatcct tctttcagta gttctgcatt aaggatttta ataacttgat cagataaagt
    33661 tacttgacct actccctcaa tagcaattaa acttgacact tcaaagatat tccaagcaac
    33721 tgtttcttta ctatcttgca gatagacgcg tgctacttgt ccgcgacgat caacatctag
    33781 cttagctcct tgactatcct taacaataac cattaaggta tcaccaacat gctctctatt
    33841 ataagtaaaa atcatttatt ctcctttaaa ttttgctatt ttttgtctta tttatggtta
    33901 atttcagcga gaaatttagt aatttcagcc tttgttttgc gattcttatt aaccaaacga
    33961 cccagttcct gaccattttc cacaacaacg aaactcggaa tcccaaaaat attccactgc
    34021 tgagctaatt ctatatagtc atcacgatta acacgtacaa agacaaaatc tgagaaatct
    34081 ttttcaattg atggcattac tggatatata aattgacaat ctggacacca atctgctgta
    34141 aaaaagaaaa caactttctt agtgctatca atataagctg ctatttcttc ataagattct
    34201 ggtaaaatca tttgctcacc tccacataat caagaacacc ttgacgataa atgaattcga
    34261 aaattctgcc atcttccaag acaagaccac cactggtcat tttaatatca gattcaaatt
    34321 cattaaaata aaatacctga atatcaccca tctgactaaa aagttctctt aaggtctgag
    34381 tgatttgctg acgtttataa tctgttactt ttttagttag ggtataacca gcagctgcca
    34441 gtaaacccac accagcaagg ccagtcccaa ataagatttt tttatttttc ataactgtta
    34501 ttataccata ccccccggaa ttagacacag gaattgatgg aagaaatcca cataacagtt
    34561 atgaaaaaaa acaacttatt ccttgtggta taataggatg acattgtctt aagataccat
    34621 tatggttaat cattcaaaca gaaagaaaaa acatgaaaga aaaattatta gtacctgctt
    34681 taaattgttt gctaggtcta ctcttcctag gtgatcactg gtttttattt cgtcttcaaa
    34741 atggagaact ttttgctatt gtgacgctaa tactgctttg cacagcttca tttttaatca
    34801 ataagaatca ccccaaaaat ttgactttaa ttgctatggt tatcactttt ttagccgtat
    34861 tatctttcct atctaccttt ttattagctt aagccgataa acattatctc cgttgtattc
    34921 aacccagttt tcactataac aggctttgtc agtcataaca gtcttctggt agtacttgat
    34981 attctttttg aaagtcttta tattgactga aacctgttct gttctttatt tttaattaaa
    35041 aggtgctaaa atagtatttg tagaattgag gtaattatgt cagatttatt taacaaaatt
    35101 aaaaccgtaa ctgagcttga tgggattgct ggctatgaac acaatatccg caacttcctt
    35161 cgtcaagaaa taactccttt agttgatcaa gttgagacag acggacttgg tggaattttt
    35221 ggagtaaaaa atactcatga gactaatgct cctaaagtca tggttgctgc ccatatggat
    35281 gaagtcggct ttatggttag tcatattcag ccagatggaa catttcgtgt acttgaggtt
    35341 ggaggatgga atcccctagt agtcagctca caacgcttta ccctctacac acgttctggt
    35401 gatgccattc ctgttatatc aggctcagtt cctcctcact ttcttcgtgg acaaagcggt
    35461 ggaacaacat tacccaaaat tagtgacatt gtttttgatg gaggattcac agataaaaat
    35521 gaagctgaaa gctttggcat tgctcctggc gatatcattg ttcctaaatc tgaaaccatt
    35581 ttaactgcaa atcaaaaaca tattatgtca aaagcttggg ataatcgcta tggtgtgctt
    35641 atggtgaccg aattgctaaa aagcttaaaa gatcaaagtc ttagcaacac acttattgct
    35701 ggggctaatg ttcaagaaga agtcggactt cgtggcgcac atgtttcaac aactaaattc
    35761 aacccagata tcttcttagc tgtcgattgt tccccagctg gagatattta tggggaacaa
    35821 ggcaaaatag gagagggaac cttaatccgt ttttatgatc ccggacatat catgcttaaa
    35881 gatatgagag atttcttgct tacaacagct gaagaagcag gtataaaata ccaatattat
    35941 gctgcaaaag gtggtaccga tgctggggct gctcacctaa aaaatagtgg tattccttct
    36001 acaactatcg gtgtctgtgc acgctacatt cattctcatc aaacactcta cgctatggat
    36061 gattttctac aagcacaagc ttaccttcag gccatcgtta acaaattaga ccgctcgacg
    36121 gtggatatta ttaaaggtta ttaagataga aaggatggga gtttcataag gtagacacat
    36181 tttagtatct cctttcatcc ccatatcaac attatgaaaa ttggaattat cggcgttgga
    36241 aaaatggcca gtgcaattat tcaaggcctc aaacaaaccc aacatgacat tgtcatatcc
    36301 ggatcttgct tagagcgttc aaaagaaatt gcagaaagac ttgatgttac ttacgccgaa
    36361 tctcatcagt cattaatcaa tcaagctgat attatcatgt taggtattaa acctcaacta
    36421 tttgaaaagg ttcttcttcc acttgatatc acaaaaccaa tcatctcaat ggcagcagga
    36481 atctccttag cgcgtttaag ccaattaact cgttctgatt taccattgat tcgtattatg
    36541 ccaaatatta acgcacaaat acttcaaagt tgcacagcta tttgttacaa caatcatgta
    36601 tcagatgagt tacgacaatt agccaaagaa attacggata gttttggaag tagctttgat
    36661 atagcagaaa tcaattttga taccttcact gctctagcag gttctagtcc tgcctatatc
    36721 tatctattta tagaagcctt agctaaagca ggtgtaaaat atggatttcc aaaagaacaa
    36781 gcactcccta ttgtaggaca gactgtctta gcttctagtc aaaacctatt acaaggacaa
    36841 aatagcacct ctgacttaat cgacaacatt tgtagcccag gcggaactac tattgctggc
    36901 cttttagact tagaaaaaaa tggtctgacg catagtgtta tttctgccat tgatgctact
    36961 attgagaaag caaaaaaact ttgagcaaat tgctcaaagt ttttttagtt attcatcatt
    37021 gtactgatta acacgctata agcaagagca ttgttaataa aatgaatcat tatactatat
    37081 tctaagtgct cagttttata ataaacaaaa gataaaatag cacccatccc agcataaata
    37141 ataaaactac ctatatcaga aggggaatga ataagagcaa aagctaaagt accgatgaca
    37201 aaaccccatt tttgatgttt cgcaaaaagt tctttaggga taatatagcg aaatataatt
    37261 tcttccataa ttggtgcccc tattatggca aaaattaaca tcaaagctag tggaagttgc
    37321 tttgttaatt ccataattac tgtttgattt gctgtatctt taaccccttg catagtcata
    37381 atgaggtaac caataatttg tggaaccctc aatgataacc aacctaagag aatagtaact
    37441 aatgctttcc ttgtcaataa tactttccat ctgattattt gtttaacctt ggcataataa
    37501 aggaaaagag caataataag taattctaag ccaattaaca atatactagt aaaaggcgtt
    37561 tgatagtgat tctttaggag ccacacagcc aatataggaa caagttgatt aataactaaa
    37621 aatgctatta gcaaagctaa aattttgcct tttttaataa taaatgacac aaaaacctcc
    37681 aaaataaatt attaactttt ttctatcata acatattttc aaatcttata gatttatctc
    37741 acaataactt ctgtaaaata aagtaaatta ataaataacc gtataaaaca aaagagagta
    37801 tcaatagcaa gaactttgca taggaaaaag ttataccaaa ccagacatta atcagacaca
    37861 ataccactaa aacaaggaca gataaaagga aaaggagtcg acaagacata ttagcaataa
    37921 attgattacg ctcatcaaaa actttggtat agtaatgttg aaatcgcttt tcactaaatg
    37981 ttatcaccaa aaaataaaaa cttcctgcaa ataaggcaat acctgaacca aaggaaaaac
    38041 ctttagtaaa attagattca aaggtatgta gaaatgatga tactatcata atcaaactac
    38101 atacactaaa aacaaaagac aaaaatctca tatatacttt gtaatccttc ggagatactt
    38161 tactattggg tgacaataat ccttttatta acatagaata ctactttcta aaacttcaat
    38221 ttaatcatca gctattatat cgttttcttc aatgaagcat agcgataaat agcaaaaata
    38281 gacatcgcaa tgctaatact aatagtactg ataaaaacat attgctggta aacgattggt
    38341 ggacaaaaat caatagtcca catccttgac atattacaga ttaactttta accttttgtc
    38401 ttttgtggac tgtatttttc cattaaaatt ctatcctcct tcactttctg tataaataaa
    38461 aacttcctca atttgtttat caaaataacg cgctatctta aatgctagct ccaaagatgc
    38521 cgtgtatttc tctttttcta gagaaataat agtttgccta gtaactccaa gtgcaactgc
    38581 aagttctgct tgacttaatt tcctagattt tcgtaacttt tgtagagaat ttttcatatc
    38641 ttacaccttt ttgtatagtt agctttacat ttatagtata gttaactata cattttttgt
    38701 caagtacatt ttacatacct gttcaaaaaa caaaaaagtt gatagttaaa ctatcaactt
    38761 ttctggtctt atcaattttt tgataactta tttagcttta aggcgctcaa cgtcacgcgc
    38821 aatcattaat tcttcatcag taggaataac taaaaccttc acttttgaat ctggttttgt
    38881 gatatctcca aaataaccaa aaacattttt ttctggatca agttcaatgc caaaccatga
    38941 taatcctgcg ataacatctt gacgcattaa tggcgcattt tctcccatac ctgcagtaaa
    39001 aataatagca tccgcgccat ttaatactgc caagtattgt ccaataaatt tcttaatacg
    39061 gtcaataaag acattatagg ctaacactgc atttggatct ttactttgaa gacctgcttc
    39121 aatatctcgc atatcacttg aagtgcctga tactccaagc aatcctgatt gtttgttaag
    39181 catattaaca acagcagctg catcttccaa ttctggatca ttagctacaa gataagggat
    39241 aatcgcagga tcaatgtctc ctgaacgtgt tcccatcatt ggtcctgcaa gtggtgtgaa
    39301 tcccatcgat gtatcaatag actgtccatg ataatttgct gtaatagaaa caccattacc
    39361 cacatgtgct gtgattaatt ttaattcttc taaaggacgt cctaattgct tagctgcttc
    39421 ttgtgcaacg tattggtggc ttgtcccgtg tgcaccgtat ttacgtactt tataatcagt
    39481 atagtacttt tgtgggattg gatataagta ggtatgaggt tgcattgttg tatggaaggc
    39541 tgtatcaaat acacatacac tcgtgatatc tggtaagatt tctctaaacg cacgaatccc
    39601 tgctgcagct gctgggttgt ggagtggagc taaagctgat aattcttcaa cctgttctac
    39661 aactttatca tctactaaag cagactcttt aaaatattca ccaccagcaa cgacacgatg
    39721 accaacacct gtaatttcat taaagtcttt gataatacca tgttttgtta gatcttctaa
    39781 aagaatttta acagcttggg tatgatctac gatatctaaa atttgttcat cttttttatc
    39841 gtcaaatttt acagtcgaaa tagaatcttt caaaccaata cgttcgataa tccctttggc
    39901 aactactttc tcctctggca tttcatataa ttgccatttc aaacttgaac ttccagcatt
    39961 aattgctatt gtttttgaca taatttacct ctctaaagcg ttttcacaat ctctattcta
    40021 tcatattttt tgagtaatga ctattttctc tgctccattt ttgaaaattt tcaatgaagt
    40081 ttgccatatt ctcacgattt tgcaaatctg tcagcggata tacaaaggtt tctggttttt
    40141 gttctgcttg cttcttgaga acaaatatag atttcgcatt ttgacgactt ccaaaaatag
    40201 tttctggtag agttaaaacg gcaatgacat ctgcatatcc ttttaaccac tccttcagca
    40261 aatcactttg tggacttgtt aaaaggtttt cgggtgctaa aaatatagcg attccatctt
    40321 ttttcaaata tttaagagat tgctccatca atagatggtg agcataggtg tgctctttag
    40381 aacttgatac agcatatcgt ttagcaatat catcattagg ataatagcca actggtaaat
    40441 cactaatgat tacatcgctt tctttcaaaa tttgtggtct aacggcatcc tcttggataa
    40501 attgggcact agaacctata atttcagcaa tgcttgctga tagatcaatc aaaagatcat
    40561 caacttcaat gcccatataa tttaactctt tcgagctgtt attgaggaga gtctgagcta
    40621 aattcccagt tccacttcca atttccaaga catccactgt ctcttgactc gttaattctt
    40681 ccaaaagaaa taacaagata aaaccaatac tatctggcgt aaactgatga ttagcttgta
    40741 attgctctgt ttgcgcagat ttgataaaaa tgaactggaa agtgcgacgc cattcttctt
    40801 gacttaagtc aagttgacgt aatttttggt tattcaccac aacaatatct aaatcacaac
    40861 ttgaaccaag gtaataagag ttctgttcaa ttaaggcatc ataaatatga gtttttaatt
    40921 ggttctcaat cgtttggata ttttctaaaa tcagctcata ggctgtctca attttttcaa
    40981 aattcatgat ttcctccaga atttatcata acaaaaaaga cagcatctga aaagagaagc
    41041 aaacaaaaat cctgttttta agcaaaacct tgtcttacta atatttcatc actgtatatt
    41101 tttaatgttt atggcttgct ttattatcat tagacttgag aggtacttgg agagtgtcta
    41161 ttttacgata aaacttatgg ttatagtttc ctcccttatc caatgtaacc ttaacttgta
    41221 aatagttccc ttcttggagg tatgaagcat atcctcctgt aaaataacat tttcctgata
    41281 tggaatcgcc ttctattgtt ttataagcta actgcgctgt tagataagct ttacttgctt
    41341 caatttggct atgatactgt ctttctgttg ccaatattct tgctagataa aactgtaaga
    41401 cgagtgtaaa tacagcagcc aatacaatag cttgcaaaag gatgccagct ttaagtttct
    41461 tctttaaaat catagtaaaa tgtccttttt aacccgtcct taaaataaaa aacaagtttt
    41521 accatacttt tggtctgact catttgacaa ttgtctaacc cataaaccat tggttgataa
    41581 cctcgaccat tataacctgt cttacggaaa tcatctttat tagatttgcc aaaggttaca
    41641 atcttatctt gcttacgtaa ataaagtttg ttctgtctta aatattccaa gtgagcgcct
    41701 tcgaattctg catttagttg ctgagttaat aacacccatt cagactgact ggaagaagac
    41761 atcactacta tctgttgagc caacaatttt gttagtcctt gataaactag caaagctcct
    41821 gtgattgtaa ccaatgctac caaacattct aaaatgtgta aatgctttaa ccttcttatc
    41881 cttacatttt aacaataaat ttttcatgat aatatctcca tattttgata cattggtaac
    41941 agcattgctg cataaatcat aacaatgata agagctacaa aaataaaaat aacgggttgg
    42001 attaattggg tcgctctagc taattttgta aaaaaatcct cccacttctc atcagcatat
    42061 atatctaact ctgttcctaa tttcgcctta acttgaccat attcaatcat taaactaagc
    42121 tcagttaaga aaaacggata gtctaatact ttttggtgaa atgctttacc tgatagaaaa
    42181 ccttcttcca tgtcatatcc tatttcccta aaaagtttgg atttctgatt ttgcattact
    42241 ttaacaattt ggtccaattc aacaccttga cttaataaat ttccccactc acgggcatag
    42301 taagcagtta aataaagctt aacatatgat ccaactaagg gaattgttgt taaaaaacaa
    42361 gctactttaa tgcgcgacaa tcgcttttga ataatataaa atattaaact aaaaataagt
    42421 acaactgcta aaagtaataa gaaaatattc ggcacatttg taatcagtct agttgcaaaa
    42481 ttattttctc ctaattgggg cattaaataa tgcctaaggc caatcataat tagcaccaga
    42541 aaagacaata atatcaatgg gtaagtagct acttctatta ctttttttct aactaacaaa
    42601 agattagcta aataagactc aatctttagt aggctccttg aaatgttacc atgcaaatca
    42661 gctaatgcaa cctgtgtgat aacagtgtca gaaaaaccta actctcctaa catttttgat
    42721 aaatcttttc cctctaataa tgccttattc atcctatctg tataacaatc agacaataac
    42781 ttactcctct ttaagaaggt gaccatatca gttaaagaaa aaccactagc aaaaagatta
    42841 ttaaaaagtt ggactacttt ccgttgtttc ttgagggata attttttcga cttgtgcctg
    42901 tttcttactg atatgtcctt cttcagccaa gatatccact tgtctattcc acttgtctga
    42961 tgagtgtttt ttaaagttac ttgtctcaaa gtcaattagg cttcctcctc caattaaacg
    43021 ttgatatgct attaatttta gactattttc taactcttga tagttaaccc ctaattctat
    43081 aagcctatca tagactccgg gaatactttt agcatgaata gtagaaaaaa ccattactcc
    43141 cgttaaactt gcacgaataa cagcacgggc cgtcgcttga tctctaatct ctccgataat
    43201 taaaatatct ggacgatgcc gtaaagacag tttgattaaa gcatcataag tcattccaat
    43261 atcctcattc aattggagtt gtaacatctt gtcattcttg atttctaccg gatcttcaat
    43321 cgtgataatt tgcttatttt taaatacttc tgaagctaat tgatacatga gagttgtttt
    43381 accactcccc acagggccgg aaaaaagata tagccctctt gcacacagta cttccttcat
    43441 ttgctttata ttatcaaacc aatattttaa gtcctgatga cctgaataca aagtacgaat
    43501 aactaaagat tcttgaccac gataatctcc cacacttgat agtcgtaatg aaaccagtct
    43561 tccctctgac agttcatagt cacaagaacc taattgactt cgtctttttt ctccaacgtt
    43621 catgcctgcc acaaatttaa agtgactaat aagactagcc atcctattaa actcaaaaac
    43681 atcaataaac cgcctttcat catcaatacg catatagagt tcataacaat cacctttggg
    43741 aatgatataa atatcttgag catttacttc tactgcctga tgaatgacgt gctttgctaa
    43801 tgattgaacc atcccgtacc tccttaacca tatattcgaa aataaaataa aaaaatctca
    43861 ccaattggtg agattttttc aatgatttct tgaggggtag taacataaat attatgaagc
    43921 agaggctgaa caaacctatt atatccccat aaaaactatc taactttttt aaactacttt
    43981 taattacaaa tttaaagaac atgccgatgg caactcagct atcttattaa atacttcaaa
    44041 tcgctcaata gattcttcct taggaatttc ttgccattct ttcaataaca ttaaggaatg
    44101 atactgttgg aggtactcat cacattcttc acaccaacgt ttttctttta tactccaaaa
    44161 cgttgccaac aaactagact tactttgaaa ataaggccac ttttcctgtc ccctatccca
    44221 ctcctcttga aaatagagca aagcttctga taaggtatca tactcagcaa tttcagtaat
    44281 atcttcctcc caaccttcaa taaaccacca tggttcccaa tcaccaaaca tttttacaac
    44341 ttgatacata atacacttcc tttagagagt attatataaa aaataagaat aaaataaaaa
    44401 atatctgctc attccaaatt cttgtaaaaa aagaacctga gttaaactca agttcttttt
    44461 gattaatctt ctgttgtagg tgttgatact tccgtaactt ctgcatcaac aggagttcct
    44521 tcaataatct caacttcatt aacagcaagt ggttcaatat tacggtaacg agccatacca
    44581 gtacccgctg gaatgatttt accgataata acattttctt taagaccaag taagtgatct
    44641 ttcttaccac gaatagcagc atctgtaaga acacgtgttg tttcttggaa ggatgcagct
    44701 gataagaatg aattagtttc aagggaagcc ttagtaatac ccataaggac aggacggctt
    44761 gtcgcaggga ttccaccaga aataacaata tctttgttag catctgtaaa gtctgaaata
    44821 tccataagtg tacctggaag aagatctgta tctcctggat ccatgacacg aactttacgg
    44881 agcatttgac gaaccattac ctcaacgtgt ttatctccga tttctacccc ttggctacgg
    44941 taaacttttt gtacttcagc aagtaggtac gtttcaacag ataaagtatc acgaacttca
    45001 agaagacgtt ttggttggat agatccttca gttaaggccg caccacgagc tacttcatca
    45061 ccaacttcaa ctttcatacg tgctgtaaat ggaacaacgt attctccctc acctgtctga
    45121 cctttaacga atactttctt agtacgtgtt gaagagtctt cttcaattgc aacgacttca
    45181 cccttaactt cagtaatgac tgcctcacct ttagggttac gtgcttcaaa gatttcttgg
    45241 atacgtggaa gaccttgtgt aatatcagta tttgaagcaa caccaccggt atggaaggta
    45301 cgcattgtaa gctgtgtccc tggttcaccg atagattgag ctgcaatagt accaactgct
    45361 tcaccaacct caacagcatc acctgttgcc aagttaatac cgtaacagtg acgacaaaca
    45421 ccatggcgag tgttacatgt aaatactgat cggatagtta cttcctcaac gcccgcttta
    45481 acaactttag cagccatatc ttcagtaatt aaggtatccg caccaacgag aatttcacct
    45541 gtctcaggat gtttgattga cttcttagta taacgtccta tcaaacgctc ttcaagtgtc
    45601 tcagtaacct ctttaccatc tgtaattgct gtgattgtca aaccgcggtc tgttccacag
    45661 tcatcttcac gaataataac atcttgggca acgtcaacca aacgacgagt aaggtaacct
    45721 gagtcggctg tcttaagggc cgtatcagtc atacctttac gcgcaccgtg agttgagaag
    45781 aacatttcca aaacactaag accttcacgg aagtttgata agattggcaa ttccatgata
    45841 cgtccgttag gagcagccat caaaccacgc ataccggcaa gttgtgagaa gtttgagatg
    45901 ttaccacgag ctcctgagtc catcatcata acgatagggt tctttggatc ttgtgtctca
    45961 ataagacgtt tttcaagtgc ttctttagct tcacgccatg ttgttgtaac agcaacataa
    46021 cgatcttctt cagtcatcaa acctcgacgg aaggctttat tgatatcttc aactctgtgg
    46081 tgagctgcat caataatctc agctttattg tcaataactg ggatatcagc aatacccaca
    46141 gttaaaccag caagtgttga atgataatat ccaaggtcct tcaaacggtc aaggaaagca
    46201 gaagtttctg ttgtacggaa acgtttgaaa gtttctgcga taatattacc aagatttttc
    46261 ttcttaaagg gaatattaat ctctaagtta tcaataactg cttgaatatc ctgacctggc
    46321 tctaagaagt atttatctgg agttttttct gtcaagtttg cattattagg ttcaattaag
    46381 tacggtaaat cttcaggcat gatatcgttg aagagaatct taccaactgt tgtaaccatg
    46441 attttatgtt tttgctcttc tgtccaaggc ttattcggca ttgaatcaac agcgatacca
    46501 acacgtgtat gtaaatgaac ataaccattt tgatatgcca taactgcttc gtcatgatct
    46561 ttgaaaatca tgccttcacc ttcacgacca gcatcttcca tagtcaggta gtaatttcct
    46621 aaaaccatgt cctgagatgg tgttacgact ggcttaccat ctttagggtt aaggatgtgc
    46681 tctgcagcaa gcatcaaaag acgagcttct gcttgggctt cttcagacaa tggaacgtgg
    46741 atggccattt ggtctccatc aaagtcagca ttgtaggcct cacatacaag tggatgaaga
    46801 cgaagcgcct taccatcaat aaggacaggt tcaaacgctt ggatacctaa tctgtgaaga
    46861 gtaggtgcac ggtttagaag tactgggtgc tctttaataa cttcttctaa gatgtcccaa
    46921 atacgctcgt ccccacgttc aaccatacgt ttagctgctt taacgttccc tgcgaggtca
    46981 cgagcaacga tttcacgcat tacaaaaggt ttgaagagct caatagccat ttcacgtggt
    47041 acaccacatt ggtacatttt aagtgttgga ccaacagcaa taacggaacg tccagagaag
    47101 tcaacacgtt taccaagcaa gttttggcgg aaacgacctt gtttaccttt aagcatatgg
    47161 cttaatgatt tcaaaggacg actacctggt ccggtaattg gacgaccacg acgaccattg
    47221 tcgataagcg catcaacagc ttcttgtaac atacgttttt cattttgaac gatgatgcca
    47281 ggagcattta attccaacaa acgtgccaaa cgattgttac ggttaataac acggcggtaa
    47341 aggtcattca agtctgatgc cgcaaaacga ccaccatcta attgaaccat cggacgaaga
    47401 tccggtggga taaccggtaa aatgttaaga accatccatt ctggtttatt tccagatttt
    47461 ttaaaggcat ctaacacatc cagacgacga actgctttca cacgtttttg acctgtcgct
    47521 gatttcaact cctctttaag aactgcgatt tctgcatcta aatcaacgcg tttaagaaga
    47581 tcttgaatgg cttcagcacc cattttagca acaaacgaac cgtaaccgta ctcttgaagt
    47641 ttttcacggt attcacgctc agtaaggagt gacttcggtt ctaatggtgt gtccattgga
    47701 tcgataacaa cataagccgc aaaataaata acttcttcaa gtgcacgagg actcatatcc
    47761 aatgtgagtc ccatacgcga tgggatacct ttgaaatacc agatatgaga tactggtgct
    47821 tttaactcga tatgtcccat acgttcgcga cgaacttttg cacgagttac ttcaacgcca
    47881 caacggtcac aaatgattcc tttgtaacga atacgtttgt attttccaca cgcacattcc
    47941 caatcttttg ttggaccaaa aataacttca tcaaaaagtc cttcacgttc tggtttcaat
    48001 gtgcggtagt tgattgtttc aggtttttta acttcaccat aagaccatga acggacctta
    48061 cttggtgatg ctaatgtgat ttgcatactt ttaaaacgat ttacgtcaac cactagtttt
    48121 acctttcttc gatttatcat catactagcc cagctaggat ttcaataaat cctaggacta
    48181 gcattgcata tcaattctat attcgcttct agttataaac tagataaatt taatcttctt
    48241 gaacgacttc agatacttgt tcagcttgtt tttcttcagc ttcttgtttc acacgcgctt
    48301 tttcaaggtc atcaacgtgc atcacatcat catcttcacc ttcatcaaga tcacggagtt
    48361 caacttcgtt gtcatcttca tcaaggacac gcatatcaag accaagtgat tgcaattctt
    48421 ttacaagaac acggaatgat tctggaacac ctggttttgg aattggctta ccttttgtga
    48481 tagcttcata agctttaaga cgtcctgtaa catcatcaga tttgtatgtt aaaatttctt
    48541 gaagaacatt tgaagctcca taagcttcaa gcgcccaaac ctccatctca ccaaaacgtt
    48601 gtccaccaaa ttgagcttta cctccaagtg gttgttgagt aacaagtgag tatggtccta
    48661 ctgaacgggc atgaagttta tcatcaacca tgtggtgaag tttgatcatg tacatgacac
    48721 caaccgacac acggttatcg aatggttcac cagtacgacc atcataaaga acagtcttag
    48781 catctgaatc catacctgct tcttgaacag tttcccaaag gtcttctgaa gaagccccat
    48841 caaagactgg tgttgcaata tgaataccta aattacgagc agccatacct aaatgaagct
    48901 ccataacttg tccgatattc atacgtgatg gcaccccaag tgggttcaac atgatatcaa
    48961 ctggcgttcc atctggtaaa taaggcatat cttcaacagg aacaatacgt gagacgacac
    49021 ctttgtttcc gtgacgaccg gccatcttat caccgacttt gattttacgt ttttgagcga
    49081 tataaacgcg gacaagcata ttaacacctg attgcaattc atcaccattt gcacgagtaa
    49141 agattttaac gtcacgaact actccatcgc caccgtgagg tacacgtagt gaagtatcac
    49201 gaacttcacg tgatttatca ccaaaaatgg catgcaagag acgttcttca gcagataagt
    49261 ccttttcacc cttaggcgtt accttaccaa caagaatgtc accttctttt acctcagcac
    49321 caatgcggat aattcccatt tcatcgagat cacgtagtga atcttcacca acatttggaa
    49381 tttcacgagt aatttcttca ggcccaagct ttgtatcacg cgtttcagat tcgaattctt
    49441 ccaagtgaac agatgtatag acgtcttctt tcacaaggcg ctcactcatg ataacggcat
    49501 cctcgaagtt ataaccttcc caagtcatat aagcaacaac tgggttttgt ccaagagcca
    49561 tttcaccatt ttccatagat ggtccgtcgg cgataaaatc acctttttca acgagatcac
    49621 caactttaac taaggtacgt tggttataag cagtacctga gtttgaacgg cggaattttt
    49681 gaacatgata aacatcaaga gaaccatctt cgcgacgcac ttcaactttt tcagcatctg
    49741 aaaaaataac acgaccgtca tgtttagcaa tcacagctgc acctgaatcg tgggctgctt
    49801 gatactccat accagtacca acatatggtg cttttggatc aatcaatggc acagcctgac
    49861 gttgcatgtt ggcacccatc aaggcacggt tagagtcatc attttccaag aaaggaatac
    49921 atgctgttgc gacagcaact acttgcttag gtgacacgtc aacaaagtca acaatgcttg
    49981 aaggaaactc ttggttatta ccttgatgac gacccataac gatttcttct gcaaatgtac
    50041 cgtcttcgtt aagcttagag ttggcttgtg caactgtaaa ttcatcttct tcatcagcag
    50101 tcaaccaaac aatttcgttc gttaccgcac cagtagaacg atctacttta cgatatggtg
    50161 tttgaataaa tccatattta ttgaggtgtc caaatgaaga taagttatta atcaaaccaa
    50221 tgtttggacc ttcaggtgtt tcaattggac acatgcgccc atagtgggta tagtgcacgt
    50281 cacgaacttc atatccagca cggtcacgtg tcaaaccacc aggtcctaag gcagagagac
    50341 ggcgtttgtg cgacaattct gatagagggt tgtgttggtc catgaattgt gacagctgtg
    50401 atgaaccaaa gaattcttta acagcggcag tcactggacg aatgttgatg atttgttgag
    50461 gtgtcaagac ttcattgtct tgtacagaca tacgttcgcg gacattgcgt tccatacgag
    50521 caagtccgat acggaattgg ttcgcaagta attcaccaac tgcacgaata cgacggttac
    50581 ctaaatggtc aatgtcatct accttaccga taccctctgc aaggtttaag aagtatgaca
    50641 tctctgctaa aatatctgca ggagttaagg cacgaacttt atcttcagga tttgagtttc
    50701 caacaatagt aacaacacgg tctggatcag ttggagcaac aactttgaat ttttgaagaa
    50761 taactggttc agtaactaca gcataatcat ttggtgtgta gacaaacttg tttaagtctc
    50821 catcgatatg ctctgcaatt gaatcaatga catctcgtgt cataacggtt ccagcttcaa
    50881 caagaatctc tcctgtttca ccatcaacta aattttcagc gattgtttgg ttcaataggc
    50941 gtgttttaag attcagtttt ttattaatct tataacgacc aacagccgct aagtcataac
    51001 gacgtggatc aaagaaacgt gcaactaaaa gactacgtga gctgtctgca gtttttggtt
    51061 cacctggacg aagacgttca tagatttctt taagtgcttc atctgtacga gaatcacttg
    51121 gatttttatg aatgtctttt tcaatagtat tgcgaacaag ttcactgtca ccaaagatgt
    51181 caacaatttc atcgtctcca gagaaaccaa gtgcacggac tagtgttgta aatggaattt
    51241 tacgtgtgcg gtcgatacgt gtataagcaa tatcttttgc gtctgtctca agttccaacc
    51301 aagctccacg gttagggata acagttgacc cataaccaac ttttccattt ttatctacct
    51361 tatcattaaa gtaaacacca ggagaacgga ctaattgtga aacaatgata cgttcaccac
    51421 cattgatgat gaaagtaccc atttcagtca taattggaaa atcaccaaag aaaacttctt
    51481 gagttttaat ttcaccagtt tctttattaa ctaaacggaa agtaacaaaa ataggtgctg
    51541 agtagctagc atcatggata cgagcttctt ccaaagtata tttaggttct tttaattcat
    51601 aaccaacaaa ttcaagatcc atagtatctg taaagtttga aattggaagt acatcctcaa
    51661 atacttcttt caagccagca tcaagaaaat cttggaatga atcagtttga atttcaatta
    51721 agtttggtaa atcaagaact tccttaattc ttgaaaagct acgacgtgta cggtgttttc
    51781 cgtactgaac ttcatgtcct gccaagtttt tagctccttt ttaaagatag agtgactaag
    51841 tttttatctc ataaaactta gtgagtttat ttcatttgct cgcctaacaa gaacaagctt
    51901 atagatgtcg tttgtggtaa ttttcagaaa gtttaaatat atattacaat aacctttttt
    51961 cttaaaatta ggcacaaaaa gagcagctaa atctcacttt atcaagttga tttaactgct
    52021 gtatatctag gtcggacaat atttcaacct agacagacga caaatagtta cgtacattat
    52081 aacttatttt caccaataaa tcaagtttta ttctcttgac ttcaaggaag cttctagaat
    52141 ttcaatttgt tcatcaacac ccttgagttt tagcataaca cgatgatttt taaacatagc
    52201 atactggtgt ttttctaatt ctttgaaaga agactataca agaattaaat catattatct
    52261 tggaaaaaat aaaaggagaa cagatcactc tattctcctc aaataaaact atcagtatcg
    52321 taaacctgtt atcgttttcc acccaaagta gaccaagctt tttgataatc actatcagtt
    52381 cccccaatag caaaacgata ggtcatcgtt ccaggtccat tttttgccca ataacttgtt
    52441 gtgctttcac ctccaacagt gattcgacga ccgtttactg ttactacacc tggctgtaaa
    52501 cctgtagact taagaacttt agcttttata acgctatcat caagtctaaa cctttctgac
    52561 ttaccaaaag tattgccatc tgcattatta atagcattaa ccaagtgagc catataatta
    52621 gcattattat tgtaacctgt caatttcgct aaagaggcat tattatcatg tcctgcccat
    52681 ccacctaaag tcactttagg tgtagaaagc atcaaccaaa catcagaagt tgagttagta
    52741 gtacccgttt ttccaatcca atctacccca gctaaaccac tatttaaacc ttgtaagcga
    52801 tttttaaagg ttgtagtctt tccggagtta attggtccat gtaatagctg ctgtaagata
    52861 gtagctgttg ctttcgaaaa gacacggaca ggtttacttt cgtgattata aatcactttc
    52921 ccattactat cctctatact ttctatcata tactgcttat gataaacacc accatttgca
    52981 atcatttgat aaaggtttgt ttgctgagca actgacgtat caataccacc accaagtggt
    53041 aaactttcta taccaaagtt ttcaattgga tagtctaatt tctccatata gttttttaca
    53101 tcaacgcctc gatctcgtag catcttgtaa gtccagaaag ctggaatgtt ccaagaaatg
    53161 tccaatgact cttgaaggtt aaccatagca gtaccttctt catccgcatg cataatcttt
    53221 tcgccacttg aatatgtcgt tggataattt gaaagaacac taccacttcc taacatccct
    53281 tgatcaatag caattccgta aggtaatatc ggtttaatac ttgaccctgg agagcgtgcc
    53341 gtatcaaaag catggttatt ttgattctca ctatagttac gaccaccaat aaatcctata
    53401 atagcaccac tagaattgtc cgttagtaca tttcccattt gaaccttgcc agtaccatca
    53461 tctaacaacc ctccgtattg agctgcagca tcctgcatgg cttgataaac agatttattg
    53521 atagtagttt taatagtata ccctccctgt tgaatctctt caatagcgcg gtgtctgtac
    53581 gtcgctctag tctcatcatt ttttaaatca tgctctgaaa cattatcctt tttaatgagg
    53641 taattgtaca tcactttttg agcttcagat aatgccgagt agtaaagata atcatgatga
    53701 tttgttgtcg ccactgcagg tttgataaaa tcttttttga tgtcataatc tttgtaggat
    53761 ttgtactcat ctttggttaa agctctcgtt ctatacatat tgtataacac atttttttgg
    53821 cgctttatac caaaactaag atctttatct gatttcaact gggcatctgc agtataagga
    53881 gaatatacaa taggactttg cggtaaacct gcaaggaaag ctgcctgagg aattgttaaa
    53941 tcttttgcgg aaactccaaa aatcccttgt gctgcttctt caatacctgc aatattctgt
    54001 cctttgttat tgcgaccaaa cggtgataca tttaaataat ctgataaaat cgaatcctta
    54061 tccatgtaac gctctaatgc tagagcataa ataatttctt ttgatttgcg tttaaatgac
    54121 ggatcatctc ccaaaatttg ctgctttaat aattgttgtg tcagcgttga accaccgcta
    54181 ctctctccaa accccagaac agatcccgct gcagcacgta gtacggcttt gggaactaca
    54241 cctttatggt cattgaaatt ttcgtcctct gttgcaatga tagccttttt aatattatca
    54301 gatatagcat cctttgccac tggtgtacgt tgtaaatcag tggcaatctc tgaaatttgt
    54361 gatttatcag aataagttaa cctagacacc ctagtaagtg tattaacttg tgtcactaaa
    54421 ctatttttac ttggtacttt aacagagtca acttgactag caagataccc tactgcaagt
    54481 cctactccga gcataacaaa tagtaaaata ataacataga tgaaatctga taaaagcttt
    54541 acaattctta aaaaaataga accaaattca agtggtgtgt aatcacctaa tttagaacta
    54601 ttcaacttct tattaccttt aaacatgaat aatacctcat cctacattat atcagattta
    54661 atataacttg catatcctaa agagttttga tatagttgac attaatacac aacaagttaa
    54721 taattaggag aaaaagatga atatttttga tgaactcaaa gaacgtggac tcgtttttca
    54781 gacaactgac gaagatgctt tacgcaaagc tcttgaagaa ggttctgtct cttactatac
    54841 tggctacgat cctacggctg acagccttca tcttggacat ttagttgcta ttttaacctc
    54901 tcgtcgctta caacttgctg gtcacaagcc ttatgcactt gtgggaggcg caactggact
    54961 tattggggac ccttcattta aagatgttga gcgcagttta caaacaaaga aaactgtagt
    55021 tagttggggt aataaaatcc gcggtcaatt atctaatttt ctagagtttg aaacaggtga
    55081 taacaaagct gtattggtaa ataattatga ttggttcagc aatattagtt tcattgattt
    55141 cttacgagat gttggtaaat attttactgt caattatatg atgagtaaag aatctgttaa
    55201 aaaacgtatc gaaacaggga tttcctatac tgaatttgct tatcaaatta tgcaaggata
    55261 tgacttttac gaattaaata agaactataa tgtcactttg caaattggtg gttctgacca
    55321 atggggcaac atgactgctg gaaccgaact tattagacga aaatcaaatg gtgtatctca
    55381 tgttatgact gttccattga taacagattc tacaggaaaa aaattcggaa aatctgaagg
    55441 aaatgctgtt tggcttgatg ctgacaaaac ctctccttat gaaatgtacc aattttggct
    55501 caatgttatg gatgctgatg ccgttcgctt ccttaagatt tttacattcc taagccttaa
    55561 agaaatagaa gatatacgta ttcaattcga ggaagcacct caccaacgct tagcacaaaa
    55621 aacactagca cgtgaagttg taactcttgt tcatggtgaa aaagcttata aggaggccgt
    55681 taatatcacg gaacagctat ttgctggcaa tataaaagga ttgtctgtta aagagctcaa
    55741 acaaggttta cgtggagtac caaattatca cgttcagacg gaagataacc tcaatatcat
    55801 agaccttcta gtcacttcag gagttgtcaa ttctaaacgt caagctcgag aagatgtctc
    55861 caatggagct atttatatta atggagatcg tattcaagat ttagaatata ctatctcaga
    55921 aaatgataag cttgaaaatg aaattacagt cattcgtcgt ggaaagaaaa aatacttt
//
LOCUS       DATROQ010000002        53537 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.002, whole genome shotgun sequence.
ACCESSION   DATROQ010000002 DATROQ010000000
VERSION     DATROQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53537
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.002"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            97..288
                     /locus_tag="VBC94_000057"
     CDS             97..288
                     /locus_tag="VBC94_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO6978370.1"
                     /translation="MPDIANGRKKICDFLKENNIKKAALAAAYGMSRQEVTNILSGST
                     RGLKANQFILRVIEDYKID"
     gene            322..1092
                     /locus_tag="VBC94_000058"
     CDS             322..1092
                     /locus_tag="VBC94_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEO6978371.1"
                     /translation="MNEIFNFHGQEVRTVTINNEAYFVGKDVADILGYSNGSRDVNRH
                     VEEEDRLKYRFGTSGQDREMTIINESGLYSLILSSKLPQAKDFKRWVTSEVLPTIRKH
                     GLYAIDDLLENPDMAIAALQKLKEERQLRLQAQEEVAQKNQIIQELQPKATYYDLILQ
                     SESLVAISVIAKDYGMSAKKLNSLLHELKVQYKQGNTWLLYQKYASKGYTQSKTHPID
                     AERSKMHTYWTQKGRLFIYDLLKNKKGILPLIEQEEVA"
     gene            1170..1481
                     /locus_tag="VBC94_000059"
     CDS             1170..1481
                     /locus_tag="VBC94_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO6978372.1"
                     /translation="MPKAEIIYRPANQSEKATHGDYAHLCQVWEGLTIGTAKVWAAEM
                     REHPDFRQFIDNPTHRIVFIDYEGFRLFVKWKSRNRYRPKKETLSEMIDNIKREKQLG
                     V"
     gene            1486..1632
                     /locus_tag="VBC94_000060"
     CDS             1486..1632
                     /locus_tag="VBC94_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978373.1"
                     /translation="MKKLLKKLFVKKNRKIDVRPAWKIETHGWEANARRYDNMIEHNR
                     GRTC"
     gene            1761..2172
                     /locus_tag="VBC94_000061"
                     /pseudo
     CDS             1761..2172
                     /locus_tag="VBC94_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2320..2523
                     /locus_tag="VBC94_000062"
     CDS             2320..2523
                     /locus_tag="VBC94_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978374.1"
                     /translation="MNYLEYALAYLERELEIIDNEAIEVELPDGDWEFVLNPYYEEGL
                     HDNPYYRSQVAKDILDIKGLLGR"
     gene            2613..2912
                     /locus_tag="VBC94_000063"
     CDS             2613..2912
                     /locus_tag="VBC94_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978375.1"
                     /translation="MIEVTFKAESMAGVFDAMREALDMPKTVTKDVVEEKPAPKKKEE
                     SVTLTLADIKKMAKAKVEEGKSKDIKEVLREMNVAKVGELEEYQFAEFVEKLEAL"
     gene            2912..4069
                     /locus_tag="VBC94_000064"
     CDS             2912..4069
                     /locus_tag="VBC94_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HEO6978376.1"
                     /translation="MPVENHALLSASSAHRWLYCPMLPRLEADYPSRDTVYTQEGTSA
                     HELSEIKLMYKSGKITKRKFNTLTKAFKENSDFYNEEMEEMTELYTDIVMEHLNAYEN
                     AEIELEKRVDFSDWVPGGFGTSDVVILADGVIEIIDLKYGKGMPVSANQNPQMGLYAL
                     GAYASYDMVYDFDRIKMTIIQPRLDSVSSVDIYVEELLYWADNVVLPMAAQADAGIGD
                     WNLSEKVLQWSPVAAKLVPRAQENWELIDKYDYQEPVYLSDEAVAEILDKASAIKKWV
                     ESVEAYALKEALSGKEVPGYKIVEGRSNRVITDKDKAIDILQDNGFDDEIFKPKELLS
                     MGALEKLIGKTTFADLLAEVIDKPQGKPVLVPEKDKRPAINSLEQAIKDFE"
     gene            4083..4646
                     /locus_tag="VBC94_000065"
     CDS             4083..4646
                     /locus_tag="VBC94_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HEO6978377.1"
                     /translation="MTTTPNTTKVVTGKVRLSYVALLEPKAFEGQEAKYSTVILIPKT
                     DKATIKKIKDAQKAAYEAAKDNKLKGVKWERVKTTLRDGDEEMDTEEHPEYAGHMFMS
                     ISSKTKPQIIDKYKNSVDSAEEVYSGVYARVSLNAYAYNTAGNKGISCGLNNVQIVAK
                     GDYLSGRSSADADFDEWNEEEDEDDIL"
     gene            4689..6611
                     /locus_tag="VBC94_000066"
     CDS             4689..6611
                     /locus_tag="VBC94_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEO6978378.1"
                     /translation="MRHLNIDIETYSSNDIKNGVYKYADAEDFEILLFAYSIDGGEVE
                     CLDLTRQSLPEDIKDMLFDDKVRKHAFNAQFERVCLSRYLGLPYYLDPCQWQCTMVLA
                     QELGLPSSLEKCALYLKLAQEKDTSGKNLIRYFSLPCKPSKTNGGRTRNLPEHAPEKW
                     QMFIDYCIQDVVVEMAIAEKLESVPVHDREWDYYVCDQRINDRGVALDKELVASALYC
                     KDVKMESLSGELKALTGLDNPNSRAQLLPWLKEHGYSANGLTKADVERELKTAEGELK
                     RVLELKLQTAMSSLKKYEAMERAMCSDGRVHGLLQFYGASRTGRWAGRVVQVQNLARN
                     YIKDLDDAREYVKKRDIDAVEILYDSLNDTLKQLVRTALVAKDCCTFYVSDFSAIEAR
                     VIAWFAGEQWRLDVFSTHGKIYEASASQMFGIPIEEIDKELRQKGKISELALGYQGGP
                     GALKQMGALNMGVKEEELQGLVDDWRRTNKKIVQFWKDVQRAAIKAIKSRAPIKLGKL
                     RFRYRKGFLFITLPSGRNLAYARAKVEPGDFGDKIIYEGQGDKAYFTAQETYGGKLVE
                     NIVQATARDILAEALLRIEAAGHGVVFHVHDEAIIEGSGLTIEEVNELMAQAPDWAEG
                     LPLNSEGYITKYYMKD"
     gene            6616..9000
                     /locus_tag="VBC94_000067"
     CDS             6616..9000
                     /locus_tag="VBC94_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO6978379.1"
                     /translation="MKQEKLIVKSSPLQELHIATGNSRTAKTWKNITLTWQELVERLE
                     KPTVTQETFAEYQKMSRAEKGQAKDVGGFVGGWLKQGKRKNENVQSRSLVALDADSPS
                     KDFLDRLDLLADYAYVLYSTHSHSKKAAKYRLIIPTDRLMMPDEYEPVARYLANQLGM
                     SNFDDTTYQSVRLMFWPSHSRDADFTFKYNDEAFLSVDEVLDTYPDWHDSSFWPESPT
                     HAVKRQREAKKQGDPLSKKGLIGAFCRNYDIRQAIATFLPEVYEEGTTPDRYTYTEGS
                     TANGLVIYDDVFAYSHHGTDPVGDTLVNAYDLVRIHKFGEQDSGAKDNTPTNKLPSSK
                     AMNAFVCDLPEIKDYLMAEALGDFDEELPVEDDRSWLEIDERGEPEVNSYLLATQIIK
                     EVPIYWDGFEFLRYDAKKGIWLPNAEEYLKSYISTKKLGKITKIRHISETIVAIKAQA
                     FSSEVFTESDLNKIVLANGVYDLRDNSFKTKFDPELHARSSHPVVYDPEATCETFEGF
                     LRETVGAENIDFIFEWFGYNFYREYTIQKMLFIYGSGGTGKSTLINILREMIGADNYS
                     AVTLQYLMQERFAKIGLYRKTANFDTDAKPQYLADGATLKMLTGEDTIHADRKNKEPI
                     NFYNYAKLSFAMNELPPMRDFSGGIKRRMMILEMDKVLTQEVKAKYPLDKIMGEVSGI
                     FNRAMEGLRKALSKRDFSISASMRSSVEKWEKGNDVVAMFLEDECEPGEDFKAPVRDV
                     YPAYKFYCQDSGYKPLAKNAFNHRLRELNYENKNVKSGGKQAKNWVGFRLKSEF"
     gene            9387..9662
                     /locus_tag="VBC94_000068"
     CDS             9387..9662
                     /locus_tag="VBC94_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEO6978380.1"
                     /translation="MRTEKDIENYLKKKTKGLCLKFTSPGTVGVPDRIVVMKTGVFFV
                     EVKAPGKKPRPSQILMHKKIKEVGQPVWVVDSYESVDIALKEMENWV"
     gene            9659..10981
                     /locus_tag="VBC94_000069"
     CDS             9659..10981
                     /locus_tag="VBC94_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEO6978381.1"
                     /translation="MRLHEYQEYAKTWIVEHPYCGLLLDMGLGKTLTTLSAIDEIQNI
                     FSEDHKILIVAPKKVAEETWPTEIGKWHFDFTYSKVLGSEGKRIEALETEADIYLINR
                     ENVTWLVEYYKTKWPFTFVVIDELSSFKSSKSKRFRALRKVRPKVQRLVGLTGTPAPN
                     SLIDLWPQIYLMDRGDRLETSQTRFKDKYFVPDKRNGPIIYSWALRDGAEAEIYNKIE
                     DICVSMKAKDYLKLPPRTDNVVSVKLSNMKAYKQFEADLVLEFKNKEISAANSAVLAN
                     KLLQMANGAIYDDDKATVAIHDDKLDALENIVEESQGQPILVFYQYQHDLERLKKRFP
                     QAEELTSVDKWNSEKIPILLCHPQSAGHGLNLQKGGHIIVWFGLTWSLEYYQQANARL
                     DRQGQTEPVIVHHIVAENTVDEKVLRILQGKEKNQNALLEAVKAQLGV"
     gene            10982..11152
                     /locus_tag="VBC94_000070"
     CDS             10982..11152
                     /locus_tag="VBC94_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978382.1"
                     /translation="MKKEYVVRIYTGREKNLEAKPQFEEKTFTRKADMLKFWNSCEAT
                     VKEKYTREREND"
     gene            11434..11628
                     /locus_tag="VBC94_000071"
     CDS             11434..11628
                     /locus_tag="VBC94_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978383.1"
                     /translation="MKIEFFLKQGPTKSVSNYGEVGIDEVIGAIKNNDIVALNGKNNA
                     VIIPKQNISHVIIHKEVTNE"
     gene            11621..12037
                     /locus_tag="VBC94_000072"
     CDS             11621..12037
                     /locus_tag="VBC94_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF722 domain-containing protein"
                     /protein_id="HEO6978384.1"
                     /translation="MNKLSNAQLKAFDEWLFDYRFIDNKIALRKLELQTDMNTEVDKN
                     IGGGRANFVSKVTEDVVARWDSDRELKGLSNFKEAVIATLNLLDEELTDIFNLRWGVG
                     SSNTWEEIAYTRHLSRKSIYRKRERILEIFSKKIGM"
     gene            12127..12579
                     /locus_tag="VBC94_000073"
     CDS             12127..12579
                     /locus_tag="VBC94_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEO6978385.1"
                     /translation="MLGGDGKLSKLTLKQKRFADEYIISANATAAAIKAGYSKKTARS
                     IGQENLTKPDIKAYIDERLEKLESEKIATQEEVLQYLTSIMRGDQQEKTLISVGEFGQ
                     KIVDIDVGAKDRIKAAELLGKRYRLFTDKVEMDVSSDITISVGEWDDD"
     gene            12569..13846
                     /locus_tag="VBC94_000074"
     CDS             12569..13846
                     /locus_tag="VBC94_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEO6978386.1"
                     /translation="MTIKQRPKINIVIKHPSKVFNKHIYDKLYNYSNFTEVHYGGASS
                     GKSHGVFQKIILKALNPKFKHPRKILVLRKVGATVRDSVFADIMSNLSYFGVLDKCKI
                     NMSAFRITLPNGSEFIFKGMDNPEKIKSIKGISDVVMEEASEFTLDDYTQLTLRLRDK
                     KHLEKQVYLMFNPVSKANWVYKAFFIKSPKNTVVYQTTYKDNRFLDDVTRENIEELAD
                     RNEAYYKIYALGQFATLDKLIFPKYDKQILNKDKLSHLPSFFGLDYGFINDPSAFLHV
                     KIDDANKKLYILEEYVRKNLTNDKIANAIKDLGYAKEEIRGDSAEKKSNQELRNLGIP
                     RMIDVTKGPGTVMQGIQYLLQYDWIVDERCVKTIEELENYTWKKDKKTNEYINEPVDS
                     YNHCIDAIRYAVQDRIYQSADRSKRMKNAKYYF"
     gene            13862..15394
                     /locus_tag="VBC94_000075"
     CDS             13862..15394
                     /locus_tag="VBC94_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO6978387.1"
                     /translation="MEEKQFLVGTRFNENANKQFIMLQEDFEAIDYESKLWIDQLKNY
                     ISRFKAEQLERLKELKRYYLGDNNIKYRPAKTDKYAADNRIASDFAKYITVFEQGYML
                     GVPVEYKNENKDLQAAIDLMSVRNNEDYHNVKIKTDLSIYGRAYELLTVEEIDDKKTE
                     VKLYQLPAEQTFVIYDDTYQRNSLMAVHFYDIDYGSGKRKQIIKVYTSDTIYTYEDYN
                     FETKGMRLKEHEEHFFKGVPVNEYANNEERTGAYESVLDNIDAYDLSQSELANFQQDS
                     VNALLVIAGNTYTGADENDYLDDGRLNPNGRLAISIGFKKAQVLILDDNPNPNGVKPQ
                     AYFLKKEYDTAGSEAYKNRLVADILRFTFTPDTQDMKFSGVQSGESMKYKLMASDNYR
                     EKQERLFKKGLMRRLRLAANIWAIKGNEATTYSLVNDTSIVFTPNLPQNDNEIVTAAQ
                     NLYGIVSDQTIFEILNTVTGVDAEAELERLKEEADKKQSLPEPRLVGDASGQEEPTAE
                     KP"
     gene            15354..16802
                     /locus_tag="VBC94_000076"
     CDS             15354..16802
                     /locus_tag="VBC94_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEO6978388.1"
                     /translation="MLVVKKNQQQKSLNYWQKRQEDILSYLDRTDLDVFSELQKLYNE
                     QAFELQKELFDFYTKYSEENKMTYQDVVKKLRHEDLSDYVANANKYRKQAEKDPELLK
                     RLNEQYVSARATRMDALNLELVYRAGILKGVLDSVFENHLKKAASYAYKKAMGGRSGT
                     INGPVLEELVRTPFDGYNYSEQLWGNTDNLVKNLQKKLKQGFVRGEHPRAIARDLAKQ
                     FNVANHRAETLIRTDGTMVINNATARRYLNAGLKYYRDLVRLDDRTTEICRKIAKENK
                     RKLLSELKPGINAAPYHFNCRTTIIPDEDELSIEVDPIDDKSTKYFKDVTSDWIDGNE
                     HKPQLSLLNEYVKNGTPYKVDGHNVVLDHSNYEYRVANWLSKKTGLQVDMVPRVNSPE
                     HINTPDYLVDGVPFDLKEITGSGKNVIDGNLRKAKKQATNIIFDITKTPLSFEEIMGQ
                     LEHIYMLDRRGLDISIIKNKDEVLAVLEKEGR"
     gene            16830..17018
                     /locus_tag="VBC94_000077"
     CDS             16830..17018
                     /locus_tag="VBC94_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978389.1"
                     /translation="MGVRPSSFFISIIHQPIKTIKEENMFIWQMVLVALGVLVLILIV
                     GIAAIAVKSIIAELKKVG"
     gene            17021..17287
                     /locus_tag="VBC94_000078"
     CDS             17021..17287
                     /locus_tag="VBC94_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978390.1"
                     /translation="MNKRIKKKRKLETAVVMLVAENAMQAEALRNQNKQITELRAIVQ
                     QNAQATNEEFASVKAATLDNQSAIADIAIDVNYIKKNYKRKWGK"
     gene            17443..18018
                     /locus_tag="VBC94_000079"
     CDS             17443..18018
                     /locus_tag="VBC94_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEO6978391.1"
                     /translation="MAEENVTTETTEQVDTQQETVEHPKQEHERTFTRADISKMMTAE
                     RAKWEAEQSEAIEKARTEGERLAKLSKDERAKEEEQKRLSAIAEREKAVAEREMRIET
                     QSLLAEKGLPLDFIDVVLATTADEVKTNIDNLQSIFDQAVENRVNERLTQKSPRTGNG
                     SVGMTKAEIMAIENDDERLKAIAANRNLFTR"
     gene            18037..18921
                     /locus_tag="VBC94_000080"
     CDS             18037..18921
                     /locus_tag="VBC94_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEO6978392.1"
                     /translation="MAENNLNVMSDLGEIKSIDFVNKFSTNINELLKLLGVTRKQELT
                     NDLKIQTYKWSTTLDKTNPAEGETIPLSKVTRAKAKEYTVEWFKKRRATTAEAIARHG
                     VARAINEADTRIMREIQNGFKTNFFEFLKKSTTKVTGTTLQSALSQSWAKIATFNEFD
                     GSPIISFVSPLDVAKYLGDTNVGADASNVFGMTLLKNFLGMQNVIVLPSVPEGKVYTT
                     AVENLVFAYLNVNSGDLGGMFADFTDETGLIGAARTRQLSNLTYESVFFGANVLFAEI
                     PEGVVEATIEAPKVVPGG"
     gene            18925..19281
                     /locus_tag="VBC94_000081"
     CDS             18925..19281
                     /locus_tag="VBC94_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEO6978393.1"
                     /translation="MDKELLLTEVKLFKGISAKDTSQDDLIKLSINESIDRILAKLND
                     YSDTEMTEIPKRLTFIVRDVAIKRYNRLNSEGTVEDSEEGKTFKWDSYLKEYESTLRS
                     AAIGKVYSGKGVARFI"
     gene            19292..19570
                     /locus_tag="VBC94_000082"
     CDS             19292..19570
                     /locus_tag="VBC94_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978394.1"
                     /translation="MIYKDRVILVYVDEQDDFLDKRTVEKPSGKIPCMENTFTKSEQM
                     GLFGKYDLNAFKLHLQGRYDGFSKIIYKGKPRLIKGLAHHKNSTVIYV"
     gene            19567..19911
                     /locus_tag="VBC94_000083"
     CDS             19567..19911
                     /locus_tag="VBC94_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO6978395.1"
                     /translation="MSLIYRMRGLDRFLRSVERKQKSVRIAVDKELSKSAARIERQAK
                     ILAPVDTGWLRAQIYSEQQRLLHYRVVSPALYSIYLELGTRKMEAQPFLDPALRKEWP
                     VLMANLKKMFKR"
     gene            19915..20274
                     /locus_tag="VBC94_000084"
     CDS             19915..20274
                     /locus_tag="VBC94_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978396.1"
                     /translation="MDYSPETLYLKKVKNRLGVLDIPIYFKLPKSDVLEPFIVVGTNI
                     SDLSKTAQTGAVIDDFSLNIDAFLPGDSRLDAEEIKSRMLRLLGRNNQIKAQILVDNS
                     IGREVYRVAINITEILI"
     gene            20286..20918
                     /locus_tag="VBC94_000085"
     CDS             20286..20918
                     /locus_tag="VBC94_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEO6978397.1"
                     /translation="METTVEITTAKPIKGKNIFYFIQSVDAELGSKAILPAYRTDGTT
                     KLGGDFIDEQTQQGRVLEKATDEHSIDLTSYYTPKDKAIEIVEIAKTTGKSVKVWRVI
                     ADESLKEKGSGTLEGKEVYPAKFGYGKIDEIEYSEGVEKLVEASYTLNIIDSLKDGKF
                     PLTAEDIALLTSVYAYQNPGETTGDYDNIQKKEVSGSLLGAVPGISSEGH"
     gene            20969..21424
                     /locus_tag="VBC94_000086"
     CDS             20969..21424
                     /locus_tag="VBC94_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978398.1"
                     /translation="MELTVAKRSIEIKFDFKTMFKINNRLGTVNKETGERNADGVGTL
                     FYKILERDDSAVVDLVKLSVGSGKKALSEDEVLDSIAELVEEEGSTEALFKEIENDMV
                     ESGFFKEKISKYIESMEKSVKYLEAREDADEAQIQIVKDMIGKMKDAIS"
     gene            21499..21729
                     /locus_tag="VBC94_000087"
     CDS             21499..21729
                     /locus_tag="VBC94_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978399.1"
                     /translation="MEGLQYKQIAERENLSELALNLRYTLNAKNVNANKLKLDKQRNK
                     IKRTYQNKQDLTNVSNDFAERVAKLNLHFQNL"
     gene            21748..25974
                     /locus_tag="VBC94_000088"
     CDS             21748..25974
                     /locus_tag="VBC94_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEO6978400.1"
                     /translation="MAGTFDGSIFADIGANTKDYEAAMARIVSTTQNAFRKAQDTAVN
                     NSNKMIQMIGQIMAQLANNGQSLGQRLGNAYSTGLKLSLGQIQRIAASIGEKIPEPIK
                     NGFNKAFSIIQNSVQKLSSAIPQPIKSAFTSATSAVVSFSSKITSAVSAAFNSVSSKA
                     STVADKISSSFGGRITSAVTNLATKLSTGLSNGFSRMSSSATSALNGISQRFASASSA
                     GEKLRSTVGSIISAFSLMAIARKGIQAITGAMDGAISRVDTMNRFPKTMALFGYSAQQ
                     SKASIDKLSKGIEGLPTPLDSAVKSAQQLAITTGSLDKGTELALAFNNAMIGYGATTE
                     GAEQALRQFNQSLGSGKIYAEEFNSVSEAAPGLMSKMAESFGFGKNGVQDLKAALSDG
                     SITAQEFADKMIELNKAQGGFAEMAQSSAGGIRTAWKNVHTAVVKGVAGMISAFDEAA
                     KANGMKTIAESINSIKPAINSAFATVNSIMPNLVAAFARISKMVNIDFSPLTSGIKSA
                     FGLLNTALGEFAKTGEVSSATIDRIKSKIASIGPTAIAAWALLNPASAIATIMPLISI
                     IGKIGVALGTVGSALGSFGEMVASGLSTASGLVGAFGRAMSGLPGIFGVAASRGLAVL
                     DGMTTAISSAAILAMSAIGPAAILGLVVVGLGLVNNQFGTQIDKLINTAITKGPGIIQ
                     GLVKGITSKIPALIASGTQLIAKFANALTVLLPVIIQAGVQLITSLVQGIGQNAGSLI
                     SSALKVIGTFVSSIASALPQILAVGVQLILNLARGIVQNIPLIIQYAQQIISNFGSSL
                     QANMPSIISNGIAISTNLNAGIIQMLPAVIQIATQVITGFISGIAQYLPQVIQGGIQI
                     IIMLVQGIIQNLPQVIQAAMQIIQSLISGLSQALPQIISAGIQLVVQLAVAIIKGLPQ
                     IISAGIQLIAGLGKAMLEAIPNALSGVWEGIKGGFSSMWDQITGKSSTSAAKVTSDMS
                     TMTMNVGMQTTTMANQTNIDTTTMLNSISQNTGLANLNATTNATQMATNVNAQTASMA
                     NQGLLNAAGFNEALSSNMTLANTNATNQANQMTANVNNATSQMSLGSLPNFLELSQQA
                     TSNMQTAQANATTSANGMSSGVTGATAGMASGVANNMLGITSTVSTGMNQAANAAKSG
                     GANISNSVNSSFTQAKSTVSSSMSGMNSTVTSGMNSINNTVKSGGNNIKSTFTSSFNS
                     AKSTVSNAMSSIRSSISSGMNSAVSVTRSAMNSVVSAMNAAASGARSAGYNTGIGFAN
                     GLASTAGTIMAIANSIAANVAATIRSAMDIHSPSRVTRELGAYTGEGFALGMQDWMDD
                     INAIGKEYANAVTDQRWETDSQLATSANISSAGVSSSLDNLSDEVRNQQLTSPTFEVH
                     NELVGDEIYTTIKSKEARESNKDNYFNFAT"
     gene            25987..26829
                     /locus_tag="VBC94_000089"
     CDS             25987..26829
                     /locus_tag="VBC94_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEO6978401.1"
                     /translation="MDLLVQKSDQSFRFSEIGLRILDVDDRSSSLEVDSRTVKGRSGK
                     IFASAKYGTKKVKVNGRLVVASIKDFMTKKDDVNGLLVDSEPFYITKMYPQKENLYDF
                     ELPGMKAGDLDLLNQSYTAWHYRWKVYVSSEPEYTFVGKSSEGLKYNFTIAFETAELP
                     FGETIAQNIVITNGTFNYSGTAPFSQLEYPWSVELTSTGGQSSFYLEIDGRRWAYQYS
                     APLNQGDVIVITGISTTLNKINVTARTNHEYFVLKQKVNKTILYSTNFQGTIKFLNFK
                     ELYK"
     gene            26842..30669
                     /locus_tag="VBC94_000090"
     CDS             26842..30669
                     /locus_tag="VBC94_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6978402.1"
                     /translation="MTTFLDHKDNEFEAQAVVKTTNAVNGERSLSGTIYTNEEVLNKV
                     DKGWKLEFDDEYYKIIYAKPTDTGNKIQVEFDAVHQFFYDFDKSSLHKQLNDGSHTFQ
                     AYLNFIFDGSGYTYSLEVVVKSFEKQSFGYKSRLDLFNDIIKSAGVEFFVRGKVVRIL
                     QKTGTDLSTIVRKNFNMNEIIIEKKINDFITYQKGFGAWADQEDHSKGRLEVEYESPL
                     AKEYGRIEGEPVVDERYTNADNLKSVLKQNVDSSYNIAVKIDIEDLTKAGYQYTQPIA
                     GDYIMAINETLGFKEKIRIVSFESEYDVTGQLINHKVTCNDIGTIKKQTVAVSQLSSK
                     INNNQAILDNAVFKANQALASADGKNTNYYGTEMPVDNPKGTLRKGDLLFLTVGDTTR
                     MYFWNGAEWIINSFSDDIDFVKKEITNKIIEVNAAMKLADEQLTEKYNAIVAKNVSQD
                     ELINQAKTLSDSAKKEATEALTNLISESKKLTDQMSALSKTEVEHYSTTNQQLTQIDG
                     TVKGLQTSYEALLKKDGDITQALANYKQTIDQNSASIMTNKKTADNALSSLQTQVIQT
                     SNEITTRLSQTNIEALITSKSAVIADNKVKETADSFSRELTRVNNTIDNLKIGTVNLL
                     SGTKGWTKNWFNRNNWQADDDSLSIGNFNLTVLKRKMMWSGISQSYAVKSGESYVFSA
                     YAKSSIDNDVVNLYLLPNDQSQQAKISISFKSITLNTDWQRIVIKFNVNSDGYILPRF
                     ERFNENGWLYIAGYQLEHGNVTSDYSESPADQEDKMTVEFNKINDTVNSHSQLIGKQN
                     ESLTATIQKVDNIQSTVSNVDGRLSRVTQTADGIVATVSQLDNNILPGTKKFTGWQTE
                     GAVLEELTQSPYPFVFKKWVSGNKVSPLIEYDVKKDQEYTFTAYISREQAGNLYFYLY
                     DLWNHHITSTTPRETIIKDVTQTIRRFKITFIPTRDGKIRPRFAMLASDPGSFSTGGF
                     MLSKGTVELEWSESKQDIKDSVTAINTTVTQTANGWAIKNLTSSGTVLSQLNLTDGNV
                     KLEGKLIHLSGQSIIDNGIITNAMIKDLTADKITAGTIDANRINVINLDASNITSGRM
                     SADLIRSGVLISQNGAMLADLNTGQIEFYTDSPAIKRITAGYPNQFVKFATGNVEGKG
                     AAGVTVIGSNRWGSESSNDGGFVGIRAWNGKDIDTLDLVGDSIHLASSAYTNADGWEI
                     ITLPNQLSIDARNINHRVTSKIKVGDIWLWKNTSTYTSMKDTINMIIDNLQLLHNNKT
                     TEKGYSYTIPGKI"
     gene            30678..30830
                     /locus_tag="VBC94_000091"
     CDS             30678..30830
                     /locus_tag="VBC94_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978403.1"
                     /translation="MEEKIKSNLALQIAQLSLDKATLQAQNEQLQLQIAELNQQLDEI
                     TKPGAK"
     gene            30841..31257
                     /locus_tag="VBC94_000092"
     CDS             30841..31257
                     /locus_tag="VBC94_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEO6978404.1"
                     /translation="MKQWNIVGKPFPIYQDGVVLKTDVRLQGDDGTYLPQELIGNHVD
                     KDNQSLIKLVLETFAKENVVDFAMLESVKDIEQLKADKVTLIKKIAEADEAISKMSQA
                     TKDMQLKVDKAVVELTDLVTSSVSSLSVPSTSMESS"
     gene            31320..31481
                     /locus_tag="VBC94_000093"
     CDS             31320..31481
                     /locus_tag="VBC94_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978405.1"
                     /translation="MTYTFKKDHLIVDVWYRRVANGICDFEAVPKLFNLRDVVLELLS
                     QKVTDKESE"
     gene            31491..31781
                     /locus_tag="VBC94_000094"
     CDS             31491..31781
                     /locus_tag="VBC94_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978406.1"
                     /translation="MIDFVQVGAFAGAALSILGVWGFIVNPFKKAMEANEIAMAQLKD
                     SLKELAYELKNIDRDREITKAVIDRHEERLGRVEDDIIINKEQIKTLFNKEK"
     gene            31783..32034
                     /locus_tag="VBC94_000095"
     CDS             31783..32034
                     /locus_tag="VBC94_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO6978407.1"
                     /translation="MINWKVRIKNKAFWSAIIPAIFLVVQAVANVFGYTLELSDLGNK
                     LLVVVNSGFSVLVIAGIVTDPTTQGVSDSSRALSYTEPK"
     gene            32164..33507
                     /locus_tag="VBC94_000096"
     CDS             32164..33507
                     /locus_tag="VBC94_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEO6978408.1"
                     /translation="MATYQEYKSRSNGNAYDIDGSFGAQCWDGYADYCGFLGVPYANC
                     TNTGYARDIWEQRHENGILNYFGEVETMQAGDVAIFMVVDGVTPYSHVAIFDSDAGGG
                     YGWFLGQNQGGPNGAYNLVKIPYSATYPTAFRPKVFKNAVTVTGNTGLNKGDYFIDVS
                     AYQQADLTTTCQQAGTTKTIIKVSESIAWLSDRHQQQANTSDPIGYYHFGRFGGDSAL
                     AQREADLFLSNLPSKKVSYLVIDYEDSASADKQANTNAVIAFMDKIASAGYKPVYYSY
                     KPFTLNNVDYQQIIAKYPNSIWIAGYPDYEVRSEPLWDYFPSMDGVRWWQFTSVGVAG
                     GLDKNIVLLADDSSKVDIPKIDKPQEPQSQLTFNQKLDTNTKLDNSNVPYYEATLSTD
                     YYVESKPNASSTDKEFIKAGTRVRVYEKINGWSRINAPQSEQWVEDAYLIDATDM"
     gene            34458..34970
                     /locus_tag="VBC94_000097"
     CDS             34458..34970
                     /locus_tag="VBC94_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978409.1"
                     /translation="MIVDLKEIVNDYELNFCGKRCKVETNFKHLPEFMILFDIRDLYH
                     LLGIHKLKTKYRATNWVEAVKADVFLLSNYSKHPNFREVLPRVDNYNFLYEIFYQFRV
                     NVCILDKDLTKNTMKLSVVFYKDNKKKLVVVGLKRDETGVFRPATLHESRNNPYKRIR
                     HTAIKSITWI"
     gene            35198..35407
                     /locus_tag="VBC94_000098"
     CDS             35198..35407
                     /locus_tag="VBC94_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000965644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO6978410.1"
                     /translation="MLYIDEFKEAIDKGYISGDTVAIVRKNGKIFDYVLPHEKVRDDE
                     VVAVESVEDVLAELGNVRCDISSCQ"
     gene            35783..35980
                     /locus_tag="VBC94_000099"
     CDS             35783..35980
                     /locus_tag="VBC94_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978411.1"
                     /translation="MSEKAILLIILIAFVIIAIIKIALLKLYMNTIKPKVDNEGNIIT
                     KPVKDDLTPEEREEREFDKDW"
     gene            complement(36024..36956)
                     /locus_tag="VBC94_000100"
     CDS             complement(36024..36956)
                     /locus_tag="VBC94_000100"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEO6978412.1"
                     /translation="MVSLKTEIAGFSFDNCLMNAAGIYCMTKEELLAIENSEAGSFVT
                     KTGTLKAREGNPQPRYADTDWGSINSMGLPNKGIDYYLDFVTELQDQDNSKNHVLSLV
                     GLSPEETHIILKKVENSSYNGLIELNLSCPNVPGKPQIAYDFEMTDLILSEIFSYYQK
                     PLGIKLPPYFDIVHFDQAATIFNKYPLAFINCVNSIGNGLVIDDETVVIKPKNGFGGI
                     GGDFIKPTALANVHAFYKRLNPSIKIIGTGGVKNGRDAFEHILCGASMVQIGTALQKE
                     GPEIFQRVSRELKEIMADKGYQSLEDFRGQLNYL"
     gene            complement(37144..38379)
                     /locus_tag="VBC94_000101"
     CDS             complement(37144..38379)
                     /locus_tag="VBC94_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6978413.1"
                     /translation="MALKELTAKEFESYSGNYDLQSFMQTPEMAKLLKKRGYDITYMG
                     YQIDGKMEIISIVYTIPMTGGLHMEVNSGPAHSNSKYLKHFYKELQNYAKSQGALELL
                     IKPYDTYQEFTGEGEPKGAPNTYLIDGLTSIGYHHDGLHIGYPGGEPDWHYVKNLEGI
                     TPQNLLKSFSKKGRPLVKKAMSFGIKIRVLKREELHIFKDITSSTSDRRDYMDKSLDY
                     YQDFYDSFGDKAEFVIATLNFREYDHNLQLNAKKLEEQITVLDNRHQNNTDSAKYHRQ
                     RTELVNQLASLDKRRKEVEPFIQKFGNQDVVLAGSLFIYSPKETVYLFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRQSTFYNFLGIQGNFDGSDGVLRFKQNFNGYIVRKMGTFRYY
                     PNPLKYKSIQLLKKILRRT"
     gene            complement(38398..39609)
                     /locus_tag="VBC94_000102"
     CDS             complement(38398..39609)
                     /locus_tag="VBC94_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6978414.1"
                     /translation="MYREITAVEHDRFVSESNQTNLLQSSNWPKVKDNWGSQLLGFFD
                     GETQIASASILIKSLPLGFSMLYIPRGPIMDYSNLDIVTKVLKDLKAFGKKQRALFIK
                     CDPLIYLKMVNAKDFENSPDEKEGLIAIDHLQRAGADWTGRTTDLAHTIQPRFQANLY
                     ANQFGLDKMSKKTRQAIRTSKNKGVDIQFGSHELLEDFAELMKKTEDRKGINLRGIDY
                     YQKLLDTYPNNSYITMASLDVAKRLEKIEKECQIAQSERIKSLELNREKKVKQHQDTI
                     DRLNKEIDFLKEAQKAYDRDIVPLAATLTLEFGNTSENIYAGMDDYFKSYSAPIYTWF
                     ETAQRAFERGNIWQNMGGIENDLSGGLYHFKSKFEPIIEEFIGEFNIPVNRLLYKASN
                     YVYALRKKRNS"
     gene            complement(39622..40852)
                     /locus_tag="VBC94_000103"
                     /pseudo
     CDS             complement(39622..40852)
                     /locus_tag="VBC94_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
     gene            complement(40837..41649)
                     /gene="yidA"
                     /locus_tag="VBC94_000104"
     CDS             complement(40837..41649)
                     /gene="yidA"
                     /locus_tag="VBC94_000104"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEO6978415.1"
                     /translation="MTSIKLVAVDIDGTLLNSKREITPEVAKAVQEAKSKDVKIVIAT
                     GRPIIGVQDLLEELKLNEEGDYVITFNGGLVQDTATGDDIIKETLTYEDYLDFELLAR
                     KLGVHMHAITKEGIYTANRDIGKYTIHEATLVNMPLFYRTPEEMGDKEIIKLMMIDQP
                     DILDAAIAKIPKKVLDNYTIVKSTPFYLEILPKNVNKGTALLHLAEKMGLTVDQTMAI
                     GDEENDRAMLEVVGNPVVMQNGNPELKKIAKYITKSNEESGVAYALREWVIN"
     gene            complement(41721..43037)
                     /locus_tag="VBC94_000105"
     CDS             complement(41721..43037)
                     /locus_tag="VBC94_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6978416.1"
                     /translation="MNEKVFRDPVHTYIHVNNQVIYDLINTKEFQRLRRIKQTSTTSF
                     TFHGAEHSRFSHCLGVYELARKVTEIFDEHYSDLWNKNESLLTMAAALLHDIGHGAYS
                     HTFERLFNTDHEAYTQEIITNPTTEINAILRKVAPDFPDKVASVINHSYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYYTAASYGQFDLTRILRVIRPTDSGIAFARNGMHAVEDYIVS
                     RFQMYMQVYFHPASRAMELLLQNLLKRARFLFDTHRDFFEQTSPNLIPFFTDQYDLQD
                     YLALDDGVMNTYFQSWMQADDNILADLANRFINRKVFKSITFEEADKENLVKMKELVS
                     QVGFDPDYYTGVHANFDLPYDVYRPEHSNPRTEIQIIQKNGQLAELSSLSPIVKALTG
                     SNYGDQRFYFPKEMLTLDSLFSSTKEEFQSYITNEHLTLTKDNSHS"
     gene            43101..43499
                     /locus_tag="VBC94_000106"
     CDS             43101..43499
                     /locus_tag="VBC94_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEO6978417.1"
                     /translation="MREVMKLEINNNIQIDNETEMIHEIHDCQFIEKGSYVYLNYINA
                     EGERVVIKANHEELLMTRFSNPKSVMRFHRETPALVNIPTPLGIQHLITETSHYQFDL
                     SQQRLHINYVLKQTETGACFANYELKIQWY"
     gene            43843..46527
                     /locus_tag="VBC94_000107"
     CDS             43843..46527
                     /locus_tag="VBC94_000107"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HEO6978418.1"
                     /translation="MLKEQKKSLFYTQGQEEVLTSLESSREGLSTTEAKNRLEMYGRN
                     ELEEGKKRSLIAKFFDQFKDLMIIILLVAAALSVITEGMHGLTDALIILAVVILNAAF
                     GVYQEGQAEAAIEALKDMSSPIARVRRDGHTIEVDSKELVPGDLVMLEAGDVVPADLR
                     LLEAASLKIEEAALTGESVPVEKDISQVVAEDAGIGDRVNMAYQNSNVTYGRGYGVVT
                     NTGMYTEVGKIADMLANADESETPLKQSLVQLSKLLTYLIVIIAVITFLVGIFVRKEG
                     WIEGLMTSVALAVAAIPEGLPAIVTIVLSMGTKTLAKRNSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGVLQSSSEEISVDNNTLRIMNFSNDTKIDPSGKLIGDP
                     TETALVQFGLDKNFDVREVLKNEPRVAELPFDSDRKLMSTIHKESDGRYFIAVKGAPD
                     QLLKRVTKIEDNGLVRDITAEDKEAILNTNKELAKQALRVLMMAYKYETQIPTLETDI
                     VESDLVFSGLVGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGIIDA
                     NDTEDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNDGKVVAMTGD
                     GVNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKS
                     IQYLLSANMAEVFTIFFATLLGWDVLAPVHLLWINLVTDTLPAIALGVEPAEPGVMTH
                     KPRGRQSNFFDGGVMGAIIYQGILQTILVLGVYGWALMYPEHAGYRMIHADALTMAFA
                     TLGLIQLVHAFNVKSVYQSIFTVGAFKNRTFNWSIPVAFILLMVTIVVPGFNKLFHVT
                     HLSSTQWLTVVIGSLLMVVLTEIVKFIQRKLGQDEKAI"
     gene            complement(46572..47432)
                     /locus_tag="VBC94_000108"
                     /pseudo
     CDS             complement(46572..47432)
                     /locus_tag="VBC94_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163483.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            47584..49515
                     /locus_tag="VBC94_000109"
     CDS             47584..49515
                     /locus_tag="VBC94_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEO6978419.1"
                     /translation="MSNFYKLLKEKFPRKEDIVTEMINLEAICQLPKGTEYFISDLHG
                     EYDAVDYLLRTGAGSIRAKLLDCFDWQKIVAVDLDDFCILLYYPKEKLAFDKMNLSVS
                     AYKTKLWEMIPLQIQVLKYFSSKYTKSKVRKQLPGKFAYIIEELLAEIDRNPEKKSYF
                     DTIIEKLFELDQVEDLIIVLSQTIQVLIIDHLHVVGDIYDRGRYPDRILNRLMAFPNL
                     DIQWGNHDVTWMGAASGSYLCMVNVIRIAARYNNITLIEDRYGINLRRLVDYSRRYYE
                     PLPSFAPILDGEEMTHPDELDLLNMIQQATAILQFKLEAQLIDRRPEFQMHNRQLINQ
                     VNYKDLSISIKGVVHQLKDFNSRCIDSKNPSRLTSEEEELLQQLMIAFQTSESLKKHI
                     DFLFEKGSMYLTYNDNLLFHGCIPMHSNGDFKSFKIAGKTYGGRDLLDLFESQIRLAY
                     ARPEKHDDLATDIVWYLWCGENSSLFGKNAMTTFERYYVSDKVTHQERKNPYFKLRDK
                     DDICTALLQEFDLPKFGHIVNGHTPVKEKNGEQPIKANGKMLVIDGGFAKGYQKNTGL
                     AGYTLIYNSYGIQLISHLPFTSIEEVLSGTNYIIDTKRLVEEAKDRILVKDTTIGQKL
                     TKEIKDLDHLYRHFQEYDD"
     gene            49605..50729
                     /gene="queG"
                     /locus_tag="VBC94_000110"
     CDS             49605..50729
                     /gene="queG"
                     /locus_tag="VBC94_000110"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEO6978420.1"
                     /translation="MDIKAEIQKLAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRIYPERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPNSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNPLATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILEFMSNLTLKDEDSRKELELIRHKWQF"
     gene            50798..51896
                     /gene="prfB"
                     /locus_tag="VBC94_000111"
     CDS             join(50798..50869,50871..51896)
                     /gene="prfB"
                     /locus_tag="VBC94_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEO6978421.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENQMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            51915..52607
                     /gene="ftsE"
                     /locus_tag="VBC94_000112"
     CDS             51915..52607
                     /gene="ftsE"
                     /locus_tag="VBC94_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEO6978422.1"
                     /translation="MALIEMSGVTKKYRRSTTALRNLNLSIQQGEFVYLVGPSGAGKS
                     SLIRLLYREEKLSSGRLKVGEFNLNKLKRRQIPILRRSIGVVFQDYKLLPTKTVYENV
                     AFAMQVIGAKRRHIKKRVPEVLELVGLKHKMRSFPTQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEIAWEIMHLLERINLQGTTVLMATHNSQIVNTLRHRVIEIEAGSVI
                     RDEEKGEYGYHD"
     gene            52591..53520
                     /locus_tag="VBC94_000113"
     CDS             52591..53520
                     /locus_tag="VBC94_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6978423.1"
                     /translation="MVIMINFFRHFWESLKNLKRNFWMTFASVTSVTITLLLVGLFSS
                     VLLNVEKLTTDVSGNFTISAFLNVDSTDAQKQVKDKDGKLKDNPDYHKVYDKIKRISG
                     VEKVTYSSKAEQLKEVQKEYGSDVIDDTYKDALLDVYVVGTSSAKVSKSVSEAIGRIE
                     GVDYTKEPIDSTKLSNLTDNIRIWGFGGVALLIVLAIFLISNTIRMSIMSRRTDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGILGAIVPSLIFYFGYQFVFNKFNPKFETSHVSLYPM
                     DIMVPAIIGGMVIIGIIIGSLGSVLSMRRYLKI"
BASE COUNT        17985 a         8738 c        11083 g        15731 t
ORIGIN      
        1 tgctttcatt ttagaatatt ctctaaaata tgtcaataaa aaacgcaaaa aaatagttgt
       61 ttcttattta caattttaaa taaaggagga agaaagatgc ctgatattgc aaatggacgc
      121 aaaaaaattt gtgacttctt aaaagaaaac aatatcaaaa aagcagcttt agcagctgca
      181 tatggcatga gtcgacaaga agttactaat attttaagtg gctcaactcg tggcctgaaa
      241 gctaatcaat ttatcttacg agttatcgaa gattataaaa ttgattaaat cgcaggaaga
      301 gtttgaagaa agggataatg tatgaacgaa atttttaatt ttcacggaca agaagtccgc
      361 acagtaacaa tcaataatga agcatacttt gttggtaaag atgtggcaga cattcttggt
      421 tactcaaacg ggagccgaga tgtaaataga cacgttgaag aagaagatag gctaaagtac
      481 cgttttggta cctcaggtca agatagagaa atgactatca tcaatgaatc aggtctttac
      541 tcacttatct tatctagcaa gttaccgcaa gcaaaagatt ttaaacgttg ggttacgtca
      601 gaagtcttac cgacaatccg taaacatgga ttgtacgcta ttgatgatct gctggaaaat
      661 ccagacatgg caatcgcagc acttcagaag ctcaaggaag aacgtcagct acgtttacag
      721 gcacaagaag aagtagctca aaagaatcaa attattcagg aattacaacc aaaagcaact
      781 tattatgatt tgattttaca aagtgaatcc ttagtggcta tctctgtcat tgcaaaagat
      841 tacggcatga gtgctaagaa actaaatagt ctgttgcatg aattgaaagt tcaatataag
      901 caaggcaata cttggctact atatcagaag tatgctagca agggttacac acaatccaag
      961 acgcatccga ttgatgcaga acgcagtaag atgcacactt actggacaca aaaaggacgc
     1021 ctgttcatct atgacttgct aaaaaacaaa aaggggattt tgccattgat tgagcaggaa
     1081 gaagtggcgt aataaaaaaa gcacctaacg aagtcaggcg ctcaacaaaa ataactaact
     1141 agattataac acagaaaaga gagtttaaca tgccaaaagc agaaattatt tacagaccag
     1201 ctaatcagtc tgaaaaggca acgcatggag actatgccca tctttgccag gtttgggaag
     1261 gtttgacaat cggaacggcc aaggtttggg cggcagaaat gcgagaacat ccagacttta
     1321 ggcagtttat tgacaatcca acgcaccgta ttgtctttat tgattacgag ggtttccgtc
     1381 tattcgtcaa atggaagagt cgcaatcggt acagaccgaa aaaagaaaca ttgtctgaaa
     1441 tgatagacaa cattaaacgc gaaaaacaat taggagtata acatcatgaa gaaattatta
     1501 aaaaaactat ttgtaaaaaa aaatcgaaaa atagatgtgc gtccagcatg gaagattgaa
     1561 acacatggct gggaagctaa tgcacgacgt tacgacaata tgattgaaca taatagaggg
     1621 cgtacatgtt aaaagaagta ttactagaaa atgaactgtt gcgtgatgaa aatagacgac
     1681 taactaatga aatggcagat tattatttta cgaacgtagc aaaagcaaat ttattggata
     1741 ttatcgtcaa tgaaggctac atcttagata gcacactgga aaaatgtatt gctaagctag
     1801 ataacgttga tagacaaata ctagaaaaaa attggagtga tgataatgat tgaagttaaa
     1861 agacgagata gcttacctat cctatctgct cagttgagtg ttatcaagca agatttaaaa
     1921 ttgatgcttt ggattgagct accgacgtta aatgacgtag agaaatatga agctctttat
     1981 caaggaaatg attttaaatt cagtgaaact tttttggagc gtcaagcgat gagaaaagcc
     2041 ttttggagac gattagctaa ggaaactttt aaagaagacc aggataggag gaactttgtt
     2101 aagttggctg agaaagaatt taaaggtgca actttgtcat ggcaagaatt tttgtggggt
     2161 aaaaagaaat gaatatttca ataaaactca taggtgaaac cctggctggt ttaaatgata
     2221 ttttacgccg gggggggggc tctcttgctc ccaaaaccaa gccttagcag acgcagtttt
     2281 tatattaaca actttaaaac aagtaattga ggagagaaaa tgaactactt agaatacgca
     2341 ctagcttatc ttgaacgtga gttagaaatt attgataacg aagctatcga agttgagttg
     2401 ccggacggag attgggagtt tgtgcttaat ccctattacg aggaaggttt acatgataac
     2461 ccatactatc gtagtcaggt tgctaaagac attcttgata ttaaaggact tttggggagg
     2521 tgaggctatt gattgcatca aaaaagacgg cttaggaaac cgtcaacaat tacacacaca
     2581 cttaaattat aacacagaaa gagagaaaca caatgattga agtaactttt aaagcagaga
     2641 gcatggcagg tgttttcgat gctatgcgtg aagctctgga tatgccgaag acagtaacca
     2701 aggatgttgt tgaagaaaaa ccagctccta aaaagaaaga agagtctgta actttaacat
     2761 tagctgatat taagaaaatg gctaaagcca aggttgaaga agggaagtca aaggacatca
     2821 aggaagtctt gagagagatg aacgtcgcaa aagttggtga acttgaagaa taccagtttg
     2881 cggagtttgt tgagaagttg gaggctctct aatgccagta gaaaatcacg cactactgtc
     2941 tgcttctagt gctcacagat ggctttattg ccctatgcta ccgagattag aagcagacta
     3001 tcctagccgc gacaccgtat acactcaaga gggcacaagc gcccacgagc tttctgaaat
     3061 caaactgatg tacaagtctg gtaaaatcac caagcgtaaa tttaacacgt taacaaaggc
     3121 ttttaaggaa aactcagact tctacaacga agagatggaa gagatgacgg agctttacac
     3181 agatattgtt atggagcacc taaatgctta tgagaatgcc gaaattgaac ttgaaaaacg
     3241 ggttgatttt agcgactggg tgcctggcgg ttttgggact tcggatgtcg tcattttggc
     3301 ggatggggtc attgaaatta ttgatcttaa gtatggcaag ggtatgcctg tgtctgctaa
     3361 ccaaaacccg cagatggggt tgtacgctct gggagcttat gcttcctacg atatggttta
     3421 tgactttgac cgtatcaaga tgaccattat tcagccacgt ttagattcgg ttagttctgt
     3481 tgacatttac gtagaggagc ttctgtattg ggcggataat gttgtcttgc ctatggcagc
     3541 tcaagcagat gcaggcattg gtgactggaa cctaagtgaa aaagtattgc agtggtctcc
     3601 tgtcgcagct aaattggtgc caagagcgca agaaaactgg gaattaattg ataaatatga
     3661 ctatcaggag cctgtctatt tatctgatga agccgtcgca gagatccttg acaaagcctc
     3721 agctatcaag aagtgggttg agtcagttga agcctatgcc ctgaaagaag cactctcagg
     3781 aaaagaagtt ccaggctata agattgtcga aggtagaagt aatcgagtta tcactgataa
     3841 agacaaagca attgatattt tacaagataa cggctttgac gatgagattt ttaaaccgaa
     3901 agagctgtta tcaatgggag ctcttgaaaa gttaataggt aaaaccactt ttgctgattt
     3961 attagcagaa gtaattgata aaccacaggg caaacctgta cttgtccctg aaaaggataa
     4021 gcgcccagca ataaacagtt tagaacaagc aattaaagat tttgaatagg agaaaaataa
     4081 ttatgacaac aacaccaaac acaactaaag tagtgaccgg aaaagtacgc ctaagctatg
     4141 tagctttact agagcctaaa gcctttgaag gccaagaggc taagtattca acagttatct
     4201 taattccaaa aacagacaaa gccacaatca agaaaattaa agacgcgcag aaagctgctt
     4261 atgaagctgc caaggacaat aaactcaaag gagttaaatg ggagcgcgtt aagacaacgc
     4321 ttcgtgatgg tgacgaagaa atggataccg aagagcaccc agagtacgca ggccatatgt
     4381 tcatgtcaat ctcaagcaaa actaaaccgc agatcattga caagtataaa aactctgttg
     4441 attccgcaga agaagtctac tctggcgtct atgctcgtgt atcacttaat gcctatgctt
     4501 acaacacagc gggaaataaa ggaatctctt gcggattaaa caacgtccaa attgttgcta
     4561 aaggagacta ccttagcggc cgttcgtcag ctgatgcaga ttttgacgag tggaacgaag
     4621 aagaggacga agacgacatt ttatagttga gggcctcttt agaggctcct catttttaaa
     4681 ggagaggtat gagacattta aatattgata ttgaaaccta tagttcgaat gacatcaaaa
     4741 atggggttta caagtatgct gacgcagaag attttgagat tttacttttc gcttactcta
     4801 tagatggcgg agaagtagag tgccttgatt tgacaaggca gtctctacct gaagacatca
     4861 aagatatgtt atttgatgat aaagtccgaa agcacgcctt taatgcccaa ttcgaaagag
     4921 tttgtctcag tcgttacctc ggcctacctt actatctaga tccttgccaa tggcaatgca
     4981 ccatggtgct ggcccaagag ttgggtctgc cttcaagctt ggaaaagtgt gcgctgtatt
     5041 taaaattagc acaggaaaaa gatacctcgg gtaaaaactt aatcagatac ttttccctgc
     5101 cttgcaaacc aagtaaaact aacggtggga gaactagaaa tttaccagaa cacgcccccg
     5161 aaaagtggca aatgtttatt gactactgca tccaagacgt tgttgttgag atggcaattg
     5221 ccgaaaaact agagtcagtt cctgtgcacg accgtgagtg ggattactac gtctgcgacc
     5281 agagaatcaa cgacagaggc gtggcgcttg ataaagagtt agttgcttcg gctttgtatt
     5341 gtaaagatgt taagatggaa agtttgtctg gtgaactaaa agctctaaca ggacttgata
     5401 atcctaatag cagggcgcag ctgctaccgt ggctaaaaga acacggttat tcggctaatg
     5461 ggctgactaa agcagatgtt gaacgggaac ttaagacggc cgaaggagaa cttaagagag
     5521 tcttagaact taaactacaa accgcgatgt caagtctaaa aaaatatgaa gctatggaaa
     5581 gagctatgtg ctcagacgga cgagttcatg gactacttca gttttacgga gctagcagga
     5641 caggaagatg ggcgggcaga gttgtccaag tacagaactt agctaggaat tatataaagg
     5701 atctagatga tgctagagag tatgttaaaa agcgtgatat tgatgctgtg gagattttat
     5761 acgatagtct taatgacact ttaaagcagc tagtaagaac ggcactcgtg gccaaagact
     5821 gctgtacctt ctacgtctct gacttctcag cgattgaggc tagggtgatt gcgtggtttg
     5881 ccggagagca gtggaggctt gacgtgtttt cgacgcacgg aaaaatctat gaggcatccg
     5941 ctagccagat gttcggaatt ccaattgagg agattgataa ggaactacgc caaaaaggca
     6001 aaatctcaga gttggcactt ggctatcaag gaggtcctgg agcgctcaag cagatggggg
     6061 ctctaaatat gggggtcaag gaagaggagc ttcaagggtt agttgatgac tggcgcagga
     6121 ccaataagaa aatcgtccaa ttttggaaag acgtacaaag agccgccatc aaagccatca
     6181 aatcgagagc accgataaaa cttggaaaac tacgatttag ataccgtaaa ggtttcctct
     6241 ttataacatt gcctagcggt aggaacttag cttatgcaag agccaaggtt gagcctggtg
     6301 actttggaga caaaatcatc tatgagggcc aaggagataa ggcatacttc acagcgcaag
     6361 agacttatgg cggtaagctt gtcgaaaata tcgttcaggc gacggctagg gatattctag
     6421 ctgaagcgct tctgaggatt gaagctgcag gccatggtgt tgttttccac gttcatgatg
     6481 aggctattat cgaaggctca ggcctgacaa tcgaagaggt taatgagttg atggctcaag
     6541 cgcctgactg ggcggagggt cttcctttaa atagcgaagg ctacataaca aagtattata
     6601 tgaaggatta gatagatgaa gcaagaaaaa ctaatagtaa agtcttctcc tctgcaagag
     6661 ctccatatcg caacgggtaa ttcacgaaca gctaagacgt ggaaaaatat tacgctaact
     6721 tggcaggagc tggttgagag gttagagaaa cctacagtca cccaagagac gtttgcggag
     6781 taccagaaga tgtctcgagc agaaaaaggg caagcaaaag acgtaggggg ctttgtcggt
     6841 ggatggctaa agcaaggtaa acggaaaaat gaaaacgttc aaagtaggtc cttggttgcg
     6901 cttgacgcag atagcccaag taaagatttc ttagataggc tagacctgct tgcggattat
     6961 gcctacgtac tctacagtac tcacagccac tcaaaaaaag ctgctaagta ccgtcttatt
     7021 atccctactg accgtttaat gatgcctgat gaatatgagc cagtcgctag atacttggct
     7081 aaccaactag gcatgtcaaa ctttgatgac acgacttatc aaagtgtacg cttgatgttc
     7141 tggccgagtc actcaagaga tgcagacttt acgtttaaat ataacgacga ggcttttctg
     7201 agtgttgatg aggtgcttga cacatacccg gactggcatg actcaagctt ctggccagaa
     7261 agcccgacgc acgctgttaa gagacagcgc gaagctaaaa aacaaggtga cccgcttagt
     7321 aaaaaaggac ttattggagc cttttgtcgt aactatgaca ttagacaggc cattgcaacg
     7381 ttcttacctg aggtttatga agaaggaaca actcctgata ggtacaccta cactgagggc
     7441 tcaaccgcaa acggcttagt tatctatgat gatgtcttcg cttatagtca tcacgggaca
     7501 gatcccgtgg gggatacgct tgtaaatgca tacgaccttg ttcgtatcca taaattcgga
     7561 gagcaagata gcggggctaa agataatact cctactaata agctaccatc aagcaaagcg
     7621 atgaatgctt ttgtctgtga cttacccgaa attaaagact atctaatggc ggaggcttta
     7681 ggcgatttcg atgaagagtt accagtcgaa gatgacagaa gctggcttga aattgatgag
     7741 aggggtgaac ctgaggtcaa tagttatttg ctagcaacgc agattattaa ggaggttccg
     7801 atttattggg acggctttga gttcttaaga tacgacgcta aaaaaggcat ctggttgcca
     7861 aatgcagagg aatatttgaa gagttatatc tcaactaaga aactcggtaa aattactaag
     7921 attaggcaca ttagcgaaac cattgtagcg attaaagcac aggctttttc aagcgaagtg
     7981 tttaccgaga gtgatcttaa caagatagtg ctagcgaacg gagtctatga cctgagggat
     8041 aacagcttta agactaagtt tgatccagaa ttgcatgcca ggtcaagcca tcccgttgtc
     8101 tatgaccccg aggcgacctg tgaaaccttt gagggttttc ttagggagac cgtcggagct
     8161 gaaaatatag atttcatctt tgagtggttt ggctataact tttatcgtga atacactatt
     8221 caaaaaatgc tattcatcta tggcagcggc ggtacgggta aatcaacact gattaatatt
     8281 ttacgtgaaa tgataggtgc tgataattat tcagccgtga cactgcagta cctgatgcaa
     8341 gaacgctttg caaaaatcgg cttatatcgt aagacagcca actttgacac tgacgcaaag
     8401 ccccaatatt tagcagatgg cgcaaccctt aaaatgttga cgggagagga tactatccac
     8461 gcagaccgta aaaataaaga gccgattaac ttttacaatt atgctaagct gtcttttgcc
     8521 atgaatgagc tcccgcctat gcgagatttc agcggaggaa ttaagcgccg catgatgatc
     8581 cttgagatgg ataaagtttt aacgcaggaa gttaaggcaa aataccccct agataagatt
     8641 atgggcgaag tgtctggtat ctttaacaga gcgatggagg ggcttagaaa ggccttaagt
     8701 aagagagatt tcagtattag tgccagcatg aggtcaagtg tcgagaaatg ggaaaaaggc
     8761 aacgatgtcg tagctatgtt ccttgaagac gagtgtgaac ctggtgaaga cttcaaagct
     8821 cctgttaggg acgtctaccc agcctataaa ttctattgtc aggattcagg ctacaaacct
     8881 ttggcaaaaa atgcatttaa ccaccggcta agagagctaa attatgaaaa taaaaacgtt
     8941 aaatctggtg gaaagcaagc caaaaattgg gtcggtttta ggctaaaaag tgagttttag
     9001 gggttacttt tttgggatta gagtttggta accctagaaa aaagttacca agttaccggt
     9061 ttttcaaact ttaaaaataa aaaatttaaa aataattttt aaaaaaccaa aactgaattg
     9121 gaaaaatcgg taacttagta actcgaccgt tagaaatgtg ttcgaaaagt aaccccggta
     9181 gctttttggt agccctaaga ggcttttcct agcaatagtt tagcccctat ggttaccaat
     9241 ctaccacttt tcttctatta acttaaatat aaatataaaa aataaatata tataaaataa
     9301 ggcatatata aaacacatat ataaaaaaga gaaagtaagt tgaaaaaaaa cggtaaccct
     9361 gacctttatt gaccaaagga ggaaggatga gaactgaaaa agacattgaa aattatttga
     9421 aaaagaaaac aaaaggcctg tgtttaaaat ttaccagtcc agggacggta ggagttccgg
     9481 acagaattgt tgtcatgaaa actggtgtat tttttgtaga ggtcaaagcg cctggtaaaa
     9541 agccaagacc gagtcaaatc ctgatgcaca aaaaaataaa ggaggtaggt caaccagttt
     9601 gggttgttga ctcctacgaa tcagtggaca tagccttaaa agaaatggag aactgggtgt
     9661 gagactgcat gagtatcaag aatacgctaa gacatggata gtagagcacc cttattgtgg
     9721 ccttttactt gacatgggcc ttggtaaaac gctgacaaca ctatcggcaa tagatgagat
     9781 tcaaaatatt ttttccgagg atcataagat tttaatcgta gctcctaaaa aagtggcgga
     9841 agaaacgtgg ccaacggaga ttgggaaatg gcattttgat ttcacctatt ctaaagtttt
     9901 ggggagtgag ggaaaacgaa ttgaagcctt agaaacagaa gccgatatct atttgattaa
     9961 tcgtgagaat gttacttggc ttgttgaata ctacaagact aaatggccgt ttacctttgt
    10021 tgttattgat gagctgtcaa gctttaagtc tagtaagtca aaacggttta gggctttgcg
    10081 aaaagttaga ccgaaagtcc aacgccttgt agggctaaca ggaaccccag cgcctaacag
    10141 tttgattgat ttgtggccgc agatttatct gatggacaga ggcgacaggc ttgagacgag
    10201 ccagactcga tttaaagaca agtattttgt tcctgataag cgtaatggtc caatcattta
    10261 cagttgggca cttagggatg gtgcagaagc agaaatctat aacaagattg aggacatctg
    10321 tgtcagcatg aaagctaaag actatctcaa gttaccgccg cgaaccgaca acgttgtatc
    10381 agttaagtta tctaatatga aagcctacaa acagtttgaa gctgatttgg tgttggagtt
    10441 taaaaataaa gaaatatctg cggctaattc tgcggttttg gccaataaat tacttcaaat
    10501 ggccaatggt gctatctatg atgatgataa agcaacagtt gctatacacg acgacaaact
    10561 tgacgcgctt gagaacatag tagaagaaag ccaaggccag cctatcttag ttttttacca
    10621 gtatcaacat gatcttgaga gacttaagaa acgatttcct caggctgaag agctgacgtc
    10681 ggttgacaag tggaattccg aaaaaatacc aattcttctg tgccaccctc aatcggcggg
    10741 gcatgggctt aatctacaaa aaggcgggca tattattgtt tggtttgggc taacatggag
    10801 tcttgaatat tatcagcaag ctaatgccag attagataga caggggcaga cagaacccgt
    10861 tattgtgcac cacattgttg cagaaaatac agttgatgaa aaagtactta ggattttaca
    10921 aggcaaagaa aaaaatcaga acgccttact tgaagcagtt aaggcgcagt taggagtcta
    10981 gatgaaaaaa gaatatgtcg ttagaatcta cacgggtaga gaaaagaatc ttgaggcaaa
    11041 acctcagttt gaagagaaaa cctttacgcg aaaagcggac atgttgaagt tttggaattc
    11101 ttgcgaagca acagttaaag aaaaatatac tagggagcgg gagaatgatt gaagaacaat
    11161 aaatacttga ctgcttagtc gacaaaatgt ttcagcagga cacaaaagca acttaaacgt
    11221 gctagcgagc aaaatcagag acagacaaag cggattgctg atttgactgg aaatgggggg
    11281 taggtatgaa tataatccat agaatggaaa acgggataaa cgctgcaggt gatttaataa
    11341 cttatgcagt accatgttgt ccagagtgta aaagtgaaac atatagtcag aaacactgtc
    11401 ctttttgcgg tgaaaatctt atggaggtag caaatgaaaa ttgaattttt tttaaagcaa
    11461 ggtcctacta agagcgtttc taattatggt gaagttggta tcgatgaagt aattggtgca
    11521 ataaaaaata acgatatcgt tgcgctaaat ggaaaaaaca acgcagttat tatacccaaa
    11581 caaaatattt ctcacgtaat tattcataaa gaggtgacga atgaataaat tatctaacgc
    11641 acagctaaag gcttttgatg aatggctgtt tgattatcgt tttattgata acaagattgc
    11701 acttcgaaaa cttgagctac aaactgatat gaatacagaa gtagataaaa atatcggtgg
    11761 aggaagagca aattttgttt caaaagttac tgaggatgtg gttgcaaggt gggattcaga
    11821 tagggagcta aagggtctat ccaatttcaa agaagcagtt attgcaacgc taaatctttt
    11881 ggatgaagag ctaactgata tttttaatct tagatggggt gttggttcaa gcaacacttg
    11941 ggaagaaatt gcctatacaa gacatctgtc gcgtaagtct atttaccgca aaagagagcg
    12001 gattctagaa atattctcca aaaaaatagg aatgtgacac aaaaatgtat ggaattgtca
    12061 tctttttgat gataaattga tagtatgagt ttaaaggtaa ggcaaatagt gtcttgcctt
    12121 tttgttatgc ttggaggtga tggaaaattg agtaaattaa ccctaaaaca gaagcgtttt
    12181 gcagatgagt acatcatctc agctaacgcg acagcagcgg ctattaaagc agggtatagt
    12241 aaaaagacag caaggtcaat aggtcaagaa aacttgacca aacctgacat taaagcttat
    12301 atagacgagc ggcttgaaaa acttgaatct gaaaagattg ctacgcaaga agaagtccta
    12361 caatatctca cttcaattat gcgaggagac caacaagaaa agacgctcat tagcgttgga
    12421 gagtttggcc agaagatagt tgacatcgat gtaggtgcaa aagatagaat caaggcagct
    12481 gagctacttg gtaagcgata tagattattc accgataaag ttgaaatgga tgtgtcatca
    12541 gatataacta ttagcgtagg tgagtgggat gacgattaaa cagcgcccta aaattaatat
    12601 cgtaatcaag catcctagca aagtttttaa caaacatatc tacgacaaac tttacaacta
    12661 tagcaacttc acggaagttc attatggtgg ggcgtctagc ggaaagtctc atggtgtttt
    12721 tcaaaagata attttaaaag cacttaaccc caaattcaaa catcccagaa agatattagt
    12781 tctcagaaaa gttggtgcaa cagttaggga ctctgtcttc gctgatatta tgtctaattt
    12841 gtcgtatttt ggcgtcttgg ataaatgcaa gataaatatg tcagctttta gaataacatt
    12901 gccgaatggc tcggaattta ttttcaaagg catggataac cctgagaaga ttaagtcaat
    12961 taaggggata tctgatgtcg tcatggaaga agctagtgag tttactcttg acgattacac
    13021 acagcttacc ttacgtctta gggacaagaa acatttagag aagcaagtct atctcatgtt
    13081 caatcctgtg agtaaagcaa actgggttta taaagctttc tttatcaagt cacctaagaa
    13141 tacagttgtc tatcaaacaa cttataaaga caaccgcttc ttagatgatg ttactagaga
    13201 aaatatcgag gaactagccg ataggaatga agcttattac aaaatatatg ctcttggcca
    13261 gtttgctaca cttgataaac taatttttcc caagtatgac aagcaaatat taaacaaaga
    13321 caagttatca cacttgcctt ctttttttgg tttggactat gggtttatca atgacccttc
    13381 ggcatttttg catgttaaaa tcgatgacgc aaacaagaag ttatacatct tagaggaata
    13441 tgtcagaaaa aatttgacca atgataaaat agcaaatgct ataaaggacc ttggctatgc
    13501 caaagaagaa atcagaggag attcggctga aaagaaatct aaccaagagc tgaggaattt
    13561 aggtattcct agaatgattg atgttaccaa aggtcctgga accgttatgc aaggaattca
    13621 gtacctgctt cagtatgatt ggattgttga tgaaaggtgt gtcaagacta ttgaagaact
    13681 cgaaaattac acttggaaga aagacaagaa gactaatgag tatatcaatg aaccagttga
    13741 cagttacaac cactgcattg acgccataag atatgccgta caagacagaa tatatcagtc
    13801 ggcagataga agtaagcgca tgaagaacgc gaagtactat ttttaggaat gaggtgagac
    13861 tttggaagaa aaacaatttt tagtaggaac tcgttttaat gagaatgcta ataagcaatt
    13921 tattatgctg caagaagact ttgaagcaat tgactatgaa tcaaaacttt ggatagatca
    13981 acttaagaat tacatttcaa gatttaaagc agaacaatta gagcgtttga aagagttaaa
    14041 gcgatactat cttggggata ataacatcaa gtatagacct gctaaaacag ataaatacgc
    14101 agcggataat cgtattgcaa gtgattttgc taagtatatt acggtatttg agcaaggtta
    14161 tatgcttggc gttcccgttg agtacaaaaa tgaaaataaa gacctccagg cagctattga
    14221 ccttatgtcg gtcagaaaca atgaggatta ccacaacgtc aaaataaaaa cagatttatc
    14281 aatttatgga agagcctacg agctgttaac tgttgaggaa atagatgaca aaaaaaccga
    14341 agtaaaactc taccagctcc cagccgagca aacttttgtt atctatgatg atacatacca
    14401 gcgaaattcg ttgatggccg ttcattttta cgacatagat tatggctcag gtaaacgcaa
    14461 acagattatc aaagtatata cttccgacac aatttatacc tacgaagact ataatttcga
    14521 aacaaaaggt atgcgcttaa aagagcatga agaacatttt tttaaaggtg taccagtcaa
    14581 tgaatatgct aataatgagg agcgtacagg ggcttatgag tctgtacttg ataatattga
    14641 tgcctatgat ttatctcagt cagagcttgc taattttcaa caagattcag tcaatgcgtt
    14701 gcttgtaata gctggtaata cttacacagg ggctgatgag aatgactact tagatgacgg
    14761 acgattaaat cctaatggtc gtttagcgat ctcaattggg tttaaaaaag cccaagtgtt
    14821 aattttagac gataatccta atccaaatgg cgttaaacca caagcgtact ttctcaaaaa
    14881 agagtatgat accgccggta gcgaagccta caaaaacaga ctagttgcag acattttgag
    14941 atttactttt acgccagata cacaagatat gaaattttca ggagttcaat ctggtgaatc
    15001 aatgaagtat aagcttatgg cttctgacaa ctatcgcgag aagcaagaga ggttgtttaa
    15061 aaaaggctta atgcgacgct tacgcttagc agctaatatc tgggccatca aagggaatga
    15121 agcaactaca tatagccttg taaatgatac gagtatagtt ttcacaccta atcttcctca
    15181 aaacgataat gaaattgtta cagctgcaca aaatctctat ggcattgtta gcgatcagac
    15241 tatctttgaa atcttaaata ctgtcacagg agtagacgct gaagcagagc ttgaacgttt
    15301 gaaagaagaa gctgataaaa agcaatcttt accggagcct agattggtag gtgatgctag
    15361 tggtcaagaa gaaccaacag cagaaaagcc ttaattactg gcaaaaacgt caagaagata
    15421 ttctaagtta tttagacaga actgatttag acgtcttttc agaactgcaa aagttatata
    15481 atgaacaggc ttttgagtta caaaaggaat tgtttgattt ttatactaag tattctgaag
    15541 agaacaaaat gacttatcag gatgtcgtta aaaagctacg tcatgaagat ttatcagatt
    15601 acgtagctaa tgctaacaag tatcgtaaac aagctgagaa agaccctgag ttgctaaaac
    15661 gacttaatga gcagtatgta tcagcaagag ctacaaggat ggatgcatta aatcttgaac
    15721 ttgtttatcg cgcagggata ctaaaaggtg tacttgattc agtatttgaa aatcatttaa
    15781 aaaaagctgc ctcatatgct tataaaaaag caatgggtgg acggtcaggg acaatcaatg
    15841 gtccagtttt agaagaactg gttagaaccc cgtttgatgg ttataactac tcagaacagt
    15901 tgtggggcaa tactgacaat cttgtcaaga atctccaaaa gaaattaaaa caaggttttg
    15961 ttcgtggcga gcatcctaga gcgatagcca gagatttagc taagcaattt aatgttgcta
    16021 atcacagggc tgaaacacta atcagaactg atggaactat ggtgattaat aatgctaccg
    16081 ctaggcgcta cttgaatgca ggacttaagt attatcgtga tttagttagg cttgatgaca
    16141 ggacaactga gatatgtcgt aaaatcgcta aagaaaacaa aagaaagcta ttatctgagc
    16201 taaagcctgg aattaatgca gcaccctatc atttcaactg cagaacaacc attattcctg
    16261 atgaagatga gctaagcatt gaagtagatc caattgatga taaaagcact aagtatttta
    16321 aggatgtcac ctcagattgg atagatggaa atgagcataa accacaactg tcactactaa
    16381 atgagtatgt aaaaaatggc actccttaca aagtagatgg ccataatgtt gttcttgatc
    16441 actcgaatta tgaatataga gttgctaatt ggttatctaa aaagacagga ttgcaagttg
    16501 acatggtgcc aagagttaat tcccctgaac atatcaatac tcctgattat ttagttgatg
    16561 gagttccttt tgatctaaaa gaaatcacag gttcgggtaa aaacgtcatt gatggcaatc
    16621 tacgaaaagc aaaaaagcag gcaactaata tcatttttga tataacaaaa acccctttat
    16681 catttgaaga gataatgggg cagttagaac acatttatat gttagatcgt aggggtcttg
    16741 atatatcaat tatcaaaaat aaagatgagg tattagctgt attagaaaaa gaaggacgat
    16801 gacccaccgc ctccacagta aactgcttca tgggcgttag accatcgtcc ttctttatct
    16861 caattataca tcaacctatt aaaactatca aggaggaaaa tatgtttatt tggcaaatgg
    16921 tattagtggc actaggtgta ttggtgctca tattgattgt tggcattgca gcaatagcgg
    16981 ttaagtcaat tattgcagaa ttgaaaaagg taggataaac atgaataagc gcattaagaa
    17041 aaaacgtaaa ttggaaacag cggttgtgat gcttgttgca gagaatgcca tgcaagcaga
    17101 agctcttaga aatcaaaaca aacaaattac agagctgaga gcgattgtac aacaaaacgc
    17161 ccaagctacc aatgaagagt ttgcgtctgt taaagctgct actttagata accaatcagc
    17221 tattgcagat attgcaattg acgttaacta tattaagaaa aattacaagc gaaaatgggg
    17281 aaaataaatt ttaaacgaat tcgacccctt tagaaatcaa agtcgtagcg atgcggcctt
    17341 ttattatgtc caaactttgt tgatgacact aaaagctaca ctgtttcgtc gcaggacgta
    17401 aaactagatt atcggttggt ggcgtaacca ctaggagaaa ttatggcaga agaaaatgta
    17461 acaacagaaa ctactgaaca agtcgacact cagcaagaaa ctgttgaaca tcctaagcag
    17521 gaacatgagc gaacttttac tcgcgcagat atctcaaaaa tgatgactgc tgaacgtgca
    17581 aagtgggaag cagagcaatc agaagcaatc gaaaaagctc gtactgaggg tgaaagacta
    17641 gctaaattat caaaagatga acgtgctaaa gaagaagaac aaaaacgtct cagtgctatt
    17701 gcagaacgtg aaaaagcagt tgcagaacgt gaaatgcgca tcgaaacaca atcattactt
    17761 gctgaaaaag gattgccact agattttatt gatgttgttt tagctactac tgcagatgaa
    17821 gtgaaaacaa acattgataa tttacagtct atctttgatc aagcagtgga aaatcgtgtt
    17881 aacgaaagat taacgcaaaa gtcacctcga actggtaatg gctctgtcgg tatgactaaa
    17941 gctgaaatta tggctattga gaatgatgat gaacgtctca aagctattgc agctaatcgt
    18001 aatttattta caaggtaaga aaggacatat taatttatgg cagaaaataa tttaaatgtg
    18061 atgtcagact taggagaaat caaatctatt gattttgtta ataagttttc aacgaatatc
    18121 aatgaactac tcaagctttt gggagtaact cgcaaacagg aattgactaa tgatctaaaa
    18181 atccaaactt ataagtggtc tactacttta gataagacaa atcccgccga gggtgaaaca
    18241 attcctttat ctaaagttac tcgtgcaaaa gctaaggaat atactgttga gtggttcaaa
    18301 aaacgtcgtg caactaccgc tgaagcaatt gcacgtcatg gtgtagctcg tgcgattaac
    18361 gaagcagata cacgcattat gcgagaaatt caaaatggtt ttaaaactaa cttctttgaa
    18421 tttttaaaga agtcaactac aaaagtgact ggaacaactc ttcaaagtgc attatcacaa
    18481 tcatgggcaa aaattgctac ttttaatgaa ttcgatggtt cacctatcat ctctttcgtt
    18541 tctccacttg atgttgctaa gtatctagga gatacaaatg taggtgcaga tgcttctaac
    18601 gtgtttggaa tgacattgct taaaaacttc ctaggtatgc aaaacgttat cgttttacca
    18661 tcagtgccag agggaaaagt atacacaaca gctgttgaaa accttgtgtt cgcttattta
    18721 aacgtcaata gtggcgatct tggagggatg tttgctgatt ttactgatga aactggattg
    18781 attggtgcgg ctagaactcg tcaactttct aatttgacct atgaatcagt attttttggt
    18841 gcaaatgtgc tatttgcaga aattccagaa ggcgttgtag aagcaacaat tgaggctcca
    18901 aaagtagttc caggaggcta atagatggat aaggagttat tactcacaga ggttaagcta
    18961 ttcaaaggga tcagcgccaa agacactagc caagatgatt tgataaaatt atccattaat
    19021 gaaagtattg accgtatcct tgcgaaactc aatgattact cagacacaga aatgactgaa
    19081 attcctaagc gtcttacttt tattgtcaga gatgtggcta ttaaaaggta taacagactt
    19141 aattcagaag gaaccgttga agatagcgaa gaaggaaaga cttttaagtg ggacagttac
    19201 cttaaagagt atgaatcaac actcagaagc gctgctattg ggaaggtata ttcaggcaaa
    19261 ggggtagcaa gatttattta ggaggtcata aatgatttac aaagatagag ttatcctagt
    19321 gtatgtcgat gagcaggacg attttctaga taaaagaact gttgaaaaac ctagcggaaa
    19381 aatcccctgc atggaaaata ctttcacaaa atctgaacag atggggttat ttggtaaata
    19441 tgatttgaac gcttttaagt tgcacttgca aggccgatac gatggcttta gcaagattat
    19501 ctacaagggg aaaccgaggt tgataaaggg actagcgcac cataaaaata gcacggttat
    19561 ttatgtatga gtcttattta tcggatgaga ggcctagata ggtttttacg cagcgttgag
    19621 cgcaagcaga agtcagtacg aatcgctgta gataaagagc ttagtaaatc agctgctagg
    19681 attgagagac aggctaaaat actagccccg gttgacactg gatggctgag agctcaaatc
    19741 tacagtgagc aacaacgact cttacactac agagtggttt ctcctgcttt atattctatt
    19801 tatcttgagt taggtactcg taaaatggaa gcccaaccat ttttagaccc tgccctgaga
    19861 aaagaatggc cagtgttaat ggctaatctc aaaaaaatgt ttaagaggtg atgcatggat
    19921 tactcaccag aaacactata tttaaaaaag gtaaaaaata gattgggagt tttagacata
    19981 ccaatctatt ttaaattgcc taaatcagac gttttagagc cttttattgt tgtaggtaca
    20041 aatatatcag acttgtcaaa aacagctcaa actggagcag ttattgatga ttttagcctg
    20101 aatatcgatg ctttcttacc tggcgatagt cgtttggatg cagaagagat aaaatctcgc
    20161 atgcttagac tgcttgggcg gaataaccaa ataaaggctc agattttggt agataattca
    20221 ataggacgag aagtctatag agttgctatc aacattacag aaatactaat ttaaaggaga
    20281 attaaatgga aactacagtt gaaattacta cagctaagcc tattaaaggt aaaaatatat
    20341 tttattttat tcaatctgtt gatgctgaat taggtagtaa agcaatactg ccagcgtatc
    20401 gtacagatgg aacaacgaag ctcggtggag attttatcga tgaacaaact caacaaggac
    20461 gtgtgcttga aaaagcaaca gatgagcact caattgattt gacatcatac tacacgccga
    20521 aagacaaagc aattgaaatt gtagaaatag ctaaaacaac tggtaaatct gttaaagttt
    20581 ggcgtgttat cgcagacgaa tctttgaaag aaaaaggttc aggaacttta gagggtaaag
    20641 aagtttatcc cgcgaagttt ggatatggaa aaattgacga gattgaatat tcagaaggtg
    20701 ttgaaaaact tgtagaagca tcatatacat tgaatattat cgatagttta aaagatggca
    20761 aatttccact gacggcagaa gatatcgctt tgttaacaag tgtttatgca tatcaaaacc
    20821 caggcgaaac tactggcgat tatgacaata tccagaaaaa agaagtttca ggaagtttac
    20881 tgggagcagt tccagggata tcatcagaag gacactaatt tataaggtag catttcgcta
    20941 ccttttattt ttaaggagaa atacataaat ggaattaaca gtagcaaaac gcagtattga
    21001 aattaaattt gattttaaaa caatgtttaa gataaataac agacttggaa ctgtgaataa
    21061 agagacaggg gaacgtaacg ctgacggcgt cggtacgcta ttttataaaa tcttagagcg
    21121 tgatgattca gctgtagtag atttagtcaa gctgtctgta ggcagcggta aaaaagcatt
    21181 gtcagaagat gaagtacttg attctatcgc agagctagtc gaagaagaag gatcgacaga
    21241 agccttattt aaagaaattg agaatgacat ggttgaatct ggttttttca aagagaagat
    21301 ttcgaaatat atcgaaagca tggagaaatc agtgaaatat ttagaagctc gagaagatgc
    21361 ggacgaagct caaatccaaa tcgtaaaaga catgattggg aaaatgaaag acgcaatctc
    21421 ttaattaact gtgcccaact tggcctgacg gacattgata ttatttacaa gtgtacgaga
    21481 acagaattag atgcaattat ggaaggattg cagtacaagc aaattgcaga acgtgaaaac
    21541 ctatctgaac ttgctttaaa tctcagatac acgttaaatg ctaaaaacgt caatgctaac
    21601 aagctcaaat tagataaaca acgtaacaaa attaaacgaa cttatcaaaa taaacaagat
    21661 ctaactaacg tttcaaatga tttcgcagaa cgtgtggcga agctcaattt acattttcaa
    21721 aatttataac aagaaaggag ggattttatg gcaggtacat ttgatgggtc tatattcgct
    21781 gatattggtg caaatactaa agattatgag gcagctatgg ctagaattgt tagtactaca
    21841 caaaatgctt ttagaaaagc gcaggatact gcagttaaca acagcaacaa aatgatacag
    21901 atgattggtc aaattatggc tcaattagca aataatggtc aatcattagg gcaaagactc
    21961 ggtaatgcat atagtacagg tctcaagtta tctcttggtc aaattcaaag gatagcagca
    22021 tctatcggtg aaaaaattcc cgaacccata aagaatgggt tcaataaagc tttttcaatt
    22081 attcaaaata gcgtacagaa gttatcgagc gccatccctc aacccataaa atccgcattc
    22141 acaagtgcaa caagcgcagt agttagcttt tcgtctaaga tcacaagtgc cgtctcagct
    22201 gctttcaact ctgttagttc taaagcgagt actgttgcag ataaaatcag tagtagtttt
    22261 gggggtagaa tcacttcagc ggttactaat ttagcgacta agttaagcac aggtcttagc
    22321 aatggtttta gcaggatgtc cagttctgca actagtgccc taaatggtat tagtcagaga
    22381 ttcgcaagcg catcatctgc tggtgaaaaa ctaagaagca ctgtgggaag tatcatttct
    22441 gcttttagtt tgatggccat tgctcggaaa ggaatccaag caatcacagg agcaatggac
    22501 ggtgcaataa gtcgtgtaga taccatgaac cgctttccga aaacgatggc gctatttggg
    22561 tattctgcac aacagtctaa agcatcaata gataaattat ctaaaggaat tgaaggactt
    22621 cctacaccac tagacagtgc tgttaaaagt gcccaacaat tagctataac tacaggtagc
    22681 ttagataaag gaacagagct agcattagca tttaataatg caatgattgg ttatggagcg
    22741 actactgaag gggctgagca agcacttaga cagtttaacc agtcgttggg gtctggtaag
    22801 atatatgccg aagagtttaa ttctgtgtct gaagctgccc ctggcttaat gtctaaaatg
    22861 gcagagtcat tcggatttgg taaaaatggt gttcaggact taaaagcagc tctttcagac
    22921 ggcagtatta ctgcccaaga attcgctgat aaaatgattg aactaaacaa ggcgcagggt
    22981 ggttttgctg agatggccca atcatcggct ggtggaatcc gaactgcatg gaagaacgta
    23041 cataccgccg tcgtaaaagg cgtagcaggt atgatttcag cctttgatga agctgccaaa
    23101 gctaatggta tgaagaccat tgcagaatcc attaatagta ttaagccagc tataaattct
    23161 gcatttgcaa ctgtgaactc aataatgcca aaccttgttg cagcattcgc tagaatctcc
    23221 aagatggtga atattgactt tagtccacta actagtggaa ttaaatcagc ttttggatta
    23281 ttaaatactg ctttaggtga atttgcaaaa actggtgaag tatcgtcagc aactattgat
    23341 agaatcaaat caaagatagc atctataggt cctactgcaa tagcagcttg ggccttatta
    23401 aatcctgcaa gtgctattgc aactattatg cctttaatat caattattgg taagattggt
    23461 gttgctcttg ggactgttgg ttcagcgttg ggatcattcg gagaaatggt agccagtgga
    23521 ttgtcaactg ctagtgggct agttggagct tttggtagag ctatgtcagg acttcctgga
    23581 atatttggtg tagctgcaag tagaggtctt gctgtacttg atggaatgac aactgcgatt
    23641 agtagtgctg caatactggc aatgtctgct ataggtcctg cagcaatttt aggattggtc
    23701 gtagtaggtc ttggtttagt aaacaatcaa tttggtactc aaattgataa attgataaat
    23761 acagcaatta ctaaaggacc tggtattatt caaggccttg taaaaggcat tacttctaaa
    23821 ataccagctt tgatagctag tggtactcaa ttgattgcta agtttgctaa tgctctaaca
    23881 gtattattgc cagttattat tcaggcaggt gttcaattaa ttaccagtct ggtgcaaggt
    23941 attggtcaaa acgcaggtag tttaattagt tcggctctta aagttattgg aacttttgta
    24001 agttcaattg ctagtgctct ccctcaaata ctagctgtag gtgttcaatt aatactgaac
    24061 ttagctcgag gtattgtaca gaatatacct ttaataatcc agtatgctca acaaattatt
    24121 tctaattttg gttcaagctt acaagctaat atgccaagca taattagcaa cggtatagca
    24181 attagcacca atttgaatgc tggtattatc caaatgttgc cggcagttat acaaattgca
    24241 acccaagtca tcacaggttt tatttccggt atagctcagt atttgccaca ggtaatccaa
    24301 ggcggcatcc agattataat catgttagtg caaggtatta ttcaaaattt accgcaggta
    24361 atccaagcag cgatgcagat tattcagtca ttgatatctg gtttatcaca agcattacca
    24421 caaattatat ctgcagggat acagttagtt gtgcagttgg ctgttgcaat tattaaaggg
    24481 ttgccgcaga ttatttcagc tggtattcaa ttgattgcgg gtcttggtaa agctatgctt
    24541 gaggctattc cgaatgctct ttcaggggta tgggaaggca ttaaaggtgg atttagttct
    24601 atgtgggacc aaatcacagg aaagagctca acaagtgccg ctaaggttac atcagacatg
    24661 tcaacaatga ctatgaatgt tggtatgcag acgaccacaa tggctaatca aactaacata
    24721 gacacaacta ctatgctaaa tagcattagt caaaatactg gtcttgctaa tttaaatgcg
    24781 acaacaaatg ctactcagat ggcaacaaat gtaaatgcac aaacagcatc tatggcaaat
    24841 caaggtttgc tgaatgcggc aggatttaat gaagcgttga gttcgaacat gactttagca
    24901 aatacaaatg ctactaatca agctaatcaa atgactgcta atgttaataa cgctactagc
    24961 caaatgagtc tgggttctct accgaatttc ttggaattat cgcaacaggc aacaagtaat
    25021 atgcaaactg cacaagcaaa tgctaccact tccgcgaatg gaatgagttc tggagttact
    25081 ggtgctacag ctggtatggc tagtggtgta gctaataaca tgttaggtat aactagcact
    25141 gtatctacag ggatgaatca agctgcaaat gctgcaaaat caggcggtgc aaacatatct
    25201 aatagtgtga atagcagttt tacacaagcc aaatctacag tttcaagttc aatgtcagga
    25261 atgaacagca ctgttactag tggaatgaat agtattaata atactgtaaa atcaggtgga
    25321 aataatatta aatccacctt tacaagttcc tttaattctg ctaagtctac agtttcaaat
    25381 gctatgtcta gtattcgttc gtcaatttca agtggaatga acagcgctgt gagtgtaaca
    25441 agaagtgcaa tgaattcggt tgtatctgca atgaatgcag cagcaagtgg tgctagatct
    25501 gcaggttata atactgggat tggatttgca aatggcctag caagcactgc aggaactatt
    25561 atggctattg ctaatagtat tgctgctaat gtcgctgcaa caatcagaag cgcaatggat
    25621 attcattcgc cttcgcgagt aacgcgagaa cttggtgctt atactggtga aggttttgca
    25681 cttggtatgc aggattggat ggatgatatc aatgcaatag gtaaagagta cgctaatgct
    25741 gtgactgatc agcgatggga aacagattca caattagcga catctgcaaa catatcaagt
    25801 gcaggtgtat catcatcact tgataatcta agtgacgagg tacgcaacca acaattgact
    25861 agtccaacgt tcgaggttca taatgaactt gttggtgatg aaatttatac aactattaaa
    25921 tctaaagaag caagagaaag taataaagat aattacttca actttgcgac ataagagagg
    25981 agaaaaatgg atttattagt acaaaagagt gatcaatcct ttcgattttc agagattggt
    26041 ctacgaatac ttgatgtcga tgaccgttca tcatctcttg aggttgatag tcgaacagtg
    26101 aaaggtcgaa gcggtaaaat ctttgctagt gctaagtatg gcacaaaaaa agttaaggtt
    26161 aatgggcgct tagttgtagc ttctattaaa gattttatga caaaaaaaga tgatgtgaat
    26221 ggcttactag tggatagtga gcctttttac attactaaaa tgtatcctca gaaagaaaat
    26281 ttatatgatt tcgaattgcc tgggatgaaa gcaggtgatt tagacttatt aaatcaatct
    26341 tatacagcgt ggcactatcg ttggaaagtt tatgtctcaa gtgaacctga gtacactttt
    26401 gttggtaagt ctagtgaagg tctcaaatac aactttacaa ttgcatttga gactgcagaa
    26461 cttccatttg gtgaaactat tgcacaaaat atagtcatca ctaacggaac gtttaattat
    26521 tctggtactg caccttttag ccagttagaa tacccatggt ctgtagagtt aacttcaact
    26581 ggtggacaaa gtagttttta tcttgaaatc gatgggaggc gctgggctta tcaatatagt
    26641 gcaccactta atcaaggcga cgttattgta atcactggta tatccacaac attgaataag
    26701 ataaatgtga ctgctagaac caatcatgaa tattttgttt taaagcaaaa agtcaacaag
    26761 actattctct acagcacaaa ttttcaaggc acaatcaagt ttttaaactt taaagaactt
    26821 tacaagtaag gaagtgatag attgacaact tttttagatc ataaagataa tgagtttgaa
    26881 gctcaagctg ttgttaaaac aactaatgca gttaacggag aaaggtcttt atcaggaaca
    26941 atttatacta acgaagaggt actaaacaag gtcgataaag gttggaaact tgagtttgat
    27001 gatgagtatt acaagattat ttatgctaaa cctactgata ctggtaacaa aattcaagtt
    27061 gaatttgatg cagttcatca atttttttat gattttgata aatcatcact acataagcaa
    27121 ttaaatgatg gttcacatac tttccaagca tatttgaact ttatttttga tggcagcggg
    27181 tatacttaca gtttagaagt agttgtcaaa tctttcgaaa agcaatcgtt tggatataaa
    27241 tcgcgacttg atttatttaa tgacatcatt aagagtgcag gagttgaatt ttttgttcgt
    27301 ggcaaggttg ttcgaattct acaaaaaaca ggaacagatt tatcaacaat cgtacgtaaa
    27361 aatttcaata tgaatgaaat cataattgaa aagaaaatca atgattttat tacatatcag
    27421 aagggttttg gagcgtgggc tgaccaagag gatcattcca aaggaaggct cgaagtagaa
    27481 tatgaaagtc cacttgcaaa ggaatatgga cgaattgagg gtgagcctgt tgttgatgag
    27541 cggtacacta acgctgacaa tttaaaatca gtactaaaac aaaatgtaga ttcgtcttac
    27601 aatatcgctg taaaaattga cattgaagat cttacaaaag caggttatca gtacactcaa
    27661 cctattgccg gtgattatat tatggctatc aatgagacac taggatttaa ggaaaaaatc
    27721 cgaatagtat cgtttgaaag tgagtatgat gtaacaggtc agctgattaa ccataaagtc
    27781 acttgtaacg atattggtac aatcaaaaaa caaactgtag ctgttagcca attatcaagt
    27841 aagattaata ataaccaagc aatactagat aatgcagtat ttaaagcaaa tcaagcacta
    27901 gcatcagctg atggtaaaaa cactaactac tatggtacgg agatgccagt agataatcca
    27961 aaaggcacgc taagaaaggg tgatttgctt tttttaactg ttggcgatac taccagaatg
    28021 tatttctgga atggtgctga atggattatc aattctttta gcgacgatat tgattttgta
    28081 aagaaagaaa ttactaataa aatcatagag gttaatgcag caatgaaact tgctgatgaa
    28141 cagttaaccg aaaaatataa tgcaattgtt gctaaaaatg ttagccaaga cgaattgatc
    28201 aatcaagcta aaactttatc tgactctgca aaaaaagagg ctacagaagc attaacaaat
    28261 cttattagtg aatctaaaaa actgactgac cagatgtctg cgttatctaa aacagaagtt
    28321 gagcattatt caacgactaa tcagcaactg actcaaattg atggtacagt caagggttta
    28381 caaactagct atgaagcatt gttaaaaaaa gatggagata tcactcaggc acttgctaat
    28441 tataagcaaa caattgacca aaacagtgca agtattatga ccaacaaaaa gactgccgat
    28501 aatgcactta gcagtctaca gacacaagtc atacaaacgt cgaacgaaat tacaacgaga
    28561 ttatctcaaa caaatattga agcgttgatt acaagcaagt ctgccgttat cgctgataat
    28621 aaagtcaagg aaactgcaga tagttttagt agagaattaa ctcgtgttaa taatactatt
    28681 gataatttaa agataggtac agttaatcta ctatctggta ctaaaggttg gactaaaaat
    28741 tggtttaatc gtaacaactg gcaagctgat gatgacagct tgtctattgg taatttcaac
    28801 ttaacagtgc taaagcgtaa gatgatgtgg agtggtatat cacaatcata tgctgtaaaa
    28861 agcggagaga gttatgtttt tagtgcatat gctaaatcaa gcatagataa tgatgttgtt
    28921 aatttatatt tgttaccaaa tgatcaatcg caacaggcta agataagtat tagtttcaag
    28981 tcgatcactt taaacacgga ttggcaacgt atcgttatta aatttaacgt aaattctgat
    29041 ggctatattt taccacgctt cgaacgcttt aatgaaaatg gttggttgta tattgcaggt
    29101 taccagcttg aacatggaaa tgttacaagt gactattcag agtccccagc ggatcaagaa
    29161 gataaaatga ctgttgaatt taataaaatc aacgatacag ttaatagtca tagtcaactg
    29221 attggtaaac aaaacgagag tctgacagct acaatacaaa aagttgataa tatacaatca
    29281 acagtatcta atgtggatgg acgcttgtca cgagttaccc aaacggctga cggtatagtt
    29341 gcaacagtta gtcagcttga taacaacatt ttacctggca caaaaaaatt cacaggatgg
    29401 caaacagaag gtgcagtact agaggaacta acacaatcgc cttacccatt tgtttttaaa
    29461 aaatgggtaa gcggtaataa ggtgtcacct ttaatcgaat atgatgtcaa aaaagatcaa
    29521 gagtatacgt ttactgcata catttctaga gaacaagcag gaaatctata tttctacttg
    29581 tatgatcttt ggaatcatca tattacaagc acaactcctc gtgaaactat tattaaagac
    29641 gtcactcaaa ccattagacg atttaaaata acatttattc caactcgaga cggtaaaatt
    29701 agaccacgct ttgccatgct tgctagtgat ccaggtagct ttagtaccgg tggttttatg
    29761 ttatctaaag gtacagttga gttggaatgg tctgaatcta agcaagatat taaagatagt
    29821 gtgactgcta ttaatacaac agttacacaa acggctaacg gttgggctat taaaaattta
    29881 acttctagcg gtactgtgtt gagtcagctt aatttgacgg acggtaatgt aaaactggaa
    29941 ggtaagttaa tacacttgtc aggacagtct attatcgaca atggcattat tactaatgcg
    30001 atgattaaag atttaactgc agataagatt acagcaggta caattgatgc taatcgtata
    30061 aatgtcatta atttagatgc tagcaacatc actagtggca ggatgtcagc agatctgata
    30121 cgtagcggcg ttttaatatc gcaaaacggt gcaatgttag cagatcttaa tacaggacaa
    30181 attgaatttt acacagatag tccagctatc aaacgtatta ctgcaggtta tcctaaccaa
    30241 ttcgttaaat ttgcaacagg taatgttgaa ggaaagggcg cggctggcgt aacggttata
    30301 ggttctaatc gatggggaag tgaatcgtca aacgacggtg gtttcgtagg tattcgcgct
    30361 tggaatggta aagacatcga tactcttgat ttagttggcg atagtattca cttagcaagc
    30421 tctgcgtaca ctaacgctga tggttgggaa atcattactt tgccaaatca gctatcaata
    30481 gatgcacgta acataaatca tcgtgtaacg tctaagataa aagtaggaga tatttggcta
    30541 tggaaaaata catcaactta tacaagcatg aaagatacaa ttaatatgat tattgataac
    30601 ttgcagctac tacacaataa taaaaccact gaaaaaggct acagttatac tattccagga
    30661 aagatttagg agaaaaaatg gaagaaaaaa taaaatcaaa tttagcttta caaatagcgc
    30721 aattatcttt agataaagca actctacaag ctcaaaatga gcaattacaa ttacaaattg
    30781 ctgagttaaa tcagcaatta gatgagataa ctaagccagg agcaaagtga ggtgagtaac
    30841 atgaaacaat ggaacattgt tggaaaacca tttccaatct atcaagatgg agtggttcta
    30901 aaaacggatg tacgcttaca aggcgacgat ggtacatatt taccacaaga gctaattggt
    30961 aatcatgttg ataaggataa tcaatcactg ataaaacttg tacttgaaac ttttgcaaaa
    31021 gaaaatgtcg ttgatttcgc aatgcttgag tctgtaaaag atattgagca actaaaagct
    31081 gacaaagtaa ctttaatcaa aaaaattgca gaggctgatg aggcaatttc taagatgtcg
    31141 caagcaacaa aagatatgca gcttaaagtt gataaagcag ttgtcgagtt aactgactta
    31201 gtgacatcaa gtgtatctag cctttccgtg ccgtctacat cgatggaatc aagctaatgg
    31261 aactaacgtt gaataccgtt ataacactat attatttaat aaaaaaagga gaatttaata
    31321 tgacatatac ttttaaaaaa gatcatctta ttgttgacgt ttggtaccgt cgtgtcgcga
    31381 atggtatttg tgatttcgaa gcagtaccta aattatttaa tttacgagat gttgtgcttg
    31441 agttacttag tcaaaaagtt actgataaag aatcagagtg aggtaactta atgatagatt
    31501 ttgtgcaagt tggcgctttt gcaggtgctg ctttatctat tttgggagta tggggattta
    31561 ttgttaatcc ctttaaaaaa gcaatggaag ctaacgagat tgctatggct cagctcaagg
    31621 actcactcaa agaactagca tacgagctca aaaatataga tagagaccga gagattacaa
    31681 aagctgttat tgatcgtcac gaggagcgtt taggtcgtgt cgaggatgat atcatcatca
    31741 ataaagagca aatcaaaaca ttatttaata aggagaaata agatgattaa ttggaaagta
    31801 agaattaaaa acaaagcatt ttggtcagca attattccag caatattttt agttgtacaa
    31861 gcagttgcaa atgtttttgg ttatacactt gaacttagtg atttaggtaa taaattgtta
    31921 gtagttgtta atagtgggtt ttcagtactt gttattgcag gtattgttac agaccctact
    31981 acacaaggag tatcagatag cagccgtgcg ctatcctaca cagagccaaa ataataggag
    32041 gataaaatga aagcaatcac taaaatagca atggtactag caatagcaat actgtacata
    32101 ccgcttgcag tggttgcttt ttttagttat ccgatttatt tactttttgg aaaggaggag
    32161 taaatggcaa cttatcaaga atataaaagc aggtcaaatg gtaacgctta tgatattgat
    32221 ggttcgttcg gtgcacaatg ctgggacggt tatgcagatt actgtggatt tttaggtgta
    32281 ccgtacgcaa actgcacaaa tacaggatac gcaagagaca tctgggaaca gcgccacgaa
    32341 aatggtatct taaactactt tggcgaagta gagactatgc aagcgggaga tgtcgcgatt
    32401 tttatggttg ttgacggtgt aacaccttac agtcatgtag caatttttga cagcgatgcg
    32461 ggtggaggct atggttggtt tttaggacaa aaccaaggtg gaccaaacgg agcatataat
    32521 ttagtaaaaa taccatattc agcgacttat cctactgcat ttagaccaaa agtttttaaa
    32581 aatgcagtta ctgttacagg taatactggc ttaaataaag gtgattattt tatcgatgta
    32641 tcagcttatc agcaagcaga tttaacaacg acttgtcagc aggcaggtac tacaaaaacg
    32701 attatcaagg tatcggagtc aatagcgtgg ctatctgaca gacatcaaca acaagcaaac
    32761 acaagcgacc caattggcta ttaccacttt ggacgttttg gcggtgatag tgctttagcg
    32821 caacgagaag cagacttatt tttgtctaac ttaccaagca aaaaagtatc atacttagtc
    32881 attgactatg aagattccgc aagcgcagac aagcaagcta acacaaatgc agttattgca
    32941 tttatggata aaatcgctag cgctggttac aagcctgttt actacagcta taagccattt
    33001 acacttaata atgttgatta tcagcagatt attgctaaat acccaaatag tatttggatt
    33061 gcaggctatc ccgattacga ggtgcgctct gaaccactgt gggactattt tccatctatg
    33121 gacggcgtgc gctggtggca atttacaagt gtaggagtag caggtggttt agataaaaat
    33181 attgtattat tagcagatga tagtagcaaa gtggatatac ctaagattga caaaccacaa
    33241 gaaccacaaa gccagcttac ttttaatcaa aagctagata ctaacactaa attagacaac
    33301 tcgaatgtac cttactacga agcgactcta agcacagatt attacgtaga atctaaacca
    33361 aacgcaagta gtacagataa agagtttatc aaagcaggta ctcgtgtgag agtttacgaa
    33421 aaaatcaacg gttggtcacg tattaacgca ccacaatccg aacaatgggt agaagatgct
    33481 tacttaattg atgcaacaga tatgtaaacc aacagagcga cataaatgtc ggtctgttaa
    33541 tggtgtaagt tacaccgcaa ctaaaaaacc agtttaggag gtattatttt attgttaata
    33601 ataaatatta ctgtttttct tgacttagca aataaaaaca ggtaatataa agatacaaat
    33661 aacgacaaaa aactgcgcct ctgttgtttt gtgctcttgt tgtttgtatt gctgtacacc
    33721 tatcgccgta ggtgacgaac aaaaaatgta agaggggact ccaacctcag aaaagcacag
    33781 tttgccggct gtgcttttat tttttgaaca taaaaaagag cgggtttgcc gacccactct
    33841 taaacaaaaa atgtaatgta cgtactattt tttgtgtaaa tagtcacgta cgatatcagc
    33901 taagcaattc gcagtgagag taaccacaaa attgctaact aatgtgagga ggaaaaattc
    33961 catactccaa cctccttttt aaagatttgc tatttgatta gttagctcta gcccatctaa
    34021 gctactagag cttatttgtt gattttatta tatctgattt tgcaatttta aaaaaggtct
    34081 tagctgctaa aaaaaacaag aacttctagt attataagac aaaaccgctc agattaactt
    34141 ctgggctgtt ttttattgca aaaaatttcg catttattga caaaaagttc aagacatgtc
    34201 ataatgaggg tggttataga agataaattt cgttcattta tcatcatttc taacccaacg
    34261 tcttcgttgt tcgttgaacc cgtagtgata cgggcgtatg actgaaagca catcatacgg
    34321 cttggcagag cttaagaact gttctcttgc gataagccta agaagcacaa tagagagtta
    34381 gagttttgca cctctaatcg ttagccctga ccggaggata tttccggtcc gtgctttttt
    34441 attttgtaga aagttttttg atagtggatc tcaaagaaat agttaatgat tatgagctaa
    34501 atttttgtgg gaaaaggtgt aaagttgaga ctaattttaa gcatttaccc gaatttatga
    34561 ttttatttga tataagagat ttgtatcatc ttctaggtat tcataagttg aaaacaaagt
    34621 atcgcgcaac aaattgggtt gaagctgtga aagcagatgt tttcctctta tcgaattatt
    34681 caaaacatcc aaattttaga gaagttcttc ctagagtcga taattataat tttttatatg
    34741 aaatattcta tcagtttaga gttaacgtct gtattttaga taaggattta actaaaaata
    34801 caatgaaatt gagtgttgtt ttttataaag acaacaagaa gaaattagtt gttgtaggat
    34861 taaaaagaga tgagacaggg gtctttaggc cagctacgtt gcatgagagc cgaaacaacc
    34921 cctacaagcg aattcggcat actgctataa aatcaataac ttggatttag aacatacctt
    34981 ttgtgctgat tacctctatt gacacactcg cataatcatg agataatcac agtagcaaga
    35041 atcgcctgaa agtagcagta gatgctgtca aagaaaaact gaaataaata ttaaccgctc
    35101 atttaatgag cggttatttt ttgtctatca gaacagaaaa atttaaaatt gtctattttt
    35161 aggatttttt atcgaataga taagtggagg ataaaatatg ttatatatag atgagtttaa
    35221 agaagcgatt gataaaggct atatttcagg ggacacagta gcgatagtgc gtaaaaacgg
    35281 aaagatattt gattatgtgt taccacacga aaaagtgaga gatgatgaag ttgtggcagt
    35341 agagagtgtg gaagacgtac tcgcagaact gggtaatgtt cgttgtgata taagtagctg
    35401 tcaatagttt gccccaacta acaatatact tttttgtggg gcgaatttgg ggcataagtt
    35461 taaaactaat ctatgttaac aatgatacga cttttatgtt tactactcat aacgacttat
    35521 atagatatgt tttagacctt ataatgccaa ccgaaaaacc taactcttaa agacgctgtt
    35581 aaataattcg tctagaaaaa ccttgtcata tcaatgttta ttgatagcga caaggttctt
    35641 ttttatatcc aatactcaat aaacgactag agagctcaaa ggaatttaga gtttcaaatg
    35701 tgttagagca tacccttgaa ttatcttaac tgtgatgtta taattcctaa aagataagtt
    35761 aaaagagagg ttgcgaaaga gaatgtcaga aaaagctata ctattaatta tccttattgc
    35821 ttttgttatt atcgctatta taaagatagc tctgttaaag ttatatatga atacgataaa
    35881 acctaaagta gataatgaag gaaacatcat aacaaagcct gtaaaggatg atttaacacc
    35941 cgaggaacgt gaagaacgtg aatttgataa agattggtaa aatcaatata aaagagcaaa
    36001 caaggtttgc tcttttatta tttttaaagg taattaagtt gtcctcgaaa atcctccaat
    36061 gattgatagc ctttatcagc cattatttct tttaactctc tgcttacacg ctgaaaaatt
    36121 tctggtcctt ctttttgtaa agcggttccg atttggacca tactagctcc acatagaatg
    36181 tgttcaaagg catcacggcc gtttttaaca ccacctgttc cgataatttt aattgaagga
    36241 tttaaacgtt tgtagaaagc atggacgtta gctaaggcgg taggcttgat aaaatcaccg
    36301 ccgatacctc caaagccatt tttaggcttt ataacgacag tttcatcatc aataacaagt
    36361 ccatttccga tagaattaac acagttaata aaagctaaag gatatttgtt aaaaattgta
    36421 gctgcttgat caaagtgaac gatatcaaag taaggtggta atttgatgcc caatggcttt
    36481 tgataatagc taaagatttc tgaaaggatg aggtcagtca tttcaaaatc ataagcaatt
    36541 tgaggttttc caggaacatt tgggcacgat aaatttaact ctatcaggcc attgtaacta
    36601 ctgttttcaa cctttttaag aataatatgt gtctcttctg gtgataaacc tactaatgat
    36661 aaaacgtgat ttttgctatt gtcttggtct tgtaattcag tcacgaaatc aagataatag
    36721 tcaatacctt tattagggag tcccatagaa ttaattgatc cccagtcagt atctgcataa
    36781 cgtggttgag ggtttccctc tctagcttta agggtacctg tttttgtaac aaaggaacca
    36841 gcttcagaat tttcaatagc taataactct tccttagtca tacaataaat acccgctgca
    36901 ttcatcaaac aattgtcaaa actaaaacca gcaatttccg tttttagcga taccataata
    36961 tcctcctgaa gataaatttt tactatttta tcacttgatt aacagagcgg caagaaaata
    37021 atgatattgg taataaaatt tcgctaaata acgaacgtta ttatttgtgt aaacaataat
    37081 agtaaagaaa taaatgttgc tttctataac gcataaaaaa ataagcttat gtaagcttat
    37141 tttttatgtt cggcgtaaga ttttctttag taattgaata ctcttatatt ttaatggatt
    37201 tggatagtag cggaaagtac ccattttacg aacaatgtaa ccattaaaat tctgtttaaa
    37261 gcgaagtacg ccatctgaac catcaaaatt tccttgaata cctaaaaaat tatagaaagt
    37321 tgattgacgc tttagagcct cttgcataac atattcttga agaactgcag gcgcataaaa
    37381 tttattaaat tctgtgtatg agccagagaa aagatagaca gtttctttag ggctataaat
    37441 aaagaggcta ccagctaaaa caacatcttg atttccaaat ttttgaataa aaggttcgac
    37501 ttctttgcga cgtttatcta aactagctag ttgattgaca agctcagtac gttgacgatg
    37561 gtatttggca gaatctgtat tattttgatg gcgattatca aggactgtaa tctgttcttc
    37621 caatttcttt gcatttaatt gtaggttatg atcatattcc ctaaagttta gagttgcaat
    37681 gacaaattcc gccttatctc cgaaactatc ataaaaatct tgataataat ctagagattt
    37741 atccatatag tcccggcgat cagaagtaga agaagtaata tccttaaaga tatgtagttc
    37801 ttcccgcttg aggacacgta tttttatacc aaaagacatg gctttcttaa ccaaggggcg
    37861 tcctttttta gagaaagatt taagaagatt ttgaggagtg ataccttcta gatttttaac
    37921 ataatgccaa tcaggttcac cgccaggata accaatatga agcccatcgt ggtgataacc
    37981 tatactagtc aggccatcaa taagataggt gttcggtgca cctttaggtt caccttcacc
    38041 agtaaactct tggtaggtat cataaggttt aattaataat tctaaagcgc cctgagattt
    38101 ggcatagttc tgtagttctt tataaaaatg tttcagatat ttagaattgg aatgggctgg
    38161 accagaattg acttccatat gaagtccacc agtcattggg atggtataaa caatactgat
    38221 gatttccatt ttcccatcga tttggtagcc catgtaggta atgtcatacc cacgtttttt
    38281 tagaagcttt gccatctcag gggtttgcat aaacgattgt aaatcgtaat ttcctgaata
    38341 actttcgaac tctttagctg ttaattcttt aagcgccata aaagtatcct ttcctaacta
    38401 gctattacgt tttttgcgta aggcatagac ataattagag gctttataaa gcaatctgtt
    38461 cactggaatg ttaaattctc caataaattc ctcaatgatt ggttcgaact tagacttaaa
    38521 atgatataaa ccaccagata agtcattctc aatccccccc atattttgcc aaatatttcc
    38581 acgttcaaaa gccctttgag ctgtttcaaa ccaagtgtaa ataggagcag aataagattt
    38641 gaagtaatcg tccataccag cataaatatt ttctgatgta ttaccaaatt ctagagtgag
    38701 ggttgcagcc aatggaacaa tgtctcggtc ataggctttt tgagcttctt tgagaaaatc
    38761 aatttcttta tttaatcgat caatcgtatc ttggtgttgt ttaacttttt tctcacgatt
    38821 aagttcaagt gattttattc tttcagattg agctatctgg cattcttttt ctattttttc
    38881 taatcgtttt gcaacatcta gcgaagccat tgtgatgtat gagttattag gatacgtatc
    38941 aaggagtttt tggtagtagt caattcctct aagatttatc cctttacggt cttctgtttt
    39001 tttcattaac tctgcaaaat cttcaagtag ttcatggcta ccaaattgta tatctactcc
    39061 cttattttta gaagttcgaa tagcttgacg agttttttta gacattttat caagtccaaa
    39121 ttgattagca tataggtttg cttgaaaacg tggttggata gtatgagcta aatctgttgt
    39181 ccgaccagtc caatcagcac ctgcacgctg taaatgatca atggcgatta acccttcttt
    39241 ttcatcgggc gaattttcaa aatctttggc attgaccatt tttaaataga tgagaggatc
    39301 acacttgata aagagagctc tttgtttttt gccaaaagct ttaaggtcct taaggacctt
    39361 agttacaata tctagattgg agtaatccat gattggtcca cgcggaatat acagcatgga
    39421 gaagccaaga ggaagtgatt tgatgagaat actagcgctg gcaatttggg tttcaccgtc
    39481 aaaaaagcca agtaattgac taccccagtt gtcttttact ttgggccaat tagaagattg
    39541 aagtaggttt gtttggttgg attcgctcac aaagcgatcg tgttcgacag cggtaatttc
    39601 tctatacatg agtttccttt cttagtgaga gtgtctttgt ttttttcgaa tagtataggc
    39661 taggttggcc aatttatata ataatggatc gattgctaga ttaaactcgc caatatattc
    39721 ttcaaacatt ggattgaagt tacttttaaa ttttgttaaa ccatcgttaa gacttccctc
    39781 gacaccgcct agattagcag atacaatacc gtttttgaaa gcatcttcga acaccctagc
    39841 attcaagaga tactgtggat aaaatttacg gaagctatca tccataccag catacaacat
    39901 ctccaggaca ttgccaaaac gaatggacaa aattcctgca atgacaggct catctggata
    39961 tttcttgcta aactcctgaa attcaaggat atattgttta acagaagctt cttgttgatt
    40021 tagccttgtc aggcgacttt tttgatggga tggagtttct gataaatctc tttggatttg
    40081 aagaagttgt tctttaaatt gcgctaatct tttaggaaga ttgactttag ccaaatagag
    40141 ataggcatct tctccataaa ttgtcattag ttgatggaaa tagttttcat tacgtaaagc
    40201 tacaccttta cgattctcgg ttaacaaccg tggcgaattt aggtaaatca tcaatgttgg
    40261 cacgataaat ttgaacaccg cgatgctttg catcttttat cagacgttta gtgtgttttg
    40321 gaaagttttc ttctatattt gctttggtat agatgttcgc ttgaaaacgt ggttggatgg
    40381 tttcggaaat ggctttcgtt ggacctatcc attgggcacc tgctgactta agattatcaa
    40441 ttgcctgtct actttcatct atctcttcgg cgacattctc ttctttaagg tgatattgac
    40501 gtagaagaag tgctggatcg aattttgcaa aaacagcacg tttctttcgt ccaaagtttt
    40561 ttagtgtttt taacacaaag ccaactaatt ctttattgct gtagtctata ataggtccac
    40621 gtggaatata aagcattgta aaaccaagag gtaatgattt aattaagatg ctagctacag
    40681 caactaattg gtcatcttta taaaagccta aacgttcatt ttgccaattt gattttactt
    40741 gtgcccactt tgaagagtgg aggagattgg tttgagacga tgcaatagca aaggcatcat
    40801 attctttttc ggatattcca acggtaacat gatacatcag tttattaccc attcacgtag
    40861 tgcgtaggct acgccacttt cctcgtttga tttggtgata tattttgcta ttttttttag
    40921 ttcaggattg ccgttttgca taacaacagg atttccaaca acttcaagca tagcacggtc
    40981 attttcttca tcaccaatag ccattgtttg atctacagtt aatcccattt tctctgcaag
    41041 gtggagaagt gcagtgcctt tattaacgtt tttgggtaaa atttctaaat aaaaaggagt
    41101 tgattttaca atggtgtagt tatcgagaac tttttttgga attttagcaa ttgctgcatc
    41161 taagatgtca ggttgatcaa tcatcattag tttaatgatt tctttgtctc ccatttcttc
    41221 aggagtacga tagaagagcg gcatattaac taaagttgct tcgtgtattg tatatttacc
    41281 aatatcacga ttcgctgtgt aaattccttc ttttgtaatg gcgtgcatgt ggacgcctaa
    41341 tttacgcgct aacagttcga aatccagata gtcttcatag gttaaggttt ctttgatgat
    41401 gtcatctcct gtggctgtgt cttgaactag accgccgtta aaggtgataa cataatctcc
    41461 ctcttcattg agtttcaatt cttctaaaag atcttggact ccgatgattg gacgtcctgt
    41521 agcaataaca attttaacgt cttttgattt tgcttcttga acagctttag ccacttcagg
    41581 tgtaatttca cgcttactat ttaatagggt gccgtcaata tctacggcaa caagtttaat
    41641 tgaagtcatt ttatctccca ctctttattt caatatccta aaatactcac cttatatctt
    41701 actaaaaaat aaagggtttg tcatgaatgg ctattatctt tcgtgagtgt caaatgctca
    41761 tttgtgatat aagattgaaa ttcttctttt gtacttgaaa ataggctatc taatgtcaac
    41821 atttcttttg gaaaataaaa acgttgatca ccataattag atccagttaa tgcttttaca
    41881 atcggtgata agcttgataa ttcagcaagt tgtccattct tttggataat ttgaatttct
    41941 gttcgtggat ttgaatgttc aggccgataa acatcgtaag gtaagtcaaa attagcatgg
    42001 acaccagtgt aataatcggg atcaaaacca acctgtgaga ctagttcttt cattttaact
    42061 agattttctt tatcagcctc ttcaaaggta attgatttaa agacttttcg attgataaaa
    42121 cgattagcta aatctgccaa aatgttatca tcagcttgca tccaagattg gaagtaagta
    42181 ttcattaccc catcatctag ggctaaataa tcttgtaaat catattgatc cgtgaagaaa
    42241 ggaataagat ttggagaagt ttgttcaaaa aaatcgcgat gggtatcaaa taagaagcga
    42301 gcacgtttta gtaaattttg taagagtaat tccatagcac gactggctgg atggaaataa
    42361 acttgcatat acatttggaa gcgactaaca atataatctt ccacagcgtg catcccgtta
    42421 cgagcaaaag caattccaga atcggttggt ctaataacgc gtaaaattcg tgttaaatca
    42481 aattgtccat agctagcagc agtgtagtaa gaatctctga gaagatagtc cattcgatca
    42541 caatcaattt gactagaaat taattgaaca acttgtttat tggggtagct atgattaata
    42601 acactagcta ctttatcagg aaaatcagga gccacctttc gtaaaatagc attaatctct
    42661 gtggtaggat tggtgatgat ttcctgagtg taagcttcat gatcagtatt gaagagacgc
    42721 tcaaatgtat gagaatatgc gccatgtccg atatcatgta gtagggcggc agccatcgtt
    42781 agcagggact catttttatt ccaaagatca gaataatgtt catcaaagat ttctgtaact
    42841 ttacgagcca gttcataaac tccgagacaa tgtgaaaaac gactatgctc tgcaccatgg
    42901 aaggtaaaag aagtcgttga ggtttgttta atgcgtcgta aacgttgaaa ttctttagtg
    42961 ttaatcaagt catatatgac ttgattattt acatggatat aagtgtgaac ggggtcacga
    43021 aatacttttt cattcatata tatattatag caaaaattga aacttgagtc agaaattggt
    43081 agaatagaag gataaaagaa gtgagagaag tcatgaaact agaaattaac aataacattc
    43141 aaattgacaa cgaaaccgaa atgattcatg agattcacga ttgtcaattt attgaaaaag
    43201 gaagttacgt ttatttgaat tatattaatg ctgagggcga gagagtagtt attaaggcaa
    43261 atcatgagga attgcttatg acgaggtttt caaatcctaa gtctgtaatg cgttttcatc
    43321 gtgaaacgcc tgctttggtt aatatcccca ctccacttgg tattcaacat ttgattacag
    43381 agacaagtca ctatcagttt gatttgtcac agcaaaggtt gcacatcaat tatgtcctta
    43441 agcaaacgga gacaggagcc tgttttgcaa attatgaatt aaagattcaa tggtattgag
    43501 ataattacca ttaacaaaag agtagtcatt atgaatttcg tctactgaag acgctgggat
    43561 ctctttatga gagccattta tttgataaac tgcagtatgg tttatttcga ctagatgaac
    43621 ttcgtttttt cccttgagat tattagtgat agcaaggata attaattgaa aaatggattt
    43681 ttttctgtta caatgattaa catatcaagg gagtagcaga cggtttaccg tggcaatgtc
    43741 gtcattacga aagaaatttc cggcgttgtc ttaaacagcg agacttgttt tcataaaaac
    43801 aggtctcttt ttgtttgtgg agagaccaaa ggaggaaagg ttttgttaaa agaacagaaa
    43861 aaatccttgt tttacacaca aggtcaagaa gaggttctaa cgtctttaga atcttctcgt
    43921 gaaggattat caacaacaga agcgaaaaat cgcttagaga tgtatggtcg caatgagctt
    43981 gaagaaggga aaaaacgtag tctaatagct aagttttttg accaattcaa agatttaatg
    44041 attattatct tactggttgc tgcagctctc tctgttatta cggaaggtat gcatggctta
    44101 acggatgcat tgattatctt ggcagttgtt atcttaaatg cagcttttgg tgtttatcaa
    44161 gaggggcaag ccgaagcagc tattgaagcc ctcaaagata tgtcaagccc aattgcgcgt
    44221 gttcgtcgtg atggtcatac tattgaggtg gattctaaag aactagttcc tggtgacctc
    44281 gttatgttag aagcaggaga tgttgttccc gcagatttac gacttttaga agccgcgtct
    44341 ttaaaaattg aagaagcagc tctaactggt gagtcagtac ctgttgagaa agacatatct
    44401 caagtagtag cagaggatgc aggtattggt gatcgtgtga atatggcgta tcaaaattca
    44461 aatgtcacgt atggtcgagg atatggtgtt gttactaaca cagggatgta tactgaagtt
    44521 ggtaaaattg ctgatatgtt ggcaaatgct gatgaatctg aaacaccatt gaaacaaagt
    44581 ttagttcaac tttctaaatt attgacttac ttaatcgtga ttattgcggt tattactttc
    44641 ttagtgggga tatttgtccg taaagaaggt tggattgaag gattgatgac ttcagttgct
    44701 ttagcagttg cagctattcc agaagggctt cctgcaattg ttacgattgt cttatcaatg
    44761 ggaacaaaaa ctcttgctaa acgtaattct atcgttcgta aactccctgc agttgaaact
    44821 ttaggttcta cagaaatcat tgcatcggat aagactggta ctctgacaat gaatcaaatg
    44881 actgtcgaaa aagtgtatac taatggcgtt ctccaatcat cttcggaaga aatttcagtt
    44941 gataataaca ctttacgtat tatgaatttc tccaacgata ctaagattga tccttcagga
    45001 aaattaatcg gtgacccaac agagactgca ctggtacaat ttggtttaga taagaatttt
    45061 gatgtccgtg aagttttgaa aaatgaacca cgtgtcgctg agttaccatt tgattcagat
    45121 cgtaagttaa tgtctacgat tcataaagaa tcagatggtc gctactttat cgcagtcaag
    45181 ggtgctcctg atcaattatt aaaacgagtt actaaaattg aagataatgg cttggttcgt
    45241 gatatcacgg ctgaagataa agaggctatc ctaaatacca ataaagaact cgcaaaacaa
    45301 gctcttcgtg tcttgatgat ggcttataaa tatgaaacac aaattccaac tttagagact
    45361 gatattgttg agtcagattt agtattctca ggtttagtgg gaatgattga ccctgagcgt
    45421 ccagaagcgg cagaagctgt tcgagttgct aaagaagcag gaattcgtcc gattatgatt
    45481 accggggacc atcaagatac tgcagaagct attgcaaagc gtctaggtat tattgatgca
    45541 aatgacacag aagatcatgt ctttacgggt gctgaactta atgaattaag tgacgaagag
    45601 tttcaaaaag tatttaaaca atattctgtc tatgcacgtg tttctcctga acataaagtg
    45661 cgtatcgtga aagcttggca aaacgatggc aaagtcgttg ccatgactgg tgatggtgtt
    45721 aatgatgctc catcgctaaa aacagcagat attggtattg gtatgggaat aactggtaca
    45781 gaagtttcca aaggtgcttc tgatatggtt cttgctgatg ataattttgc aactattatt
    45841 gtggctgttg aggaaggacg taaagtcttc tcaaatatcc aaaaatctat tcaatatctt
    45901 ctttcagcca acatggcaga ggtatttaca attttctttg caactttatt aggatgggat
    45961 gtacttgcac cggttcacct tctttggatt aacttggtaa cagatactct tccagctatc
    46021 gctcttggag ttgagccagc tgaaccagga gttatgacac ataaacctcg tggtcgccag
    46081 tcaaacttct ttgatggtgg tgttatggga gctattatct atcaaggtat tttacaaact
    46141 atcttagtac ttggtgtcta tggttgggca ttgatgtatc cagaacatgc tggttatcgc
    46201 atgattcacg cagatgccct tacgatggcc tttgcaacgc ttggtttaat ccaattggta
    46261 catgcattta acgttaaatc agtttaccaa tcaatcttta cagttggagc ttttaaaaat
    46321 agaaccttta actggtctat tccagtagct ttcattcttt tgatggtgac tattgttgtt
    46381 cctggattta ataaactttt ccatgtaact catttaagtt caacacaatg gttaactgta
    46441 gttatcggaa gtttattgat ggtagtgtta acggaaattg ttaaatttat ccaacgtaaa
    46501 ttaggacaag acgaaaaagc tatttagttt agctgataaa aaaactcact tagaatatct
    46561 aagtgagttt tttagcaatc aaatattgtc atagagttgc gtaattcgtc ttcaaaatat
    46621 ttttttggat agttttcaaa actatccaaa tgcttaacgg cattatgcct tttgctaaga
    46681 ttccaggtta aattagggtt atgattggga ttctgattga caaagtcaat agttaaattc
    46741 cattctctaa tatatccaag tggtctagaa tgataagtag tctctccaat tttaacgtcg
    46801 ccaaatgagc ggtgtgcatg accaaaaaca acatctttga tatgatattt ttggaataaa
    46861 tcatgataag cctgactccc caaaaaagca ttaaaaggag agaatctagg atgagtcatc
    46921 gtgaactgtt tatggggaac aaagtgcata gcaataatga tatttgaaga atcgactttt
    46981 gctaatattt cgtctaagcg ttttaaaatt gatgcttgga tagttacatc attatttgga
    47041 cgttttaaac gtctatcaaa ccaaaaagtc ttttttaatt tttcaacatc cttgatatct
    47101 ctattatttg aaaagctgta atcatactag ccatgaaaag ctagtagcat ttttttgtca
    47161 aatcgatagg tttgaaagtc aaggcgttgg atttctgttt ctgttaaatc aagcatatca
    47221 tggtttccta aattatagct aagtttaata tgctttttta agttattgat aaagggaagt
    47281 gtatctttag taaaatgatt agatatatcc ccagcgatat ggaggtgatc aattttttct
    47341 ttcttaagca aattaattaa cgttttcgtc tcaaaggtag aaaaatgatt gaggtcaata
    47401 tgtaaatcac tcatgatagc aagtttggtc ataagggtat tgtaccatat tgtagttcaa
    47461 agagataaac agtaaatttt tgaaatagtt ttaaagtcat tctataatag atatgtcatt
    47521 gtagaatgcg ctttcataat tgtgtttaga agctttatat ttaaggtgcc ttaaggaggt
    47581 ctcatgtcta atttttataa gctcttgaaa gaaaaatttc ctcgaaaaga agatatcgta
    47641 acagaaatga tcaatttaga agctatttgt caattaccca aagggacgga atattttatt
    47701 agtgatttac acggggaata cgatgctgtt gattatctct tacggacagg tgcagggtct
    47761 attagagcta aattattaga ctgctttgat tggcagaaaa tagttgctgt tgatttagat
    47821 gatttttgta tactgcttta ttacccaaag gaaaaattag ccttcgataa aatgaacttg
    47881 tcagtaagtg cttataaaac gaagttatgg gaaatgattc cgttacaaat acaagtttta
    47941 aaatattttt ctagtaaata tacaaaatct aaagtacgta agcaattgcc aggtaagttt
    48001 gcttacatta tcgaagaact tttagctgag attgaccgta acccagagaa gaaaagctat
    48061 tttgatacta ttattgaaaa attatttgaa ttggaccaag tagaagattt aattatagta
    48121 ttatcccaaa caattcaagt tttaatcatt gaccatttac atgttgtagg ggatatttac
    48181 gatcgtggta ggtatcctga tcgtatcttg aataggttaa tggcttttcc aaatttggat
    48241 atccaatggg ggaaccatga tgtcacttgg atgggagcag catctggttc ctacctttgt
    48301 atggtaaatg ttatccgtat tgctgcacgt tataataata ttactttaat tgaagaccga
    48361 tatggtatca atttgcgtcg cttggttgat tactcacgcc gttactatga acctctacct
    48421 tctttcgccc ccatattaga tggagaggaa atgactcatc cagatgaatt agatttgtta
    48481 aatatgatac aacaagcaac agcaatttta cagtttaaat tggaagccca attgattgac
    48541 aggcgtccag aatttcaaat gcataatcga caacttatta accaggtaaa ttataaagat
    48601 ttaagtatat ctataaaggg agttgtgcat caacttaaag attttaatag tagatgcatt
    48661 gattcgaaaa atcccagtcg attaacaagt gaggaagaag agcttttaca acaattgatg
    48721 atagccttcc agacttctga aagtttaaag aaacatattg atttcctatt tgagaaagga
    48781 agtatgtatt taacctataa tgataatctt ttattccatg gttgcatacc aatgcatagt
    48841 aatggtgatt ttaaaagctt taaaattgca ggaaaaactt atggtggtag ggatttactg
    48901 gatttatttg aaagtcaaat acgtctagca tatgctagac cagagaaaca tgatgatttg
    48961 gcgacagata tcgtttggta tctttggtgt ggtgaaaatt cttctctttt tggtaagaat
    49021 gccatgacta cttttgagcg ctattatgtt tccgacaaag taactcatca agaaagaaaa
    49081 aatccctact ttaaactgag agataaagat gatatttgca ctgccctttt acaagagttt
    49141 gatttgccta agtttggaca tattgtcaat ggtcatacgc cagtcaaaga aaaaaatggt
    49201 gagcaaccga taaaagcaaa tggtaagatg ttagtaattg atggtggctt tgccaaaggt
    49261 tatcagaaaa atacaggttt agctggctac actttgattt ataatagcta tgggatacaa
    49321 ttaatttcac accttccttt tacatccatt gaggaagttt taagtggaac caactatatc
    49381 attgatacga agcgattagt agaagaagcc aaggatagga ttcttgtaaa agatacaact
    49441 attggacaaa agttaaccaa agagattaaa gacttagatc acctttatcg tcattttcag
    49501 gaatatgatg actagtgttt aagtaattta ctaattgata tgtaggattt aatttttcag
    49561 tttcttacct tgtctctttt attaaattga cttaaaataa tattatggac ataaaggcag
    49621 aaattcaaaa attagcaaaa gaaattggta tctctaaaat tggttttacg acggcagata
    49681 actttgatta tttggaaaag tcattgcgtg cttctgtgga agaggggcgc aattcaggat
    49741 ttgaacataa agtcatagag gatcgtatct accctgaaag gcttttagaa tcggctaaaa
    49801 cgattatttc aattggcgtt gcttatcctc acaaacttcc tcagcaacct caaaaaacac
    49861 cttataaacg aggaaaaatt acacctaatt cgtgggggct tgattatcat tatgtggtcg
    49921 gcgaaaagtt agacagacta tcaaaaggca ttgaagagct ctgtcgagat tttcctttgc
    49981 aacaaaaagc aatggtcgat actggagctt tagttgatac tgcagttgcc caaagagcag
    50041 gtattggttt tataggaaaa aatggtctag ttatttctaa agaatatggc tcttatatgt
    50101 ttttgggaga attaattacc aatttagaga tcgagccaga caagcctgtc gattatgatt
    50161 gtggtgactg tcgtcgttgt ctagatgcct gtccaacttc ttgtttaatt ggtgatggct
    50221 caatgaatgc caagcgttgt ctatcatttc aaacccaaga taagggaatg atggacatag
    50281 agtttcgaaa aaagattaaa acagttatct atggttgcga tatttgccaa atttgctgtc
    50341 cttataacaa aggtataaat aatccattgg cgacagagat tgatcctgag ttggctcaac
    50401 cagaattaat accatttttg agtttatcaa atggacaatt taaagaaaaa tttggtatga
    50461 ttgctggaag ttggcgaggt aaaaatattt tacagcgtaa tgccattatt gcgctagcaa
    50521 atgctcatga taaaaccgca gtggttaaat taatagagat tattgataaa aataataacc
    50581 ctattcatac tgcaacagct atatgggcat tgggagaaat tgtcaaaaaa ccaaacgatg
    50641 agatactgga gtttatgagt aatttaacct taaaagatga agatagccga aaagagttgg
    50701 aattaatccg tcataaatgg caattttaag ccttttatgg tatttttaag aaccttatga
    50761 taaaataaaa cagattaaca aagattggag aaatctcatg gaagtggctg aaattcgcca
    50821 aaaaatagta gaaaataaag agaagttgac tagcttcagg aggtctcttt gacttagatc
    50881 gtttggaaga ggagattgct cttcttgaaa atcaaatgac tgagccagac ttttggaatg
    50941 acaatattgc agcgcaaaag acatctcaag aactgaatga gttaaaaggt aagtatgata
    51001 ctttccataa tatgcaagaa ttatctgacg aaacagaact cttacttgag atgcttgacg
    51061 aagatgattc tttgaaagaa gaattagaag aaaatctaat gcagctagat aagataatgg
    51121 gtgcttatga aatgacgctt ctcttatctg agccttatga ccacaataat gctatcttag
    51181 aaatccatcc tggttcagga ggaactgaag cgcaagattg gggagattta ctcttacgaa
    51241 tgtatacccg ttttggcaat gctaatggtt tcaaagtaga agtattggat tatcaagcag
    51301 gggatgaagc tggtattaaa tcggttaccc tctcttttga aggtcccaat gcttatggtt
    51361 tgttgaaatc agaaatggga gttcatcgcc tcgttcgtat atcacctttt gattcggcta
    51421 aacgtcgtca tacctcattt gcttcagttg aagtgatgcc agagttagat gatacgattg
    51481 aagtagaagt acgtgatgat gacattaaaa tggatacttt ccgttccgga ggagctggtg
    51541 gtcagaacgt caataaagta tcaacaggtg ttcgtttgac gcatattcca actggaattg
    51601 tggtatcgtc taccgttgac cgtactcaat atgggaaccg cgatcgcgct atgaagatgt
    51661 tgcaggctaa gctatatcag ttagaacaag aaaagaaagc acaagaagta gatgctctca
    51721 agggtgataa aaaagaaatc acttggggaa gtcagattcg ttcttatgtt ttcacccctt
    51781 atacgatggt gaaggatcat cgtactaact ttgagcttgc tcaggtagat aaggttatgg
    51841 atggagaaat caatggcttt attgatgcct accttaagtg gcgcattgaa gactaaaaat
    51901 aatgaaggag aaacatggca ctaatagaaa tgtctggtgt tactaaaaag tatcgccgtt
    51961 caacaactgc cttgagaaac ttaaatttat caattcagca aggtgagttt gtgtatttag
    52021 ttggtccgtc aggtgctggt aaatctagct tgattcgcct cctataccgt gaagaaaaat
    52081 taagttcagg tagattaaag gtaggggaat tcaatctcaa taaactcaaa cgtcgtcaaa
    52141 ttcctatttt acgtcgtagt attggagttg tcttccaaga ttataaatta ctaccaacaa
    52201 aaacagttta tgagaatgtt gcgtttgcga tgcaagttat tggagctaag cgccgtcata
    52261 ttaaaaaacg cgtgccagaa gttttagaat tagtaggttt aaaacacaag atgcgttctt
    52321 ttccaacaca gttatctggt ggagaacaac aacgtgtggc aattgcacgt gcgattgtta
    52381 ataatccaaa attattaatt gcagatgaac ctactggtaa tttggatcca gaaatcgctt
    52441 gggaaatcat gcatctattg gaacgtatta accttcaagg aacgactgtt ttgatggcaa
    52501 cacacaatag tcaaattgtt aacacacttc gtcatcgtgt cattgagatt gaagcaggta
    52561 gcgttatccg agatgaagag aagggagaat atggttatca tgattaattt ctttagacat
    52621 ttctgggaat cgcttaaaaa cttaaaacgt aacttttgga tgacttttgc ttctgtaaca
    52681 tcagttacca taacattact tcttgtaggc ttgttttcat cagttttact gaatgttgaa
    52741 aagttaacaa ctgatgtttc aggtaatttt actatcagtg cctttttaaa tgttgactca
    52801 acggatgctc aaaaacaagt caaagataag gacggaaagc taaaagataa tcctgattat
    52861 cataaagttt atgacaagat taaacgtatt tctggtgtag agaaagtcac ttattcaagt
    52921 aaagctgaac aattaaaaga agttcaaaaa gagtatggtt cagatgttat tgatgatacc
    52981 tacaaagatg ctttgttaga tgtttacgta gttgggacaa gttctgcaaa agtttctaag
    53041 agtgtcagtg aagcaattgg acgcattgaa ggtgttgatt atactaaaga acctattgat
    53101 tcaacaaaac tatctaatct cacagataac atccgtatat ggggatttgg tggtgttgcc
    53161 ttattaattg tccttgctat ctttttgatt tcaaatacta ttcgtatgtc gattatgtca
    53221 cgccgtacag atattgaaat catgagatta gttggcgcta aaaattctta tattcgagga
    53281 ccgttctttt ttgaaggtgc ttgggtaggg attttagggg ctatcgttcc atcgctgatt
    53341 ttctattttg gttatcaatt tgtgtttaac aagtttaatc caaaatttga gacaagccat
    53401 gtttcattgt atcctatgga tattatggtt ccagcaatta ttggtggtat ggtgattatt
    53461 ggtattatta ttggctcact tggatctgtc ctatcaatga gacgttacct taagatttaa
    53521 taaaaagaga tgaaatc
//
LOCUS       DATROQ010000003        48845 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.003, whole genome shotgun sequence.
ACCESSION   DATROQ010000003 DATROQ010000000
VERSION     DATROQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48845
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.003"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            278..670
                     /locus_tag="VBC94_000114"
     CDS             278..670
                     /locus_tag="VBC94_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEO6978424.1"
                     /translation="MQSQKLNILQRVLRDKFLLQGLGVPNSNTVLNRSFTALLLALLL
                     EDSNNKSWISEEDQIKIMNQAFSWLIDELDFRGFDEELGWIHVFAHGADLLTEIVKMK
                     CFSKDDSSKILTIIKRVMITDDILTLNG"
     gene            849..1703
                     /locus_tag="VBC94_000115"
     CDS             849..1703
                     /locus_tag="VBC94_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HEO6978425.1"
                     /translation="MRDEQEIYNLVLNIANQDKRIEAVLLNGSRANPNVPKDDFQDYD
                     IVFVTNFIEDIISDTNYHKKFGDILIMQKPNEFRNKTEYNCFAYLMQFQDLTRIDLRL
                     IKPEFLEDYLDDAFSKVLLDKKNKYLDYNFERSSLYETKQLSEDEINKILNEIYWVST
                     YVVKGIARNDIMYSEFMISNPIKNAFIKLLKQKILIEKELDSLSFGKLDKDILQYITD
                     KDQLLKIFSNKSLKDIESNLRFLLDETNQMAKYISINRKLNLNQGEYQSAMKFMNIFL
                     SNSYQNFN"
     gene            1966..2262
                     /locus_tag="VBC94_000116"
     CDS             1966..2262
                     /locus_tag="VBC94_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978426.1"
                     /translation="MTEKNYNNHTISLNKTEFFTITDDQVTYTKNEELEKISYDSKNN
                     ELLLNGKKFWTHLTSGELQLTDPKNTDMTLNYASKNSPLFKSYEKGTAKFKEEN"
     gene            2522..2854
                     /locus_tag="VBC94_000117"
     CDS             2522..2854
                     /locus_tag="VBC94_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978427.1"
                     /translation="MKNLFATTEASSRKQEQDRIVNYIKQHVELTNGNQIKKIEFIDF
                     QKNEMTGTWGISTKINEQFSISFSEDRIGGKLRALGYQPNEIGFSKDINSNNQNVNDI
                     EVIYMKKE"
     gene            2856..3365
                     /locus_tag="VBC94_000118"
     CDS             2856..3365
                     /locus_tag="VBC94_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978428.1"
                     /translation="MISEESYRYLVEDAYRVDSKKVKIPLKRSDIVGNSDYVIIEPPI
                     DNTSNGMQAMVVAPIKEGMTAEPDTSEIVIVYAGTNLGERLDIATDVEMVAGGDTYLL
                     ADPKTKTFRKSQGKSALEYAEKISSKYPNSEITTTGHSLGESEALYVALKMGWMNVGY
                     NGPDIHNMI"
     gene            3536..3922
                     /locus_tag="VBC94_000119"
     CDS             3536..3922
                     /locus_tag="VBC94_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 family protein"
                     /protein_id="HEO6978429.1"
                     /translation="MKKKYWILLILLLTVLSGGGYMVFKQHTEKEKMIAIAHSAEAMQ
                     IYEKHMKANDSRAFSEQGIIHNYKINDSELNYNPMGGMEVTVYVNNDTNKYFQFTILD
                     NGNGKLESGSYIISADLDNDLETNKK"
     gene            3959..5176
                     /locus_tag="VBC94_000120"
     CDS             3959..5176
                     /locus_tag="VBC94_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6978430.1"
                     /translation="MSEYKTIHPNARLKDQYDNTIGVVSEVYDNTSHSGEQIYAVVDN
                     RHIPKEDVKEVTVLFRGSTSPQEFINNPADVTLDWLENDIPMASNIWVMKDFGNPHNF
                     SAVSPQLKASSEHLKEIMKKYPNAKINIGGHSLGGMDAQYAVVDITDKKDLKRINSVH
                     VYNAPDIYPTLTKGQKKTADSLKSKIVVYVDPNDFIGMVGREGKKGSEDSVGTVYYTE
                     SPDINWIDQHMTYGYRMENGQIKVIETNLPPEVRTTRKAMGSFYKDKKKFKKSGKGLS
                     SHEKIFLDAEQAAVIASGLVSTAETALEDIKTSAKTAVGEAEELWNTTKSIPFGITEL
                     TEAELAEAYAEGGVTYDSIVTKTDTHFDEKVKNAGDLVTTYTTLKSDIQSGVETMLSK
                     DSELAGDFKAWEN"
     gene            5164..5565
                     /locus_tag="VBC94_000121"
     CDS             5164..5565
                     /locus_tag="VBC94_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978431.1"
                     /translation="MGKLDAKDDKLSNSKKDLEKLEDDYHHKTIAISDKFFELEDKRC
                     EFETMLQETYEATSYHLRQDEMINEESFMTMNHIIEAFQSDFDTEYTKEKRRLTALEE
                     ETNQKYSKKRQLLEEKIDHLMSERRDYGNPW"
     gene            5549..5854
                     /locus_tag="VBC94_000122"
     CDS             5549..5854
                     /locus_tag="VBC94_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978432.1"
                     /translation="MGILGDYVLSEASDYISGKKDIKALKKKLDKMLVTGVYAPRIKS
                     KRRSIVSTYDAEITTTATNAKASITAIAGQIDTAIKGQFRTKVETVLDNNSTKYDEI"
     gene            complement(6043..6115)
                     /locus_tag="VBC94_000123"
     tRNA            complement(6043..6115)
                     /locus_tag="VBC94_000123"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6080..6082),aa:Lys,seq:ctt)
     gene            complement(6397..7665)
                     /locus_tag="VBC94_000124"
     CDS             complement(6397..7665)
                     /locus_tag="VBC94_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEO6978433.1"
                     /translation="MADNLALRMRPRNINEVIGQQHLVGNGKIIDRMVAANMLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGNIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKKAIQ
                     LAISDKERGFPFLVTIDDGALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDGSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPEAQIHTVTALEAAQRIGFPEARILIANIVVDLALSPKSNSAYL
                     AMDAALADLRRSGNLPIPRHLRDGHYSGSKTLGNARDYKYPHAYPEKWVKQQYLPDKL
                     VGHNYFEANETGKYERALGSNKERIDKLSD"
     gene            complement(7658..8149)
                     /locus_tag="VBC94_000125"
     CDS             complement(7658..8149)
                     /locus_tag="VBC94_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001879696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6978434.1"
                     /translation="MIIRPMQNCDREAVLTLQNTGWTALTSPVYDHKWTESDLEKNLA
                     NGMSFFVAEIDDKIAGVLDFGPYYPFPAGKHVATFGILIAEPYQGQGLGKALLKALLT
                     EAKAQGYIKIAMHVMGNNSRAISLYQKYGFTEEARITKAFFIENHYVDALIFAKDLEN
                     NHG"
     gene            8476..8946
                     /locus_tag="VBC94_000126"
     CDS             8476..8946
                     /locus_tag="VBC94_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEO6978435.1"
                     /translation="MAKFGFLSVLEEELDKHLQYDFAMDWDKKNHTVEVTFILEAQNS
                     SAIETVDDQGEMSSEDIVFEDYVLFYNPVKSRFDAEDYLVTIPYEPKKGLSREFLAYF
                     AETLNEVATEGLSDLMDFLTDDSIEEFGLSWDTDAFENGRAELKETEFYPYPRY"
     gene            9082..9540
                     /locus_tag="VBC94_000127"
     CDS             9082..9540
                     /locus_tag="VBC94_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6978436.1"
                     /translation="MDIFDELFDFSGAKIALFCEGKILTSLRDDFPDLPYAGFWDLPG
                     DGREGDETPLECLFREVSEELNLTLTRNHIDWVKTYRGMLNPDKLSVFMVGHISQKEY
                     DSIVLGDEGQDYKLMSIDEFLSHKKVIPQLQERLRDYLEVEDDYFTKGGS"
     gene            9512..9982
                     /locus_tag="VBC94_000128"
     CDS             9512..9982
                     /locus_tag="VBC94_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6978437.1"
                     /translation="MITLQKAEASDLEKIIAIQRASFKAVYEKYHDQYDPYVEEVEQI
                     RWKLVERPDCFYHFVLVDETIVGFLRLVIKDEEKRAWLGTAAILPQYQGQGYGSAAMA
                     LLEKTYPKLTKWDLCTIAQEKLMVSFYEKCGYHSTHTEPEQEGMDMVYMTKKIE"
     gene            complement(10021..10737)
                     /locus_tag="VBC94_000129"
     CDS             complement(10021..10737)
                     /locus_tag="VBC94_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6978438.1"
                     /translation="MKTVKEVSILSGVSVRTLHHYDKIGLFPPTALSEAGYRLYDDEA
                     LIRLQEILLFRELEFPLKDIKYLLEQAKEERQDLLAQQIKLLEWKRSHLEQVITHAKR
                     LQEKGYAYMNFDVYNKTELEQLQAEAKEKWGQTAAYQEFAQKHASDDFAQISQEMAKI
                     MVQFGQLKTQNVSDESVQMCVKRLQDYISQHFYTCTNEILAGLGQMYQSDDRFSQSID
                     KAGGAGTSEFVSQAIAYYCQ"
     gene            10861..11319
                     /locus_tag="VBC94_000130"
     CDS             10861..11319
                     /locus_tag="VBC94_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HEO6978439.1"
                     /translation="MEIVELLREWYSSFEVKEEKWNEKYEGILIRIEGDGNYKRCRLA
                     HKTASKMGYFTVFWQKDGNGKNIPFYEQDEVEDLIIVIKDGRRKGLFIIPKEVAVSKG
                     ILSSANSQGKMAMRFYSPWCSDLNRTALVTQRWQLNYFIDLSKNNEGVTT"
     gene            11316..12269
                     /locus_tag="VBC94_000131"
     CDS             11316..12269
                     /locus_tag="VBC94_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEO6978440.1"
                     /translation="MNTWNELTVHVNREAEEAVSNLLIETGSQGVAISDSADYLGQED
                     RFGELYPEVEQSDMIAITAYYPDTLDIEAVKADLADRLANFEGFGLATGSVNLDSQEL
                     VEEDWADNWKKYYEPARITHDLTIVPSWTDYEAKAGEKIIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVIDVGTGSGVLSIASSLLGAKDIYAYDLDDVAVRVAQENIDMNP
                     GTDNIHVAAGDLLKGVQQEADVIIANILADILIHLTDDAYRLVKDEGYLIMSGIISEK
                     WDMVRESAEKAGFFLETHMVQGEWNACVFKKTDDISGVIGG"
     gene            12269..13009
                     /locus_tag="VBC94_000132"
     CDS             12269..13009
                     /locus_tag="VBC94_000132"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEO6978441.1"
                     /translation="MQQYFVNGKADDFVTIEDKDTIKHMFNVMRLTEDDQVVLVFDDA
                     IKRLAKVVDSSAHRFQILEELDSNVEMPVQVTIASGFPKGDKLDFVTQKATELGAAAI
                     WGFPADWSVVKWDGKKLAKKEDKLAKIALGAAEQSKRNRLPQVRLFEKKADFQAELAG
                     FDKIFIAYEESAKEGEMSALAQNLQTVKAGDKLLFIFGPEGGISPKEIAAFEEVGAIK
                     VGLGPRIMRTETAPLYALSVISYSAELL"
     gene            13006..13592
                     /locus_tag="VBC94_000133"
                     /pseudo
     CDS             13006..13592
                     /locus_tag="VBC94_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
     gene            13793..14674
                     /locus_tag="VBC94_000134"
     CDS             13793..14674
                     /locus_tag="VBC94_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEO6978442.1"
                     /translation="MKKHKMLSLLAVSGLMGIGILAGCSNDSSSSSKGTINIVSREEG
                     SGTRGAFIELFGIESKNKKGEKVDHTSDAATVTNSTSVMLTTVSKDPSAIGYSSLGSL
                     NSSVKVLKIDGKNATVKDIKSGSYKISRPFNIVTKEGKEKEATKDFIDYILSKDGQAV
                     VEKNGYIPLDNAKAYQAKVSSGKVVIAGSSSVTPVMEKIKEAYHKVNTKVDVEIQQSD
                     SSTGITSAIDGSADIGMASRELDKTESSKGVKATVIATDGIAVVVNKKNKVNDLSTKQ
                     VKDIFTGKTTSWSDLSK"
     gene            14689..15534
                     /gene="pstC"
                     /locus_tag="VBC94_000135"
     CDS             14689..15534
                     /gene="pstC"
                     /locus_tag="VBC94_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO6978443.1"
                     /translation="MKQTKEGLMQAVFFITACVSVISAILICLFLFSSGLPAITKIGW
                     GNFIFGKVWHPSNNIFGIFPMIVGSLYVTAGALLLGGPIGILTAVFMAYFCPENIYRP
                     LKSAINLMAGIPSVVYGFFGLVVIVPMIRQYIGGFGMGVLAASILLGIMILPTIVSIS
                     ESSLRAVPESYYEGGIALGASHERSVFFAVLPAAKRGILASVVLGIGRAIGETMAVIM
                     VAGNQAVLPQSLTSGVRTLTTNIVMEMGYSSGLHRQALIGTAVVLFIFILMINISFSA
                     LQKEE"
     gene            15536..16414
                     /gene="pstA"
                     /locus_tag="VBC94_000136"
     CDS             15536..16414
                     /gene="pstA"
                     /locus_tag="VBC94_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO6978444.1"
                     /translation="MENINQRTSKQYLASLKKDPLSFFLFAIVYLGAILSFATIAFVV
                     IYILVKGLPHVNTGLFAWTYNTQNVSLLPAFINTIFIIALTLLFAVPLGIGGSIYLTE
                     YARRDNPYLKIIRVATETLAGIPSIIYGLFGALFFVKYTHLGLSLISGSLTLSIMILP
                     LIMRTTEEALLSVPDSYREGAFALGAGKLRTIFKIVLPSAMSGIFAGIILAIGRIIGE
                     SAALIFTAGTVAKVVHSVFSSSRTLAVHMYAISGEGLYVDQTYATAVILLLLVIIVNF
                     VSGLVAKRLGSESNSE"
     gene            16407..17156
                     /gene="pstB"
                     /locus_tag="VBC94_000137"
     CDS             16407..17156
                     /gene="pstB"
                     /locus_tag="VBC94_000137"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6978445.1"
                     /translation="MSKLVINNLDLYYGEFHALKDVNLDIEEKEITAFIGPSGCGKST
                     LLKSINRMNDLVKNCKITGDITLEGEDVYRQLDINQLRKKVGMVFQKPNPFPMSIYDN
                     VAFGPRTHGIHSKAELDDIVERSLKQAALWDEVKDRLHKSALGMSGGQQQRLCIARAL
                     AIEPDVLLMDEPTSALDPISTAKIEELVIQLKKNYTIVIVTHNMQQAVRISDKTAFFL
                     MGEVVEYNKTSQLFSLPQDERTENYITGRFG"
     gene            17153..17809
                     /gene="phoU"
                     /locus_tag="VBC94_000138"
     CDS             17153..17809
                     /gene="phoU"
                     /locus_tag="VBC94_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO6978446.1"
                     /translation="MIRSRFASQLNDLNKEIIFMGALCEDIIGKSLGALTNSNDVYLD
                     DISETYHKIEQMERDIEERCLKLLLRQQPVAKDLRRISSALKMVYDMKRIGAQAYEIA
                     EIVSLGHIIQGAGSERDQLNSMSNNVISMLTKSIDAFIYDNEEQAHQVIEQDRTVNQE
                     FDTIKKQLVLYFSVQDVDGEYPIDVLMIAKYLERIGDHTVNIAKWVLFSITGNLDGEA
                     "
     gene            17809..18486
                     /locus_tag="VBC94_000139"
     CDS             17809..18486
                     /locus_tag="VBC94_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6978447.1"
                     /translation="MIYCVEDDADIREMMLYTLQMAGFTAQGFSSSELFWEAVQEKVP
                     DLILLDIMLPGDDGLTILERLRRKHQTEMIPVIMTTAKGSEYDKVKGLDLGADDYLVK
                     PFGMMEMISRIKAVLRRSRQVDSKAHIIIGNLEIDPTNYWVKRGTEKIHLTLKEFELL
                     VLFFRNPNRVFTRQELLDKVWGEQFLGETRTVDVHIGTLRTKLGEDGYLIATVRGVGY
                     RLEERHD"
     gene            18479..20134
                     /locus_tag="VBC94_000140"
     CDS             18479..20134
                     /locus_tag="VBC94_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO6978448.1"
                     /translation="MTKKIFRTTLSASLGIVLVTILMIMGFLYNYFNRIQREQLRTQT
                     ALASQGISFEGKDYFENLKTSNVRITWVDNKGQVLYDTQSDAKHMKNHANRQEIKEAI
                     KSGYGESTRWSATLTEKSIYAAQRLNNGTIVRLSVAQQTIFYLLLGMMSPLAIIILLA
                     IILSVLIARYIAKKVSEPLNNIDLDHPLSNDSYEEITPLLRRLDSHQAKIQHQKLLLQ
                     KRQKEFDTIISKIKEGMILLDDQARIVSINAEALKLFQINDDWHGRFMMEVSRDLTLK
                     DLIDQGLKGKKKEANIDIENNHYRVLVRPTTDNNRVTGLVVLLFDVTDQLQMEQLQRE
                     FTANVSHELKTPLHVISGYSELLANQMVPNEEVPQFAAKIHKESERLVKLVEDIINLS
                     HLDEQEKLPQETVNLYDLTQKVLEGLQAKADKKHIQINFNGEEAILRGNPVLLNSLVY
                     NLCDNAITYNHEKGQVNVTLKNSPDTITLEVSDTGLGIAEKDKKRIFERFYRVDKSRS
                     KIVGGTGLGLSIVKSALDFHNGSIKVDSHLGQGTTMTVLLHKQ"
     gene            20289..22472
                     /locus_tag="VBC94_000141"
     CDS             20289..22472
                     /locus_tag="VBC94_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO6978449.1"
                     /translation="MKNNVKQLFSFEFWQKFGKALMVVIAVMPAAGLMVSIGNSIRLL
                     DPSNVLLGRIANVIAQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLSFILINLI
                     TGNFFGVKTDMLANSKATVQTVFGATIRVSDYFVNVLGQPALNMGVFVGIISGFVGAT
                     AFNKYYNYRKLPDALTFFNGKRFVPFVVIYRSVIVALILSVFWPVVQSGINGFGKWIA
                     SSQDSAPILAPFVYGTLERLLLPFGLHHMLTIPMNYTQLGGTYTVLTGATKGAQVLGQ
                     DPLWLAWVGDLINLKGSNSSQYHHLLTSVTPARFKVGQMIGASGILMGLSYAMYRNVD
                     KDKKLKYKSMFISAAAATFLTGVTEPIEYMFMFAAMPLYLVYAVVQGCAFAMADIVNL
                     RVHSFGNIEFLTRVPMGIKAGLGGDIFNFVWVTLLFAVLMYFIANFMIKKFNLATAGR
                     NGNYDNEEVDNAPSTASGSADANSQVVQVINLLGGRDNIEDVDACMTRLRVTVKDGNS
                     VGSEAAWKKAGAMGLVLKGNGVQAIYGPKADVLKSDIQDLLDSGTVIPIVDLETGQPV
                     AATQVTTYKGITEEIVSVANGQVEALDVVKDPVFSQKMMGDGFAVEPTDGNIYVPVSG
                     TVTSVFPTKHAFGLLTESGLEVLVHIGLDTVALDGQPFEVKISSGQKVVAGDLAVVAD
                     LEAIKAAGKETSVIIVFTNVSDIKTVKLEKSGPQIAKTVVAKVEL"
     gene            22511..23341
                     /locus_tag="VBC94_000142"
     CDS             22511..23341
                     /locus_tag="VBC94_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEO6978450.1"
                     /translation="MKGYSMAKCLTLNTHSWMEVNALKKLFDLAEHIFREKYDIICLQ
                     EVNQSISSPLATSSPNYHPIEGTPALHQDNFALQLVHYLNLQGLHYHWTWAYNHIGYS
                     KYHEGVAILSLKPLKPDDILVSAVDDETDYHTRRALVAETTLNDKVVTVVSLHLSWFE
                     KGFAEEWKRLETTLLEVETPLLLMGDFNNPTGNQGYELVLNSPLALKDSHQIANHVFG
                     DHTIMADIDGWEGNKKALKVDHIFTSEDLSISSSQVVFEGGEAPVVSDHYGLEITLNL
                     "
     gene            23557..24309
                     /locus_tag="VBC94_000143"
                     /pseudo
     CDS             23557..24309
                     /locus_tag="VBC94_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645527.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            24306..25640
                     /locus_tag="VBC94_000144"
     CDS             24306..25640
                     /locus_tag="VBC94_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO6978451.1"
                     /translation="MNSTLSILEIALTIILTIFLFSKISGRQLTLIEIGICIALRLGL
                     VILFIAANTIINLSFLKYWLLPIYMLSLAFLLLRPISKTLVVFYGLFPIVLRNLCSRS
                     LAFFVFPLFNIDFHTTNQGFWGYFVDVLSTLCVLLFLRWMQYDFKTLRSSEVSPIDKR
                     ILQFTNGCMVSYYLLMQFSTYLEYELAINTVIYRQFLLVVYWIIFTGAIASLDRRLRN
                     SLQERLAVQRELQLANMIDYSHHIEGLYKEVRSFRHDYSNMLTTLKLGIDNEDISIVK
                     SVYQSIFKESNTHFRHPKYNIDRLVNVDNPALKSLLAAKLSQAIDNHIMVSIEVPDSI
                     KPQGMELIDFITIVSVLWDNAIEASLETDDPKIAMSYFTLKHKQIFIIENSTKEKAID
                     TSTIFNYEISSKGRNRGIGLYNVRGVINRYPNISIATSSQDFSFCQTFEIIL"
     gene            complement(25729..25893)
                     /locus_tag="VBC94_000145"
                     /pseudo
     CDS             complement(25729..25893)
                     /locus_tag="VBC94_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632309.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            26182..26355
                     /locus_tag="VBC94_000146"
     CDS             26182..26355
                     /locus_tag="VBC94_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO6978452.1"
                     /translation="MKHYHMLKVALVIDGYTVRTGKHTYTSAWHLSENTAAANARNRM
                     GLYPGSVCRVYLV"
     gene            26424..27272
                     /locus_tag="VBC94_000147"
     CDS             26424..27272
                     /locus_tag="VBC94_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6978453.1"
                     /translation="MLLQLDNITKSYGKKIVLNQISYQFTPGLYGLLGANGTGKTTLL
                     NLMSHFTFADSGNIYWNGQEQSEEFYRHIGFLPQHFRYYDQFTGIAFLNYIATLKGVD
                     KKKAKPEIPRLLELVGLGDVGKKKISSYSGGMKQRLGIAQALINDPEILILDEPTVGL
                     DPKERVKFRHILSQLSTNKIIILSTHIVSDVEAVAKEIIVLKNGKFIEQGNTAQLLKT
                     IEGKVWEITTEPDLSQIPNIAIVNEKVISDSLVFRVVSDICPSDSAQLVVPTLEDFYI
                     YHFRGE"
     gene            27278..28432
                     /locus_tag="VBC94_000148"
     CDS             27278..28432
                     /locus_tag="VBC94_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEO6978454.1"
                     /translation="MISIFPFELKKLLQRKAALGAFIIFLLAIFGLFYQHFFVGQISG
                     YSADKIHGREAVAINRRIAEKYSGDLSEERVTNIIKNYVNPPAGRPPIFDVFSYYVAD
                     TFDRDFLIYYNQLKDDKTIDFNKINIKDQKQLGSALPLKQLKLGNFAPWNQLFEVSNS
                     TYILIVLFTLFLSAPLFSGDRAKKINPILLTTRYGRGQLTWAKLASTMTIGISTFILA
                     YALILTVFADYFGFSGWETSVQLNLYWTSPLANIMGFPVPMTISTAFVHIIVLQFVGL
                     LFIASLNFFILSITNSPLTSFAVSTLAFFAPSFLMAMFNKGVINKLLTIFSVSTTDTS
                     ALLLKLSRNGSHGFFFDSFSANEICLISLRMLIAVLLIAYTYRLMRRRSL"
     gene            28661..29695
                     /locus_tag="VBC94_000149"
     CDS             28661..29695
                     /locus_tag="VBC94_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO6978455.1"
                     /translation="MDNKQHNTSMISLLQYLFSILVILVHSGRLFSQDVIHFTFKSFL
                     GRMAVPYFLICTAFFLRGRIQQGLCNHSYFRKLIKKYSMWTIIYLPYGYFFFESLNIA
                     KIYLLPGFIVAFLYLGMSHTLWYIPAVILGWFIIQGLLKYVGTRGTFITVVVLYCIGA
                     VETYSVFVQSTKFYPLMSTYMSIFQTTRNGLFYTPVYLLAGYLLYDYFNTDLFTKSRG
                     LKYILFLLLLALENVLIYFNQGLDKNFFLLAPLCAVFLFNWSIRTSLFKGYRLSPLKQ
                     LSVYYFFLHPLFVGIVSYCLKSTSLVAHHQGKVIFVVTLALTHACALLIIKVVNKIPY
                     QKIIPLSLSK"
     gene            29942..30367
                     /locus_tag="VBC94_000150"
     CDS             29942..30367
                     /locus_tag="VBC94_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEO6978456.1"
                     /translation="MKRKFLIGSHGKLASGLQSSIDILTGKGQEIQTIDAYIDDSDYT
                     KSIVEFIDEIAPDEQGLIFTDLLGGSVNQKMATAVMNSGKNNVFLITNSNLATLLSLL
                     FLKPEEELTKEEIVTVINESQVQLVDLSFKACSEDDFFD"
     gene            30385..30888
                     /locus_tag="VBC94_000151"
     CDS             30385..30888
                     /locus_tag="VBC94_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6978457.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEESIALFKDPRATDKRIFVIVNSVKDACTIAKNITDLEAVN
                     VANVGRFDKSDPATKVKLTSSLLLNPEELEAAKELASLPDLDVFNQVLPSNTKVNLSQ
                     LVKQSEV"
     gene            30892..31701
                     /locus_tag="VBC94_000152"
     CDS             30892..31701
                     /locus_tag="VBC94_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO6978458.1"
                     /translation="MIMPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGMLLGDI
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISNIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNSFVHHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDIELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            31698..32525
                     /locus_tag="VBC94_000153"
     CDS             31698..32525
                     /locus_tag="VBC94_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6978459.1"
                     /translation="MKSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASTGSAMGAVVFLLLYNIPAFLIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQSYLDQLFVGVVPLLVTILAFWLLRKKVNINWIMFGIMVLGIVLGLLGIC"
     gene            32663..34312
                     /locus_tag="VBC94_000154"
     CDS             32663..34312
                     /locus_tag="VBC94_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6978460.1"
                     /translation="MRGYRMEERFKKRLQDDISKHFSRQSLILSLLLIALFVLFSLAP
                     QQIGLYKDVNSVSYSYKQLIQKHDTLLDDLGKNSLKPFVSGHLGSADLSKQYYHLRNH
                     LQSQTELLVFSPNQELLFASNSHLGNFFSKSIYISEVLDKAKINQRLLKIIVDSEGGH
                     YLALIKPIIVNKKVSGYAFLLMNGKDFLLPTKAINSDLIIADQLNNSFTFTNRDFISS
                     SLDKVDSQFLTRYFSFHDHRAFVVRKVALQDNILLYMYRPLIPVTLVVLFSLVSSVII
                     FVILRRKSRVLADRIAVKNSGAINQMVLDMDAISRQEKSSIELDSQDEFQYLSVQINQ
                     MVSRLKDLHEKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSQLTERIV
                     IQLTKLLRYSLSGSTEAAVLKDDLAIIESYLLINQVRFEELTYTISVSPELEHMRVPK
                     LFLLPLIENAIKYGLKERHDVAINIDIWQDSDGIWFTVSNNGSGISLARQKAIRTMLR
                     SNHSHHGLINSYRRLQYQFSTVLLEFTKTDDAFRVSYIVKE"
     gene            34316..35089
                     /locus_tag="VBC94_000155"
     CDS             34316..35089
                     /locus_tag="VBC94_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO6978461.1"
                     /translation="MYRLLIVEDEHLIRKWLRYAIDYQSLNILVVGEAKDGKEGAQLI
                     QEEQPDIVLSDINMPIMTAFDMFEATKGQSYAKIILSGYADFPNAQSAIHYGVLEFLT
                     KPLEKQALIDCLKTIMARIEEHKEKHLQEHTELYLPLPQANDQVPEVIKDMLAWIHSH
                     FHGKIVISQLAHDLGYSESYLYTVTKKHLHITLSDYINQYRINQAIQLMFREPDLMVY
                     QIAEAVGIYDYRYFDRVFKKYLGQTVKAFKEEHIFKQMD"
     gene            35101..36138
                     /locus_tag="VBC94_000156"
     CDS             35101..36138
                     /locus_tag="VBC94_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO6978462.1"
                     /translation="MKEKQSKRLIYILLIVPIIFISVFTYSISQPSKLLPPKELVILS
                     PNSQAILTGTIPAFEEKYGIKVKLIQGGTGQLIDRLSKEGKQLKADIFFGGNYTQFES
                     HKALFESYVSKNVHTVIPDYIHPSDTATPYTINGSVLIVNNELAKGLTIKSYEDLLQS
                     SLKGKIAFADPNTSSSAFSQLTNILLAKGGYTNPKAWNYVKKLQHNINAIKSSSSSEV
                     YQSVAEGKMIVGLTYEDPSVNLQKSGANVSIVYPTEGTVFVPSSVAIIKNAPSMKEAK
                     LFINFMLSLDVQNAFGQSTSNRPIRKDAQTSNGMKALKDIATLKEDYRYVTKHKSQIL
                     KTYNRIRRNAD"
     gene            complement(36360..36857)
                     /locus_tag="VBC94_000157"
     CDS             complement(36360..36857)
                     /locus_tag="VBC94_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO6978463.1"
                     /translation="MVATLIIIRGNSASGKSTIAKQLQAELGEDTLLLSQDYLRREML
                     GTKDGENTTTIPLLINLLNYGYHNCSYIILEGILRSDWYTPVWKHILKHNPNNTYAYY
                     YDLSFQETVKRHSTRLKSLEFGEDSLARWWLEKDFLKEIPEKILTKSMSLEDVKKLIM
                     TDVLP"
     gene            complement(36857..37894)
                     /locus_tag="VBC94_000158"
     CDS             complement(36857..37894)
                     /locus_tag="VBC94_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEO6978464.1"
                     /translation="METTLNYIKTLTSIPSPTGFTQTIMTYIIKELEAFGYSPIRTNK
                     GGVMVSLKGKNNTKHRMITAHLDTLGAMVRAIKPDGRLKIDLIGGYTYNAIEGENCTI
                     HLSKNGKEISGTALIHQTSVHVYKDAGTAERNQTNMEIRLDEKVTTADETRALGIQVG
                     DFISFDPRTIITGSGFIKSRYLDDKVSAGILMELLSVYKKEDIQLPYTTHFYFSAFEE
                     LGHGANSSIPNETVEYLAVDMGAMGDDQETDEYTVSICVKDASGSYHYELRQHLVSLA
                     ENNNIPYKLDIYPYYGSDASAAMRAGAEVKHALLGAGIESSHSYERTHIDSIQATELL
                     VDAYLKSNMVN"
     gene            complement(37905..38360)
                     /gene="nrdI"
                     /locus_tag="VBC94_000159"
     CDS             complement(37905..38360)
                     /gene="nrdI"
                     /locus_tag="VBC94_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO6978465.1"
                     /translation="MPKLTLVYISLSGNTQSFVKRLSEQLSTWHNCQVSTINIKEQNH
                     QTFPVDQPFVALLPTYLEGGNGIDNGDQEILTIPLGDFIAAHNNVKNCIGIVGSGNRN
                     FNNQYCLTAKQYSERFGFPMLGDFELRGTSSDVERIANIIVETLSNFKA"
     gene            complement(38517..40919)
                     /locus_tag="VBC94_000160"
     CDS             complement(38517..40919)
                     /locus_tag="VBC94_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HEO6978466.1"
                     /translation="MSKHYFSKSIFALTVLTATTTSGLAVQAEDIVTTPSSTATKVES
                     TTPTSTIAEEKSNVTSTPTAITDASTATSTNTTANNQNPQPVATETTTGDLKPIEGEK
                     VDLRIMSTTDLHTNLVNYDYYQDKESQKIGLAKTAVLIEEAKKENPNTVLVDNGDVIQ
                     GTPLGTYKAIVKPVAENEEHPMYQAMNALGYDASTLGNHEFNYGLDYLKKIIATANLP
                     ILNANVLDFKTHQPVFKTYDIITKTFKDSTGRAVALNIGITGIVPPQILNWDKANLEG
                     KVIVKDSVKAIEEIVPTMRAKGADVILVLSHSGIGDDRYEEGEENVGYQIASIKGVDA
                     VVTGHSHAEFPSGNGTGFYEKYTGVDGINGKINGTPVTMAGKYGDHLGIIDLGLSYTN
                     GKWQVSESSAKIRKIDMNSTTADERIIALAKEAHDGTINYVRQQVGTTTAPITSYFAL
                     VKDDPSVQIVNNAQRWYVANELKGTPEANLPLLSAAAPFKAGTRGDATAYTDIPAGPV
                     AIKNVADLYLYDNVTALLKVTGADLREWLEMSAGQFNQIDPNNKAPQNIINTEYRTYN
                     FDVIDGLTYKFDITQPNKYNKDGKVVNSQASRVRDLMYNGKPVADKQEFMIVTNNYRA
                     SGTFPGAKNATMNRLLNLENRQTIINYIISEKTINPTADNNWGFTESIKGLDLRFQTA
                     DKAKNLVTNSKDIQYIASSTKDEGFGDYRFVYTEQEKVDLTEPSHQEKLSQKEKQKIH
                     HMVATAYTKAKASPNIAGTIYTQQKEKTLPKTGQEKQSYLAFFGVAILGLVGNLLKKK
                     QI"
     gene            41179..43395
                     /locus_tag="VBC94_000161"
     CDS             41179..43395
                     /locus_tag="VBC94_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEO6978467.1"
                     /translation="MVKEINLTGEEVVAITSQYMSETDVAFVKFALNYATAAHYYQAR
                     KSGEPYIIHPIQVAGILADLHLDAVTVACGFLHDVVEDTEITLDEIETDFGKDVRDII
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLSFRYLNETEFYKISHMMSEKRREREELV
                     DIIVDKIRSYTEEQGLYGDIYGRPKHIYSIYRKMRDKKKRFDQIYDLIAIRCIMETAS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKEMHQV
                     AEFGVAAHWAYKKGITSKVNQAEQSVGMGWIQELVELQDESKDAKDFVDSVKEDIFTE
                     RIYVFTPNGAVQELPRESGPIDFAYAIHTQVGEKATGAKVNGRMVPLTAKLKTGDVVE
                     IITNPNSFGPSRDWIKIVKTNKARNKIRQFFKNQDKETSINKGRELLVDYFQEQGYVP
                     NKYLDKKHIEEILPRVSVKSEEALYAAVGFGDLSPISIFNKLTEKERREEERAKAKAE
                     ADELINGGEIKTDKRDVLKVKSENGVIIQGASGLLMRIAKCCNPVPGDLIEGYITKGR
                     GVAIHRSDCQNLKSQENYEQRLIDVEWDDDGSKKEYMAEIDIYGLNRSGLLNDVLQTL
                     SNATKLVSTVNAQPTKDMKFANIHVSFGISNLAQLTTVVDKIKIIPDVYSVKRTNG"
     gene            43405..43848
                     /locus_tag="VBC94_000162"
     CDS             43405..43848
                     /locus_tag="VBC94_000162"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEO6978468.1"
                     /translation="MKIIIQRVSQASVSIEDDVVGSIEKGLVLLVGIAPEDTTEDIAY
                     AVRKITSMRIFSDDEGKMNLSIQDIKGSVLSISQFTLFADTKKGNRPAFTGAADPVKA
                     NQFYDIFNQELANHVSVETGQFGADMQVSLINDGPVTIVLDTKNK"
     gene            44103..45026
                     /locus_tag="VBC94_000163"
     CDS             44103..45026
                     /locus_tag="VBC94_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEO6978469.1"
                     /translation="MKRIRKSLIFVLGIVTLICLCACTKQSQQKNGLSVVTSFYPVYS
                     ITKAVSGDLNDIKMIRSQSGIHGFEPSSSDVAAIYDADLFLYHSHTLEAWARRLEPSL
                     HHSKVSVIEASKGMTLDKVHGLEDVEAEKGVDESTLYDPHTWNDPVKVSEEAQLIATQ
                     LAKKDPKNAKVYQKNADQFSDKAMAIAEKYKPKFKAAKSKYFVTSHTAFSYLAKRYGL
                     TQLGIAGVSTEQEPSAKKLAEIQEFVKTYKVKTIFVEEGVSPKLAQAVASATRVKIAS
                     LSPLEAVPKNNKDYLENLETNLKVLVKSLNQ"
     gene            45053..47635
                     /locus_tag="VBC94_000164"
     CDS             45053..47635
                     /locus_tag="VBC94_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6978470.1"
                     /translation="MNRKKTVIISALSVALFGTGVGAYQLGSYNAQKSDNSVSYVKTD
                     KSDSKAQATAANKTPDQISKEEGISAEQIVVKITDDGYVTSHGDHYHYYNGKVPYDAI
                     ISEELIMKDPSYVFNKADVINEVKDGYIIKVNGKYYLYLKEGSKRTNVRTKEQIQKQR
                     EEWSKGGSKGESGKHSSAKTQALSASVREAKASGRYTTDDGYVFSPTDVIDDMGDAFL
                     VPHGDHFHYIPKADLSPSELSAAQAYWNRKTGRSGNSSKPSNSSSYIHASAPSGNVST
                     GRHANAPISIPRVTHANHWSKPAGNHATAPKHHAPTTKPIKKDSALDKMLKRLYAQPL
                     SARHVESDGLVYDPAQVNAFTAIGVSIPHGNHFHFIHYKDMSPLELEATRMVAEHRGH
                     HIDALGKKDSTEKPKHISHEPNKEPHTEEEHHAVTPKDQRKGKPNSQIVYSAQEIEEA
                     KKAGKYTTSDGYIFDAKDIKKDTGTGYVIPHMTHEHWVPKKDLSESELKAAQEFLSGK
                     SEANQDKPKTGKTAQEIYEAIEPKAIVKPEDLLFGIAQATDYKNGTFVIPHKDHYHYV
                     ELKWFDEEKDLLADSDKTYSLEDYLATAKYYMMHPEKRPKVEGWGKDAEIYKEKDSNK
                     ADKPSPAPTDNKSTSNSSDKNLSAAEVFKQAKPEKIVPLDKIAAHMAYAVGFEDDQLI
                     VPHHDHYHNVPMAWFDKGGLWKAPEGYTLQQLFSTIKYYMEHPNELPKEKGWGHDSDH
                     NKGSNKDNKAKNYAPDEEPEDSGKVTHNYGFYDVNKGSDEEEPEKQEDESELDEYELG
                     MAQNAKKYGMDRQSFEKQLIQLSNKYSVSFESFNYINGNQVQVTKKDGSKVLVDIKTL
                     AEVK"
     gene            complement(47916..48692)
                     /locus_tag="VBC94_000165"
     CDS             complement(47916..48692)
                     /locus_tag="VBC94_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6978471.1"
                     /translation="MLKRERLQKIIEKVNINGIVTVNEIMEELDVSDMTVRRDLDELD
                     KAGLLIRIHGGAQKVNAAPIPQNYEKSNTEKYDIQTNEKLEIAQFAKQFINDGETIFI
                     GPGTTLEKLATQLLDFKIRVVTNSLPVFNILNQSSTLDLILVGGEYREITGAFVGSVT
                     INSIKSLNFSKAFVSSNGVFEKSIATYDEGEGEIQRIALNNSFEKFLLVDSQKFGKYD
                     FYTFYQLDDIDFVLTDHNIDNVVKEQYSSFTKILTNNNYM"
BASE COUNT        15917 a         7687 c         9638 g        15603 t
ORIGIN      
        1 tataaaagtg agtatccaag taaaaaatta aagttactat aatcatttta aagtgaaaac
       61 gaaaactcta atgttacact ttggaaaata taaaatataa agtcctgcaa ggagaaacat
      121 ggaagcaagt gatgtaatca aaaagataga aaataattat ccaattgatg agaatgtttt
      181 atctttttga ttacatcata taggagattg gaacccgaga gttcgagatg aaatagtata
      241 tgggtcttgg gttaaattag taatagaaca taaactgatg caaagccaga aattaaatat
      301 ccttcagaga gttttaaggg ataaatttct tttacaagga ttaggagtac ccaacagtaa
      361 tactgtgtta aacagatctt ttactgctct attactagct ttattgttgg aggactcaaa
      421 taataaaagt tggatatctg aagaagatca gataaaaatt atgaatcagg cattctcctg
      481 gcttatagat gaacttgatt ttagaggctt tgacgaagag cttggatgga tacatgtctt
      541 tgctcatgga gctgatttat taacagaaat agtcaagatg aaatgttttt caaaagatga
      601 cagttcaaaa atcttaacta tcataaaaag agtgatgata actgatgaca tactaacatt
      661 aaacggttga aaatattttg aatcataatc agttgttcag aaaaatttaa aattgaatcc
      721 ttggaggtcg taaaggtatt tccatgctaa tttaagttta tgattgcatg cttagagaaa
      781 aatacaaggg taatataaag gtattagaaa aaatatggta atataaagtg aagtaggagg
      841 tagccattat gagagatgag caagaaatat ataatttggt tttaaacata gcaaatcaag
      901 ataagagaat cgaagctgta ctattgaatg gatctcgggc taacccaaat gtaccgaaag
      961 atgattttca agattatgat attgtttttg ttactaattt tatagaagat attatttctg
     1021 atactaatta tcataaaaaa ttcggtgata ttcttataat gcagaaacca aatgagttta
     1081 gaaataagac ggaatacaat tgctttgctt acttaatgca gtttcaagat ctgacgagga
     1141 ttgatttaag acttattaaa ccagaattct tggaggatta tttagatgat gctttttcaa
     1201 aagtactttt agataaaaag aataaatatc ttgattataa ttttgaaaga agttctctgt
     1261 acgaaacaaa gcaattgtca gaagatgaaa tcaacaaaat tcttaatgaa atatattggg
     1321 ttagtactta tgtagtcaag gggatagcaa gaaatgatat tatgtattca gagtttatga
     1381 tatctaatcc tattaaaaat gcttttatta aacttctaaa acaaaaaata ttaatagaaa
     1441 aagagcttga ttcattatct tttggaaagc tcgacaagga tatcttgcaa tatattacag
     1501 ataaagatca actattgaaa attttttcaa ataaatcttt aaaagacatt gagtctaatt
     1561 tgcgattttt attggatgaa acaaatcaaa tggcgaaata tatttctatt aataggaaac
     1621 ttaatctgaa ccaaggagaa taccaatcag caatgaaatt tatgaatatc tttttgtcaa
     1681 attcatatca aaatttcaac taatattatt gtgtaaaagg ataataggct gtgattgtct
     1741 aaactcaatt actattcata aagattttca agaaaaataa taaataattt tggggacaaa
     1801 tgatgggaca aatcctcttc tgtcaagcaa aaaagcctaa gaacaattgt aataaggaga
     1861 aaagatgaaa aaaaatattt attacttctt ccaatagtat taattatagt ggtagtagtt
     1921 ggcatactta atcataaaaa aatgccagat gaaggaagat attatttgac tgaaaaaaat
     1981 tataataatc atactatctc attaaataaa actgaatttt ttaccataac cgatgatcaa
     2041 gttacctata caaaaaatga ggagttagaa aaaatatctt atgatagcaa aaataatgaa
     2101 ttgcttttaa atggtaaaaa gttttggact catctcactt ctggtgagct tcagttaaca
     2161 gatcctaaga acactgatat gacattaaat tatgctagta aaaatagtcc tttattcaaa
     2221 tcatatgaaa aaggaacagc aaaatttaaa gaggaaaatt aaattatatt attaactgct
     2281 gaataagtta tggtgcaaga catacttgtg ggagtgttaa ggttcaagaa ggagtagtat
     2341 tgaatcttca attatttggg aatctgaatt accttcttca agaaaaaaga ggtaatttaa
     2401 ttaatttaaa agaagttatc tatatttgaa tggtatagaa aaaacagagg tttatttgct
     2461 tatgaaaaaa cgtatatggt atttgataat aataatcaca gtaattttag gaggactagc
     2521 catgaaaaac ttatttgcaa caacagaagc atcatcaagg aaacaggaac aagatagaat
     2581 tgtcaattac ataaaacaac atgttgagtt aacaaatggt aatcaaataa aaaaaattga
     2641 gtttatcgac tttcaaaaaa atgagatgac aggtacatgg ggaatttcta ctaaaattaa
     2701 tgaacaattt tcgattagtt tttctgaaga tagaattggt ggtaaactta gagcattagg
     2761 atatcaaccg aatgaaatag gtttttcaaa ggacatcaat agtaataatc aaaatgttaa
     2821 tgatattgaa gtgatttata tgaagaaaga atagaatgat ttcagaagaa agttacagat
     2881 atttagttga ggatgcttat cgagttgatt caaaaaaagt aaaaatacct ctgaaaagaa
     2941 gtgatattgt agggaacagt gactatgtta ttattgaacc acctatagat aatacttcaa
     3001 atggtatgca agctatggta gttgctccga taaaagaagg aatgacagct gaaccagata
     3061 ctagtgaaat agtgattgtt tatgcaggta ctaatttagg tgagcgttta gacatagcta
     3121 cagatgttga aatggttgct ggaggggata catatttact tgctgatcct aaaaccaaga
     3181 cttttaggaa atctcaggga aaatcagcac tagagtacgc agaaaaaata tcgagtaaat
     3241 atcctaattc agaaattact acaaccggtc actccttagg ggaatcagag gcactatatg
     3301 tcgctttaaa aatgggttgg atgaatgttg gttataatgg tccagatatt cataacatga
     3361 tataaaatga cgaaatagat tatatgaaat ctcatccaga ataatttaga aaaaaatcga
     3421 aaaatatgag ttgtttgagt taaaatttta actatggaga tagagtcctg tattatttga
     3481 ttttgtagta aactagttag gaatttattc ataggatatt ataaaggagt taatgatgaa
     3541 gaaaaaatat tggatattac taatattact attaacagtt ttatcaggag gcggatatat
     3601 ggtttttaaa caacatacag aaaaagaaaa aatgattgca attgcacaca gtgcagaagc
     3661 gatgcaaatt tatgagaagc atatgaaagc aaatgattcg cgagcttttt cagagcaagg
     3721 aattattcat aattataaaa ttaacgattc agaactcaat tataatccta tgggaggaat
     3781 ggaggttact gtttatgtga ataatgatac caataaatat tttcaattta ctattttaga
     3841 taatggaaat ggtaaacttg aatctgggag ctatattata agtgcagatt tagataatga
     3901 tttagaaacc aataaaaaat gaaaaaaata tattgatgaa gaaaaattac aaatcgcaat
     3961 gtcagaatat aaaacaatcc atcccaacgc cagattaaag gatcaatatg ataacacaat
     4021 tggagttgtt tcggaggttt acgacaatac ttctcattct ggagaacaga tttatgctgt
     4081 ggttgataat cgtcatattc caaaagaaga tgtaaaagag gtgacggtac tttttcgtgg
     4141 atcaacatct cctcaggaat ttattaataa tcctgcagat gtgactttgg attggttaga
     4201 aaatgacatt cctatggcaa gtaatatttg ggtaatgaaa gattttggga atcctcataa
     4261 tttcagtgct gtatcaccac agttgaaagc ttcatcagaa catttaaaag agattatgaa
     4321 aaaatatcca aatgcaaaaa taaacattgg tggacattca ttaggaggta tggatgctca
     4381 atatgctgtt gtagatatta cagataagaa agacttgaaa cggattaact ctgtacatgt
     4441 ttataatgct ccagatatat atcctacact tactaaagga cagaaaaaga cggcagattc
     4501 actgaaatct aagattgttg tttatgttga tcctaatgat tttattggca tggtggggcg
     4561 tgaaggaaaa aaaggaagtg aagactctgt aggaacagtc tattatacag aaagtccaga
     4621 tatcaactgg atagatcaac acatgaccta tggctatcgt atggaaaatg gtcagattaa
     4681 agttattgaa acgaatttac caccggaagt taggactact agaaaagcta tgggatcttt
     4741 ttataaggac aagaaaaaat ttaaaaaatc tggtaagggt ctaagttcgc atgaaaaaat
     4801 cttcttggat gctgaacaag ctgctgttat tgcaagtggt ttagtctcta ctgctgaaac
     4861 tgctttagag gatatcaaaa catctgcgaa aacagctgtt ggagaagcag aagaattgtg
     4921 gaatacgact aaatcgattc cttttggtat tacggagctt acagaagcag agttggcaga
     4981 agcttatgca gaaggtggtg tcacttatga tagcatagta actaaaactg atactcattt
     5041 tgatgagaaa gtcaaaaatg caggagattt agtaacaaca tatacgactt tgaaaagtga
     5101 tattcaatca ggtgttgaaa ccatgttatc taaggatagt gaattagctg gagattttaa
     5161 agcatgggaa aattagacgc taaagatgat aaattaagta acagtaaaaa ggatttagaa
     5221 aagttggaag atgattacca tcataaaacc atagctattt cagataaatt ctttgaactg
     5281 gaagataaac gatgtgagtt tgagacgatg cttcaggaaa cttacgaagc aacaagttat
     5341 catcttcgtc aggatgagat gattaatgaa gagtccttta tgaccatgaa tcacattata
     5401 gaagcttttc aaagtgattt tgatacagaa tatactaaag agaaaagaag attgactgcc
     5461 ttagaagaag aaactaatca aaagtactct aaaaagagac aattgcttga agaaaaaata
     5521 gatcatttaa tgtcagaaag gagagactat gggaatcctt ggtgattatg ttttatcaga
     5581 agcctctgat tatatcagtg gaaaaaaaga tatcaaggct ttaaaaaaga aacttgataa
     5641 aatgttagtg acaggtgttt atgctccaag aatcaagtct aaacgtagaa gtattgtctc
     5701 tacctatgat gcagagataa cgactactgc aacaaacgca aaagctagta taactgccat
     5761 tgcaggtcaa atagacactg caataaaggg acagtttaga actaaagttg aaactgtatt
     5821 ggataataac agtacaaagt atgatgaaat ttaaaagaca tgggctaaat aatttagctc
     5881 tttttttata ctcaaaaata agagaaatac aaaacaaaat acatatttat gcaagtaaag
     5941 agaaataaaa aaatcaccat tttatctgtc aaaaaaaatt ggtgggcaag tgtaaaaaga
     6001 gcgtcaaaaa agcctatagg tataggcttt gttttataag actgccccct acagggctcg
     6061 aacctgtgac ccatagatta agagtctact gctctaccaa ctgagctaag gaggctagga
     6121 agctgtattg gcaccgatga tccacttttt gtattgatcc cgcacaaaat aagcaggtgg
     6181 gtaactcgca cctctttaag tggcttccgc gtgttaacgg cttgcgcact agagatgact
     6241 tcgtttcccg aaatacaaag aaatagtcgg tcaacactta gatgtggatt cgtatgccac
     6301 agcaatgttt ctatgtcttt atgataccac tttttataaa attttcaagc aaaattatcg
     6361 atttttgaaa tcgattgcga tttttaaaac tggcaatcag tctgagagct tatcaatgcg
     6421 ctctttattt gaaccaagtg cacgttcgta tttaccagtt tcattggctt caaaataatt
     6481 atgtcctact agtttatctg gtaagtattg ttgtttaacc catttttcag gataggcgtg
     6541 agggtacttg taatcccttg cattgcccaa ggttttacta ccgctgtaat gtccatctct
     6601 taggtgacgt ggaataggta aatttcctga acgacgtaaa tcagctaagg ctgcatccat
     6661 agccaaatag gctgaatttg atttaggtga tagagctaaa tccacaacaa tgttagcaat
     6721 aagaatacga gcttctggaa aaccaatgcg ttgggcagct tccaatgcag tcacagtatg
     6781 tatttgtgct tcggggttag ctaagccgat atcttcataa gcaataatgg tcaaacgtct
     6841 tgctaaacta ggtaaatctc cagcttctac taaacgagcg gcatagtgca ggctggcatt
     6901 gacatcagaa cctctaattg atttttgtaa ggctgatagg acatcataat gcccatctcc
     6961 gtttttatcc attgtaatat aactacgttg cagactattt tccattgtct ctagactaat
     7021 atgacggcta ccatcttcgt taggagaagt agacatgact gctagatcca aggaattata
     7081 agcagatcgt aggtctccat ttgtggcggt aacaatgaaa tctagcgccc catcatcaat
     7141 tgtcactaaa aaaggaaatc cccgttcttt atcggaaatt gctagttgaa tggctttttt
     7201 aatatcctcg ttagaaagag gttctagctc aaaaatttgt accctacttc taatggcagg
     7261 agttactgaa aagaaaggat tttcagttgt tgctccaatc ataataatat tgccattctc
     7321 aagtaaaggt aaaagaaaat cttgttttgt cttgtcaagt ctatgaatct cgtcaagtaa
     7381 taaaactaag ccaccagaaa atttagcttc ttctgcaatt tcttgaagac gttttttact
     7441 atcaacagtg gcattaaaag tacgaaaggc atatttagta gtaccagcaa tagcagaagc
     7501 aatagaagtc ttcccaatac ctggtggtcc atataggatc atggaagata acatattagc
     7561 tgcaaccatt cgatcaataa ttttgccatt accaacgaga tgttgctgcc caatgacttc
     7621 attaatattg cgaggacgca tacgtagagc aagattgtca gccatgatta ttctccaaat
     7681 cttttgcgaa aattaaagca tcaacataat gattttcaat gaaaaaggct ttagtgattc
     7741 gagcctcttc ggtaaaaccg tatttttgat agagggaaat ggctctacta ttgttgccca
     7801 tgacgtgcat ggcaatcttg atgtaacctt gagctttagc ctcagttagc agagctttca
     7861 agagagcctt acctagcccc tgaccttggt agggttcagc gatgagaatg ccgaaagtgg
     7921 caacatgctt gccagctggg aaaggatagt agggaccaaa gtccaagacg ccagcgatct
     7981 tatcatcgat ctcagcgaca aagaaggaca tgccatttgc cagattcttc tccaagtcac
     8041 tctcagtcca cttgtggtca tagacaggag aggtaagagc agtccagcca gtattttgta
     8101 aggtcagaac tgcctcacgg tcgcagttct gcataggtct gataatcata attgatgata
     8161 caaagggacg gttcggttta cgaaaatttt cgtaaagaaa tagggaacga cgaagtgtcg
     8221 ttagacacta ggaagttcat cttttctttt agaaaattag tcccgtgttc aaatcctttc
     8281 tattttttat atagagtttt tctctcagct gactctttta acatagtatg aagtcagtca
     8341 aaaacgacta gtctttgaga ctattgtaac ataaaaataa gtaggaacta ccttttgatt
     8401 aacctgagtc tgtaacgttt ttcttctata ttcatgttat aataagaaag aataaataga
     8461 aaataaggac ataagatggc taaatttgga tttttatcgg tactagaaga agaattggat
     8521 aagcatttac agtatgactt tgccatggat tgggataaga aaaatcatac tgtagaagtc
     8581 acttttatat tagaagcaca aaacagctct gctattgaaa ccgtggacga ccagggggag
     8641 atgagtagcg aggacatcgt ttttgaggac tatgtactct tttacaaccc tgtcaagtct
     8701 cgctttgatg ctgaggacta tctggtgact atcccctatg agcctaagaa gggcttgtcg
     8761 cgtgagtttc tggcttattt tgcggagact ctcaacgagg ttgccactga agggttgagc
     8821 gacctcatgg acttcctcac cgatgacagt atcgaagagt tcggtctttc ttgggatacg
     8881 gatgcttttg aaaatggcag agcagaactg aaagagacag agttttaccc ttatccgaga
     8941 tattagactt gatggatttg ctaaaatgtg gatatacatt ggatgatata gaaacagcca
     9001 gaccagagga tatggaacgt tattatcccc ctgaacaaat cagaaaatat ggagctttag
     9061 gaatagaatt gaggttattg catggatatt tttgatgagt tatttgattt ctcaggtgca
     9121 aagatcgctc ttttctgcga gggaaagatt ttgaccagcc taagagatga ttttccagat
     9181 ttgccctatg caggcttttg ggacttacca ggcgatggtc gtgagggcga tgaaacacct
     9241 ttggaatgtc tttttcgaga agttagtgaa gaattaaatc tgaccttaac tcgaaaccat
     9301 attgattggg tcaagactta taggggtatg ctgaaccctg ataaattatc ggtttttatg
     9361 gttggacata tcagtcagaa agagtacgat agcattgttc taggcgatga aggacaagac
     9421 tacaagctca tgtccattga cgaattttta agccataaaa aggtcattcc tcagttgcag
     9481 gagaggttga gagattattt ggaggtggaa gatgattact ttacaaaagg cggaagctag
     9541 tgatttagag aagattattg ccattcagag agctagcttt aaggcagtct atgagaaata
     9601 ccacgatcaa tacgatccct atgtggaaga agttgagcag attcgatgga aactagtgga
     9661 gcgaccagat tgtttctatc attttgtcct tgttgatgag acaattgttg gttttcttag
     9721 attggttatc aaggacgagg agaagcgtgc ttggcttggt acggcggcta ttcttcccca
     9781 atatcaagga cagggctacg gctcagcagc gatggcttta ttagagaaaa cctatcctaa
     9841 gcttacaaag tgggatttat gtacgattgc gcaagaaaaa ctaatggtca gtttttatga
     9901 aaaatgtggt tatcattcaa cccataccga acctgagcaa gaaggaatgg atatggtcta
     9961 tatgacaaag aagatagaat aaaaaaagaa aactagatga ttgtctagtt ttctgatagt
    10021 ttattggcaa tagtaagcaa tagcttggct gacaaattca cttgttcctg ctccaccagc
    10081 tttgtcaatt gattgactaa accggtcatc tgattgatac atttgtccta gtccagcaag
    10141 gatttcattg gtacaggtgt aaaaatgctg agagatatag tcttgtagcc gtttcacaca
    10201 catttgaaca gattcatcag atacattttg tgtctttaat tgcccaaatt gtaccattat
    10261 ttttgccatt tcttggctaa tttgtgcaaa gtcatctgaa gcatgttttt gagcaaattc
    10321 ttggtaggct gctgtttgtc cccatttttc ttttgcttca gcttgtaatt gttctaattc
    10381 tgttttatta taaacatcga aattcatata agcatatcct ttttcttgta atcgtttcgc
    10441 atgggtgata acctgctcaa gatgcgatct tttccattca agcaatttga tttgttgggc
    10501 taataagtct tgcctttcct cttttgcttg ctcaagcagg tatttgatat ctttgagagg
    10561 gaattccaac tcacgaaata gcaaaatttc ttgcaggcga atcaatgctt catcgtcata
    10621 aagtcgatag cctgcttcgg atagggcggt aggtgggaat aaaccgattt tatcatagtg
    10681 atgtaaggtg cgcacactta caccgcttaa gatactgact tctttaactg ttttcatggt
    10741 aataacctcc tctcacttac atagtataaa ggataaccta aggtcagggt caagcaaaaa
    10801 ataaattatt ttgaaaagta tggttaatca tattgatttg aagataaagg agggatagtt
    10861 atagaaatag ttgagctact aagggaatgg tattcttcct ttgaagttaa agaagaaaag
    10921 tggaatgaaa aatatgaagg tatcctcata agaattgaag gcgatggaaa ctataaaagg
    10981 tgtcgtttag ctcataagac agctagcaag atggggtatt ttacagtatt ttggcagaaa
    11041 gatggaaatg gtaaaaatat tcctttttat gaacaagatg aagtggaaga tttgattatt
    11101 gtcattaaag atggtaggag gaaaggtttg ttcatcattc ccaaagaggt tgcagtgagt
    11161 aagggtattc tttcatctgc taactctcaa ggaaagatgg ctatgcgatt ttactctcct
    11221 tggtgtagcg atttaaatag aacagctttg gtgacacagc gatggcagtt gaactatttt
    11281 attgatttaa gtaaaaataa tgaaggagtt actacatgaa cacatggaat gaactaacgg
    11341 ttcacgtgaa tcgtgaggca gaggaagctg tctcgaattt attgattgaa actggcagtc
    11401 agggggttgc cattagcgac tcggctgatt atcttggaca ggaggatcgt ttcggcgagc
    11461 tttatcctga ggtggagcaa tctgatatga tcgctattac tgcctattat cctgatacgc
    11521 ttgatattga ggcggttaag gctgatctgg ctgatcgttt ggctaatttt gaaggctttg
    11581 gtcttgcgac gggtagcgtc aaccttgaca gtcaggaatt ggttgaagag gactgggctg
    11641 ataactggaa gaagtactat gagccagctc gcattaccca tgatttgacc atcgtgccgt
    11701 catggacgga ctacgaggct aaggctggtg agaagattat caagctggat cctggtatgg
    11761 cttttgggac tgggactcac ccaacgacta agatgagtct ttttgccttg gagcaagtgc
    11821 ttcgtggtgg tgagacggtt atcgatgtgg ggactggttc tggcgttttg tctattgcta
    11881 gctctctctt gggtgccaag gacatctatg cttatgattt ggatgatgtt gcggtgcgcg
    11941 tggctcagga aaatatcgat atgaaccctg gtactgataa tattcatgtg gctgctggtg
    12001 acctgctcaa aggtgtccag caagaggcag atgtcattat tgccaatatt ttggcggata
    12061 ttttgatcca tctgacagac gatgcctacc gtttggtcaa ggatgagggt tatctcatca
    12121 tgtctggtat catttctgaa aaatgggaca tggtgcgtga gagtgctgaa aaagctggtt
    12181 tcttccttga gactcacatg gttcaaggtg agtggaatgc ctgtgttttc aaaaagacag
    12241 atgatatttc aggagtgatt ggcggctaat gcaacagtat tttgtaaatg gcaaagcaga
    12301 tgactttgtg accatcgagg acaaggatac catcaagcac atgttcaatg tcatgcgatt
    12361 gacagaagat gaccaagtgg tcttggtttt tgatgatgcc atcaagcgtc tggcaaaggt
    12421 ggtggacagc tctgcccatc gcttccaaat tctagaagag ctagacagca atgttgaaat
    12481 gcctgtccaa gtgaccatcg cctcaggttt tcccaaggga gacaagttgg attttgtgac
    12541 gcaaaaggca acagaattgg gtgctgcggc tatctgggga ttcccagctg actggtcagt
    12601 cgtcaagtgg gacggcaaga aactagctaa aaaagaagat aagctggcaa aaattgctct
    12661 gggcgcagcg gagcagagca agcgtaatcg tcttcctcag gttcgtttat ttgagaagaa
    12721 ggctgatttt caagcggagc ttgcaggctt tgataagatt tttatcgcct atgaagagtc
    12781 tgccaaggag ggtgaaatgt cagcgttagc ccagaatttg cagactgtaa aagcaggtga
    12841 caagctcctc ttcatctttg gaccagaagg gggcatttct ccaaaagaaa tcgcagcttt
    12901 tgaagaggtt ggagctatca aggtgggcct gggtccacgc atcatgcgca cagaaacagc
    12961 accgctctat gctctaagtg ttattagtta ctcagcggag ttattatgat tattgctatt
    13021 caaggtgata tggctgtagg gaaaaccaca gctttgaaaa tcattgagaa agtttacccg
    13081 aaaatcgcta ttagttttga ggatatttca ccagtagttg ataagattaa taaacatcag
    13141 cttgataaca ataaattaga cgattattgt gaaattcaac aactatttat tcagcatgag
    13201 cgagatagat acgaacttgt tagagataat gaaatagtgt taatggatta tggagctgag
    13261 gaaattgctt tctatacaca atactttcca aaggtggcgg gttatagttg actttacctt
    13321 ctaatttgtt actagaggta aataggctaa aggaatgttt tgctgattat attatttttc
    13381 tagatgcaga tgagacggtc ctataccaac gtcaaaaaaa tgattttaat agacggcgtc
    13441 attttttcaa atggtatact catcacctgt tgcctgctaa aaaacattat ttagacacat
    13501 tttccaatgt tattcatatt aatacaaatc atttaaatgc tgaagaagtg gctgaaaaaa
    13561 tcattgacat cattcgtaag ttaactctat aatgggatag ttatacttca attatgtggt
    13621 ataacaactg tttcaagccg acttattggc tgaattacca taatagaaaa aaggagtttg
    13681 gataattttc caactctttt taattttaca ttgattttac aaaaatcctc aaagagattt
    13741 gatattcctt aactataata aaatcaaatc aaaaaatatt taggagttca tcatgaaaaa
    13801 acacaaaatg ctatctcttt tggcggtatc tggtcttatg ggaattggca tcctagcagg
    13861 atgctcaaat gattcatcat cttcatctaa agggacaatt aacattgttt ctagagagga
    13921 agggtctgga actcgtggag ctttcataga gttatttggg atcgagtcaa aaaataaaaa
    13981 gggcgaaaaa gtagaccata cttctgatgc agcaactgtt accaattcaa cttcagttat
    14041 gctaaccact gtctctaaag acccgtcagc tattggttat tcttcactag gttcattaaa
    14101 tagtagcgtt aaagtgctta aaattgatgg caaaaatgcg actgtaaaag atattaaatc
    14161 aggaagctat aagatttctc gtcctttcaa tattgtaaca aaagaaggta aggaaaaaga
    14221 agcaacaaaa gattttattg attatatctt aagcaaagat gggcaagcag tcgttgagaa
    14281 aaatggctat attccacttg acaatgctaa agcatatcaa gcaaaagtgt catctggtaa
    14341 agttgttata gcaggttcaa gttcagtaac gccggttatg gaaaagatta aagaagccta
    14401 tcataaagta aatacaaaag tagatgttga aattcaacaa agtgattctt caactgggat
    14461 tacaagcgca attgatggat cagcagatat aggtatggca tcacgtgaat tggataagac
    14521 agagtcatcc aaaggggtca aagctactgt tattgcaaca gatggtattg cagtagttgt
    14581 taataagaaa aataaagtta atgatctatc aacaaaacaa gtaaaagaca tttttactgg
    14641 aaagacaacg tcttggtctg acttatcaaa gtaaggtaag agtaaacaat gaaacaaaca
    14701 aaagaaggtc ttatgcaagc tgtgtttttc atcacggctt gcgtcagtgt tatatcagct
    14761 attttaattt gcctctttct cttctctagt ggtcttccag ctattacaaa aatcggctgg
    14821 ggaaatttta tatttggtaa agtctggcat cctagtaata atatttttgg catctttcca
    14881 atgattgtag gttccttata tgttacagcg ggagctttac ttttaggagg cccgataggt
    14941 atccttactg ctgtttttat ggcttatttc tgcccagaaa atatttatag gcctttaaag
    15001 tcagctatca atttaatggc gggaattcct tcagttgttt atggtttctt tggactcgtt
    15061 gtcattgtcc ctatgataag gcaatatatt ggtggctttg gtatgggagt cttagcagct
    15121 tcaattttgc taggaattat gattttacca acaattgtta gcatttcaga atcttcttta
    15181 agagctgttc cagagagcta ttatgaaggc ggtattgccc ttggtgcctc tcatgaacga
    15241 agtgtcttct ttgctgtttt accagctgct aaacgtggta ttttagcatc ggttgttttg
    15301 ggaattggac gtgctattgg tgaaacaatg gctgtcatca tggtagcggg taatcaagct
    15361 gtcttgcctc aatcgctgac atcaggcgtt agaactctaa ccactaatat tgtcatggaa
    15421 atgggatatt cttctggttt gcatcgtcaa gccttaattg gtacagctgt ggtactcttt
    15481 atctttatat taatgattaa tatcagtttt tcagcattac agaaagagga ataagatgga
    15541 aaatattaac caaagaacgt caaaacaata tttagcatct ttaaaaaaag atcccctttc
    15601 cttctttctt tttgctattg tctatttggg agcaattcta agttttgcta caattgcatt
    15661 tgttgttatt tatattttag ttaaggggct tcctcatgtg aatactggct tgtttgcatg
    15721 gacttataat actcagaacg tctcactatt accagctttc attaacacta tctttatcat
    15781 cgccttaact ttattatttg cagttcccct tggtattggt ggatcaattt atctaacgga
    15841 atatgctaga cgtgataatc cttatctcaa aattatccgt gttgcaaccg agacattggc
    15901 agggatacct tcaattattt atggtttatt tggtgcatta ttttttgtaa aatacacaca
    15961 cttaggctta tctttgatat cgggttctct gactttgagc atcatgattt tacctttgat
    16021 tatgaggaca actgaggaag ctttactatc tgttccggat tcttatcgag aaggagcctt
    16081 tgcgcttggt gctggtaaat tgagaaccat atttaaaatc gtattaccta gtgctatgtc
    16141 tggtattttt gctggtatta ttttagcgat aggacgtatc attggtgagt cagcagcact
    16201 tattttcaca gctggaacag ttgcaaaagt agtacattcg gtatttagtt catctcgtac
    16261 tttagcagta catatgtatg ctatttcggg tgagggctta tatgttgacc aaacttatgc
    16321 gactgcggtc atcttattgc ttttagttat tattgtcaat tttgtatctg gactagtagc
    16381 caaacgatta ggaagtgagt cgaacagtga gtaaattagt aattaataat ttagatttgt
    16441 attatggaga atttcacgct ctgaaggatg ttaatttaga cattgaagaa aaagaaatca
    16501 cagcctttat tggaccttct gggtgcggaa agtctactct cttgaaaagt attaaccgta
    16561 tgaatgattt ggtcaaaaat tgcaaaatca caggagatat tactttagaa ggcgaggatg
    16621 tctatagaca attggatatt aaccaattgc gtaaaaaggt tggaatggtg tttcaaaaac
    16681 ctaatccgtt tccaatgagc atttatgata atgttgcttt tggtccaaga acgcacggta
    16741 ttcattccaa agcagagtta gatgatatcg ttgaacgttc ccttaaacag gcggctctat
    16801 gggatgaggt taaagatcgt cttcacaaat ctgctctggg tatgtctggc ggacagcagc
    16861 aacgtttgtg tattgcgcgt gctcttgcta ttgagcctga tgttttactt atggatgaac
    16921 ccacaagtgc cttggatcct atttcaacag caaaaattga agaattggtt atccaactga
    16981 agaagaacta cactattgtt atagtgacac ataatatgca acaagctgtt cgtatttctg
    17041 ataaaacagc attctttctg atgggagaag tggttgaata taacaaaacg agccaactct
    17101 tttcattgcc acaagatgag cgtacagaaa actacattac agggagattc ggatgattcg
    17161 ttcacgattt gcaagtcaac ttaatgacct taataaagaa attattttta tgggggcatt
    17221 gtgtgaggac atcattggga agtccttagg tgctctaaca aatagtaatg atgtttacct
    17281 tgacgatatt tctgaaactt atcacaagat tgaacaaatg gaacgggata ttgaggagag
    17341 atgtttaaaa cttttacttc gtcagcaacc agttgccaag gatttacgcc gtatttcttc
    17401 tgcattaaaa atggtttatg atatgaaacg tattggtgct caagcttatg aaattgcgga
    17461 aattgttagt ttaggtcata ttatccaagg tgcaggttct gaaagggatc aacttaacag
    17521 catgtctaat aatgtcatta gtatgctgac taagagtata gatgctttta tttatgacaa
    17581 tgaagaacaa gcgcatcaag taattgaaca agataggaca gttaaccagg aatttgatac
    17641 aataaagaaa cagttagtgt tatatttttc ggtacaggat gttgatggtg aatatcccat
    17701 agatgtttta atgatcgcca agtacctaga gcgtattggt gatcatacag taaatattgc
    17761 taaatgggtg cttttctcaa taacaggtaa cctagatgga gaagcgtaat gatatattgt
    17821 gttgaagatg atgctgatat tcgcgaaatg atgctctata ctttgcaaat ggcaggtttt
    17881 acagctcaag gattctcgag ttcagagctt ttttgggaag ctgttcagga gaaagttcca
    17941 gacttgattt tacttgatat tatgttgccg ggagatgatg gtttgactat tttagagcgt
    18001 ttaaggagaa aacatcaaac ggaaatgata ccagtaatca tgacaactgc taaaggtagt
    18061 gagtatgata aagttaaagg gttagatctt ggagcagatg attatctcgt caaacctttt
    18121 gggatgatgg aaatgatttc acgaataaaa gcagtcttga gacgtagccg ccaagtagat
    18181 tcaaaagctc atattatcat tggaaattta gagattgacc cgactaatta ttgggtcaaa
    18241 aggggaacgg aaaaaatcca cttaacccta aaggaatttg agttattagt attattcttc
    18301 cgtaatccca atagagtctt tacaaggcaa gaacttctgg ataaggtttg gggagaacaa
    18361 tttttaggag aaactagaac tgtggatgtt cacattggga cactgcgaac aaaacttggt
    18421 gaggatggct atttgatcgc tacagtaagg ggagttggat accgtttgga ggaaagacat
    18481 gactaaaaaa attttccgta caactttatc cgctagtttg ggaattgttt tagttacaat
    18541 tttgatgatt atgggatttc tctataatta cttcaatcgc attcagcgag aacagttgag
    18601 aacacaaacg gcactggcgt cacaaggaat ttcctttgaa ggtaaagatt attttgaaaa
    18661 tcttaaaact tcaaatgtgc gtattacttg ggtagataat aagggacaag tgctttatga
    18721 tacccaatca gatgcaaaac atatgaaaaa tcacgccaat cgtcaagaaa ttaaagaagc
    18781 tatcaagagt ggttatggtg agagcacgag gtggtcagcg acacttactg agaagtctat
    18841 ctatgctgcg caacgtttaa ataatggaac gatagttcgt ttatcagttg cgcaacaaac
    18901 tattttttat cttctccttg gtatgatgag tcccttagca attatcatct tacttgctat
    18961 tattttatct gtattaattg cacgatatat agcaaaaaaa gtatcagagc ctttaaacaa
    19021 tattgactta gatcatcctt tatcaaatga tagttacgag gagataacac cattattaag
    19081 gcgtcttgat agccatcagg ctaagattca acatcaaaaa ctgttattac aaaagcgtca
    19141 aaaagaattt gataccatta tttctaaaat taaggaagga atgattcttt tagatgatca
    19201 agcaagaatc gttagtatca acgcagaagc tttaaagtta ttccaaatta atgatgattg
    19261 gcatgggcgc tttatgatgg aagttagccg agatttaact ctcaaagact taattgatca
    19321 aggactaaaa gggaaaaaga aagaggctaa tattgacatt gaaaataatc attaccgcgt
    19381 cttggtaaga ccaactacag ataacaaccg agtaacagga ttggttgttc tactgtttga
    19441 tgtgactgac caactccaaa tggaacaact tcaacgtgaa tttactgcca atgtctctca
    19501 tgaattgaaa accccgcttc atgttatttc tggttactca gaactgttag ctaatcaaat
    19561 ggttccaaac gaagaagtcc cacaatttgc agctaaaatt cataaagaat ctgaacgatt
    19621 agttaaattg gttgaggata ttatcaacct ttctcacctt gatgaacaag aaaaattacc
    19681 gcaagagaca gtaaatctgt atgacttaac acaaaaagtt ttagaaggtt tacaagctaa
    19741 agcagataag aagcacatac aaatcaattt taatggagaa gaagctattt tgcgagggaa
    19801 tccagtttta ttgaattcct tggtgtacaa cttgtgtgac aatgctatca cctataatca
    19861 tgagaaaggt caagtaaatg tgactttgaa aaattcacct gatacaatta ccttagaagt
    19921 tagtgatacg ggactaggca ttgcagagaa ggacaaaaaa cgtattttcg aacgtttcta
    19981 tcgcgtcgat aaaagtcgct caaaaatagt tggagggact ggtttaggtt tgtctattgt
    20041 aaagagtgct ttagattttc acaatggtag tataaaagtg gacagccatt tggggcaggg
    20101 aacaacaatg accgttttac ttcacaaaca ataaaaactc acaaataaaa gtctagatga
    20161 tattatctag acttttttag taattcagaa aaaatatata agtaagggtt tacaaaaaac
    20221 aaataagtgc tataataaaa ccataacgca aacgtttgca ctattaagtg cataaaagga
    20281 gattgaagat gaaaaataat gtcaaacagc tctttagttt tgaattttgg cagaagtttg
    20341 ggaaggcttt gatggttgtt attgctgtta tgccagcagc aggattgatg gttagtattg
    20401 gtaactccat taggctattg gatccatcaa atgttttatt agggcgcata gctaatgtta
    20461 ttgctcaaat tggttgggga gttattggta acttacacat cttatttgca ttggctattg
    20521 gtggtagctg ggctaaagaa cgtgccggtg gtgcttttgc ggctggctta tcctttattc
    20581 ttattaacct tattacaggg aacttttttg gtgtgaaaac agatatgtta gcaaacagta
    20641 aagcaactgt tcaaacggta tttggtgcga ccatcagagt ttcagattac tttgttaacg
    20701 ttcttggaca acctgctctt aatatgggtg tatttgtcgg tattatttca ggtttcgttg
    20761 gtgcaactgc ctttaataaa tattataact atcgtaaatt accagatgct ttaacttttt
    20821 tcaatggaaa acgttttgtt ccatttgttg ttatttatcg ttcagtcatt gttgcattaa
    20881 ttttatcagt attttggccg gttgttcaat caggtatcaa tggctttggt aagtggattg
    20941 caagttcaca agacagtgct ccaatattag caccatttgt ctacggtacg ttagagcgtt
    21001 tattgctccc atttggttta caccatatgt taactattcc aatgaactac acgcaactag
    21061 gtggcacata cactgtcctt acaggtgcaa ctaaaggagc acaggtgttg ggacaagatc
    21121 cactgtggtt agcatgggtt ggtgatttaa tcaacttaaa aggctcaaat tcaagtcaat
    21181 accatcactt attgacaagt gtcactccag cacgttttaa agtggggcaa atgattggtg
    21241 cttcaggtat tttgatggga ttatcttatg ctatgtatcg taacgttgat aaagataaaa
    21301 aattgaaata caagtcaatg tttatttcag cagcagcggc tactttcttg acaggtgtta
    21361 ctgagccaat tgagtacatg ttcatgtttg cagcaatgcc actctacctt gtatatgctg
    21421 tcgttcaagg atgtgcgttt gctatggcag atattgttaa cctccgcgtt cattcatttg
    21481 gtaatataga atttttaaca cgtgttccca tgggaataaa agcgggcctt ggtggtgata
    21541 ttttcaactt tgtttgggtg acacttcttt ttgcagtatt gatgtacttt attgcaaact
    21601 ttatgattaa aaaattcaat ttagcaacag caggacgtaa tggtaattat gataacgaag
    21661 aggttgataa tgctccttca acagcttctg gttctgctga tgcaaattct caagttgtac
    21721 aagttattaa tttacttggt ggtcgtgata acattgaaga tgtagatgca tgtatgactc
    21781 gtcttcgtgt cacagttaaa gatgggaata gcgtgggttc tgaagcagct tggaaaaaag
    21841 ctggtgcaat gggattggtg ttaaaaggta atggcgttca agctatttac ggacctaaag
    21901 cagatgtcct taaatcagac attcaagatt tgctagattc aggtacagtg attccaatag
    21961 ttgatttaga aacaggccag cctgtagcag caacccaagt tacaacttat aaaggaataa
    22021 cagaagaaat tgtttcggtg gctaatggtc aagtagaagc attggatgtt gttaaagacc
    22081 ctgtcttttc tcaaaaaatg atgggtgatg gctttgctgt ggaaccaact gatggcaata
    22141 tctatgttcc agtttcaggt acagttacta gtgtctttcc aacaaaacat gcttttggtt
    22201 tattaacaga aagtggccta gaagttttag tacatatcgg tctagataca gtagctcttg
    22261 atggtcaacc atttgaggtt aaaatttcta gtggtcaaaa agtggtagca ggcgatcttg
    22321 ctgtagtagc agatttggaa gctatcaaag cagctggtaa agagacaagt gtgattatcg
    22381 tctttacaaa tgtttcagat ataaaaactg ttaaacttga aaaatcaggt ccacaaatag
    22441 ctaaaacagt tgtggcaaaa gtcgaactgt aacaaacgat aggagctggg caagtctcag
    22501 cttctttctg atgaaaggat actctatggc aaaatgctta acgttaaata ctcattcatg
    22561 gatggaagta aatgctctaa agaaattatt tgatttagca gagcatatct tccgtgaaaa
    22621 atatgatatc atctgcctac aagaggtgaa ccagagcata tcttcccctc ttgctacgag
    22681 tagtccaaat tatcatccga ttgaaggtac accagctctt catcaagata actttgcctt
    22741 gcaattagtg cattacctaa atcttcaagg tctacactat cattggacat gggcttataa
    22801 tcatattggt tatagtaagt accatgaagg ggtagcgata ctttctctga aaccattaaa
    22861 gccagatgat attttagttt cagctgtaga cgatgagact gattatcata ctcgtcgtgc
    22921 tctggtagct gagaccactc tgaatgacaa agtcgttaca gtagttagcc tccatttatc
    22981 atggtttgag aaaggatttg cggaggagtg gaaacgacta gaaactaccc tacttgaggt
    23041 agaaactcca cttttactca tgggtgactt taataatcct actggaaatc agggttatga
    23101 acttgttctt aatagtccat tagcattaaa ggatagccac caaatagcca atcatgtatt
    23161 tggagaccat actattatgg ctgatattga tggttgggaa gggaataaaa aggctcttaa
    23221 agttgaccat atttttacta gcgaagacct ttcaatcagt tcaagtcaag tagtttttga
    23281 aggtggtgaa gcacctgtcg ttagtgatca ttacggcttg gaaattacgt taaatcttta
    23341 gattaatcta tttaatatat taaaagcatc ctaaatttgt ctataaaaca aaacttaggg
    23401 tgctttttga aaaatatgtc acattattta acttcttacg aagtttggaa tgcatgagaa
    23461 aatatgataa aatgataata actattatag gtgattttaa taaataattt aatcaattgg
    23521 tttatctatt agaacaatta atacgaggag acgagagtga atatttttat tttagaggat
    23581 aattttattc aacaggcacg tttagagaga ataattgagg aagttaaaat taggagaagt
    23641 atacacagta aaatggtaaa tacctttgaa aaaccagttc aattattgga atcaatctgt
    23701 gaaaaaggta tgcataattt atatttctta gacattgaaa taaaaaatga tgagcagagg
    23761 ggcttagagg tcgcaaaaca aattagacaa attgatcctt atgctcagat tgtttttgtt
    23821 acaactcatt cggaattaat gcctttaacc tttcgttatc aggtatcagc tttagactat
    23881 atcgataaag gactttcaca ggaagcattt agcagaagga ttgaagacgt tttactatat
    23941 gctaataatg cttgcagtaa aaacttacta gaagattctt tttattttaa atcacgctat
    24001 agtcaagtac aagtaccttt taacgatatc ctttatatag aaacgtcatt aaggtcacat
    24061 agagttgtcc tttatactgc aaaagacaga atggaattca cagccacttt gggggatatt
    24121 ttaaaacaag aacctaggct ctttcaatgt catcgctctt ttttagtaaa tcccttgaat
    24181 atttttaaag tggataggtc aaataaacta atctattaaa aaaatggttc gaattgtttg
    24241 atttctcgta ataaaataaa agatattatg gtagtaatag aggaaaatca aagaggtaga
    24301 ggaatatgaa ttctactcta tccatcttag agatagcttt aacgataata ttaacaatat
    24361 ttctcttttc taaaatcagt ggacgtcaat tgactttaat agagattgga atttgtattg
    24421 ctctgagatt aggacttgta attttattta tagcagctaa tactattatt aatctttcat
    24481 ttctaaagta ctggcttctc cccatctata tgttaagtct ggctttcttg ttgctaagac
    24541 ctatatctaa aactttagta gtcttttatg gcttgtttcc aattgttctc agaaatcttt
    24601 gttcacgttc tttagctttt tttgtctttc ctttgtttaa tattgatttt catacaacca
    24661 accaggggtt ttggggatat tttgttgatg tcttgtctac tctttgtgtt ctcctatttt
    24721 taagatggat gcaatacgac tttaaaactt tgcgaagtag tgaagtttcg cccatagata
    24781 agagaattct ccaatttacg aatggttgta tggtcagtta ttacttactg atgcagtttt
    24841 caacttattt ggaatatgaa ttagccatca atactgttat ttaccggcaa tttttgcttg
    24901 ttgtttattg gattatattt acgggagcaa tagccagttt agaccggaga cttcgaaatt
    24961 ctttacagga gaggttagct gttcaaagag aactccaatt ggctaatatg atagattata
    25021 gccaccatat tgaggggttg tataaagaag ttaggagctt ccgacatgat tatagtaaca
    25081 tgttaacgac cttgaaacta gggattgata atgaagatat atctattgtc aaatcagttt
    25141 atcagtctat ctttaaagaa tctaatacac atttccgaca tcctaagtat aatatagacc
    25201 ggttggttaa tgtagataac ccagccttaa aaagtctttt agcagccaag ctttcacaag
    25261 cgatagacaa tcatattatg gtatctatag aagtaccaga cagtattaaa cctcaaggga
    25321 tggaactgat tgattttatt accattgtat ctgttttatg ggataacgct attgaggcat
    25381 ctttagagac agacgatcct aagatagcca tgtcttattt tacattaaaa cataaacaaa
    25441 tttttatcat agaaaattca actaaggaaa aggcaataga tacgtcaacg atattcaatt
    25501 atgagataag tagtaagggg cggaatagag gaataggtct ttacaatgtc aggggagtta
    25561 tcaatcgtta tccaaatatt tctattgcaa caagtagcca agattttagt ttctgccaaa
    25621 ctttcgaaat catattatag ttttaaaaag ttcaatttat ctaagtttat tattttctta
    25681 ataccatcaa tccaaccacc accagtgata ttatttagtt gtttcggcga taattcctga
    25741 agctgtagtt ccatctcgat tagttttggc tagttctcgt aagtggttta ataaatattt
    25801 tgaaccatag aactttgcga ttgaagcaag tgcagttact ccacaatcct tagtatcaac
    25861 ctgacgcacc aatgcttttt tgtacgtcaa cattgtgtaa ttcctttctt cgttatcgta
    25921 ttaatatttt gccataaaaa tatatactta ttaaaaatag cttaaacagc tattgcaacg
    25981 cgtccaatgg tttctataag gatataaagg tagatagtac ttttctgttt tgaggcgatt
    26041 caacgacatt tcagggaata aacgtttcta tttttatttg aatgctatat taaatgtgtc
    26101 aagttgcaac agacaaaaat tttatatagg agatattcaa aataaatgta aaatcaattg
    26161 aacaattaga agttatggat aatgaagcat tatcacatgt tgaaggtggc cttggtcata
    26221 gatgggtata cagtaagaac cggtaaacat acttatacaa gtgcatggca tctttctgaa
    26281 aatactgctg cagcaaatgc aagaaatcgt atgggccttt accctggaag cgtttgtcgt
    26341 gtctatcttg tataattgat tttttattta agcgataggg aatcttcctt gccgcttaag
    26401 tttgtatagg gagagacaat actatgttat tacagctaga taatatcact aaaagctacg
    26461 gaaaaaaaat agtacttaac cagatttcct accagtttac tcctggtttg tatggactct
    26521 taggagcaaa tggtactggc aaaacaacct tattaaattt gatgagccac tttacattcg
    26581 cagatagtgg gaatatttat tggaatggac aggagcagtc ggaagaattt tatcgtcaca
    26641 ttggtttttt accgcaacat tttcgctatt atgatcaatt tactggcatt gctttcttaa
    26701 attatatcgc taccttaaaa ggtgttgata aaaagaaagc taaaccagaa attcctagat
    26761 tactagagct tgttggttta ggtgatgtag gtaaaaagaa gatttcatca tattccggtg
    26821 gtatgaagca acgtttaggc attgctcaag cacttatcaa tgaccctgaa atacttattc
    26881 tggatgaacc aactgttgga cttgatccta aggaacgggt taaatttcgc catattttga
    26941 gtcagttatc caccaataaa attatcattt tatcaacgca tattgtgtcg gatgtggaag
    27001 ctgttgccaa agaaattata gtattaaaaa atggtaaatt tatcgaacag ggcaacaccg
    27061 cacagttgtt gaaaactatt gagggaaaag tgtgggaaat aacgactgaa ccagatttat
    27121 ctcagatacc caatattgct attgttaatg aaaaagtcat ttcagatagt ctggtatttc
    27181 gtgtagtgag tgatatttgt ccgagtgatt cggcacagtt ggttgttccg actctagaag
    27241 atttctacat ctatcatttt agaggggagt aagtaagatg atatcaattt ttccttttga
    27301 attaaaaaaa cttctacaac gtaaggcagc attgggggct tttattatct tccttcttgc
    27361 tatttttggt ctattttacc agcatttttt tgttgggcaa attagtggct attctgcaga
    27421 taaaatacat ggtcgagaag cggttgcaat taatcgtaga atagctgaaa aatattcagg
    27481 ggatttatct gaagaacgag taacaaatat tattaaaaac tatgttaatc cgccagcagg
    27541 acgcccccct atatttgatg tttttagtta ttatgtggca gacacttttg atcgtgattt
    27601 tttaatttac tataaccaat tgaaagatga taaaacaatt gattttaata aaataaatat
    27661 taaagatcaa aaacagttgg gttctgcgct accactaaaa caactaaagc taggtaactt
    27721 tgctccttgg aatcaacttt ttgaagtaag taatagtact tatattttaa ttgtcttatt
    27781 tactctattc ttatcagctc ctcttttttc aggagatagg gctaagaaga ttaatcctat
    27841 actactgacg actcggtatg gacgtggtca gttgacctgg gctaagttag ctagtacaat
    27901 gaccatcggt atttccacat ttatcctagc atatgccctc attctgacgg tatttgcaga
    27961 ttattttggt tttagtggtt gggagacaag tgttcaactg aatttatact ggacatcacc
    28021 tttggctaat attatgggat ttcctgtacc tatgacaata tcaactgctt ttgttcatat
    28081 tattgtctta caatttgttg gactcctttt tatagccagt cttaatttct ttatattaag
    28141 tatcacaaat tcgccattaa catcttttgc tgtttcaacg cttgcctttt ttgctcctag
    28201 ctttttaatg gctatgttta ataaaggtgt gataaacaag ttattaacta ttttctcagt
    28261 atcaacgaca gacacttcag ctttactttt aaaattatca cgtaatggta gtcatggctt
    28321 tttctttgat agtttttctg ctaatgaaat ctgtctgatt agtctacgta tgttaattgc
    28381 agtgttattg atagcttata cctatcgttt aatgcgacga agaagtttat agttcccact
    28441 ggttatctga acaagaaatt attctaagct gattttcttt tgcgaaatca gcttttttat
    28501 ttcctaatat ggttgataag gtattaacaa taattatcag ataccattta aggcgtttca
    28561 atgacttttg gggataattg agatattctt ctgaagagat tggtatcata gtaatgataa
    28621 cttatagaaa gtaatgttaa ggatgaagag aagtcagtgc atggataata agcagcataa
    28681 tacatcaatg attagcttgc tacaatatct tttttccata ctggtcattt tagttcatag
    28741 tggaaggtta tttagtcagg atgtcattca ttttaccttt aaaagtttcc taggtcggat
    28801 ggctgttcct tattttttga tttgtactgc tttctttttg agaggtcgta tacagcaagg
    28861 tttgtgtaat cactcctact ttcgtaaact gattaaaaag tatagcatgt ggactatcat
    28921 ttatttgcct tatggttatt tcttttttga atctctaaat atagcgaaga tttatttgct
    28981 gccgggtttt attgtggcat ttttgtattt aggtatgtca catactcttt ggtacatccc
    29041 tgctgttatt ctaggttggt tcattattca aggattattg aagtatgtag gaactagggg
    29101 gacatttatc acagttgttg tactctattg tattggagca gtagagacct attctgtctt
    29161 tgtacagtca acaaaatttt atcccctgat gtccacttat atgtcaattt ttcagacaac
    29221 acgaaacgga ttattttaca ctccagttta tcttctagca ggatatttac tctatgatta
    29281 ttttaatact gacctattta ccaaatcgcg tggtcttaag tatattctct tccttttact
    29341 tctagcttta gagaatgttc ttatttattt taatcaaggg ttggataaaa atttcttctt
    29401 attagctcct ttatgtgcag tattcttatt taactggagt atacgtacat cactgtttaa
    29461 gggctatcgt ttaagtcctt taaaacagtt gagtgtctat tatttcttcc ttcaccctct
    29521 ttttgttggc atagtgtctt actgtttaaa atcaacttcc ttagttgcac accatcaagg
    29581 aaaagtgatt tttgtggtga ccttggcatt aacacatgct tgtgctctac tcataataaa
    29641 ggttgttaat aagataccct atcaaaaaat tattccatta tctctttcga aatagtgatt
    29701 catataagag aaagtttaaa gttatagtga tgtaaaggtt gtgtaaacta ttaggataca
    29761 tgaccttttt attgttctgt ttttaaatat taattatata gaatagtcca cacatagagt
    29821 agaatagtcc atttttaaac cgttttcatt tgttagaatg atagtaaagg ttatcttatt
    29881 caaagtattg ataaaacttt cagaactaat ttagggtaat gtaataaata ggaggagaaa
    29941 gatgaagcgg aaattcctaa tcggcagtca cggaaaactt gcgagtgggt tgcagagttc
    30001 tattgatatt ctgacaggca agggacaaga gattcagaca atagatgctt acattgatga
    30061 tagtgactat accaagtcta ttgtagaatt tattgatgag attgcaccag atgaacaggg
    30121 gcttattttt acagatttgt taggtggcag cgttaatcag aaaatggcga ctgctgtaat
    30181 gaatagtggg aaaaataatg tttttttaat aacgaacagc aatttagcga ccttgttgtc
    30241 attactattt ttgaaacctg aagaggaatt aactaaagaa gaaattgtaa ccgttatcaa
    30301 tgaatctcaa gtgcagctgg tagatctcag ctttaaagcg tgttcagaag atgacttttt
    30361 cgattaataa ttaggaggaa aaaaatgatt actcaaattc gtgttgacga ccgtttgatt
    30421 cacgggcaag tggcagttgt atggactaaa gaattaaatg caccactttt agtggtggct
    30481 aatgatgagg ctgccaaaaa tgaaatcaca cagatgactc ttaaaatggc tgtgcctaat
    30541 ggcatgaaat tattaatccg ttcagttgaa gaatcaatag ctctatttaa agatccacgc
    30601 gcaactgaca agcgcatttt tgtgattgtc aattctgtta aagatgcctg tacaattgct
    30661 aaaaatataa ccgatttaga agcagtaaac gtcgctaatg taggacgctt tgataagtct
    30721 gatccagcta caaaagtcaa actaacatca agtctcttat taaatccaga agagttggaa
    30781 gccgcaaaag aattagccag cttaccagat ttagacgtct ttaaccaagt attgccttca
    30841 aatactaagg ttaacttgag tcagctagtc aaacaaagtg aggtatgaca tatgataatg
    30901 ccagcgacga tggcagcttt agctgtgttg atttgttttg ggggaaatta cttaacaggt
    30961 caaagtatga tggagagacc acttgtagtt ggtcttgtaa caggaatgct actaggtgat
    31021 attaaggttg gtatcttaat gggtgcttcc ctagaagcgc tatttttggg aaacgttaat
    31081 attgggggtg ttattgccgc agagccagta acagcaacag cgatggcaac aacttttacc
    31141 attatttcaa acattgatca aaaagcagca atgactctag ctgttccgat tggaatgttg
    31201 gcagcatttg ttgttatgtt tttgaaaaac gtcttcatga atatctttgc accgatggtg
    31261 gataaagccg cagccgcaaa ccatcaaggt aaacttgtga tgcttcacta tggtacatgg
    31321 attatttact atctcattat cgcttctatt tctttcattg gtattttagt aggtagtggg
    31381 cctgttaata gttttgtcca tcatattcct caaaacctga tgaatggctt gagtgcggcg
    31441 ggaggcctcc ttccagctgt tggttttgcc atgctgatga aattattatg gacaaataaa
    31501 ttagctgttt tttacttgct tggttttgtt ttaacagctt atttaaaatt accagcggtt
    31561 gcagttgctg ccctaggagc agttatttgc gtcattagtt cacaacgtga tatagaatta
    31621 gacgcaatta ctagaggtgc gatcagtaaa cagacaacct ttgactctaa agaatcagaa
    31681 gaggaggact tcttcgcatg aaatcacagg ataatttgac taaagaagac cgtaaaatgc
    31741 ttcgctcagt cttctggcgc tcatggacga tgaatgccag tcgtactggg gcaacacaat
    31801 accacgcagt aggagttatt tatactttac taccagtcat caatcgtttt tataagactg
    31861 ataaggataa agcagaggca ctggtccgtc atacaacatg gtttaatgcc accatgcaca
    31921 ttaataattt tattatgggt cttgttgcat ctatggaaaa gaaaaacagt gaagatcctg
    31981 attttgacgc tagtgctatc accgcagtta aggcttcttt gatgggacct atatctggtg
    32041 ttggtgattc attcttttgg ggaattctaa gggttattgc ggcaggtatc ggtatctcct
    32101 tagcaagtac aggctcagcc atgggagctg ttgtctttct actcttgtat aacattccag
    32161 cttttctgat tcattattat agtctctatg gaggttactc tgtaggtgca gggtttatca
    32221 aaaaattgta tgaatcaggt ggtattaaaa ttgttactaa gacttcaagt atgcttggtt
    32281 taatgatggt aggctcaatg actgcttcaa atgttaaatt caagacaatc ttaacggtag
    32341 cagctaaagg agctaaggaa gcagcttcca tacaaagtta ccttgaccaa ttatttgttg
    32401 gcgtagtgcc actgttggta acgatattag ccttctggct tctacgtaaa aaagtcaata
    32461 tcaactggat catgtttggt attatggtgt taggtattgt acttggtctc ttaggtattt
    32521 gctaataatc tcctagcatt aaaggtaagt ggaacaagac gatacttgtt tagcttatct
    32581 ttttttcttt tgaaagttat tgtatgaaag gagttgacta tctttattgg atagctattt
    32641 gctataataa aaggagatgg aaatgagagg atatagaatg gaagaacgct ttaagaaacg
    32701 actacaagat gatatctcta aacatttttc ccgccagtct ttaattttat ccttgttatt
    32761 gattgcactt ttcgtccttt tttctttagc gcctcagcag ataggccttt ataaagatgt
    32821 taattcagtt tcctatagtt ataagcaatt gattcaaaaa catgatacct tattagatga
    32881 cttaggtaaa aatagtttga aaccatttgt ctcaggacac ttaggaagtg ctgatttgag
    32941 taagcaatat tatcatttaa ggaatcattt acaaagtcaa acagaacttt tagtattctc
    33001 acctaatcaa gaacttttgt ttgctagtaa ttcacattta ggtaattttt ttagtaagtc
    33061 tatttacatt agtgaagttt tagataaggc taaaataaat caaaggctac taaaaattat
    33121 tgtagatagt gagggagggc attaccttgc actcattaag ccaattatag ttaataaaaa
    33181 agtttcaggt tatgcctttt tattgatgaa tgggaaagac tttttacttc caacgaaggc
    33241 tattaactca gacttgatta ttgccgatca gctaaataat agctttactt ttactaatcg
    33301 tgattttatc tcctctagtt tagataaggt agatagtcaa ttcttaacta gatattttag
    33361 ttttcacgat catcgagctt ttgttgttag gaaagtagct ctgcaagata atatcctact
    33421 ttatatgtat cgtcccctga taccagtaac tctggtcgtc cttttttctc tagtatcatc
    33481 agttattatt tttgtgattt tacgacgaaa atctagagtt ctagccgatc gcatcgcagt
    33541 aaaaaattct ggcgccataa atcaaatggt gctagatatg gatgccattt cccgccaaga
    33601 aaaatctagt attgagcttg atagtcaaga tgaatttcaa tatctatctg ttcaaatcaa
    33661 tcagatggtt tcacggttaa aagatttaca tgagaaaact ctcgatttgg aaacacaaaa
    33721 gttgctgttt gaaaagagga tgttagaagc tcagtttaat cctcacttcc tttataatac
    33781 ccttgagaca attttaataa ccagtcacta tgattcacaa ttaacagaaa gaatcgttat
    33841 acaactaaca aaattattgc gctatagcct tagtggtagc acagaggcag ctgtgcttaa
    33901 ggatgattta gcaatcattg aatcctacct tttgattaat caagtgagat ttgaagaatt
    33961 aacttacact atttcagtat ctcctgaact tgaacacatg cgtgtaccaa aacttttctt
    34021 gttaccgcta attgagaatg ctattaaata tggcttaaaa gaacgtcatg atgtagcaat
    34081 aaacatagat atttggcaag atagtgatgg aatttggttt actgtttcaa ataatggatc
    34141 gggtattagt ttggctaggc aaaaagctat acgtacaatg ttaagatcaa atcactcgca
    34201 tcatgggctt attaattctt atagacgatt acaatatcaa ttttcaacag tattgttaga
    34261 gtttacgaag acagatgatg cttttcgagt tagctatata gtaaaggagt gagttatgta
    34321 tagattactt atcgttgagg atgagcacct tattcgtaag tggttgcgct atgctattga
    34381 ttatcaatca ttgaatattt tagtggttgg tgaagcaaaa gatggtaaag aaggagctca
    34441 attaattcag gaagagcagc cagacattgt tttaagtgat attaatatgc caattatgac
    34501 agcattcgat atgtttgagg caaccaaagg acaatcttat gccaaaatta ttttatctgg
    34561 ctatgctgat tttccaaatg cgcaatcagc tattcactat ggcgttcttg aatttttaac
    34621 aaaaccacta gaaaagcaag ctttgattga ttgtttaaaa acaattatgg ctagaattga
    34681 ggaacataag gagaagcacc tacaagagca tacagaattg tatttacctc taccacaagc
    34741 taatgaccaa gttcctgaag ttattaaaga tatgctagca tggattcata gccattttca
    34801 tgggaaaatt gtgattagcc agttagctca cgatttaggt tacagtgaaa gttatcttta
    34861 cacagtcact aaaaagcacc ttcatataac tttgagtgat tatataaatc aatatcgtat
    34921 taatcaagcc attcagttga tgtttagaga accagaccta atggtttacc aaattgcaga
    34981 agctgttggt atttatgatt atcgttattt tgatagggtt tttaaaaagt acttgggtca
    35041 gacagtaaaa gcgtttaagg aagaacatat ttttaaacag atggattagg taggtgaaag
    35101 atgaaagaaa aacagtcgaa aaggcttatt tatatactac tgattgttcc cattatcttt
    35161 ataagtgttt ttacatacag tattagccag ccttctaaac tacttccacc aaaagaatta
    35221 gttattctaa gtccaaatag tcaagccatt ttaacaggaa cgattccagc ttttgaggaa
    35281 aaatacggta taaaagttaa gcttattcaa ggtgggacag ggcaactaat agatagatta
    35341 agtaaggagg gtaagcagtt gaaggcggat attttctttg gaggaaatta tacgcaattt
    35401 gaaagtcata aggcattgtt tgagtcttac gtatcaaaga atgttcatac tgttattcca
    35461 gactatatcc atccgagtga tacggcgaca ccttatacta taaatgggag tgtcttgatt
    35521 gtaaataacg aattagctaa gggacttacc atcaagagtt atgaagattt attacagtct
    35581 tccttaaaag gtaaaattgc ctttgcagat ccgaatactt cctctagtgc tttctcacaa
    35641 ctcactaata tactcttggc caagggtggt tacaccaatc caaaagcgtg gaactatgtt
    35701 aaaaagctac aacataatat taatgctatc aaatcttcta gctcttcaga agtttatcaa
    35761 tcagttgcag aaggaaaaat gattgtgggg ttgacttacg aagaccctag tgtcaatttg
    35821 caaaaaagtg gtgccaatgt ttctattgta tacccgacag aagggacagt ttttgtccca
    35881 tcttcggttg caattataaa gaatgctcct tctatgaaag aagcaaagtt atttattaat
    35941 tttatgcttt ctttagatgt tcaaaatgcc tttgggcagt caacgagtaa ccgacctatt
    36001 cgtaaagatg cccaaacaag taatggcatg aaagctttaa aggatatcgc tactcttaaa
    36061 gaagattatc gctatgtcac taagcataag agccaaatcc ttaaaaccta taatcgcatt
    36121 cgtagaaatg ctgattaaat gaaaatatag aaaaactcct cggaatcatt gattccgagg
    36181 agtttaatca tcgtcctcgt cgctttcgaa atcttttttc caataacgat aaagactaat
    36241 gcatgccaga aatccggcta taataggaac gataataaat aagaggtcaa tcactgtgtc
    36301 catgggcaat atactccaat gataagaagt cattattgtt tatagtgcgt acaataatgt
    36361 taaggtaaaa cgtcggtcat tataagtttt ttgacatcct ctaaactcat agattttgtt
    36421 aatatttttt caggtatctc ttttaagaag tctttctcaa gccaccacct tgcaagagaa
    36481 tcttctccaa attctaaaga tttaagtcga gttgaatgtc ttttaacagt ttcttgaaag
    36541 cttaaatcat agtaataagc ataagtattg ttggggttat gttttaaaat atgtttccaa
    36601 acaggcgtat accaatcaga acgaagtatg ccttcaagga tgatgtagga acagttatgg
    36661 taaccataat ttagtaaatt gatcaaaagg ggaatggtag tagtattttc accatcctta
    36721 gttcctaaca tctctctacg aaggtaatcc tgagataaga gaagggtatc ttcccctaat
    36781 tcggcttgaa gttgtttagc tattgtagac tttccgctag ctgaatttcc cctgataata
    36841 atgagcgtgg ctaccattag ttcaccatat tgcttttgag ataggcatcc actaagagtt
    36901 cagttgcttg aatagaatcg atatgggtac gttcataaga atgactagat tcaatacctg
    36961 caccaagtag cgcgtgttta acttccgcac cagcacgcat ggcagcggag gcgtcactac
    37021 cataataagg ataaatatca agtttataag gaatattatt gttttcagct agagaaacaa
    37081 gatgttgacg taattcataa tgataagaac cagaagcatc tttaacacag atagagacag
    37141 tatattcgtc agtttcttga tcgtctccca tagctcccat atcaactgct agatattcta
    37201 cagtttcatt tgggatgctt gaatttgctc catgtcctag ctcttcaaag gcactaaagt
    37261 agaaatgagt agtataagga agttgaatgt cttctttctt gtaaacagaa agaagttcca
    37321 ttaggatacc agcggatacc ttgtcatcta ggtaacgtga tttaataaag ccgctgcctg
    37381 ttatgattgt acgcggatca aatgaaatga aatcaccgac ctggatgcct aaagcacgtg
    37441 tttcgtcagc agttgttact ttctcatcta aacgaatttc catatttgtt tgattacgtt
    37501 cagcagttcc agcatctttg taaacatgga cactagtttg atgaataaga gcagttccag
    37561 aaatttcttt accatttttt gagaggtgta tagtacagtt ttctccttca atggcattgt
    37621 atgtgtatcc gccaataagg tcaattttta accgaccatc tggtttgatg gccctaacca
    37681 tagcaccaag tgtatcaagg tgagcagtta tcatgcgatg tttagtatta ttttttcctt
    37741 ttagagaaac catgacgcct cccttgtttg tgcgaattgg tgagtagcca aacgcttcca
    37801 attctttgat gatataggtc atgattgttt gggtaaaccc tgttggtgaa gggattgatg
    37861 tcagggtttt aatgtagttt aatgttgttt ccatactatt tcctttaggc tttaaagttc
    37921 gataatgttt caacgataat atttgcgata cgttcaacgt cgctagaagt tcctcgcaat
    37981 tcaaagtctc ctaacatagg aaatccaaac cgttcagagt attgttttgc agttaaacag
    38041 tactgattgt taaaatttct atttccagag ccaacaattc cgatacaatt tttgacatta
    38101 ttgtgtgcag ctataaaatc accaagtgga atggtaagaa tttcttgatc gccattgtca
    38161 atgccatttc ctccctcgag gtaagtgggt aatagtgcta cgaaaggttg gtcaacagga
    38221 aaggtttgat gattttgttc cttgatatta atagtcgaaa cctgacagtt atgccaagtt
    38281 gacagttgtt cactaagacg tttgacaaag ctttgagtat tgccgctaag actgatatag
    38341 actagggtta atttaggcat ttagtactcc ttaaatctaa atttcttgtt atcagtttag
    38401 caaaatcatg tcagataaac aagctagagt ggtatctcaa tatctgactg tttagagaga
    38461 aaatctattg ttgagactaa aaactgaggt tgattggcct cagtttttag tgatgactat
    38521 atttgtttct ttttaagtaa atttcccacg agtccaagta ttgcgacgcc aaagaaagcc
    38581 agataagatt gtttttcttg tccagtttta ggtaatgttt tttctttctg ttgtgtatag
    38641 atagtgcctg cgatatttgg agaagctttt gcttttgtat aagcagttgc aaccatgtgg
    38701 tgaatttttt gtttctcttt ttggcttaac ttttcttgat gtgatggctc agtaaggtct
    38761 actttttctt gttcagtata gacaaatcga taatcaccaa atccttcatc tttggtagaa
    38821 ctagcgatat actgaatatc cttgctatta gtaacaagat ttttagcttt atcggctgtt
    38881 tggaatcgta aatcaaggcc tttaatagac tctgtgaagc cccagttatt atcagctgta
    38941 ggattgatgg ttttctctga aataatatag tttataatag tttggcgatt ttctaagttc
    39001 agcaaacggt tcattgtggc atttttagcc ccagggaagg taccactagc acggtagtta
    39061 ttggtcacaa tcataaactc ttgtttgtct gcaacaggct taccattgta cattaaatcg
    39121 cgtactcgac tagcttgact attaacaact tttccatctt tgttgtattt attaggttgt
    39181 gtaatatcaa atttataagt caagccatca ataacgtcaa aattataggt acgatattcg
    39241 gtattgataa tattttgcgg tgctttattg ttagggtcaa tttggttaaa ttgaccagcg
    39301 gacatttcta accattcacg taagtctgct ccggtcactt ttaaaagtgc agtaacatta
    39361 tcataaaggt aaagatcagc tacgttttta attgcaacag gtccagctgg aatatcagta
    39421 tatgcggtag catcgccacg tgttccggct ttaaatggtg ctgcagcaga tagaagtggt
    39481 aaatttgcct caggtgttcc ttttaattca tttgcaacgt accaacgttg agcgttgtta
    39541 acaatttgaa ctgaagggtc atctttaact agtgcaaagt aacttgtaat tggcgcagtt
    39601 gttgtaccta cttgttggcg aacatagttg atagtgccat cgtgtgcttc cttagccaat
    39661 gcaatgatac gctcgtcagc agttgttgag ttcatatcaa ttttacggat tttagcactg
    39721 ctttcggaga cttgccattt tccattagta taactaagtc ctaaatcaat aataccaagg
    39781 tgatccccgt acttgcctgc cattgtaaca ggtgttccat ttatttttcc attgatacca
    39841 tcaactccag tgtatttttc atagaagcca gtaccgttac ctgatggaaa ttcagcgtgt
    39901 gagtgtcccg taacaacggc atccactccc ttgatgctgg caatttgata gccaacgttt
    39961 tcttcacctt cttcatatcg atcatctcca atgccagagt gtgataaaac gagaatgaca
    40021 tctgctcctt tagcacgcat agtaggaacg atttcttcga ttgctttaac tgaatctttg
    40081 acaataacct ttccctctaa gttagccttg tcccagttta agatttgagg aggtacaatt
    40141 cctgtaattc caatatttag tgcaacagct cttcctgttg aatctttgaa ggttttagta
    40201 atgatgtcat aagttttaaa aactggttga tgtgttttga aatccaaaac atttgcgttg
    40261 aggattggaa ggttggctgt agcaatgatt tttttgaggt aatctaaacc gtaattaaat
    40321 tcatgattac ctagtgttga tgcatcataa ccaagagcat tcatggcttg gtacatagga
    40381 tgttcttcat tttctgcgac aggtttaacg attgctttat aagttcctaa aggtgtccct
    40441 tgaatgacat ctccgttatc aactaagacg gtattaggat tttctttttt tgcttcttcg
    40501 ataaggacag cagtttttgc taaccctatt ttttgagatt ctttatcttg ataataatca
    40561 tagttaacta aattggtgtg taggtctgtg gttgacatga tgcgaaggtc aactttttca
    40621 ccttcaatag gtttgagatc accagttgtg gtttctgtgg caacaggctg tggattttga
    40681 ttattggcag tggtatttgt gcttgtggct gttgaagcat cagttattgc agttggagta
    40741 gaagtaacgt ttgacttttc ctctgcaatt gtgctagtgg gggtagttga ttcaaccttt
    40801 gtagcggttg aacttggtgt tgtaacaatg tcttctgctt gaacagctaa accacttgtt
    40861 gttgtagctg ttaagacagt taaagcgaat atgctttttg aaaaataatg ttttgacata
    40921 aatctcctcc attaaaacat tacatgtata ttatcctaat ctaaaaacta ttatagctta
    40981 ttgaaaacgt ttaatcaatc tcatttacga attattttta gtaattacac taaaaacaat
    41041 cgtttattta taaaggataa cttggttata cacatttact tggtatttaa ttctttttga
    41101 gcctctaact tcttttattt ttaggttaaa aagtgataaa ataggtacaa tctatcacta
    41161 tgattaaaga ggataactat ggtcaaagaa attaatttaa caggagaaga ggttgttgca
    41221 atcacatccc agtacatgag tgagacggat gttgcttttg taaaatttgc cttaaattac
    41281 gcaaccgcag ctcattatta tcaagcaaga aagtcgggcg agccctacat tattcatcca
    41341 attcaagtag caggtattct tgcagattta caccttgatg ccgttacggt agcttgtggt
    41401 tttttacatg atgttgtaga agatacggaa attacccttg atgagattga aactgatttt
    41461 ggcaaggatg ttcgcgatat tattgacggt gtaacaaaat tgggtaaagt ggagtacaaa
    41521 tctcatgaag aacagttagc agaaaatcac cgtaaaatgt taatggctat gtctaaggat
    41581 atccgtgtta ttttggttaa gctcgctgat cgcttacata atatgagaac ccttaaacac
    41641 ctcagaaaag ataaacaaga gcgtatttca cgtgagacta tggaaatata cgctccttta
    41701 gcgcatcgtt tggggattag tcgtatcaag tgggaattag aagatttatc ttttcgttac
    41761 ttgaatgaga cggaatttta taagatttct catatgatga gtgaaaaacg tcgtgaacgc
    41821 gaagagttgg ttgatataat tgtcgataaa atcagatcct atactgagga acagggttta
    41881 tatggtgata tttatggtag accaaaacac atttattcta tttacagaaa aatgcgtgat
    41941 aaaaagaaac gctttgatca gatttatgat ttaattgcga tacgctgtat tatggaaact
    42001 gctagtgatg tttatgccat ggtggggtat atccatgagt tatggcgtcc gatgccagga
    42061 aggtttaaag attatattgc ggcaccaaag gccaatggtt atcaatctat tcacacgacg
    42121 gtttatggac ctaaagggcc aattgaaatt cagattcgaa ctaaagaaat gcatcaagtt
    42181 gccgagtttg gtgttgcagc ccattgggcc tataaaaaag gaatcaccag taaggttaat
    42241 caagcagagc aatcggttgg tatgggatgg attcaagaat tagttgagct tcaagatgaa
    42301 tcaaaagacg ccaaggattt tgttgactct gttaaggaag atatctttac agaacgcatc
    42361 tatgttttta ccccaaatgg tgctgttcag gaattgccaa gggaatctgg accaattgat
    42421 tttgcttatg ctattcacac gcaagttgga gaaaaagcta ctggcgctaa agtaaatgga
    42481 cgtatggttc cattgactgc taagttaaaa acaggagatg ttgttgagat tatcacaaac
    42541 cctaattcat ttggaccaag tcgtgactgg attaagattg ttaagaccaa taaagctcgc
    42601 aataaaattc gtcagttctt caaaaaccaa gataaagaaa cttctattaa taaaggtaga
    42661 gaattactgg ttgattattt ccaagaacaa ggttatgtgc ctaacaaata tttagataag
    42721 aaacatattg aagaaatcct tccacgtgtc agtgttaaaa gtgaggaagc tttgtatgca
    42781 gccgtaggtt ttggtgattt gagtccgatt agtattttta ataaactgac ggaaaaagaa
    42841 cgccgtgagg aggaacgtgc taaggctaaa gcggaagcag acgaacttat taacggtggc
    42901 gaaatcaaaa ccgataagcg tgatgttctc aaagttaaga gtgaaaatgg tgttatcatt
    42961 caaggagctt caggtttatt aatgcgtatt gcaaaatgtt gtaatcccgt tcctggagat
    43021 ctcattgaag gctatataac taaaggtaga ggcgtggcta tccatcgttc agattgtcaa
    43081 aatttaaaga gtcaggagaa ctacgaacaa cgtttgattg atgttgagtg ggatgatgat
    43141 ggatctaaaa aagagtacat ggctgaaatc gatatttatg gtttgaatcg tagtggcctc
    43201 cttaatgatg ttcttcaaac cttatcaaat gcaacaaagc tagtatcgac agtaaatgca
    43261 caaccaacaa aagatatgaa atttgctaat attcatgtta gctttggaat ttcaaattta
    43321 gcacaattga caactgtggt tgataaaatc aaaattatcc cagatgttta ttctgttaaa
    43381 cgtacgaatg gttaggagag caaaatgaaa atcattattc aacgtgttag tcaagcaagt
    43441 gtttctattg aagatgatgt agtcggatct attgagaagg gtttagtact tctagttggg
    43501 attgctccag aagatacaac agaagatatt gcttatgcag ttcgtaaaat tactagcatg
    43561 cgtatttttt ctgatgatga ggggaaaatg aacctttcaa ttcaagacat taaaggaagc
    43621 gtcttatcta tctcacaatt tacactcttt gcagatacca aaaaaggaaa tcgtccagct
    43681 tttactgggg cagctgatcc tgttaaagcg aatcagtttt atgatatctt taatcaagaa
    43741 ttagcaaatc atgtttcggt agaaacaggt caatttggag ctgatatgca agtcagtctg
    43801 attaatgatg gaccagtaac tatcgtttta gatactaaaa ataaataaaa aatcaaagag
    43861 ttgggcaaaa tgcttggctc tttttattgt ggttctaatt atactcctta acccattcat
    43921 gtttataaaa agattcactg tagcttggaa aggcatgaat aaaggttgtt gatatgaaat
    43981 taagtgcact taacaatatt aaaaatagaa aaatattaga tttatggttt ttcgttgaca
    44041 ataaaataag aatactctaa aatgttaact ggttaagtac tttttatttt aaaggaggtt
    44101 taatgaaaag gatacggaaa agccttattt ttgttctcgg aatagttacc ctaatttgct
    44161 tatgtgcttg tactaaacag agccagcaaa aaaatggctt gtcggtcgtg actagctttt
    44221 atccagtata ttccattaca aaagcagttt ctggtgattt gaatgatatt aaaatgattc
    44281 gatcacagtc aggcattcat ggttttgaac cctcatcaag tgatgttgct gccatttatg
    44341 atgctgatct atttctttat cattcgcaca cactagaagc ttgggcgaga cgtttggaac
    44401 ctagtttgca tcactctaaa gtatctgtaa ttgaagcttc aaaaggtatg actttggata
    44461 aagttcatgg cttagaagat gtagaggcag aaaaaggagt agatgagtca accttgtatg
    44521 accctcacac ttggaatgac cctgtaaaag tatctgagga agcacaactc atcgctacac
    44581 aactagctaa aaaggatcct aaaaacgcta aggtttatca aaaaaatgct gatcaattta
    44641 gtgacaaggc aatggctatt gcagagaagt ataagccaaa atttaaagct gcaaagtcta
    44701 aatactttgt gacttcacat acagcattct catacttagc taagcgatac ggattgactc
    44761 agttaggtat tgcaggtgtc tcaaccgagc aagaacctag tgctaaaaaa ttagccgaaa
    44821 ttcaggagtt tgtgaaaaca tataaggtta agactatttt tgttgaagaa ggagtctcac
    44881 ctaaattagc tcaagcagta gcttcagcta ctcgagttaa aattgcaagt ttaagtcctt
    44941 tagaagcagt tcccaaaaac aataaagatt acttagaaaa tttggaaact aatcttaagg
    45001 tacttgtcaa atcgttaaat caataggtag ttatataaag aaaggacgca ggatgaatcg
    45061 taaaaaaaca gttattatta gtgctttatc agtagctcta tttggtactg gtgtaggtgc
    45121 ttaccaatta ggtagctata atgcccaaaa atcagacaat agtgtttctt atgtgaaaac
    45181 agataaatct gatagcaaag cacaagcaac agctgcaaat aaaacgcccg accaaatcag
    45241 taaagaagaa ggcatatctg cagaacaaat cgttgttaaa attactgatg atggttatgt
    45301 aacttctcat ggagaccatt accattatta caatgggaaa gtgccatatg atgccatcat
    45361 tagtgaagaa ttgattatga aagaccctag ctatgtcttt aataaagctg atgtcattaa
    45421 tgaagtcaaa gatggttata ttatcaaagt taatggtaag tattacctct atcttaaaga
    45481 aggtagcaaa cgaacaaatg ttcgtacaaa ggaacaaatc caaaagcaac gcgaagaatg
    45541 gtctaagggt ggttctaagg gagaatcagg aaagcatagt agtgcaaaaa ctcaagcttt
    45601 atcagccagc gtacgtgaag cgaaagctag cggtcgctat actacagatg atggttatgt
    45661 ttttagtcca acagatgtta ttgatgacat gggagatgcc tttttagtac cacatggcga
    45721 tcatttccat tatatcccga aagctgattt atctccatca gagttatcag ccgcacaggc
    45781 atattggaac cgcaagactg gccgatcagg taatagctca aaaccatcaa attcaagttc
    45841 atacattcat gcaagtgcac caagcggtaa tgtatcaaca ggaagacatg ctaatgcccc
    45901 tatttctatt ccaagagtca ctcacgcaaa tcactggtca aaaccagctg gtaatcatgc
    45961 tacggcacca aaacaccatg cacctacaac caaaccaatc aaaaaagatt ctgctttaga
    46021 caaaatgttg aaacgtttat atgctcaacc gttatctgct cgtcacgtag aaagtgatgg
    46081 tttggtttat gacccagcac aagttaatgc tttcacagca atcggtgttt ctattccgca
    46141 cggtaatcat ttccacttta ttcactataa ggatatgtct ccattagagt tagaagcaac
    46201 aaggatggtg gcagagcata gaggacatca tattgatgca ttagggaaaa aagattctac
    46261 agagaaacca aagcatattt ctcatgaacc taataaggaa cctcacacag aggaagaaca
    46321 ccatgcagta acaccgaaag accaacgtaa aggcaaacca aatagccaga ttgtctacag
    46381 tgctcaagaa attgaagagg caaaaaaagc tggtaaatac acaacatctg atggttacat
    46441 ttttgatgct aaagatatta aaaaagatac aggtacaggt tatgtcattc cacatatgac
    46501 acatgagcat tgggtaccaa agaaagattt atcagagtcg gaattaaaag cagctcaaga
    46561 atttctttca ggaaaatctg aagcaaatca agacaaacca aaaacaggta aaacagctca
    46621 agaaatctat gaggcaattg aaccaaaagc aattgttaaa cctgaagatt tattatttgg
    46681 aattgcacaa gcgacagact ataagaatgg tacatttgta attcctcata aagatcatta
    46741 ccattatgtg gaattaaaat ggtttgatga agaaaaagat cttttagctg attcagataa
    46801 gacatattct ttagaagact atttagctac ggctaaatat tacatgatgc acccagaaaa
    46861 acgtcctaaa gttgaaggat ggggtaaaga tgctgaaatt tataaggaaa aggactctaa
    46921 taaagcagat aaaccaagtc ctgcaccaac tgataataaa tcaacatcaa attctagtga
    46981 caaaaactta agtgctgcag aagtattcaa acaagcaaaa ccagaaaaaa ttgtaccgct
    47041 tgataaaatt gctgctcaca tggcatatgc agttggattt gaagatgatc aattgattgt
    47101 tcctcatcat gatcattatc ataatgttcc tatggcatgg tttgacaagg gtggtttatg
    47161 gaaagcacca gaaggctata cattacaaca actcttctca acaattaaat actacatgga
    47221 acatcctaat gaattaccaa aagaaaaggg ttggggacac gacagtgatc ataacaaagg
    47281 ctcaaataaa gacaataaag ccaaaaatta tgctccagat gaagaacctg aagattcagg
    47341 gaaagtaact cacaactatg gtttttatga tgttaataaa ggttcagacg aagaagaacc
    47401 agaaaaacaa gaagatgaat cagagctaga tgaatatgaa ctaggaatgg cacaaaacgc
    47461 taagaaatat ggtatggata gacaatcttt tgaaaagcaa ctcatccaat tatcaaataa
    47521 atatagtgta agttttgaaa gctttaatta tattaatggt aatcaagttc aagttactaa
    47581 aaaagatggc tctaaagtgc ttgttgatat taaaacacta gcagaagtga aataataaaa
    47641 gagttgaata aacttagctg acaactaaaa acaaacaagt caccgcactt gtttgttttt
    47701 tagttatgat ttctagcgtg cgaagatttt aatgatatat ctaattcttc ttttgaacaa
    47761 ccattgtttt acgcttaaaa gagagttaat gtatttttgt ttgaggaagt tttcttgttt
    47821 tgacttttat aaaacgacta aagttataat cactaaataa agagccacta ctaaaagctg
    47881 tataacaggt ttctagtagt ggctcacttg ttttattaca tgtaattgtt attagttaaa
    47941 attttggtga agctactgta ttgctcctta acaacattat caatattatg atctgttaat
    48001 acaaaatcaa tatcgtctaa ttgataaaaa gtataaaaat catatttacc aaatttttgg
    48061 ctatcaacta aaaggaattt ttcaaaggaa ttattaagag caatgcgctg gatttcacct
    48121 tctccctcgt cataagtagc gatagacttt tcaaaaacac cattactact aacaaaagct
    48181 tttgaaaaat taagtgattt aatagagtta atagtaacag aaccgacaaa agcaccagta
    48241 atttctctgt actctcctcc tacaaggata aggtcaagtg ttgaactttg attcagaata
    48301 ttgaaaacag gaaggctatt ggttactacc ctaattttaa aatcaagtaa ttgcgtagct
    48361 aatttctcta aggttgttcc aggcccaata aagattgttt caccatcatt gatgaattgt
    48421 tttgcaaatt gggcaatttc taatttttca tttgtttgaa tatcgtattt ttcagtgttt
    48481 gatttttcat aattttgtgg aattggtgct gcgtttactt tttgtgcacc accatggatg
    48541 cgtattaaca gtcctgcttt atctaattca tccaagtcac gcctaacagt catgtctgat
    48601 acatcaagtt cttccatgat ttcattaaca gtgacaatac catttatatt gactttttct
    48661 attatttttt gtaatctttc tcgctttaac ataataatct ctcctacctc ttcattgtat
    48721 atatcatacc ataaatagag ctaaaataat aataattttg aaaaaaacaa acaataataa
    48781 acaaaaataa ataaaaaacg tcttgtgatg ttttattttg ttttgtataa tattgatgta
    48841 gataa
//
LOCUS       DATROQ010000004        46535 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.004, whole genome shotgun sequence.
ACCESSION   DATROQ010000004 DATROQ010000000
VERSION     DATROQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46535
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.004"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..305)
                     /gene="dhaS"
                     /locus_tag="VBC94_000166"
     CDS             complement(<1..305)
                     /gene="dhaS"
                     /locus_tag="VBC94_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEO6978472.1"
                     /translation="MTSSLITKKKIAKSFKRLFISQAFDKISVSDIMEDAGIRRQTFY
                     NHFVDKYALLEWIFQTELSEQVTDNLDYISGFQLLSELLTFFKMNQEFYIKLFQIED"
     gene            479..1468
                     /gene="dhaK"
                     /locus_tag="VBC94_000167"
     CDS             479..1468
                     /gene="dhaK"
                     /locus_tag="VBC94_000167"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEO6978473.1"
                     /translation="MKKILNQPTDVVTEMLDGLAYVHNDLVHRIEGFDIIARNEEKSG
                     KVALISGGGSGHEPSHAGFVGEGMLSAAVCGAVFTSPTPDQVLEAIKEADEGAGVFMV
                     IKNYSGDIMNFEMAQDMAEMEGIEVASVVVDDDIAVEDSLYTQGKRGVAGTILVHKIL
                     GHAARHGKSLQEIKAIADELVPNIHTVGLALSGATVPEVGKPGFVLAEDEIEFGIGIH
                     GEPGYRKEKMQPSKALATELVDKLIESFDAKSGEKYGVLINGMGATPLMEQYVFANDV
                     AKLLEDKGIEVNYKKLGNYMTSIDMAGLSLTLIKLENQEWLEALNSDVTTIAW"
     gene            1506..2084
                     /gene="dhaL"
                     /locus_tag="VBC94_000168"
     CDS             1506..2084
                     /gene="dhaL"
                     /locus_tag="VBC94_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEO6978474.1"
                     /translation="MEVKTAIEWMHTFNQKIQSNKDYLSELDTPIGDGDHGGNMARGM
                     TAVIENLDNNEFSSAADVFKTVSMQLLSKVGGASGPLYGSAFMGITKAEQSESTISEA
                     LGAGLEMIQKRGKAELNEKTMVDVWHGVIEAIEKNELTEDRIDSLVDATKGMKATKGR
                     ASYVGERSVGHIDPGSFSSGLLFKALLEVGGV"
     gene            2084..2458
                     /locus_tag="VBC94_000169"
     CDS             2084..2458
                     /locus_tag="VBC94_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEO6978475.1"
                     /translation="MSDIGIIVVSHSKNIAQGVVDLISEVAKDVSITYVGGTEDGEIG
                     TSFDQVQQIVEQNDKKTLLAFFDLGSAKMNLELVADFSEKNIIINSVPVVEGAYTAAA
                     LLQAGADLDSIQSQLAELTINK"
     gene            2468..3181
                     /locus_tag="VBC94_000170"
     CDS             2468..3181
                     /locus_tag="VBC94_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6978476.1"
                     /translation="MTQFLGEFLGTFILVLLGDGVVAGNVLSKTKEEGTGWTAIVFGW
                     GIACTVAVYVSGLFSPAHLNPAVTLAMASIGVISWGQVIPFIIAQMLGAMVAATILWL
                     HYYPHWKETKDSGLILASFSTGPAIRHTPSNLLGEIIGTAILVITIMAIGPSKVAAGL
                     DPIIVGIVIFAVGFSLGPTTGYAINPARDLGPRLMHAILPIENKGNSDWSYAWIPVVG
                     PIIGGVLGAILYNIALTLV"
     gene            complement(3446..3850)
                     /locus_tag="VBC94_000171"
     CDS             complement(3446..3850)
                     /locus_tag="VBC94_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO6978477.1"
                     /translation="MKGFHGNIEKLTLGNTNFRQVLYTAEHCQLVLMTLPVGGEIGSE
                     IHAENDQFFRFEAGHGKVVIDGNEYEVADGDAIIVPAGAEHNVINTSETEMLKLYTIY
                     SPAHHKDGIIRATREEAEENEEDFDGVTTETK"
     gene            complement(3989..4703)
                     /locus_tag="VBC94_000172"
                     /pseudo
     CDS             complement(3989..4703)
                     /locus_tag="VBC94_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859560.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(4796..5905)
                     /locus_tag="VBC94_000173"
     CDS             complement(4796..5905)
                     /locus_tag="VBC94_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEO6978478.1"
                     /translation="MTVTGIVAEFNPFHNGHKYLLEQAQGIKVIAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVIELPFLVSVQSADYFASGAVSILARLGVDNLCFGTEEMLD
                     YARIGDIYVNKKEEMEAFLKKQSDSLSYPQKMQAMWQGFAGITFSGQTPNHILGLAYT
                     KAASQNGIRLNPIQRQGAGYHSSEKTEIFASATSLRKHQSDRFFVEKGMPNSDLFLNS
                     PQVVWQDYFSLLKYQIMTHSDLTQIYQVNEEIANRIKSQIRYVETVDELVDKVATKRY
                     TKARIRRLLTYILINAVESPIPNAIHVLGFTQKGQQHLKSVKKSVDIVTRIGSQTWDS
                     LTQRADSVYQMGNANIAEQTWGRIPFYQPVSQSDL"
     gene            complement(5902..6153)
                     /locus_tag="VBC94_000174"
     CDS             complement(5902..6153)
                     /locus_tag="VBC94_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978479.1"
                     /translation="MIIFLYACLMIFGILYIIERRVSQKKSKVILELLMNPKLHLDLL
                     SALKPFEKETDRLIYLRKTYHLDIIDAVTLNNQLKEKEV"
     gene            complement(6176..6910)
                     /locus_tag="VBC94_000175"
     CDS             complement(6176..6910)
                     /locus_tag="VBC94_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6978480.1"
                     /translation="MATYETFAAVYDAVMDDTLYDKWTDFSLRHFPKGKKKLLELACG
                     TGIQSVRFAQAGYAVTGLDLSGDMLKLAKKRATSAHQSIQFIEGNMLDLSNVGKYDLI
                     TCYSDSICYMQDEVEVGDVFIEVYKALEENGVFIFDVHSTYQTDKVFPGYSYHENADD
                     FAMVWDTYEDDAPHSIVHELTFFVQEEDGRFTRHDEVHEERTYDILTYDILLEQAGFK
                     DVKVYADFEDKKPTATSARWFFVAHK"
     gene            complement(6976..7332)
                     /gene="rsfS"
                     /locus_tag="VBC94_000176"
     CDS             complement(6976..7332)
                     /gene="rsfS"
                     /locus_tag="VBC94_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEO6978481.1"
                     /translation="MTEKDLLQLVVKAADEKRAEDIVILDLQPVTSVADYFVIMSASN
                     SRQLEAIADNIREQVKGNGGDASHLEGDSKAGWVLLDLNSVVVHIFSEDERQHYNLEK
                     LWHEAPLLDAEVFMTE"
     gene            complement(7334..7855)
                     /locus_tag="VBC94_000177"
     CDS             complement(7334..7855)
                     /locus_tag="VBC94_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO6978482.1"
                     /translation="MTCALLLIDIQQGIMDKKPKHLTNFAELLDDLLLSAKGSNCEVI
                     WIRHHDKELPQGSPQWEIWEQRHLVTHHKIIDKTYNSCFKDTHLHDYLQSKHISQLIM
                     MGLQTEYCFDTSVKVAFEYGYDIFIPQGGHLTFDTPTLSGDSIKKHYENIWHHRFATM
                     VAKDSLLKLINEE"
     gene            complement(7918..8505)
                     /gene="yqeK"
                     /locus_tag="VBC94_000178"
     CDS             complement(7918..8505)
                     /gene="yqeK"
                     /locus_tag="VBC94_000178"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEO6978483.1"
                     /translation="MTYKDYTGLDRTELLSKVRHMMSDKRFNHVLGVERAAIELAERY
                     GYDKEKAGLAALLHDYAKELSDDEFLRLIDKYQLDPDLKKWGNNIWHGLVGIYKIQED
                     LAIKDQDILAAIAKHTVGSAQMSTLDKIVYVADYIEHNRDFPGVEEARELAKVDLNKA
                     VAYETARTVAFLASKAQPIYPKTIETYNAYIPYLD"
     gene            complement(8502..9134)
                     /locus_tag="VBC94_000179"
     CDS             complement(8502..9134)
                     /locus_tag="VBC94_000179"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEO6978484.1"
                     /translation="MALELLTPFTKVELEEKKRDTNRKQIGIMGGNFNPVHNAHLVVA
                     DQVRQQLCLDQVLLMPEFQPPHIDKKETIDEQHRLKMLELAIEGIDGLSIEPIEIERK
                     CISYTYDTMKLLIEKNPDVDYYFIIGADMVEYLPKWHRIDELVKMVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRQFIKSNRQPNYLLPREVLDYIRKEGLYK"
     gene            complement(9264..9581)
                     /gene="yhbY"
                     /locus_tag="VBC94_000180"
     CDS             complement(9264..9581)
                     /gene="yhbY"
                     /locus_tag="VBC94_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEO6978485.1"
                     /translation="MLTSKQRAFLKSEAHSMKPIIQIGKNGLNDQIKTSVRNALDARE
                     LIKVTLLQNTDEDIHDVAEVLEDEIGCDTVLKIGRILILYKESARKENRKISVKVKAV
                     KSN"
     gene            complement(9674..10792)
                     /gene="yqeH"
                     /locus_tag="VBC94_000181"
     CDS             complement(9674..10792)
                     /gene="yqeH"
                     /locus_tag="VBC94_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEO6978486.1"
                     /translation="MEELFCIGCGARIQTENKDAAGYTPRAALEKGLETGELYCQRCF
                     RLRHYNEITDVHITDDEFLKLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFVAGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPVDVILTSAQNHHAIKDLIDTIEKY
                     RHGRDVYVVGVTNVGKSTLINAIIREITGSRDVITTSRFPGTTLDKIEIPLDDGSYIF
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNSEQTLFLAGLARFDFISGQKQ
                     GFTAYFDNNLNLHRTKLVGADEFYTKHVGKLLTPPTGKEVSDFPKLVRHEFTIKDKMD
                     IVYSGLGWIRVKSEAENPVVVAAWAPEGVAVVLRKALI"
     gene            complement(10792..11352)
                     /locus_tag="VBC94_000182"
     CDS             complement(10792..11352)
                     /locus_tag="VBC94_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEO6978487.1"
                     /translation="MNSKIYFLEMKLSIDDYRPTFVVEAVYDLRAEDLLRHGIRAVLV
                     DLDNTLIAWNNPDGTAEVRAWLDEMTTADISVVVVSNNNHARVERAVSRFGVDFVSRA
                     MKPFTRGINMAIERYGFDRDEVIMVGDQLMTDIRASYRAGIKSVLVKPIVKSDAWNTK
                     FNRLRERRVWKKIEENYGKIVYQKGI"
     gene            complement(11436..12350)
                     /locus_tag="VBC94_000183"
     CDS             complement(11436..12350)
                     /locus_tag="VBC94_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEO6978488.1"
                     /translation="MTKKEKGTMMTLAAGLAWGISGISGQYLMSHGVHVNLLTSLRLL
                     ITGIFLLSLARSKQKEHLVAAWKQPKFLKQVLLFSIFGLVLNQYAFLRAIHLTNAGTA
                     TVLQYMAPILILSIVCILNRQRPTSFEIIAIAMAILGTYMIATHGKLGSLAITPKGLM
                     WGLGSAITYSIYILLPVKLIHEWGSTIVIGSGMFIGGILFSLVTKAWQYPLQINVMSI
                     LAYIGIIGIGTIFAYTFFLKGVSIVGAVKGSLLASVEPVSSVFLTVLVLGEIFYPIDL
                     LGMLFIFLAVTLISYKDLIALKKMKRVR"
     gene            complement(12480..13922)
                     /gene="gatB"
                     /locus_tag="VBC94_000184"
     CDS             complement(12480..13922)
                     /gene="gatB"
                     /locus_tag="VBC94_000184"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEO6978489.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPSSAHFGQEQNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHQNMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIELEDGTRKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEEFGTKAELKNLNS
                     FNNVRKGLIHEEKRQAQVLRSGGQIQQETRRFDETTGETILMRVKEGSSDYRYFPEPD
                     LPLFDISDEWIDQVRLELPEFPQERRAKYVSSFGLSSYDASQLTATKATSDFFEKAVA
                     IGGDAKQVSNWLQGEVAQFLNSESKSIEEIGLTPENLVEMIGLIADGTISSKIAKKVF
                     VHLAKNGGSAEEFVKKAGLVQISDPEVLIPIIHQVFADNEAAVIDFKSGKRNADKAFT
                     GYLMKATKGQANPQVALKLLAQELAKLKEE"
     gene            complement(13922..15388)
                     /gene="gatA"
                     /locus_tag="VBC94_000185"
     CDS             complement(13922..15388)
                     /gene="gatA"
                     /locus_tag="VBC94_000185"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEO6978490.1"
                     /translation="MSFNNQSIDQLHDFLVKKEISATELTKATLEDIHAREQAVGSFI
                     TISDEMAIAQAKEIDDKGIDADNVMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATAVEKLYAKDMIVIGKANMDEFAMGGSTETSYFKKTNNAWDHSKVPGGSSGGSAAAV
                     ASGQVRLSLGSDTGGSIRQPASFNGIVGMKPTYGRVSRFGLFAFGSSLDQIGPMSQTV
                     KENAQLLTVISGHDVRDSTSSERTVGDFTAKIGQDIQGMKIALPKEYLGEGIAQGVKE
                     TIIKAAKHLEKLGAVIEEVSLPHSKYGVAVYYIVASSEASSNLQRFDGIRYGYRTENY
                     KNLDDIYVNTRSEGFGDEVKRRIMLGTFSLSSGYYDAYYKKAGQVRSLIIQDFEKVFA
                     DYDLILGPTAPTTAFDLDSLNHDPVAMYLADILTIPVNLAGLPGISIPAGFDQGLPVG
                     MQLIGPKFSEETIYQVAAAFEATTDYHKQQPKIFGGEN"
     gene            complement(15388..15690)
                     /gene="gatC"
                     /locus_tag="VBC94_000186"
     CDS             complement(15388..15690)
                     /gene="gatC"
                     /locus_tag="VBC94_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEO6978491.1"
                     /translation="MKISEEEVRHVAHLSKLRFSDQETKEFASSLSKIVDMIELLNEV
                     DTEGVPVTTTMADRKTVMREDIAQPGHNRDDLFKNVPQHQDYYIKVPAILEDGGDA"
     gene            complement(15828..18473)
                     /locus_tag="VBC94_000187"
     CDS             complement(15828..18473)
                     /locus_tag="VBC94_000187"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEO6978492.1"
                     /translation="METKFVYHFDEGCKEMKELLGGKGANLAEMTSIGLPVPQGFTIT
                     TQACNDYYDNACHIRESILSQIDQAMAQLEVEQNKQLGSVDDPLLVSVRSGSVFSMPG
                     MMDTVLNLGLNDRSVQGLVKKTEDERFAYDSYRRFIQMFADVVTGIPKYKFDTILDRL
                     KTDKCYQDDTELTGSDLKRLVEFYKELYQKEAGEKFPQDPKRQLLLAIEAVFKSWNNP
                     RAKIYRKLNDIPETLGTAVNIQAMVFGNMGNNSGTGVAFTRNPSTGAANLFGEYLINA
                     QGEDVVAGIRTPQSISKLAEQMPIIYQEFVSVTQKLEAHYRDMQDMEFTIENGNLYML
                     QTRSGKRTAKAAIKIAVDQVNEGLISKEEAILRIEPKQLDQLLHPSFDLKSLKKAIIL
                     TTGLPASPGAAYGKVYFHAEDVVKEMKKGNPVLLVRQETSPEDIEGMVSANGIITARG
                     GMTSHAAVVARGMGKPCVAGCSQLLVDEVRREISIGHQTIKEGEMLSIDGATGNVYIG
                     QVPMAETSVDRDFEIFMKWVDENRDMMVCSNADNPRDAQKALDFGAEGIGLCRTEHMF
                     FDDERIPVVREMILADEILSRRKALERLLSFQRDDFYQIFKVLKGKACTIRLLDPPLH
                     EFLPHDKESIESMARQMGISTLAIEKRIQTLEEFNPMLGHRGCRLAITYPEIYQMQVR
                     ALVQGAILAMKEGYEAKPEIMIPLVTAHEEISIIRDLIEETIVEESKSKKINLSFPIG
                     TMIETPRACMIADDIAKFADFFSFGTNDLTQMSFGFSRDDAGKFLGEYVDKGLLKKDP
                     FQVLDQKGIGRFIEQAVRLGKEVKPNLKIGICGEHGGEPSSIEFCYQQGLHYVSCSPF
                     RIPIAKLAAAQAKIKQSRNDITIDK"
     gene            complement(18486..19316)
                     /locus_tag="VBC94_000188"
     CDS             complement(18486..19316)
                     /locus_tag="VBC94_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEO6978493.1"
                     /translation="MEDQLTIFIISDSLGETAKAIAKACLSQFPGHDDWHFQRFSYIN
                     SQERLEQVFEEASQKTVFMMFSLVDVALASYAQKRCESEHYAYVDLLTNVIQGISRIS
                     GIDPLGEPGILRRLDNDYFKRVESIEFAVKYDDGRDPRGILQADLVIIGISRTSKTPL
                     SMFLADKNIKVINIPLVPEVPVPKELRMIDSRRIIGLTNSVDHLNQVRKVRLKSLGLS
                     STANYASLERILEETRYAEEVMKNLGCPIINVSDKAIEETATIILEILKTNGQVAKNL
                     "
     gene            complement(19327..19956)
                     /locus_tag="VBC94_000189"
     CDS             complement(19327..19956)
                     /locus_tag="VBC94_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6978494.1"
                     /translation="MQLNQRQERIIDIIKQFQPITGELIAEKLGVTRAALRSDLAVLS
                     MLGIIDAKPKVGYFYLGQYHASIGTSHFEKMTVSEIMGIPLTVHQKDSVYDVIVHIFM
                     EDAGCAFILDDDDFLCGVVSRKDLLKTSIGGGDLSKMPIGMVMTRMPHVTTVLENESL
                     FAAADKLVSRKVDSLPVVCHDKQYPEKFKVIGKLSKTILASLFLEIRDY"
     gene            20145..21110
                     /locus_tag="VBC94_000190"
     CDS             20145..21110
                     /locus_tag="VBC94_000190"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEO6978495.1"
                     /translation="MTIKNLTVAGSGVLGSQIAFQAAYKGMSVTIYDINDEALNKGKE
                     RIKKLAKVYQSEIETAKEAYSDKAKSIKYNKNLLPSLDHIFLSKVADSLDLIADLPNQ
                     ITFSKNLDQAVSDADLVIEAVPETVSIKEDFYKQLAKVAPSKTIFATNSSTLVPSQFA
                     DITGRPDKFLAMHFANNIWQNNIVEIMGHKGTDDEVVKEALAFSKDIGMVPLYIHKEQ
                     PGYILNSILVPFLESALALYYNKVSDSETIDKTWKLGTGAPMGPLEILDIIGIDTAYN
                     IMKNYSDTTSDPNSLHAHLAKMLKEEFIDKGRTGKAAGHGFYDYD"
     gene            complement(21157..21705)
                     /locus_tag="VBC94_000191"
     CDS             complement(21157..21705)
                     /locus_tag="VBC94_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO6978496.1"
                     /translation="MTKALISIDYTYDFVADDGKLTAGKPAQSIASAIADVTEKAYRS
                     GDYIFFAIDNHDIGDVFHPETNLFPEHNIKGTSGRNLYGPLGTLYETIKEDSRVFWID
                     KRHYSAFSGTDLDIRLRERRVDTLILTGVLTDICVLHTAIDAYNLGYKIEVPAAAVAS
                     LNDSNHQWALNHFKTVLGATIL"
     gene            complement(21772..22557)
                     /gene="codY"
                     /locus_tag="VBC94_000192"
     CDS             complement(21772..22557)
                     /gene="codY"
                     /locus_tag="VBC94_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEO6978497.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLDAELPYNTMAAQLADIIDCNAC
                     IINGGGNLLGYAMKYKTNTDRVEEFFETKQFPDYYVKSASRVYDTEANLSVDNDLSIF
                     PVETKENFQDGITTIAPIYGGGMRLGTFIIWRNDKEFSDDDLILVEIASTVVGIQLLY
                     LQTENLEENIRKQTAVTMAINTLSYSEMKAVAAILGELDGLEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFDKLKEYN"
     gene            complement(22683..23894)
                     /locus_tag="VBC94_000193"
     CDS             complement(22683..23894)
                     /locus_tag="VBC94_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6978498.1"
                     /translation="MKIFDKSMKLEHVAYDIRGPVLEEADRMRANGEKILRLNTGNPA
                     AFGFEAPDEVIRDLITNARESEGYSDSKGIFSARKAVMQYYQLQNIHVDMDDIYIVNG
                     VSEGISMSMQALLDNDDEVLVPMPDYPLWTACVSLAGGNAVHYICDEEANWYPDIDDI
                     KSKITSKTKAIVLINPNNPTGAVYPREILQEIVDIARQNDLIIFSDEVYDRLVMDGME
                     HIPIASIAEDIFTVTLSGLSKSHRICGFRVGWMVLSGPRQHVKGYIEGLNMLANMRLC
                     SNVLAQQVIQTSLGGQQSIDSMLLPGGRIYEQRNYIHKAINEIPGLSAVKPNAGLYLF
                     PKIDTDMYRIDNDEEFVLNFLKQEKVLLTHGRGFNMNTADHFRIVYLPRVDELTELQE
                     KMARFLSQYKR"
     gene            24161..24613
                     /locus_tag="VBC94_000194"
     CDS             24161..24613
                     /locus_tag="VBC94_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO6978499.1"
                     /translation="MSQKYERILVAIDGSTESELAFEKAVNVALRNDSELILTHVIDT
                     RALQSFATFDTYIYEKLEKEAKDVLEEYEKQAREKGADKVRQVIEFGNPKTLLAHDIP
                     EKEKVDLIMVGATGLNTFERFLIGSSSEYILRHAKVDLLIVRDPNKTM"
     gene            complement(24668..26050)
                     /locus_tag="VBC94_000195"
     CDS             complement(24668..26050)
                     /locus_tag="VBC94_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO6978500.1"
                     /translation="MAIKAVFFDIDGTLLNDRKNVQKSTIKAIRNLKDQGILVGLATG
                     RGPSFVQPFLENLGLDFAVTYNGQYIYSRSEIIYTNQLSKTTVYRLIRYAGARRREIS
                     LGTASGLLGSGIIGLGTSRLGQIVSSLVPRKWAKAIERSFKHFIRRIKPQNIDSLMVI
                     LREPIYQVVLVATEGESERIQKQFPRVKLTRSSPYSMDVISEGQSKVKGIERVGQCYG
                     FDLSEVIAFGDSDNDIEMLSQVGIGVAMGNASQQVKENARYTTADNNDDGISKALAHY
                     GLIQFEIEKTFSSRDENFNKVKSFHLLMDGETIKTPRLYDSKEAGFRSDFKVEEIVEF
                     LYAASQGNQEVFDQSIRNLHLAIDKARDKVISKDHPETPLVGEVDALTDLLYLTYGSF
                     VLMGVDPKPLFDTVHEANMGKIFPDGKAHFDPVTHKILKPDDWEEHFAPEPSIRRELD
                     SQIQKSLNRK"
     gene            26119..27081
                     /locus_tag="VBC94_000196"
     CDS             26119..27081
                     /locus_tag="VBC94_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEO6978501.1"
                     /translation="MKKILVLHTGGTISMNANEKGQVMSSADNPMKYVDLSLDDLDLT
                     VVDFLNLPSPQITPHHMLDIYHYLKQHASNFDGVVITHGTDTLEETAYFLDTMILPKI
                     PIIITGAMRSTNELGSDGVYNYLSALRVANSTKAADKGVLVVMNDEIHAAKYVTKTHT
                     TNVSTFQTPTHGPLGIIMKQDLLFFKATEERVRFDLDKITGTVPIVKAYAGMGDSGII
                     SFLNSQNISGLVIEALGAGNMPPKAAQEIEELIEQGVPVVLVSRCFNGIAEPVYGYEG
                     GGAKLQESGVMFVKELNAPKARLKLLIALNAGLTGQNLKDYIEG"
     gene            complement(27116..27994)
                     /gene="aroE"
                     /locus_tag="VBC94_000197"
     CDS             complement(27116..27994)
                     /gene="aroE"
                     /locus_tag="VBC94_000197"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEO6978502.1"
                     /translation="MNSLNGETLLTGLIANPARHSLSPLMWNTSFQEKNMNYAYLTFE
                     VEEGKLTEAVRGVRALSIRGVNVSMPFKQSVIPLLDDLSPQAKLVGAVNTIVNQGGTG
                     RLVGHMTDGIGCFKALAAQGFSAKNKIITIAGIGGSGKAVAVQAAMEGVAEIRLFNRN
                     SSNYDKVIDLSDKIKKQFQIKVVVDYLENKTAFKDAIRTSHFYIDATSLGMRPLDNYS
                     LINDPEILTPNLVVVDLVYKPKETALLRFVRQNGVKHAYNGLGMLIYQGAEAFQLITN
                     QEMPISSVERVLQTEN"
     gene            complement(28131..29006)
                     /locus_tag="VBC94_000198"
     CDS             complement(28131..29006)
                     /locus_tag="VBC94_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6978503.1"
                     /translation="MLQNIGQTGIQATRIALGCMRMSDLKGKQAEEVVGTALDLGINF
                     FDHADIYGGGLSELRFRDAIKHLNVNRDKMIIQSKCGIREGYFDFSKEYILSSVDGIL
                     ERLGTEYLDFLILHRPDVLVEPEEVAEAFTKLRAEGKVKHFGVSNQNRFQMELLQSYL
                     DEPLAVNQLQLSPAHTPMFDAGLNVNMLNKASIEHDDGIVDYCRLKRVTIQAWSPFQI
                     DLSRGLFVNHPDYKELNETIAKLAKNYNVSSEAIVIAWILRHPAKMQAIVGSMNPSRL
                     KAIDKANDIALTRKE"
     gene            complement(29297..31312)
                     /gene="recG"
                     /locus_tag="VBC94_000199"
     CDS             complement(29297..31312)
                     /gene="recG"
                     /locus_tag="VBC94_000199"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEO6978504.1"
                     /translation="MLLQSPISNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVSQGISQSTLIKAIKSAFE
                     INAHLELKENLPATLLGKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENKSETNGLSILYSKHAMETKISSLPFILTNAQKRSLDEILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSIIDELPAGRKPIITRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     YKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARRVASDIVKDNNWKENTEWALILDNLRQHSDFD"
     gene            complement(31392..32930)
                     /locus_tag="VBC94_000200"
     CDS             complement(31392..32930)
                     /locus_tag="VBC94_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6978505.1"
                     /translation="MSKQKVTATLLLSTLVLSLSSPLVTLAETINPETSLTMATASTE
                     SSSEAEKQEKTQPTDSETASPSAEGSISTEKTEIGTTETSSSNESSSSSSHQSSSNED
                     AKTSDSASTASTPSTNTTNSSQADSKPGQSTKTELKPEPTLPLVEPKITPAPSQIESV
                     QTNQNASVPALSFDDNLLSTPISPVTATPFYVEHWSGQDAYSHYLLSHRYGIKAEQLD
                     GYLKSLGIQYDSNRINGAKLLQWEKDSGLDVRAIVAIAVLESSLGTQGVAKMPGANMF
                     GYGAFDHDSSHASAYNDEEAIMLLTKNTIIKNNNSSFEIQDLKAQKLSSGQLNTVTEG
                     GVYYTDNSGTGKRRAQIMEDLDRWIDQHGGTPEIPAALKALSTASLADLPSGFSLSTA
                     VNTASYIASTYPWGECTWYVFNRAKELGYTFDPFMGNGGDWQHKAGFETTHSPKVGYA
                     VSFSPGQAGADGTYGHVAIVEEVKKDGSVLISESNAMGRGIVSYRTFSSAQAAQLTYV
                     IGHK"
     gene            complement(33023..34123)
                     /locus_tag="VBC94_000201"
     CDS             complement(33023..34123)
                     /locus_tag="VBC94_000201"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO6978506.1"
                     /translation="MISSYHRPTRALIDLEAIANNVKSVQEHIPSDKKTFAVVKANAY
                     GHGAVEVSKYIESIVDGFCVSNLDEAIELRQAGIVKMILVLGVVMPEQVILAKNENIT
                     LTVASLEWLRLCQTSAVDLSGLEVHIKVDSGMGRIGVRQLDEGNKLISELGESGASVK
                     GIFTHFATADEADNCKFNQQLTFFKDFISGLDNCPDLVHASNSATSLWHSETIFNAVR
                     LGVVMYGLNPSGTDLDLPYPINPALSLESELVHVKQLHDGSQVGYGATYQVTGDEFVG
                     TVPIGYADGWTRDMQGFSVIVNGELCEIIGRVSMDQMTIRLPQKYTIGTKVTLIGQQG
                     SCNITTTDVAQKRQTINYEVLCLLSDRIPRYY"
     gene            complement(34120..34479)
                     /locus_tag="VBC94_000202"
     CDS             complement(34120..34479)
                     /locus_tag="VBC94_000202"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEO6978507.1"
                     /translation="MIVGHGIDLQEIEAITKAYERNQRFAERVLTEQELLLFKGISNP
                     KRQMSFLTGRWAAKEAYSKALGTGIGKVNFHDIEILSDDKGAPLITKEPFNGKSFVSI
                     SHSGNYAQASVILEEEK"
     gene            complement(34504..35511)
                     /locus_tag="VBC94_000203"
     CDS             complement(34504..35511)
                     /locus_tag="VBC94_000203"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEO6978508.1"
                     /translation="MGFHQLSDKINIEILKQKTSLDLEVSQKKLAKEEELKNIIKGED
                     QRFLVIVGPCSADNPKAVLTYAKRLAKLEAAFKDKMFLVMRVYTAKPRTNGDGYKGLV
                     HHSDKLGVFFQARKMHYDIIRETGLLTADELLYPEMLSVMDDLVSYYAIGARSVEDQG
                     HRFISSGIDAPVGMKNPTSGNLRVMFNAVYAAQNQQELFYQNKQVRTDGNLLSHVILR
                     GYHNADYRSIPNYHYENLLETITHYEETDLQNPFIVVDTNHDNSGKQFLEQIRIVKSV
                     LADRQWHTKIRNYVRGFLIESYLEDGRQDKPDVFGKSITDPCLGWDKTEMLIRDIYNS
                     L"
     gene            complement(35637..38165)
                     /gene="secA"
                     /locus_tag="VBC94_000204"
     CDS             complement(35637..38165)
                     /gene="secA"
                     /locus_tag="VBC94_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEO6978509.1"
                     /translation="MANILRTVIENDKGELKKLDKIAKKVDSYADHMATLSDEALQAK
                     TPEFKERYQNGETLDQLLPEAFAVVREASKRVLGLYPYHVQIMGGIVLHHGDIPEMRT
                     GEGKTLTATMPVYLNAISGLGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PFEKREAYNCDITYSTNAEVGFDYLRDNMVVRQEDMVQRPLNYALVDEVDSVLIDEAR
                     TPLIVSGPVSSEMNQLYTRADMFVKTLNSDDYIIDVPTKTIGLSDTGIDKAENYFHLN
                     NLYDLENVALTHYIDNALRANYIMLLNIDYVVSEEQEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKESVPIQEESKTSASITYQNMFRMYHKLAGMTGTGKTEEEEFREIYNMRVIP
                     IPTNRPVQRIDHSDLLYPTLDSKFRAVVADVKERYEQGQPVLVGTVAVETSDLISRKL
                     VAAGVPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGTDRIKVVLERMNLAE
                     DDTVIKSKMLTRQVESAQRRVEGNNYDTRKQVLQYDDVMREQREIIYANRREVITAER
                     DLGSELKGMIKRTIKRAVDAHSRSDKNTAAEAIVNFARSALLDEEAITVSELRGLKEA
                     EIKELLYERALAVYEQQIAKLKDPEAIIEFQKVLILMVVDNQWTEHIDALDQLRNSVG
                     LRGYAQNNPIVEYQSEGFRMFQDMIGSIEFDVTRTLMKAQIHEQERERASQHATTTAE
                     QNISAQHVPMNNESPEYQGIKRNDKCPCGSGMKFKNCHGLRCLQ"
     gene            complement(38274..39221)
                     /gene="manA"
                     /locus_tag="VBC94_000205"
     CDS             complement(38274..39221)
                     /gene="manA"
                     /locus_tag="VBC94_000205"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEO6978510.1"
                     /translation="MSEPLFLEASMHDKIWGGTKLRDEFGYDIPSETTGEYWAISAHP
                     NGVSRVKNGRFKGCFLDKLYQGEKSLFGNPDDTVFPLLTKILDANDWLSVQVHPDDAY
                     ALKHEGELGKTECWYIISADEGSEIIYGHNAKTKEELRQMIESGDWEHLLTRIPVKSG
                     DFYYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRPDASGKLRDLRIEQSIDVLTI
                     GKPANTVPANMKLKHLSSTLLVSNDFFTVYKWEISGVTNFKQFAPYLLVSVLDGAGHI
                     TVDNKVYTLKKGDHFILPNDVVKWDIDGQLEIIASHVKK"
     gene            complement(39339..40220)
                     /locus_tag="VBC94_000206"
     CDS             complement(39339..40220)
                     /locus_tag="VBC94_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO6978511.1"
                     /translation="MTKLYGSIEAGGTKFVCAVGDEELKVVEKMQFPTTTPQETIKKT
                     VDFFKRFEKKLEAVAIGSFGPIDIDKKSKTYGYITTTPKLHWANVDLLGLISKDFNVP
                     FYFTTDVNSSAYGEVIARNNIDSLVYYTIGTGIGAGAIQKGEFIGGTGHTEAGHTYMA
                     MHPQDQANDFKGICPFHNSCLEGLASGPTLEARTGIRGELIEENSMVWDVQAYYIAQA
                     AIQATVLYRPQVIVFGGGVMAQEHMLRRVRQTFATLLNGYLPVPDLSDYIVTPAIEEN
                     GSATLGNFALAKKISKG"
     gene            complement(40288..42207)
                     /locus_tag="VBC94_000207"
     CDS             complement(40288..42207)
                     /locus_tag="VBC94_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO6978512.1"
                     /translation="MASNTEIAKQVINAIGGASNVRSVAHCATRLRIMVKDETVIDKN
                     TVENIEKVQGAFFNSGQYQIIFGTGTVNKIYDEVVAQGLPTLSTSDQKAEAAKQGNAF
                     QRAIRTFGDVFVPLLPAIVATGLFMGIRGAINNDTVLALFGTTSKAFSSSNFYTYTVV
                     LTDTAFAFFPALISWSAFRVFGGNPVIGLVLGLMMVNSALPNAWAVASGDAHPIKFFG
                     FIPVVGYQNSVLPAFFVGLLGAKLEKWLHKKIPDVLDLLLVPFLTFTVMSILALFVIG
                     PIFHSVENYVLAGTKFVLNLPLGLSGLILGGVHQIIVVTGVHHIFNLLEAQLIAADGK
                     DPFNAIITAAMTAQAGATLAVGVKTKNKKLKALAFPAALSAGLGITEPAIFGVNLRFG
                     KPFIMGLIAGAAGGWLASILKLAGTGFGITIIPGTLLYLNGQIVKYLIMVIGTTSLAF
                     VLTYMFGYEDKDEKAVAEVSPLVEETDDDPTITQTSQLRAETIVSPLDGQVIALDTVS
                     DPVFSSGIMGDGLAIKPRGNTIYSPVDGFVQIAFETGHAYGIKSDKGAEILIHIGIDT
                     VTMNGTGFTSKVKADQKVKKGDILGTFDSAKIAEAGLDNTAMIIVTNTADFADVIPLV
                     KEGVVTTGDMIIDVK"
     gene            42392..43831
                     /locus_tag="VBC94_000208"
     CDS             42392..43831
                     /locus_tag="VBC94_000208"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEO6978513.1"
                     /translation="MNLPTEIRYRPYDEWTEEDKENILKNVSKSPWRATYHLEAKTGL
                     LNDPNGFSYFNGKFHLFYQNWPFGAAHGLKQWVHTESDDLVHFKETGIKLKPDHVNDS
                     HGAYSGSALAIDDKLFLFYTGNVRDMKWNRDPRQIGAWMTSDGKITKFDKVLISQPND
                     VTEHFRDPQIFNYDNQFYAVIGAQNSKKCGFIKLYKALNNDIHHWEFVGDLDFGGTGS
                     EYMIECPNIIFVKGKPVLLYSPQGLDKNELDYQNIYPNTYKIGQYFDANSSKIVEPSP
                     IYNLDYGFEAYATQGFNTSDGRAFIVSWIGLPDIDYPSDQFDYQGAMSLVKELSIKNG
                     NLYQYPVPAMKNLRQHQAEFKTQLQTNNTYELELLVPRNDLSSFVLFANPKGQGLSIT
                     IDTAKGKVIVDRSQAGQQYATEFGTSRQCDIPKDATSINIFIDKSIFEIFINKGEKVF
                     TGRVFPDAEQSGIQLKEGHVHGKYFELKY"
     gene            43833..44795
                     /locus_tag="VBC94_000209"
     CDS             43833..44795
                     /locus_tag="VBC94_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6978514.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVTKVNEAMRTL
                     GYKPNNLARSLQGKSAKLIGLIFPNIRNIFYAELIEHLEIELFKHGYKTILCNSEKDP
                     IKEKEYLEMLGANQVDGIISSSHNLGIDDYEKVEAPIVAFDRNLAPHIPIISSDNFFG
                     GKMAAQTLKKHGCQKMIMITGNDNSDSPTGLRRLGFSYESKESKVITVTNGLSNMRRE
                     MELKSIISTHKPDGIFTSDDLTALLVIKLISQLGLSIPEDIKVIGYDGTSFIQDYVPH
                     LTTIKQPIREIAQLMVEILLAKIEGQKTNKDYILPVSLIPGSSV"
     gene            complement(44879..45313)
                     /gene="nusB"
                     /locus_tag="VBC94_000210"
     CDS             complement(44879..45313)
                     /gene="nusB"
                     /locus_tag="VBC94_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEO6978515.1"
                     /translation="MTSVFKDSRRDLRERAFQTLFSLETGGEFIDAAHFAYGYDKTVS
                     EDKVLEVPIFLLNLVNGVVDHKDELDTLISSHLKSGWSLERLTLVDKSLLRLGLYEIK
                     YFDETPDRVALNEIIEIAKKYSDETSAKFVNGLLSQFITNEN"
     gene            complement(45306..45695)
                     /locus_tag="VBC94_000211"
     CDS             complement(45306..45695)
                     /locus_tag="VBC94_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6978516.1"
                     /translation="MTTENLGEIVISPRVLEVITGIAATKVDGVHSLRNKAVTDSLSK
                     KSLGRGVYLKNEEDDTVAADIYVYLQYGVNVPAVSIAIQQAVKTAVYDMAEVKISSVN
                     IHVEGIVPEKTPKPDLKSLFDEDFLDD"
     gene            complement(45784..46344)
                     /gene="efp"
                     /locus_tag="VBC94_000212"
     CDS             complement(45784..46344)
                     /gene="efp"
                     /locus_tag="VBC94_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEO6978517.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNNETYDQYEIPTVNIENE
                     LLYILENSEVKIQFYGTEVIGVQIPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
BASE COUNT        14690 a         9281 c         7430 g        15134 t
ORIGIN      
        1 tgatcttcaa tttgaaataa tttaatatag aattcttgat tcattttaaa aaaagtcaac
       61 aactctgata acaactgaaa acctgagata tagtctaaat tatccgtaac ttgttcgctt
      121 aattctgttt ggaaaatcca ttctaataaa gcatatttat caacaaagtg attataaaat
      181 gtttgacgtc taatacctgc atcttccata atatcactca ccgatatttt gtcaaaagct
      241 tgcgaaataa ataaacgttt aaatgacttg gctatttttt tcttagtaat aagcgaagaa
      301 gtcataattt ctcctttatt ttatattgaa ttgctgatga tttattatgg tttagtgtaa
      361 catgatttat gattattgaa aaccctaaca aagcggacaa acaagaaaat ctgtccattc
      421 cagaaaaggc ttacaaattt tataataaaa gagaattaaa cataaaagga gtttccaaat
      481 gaaaaaaatt ctcaatcaac caactgatgt tgttactgaa atgctagatg gattagcata
      541 tgttcataat gatttagtac atcgcataga aggttttgat atcattgctc gcaatgaaga
      601 gaagtcagga aaagtggctc tcatttctgg cggaggaagt ggacatgagc cttcacatgc
      661 tggtttcgta ggcgaaggaa tgttatctgc agctgtgtgt ggtgcagtat ttacttctcc
      721 aactcctgac caagttcttg aagcaattaa agaagctgat gagggggctg gagttttcat
      781 ggttatcaaa aattattcag gggatatcat gaactttgaa atggcacaag atatggctga
      841 aatggaaggc atagaagttg caagtgtggt cgttgatgat gatattgcag tcgaagacag
      901 tctttacaca caaggaaaac gtggtgtagc tggtacgata ctcgtccata aaattctagg
      961 acatgctgct cgtcatggca aatcacttca ggaaatcaaa gctattgctg atgaattagt
     1021 accaaacatt cacactgttg gacttgcact aagcggggct actgttcctg aagtaggcaa
     1081 gccaggattt gttcttgcag aagatgaaat tgaatttggt atcggtattc atggtgaacc
     1141 aggttatcgt aaagaaaaaa tgcaaccttc aaaggcttta gcgacagaat tagttgataa
     1201 gttaattgaa tcgttcgatg ctaaatctgg cgaaaaatat ggtgtcctta ttaacggtat
     1261 gggtgcaacc ccacttatgg aacaatatgt ctttgctaac gatgttgcca aactccttga
     1321 ggataaaggc attgaagtga attacaaaaa acttggtaac tatatgactt caattgatat
     1381 ggctggttta tcactaacct tgattaaact ggaaaatcaa gagtggttag aagcacttaa
     1441 tagtgacgtt acaaccattg cttggtaatc attcctgtta attagaacaa aaggaaggaa
     1501 ctcttatgga agtaaaaact gcaatagaat ggatgcacac ttttaaccaa aaaattcaat
     1561 ccaacaagga ctatttatcc gaactagata ctccaatagg tgatggcgac catggtggca
     1621 atatggcgcg tggtatgact gctgttattg aaaaccttga taacaatgaa tttagtagtg
     1681 ctgccgatgt tttcaaaacc gtgtccatgc aacttttaag caaagtaggc ggagcatctg
     1741 gaccactcta tggttctgca tttatgggaa ttacaaaagc tgaacagagt gaaagtacca
     1801 tttcagaggc acttggcgca ggacttgaaa tgattcaaaa acgtggtaaa gctgaactta
     1861 atgaaaaaac aatggttgat gtttggcacg gtgttattga agcgattgaa aaaaatgaat
     1921 tgactgaaga ccgtatcgat tccttagtag atgctacaaa agggatgaaa gcaacaaaag
     1981 gacgcgcttc ttatgttgga gaacgctcag taggacacat tgatccagga tcattttctt
     2041 caggattatt atttaaagca ttactagaag ttggaggtgt ctaatgtctg atattggtat
     2101 tattgttgtt tctcactcta aaaatattgc tcaaggagtt gttgatctta tttcagaggt
     2161 agcaaaggat gtctcaataa cttacgtggg tggaacagaa gatggtgaga ttggtacttc
     2221 ttttgatcaa gttcaacaga ttgtagagca aaatgataaa aagacgttat tagctttctt
     2281 tgatttaggt tcagcaaaaa tgaatttaga gttagtagct gatttttcag aaaaaaatat
     2341 tatcattaac tctgtcccag tagtggaggg agcttacacc gctgctgctc ttctacaggc
     2401 aggtgctgac ctggatagta ttcaatcaca attagctgaa ttaactatca ataaataagg
     2461 agatattatg actcaatttc ttggggaatt tttaggaaca tttattttgg ttcttcttgg
     2521 ggatggcgta gttgcaggaa atgtgctcag taaaaccaaa gaagaaggaa ctggatggac
     2581 tgctattgtt tttggctggg gaattgcttg taccgttgct gtttatgttt ctggattatt
     2641 cagtcccgct catctaaatc cagcagtcac attagcaatg gcttctattg gagtaatttc
     2701 ttggggacaa gttatcccct ttatcattgc acagatgtta ggagcaatgg ttgcggcaac
     2761 tattttatgg ttacactatt atcctcattg gaaagaaaca aaagatagtg gacttatcct
     2821 tgccagcttc tcaaccggac ctgctatccg ccatacacca tcgaatctcc ttggtgaaat
     2881 tatcggtact gctattcttg tgattacaat tatggcaatt ggtcctagta aagttgcagc
     2941 aggtcttgat ccaattatcg ttggtatagt tattttcgca gtaggattct ctcttggtcc
     3001 aacaactggt tatgctatta accctgcacg tgacctaggg cctcgtctta tgcatgctat
     3061 cttacctatt gaaaataagg gtaactcaga ttggtcatac gcttggattc cagttgtagg
     3121 tccaattatc ggcggtgtcc ttggggctat cttatacaat attgctttga ctttagttta
     3181 actaaaaaac cagaaagtta atactttctg gtttttcata gttgcagtat gctttcaaaa
     3241 tagaaaaaac accaaaatac ttagtgttct ttctggagat tttatgaaaa aagtttagga
     3301 tgtttatagt ataaccgccc ttacttaacg tttccttaaa agctcggtta ttaacattac
     3361 ttcctaaatt actatttata ttctccaaga aatgactact attaagctaa aaagcgctat
     3421 tgacatagcg ctttttagtc tagtattatt tagtttcagt agtaactcca tcaaaatctt
     3481 cttcattttc ttcagcttct tctcttgtgg cacggataat accatctttg tgatgagctg
     3541 gactgtaaat tgtatagagt tttaacatct cagtttcaga agtgttaatt acgttatgtt
     3601 cagcacctgc cggaactatt atagcgtctc catctgcaac ttcatattca ttaccatcaa
     3661 tgacaacctt accatgacca gcttcaaaac ggaaaaactg atcattttct gcatgaatct
     3721 cgctaccaat ttctccacca acaggcagtg tcattaaaac gagttgacaa tgttctgcgg
     3781 tatagaggac ttggcggaaa tttgtatttc ctagtgtcaa tttttcgata ttcccatgaa
     3841 atcctttcat aacgatacct acctttctat gcctacagtc tactcttttt gctccttact
     3901 gtcaaaaatt tagataagag tctgacattt ttaataatat ctactgttcc ttgattccta
     3961 tcatttcatt gcaaacctaa aatatagcct aatgacaata gctatctata gctttagaaa
     4021 caaataatgc tgttccctgg cctccccaat tatcaataga cttttggaaa cgctcatctt
     4081 agatgtacat tatttcaaga cttgctaata aatcgttagt gcagttgtaa aactgagctg
     4141 tgatataatc ttgtaaaatc tgaacttgtt tttgaacttt ctcatctgtc ggagaaagtt
     4201 ctttcaactg tccaaagtct ttaaaaatct gactcatagc ttgtgaaatc attgaaaaat
     4261 cattcttact atggctattt tcaaattctt tgtaactatc tgtatccccc ccagcgtgtt
     4321 ctagcttcct cttgaaatac ttctaattct tcttgattat aagctgtaaa atctgacata
     4381 tttttacccc tctggataga ctttgcatgt tcaattactt cttctagtcg ctgtttcttt
     4441 agttccagcc acctaatttg atcaagaagt gcttgattac gatcataatt tggactttcc
     4501 attatctctt ttattttttt aagaggaaat tccagttcct taaataacaa aatttcttgt
     4561 aacttaatca gtgattcatc atcatagtaa cgataaccat tctctcctac aaatgaagga
     4621 gataacaaat ctatttcatc ataataatga agtgtgcgca cactaatacc agaaatgtga
     4681 cttatttctt taactgtttt cataataacc tctctgtaaa gagtataatg tataacgtta
     4741 agtaagggtc aagggaaaaa tattattttg tgatgattaa tttataaagc ttaaatcaaa
     4801 ggtcactttg actaacaggt tgataaaaag ggatacgtcc ccatgtttgc tctgctatat
     4861 tagcatttcc catctgataa acagaatctg ctcgttgcgt caaactatcc cacgtttgtg
     4921 aaccaatcct cgtcacgata tccacagact tcttcacaga ctttaaatgc tgttgacctt
     4981 tttgtgtaaa acctaagaca tgaatagcat ttggtatagg actttccaca gcatttatca
     5041 agatataggt taataaacgt cgaattcttg ctttggtata ccgtttagtt gcgactttat
     5101 caactaactc atccacagtc tccacataac gaatttgact ttttatacga ttggctatct
     5161 cttcattcac ttgataaatt tgtgtcaaat cagaatgcgt cattatctga tatttgagta
     5221 gactaaaata atcttgccaa actacttgtg gactattcaa aaacaagtca ctgttaggca
     5281 tacctttttc tacaaagaaa cgatcagatt gatgtttacg aagacttgtt gcagaagcaa
     5341 atatctctgt tttttctgat gaatgataac cagctccttg acgttgtatc ggatttaatc
     5401 gaataccatt ttggctagct gctttggtgt aggctaagcc taaaatgtga tttggggttt
     5461 gtcccgaaaa tgtaatccct gcaaatcctt gccacatagc ttgcattttt tgtggatagg
     5521 aaagagaatc cgattgtttt ttcaaaaaag cttccatctc ctctttttta ttcacataaa
     5581 tatccccaat acgggcataa tcaagcattt cttcggtacc gaagcataag ttatccacac
     5641 ctaatcgtgc caggatagac acagctccac tagcgaaata atccgctgac tgtaccgaaa
     5701 ccagaaaagg gagttcaatc actaaatcag caccattttc tagtgccatc tgggcacggg
     5761 tccatttatc tacaatcgcc ggctcaccac gttgcataaa attacccgac atggctatta
     5821 cttttatgcc ttgagcttgt tctaaaaggt acttatgccc attatgaaag ggattaaatt
     5881 cggcaacaat tcctgtaact gtcatacttc tttctctttc aattgattat ttaaagtaac
     5941 agcatcaata atatctaggt gataagtttt acgaaggtat atgagacgat ctgtttcttt
     6001 ttcaaatggt tttaatgccg ataataaatc caaatgaagt ttagggttca tcaataattc
     6061 caaaataact ttagactttt tctgacttac ccgacgttca ataatataaa gtatcccaaa
     6121 tatcatcaga caagcgtata agaatattat catcttttta cctccaaatt attacttatt
     6181 tatgagctac aaaaaaccag cgagcagaag ttgcagttgg ttttttatct tcaaaatctg
     6241 catacacctt aacatcttta aaacctgcct gttcaagtaa aatatcataa gtcaaaatat
     6301 cataagtacg ctcttcatga acttcatcat gacgtgtaaa tcgaccatct tcttcttgaa
     6361 caaaaaatgt taattcatgg acaatagaat gaggtgcatc atcttcataa gtatcccaca
     6421 ccatagcaaa gtcatctgca ttttcatgat aagaataacc aggaaatact ttatctgttt
     6481 gataagtcga atgaacatca aaaataaaga cgccattttc ttctaaggcc ttatagactt
     6541 cgataaaaac atcaccaacc tcgacttcat cttgcatata acaaatagag tctgaatagc
     6601 acgtgatgag atcatattta ccaacattag ataaatctaa catatttcct tcaataaact
     6661 ggatactttg gtgagcagaa gttgcacgtt ttttggcaag tttcagcata tcgccactaa
     6721 gatcaagtcc tgtaacagca taaccagctt gtgcaaatcg aacagactga attcccgtac
     6781 cacatgctaa ttccaaaagt tttttcttac cttttggaaa atgacgaagt gaaaaatcag
     6841 tccatttatc atataaagta tcatccatga cagcatcata aacagctgca aaagtttcat
     6901 acgttgccat ataatagttc tcaaatcttt cttgatatta catctctcaa aaagcagtcc
     6961 caagactgct tagctttatt ctgtcataaa gacttcagca tcaagcaatg gtgcttcatg
     7021 ccaaagtttt tcaaggttat agtgctgtct ctcatcttca gagaagatat gaaccacgac
     7081 gctattgagg tcaagaagta cccaaccagc tttactatcg ccttcaaggt ggctagcatc
     7141 cccaccattt ccttttactt gttcacggat attgtctgcg attgcttcta actgcctact
     7201 attacttgca ctcataatca caaagtaatc tgcaacactc gttacaggct gtaaatctaa
     7261 aattacaata tcttctgctc gtttttcatc cgcagcctta acgactagct gtagtaagtc
     7321 tttttctgtc attttattcc tcgtttatta atttcaataa gctatctttt gctaccattg
     7381 tcgcaaaacg atgatgccaa atattctcgt aatgtttttt aatagaatcc ccacttaaag
     7441 ttggtgtatc aaatgttagg tgtccacctt gtggaataaa gatatcataa ccatattcaa
     7501 aagcaacttt tactgatgta tcaaaacagt attctgtttg aagtcccatc atgattaatt
     7561 gactaatatg cttactttgc aagtaatcat gtaaatgagt atctttaaaa caactattgt
     7621 aagttttatc aattattttg tgatgcgtca caagatgtcg ctgttcccaa atttcccact
     7681 gtggagaacc ttgaggcaat tccttatcat gatgccttat ccatatcact tcacaattag
     7741 aacctttggc acttaaaagt aaatcatcta aaagctctgc aaaatttgtt aaatgctttg
     7801 gtttcttatc cataatacct tgttggatat caattagtaa aagtgcacaa gtcatacttt
     7861 cctccaaaat gaccactgca tcgccaaacc tatcatgacc attattcaat aaaagaatta
     7921 gtcgagataa ggtatataag cattataggt ttcaatcgtt ttaggataga taggctgagc
     7981 cttacttgct aaaaacgcaa ctgttctagc agtctcataa gctactgctt tattaaggtc
     8041 aactttcgct aactcacgag cttcttcaac tcctggaaaa tctcgattgt gttcaatgta
     8101 atctgctaca taaacgattt tatccaatgt ggacatttgg gctgaaccaa cagtatgttt
     8161 tgcaatagca gctaaaatat cttgatcttt gatagctaga tcctcttgta ttttataaat
     8221 gccaacaaga ccatgccaaa tattattacc ccattttttt aggtcgggat ctagttggta
     8281 tttatcaatt aatctaagaa attcatcatc agatagttcc ttagcataat catgtaataa
     8341 agcagctaga cccgcttttt ccttgtcata accataccgt tctgctaact ctattgcagc
     8401 acgctctact cctaacacat gattaaaacg tttgtcggac atcatatgac gcactttact
     8461 caaaagttca gtccgatcta aacctgtgta atctttgtag gtcatttgta aagaccttct
     8521 ttcctgatat aatctaatac ctctctaggc agtaaatagt tgggctgacg attactttta
     8581 ataaattgac gaatcatact tgatgagata tccatcaatg gcagatccac ccaaataacg
     8641 ggatatgatg taccagcttt atattttgga cgttgaacac caacaaactg caccatttta
     8701 acaagttcat caatacgatg ccacttaggt agatattcca ccatatctgc cccaataatg
     8761 aaataataat caacatctgg atttttttca attagaagct tcatcgtatc gtatgtatag
     8821 ctgatacatt tacgttcaat ttcaattggt tcaatagata aaccatcaat gccttctatt
     8881 gctaattcaa gcatcttgag acgatgctgt tcatcaattg tctctttttt atcaatatgt
     8941 ggtggctgaa attcaggcat caacagaact tggtctaaac acaattgctg acgtacttga
     9001 tctgcaacaa caaggtgggc gttatgaaca gggttaaaat ttcctcccat aattccaatt
     9061 tgttttcggt tagtatctcg ctttttctct tctaattcaa cttttgtaaa tggtgttagc
     9121 aattcgagtg ccataattcc tccatgaaca agtagtatct ctaaaaaaac aaacactgat
     9181 tatcatgatc agctatcata cggaagtgta acttaggtaa atcgtttttt agttctatac
     9241 tctatttctt aatctattgt agtttagttt gattttactg cttttacttt aactgaaatt
     9301 ttacgatttt ctttgcgcgc tgactcttta taaagaatca agatacgtcc tattttaagg
     9361 acagtatcgc agccaatttc atcttccaga acttcagcga catcgtggat atcttcatcg
     9421 gtattttgta aaagggttac tttgattagc tcacgtgcat cgagtgcatt acgtacgctt
     9481 gttttgattt gatcatttaa accatttttc ccaatttgaa taatgggttt catagaatgt
     9541 gcttctgatt ttaagaaagc acgttgttta gatgttaaca tattagtttc ctttttatct
     9601 attataggat tatgactgta tcatcagcta aattagatga aaaaccgtca tatgttgctg
     9661 gttaatttag ttattaaatt aaagctttgc gtaaaacaac tgccacgcct tctggagccc
     9721 aagcagctac tactactgga ttttctgctt ctgatttcac acgtatccaa ccaagacccg
     9781 aataaacaat atccatttta tctttgattg taaattcatg acgaactaat ttaggaaaat
     9841 ctgaaacttc cttacctgtt ggtggtgtca ataattttcc aacatgttta gtgtaaaatt
     9901 catctgctcc aactagttta gtgcggtgta aattcaggtt attatcaaaa tatgccgtaa
     9961 aaccttgttt ttgaccagaa ataaaatcaa aacgtgctag gccagccaag aataatgttt
    10021 gttctgaatt aagctgataa gttttgggtt ttatttcttt tttaggacta acatatttaa
    10081 gattttttgc tgtcaaataa tgagccattt gatgacgatg aataataccc ggggtatcaa
    10141 aaatatagct accatcatca agaggtattt ctattttatc taacgttgta cctgggaaac
    10201 gtgaggtcgt aataacatca cgactaccag taatttcacg aataatagcg ttaataagag
    10261 ttgactttcc aacattagta accccaacaa cataaacatc tcgaccatga cgatactttt
    10321 caatcgtatc aatcaaatcc ttaatagcat gatgattttg ggcacttgtc aagatgacat
    10381 caacaggacg aagcccctct tcatgagctc tctcagttaa ccattgtgtt acttttccat
    10441 ctttaaccga tttgggaaga atatcttttt tatttcctac taaaagaaca tcgtttcctg
    10501 caacgaaacg actaagacca ggaataattg aaccattgaa atcaaaaata tcaatgacat
    10561 tcaccacaag ggcatcacta tctccaacct catgtagtaa tttcaaaaat tcatcatcag
    10621 tgatatgaac gtcagtaatc tcattatagt ggcgcaatct gaaacaacgc tgacagtata
    10681 attctcctgt ctctaaacct ttttcaagtg ctgcacgagg tgtatagcca gcagcatctt
    10741 tattttcagt ttgaatcctg gctccgcagc caatacaaaa taactcttcc attaaattcc
    10801 tttttgatat acgattttac cgtaattctc ttctattttt ttccaaacgc gacgttcacg
    10861 taagcgattg aacttggtat tccaagcatc agatttaaca attggtttta ccaatacgga
    10921 ctttattcct gcgcgataac tagcacgaat atctgtcatc aattgatctc caaccataat
    10981 cacctcgtca cgatcaaagc cataacgctc aattgccatg tttataccac gagtaaatgg
    11041 tttcatcgca cgacttacaa aatccacacc aaaacgtgat actgcacgtt ctacgcgagc
    11101 atgattgtta ttagacacta ctacaactga aatatccgct gttgtcatct catctaacca
    11161 tgcacgcacc tcagcagtac catctggatt attccaagca atgagtgtat tatctaaatc
    11221 aactaaaacc gcacgaatgc catgacgtaa taaatcttct gctcttaaat cataaactgc
    11281 ctcaaccacg aaggtcggcc tataatcatc tatactcaat ttcatctcca aaaaatatat
    11341 tttactattc attctaccat aattcttaag agatagcgag taaagaaaaa aggtctttaa
    11401 agacctttaa attagggttt caaccccaaa agatactatc ttactcgttt cattttcttc
    11461 aatgcaatta aatctttgta agagattaga gttacagcaa gaaaaataaa cagcatccca
    11521 agtaaatcta ttggataaaa aatttctcct aaaacaagta ctgtcaaaaa taccgaagaa
    11581 actggctcta ccgatgctaa taagctaccc ttgacagcac ctacaatact aaccccttta
    11641 aggaaaaaag tgtaagcaaa aatagtccca ataccaataa tgccaatata agcaagtata
    11701 ctcatcacat taatttgtaa ggggtattgc cacgcttttg tcaccaaact aaataaaatc
    11761 ccacctataa acattcctga accaataaca atcgtacttc cccattcatg aataagtttc
    11821 acaggtaata aaatataaat agaataagta atggcggaac caagccccca cattaatcct
    11881 ttcggtgtga tagctaaact tcctaacttc ccatgcgttg caatcatata ggttcctaaa
    11941 attgccatag caattgctat tatttcaaaa gatgttggtc tttgtctgtt taggatacaa
    12001 acaatactca aaataagtat tggtgccata tattgcaaaa ccgttgcagt tccagcattt
    12061 gttaaatgaa tcgctctgag aaaagcatat tgatttaaaa ctaaaccaaa tatagaaaaa
    12121 agtaaaacct gctttaaaaa ttttggctgt ttccacgctg ctactaaatg ctccttttgt
    12181 tttgatctag ctaaagacaa gagaaagata cctgttatca acaatcgtaa agaagtcaaa
    12241 aggttaacgt gtactccgtg cgacatcaag tattgaccac taattccaga aatgccccat
    12301 gctagtccgg cagctaaggt catcattgtt cctttttctt ttttagtcat ttttcccctc
    12361 atttttattt cttaaacact gtagttaaag tcatctgata aatatcttag cttcttaatt
    12421 atttatccct aggtgttcgt gctaaattag cgaataaaaa ccagccaaaa ggctgatttc
    12481 tattcttctt ttaattttgc taattcttgc gctaacagct tcagagcaac ttgtggatta
    12541 gcttgtcctt ttgttgcttt cattagataa cctgtgaagg ccttatcagc attgcgtttt
    12601 cctgacttaa agtcaatcac agcagcttca ttatctgcaa agacttgatg aataattggg
    12661 attaatactt caggatctga aatttgaact aaaccagcct ttttaacgaa ttcttcagct
    12721 gaaccaccat ttttagctaa atgcacaaaa acttttttag caattttgga tgaaattgta
    12781 ccgtcagcaa tcaagccaat catctcaacc aaattctcag gagtcagacc aatttcttcg
    12841 attgattttg attcactgtt aagaaattgc gccacttctc cttgtaacca attggaaact
    12901 tgttttgcat cacccccaat agcaacagct ttttcaaaga aatcagaagt tgcttttgta
    12961 gctgttaatt ggctagcatc ataagatgat agaccaaaag acgatacata tttcgcacga
    13021 cgttcctgtg gaaattctgg taattctaaa cgaacttgat caatccattc atccgagata
    13081 tcaaatagtg gtagatctgg ttcagggaaa tagcgataat ctgatgagcc ttccttgaca
    13141 cgcatcaaaa tagtctcacc tgttgtttca tcaaaacgac gagtctcttg ctgaatttga
    13201 ccaccagatc gtaaaacttg tgcttgacgt ttttcttcat gtatcaaacc tttacgtaca
    13261 ttattgaatg agttaaggtt cttaagttct gctttagtac caaactcttc ttgaccataa
    13321 ggacgaagtg aaatattggc atctacacgc atagaacctt cttccatttt aacgtcagaa
    13381 attccagtat attggataat ctctttgaga gctgtcaaat aagcataagc ttcctcagga
    13441 gaacgcatat cagcttctga tacaatttca atcagtggaa caccttgtct attaaggtca
    13501 acataggaat aaccatctgt cccatgtgta ttttttcctg catcttcttc gaggtgggca
    13561 cgctcaatac gaattttttt tcgtgtacca tcttctaatt cgatttcaat ccaaccattg
    13621 taaccaatag gctcatcaaa ctgtgaaatt tgataagctt taggattatc tggatagaaa
    13681 taatttttac gatcaaaatg catgttttga tggatatcca tattcaaggc aagtgccgcc
    13741 ttgattcctg catcaataac tcctttattc atgactggaa ggacacctgg gaaagaccag
    13801 tcaattacat ttgtattagc attttgttcc tgaccaaaat gagcggaaga aggtgagaaa
    13861 attttagagt tcgtatttaa ttcaacatgg acttcaagtc caatgactgt ttcaaaattc
    13921 attagttctc acctccaaaa attttaggtt gttgtttatg ataatctgtc gttgcttcga
    13981 aggcagcagc aacctgatat attgtttctt cactgaattt cggaccaatc aactgcatac
    14041 caaccggtag accttgatca aaaccagcag ggattgaaat acctggtaaa ccagccaaat
    14101 taactggtat tgttagaata tcagctaggt acattgcaac tggatcgtga tttaaagaat
    14161 ctaagtcaaa ggctgttgtt ggagctgttg gacctaaaat caagtcataa tctgcgaaaa
    14221 ctttttcaaa atcttgaata atcaaactac gaacttgacc tgctttttta taataagcat
    14281 cgtaataccc agaagataaa ctgaacgttc ccagcataat gcgacgctta acttcgtctc
    14341 caaaaccttc agatcgtgta ttgacataga tatcatccaa gtttttataa ttttctgtac
    14401 ggtatccata acgaatacca tcaaaacgtt gtaagtttga agaagcttcc gatgaagcaa
    14461 caatataata aacagcaaca ccgtatttag aatgcggtaa actaacttcc tcaataactg
    14521 cacctagttt ttccagatgt tttgcggcct taataatcgt ttctttaact ccttgcgcta
    14581 ttccttctcc caaatattct tttggtaaag caattttcat tccttgaata tcttgaccaa
    14641 ttttagcagt aaaatcgcct acagtacgct cagaagaagt tgaatctctt acatcatgcc
    14701 cagaaataac tgtcaaaagc tgtgcattct cttttactgt ttgagacatc ggtccaatct
    14761 ggtcaagcga actaccgaaa gcaaacaaac caaagcgtga tacacgtccg taagttggct
    14821 tcataccaac gattccatta aatgaagccg gttggcgaat tgaaccacct gtatcagagc
    14881 ccaatgataa acgtacttgt cctgaagcta ctgctgctgc agaaccacct gatgatcctc
    14941 ccggaacttt actatggtcc caagcgttat ttgtcttttt aaaataagat gtttctgtag
    15001 agcctcccat ggcaaattca tccatgtttg ctttaccaat cacaatcata tctttagcat
    15061 ataacttttc aacagccgta gcatcaaaga ttggctcata gttatagagc attttagaag
    15121 cagctgttgt aagaatcccc tttgttgaaa tattatcttt aactgctaag ggaatacctg
    15181 acataacatt atcagcatca attcctttat catcaatttc tttggcttga gctattgcca
    15241 tctcatcaga aatagtaata aaagatccaa ccgcttgttc acgcgcatga atatcttcca
    15301 atgtagcttt tgttaattca gttgctgaaa tttctttttt aacgagaaaa tcatgcaact
    15361 gatcaattga ttgattatta aacgacatta ggcatctcct ccatcctcaa gaattgcagg
    15421 tactttaata taataatctt gatgctgtgg tacattttta aataagtcat cacgattatg
    15481 gcctggttga gcaatatctt cacgcatcac agttttgcga tctgccatgg tcgttgttac
    15541 tggtacgcct tctgtgtcaa cttcatttaa gagttcaatc atatcaacaa tctttgacaa
    15601 acttgacgca aattctttag tctcttgatc agaaaagcgc aattttgaca gatgggcaac
    15661 atgacgcact tcttcttcag aaattttcat gagttctcct tatctctgta tcattttcgt
    15721 tttaagatag tctctactat tatagcatat taaactctca atgttatcac attatcccag
    15781 cttttcataa taaacgcttc ttacctatga taagaagcgt ttactgttta tttatctatt
    15841 gttatatcat ttctgctttg tttgatttta gcctgtgcag ccgctaattt agcaatagga
    15901 atcctaaacg gcgaacacga tacataatgt aacccctgtt ggtaacagaa ctcaatagat
    15961 gatggttctc caccatgctc tccacagata cctattttaa ggtttggctt aacttctttt
    16021 cctaacctca ctgcctgctc aataaacctt cctattcctt tttgatctaa aacttggaaa
    16081 gggtcttttt ttagaagtcc tttatcaacg tattctccta agaattttcc agcatcatct
    16141 cgtgaaaatc caaagctcat ttgtgttaaa tcattggtcc caaaactaaa gaaatccgca
    16201 aactttgcaa tatcatccgc tatcatacaa gctcttggtg tttcaatcat tgtcccaata
    16261 gggaatgata agttaatttt tttagattta ctctcctcaa caattgtctc ttcaatcaga
    16321 tcacgtatta tagagatttc ttcatgcgct gtcactaaag gaatcataat ttctggcttt
    16381 gcctcatatc cctctttcat tgcaagtata gcgccttgta caagagctct cacctgcatt
    16441 tggtaaattt ccggataggt aatcgcaagt ctacaaccac gatgacctaa catagggttg
    16501 aattcttcta atgtttgaat ccgtttttca atagctaaag tagatatccc catttgtctt
    16561 gccatagatt ctatagactc tttatcatgg ggtaagaatt catgtaaagg agggtcaagc
    16621 aaacgaatcg tacaagcttt acctttcaat actttaaaaa tttgataaaa atcgtctctt
    16681 tggaaagata aaagcctttc tagagccttc cttcggctca atatttcatc cgctaaaatc
    16741 atttctctaa cgactggaat acgttcatcg tcaaaaaaca tatgttccgt gcgacaaaga
    16801 cctattcctt ctgctccaaa gtccaaggcc ttttgagcat ctctcggatt atctgcattt
    16861 gaacacacca tcatatctcg attttcatct acccatttca taaatatctc aaaatcacga
    16921 tctacgctag tctctgccat cggaacttgt cctatataaa catttcctgt agcaccatcg
    16981 attgataaca tctcaccttc ttttatcgtt tgatgcccaa tagaaatttc tcgtcttact
    17041 tcatcaacca ataattgact acagcctgca acgcatggtt tccccattcc tctggctact
    17101 actgccgcat gggaagtcat tccgcctctt gctgttataa tcccgtttgc actaaccatc
    17161 ccctcgatat cttcaggtga tgtctcttgt ctgaccaata gcactggatt tccctttttc
    17221 atctctttaa caacatcttc tgcatggaaa taaactttcc cataagcagc tccaggagaa
    17281 gctggaagtc ctgtcgtcaa aataatagct ttctttaaac ttttcaagtc aaaagaaggg
    17341 tgtaataact gatctaattg tttgggctcg atacgtagta tagcttcttc tttactaatt
    17401 agaccttcat ttacttgatc tactgctatt ttgatagctg ccttcgctgt acgcttaccg
    17461 cttctcgtct gaagcatata aagattacca ttttcaattg taaactccat atcttgcata
    17521 tcgcgataat gagcttctaa cttttgggta acgcttacaa actcttggta aataataggc
    17581 atttgttctg ccaattttga tatggattgt ggtgttctga tacctgcaac aacatcttct
    17641 ccttgtgcat taatcaaata ctcaccaaat aaatttgctg ctccagttga tgggtttcgt
    17701 gtaaaagcaa ctcccgttcc gctgttattt cccatattcc caaaaaccat agcttggata
    17761 ttaacggctg tcccaagcgt ctccggaata tcatttaatt tacgataaat cttagctctt
    17821 ggattattcc atgatttaaa aactgcctca attgctaaca aaagctgcct tttaggatct
    17881 tgcggaaatt tttctcctgc ttctttttga tatagttcct tgtaaaattc aacaagtctc
    17941 tttaaatctg aaccagttag ttctgtgtca tcttgataac atttatctgt cttaagtcga
    18001 tctaaaatgg tatcaaattt atattttgga atgcctgtaa ccacatctgc aaacatttgg
    18061 ataaaacgac gataactgtc ataagcaaaa cgctcgtctt ctgtcttttt cactaaacct
    18121 tggacactac gatcatttaa ccctaaattt aaaactgtgt ccatcatacc aggcattgaa
    18181 aaaactgatc ctgaccgaac tgacaccaac aaaggatcat caacacttcc taattgcttg
    18241 ttctgttcaa cctccaattg tgccattgct tgatcaattt gactaagaat actctctcgg
    18301 atatggcaag cgttatcata ataatcatta caagcctgtg ttgtaatagt aaaaccttgt
    18361 ggtaccggta acccaatact cgtcatttca gctagattag cccccttacc acctagcagt
    18421 tccttcattt ccttacaacc ttcatcaaaa tgatacacaa acttagtttc catcagcgtc
    18481 tccttttaca gatttttagc tacttgtcca tttgttttta agatttctaa aataatcgtt
    18541 gctgtttctt cgattgcttt atcagataca ttaatgattg ggcaacctaa atttttcata
    18601 acttcttcag catacctcgt ctcctccaaa atccgttcta aactggcata atttgccgta
    18661 ctagaaagac ctaaggactt taatcgaact tttcggactt gattaagatg atcaaccgaa
    18721 ttagttagtc cgataatcct tctagaatca atcatacgta attctttagg aacaggaacc
    18781 tctggaacca aaggaatatt aataacttta atatttttat ctgctaaaaa catacttaat
    18841 ggtgttttag aagttcttga aattcctata ataactaaat ctgcttgtaa aatacctcgc
    18901 ggatctctcc catcatcata ttttactgca aattctattg attctactct tttgaaataa
    18961 tcattgtcta agcgtcgtaa aatccctggt tctcccaaag gatctatacc cgatatcctt
    19021 gatattcctt gaataacatt tgttaataag tcaacataag cataatgttc actttcacat
    19081 cgtttctgtg cataggaagc caaagcaaca tcaactaaac taaacatcat aaatactgtc
    19141 ttctggctag cctcttcaaa aacttgttct aagcgttcct gactgttaat ataggaaaaa
    19201 cgttgaaaat gccaatcatc atgtcctgga aattgtgaca aacatgcttt cgcaatagct
    19261 ttggctgttt cccctaaaga atctgaaata ataaatatag tgagttgatc ttccatcagt
    19321 ttcctcctaa taatctctta tttctaaaaa caaactagcg aggattgttt tagataattt
    19381 cccgatcact ttaaattttt cgggatattg cttatcatga caaacgacag ggagactatc
    19441 cacttttctg ctcactaatt tatcagctgc cgcaaaaaga ctttcatttt ctaaaacagt
    19501 tgtcacgtgt ggcatacgtg tcatcaccat tcctattggc attttagaaa gatctcctcc
    19561 gccaatactg gtttttagta aatctttacg tgacacgact ccacagagaa aatcatcatc
    19621 atccaagata aaagcacaac cagcatcttc cataaaaata tgtacaataa catcataaac
    19681 tgaatctttt tgatgaactg tcagagggat ccccataatt tctgaaactg tcatcttttc
    19741 aaaatgactt gtccctattg aagcatgata ctgtcctaaa taaaaataac caaccttagg
    19801 ttttgcatct atgataccta gcatacttaa aaccgccaaa tcagacctta gtgctgctct
    19861 tgttactcct aatttctcag ctattagctc accagtaatg ggctgaaact gtttgataat
    19921 atcaataatt ctttcttgcc tttgatttaa ttgaataaga tcaccttctt tatatattgt
    19981 atactagtta ttttatatgt tatattatat atcttttttt actgaaatca agtaaaaaaa
    20041 gatactattt taaaaataat catactatat atttgctttt agacatatct tttgttaaac
    20101 tttcttcgaa aacgttttcg aatataagta tcggaggttg taatatgaca attaaaaatc
    20161 tcactgttgc aggaagtggc gttctaggta gtcaaatcgc ctttcaagca gcttacaaag
    20221 gaatgtctgt caccatatat gacattaacg atgaagcttt aaataaggga aaagaacgca
    20281 tcaaaaaatt agctaaagtc tatcaatcag aaattgaaac agcaaaagaa gcatatagtg
    20341 ataaagctaa aagtatcaag tataataaaa acttattgcc tagcttagac catatttttc
    20401 tttcaaaagt tgccgattca ttagacttga ttgctgattt accaaaccaa attacttttt
    20461 caaaaaactt agaccaagct gtaagtgacg ccgatttagt aatcgaagct gtcccagaaa
    20521 cagttagtat aaaagaagat ttttacaaac aattagctaa agtggcacct tcaaagacaa
    20581 tctttgcaac aaattcttca acacttgttc caagtcagtt tgctgatata acaggacggc
    20641 ctgacaaatt tttagcaatg catttcgcta acaatatttg gcaaaataat attgttgaaa
    20701 ttatgggaca taagggaact gacgacgagg ttgtcaaaga agctttggca ttctcaaaag
    20761 atattggtat ggtaccttta tatattcata aagaacaacc tggctatatt cttaattcaa
    20821 tactagttcc attcttagag agtgcattag cattgtacta taataaggtt tctgattctg
    20881 aaactattga taaaacctgg aagttaggaa caggagcacc gatgggacct ttagaaattt
    20941 tagatattat tggcatcgat accgcttata atatcatgaa gaattattca gataccacca
    21001 gcgatcctaa tagtttacat gctcaccttg caaaaatgct taaagaagaa ttcattgata
    21061 aaggacgtac cggtaaagct gctggacacg gcttttatga ttacgactaa actataaaag
    21121 aggttagata aaaatctaac ctcttttata attcaactaa agaatagttg caccaagaac
    21181 agttttaaaa tgatttaaag cccattgatg attactatca tttaagctgg ctacagcagc
    21241 tgctgggacc tcaattttat aacctaagtt ataagcatca attgctgtgt gtaatacaca
    21301 aatatctgtc agtactcctg tcaatatcaa agtatctact cgtctttcac gtaggcgaat
    21361 atctaaatca gtaccagaaa aggcagaata atggcgctta tctatccaaa aaacacgact
    21421 atcttcctta atagtttcat atagagttcc caaaggacca tataggtttc taccagaagt
    21481 ccctttaata ttatgctcag gaaaaagatt ggtctctgga tgaaaaacat ctccaatatc
    21541 atgattatca atcgcaaaaa aaatataatc ccctgaccgg tatgctttct ctgtaacgtc
    21601 agctatagct gaagcgatag attgcgctgg tttacctgcc gttaatttcc catcatctgc
    21661 cacaaaatca tatgtgtagt ctattgagat gagtgctttt gtcatttttc ttcttatctc
    21721 ctcttgattt caaaaaagga actgaaagct actcccagtc ccatgctatg attaattata
    21781 ttcttttaat ttgtcaaaaa taccttcgtt aataactttg aggtatgttc ccttcatacc
    21841 gagtgagcgg ctctcaatga ttcccgcaga ctccaattta cgaagagcat ttacaataac
    21901 ggaacgtgta attccaatac ggtctgcaat aacagatgcc gttaggcgtc cttcaagtcc
    21961 atctaattct cctaagatag cagctacagc tttcatttct gaataagata gagtgttgat
    22021 agccattgtc accgctgttt gtttacgaat attttcttct agattttctg tttgaaggta
    22081 aagtaattga ataccaacaa cggtgcttgc aatttcaact aaaatcaaat catcgtcgct
    22141 aaattcttta tcattacgcc agataataaa tgttcccaag cgcattccac caccataaat
    22201 tggagcgatt gttgtaatac catcttggaa gttttccttt gtctcaactg ggaaaattga
    22261 caaatcatta tctactgata agttagcttc tgtatcataa acacgactag cagatttaac
    22321 atagtaatct gggaattgtt ttgtttcaaa aaattcttcc acacggtcag tgtttgtttt
    22381 atatttcatg gcatagccaa gtaagttacc accaccatta ataatacatg cgttacagtc
    22441 aataatatca gctaattgag ccgccattgt attatatggt aattcggcat ctaagctatc
    22501 tacggaacgc tgtaagatgg atgtaatttt tctagttttt tctaataaat tcggcatttc
    22561 cttcatcctt tgtatgtttt gcgttacaat tatatcaaag cattttagct tttgcaaatc
    22621 gatatcagta cattttaaga acttctattg ttaatttaat gtttatctaa taaaaattca
    22681 ttctagcgct tgtattggct taaaaagcga gccatttttt cttgtagttc agtcaattca
    22741 tcaacacgtg gtaagtaaac aattctaaaa tggtcagcgg tattcatatt aaatcctcta
    22801 ccgtgtgtta gtaatacttt ttcttgtttt aagaaattaa gcacaaactc ttcatcatta
    22861 tcaatacggt acatatctgt atcaatttta gggaaaagat acagtccagc atttggtttt
    22921 acagcagata aaccaggtat ttcattaatt gctttatgga tataattacg ttgctcgtag
    22981 atacgacctc ctggcaacaa catactatca atagattgct gtccgcctaa cgatgtttga
    23041 ataacctgtt gagcaagaac atttgaacac agcctcatgt tagctaacat attcaaacct
    23101 tcaatataac ctttaacatg ttgtcgcgga ccagacaata ccatccaacc aacgcggaaa
    23161 ccacagatgc gatgtgattt agataagcca gataaagtaa ctgtaaaaat atcctctgca
    23221 atagaagcaa ttgggatatg ctccattcca tccataacta aacggtcata aacctcgtct
    23281 gaaaaaataa tcaagtcatt ttgtctagcg atatccacaa tctcttgcag aatttcacgc
    23341 ggatagactg ctccagttgg attatttgga ttaatgagga cgattgcttt tgttttggat
    23401 gtgatttttg atttgatatc atcaatatca ggataccaat tagcttcctc atcacaaata
    23461 taatgcacag catttcctcc agcaaggcta acacaagctg tccataacgg gtaatctggc
    23521 attggaacta gaacctcgtc atcattatca agcaaagctt gcattgacat agagattccc
    23581 tcactaacac cattaacaat gtatatatca tccatgtcaa catggatgtt ttgtaactgg
    23641 taatactgca taacagcctt acgagctgaa aaaatacctt tcgaatcaga gtaaccttct
    23701 gattcacgcg cattcgtaat taaatcacga atgacttcat ctggcgcttc gaagccaaag
    23761 gcagctggat ttccagtatt taaccgcaat atcttttcac catttgccct catgcggtcc
    23821 gcctcttcta aaacagggcc tctaatatca taagccacat gttccagctt cattgattta
    23881 tcaaatatct tcataaaaat acctcgtatc ttccattata aaacaaatat tctcaaaatt
    23941 gaaacattta ctgcggttta tcaagcgatt aagttaatat taagctattt cagaaaacta
    24001 acataaaacg ctttcatttt tctagaaaaa acgttataat atagctataa aatgaataac
    24061 caacatatct aacttactca tcactcactc cttctgataa gatggctagt tgagtgcaag
    24121 aggttatctc ttttggttat atttagagga ggacaggctt atgtcacaaa aatatgaacg
    24181 catccttgtc gctattgatg gatcaacaga atctgaactt gcttttgaaa aggcagttaa
    24241 cgttgctctt cgcaatgatt ctgaattaat cttgacacat gttatagata cacgtgctct
    24301 acaaagcttt gcgacatttg atacttatat ctatgaaaaa ttagaaaaag aagcaaaaga
    24361 tgtccttgag gaatatgaaa aacaagctcg tgaaaaagga gcagataaag ttagacaagt
    24421 cattgaattt ggtaatccca aaacattact tgcacatgat attccagaaa aagaaaaagt
    24481 agacttgatt atggttggtg caacaggtct taataccttt gaacgtttcc ttatcggatc
    24541 ttcctcagaa tacatccttc gtcacgctaa agtagattta ctcattgtac gtgaccctaa
    24601 taaaacaatg taaatttaat acgctcactt tctaaaatat cctgagttat attaactcag
    24661 gatattttta ttttcgattt aaggatttct gaatttggct atctaattca cgtcgaattg
    24721 atggctcagg agcgaaatgt tcttcccagt cgtctggttt taaaatttta tgagtaacag
    24781 gatcaaaatg agctttgcca tctggaaaga ttttacccat attggcctca tgtactgtat
    24841 caaaaagagg ttttgggtcg actcccataa gaacaaagga gccataagtc agataaagta
    24901 aatctgttaa ggcatccact tctcccacta atggtgtttc tggatggtct ttagaaataa
    24961 ccttatctct tgctttatca atagctaagt gtaaattacg gatagattgg tcaaatactt
    25021 cttggttacc ttgactagca gcatacaaga actcaacgat ttcttctact ttaaagtccg
    25081 acctaaaacc agcttcctta ctgtcatata agcgtggcgt tttaatagtt tcaccatcca
    25141 ttaatagatg gaaagatttt actttattga aattctcgtc acgactactg aatgtttttt
    25201 caatctcaaa ttggataagt ccataatggg ctaatgcctt agagatacca tcatcattat
    25261 tgtcagcagt tgtataacgt gcattttctt tcacttgctg actagcgttc cccatggcaa
    25321 caccaatacc aacttgagat aacatctcaa tatcattatc agaatctcca aatgctatca
    25381 cttctgataa atcaaaacca tagcattgac caacacgttc aattccctta acttttgact
    25441 gcccttcaga aatgacatcc attgagtaag gactgcttct tgttaattta acacgaggaa
    25501 attgtttttg aattcgctct gattcgccct ctgttgcaac taaaacgacc tgataaatag
    25561 gttctcgtaa gataaccatg aggctatcaa tattttgagg tttaatccga cgaataaaat
    25621 gcttaaagct tcgttcaatc gcttttgccc attttctcgg aacaaggcta gatacaatct
    25681 gccccaaacg gctagttcct agaccaataa tacctgaacc gagtaatcct gaggctgttc
    25741 ctaatgaaat ttctcttctt cttgctccag cataacgaat cagacgatag acagttgtct
    25801 tagataattg attggtataa ataatttcac ttctactata gatatattga ccattatagg
    25861 ttacagcaaa atctaaacca aggttttcta aaaaaggttg tacaaaacta ggacctcgcc
    25921 ccgttgctaa accgactagt attccttggt ctttcaaatt tcgaattgct ttaattgttg
    25981 acttttgtac attcttacga tcattaagaa gtgtaccatc aatatcaaaa aatactgcct
    26041 taattgccac tgggtcaaca tcccctcttt tttatgataa aataaattat atcacaactt
    26101 gaaagaagta taaaatcaat gaaaaaaatc cttgttctac acacaggtgg aacgatttcc
    26161 atgaatgcta acgagaaggg acaggtcatg tccagcgccg ataacccaat gaaatacgtt
    26221 gatttatcat tagatgacct tgatttgact gttgtagatt ttctaaatct tcccagtcct
    26281 caaatcaccc ctcatcatat gcttgatatc tatcactatc tgaagcaaca tgccagtaat
    26341 tttgatggag ttgtcattac gcatgggact gacactttag aagaaacagc atatttttta
    26401 gacacaatga tactccctaa aatcccaatc atcattaccg gtgctatgag aagcacaaat
    26461 gaacttggta gcgatggcgt ttataattac ctatcagccc tccgtgtcgc taatagtact
    26521 aaagctgccg acaagggggt gctagttgtc atgaatgatg agatacatgc tgctaaatat
    26581 gtcacaaaaa cacatacaac aaacgtttcg acattccaaa cccctacaca tggccccctt
    26641 gggattatta tgaaacaaga cctgcttttc tttaaagcaa ctgaagagag agttcgtttt
    26701 gatttagata aaattacagg aactgttcct atcgtgaaag catatgctgg catgggtgat
    26761 agtggtatca ttagcttttt aaattctcaa aatattagcg gattagttat tgaagcactc
    26821 ggtgcaggga atatgccccc aaaagctgca caagaaatcg aagaattaat tgagcaaggt
    26881 gtccctgttg ttctagtgtc tcgctgtttt aatggtattg ctgagccagt ttacggatat
    26941 gaaggtggcg gagcaaaatt acaagaatct ggtgtcatgt ttgttaaaga actaaacgct
    27001 cccaaagcca gactcaaatt actcattgca ctcaatgcag gtctcacagg gcaaaatcta
    27061 aaagactata ttgaaggttg ataaaaaact ctagaaatat ttctagagtt ttttattaat
    27121 tttcagtttg taatactctc tcaacactgc taataggcat ttcttgattc gttattaatt
    27181 gaaatgcttc tgctccttga taaatcagca tccctagacc attataagca tgtttcactc
    27241 cattttgtct aacaaatcgt aacaatgctg tttctttagg cttgtaaacc aagtcgacaa
    27301 ctactaaatt cggtgttaaa atttctggat cgttaattaa actataatta tctaatggcc
    27361 tcattcctaa actagtagca tcaatataaa aatgactagt tctaatagcg tctttaaatg
    27421 ctgtcttatt ttctagataa tcaacgacta cctttatttg aaactgtttt ttaattttat
    27481 ctgataagtc aatgacctta tcgtaatttg agctgttacg attaaataat ctaatttccg
    27541 caactccctc catagctgct tgaactgcaa ctgctttacc tgaaccacca ataccagcta
    27601 ttgtaattat tttattttta gcactgaaac cttgagctgc taaagcttta aaacaaccaa
    27661 tgccatctgt catatggcct actaaacgtc cggttccacc ttgattaacg atagtattta
    27721 cagcacccac taatttagct tgaggagata aatcatctag caaagggata acactctgtt
    27781 taaatggcat tgaaacatta acaccacgaa tactcaatgc cctgacacct cgaacagctt
    27841 ctgttaattt accctcttct acttcaaatg tcagataggc ataattcatg tttttttctt
    27901 gaaaagaggt attccacatt aacggggata gagagtggcg tgcaggattg gcaattagac
    27961 cagttagtaa agtttcccca tttaatgaat tcattgtata accttctttc ttaacaattt
    28021 atttcagttc attttaccaa aaactgactc aattaaaaag agagtatgta atttatactc
    28081 tctcccttag tttaaggcaa tatattacct gcactacggt aaatatcata ctattcttta
    28141 cgggttaaag cgatatcatt agccttatca atcgccttta aacgagatgg attcattgaa
    28201 ccaacaatcg cctgcatttt ggcaggatga cgtaaaatcc atgcaatcac aatagcttcg
    28261 ctgctaacat tgtagttttt tgccagttta gctatggttt catttaattc cttataatcc
    28321 ggatgattaa caaataaacc acgagataaa tcaatttgaa atggagacca agcttgaatg
    28381 gtaactcttt taagacgaca ataatcaaca atgccatcat catgttcaat tgaagcttta
    28441 tttaacatgt taacatttaa ccctgcatca aacattggtg tatgtgctgg cgataactga
    28501 agttgattca ctgctaaggg ctcgtcaagg taggattgta acaactccat ttgaaaccgg
    28561 ttttgattgc taacaccaaa gtgttttact ttaccttctg ccctcagttt ggtaaatgct
    28621 tctgctactt cttcaggctc aactaataca tctggacgat gaagaatgag aaaatctaaa
    28681 tattctgtcc ctaatctttc taaaatacca tctactgatg ataaaatata ctctttagaa
    28741 aaatcaaagt aaccttctcg tatcccacat ttagattgaa taatcatttt gtcacgattg
    28801 acatttaaat gtttgattgc atctcgaaag cgtaattcag atagccctcc cccataaata
    28861 tcagcatgat caaaaaaatt aatacccaaa tctaatgctg ttccaactac ttcttcagct
    28921 tgttttcctt tcaagtcact cattctcata caacctaaag caattcgagt tgcttgaatg
    28981 cctgtttgtc caatgttttg taacatatgc accccattct aatctgtttg tattaacaga
    29041 aagattaatc aattcattat aacaaaattt ctatgaaaag gcttactatt acaaatgcaa
    29101 aaagctgagt aaatttaact caactttttt tctgatactc attattgact aaataagtcg
    29161 gtaagaaaaa tctattctga aaagtactat tataaattac agtaatcatt attgtcataa
    29221 catacctcca catctgtgat aggaatatca tatgacagca agcttaaact gagcttagca
    29281 cagccttaat agaaacttaa tcaaaatctg aatgttggcg taaattatct agaataagtg
    29341 cccattctgt attttctttc caattattat ctttgacaat atcactagca acacgtctgg
    29401 cttcttctaa aataggataa tcctcaacga tatcagctac ttgaaattct ggaattccag
    29461 actgacgagt accaaagatt tcaccagaac cacgcatttt taaatccgac tcagctaaaa
    29521 cgaaaccatc tgtcgtttct gtcatgattg tcattcgttt tttcccagaa tcagttttgg
    29581 gattagccac taaaacagcg tatgattgtt tatatccacg accaacacgc ccacgaagtt
    29641 gatgtagctg gcttaatcca aaacgatcgg catccataat aatcatgatc gttgcatttg
    29701 ggacatttac ccctacttca ataactgttg tagatactaa aatatgactt tttttatcct
    29761 tgaaatcttg cattatagca tctttttcat catttttcat acgtccatgt acaagagcaa
    29821 ccttagcaat tccttcaaaa taagtagata attcagcatg caatgctact gcattcttta
    29881 aatctaaagc ttctgactct tcaatcaacg gtgaaatgac atacacttga gcatcttttt
    29941 gcaattcacc ttttacccat tccaacacag tacctagctg ctcgtgtttc acccagcgtg
    30001 taataatagg tttacgaccg gcaggtaatt catcaataat agaaacatcc atttctccaa
    30061 aagctgtgat tgctagagtt cggggaattg gggtggctgt catcattaaa acatcaggat
    30121 tttctccctt ttcacggaaa attctacgct gtttaacacc aaaacgatgt tgctcgtctg
    30181 taattacaag ccccagttta tggtactgta ccgagtcctg gataagagca tgagttccta
    30241 caatcatatc aaccgagcca tttgcaatag ctgctaaaac cgtacgctta acagctgctt
    30301 tcatacccga agttaatata gcgattgata aatctggaaa taactcttgc agactaatgt
    30361 agtgttgttc agctaggatt tccgttggaa ccatcaaagc cgattgaaaa cctgctgtat
    30421 aagctgcata cattgataga ccagcaatga ctgtctttcc tgatcctaca tctccttgca
    30481 ataaacgatt catatgagct cccgatgaca tatcagataa tatttcatct aaagagcgct
    30541 tttgagcatt cgttagaata aaaggtaaag atgaaatctt tgtctccata gcatgtttac
    30601 tataaagaat agacaaacca tttgtttcag atttattttc ggctttcaaa acttgaaggt
    30661 tcatttgaaa gtaaaataat tcttcaaatt taatccgacg gagcgcttgc ttatactctg
    30721 tgatatcttt tgggaaatgc atagctaaac aagcctgact acgacccatc aatcggtatt
    30781 ttcccaataa agtagctggt aaattttcct tcaattccaa atgcgcattg atttcaaaag
    30841 ctgacttaat agctttaatc aaagtagact gtgaaattcc ctgagatacg cgataaacag
    30901 gttgcatgtc atcttcaact tgagctaaaa ccttcatccc agtaatagct gatttagttg
    30961 catcccattt accaaagaca gctacctctt gaccaagttc tattttatca gctaaatagg
    31021 gttgattaaa aaaactaaca tttaagacag cttccccttg acgcaatttg aaacttaaac
    31081 ggttacgttt aaaaccataa tattgtacat tagctggagt aacgactaag cctgtaatga
    31141 ctgctttttc accatccact aaatcaaaaa cagattttga tttaaaatct tcatagcgaa
    31201 acggataata aagaagtaaa tcttctactg tataaatatc taatttctga aatttttctg
    31261 ctgattttgg tccaaatccc ttaagattag aaattggtga ttgtaatagc ataagattct
    31321 ccttctaata tcttttatta tactaaaaga tcaccaaaaa ggcgcctcaa gagcgccttt
    31381 attatgtcta attatttatg gccaataaca taagttaatt gtgcagcttg tgctgaacta
    31441 aaagtacggt aagagacaat accacgtccc attgcattag attctgaaat gagaactgaa
    31501 ccatcttttt taacttcttc aacaatagct acgtgaccgt aagtgccatc agcaccagct
    31561 tgtcctggtg aaaatgatac agcatagcct acttttggtg aatgtgttgt ttcaaagcca
    31621 gccttatgtt gccaatctcc accattaccc ataaatggat caaatgtata acctaactct
    31681 ttagcgcggt taaagacata ccatgtacat tcaccccatg gataagttga tgcaatatag
    31741 ctagctgtgt taaccgctgt tgataagcta aaaccacttg gtaaatctgc taaacttgct
    31801 gtcgataaag ctttcaaggc agcaggaatt tctggtgtcc ctccatgttg atcaatccag
    31861 cggtctaaat cttccataat ctgggcacga cgtttaccag ttccagagtt atctgtataa
    31921 taaacaccac cctcagtaac tgtattaagt tgtccagaag ataatttctg tgctttcaaa
    31981 tcttggattt caaagctaga gttgttgttt ttaataattg tattttttgt caacaacata
    32041 attgcttctt catcattata agcactagca tggctagagt catgatcaaa ggcaccataa
    32101 ccaaacatat tagcacctgg catcttagcc actccttgag ttcccaatga actttcaagg
    32161 acagcaatag ctacaatagc acggacatct aaaccactat ctttttccca ttgtaataac
    32221 ttagcaccat tgatacgatt agaatcatat tgaatcccta aagattttaa gtacccatct
    32281 aattgttcag ctttgatacc gtaacgatgt gacaataaat agtgagagta ggcatcctga
    32341 ccagaccagt gttctacgta gaatggcgtt gctgtcactg gtgaaatcgg tgttgataat
    32401 aagttatcat caaaggataa agcaggaaca gaagcattct gatttgtctg aacactttct
    32461 atctgagacg gagcgggagt tattttaggc tctactaatg gtaaggtagg ctcaggtttt
    32521 aattcagtct ttgttgattg acctggctta ctgtctgctt gactactgtt tgtagtatta
    32581 gtgctaggag tagatgctgt tgaagcagaa tcagatgttt tagcatcttc gttggaagaa
    32641 gattgatgtg atgaacttga tgatgattca ttgcttgatg atgtctctgt cgtaccaatc
    32701 tctgtttttt ctgttgagat acttccttcg gctgaaggtg aagcagtttc tgaatctgta
    32761 ggttgtgttt tttcctgttt ctctgcttca gaagaacttt ctgttgatgc tgttgccatt
    32821 gtcaggcttg tttctggatt aatagtttct gctaaggtca ctaaaggtga tgatagcgat
    32881 aagactaaag tggataacaa caaagttgcc gttacttttt gttttgacat atactaatga
    32941 ttcccttcta taaaaattgt cactgattag tctaacatat ttttaaattt tttaaaacag
    33001 ttaactactg tgtcatttgt ttttaataat atctcggaat acgatcgctt aaaagacaaa
    33061 gtacttcata attaatcgtc tgtctttttt gagcgacgtc cgtagttgta atgttacaac
    33121 taccttgttg accaatcaag gttactttag ttccgatagt atatttttga ggtaaacgga
    33181 tagtcatttg atccatcgag acacgcccaa taatttcaca aagttctcca tttactataa
    33241 ctgaaaatcc ttgcatatct cgtgtccatc catcagcgta gccaatcggt actgttccaa
    33301 caaattcgtc tccagtaact tgataggttg cgccatagcc aacttgagaa ccatcgtgta
    33361 gttgtttaac atgtactagc tcagactcta aacttaaagc agggtttata gggtaaggta
    33421 agtctaaatc agttccactt ggatttaagc cgtacataac aacacccaat ctaacggcat
    33481 taaaaattgt ctcactatgc caaaggcttg tagcagaatt actagcatga acaaggtccg
    33541 gacaattatc taatcctgaa ataaagtctt taaaaaaagt aagttgttga ttgaatttac
    33601 agttatcggc ttcatcagct gttgcaaaat gggtgaaaat ccccttgaca gaggcaccac
    33661 tttcccccaa ttcagaaatt agcttatttc cttcatctaa ctggcgaaca cctatacgtc
    33721 ccattccact atcaactttg atatgtactt ctagaccaga taaatctact gctgatgtct
    33781 gacataaacg taaccactcc aagctagcaa ctgttagagt aatattttca tttttggcta
    33841 gtataacctg ctcaggcata acaacaccta ataccaatat catttttaca attcctgctt
    33901 gacgtagctc aatagcctca tctaaattgg atacgcaaaa accatctaca atagactcaa
    33961 tgtattttga aacttcaaca gcaccatgcc cataagcatt agccttaact acagcaaatg
    34021 tttttttatc acttggaatg tgctcttgta cagatttaac gttatttgca atcgcctcta
    34081 aatctatcaa ggctctggtt ggtctatgat aacttgaaat catttttctt cctccaaaat
    34141 aacactagct tgtgcataat taccactatg agatattgaa acaaaagatt ttccattaaa
    34201 cggttctttt gtaatcaaag gcgctccttt atcatccgat aaaatttcga tatcatgaaa
    34261 attaactttc ccaattcctg ttccaagtgc tttgctataa gcctcttttg ctgcccatcg
    34321 ccctgttaaa aaagacatct gacgcttggg attggaaatt cctttaaaaa gaagcaattc
    34381 ttgttcggtc aaaacgcgtt ctgcaaaacg ttgattacgc tcatatgctt tagtaatcgc
    34441 ctctatctct tgtaaatcaa ttccgtgtcc aacaatcatg tgtaactcct ataagttcga
    34501 tacttacaaa ctattgtaaa tatccctaat tagcatttct gttttatccc aacctagaca
    34561 tggatctgtg atagacttac caaaaacatc tggtttatct tgtcttccat cttctaaata
    34621 agattctatc aaaaaaccac gaacatagtt tctaatttta gtatgccact gtcggtctgc
    34681 aagtactgat ttcacaatgc gaatttgttc taaaaattgc ttacctgaat tgtcatgatt
    34741 tgtatcaaca acaataaagg gattttgcaa atcagtctct tcataatgag taatagtttc
    34801 taaaaggttt tcataatgat aattagggat acttcgatag tcggcattat gatatcctct
    34861 caaaataaca tgtgacagca aattaccatc tgttctaact tgcttatttt gatagaataa
    34921 ttcctgttga ttttgtgctg catagaccgc attaaacatg actcttaaat taccagacgt
    34981 tggatttttc atacctactg gtgcatctat acctgaagat ataaaacggt gtccctgatc
    35041 ttccactgaa cgcgcaccaa tagcatagta agaaactaaa tcatccatca ctgataacat
    35101 ctctggataa agtaattcat cagctgtcag gagtccagtc tctcgaatga tgtcatagtg
    35161 catcttacgt gcttgaaaaa aaactcctag tttgtcagaa tggtgaacta agcctttata
    35221 accatcacca ttcgttcttg gcttagcggt ataaactctc ataaccaaaa acattttatc
    35281 tttaaaggca gcttccaatt tagctaaacg ctttgcataa gttagcaccg cctttggatt
    35341 atccgcagaa caaggaccta ctattactag aaaccgctga tcctcacctt ttatgatatt
    35401 ttttaactct tcttctttgg ctaatttttt ttggctaact tctagatcta agctagtttt
    35461 ctgcttcaat atttcaatat taattttatc acttaactga tgaaatccca tgttttttcc
    35521 taatacaacc taagtctatt aataagctct gctatacaat atagtcgtca tactattaga
    35581 caagtaatga taaaacagtc ttctcagact gttttatttt tattttaaaa tcattgttat
    35641 tgtaaacatc tcagaccatg acaattttta aacttcatgc cagagccaca aggacattta
    35701 tcattacgtt taatcccttg gtattctggt gattcattat tcataggtac atgttgtgcc
    35761 gagatatttt gctctgctgt tgtcgtagca tgttgagaag cacgttcacg ctcttgttca
    35821 tgaatttgag ctttcatcaa tgtacgtgtc acatcaaact caatcgaacc aatcatatct
    35881 tggaacatac ggaaaccttc tgattgatac tcaacgattg ggttattttg agcataacca
    35941 cgaagtccaa cggagttacg taattgatct aaggcatcaa tatgctctgt ccattgatta
    36001 tccactacca tcaagattaa gactttttgg aattcgataa tagcttctgg atcttttaat
    36061 ttagcaattt gttgctcata aacagctaag gcacgttcat aaagaagttc tttaatttca
    36121 gcctctttta gaccacgaag ttctgaaact gtgatagctt cttcatctaa taacgccgat
    36181 cttgcaaaat taacaatagc ttctgctgca gtatttttat cgcttctgct atgtgcatca
    36241 actgctcttt taatcgtacg tttaatcatc cctttgagct ctgatcctaa atcacgctca
    36301 gcagtaatca cttctctacg gttagcataa ataatctcac gttgttcacg cataacatca
    36361 tcgtattgca agacttgttt acgtgtgtca tagttattac cttcaacgcg gcgttgagct
    36421 gattcaactt gtcgtgtcaa catttttgat ttaataactg tatcatcttc agctaaattc
    36481 attcgctcaa gaaccacttt aatacgatct gtaccaaagc gacgcatcaa atcatcttct
    36541 aacgaaagat agaattgtga ttcacctgga tctccttgac gacccgaacg accacgaagc
    36601 tggttatcaa tacgacggct ttcatgacgc tctgtaccaa taacacataa accaccaagt
    36661 tcacgaaccc cttcaccaag cttgatatca gtaccacgac cagccatgtt ggttgcgatt
    36721 gtaacagcac cacgttgccc agcattcata atgatttgag cttctttaaa gtgattttta
    36781 gcattcaaaa cttcatgtgg tactccagca gcaactaatt ttctagaaat taagtctgac
    36841 gtttcaacag ctactgtacc aaccagtaca ggttgtcctt gttcataacg ttctttgaca
    36901 tctgctacca ccgctctaaa tttagagtca agagttggat aaagtagatc tgaatgatct
    36961 atacgttgaa caggacgatt tgttgggata ggaataacgc gcatattata aatttcacga
    37021 aactcttctt cctcagtctt accagtacca gtcattcctg ctaatttatg gtacatacgg
    37081 aacatatttt ggtaagtaat tgaagcgctc gtttttgatt cttcttggat tggcactgac
    37141 tcttttgctt caatagcttg gtgtaagcca tctgagaaac gacgtccttc catcgtacga
    37201 ccggtaaatt ggtccacaat taaaatctct tgttcttcac taaccacata atcaatattt
    37261 aacaacataa tatagttagc tcgaagagcg ttatcaatgt agtgagttaa ggcaacattt
    37321 tcgagatcat acaagttatt tagatggaaa tagttttcag ccttgtcaat acctgtatct
    37381 gataaaccaa ttgtttttgt tggaacatca atgatataat catcgctatt aagcgttttt
    37441 acaaacatgt ccgcacgtgt atacagttgg ttcatctcag agcttacagg acctgaaaca
    37501 atcaatggcg tcctagcttc atcgattaat acagagtcaa cctcatcaac caaagcatag
    37561 ttgagtgggc gttgcaccat atcttcttgg cgaacaacca tattgtctcg aaggtagtca
    37621 aatccgactt ctgcgttagt tgagtatgtg atatcacaat tgtaagcttc gcgtttttca
    37681 aatggtgact tagctgctaa gttaatccct acagataatc caagccaact ataaacctca
    37741 cccatttcgg tcgcatcacg tgttgataag tactcattaa cagtaataac atgaacacct
    37801 aatcctgaga tagcatttag ataaacaggc attgtcgctg ttaaagtttt tccctcacca
    37861 gtacgcattt ctggaatatc accatggtga agaacgatac cacccataat ttggacgtgg
    37921 tatgggtata aacctaatac acgtttagaa gcttcacgaa ctaccgcaaa cgcttccggt
    37981 aacaattgat ctaaagtttc accattttga taacgttctt taaattctgg agtttttgct
    38041 tgtaaagctt catctgataa cgtagccata tgatctgcgt aagagtcaac tttcttagct
    38101 attttatcta atttttttaa ttcacccttg tcattttcaa taacagtgcg tagaatattt
    38161 gccattctaa ttcctttcga tactcttatc accacatttt accataaata atgttgtctt
    38221 tcaataaatg gtcgttttaa tcgagattaa aacaagtggt atccccactc tcattatttt
    38281 ttaacatgac ttgctataat ttctaactgt ccatcaatat cccatttgac aacatcattt
    38341 ggcaagataa agtggtcacc ttttttaagt gtataaactt tgttatctac tgtaatgtga
    38401 cccgctccgt ctaagacact aaccaaaagg tagggtgcaa attgtttaaa gttggtaaca
    38461 ccagaaattt cccatttata aactgtaaag aaatcatttg aaactaataa agttgaagat
    38521 agatgtttta acttcatatt ggcaggaaca gtattggcag gtttaccaat cgtgagaaca
    38581 tctattgatt gttcaatgcg taaatcacgt aattttcctg aagcatctgg acgatcaaaa
    38641 tcataaacac gataagtagt atcactagat tgctgtgttt ctaaaattaa tatcccttta
    38701 cctatagcat gcattgtccc acttggaacg tagtaaaaat cccctgattt aacaggaatt
    38761 cgggttaaaa gatgttccca atctccagat tcaatcattt ggcgaagttc ttctttagtt
    38821 ttagcattat gaccatagat aatttcagat ccttcatcag cagaaataat ataccaacat
    38881 tctgttttac caagttcacc ttcatgttta agagcataag catcatccgg atgaacctgt
    38941 acactaagcc agtcattagc atctaagatt ttagttagta aaggaaagac agtatcatca
    39001 gggttgccaa ataagctttt ttcaccttga tacagcttat ctaaaaaaca acctttaaag
    39061 cgaccattct taactcttga gacaccgtta ggatgagctg aaatagccca atattctcct
    39121 gttgtttcac ttggaatatc atagccaaat tcatccctta actttgttcc accccagatt
    39181 ttatcgtgca tgcttgcttc taaaaataat ggttccgaca taagaatccc ctatcttttg
    39241 taaaattcaa gcctattata gcattttatc catcatatcg cttacttttt ttaatctttt
    39301 atttgctaac taaactaagt agtggcttaa gggttttatt aaccttttga tattttcttt
    39361 gctaaagcaa aatttcctaa cgttgcagaa ccattttctt ctatagctgg tgtcacaata
    39421 taatcagaca aatcgggaac tggtaaatac ccatttaata aagttgcaaa tgtctggcga
    39481 acacgtctta acatgtgctc ttgtgccatg acgcctccac caaatacaat gacttgtgga
    39541 cgatataata cagttgcttg tattgcagcc tgagcaatat aataggcttg aacatcccaa
    39601 accattgaat tttcttcaat caattcacca cgaataccag tcctagcttc tagagttgga
    39661 ccagatgcca aaccttctaa acagctatta tgaaacggac agataccttt aaaatcattt
    39721 gcttggtcct gcggatgcat cgccatataa gtatgacctg cttcagtatg accagttcct
    39781 cctataaatt cacccttttg tatagctcct gcaccaattc ccgtaccaat ggtgtaataa
    39841 acaagtgaat caatattgtt acgagctata acttcaccat aggcagagct attaacatct
    39901 gttgtgaaat aaaatggaac attgaaatct ttagaaatta agccgagtaa atcaacatta
    39961 gcccaatgta actttggggt cgttgtgata taaccgtatg tctttgattt tttatcaata
    40021 tcaatgggac caaaagaacc aatagcaact gcctctaact tcttttcaaa tcttttaaag
    40081 aaatcaacag tctttttaat cgtttcttga ggcgtcgttg ttggaaattg cattttttct
    40141 acaactttta attcctcatc accaactgca caaacaaact ttgtcccacc tgcttcaata
    40201 ctaccatata actttgtcat cttttttcct atctataatc tctatctcaa agacaaaggt
    40261 tgggataatt atcccaacct ttaatcctca cttaacatca ataatcatgt cacctgtagt
    40321 aacaacaccc tcttttacca atggtataac atcagcaaaa tcagctgtat tagtcacaat
    40381 aatcatagct gtattatcca gacctgcttc tgcaatcttg gctgaatcaa aggtgccaag
    40441 aatatcacct tttttcactt tttggtctgc cttgacttta gaagtaaacc ctgtgccatt
    40501 catggtgaca gtatcaatac ctatatgaat caaaatttct gcacctttat ctgatttgat
    40561 accgtaagca tgacctgttt caaaagcaat ttggacaaaa ccatcaacag gtgagtaaat
    40621 tgtattacct cttggtttaa tagcaaggcc atctcccata atacctgatg aaaaaactgg
    40681 gtcacttact gtatcaagag caataacctg tccatcaaga gggctaacaa tcgtttctgc
    40741 ccttaattgg ctagtttgag taattgttgg atcgtcatct gtttcttcaa ccaaaggact
    40801 aacttcagct actgcttttt catctttatc ttcatatcca aacatataag ttaatacaaa
    40861 agccaaagac gtagttccaa ttaccataat aaggtactta acaatttgac catttaaata
    40921 aagtaatgtt cctgggataa tggtaatacc aaaaccagtt cctgctaatt tcaatattga
    40981 agctaaccag ccacctgctg ctcctgcaat taatcccata ataaatggct taccaaaacg
    41041 taagtttact ccaaatatag ccggttctgt aataccaagt ccagctgaaa gcgctgccgg
    41101 aaaagcaagg gcttttaatt ttttattttt tgttttaaca ccaactgcca acgtggcacc
    41161 tgcttgtgct gtcatagcag ctgtaataat agcattaaat ggatctttcc catcagcagc
    41221 aattaactga gcttcaagta aattaaagat atgatgaaca cctgtaacaa cgattatctg
    41281 atgaacacca cctaaaataa gacctgatag gccaagaggt aaattaagga caaattttgt
    41341 ccctgctaaa acatagtttt caactgaatg gaaaatagga ccaatgacaa aaagtgctaa
    41401 aattgacata actgtaaaag ttaggaacgg cactaatagt aaatcaagaa catctgggat
    41461 tttcttatgc aaccattttt caagtttagc accaagtaaa cctacaaaga atgccggcaa
    41521 tactgaattt tgataaccaa ctactggaat aaagccgaaa aattttatgg gatgggcatc
    41581 acctgaggcc actgcccaag cgtttggaag ggcagagttg accatcatca atcctaatac
    41641 gagaccgata acaggatttc caccaaaaac tctaaaagct gaccaagaaa tcaaggcagg
    41701 aaaaaaagca aaggccgtgt cagtcaaaac gacagtatag gtataaaaat tagaacttga
    41761 aaatgctttt gaagtcgtcc caaataaagc taaaactgta tcattattaa tcgcaccgcg
    41821 aatccccata aataaaccag tagcaacaat ggctggcaac aatggaacaa aaacatcacc
    41881 gaaagtacga atagctcgtt ggaaagcatt cccttgttta gccgcttctg ccttttgatc
    41941 agaggttgac aaagttggca aaccttgagc cactacttca tcataaatct tattaacagt
    42001 accagttcca aaaataattt gatattgacc tgaattgaaa aatgctccct gaactttttc
    42061 gatattttca acagtatttt tatcaatgac tgtctcatct tttaccataa tgcgaagacg
    42121 agtggcacag tgtgctacgc tacgcacatt actagcacca ccaatcgcat taatgacttg
    42181 tttagcaatt tctgtatttg aagccatcta taaatctcct ttgacatttt gaaagcgttt
    42241 cattttattt actctaatat tctagcatgt ctcataaata tatgtcaagc gtttgacata
    42301 aaataatatt ttttgatgtt aagagttgat ttcaaaagag aaaacgttta ctatatagtt
    42361 attagatatt aagacagtca aggagtatca aatgaattta cccactgaaa tccgctatcg
    42421 tccttatgat gaatggactg aagaagataa ggaaaatatt ttaaagaatg tctcaaaatc
    42481 cccttggaga gcaacttatc acttagaagc taaaacaggg cttttaaatg acccaaatgg
    42541 cttctcttac tttaatggaa aattccatct tttttatcaa aattggccct ttggagctgc
    42601 acatggttta aaacaatggg tacatacaga atcagatgat ttagttcatt tcaaagaaac
    42661 aggaatcaag cttaaacctg accatgtcaa tgatagccat ggtgcatact ccggttctgc
    42721 attagccatt gacgataagc tttttctttt ttatactggt aatgtccgtg acatgaaatg
    42781 gaaccgtgac ccccgccaaa ttggagcatg gatgacaagt gatggcaaga ttacaaaatt
    42841 tgataaagtc cttatttcac aaccaaatga tgtcactgag cattttcgtg atcctcagat
    42901 tttcaattat gataatcaat tctatgctgt tatcggggcg caaaatagta aaaaatgcgg
    42961 attcattaaa ttatataaag cacttaataa tgacatccat cattgggagt ttgtgggaga
    43021 tttagatttt ggtggtactg gatctgagta tatgattgaa tgtccaaata ttatttttgt
    43081 caaaggaaaa cctgtattac tttatagtcc tcaaggacta gataaaaacg aattggacta
    43141 ccaaaacatt tatccaaata cctacaaaat cggacaatat ttcgatgcta actcatctaa
    43201 gatagttgaa ccatccccta tctataattt agattatggt tttgaagcct atgctactca
    43261 aggatttaat acttctgatg gtcgtgcctt tatcgtgagt tggataggct tgccagatat
    43321 tgattatcct agcgatcaat ttgattatca aggagctatg agtttagtta aagagttaag
    43381 cataaaaaat gggaaccttt atcaatatcc cgttcctgct atgaaaaatc ttcgtcaaca
    43441 tcaagcagaa tttaaaacac aacttcaaac taacaacacc tatgaattag aattactagt
    43501 tcctagaaac gatctttctt catttgttct ctttgctaat ccgaaaggac aaggactatc
    43561 cattactatt gatactgcaa aggggaaagt tatcgttgac cgctcacaag ccggacagca
    43621 gtacgccaca gaatttggta ctagtcgcca gtgtgacatt ccaaaagatg ctactagtat
    43681 caatattttt atcgataagt ccatctttga aatctttata aataaaggag aaaaagtatt
    43741 tacgggacga gtctttccag atgcagaaca atcaggaatt caactaaaag aaggacacgt
    43801 tcatggtaaa tattttgaat taaaatatta atatggttgc aaaattaaca gatgttgctg
    43861 cacttgccgg tgttagccca acgactgtat cacgcgttat taacaaaaaa ggctatttat
    43921 ctcaaaaaac agtcaccaaa gtcaatgaag ccatgagaac ccttggttat aaacctaata
    43981 atttagcacg tagcttacag ggaaagtctg ctaaattaat tggactcatt ttccccaaca
    44041 tccgtaacat tttttacgct gaattaattg aacaccttga aattgagttg tttaagcatg
    44101 gttataaaac tatcttatgt aatagtgaaa aggatcctat caaagaaaaa gaatacctcg
    44161 aaatgctagg agcaaatcaa gtagacggta tcatttcaag tagtcacaac ctaggaattg
    44221 atgattacga gaaagttgag gctcccatag tagcattcga tagaaattta gctccacaca
    44281 tcccgatcat ctcatctgat aatttctttg gaggaaaaat ggctgctcaa acccttaaaa
    44341 aacatggctg tcaaaagatg attatgatta cagggaatga taattcagat tctcctactg
    44401 gacttaggcg gctaggtttt tcctatgaat caaaggaatc caaagttatc acagtcacaa
    44461 atgggctatc taacatgaga cgtgagatgg aattaaaatc aattatctcg actcacaaac
    44521 cagatggtat ttttacatca gacgacttaa ctgctttatt agtaatcaaa cttatcagcc
    44581 aactagggtt gtccatccca gaagatatca aagtgattgg atatgatggt acaagtttca
    44641 ttcaagatta cgtcccacat ctaaccacta ttaaacaacc tattcgagaa attgcgcaat
    44701 tgatggttga aatactccta gctaagattg aggggcaaaa gacaaataag gattatattt
    44761 taccagtcag tctcatccct ggtagtagtg tttaggaaat catatttata aaataatttt
    44821 tagtaatagt tatttttcaa agcaaattaa aaatgtggaa gaatcttctt ccacattttt
    44881 aattttcatt tgttataaat tggcttaata aaccattgac aaattttgct gacgtctcat
    44941 ctgaatattt tttagcaatc tcgataatct catttagagc tacacgatca ggcgtttcat
    45001 caaaatattt aatttcatag aggcctaagc gtaagagaga tttatcaacc aatgttagac
    45061 gttctaagct ccatcctgat tttaagtgac tagaaatcaa agtatctaat tcatccttgt
    45121 gatctacaac accattaaca agattaagta aaaagattgg aacctctaaa actttgtctt
    45181 cagatactgt tttatcataa ccataagcaa agtgagccgc atcaataaac tctccaccag
    45241 tttctaagct aaatagtgtt tgaaaagcac gttcacgtaa atcacgtctt gaatctttaa
    45301 atacactagt catctaagaa atcctcatca aacaatgact tcaagtctgg ttttggtgtt
    45361 ttctctggaa caataccttc aacatggata ttgaccgaag aaatttttac ttctgccatg
    45421 tcatatacag ctgttttgac agcttgttga atagcaattg aaactgctgg tacatttaca
    45481 ccatactgca agtaaacata aatatctgca gctactgtat catcttcttc atttttaagg
    45541 tagacacctc tacctagaga tttcttacta aggctatccg ttaccgcttt gttacgtaac
    45601 gaatgaacac catcaacttt agttgcagca atacctgtaa ttacctcaag tacacgtggt
    45661 gaaatgacaa tttctccaag attttcagtt gtcatagctt atcctttctt ttagtgttta
    45721 ctaaggtttt tccttagtag agtaaattca tcatgaatct acttaacact tttttgagag
    45781 tcattaggcg cgtgaaacgt aagttccttc tgctgtattg attactaact tttgtccagc
    45841 ttcaataaag tctggaacgt taacaacaag tcctgtttcc atagttgcag gttttcctga
    45901 accagttaca gtagcacctt tgattgatgg ttgagtttca gcaactgtca attcaacagt
    45961 tgttggaatt tgaacaccaa taacttctgt tccatagaat tgaattttta cttctgagtt
    46021 ttcaagaatg tagagtaatt cattttcaat gttaacagtt ggaatttcat attgatcgta
    46081 agtttcatta ttcatgaagt aagctgtatc atccatctta taaaggtatt gagcaggtac
    46141 agtttcaata atagcttgct caaatttttc ttcaggacgg taacttgtat caaaagttga
    46201 accagtacga acatcacgca atttcatacg catgattgtg ttacctttac ctggtttgtg
    46261 gtggctagct tcaagaacac ggattaattt cccatcagcc gtttcaaaag tcatgcctgc
    46321 tttaagctta cttgcttcaa tcatttatta atacctcttt gtaaaaatta tttatcaact
    46381 cattttatca caaataacct gctttctcaa atagtttatt gatttggtat gtgtctgatt
    46441 tcttgataaa taaataaaat caccaagagg gcgtaaataa gactgaagca cttgacactt
    46501 tcctcttact tcagttttga taacagtttt aatat
//
LOCUS       DATROQ010000005        43856 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.005, whole genome shotgun sequence.
ACCESSION   DATROQ010000005 DATROQ010000000
VERSION     DATROQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43856
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.005"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            6..550
                     /locus_tag="VBC94_000213"
                     /pseudo
     CDS             6..550
                     /locus_tag="VBC94_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
     gene            770..1189
                     /locus_tag="VBC94_000214"
     CDS             770..1189
                     /locus_tag="VBC94_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HEO6978518.1"
                     /translation="MKMTVYFDGNFWLGLIEYDDDGDYKVFRYFFGKEPKDDDVFNFI
                     NHKLNDLIKKYEFVKTDISLKRTNEHKKSPKRMQREINREKRKPVVSTKAQLAMKTIH
                     MSIKNERQLSQKCKKNELRKHRYQLKQEKRYQKKKGH"
     gene            1389..1868
                     /locus_tag="VBC94_000215"
     CDS             1389..1868
                     /locus_tag="VBC94_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6978519.1"
                     /translation="MRDFEELFDKVKPIVMKLRRNYFVQLWEYDDWIQEGRIVLFRLL
                     EEHPYLLDNESKLFIYFKTKFSNYLNDVLRHQDCQKRQFNKMPYEEISEVSHYVKSKG
                     LVLDDYIAYRDTLTKVEETLSDIDKEKFEKLINGERFAGKKKFIRNIQPFFNAFKAD"
     gene            complement(2047..3144)
                     /locus_tag="VBC94_000216"
     CDS             complement(2047..3144)
                     /locus_tag="VBC94_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6978520.1"
                     /translation="MIEKYTKKDGTTAYRLRAYLGVDPMTGKQVRTTRQGFKTEREAK
                     RAEVKLIDDFQRQGAWKSNDKTTFDDVAKLWFEQYRNTVKPSTFLVNQNYYKTILKPH
                     LGQLQMTKITVMICQKFVNCLSRYSGYRLYLSLANRIFKFAVNLGIIDNNPMSKTLRS
                     KCTYKNMDTLTKKYYTKEELNAFLRIVEAEETLEMRLIYRLLSYGGFRIGELIALKDT
                     DFDFRNNTISITKTIAYTKEGWAVQSPKTKKSNRTISMDAETMTLAKLYIKQSIKPLH
                     GSFKLFNFASDTVRKRLDRFILKHGLKRITPHGFRHTHASLLFEAGIPAKIAQERLGH
                     AKIAITMDLYTHLSKKSKDNVADKLAELVAI"
     gene            complement(3317..3931)
                     /locus_tag="VBC94_000217"
     CDS             complement(3317..3931)
                     /locus_tag="VBC94_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978521.1"
                     /translation="MKKPFYKKWYFWTHAILLLVLGFSFFVIYRLAETNIVNEKKIAK
                     LEKTQENKTTSGIRKTISDFTSRFDEELSVRAIKFYLNKDQVVSSFGDEVKLGGGYLT
                     INKPNNDKTRMLATTTDFKNKIIVPIEFKNTTGETKGFDTRDIFAYNGDETISFDSVI
                     SENLDNDGYSVVVKDGETAAASIVFGTNSKIKDIKVRYNSGLWK"
     gene            complement(4063..4842)
                     /locus_tag="VBC94_000218"
     CDS             complement(4063..4842)
                     /locus_tag="VBC94_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6978522.1"
                     /translation="MSSKKLENKEIFSKNLAFYMERKRVDRNTLCADLGLKYTTVRDW
                     LKGITYPRIGKIELLAEYFSINKSDLIEEHSTNGATSKVNFDPRQAILLSNYSKLNNV
                     RKNKLLAISETLLSEEQGKVINLQEKMAEYGSRKRVSLSVPGKVSAGTGYWQEDDYDT
                     EVDFYADEIPDEKNYDTVAVVVGHSMEPKIKNGDFLFIKLADQVDINKIGIFQVNGEN
                     YVKKLKNDYLQSLNPKYADITPAEGDDFRTIGEVVDIYREG"
     gene            5203..5352
                     /locus_tag="VBC94_000219"
     CDS             5203..5352
                     /locus_tag="VBC94_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978523.1"
                     /translation="MSEQNEKFIGVLKDMHMQILRDFVNAEDPLTRCELAKGFLEIGD
                     YLSKQ"
     gene            complement(5341..5553)
                     /locus_tag="VBC94_000220"
     CDS             complement(5341..5553)
                     /locus_tag="VBC94_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978524.1"
                     /translation="MKISNSKDLALAIVASSSPTLSIEDKIKLYEDSLEAIKQHNLPF
                     IEAEKQEQINNGKVIAEALERGESLF"
     gene            5612..5770
                     /locus_tag="VBC94_000221"
     CDS             5612..5770
                     /locus_tag="VBC94_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978525.1"
                     /translation="MNWKKLMFGDLEHTFTSRNGKEKTSIEFEGGLLPALLVLGGITW
                     LIAWFITK"
     gene            complement(5868..6509)
                     /locus_tag="VBC94_000222"
     CDS             complement(5868..6509)
                     /locus_tag="VBC94_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978526.1"
                     /translation="MNFFNFLLCVFKFTSEYLIKNWIALIALFLSYSNYRRNNLQVEL
                     IAAPVSDWILSVILDNGESIYNPNGTLRANIKIINPSNVDVSYFDLIVFDKNRKYQHY
                     YQKQNNIINDLTGREAIAAVQPDGNTILIEVPEADCGVLKAHSMTRMDLIIQTSEITD
                     RLFVAFKVAKKKKLFKANKAGYVNSPYQSFSASFPVELSKKPHYEDILKDLHE"
     gene            6567..6770
                     /locus_tag="VBC94_000223"
     CDS             6567..6770
                     /locus_tag="VBC94_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978527.1"
                     /translation="MEEILSSIAKSLESIATEFKAQNSYREEMKQNMEQMEKIILDIQ
                     SDPFGLKHLKDKALSEKASKQKG"
     gene            complement(6754..7113)
                     /locus_tag="VBC94_000224"
     CDS             complement(6754..7113)
                     /locus_tag="VBC94_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978528.1"
                     /translation="MDKLTNDAKFLLSSMFVKYNERRKDKISKEKSRNFEDIQFIKEN
                     IMNEWSEEDVLDTCFELRKHGYISATAASDTLYLISLTTEAIAELEKQDQAKTLSGRI
                     EYWLEFAKKIKDAIPFA"
     gene            7186..7392
                     /locus_tag="VBC94_000225"
     CDS             7186..7392
                     /locus_tag="VBC94_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6978529.1"
                     /translation="MTKMTLKALRATKNWSQEEAARALKVSKDTWGNWERGNTEPSVT
                     KAYQIAYIFDISLDDIIFLPNIAV"
     gene            7446..8405
                     /locus_tag="VBC94_000226"
     CDS             7446..8405
                     /locus_tag="VBC94_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978530.1"
                     /translation="MQEITTQNDFDYSLVDFETQEFLQERSNIIYGIQSKSAYEIGKQ
                     LVKAQEVLAKNRYGCFEEWYSSLGFRKTKAYEYINHYNFIRSQSEQLNIETFEELPKK
                     LQSEMSKPSANPELNQKVFDGDITTHKQYKELERQLKLAEADKERLKQQNERLAEQAL
                     SAKVVEKEVVIEKVPDDYESTKQLNRTLLDKNKELSTTLEEAEWELDSKKLELSTIKL
                     ESQRAIEVTNQIRHLEGKKEKLENLVTSISELSSIISDVQNFFDTKMAPLRFKPIINN
                     VNAHYSVTEVTKMVNTVQTWCDEMYKIIPSGNRKTVEEVIINE"
     gene            8398..8919
                     /locus_tag="VBC94_000227"
     CDS             8398..8919
                     /locus_tag="VBC94_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978531.1"
                     /translation="MSKELSEMNEKDALEVVKGMTIEDMMIEVLSSQKQVKASQEAIK
                     KDVEEVRSLAIEIDKKVHIDDVEASEIKSIISRQSYEFAKEYFDNRKVVPSANLFASK
                     KGQFIRVQHSRLKHHFNVTKYTHIKHTEAEKAISYLKSLSYGLFTPFETRETPKQKEI
                     IELENKNNGDDVA"
     gene            8938..9129
                     /locus_tag="VBC94_000228"
     CDS             8938..9129
                     /locus_tag="VBC94_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978532.1"
                     /translation="MNEKITDEQLNKYLEIRTELGEENFKFINACYDEVLHLKKEEAT
                     KNIKFEITDFSKFRDFAKK"
     gene            complement(9157..9333)
                     /locus_tag="VBC94_000229"
     CDS             complement(9157..9333)
                     /locus_tag="VBC94_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978533.1"
                     /translation="MISSQRIKETLDRHDVSESDKLSVALAEILNKHLSGKNLSETVH
                     EHDKRMARMRGEIM"
     gene            9448..9624
                     /locus_tag="VBC94_000230"
     CDS             9448..9624
                     /locus_tag="VBC94_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6978534.1"
                     /translation="MKKKLNKILIDKGMSKKELSEKTGISYNTIMNIGKRDISFNKMK
                     KIADVLDISLNEFR"
     gene            9703..9960
                     /locus_tag="VBC94_000231"
     CDS             9703..9960
                     /locus_tag="VBC94_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978535.1"
                     /translation="MQSQLTYDLLKKQIAEELFDEFKELIQEKDLANRWVNQTALVNE
                     YGYSWQTIKRMESYGLKSFKNGKDKMYCLADVNEIKHLMKQ"
     gene            9990..10133
                     /locus_tag="VBC94_000232"
     CDS             9990..10133
                     /locus_tag="VBC94_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978536.1"
                     /translation="MKLFNFIFAKPKKQEEKPKWTIETHGWEANARRYDNMIEHNNRG
                     RTC"
     gene            10209..10409
                     /locus_tag="VBC94_000233"
     CDS             10209..10409
                     /locus_tag="VBC94_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978537.1"
                     /translation="MNNDWKKRYINNQIDFFNDIKTCVKERWPNMKGNDVDKISIAIY
                     RNWPVSDINSTLEVIETDLEKL"
     gene            10406..10696
                     /locus_tag="VBC94_000234"
     CDS             10406..10696
                     /locus_tag="VBC94_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978538.1"
                     /translation="MKCYVNKQKKLAIDMNYKDKFGKFSSDSIQILEGKLTDSIQIDV
                     ENAMKEIIDKYSQLFDTPIIDDLFTEKEKQLKQSYDVETTLTEMFEVEYEDN"
     gene            10683..11366
                     /locus_tag="VBC94_000235"
     CDS             10683..11366
                     /locus_tag="VBC94_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6978539.1"
                     /translation="MKITKATEITKTHNWRILIYGKPGNGKTYLINYLKGKTLILDMD
                     HSSKTIAGNENIDIIQFDRTHPSDFMTEFLTELPELIKEYDNLVIDNITSWQSDWFIE
                     QGRKSKNGITNELQQYNMWTNYYLRVLTTIYSQPINIFVTAWESTQDLTLESGQMITQ
                     YVPDIRKQVLSQTLGLTDVVGRIQVNPKTGGHGILLEGSDGLYAKNRLDNRTVCKAEE
                     LFNFEGSDA"
     gene            11402..12805
                     /locus_tag="VBC94_000236"
     CDS             11402..12805
                     /locus_tag="VBC94_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6978540.1"
                     /translation="MNEARRHILKHNVMIVSPPGSGKSVVISDIAKSATQKNGYVLFL
                     VHRKELIDQITNSFKFHGIDMNKVDLMTVGKAKNRLDKLTKPTLIITDEGHHGKASTY
                     QMIYEYFSDVPRIGFTATPWRLSGDGFTDTYDVMVLGKTVEWLINNNKLAPYDYYSVL
                     SIDTAKLKVQNGDYSNKSIDESFGKKIFGDVVQEYIKKANGQKAILYAHSVEASQAFA
                     KEFQSMGINAIHADAKTPKAKRDKSMKDFRDGKIQVICNVDLISEGFDVPDCTVTILC
                     RPTKSLVLFLQQSMRSMRYQPNKKAIILDHVGNWNIHGLPDTPHHWENYFRGGWKKKS
                     NKTNTVHAKECPVCSALWPLSQQLCELCNHDFGLKEKQEKERIEAELELIKRERFRIK
                     QLANKKFGKDLKTNWEIAQARVKDAGKGKPLYKLIYFYLKTDWVETNVNELAEVTGKS
                     EKEIYSAYNWLKKKLRG"
     gene            12810..13292
                     /locus_tag="VBC94_000237"
     CDS             12810..13292
                     /locus_tag="VBC94_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HEO6978541.1"
                     /translation="MAGFTTDFSEVKEHAEFKEQPYEMIVYDAYEAVNDRNGKKRVVI
                     DYVVRNDIKQEMQNYHLWDEQYPNSQTGKYHIGILMGKAKALGIKEGQHYDSFEAFLN
                     DFKGRTAKVTVKLDEYNGNKYPKVRYANQSDVPESHHIWKEKTTGFTQAEIEEDDLPF
                     "
     gene            13310..14863
                     /locus_tag="VBC94_000238"
     CDS             13310..14863
                     /locus_tag="VBC94_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HEO6978542.1"
                     /translation="MTSNEFIEALSKLTTETDWGDPIFGESVLKAELRKHLFKIVPID
                     HNGYIHKLFYSEMVKDEDVMYFVSDGRKTYRFLFGDTILKTDKQGNEYLTYSVENNFP
                     PFAKLVIDYILGTYTFFENKLYDIRYKQFKLIDDFTLQTKYGFKDSGHILEILQGIHK
                     TLNIQPINYIEPYQIACKDFIIDLENSEIINQPPLQNVSYFKYYEVDYKTAINSKSIA
                     EEYLEYVIADSNSLNNAILQSYFIAQVACGVRPKTNFFISKSGVRTGKGLRHIALSGL
                     FNKIDVELDTLKSNGFEALQAWAMFSGGEMALATEQGDIQGNAMERVLKIIATEKTHV
                     ARAIGQNQSIVNLTSVLCIDTNRTVALSDEMNGRKVLIQFKDRPKTETDYERESVFRK
                     YWLAFTDRDKNPKIDGCIGFLLNSLERFQKIGKWYQWKDVEVFNDIDLDEFQVALINA
                     LQEVDFVQRTDNKEVIDLSLQVYGKSNHAVSKAISEIGVRSRSKKVNGKTVRGYEIEN
                     KTRFDKYIL"
     gene            15132..16001
                     /locus_tag="VBC94_000239"
     CDS             15132..16001
                     /locus_tag="VBC94_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEO6978543.1"
                     /translation="MYHTTALSFLKKGYQVIPLRKDTGTPMIKFKDIQITEEVIKNTN
                     WFNCDYALLMRGIWCIDIDTHDMDEKLAKELYIMIKKMGIDLLSVLSTDKYDNGLDGY
                     SSIIRHEYKNELISNFKNTFAELTASGGMHILFKKRDDINYTQKIGVMPGVDIKANDN
                     NFVKIFPSDGREVLQAVKTLPYYDGKFEEEVFKPKQEGITTYFGCSVTYTSNGSHEGR
                     EAYERVATGTSYNRNDDLFKGACWAFENGIDIDDLTSIIGTVKGRDVFTREEFELTIE
                     SAKRKVSYVTIRT"
     gene            15955..16302
                     /locus_tag="VBC94_000240"
     CDS             15955..16302
                     /locus_tag="VBC94_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEO6978544.1"
                     /translation="MNQRNEKSATSLSEHDIQNLIRMELSQAGYMVFRANVGKVKTAD
                     GRFFDTGLPKGFCDLFGFKPNGQIFFIEVKNETGRIRPEQKNFMEVMASKGALAGVAR
                     SVEDALKIVNGYH"
     gene            16381..16677
                     /locus_tag="VBC94_000241"
     CDS             16381..16677
                     /locus_tag="VBC94_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HEO6978545.1"
                     /translation="MADKINAESMQAAYNENYQMFLAKNADYGNSFEKSLNDFGFIAG
                     IVRISDKYNRLYNLISSDKNVSESLSDTLNDMANYCMMLAVWLEKTENAHDTRS"
     gene            16661..16819
                     /locus_tag="VBC94_000242"
     CDS             16661..16819
                     /locus_tag="VBC94_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978546.1"
                     /translation="MTHVVRVYDHIGGRVLPTIYKDKEFKTKDEAIAYRDSLIAKSDA
                     EYFWRDEL"
     gene            16816..17232
                     /locus_tag="VBC94_000243"
     CDS             16816..17232
                     /locus_tag="VBC94_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978547.1"
                     /translation="MIPKFRAFNKETQKMYSIDGFKSSERKIYRCSLADDEFRSGRLE
                     TFHFVEDNLDDYILMQSTGMFDKNGVEIWEGDIVLTTRLIDYTYKNFKGVVKMLEGRW
                     LIDTGKDAVGLWTEVDENEAIGNIHENPELLERLDG"
     gene            17229..17393
                     /locus_tag="VBC94_000244"
     CDS             17229..17393
                     /locus_tag="VBC94_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978548.1"
                     /translation="MTKEEVIAFLTEQRDLRLIGYEWGKDNLSDFERWQLAQANMFLD
                     VIEWIEEVTK"
     gene            17390..17659
                     /locus_tag="VBC94_000245"
     CDS             17390..17659
                     /locus_tag="VBC94_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978549.1"
                     /translation="MTEQQMIDCLLYELAKKDKLNIRRNNIITFLSIVLMAISILNVA
                     LQDHYKSQITELRTQLSRTQKQLKRASDDRARQTKRIAELTGNGG"
     gene            17662..18147
                     /locus_tag="VBC94_000246"
     CDS             17662..18147
                     /locus_tag="VBC94_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6978550.1"
                     /translation="MSDIKILDACCGSRLFWFDKNEEHTTFMDIRQETFDIHGKHVNV
                     KPDVLGDFRNMPFENNIFNLVVFDPPHLKYVGQKSIMKAQYGQLDKDNWKEDISKGFE
                     ECMRVLKVGGTLVFKWSDCQINVKEVLSAIPFTPLFGQRRGTTHWMTFVKFAELTGNG
                     E"
     gene            18149..18298
                     /locus_tag="VBC94_000247"
     CDS             18149..18298
                     /locus_tag="VBC94_000247"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978551.1"
                     /translation="MIKVSKKEGKSVHIENNGDKAIVFVNNGNDVTIVSTEQSMTVNN
                     KRGIK"
     gene            18295..18828
                     /locus_tag="VBC94_000248"
     CDS             18295..18828
                     /locus_tag="VBC94_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEO6978552.1"
                     /translation="MNIEEAKKLIDKQSIGKGGVGDIPVVKTHIVKVLLDQLIQPKPE
                     VPQFVADWYEKHKDSLECDLYLYHMSIYDEEVEKDDFYYWMQTSKNPVYTLINMHQFG
                     YTVEKEKLYTVEIPNPNERQLSFVLMRQLSGNVSIKVMHRDNLDLLKTDNDLQLTESE
                     IRKDFDWAWQFREEVAE"
     gene            18825..19091
                     /locus_tag="VBC94_000249"
     CDS             18825..19091
                     /locus_tag="VBC94_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978553.1"
                     /translation="MKEKTIFISKEYANDFNNDKYNLSSGYYFRNGKKHDIAIVKYGE
                     KDYLKNTDLAYVVCDKIVDADSIGFVYHGEYETWHFKLLNTEAN"
     gene            19481..19915
                     /locus_tag="VBC94_000250"
     CDS             19481..19915
                     /locus_tag="VBC94_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEO6978554.1"
                     /translation="MTFFPEINIQKTKSNAKRKLREYPRWRRIANDVDTQKVTATYSF
                     EPRQSHGTPSKPVERLALNRVSAEQELEAIEQSVSMILEPEKRRILYDKYLSPYKNAD
                     KVIYTELCMSESFYYDTLDAALLAFAELYREGSLIVEQGVFD"
     gene            20081..20159
                     /locus_tag="VBC94_000251"
     tRNA            20081..20159
                     /locus_tag="VBC94_000251"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            20539..20877
                     /locus_tag="VBC94_000252"
     CDS             20539..20877
                     /locus_tag="VBC94_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO6978555.1"
                     /translation="MRPQKLTIAGGRRTTVDYDDRSAEYRDYNRNRWKYDKQVKQFYN
                     SKLWRETSKQVLLQNDYVCAMCGGEATMTDHIVSVKKDWNRRLDWNNLQASCKACNDS
                     KAIRERCKNN"
     gene            21048..21515
                     /locus_tag="VBC94_000253"
     CDS             21048..21515
                     /locus_tag="VBC94_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEO6978556.1"
                     /translation="MGRKLKVVETTKKHLTKEEKIARETAQNKASDGLDKLQITPPRH
                     LNEVARAEYRRIINDLQTLPVRNLDRGLLELYCSWYAIYKETTKKLDEIGYFTNDPDK
                     GLIPSPLILTLEKATANIRSSASQLGLTVDSRMKMFIPKEEEKQKSIFDKFGG"
     gene            21530..23284
                     /locus_tag="VBC94_000254"
     CDS             21530..23284
                     /locus_tag="VBC94_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEO6978557.1"
                     /translation="MTIEYDYSAISDIYKDDTFYYAKKIVDEEIKASKKVFKACLRHL
                     NDLKRIEDEDFKFIYLPQKASDPINFIEILPDVKTGKPYPLAMFQKFIIGNLYGWRKK
                     TDHSLRRFRKAMISVARKNGKTILIAGILLYEFLFGHNPSMSRQLFCTANDRTQAKIA
                     WDMAKKQLASLRAKDADVRKATKIVRDELKNLHDESYIRALSRDTGAVDGFEPYVGVL
                     DEFAASKTNEMLELLESGQGQLDNPFILIISTAGMDLNVPMHTIEYPYITKILDGEIT
                     DEGYFGYVAEQDNEEEIKDETNWIKSNPILEVDTLHDKLMDYLRTRRKVSLETGEVNK
                     VLIKNFNMWRQSSEESYIDKQSWELAKIDKPDTYKRRVWLGVDVGRVSDLFAISPVVM
                     MDDYWYVDSFSFVATKYGLTAKEKRDGVSYSNLERQGYCEITTLESGVIDDERVLEKI
                     EELIYINEWEVHGICFDPYQFGTLLTMIEKRHPEWPLIEVSQTTMVLNMPTKQFRDDL
                     KKGKIKHSGNPLLTMAANNAYIKTDNNGMRIDKNKNSNKIDPLDAVLDGYAVCYLEPF
                     DGSGYWTSEKILGGETLF"
     gene            23281..23448
                     /locus_tag="VBC94_000255"
     CDS             23281..23448
                     /locus_tag="VBC94_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978558.1"
                     /translation="MIGFILKNIHTLILLVGLGLLIYGLFLFGDKVGFIASGIILVIL
                     AIYVDSVGGRK"
     gene            23445..23675
                     /locus_tag="VBC94_000256"
     CDS             23445..23675
                     /locus_tag="VBC94_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978559.1"
                     /translation="MNKRIKKKRKLEHYIASLIAENVMFSKELIKQHERIEQLEKIVE
                     HNAQATNNELSRIKKHSKKKWKNNVTLPIERR"
     gene            23682..24902
                     /locus_tag="VBC94_000257"
     CDS             23682..24902
                     /locus_tag="VBC94_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO6978560.1"
                     /translation="MSFFQPLGTSKVSYDDYIASVLAGDVSQQYLGVSALKNSDVLTA
                     TSIIAGDIARFPLVKKDINGDIIHDEDINYLLNVKSTNNASARTWKFAMAVNAILTGN
                     SFSRILRDPKTGQALQFQFYRPSETTVEETENHEIIYTFTDTLTAKEIKCFAHDVIHW
                     KFFSHDTILGRSPLLSLGDEIDLQTGGINTLIKFFKDGFSGGILTMKGAQLSGEARQR
                     ARQDFEKMREGSVGGSPLVFDSTMEYTPLEIDTNVLQLITSNNFSTAQIAKALRVPSY
                     KLGVNSPNQSVAQLMEDYVTNDLPFYFDAITSEIGLKTLNDGDRRLYHVEFDTRSVTG
                     RNVDEIVKLVNNQILTPNQGLVELGKQKSKDPNMDRYQSSLNYVFLDKKEEYQDQVGV
                     KGKRGEVNAKENKP"
     gene            24880..25545
                     /locus_tag="VBC94_000258"
     CDS             24880..25545
                     /locus_tag="VBC94_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEO6978561.1"
                     /translation="MPKKINLKGPLIANNSQEVYDYYGMEAVSAKSIIDKLPEDNSDI
                     ILEVNSNGGLVTVGSEIYTALRNYKGKVTAEITGMAASAASVAVMGADKVVMSPTAQM
                     MVHKALFNWVSGNSDDLDKASSALKSSDKAIVNAYVAKTGKSEDEIMQLMRNETFMSA
                     QDAVESGFADEVMSFEAIASIDNQMLPQAVIDDYYASRSKRKQEINNMLLEIEKEEIL
                     QGL"
     gene            25569..26753
                     /locus_tag="VBC94_000259"
     CDS             25569..26753
                     /locus_tag="VBC94_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEO6978562.1"
                     /translation="MFDEKIEEIKADITELNKVIAAKTAEVKAALESDDLETARSVKA
                     EVEATKAKLVESENDLKLYESSVEVGGAENIGGKEVTQEEKTYRESVNDFIRSKGKIV
                     NDSLRFEGKDEVLMPINETTPVEPKKDGVKKTDVKPVSSEEILYTPAREVKTVVDLKQ
                     FTSIHQAKKASGKWPVLQRATEKMVSVEELEKNPKLGKPQFTNVAWEIKTYRGAIPLS
                     QESIDDADVDLVGIVAETIGQMKVNTTNDAIATVLKEFEAKTVKNLDEIKKLLNVELD
                     PAYNVSFIVSQSFYQTMDTLKDKNGRYLLQDSITSVSGKVFLGKPVFVLADEVLGKDT
                     AFVGDFKRGVLFADRKDLGLRWADNEIYGQYLQAVLRFGVSKVDDKAGYYVTFKPEQL
                     PS"
     gene            26767..26928
                     /locus_tag="VBC94_000260"
     CDS             26767..26928
                     /locus_tag="VBC94_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978563.1"
                     /translation="MAQPEVSSATVVGKPTKDSTIEDIKKYLTSQNIDFSGKTLKADL
                     LKLAGVEEV"
     gene            26931..27233
                     /locus_tag="VBC94_000261"
     CDS             26931..27233
                     /locus_tag="VBC94_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEO6978564.1"
                     /translation="MTVSKELLDSVKLYCKIDFDFEDDIIEEMIASAQEQICFAIEEG
                     STPEMFKNYAKFSLAVKKQVKEEYDHRGLSADTNRYPLANGVLNIIHQLRLRGDGA"
     gene            27230..27577
                     /locus_tag="VBC94_000262"
     CDS             27230..27577
                     /locus_tag="VBC94_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEO6978565.1"
                     /translation="MITRKMNVRIAIFSQTGGQNEDGEVVSAIRKDLYTCWAEVSKTQ
                     LRDFNYQSRYQNASNLPTNKDTKVFLIRYNPKLSIDNTMFIEHNKHIYKIDKIEFDES
                     GKEMIILSGVSIS"
     gene            27574..27951
                     /locus_tag="VBC94_000263"
     CDS             27574..27951
                     /locus_tag="VBC94_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO6978566.1"
                     /translation="MTKGLDEILANITKLEVKSPKIAKEAVTQVAEEFEKALTKNTPV
                     DYSVHDTKLKYDTAISGFKGANVGITSKKIGYGRKTGWRAHYPNNGTIYQKGQDFKEK
                     TINEMTPRAKEIYAKKIKEGLGL"
     gene            27948..28373
                     /locus_tag="VBC94_000264"
     CDS             27948..28373
                     /locus_tag="VBC94_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978567.1"
                     /translation="MIAETTAYKLLKNDRTLNELLDKLRGGPFKNGFDQGIFTYDIPD
                     NPVDLRKVELAPFMRINTTYDGPSLYADDDMISNEQRITINFWCKTASQSDQIASCID
                     RILKNGGFERYTANEKPRYKDSDIDLLMNVRKYRYFDFY"
     gene            28389..28985
                     /locus_tag="VBC94_000265"
     CDS             28389..28985
                     /locus_tag="VBC94_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6978568.1"
                     /translation="MGKVKFGLRDFQYAVLGDNDKVNEKDKGIKALPGMKSAKLDITN
                     ELVTVMADDGPYVVLSGGITETKLEIEVLDLTSEARQDFFGIKADKGIERYNKSLTPN
                     NVACMFRTSDENGKAIWVGLLKGKFNIPGMDTKTKEGAPNPEPDKVTGNFVARGTDGD
                     VVVIGREGVNGFELKKFKELVFDGVTGLEKSSEDNSLM"
     gene            29038..29358
                     /locus_tag="VBC94_000266"
     CDS             29038..29358
                     /locus_tag="VBC94_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978569.1"
                     /translation="MYEITLKKGGVDKTFSKDFVNVEDNMLAVEHQVRQSAIFSDDKR
                     RLDDKEHRKLNESYLQMFVEMYGNQFNVDDLKQSNVSVLDKLTELFVDALGGEKEEDE
                     KKEQ"
     gene            29388..29555
                     /locus_tag="VBC94_000267"
     CDS             29388..29555
                     /locus_tag="VBC94_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978570.1"
                     /translation="MWIQSLLKNGYTILDIKKMRLSDIELMVKALETDFAEKEEVVET
                     TLDKAFPFLFG"
     gene            29568..33509
                     /locus_tag="VBC94_000268"
     CDS             29568..33509
                     /locus_tag="VBC94_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEO6978571.1"
                     /translation="MGNIGDLVATATLDISPFMSNTRNLKTYMKTLDNSLKAVENSFN
                     GHGSRVKGLKAIYTETGGALKSYQELLKKQSQKYNELKENIGDVNKATASQKQALINA
                     KSAMMETAAKVSELQSRLKALGAETSVFTRFGNAAEQAGKKMKSFGDSVSGVGAAFTK
                     GVTAPIVAGAGYALKAAVDYEDAFAGVKKTVDEVKDSNGKVVYSYDMLSKGIRQMAKE
                     IPASTTEISHVAEAAGQLGIKTKDVLGFTRVMIDMGKSTNLSSEEAATALARFANITQ
                     LDPSKYSNLGSSIVELGNNFATTEKEIVEMGLRLAGTGKVVGLTDPQILGLATAMSSV
                     GIEAEAGGSAFSRVMQKINTQVLSGGEDLWKFAKIAGKSADEFAASWKKNPQEAIIDF
                     VKGLKRFKEEGKDVTAHLQDMDINSVREIDTLQRLAGAGDLLGDAFKSANKGFSENKA
                     LMNEANKRYETTASKLQMLKNQVNDVAIEFGGPLIDALRDGLEAGKPIIKGVADLAKA
                     FSSLDKEQQQQIIKWGLIAAATGPALSIFGKGVGVIGGTIQGLGKLSKTLGTLSGSLG
                     AAKTGAAALGVGAEGATTALTGMSGAAALLGNPVTWGVVLGGAAALAVGYFAQKAYEA
                     HQRTQEWGTKVSQVQANELQSFKDKVDQTNQSMEGFRGGAEQVNSVKTAFQGLVAEIE
                     KLENKDLSEKIKLAEQFGFSQGTMDQIKKSSRQTVENVKQMSDEVINIYQNASNEHRR
                     LTEEEKAVVLANQNELINVQLSKLNYSAKEKKAIVKAMNGDLNSLNSQQLTKALEVTE
                     KWIKAENKSYNKLKSGLKKVYDSIKGNDKEAVKTREEIHKKQQQLEADHYLKMEAYGK
                     RYAAIQKKLLKGTAKYLDPQLQQAMVNDVKKQMKELGLSYEELMTKTTKAASKAQEVN
                     TMWARTTKKSTEDQKLANSQWNGLVWNPKTGKLKTNAKEEVAKALEAEGGWDRLKFIA
                     KNANLETNARITMAEVLVETGKWDTLTPEEKELVVDGHQGIQAIVESKQHLEIWNSLP
                     EEVKRILGDNKDFLNKKGVATRTLENWNALKPDEKKLLAKNLTKKGKDEAQKTINSLV
                     GKEVKVTAANKTLSGVNSAQRTLNSVQDKHVTIWASIKKTASDLWSKLTGYAVGTDYH
                     PGGLAMVNDQKGSLYKELVTLPNGQSFIPDGRDVILPLPRGSKVMKASATRDYMYDLG
                     IPKYANGIGFDNTKIANITQRMSELPKNTVTSTTDDKLYWMIEEMIAVLKSTKDNSVI
                     EQALDIAEQAIERPVELYLQDGQWVAKVADRITNYQTQRNSRNNRMKGMLN"
     gene            33506..35029
                     /locus_tag="VBC94_000269"
     CDS             33506..35029
                     /locus_tag="VBC94_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEO6978572.1"
                     /translation="MKFIYDGVDMSKFFRISRVERSIGNERTLSLNETFQLGTAIRGI
                     KTGAKIIKVHIEPLEINGVLTEQLKHELAGVLNVDKPKKMTFGDEPDKYYLGLAQGEI
                     STEKVARWYQRAVITFYIPDGVAHSITYKKFSDYTQEGNKLIFNLQNDGNTNAYPIIK
                     IKHNSENGYIGIANETGAFALGSSEEEDGTIVHRNESLFDYSKAIAQALEGAPNVAKL
                     NYMPPTYDTELKRMRIDNILGSGKGGEYVVIGNRGTTPGYTEHIGTRTFIINPDSNGE
                     YTLNEHLWWQQIFIATAQDQKGFLKLCVTGIDDEGNDEFLYGIETYKRKNGFETEYNF
                     FALDDDGVGWRFYKQFKFQADRNYHNPFSMNRSRAVEIFREEDKFRIYFNGAHHHVTV
                     PSLKGKKSRKIHLAMGTCSDSSKYINYNLFEKVNFEKMGVSHYNNIVNKYQPGDEVVI
                     NFENDTVKTKDLDSIQDMVLGSQPISIPPGKTEFVMQLSKFSQYAPNVEILMEERWL"
     gene            35020..38841
                     /locus_tag="VBC94_000270"
     CDS             35020..38841
                     /locus_tag="VBC94_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6978573.1"
                     /translation="MVIITLVIHDSKLHPVLLLDNEKQGTLNYFDDTWTRQLTTGSSV
                     FEFSVYKKTLEGDNPLNHKYQVLNDQAFVSFVHKDKVQLFNIMQIEETETTVRCYCEN
                     LNLELLNEYCNAYKATKAMSFEEYLVQFDILNWGALTIGTNEVKDKKLTLEWTGQDTK
                     LARILSIANNFDAEIEFETQLHNNHTFKAFIVNVYKEYEEGVSYGVGRDRSDIVLRYQ
                     KNVTGITKKLDKRQIYNAIRPYGKKTVKGERVISNPVTRKVTKTVGSNRTYLGGDLKY
                     YGHTIKKANVQSIINYAVQYNILPSGIICQLYLESLWGDSAVGKRDNNWAGISGGAQT
                     RPSGVKVTTGMARPPSEGGTYMHYASVDDFLKDYTYLLAKQGLYNVVGKKNIADYTKG
                     LFRVGGAKDDYAAAGYQSYTNLMTNIRNGINKVTGNILNTIDKLWQTPVQPITAVNVA
                     RRATKTMQALNEATRLKGRRIGSGQCYALSGWYAKKLDGAWIDSSIGGIRGRIGGGMA
                     AALIGTDYNWGAYGWKLDRSPNAGNLQAGGIYNVKANFGAPFYTTQWGHTGIIKSVSK
                     TRVTVLEQNFVGRMYVVENSYDINSFASGLQTVCYPREIAQGMAVNGATTQQVSGGTQ
                     ISYEEVVQEAQTESYEEEQIIYIDNSIYKEWKDENGKVEYYLKNGFLYAPLSRDRYPS
                     VLTGNETRDNWIRKDMEIETDSQDVLMSTGLKDLKAHAYPAITYEVDGYVDLEIGDVV
                     RIQDDGYQPPLILTARVIEQVLSKDNPNSNKTKFSNFVEKESQLASDLISDMLRLYDE
                     SIPYEIKLATSNGVAFKNGTGESVLTPSLQKNGKDYEAVYFYKNGDSLIDIGPSLIVK
                     ASDFNHVLNVTVEAYLNEELVASTQISFTDTEDGADGNTPYWHVAYANSADGTVDFSV
                     SDSANKRYIGQYTDYDAIGSNDPKKYKWTDMVGTVVVGTNNLIDGTKSFSGEDWFTSA
                     TLEDENISNYPFTFKKWISGQKVSHAKDIMVEQGVTYTFSAYVKREVAGNLYFYLYDI
                     ADGFITSDTPRETIIKNVDSSLRRFEITFTPTKTGRIRPRFAMVSSEQGSFSSGGFML
                     VRGNKTGDWQESEADKASNLDAKADGAFTVEQLNALAERARIAEAELQSKATLDTVND
                     WVKALQDEIKAREEGQKLSEQKLIDSSNRMIAVQQTIGEMQVRTDFVNKFMSQSEDGL
                     VIGQKDGTSSVRVDNDRISFYSSGKEVAYIAQSVLVIDSGIFTTKLQIGRYRIEQYEL
                     NPDINVVRYVG"
     gene            38854..40857
                     /locus_tag="VBC94_000271"
     CDS             38854..40857
                     /locus_tag="VBC94_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978574.1"
                     /translation="MTTYYSNSDRSYRLTYIVDEVSTSIADNSSQVRFRLYLTSRTNS
                     YAQYNFSGYAWVGGRYDFSAPSALGFNSNYLLIDKTIKVPHDADGSKTVVVAAKLSGP
                     GGYAPGTLNIPDQKFTLTKIPRTSTVAVSSGYFGDTLNVNINKTNDSFTYDVRYNVNG
                     ITGTVASDISGSTTFKTSLDWASTIPNTTSTPATIYVDTKSNGSVIGTSTGIFYLTVP
                     DNIKPKISSLTLSDTNQKASTIVGANNFVQIISNPVVTFNGASGAYGSTIQNFNAEII
                     GKNQSTQSNGGSLGILNFSGKATVKATVTDSRGRVSDPVTTEINVIPYSPPAFSFTVT
                     RAGAKNDQLVVTRNAKISPLIVDGVQKNKMTLTFKTAPLNTTSFTIDTSNASGTYTTV
                     AELINSTATLSGSYGANKSFDVYGLLSDVFSASGGGTPVKQTVSTESFPLSWHKNSVG
                     IGTLPKIDDTGSLNVAGNIYSDGKQIQQKQLALNNGGAFRHDTTDLNSLQDTGFYCVF
                     KGDNRPSGAGPGYLTVVRHETANYAYQHFYDRTNKTIFTRVLENGAWSGWSEYAKKDS
                     LPQSAPAVEDTGWQYIGNGFNYRKIGSMVTIKYDFATNGINQFTVGSMPTNLIPNEMM
                     FAVTAWTVQLNVLNVQVSADGRILWFNPSKWAVNVKGQINWII"
     gene            40869..41261
                     /locus_tag="VBC94_000272"
     CDS             40869..41261
                     /locus_tag="VBC94_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEO6978575.1"
                     /translation="MLEFLNRYPVLLEDKSVKETKAILAFTSSTIKANFEVTLPAEEN
                     DKKFAETLKTCEKLIFEQLYKDKAEAEQFEKINDAIAKSKAQSDKAENMIKLMSATVN
                     DLIKTMADGGKLNDTTLNNASENSSTHI"
     gene            41421..41723
                     /locus_tag="VBC94_000273"
     CDS             41421..41723
                     /locus_tag="VBC94_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978576.1"
                     /translation="MNIDILQIGAASGAILSVVGLWAFVVNPFKTAMQKNEDTMSALK
                     DTIKELAYELKDSQRDREKIHKILDIHEQRLGKTEDDIIVNKEQIKTLFNRRNKYD"
     gene            41716..41943
                     /locus_tag="VBC94_000274"
     CDS             41716..41943
                     /locus_tag="VBC94_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO6978577.1"
                     /translation="MINLKLRLQNKVTLMAILGAIFLLAQQVGIKLPSNIADIANTAV
                     TLLVLLGVVTDPTTKGLSDSEQALNYHEPKK"
     gene            42069..43403
                     /locus_tag="VBC94_000275"
     CDS             42069..43403
                     /locus_tag="VBC94_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEO6978578.1"
                     /translation="MATYQEYKNRSNGNAYDIDGSFSAQCWDGYADYCRFLGVPYANC
                     TNTGYARDIWEQRHENGILNYFDEVETMQAGDVAIFMVVDGVTPYSHVAIFDSDAGGG
                     YGWFFGQNQGGTNGAYNLVKIPYSATYPTAFRPKVFKNAVTVTDNTGLNKGDYFIDVS
                     AYQQADLTATCQQAGTTKTIIKVSESIAWLSDRHQQQANTSDPIGYYHFGRFGGDSAL
                     AQREADLFLSNLPGKKVSYLVIDYEDSASADKQANTNAVIAFMDKIASAGYKPVYYSY
                     KPFTLNNVDYQQIIAKYPNSIWVAGYPDYEVRSEPLWDYFPSMDGVRWWQFTSVGIAG
                     GLDKNVVLLADDNSQVTVPKADKPQEAVSFNQRLDVNTKLDNSNTPYYEATLSTDYYV
                     ESKPNASSSDKEFIKAGTRVRVYEKVNGWSRINASQSDQWVEDKYLANATQV"
     gene            43567..43773
                     /locus_tag="VBC94_000276"
     CDS             43567..43773
                     /locus_tag="VBC94_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6978579.1"
                     /translation="MSLVEEKLKAKVEQASGSLKEGAGKLTGDKELEAKGFVEKTIAK
                     GKELADDAKDAVEEAVDAVKEKLK"
BASE COUNT        15439 a         7144 c         8964 g        12309 t
ORIGIN      
        1 ctaaaattta tcatatgatt ttatgtaatt ctgatataat gaagatttta gctataataa
       61 aatctcttcc actgaatgat tgttggctgt gtgctggcac attgagaaac tttatatgga
      121 ataaactttc tggaataaat gagacattga catcagacat tgatgttgta ttttttgata
      181 aaaatatttc ttatgaagaa acggtggtat tagaacaaca attaaaggat aactatccac
      241 agtacgattg ggaacttaaa aatgaatttt acatgaacac tcattctcct aataccccaa
      301 agtatactag ctctaaagat gctatttcga aatttcctga aaagtgtact gcagtgggag
      361 aaaggttaga tgatagaaat caactcgaac tttacctccc tcttatggtg aagaggaaat
      421 attaaatttc attgttagtc ctacgccata ttttgaagag gatctattac gttataatgt
      481 ttatttgaag cgagtggata agaagaaatg gaataatatt tggcctcgtt taacaatcat
      541 aaaaaaatga taacgttact ggagttggct taacagcttt taaattttta gactgacaaa
      601 tagtatagac ctttaataag gtcgtttatc gataatcata gtgtactgtg ttataatcgt
      661 tatagcataa aaacacgttg cagttggtag tctgcaagcg ttcagtgaac gtcagtaacc
      721 ttccctccag gtcgtccatt gcaatttaat atttaaagga gggggctgta tgaaaatgac
      781 agtctatttt gatggtaatt tttggttggg tttgattgag tacgatgatg atggtgatta
      841 caaagttttt cggtattttt ttggaaaaga acctaaagat gatgatgttt ttaattttat
      901 aaatcataaa cttaatgatt tgataaaaaa atacgagttt gttaaaacgg atatttctct
      961 aaagcgtacg aatgaacaca aaaaaagtcc taaaagaatg caaagagaaa ttaatagaga
     1021 aaaacgtaag ccagtagtat ctacaaaagc gcaattggcg atgaaaacta ttcatatgag
     1081 tataaaaaac gaacgtcaat taagtcagaa atgtaagaaa aatgagttaa gaaagcatcg
     1141 gtaccaatta aaacaagaaa aacgttacca aaagaaaaaa gggcattgat tgtgtcatca
     1201 tataaaaagc ttgagagtat catctcaagc tttttatatg ttaactaata tgagttacta
     1261 aatagtctga taatgctcaa ttaatgtgtt ttgggaaaaa atcatttcct taaaggacac
     1321 cattgtcatg ttgttatcac cttacttttt ttatacttag tatatgttta agaaaggaat
     1381 aagttgtttt gagagatttt gaggagttgt ttgataaagt aaagccaatc gtaatgaagc
     1441 tgagaagaaa ttattttgtt caactttggg aatatgatga ttggattcaa gaaggacgta
     1501 ttgtactttt taggttgctt gaagagcatc cgtatttatt ggataatgaa agcaaactat
     1561 tcatttactt taaaacaaaa ttttcaaatt atttaaacga tgtattaaga caccaagatt
     1621 gtcaaaagcg acaatttaat aaaatgcctt atgaagaaat cagtgaagtt agtcattatg
     1681 ttaagagtaa aggactagtt ttagatgact atattgctta tcgtgatact ttaacaaaag
     1741 tggaagaaac tttgtcagat attgataaag aaaaatttga aaaactgatt aatggtgaac
     1801 gttttgcggg gaagaaaaaa ttcatacgaa atattcaacc ttttttcaat gcttttaaag
     1861 cggattaatc gattttattt ctgcagtaga tagctgcaga atttttttat agggaaagtt
     1921 cgctgatata ttcctgtgaa agcctttgtt ttttgttata atataagatt acctctcaga
     1981 atcattgata tcaaggcttt tttaaagtct aaaaacagac ttactacgtt ccccactacg
     2041 tttgtgttaa atagcgacga gttcggccaa tttgtcagca acattatcct ttgatttttt
     2101 ggataagtga gtatataaat ccatcgtgat tgctatttta gcgtggccta accgctcttg
     2161 tgcaatctta gcgggaatcc cagcctcaaa caacaacgaa gcgtgggtat gtctaaaccc
     2221 gtggggagta atccttttta atccatgctt caatataaat ctgtccagtc tttttctcac
     2281 ggtgtcgcta gcaaaattaa acaatttaaa cgatccgtgt aaaggtttga tactttgctt
     2341 aatatataat ttggctaacg tcatggtctc agcgtccatt gatatggtgc gattgctttt
     2401 tttggtttta ggagattgta cagcccatcc ttcttttgta taagcaatgg tttttgtaat
     2461 gctgatagta ttgttgcgga aatcaaagtc ggtatctttt aaagctatta attcaccaat
     2521 cctaaaaccg ccataactca gcaatctata aatcagacgc atctctagag tttcttcagc
     2581 ttccacaatc ctcaagaaag cattcaattc ctcttttgtg taatattttt tggtgagtgt
     2641 atccatgttt ttgtaagtgc actttgatct caacgtcttg ctcatggggt tattatcaat
     2701 aatacctaag ttgacagcaa atttaaaaat tctgtttgct aaacttagat aaagcctata
     2761 accgctatat cgagataggc aattcacaaa tttttgacaa atcatgacag ttatcttcgt
     2821 catttgcagt tgtcctaaat gtggctttaa aattgtttta tagtagtttt ggttaaccag
     2881 aaatgttgac ggtttgactg tatttcggta ctgctcaaac cacagtttgg ctacatcgtc
     2941 aaatgtagtt ttatcgttgc ttttccaagc gccttgacgc tgaaaatcat caataagttt
     3001 tacctcggct cttttagctt ctctttctgt tttaaaccct tgcctagttg tcctgacttg
     3061 tttaccagtc atgggatcaa caccaaggta tgctcttaag cgataggcag ttgtgccatc
     3121 ttttttagtg tatttttcaa tcattgtttt ttcctctctc tttgcgctgg ggagtgctga
     3181 ttttgagata ggatattggc atcacctcct aaaaatgata taattaggta caagaaaacg
     3241 acctttttaa tggttgtttc ttatactaga ttcgccccac gctcagaccc tcccaaattt
     3301 gagcgtgggg ctttttttat ttccataatc ctgagttata tctcactttg atatctttaa
     3361 tcttgctgtt tgtcccaaaa acaatactag ccgctgctgt ttctccatct tttacaacaa
     3421 cgctatatcc gtcattatct aaattttcgc tgataactga atcaaaagaa atagtttcat
     3481 ctccattgta ggcgaaaata tctcttgtat caaaaccttt tgtttcccca gttgtatttt
     3541 tgaattctat cggcacaatg attttatttt tgaaatctgt tgttgttgct aacattcttg
     3601 ttttgtcatt atttggttta tttatagtta agtaacctcc acccaattta acttcatcac
     3661 caaaagacga tacaacttga tctttattaa gataaaattt aatagctctg acagacaatt
     3721 cttcgtcaaa acgactggta aaatcggata tggttttcct aatacctgac gtcgttttat
     3781 tttcttgagt tttttctaat tttgctattt tcttctcgtt aacaatatta gtctctgcca
     3841 atctgtaaat gacaaaaaac gaaaatccta aaactaatag tagtatagcg tgtgtccaaa
     3901 aataccactt tttataaaaa ggttttttca tatcataaat ctcctaaatt aaatttttaa
     3961 actcatttac taccatcgct tcatcgcaga tggtgtttaa cctatacgct ttcataaatc
     4021 tataaacatt aaaatcttct atgtcgtcaa gttttccaca gactaccctt ctctataaat
     4081 atctaccacc tcaccaatgg ttctgaaatc atctccttca gctggtgtga tgtcagcata
     4141 ctttggatta agtgactgga ggtagtcgtt ctttaatttc ttgacatagt tttcaccgtt
     4201 aacttggaaa ataccaattt tgttaatgtc aacttgatca gccagcttaa taaataaaaa
     4261 gtcgccattt tttatttttg gttccattga gtgaccgaca acaaccgcaa cggtgtcata
     4321 gtttttttca tctggtattt catcagcgta gaagtcaacc tctgtgtcat agtcatcctc
     4381 ttgccaatat ccagtaccag cagacacttt accgggtaca gatagactta cacgttttct
     4441 ggagccgtat tctgccatct tctcttgcaa gtttatcact ttgccttgtt cctcagataa
     4501 aagtgtctca gatatagcca gcaacttatt ctttcgtacg ttgttaagtt ttgaatagtt
     4561 agataagaga attgcttgcc ttggatcaaa gttgactttg gaagttgctc cattagtaga
     4621 gtgttcttca atcaaatctg acttgttaat tgaaaagtat tcagccaaaa gttctatttt
     4681 ccctattcgt ggataagtta tacccttgag ccaatctcta accgtagtat acttcagtcc
     4741 taagtctgca catagtgtat ttctgtctac tctttttcgc tccatataaa atgcaaggtt
     4801 tttagaaaat atctctttgt tctctaattt ttttgaagac atttttgcac ctccttataa
     4861 tgtatatatt acggcaaaaa cgcaaaaaag taaataaaaa aataaaaaaa ccgtaaaaaa
     4921 acgcaaaaaa atacttgaca ttgcggttta accgcagtat aatataatca agcttaagga
     4981 attaagcaaa acgaaaggag gtacagctga tgaagtcgag gctaaacaaa aagcctaaac
     5041 acaaagaact agaagtcgaa atcaaaattc tttggtttaa gcttagaata aaacatctga
     5101 ttactaggta atcggatagg gggtgaaact cccccacccc taataggggt aagtttagtt
     5161 tagcacattg gctgtatctc cgcaagaatg aaaggagaag ttatgagtga gcaaaatgaa
     5221 aagtttatcg gagtgctaaa agatatgcac atgcaaattt tgcgagattt cgtaaacgca
     5281 gaagacccct taacaaggtg cgaacttgct aaagggttcc tcgaaattgg cgactattta
     5341 tcaaaacaat gactcgccac gctcaagagc ttcggctata actttaccat tattgatttg
     5401 ttcttgcttt tcggcttcaa tgaaaggtaa attatgttgc ttaatagctt ctaaagagtc
     5461 ttcgtaaagt ttgattttat cttcgataga caaagtaggg ctagaagaag caacaatagc
     5521 gagagctaaa tcttttgaat tagaaatttt cattagctta tcctcctttc tgctaggata
     5581 agttgattat aacattttta ggaggtacaa aatgaattgg aaaaaactaa tgtttggcga
     5641 tctagaacac acgtttacta gtcgtaatgg caaagaaaaa acaagtattg aatttgaagg
     5701 tggcttattg ccagctctgt tagtgctagg tggtatcact tggctgatcg cttggtttat
     5761 tacaaaataa aaactcccaa aaggagtgga aaggagggaa ggcatggaaa aaattaaata
     5821 cggtgtacta ggttcttcca agatatttca cacaagaaaa tctgctctca ctcatgcaaa
     5881 tcttttagga tatcctcgta gtgcggtttt tttgataatt ccacagggaa tgacgcagaa
     5941 aatgattgat aaggtgaatt aacatatcct gctttattag ctttaaatag ttttttcttt
     6001 ttagctactt taaaagcaac aaagagtcta tctgtgattt cagatgtttg tatgattaaa
     6061 tccatccttg tcatactgtg ggcttttaat actccacaat ctgcctctgg aacctcgata
     6121 aggattgtat tgccatcagg ctgtactgcg gctatagcct ctctgcctgt taaatcgtta
     6181 attatattat tttgcttttg gtaataatgc tgatattttc tgtttttatc aaaaacaatc
     6241 aagtcgaagt agcttacatc aacattagaa ggattgatga ttttaatgtt agctcttaat
     6301 gtaccatttg gattatatat gctttcaccg ttgtctaaaa taacgctcaa aatccaatct
     6361 gaaacaggag cagcaattaa ctcgacttgt aagttatttc ttcggtagtt tgaataagat
     6421 agaaacagag ctattaaagc tatccaattt tttattagat attcacttgt aaacttaaaa
     6481 acacacaata aaaaattaaa aaaattcatt tcaacctcac atttttattt aaattatacc
     6541 acagaaacag aaagggggtg gggaagatgg aagaaatttt gtcaagcata gcaaaaagcc
     6601 ttgaatccat tgcgactgaa ttcaaagctc aaaattctta cagagaagaa atgaaacaaa
     6661 atatggaaca aatggaaaaa ataatattag atatccaatc agatccattt ggactcaaac
     6721 acttaaaaga taaagcgttg tccgaaaaag cttctaagca aaagggatag catcctttat
     6781 tttcttagca aactcaagcc aatattctat cctgccagat aaagtcttgg cttggtcttg
     6841 tttttcaagt tcagcgatag cttcggttgt taacgaaatt agataaagcg tatcgcttgc
     6901 agccgtcgct gaaatatagc catgttttct aagttcaaaa caagtatcta gtacatcttc
     6961 ttcagaccat tcattcataa tgttttcttt gatgaattga atatcttcga aattgcgaga
     7021 cttttcttta gaaattttat ctttacgtct ctcgttgtat ttgacaaaca ttgaacttaa
     7081 aagaaacttg gcatcatttg ttaatttatc catatattca cctcctttct gctcacatta
     7141 tagcagatta gaggtactaa aaacagatag aaagggggtg ggagaatgac gaaaatgacg
     7201 ttgaaagctt taagagcaac aaaaaactgg agtcaagaag aggctgcaag agctttgaaa
     7261 gtttccaaag atacatgggg caactgggaa agaggtaata cagagccaag tgttacaaag
     7321 gcttatcaaa ttgcctatat attcgatata tcattagatg atattatttt tttaccaaac
     7381 attgcggttt aaacgcaaac tcttagaaat agaacaaaac taacacaaca ctagaaagga
     7441 gaaaaatgca ggaaataaca acacaaaatg attttgacta ttcacttgtt gattttgaaa
     7501 ctcaagaatt tttacaagag cgttcaaata ttatctatgg aattcaaagc aaaagcgctt
     7561 atgaaatagg taagcaactt gttaaagctc aagaagtttt ggcaaaaaat agatatggtt
     7621 gttttgaaga gtggtattca agccttggat ttagaaaaac aaaagcttat gagtacatta
     7681 atcattataa tttcattcgt tcgcaaagcg aacaattgaa tatcgaaaca tttgaagaac
     7741 ttccaaaaaa gcttcaatca gaaatgtcaa agccgtctgc caatccagaa ctcaatcaaa
     7801 aagtctttga tggggatatt acaactcata agcaatacaa agagttagaa cgccaactca
     7861 aactagcaga agctgacaaa gaacggttaa aacaacaaaa tgagagacta gccgaacaag
     7921 ccttgagtgc taaagtcgtt gaaaaagaag tcgtaatcga aaaagttcca gatgattatg
     7981 aatctacaaa gcaacttaac cggactttac tagataaaaa taaagagctt tccacaacac
     8041 ttgaagaagc tgaatgggag ctagatagta aaaagcttga gttatcgaca ataaagcttg
     8101 agtcacaacg agctattgaa gtaacaaacc agattcgtca cctcgagggc aaaaaagaaa
     8161 aacttgagaa ccttgttact tcgataagtg agctgtcctc aatcattagt gacgtgcaaa
     8221 acttcttcga tacgaaaatg gcgccacttc gttttaagcc aattatcaac aatgtcaatg
     8281 cacattattc agttacggaa gtaaccaaga tggttaacac agtccaaact tggtgcgatg
     8341 aaatgtacaa aattattcca tctggaaata gaaaaacagt agaagaggta ataatcaatg
     8401 agtaaagaat tatcagaaat gaatgaaaaa gacgctcttg aagttgtcaa aggaatgacg
     8461 attgaagata tgatgattga agtcttatct tctcaaaaac aagtaaaagc ttctcaagaa
     8521 gccattaaga aagatgttga agaggttcgt tctttagcaa ttgaaattga taaaaaagtc
     8581 catattgatg atgtggaagc tagtgaaatt aaaagcatta tcagtcgtca atcctatgaa
     8641 ttcgccaaag aatattttga taatagaaaa gttgttccaa gcgcaaatct attcgctagc
     8701 aagaaaggac agtttattcg tgtacagcat tcacgattaa aacatcattt caatgtgacg
     8761 aagtacactc atatcaaaca tacagaagca gaaaaagcta tcagttattt aaaatcacta
     8821 tcttatggtt tattcacacc atttgaaact cgtgaaacac caaaacaaaa agagattatc
     8881 gaactggaaa ataaaaataa cggtgatgat gtcgcttaga actagaaagg aaacaacatg
     8941 aacgaaaaaa ttacagatga acaacttaac aaatacttag aaatcagaac tgaacttggg
     9001 gaagaaaact ttaaatttat taacgcttgc tatgatgaag ttttgcacct aaaaaaagag
     9061 gaagcgacaa aaaatattaa gtttgaaatt acagattttt caaaatttcg tgattttgct
     9121 aaaaaatagc cctcagaatt aactgagagc ttctagttac atgatttctc cacgcatacg
     9181 tgccatacgt ttgtcgtgtt catgcacagt ttcagagaga tttttgccag ataaatgttt
     9241 attgagtatc tctgccaaag ctactgataa tttatctgat tcagacacgt cgtgtctatc
     9301 caacgtctct ttaattcttt gtgaactaat cataccttat cctccttcct ataggatagc
     9361 tacattatat aacagaaagg tacaaaacac aatatgttgt gtatcttaga cgattaaaaa
     9421 cacaatatgt tgatttttaa aagtattatg aagaaaaaat tgaacaaaat tctcattgat
     9481 aaaggaatga gtaaaaaaga gttatcggag aaaacaggga ttagctacaa caccattatg
     9541 aatatcggta aaagagatat ttcgtttaac aaaatgaaaa aaatcgccga tgtcttagac
     9601 attagcctaa acgaattcag ataaaacaaa aaaagccact gcgggaacag tgactaacca
     9661 aaataattta actaattata ccacgaaaga ggtgataaat ccatgcaatc gcaacttact
     9721 tacgatctac tcaaaaagca gatagcagaa gaactttttg atgagtttaa ggagctcata
     9781 caagaaaaag atttagcgaa tcggtgggtc aaccaaacag cactcgtcaa tgaatacggc
     9841 tactcatggc aaactataaa acgcatggag agctatggcc ttaaatcttt taaaaacggt
     9901 aaagacaaaa tgtactgtct tgctgatgtt aacgaaatta aacacttaat gaaacaataa
     9961 gcgctgggga gtgctagagg agaaacaaca tgaaactatt taattttatt tttgcaaaac
    10021 caaaaaaaca ggaagaaaaa ccaaaatgga cgattgaaac acatggctgg gaagctaatg
    10081 cacgacgtta cgacaatatg attgaacata ataatagagg gcgtacatgt taaaagaagt
    10141 attactagaa attgaattct tacgtgatga aaatagacga ctaactaatg agttgctgga
    10201 ggtattaaat gaataacgat tggaaaaaaa gatacattaa caatcaaata gactttttca
    10261 atgacatcaa gacatgtgtc aaagaaagat ggccaaatat gaaggggaat gatgtcgata
    10321 aaatttcgat agcgatttac agaaattggc ccgtaagtga catcaatagc acactagaag
    10381 tgatagaaac ggatttggaa aaattatgaa atgttatgtc aataaacaaa aaaaactagc
    10441 aatagacatg aattataaag ataaattcgg aaaatttagt tctgactcta ttcagatttt
    10501 agaaggtaaa ttaactgatt ctattcaaat tgatgtcgaa aatgctatga aagaaattat
    10561 cgataagtat agtcaattgt ttgatacgcc tataatcgat gatttattca cagaaaaaga
    10621 aaaacaatta aagcaatctt atgatgtcga aactacattg acagaaatgt tcgaggtgga
    10681 atatgaagat aactaaagca acagagatta caaagactca taattggcgc atattaatct
    10741 atggcaaacc cggaaacggg aaaacttact taattaatta tttaaaagga aagacactga
    10801 ttttagacat ggaccattca tctaaaacga ttgccggaaa cgaaaacatt gatattatcc
    10861 aatttgacag gacgcaccct agcgacttta tgactgaatt cttgacagaa ttaccagaac
    10921 ttatcaaaga atatgacaat ttagtcattg ataatatcac aagttggcaa tcagactggt
    10981 ttatcgaaca aggtcgcaag tctaaaaatg gaatcacaaa cgaattgcaa caatacaaca
    11041 tgtggaccaa ttactattta cgagtattga ctacaattta cagccaacct attaatattt
    11101 ttgtgactgc ttgggaatca acgcaagatt taacgctcga gagcggacaa atgattacac
    11161 aatacgtccc agatatccgc aaacaagtcc taagccaaac gctaggttta accgatgttg
    11221 tcggacgtat tcaagtaaat ccaaaaactg gtggacacgg gatccttttg gaaggaagcg
    11281 atggactgta cgcaaaaaat aggcttgata ataggaccgt ttgtaaggcg gaagagttgt
    11341 ttaatttcga agggagtgat gcgtaacggt ataccattta cacgaatatc aaacagaact
    11401 tataaatgag gcaagaagac atattttaaa gcataatgtg atgattgtta gtccacctgg
    11461 aagtggtaag tcagtagtca tttctgatat tgctaagtca gcgactcaaa aaaacggata
    11521 cgttttattc ttggtccatc gcaaagaatt aattgaccaa atcactaata gttttaaatt
    11581 ccatggaatt gacatgaata aagtggattt aatgacagtt ggtaaagcta aaaaccgctt
    11641 ggataaatta acaaaaccaa ccttaattat cacagatgag ggtcatcatg ggaaagctag
    11701 tacctaccaa atgatctatg aatacttttc agacgtgccg aggattggtt ttactgctac
    11761 accttggcgt ctatcaggcg acggttttac agacacttac gatgttatgg ttcttggtaa
    11821 aacggtcgag tggcttatca ataataataa acttgcacca tatgattatt atagtgttct
    11881 atcaattgat actgcgaaat taaaagtaca aaacggagat tactccaata aatcgattga
    11941 cgaatcattt ggtaaaaaga tttttggtga tgtagttcaa gagtatataa aaaaagcgaa
    12001 cggtcagaaa gctattttat acgctcactc ggtagaagca tcgcaggcat ttgctaaaga
    12061 attccaatct atgggaatta atgcaataca cgcagatgca aaaacgccta aagccaaacg
    12121 ggataaaagc atgaaagatt tccgtgacgg taaaatacaa gttatctgta acgttgattt
    12181 gatatcagaa ggtttcgatg tcccagattg tacagtaact attctttgta gacctacaaa
    12241 atcattagta ttatttttac aacagtctat gcggtcgatg agatatcaac ctaataaaaa
    12301 agctatcatt ttagatcacg taggaaactg gaatattcac ggcttacctg acacaccgca
    12361 tcattgggag aattatttcc gaggagggtg gaagaagaag tcgaataaaa ctaacacggt
    12421 acacgcaaaa gaatgtcctg tgtgttcagc tttgtggcca cttagtcaac agctctgtga
    12481 attatgcaat catgattttg gattgaaaga aaaacaagag aaagaacgca tagaggcaga
    12541 acttgaactc ataaaacgtg agcggtttag aatcaaacaa cttgctaata agaagtttgg
    12601 taaagattta aaaacaaact gggaaattgc ccaagctaga gttaaagacg ctggcaaagg
    12661 aaaaccatta tataaactta tctatttcta cttaaaaact gattgggtag aaacaaacgt
    12721 taatgaactt gccgaagtaa caggcaagtc agaaaaagaa atatatagcg cctacaattg
    12781 gctaaaaaag aaattaagag gataaaaaca tggcaggatt tacaacagat ttttcagaag
    12841 ttaaagaaca cgcagaattc aaagaacaac catacgaaat gattgtttat gatgcatatg
    12901 aagcagtaaa tgaccgtaac ggcaaaaaac gtgttgttat tgactatgtt gttcgaaacg
    12961 atatcaagca agaaatgcaa aactaccact tatgggatga gcaatatccc aactcacaaa
    13021 ctgggaagta tcatatcggc attttaatgg gtaaagcaaa agcacttggt atcaaagagg
    13081 ggcaacacta cgatagtttt gaagcgtttt taaacgactt caaaggacgc actgcaaaag
    13141 taaccgttaa acttgacgaa tataacggaa ataaataccc gaaagttcgt tacgcaaatc
    13201 aaagcgatgt gcctgaaagc caccacatat ggaaagaaaa aacaactgga tttacacaag
    13261 ctgagattga agaagacgat ctaccgttct aatttagact gggggataaa tgacatcaaa
    13321 tgaatttatt gaagctttat caaaattgac caccgaaacc gattggggag acccaatctt
    13381 cggcgagtca gtgcttaaag ctgaattacg aaaacactta tttaaaattg tcccaattga
    13441 tcacaatggg tatatccaca aactgtttta ttcagaaatg gttaaagatg aagatgtcat
    13501 gtattttgtg tcagatggac gaaaaactta tcgcttttta tttggagata caattctaaa
    13561 gactgataaa caaggcaatg aatatctcac atactccgtt gaaaataatt ttccgccatt
    13621 tgcaaaacta gttatcgact acattctagg tacttacacg ttttttgaga ataaacttta
    13681 tgacattcga tataagcaat ttaaattgat tgatgatttt acacttcaga ctaagtatgg
    13741 tttcaaagac tctggtcaca ttttagagat actacaaggt atccacaaaa cattaaacat
    13801 ccaaccaatc aattatattg aaccatatca gattgcttgc aaggatttca taatcgacct
    13861 tgaaaattct gaaatcatta accaaccgcc tttgcaaaac gtatcttatt ttaagtatta
    13921 cgaagtagac tacaaaacag caataaacag taagtctatt gcagaagaat atcttgaata
    13981 cgttattgca gatagcaatt cgttaaacaa tgcaatacta caatcttatt ttatcgccca
    14041 agtggcgtgt ggtgttagac ctaaaaccaa cttcttcatc tcaaaatctg gagtaaggac
    14101 tggtaaaggg ttaaggcata tagctttatc tggtctattc aataagattg atgttgagct
    14161 agatacatta aaaagtaatg gatttgaagc gttacaggcg tgggcgatgt tctcgggtgg
    14221 agaaatggct ctagcgacgg aacagggaga tattcaaggc aatgcaatgg agcgtgtact
    14281 taaaattatt gcaacagaga aaacacacgt tgcacgagca attgggcaaa atcagtcaat
    14341 tgttaattta acgagtgttc tgtgtattga cacaaaccgt actgttgcgc tgtcggatga
    14401 aatgaatgga cgaaaggttt taattcagtt taaagataga ccaaaaactg aaactgatta
    14461 tgaaagagag agcgtattca ggaaatattg gctagcattt actgatcgtg ataaaaatcc
    14521 aaaaatcgat ggttgtatcg gttttctgtt aaactcactc gagcgtttcc aaaaaattgg
    14581 taaatggtat caatggaaag atgttgaagt atttaatgat atcgatttag acgaatttca
    14641 agttgcttta ataaatgcat tacaagaagt cgattttgta cagcgaactg ataacaaaga
    14701 agttattgac ttatcattac aagtttacgg aaaaagcaat catgcagtaa gtaaagccat
    14761 atctgaaatt ggtgtacgta gcaggtcgaa gaaagttaac gggaaaacag tcagagggta
    14821 tgaaatcgaa aataaaacac gttttgataa atatatcctt taaaaaggta acgcatggtc
    14881 acgcagagtt acgcaaaatt ttccatgttg cgtaacccac tcaatccctt tagtaccaac
    14941 cgatttcagc ctttattcaa gaaaggttac gcagtaacaa acaataagtc taattaatga
    15001 tttattaatt atttatttat atataggggt agggtagggt gattttgcgt tactgcgtaa
    15061 ccatccttct ctaatgctct atgtatcaac gttttagagg ttacgtagta aaaaaggagg
    15121 tgcgttacct tatgtaccac acaacagcac tttcattctt aaaaaaagga tatcaggtta
    15181 taccgcttag aaaagatact ggaacgccaa tgattaagtt taaagatatc caaataacgg
    15241 aagaagtgat caaaaataca aactggttta attgtgatta tgctttatta atgcgtggca
    15301 tttggtgtat tgatattgat actcatgaca tggacgagaa gttagctaaa gagttataca
    15361 taatgataaa aaagatgggg attgatttat tatctgtatt atcgactgat aagtatgaca
    15421 atggactaga tggttattcg tcaattatta ggcatgagta taaaaacgaa ttaatcagta
    15481 attttaaaaa tacattcgca gaattaactg caagtggtgg tatgcacata ctctttaaaa
    15541 aacgcgatga tattaattac acgcaaaaaa taggagtaat gcctggtgtt gacataaaag
    15601 cgaatgataa taattttgtc aaaatattcc catctgacgg acgtgaagtt ttacaagcag
    15661 ttaaaacatt accttattat gatggaaaat ttgaagagga agtatttaaa ccaaaacaag
    15721 aaggtataac aacctatttt ggatgttctg tcacatatac atcaaacgga agtcacgaag
    15781 gccgagaggc ttatgaacgt gtagcaactg gaacatcata taacaggaat gatgatttgt
    15841 ttaaaggagc gtgttgggcg tttgaaaatg gtatcgatat tgatgattta acatcaatta
    15901 ttgggacggt taaaggcaga gacgtattta cgagagagga gtttgaatta acaattgaat
    15961 cagcgaaacg aaaagtcagc tacgtcacta tcagaacatg atatccaaaa ccttatccga
    16021 atggaattat cacaagcggg gtatatggta tttcgagcaa atgtcggaaa ggtgaaaaca
    16081 gcagatggta gattttttga tacaggttta ccaaaaggtt tttgtgattt gtttggattc
    16141 aaaccaaacg gacaaatatt ttttatcgaa gttaaaaatg aaacaggtcg gataagacca
    16201 gaacagaaaa actttatgga ggttatggca tcgaaagggg cgttagccgg agtggcgcgt
    16261 tcggtggaag atgctttgaa aatagtaaat ggatatcatt aataaattta aaaagttaga
    16321 tcattacaag gaaattaagc gattgatgta cgcagcaatg actttctgag gaggtatagt
    16381 atggcagata aaataaacgc agagagtatg caagctgcat acaacgaaaa ttatcaaatg
    16441 tttttagcta aaaatgcaga ttatgggaac tcgttcgaaa agtctttaaa tgacttcgga
    16501 tttattgctg gtatcgtacg tataagcgat aaatacaata gactatataa tcttataagc
    16561 agtgataaaa acgtttcaga aagcctgtca gacacgttaa atgacatggc taattattgc
    16621 atgatgttag cggtttggct agagaaaacg gagaatgcac atgacacacg tagttagggt
    16681 ttacgatcac attggcggac gagtgttgcc tactatttat aaagataaag agtttaagac
    16741 taaagacgaa gctattgctt accgtgatag cttaatcgct aaaagtgacg cagagtattt
    16801 ttggagagac gagttatgat accgaaattt agagcgttta ataaagagac ccaaaaaatg
    16861 tatagcattg atggctttaa atcaagtgaa cgcaaaatat acagatgcag cttagcagat
    16921 gatgagtttc gctctggtcg cttagagacg tttcattttg tcgaggataa ccttgacgat
    16981 tatattctca tgcaatcaac aggaatgttt gataaaaatg gcgttgagat ttgggaaggt
    17041 gatatagttt taacaacacg tctcatagat tacacatata aaaattttaa aggtgtagta
    17101 aaaatgttag aaggtcgctg gttaattgac acaggtaaag atgcggttgg tttgtggact
    17161 gaagttgatg agaacgaagc aatcggaaat atacacgaaa atccagaatt gctagagagg
    17221 ttagacggat gacaaaagaa gaagtaattg catttctgac agaacagcgt gatttgcggc
    17281 ttatcggata tgagtgggga aaagacaatc tgtccgattt tgagagatgg cagttagcgc
    17341 aagcgaatat gtttttagat gttattgagt ggatagagga ggtgacgaaa tgactgaaca
    17401 acagatgatt gattgcttgc tttatgagtt agcaaaaaaa gacaaattga acattagacg
    17461 aaacaatatc ataacgtttt tatcgattgt gctgatggct atatctattt taaacgtcgc
    17521 actgcaagac cactacaagt cgcaaattac agaattacgg acacagttaa gcaggacaca
    17581 aaagcagctt aaacgtgcta gtgatgatag agctagacag acaaaacgga ttgctgagct
    17641 gactggaaat gggggatagg gatgtcagac ataaaaattt tagatgcttg ttgcggaagc
    17701 cgcctatttt ggtttgataa aaatgaagaa cacaccactt ttatggatat tagacaagaa
    17761 acatttgata ttcacggaaa acatgtaaat gttaaacctg atgttttggg agatttcaga
    17821 aatatgccat ttgaaaataa tatatttaac ttagttgtct tcgacccacc acacttaaaa
    17881 tatgttggcc aaaagtcaat tatgaaagcg caatacgggc aacttgataa agacaattgg
    17941 aaagaagata tttcaaaagg atttgaagaa tgcatgagag ttctaaaagt aggtggaact
    18001 ttagttttta aatggtctga ttgccagata aatgtaaaag aagttttatc agcaattccg
    18061 tttacaccac tttttgggca gcgacgagga acaactcact ggatgacatt tgtaaagttt
    18121 gcagaattga ctgggaatgg agaatgaaat gattaaagta tctaagaaag aagggaaaag
    18181 cgtgcatatt gaaaataatg gagataaagc aatagttttt gtcaacaatg gcaatgacgt
    18241 tactatagtc agcacagaac aaagcatgac tgtaaataat aagcgaggta tcaaatgaac
    18301 attgaagaag cgaagaaatt gatagacaaa cagtctattg gtaaaggtgg tgtcggcgac
    18361 attccagtag tgaaaacaca tattgtaaaa gtattactcg accaactcat tcaaccaaaa
    18421 ccagaagtgc cacagtttgt ggctgattgg tacgagaagc ataaagatag cttagagtgc
    18481 gatttatatt tatatcacat gtcgatatac gatgaagaag tcgaaaaaga tgacttctat
    18541 tactggatgc aaacatcaaa aaatccagtc tacacactta ttaatatgca ccaattcggc
    18601 tacacagttg aaaaagagaa gctgtatacg gttgagatac caaatccgaa tgaaagacag
    18661 ttaagttttg tgctgatgag acagcttagc ggaaatgtaa gtatcaaagt tatgcataga
    18721 gataacttag acttactaaa gacagataat gatttacaac taacagaatc cgaaatccgc
    18781 aaagatttcg actgggcgtg gcagtttaga gaagaggtgg cggaatgaaa gaaaaaacaa
    18841 tttttatatc aaaagaatat gcaaatgact ttaacaatga caaatataat ttgtcctctg
    18901 gctattattt tagaaatggt aaaaaacatg atattgctat tgttaaatat ggtgaaaaag
    18961 attatttaaa aaatactgat ttagcatatg ttgtatgcga taaaatcgtt gatgcagact
    19021 ctataggctt cgtttatcat ggtgaatatg aaacttggca ttttaaacta ttaaacacag
    19081 aagcaaatta aagtcccacg caagcgccta agagcctgca atggctctgt gggtctacga
    19141 gctggaatac tcgttaaact taccctagaa gctttctgta agtattcagc tacgtagcgt
    19201 ggaataatcg ttacgtagtt atagagcgaa atttttagaa agggaaatat cctccgacat
    19261 tttttcataa aaatctaaag tctgttatcg ctcacagatg attatacaag cgcaatgctg
    19321 caaataaagt gctgacgcaa aactaaaaat ttaatactcg acaatttaac aacaaaaata
    19381 agtcagcaga ggaaggaaag gagaacaata aaaaagccgg ctcacgcaag ccagctccga
    19441 tatatttgtc tactaatatt atatcatacg aggagctttc atgacgtttt ttccagaaat
    19501 taatatacaa aaaactaaat caaacgctaa gcgaaaacta agagagtatc cacgctggcg
    19561 taggatcgct aatgatgtag atactcaaaa agtgacagcc acttattcct ttgaacctag
    19621 acaatcacat ggaaccccta gtaagccggt tgaacgctta gcactcaatc gtgtgtcagc
    19681 ggaacaagaa ttagaagcaa ttgagcaatc ggttagtatg atactagagc cagaaaagcg
    19741 caggattttg tatgacaaat acttatctcc ttacaagaat gcagataagg ttatttatac
    19801 agaattatgt atgtcagaga gcttttatta tgacacgcta gatgctgcat tattagcttt
    19861 tgcagagctt tatagggagg gctctttgat tgtagagcaa ggagtttttg actagttttt
    19921 atacagtaat acaatagttt atacataaaa atatgtgtta atatagtatt atcaaaatag
    19981 caagaagaga taatcattta ccaacaggct atttatttag tcgtcaactt taactactat
    20041 cgaacttgct attttgtagc taaaaggcga gatagggtgt tgagacgtag ctcagttggg
    20101 ggagcgatat gactataaag ggtctggaac gtacgcaggt tcgaatcctg ccgtctcaat
    20161 cagcgtcaga ggagacgcga cccagtatcc cgaaaggcag tcacgcactg atatactggt
    20221 ttttacttgg ttgatagtca tattcctacc aatgacgatt ggtaaaaaag atacatagat
    20281 cggctgtata gtctacgtgt acgtattcac aacagattga ctcatttgat taaccacaag
    20341 taaaacaagg gtcgcaacct tgcttgtggt tagtagggac aatggttgta agtcggttcg
    20401 attccgactg ttcctgttcg ataaatagaa gtgtcccaga atggggtagg caataggctt
    20461 agcattcatt tgctatttat ctatggttaa ccaattagtc atcacattgt ggtggctttt
    20521 tattatggag gttagggtat gaggccacag aagttaacca tcgctggtgg tagacgtaca
    20581 acggttgatt atgatgatag gtcagcagag tatcgcgact ataatcgcaa tcgctggaag
    20641 tacgataagc aagtcaaaca gttttataac tctaagttgt ggagagagac aagtaagcaa
    20701 gtcttgttgc aaaacgatta tgtctgtgct atgtgcggtg gagaagcgac tatgactgac
    20761 cacatagtct cagttaagaa agattggaac agaagattag attggaataa cttgcaagca
    20821 agctgtaaag cgtgtaacga tagtaaagcg ataagagaaa gatgtaaaaa taattaattg
    20881 gaaaacgtgc gtaaaattaa caacagcacg ttaatgttcg gaaaataccc cgcttgtttt
    20941 tgaacagggc tatatcgttc gtgtttttga gaacgcgccc ttttccgtgc aaaatattcc
    21001 ctttttgaaa tttttgaact gtcaattttc gtgtaaagga ggtaactttg ggaagaaaat
    21061 taaaggtggt tgaaacaaca aaaaaacacc tcacaaaaga agaaaaaata gcaagagaaa
    21121 ccgcgcagaa taaggcttct gacggacttg ataaattaca aataacgccc cctcggcatc
    21181 tcaatgaggt ggctagagcg gaatatcgta gaatcataaa tgacctccag accctacccg
    21241 taagaaacct cgatagaggc cttctagaac tctattgttc ttggtatgca atctataaag
    21301 agacaaccaa gaaattagat gaaattggtt attttacaaa cgacccggat aaagggttga
    21361 ttccaagtcc gctcatttta acattggaaa aagctactgc gaatatcaga agtagcgcta
    21421 gtcaattagg gctgacagta gatagtcgta tgaagatgtt tatacctaaa gaagaagaaa
    21481 aacaaaaaag tatatttgat aaatttggcg gttagttaga aaggaggtca tgacaataga
    21541 atacgattat tcagcaatta gcgacattta taaagatgac actttttatt atgcaaaaaa
    21601 gattgttgat gaagagatta aagcaagcaa gaaagtgttt aaggcttgtt taagacattt
    21661 gaatgacctc aaacgtatag aagatgaaga ttttaaattc atctacttgc cacaaaaagc
    21721 atctgatcca attaatttca ttgagatttt accagacgtc aaaacaggaa aaccttatcc
    21781 attagcaatg ttccaaaaat ttattattgg gaatttatat ggatggcgga agaaaacaga
    21841 ccattcctta agacgtttta gaaaagctat gatttctgtt gctcgtaaaa atggtaaaac
    21901 cattcttata gctggtatat tgctttatga atttttgttt ggtcacaatc cgtctatgag
    21961 taggcaattg ttttgtacag cgaacgaccg cacgcaagct aaaatagctt gggatatggc
    22021 aaaaaagcag ttagcttcct taagagcaaa ggatgccgat gtcagaaaag ctacaaagat
    22081 tgtccgtgat gaactaaaaa acttacatga tgaatcttat ataagggcgc ttagtcgtga
    22141 tactggcgca gttgatggat ttgaaccgta cgttggagtg ttggatgagt tcgcagcgtc
    22201 aaagacaaac gaaatgttag aactattgga atctggtcaa ggacagttgg ataatccgtt
    22261 tatcttaatc atttcaacgg ctggtatgga tttgaatgtt ccgatgcaca caattgagta
    22321 tccatacatt actaaaatac tagacggaga aatcacagac gagggctatt ttggctatgt
    22381 cgcagagcaa gacaacgagg aggaaattaa agatgaaacg aattggataa aatctaatcc
    22441 aattctcgaa gtcgatactc tacatgataa gttgatggac tatctaagaa ctcgtcgtaa
    22501 ggtatctcta gagactggag aagtcaacaa agtgttgatc aaaaacttca atatgtggcg
    22561 tcaatccagc gaagaatcat atatagataa acagtcgtgg gagcttgcta agattgataa
    22621 gccagacaca tacaagcgta gggtttggct aggtgttgac gttgggcgtg taagtgactt
    22681 gtttgccatt agtcctgttg ttatgatgga tgattattgg tatgttgata gtttttcatt
    22741 tgtagctaca aagtatggct taactgccaa agaaaagcga gatggtgtat cttatagcaa
    22801 tctagaacgt caaggatatt gcgaaataac aacccttgag agcggggtta tagatgatga
    22861 acgggttttg gaaaaaatag aggagttaat ctatataaac gaatgggaag tacatgggat
    22921 ttgctttgac ccataccaat tcggaacact acttacaatg attgaaaaaa gacatccgga
    22981 atggcctcta atagaagttt cgcaaacgac aatggtgtta aacatgccga caaaacaatt
    23041 tcgtgacgac cttaaaaaag gcaaaataaa gcattctggc aatccactat tgaccatggc
    23101 tgctaacaac gcttatatta aaaccgataa caatggcatg aggattgaca agaacaagaa
    23161 tagcaacaag attgacccgc tagacgcagt tcttgacggt tatgctgtat gttacctaga
    23221 accatttgac ggttctggct actggacaag cgagaaaatt ttaggaggag agacgctatt
    23281 ttgattggtt ttattttaaa aaacatacat acattgatct tgctggtagg tttgggactg
    23341 ttgatatacg gtcttttttt atttggtgat aaggttggtt ttattgccag cggaattata
    23401 ttagttattt tagctatcta tgtagatagt gtagggggaa gaaaatgaac aaacgtatta
    23461 agaaaaaacg caaactagaa cactatatcg cttcactcat cgcagaaaac gttatgtttt
    23521 caaaggaatt aatcaaacaa catgaacgga ttgaacaact tgaaaaaatt gtagagcata
    23581 acgcacaagc gactaacaat gaacttagtc gcatcaaaaa acactcaaag aaaaaatgga
    23641 aaaataacgt caccttacct atagaaagga ggtgagaatc tatgagtttt tttcaacctt
    23701 tgggaacttc aaaggtatcg tatgacgatt atatagcgtc agtcttagct ggtgacgtct
    23761 cacagcagta tctaggtgta tcagccttaa aaaatagcga tgtcttgaca gctacgtcta
    23821 ttatagctgg tgatatagct cgttttccgc tcgttaaaaa ggatataaac ggggatatta
    23881 tccacgatga ggatattaac tacctattaa atgtaaagtc cacaaacaac gcaagtgcta
    23941 ggacatggaa gtttgccatg gctgttaatg caattttaac aggtaattcg ttttctcgta
    24001 tcttgcgaga ccctaaaact ggtcaagctt tacaatttca gttttataga ccgtctgaaa
    24061 ctactgttga agaaacagaa aaccacgaaa tcatctatac atttacggat actttgactg
    24121 ctaaagagat aaaatgtttt gcacacgatg ttattcactg gaaattcttc agccatgaca
    24181 caatactcgg aaggtctccg ctattatcgc taggcgatga gatagactta caaacgggtg
    24241 gtatcaatac cttaattaaa ttcttcaaag atggtttttc tggtggtatc ttaactatga
    24301 aaggtgctca attaagtgga gaagcgcgtc aaagagcaag acaagacttt gagaaaatgc
    24361 gtgagggatc tgttggaggg agtccactag tttttgacag cacaatggaa tatacgccac
    24421 ttgaaattga tactaacgta ttgcaattaa tcacaagtaa taactttagt acggctcaaa
    24481 tagcaaaagc gttgcgcgtg ccaagttata agttgggcgt aaacagtcct aatcagtctg
    24541 tagcgcaatt aatggaagat tacgtaacaa atgacttgcc attctatttt gacgctataa
    24601 cgagcgaaat agggcttaaa acgcttaacg atggagacag gcgactgtat catgttgaat
    24661 tcgatacacg aagcgtcacg ggtcgcaatg tcgatgaaat tgtcaaattg gttaataacc
    24721 aaattttgac acctaatcaa ggattggttg aacttggtaa gcaaaaatca aaagacccta
    24781 acatggatag gtaccagtct agtctcaatt acgtgttctt ggataaaaaa gaagaatatc
    24841 aagatcaagt tggagtcaaa gggaaaaggg gtgaggtaaa tgccaaagaa aataaacctt
    24901 aaaggtccac taattgctaa taattcacaa gaagtctatg actattatgg catggaggct
    24961 gttagtgcta aaagcattat tgacaaattg ccagaagata atagcgacat tatcttggaa
    25021 gttaattcaa atggtggtct cgttactgtt ggtagcgaaa tatataccgc tctgcgaaat
    25081 tacaaaggga aagtaaccgc agaaatcaca ggaatggctg cgagtgcagc gtctgttgca
    25141 gtgatggggg ctgataaagt cgtcatgagc ccaacggctc aaatgatggt acataaagct
    25201 ttgtttaatt gggtttctgg taatagcgac gacttggaca aagcttctag cgctcttaaa
    25261 tcaagcgata aagccattgt taatgcttat gttgcaaaaa caggtaaatc agaagatgaa
    25321 atcatgcaat taatgcggaa tgaaactttt atgtcagcac aagatgctgt tgagagcgga
    25381 tttgcagatg aagtgatgtc gtttgaagct atagcaagca ttgacaatca aatgttgcca
    25441 caagcggtta ttgatgacta ttacgcaagc agaagcaaac gtaagcaaga gattaataac
    25501 atgttgctag aaatcgaaaa agaagaaatt ttacaagggc tataagctct ttttattgga
    25561 ggaagaaaat gtttgacgaa aaaatcgaag aaattaaagc ggatatcact gaattaaaca
    25621 aagtgattgc tgctaaaacc gcagaagtca aagctgcttt agaatcagat gacttagaaa
    25681 ctgctcgttc tgttaaagca gaggtagaag ctactaaagc gaaattagtt gaatcagaaa
    25741 acgacttgaa attatatgag tctagcgttg aagttggtgg tgctgaaaat attggaggaa
    25801 aagaagtgac acaagaggaa aaaacatatc gcgaaagtgt gaatgatttc attcgttcga
    25861 aaggtaaaat cgtaaacgat tctttacgtt ttgaaggcaa agatgaggta cttatgccta
    25921 ttaatgagac aacccccgta gaacctaaaa aagacggtgt taagaaaaca gatgtaaaac
    25981 ctgtctctag tgaagaaatc ctttacacac cagctcgtga agttaagaca gttgttgatt
    26041 tgaaacaatt cacaagcatc caccaagcta aaaaagcatc tgggaaatgg ccagttttgc
    26101 aacgtgcgac tgaaaaaatg gttagcgttg aagaattaga aaaaaatcca aaactaggta
    26161 agccacaatt cacaaacgtc gcatgggaaa ttaaaactta ccgcggagct atcccgttgt
    26221 ctcaagaatc aattgacgat gcagacgtag acttggttgg tattgttgct gagacaatcg
    26281 gtcaaatgaa agttaataca acaaacgacg ctatcgcaac agttctcaag gaatttgaag
    26341 ctaagacagt aaaaaacttg gatgaaatca agaagcttct caacgttgaa ttagatcctg
    26401 cttacaatgt gtcatttatt gtatctcaaa gcttttatca aacaatggat acattaaaag
    26461 ataagaatgg tcgttaccta cttcaagatt caatcacttc tgtttctggg aaagtattcc
    26521 tcggaaaacc tgtttttgtg ctagctgatg aggttttagg taaagataca gcatttgtcg
    26581 gagatttcaa acgtggtgta ttattcgccg accgcaaaga ccttggactt cgttgggcag
    26641 ataacgaaat ctacggtcaa tacttgcaag cagttcttcg ctttggcgtt tcaaaagttg
    26701 atgacaaagc tggctactat gtgacattca aaccagaaca attgccctca taaggccact
    26761 cagggagtgg cgcaaccaga agtaagcagt gctacagttg ttggtaaacc aaccaaagac
    26821 agcaccattg aagatattaa aaaatatcta acaagccaaa atattgactt tagcggtaag
    26881 acgctaaaag cagacttgtt aaaactggct ggcgttgaag aggtatagtt atgactgtat
    26941 ctaaagagct gctagatagt gtgaaactct attgtaagat agattttgat tttgaagatg
    27001 acattattga ggaaatgatt gcatcagcac aagaacaaat atgttttgca atagaagaag
    27061 gctctactcc agaaatgttt aaaaactacg ctaagttttc cttagcggtt aaaaaacagg
    27121 taaaggaaga gtatgatcat cgtgggttat cggcggatac taatcgttat ccgcttgcca
    27181 acggtgtttt gaacattatc catcaattga gattgagggg tgatggggcg tgattacacg
    27241 gaaaatgaat gttagaatag ccatttttag ccaaactggt ggtcagaatg aagatggtga
    27301 agttgtatct gctatcagga aagacttgta tacctgctgg gcagaggtgt ctaaaactca
    27361 attgagagat tttaattatc aatcaagata tcagaatgct agcaatctcc caacaaataa
    27421 agatacaaaa gtatttttaa ttagatacaa tcctaagttg tctattgata atacgatgtt
    27481 tattgagcat aacaaacata tctacaaaat agataaaatt gaatttgatg aatccggcaa
    27541 ggaaatgata atattgagtg gggttagcat atcatgacaa aagggcttga tgaaattttg
    27601 gctaatatta ctaagttaga agtaaaatcg cccaaaattg ccaaagaagc ggtaacacag
    27661 gttgctgaag aatttgaaaa agcacttact aagaatacgc cagtcgatta ttctgtgcat
    27721 gacacaaaac tcaaatatga tactgcaatt agtggattca aaggtgctaa tgtaggtata
    27781 acgtcaaaaa aaattggata tggacgaaaa acgggctggc gtgctcatta tccaaacaat
    27841 gggacgattt accaaaaagg tcaagacttt aaagaaaaaa ccatcaacga aatgacccct
    27901 agagcaaaag aaatttatgc taaaaaaatc aaggagggac tagggctttg atagctgaaa
    27961 caactgctta caaattgtta aaaaacgata ggacgcttaa tgagctgttg gataagctta
    28021 gaggcggtcc ttttaaaaac ggatttgatc agggtatctt tacatatgat attcctgaca
    28081 atcccgttga tttaagaaaa gtagaactag cgccatttat gcgtataaat acaacgtatg
    28141 atggtccgtc cctatatgca gatgatgaca tgatcagtaa tgaacagcgt attacaatca
    28201 atttttggtg taaaaccgct tctcagtctg accagattgc cagctgtata gataggattt
    28261 taaaaaatgg cggttttgaa agatacaccg caaacgaaaa accaaggtat aaagatagcg
    28321 atattgactt actcatgaat gtaaggaaat accgctattt tgatttttat taagaaaaga
    28381 ggaactaaat gggaaaagta aaatttggac tacgtgactt ccagtatgct gtgcttgggg
    28441 ataatgataa agttaatgaa aaagacaaag gaatcaaagc tctacccggc atgaaatcag
    28501 ctaaacttga tattacaaac gagcttgtaa ctgtcatggc tgatgatgga ccatatgttg
    28561 ttttatctgg tggtatcaca gaaacaaaat tggaaatcga agtccttgac ttgacttccg
    28621 aagcgcgtca agatttcttc ggaattaaag ctgacaaagg tattgaaaga tacaataaat
    28681 cgctcacacc taacaacgtc gcttgcatgt ttagaacaag tgatgaaaat gggaaagcta
    28741 tctgggttgg tttgttaaaa ggtaaattta acattcctgg catggatacc aaaacaaaag
    28801 aaggtgcgcc taacccagaa ccagacaaag taacaggtaa ctttgttgct cgtggaactg
    28861 atggtgatgt agttgtcatc ggtcgtgaag gagtgaatgg atttgagcta aaaaaattca
    28921 aagaacttgt gtttgatggg gtgacaggat tggaaaaatc atcagaagac aatagtctaa
    28981 tgtaacactt aaggttgggt tttaaatcca gcctttattt tttgataagg agcaataatg
    29041 tacgaaatta cacttaaaaa aggcggagta gataagactt tctcaaaaga ttttgtcaat
    29101 gtcgaagata atatgttggc ggttgagcat caagttagac agagcgctat ctttagtgat
    29161 gataagcgcc ggttagacga taaagaacat cgcaaattaa acgaatcata tttacagatg
    29221 tttgtagaaa tgtacggaaa tcaattcaat gttgacgatt tgaaacagtc taatgtgtct
    29281 gttttggaca aattaactga gctttttgtt gatgctttag gcggagagaa agaggaagac
    29341 gaaaaaaagg aacaataacg cctcaagaag ctaaagacaa cctgctcttg tggattcaaa
    29401 gtttactaaa aaatgggtat accattttag atattaaaaa aatgcgctta tcagacatag
    29461 agttgatggt aaaagcgcta gaaactgatt ttgctgaaaa agaagaagtg gttgaaacaa
    29521 ctttagacaa agcgttccca ttccttttcg gctagaaagg agaattaatg gggaatatag
    29581 gtgatttagt agcgactgct acactagaca tatcaccttt tatgtctaat acaagaaatt
    29641 taaaaactta tatgaagacc ttagataact ctctaaaggc tgtagaaaat agttttaatg
    29701 gtcatggtag tagagttaaa ggtttaaaag ctatctacac agaaactggc ggtgcattaa
    29761 aaagttatca agaattacta aaaaaacaat ctcaaaaata taatgagtta aaagaaaata
    29821 ttggcgatgt caacaaggcg acagcaagcc aaaaacaagc acttattaat gctaagtcag
    29881 cgatgatgga aacagctgct aaagtttcag aattgcaaag taggctcaaa gctttaggcg
    29941 ctgaaacaag tgtttttact cgttttggta acgctgcgga gcaagctggg aaaaaaatga
    30001 agtcatttgg cgattctgta tctggtgttg gtgctgcttt tacaaaagga gtaactgcac
    30061 caattgttgc tggcgctgga tatgctctta aggctgctgt tgattatgaa gatgcttttg
    30121 ctggcgtcaa aaagacagta gacgaagtaa aagactcaaa tggtaaagtt gtctactctt
    30181 acgatatgtt gtccaaagga attagacaaa tggctaaaga gataccagct tctacaactg
    30241 aaatttctca cgttgctgaa gcagctggtc aattaggtat caagacaaag gatgtcttag
    30301 gatttactcg tgtcatgatt gatatgggga aatcgactaa cttatcatca gaagaagcgg
    30361 caactgcact agctcgtttt gctaatatca cgcagttgga cccatcaaaa tacagtaatc
    30421 taggaagttc tatcgttgag ttaggaaata actttgcgac aactgaaaaa gaaattgttg
    30481 aaatgggtct tcgcttagct ggcactggta aagttgtagg attgacagac cctcaaatat
    30541 taggtcttgc aactgctatg agttcggttg gtatcgaggc agaggctggt ggttcggcgt
    30601 ttagtcgtgt catgcaaaaa ataaacacac aggttctttc tggtggagaa gatttatgga
    30661 agtttgcgaa aatagccggc aagtctgctg atgaattcgc tgcgtcatgg aagaaaaatc
    30721 cacaagaagc gattattgat tttgtaaaag gtcttaaacg ctttaaagaa gaagggaaag
    30781 acgtaactgc tcacttgcaa gacatggata tcaattctgt acgtgaaatt gatacattac
    30841 aacgtttggc aggagctggc gacttgctag gtgatgcttt caagtctgct aataaaggtt
    30901 ttagcgaaaa caaagcttta atgaacgaag cgaacaaacg ttatgaaacg acagcatcga
    30961 aattacaaat gctaaaaaat caagttaatg atgtcgctat tgaatttggt ggacctctta
    31021 ttgatgcact gcgtgacggt ttagaagctg gtaaacctat tattaaaggc gttgcagact
    31081 tagcaaaagc atttagttct ctggataaag agcaacaaca acaaatcata aaatggggac
    31141 taattgcagc agcaactgga cccgcattgt caatcttcgg caaaggcgtt ggcgtaattg
    31201 gtggaacgat tcaaggacta ggaaaattaa gcaaaacgtt aggcacactc tccgggagtc
    31261 tgggagcagc aaaaactgga gctgctgctt taggagttgg agcggaaggt gcaactactg
    31321 cattaactgg aatgagtgga gcggcagcat tgctagggaa tcccgtaact tggggtgttg
    31381 tgctaggagg agctgctgcg cttgctgtcg gatactttgc tcaaaaagcc tacgaagcgc
    31441 accaacgtac gcaagagtgg gggactaaag ttagtcaagt acaagccaat gaactgcaat
    31501 cctttaaaga taaggttgat caaacaaatc aatcaatgga aggctttaga gggggagctg
    31561 aacaggttaa ctccgtgaag acagcgttcc aaggtttagt tgctgaaatt gaaaagctag
    31621 aaaataaaga tttaagcgaa aaaatcaaac ttgcagaaca atttggtttt agccaaggta
    31681 cgatggacca aataaaaaaa tctagccgac aaactgtaga gaatgttaaa cagatgtctg
    31741 atgaagttat caatatctat caaaatgcta gtaatgaaca cagaagatta accgaagaag
    31801 aaaaagctgt tgtattagcg aatcaaaacg agcttatcaa tgtgcaactg tcaaaattga
    31861 actactctgc taaagagaaa aaagctatcg taaaagcaat gaatggcgat ttaaattcat
    31921 tgaatagcca gcaattaacc aaagctcttg aagttactga aaaatggata aaagcagaaa
    31981 ataagtcata caataaactc aaaagtggtc ttaaaaaagt ttacgactct ataaaaggta
    32041 atgacaaaga ggctgtcaaa actcgcgaag agatacacaa gaagcaacaa caacttgaag
    32101 cagaccatta cttaaaaatg gaagcttacg gcaagcgcta cgctgcaatt caaaagaaat
    32161 tgctaaaagg tactgctaaa tatttagacc ctcaattgca acaagcgatg gttaatgatg
    32221 ttaaaaagca aatgaaggag cttggtttgt cttacgaaga gttgatgaca aagacaacca
    32281 aagcggcttc aaaagcgcaa gaagtgaata caatgtgggc tagaaccact aagaaatcta
    32341 cagaagacca aaagcttgct aattcgcagt ggaacggact tgtctggaat cccaaaacag
    32401 gtaagttaaa aaccaatgct aaagaggaag tagccaaggc tcttgaagcg gaaggtggct
    32461 gggacagact taagtttatt gctaaaaacg caaacctaga aacaaacgct cggataacca
    32521 tggcagaagt acttgtagag actggcaaat gggataccct tactccagaa gagaaagagt
    32581 tagttgttga tgggcatcaa ggtattcaag ctatagtaga aagtaagcaa catttagaaa
    32641 tatggaatag tttgccagaa gaagttaagc ggattttagg ggataacaaa gacttcctga
    32701 ataaaaaagg agttgcaaca cgaactcttg aaaattggaa tgctttaaaa ccagacgaaa
    32761 aaaaattact cgctaaaaac ttaacaaaaa aagggaaaga tgaagcacaa aaaacgatca
    32821 atagcttagt cggaaaagaa gtcaaagtga cagcggctaa taaaacattg tccggagtta
    32881 acagcgctca aagaacatta aatagtgtgc aagataaaca tgtcactatt tgggcgtcaa
    32941 tcaaaaaaac tgcgagtgac ttatggagca aactaacagg atacgctgtt ggtacagact
    33001 accaccctgg tggacttgcg atggtcaacg accaaaaagg ttctttatat aaagagttag
    33061 taacattacc aaacggacaa tcttttatcc cagatggacg tgacgttatc ttgccactcc
    33121 ctcgtggttc gaaagtcatg aaagcaagtg ctactcgtga ttacatgtat gacttaggaa
    33181 taccaaaata tgcaaacggt attggttttg acaacactaa gattgctaat atcacgcaac
    33241 gaatgagcga acttcctaaa aacacagtga cttcaacaac agatgataag ttgtattgga
    33301 tgattgagga aatgattgca gttttaaaat ctactaaaga taacagcgtt attgagcaag
    33361 ctttagacat tgcagaacaa gcaatagagc gtccagtaga actttactta caggacggtc
    33421 agtgggtagc taaagttgcg gaccgcatta caaattatca aacgcaacga aatagtcgca
    33481 ataatcgaat gaaaggaatg ctaaattgaa atttatttat gacggtgtag acatgtctaa
    33541 attttttagg atatctcgtg ttgagcggtc tataggaaat gaacgaacat tgtcgttgaa
    33601 tgagacgttt caacttggta cagctattcg tggcataaag acgggcgcta aaattatcaa
    33661 agtacatata gagccactag aaataaatgg cgttttaacc gagcaattaa aacatgaact
    33721 tgctggtgtg ttaaatgttg acaagcctaa aaaaatgacg ttcggagatg aaccagataa
    33781 gtactatctc gggcttgcac aaggtgagat ttcgacagaa aaagtagcaa ggtggtatca
    33841 gagggctgtt atcacttttt acatacccga cggtgttgca cactctatca cttataaaaa
    33901 gttttcggac tatacgcaag aaggcaacaa gcttattttt aatttgcaaa atgatggtaa
    33961 tactaacgct tatccaataa tcaaaattaa acataactct gaaaacggtt atatcggtat
    34021 cgcaaatgaa acgggcgctt ttgcacttgg atcatcggag gaagaagatg ggactatcgt
    34081 tcatcgcaac gaatcccttt ttgattactc aaaagctatt gcacaagctt tagaaggtgc
    34141 gcccaacgtc gcaaaactca actatatgcc accaacatat gacacagaac ttaaacggat
    34201 gcgcattgat aatatcttag gctctggtaa aggcggtgaa tatgttgtta ttggaaatag
    34261 agggactacc ccgggctaca ccgagcacat tgggactcga acgtttatta tcaatcctga
    34321 ttcaaacgga gaatacactc tcaatgagca cctatggtgg caacagattt ttattgctac
    34381 tgcgcaggat cagaaagggt ttttaaagct ttgtgtaacg ggaatcgacg atgaaggaaa
    34441 tgacgagttt ttgtatggaa tcgaaactta caaacgaaaa aatggttttg aaacagaata
    34501 caattttttt gctcttgatg atgacggtgt aggctggaga ttttataagc agtttaaatt
    34561 ccaagcagac agaaattatc acaatccttt ttcaatgaat agaagtagag cggttgagat
    34621 tttcagggaa gaagacaagt ttcgtattta ttttaacggt gcgcatcatc atgtaactgt
    34681 tccatctctt aaaggaaaaa aatctcgcaa gatacatctt gcaatgggga catgtagtga
    34741 tagctctaaa tatatcaact acaatctttt cgaaaaggtc aactttgaaa aaatgggagt
    34801 gtctcactac aacaacattg ttaacaagta tcaaccaggc gatgaagtgg ttatcaattt
    34861 tgaaaatgac acagtcaaaa caaaagactt agattctatt caagacatgg ttttaggttc
    34921 gcagcctata tctataccac ccggaaaaac agagttcgtc atgcagctat ctaaattttc
    34981 tcaatatgcg ccaaacgttg aaatattaat ggaagagagg tggttataat aacgctagta
    35041 atacatgatt ctaagttgca ccctgttttg ttgctagaca atgaaaaaca aggtacgctt
    35101 aattattttg acgacacatg gactagacag ttaactacag gctcatctgt ttttgagttt
    35161 tctgtgtaca aaaaaacact tgaaggagac aacccactca atcataagta tcaagtgctt
    35221 aatgaccaag cgtttgtatc gtttgtacat aaagataaag tacagctctt taatatcatg
    35281 cagatcgaag agactgaaac gactgtacgt tgctattgcg aaaatcttaa tttagagtta
    35341 ctaaacgagt attgcaacgc atataaagca actaaagcaa tgtcatttga agagtatctt
    35401 gtgcagtttg atattttaaa ttggggtgct ttgacaattg gcacaaacga agttaaggac
    35461 aaaaaactga cattggaatg gactggtcaa gacactaagt tagctcgtat tttgtcaatt
    35521 gctaataatt ttgatgcaga aatcgaattt gaaactcaat tacacaacaa tcacacgttt
    35581 aaagcgttta ttgtaaatgt gtacaaggaa tacgaagagg gcgtgtccta tggcgtaggt
    35641 cgtgaccgca gcgacatagt gttgagatat caaaaaaatg taactggtat cactaaaaaa
    35701 ttagacaagc gccagattta caacgccata cgcccgtatg gcaaaaagac agtcaaaggc
    35761 gagcgcgtta tttctaatcc tgtaactcgc aaagtcacta aaacagttgg gtcaaatcgt
    35821 acatatttag gcggagacct caaatattat ggtcatacaa tcaaaaaagc taacgtacaa
    35881 tctattatta actacgcggt gcagtataat attttgccga gtggaatcat atgtcaactg
    35941 tacttagaaa gtctttgggg tgattcagca gttggtaaac gtgacaataa ctgggcaggt
    36001 ataagcggcg gagcacagac acgccctagt ggagtaaaag tcactactgg aatggctcgt
    36061 cctcccagcg agggtggaac atacatgcac tacgcaagtg ttgatgactt tttaaaagat
    36121 tatacttatc ttttagctaa acaaggacta tataacgttg ttggcaaaaa gaatatagca
    36181 gactatacaa aaggtttgtt tcgtgtcggt ggcgctaaag atgattacgc ggcagcagga
    36241 tatcaaagct acacaaattt gatgactaat atccgaaatg gtatcaataa agtaactgga
    36301 aatatcctca atacgattga taagctgtgg caaacaccag tacagcctat aacagccgta
    36361 aacgtagcta gaagagctac taagacaatg caagcactaa atgaagctac tagacttaaa
    36421 ggtcgcagaa tcggctcagg acagtgttat gctttgtctg gttggtacgc taagaagtta
    36481 gacggagctt ggattgatag ttccatcggt ggtatccgtg gtcgcattgg cggtgggatg
    36541 gctgctgctt taatcggtac tgactataac tggggggcgt atggttggaa gctagacagg
    36601 tcgcctaatg ctggcaactt gcaagctggc ggtatctata atgttaaagc aaattttggt
    36661 gctccgtttt atacaacaca atgggggcac acagggatta tcaagagtgt gtccaagacc
    36721 agagttactg ttttggagca aaactttgtt ggccgcatgt atgttgtcga aaactcatat
    36781 gacattaact ctttcgcatc tggattgcag acagtatgct atcctcgtga gattgcacaa
    36841 ggaatggctg tcaacggtgc aacaacacag caagttagtg gtgggacaca gatatcatat
    36901 gaggaagtcg tacaagaagc acagacagaa tcatacgaag aagaacaaat catctacatt
    36961 gacaactcaa tctacaaaga gtggaaagat gaaaacggta aagtagagta ctatctcaaa
    37021 aatggatttt tgtacgcacc tttatccaga gaccgttatc catctgtttt aacaggtaat
    37081 gagacacggg ataactggat acgaaaggac atggaaattg agactgatag tcaagacgtc
    37141 ttgatgtcaa caggtctaaa agacttaaaa gcacacgcct atccagcaat cacttatgaa
    37201 gttgatggct atgttgattt agaaattggc gatgtcgtac gcattcaaga tgatggttat
    37261 caaccacctt tgattttgac tgctcgtgtt attgagcagg ttttgtctaa agataatcca
    37321 aactcaaaca aaactaaatt tagtaatttt gttgagaaag aaagtcaact agcgtctgat
    37381 ttaatcagcg atatgttgcg tctatacgat gagtcaattc cgtatgaaat taaactagcg
    37441 acttcgaacg gtgtcgcttt taaaaatggc actggtgaat ctgtcttaac tccaagtttg
    37501 caaaaaaacg gaaaagacta tgaagctgtt tatttttaca aaaatggcga ctcattaatt
    37561 gatatcggac catcactaat tgttaaagca agcgacttta accacgtttt aaacgtaaca
    37621 gtagaggcgt atttaaatga ggagcttgtg gcaagtacac aaatatcatt tacagacaca
    37681 gaagacggtg cagacgggaa tacgccttac tggcatgttg cttatgcaaa tagcgcagat
    37741 gggacagttg attttagcgt gtctgatagc gcaaacaagc gctacattgg gcaatatact
    37801 gactacgatg caataggctc aaacgaccct aaaaaatata agtggactga tatggttggg
    37861 acggtcgtcg ttgggacaaa taatctaatc gatggcacta agtcgtttag cggagaagac
    37921 tggtttactt ctgcaacact agaagatgaa aatatctcta attatccatt tacatttaaa
    37981 aaatggataa gcggtcaaaa ggtatcacac gcaaaagaca ttatggttga gcaaggtgtg
    38041 acatacactt ttagtgctta tgttaaacgt gaggtagctg ggaatttata tttttatctt
    38101 tatgatatag cagatggttt tattactagc gataccccac gagagacaat tataaaaaac
    38161 gttgactcta gtctcagacg ttttgaaatc acttttacac caactaagac aggtaggatt
    38221 agaccaaggt tcgcgatggt gtcatcggag caaggtagtt tcagctctgg tgggtttatg
    38281 ctcgttaggg gaaataaaac aggcgactgg caggaatcag aagctgacaa agctagtaat
    38341 ttagatgcaa aagccgatgg tgcttttaca gttgagcaac taaatgcact cgcagaacgt
    38401 gctcgcattg cagaagctga attgcaatct aaagcgacac tagacacagt aaacgactgg
    38461 gttaaagcac tacaagacga aatcaaagca cgagaagaag gacaaaagct atcagaacaa
    38521 aaactgatag actcttctaa tcgcatgata gcagtacagc aaacgattgg tgaaatgcag
    38581 gttcgcaccg attttgtcaa taaatttatg agtcagtcgg aagatgggct tgttattggt
    38641 caaaaagacg gtacttctag tgttcgtgtt gataacgacc gtataagctt ttactcaagt
    38701 ggtaaagaag tcgcttatat tgctcaaagt gtgttagtta ttgatagtgg tatctttacg
    38761 accaaactac aaatagggcg ctatcgtatt gagcaatacg agctaaatcc agatataaat
    38821 gttgtccgct atgttggata ggaaggaggt taaatgacaa cttattatag taactctgat
    38881 aggagttacc gcttaactta tattgttgac gaggtctcaa catcaatcgc agataatagc
    38941 agtcaagtta gatttagact ttatttaaca tcaaggacta acagttacgc acaatacaac
    39001 tttagcggat atgcttgggt tggtggtaga tatgacttta gcgccccatc tgcgcttggc
    39061 tttaacagta attatttact gattgataaa actatcaaag taccacacga tgctgacggt
    39121 agtaagactg tggttgtcgc agctaagttg tcaggtcctg gcgggtatgc acccggaacg
    39181 ctaaatatac cagaccaaaa atttacgctg actaaaatcc cacgaacaag cacagttgct
    39241 gtaagtagtg gttattttgg tgatacgcta aatgtcaaca tcaataaaac aaatgacagc
    39301 tttacatacg atgtcagata taacgttaac gggataactg gtactgtggc tagtgatata
    39361 tcaggatcaa cgacttttaa aactagtttg gattgggcta gtacgattcc aaatacaact
    39421 agtacaccag ctacaattta cgttgacact aaatcgaatg ggtcggtcat tgggacatca
    39481 acaggtattt tttatctaac tgtacctgat aacatcaagc ctaaaatttc cagcctcact
    39541 ttatcagaca cgaatcaaaa agcatctaca attgtaggtg ctaataattt tgtccagata
    39601 atttctaatc cagtcgtcac atttaatggg gctagtggtg cttatggctc tacaatccaa
    39661 aattttaacg ctgaaattat aggtaaaaac caatctacgc agtcaaacgg tgggtcatta
    39721 ggcattttaa actttagtgg taaagccaca gttaaagcaa cagtaacgga cagtcgcggg
    39781 agggtgtcag accctgtgac aacagaaatc aacgttatac catattcccc accagctttt
    39841 agttttactg taacacgcgc aggcgctaaa aatgaccaac tagttgtcac tcgtaacgct
    39901 aaaatctccc cgctgattgt cgatggcgtc caaaaaaata aaatgacgct gacttttaaa
    39961 acagcaccac ttaataccac gagttttaca atagacacat caaacgcaag cggtacttat
    40021 actacagtcg cagagttgat taactcaaca gctacactta gtggttcgta tggagctaac
    40081 aaatcatttg atgtctacgg tttgcttagt gatgttttta gcgcaagtgg aggcggtaca
    40141 cctgttaaac aaacggtatc aacagagtct ttcccgctgt catggcataa aaacagcgtc
    40201 ggaattggaa cattaccaaa aattgatgat acaggttctt taaatgtcgc aggcaatatc
    40261 tattctgatg gcaagcaaat ccaacaaaaa caacttgctt taaataatgg tggggcattt
    40321 aggcatgata caacagactt aaacagcttg caagacacag gtttttattg tgtttttaaa
    40381 ggcgataaca gaccaagtgg tgctggaccg ggctatctaa cagttgtaag acacgagaca
    40441 gccaattacg cttaccagca tttttatgac cgcacgaaca aaaccatttt tacacgagtg
    40501 ctagaaaacg gggcatggag tggttggagt gagtacgcta aaaaagatag cttaccgcaa
    40561 tccgcaccag cggtagaaga tactggttgg caatacatcg gcaacggttt taattacagg
    40621 aaaattggta gcatggtcac tattaaatat gactttgcaa caaatggaat aaaccagttt
    40681 acggtcggtt ccatgccaac gaatttaatt ccaaacgaaa tgatgtttgc ggttactgcg
    40741 tggactgtgc aattaaatgt attaaatgta caagttagtg cagatggtcg tattttatgg
    40801 ttcaacccat caaaatgggc ggttaatgtt aaaggacaaa ttaattggat aatttaaaag
    40861 gaggaattat gcttgaattt ttgaatagat acccagtttt actggaagat aaaagtgtaa
    40921 aagagactaa agcgatttta gcatttacgt ctagcacgat taaagcaaat tttgaagtga
    40981 cgctaccagc agaagaaaat gataaaaaat ttgctgaaac tttaaaaacg tgtgaaaagc
    41041 ttatctttga gcaactttac aaagacaaag cagaagcaga acaatttgaa aaaattaatg
    41101 acgcaattgc taagtcaaag gcgcaatcag ataaagcgga aaatatgatt aaactgatgt
    41161 cagcaactgt taacgatttg attaagacaa tggctgacgg agggaaattg aatgatacaa
    41221 cgcttaacaa cgctagcgaa aatagcagta cacatattta aaaacaaaaa aggagaaaaa
    41281 acaatgatga ttaattactt tgcaatgcag attgaactag ggtggattac tattgatgac
    41341 gttccagcat tttgtcgtga gcgagtacgt aaactaattg aagtttctac ggttggtaca
    41401 gaaggaaaat gaggcaatga atgaacattg acatactaca aattggcgca gcaagcgggg
    41461 cgattttatc ggtagttgga ttgtgggcgt ttgttgttaa tccgtttaaa acagcgatgc
    41521 aaaaaaacga agatacaatg agcgccctta aagacacaat aaaagaactg gcttacgaac
    41581 taaaagactc acagcgtgac agggaaaaga tacataaaat cttggatatc cacgagcaac
    41641 gactcggaaa aacagaagac gacatcattg tcaacaagga acaaataaaa acattattta
    41701 ataggagaaa taaatatgat taatttaaaa ttacgactac aaaacaaagt aactttgatg
    41761 gctattttag gagctatatt tttgctagca cagcaagtag gtattaaact accatcaaat
    41821 attgcggata ttgccaacac agcagtaacg cttttggttt tgcttggagt tgtgacagac
    41881 ccaacaacaa aagggctatc agatagcgaa caagcattga attaccacga gccaaaaaaa
    41941 taggagggga catgcgagca atcactaaaa tagcaatggt actagcgata gcaatactgt
    42001 acataccgct tgcagtgatt gcttttttta tttatccgat ttatttgatt tttgaaaagg
    42061 aggagtaaat ggcaacgtat caagaatata aaaacaggtc aaatggtaac gcttatgaca
    42121 ttgatgggtc gtttagtgca caatgttggg acggttatgc tgattactgt agatttttag
    42181 gcgtaccgta cgcaaactgt acaaacacag gatacgcaag agacatttgg gaacaacggc
    42241 acgaaaatgg cattttaaac tactttgacg aggtagagac tatgcaagct ggggatgttg
    42301 ctatttttat ggtcgttgac ggtgtgacac cttatagcca tgtcgctatt tttgatagtg
    42361 atgctggtgg tggctacgga tggttttttg gtcaaaatca aggtggaaca aatggagctt
    42421 ataatctagt aaaaatacca tattcagcaa cttatcctac tgcgtttaga ccaaaagttt
    42481 ttaaaaacgc tgttactgta actgataata ccggtttaaa taaaggtgat tactttatcg
    42541 atgtatcggc ttatcagcaa gcggatttaa ctgctacttg tcagcaggca ggcactacaa
    42601 aaacgattat caaggtatca gagtctattg cgtggctatc tgacagacac caacaacaag
    42661 ctaacacgag tgacccgatt ggctattatc attttgggcg ttttggcggt gatagtgctt
    42721 tagcgcaaag ggaagcagac ttgtttttgt ctaacttacc aggcaaaaaa gtatcatact
    42781 tagtcattga ctacgaagat tccgcaagcg cagacaagca agctaacaca aacgcagtta
    42841 ttgcatttat ggataaaatc gctagcgctg gttacaagcc tgtttattac agctataagc
    42901 catttacact taataatgtt gattatcagc agattattgc taaatacccc aacagtattt
    42961 gggtcgcagg ctatcccgat tatgaggtgc gctctgaacc gctgtgggac tattttccgt
    43021 caatggacgg tgtgcggtgg tggcaattta caagtgtcgg aattgctggg gggctagata
    43081 aaaatgtcgt tttattagct gacgacaata gccaagtgac tgtaccaaaa gcagataaac
    43141 cgcaagaagc agttagcttt aatcagcgtt tagatgttaa tactaagcta gataactcaa
    43201 atacgccata ctacgaagca acccttagca cagactatta tgtagagtct aagccaaacg
    43261 caagtagttc tgataaagaa tttatcaaag caggaactcg cgtaagagtc tacgaaaaag
    43321 taaatggatg gtcacgtatt aacgcttctc agtctgacca atgggtcgaa gataagtatt
    43381 tagctaatgc cacacaagta taaaacagga ggtaaaattc ctttagataa gacaaatgcc
    43441 ctcgcatacg cgcagacatg ttctgatggg gggttttttt gctaaaaaat tgagggtgtt
    43501 tcatggcgga aaattttcaa cacaaaagtt acagagaaca agttgaactt ttggaatcta
    43561 gaggtattgt ctttagtgga agaaaagcta aaagcaaaag ttgaacaagc gtcaggcagt
    43621 cttaaagaag gtgcagggaa gctaaccggt gataaagagt tagaagcaaa aggatttgtc
    43681 gaaaaaacaa ttgctaaagg taaagaacta gcagatgatg ctaaagatgc tgttgaagag
    43741 gcagtagatg ctgtcaaaga aaaactgaaa taaatattaa ccgctcatta aatgagcggt
    43801 tatttttttg tctatcagaa cagaaaaatt taaaattgtc tatttttagg attttt
//
LOCUS       DATROQ010000006        43355 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.006, whole genome shotgun sequence.
ACCESSION   DATROQ010000006 DATROQ010000000
VERSION     DATROQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43355
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.006"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            58..1953
                     /locus_tag="VBC94_000277"
     CDS             58..1953
                     /locus_tag="VBC94_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HEO6978580.1"
                     /translation="MIKYQDDFYQAVNGEWAKTAVIPDDKPRTGGFSDLADDIEALML
                     STTDKWLADENKPSDTILNHFIAFHKMTADYQKREEVGVSPVLPLIEEYKGLQSFSEF
                     ASKVAEYELEGKPNEFPFGVAPDFMNAQLNVLWAEAPGIILPDTTYYSEDNEKGKELL
                     ALWRKSQEDLLPLFGLSEQEIKDILDKVLALDAKLAQYVLSREESSEYVKLYHPYNWE
                     DFTKLAPELPLDAIFQKILGQKPDKVIVPEERFWTEFASDYYSESNWELLKADLILSA
                     ANAYNAYLTDDIRIKSGVYSRALSGTPQAMDKKKAAYYLASGPYNQALGLWYAGEKFS
                     PEAKADVEHKIATMIDVYKSRLEKADWLAQSTREKAIVKLNVITPHIGYPEKLPETYT
                     KKIIDPKLSLVENATNLDKISIAYGWSKWNKPVDRSEWHMPAHMVNAYYDPQQNQIVF
                     PAAILQAPFYALEQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNNWWTDEDFK
                     AFKKLTDKVVEQFDGLESYGAKVNGKLTVSENVADLGGVACALEAAQRESDFSARDFF
                     INFATIWRMKARDEYMQMLASVDVHAPAQWRTNITVTNFEEFHKEFDVKDGDNMWRPV
                     EKRVIIW"
     gene            complement(2003..2956)
                     /locus_tag="VBC94_000278"
     CDS             complement(2003..2956)
                     /locus_tag="VBC94_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEO6978581.1"
                     /translation="MKKLTKGSHIRVLSPSSSIERLGGFEANLLAKEVLEKLGFQVSF
                     SKHYLENDILYSASIASRVEDLHEAFADPSVDAILATIGGFNSNELLPYLDYDLISKN
                     PKIICGYSDSTAFLNAIFAKAKIQTYMGPAYSSFKMKEGQPYQTQAWLTAMTENHYEL
                     WPSEEWSSDPWYDPSKPRQFFPTEWKIYNHGKASGTIIGGNLSTFGLLRGTPYAPKIE
                     RYVLLIEEAEESNFYEFDRNLAAILQAYPHPQAILMGRFPKECGMTPQVFEYILSKHA
                     IFKEIPVIYDMDFAHTQPLLTVTIGAEISVDTTTLSLSIKE"
     gene            3123..3359
                     /locus_tag="VBC94_000279"
     CDS             3123..3359
                     /locus_tag="VBC94_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978582.1"
                     /translation="MSQLSLEERLNRLKDKYLSSKNQEFSNVHLSKVKKKKKKLIILE
                     KERCRCRLEREDTKKIDDKIEKLKKELIDVMKEE"
     gene            3361..5430
                     /locus_tag="VBC94_000280"
     CDS             3361..5430
                     /locus_tag="VBC94_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEO6978583.1"
                     /translation="MEETILIVSFLLFLILSNVINRIFPKLPLPFIQLVFGILSGLVF
                     HKSQVHIDPELFLAFVIAPLNFREGQESDIGSFVKYRTIILYLILPTVFLTAIVVGYV
                     AGHLLPVSLPLAACFALGAALGPTDAVAFISIAKRFQFPKRAENILKLEGLLNDASGL
                     VSFQFALTALVTGYFSLAKASLKLALAIMGGFLIGLLFAFLMRLCLTVLEKFDAADVT
                     GALLLELTLPFVAYFVADLLGFSAIIAVVVAGVMQANRLKKVTLFDAQVDRVTSVIWE
                     TLNFILNGLVFLIFGRELIRIIGPLLTSNAYSNFDLISIVVLVTCTLFLVRFLAVSRF
                     YAWRSFKYHKSFKKYWREIQLLTFSGVKGSVSIATILLLPKHSVIGELGYSLILFTVG
                     AVTLMSFLTGLLVLPKLAPPLQVKDDYLIRLSILTKVLSVLEEDGKSSENQASFYAVI
                     DNYNSRIRHLILEQESSDIKKDLAELQLMMLSIESDGLEAAYRYGNISIKEYRIYQRY
                     LKYLERQVNRSFVSNITYSLTILMRVIRGIVHEFLTFGQGIRNRKKHFKNRKRLLAEV
                     NRQHLTDLYLSNNDLILETLEDMEGYYNATLIDFLKNERIRDAELIKSGLFVERVIAR
                     ITPDILNETLRGYYLERRTISEFERDGLISSKESQILRDNVNELESYSLRDSQSSIPY
                     QLLDLKK"
     gene            complement(5574..6641)
                     /locus_tag="VBC94_000281"
     CDS             complement(5574..6641)
                     /locus_tag="VBC94_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6978584.1"
                     /translation="MAYYRKRDNGWEYRISYKDESGKFRQKSKSGFKTKKLAQAAARD
                     IEKKLSQNILTDGEVTLYDFVKTWSEVYKRPYVKDKTWETYTKNFRHIKTYFKDIKVK
                     DITPLYYQKRLNEFGEKYAQETLEKFHYQIKGAMKVAVREQVIHFNFADDAKVKSQIE
                     SRAEENDFLEESEYKALLSLTRENIQYVSYFTLYLLSVTGLRFSEVMGLTWSDIDFKN
                     GILDINKSFDYSNTQDFADLKNESSKRKVPIDSNTIDILREYKKNHWQANIKNRVCFG
                     VSNSACNKLIKKIVGRKVRNHSLRHTYASFLILNGVDIVTISKLLGHESPDITLKVYT
                     HQMEALAERNFEKIKNIFLVA"
     gene            complement(6765..7169)
                     /locus_tag="VBC94_000282"
     CDS             complement(6765..7169)
                     /locus_tag="VBC94_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HEO6978585.1"
                     /translation="MALFGGKDKEIEVELFTSEPNERVFEFKKSKTIVRIDDYFIRIA
                     RKSNMSNMLLHGLDGEKSILLSEITAYQLKEPSSTVGYLQLVYPGSSDTKGGVFDAVK
                     DENTVTFSKDEKIAILELKNAIENALKNKVKK"
     gene            complement(7191..7937)
                     /locus_tag="VBC94_000283"
     CDS             complement(7191..7937)
                     /locus_tag="VBC94_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO6978586.1"
                     /translation="MKYTPLSREQYFDYHNKAYQIISSIDKMKENVSYQDVIKHFEQN
                     YPILFNFLDYDEMKERYPELPDYHPTDKDIKYRGLVSNRTVTYTDRVLCESCAGLTVP
                     DLDLGRYIIYINQHTNTKGRVIFTILHELSHIHCHLNQTENQPIYMSLMSKNASEKYP
                     KELIPIEKEADTVASILYLTDERLKKALTTRESFEDIQRETDISKPALHNRLMNYLIY
                     NLQYAESYALKLVLNYRQGGGQIFNILRLQ"
     gene            complement(7946..8326)
                     /locus_tag="VBC94_000284"
     CDS             complement(7946..8326)
                     /locus_tag="VBC94_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6978587.1"
                     /translation="MFFTFEKIKELADKQGISLNQLEEQLDFPRNTIYNMKKSTPNVE
                     RVSKIADYFNVSTDYLLGRTENPKIADGQDNTAVDDLTQQAIVLFRKETEGLSASEKE
                     RFNSALEGLMKTARQLIQDDSNWK"
     gene            8614..8778
                     /locus_tag="VBC94_000285"
     CDS             8614..8778
                     /locus_tag="VBC94_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978588.1"
                     /translation="MGIGSMTVTVKVTNMDKFIELSKEFNKKARELEELAHELKSFDF
                     EGEVVSTDSN"
     gene            complement(8761..9231)
                     /locus_tag="VBC94_000286"
     CDS             complement(8761..9231)
                     /locus_tag="VBC94_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978589.1"
                     /translation="MNLNQIRIIEACHKFLIGITNFEEELQDDVLVYRYKGNLVSFET
                     YQEYEQRSFVDYNLKYGYLDDTRTYIDDRADLIAAFPSEEHLRALQRVNDAEQARVQI
                     FKLLSQVNLDSLSEKNSHIKKDNFGYDFFNFATKEEYPVYLFSDDESFELVAIS"
     gene            9292..9504
                     /locus_tag="VBC94_000287"
     CDS             9292..9504
                     /locus_tag="VBC94_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6978590.1"
                     /translation="MSQQQKKWIQLVKDKLNEEQMTQTHLARACGVAKATISELLKYG
                     KGSVRLKNKVSDILHIDESWTDLEED"
     gene            9504..9845
                     /locus_tag="VBC94_000288"
     CDS             9504..9845
                     /locus_tag="VBC94_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978591.1"
                     /translation="MPITREMTITEINVLNAIKNSATYDLPIQARELRQQLGLSKRSL
                     EAVIENLRVIYKQPIVAKKKQPSGYYLPRNEEERNDGLAPYKRQILTEQNNLAAIMSV
                     NLEEYWATEKA"
     gene            9892..10422
                     /locus_tag="VBC94_000289"
     CDS             9892..10422
                     /locus_tag="VBC94_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO6978592.1"
                     /translation="MNEVWKDIPIVKGYYQVSNLGRVRSIGRTVNAKQRTRKTKGRIL
                     KQSLSSGYAIVTLSVNGLRKSIRVHRLVAEAFIPNPINKRTINHIDENKLNNRVDNLE
                     WATDKENANHGNRTTKSSLGRCKPVEQFTLEGEFINTFDSIKSASMKTGISSQRITAT
                     AMGHQKQTHGYKWRYA"
     gene            10422..10706
                     /locus_tag="VBC94_000290"
     CDS             10422..10706
                     /locus_tag="VBC94_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO6978593.1"
                     /translation="MNDLMIQMLDQFEAGLMDRTMKVMHVVTDEKYRFPLELNKSQCA
                     LMLLGTKDTGTFDERFNCHKDFPRIPNAREKYPRDEVIEWYHKNWQRTVI"
     gene            10730..10948
                     /locus_tag="VBC94_000291"
     CDS             10730..10948
                     /locus_tag="VBC94_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978594.1"
                     /translation="MNNLQIIAVGTLISVTLIESLISNIKLRKALKNKQTAGTVIAQY
                     IPQRGFIDYKTGRRVDIDPETKKEVFVD"
     gene            10957..12288
                     /gene="dnaB"
                     /locus_tag="VBC94_000292"
     CDS             10957..12288
                     /gene="dnaB"
                     /locus_tag="VBC94_000292"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO6978595.1"
                     /translation="MDELKVLPHDIQAEQSVLGSIFIKPEKMIEVAEYLKPNDFYRPA
                     HKILFKAMVSLADRGEAIDIVTIKSTLESTDELGMVGGISYIAEIVNAVPTSSHAEHY
                     AKIVAKKAQLRSIIDNLSDSIGNAYDEDMDIDEIIAKAERSLIEVSQASNKSSFRPIH
                     DVLLENHSKIEERSNNTSQITGIETGFYDFDKLITGLHEDQLIVLAARPAMGKTALAL
                     NIAQNVATKSNKAVAVFSLEMGAESLVERMLSAEGTIINHHIRTGNLTVNEWQRLIYA
                     QGQLAEAPIFIDDTAGVKITDIRARARRLSQETDGLGLIVIDYLQLIQGSRSDNRQQE
                     VSEISRQLKIIAKELKVPVIALSQLSRGVEQRNDKRPIMSDLRESGSIEQDADIVAFL
                     YRDAYYQDKKEGQPENDITELIIRKNRHGNLGTVKLYFHKEYTKFSSVEEE"
     gene            12290..12892
                     /locus_tag="VBC94_000293"
     CDS             12290..12892
                     /locus_tag="VBC94_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978596.1"
                     /translation="MKKFDHYFSNHIAVKNESDTVTIVDYYNPEKGYEYNLRYIFDRK
                     NSSLAITGDFGELVARNFYNMGEWDTFYNHYTGHLGYFLEKLLCSSRPVHYYDTDKAK
                     REILNEFFDGADYDDLDFDDEFLLDQLFDNFDEDKGFTYILPEAFERFSEDMEEYEVY
                     EFLSRAGRYVNDVFELYLDAYKRAYEYLTKEVDDGTTQNV"
     gene            12870..13616
                     /locus_tag="VBC94_000294"
     CDS             12870..13616
                     /locus_tag="VBC94_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HEO6978597.1"
                     /translation="MAQRRMFSRKITETDRFLEMPLSSQALYFHLNMGADDEGFIDKA
                     KTIQRTIGASDDDMKLLIAKGFLIPFDSGVVVIRHWRIHNYIQSDRFQSTLYQSEKAQ
                     LEYDKSKTSSLKPIGNCIQNVSKMETQVRLSKGSLDKDSLTTYPTVSDNEEEDIPYKE
                     IISYLNEKANRNYRPNIQKNKTLIKARWSEGFRLDDFKHVIDTTVKDWSGTKYEKYLR
                     PETLFGSKFEGYLNQAPRIKTETIDGRLGF"
     gene            13617..14438
                     /locus_tag="VBC94_000295"
     CDS             13617..14438
                     /locus_tag="VBC94_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6978598.1"
                     /translation="MNPFKNFETRQVLEETCEVHGCQLWLTKVPIKGRLEELKQCPEC
                     TKAAINIFENKLNSQSKINSKLADTYAVFERDSLVSDKLRGKSLENYEIKDDIDQQAI
                     NYAKRMEQFYRQDRTGNAIVTGPSGVGKSHLTYGLAKFMNEQFKAYESPKSVLFISLV
                     SLFTKIRESFKADNGYRQAEMIELLTSVDYLFLDDLGKESRKGDSQNNEWTHQILYEI
                     LDNRSNTIINTNLSSKEIKALYADNYGNGALSSRILEGVTGNSFAYPKDMEDRRY"
     gene            14438..14686
                     /locus_tag="VBC94_000296"
     CDS             14438..14686
                     /locus_tag="VBC94_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978599.1"
                     /translation="MSYKATFMLYGYKAVPIGLFDTKELAAQAIVDHIIENSGIPRPR
                     VVATMKIRSKEIRIDYGSKTCYYLITLINNKTEEKQHD"
     gene            14679..15173
                     /locus_tag="VBC94_000297"
     CDS             14679..15173
                     /locus_tag="VBC94_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEO6978600.1"
                     /translation="MTKTMTLEEKVEQWFIDRNLHEANPVKQFQKMMEETGELYSGIA
                     KDKSEIIYDSLGDMQVVLIGIEQQIKNGAQIEASPQDMELLLLASSLGEMAQKLYKHI
                     FHNETKTPLIRPELSLLHSTIHAIAIHNLTTADDCLSIAYEEIKDRKGKLINGIWVKE
                     EDLK"
     gene            15170..15499
                     /locus_tag="VBC94_000298"
     CDS             15170..15499
                     /locus_tag="VBC94_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEO6978601.1"
                     /translation="MKNMNWKAFFNNLKAFASVIIITVFVLGFNYLFYEIGYQKGHQE
                     ADRVIIYVADNAGAEMFGKITDKEIIEGRHTVTAGAYGKFLVTEEQYNEITVGDDIPD
                     YLKGRGN"
     gene            15499..15918
                     /locus_tag="VBC94_000299"
     CDS             15499..15918
                     /locus_tag="VBC94_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEO6978602.1"
                     /translation="MTKCNFEAGHKPIDTIGQLAKEVLDGKYGDGEERRRNLGCLYDL
                     VQTRVNYMVHERDSKFTGIKIYDNVNKPSHYQGKNGMEAIDVVRNFIGQLTGVSAFYW
                     GNSMKYLLRFQGKNGLEDLKKARKNLDWLIEEMEKEK"
     gene            15915..16121
                     /locus_tag="VBC94_000300"
     CDS             15915..16121
                     /locus_tag="VBC94_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978603.1"
                     /translation="MKKSIGILEFKEFDAFVGGKVMPVYLHSANPNVPTTNKNEAMRV
                     TKKRYRKEFSDKYIFRKVGEVDDQ"
     gene            16111..16527
                     /gene="ssb"
                     /locus_tag="VBC94_000301"
     CDS             16111..16527
                     /gene="ssb"
                     /locus_tag="VBC94_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6978604.1"
                     /translation="MINNVVLIGRLTRDVELRYTPSNIANATFNLAVNRNFKNAAGDR
                     EADFINCVMWRQQAENLANWTKKGMLIGITGRIQTRSYENQQGQRIYVTEVVADSFQI
                     LEKRDNSTNQASMDDQLPPSFGNSQPMDISDDDLPF"
     gene            16537..16797
                     /locus_tag="VBC94_000302"
     CDS             16537..16797
                     /locus_tag="VBC94_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978605.1"
                     /translation="MNEYKKPTYIVIQEAMAERIRFLEDELYNRAYKDIERLEVENDR
                     LKVRCLDLQLENADYVWDDMCRSAIARAKSENRRPHRRWRGR"
     gene            16794..17141
                     /locus_tag="VBC94_000303"
     CDS             16794..17141
                     /locus_tag="VBC94_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6978606.1"
                     /translation="MKLRLEELRNKLGLSRRDVHEATGISMNTLLHYEKGGTPSIGQV
                     ENIAKAYNINPAWLLGWIDEGQQPQPLETVVEKIIYVEKEGCRLPPYWNNDNNGRIIK
                     WKESRRAFQRRTI"
     gene            17138..17602
                     /locus_tag="VBC94_000304"
     CDS             17138..17602
                     /locus_tag="VBC94_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978607.1"
                     /translation="MTKKTASKTRRDFLEFELEAKYLKIDKLIGQRRHELERIYAVKN
                     LTMPGIDDSGASRSGTSTNTSENLAVAYASDPMILKLEEFQNAISELLEVLEPDDKKI
                     FHLRWGEHTGYDWIQVWHIMENGETGYLYRHSKQIYRRREVILDTLAKLLFM"
     gene            17712..18254
                     /locus_tag="VBC94_000305"
     CDS             17712..18254
                     /locus_tag="VBC94_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6978608.1"
                     /translation="MNIVEPLRDKDDIQAMKDYLSSWNEKYYMLFLLGINTGFRVGDI
                     LKLKVKDVQGWHIKVREQKTGKYKSIKMTRPLKNELREFVKDKELHEYLFQSRVGKNK
                     ALSYKTVYWFLKRAAEDLGIDNVGTHTMRKTFGYHYYKKYKNVADLMSLFNHSSPAVT
                     LIYICVRQDELDTKMSNFSL"
     gene            18544..18831
                     /locus_tag="VBC94_000306"
     CDS             18544..18831
                     /locus_tag="VBC94_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978609.1"
                     /translation="MKDENAFDGIRLEAGSTLEIYLTKNDLEHIANGYEVTLDIKPNE
                     TVNKIVIKPSFVNNILNPLINYDKRIVSEADLKFRDISREVARDSFALGSM"
     gene            18831..19190
                     /locus_tag="VBC94_000307"
     CDS             18831..19190
                     /locus_tag="VBC94_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO6978610.1"
                     /translation="MGYRNPKHSDWFRAWQIKFYNSKPWRTLRNKIRKAKRMRCDMCG
                     RLIHGKSIVDHIIEIDETNYQDESITLNEENCQLLCLECHNTKTFQSKINLNLDNRNI
                     NLFLIFLFLNFCGLPPF"
     gene            19278..19763
                     /locus_tag="VBC94_000308"
     CDS             19278..19763
                     /locus_tag="VBC94_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978611.1"
                     /translation="MILKINEVLEKLGISRATLTRYRKKLGIFEETRSNITKSQFKEL
                     EKLANQRQKYTRQERVELSRKTFKLIPKEKMLEISDNDSVVLKNLKTQYNHNQKVIEN
                     FQLEINKVINDGELPDKYLLDGMEKYQKLNMQIMSTIEKQSPQGDSLKEMIQEKLARY
                     G"
     gene            19756..21468
                     /locus_tag="VBC94_000309"
     CDS             19756..21468
                     /locus_tag="VBC94_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEO6978612.1"
                     /translation="MVEMRYFDKYAQLIYTGKIRICKLTMKSIRRVERYKEQYLFKQE
                     EADKRIEFIEEECSNTKGLAGKLRLALPQKVWLETTWGFYHTVEVTKTNPDTLEEYTD
                     YEERRLIHEVPIIVPRGTGKTTLGSAIAEVGQIIDGEWGADIQLLAYSREQAGYLFNA
                     SRAMLSNEESLLHYMREADILRSTKQGILYETTNSLMSIKTSDYESLDGTNAHYNIFD
                     EVHTYDDDFIKVVNDGSSRKRKNWITWYISTNGTKRDKLFDKYYNIWVDILDDKIIND
                     SVMPWIYQLDDVSEIHDPDMWQKAMPLLGITTEKETIARDIEMSKNDPAQQAELMAKT
                     FNLPVNNYLAYFSNEECKGWSDKFDESLFVGDDERNARCVIGIDLSDVNDICSISFMV
                     VRGEERHYLNKKFMPRHTIETLPKELRDKYTEWELSGMLHVHELDYNDQAYIFEELRQ
                     FMSDNRILPVAVGYDRYNARELIRLFNDYYGDICHDIPQTVKSLSNPLKVYKEKAKMG
                     KIIFDDPVATWNHANVRVKIDANNNIFPNKEKAKEKIDVFASQLDAFICYENFKEDLS
                     YYFD"
     gene            21477..22619
                     /locus_tag="VBC94_000310"
     CDS             21477..22619
                     /locus_tag="VBC94_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO6978613.1"
                     /translation="MNNYLKNLKEVFARIFRPNNRKSTRTYLQRSISYWRRNSIYLDN
                     IYNKISTDTAQLRFKHVKITRNPGGVDSMVWYEHSDLAEVLTVSPNPLEVPVVFWSNV
                     TRAMLRDGVAVVVPRWKNGRLVEIWLAKKTVTWTAESVELMLDDVAVELPLTDVWVFE
                     NPKLNVTAQLNQITELIDINLNALTEKLSDGNSSLRGFLKLPTKAADEHLKQQARDRV
                     DSMLDLAKNGGIAYLEQGEEFQELSKDYSTASKEELEFLKSQLYNAHGINEKLFTCDY
                     TEEQYRAYYSSVMKLYQRVYSEEINRKYFTKTARTQGNKLLVFFDMADMISFKDLVEG
                     GFKSKYAGLMNSNEFRETYLGLPGYEGGEVFETNLNAVRIGAEESE"
     gene            22669..23211
                     /locus_tag="VBC94_000311"
     CDS             22669..23211
                     /locus_tag="VBC94_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEO6978614.1"
                     /translation="MEKLKTFVVKSVEDESADFHFEAYASTYDNTDREGDVMAKGCFD
                     NTLKSKAVVPMCLNHDRNCVIGKHELSVDEKGLRTRSTFNLSDPEAKKTYDLMKMGAL
                     DSLSIGFFIKDYEPIDAKQPYGGWIFKEVEIFEISVVTVPANPQATVDNIKEFDMSAV
                     DKRIAQVNMRQEIMSKLAKI"
     gene            23222..24484
                     /locus_tag="VBC94_000312"
     CDS             23222..24484
                     /locus_tag="VBC94_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEO6978615.1"
                     /translation="MKKSLIELLEARQKATDELAEVKLKKATIEAKMKSSTIEDDDLE
                     QLKTDAESLVSQATAIKETIAGLDSDIEETEEELSKAAKIIKEKQKGNTPMDYLKTKA
                     AALDFVRILMDNEGSANSARKAWEANLVEKGVTNLTKILPEPVLIAIQDAFTNYNGIL
                     NHVSKDPRYAVRVALQTQVSQAKGHKAGKTKKDEDFTFLDFTINSATVYIKYAFEYSD
                     LKKDTTGAYFNYVMKELAQGFIRTIERAVVIGDGKSNSAEDKITEIKSIAEETEANLF
                     ETQEINVAGVFDNAVLETLVAGIDKMVPNTTPILVTSKAIARKLKLVKDAEGRYIDPQ
                     PFAPIATNGNVIAGFQVYIYDWMDGATNPIIAFADQAYKMIGDEAAADRFDDYDVTVN
                     RRHIELASVMGGRLAQYKSAVKFTNQAG"
     gene            24505..24840
                     /locus_tag="VBC94_000313"
     CDS             24505..24840
                     /locus_tag="VBC94_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978616.1"
                     /translation="MTILKTIKEMVEVDVEEDIFDVQLLRYINSGISYLQRNAIPVIR
                     IDKETELEGWTDIKEEDRETVLDWLHLRCVQRFDKSLMTGSAATMEWIDGEMTNLLYQ
                     LKAIYEVKT"
     gene            24837..25142
                     /locus_tag="VBC94_000314"
     CDS             24837..25142
                     /locus_tag="VBC94_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HEO6978617.1"
                     /translation="MKSSRVAIILCYDERVEVEKGVWEKKTVEKKVKAKKEKIYQRRL
                     DKAMADGQVLTARFSVRSNYVTDTLDYVKYNGKEYKVNIGTESDDGHYTIIELGELK"
     gene            25142..25489
                     /locus_tag="VBC94_000315"
     CDS             25142..25489
                     /locus_tag="VBC94_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978618.1"
                     /translation="MAKKFFTRQDIQSILETNPLKAKVFYMEREEKSSPDNVILYYRL
                     TPGSSITADDRVHMRKVTLQVSHYHKKKLDSIENLMLSNFMCEPNQLNLKQPDTDYLL
                     TTYRLEVFTNGRW"
     gene            25476..25820
                     /locus_tag="VBC94_000316"
     CDS             25476..25820
                     /locus_tag="VBC94_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO6978619.1"
                     /translation="MGGGSVKMTPLKVDIKNQVLENIKKAAQSTENDIRTKSPRRNGV
                     YEKGWTHDIIDDAAVVHNNGKEKSLSHLLENGHATKNGGFVAPREHIRPAYIKNKEKF
                     LNDMKSIKITPK"
     gene            25835..26518
                     /locus_tag="VBC94_000317"
     CDS             25835..26518
                     /locus_tag="VBC94_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6978620.1"
                     /translation="MTYQYDTREVTHGNANGFYAKIAKTDAGALDLQKPYPFTGLRST
                     SFETSQESNAYYADNVEHVRLQGKKSTEGSITTYQIPKQFMIDHLGKKLTNSTPPALI
                     DTGVNTNFIWGYAETVTDEFGAEIEEFHIWTNVKASAPKGSTSTDETSATPKEIEIPC
                     TASPNNFIVDSEKKPVSEIVWRDDSKGTVRGKFDKLFADKSPGKLIDFINEALGTTTT
                     PTTTVSGGS"
     gene            26518..26994
                     /locus_tag="VBC94_000318"
     CDS             26518..26994
                     /locus_tag="VBC94_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978621.1"
                     /translation="MIKKELSFTAFDSYGEEREHTETVRFLYSLPAIKMYEQRTGRNF
                     FDDNQKALTAYTQLALATGVNGNLSDLTDEEKIKMMPLLMEPDFMNFLTEVIPCLYGE
                     VENGRLVQNELTAETASLAPWFGDLIDIGFFSDLFYEFNRSRAKVPQDKKKPFQKL"
     gene            27180..29915
                     /locus_tag="VBC94_000319"
     CDS             27180..29915
                     /locus_tag="VBC94_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEO6978622.1"
                     /translation="MTETFEGLYVKFGANTVEFDRSVKGINTALSSLKKDFNNINRQL
                     KMDPDNVDLLNRKLVNLQEQARVGAMKIAELKKQQKALGESEVGSAQWNKLQLEIAKV
                     ESQMKIVDKAMESTKKHIEDVGDPKSILNLNKELDNVAKELDIVNQKLELDTDNVELA
                     EQKMKLLGKQSELVGDKVQELKKKQAALGDEKIGTEEWRQLQNEIGQAEVEVLKIDRA
                     MDILGESSRSATGDIKEATSYLRADVMMDVADKAGQIGQKMVDAGKMTVDAWSEIDEA
                     LDTVTTKTGLTGDALAELQEIAKDIATGMPTSFQNAGDAVGELNTQFGLTGEKLKSAS
                     ELLIKYAEINETDISSSAISAKQAIEAYGLTAEDLGMVLDNVTKAAQDTGQSVDTIVQ
                     KAIDGAPQIKGLGLSFEEGAALIGKFEKSGVDSSAALSSLSKAAVIYAKDGKTLTDGL
                     NETVSAIQNSTSETEALSIASEIFGSKAAPRMVDAIQRGAFSFDDLAEAAKSSSGTVS
                     TTFDETLDPIDKLTQYSNQAKEGMAELGGKLLETVIPALEPLMGMLESSVNWFTSLNE
                     TDQQTIVILGLVTTAVMMLLGAIAPLVIAIGAIGAPVGIVVAAIVGAIAVITLIIQAI
                     MNWGAITEWLQSTWDSCAAWLSELWTNIVTTATTAWSNFTAWLSGLWSSVVSTGQSLW
                     SSFTSSLSNIFSSLITGAQSLWSSFTSTLSNLWSGLVSTGSNLFNNLSSTISGIFNGI
                     LSTASNIWNSIKSTISNAIDGAKNAVSNGVNAIKNLFNFQIKWPHIPLPHFRVSGSAN
                     PLDWLKGGLPSIGIDWYAKGGIMTKPTLFGMNGNRAMVGGEAGAEAILPLNKSTLGAI
                     GQSIANTMNTSNNINVNFSGVTIREEADLNRLANVVGNRIAEELQRKTNLRGGMA"
     gene            29912..30634
                     /locus_tag="VBC94_000320"
     CDS             29912..30634
                     /locus_tag="VBC94_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6978623.1"
                     /translation="MTKINELTIDGVKTSSFKCDVLVETRPNVIVSSSKTALLEHDGI
                     SGAVVQSNRHRGLIEKPYHITLIEPSDEEIYRFSALLNREKFWLENEQEPTIRLWCYK
                     VDSFEIGKDEFGAWVVDVTFICHPTKFFKTTDTQTLTGNGVLRVQGSALAFPKITVVG
                     QSASETSFTVGNQVIKLEKLSESLVMTNDPDNPSFKTASGKLIKWAGDFITVDTAKGQ
                     NVGVVLGAGITSLKFETVWGWA"
     gene            30635..34309
                     /locus_tag="VBC94_000321"
     CDS             30635..34309
                     /locus_tag="VBC94_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001918212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6978624.1"
                     /translation="MLFLLDKDVRTVKWNGIPLHEASSAIVKEETNSDFYLTVRYPIT
                     DSGIYQLIKEDMLIKAPTPVLGAQLFRIKKPIENDDSLDITAYHVSDDIMKRSITPVS
                     VVGQGCAMALSQMVQNAKTGLGDFSFTSDIMDSRTFNTTETKTLYSVLMDGKHSIVGT
                     WEGELVRDNFALSIKRSRGADRGVVITTHKNLKSYQRTKNSQGVVTRIHARSTFKPDG
                     AEDEVTLRVSVDSPLINSYPYINEKEYENNNAETVEDLRKWAEAKFTNEGIDKVSDAI
                     EIEAYELDGQVVNLGDTVNLKSRKHSADLYKKAIAYEFNALTEEYISITFDDKPGVGG
                     SGVSSGLSNVADAILVASATAQDVAVQRAVKNANAAFDAEFGKTKTKINDDIEIAKAK
                     VESFKSELSNRMDNQLLPLATEAKNLASQAQADLTRKEIELRAELNRQVTSTEAVKIS
                     LTNLSHNMDIIKQKALNDLRDAETRLKEADSVQQLATKRVEDKLTGLSTKLESFSVGG
                     YNYVIDGGEPKELMANFYGKTYDINPQLLERTSQATLSFSYEAESTSRLEVRLYKKMH
                     TGDTSKITIIVMPNFDLSPGKGFISQSFDLGGVMPDPRNQAWLVMRGTNANPLTLSKV
                     KLERGTVATDWNNRDETLKASFAEYKQTVDENLANLRTSTETLAGQLTSAESSIRQTS
                     ESFSNRLVSLETYKDSESNRASRYFEASKSETAKQLSALRTEVNGSYVDKSTYEENAR
                     GLTRRFESLSSGTQNYALGTERDVYLNVSGTTVYDLYSFGTTLAIKGIEMGDTVTIAF
                     NYFADSRTAFGSYELELYGHAGKIERVGEVQRTISNRGTYTASFVANNANFRANSLKI
                     RFTDSRLKFRVTTLRVTKGTIPADWSPSPDDLKAYSDTKLEQTANEIKASVTSLDHKT
                     LKQTDITMTSEGIVLRAGKTSNDVARAIGSYFKVTPDAIALFSSLIKVSGNMLVDGSV
                     TSRNLVTGAVETGHVKAGAITGVLLAAEAVTAEKLKVDQAFFNKLMANDAYLKQLFAK
                     SAFITQVQSVTISASQISGGVIKALNNAMEIQMNSGQILYYTDQAALKRVLSGYPTQF
                     VKFATGTVSGKGNAGVTVIGSNRYGTESTNDGGFVGVRAWNGSNIDSLDLVGDKIRLA
                     SSAFDNSDGWDVRTLDSGLKITPHNRAAERNSRIEVGDVWILKGNGSYSSLRDILNSF
                     NENFSKGPNADSYTYYHSGF"
     gene            34326..34556
                     /locus_tag="VBC94_000322"
     CDS             34326..34556
                     /locus_tag="VBC94_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978625.1"
                     /translation="MNQEQQLNQALRLTVNELTAQLANESTTKNLLAIQLTEVDQEKQ
                     QLTQQNAELQARVSELEGLLDEQTQPEIIEGE"
     gene            34559..34897
                     /locus_tag="VBC94_000323"
     CDS             34559..34897
                     /locus_tag="VBC94_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978626.1"
                     /translation="MTTTENNLLDLETITEPFDLATALKYMKENGELIRCKNAVNDFY
                     MYRDMQKRPVIVNGRRQFKDVETVWAFNQWGGTTPTINMADLFNLEYYIMTFDENGNP
                     DWTEPHLEDK"
     gene            34932..35342
                     /locus_tag="VBC94_000324"
     CDS             34932..35342
                     /locus_tag="VBC94_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978627.1"
                     /translation="MPPWLIDSTVVVAMVTVLGGLFSTIITTSANRKDQLIKHQYEDI
                     KEDLSGLIDKVKTIDHTTTETKKISEITKDGTLKIQRYRLFHDLTKEISQGYTTIEHF
                     RELSILFESYQLLGGNGEIEALFEKFKQLPIEED"
     gene            35345..35674
                     /locus_tag="VBC94_000325"
     CDS             35345..35674
                     /locus_tag="VBC94_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO6978628.1"
                     /translation="MTQITDIIISSAMGILTILAGIAVQAVKVYLIKKGGEKAVLITE
                     ILAKNAVNAVEQVATETGFKGADKLTSAKAQILAELQKYNIHMSDDDLTLFVESSVKQ
                     MHDAWKE"
     gene            35678..37084
                     /locus_tag="VBC94_000326"
     CDS             35678..37084
                     /locus_tag="VBC94_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEO6978629.1"
                     /translation="MEINTETAIAWMSARQGKVSYSMDYRDGPNSYDCSSSVYYALRS
                     AGASSAGWAVNTEYMHDWLIKNGYELIAENVDWNAVRGDIAIWGMRGHSSGAGGHVVM
                     FIDPENIIHCNWANNGITVNNYNQTAAASGWMYCYVYRLKSGASTQGKSLDTLVKETL
                     AGNYGNGEARKAVLGNQYEAVMAVINGKTTTNQKTVDQLAQEVIAGKHGNGEARKKSL
                     GSQYDAVQKRVTELLKKQPSEPSKAQEVNKPTETKTSQTELTGQATATKEEGDLSFNG
                     TILKKAVLDKILGNCKKHDILPSYALTILHYEGLWGTSAVGKADNNWGGMTWTGQGNR
                     PSGVTVTQGSARPSNEGGHYMHYASVDDFLTDWFYLLRAGGSYKVSGAKTFSEAIKGM
                     FKVGGAVYDYAASGFDSYIVGASSRLKAIEAENGSLDKFDKTTDIGDGSKDKIDITIE
                     GIEVTINGITYELTKKPV"
     gene            37291..37476
                     /locus_tag="VBC94_000327"
     CDS             37291..37476
                     /locus_tag="VBC94_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEO6978630.1"
                     /translation="MTERELKKIAKKQGFSKTNFGKGSHEVWKHPDGRTVTIPKPKEK
                     DYRPGTLNNILKVLYGE"
     gene            37537..37941
                     /locus_tag="VBC94_000328"
     CDS             37537..37941
                     /locus_tag="VBC94_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEO6978631.1"
                     /translation="MKYTYLALFEVDKENGGYNISFPDFPGAFSEADSLNEAIFNARE
                     VLEIYTIMFEDEGKEFPKASSFKALASNLASDEDVIQAISVDTELVRERERSKIVNKT
                     VTLPSWLVEVGKENKVNFSQLLQKAIREELQV"
     gene            complement(38155..38225)
                     /locus_tag="VBC94_000329"
     tRNA            complement(38155..38225)
                     /locus_tag="VBC94_000329"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38191..38193),aa:Cys,seq:gca)
     gene            complement(38359..39891)
                     /gene="ahpF"
                     /locus_tag="VBC94_000330"
     CDS             complement(38359..39891)
                     /gene="ahpF"
                     /locus_tag="VBC94_000330"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEO6978632.1"
                     /translation="MVLDKEIKAQLAQYLDLLESDIVLQTDLGDNDNSQKVKDFLDEI
                     VAMSDRISLESTHLKRQPSFGIAKKGQESRVIFSGLPMGHEFTSFILALLQVSGRAPK
                     VDEDIIKRIKGIEKTINLETYVSLTCHNCPDVVQAFNIMAVLNPNITHTMIEGGMYQD
                     EVKSKGIMSVPTVYKDQEEFTSGRATIEQLLEQLDGPLDAEAFADKGVYDVLVIGGGP
                     AGNSAAIYAARKGLKTGILAETFGGQVIETVGIENMIGTLYTEGPKLMAQIEEHTKSY
                     DIDIIKSQLATGIEKKELVEVTLANGAILQAKTAILALGAKWRNINVPGEEEFRNKGV
                     TYCPHCDGPLFEGKNVAVIGGGNSGMEAALDLAGVTKHVTVLEFLPELKADQVLQERA
                     AKTDNLTILKNVATKDIVGEDHVTGLNYTDRDTNEEKHIDLEGVFVQIGLVPSTSWLK
                     DSGIELNERQEIVVDKFGSTNIPGIFAAGDCTDAAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(39909..40469)
                     /gene="ahpC"
                     /locus_tag="VBC94_000331"
     CDS             complement(39909..40469)
                     /gene="ahpC"
                     /locus_tag="VBC94_000331"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEO6978633.1"
                     /translation="MSLVGKEIIEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLDVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQGFDVLGQDGLAQRGTFIIDPDGVIQMMEINADGIGRDASTLIDKVRAAQYIRQH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            40746..41516
                     /gene="rpsB"
                     /locus_tag="VBC94_000332"
     CDS             40746..41516
                     /gene="rpsB"
                     /locus_tag="VBC94_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEO6978634.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVAEEAKRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFELLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDVIIPANDDAIRAVKLITSKLA
                     DAVIEGRQGEDADVDFAQEAQADSIEEIVEVVEGSNND"
     gene            41610..42650
                     /locus_tag="VBC94_000333"
     CDS             41610..42650
                     /locus_tag="VBC94_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEO6978635.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDLDKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVDGNVAAVIEVNAETDFVAKNDQFVTLVNETAKVIAE
                     GRPSNNEEALALTMPSGETLEQAFVTATATIGEKISFRRFALVEKTDEQHFGAYQHNG
                     GRIGVITVVEGGDDALAKQVSMHVAAMKPTVLSYTELDAQFVHDELAQLNHKIEQDNE
                     SRAMVNKPALPFLKYGSKAQLTDEVIAQAEEDIKAELAAEGKPEKIWDKIVPGKMDRF
                     MLDNTKVDQEYTLLAQVYIMDDSKTVEAYLESVNAKAVAFVRFEVGEGIEKASNDFEA
                     EVAATMAAALEK"
     gene            complement(42809..43270)
                     /locus_tag="VBC94_000334"
     CDS             complement(42809..43270)
                     /locus_tag="VBC94_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HEO6978636.1"
                     /translation="MNYIITFLISMIPLVELRGAVPFAIANGIPLWEALAIGVVGNML
                     PVPIIFFFARKVLEWGADKPYTGKFFTWCLKKGHSGGQKLEKVAGEKGLFIALLLFVG
                     IPLPGTGAWTGTLAASLLDWEFKHSVIAVMLGVILAGCIMGTLSIIGFNLF"
BASE COUNT        13929 a         7818 c         9198 g        12410 t
ORIGIN      
        1 ctctgaattt tctgttagac tgtaagagat tacatatgag atattaagga gaaaataatg
       61 ataaaatatc aagatgattt ttaccaagcg gttaatgggg aatgggctaa gacagctgtt
      121 attcctgatg acaaaccaag gacaggtggt ttttctgatc ttgctgatga cattgaagca
      181 ttaatgctgt caacgacaga taagtggctg gctgatgaaa ataaaccaag tgatacgatt
      241 ttaaatcatt ttattgcttt tcataaaatg acagctgatt atcaaaaacg tgaagaggta
      301 ggagtatcac cagtattacc attaattgaa gaatataaag gtctacaatc tttctctgag
      361 tttgcatcaa aagttgcaga atatgaacta gaaggtaaac caaatgaatt tccatttggt
      421 gtggctcctg actttatgaa tgctcaattg aatgtacttt gggctgaagc gcctgggatt
      481 attttaccgg atacaactta ttatagtgaa gataatgaaa aaggaaagga actccttgct
      541 ctttggcgta aaagtcaaga agacttactg cctttgtttg gcctttcaga acaggaaatt
      601 aaagatatct tggataaggt tttagctttg gatgcaaaat tggcacaata tgtcttatct
      661 cgcgaagaga gttcggaata tgttaaactt taccatcctt ataactggga agatttcaca
      721 aaattagcac ctgaacttcc attagatgct attttccaaa agatattagg acaaaaacca
      781 gataaagtta ttgttcctga agaacgtttc tggacagaat ttgcatctga ctattactca
      841 gaatctaatt gggaattatt aaaggcagat cttattttat cagctgcgaa tgcctataat
      901 gcttacttaa ctgatgatat tcgtatcaaa tcgggagttt acagtcgagc tttatcaggc
      961 acaccacaag caatggataa gaaaaaagca gcttactacc ttgcttcagg accttacaat
     1021 caagctctag gtttgtggta tgcaggggaa aaattctctc cagaagctaa agctgatgtt
     1081 gagcataaga ttgcaacgat gattgatgtc tataagtcac gcttagaaaa ggcggattgg
     1141 cttgcacagt caacacggga aaaagctatt gtgaaactta atgttatcac gccacacatt
     1201 ggataccctg agaaactacc agagacttat actaaaaaaa tcattgatcc taaactatcg
     1261 cttgtcgaaa atgctactaa tttagacaaa atttcaatag cctatggttg gagtaaatgg
     1321 aataaaccag ttgatagaag tgaatggcat atgcctgccc acatggtcaa tgcgtattat
     1381 gatccacaac aaaaccaaat tgtcttccca gcagccattt tacaagcacc attttatgct
     1441 ttagagcaaa gttcatcagc aaattatggg ggaattggtg cagtgattgc acatgaaata
     1501 tcacacgctt ttgacactaa tggagcttca tttgatgaac atggaagttt gaataattgg
     1561 tggactgatg aagattttaa agctttcaaa aaacttacag ataaagtagt tgagcagttt
     1621 gatggactgg aatcttatgg cgcaaaagtc aatggtaaac taacagtatc agaaaatgtt
     1681 gcagatttag gtggagttgc ttgtgcttta gaagctgcgc agcgtgagtc agatttttca
     1741 gcgagggatt tctttattaa ctttgcgaca atttggcgta tgaaggcacg tgatgagtat
     1801 atgcaaatgt tagctagtgt agatgttcac gccccagcac aatggcgaac taatattaca
     1861 gtaacgaatt ttgaagaatt ccataaagag tttgatgtta aagatggtga taacatgtgg
     1921 cgtccagttg aaaaacgtgt tattatttgg taactctaaa tgataaactc ccgttactca
     1981 gatgagtagc aggagtattt ttttattctt ttatagataa agataatgta gtagtgtcaa
     2041 cgcttatctc agctccaata gtcactgtca aaagaggttg ggtgtgcgca aaatccatat
     2101 cataaataac aggaatttcc ttgaaaatag catgttttga aaggatatat tcaaagactt
     2161 gtggagtcat gccgcattct ttaggaaagc gccccataag aatagcttgt ggatgaggat
     2221 aagcttgtaa aattgcagca aggttgcggt caaattcata aaaattactt tcctcagcct
     2281 cttcaatcaa gaggacatag cgttcaattt tcggtgcata aggggtacct cgaagtaggc
     2341 caaacgtaga gagattacct ccgataatcg taccggaagc ttttccgtga ttataaattt
     2401 tccattctgt tgggaaaaat tggcgtggtt tgctgggatc ataccaggga tcactagacc
     2461 attcttcact aggccaaagt tcatagtggt tttctgtcat ggcagtaagc caagcttgtg
     2521 tttggtaagg ttgaccttcc ttcattttga aagaagaata agcaggaccc atgtaagttt
     2581 ggatttttgc tttggcaaaa atagcattaa ggaaggcggt tgaatctgag taaccacaaa
     2641 ttattttggg attcttagag attaaatcat aatctaaata tggtaaaagc tcgttggaat
     2701 tgaatccgcc aatggttgct aatatggcat caacagaagg gtcggcaaat gcttcatgta
     2761 aatcttctac gcggctagca atcgaagcag aatagaggat atcattttct agatagtgct
     2821 tagagaatga cacttgaaat cccagctttt ctagtacttc tttggctaat aggttagcct
     2881 caaaaccacc cagacgttca atggaagaag agggactaag aacacgaatg tggctgcctt
     2941 ttgttaattt tttcattggt taacctccta tttaaattgt tgttagtcta ccacatatgg
     3001 agataatgag caacgatatt cttaaattct atcaatttaa gaaatgcgtg ttatactaga
     3061 gatagccgat gaaagaaaaa atacttatta ttaataattt ggttattaaa taggaggtca
     3121 tgatgtctca attatctttg gaagaacgat tgaatcgact aaaagataaa tacctttctt
     3181 ctaaaaatca agaattttca aatgttcact taagtaaagt aaagaagaaa aagaagaaat
     3241 taataatact tgaaaaagaa agatgtcgct gtcgattgga gagagaagat actaaaaaaa
     3301 tcgatgataa aattgagaaa ttgaagaaag aattgataga tgttatgaag gaggaataat
     3361 atggaagaaa ctattctaat agtcagcttt ctcctgtttt tgattttatc taatgttatc
     3421 aataggatat ttcctaaatt accattacca tttatccagt tggtctttgg gattctctct
     3481 ggcttagttt ttcataagtc acaagtccat atcgatcctg agttatttct tgcctttgtt
     3541 attgctcctt tgaactttag ggaaggtcaa gaaagtgata ttggtagttt tgtaaagtat
     3601 cggactatta ttctttactt aatcttacca acggtatttt taacagctat tgtcgttggt
     3661 tatgtagcag ggcacctttt gccagttagt ttaccacttg cggcatgctt tgcgcttgga
     3721 gcagccttag gcccaacaga tgccgttgcc tttatatcga ttgctaagag gtttcaattt
     3781 cctaaacgag cagagaatat cctcaaattg gaaggtcttt taaatgacgc tagtggtcta
     3841 gtttccttcc agtttgcttt aacagccctt gtaacaggtt atttctcctt agcaaaagct
     3901 agtctcaagt tagcattggc cataatgggt ggttttttaa ttggcttgct ttttgctttt
     3961 ctaatgaggt tatgtctcac tgtcctagag aaatttgatg ctgctgatgt tacaggggct
     4021 ctacttttgg aattaacctt accttttgtt gcttatttcg ttgctgattt gttgggattt
     4081 tcagctatta ttgcggtggt agtggcagga gtaatgcaag caaatcgttt gaaaaaagtg
     4141 actctctttg atgctcaagt agatcgcgtt acctctgtta tttgggaaac cttaaatttt
     4201 attttaaatg gtttagtttt cttaatattt ggaagagaac tgatacgtat cattggtcct
     4261 cttttaacaa gtaacgctta tagtaatttt gatcttatta gtattgttgt tttagttact
     4321 tgcacacttt ttctagttcg ctttctagca gtttctcgct tttatgcttg gagatctttc
     4381 aaataccata aatcatttaa aaagtattgg agagagattc aactgttgac tttttcagga
     4441 gttaaaggaa gtgtttccat agcaacaata ctcttattac ccaagcactc agtcatagga
     4501 gagttggggt atagtttgat tcttttcact gttggtgcag taacgttaat gagtttttta
     4561 acgggacttt tagtattacc aaagttagcg cctcccttac aggtgaaaga tgattattta
     4621 atacgtctat cgattttaac aaaagtatta tctgtcttag aagaagacgg taagagttct
     4681 gaaaatcaag cttcatttta tgcagttata gataattata atagccgtat tcgtcatctc
     4741 attttagaac aagaatctag tgacattaag aaggacttag ctgaattgca actgatgatg
     4801 cttagtatag aaagcgacgg gcttgaagct gcttatcgtt atggcaatat ttctatcaaa
     4861 gaatatcgta tttaccagcg ttatttaaaa tacttagaac gtcaagttaa tcgtagcttt
     4921 gtgtcaaata tcacttatag tttaacgatt cttatgcgtg ttattcgtgg tattgttcat
     4981 gagtttttaa catttggaca aggaatacga aatcggaaaa aacatttcaa aaacaggaaa
     5041 cggttgttag cagaagtcaa tcgtcagcac ttaacagatt tgtacttatc aaataatgac
     5101 ctcattttag aaacgctaga agatatggaa ggttattata atgcaacttt gatagatttc
     5161 ttaaaaaatg aacgcattcg agacgcagaa ttaattaaat caggtttatt tgtagaacgt
     5221 gtcattgcac gcataacgcc agatatttta aatgaaaccc taagaggata ttacttggaa
     5281 cgcagaacaa tctctgaatt tgaaagggac ggtttaatta gttctaaaga gtctcaaatt
     5341 ttaagggata atgtcaatga attagaaagc tattcattaa gagatagtca atcttctata
     5401 ccttatcaat tattggattt aaaaaaataa ttctgataac aaaaaaatca acgaacaatc
     5461 gttgattttt ttgttatctt tggataaaat cttttatatt taaggcggta gacggatttg
     5521 aaaaccttgg tttatcaagg tttttggttg tttcgcccca aattcgcccc aaattatgcg
     5581 actaaaaaaa tatttttgat tttctcaaaa tttctctcag ccagtgcttc catctgatgt
     5641 gtataaactt tcagagtaat atctggactt tcatgtccaa ggagcttaga aattgtcaca
     5701 atgtcaaccc cgttcaaaat caaaaacgaa gcgtatgtgt gccgtaatga gtgatttctg
     5761 actttcctac ccacaatttt cttaatcagt ttattgcaag ctgaattaga tacaccaaag
     5821 cacactctat tcttgatatt agcttgccaa tgatttttct tatattctcg aagaatatct
     5881 attgtatttg aatcaattgg aacttttctt tttgatgact cattcttcag atctgcaaaa
     5941 tcttgagtat tcgagtaatc aaaactcttg tttatatcca aaatgccatt tttgaagtct
     6001 atatcgctcc aggttagtcc catgacctct gagaaccgca aaccagtgac agaaagaagg
     6061 tagagagtga aataggatac atactggata ttctctcttg tgagggataa aagagcctta
     6121 tactcacttt cttctaagaa gtcattttcc tcagctcttg attcaatttg tgatttaacc
     6181 tttgcatcat cagcaaagtt aaaatgaata acttgttccc tcacagctac tttcatagct
     6241 cccttgattt ggtagtggaa cttctcaaga gtttcttgag catatttctc cccaaattca
     6301 tttagccgtt tttgataata gagtggtgtt atatccttaa cctttatatc tttaaaatag
     6361 gttttgatgt gtctaaagtt ctttgtgtag gtttcccagg tcttatcctt tacataagga
     6421 cgtttataaa cttctgacca agttttgaca aaatcatata gagtaacttc tccatcggtc
     6481 aggatatttt gagatagttt tttctcaata tccctagcag ctgcttgagc aagttttttg
     6541 gttttgaaac cactcttaga cttttgacgg aacttaccgc tctcatcttt atatgaaatg
     6601 cgatattccc agccattatc acgttttcga taataagcca tttgtttttc acctcatttt
     6661 ttgataaaat gggtatagta aagagaccta ctgctaaagc aggtttctca ctatccacaa
     6721 ttgccttacg cttgcaccga ccaaagttga gcgtagggct ttttttattt tttaacttta
     6781 tttttcaacg cattctcgat agcattttta agctcaagaa tagctatttt ctcatcttta
     6841 ctgaaggtta ctgtattttc gtcttttact gcatcgaaaa caccgccctt agtatcagat
     6901 gaaccaggat aaaccaattg tagatagcca acagttgaac taggttcctt gagttgataa
     6961 gctgtaattt ctgataatag aattgacttt tctccatcta agccatgtag caacatattt
     7021 gacatgttgc tttttcttgc gattctgatg aagtaatcat caatacgtac aattgttttt
     7081 gattttttga attcaaaaac acgttcgtta ggctctgatg taaagagttc tacttcaatt
     7141 tctttatctt ttccgccaaa taatgccatt attgttgtct ccttttgttt ttattgtaac
     7201 cgtaaaatgt taaaaatctg ccctccacct tgacggtagt ttaaaactaa cttgagggcg
     7261 tagctttcag catactgcaa gttatagatg agatagttca tcagtctgtt gtgtagtgct
     7321 ggtttgctaa tatctgtttc acgttgaata tcttcaaagc tctctctagt tgtgagggct
     7381 tttttgagtc gttcatctgt taggtagagg attgaagcca ctgtatcagc ttctttctca
     7441 attggaatca gctctttcgg gtacttctca ctagcgtttt tactcatgag agacatatag
     7501 attggctgat tttctgtttg attaaggtga cagtgtatgt gactaagttc gtgtaaaatc
     7561 gtgaagataa cacgtccttt ggtgtttgta tgttgattga tatagatgat gtagcgtcca
     7621 aggtctaggt cagggacagt caatccagca cagctttcgc agagtaccct atcagtataa
     7681 gtaacggttc tatttgatac cagccctctg tacttgatgt ctttatctgt gggatgataa
     7741 tcaggtaatt ctggataacg ttctttcatc tcatcgtaat caagaaagtt aaagagaata
     7801 ggatagtttt gttcaaaatg tttaatgaca tcttgataac taacgttctc tttcatttta
     7861 tcaattgagc ttattatttg ataagcctta ttgtgatagt caaaatactg ttcccttgat
     7921 agcggtgtgt atttcaataa gtcacctact tccagttaga atcatcttga atcaattgtc
     7981 tagctgtctt cattagacct tcaagagcag aattgaaccg ctctttttca gaagctgaaa
     8041 gtccttctgt ttccttacga aaaagaacaa tagcttgctg agttagatca tcaactgcag
     8101 tattatcttg gccatcggca atcttaggat tttctgttct accgagaagg tagtcagtgc
     8161 tgacattgaa ataatctgct atttttgaga cacgctcaac atttggtgta gattttttca
     8221 tgttgtatat ggtatttcta ggaaaatcca actgttcttc aagttgattg agtgaaatac
     8281 cttgcttgtc agccaattcc ttgatctttt caaatgtaaa aaacattgat ttatcaacct
     8341 ttctaaggca tgacaaaaaa tatttaataa atttactaca aaactgttga caggttttaa
     8401 taaatttact acaatatcgt ttgtaagcta agtcgttagc gaacaagaca agtaaaaaat
     8461 aaagcctaaa caactgattg gcgtcggttt tatgggttaa gccttagctt tttagcaggt
     8521 cttttctcta tgctttgatt ttaataaatt tatttattgt tgtcaagaag ttcgctaact
     8581 ttttagcaaa aataataaaa ggagggtaga tgaatgggaa tcggttcaat gacagtgaca
     8641 gttaaagtta ctaacatgga taaatttatt gaacttagta aagagttcaa taaaaaagct
     8701 cgcgagctag aagaactagc tcacgaactg aagtcatttg attttgaagg cgaagttgta
     8761 tcaactgata gcaactagct caaatgattc atcatctgaa aaaagataaa cagggtattc
     8821 ttctttagtt gcaaaattga agaaatcata tccaaaatta tcttttttga tgtgggaatt
     8881 tttttcggag agagagtcaa gattgacctg agatagtagc ttaaagattt gcacacgagc
     8941 ttgttcagca tcgttaactc tttgcaaggc tctcaagtgc tcttcgctag ggaaagctgc
     9001 tatcaaatca gctctgtcat ctatgtaagt tctagtgtca tcaagatagc catattttag
     9061 attatagtct acaaatgacc gctgttcata ttcttgatag gtttcaaagc tgacaaggtt
     9121 acctttgtaa cgatagacga gaacgtcatc ttgtaactct tcttcgaaat ttgtgatgcc
     9181 aattagaaat ttatggcatg cctctatgat acgaatttga ttcaaattca tggcttgacc
     9241 tcctttcata aaagatttca agtcaattat accaaactag aaaggaaaaa tatgagtcaa
     9301 caacagaaaa aatggattca gctagtgaaa gataagctga atgaagagca aatgacacag
     9361 acacatctag cccgtgcttg tggagtcgca aaagcgacaa tctcagagct actaaaatac
     9421 ggaaaaggta gtgtccgtct gaaaaataaa gtctcagaca ttcttcacat tgatgaaagc
     9481 tggacggact tggaggaaga ttgatgccga taacaagaga aatgacaatc actgaaatca
     9541 atgttctcaa tgctatcaag aatagcgcta cttatgatct tccgattcaa gccagagagc
     9601 taaggcagca gctaggactt agcaaacgat cacttgaagc agtcattgag aacctgagag
     9661 tgatttacaa gcaaccaatc gttgccaaaa aaaaacagcc tagcggatac tatcttccaa
     9721 gaaatgaaga agaaagaaac gacggcttag ccccatacaa aagacagata ttgactgagc
     9781 aaaataatct tgctgctatt atgtctgtca atctggaaga atattgggca acagaaaaag
     9841 cctgacggca atcaggctca caaaatatta ttacaagagg attatatcac aatgaacgaa
     9901 gtttggaaag atataccaat tgttaaagga tattatcaag tttccaatct tggtcgtgtt
     9961 cggtctattg gtagaacagt caatgcaaaa cagagaacaa gaaaaacaaa aggacgcatc
    10021 ttaaaacaga gcttatcttc aggctatgca atagtgacac tttcagtcaa tggattaagg
    10081 aagtcgataa gagttcatag actagtagct gaagcattta taccaaatcc aatcaataag
    10141 cgaacgataa atcatattga tgaaaataaa cttaataata gagtagataa tttagaatgg
    10201 gcgacagata aggaaaatgc taatcatggg aatagaacaa cgaaatcatc tttaggtcgt
    10261 tgtaaacctg tagaacaatt tacacttgaa ggggaattca taaacacgtt tgactcaata
    10321 aaatctgcat caatgaaaac aggtatctcg tcacaaagaa taacagcaac agcaatgggg
    10381 caccaaaaac agactcacgg atataaatgg aggtacgcat aatgaatgat ctaatgattc
    10441 aaatgcttga ccagttcgaa gctggtctaa tggacagaac gatgaaagta atgcacgttg
    10501 tgacagacga aaagtataga tttcctttag aactcaacaa gtctcaatgc gctttaatgc
    10561 tcttaggtac taaagatact gggacatttg atgaacgctt taactgtcat aaggattttc
    10621 cgaggattcc aaatgctcgt gagaaatacc ctcgtgatga agtcattgaa tggtatcaca
    10681 agaattggca aagaactgtt atctaagaaa gaacaaaagg aactatatca tgaacaattt
    10741 acaaattatc gcagtaggca cacttatttc agtaaccctg attgagtctc ttatctcaaa
    10801 tatcaagctc agaaaagctc tcaagaacaa acagacagct gggactgtca ttgctcaata
    10861 tatcccacaa cgaggcttca ttgactacaa gactggaaga cgtgttgaca ttgaccctga
    10921 aacaaagaaa gaagtctttg ttgactgagg aaagctatgg atgaattaaa agtattgcct
    10981 cacgatatcc aggctgagca gtcggttctt ggttcaattt ttatcaaacc tgaaaaaatg
    11041 attgaggtgg ctgagtatct gaaaccaaat gacttttaca ggccagctca caagattcta
    11101 tttaaggcca tggtgagcct tgctgatcgt ggcgaggcca tcgatattgt cacaatcaag
    11161 tcaacgctgg aaagtacgga tgaacttggc atggttggcg gtatcagcta catcgctgag
    11221 attgttaatg cagtgccaac cagctcacac gctgagcatt atgccaagat tgttgctaaa
    11281 aaagcacagc tacggtctat cattgacaac ttgtctgact caattggcaa tgcctatgat
    11341 gaggacatgg atattgatga gattatcgca aaagctgagc gatcacttat cgaagttagt
    11401 caagccagta acaagagtag tttcagacct atccatgatg tccttttgga aaatcattca
    11461 aagattgagg aacgctcaaa caataccagt caaatcactg gtattgaaac aggattttat
    11521 gactttgaca agctgataac aggcttgcat gaagaccagc taattgtctt agcggcacgg
    11581 ccagcgatgg ggaagacagc cctagctctt aacatagctc agaatgtggc aactaagtcc
    11641 aataaggctg tggccgtctt ctcgcttgaa atgggggctg aaagccttgt tgagcggatg
    11701 ttgtcagctg aaggaacaat catcaaccat catattagaa caggaaattt gacggttaat
    11761 gagtggcaac gtcttatcta tgcacagggt cagcttgctg aagctccaat ctttatagat
    11821 gacacagctg gagtcaagat tactgacatc agagcaagag ctaggaggct atctcaagag
    11881 actgacggac ttggattgat agtcattgac tatcttcaac tcattcaagg gtcacggtct
    11941 gacaaccgac agcaagaggt atctgagatt tctaggcagt taaagattat tgccaaggaa
    12001 ttaaaagtgc cagtcattgc actatcacag cttagccgtg gggttgagca aagaaacgac
    12061 aaaaggccta tcatgtccga cttaagggaa tcaggcagca ttgagcaaga cgctgatatt
    12121 gtcgccttcc tctatcgtga tgcttattac caagacaaaa aagaaggtca gccagaaaac
    12181 gacatcactg agctgatcat cagaaagaac agacacggaa accttggcac agtcaagctg
    12241 tatttccaca aggaatatac caagttttct agcgttgagg aggaatgaaa tgaaaaaatt
    12301 tgaccactat ttcagcaacc atattgctgt aaaaaacgaa tctgatacag tcaccattgt
    12361 tgactattac aatccagaaa agggctatga gtataactta cgttacattt tcgatagaaa
    12421 aaattcatcc cttgctatca caggagactt tggggaatta gttgcacgaa atttctacaa
    12481 tatgggtgaa tgggacacat tctacaatca ctatactggt catctggggt attttcttga
    12541 aaagttacta tgttcaagta ggccagttca ttactatgat actgacaagg ctaaaagaga
    12601 aattctcaat gaattctttg atggtgctga ttatgatgat ttagattttg atgatgaatt
    12661 cctacttgat caattatttg acaattttga cgaagataaa ggatttactt atattcttcc
    12721 agaggctttt gaacgtttta gcgaagatat ggaagaatat gaggtctatg aatttctatc
    12781 aagggctggc cgatacgtca atgatgtctt tgaactttac ttagacgctt ataagagagc
    12841 ttatgagtat ctaacaaagg aggttgatga tggcacaacg cagaatgttt agtagaaaaa
    12901 tcactgagac tgaccgtttt ttggaaatgc cactatcatc ccaagctctc tatttccatt
    12961 tgaacatggg agctgatgat gagggcttta tagataaggc aaagacgatt cagagaacta
    13021 ttggcgctag tgatgatgat atgaaattac tgattgcaaa agggttcttg attccctttg
    13081 atagtggtgt agtcgttatc cgtcattggc ggattcacaa ctatatccag tctgaccgct
    13141 ttcaatcaac cttataccag tcagaaaagg ctcagctaga gtatgacaag tcaaaaacat
    13201 ctagtcttaa acctattgga aattgtatac aaaatgtatc caaaatggag acacaggtta
    13261 ggttaagtaa gggtagctta gataaggata gcttaactac ctatcctaca gtttcagaca
    13321 atgaggaaga agatattcca tacaaggaaa ttatttctta cttgaatgaa aaagctaata
    13381 gaaattacag acctaacatt cagaaaaaca aaactcttat caaggctaga tggtcagaag
    13441 gattcaggtt agatgatttc aaacatgtga ttgataccac ggtcaaggat tggtcaggca
    13501 ctaagtatga aaagtatctc agacctgaaa cgctctttgg ttctaagttt gaaggctatt
    13561 tgaatcaagc accacgaatc aagacggaaa caattgacgg aaggttgggc ttttagatga
    13621 atccttttaa gaattttgaa actaggcaag tcttggaaga aacctgcgag gttcacggtt
    13681 gccagctctg gctgactaaa gtaccgatta agggacggct tgaagaactc aagcagtgtc
    13741 cagagtgtac caaggccgct atcaacatct ttgaaaataa gttgaacagt cagagcaaaa
    13801 tcaacagcaa gcttgctgat acatatgctg tctttgaaag agatagcttg gtatcagaca
    13861 agctaagagg caagagcctt gagaactatg aaatcaagga tgatattgac cagcaagcta
    13921 tcaattatgc taagcgtatg gaacagttct accgacagga tagaacaggc aatgccattg
    13981 taactggccc ttctggtgtt ggaaaaagtc atctgactta cggcttggcc aagtttatga
    14041 acgaacaatt caaagcctac gaatcaccaa aatcagtcct cttcatctca ctggtgagcc
    14101 tttttaccaa aatcagagaa agcttcaaag ctgacaatgg gtaccgacag gctgagatga
    14161 ttgagctgct aactagtgtg gactacctct ttctggacga tctaggcaag gagagtcgca
    14221 aaggcgacag ccagaacaat gagtggactc atcagattct ctatgagatt ttggacaatc
    14281 ggagtaacac aatcatcaac acgaatctga gcagtaagga gattaaggcc ttatatgctg
    14341 acaattacgg taatggtgct ctttctagtc ggattcttga gggagtgact ggcaatagct
    14401 ttgcttaccc taaggatatg gaagatagga ggtattgatg agctataaag ccacattcat
    14461 gctatatggt tacaaagctg ttccgattgg tctatttgat acaaaggagt tggccgctca
    14521 agcaattgtt gaccacatca ttgaaaactc tggaatacct agacccaggg tagttgccac
    14581 tatgaaaatc cgaagcaagg aaatcagaat tgattatggt tctaaaactt gctactactt
    14641 gatcacttta attaataata aaacggagga aaaacaacat gactaaaaca atgacacttg
    14701 aagaaaaggt tgaacagtgg tttattgacc gtaacttaca tgaggccaat cctgttaagc
    14761 agtttcagaa gatgatggag gaaactgggg agctttactc tggtattgct aaggacaaga
    14821 gtgaaattat ctatgattcg cttggagata tgcaagttgt cctgattggc attgaacaac
    14881 aaatcaagaa tggcgctcag attgaagcaa gtccacaaga tatggagttg ttgctattgg
    14941 cttcaagttt gggggaaatg gcacagaaac tctacaagca tatcttccac aatgaaacaa
    15001 aaacaccgct tatccgtcca gaattaagct tgcttcactc tactattcat gcaatagcta
    15061 tccacaactt gacaactgct gatgactgcc tatcaatcgc ttatgaagaa atcaaagaca
    15121 gaaaaggcaa gttgatcaat ggcatttggg ttaaggagga ggatttgaaa tgaaaaacat
    15181 gaattggaaa gcattcttca acaatctcaa ggcctttgca agtgtcatca tcattactgt
    15241 atttgttctt ggattcaact atctgtttta tgagattggc taccagaaag gccatcagga
    15301 agctgacagg gtgattatct atgtcgctga taatgctggc gctgagatgt ttggcaaaat
    15361 cactgataaa gaaatcattg aaggccgtca cacggttaca gctggcgctt atggaaaatt
    15421 tctggtcact gaggaacaat acaatgaaat caccgttggt gatgacatac cagactacct
    15481 gaaaggacgt ggaaactaat gacaaaatgt aattttgaag caggacacaa accaattgac
    15541 acaattggac aactagccaa agaagtcctt gatgggaaat atggcgatgg tgaggaacga
    15601 agaagaaatc taggctgttt atacgatcta gttcaaacaa gagttaatta tatggttcat
    15661 gaacgtgaca gcaagttcac aggtatcaag atttacgaca atgtgaacaa acctagccat
    15721 tatcaaggaa agaatggcat ggaagccatc gatgttgtca gaaatttcat tggacagctc
    15781 acaggagtat ctgctttcta ctggggaaac tcaatgaagt acctgttacg cttccagggc
    15841 aagaacggcc ttgaagacct caaaaaagca agaaagaacc ttgactggtt gattgaagaa
    15901 atggagaaag agaaatgaaa aaatcaatag gaatccttga atttaaagaa tttgatgctt
    15961 ttgttggtgg aaaggttatg cctgtctatc ttcatagtgc taacccaaac gtgccaacga
    16021 ctaataaaaa tgaggctatg agggttacca aaaagagata cagaaaagag ttctctgaca
    16081 agtacatttt cagaaaggtt ggtgaggttg atgatcaata atgttgtact tattggccgc
    16141 ttgacaagag atgttgagct acgttacacg ccgtcaaaca tcgcaaacgc tacttttaac
    16201 ctagcagtca atcgaaattt caagaatgct gctggtgatc gtgaagctga tttcatcaac
    16261 tgtgtgatgt ggcgacaaca agctgaaaac ttggccaatt ggacgaaaaa aggaatgctg
    16321 attggtatta ctggacgaat ccagacaaga agctacgaaa atcagcaagg tcagcgtatc
    16381 tatgtgactg aggttgttgc tgacagcttc cagattcttg aaaagcgtga taattcaacg
    16441 aaccaggcaa gcatggatga ccaattgcca ccatcatttg gaaatagtca gcctatggat
    16501 atttcagatg atgatctacc gttttaggag gtgcttatga acgaatataa gaaacccacc
    16561 tacatcgtca ttcaagaggc aatggctgag cgtattagat ttcttgaaga tgaattatat
    16621 aacagagcat acaaagacat tgaacggcta gaagttgaga atgaccgtct gaaggttaga
    16681 tgccttgatt tgcagttgga aaatgctgac tatgtctggg atgacatgtg tcgctctgct
    16741 attgctcgtg caaaatcaga aaatcgaaga ccacacagaa gatggagggg aagatgaagt
    16801 taaggcttga agaactaaga aacaaacttg gcctcagtcg cagagatgtg catgaagcca
    16861 ctggcatttc tatgaacacg ctgctacatt atgaaaaagg cggtacacca tctattggtc
    16921 aggttgaaaa tatcgctaag gcttacaata tcaacccagc ctggttgctt ggttggattg
    16981 atgaaggcca gcaaccacaa ccacttgaaa ccgttgttga aaagattatc tatgtggaaa
    17041 aggaaggctg tagattaccg ccatattgga acaatgataa taatggcaga atcatcaagt
    17101 ggaaagaatc aagaagagca ttccaaagga gaacaatttg acaaagaaaa cagcaagtaa
    17161 gacaagacgt gatttccttg agtttgagct tgaggctaag tatctaaaaa ttgataaact
    17221 tattgggcag cgccgtcatg aactggaaag aatctatgca gtcaaaaacc tgacaatgcc
    17281 aggaatcgat gattcaggag ctagtcgcag tggaacttca actaatactt ctgaaaatct
    17341 agctgttgct tatgccagtg acccaatgat tctgaaactg gaagagttcc aaaatgcgat
    17401 atcagaatta ctagaggtac ttgaacccga tgataagaaa attttccatc tgaggtgggg
    17461 agaacatact ggatatgatt ggattcaagt ttggcacatc atggaaaatg gcgagacagg
    17521 ttatctttac agacatagca agcagattta tagaagacgt gaagtcatcc ttgatacact
    17581 tgcaaaatta ttgttcatgt aacttgtcaa aaaaacatat agaattgaca gaaacaatct
    17641 gatagattga tagtgtcgct aagcaccgag aaatccttgg tgctttattt ttttgagaaa
    17701 ggagcaaagc gatgaatatt gttgaaccgc tacgagacaa agacgatata caagccatga
    17761 aggactatct atcatcttgg aatgaaaagt attacatgct atttcttttg ggcatcaata
    17821 caggtttccg tgttggagat attctcaagc taaaggtcaa agatgtccaa ggctggcaca
    17881 tcaaagttag ggaacaaaag actggtaagt ataaaagtat taagatgaca aggccactca
    17941 aaaacgaatt gagggaattt gtcaaagaca aggaacttca tgagtatcta tttcagagtc
    18001 gtgtcggaaa gaacaaggcg ctcagttata agacggttta ctggtttctt aaaagagctg
    18061 ctgaagacct cggcattgac aatgttggaa ctcatacaat gcgaaaaacc tttggctatc
    18121 attactacaa gaagtacaag aacgttgctg acttgatgtc actattcaat cattcaagcc
    18181 cagcagttac actaatctac atttgtgtaa ggcaagatga gcttgatact aagatgagta
    18241 attttagcct ctaatatttt tttgattttt tcaactatcc ataacgagga agtttctagt
    18301 ctatattttg aagtgagctt aaagccttgt cctaattagt tttctagtgt gaaacaaaat
    18361 tggataaaat ataagatata gatagttcag catggttatt ttacataatt tcagccatag
    18421 aaaaataatc ttgtcaaaaa aatgggtatt attgacaaaa acaatctgat atatttgtaa
    18481 catgagaaaa atccagagag caagcttagg catgttcttt tttttgttgg gggtgacgat
    18541 gccatgaaag atgaaaatgc atttgatgga attaggcttg aagctggtag cactttagaa
    18601 atctatctca cgaagaacga cttagaacat attgcaaatg gatatgaggt gactttagat
    18661 attaagccta acgagactgt aaataaaatc gtaataaagc caagttttgt aaataatatt
    18721 ctaaatccgt taatcaacta tgacaaaaga atagtgagtg aagcagattt aaagtttaga
    18781 gacatttcaa gagaggttgc aagagatagt tttgcattgg ggtcaatgta gtgggctata
    18841 gaaaccctaa acactctgac tggttcagag cttggcagat taaattctac aactcgaaac
    18901 cttggagaac tctgagaaat aaaatcagaa aagctaagcg tatgcgttgt gacatgtgcg
    18961 gacgtttgat tcacggtaag agtattgttg accacatcat agagattgac gaaactaatt
    19021 atcaagatga atctatcact ctcaacgaag agaactgtca gttactttgt cttgaatgtc
    19081 acaacacaaa aacttttcaa agtaaaataa atttaaattt agataatcgg aatataaatt
    19141 tatttttgat ttttttattt ttgaattttt gtggactccc cccattttga attttgacag
    19201 cgccaaaata ataacggtgt caatcctctt gtgtacctct cccccaaaat tgacgaaaat
    19261 tgatacaaga aaggagcatg attttgaaaa tcaatgaagt tttagaaaag ctaggaataa
    19321 gtcgtgctac ccttaccagg tatcgaaaaa agctgggcat atttgaagaa actaggtcaa
    19381 atatcaccaa aagtcagttc aaagagttgg aaaagcttgc caatcaacgg caaaagtaca
    19441 caaggcagga acgtgttgag ctatctcgta agactttcaa gctgattcca aaagaaaaaa
    19501 tgcttgaaat cagtgataat gattcagttg tgttgaaaaa tctcaaaact caatacaatc
    19561 acaatcaaaa agtgattgaa aacttccagc ttgaaatcaa taaggtcatc aatgacggtg
    19621 agctacctga taagtaccta cttgatggaa tggaaaagta tcaaaagctt aacatgcaga
    19681 tcatgtcaac gattgaaaag caaagtccac agggtgacag tctcaaagaa atgattcagg
    19741 agaagttagc acgttatggt tgagatgaga tattttgata agtatgctca gctcatctat
    19801 actggtaaga ttcgtatttg taagctcaca atgaaatcaa ttagacgtgt tgagcgatac
    19861 aaagagcaat acctcttcaa acaggaggaa gctgacaaac ggattgagtt cattgaggaa
    19921 gagtgcagca atactaaagg ccttgctggt aagttacgct tagcattacc acaaaaggtt
    19981 tggttagaaa caacgtgggg cttttatcac acggttgagg ttactaagac caatcctgat
    20041 accttggaag aatacacaga ttatgaagaa aggcgtctca ttcatgaggt gcctattatt
    20101 gtgcctcgtg gcacaggtaa gactactctt ggttctgcta ttgctgaggt tggtcaaatc
    20161 attgacggtg agtggggtgc tgatattcag cttcttgctt acagtcgtga acaggctggc
    20221 tatttgttca atgcctcaag ggcgatgttg tcgaatgaag aaagcttgct gcactatatg
    20281 cgtgaggctg acatcctacg gtcaaccaag caaggtatct tgtatgaaac aactaacagt
    20341 cttatgtcta tcaagacttc tgactatgaa agccttgacg gtactaatgc tcactacaat
    20401 atctttgatg aggtgcacac ttatgatgat gacttcatca aggttgtgaa tgatggttcc
    20461 agccgtaagc gtaagaattg gataacctgg tacatttcca caaatggaac taagcgtgac
    20521 aagctctttg ataagtatta caacatctgg gtagatatcc ttgatgacaa gattatcaat
    20581 gattctgtca tgccttggat ttatcagttg gacgatgtgt cagagattca tgaccctgat
    20641 atgtggcaga aagctatgcc attacttggt atcacgacag agaaagaaac catcgctcgt
    20701 gatattgaga tgagcaagaa tgatccagca caacaagctg agctgatggc taagactttc
    20761 aatcttcctg tcaacaacta tcttgcttac ttcagcaatg aagagtgtaa aggttggtca
    20821 gataagtttg atgagagttt gtttgtcgga gatgatgaac ggaacgcccg ttgtgtgatt
    20881 gggattgact tgtcagatgt caatgacatc tgctctatct cttttatggt tgtgcgtggg
    20941 gaagaacggc actatctaaa caagaaattc atgccacggc ataccattga gacattgcca
    21001 aaggaactgc gtgataagta cacggagtgg gaattaagtg gcatgctgca tgtgcatgaa
    21061 ttggactaca atgaccaagc ctatatcttt gaagagttac ggcagtttat gagtgacaac
    21121 agaattttgc ctgtggcagt cggttatgac cgctacaatg caagggaact tattcgcttg
    21181 tttaacgact actacgggga tatttgtcac gatattcccc agacggtcaa atcgttatca
    21241 aatccgctca aggtttacaa ggagaaggct aagatgggca aaatcatctt tgatgatcct
    21301 gtggcgacat ggaatcatgc caacgtccgt gtcaaaattg atgccaataa caatattttt
    21361 ccaaacaagg aaaaggcaaa agaaaagatt gacgtctttg ctagtcagct agatgccttt
    21421 atctgttatg aaaatttcaa ggaagacttg agctactact ttgattgagg tgaagaatga
    21481 acaactattt gaaaaatttg aaggaggttt ttgctaggat tttccgtcca aacaatcgga
    21541 aatctacaag aacctatctt caaagaagta tctcctactg gcgtaggaac tccatctatt
    21601 tggataacat ctacaataag atttcaactg acacagctca gttaaggttt aagcatgtca
    21661 agattactcg taacccaggc ggtgtcgatt cgatggtttg gtatgagcat agtgatttgg
    21721 ctgaggtgct aacagtttca ccaaatccac tagaagtgcc tgttgtcttt tggtcaaatg
    21781 taactagggc catgctgcgt gacggtgtgg cggttgttgt accacgttgg aagaatggcc
    21841 gactggttga aatatggctt gctaagaaga cagtgacttg gacagcagag agcgtggagc
    21901 ttatgcttga tgatgttgct gttgagcttc ctcttactga tgtgtgggta tttgaaaatc
    21961 ctaagttgaa tgtcacggct caactcaatc agattactga gcttattgac attaacttga
    22021 atgctttgac agaaaagctt agcgatggca attctagctt gagaggtttc ttaaagctac
    22081 caactaaggc agctgatgaa catttgaagc aacaagccag ggaccgtgtt gatagtatgc
    22141 ttgacttggc caagaacggt ggcattgctt atcttgagca aggcgaagaa ttccaggaac
    22201 ttagcaaaga ctattctact gcttctaagg aagaattaga gttcttgaaa tcacagcttt
    22261 ataatgctca tggtatcaat gagaaattat ttacctgtga ctatactgag gaacaatata
    22321 gagcctacta ttctagcgtc atgaagttat atcagcgtgt ctattctgag gagattaaca
    22381 gaaaatactt cacgaagaca gcacggactc agggcaataa gttactggtc ttctttgata
    22441 tggctgacat gatttcattt aaggatttgg ttgaaggtgg cttcaagtcc aaatatgctg
    22501 gattgatgaa ctcaaatgag ttccgtgaaa cctatcttgg tttacctggc tatgagggcg
    22561 gtgaagtatt tgaaaccaac ctgaatgctg ttcgtatcgg tgcagaagaa tctgagtaga
    22621 atctaaaggg tgggtggttg gcatatcttt cacgaaagga ggtaagcaat ggaaaaacta
    22681 aaaacctttg ttgtgaagtc ggttgaggat gaatcagctg actttcattt tgaggcttac
    22741 gcttcaacct atgacaatac agacagagaa ggcgatgtga tggccaaggg gtgttttgat
    22801 aacactctga aatctaaggc tgttgtccct atgtgtctta atcatgaccg taactgtgtt
    22861 attggtaagc atgaattgtc tgtggatgaa aaaggcttgc gaacacgttc aacgtttaat
    22921 cttagtgatc cagaggctaa gaaaacttat gacctcatga aaatgggggc attggatagc
    22981 ctgagtattg ggttctttat taaagattat gagcctattg acgctaagca accttatggc
    23041 ggatggattt tcaaggaagt tgaaatcttt gaaatttctg ttgtgaccgt gccagctaac
    23101 cctcaagcaa ctgttgacaa tattaaggaa tttgatatgt cggcggttga taagcgaatc
    23161 gctcaggtga acatgaggca agaaatcatg agtaaacttg caaaaattta aaggagacat
    23221 gatgaaaaaa tcactcattg agcttttgga agctcgtcaa aaagcaactg atgaactggc
    23281 tgaggtaaag cttaaaaaag ccactattga agcaaagatg aaatcttcaa ccattgaaga
    23341 cgatgacttg gaacagttaa aaactgatgc agaaagtttg gtttctcaag caacagctat
    23401 taaggaaaca attgctgggc tggattctga cattgaagaa actgaagaag aactcagcaa
    23461 agctgctaaa atcattaaag aaaaacagaa aggtaataca cctatggatt atttgaaaac
    23521 caaagccgct gctcttgact ttgtccgtat tctgatggac aacgaaggta gcgcaaacag
    23581 tgcccgtaaa gcgtgggaag ctaaccttgt tgaaaaagga gtgactaacc tcactaagat
    23641 tcttccagaa cctgttctta tcgctattca agatgccttt actaactata atgggatctt
    23701 gaaccacgtt tcaaaagatc cacgttatgc ggtgcgtgtt gctcttcaaa ctcaagtatc
    23761 tcaagctaaa ggacacaaag ctggtaagac taagaaagat gaagacttca cattcttgga
    23821 tttcactatc aactctgcga cagtttatat caaatatgcc tttgaatatt ctgacttgaa
    23881 gaaagataca acaggcgctt acttcaacta tgtgatgaaa gaacttgctc aaggcttcat
    23941 ccgaactatt gaacgtgctg ttgtcattgg tgatggaaaa tctaacagtg ctgaagacaa
    24001 aatcactgaa atcaaatcaa ttgctgaaga aactgaagcg aatctctttg aaacacaaga
    24061 aatcaatgtt gctggtgtat ttgacaatgc tgttcttgaa acgctggttg ctggtattga
    24121 taagatggtt ccaaatacta ctccaattct tgtcacttca aaagccattg ctcgtaaatt
    24181 gaaattggtc aaagatgcag aaggtcgcta catcgatcct caaccatttg ctccaattgc
    24241 aaccaacgga aatgttattg ctggttttca agtttacatc tatgactgga tggacggtgc
    24301 aactaaccca atcattgcat ttgctgacca agcttataag atgattggtg atgaggctgc
    24361 cgctgaccgc tttgatgatt atgatgtaac agtcaaccgc cgtcatattg agcttgctag
    24421 tgttatgggt ggtcgtttgg ctcagtacaa atcagcggtt aagtttacaa accaagcagg
    24481 ttaaatatag aaaggggagt ctgaatgaca atccttaaaa caatcaaaga aatggtagaa
    24541 gttgacgttg aagaggatat ctttgacgtc caacttttac gatatatcaa cagtgggatt
    24601 tcatatttac aaagaaatgc tattcctgtt atccgtatcg ataaagaaac ggagttagag
    24661 ggatggacag acattaagga agaagatagg gagacagtgc ttgactggtt acatctgaga
    24721 tgcgtccaac gttttgacaa atctttgatg acaggcagtg ctgctactat ggaatggatt
    24781 gacggagaga tgacaaatct tttgtatcaa ctcaaggcaa tctatgaagt gaagacatga
    24841 aatcttcaag agtagctatc attctttgct atgatgagcg tgttgaggtt gaaaaaggtg
    24901 tctgggagaa aaagaccgta gaaaagaaag tcaaagctaa gaaagagaaa atctatcagc
    24961 gaaggcttga taaagctatg gcagacggtc aagttctgac tgctaggttt agtgttcgtt
    25021 ccaactatgt gactgacact cttgactatg tcaaatataa tggcaaagag tacaaggtta
    25081 acattgggac agaatcagat gatggtcatt acactatcat cgaacttgga gaactgaaat
    25141 aatggccaag aaatttttca ctagacaaga tattcaatct atccttgaaa caaacccttt
    25201 gaaggctaag gtcttctaca tggaacgtga ggagaaatct tctcctgaca atgtcatctt
    25261 gtattatcgg ttaacaccag gcagtagcat tactgctgat gatagagtgc acatgagaaa
    25321 agtgactttg caagttagtc actaccacaa gaagaaacta gacagcattg aaaatttgat
    25381 gctgtctaat tttatgtgtg agccaaatca attgaatcta aaacaacctg acactgacta
    25441 tctactgaca acttacagac ttgaggtgtt tacaaatggg cggtggtagc gtgaagatga
    25501 caccgctaaa ggtggatatc aagaatcaag ttttagagaa cattaagaag gctgcccaaa
    25561 gtacagaaaa tgacataaga accaaaagcc ctagacgaaa cggtgtttac gaaaaaggtt
    25621 ggacacacga catcatagat gacgccgctg ttgttcataa taatggcaaa gaaaaatctc
    25681 tatcccacct attagagaat ggtcatgcca ctaaaaacgg tggctttgtt gcccctagag
    25741 agcatatcag accagcctac atcaagaata aggagaaatt ccttaatgat atgaaatcca
    25801 ttaaaatcac acctaaataa gaaaggagtc ttacatgact tatcaatacg atacaaggga
    25861 agtcactcac ggtaatgcga atggttttta tgccaaaatt gctaaaactg atgctggtgc
    25921 cttagacttg cagaaacctt atccctttac agggctacga agcacatcct ttgaaacttc
    25981 tcaggaatca aatgcctact atgcagacaa tgtggaacat gttcgtttgc aaggtaaaaa
    26041 atccacagaa gggtctatta caacctatca gattcctaaa cagtttatga ttgatcactt
    26101 aggtaaaaag cttacgaact ctacaccacc agctttgatt gatactggtg tcaatactaa
    26161 cttcatttgg ggttatgctg aaactgttac agatgagttt ggagctgaga ttgaagagtt
    26221 ccacatttgg acgaacgtta aggcatctgc acctaaaggc agcacatcaa ctgatgaaac
    26281 atcagcgaca ccaaaagaaa ttgaaatccc ttgtactgcc tctccaaata acttcattgt
    26341 ggattctgaa aagaaacctg tatcagaaat tgtatggcgt gatgacagca aaggaaccgt
    26401 ccgtgggaag tttgacaagt tgtttgctga caagtcacca ggcaagctga ttgatttcat
    26461 caatgaagcg cttggaacaa ccactacacc taccacaact gttagcggag gctcttaatg
    26521 attaaaaaag aactatcatt cacagcgttt gatagttacg gtgaagaaag agagcacact
    26581 gaaacagtgc gctttcttta ctctttacca gctatcaaga tgtatgaaca gcgaactggt
    26641 cgtaacttct ttgatgataa ccaaaaagca cttacagcat atacacaact tgctcttgca
    26701 actggtgtaa acggtaacct atctgattta actgatgaag agaaaatcaa gatgatgcct
    26761 ctacttatgg aacctgattt catgaacttc ttgactgaag tcattccttg cctttatggt
    26821 gaggttgaaa atggacgttt agtacaaaat gagcttaccg ctgaaacagc ttcattagct
    26881 ccttggtttg gtgatttgat tgatattggt ttcttctcag acctcttcta tgaatttaac
    26941 cgaagcagag caaaggtgcc gcaagataaa aaaaagcctt ttcagaagtt gtaacttccg
    27001 aaaaggttta taaggttatc tttgaaaatc ggatggatgt tttttgggca gaatcccaac
    27061 actttaacta tttgatggga acacttcacc aaatgagcat caatcaggaa gagaagaaaa
    27121 ccttatccaa cgctgattta cttagcgtga tgtcagatta aaaagaaagg aggaaatcta
    27181 tgactgaaac gtttgaaggc ttatacgtca aatttggtgc taatactgtt gaatttgata
    27241 ggtctgtaaa aggtatcaac actgccttat ctagtttgaa aaaagacttc aataacatca
    27301 acagacaatt gaagatggat ccagacaatg ttgacttgtt gaatcgtaag ttggttaact
    27361 tgcaagaaca ggctcgtgtt ggtgctatga aaattgctga actcaaaaag caacagaagg
    27421 cactgggaga atctgaagtt gggtcagcac agtggaataa gcttcaactt gaaattgcta
    27481 aggttgaatc acagatgaag attgttgata aggcaatgga gtcaacaaag aaacacattg
    27541 aagatgtagg agacccaaag tctattctga atcttaacaa agaacttgat aatgttgcta
    27601 aagagcttga tattgtcaat caaaagcttg agctagacac tgacaatgtc gaactagcag
    27661 agcaaaaaat gaaactactt ggtaaacagt cggaattggt tggggataaa gtccaagaat
    27721 taaagaaaaa acaagctgcc cttggcgatg agaaaatagg tacagaagaa tggcgtcaac
    27781 ttcaaaatga aatcggtcaa gctgaagttg aagttctaaa gattgaccgt gcaatggaca
    27841 ttcttggtga gtcaagccgt tctgcaactg gagacatcaa agaggcaacc agctatttaa
    27901 gagctgatgt catgatggat gttgcagata aggctggtca gattggccag aaaatggttg
    27961 acgctgggaa aatgacagta gatgcttggt ctgagataga tgaggctctg gacaccgtca
    28021 caaccaaaac tggtctgact ggtgatgcct tagcagagct tcaggaaatt gctaaagaca
    28081 ttgctactgg tatgcctacc agctttcaga atgctggtga tgccgttggg gaattgaata
    28141 ctcagttcgg tttgactggg gaaaagctga aatcagcatc tgaattactt atcaagtatg
    28201 ctgagattaa cgaaacagac atttcaagct ctgccatttc tgcaaaacaa gctattgaag
    28261 cttacggttt gacagctgaa gacttgggaa tggtcttaga caatgtgacc aaagccgctc
    28321 aagatacagg acagtcagtt gacacgattg ttcaaaaagc cattgacggt gctcctcaga
    28381 ttaaaggttt gggactttct tttgaagaag gtgctgcact gatcggtaag tttgagaaaa
    28441 gcggtgtgga ttcatctgct gctctatcct ctctatcgaa agctgctgtc atctatgcta
    28501 aagacggtaa gactctgaca gatggattga atgagactgt tagtgctatt caaaattcta
    28561 ctagtgagac agaggcttta agtattgcct cagaaatctt tggtagtaag gctgctccta
    28621 gaatggtcga tgctattcag cgtggtgctt ttagcttcga tgacttagct gaagcagcta
    28681 aaagttcctc tggtactgtc tccaccacat ttgatgagac gcttgaccca atagataagt
    28741 tgactcagta ttctaaccaa gcaaaagagg gaatggcaga acttggcggt aaattgcttg
    28801 agactgtcat cccagcttta gaacctttga tgggtatgct tgaatcttct gtcaattggt
    28861 ttactagcct aaacgaaact gatcaacaga ctatcgtgat tcttggccta gttacaactg
    28921 ctgtgatgat gttgcttggt gcaattgcac cgctggtcat cgccataggg gcaataggtg
    28981 cgcctgtcgg aattgtagtg gcggcaatag taggggctat tgccgtcata acacttatca
    29041 tccaagcaat catgaactgg ggagccataa ctgaatggct tcagtcaacg tgggattctt
    29101 gtgctgcctg gctttctgaa ttgtggacta acatagtcac gactgccacc acagcgtggt
    29161 caaatttcac tgcctggctt tctggccttt ggtcttcagt agtctcaact ggacagtctt
    29221 tgtggtctag ctttactagt tccttgtcca atattttctc aagtttgatt acaggtgctc
    29281 agtctctgtg gtcaagtttc acttccactc tttccaattt gtggtctgga ctggtctcaa
    29341 ctgggtcaaa tttgtttaat aatttgagta gcacgatttc aggaattttt aatgggatac
    29401 tttcaacagc aagcaatatt tggaattcca taaaatccac tatttccaat gcaatagatg
    29461 gggcgaaaaa tgcagtgtcc aacggggtca atgccatcaa gaatctgttt aacttccaga
    29521 ttaaatggcc tcatattcca ctacctcact tccgtgtgag tggttctgct aaccctctgg
    29581 attggctaaa aggtggctta ccaagtatcg gcattgactg gtatgccaag ggcggtatca
    29641 tgaccaaacc aaccctattt ggcatgaatg gaaaccgtgc aatggttggc ggtgaggctg
    29701 gcgctgaagc catcttgcca ttgaataagt caaccctggg ggcaattggt caaagtattg
    29761 ctaacacgat gaatacatcg aacaatatta acgttaactt ctctggcgtc actatcaggg
    29821 aagaagctga ccttaacaga ctagccaacg tggttggaaa tcgtattgct gaagaattgc
    29881 aacgtaaaac taatttgaga ggaggaatgg catgacaaaa atcaatgaac ttaccattga
    29941 cggtgtgaaa acatcatctt ttaagtgtga tgtattggtt gaaactagac caaatgtcat
    30001 tgtctccagc tccaaaacag ctctattaga gcatgatggt attagtggtg cagttgtgca
    30061 atcgaataga caccgtgggc taattgagaa gccttatcat atcactttaa ttgagccaag
    30121 cgatgaagaa atttatcgct tttctgctct tttgaaccgt gaaaagtttt ggttggaaaa
    30181 tgaacaggaa ccaactatta ggctttggtg ttataaggtt gatagctttg agattggaaa
    30241 agacgaattt ggtgcctggg tggtcgatgt taccttcatc tgccacccta ccaagttttt
    30301 taaaacaact gacacccaga ctttgactgg gaatggggtt ttgagggtgc aagggtcagc
    30361 tcttgctttc ccgaagatta cagtggttgg tcaaagcgct tctgagacat cgtttacggt
    30421 ggggaatcaa gtgattaaac ttgaaaagct ctcagaatcg cttgtgatga ccaacgatcc
    30481 tgacaatcct agctttaaga cggctagtgg caagcttatt aagtgggctg gtgattttat
    30541 cacagttgat actgctaagg ggcagaatgt cggtgttgtt ttaggtgctg gcataacgtc
    30601 cttgaaattt gaaacagttt gggggtgggc atagttgctc tttttacttg ataaagatgt
    30661 tagaacggtc aaatggaatg gcattccact tcacgaggcc agctctgcca ttgtcaaaga
    30721 agaaaccaac agtgatttct acctgactgt tcgctatcct atcacggatt caggtatcta
    30781 tcaacttatc aaagaggata tgctgattaa ggcgcctacg cctgtgctgg gtgctcagct
    30841 gtttagaatc aagaaaccta ttgagaatga tgatagcttg gatatcactg cttatcatgt
    30901 ttctgacgac atcatgaagc ggtctatcac tcctgtaagt gtagttggtc aaggttgtgc
    30961 tatggcactg tctcagatgg ttcaaaatgc taagactggt ctaggtgatt tttcgttcac
    31021 cagcgatatt atggacagca gaaccttcaa cacgacagag acgaaaacac tgtattcagt
    31081 ccttatggac ggtaaacaca gcattgttgg aacgtgggag ggtgagcttg tccgtgacaa
    31141 ttttgctctt tctatcaagc gtagccgtgg ggctgatcgt ggtgttgtca tcacaacaca
    31201 caagaacctc aaatcctatc aacgaaccaa gaactctcag ggtgttgtca caaggattca
    31261 tgcacggtca accttcaaac ctgatggagc tgaagatgaa gtgacgctca gagtgtctgt
    31321 tgacagtcca ctaataaact cttatcccta catcaatgag aaagagtatg agaacaacaa
    31381 cgctgaaacc gttgaagacc ttagaaagtg ggctgaggct aagtttacaa atgagggcat
    31441 tgataaggtt tctgatgcca ttgagattga agcctatgag cttgacggtc aagttgtaaa
    31501 tctgggcgac acggttaacc tcaagagtag aaagcacagt gctgacctct acaagaaggc
    31561 cattgcctac gagtttaacg ctttaacgga agagtatatc tccataactt ttgatgataa
    31621 gccaggagtt ggtggttctg gggtgtcaag tggcttgtct aatgttgctg atgctatact
    31681 tgtagcaagt gccacagctc aggacgttgc tgttcaaagg gctgtgaaaa atgctaacgc
    31741 tgcctttgat gcagagtttg gtaaaacaaa aacaaaaatt aatgatgata tcgaaatcgc
    31801 aaaagccaaa gtagaaagct ttaaatcgga actatctaat cgcatggata atcagcttct
    31861 tccccttgcg acagaagcta agaatttagc cagtcaggca caagcagacc tgaccagaaa
    31921 agaaatagag cttcgagctg aattaaatag acaagtcaca agcacagaag ctgtaaaaat
    31981 ctctctaaca aatcttagtc acaatatgga tatcattaag caaaaagccc taaacgacct
    32041 tagagacgca gagacaaggc taaaggaagc tgatagcgtc caacaacttg cgacaaagcg
    32101 ggttgaggat aaactgacag gacttagcac gaaactggag tcattctctg ttggtggcta
    32161 taattatgtc attgacggcg gagaacctaa agaactaatg gctaacttct atggtaagac
    32221 ctatgatatt aatccccaac tactcgaacg aactagtcaa gcgaccttga gttttagcta
    32281 tgaagcagaa tcaactagtc gtttagaagt tagactttat aaaaagatgc acaccgggga
    32341 cactagtaag attacaatta ttgtcatgcc taactttgac ttatctccag ggaaaggatt
    32401 tatctctcag agctttgatt taggtggtgt tatgcctgac cctagaaacc aagcctggct
    32461 tgtgatgcgg gggacaaatg ctaacccctt gacgctctcg aaagttaagc tggaacgtgg
    32521 taccgttgct acagactgga ataatcgcga tgaaacctta aaagctagct tcgccgaata
    32581 caagcagacc gttgatgaaa acttagccaa ccttagaaca agtacggaaa ccttggcagg
    32641 tcaactgaca agtgctgagt ctagcattag gcagacgtcg gaatccttta gcaatcgttt
    32701 ggttagtctt gagacctata aagatagtga atcaaatcga gctagtcgtt actttgaagc
    32761 aagcaagtct gaaaccgcta agcagctatc agccttacga acagaagtta atggctctta
    32821 tgtggataag tcaacctatg aggaaaatgc aagaggtttg acaagacgct ttgaaagtct
    32881 gtcatcaggg acacagaact atgctttggg gactgaaaga gatgtctatc taaacgtttc
    32941 agggactacc gtctatgacc tttatagctt cggcactacc ttagcaataa aagggattga
    33001 aatgggagat actgtaacta ttgcatttaa ctattttgcg gatagtcgga cggcttttgg
    33061 ttcttatgaa ctggagctat acggacatgc aggcaaaatc gaacgggttg gtgaggtaca
    33121 gagaacgata tcaaatcgtg gtacctatac cgcatctttt gtggcaaaca atgccaattt
    33181 cagggcaaac tcccttaaaa ttagatttac ggatagccga cttaagttta gagtgacaac
    33241 acttcgtgtg actaagggga caattcctgc ggactggagt ccatctccag atgacctaaa
    33301 agcctactcg gatactaagc ttgaacagac agctaatgag attaaagcta gtgtaacaag
    33361 tcttgatcat aaaactctta agcaaactga tattactatg acttctgaag ggattgtcct
    33421 aagagcaggc aagacgagta atgatgtggc aagagctatt ggctcttact ttaaggtcac
    33481 tcctgatgct atagctctgt ttagctcact gataaaagtt agcgggaaca tgttggttga
    33541 tggttctgta actagtcgta atttggtaac aggcgctgtc gaaacgggac atgttaaggc
    33601 tggagcaatc acaggagtgc ttttagcagc tgaagctgta acggcagaaa aacttaaagt
    33661 cgaccaagct ttctttaaca agttgatggc caacgatgcc tacttgaagc aactatttgc
    33721 caagtcagcc ttcatcactc aggttcaatc cgtgacaata tctgccagtc agatttcggg
    33781 cggtgttatt aaagctttga ataatgccat ggaaatccag atgaacagtg gtcagatact
    33841 ttactacact gaccaagctg cactgaaacg ggttttgagt ggctatccta ctcagtttgt
    33901 taagtttgca actggtacag tatctggtaa aggaaatgct ggtgttacgg tcattggttc
    33961 taaccgctac ggtacagaat caaccaatga tggtggattt gttggagtcc gtgcatggaa
    34021 cggctcaaac attgactcac ttgacttagt tggtgataaa atccgattag caagctctgc
    34081 ctttgacaat tcagatggtt gggatgttcg gacacttgat tcaggcttga aaatcacacc
    34141 acacaatagg gctgctgaac gcaatagccg aattgaggtt ggtgatgtct ggattttaaa
    34201 aggaaatgga tcctattctt ctctcagaga tattttgaac tcattcaatg agaacttctc
    34261 aaaaggccca aatgctgact cttacactta ctaccattcg gggttctaac tagaaaggaa
    34321 caatcatgaa ccaagaacaa caactcaatc aagcgctacg cttgaccgtc aatgaattga
    34381 cagcccagtt ggccaatgag tcaaccacta agaacttgct ggctatccag ctgactgaag
    34441 ttgatcagga gaaacagcaa ctgactcagc aaaatgctga acttcaagca agagtttccg
    34501 aactagaagg cttgcttgat gaacaaactc aaccagaaat cattgaagga gaataaccat
    34561 gactacaaca gaaaataatt tacttgacct tgaaacaatc acagaaccct ttgacttggc
    34621 gacagcactc aagtacatga aggaaaatgg tgagttaatc cgttgcaaga atgctgtcaa
    34681 tgacttctac atgtaccgtg acatgcagaa acgtcctgtc atcgtcaatg gtcgccgtca
    34741 attcaaggac gtggaaactg tctgggcatt taaccaatgg ggcgggacaa cacccacaat
    34801 caacatggct gatctcttta atcttgaata ctacatcatg acctttgatg agaacggaaa
    34861 tcctgactgg actgagcctc acttggagga taagtaagct catctaaaat aaacaggata
    34921 ggagatagtg aatgccacca tggttaatag atagcactgt ggttgtggcg atggtgacgg
    34981 tgctgggcgg tctgttcagc actattatca ccacttcagc caatcggaaa gaccagctga
    35041 taaaacatca gtatgaggat atcaaggaag atttgtctgg cttgattgac aaggttaaga
    35101 cgattgatca tacaaccacg gaaacaaaga aaatttcaga aatcacaaaa gacggtacgc
    35161 ttaaaatcca gcgttaccgt ctttttcacg atttgacaaa ggaaatttct caaggctaca
    35221 caactattga acatttcagg gagttgagca ttctctttga gagttatcaa ctgcttggcg
    35281 gaaatgggga aattgaagcc ttgtttgaga aattcaaaca gttaccaatt gaggaggact
    35341 aaacatgaca caaattacag atattatcat cagctcagct atgggaattt tgactatttt
    35401 ggcaggaatt gcagtgcaag ctgtcaaggt ttatttgatt aaaaaaggcg gtgaaaaggc
    35461 cgtcttgatt acagaaatct tggccaagaa cgctgttaat gctgtggagc aagtggccac
    35521 tgaaactggc ttcaaaggag ctgacaagct aacttctgct aaggctcaga ttttagctga
    35581 gttgcaaaaa tacaatatcc acatgtcaga cgatgatctc actttgtttg ttgaatcatc
    35641 tgtcaaacaa atgcacgatg cctggaagga gtaacctatg gaaatcaaca cagaaacagc
    35701 cattgcctgg atgtctgcac gtcaaggcaa ggtcagctat tccatggact accgtgacgg
    35761 acctaacagc tatgactgtt ccagctctgt ctattatgct ctcaggtcag ctggtgcaag
    35821 ctcagcaggt tgggcggtca acactgagta catgcacgat tggctgatta aaaacggtta
    35881 tgagcttatt gctgaaaacg tggattggaa tgctgtccgt ggtgatatag caatttgggg
    35941 catgcgaggg cactcaagcg gagctggtgg tcatgtagtc atgtttattg accctgaaaa
    36001 tatcattcac tgtaactggg caaataatgg catcacagtg aacaactaca atcagacagc
    36061 ggctgctagt ggctggatgt attgctacgt ttaccgtttg aaaagcggag cttctaccca
    36121 aggaaaaagc cttgatacct tggtcaagga aacccttgct ggtaactacg gtaatggcga
    36181 agcacgcaag gcagtgcttg gcaatcaata tgaggctgtt atggcagtca tcaatggcaa
    36241 aactacgact aatcaaaaga ctgttgacca acttgctcaa gaggtaatcg ctggcaaaca
    36301 tggcaacggt gaagctcgta aaaagtcgct tggtagtcaa tatgatgcag ttcagaaacg
    36361 agtgacggaa ttgctcaaaa aacagccctc tgagccgtct aaggctcaag aggtaaacaa
    36421 gcccacggaa accaaaacaa gccaaacaga gctaactgga caagccacag ccaccaaaga
    36481 agagggcgac ctctctttca atgggactat cttgaaaaaa gcggtgctgg acaagattct
    36541 gggcaactgt aaaaagcatg atattcttcc aagttatgca ctgactatcc tacactatga
    36601 aggtctttgg ggaacttcag cagttgggaa ggcagataat aactggggcg gtatgacctg
    36661 gactggtcaa ggcaaccgtc caagcggtgt cactgtcaca caaggttccg cacgtccttc
    36721 aaatgagggc ggtcactata tgcactatgc aagcgtggat gactttctga cagactggtt
    36781 ttatcttcta agggctggtg gctcttacaa ggtgtctggt gctaaaacct tcagtgaggc
    36841 tatcaaggga atgtttaaag ttggcggtgc tgtctatgat tatgctgcaa gcggctttga
    36901 tagctatatt gtcggagctt caagccgcct caaggctatt gaggcagaaa atggttcact
    36961 ggacaagttt gataaaacta ccgacattgg tgacggtagc aaagacaaga ttgacattac
    37021 tattgaaggt attgaagtta ccatcaacgg tatcacttat gaactgacta aaaagccagt
    37081 ttagaattag atacgataag acaaaataag ccctcagcaa ttgccggggg ctgtttttcc
    37141 gttataacgg actttcctca aaatatcggt tataatggac tttttttgat aaaaatttta
    37201 aaaatatttg ttaaaaacaa gtgttttcta ttgacaagtg tttaaaacaa gtgtataata
    37261 caattaaaga taaggaaagg agataagcca atgacagagc gagagcttaa gaaaattgct
    37321 aagaagcaag gttttagtaa aactaacttt ggcaaagggt cacacgaagt ttggaaacat
    37381 ccagacggac ggacagtgac gatacctaag ccaaaggaaa aagattacag accaggcaca
    37441 ctgaacaaca ttctcaaagt cttgtatggg gagtgaggac gctcctcccc gtacccctaa
    37501 aaggggatac tctgatcatt ggcttaatct aatattatga aatacactta tttagcattg
    37561 tttgaagtgg ataaagaaaa cggtggctat aacatttctt tccctgattt tcctggagca
    37621 tttagtgaag ctgatagctt gaacgaggcc atttttaacg ctcgtgaagt ccttgaaatc
    37681 tatacaatta tgtttgaaga cgagggcaaa gagtttccta aagcatcatc attcaaggct
    37741 cttgcaagca atttagcaag tgacgaagat gtgattcagg ctatttcagt tgatactgag
    37801 cttgtccgtg agcgtgaacg ctctaagatt gtcaacaaga ctgtcacact gccaagctgg
    37861 cttgtggaag ttggaaaaga aaacaaggtc aactttagcc agctgttgca aaaagcaatc
    37921 cgtgaggaat tgcaagtata agaaagagcg gtcaagatgg ccgctttttt ctgtataaaa
    37981 aattatgaaa atagatagta atacttttca aaaattgacc tatgaaaaac cgtccgcccc
    38041 aaattcgccc caaattattt ttaaagttag cctgatttaa cctgatggaa aatcaaaaaa
    38101 gcctataaaa tcaggctttt cattcggtta attcctgtta attcaggtac tgaaaggcgg
    38161 tagacggatt tgaaccgacg atcaagcttt tgcagagccg tgccttacca cttggctata
    38221 ccgccataac agataatatt ctatcgaaaa aaaaaagatt cgtcaagtct ctttcgtaaa
    38281 atagccaatt ttagccctta aacttcctca tattaccaaa aaaagagatt gatacaatgg
    38341 gtatcaatct cttttagatt attgtctgat taaataatca aatgcgccaa tggcagctgt
    38401 ggcaccagac cccatggaaa taatgatttg cttgtaggca gcatctgtac agtcacctgc
    38461 agcaaaaata ccagggatat ttgttgagcc aaatttatca acgacaattt cttgacgttc
    38521 attgagttca atgccgctat ccttgagcca agaagtactt ggtacaagac caatttgtac
    38581 aaaaacacct tctaaatcaa tgtgtttttc ttcgtttgta tcacgatcgg tatagtttaa
    38641 accagtgaca tgatcttcac caacgatatc tttagtagca acattcttaa gaatagttaa
    38701 attgtcagtt ttagctgctc gttcttgcag tacttggtct gctttgagct caggtaagaa
    38761 ttctaggact gtaacatgtt tagtgacacc ggctagatct aaagcagctt ccatacctga
    38821 attaccacca cctataacag caacattttt tccttcgaaa agagggccat cacaatgtgg
    38881 acagtaagta acacctttat tgcggaattc ttcctcgcca ggaacgttga tatttcgcca
    38941 cttagcaccc agagctagga tagcagtttt agcttgtaaa atagctccgt tagccagtgt
    39001 tacctcaact aattcttttt tctcaatgcc agttgctaat tgactcttga taatatcaat
    39061 atcgtaagat ttggtatgct cttcaatttg agccattaat ttagggcctt cggtgtacag
    39121 tgtaccaatc atgttctcaa taccaactgt ttcgataact tgtccaccaa aagtttctgc
    39181 taaaatacct gtttttaacc ctttacgagc ggcataaatt gcagcactgt tacctgcagg
    39241 accaccacca ataactagaa catcatagac acctttgtca gcaaatgctt cagcatcaag
    39301 aggtccatct agttgttcta aaagctgttc aattgtagca cggccagaag taaattcttc
    39361 ttggtcctta taaacagtag gaacagacat gatacctttt gatttgactt cgtcttgata
    39421 catgcctcct tcaatcatag tatgagtgat atttggattc aaaacagcca ttatattaaa
    39481 ggcttgaacg acatctggac aattatggca agttaatgaa acataagttt ctagattgat
    39541 agttttctca atccccttaa ttcgtttgat aatatcttca tcaacttttg gtgcgcgacc
    39601 agagacttgc aacaaagcta aaatgaaaga tgtgaattca tgccccattg gcaagcctga
    39661 aaaaataacg cgactttctt gccctttttt tgcaatccca aaactaggtt gacgttttaa
    39721 atgtgttgat tcaaggctga tacgatcaga catagcgaca atttcatcta gaaaatcctt
    39781 tactttttga gaattatcgt tatctcctaa atcagtttgt aatacaatat cagattctag
    39841 caaatctaaa tattgcgcta attgagcctt gatttcttta tctaagacca tggattacct
    39901 cctggttatt aaattttacc tacaaggtct aaactaggag ttaaagtttc agctccttct
    39961 ttccatttag ctggacaaac ttcacctgga tgttggcgga tgtattgagc agcacggact
    40021 ttatcaatga gagtacttgc atcacgtccg atgccatcag cattaatttc catcatttgg
    40081 atgacaccgt ctggatcaat gataaatgta ccacgttgtg ctagaccatc ttgtccaagg
    40141 acatcaaaac cttgtgaaat aaggtgtgaa gggtcaccaa tcataggata tgtgatagta
    40201 ccaacgacat ctgagtcatc atgccatgct ttatgaacaa aatgagtatc agtagagaca
    40261 gaataaactt ctacatcaag agatttaagt gtttcgtatt gctcttgaag gtcaccaagt
    40321 tcagttgggc aaacaaatga aaagtctgct gggtagaaac aaaaaactgc ccattttcct
    40381 ttaacgtctt catttgtaac agtgatgaat tttccatcgt gataagcttg agctgaaaat
    40441 tcaataattt cttttccgac taatgacata ataatgtcct cctttatttt ttaatagcct
    40501 aattataaac gaaatacttt taaatctcca ataaaaacgt ttacattttt ctaagaaaag
    40561 tatgattttt ctaatagagt ggaagatttt atagcttgtc aaatctaact ttttttgata
    40621 aactactata gttgcttaaa gtgacaaata ctcatcttag atcaattgtg gtcttgttgc
    40681 cgaaaggtta ggctgcaagt cgaagtctga gagaggaaaa aacattttta aaggagaact
    40741 tactcatggc agtaatttca atgaaacaac ttcttgaggc tggtgttcac tttggtcacc
    40801 aaactcgtcg ctggaaccct aagatggcta agtacatctt cacagaacgt aatggtatcc
    40861 acgttatcga ccttcaacaa actgtaaaat tagctgacca agcttacgaa tttgttcgtg
    40921 atgctgcagc aaacgatgca gttatcttgt ttgttggtac taagaaacaa gcagctgagg
    40981 ctgttgctga agaagctaaa cgtgctggtc aatacttcat taaccaccgt tggttaggtg
    41041 gaactcttac taactgggga actatccaaa aacgtattgc tcgtttgaaa gaaatcaaac
    41101 gtatggaaga agaaggaact tttgaacttc ttcctaagaa agaagttgca ttgttaaaca
    41161 aacaacgtgc acgtcttgaa aaattcttgg gtggtatcga agatatgcca cgcatcccag
    41221 atgtaatgta tgttgttgac ccacataaag agcaaatcgc tgttaaagaa gctaaaaaac
    41281 ttggtattcc agttgtagct atggttgaca ctaacgctga tccagatgat atcgatgtta
    41341 tcatcccagc aaacgatgac gctattcgcg ccgttaaatt aattacttct aaattagctg
    41401 atgcagttat tgaaggtcgt caaggtgaag atgctgacgt tgacttcgct caagaagcac
    41461 aagcagattc aattgaagaa atcgttgaag tagttgaagg atcaaacaac gactaatatt
    41521 taatttattg taaatcctaa aacggggcag atgcaaaccg ctctgtctcg tttttttaat
    41581 cattatttga aattaagagg agaacgaaaa tggcagaaat cacagctaaa cttgtaaaag
    41641 aattgcgtga aaaatcaggt gcaggcgtta tggacgctaa aaaagcatta gtagaaactg
    41701 atggtgacct tgataaagcg attgaattac ttcgcgaaaa aggtatggct aaagcagcta
    41761 aaaaagcaga ccgtgttgct gctgaaggtt taacaggtgt ttatgttgat ggtaacgttg
    41821 cagcagttat tgaagttaac gctgagactg actttgttgc taaaaacgac caatttgtaa
    41881 cattagttaa tgaaactgct aaagttattg cagaaggacg tccttcaaac aatgaagaag
    41941 cacttgcttt aacaatgcct tcaggtgaaa ctttagaaca agcatttgta actgcaactg
    42001 ctacaattgg tgaaaaaatt tcattccgtc gcttcgcttt agttgaaaaa actgatgagc
    42061 aacattttgg tgcataccaa cataatggtg gacgtatcgg agtaattact gttgttgaag
    42121 gtggcgacga tgcacttgct aaacaagtat caatgcacgt tgcagctatg aagccaactg
    42181 ttctttcata tactgaactt gatgcacaat ttgttcatga tgaattagca cagttgaacc
    42241 acaaaattga gcaagacaac gagagtcgtg ctatggttaa caaaccagct cttccattcc
    42301 ttaaatatgg atcaaaagct caattgactg atgaagttat tgcccaagca gaagaagaca
    42361 tcaaagcaga attagctgca gaaggtaaac cagaaaaaat ctgggataaa atcgttccag
    42421 gtaaaatgga tcgtttcatg cttgacaaca ctaaagttga ccaagagtac acactccttg
    42481 ctcaagttta catcatggat gatagcaaaa cagttgaagc ttatcttgag tcagtaaatg
    42541 ctaaagcagt tgctttcgta cgttttgaag ttggagaagg tattgagaaa gcttctaacg
    42601 actttgaagc agaagttgca gctacaatgg cagcagcact tgaaaaataa ttaacactcg
    42661 ctttatatta taatttggca gcaaaatgtt gtcagtaaat atattttaaa ataagacact
    42721 tggcttaagt caagtgtctt attttgctat cgaattggtg gattggcaaa tattaaaaag
    42781 gagagacagt catctcacct taacttttct agaataggtt gaagccgata atggataatg
    42841 ttcccatgat gcatccagct agtataacac ctagcataac agcaataaca ctatgtttaa
    42901 attcccaatc taggaggcta gctgcaagtg tgccagtcca tgctcctgtt ccgggtaagg
    42961 gaatgcctac aaacaatagc aaggcaataa acaatccttt ttctccagct actttttcaa
    43021 gtttttgacc accgctatgt ccttttttaa ggcaccatgt gaaaaactta ccagtataag
    43081 gtttatctgc tccccattct agaactttgc gagcaaagaa gaagatgata ggtacaggta
    43141 gcatgtttcc aacaacaccg atagctagcg cttcccacaa aggtattcca ttagcgattg
    43201 caaatggcac agctcctcgt agttccacta agggaatcat tgaaatcaaa aatgtaataa
    43261 tgtaattcat taaataaaac ctttctatgg tattattcca ttttatctta tcttgctagt
    43321 aatgacaact aaaatccgcg gtcattctag tttta
//
LOCUS       DATROQ010000007        42448 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.007, whole genome shotgun sequence.
ACCESSION   DATROQ010000007 DATROQ010000000
VERSION     DATROQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42448
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.007"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            893..2017
                     /gene="mutY"
                     /locus_tag="VBC94_000335"
     CDS             893..2017
                     /gene="mutY"
                     /locus_tag="VBC94_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEO6978637.1"
                     /translation="MWPEDRIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YSKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFQREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            2194..2787
                     /locus_tag="VBC94_000336"
     CDS             2194..2787
                     /locus_tag="VBC94_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6978638.1"
                     /translation="MEIGQQIIRYRKQQALSQEELAEKVYVSRQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFERDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQTYKEILAVSSGKLLDEIEKREE
                     RAKLPYQKPLIVTVFFLITVATFFASRFIFTWLFHLS"
     gene            complement(2833..3147)
                     /gene="trxA"
                     /locus_tag="VBC94_000337"
     CDS             complement(2833..3147)
                     /gene="trxA"
                     /locus_tag="VBC94_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEO6978639.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(3228..3728)
                     /locus_tag="VBC94_000338"
     CDS             complement(3228..3728)
                     /locus_tag="VBC94_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6978640.1"
                     /translation="MESYEQFYAKLSQPFRKSPQLIILLNFLLKIVTGMMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRLDFPRPYEKWNIKPLIYKDTEGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLIL
                     GLCLFI"
     gene            complement(3732..6071)
                     /locus_tag="VBC94_000339"
     CDS             complement(3732..6071)
                     /locus_tag="VBC94_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEO6978641.1"
                     /translation="MNNKILEQLEFNKVKELILPYLKTEQSQEELSELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEESRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPEISNDSTLALINVRHPLLSNPVANDLHFDQDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADHNSLVLFDELGAGTDPQEGASLAMAILEHLRLSNIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQ
                     KVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDNFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG"
     gene            complement(6156..6698)
                     /locus_tag="VBC94_000340"
     CDS             complement(6156..6698)
                     /locus_tag="VBC94_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEO6978642.1"
                     /translation="MLSLLLLIIVIWHFYIGYSRGIFLQVFYVLMSMVSLMIASQFYQ
                     ELASQITLWVPYSNPVQGVEVYFFKDISKFQLSHVYYAGVAFVFIYSLSYLVGRLLGV
                     LLHLAPVEHFDSLQNNIISGFLAVLVCLLFMSMCLTILATVPMSFVQEKLWNSLFVRF
                     LINDLPFFSQFLVRTWAVGL"
     gene            complement(6701..7012)
                     /locus_tag="VBC94_000341"
     CDS             complement(6701..7012)
                     /locus_tag="VBC94_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978643.1"
                     /translation="METKINNSQKNRYKFVFGDKPLTLTTDKDNLFMEEIERVATEKY
                     EAIKEKLPNADNETIAILMAINALSVQLSREIDIEKMEDELNKLRSKTISDIKEKVSE
                     D"
     gene            7123..8016
                     /locus_tag="VBC94_000342"
     CDS             7123..8016
                     /locus_tag="VBC94_000342"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEO6978644.1"
                     /translation="MNTIVMQADKKLQEKIRTDLAQHHISNNNPYVVFSAKISGATVL
                     LYTSGKLVFQGSNASHIAQKYGFIEQKESCSSESQDIPIIGTDEVGNGSYFGGLAVVA
                     SFVTPKDHAYLKKLGVGDSKTLTDQKIKQIAPLLEKAIPHKALLLSPQKYNQVVSPNN
                     KHNAVSVKVALHNQAIFLLLQDGFEPEKIVIDAFTSSKNYQNYLKNEKNQFKQTITLE
                     EKAENKYLAVAVSSIIARNLFLENLNKLSDDVGYKLPSGAGHQSDKVASQLLKAYGIS
                     SLEHCAKLHFANTKKAQALLK"
     gene            8032..8625
                     /gene="lepB"
                     /locus_tag="VBC94_000343"
     CDS             8032..8625
                     /gene="lepB"
                     /locus_tag="VBC94_000343"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6978645.1"
                     /translation="MKEFIKEWGVFILILSLFLLSRIFLWQFVKVDGHSMDPTLADKE
                     QLVVLKQTKINRFDIVVANEEEGGQKKKIVKRVIGMPGDVIKYKNDTLTINNKKTEEP
                     YLKEYTKLFKKDKLQEKYSYNPLFQDLAQSSTAFTTDSNGSSEFTTVVPKGHYYLVGD
                     DRIVSKDSRAVGSFKKSTIVGEVKFRFWPIRRFGTIN"
     gene            8754..11174
                     /locus_tag="VBC94_000344"
     CDS             8754..11174
                     /locus_tag="VBC94_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEO6978646.1"
                     /translation="MEVFFTGTIERIIFENASNFFKILLLEIEDTDSDFDDVEVIITG
                     TMADVIEGEEYTFWGTLTQHPKYGEQLQSVRYERAKPTSGGLVKYFSSEQFKGIGKKT
                     AQRIVELYGDNTIDKILESPEQLSTISGLSKINREAFIAKLKLNYGTEQVLAKLAEYG
                     LSNRAAIQIFDHYKEESLEVINENPYQLVEDIQGIGFKIADQLAEQVGIESDSPKRFR
                     AAIIHTLVESSMEQGDTYIEARTLLEKTITLLEEARQIELDPSIVAKELTNLIAEDKV
                     QHIGTKIFSNTLFFAEEGIKKNLQRILNQPLDKQLNHKDIDREIRDIQKSLNIHYDNI
                     QEKAIREALLSKVFILTGGPGTGKTTVINGIIEAYSELHHIDLNKNDIPIVLAAPTGR
                     AARRMNELSGLPSATIHRHLGLNGDSDYQSLDDYLDCSLIIIDEFSMVDTWLANQLFD
                     ALDSHTQVIIVGDSDQLPSVGPGQVLADLLNINALPHVKLEKIFRQSEESTIVTLANQ
                     MRQGFLPEDFTAKKADRSYFEASANIIPNMISKIVQSALKSGIEAHEIQILAPMYRGQ
                     AGINNLNLIMQNLLNPLKDNNQFTFNDINFRIGDKVLHLVNDTELNVFNGDIGYITDL
                     IPAKYTESKQDEIYMTFDGQEVIYQRKEWLKITLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRMLQRNLIYTAITRSKSKLILLGEIGAFDFAVKNEGAKRNTYLIERFENKQEIANS
                     QKIEDSSIDQKIDNTIINTSIPKTATPIEQTNLSKITYRLTEENYLTIDPMIGINQQD
                     ISAIFDSK"
     gene            11288..11770
                     /locus_tag="VBC94_000345"
     CDS             11288..11770
                     /locus_tag="VBC94_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978647.1"
                     /translation="MVSYEKVRRSLRTATITIIVLNSLSLVFRLFTGISVQLAKTEIN
                     KGNTGNLPKEHIEAVLSATTPFMLFVTALIVLVNIAIVIFCIKNLRAIKRNQMVNYLP
                     YYLGFAITVGLVILGFLTTKAPWAIAINIVFQAIFGLLYFHAYQKAQKLNERDLEETN
                     "
     gene            complement(11841..12950)
                     /gene="dinB"
                     /locus_tag="VBC94_000346"
     CDS             complement(11841..12950)
                     /gene="dinB"
                     /locus_tag="VBC94_000346"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEO6978648.1"
                     /translation="MGERTMLIFPLINDTSRKIIHIDMDAFFASVEERDNPSLKGKPV
                     IIGSDPRKTGGRGVVSTCNYEARKFGVHSAMSSKEAYERCPQAIFISGNYQKYRQVGM
                     EVRDIFKKYTDLVEPMSIDEAYLDVTENKMGIKSAVKLAKMIQYDIWNDVHLTCSAGI
                     SYNKFLAKLASDFEKPKGLTFILPDQAQDFLKPLPIEKFHGVGKRSVEKLHALGVYTG
                     EDLLSLSEISLIDMFGRFGYDLYRKARGINASPVKPNRVRKSIGSEKTYGKLLYNEAD
                     IKAEISKNVQRVVASLEKNKKVGKTIVLKVRYADFETLTKRMTLEEYTQDFQIIDQVA
                     KAIFDTLEESVFGIRLLGVTVTTLENEHEAIYLDF"
     gene            13120..15432
                     /gene="pflB"
                     /locus_tag="VBC94_000347"
     CDS             13120..15432
                     /gene="pflB"
                     /locus_tag="VBC94_000347"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEO6978649.1"
                     /translation="MATVKTNTDIFEQAWEGFKGVDWKEKASIARFVQANYAPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEETRFPMDTRVASISELPAGFIDKDNELIFGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYATTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLAVYGADYLMQEKVNDWNALNDIDEESIRLREEI
                     NLQYQALGEVVKLGDLYGVDVRKPAMNTKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFARTKAYDALYSGDPTFITTSMA
                     GMGADGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWSDQLPYAFRRYCMSMSHKHSSI
                     QYEGVSTMAKEGYGEMSCISCCVSPLDPENEDKRHNLQYFGARVNVMKALLTGLNGGY
                     DDVHKDYKVFDIDPIRDEVLNFDTVKANFEKSLDWLTDTYVDAMNIIHYMTDKYNYEA
                     VQMAFLPSHVRANMGFGICGFANTVDSLSAIKYATVKPIRDEDGYIYDYETVGDFPRY
                     GEDDDRVDSIAEWLLEAFHGRLAKHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKG
                     VYLNEDGSVNLSKVEFFSPGANPSNKAKGGWLQNLNSLSKLDFAHANDGISLTTQVSP
                     RALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLKDVYDKIMNGEDVIVRISGYCV
                     NTKYLTPEQKTELTQRVFHEVLSMDDALTN"
     gene            15533..15906
                     /locus_tag="VBC94_000348"
                     /pseudo
     CDS             15533..15906
                     /locus_tag="VBC94_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
     gene            complement(15941..16870)
                     /locus_tag="VBC94_000349"
     CDS             complement(15941..16870)
                     /locus_tag="VBC94_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEO6978650.1"
                     /translation="MIKTNTVLELIKEQIKDNLYHGASLAIYENGEWHEHYLGTIDGN
                     EKVKAGLVYDLASVSKVVGVGTLLAKLVYQGTIDIDKPLRYYYPTFHHQTLTVRQLAT
                     HSSGIDPFIPNRDQLNATQLKDAINHIKVLEDKSFKYTDINFLLLGFMLEEVLGDSLD
                     KLFKRYIFTPFQMKETSFGPRVEAVPTVAGINDGIVHDPKAKVLGKHTGSAGLFSTID
                     DLQRFSIHYLKDDFAKPLWNNYSLSKSRSLAWDIDKDWINHTGYTGPFIALNYQKQAA
                     AIFLTNRTFSYDDRPLWIKKRRHVQEAITSYFE"
     gene            complement(16867..17622)
                     /locus_tag="VBC94_000350"
     CDS             complement(16867..17622)
                     /locus_tag="VBC94_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEO6978651.1"
                     /translation="MTLFHSLTFKHPVLRVNNRDLNIAFYQESLGFKLISEENAIAVF
                     SAWQNKEASFIIEESPTYRTRAVNGTKKLAKIIVKSQDAKDIEKLLANGAQAIQVYQG
                     QNGYAYETVSPEGDLFLLHAEDDLSQLVAIERPELEKKDDTTGLSNFAFQSISLNVPD
                     AVKAEAFYDKVFAGKFPINLSFKEAQGQDLQIAPNETWDIEILECCVNEDTNLNDLKS
                     TFESWGLDVYLDSKEKILVISDTSNIEIWISKE"
     gene            17747..18643
                     /locus_tag="VBC94_000351"
     CDS             17747..18643
                     /locus_tag="VBC94_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEO6978652.1"
                     /translation="MNETLLLHGIQLILIIAMIITFYQIVRHIRSQKINPFKRFFTGL
                     WIGFVTDALDTLGIGSFATTTTFFKLTKLVEDDRKIPATMTAAHVLPVLLQSLCFIFV
                     VKVEALTLITMAGAAFIGAFVGAKMTKNWHAPTVQRILGTLLITAAIIMLYRMITNPG
                     AGISDSVHGLHGIWLFVGIVFNFIIGVLMTMGLGNYAPELIFFSLMGLSPAVAMPVMM
                     LDAAMIMTASSTQFIKSGRVNWNGFAGLVTGGILGVIVAVLFLTNLDLNSLKTLVVGI
                     VLFTGAMLIRSSFKKTMPKSHH"
     gene            complement(18722..19570)
                     /locus_tag="VBC94_000352"
     CDS             complement(18722..19570)
                     /locus_tag="VBC94_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6978653.1"
                     /translation="MIEITWTVKYITEFIATAFLIILGNGAVANVDLKGTKGNNSGWI
                     IIAIGYGLGVMMPALMFGNVSGNHINPAFTLGLAFSGLFPWAHVGQYILAQILGAIFG
                     QLVVVMVYQPYFVKTENPNHVLGSFSTISALDDGQKSSRKAAYINGFLNEFVGSFVLF
                     FGALALTKNYFGVELVGKLVQAGYDQTTAATRISPYVTGSLAVAHLGIGFLVMTLVAS
                     LGGPTGPALNPARDLGPRIVHRLLPKQILGQAKEDSKWWYAWVPVLAPIVASILAVAL
                     FKLLYL"
     gene            complement(19738..20190)
                     /locus_tag="VBC94_000353"
     CDS             complement(19738..20190)
                     /locus_tag="VBC94_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO6978654.1"
                     /translation="MTQKYERILIAIDGSYESELAVEKGINVALRNDAELLLTHVIDA
                     HAYQSEGVFSDYVFDRQEQESADVLAYFEKLAHSKGLTKIKKITEIGNPKTLLAKDIP
                     IREKADLIMVGATGLNTFESLLIGSTSEYILRHSKVDMLVVRDSKKTL"
     gene            complement(20208..21410)
                     /locus_tag="VBC94_000354"
     CDS             complement(20208..21410)
                     /locus_tag="VBC94_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6978655.1"
                     /translation="MEDKLFNKHFIGITILNFIVYMVYYLFTVIIAFIATKELGVSTS
                     QAGLATGIYIVGTLIARLIFGKQLEVLGRKLVLRGGAIFYLLTTLAYFYMPSIGVMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPADKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HINFKMVIVLCSILIAIVVLGAFVFPVKNITLNPEQLAKSKSWTIDSFIEKKAIFITI
                     IAFLMGISYASVLGFQKLYTTEINLMTVGAYFFIVYALVITLTRPSMGRLMDAKGDKW
                     VLYPSYLFLTLGLALLGSAMGSVTYLLSGALIGFGYGTFMSCGQAASIKGVEEHRFNT
                     AMSTYMIGLDLGLGAGPYILGLVKDGFLGAGVQSFRELFWIAAIIPVVCGILYFLKSS
                     RQVETKTI"
     gene            21566..22225
                     /locus_tag="VBC94_000355"
     CDS             21566..22225
                     /locus_tag="VBC94_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO6978656.1"
                     /translation="MNHQILQNYINSHNLPIIEKDYHKYLTFESLEEDYTYILKDGIV
                     KQSVLSKYGTEFNLRYVTGLEITSILNTDYSQHMGEPYNVRIESETAHFYKVRRSTFL
                     KDINNDIELQGYVKDFYHNRLEKSMKKMQCMLTNGRIGAISTQLYDLSKMFGEERDNG
                     DIYINFVITNEELGKFCGISTGSSVSRILKQLKDDHIIRIEKQHIIITNVEKLKDHIV
                     F"
     gene            22249..24534
                     /locus_tag="VBC94_000356"
     CDS             22249..24534
                     /locus_tag="VBC94_000356"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEO6978657.1"
                     /translation="MRYNQFSYIPTKPNEAFEELKGLGFPLNKKNSDKANLEAFLRHS
                     FLNQTDTDYALSLLIVDAKTDALTFFKSNSDLTLENLQWIYLQLLGFIPFVDFKDPKA
                     FLQDINFPVSYDNIFQSLHHLLACRGKSGNTLIDQLVADGLLHADNHYHFFNGKSLAT
                     FNTNQLIREVVYVETSLDTMSSGEHDLVKVNIIRPTTEHTIPTMMTASPYHQGINDPA
                     ADQKTYQMEGALAVKQPKHIQVDTKPFKEEVKHPSKLPISPATESFTHIDSYSLNDYF
                     LSRGFANIYVSGVGTAGSTGFMTSGDYQQIQSFKAVIDWLNGKVTAFTSHKRDKQVKA
                     NWSNGLVATTGKSYLGTMSTGLATTGVEGLKVIIAEAAISTWYDYYRENGLVCSPGGY
                     PGEDLDVLTELTYSRNLLAGDYIKNNDCYQALLNEQSKAIDRQSGDYNQYWHDRNYLT
                     HVNNVKSRVVYTHGLQDWNVKPRHVYKVFNALPQTIKKHLFLHQGQHVYMHNWQSIDF
                     RESMNALLSQELLGIDNHFQLEEVIWQDNTTEQTWQVLDAFGGNHQEQIGLGDSKKLI
                     DNHYDKEAFDTYCKDFNVFKNDLFKGNNKTNQITINLPLKKNYLLNGQCKLHLRVKTS
                     DKKAILSAQILDYGPKKRFKDTPTITFLNSLDNGKNFAREALRELPFTKDHYRVISKG
                     VLNLQNRTDLLTIEAIEPEQWFDIEFSLQPSIYQLSKGDNLRIILYTTDFEHTIRDNA
                     SYSITVDLSQSYLTIPTNQGN"
     gene            24538..24897
                     /locus_tag="VBC94_000357"
     CDS             24538..24897
                     /locus_tag="VBC94_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978658.1"
                     /translation="MKLLTKERFDDSQHFWYQINLLQESNFGAVFDHDNKNIPQVVAT
                     IVDDLQGSGSSNYFWYFGNTTDTSILMIAHLNRKFYIQVNLKNFDFALNLIAINNWKS
                     LLQTQLEALNDTLAIFQ"
     gene            24943..25923
                     /locus_tag="VBC94_000358"
     CDS             24943..25923
                     /locus_tag="VBC94_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO6978659.1"
                     /translation="MSSYWNNYPELKKNIDETNQLIQERIQVRNKDIEAALSQLTAAG
                     GKQLRPAFFYLFSQLGNKENQDTQQLKKIAASLEILHVATLIHDDVIDDSPLRRGNMT
                     IQSKFGKDIAVYTGDLLFTVFFDLILESMADTPFMRINAKSMRKILMGELDQMHLRYN
                     QQQGIHHYLRAISGKTAELFKLASKEGAYFGGAEKEVVRLAGHIGFNIGMTFQILDDI
                     LDYTADKKTFNKPVLEDLAQGIYSLPLLLAIEENPDIFKPILDKKTDMATEDMEKIAY
                     LVVSHRGVDKARHLARKFTEKAISDINKLPQSSAKKQLLQLTNYLLKRKI"
     gene            complement(26008..27756)
                     /gene="cydC"
                     /locus_tag="VBC94_000359"
     CDS             complement(26008..27756)
                     /gene="cydC"
                     /locus_tag="VBC94_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEO6978660.1"
                     /translation="MFKIPLFKELKTDQWIKPFFKQYKVSLVIALFLGFMTFFSASAL
                     MFNSGYLISKSASLPSNILLVYVPIVLTRAFGIGRPVFRYIERLTSHNWVLRMTSQLR
                     LKLYHSLESNAIFMKRDFRLGDVMGLLAEDINYLQNLYLRTIFPTIIAWILYSFIIIA
                     TGFFSLWFALMMLLYLAIMIFLFPLWSILANGARQTREKELKNHLYTDLTDNVLGISD
                     WIFSQRGQEYVTLHERSESELMAVQKKIRSFDNRRALIVELVFGFLAILVIIWASNQF
                     IGHRGGEANWIAAFVLTVFPLSEAFAGLSAAAQETNKYSDSIHRLNELSETYFETTQN
                     QLPNKPYDFSVKNLSFQYKPQEKWVLHHLDLDIKEGEKIAILGRSGSGKSTLASLLRG
                     DLKASQGEITLGGADVSIVGDCISNYIGVIQQAPYLFNTTLLNNIRIGNQDASEEDVW
                     KVLERVGLKEMVTDLSDGLYTMVDEAGLRFSGGERHRIALARILLKDVPIVILDEPTV
                     GLDPITEQALLRVFMKELEGKTLVWITHHLKGIEHADRILFIENGQLELEGSPQELSQ
                     SSQRYRQLKAADDGDL"
     gene            complement(27749..29467)
                     /gene="cydD"
                     /locus_tag="VBC94_000360"
     CDS             complement(27749..29467)
                     /gene="cydD"
                     /locus_tag="VBC94_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEO6978661.1"
                     /translation="MLDKAVMRLSGIHKLLGLLAGLDVLQAIFIIGQAYYLSLSITGL
                     WEGQKLSSQTVYVLLFMVSYLGRHVIDYIKNRKLDDFSTAQSSLLRRQLLDKLFDLGP
                     KVVQEQGTGNVVTMALDGVSLVENYLRLVLNKMINMSIIPWIILAYIFYLDIESGAIL
                     LIVFPLIIIFMIILGYAAQAKADKQYESYQVLSNHFLDSLRGIDTLKYFGLSKRYGKS
                     IYQTSESFRKATMSTLKIGILSTFALDFFTTLSIAIVAVFLGLRLLNEQIYLLPALTI
                     LILSPEYFLPVRDFSSDYHATLDGKNAFQAIQKVLNKTGIKGEQLVIDDWSKESRLDL
                     ENIAIAYDQKRVVEDVTLRFRGHQKVALVGVSGSGKSSLINLLSGFLGPDNGSLKVDG
                     REVTNLDQEDWHKQMIYIPQTPYVFEMSLRDNITFYTPNASDEEVVRAIHMVGLDSLL
                     SELPDGLETRIGNGARPLSGGQAQRIALARAFLDQNRRIMVFDEPTAHLDIETELELK
                     EKMLPLMSDRLVIFATHRLHWLNQMDVIVVMEKGRVAEVGSYQELLAKKGYLYQLKHA
                     MGGNDV"
     gene            complement(29467..30486)
                     /gene="cydB"
                     /locus_tag="VBC94_000361"
     CDS             complement(29467..30486)
                     /gene="cydB"
                     /locus_tag="VBC94_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEO6978662.1"
                     /translation="MSALQFFWFFLIGLLFSGFFFLEGFDFGVGMAVQTLTHNEHEKD
                     QVVETIGPVWDGNEVWLLTGGGAMFASFPYWYASLFSGYYLILLTILFGLIIRGVSFE
                     FRHKVPAEKKQFWNWTLTIGSAIVPFFFGIMFISLIQGMPLDASGNLSAQFSDYFNIF
                     SLVGGVAMVLLAYLHGLNYIALKTEGPIRERARNYAQLLYWVLYLGLALFAVLLYFKT
                     DFFSNHPIVTTIMVLVIVVLAVLAHASTFKGAEMTAFLASGLSLVSVVVLLFQGLFPR
                     VMISSISPKYDLLIQNASSTPYTLKVMSIVAITLVPFVLAYTAWAYYIFRKRITLPVI
                     VTGEK"
     gene            complement(30487..31914)
                     /locus_tag="VBC94_000362"
     CDS             complement(30487..31914)
                     /locus_tag="VBC94_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEO6978663.1"
                     /translation="MTIETLARFQFAMTTVFHFFFVPFTIGTCLVVAIMETMYVITKN
                     EEYKKLTKFWGNIMLLSFAVGVVTGIIQEFQFGMNWSDYSRFVGDIFGAPLAIEALLA
                     FFMESTFLGLWMFTWDNKKISKKLHVTFIWLVVFGSLMSAMWILTANSFMQHPVGYEV
                     VNGRAQMTDFLALVKNPQFFYEFTHVIFGAITMGGTVVAGMSAFRLLKSEQLKDTTVE
                     LYKKSVRIGLVVALLGSISVMGVGDLQMKALIHDQPMKFAAMEGDYEDSGDPAAWSVV
                     AWANEAEHKQVFGIKIPYMLSILSYGKPSGSVKGMDTANKELVAKYGKDNYYPMVNLL
                     FYGFRTMAAMGTAIMGVSVLGLFLTRKKKPILYKHKWMLWIVALTTFAPFLANTFGWI
                     VTEQGRYPWTVYGLFKIKDSVSPNVSVASLFVSNTVYFLLFGGLAVMMISLTIRELKK
                     GPEYEDEHGHHGAYTSIDPFEKGAY"
     gene            complement(32017..33225)
                     /locus_tag="VBC94_000363"
     CDS             complement(32017..33225)
                     /locus_tag="VBC94_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6978664.1"
                     /translation="MKEILVLGAGYAGLKAVRNLQKQSGDFHITLVDMNDYHYEATEL
                     HEVAAGSQPKEKITFPIKDVINTNKVNFMQDEVLRVDAENKTVTVKNNGELHYDYVVV
                     ALGFVSETFGIKGAMENALQMTNISQAENIHNHIVNTMKLYRETKDENLLKLLVCGAG
                     FTGIELAGAMVDERPKYAALAGVKPEQIEIICVEAATRILPMFDDELAQYGVNLIKDL
                     GINLMLGSMIKEIKPGEVVYGTSKEDEELKSITAGTIIWTTGVSGSPVMGESGFDQRR
                     GRVMVNSDLRDPKYDNVYVIGDVSAFMDTESGRPFPTTAQIATRMGAHVAKNLLHQIK
                     GEATEDFSYSPQGTVASVGNTHGLGVVGKTKIKKYPASVMKKIIMNKSLVDMGGLKEL
                     LAKGRFDLYH"
     gene            complement(33238..34137)
                     /locus_tag="VBC94_000364"
     CDS             complement(33238..34137)
                     /locus_tag="VBC94_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEO6978665.1"
                     /translation="MTLPIFLELVEMKAKTASVLPFLIGLCFSAYYYNSVHPVYVGLF
                     FVAMFLFNMFVDIWNNYNDYRNAVDLDYKNDTNIIGRENLSLRQIEVIMASLVITSSM
                     IGLVLVSQVGLPLLWMGLFCFGIGVLYSFGPRPLSSLPLGEVFSGLTMGFMISLICVY
                     LNTYQNFSWDILNLSKIFLISLPNTLWIANLMLANNLCDKEEDEKNHRYTLVHYTGIR
                     GGLLLFAISNSIALLAIVFEFLFGLAPVTVLLSLLLIPFIYKQTKLLWQKQVKRETFV
                     CAVRILALGSATQVLTYLIGIIF"
     gene            complement(34546..35151)
                     /locus_tag="VBC94_000365"
     CDS             complement(34546..35151)
                     /locus_tag="VBC94_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978666.1"
                     /translation="MNKLITEKKVNNVSTANYLKLGLVSAMFAGGAFVALGSTQGVSA
                     STFTAPQATPPKAERQLTDSELYERAQKQVLPRYIQGSLSGILNQHSTLYKQQNAAVT
                     PQVSSPKSERQLTDSELYESAQKQVLPKYIQGSLSGILNQHSTLYKQQNAAVTPQVSS
                     PKSERQLTDSEIYERAQKQVLPNYIQGSLSGILNQHSTLNA"
     gene            36034..36333
                     /locus_tag="VBC94_000366"
     CDS             36034..36333
                     /locus_tag="VBC94_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEO6978667.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQTQADLAASQFTGKSAQELVTVTFT
                     GDKKLISIDYKEAVVDPEDIETLQDMTTQAINDALSQVDDATKKIMGAFAGKMPF"
     gene            36493..37683
                     /locus_tag="VBC94_000367"
     CDS             36493..37683
                     /locus_tag="VBC94_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6978668.1"
                     /translation="MVMDSLIIKQLIKSTFDIPLQVTYPNGNIETYNGSNPHVKLKLN
                     KNFSVSELSKDPSIVLGEAVMDGDIEIYGSIQELILSAYRCGDSFLRNSKFSKLIPKQ
                     FHDKKHSKSDIQKHYDIGNDFYKLWLDDTMTYSCAYFKHENDSLEQAQLNKVHHILNK
                     LNAQPGGKLLDIGCGWGTLIITAAKEYGLNATGITLSEEQASFITKRIKEEGLENKVT
                     VLIKDYRDIRETYDYITSVGMFEHVGKENLSQYFQTISKRLNINGLALIHGITGQVGG
                     NHGSGTNSWINKYIFPGGYIPRLTENLNHIASAGLQIADLEPLRRHYQKTLELWTKNF
                     HNALPEVQKTHDKRFINMWDLYLQSCAASFESGNIDIFQYLLSKGVSKDTMPMTRDYM
                     YSAN"
     gene            complement(37753..38478)
                     /locus_tag="VBC94_000368"
                     /pseudo
     CDS             complement(37753..38478)
                     /locus_tag="VBC94_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(38605..39192)
                     /locus_tag="VBC94_000369"
     CDS             complement(38605..39192)
                     /locus_tag="VBC94_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEO6978669.1"
                     /translation="MKQLQEYIAFDLEFNTVGEHSHIIQVSAVKYSNHQEIALFDTYV
                     HTKVPLQSFINGLTGITARDIIGAPKIEIVLTDFQSFVGDTPLIGYNGYKSDLPLLVE
                     NGLDLTSQYQVDLYDEAFVRRSTDLNGIVNLKLTTVADFLGIKGKAHNSLEDARMTAR
                     VYEKFLDLDENKIYLKQQKEVAVDSPFATLGNLFD"
     gene            complement(39236..39772)
                     /locus_tag="VBC94_000370"
     CDS             complement(39236..39772)
                     /locus_tag="VBC94_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEO6978670.1"
                     /translation="MTVEKTVSELAEILGVSRQAMNNRVKTLPEECVEKNSKGVTVVN
                     RDGLIKLEEIYKKTILEEEPIDEEASRRELLEILVDEKNTEITRLYEQLKAKDIQIAS
                     KDEQLHVKDIQIAEKDKQLDQQQQLTLTAMEDTQRLQLELNEAKAEVEEIQEAKEEKI
                     QELEAVKKSFFGRFFNKK"
     gene            39907..41079
                     /locus_tag="VBC94_000371"
     CDS             39907..41079
                     /locus_tag="VBC94_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6978671.1"
                     /translation="MKHYDTIIIGGGPAGMMAAISSNFYGNKTLLIEKNKRLGKKLAG
                     TGGGRCNVTNNGNLDELLAGIPGNGRFLYSVFSQFDNHDIINFFQDNGVTLKEEDHGR
                     MFPTTDKSRTIINALENKIKELGGQIMTDTEVVSVKKIGDSFYIKTKDTQFASDKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLEVTDMEAAESPLLTDFPHKKLQGISLDDVTLSF
                     EKHIITHDLLFTHFGLSGPAALRISSFVKGGETIYLDVLPNISVKELEIHFQNEREKS
                     LKNALKILLPERLAEFYAEDLPEKVKQVSVKDLEMLIQKLKKLPILVTGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVAGLHFAGEVLDINAHTGGFNITSALCTGWVAGSLHY"
     gene            complement(41123..41905)
                     /locus_tag="VBC94_000372"
     CDS             complement(41123..41905)
                     /locus_tag="VBC94_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HEO6978672.1"
                     /translation="MCYNNSMNERYIRFNPNIAKQKPSNKDKCPFCDKSQLGKILDVK
                     DDMIWVENKYPTLEETYQTLVIESNDHNGDISVYSESKMRQLLDYLLSKWQLMEESGH
                     YRSVVLYRNFGPLSGGSLRHPHSQIVGLKNVDAYETLNLDWFDGIEVCSETSQQAEIS
                     IANRPMLDFTEFTIKVSKLENAILIADSLPILCHYILNEFLGGRCKSYNLFFYKHDGG
                     LICKIVPRFIVSPYQIGYAIHQVNLPDELEKIKEALRAKYYL"
     gene            42064..42333
                     /gene="rpsN"
                     /locus_tag="VBC94_000373"
     CDS             42064..42333
                     /gene="rpsN"
                     /locus_tag="VBC94_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEO6978673.1"
                     /translation="MAKKSKIAKFQKQQKLVEQYAELRRELKEKGDYEALRKLPKDSN
                     PNRLKNRDLIDGRPHAYMRKFGMSRINFRNLAYKGQIPGIKKASW"
BASE COUNT        14228 a         8133 c         6792 g        13295 t
ORIGIN      
        1 ttactgactt atgttcaatc cccattctat aattcaatta aaaaataaaa aggcgaggtt
       61 tacacctcac cttcaacata ttatttaaag aattctttat tggtttaagt tttcgttgga
      121 gaaaaattca gtgtctccga ttttatattt ttaaatatta aaatactatt tagggagtcc
      181 ctctttatgt tttattacct ttggtaacac caaaacatac acgaactttt attcatatgt
      241 gttaccattt tacatctttc atattgagat atttgattag caatacctcc ttgactaaca
      301 aaaattacta acacatctag gggtataccc tagtttatat ttgttcatga ttacctcaag
      361 cgagcatgcg ctaatggtaa tgaactatta ccacattgtc tgtcttcctt aaattatata
      421 aggacttatg tgtcaccgtt actgtagcga cctacatcta aacctaacat aagtgcccct
      481 ccaactattt ctaggaaatg aagaaaactt caaatccttg tgactgtttg caacatcaat
      541 gcattgataa caattttagt gtatcgccat tcttatagcg acccatatta aaaattaaca
      601 taagcgctac ctccattaac taaagttagc ttgcctacca atctttcttt tggagactag
      661 tctccattct tatacctaga gatattgtaa tagatcatgg ataacctcct tgatttaaac
      721 tttatcttta taatttaatt atagtgtaaa cgcttcctta agtcaagaaa aaaatattga
      781 tgcaacattt ttcttcatca ttaagtacga tctagtcaac atacctcttt gtcaaatcct
      841 ctttctttga tacaattaat ctatgataga tttaaaagac tatggaattg agatgtggcc
      901 tgaagataga atcgcttctt ttcgtcgcac acttttaggg tggtatgacc aagaaaaacg
      961 tgatctccct tggcgaagaa ctactaatcc atactatatt tgggtttctg aaatcatgtt
     1021 gcaacaaaca caagttaata cggtaatccc ttattataaa cgttttctag agtggtttcc
     1081 tcaaataaaa gatttggcag acgctcctga agaacaatta ctaaaggcat gggagggact
     1141 aggctattat tctcgggtgc gtaatatgca aaaggctgca cagcaagtga tggttgattt
     1201 tggtggcata tttcctcaca cttatgatga tattgcttca ttaaaaggga ttggtcctta
     1261 tactgcaggt gctatcgcca gcatctcatt caacttacca gagcctgctg ttgatggcaa
     1321 tgtgatgcgt gtcatggctc gtttgtttga agttaattat gacatcggtg accctaaaaa
     1381 tcgtaaaatt tttcaagcta ttatggaaat actaattgac cctgacagac ccggtgattt
     1441 taaccaagca cttatggatc tgggtacaga catcgaatcc gctaagacac ctagacctga
     1501 tgaaagccct attcgtttct ttaatgctgc ttatttgaat ggtacttata gtaaatatcc
     1561 cataaaaaat cctaagaaaa agcctaaacc aatgcgaatt caggcttttg tcattcgtaa
     1621 ccaaaatggt caatatctac tggagaaaaa tactaaaggg agacttctag ggggtttttg
     1681 gtcatttcct attatcgaaa cgtcacctct ttctcaacaa ttagatttat ttgatgacaa
     1741 tcaatccaac cccataatct ggcaaaccca aaatgaaact ttccaaagag aatatcaatt
     1801 aaagccccaa tggactgata atcactttcc aaatattaag cacactttta gtcaccagaa
     1861 gtggacaata gaactcatag agggggtggt caaagcaaca gatttaccta atgctcctca
     1921 cctaaagtgg gtagcaattg aggacttttc tctgtatccc tttgcaacac cacaaaagaa
     1981 aatgctagag acctacttaa aacagaaaaa tgcttaatca caagtgaact atgacggttc
     2041 actttttgtt tacaattcct ttacgccgct gtaaagtttt cctcagatta tcttttaata
     2101 ccctataaat agtggtctat ccctaatgtc aaatacattt gatagacccg ttagcttatc
     2161 atccttatac taatattatg aaaggagata tttatggaaa ttggtcagca aattattcgc
     2221 taccgtaaac aacaagcgct ctcgcaagaa gaattagccg agaaagtcta tgtaagccgt
     2281 cagagcatat ctaactggga aaatgacaaa acttacccag acatccatag cctcttactc
     2341 ctaagtcaaa tttttcaagt gagcttagac caactaatta aaggagacat tgagaaaatg
     2401 aaatatacca tcactcaggt tgataaaaag aattttgaac gagataccaa agtaatggtg
     2461 acattgatga tactattaat gatttcaagt tatccattag tctatttctt agagtggcta
     2521 ggacttggca tttttgtttt attatcgata attactatga cttatgctaa tcgtgtggaa
     2581 cgttttaaga aaaaatatga tgtgcaaact tataaggaaa tcctagctgt ttcaagtgga
     2641 aaattattag acgaaatcga aaaacgggaa gaaagagcaa aacttcctta tcagaaaccc
     2701 cttattgtca ctgtattttt cctcattact gttgccacct tttttgcaag tcgttttatt
     2761 ttcacatggt tgttccattt aagttagtag ataataaaat aaagctcagc gtgacactgg
     2821 gctttatttt atttaactca attcagcaat aatagctttc agttggtctt tagtatgaac
     2881 acctgcaact tgtttaacca cttctccgtc ttttttaaac atcaatgttg gaattgacat
     2941 aataccaaac tgacgggcag tttccggatt ctcatcaaca tccattttca aaattttgag
     3001 ttcatcttca tcgatttctt gagataactg ttctaagatt ggtgcttgca tacgacatgg
     3061 accacaccaa gttgcccaaa aatcaatgag tactaaacct tctttagttt cttctacaaa
     3121 tgtagcatct gttacttcta atgccatctt ttttctccat ttcatcttaa tgataagcct
     3181 attgtacaag aatttagttg tttttgaaac tatttgctac gaactcgcta aataaataaa
     3241 caaagcccta aaatcaagcc aataaggtaa cctacaataa catccttagg atagtgaata
     3301 cctgctatca cacggcaaat tgccaataga accgataata ttaaacaaac tattccaaag
     3361 taaacataat atcgtaacaa acacatacta atcatcgtag ctgaaaaaac atgtctgcta
     3421 ggcattgacc ttccctcagt atccttataa attaaaggtt taatattcca tttttcatat
     3481 ggtcgtggaa aatcaaggcg tttcctaatg taagacaaag ctataaagcc aacagcaggg
     3541 ataattaata gccttaacca taattgaaat gtcatccctt gccaaagagt aaatatcaga
     3601 aaagatggat agagtatgta catcattcct gtgactattt ttagtaaaaa atttaaaaga
     3661 attattaact gtggggattt tctaaaaggt tgagatagtt tggcataaaa ttgttcatat
     3721 gattccataa tttaccctaa cgttacaatt gtggcgccag atccccctgc attttgtggg
     3781 gcataagcaa aatgcttaac gtgcttatta cgacgaagat attttgtcac tccctcacgg
     3841 ataacgcctg taccaatacc atgaatgata tcaacttgtc ccatattgtt aagcaatgct
     3901 tgatcaataa aattatctaa ctcttgcata gcttcttcgt atcttttacc tctaagatca
     3961 agtcgagctc ttggtccaga actatcagcc tttttaacca cattaatctg tttacttttg
     4021 actttctgtt cttcttggac tctaacaagg gtaaattcat cttgtgttaa tgtcattttg
     4081 ataattccca cttgtgcttc ccaacgtcca tcttttaatt gactagttaa ggtacctcgc
     4141 tgtccatagc tagtcactat aatatcatca ccaattctag gagcacgagc tgctttaatt
     4201 tttttagcct tatttaagac tttatttttt gataaatcaa cttgaggtgc taattttttt
     4261 atttgtgcct tagcatctat aatttcgtga ggttttaatt ggctcttatc attgagtttt
     4321 tttaagatag tatcactctc attcaaagcc atatctacaa tttcttgagc ttcttgatag
     4381 attttttcta actctttatc gcgctcatgt gaaaattcat tatagagttt cttaaccgca
     4441 cgattgaatt tgaggttttc ttgttcaact tctttaatat gatccagtct tctacgtgtc
     4501 tcaagtgtct gtgcctctaa ctgttcaata atacggttaa catctgagtc agaatctgtc
     4561 atctgcttag cttgtttaac aataaatgga gctaaaccaa ggcgagaagc aatttcaaat
     4621 gcatttgatc gtccaggaac tccttgcata aagcgatacg tagggctaag cgtttcggca
     4681 tcaaattcca tgctcgcatt ctctacaaaa tttgtctcaa tcccataagc ttttaattct
     4741 ggatagtgcg tggtcgccat cgttttgata ttacttaacc taaggtgttc taaaatagcc
     4801 atagccaaac tagcaccttc ttgaggatcc gttcctgctc ccagttcatc aaagaggact
     4861 aaactattgt ggtcagcctc gtttaaaata ctgactatgt gcgtcatatg actagaaaaa
     4921 gttgatagac tttgttcaat agattgctca tcgccaatat ctgcaaagat attgttaaat
     4981 actgcaattt tactaccttt atccgctaat actggcaaac cagactgtcc cattaattgt
     5041 gctaaaccga gtgtttttag cataatcgtc ttaccaccag tattgggacc agtgatgaca
     5101 attgcagtta aatcttgatc aaaatgtaag tcattagcca cagggttact taacagagga
     5161 tgacgaacat tgattaatgc taacgtgctg tcattagaaa tctcaggtat cgtcgcctta
     5221 ttatcagaca taaaaagata tttagccctt acaaaatcaa gatgcccaag aatccatgca
     5281 ttatttctaa tagtggcgac atggggtctt aacaagtctg aaaatgcgtg taaaatacga
     5341 ctttcttcat gacgttcgtc agctctaagc tgcgttatct cttcgtttag tgtaactaca
     5401 gcacgaggct caatataaac agtacttcct gaagaagaga tgtcatgaac cacaccagaa
     5461 atacgattcc gataagtatt ttttactggt aggacacttc gtccactacg actagcgatt
     5521 agattctctg ataaaagctc tgctttttcc ttaagcatat cctgtaaaat ctgacgaacc
     5581 cgtcgttcac tgtttgttaa ttgacgacgg atacgctcta attctggact cgcaaaatgt
     5641 tctaaaaaac caccatcatt gatagcttga aaagaccctt gcagattagg gaattgttcc
     5701 aaattttcaa acaaacgatc caaagattgg aaagaaacat tatccaaatc agaataaaag
     5761 tgaatcatat ccgaagaact ctgtaaaact tttttgatag ccaaaagttc ttgaatatta
     5821 agatcagctg aaagctctaa gcgttttaaa ctctcagaga ttgaacttag gctgactatg
     5881 ccaaatgagt gatgttcaac aaaaatctgt tccatgtcag aaatttcatt aaaacttttt
     5941 tctattttag gagcctctgt catcggctcc agctctgata attcttcttg tgattgttct
     6001 gtcttgagat aaggtaatat caattcctta actttgttaa attctaactg ttctaaaatc
     6061 ttgttattca tactcttcat tatacctaaa tttatcacaa aatgacaacg ttaaaaaggt
     6121 tggaactctt actccaacct tactaaacat tatattcaaa gacctaccgc ccaagtcctt
     6181 accaaaaact gtgagaagaa tggtaagtcg ttaattaaaa atctaacaaa cagactattc
     6241 cataactttt cttgaacaaa tgacataggg actgttgcca gaatagtcaa acacatactc
     6301 ataaataaga gacaaaccag aacggctaaa aaaccactaa taatattatt ttgcaagcta
     6361 tcaaaatgtt caactggtgc taaatgtagt aaaacaccta acaagcgacc tactaagtaa
     6421 gataaactgt agataaaaac aaaggcaaca cctgcataat aaacatgact caattgaaat
     6481 ttgctaatat ctttaaaaaa gtaaacttct actccttgta caggattaga atacggtacc
     6541 cataacgtaa tctgactagc taattcttgg taaaattgac tagcaatcat caaagatacc
     6601 attgacatta acacgtaaaa gacttgtaaa aaaatacctc tactgtagcc aatataaaaa
     6661 tgccaaataa caataatcag caaaagcaat gatagcatga ttaatcctca ctcacttttt
     6721 ctttaatgtc cgaaatcgtt ttggagcgta acttattgag ctcatcttcc attttttcaa
     6781 tgtcaatttc tcgacttagt tgcactgata aagcattaat tgccatgaga atagcaatcg
     6841 tttcattatc agcattaggt aatttttctt taatagcttc atacttttct gtagctacac
     6901 gctcaatctc ttccataaaa aggttgtctt tatctgtcgt caatgtgaga ggcttatctc
     6961 caaagacaaa tttataacga tttttctgac tattatttat ttttgtttcc atactatacc
     7021 tctgctagca ttataccgta aaatagggct ttcgtaaaat tctacccctt atcattactt
     7081 gtctattccc ttattcctct tttttatgat agaatattgt ctatgaacac aatcgtcatg
     7141 caagcagata aaaaattgca agaaaagata agaaccgatt tagctcaaca tcatataagt
     7201 aataacaatc cttatgtggt attttcagct aaaatatcag gagctactgt tcttttatat
     7261 acttctggaa aacttgtctt ccagggttct aacgcttcac atatcgccca aaagtatggt
     7321 tttattgagc aaaaagaaag ttgttcctct gagagtcaag atattcccat aattggtact
     7381 gatgaagtgg gaaatggctc ttactttggt ggactggcag tggtagctag ttttgtaact
     7441 cctaaggacc atgcttacct caaaaaatta ggtgttggtg attcaaaaac acttactgat
     7501 caaaaaatta agcaaatcgc gcctcttttg gaaaaagcaa ttccacacaa agcattattg
     7561 ttatcgcctc aaaaatataa tcaagtggtt agtccaaata acaaacataa tgctgtctcc
     7621 gtaaaagttg ctttacacaa tcaagctatc ttccttcttc ttcaagatgg ttttgaacca
     7681 gaaaaaatcg ttattgatgc ttttactagc tccaaaaact atcaaaatta tttgaaaaat
     7741 gaaaagaatc aatttaaaca aacgattact ctggaagaaa aagctgaaaa taaatatcta
     7801 gctgtagcgg tcagttctat tattgcacgt aatctattct tagaaaacct taacaaatta
     7861 agcgacgacg taggatataa acttccaagt ggagctggtc accaatctga taaggtcgct
     7921 agccaattac tcaaagctta tggtatctct tcgcttgaac actgcgcaaa acttcatttt
     7981 gccaacacca aaaaagcaca agcattatta aaataaatag cagggaaaat tatgaaagaa
     8041 tttattaaag aatggggtgt ctttatcctc atcctctcac tttttttact atcgcgtatc
     8101 tttttatggc aattcgttaa agttgacgga cactccatgg atccaacttt agctgacaag
     8161 gaacagctag tagttctcaa acaaacaaaa atcaatcgat tcgatattgt agtggctaac
     8221 gaagaagaag gcggccaaaa gaaaaaaatt gttaaacgtg tcattggtat gccaggtgat
     8281 gtcatcaaat ataaaaatga caccttaact attaacaata aaaaaacaga agaaccttac
     8341 ctcaaggaat atactaaatt atttaaaaag gataaattac aggaaaaata ttcgtataac
     8401 ccacttttcc aagacctagc acaaagctct accgctttca ccactgacag caatggcagc
     8461 agcgaattta ctactgtcgt gcctaaaggc cactactatc ttgttggtga tgaccgaatt
     8521 gtctctaaag atagtcgtgc cgtcggttcc ttcaaaaaat caacgattgt gggagaggtt
     8581 aaattccgct tctggccaat tcgtcgtttt ggaactatca actaaaggaa attaaaagtt
     8641 cacctgacaa aagttgaaat agcatattat attgaaaata gggagtgaca aatccattta
     8701 aaatagggat ttacaatcac tccctttaac tgtttattaa aaaggaaggt acaatggaag
     8761 tattttttac aggaaccatt gaacgcatta tatttgaaaa tgccagcaat ttttttaaaa
     8821 tattacttct ggaaatcgaa gatacggata gcgattttga tgatgtagaa gtaattatta
     8881 ctggtactat ggccgacgtt atagagggag aagaatatac tttttgggga acactaaccc
     8941 aacatccaaa atacggcgag caactacaaa gtgtacgcta tgaaagagcc aagcctactt
     9001 caggtggcct tgtgaaatac ttttctagcg aacaatttaa aggaattggt aaaaagactg
     9061 cccaacgcat cgtagaactc tatggagata ataccattga taaaatcctt gaatcccctg
     9121 aacagttaag tacaatctca ggactatcaa aaattaatag agaagcattc atcgctaaac
     9181 tgaaactcaa ttatggaaca gagcaagttt tagctaaact cgctgaatat gggttaagca
     9241 atcgtgctgc tattcaaata tttgatcact ataaagaaga atcacttgaa gtgataaacg
     9301 aaaatcctta tcaacttgtt gaggatatcc aaggtatcgg ttttaaaata gctgaccaac
     9361 ttgctgaaca agtcggtatt gaaagtgatt ctcctaaacg ctttcgtgct gctattattc
     9421 atactttagt tgagtcttct atggagcaag gagataccta tattgaggct cgtactctct
     9481 tggaaaaaac cattactctc cttgaagaag ctcgtcaaat cgaactagat ccttcaattg
     9541 ttgctaagga attgacaaat ttaattgcag aagataaagt tcaacatatt ggaactaaaa
     9601 tttttagtaa tactctattt tttgccgaag aaggcatcaa aaaaaactta caacgaatac
     9661 taaatcagcc tcttgataaa caactaaatc acaaagatat tgaccgtgaa atcagagata
     9721 ttcaaaaatc tttaaatatc cactacgata atatccaaga aaaagctatt agagaagctt
     9781 tactaagtaa ggtatttatc ttgactggtg gcccaggaac aggtaaaaca acagttataa
     9841 atggtattat tgaagcctat tccgagttac atcatattga tttaaacaaa aatgatattc
     9901 ctattgtact tgctgctcct acaggtcgtg ctgctagacg catgaatgaa cttagcggtt
     9961 taccgagtgc tactattcat aggcatttgg gattaaacgg agatagtgac tatcaaagtc
    10021 tagatgacta tctagattgt tccttaatta tcattgatga attttcaatg gttgacactt
    10081 ggcttgctaa tcaattattt gacgctttgg actcgcacac acaagttatt attgtcggag
    10141 atagtgatca attaccgtct gtaggtccag gacaagttct agcagatctt ttaaatataa
    10201 atgcacttcc acacgttaaa cttgaaaaga tttttagaca atcagaagaa tcaactattg
    10261 tgactttagc taatcaaatg agacagggct ttttgccaga ggactttaca gctaaaaaag
    10321 ccgatcgctc atactttgaa gcatctgcta atatcatccc aaatatgatt tctaaaattg
    10381 ttcaatctgc acttaagagc ggtattgaag ctcatgaaat tcaaatttta gctcctatgt
    10441 atcgcggcca agcaggtatt aataatctta accttatcat gcaaaatctc ctcaatccat
    10501 taaaagataa taaccaattt acttttaatg atataaattt ccgaatagga gataaagtgt
    10561 tacatttagt caacgataca gagcttaatg tttttaatgg ggatattggt tacataacgg
    10621 accttatacc agccaaatat actgagagca agcaagatga gatttatatg acttttgacg
    10681 gtcaagaagt tatctatcaa cgcaaagaat ggcttaaaat aacactagcc tatgctatga
    10741 gtattcataa atcacagggt tctgaatttc aagttgttat tctgccaata acgagacaga
    10801 gtggtcgaat gttacagcga aaccttattt atacagctat tacgagatct aaaagtaaac
    10861 taatactttt aggagaaatt ggtgcatttg atttcgctgt taaaaacgag ggggcaaagc
    10921 gcaatactta tctgatagaa agatttgaaa acaagcaaga aatagctaat tctcaaaaaa
    10981 ttgaagatag ctcaatagat caaaagattg acaacacaat aattaatacg tccattccaa
    11041 aaacagcaac accaattgag cagactaatc tttcaaaaat aacttatcgc ctaactgaag
    11101 aaaattattt aacaatcgat ccaatgattg gtattaatca acaagatata agtgcaattt
    11161 ttgatagtaa ataaattttt tcataaaatc ttgaaaactc ttccaaatat gttataatat
    11221 cattgtatta agaacgtagt tcaaatttaa tacttttatt aaggtttatt tagaaaaagg
    11281 agatattatg gtttcatatg aaaaagtccg ccgctctctg cgcacagcta caatcactat
    11341 cattgtatta aatagtttgt ctttagtatt cagattgttt acaggaattt cagtgcagct
    11401 agccaaaact gaaattaata aagggaacac aggtaatctt cctaaagaac atatcgaagc
    11461 agtcttgagc gccacaacac catttatgct atttgtcact gctttaatag tattagtcaa
    11521 cattgccatt gttattttct gtattaaaaa tttaagagca attaaaagaa atcaaatggt
    11581 taattacctt ccttattacc ttggcttcgc cattactgta ggacttgtta ttttgggttt
    11641 tctaactact aaagcaccat gggcaatagc tataaatatc gtcttccaag ctattttcgg
    11701 tcttctttat ttccatgctt atcaaaaagc acaaaaactc aacgaacgtg atcttgaaga
    11761 gactaactaa aagtaaaagc actagaatct agtgcttttt tcttttcaaa gagagttaaa
    11821 tatagtcatc gtcccaactt ttaaaaatct aaatagatag cttcatgttc attttccagg
    11881 gtcgttactg taacacctaa gagtcgtatg ccaaaaactg actcttctaa ggtatcaaat
    11941 atcgctttag caacttgatc aatgatttga aaatcttgag tatactcttc taatgtcata
    12001 cgtttcgtca atgtttcaaa atccgcatat ctaactttta aaacgattgt cttaccaact
    12061 ttcttatttt tctccagaga agcaacaact ctctgaacgt tctttgaaat ctcagctttg
    12121 atatctgctt cgttatatag taatttccca taggttttct cacttcctat tgatttacga
    12181 actctattag gttttaccgg ggaagcatta atccctcttg ccttacgata aaggtcatac
    12241 ccgaaacgac caaacatatc aattaaagaa atttcagata aggaaaggag gtcctcacca
    12301 gtataaaccc ctaaagcatg taatttttcg acggagcgtt taccaacacc atgaaatttc
    12361 tctattggta agggcttcaa gaaatcttga gcttgatctg gtaggatgaa ggtaagtcct
    12421 tttggtttct caaagtcaga agccaatttt gccaaaaatt tattgtagga aatacccgct
    12481 gaacaggtca aatggacatc attccaaata tcgtattgta tcattttagc aagtttaaca
    12541 gctgatttta ttcccatttt attttcagtt acatctaaat aagcttcgtc tatactcatc
    12601 ggttcaacca aatccgtata ctttttaaat atatctcgta cttccattcc tacttggcga
    12661 tatttttggt aatttcccga aataaagata gcttgagggc atcgttcata cgcttctttt
    12721 gaagacatag ctgaatgaac tccaaacttt cgagcctcat aattacaagt agaaacaacc
    12781 cctctgccgc cggtttttct gggatcagaa ccaataataa caggctttcc tttaagagag
    12841 ggattgtcac gctcttccac agatgcaaaa aaagcatcca tatcaatgtg gataatttta
    12901 cgcgacgtat catttattaa aggaaaaatt aacattgttc tctcccccat tctctctact
    12961 ccattataac agattacaac ttaatgatac agattaaaaa aactgtatta taaaagaatc
    13021 ataattttgt caagaaatat gcttgcgaaa acgtttactt tttcctaaaa tggatatgta
    13081 aacaaaatta aatctgtcac aatgtttaag gagaaaacta tggcaacagt taaaacaaac
    13141 acagatatct ttgaacaagc ttgggaaggc tttaaaggtg ttgattggaa agaaaaagca
    13201 agtattgctc gcttcgttca agcaaactat gcaccttacg atggagatga aagcttctta
    13261 gctggagcaa ctgaacgttc acttcacatc aaaaaagtga ttgaagaaac aaaagctcac
    13321 tatgaagaaa ctcgcttccc aatggatact cgtgttgctt caatttctga attaccagct
    13381 ggttttatcg acaaagataa cgagcttatc tttggtattc aaaacgacga gcttttcaaa
    13441 ttgaatttca tgcctaaagg tggtatccgt atggcggaaa ctacacttaa agaaaatggt
    13501 tatgaacctg atccagctgt tcacgaaatc ttcactaagt acgctacaac agtaaatgat
    13561 ggtatcttcc gcgcttatac ttctaacatt cgtcgtgctc gccatgcaca cactgttact
    13621 ggtcttccag acgcttattc acgcggacgt atcattggtg tatatgctcg tctagctgtt
    13681 tacggtgctg actacttgat gcaagaaaaa gttaatgact ggaatgcatt gaatgatatt
    13741 gatgaagagt caattcgtct tcgtgaagaa atcaaccttc aataccaagc tcttggtgaa
    13801 gttgtaaaac ttggtgacct ttacggcgta gatgttcgta aaccagccat gaacactaaa
    13861 gaagccattc aatgggtgaa cattgctttc atggctgtat gtcgtgttat caatggtgct
    13921 gcaacatcac ttggacgtgt tcctattgtc cttgatatct ttgcagaacg tgacctcgct
    13981 cgcggtacat tcacagaatc agaaatccaa gaatttgttg atgattttgt ccttaaactc
    14041 cgtactgtaa aatttgctcg tactaaagct tatgatgccc tttactcagg tgacccaacc
    14101 tttatcacaa cttcaatggc aggtatggga gctgatggac gtcatcgtgt taccaaaatg
    14161 gactatcgtt tcttaaatac acttgacaat atcggtaact caccagaacc aaacttaact
    14221 gttctttggt cagatcaatt accatatgct ttccgtcgct actgtatgtc aatgagtcat
    14281 aaacactctt caatccaata tgaaggtgta tcaacaatgg ctaaagaagg ctacggtgaa
    14341 atgtcatgta tttcatgttg tgtatctcca cttgatccag aaaacgaaga caaacgtcac
    14401 aaccttcaat attttggtgc tcgtgttaac gttatgaaag cacttcttac tggtcttaac
    14461 ggtggttatg atgatgttca taaagattac aaagtatttg acatcgaccc aattcgtgac
    14521 gaagtactca actttgacac tgtaaaagct aactttgaaa aatctcttga ctggttgact
    14581 gatacttatg ttgatgcgat gaatatcatc cactacatga ctgataaata caactatgaa
    14641 gctgtacaaa tggcattctt accttcacat gtacgtgcta atatgggatt tggtatctgt
    14701 ggttttgcaa atactgtcga ttcattatct gcgattaaat acgctactgt taaaccaatc
    14761 cgtgacgaag atggttacat ctacgactac gaaacagttg gtgacttccc acgttatggt
    14821 gaagatgatg atcgtgttga ctcaatcgct gaatggttac ttgaagcctt ccatggacgc
    14881 cttgctaaac acaaacttta taaagatgct gaagcaactg tttctcttct tacaatcaca
    14941 tcaaacgttg cttactctaa acaaactggt aactcaccgg tacataaagg agtatatctc
    15001 aatgaagatg gtagtgtaaa cctttctaaa gtagaattct tctcaccagg tgctaaccct
    15061 tctaacaaag ctaaaggtgg ctggttacaa aacttgaatt cactttctaa acttgatttc
    15121 gctcatgcaa atgatggtat ttcattgaca actcaagttt ctccacgcgc tcttggtaaa
    15181 acatttgatg aacaagttga taacttggta acagtccttg atggttactt tgaaaacggt
    15241 ggtcaacacg ttaacttgaa tgtcatggac cttaaagatg tctatgacaa gattatgaat
    15301 ggtgaagatg ttatcgttcg tatctcaggt tactgtgtca atactaaata tctaactcct
    15361 gagcaaaaaa ctgagttaac acaacgtgtc ttccatgaag tactttcaat ggacgatgca
    15421 ttaactaact agtttgtatt cgatgaagta ggtttgatta tatcaaacct acttttatct
    15481 atattatcaa tagtactaat tacttcttta tataacctca aggaggggcc taatgttaaa
    15541 tcacaaattt cttcaagttc taaaatatga gggtgtcgtt tcgataacaa gctggaccga
    15601 attagctcct cacgttacaa acacttggaa tagctacctg actattacag atgaccagcg
    15661 tatattagct cctgcagcag gaatgactca tcttgaaatg atctcaataa taattctaaa
    15721 attattatga ctctgggcag ccgtgaagtc gagggtcgtg atggttatca aggtacaggc
    15781 tttagaattg aaggaactgc taagctatta gaagcaggaa gcgattttga aattgttaaa
    15841 gaaaaatacc cttttctgcg taaagtattg gaagtaactc ccataaatgt tatccaactt
    15901 ttataaaaaa cttctcctag aaaattctag gagaagtttt ttattcaaag tacgatgtta
    15961 tcgcctcttg tacatgtcgt cgtttcttaa tccaaagtgg acggtcatca taggaaaagg
    16021 ttctgtttgt caaaaaaata gctgctgcct gcttttgata atttagtgca ataaaaggac
    16081 cagtataacc agtatggttt atccaatcct tatctatatc ccaagctaat gatcttgatt
    16141 tactcaaact ataattattc cacaaaggct tcgcaaaatc atcttttaaa taatgaatag
    16201 aaaatctttg taaatcatca atagttgaaa ataaacctgc agaaccagta tgctttccta
    16261 ataccttagc tttaggatcg tgtacaatac catcgttaat accggctaca gttggaacag
    16321 cttctactct aggaccaaaa gaggtttctt tcatctgaaa aggagtgaaa atataacgtt
    16381 tgaaaagttt atccaaacta tcccctaata cctcttctaa cataaaacca agtaaaagaa
    16441 aattaatgtc agtgtattta aacgatttat cttccaaaac cttgatatga ttaatggcat
    16501 ctttcagctg agtagcattc aactgatcac gatttggaat aaaagggtca attccactgc
    16561 tatgtgtagc taattgcctt acagttaacg tctgatgatg aaacgtaggg taataatacc
    16621 ttaatggctt atcaatatca atagtccctt gataaactaa ctttgctagt aaagttccca
    16681 cgccgacaac ttttgataca ctggctagat catagaccaa tcccgcttta actttctcat
    16741 tgccatcaat tgtccccaag taatgttcgt gccactctcc attttcataa atagctaagc
    16801 ttgcaccatg ataaagatta tctttgattt gctccttaat gagttctaaa acggtattag
    16861 ttttaatcat tcttttgaaa tccaaatctc aatattacta gtgtcagaga tgactaaaat
    16921 cttttcttta gaatcaaggt aaacatcaag tccccatgat tcaaaagttg atttcaagtc
    16981 atttaaatta gtatcttcat taacacagca ttctagtatc tcaatatccc aagtttcatt
    17041 tggagcaatt tgcaaatctt gtccctgtgc ttctttgaaa gataaattaa ttgggaactt
    17101 cccagcaaat actttatcgt aaaaagcttc agctttcacg gcatcaggta cattcaaact
    17161 aatagattgg aaagcaaagt ttgatagacc tgtagtgtca tctttttttt ctaactctgg
    17221 gcgctcaatt gccactaatt gtgacaaatc atcttcagca tgaagtaaga aaaggtctcc
    17281 ctcgggcgaa actgtttcat aggcataacc attttgacct tgatatactt gaatggcttg
    17341 cgcaccattt gccaaaagct tttcaatatc ttttgcatct tgagacttaa caataatctt
    17401 tgctaacttc ttagtaccat tcaccgcacg agtacgataa gttggggatt cctcaataat
    17461 aaagctagcc tctttgttct gccatgctga gaaaactgcg attgcatttt cttcacttat
    17521 caatttaaat ccaagagact cctgataaaa tgcaatattc aagtctctat tattaacacg
    17581 taatactggg tgtttaaatg tcaatgaatg aaataacgtc ataaattcct ccttgttcag
    17641 tctagtctat tttagataat tttagataat ttagcaagaa tctttgtgct ctcaatacca
    17701 taaagtcaaa ttgacagttt cactattttt ttgtagactg gatattatga atgaaacact
    17761 actattacat gggattcagc ttattctgat tatcgccatg attatcactt tttatcagat
    17821 tgttagacat attaggtctc aaaaaataaa tcctttcaaa cgatttttca ctggtttgtg
    17881 gatcggtttt gtaactgatg ctctcgatac tcttggtatc ggctcttttg caacaacgac
    17941 cacatttttt aaactaacta agcttgttga agacgatcgt aagatacccg caacaatgac
    18001 agctgcacac gtacttccag ttttgttgca gtcactctgt tttatttttg ttgtaaaagt
    18061 agaggcttta acactaataa caatggctgg tgcagctttt attggggctt ttgttggagc
    18121 taaaatgact aaaaattggc acgctcctac tgttcaacgt attctaggaa cactgctcat
    18181 cacagctgct attattatgc tctatcgtat gatcactaac cctggcgctg gtatttcaga
    18241 ttcagtgcat ggtctacacg gcatttggtt atttgttggt atcgtcttca attttattat
    18301 tggtgtatta atgactatgg gactaggtaa ttatgctccc gaacttatct tcttctccct
    18361 aatgggactg agtcctgcag ttgctatgcc tgtcatgatg ctagatgcag ctatgataat
    18421 gacagcttct agtacacaat ttatcaagag tggtcgtgtt aattggaacg gctttgccgg
    18481 tctggttact gggggaatat taggcgttat tgtcgctgtt ctcttcttaa ctaaccttga
    18541 tcttaacagc ttgaaaacac tggttgtagg tattgtttta tttactggag caatgttaat
    18601 acgttcttcc tttaaaaaaa ctatgcccaa aagtcatcat taaacgaaat cagctagcaa
    18661 gactggctga tttttatatt taaaacaaaa aaagaccggt aaaccgatct ttctttaact
    18721 attaaagata taatagtttg aacaaggcta cagcgagtat acttgctacg attggtgcta
    18781 aaactggcac ccatgcatac caccattttg aatcttcttt agcttgtcca agaatttgtt
    18841 ttggtaataa gcggtgaaca atacgtggac caaggtcacg cgcagggttc aatgcaggtc
    18901 cagtcggtcc acctaaagaa gcaactagag tcattaccaa gaaaccaata cctaagtgag
    18961 ctacagcaag tgaaccagtt acgtatggtg aaatccttgt cgctgctgtc gtttggtcat
    19021 aaccagcttg aacaagttta ccaacaagtt ctacaccaaa atagttcttc gttaaagcca
    19081 aagctccaaa gaaaagaaca aaagaaccaa cgaattcatt taagaaacca ttaatgtaag
    19141 ccgctttacg acttgatttc tgtccatcat ctaatgcaga aatagttgaa aatgatccaa
    19201 gaacatgatt tggattttct gtcttaacaa agtaaggttg ataaaccata acgacaacta
    19261 actgtccaaa tattgctcct aaaatttgtg caagaatgta ttgtccaacg tgtgcccaag
    19321 ggaaaagacc tgaaaatgcc aatccaagtg tgaaagctgg gttaatatgg tttccagaga
    19381 cattaccaaa cataagcgct ggcatcataa cacctaaacc ataaccaatt gcaataataa
    19441 tccaacctga attatttcct tttgtgcctt ttaagtcaac gttagctaca gccccatttc
    19501 ctaagatgat aagaaaagcg gttgcgataa attctgtgat atacttcaca gtccaagtga
    19561 tttcgatcaa tccttgtttc ctccccaaaa aatatttaca acattaacca ttctaccaag
    19621 taagcttaat attgtaaagc ttatcacaaa ataaaattaa taaaattttt aaccaaatat
    19681 atttgataaa aaatagagta ctaggttcgg actacctagt actctatttt atttagttta
    19741 taaggttttc ttactatcac gaacgactag catatcaact ttcgaatgac gtaaaatgta
    19801 ttctgaagtt gagccgatca ataggctttc aaaagtgttt aatcctgttg cgcctaccat
    19861 aattaaatct gctttttccc ttatggggat atcctttgcc aaaagagtct taggatttcc
    19921 gatttcagta atttttttaa tctttgtaag gccttttgaa tgtgctaatt tctcaaaata
    19981 ggctagcaca tctgctgact cttgttcttg cctatcaaaa acatagtcac taaagactcc
    20041 ctcactttgg taagcgtgag catctataac atgcgttagc agtaattcag catcattacg
    20101 aagagcaaca tttatccctt tttctaccgc caattctgac tcgtaagacc catcaatagc
    20161 aataagaata cgctcatatt tttgtgtcat agcttaattc ctccctttta tatagtttta
    20221 gtttcaactt gtctagatga ttttaagaaa tatagaatac cacaaacaac aggaataatc
    20281 gctgctatcc agaataattc tctaaaggat tgcacaccag ctccaagaaa accatcttta
    20341 acaagtccca aaatgtaagg tccagcacct aaccctaaat caagacctat catgtaagtt
    20401 gacatggctg tattgaaacg atgttcctca acacctttga ttgatgctgc ttggccacaa
    20461 gacataaagg tgccataacc aaaaccaatc aaagcacctg atagaaggta ggtaacactt
    20521 cccatagcac tccctaataa agcaagtccc aaagttaaga acagataact tggataaagc
    20581 acccacttat ctcccttagc gtccattaat cttcccatag atggtctggt taaagtgatg
    20641 acaagtgcat aaacaataaa gaaataagct cctactgtca tcaaattaat ttctgttgta
    20701 tataattttt ggaaacctaa cacggaagca taggagatac ccatcaaaaa tgcaataatt
    20761 gtgataaaaa ttgctttttt ctcaatgaaa ctatcaatag tccatgattt tgatttagct
    20821 aactgttctg gatttaaagt aatatttttg actgggaaaa caaatgctcc caacactaca
    20881 atcgcaatta aaatactaca taatacaata accattttaa agttaatatg aaggttgtct
    20941 agcataaatg ttcctacaaa aggaccaata gctgcggcta aacttgttga tagaccgtaa
    21001 aagttaatcc cctcacctct tttatcagct ggtatatagg ctgttacaat agtattagtt
    21061 gctgttgaca cgacgccata accaaaacca tttaggaaac gaactaaata cattactccg
    21121 atacttggca tataaaaata agctaaagtt gttagtaagt aaaaaatagc ccctccacgt
    21181 aaaactaact tacgtcctag aacttctaat tgcttaccaa atataagacg agcaatcaaa
    21241 gtccctacaa tataaatccc cgttgctaat cctgcttggc tagtgctaac acctaactct
    21301 ttagtcgcaa taaaagctat gataacggta aacaaataat agaccatata aacaataaag
    21361 tttaaaatag taatgcctat aaaatgtttg ttgaataatt tgtcttccaa tgcaaatggc
    21421 tccttttttc aatttcattt tcataaacat tttacaaaaa tattagaaga tacgatattt
    21481 caatggtata atataagtgc tagtatcaga ctgcattaac aaatgttaag gtggtctatc
    21541 aaacctaaaa catttggagg aagtcatgaa tcatcaaatt ttacaaaatt acattaatag
    21601 tcataatctt ccaattatcg aaaaagacta ccataaatac cttacatttg aaagtttaga
    21661 agaagactat acttatatcc taaaagatgg tattgttaaa caaagtgttc tctctaaata
    21721 tggtactgag ttcaatctgc gttatgtgac aggcttagaa atcacttcta tcctaaatac
    21781 agactactcc caacacatgg gagaacctta caacgttcga atcgaatccg aaactgccca
    21841 cttttacaaa gtacgtcgct ctaccttcct aaaagatatc aataatgata ttgaactgca
    21901 aggatatgtg aaagatttct atcataaccg ccttgaaaaa tctatgaaaa aaatgcaatg
    21961 tatgctcact aatggtcgaa ttggtgctat ctccacacaa ttatatgacc tcagtaagat
    22021 gtttggtgaa gaacgagata acggtgatat ctatattaat tttgttatca cgaacgaaga
    22081 acttggtaaa ttttgcggaa tatccacagg aagtagcgtg agccgcattt tgaaacaact
    22141 aaaagacgat catattattc gtattgaaaa acaacatatc attattacaa atgttgagaa
    22201 attaaaagat catattgtat tttaatataa caatagaaag gatatattat gcgttataat
    22261 caattcagct acattccaac taaacctaac gaagcttttg aagagctcaa aggactaggt
    22321 tttccattaa acaaaaagaa ttctgataaa gctaatttgg aagcttttct cagacattct
    22381 tttttaaatc aaactgatac tgattacgct cttagtctcc ttatcgttga tgcaaaaacc
    22441 gatgctctaa ccttttttaa atcaaatagt gacttaacac tagaaaattt acaatggatt
    22501 tatttacagt tattaggctt tatccctttt gtagacttta aagaccctaa agcattctta
    22561 caagatatta acttcccagt ctcatatgac aatatttttc aaagtctaca tcacttactc
    22621 gcctgtcgtg gaaaatctgg caatacatta atcgaccaat tagttgctga tggtttactt
    22681 catgcagata atcactacca ttttttcaat gggaagtctc tggccacttt caatactaac
    22741 caattgattc gcgaagttgt ctatgttgaa acatccttag atactatgtc tagtggtgaa
    22801 catgatttag taaaagttaa cattatcaga cccactaccg agcatactat ccctacgatg
    22861 atgacagcta gcccctatca tcaaggtatc aatgatcctg ccgcagacca aaaaacatac
    22921 caaatggagg gtgcgctagc agttaaacaa cctaaacaca tacaagttga cacaaaacca
    22981 tttaaagaag aagtaaaaca tccttcaaaa ttacccatca gccctgcaac tgaaagcttc
    23041 acacacattg acagttatag tctcaatgac tattttcttt ctcggggttt tgctaatata
    23101 tacgtttcag gtgtgggtac tgctggctct acgggtttca tgaccagtgg ggattaccaa
    23161 caaatacaaa gctttaaagc agtcattgat tggttaaatg gtaaggttac tgcattcaca
    23221 agtcataaac gagataaaca agtcaaggct aattggtcaa atggccttgt agcaaccaca
    23281 ggtaaatctt atcttggtac catgtcaact ggtttagcaa caactggcgt tgaggggctg
    23341 aaagtcatta tcgctgaagc cgcaatctcc acatggtatg attattatcg agaaaatggg
    23401 cttgtgtgta gtccaggcgg ctaccccggt gaagatttag acgttttaac agaattaaca
    23461 tactcacgaa acctcttagc tggtgattac atcaaaaaca acgattgcta tcaagcattg
    23521 ttaaatgaac aatcaaaagc aattgaccgt caaagtgggg attacaacca atactggcat
    23581 gaccgtaatt acctaactca cgtcaataat gtcaaaagtc gagtagttta cactcatgga
    23641 ctacaggatt ggaatgttaa gccaagacat gtctataaag ttttcaatgc attgcctcaa
    23701 accatcaaaa aacacctttt tttacatcaa ggtcaacatg tgtatatgca taattggcag
    23761 tcgattgatt ttcgtgaaag catgaatgcc ttactaagcc aagaactact tggcattgac
    23821 aatcatttcc aattagaaga ggtcatttgg caagataata ctactgagca aacttggcaa
    23881 gttttagatg ctttcggagg aaaccatcaa gagcaaattg gtttaggtga tagtaaaaaa
    23941 cttattgata accattatga caaagaagcc tttgatactt attgtaaaga cttcaatgtg
    24001 ttcaaaaatg atcttttcaa gggaaataat aaaaccaatc aaatcactat taatcttcct
    24061 ctaaagaaaa attatctcct gaatggacag tgcaaactcc atctacgtgt taaaactagt
    24121 gacaaaaagg ccattttatc agcccaaatc ttagactatg gtcctaaaaa acgattcaaa
    24181 gatacaccaa ccatcacatt cttaaacagc cttgataatg gtaaaaattt tgccagagaa
    24241 gctttacgtg aactcccgtt tactaaagat cattatcgtg tcatcagtaa aggtgtcttg
    24301 aaccttcaaa atcgtacaga cttacttaca attgaggcta tcgagccaga acaatggttt
    24361 gatatcgagt ttagcctcca accaagtata tatcaattga gtaaaggtga taatctaagg
    24421 attatccttt atacaactga ttttgaacat accattcgag ataatgctag ttattctata
    24481 acagtagatt taagtcaatc ttatttaact atcccaacta atcaaggaaa ttaacttatg
    24541 aaacttctca ctaaagaacg gtttgatgat tctcaacact tttggtacca gatcaattta
    24601 ttacaagaga gtaacttcgg agcagttttt gaccatgata ataaaaacat tccacaggtt
    24661 gttgcaacta ttgttgatga tttacaaggt tccggaagtt cgaattattt ctggtatttt
    24721 ggcaatacta ctgatacttc catccttatg attgctcatt taaatcgaaa attctatatt
    24781 caggttaatt taaagaactt tgactttgca ctcaatttaa tagctataaa taattggaag
    24841 agtctcctcc aaactcaact cgaagctcta aacgataccc tagcaatatt tcaataaata
    24901 aggtagaatg gagtgacaaa gcaacgcgag ggagactgat taatgtcatc ttattggaat
    24961 aactatcctg aacttaaaaa aaatattgat gaaaccaatc aactaattca agaaagaata
    25021 caggtcagaa ataaagatat tgaagcggcg ctaagccaac tcacagctgc gggaggaaaa
    25081 cagctcagac cagcattctt ttaccttttt tctcaacttg gtaataagga gaatcaagat
    25141 actcagcaac taaagaaaat cgctgcttct ttagaaatcc ttcacgttgc tacattaatc
    25201 catgatgatg tcattgatga ctcaccacta agacgtggaa atatgaccat tcaaagcaag
    25261 tttggcaaag acatcgcagt ttacactggg gatttacttt tcacagtctt tttcgatctt
    25321 attttagaat ctatggctga tacaccattt atgaggatta atgcaaaatc tatgcgtaaa
    25381 attctcatgg gagaattgga ccagatgcac cttcgttaca atcaacaaca aggtatccat
    25441 cactatttac gtgcgatttc aggtaagaca gccgaactct ttaaattagc tagcaaagaa
    25501 ggagcttact ttggtggtgc agagaaggag gttgttcgtc tagcaggcca tatcggattt
    25561 aacattggta tgacattcca aattttggat gatatcctgg attatactgc agataaaaaa
    25621 acatttaaca agcctgtctt agaggattta gcacaaggca tttacagcct tcctctactt
    25681 cttgccattg aagaaaatcc tgatattttc aaacctattt tagataaaaa aacagatatg
    25741 gctactgaag acatggaaaa aattgcttat ctcgtcgttt cccatagagg tgttgacaaa
    25801 gctcgccatc tagctcgtaa atttactgaa aaagctatta gtgacataaa taagctaccc
    25861 cagagctctg caaaaaaaca gttgctacaa ttaactaatt accttttaaa acgcaaaatt
    25921 taaataataa aaaaacattc cacaatgcta gaaaagcagt tagggaatgt ttttttatta
    25981 tcatttattt atcgcaccta tcaatcatca tagatcacca tcatcagcgg ctttcagctg
    26041 acggtaacgt tggctacttt gagacaattc ttgaggagaa ccttccaact ctaattgccc
    26101 attttctata aataagatac gatcagcatg ttcaatacct tttaagtgat gtgtaatcca
    26161 aactaaggtc ttaccttcca attctttcat aaataccctt agtaaggctt gttcagtaat
    26221 aggatcaagt ccaacagttg gctcatctaa gataacaatt gggacatctt ttagtaagat
    26281 tctagccaaa gcaattctat gcctttcgcc acctgaaaac ctaagtccag cttcatcaac
    26341 cattgtatag agaccatctg ataaatcagt gaccatctct ttcaatccaa ctcgttcaag
    26401 aactttccat acatcttctt cactagcatc ttggtttcca atgcgaatgt tatttagcag
    26461 ggttgtatta aaaaggtagg gcgcttgttg tatcactcca atatagttag aaatgcaatc
    26521 accaactatt gaaacatcag caccgcctag ggtaatctcc ccttgacttg ctttcaagtc
    26581 gccacgaagt agactggcta aggtactctt gccagaacca ctacgcccta aaatagcaat
    26641 tttttctcct tctttaatat ccaaatctaa atgatgcaaa acccatttct cttgtggctt
    26701 atactggaaa cttaaattct tgacggaaaa atcgtatggc ttattaggca actgattttg
    26761 agttgtttca aaatacgttt ctgataattc attaagacga tgaattgaat cagaatattt
    26821 atttgtttct tgagcagcag cagacaaacc agcaaatgcc tctgacaagg gaaatactgt
    26881 tagaacaaat gctgcaatcc agttagcttc gccacctctg tgaccaataa actgattact
    26941 agcccaaata ataaccaaaa tagctagaaa gccaaaaact aattccacaa taagagctct
    27001 acgattatca aaggaacgaa tttttttctg aacagccatg agttcacttt ccgaacgctc
    27061 atgcaaggtg acgtactctt gaccacgttg actaaaaatc cagtctgaaa tacctaagac
    27121 attatccgtc aaatcagtat aaagatgatt ttttaattct ttttcacgag tctgacgagc
    27181 accattagct aaaatcgacc aaagtggaaa aagaaaaatc ataatagcta aataaagcaa
    27241 catcattaaa gcaaaccata gagaaaagaa gcctgttgca ataataataa agctatataa
    27301 tatccacgct ataatcgttg gaaatattgt cctaagatac aaattttgca aataattaat
    27361 atcctccgct aaaagcccca taacatctcc taaacgaaaa tcacgcttca taaagatagc
    27421 attgctttct aaggaatggt aaagcttcaa gcgcagctgt gaagtcattc gtaataccca
    27481 attgtgactc gttaggcgtt ctatataacg aaaaacagga cgtccaatgc caaaagcacg
    27541 tgttagtaca attggaacat atactagaag aatattactc ggtagtgaag ctgacttact
    27601 aatcagatag cccgaattaa acataagagc actggcagaa aagaaagtca taaaaccaag
    27661 gaaaagagca attactaagg aaactttata ttgtttgaaa aatggtttta tccattgatc
    27721 tgtctttaat tctttaaaca aagggatttt aaacatcatt tcctcccata gcatgcttca
    27781 gttgatagag atatcctttt ttagctaata attcctgata agagcctact tctgcaactc
    27841 tccccttctc cataacgact ataacatcca tttggttcaa ccagtgaaga cgatgagttg
    27901 caaagataac taaacgatct gacatcagtg gcaacatttt ctcttttaat tctaactctg
    27961 tttcaatatc taaatgagca gttggttcat caaaaaccat aatacgacga ttttgatcaa
    28021 gaaaagcacg agccagagct atacgctggg cttgtcctcc actaagcgga cgcgctccat
    28081 taccaatacg cgtttctaaa ccgtcaggta actcgcttag caagctatct aaacctacca
    28141 tatgaatcgc tcttaccacc tcttcgtcac tagcattggg agtatagaag gtaatattat
    28201 cacgtaatga catttcaaaa acataaggag tttgaggaat atagatcatc tgtttatgcc
    28261 aatcttcttg atcaaggtta gtcacttctc taccatccac ttttaaagaa ccattatcag
    28321 gccctaaaaa accactcaga aggttaatta agctagactt acctgaacca ctaactccta
    28381 ctaaagcaac cttttgatgt cctctaaatc gtaaggttac atcctcaaca acacgttttt
    28441 ggtcgtacgc aatagcaatg ttttctaaat ccaaacgact ctctttagac caatcatcta
    28501 tgactaattg ctcaccttta ataccagttt tgtttagaac tttttgaatc gcttgaaatg
    28561 catttttccc gtctaacgtt gcatgataat cactagaaaa atctcttacg ggtaaaaagt
    28621 attcagggga taaaatcaaa attgttaaag ccggtaaaag gtaaatctgc tcatttaata
    28681 atcgtaaacc tagaaaaaca gcaacgatag caatggataa agttgtaaaa aagtctaaag
    28741 caaacgttga taaaatacca attttcaaag tactcattgt tgctttacga aaagattcac
    28801 tagtttgata aatgctttta ccataacgtt tactaagacc aaaatatttg agagtatcaa
    28861 tgcctcgtaa tgaatctaaa aaatgattag aaagtacttg ataggattca tattgtttat
    28921 ctgctttagc ttgagccgca tagcctaaaa taatcataaa gatgataatt aaaggaaaaa
    28981 cgataagtaa aattgcacct gattcaatat ctaaatagaa aatataagct agaataatcc
    29041 atgggataat agacatgtta atcatcttat tcaagactaa acgtaaataa ttttcaacta
    29101 aagaaacgcc atctaatgcc attgtaacaa cattaccagt tccttgttct tgaacgactt
    29161 taggacctag atcaaataac ttatctaata attgacgtct caaaagacta gattgtgctg
    29221 ttgaaaagtc atcaagtttt ctatttttga tgtaatcaat aacatgacgg cctaaatatg
    29281 agaccataaa taacaaaaca taaaccgttt gagaagaaag tttttgccct tcccacaaac
    29341 cagttataga aagactcaaa taataagctt gtccgataat aaagattgct tgaagaacat
    29401 caagtcccgc aagcaatcct aacaacttat gaatacctga caagcgcata accgctttat
    29461 ctagcattat ttttctcctg tcacaatcac tggcaaggta atacgttttc taaagatata
    29521 atatgcccat gctgtataag ctagaacaaa gggaactaga gtaattgcga caatcgacat
    29581 tacttttaaa gtatatggcg ttgaggatgc attttgaatc agcaaatcat atttagggct
    29641 aattgatgaa atcataactc gtgggaatag accttggaac aacagaacaa ccactgaaac
    29701 aagactgaga ccactcgcta aaaatgctgt catctctgct cctttaaaag ttgaagcatg
    29761 cgctaataca gctaatacaa caataaccaa aaccataatt gtcgtaacta tcggatgatt
    29821 gctaaagaaa tctgtcttga aatacaagag aactgcaaac aaggctaaac caagatagag
    29881 tacccaataa agcaactgag cataattacg ggcacgctct ctaattggcc cttctgtctt
    29941 caatgcaatg taatttaaac catgaagata ggctagcaat accattgcaa ctccacccac
    30001 caacgagaat atattaaagt aatcactgaa ttgagctgat aagttaccac tggcatcaag
    30061 tggcatacct tgaatcaaac ttataaacat aatcccaaag aagaatggaa caatagcgga
    30121 accaattgtt aaggtccagt tccaaaactg ctttttctct gcaggaactt tatgtctaaa
    30181 ttcgaaggaa acaccacgaa taattaatcc aaagagaatc gtaaggagaa tcaaatagta
    30241 accactaaat agtgaagcat accagtatgg aaaagaagca aacattgctc caccaccggt
    30301 aagaagccaa acttcattac catcccaaac tgggccaatg gtttctacta cttgatcttt
    30361 ttcatgctca ttatgtgtta aagtttgaac agccatccca accccaaagt cgaatccttc
    30421 aaggaagaag aatcctgaaa ataggaggcc gattaggaaa aaccaaaaga attgtaaagc
    30481 actcatttag tatgctcctt tctcaaaggg atcaattgaa gtatatgcac catgatgtcc
    30541 gtgttcatct tcatactctg gccctttctt caattctctt attgttaggc ttatcatcat
    30601 caccgctaaa ccaccaaaca acaagaaata cacagtattg gaaacaaata atgatgcaac
    30661 tgaaacgttt ggagagacag agtccttaat cttgaaaaga ccatagacag tccaagggta
    30721 tcgtccttgt tctgtcacaa tccaaccaaa tgtattggct aagaatggtg caaaggttgt
    30781 taaagcaaca atccaaagca tccatttgtg tttataaaga attggttttt tcttacgagt
    30841 taaaaagagt cctaaaacag aaacacccat aatagctgtt cccatcgccg ccattgtacg
    30901 gaaaccatag aaaagtaggt taaccattgg atagtagtta tctttaccat acttagcaac
    30961 caattcttta ttagctgtat ccataccttt cacagaacca gatggtttcc cgtaagataa
    31021 aatactcaac atataaggaa tcttaatccc aaaaacttgt ttgtgttctg cttcatttgc
    31081 ccaagcaact acactccaag ctgcaggatc acccgaatct tcataatcac cttccatagc
    31141 agcaaatttc attggttgat cgtgaatcaa ggctttcatt tgtaaatccc caactcccat
    31201 aactgaaatt gaaccaagaa gtgctactac taaaccaata cgaaccgatt tcttataaag
    31261 ttcaaccgtt gtatctttca gctgttctga ttttaagagg cggaaggctg acataccagc
    31321 aaccactgta ccacccatcg taatggcacc aaaaataaca tgggtaaatt catagaagaa
    31381 ctgtggattt tttacaagtg caagaaaatc agtcatttgc gctctaccgt ttacaacttc
    31441 ataaccaaca ggatgttgca tgaagctatt agcagtaaga atccacatcg ctgacattaa
    31501 tgagccaaaa accactagcc aaatgaatgt cacatgtaat tttttactaa tttttttgtt
    31561 atcccatgta aacatccata aaccaaggaa agtagattcc ataaagaaag ctaataaagc
    31621 ttcaatagcc aatggagctc caaaaatatc acctacgaaa cgtgaatagt ctgaccagtt
    31681 cattccaaat tggaactctt gaatgatacc tgtcacaaca ccaaccgcaa aactaaggag
    31741 catgatatta ccccaaaatt ttgtcaattt tttatactct tcattttttg tgataacata
    31801 cattgtctcc ataatagcaa ccactaggca tgtaccaatt gtaaatggta caaagaaaaa
    31861 gtggaaaact gtagtcatcg caaattgaaa acgagctaaa gtttcaattg tcatacaaaa
    31921 cctcttattt tgttttaatc atttctttta tagaaatatc tgcaagagct agtttcctag
    31981 cttcgtaaca attggtaaat aacttacttt aatatcttaa tgatataaat caaaacgtcc
    32041 cttagctaga agttctttaa gtccacccat atcaacaagt gatttgttca tgataatctt
    32101 cttcataacc gacgcagggt attttttaat tttagttttt ccaacaacac caagtccatg
    32161 cgtatttcct actgaagcaa ctgtgccttg cggactatac gaaaagtctt ctgtagcttc
    32221 acctttgatt tgatgtaaaa ggtttttagc aacatgagct cccatacgag ttgcaatttg
    32281 agctgttgtt gggaatgggc gaccagactc agtgtccata aatgcagaca catcaccgat
    32341 aacataaaca ttatcgtatt ttggatcacg caaatcactg ttgaccatta cacgtccacg
    32401 acgttgatca aatcctgatt ctcccataac cggacttcca ctaacacctg ttgtccaaat
    32461 gatagtacct gcggtaatag atttgagttc ttcatcctct ttactagtac cataaacaac
    32521 ttcacccggt ttgatttcct taatcatact tccaagcatc aaattaatac ctaaatcctt
    32581 aattaagttg acaccatatt gcgctaattc atcatcaaac attggtagga tgcgagttgc
    32641 tgcctctaca caaataattt caatttgttc aggttttaca cccgctaaag cagcatattt
    32701 tggtcgctca tcaaccatag ctccggctaa ctcaatacct gtaaaaccag caccacatac
    32761 taagagtttg agaagatttt catcttttgt ctcacggtac aacttcattg tattaacaat
    32821 gtgattatgg atgttttctg cttgcgaaat attggtcatc tgcaaagcat tctccatagc
    32881 acccttgatg ccaaaagttt cagatacaaa gcctaaggca actacgacat aatcataatg
    32941 gagttcaccg ttatttttaa ctgttactgt tttattttca gcatcgactc taagcacctc
    33001 atcttgcatg aagttaacct tattagtgtt gataacatcc ttaattggga atgtaatttt
    33061 ttcttttggc tgagagcctg cagctacttc atgtaattct gtagcttcgt agtgataatc
    33121 attcatgtca acaagggtaa tatgaaaatc acctgattgt ttttgtaaat tacgaactgc
    33181 tttcaaaccg gcataaccag cacctaaaac taggatttct ttcatgataa aactcctcta
    33241 aaaaatgatt ccaattagat acgttaagac ttgggttgca gatcccagtg ctagaatacg
    33301 taccgcacaa acaaatgttt ctctcttaac ctgtttttgc cacaatagtt ttgtctgctt
    33361 ataaataaat ggtattaata acaaactcaa gagtactgta acaggcgcaa gtccaaaaag
    33421 aaattcaaaa acaattgcca ataaagctat actattagaa atagcaaaca gcaagagtcc
    33481 acctcttatc cctgtataat gtacaagggt atagcgatga tttttttcat cttcttcttt
    33541 gtcgcacaga ttattcgcca acattaggtt ggcaatccat aaggtgttcg gcaaggaaat
    33601 caagaaaatt ttgcttaagt tgaggatatc ccacgaaaag ttttgataag tgtttagata
    33661 tacacaaatt aaactaatca taaatcccat tgtcaagcca gaaaatactt ctcctaaggg
    33721 caaactagat aaaggtcttg gaccaaagga atataaaaca ccaattccaa aacaaaaaag
    33781 tcccatccat aaaagaggca gaccgacttg ggaaactaaa actaaaccaa tcatactaga
    33841 agtaatgact aaacttgcca taataacttc tatttggcgt agggataggt tctcacgacc
    33901 aataatgtta gtgtcattct tataatccaa atccacagca ttccgataat cattgtaatt
    33961 attccaaatg tcaacgaaca tattgaataa aaacatagca acgaagaata aaccgacata
    34021 tacagggtgt actgaattgt agtaataagc actaaaacac aaaccaatca aaaatggtaa
    34081 aacactagct gttttggctt tcatttctac caactctaga aagattggta atgtcattat
    34141 taaagtcctc tctatacata atcttcaaca atactattat aatgcaattt ctaccatttt
    34201 taaaacaata gggcttgatt tttgattaaa aatacaaaaa atttttctta aaatgagtta
    34261 atcacactta gaacactaaa tactattttg aggagtccta ttcatcacac tatgccatgt
    34321 aatattcgga aaaatagtat tgcaaccctc aactatccct tatgttggca ccagcctttg
    34381 aaagtcctct gattgcactg cttagttagt attttcatca gctgtatgat ctctaagagc
    34441 catcagcata ctaaaaaaac tagtattaca ctttgaaaag tgtaacacta gctccaattt
    34501 gttttaaata gcttaactat ttaatcatta atctacttta tatatttaag catttaaagt
    34561 tgaatgttgg ttgaggatac ctgagagaga accttgaata taatttggca atacttgttt
    34621 ttgagcacgt tcatagattt ctgaatctgt taactgacgt tctgatttgg ggcttgatac
    34681 ttgcggtgtc actgctgcat tttgttgttt gtaaagtgtt gaatgctggt taaggatacc
    34741 tgagagagaa ccttggatat attttggtaa tacttgtttt tgggcacttt cataaagttc
    34801 tgaatctgtt aactgacgtt ctgatttggg gcttgatact tgcggtgtca ctgctgcatt
    34861 ttgttgtttg taaagtgttg aatgctggtt aaggatacct gagagagaac cttggatata
    34921 tcttggcaat acttgttttt gggcacgttc ataaagttct gaatctgtta actgacgttc
    34981 tgcttttgga ggtgttgctt gcggtgctgt gaaagttgaa gctgaaacac cttgtgtaga
    35041 accaagagct acgaatgctc ctccagcaaa cattgctgag acaagaccaa gtttcaaata
    35101 gttagcagtt gagacgttgt tgactttttt ttctgtaata agtttgttca taagaattac
    35161 ctctttcttt tgggttattt attttatgaa ctaagtataa caacaagaaa taaagtaagg
    35221 ttaaactaaa tataaaataa ttcttaagtc attcttagga aaccataaaa tcatacacta
    35281 acaaaaaatt tggtatctag ctcaaaagta aaagtagaat aaaggctttt acttctgagc
    35341 tagtaatgaa taaattatat ttgcatctag tttggaatta gccctagtgt attataatga
    35401 tccctacaaa tatgtgccaa acaccttctt catatataca agattgattt aacattccta
    35461 tacccttaat ggtgggcaca ttctcttcat gttccatata gaaatcaaca gataattccg
    35521 tattgtccct attattaaat tttccagtta atgataaatt atatcgtcca ccttgaggtg
    35581 tcctattacc aatctgttca acctttacac ttgacgaatc gaattgaaca gattctgtct
    35641 tatcattctg tgatttagaa catcaacaat ttcttgttca tgttctttga ggtaagctag
    35701 ttgttcttct ttaacgtgct tttatctttc gtacttactg atacttgaga gcattttttt
    35761 gaccctactt cttactaatc agctgacgtc cctataagat gaatcataaa cacatagctg
    35821 ccactaacca ttttaacctt ttatgcatac ataattcttc ttgatatccc ataccattat
    35881 ctttagtata aacacttaat aaaacaagca tttaacaact aaggttaata aaattcaata
    35941 aatctaatcc ttctacttac tttagtttat cacttttttc tctcatccta tgctataata
    36001 gtaaggtatc aaaatttagg agaatatagt cttatgatga atatgcaaaa tatgatgcgt
    36061 caagcacaaa agcttcaaaa acaaatggaa caaacgcaag ctgatttagc agctagccag
    36121 tttactggaa aatctgctca agagctcgtt accgtgacat ttacaggcga taaaaagcta
    36181 atttcaattg attacaaaga agctgtggta gaccctgaag atattgaaac actacaagac
    36241 atgacaactc aagctatcaa tgacgcttta agccaagttg acgatgctac aaaaaaaatt
    36301 atgggagcat ttgcgggtaa aatgccattc taaaaaataa atcacattta ctgaatcacc
    36361 tgtaaatgtg atttttattt caaacttctt tcattttttc acgaaaacaa gttttatttt
    36421 caaaaaatat ttgcaaaaaa tcagcagaga ggtataataa cattgaaaat attagagaga
    36481 aggtgactaa ccatggttat ggatagtcta atcataaaac agttgattaa gagtacattt
    36541 gatataccct tacaagtcac ttatccaaat gggaatattg aaacttataa tggtagcaat
    36601 ccccacgtca aacttaaact aaacaaaaat ttctccgtct cggagctatc aaaagaccct
    36661 tccatagtat tgggtgaagc tgttatggat ggagacattg aaatttatgg ttctatacaa
    36721 gaattaattt tatcagctta ccgctgtgga gacagctttc tacgtaatag taaatttagc
    36781 aaattgattc ctaaacaatt tcatgataaa aaacacagta aatccgacat tcaaaagcat
    36841 tatgacatcg gaaatgattt ctataaactg tggcttgatg ataccatgac ttattcatgc
    36901 gcctatttta aacatgaaaa tgatagtcta gagcaagctc aactaaacaa agtacaccat
    36961 attcttaata aattaaatgc tcagccggga gggaaattac tagacatcgg ttgtggctgg
    37021 gggacactta tcattactgc tgctaaagaa tatggcttaa atgcgacagg tatcacctta
    37081 agtgaagagc aggcttcttt cattactaag cgtatcaagg aagaagggtt ggaaaataaa
    37141 gtaacagtcc tcattaaaga ttatcgtgac attcgtgaaa catacgatta tatcactagt
    37201 gttggtatgt ttgaacatgt aggaaaagaa aatttatctc aatacttcca aaccatttct
    37261 aaaagactca acatcaatgg tctagctctt attcatggta ttactggaca agtgggtggt
    37321 aatcatggta gtggaactaa ttcttggatt aataagtata tcttcccagg aggatatatc
    37381 cctcgtttaa ctgaaaattt aaatcatata gctagtgctg gacttcaaat tgctgactta
    37441 gaaccgcttc gtcgtcacta tcaaaagact ctcgaattat ggacaaaaaa tttccacaat
    37501 gcccttcctg aagttcagaa aactcatgat aagcgtttta tcaatatgtg ggacctctat
    37561 cttcaatctt gtgcggcctc ttttgaatct ggcaatattg atatttttca atacttgctt
    37621 tcaaaaggtg tttcaaaaga cactatgcct atgacacgcg attatatgta ttctgctaat
    37681 tgatatagtt aatattatag tattccaact tattctaaat aaaaaggact ctcttgaatt
    37741 caagggagtt ctttaacgtt cttctctggc taactctata caatgtgact tttggtgaca
    37801 atgtggacag aatacttttc gcgtcttagg ggtatgtttc gctagattaa atgcttgata
    37861 tgtcggtgta aaggtttgat gacaatttgg acaaagataa acaacctgtt gtttaaaata
    37921 ccaaaccata ccattcatag ctattaagaa tactgttact gctatccaca taagccatct
    37981 ttgatttaga gaaactgctg ctattattaa cgtaatatag accataataa tcataacgac
    38041 ttgtagcact atgctttttt gtagtatacc ccattttctt ttgttttctt ccataagcac
    38101 aatgtcagaa agatagccta atgttttact atcttgtttt tctacagttt ttagtaggtt
    38161 gacagctttg tcaagtttgt cttttttact atcaatagct agccttaatt ctcttatcta
    38221 cacttgtaaa aaaagctcta aaatctggct agcattgtct tcagtaaata gatttttgat
    38281 attatcaatt gaaaaatcta aatctcgtaa ataaataatt tgtctaagct tttctaaatc
    38341 agcaatacta taaaccctcc tacctccttc agatagttct gatggggata aaattcctct
    38401 tttgtcatag tactgaacag tacgaacagt aactcctgct tctttagcta agtcaccagt
    38461 ggaatatttt tctgacatgt ttcacacctc ctttaatata gtaaacactt ctttatcaga
    38521 aacaccttta ctatatcaaa tgacctaagg taataagcaa gtaataacgc taagtatttt
    38581 taactttaaa taaaactaca acaattaatc aaacaaattt cctaaagtcg caaatgggct
    38641 atcaactgct acctcttttt gctgttttaa atagatttta ttttcatcca agtccagaaa
    38701 tttttcataa acacgcgcag tcatacgggc atcctctaag ctattatgtg ctttaccctt
    38761 tattcctaaa aagtcagcaa cagtagtcaa tttaaggttg acaataccat ttaaatctgt
    38821 actgcgtctc acaaaagctt catcatataa gtccacctga tattgactag ttagatccaa
    38881 accattttca actaaaaggg gtaaatccga tttataccca ttataaccaa tcagtggtgt
    38941 atctccaaca aaactctgaa agtctgtcaa aactatctca attttaggag cccctataat
    39001 atctctagct gtaatacctg ttaaaccatt aataaagctt tgtaatggaa cttttgtgtg
    39061 aacataagta tcaaataaag caatctcttg atgattagaa tatttaacgg ccgacacttg
    39121 aataatatgg ctatgctcac caaccgtgtt aaactctaaa tcaaaagcta tatattcttg
    39181 taattgtttc attatttctc ctaaaaagcg caaggaaact ccttacgctt ttattctatt
    39241 ttttattgaa gaaacgtcca aagaaacttt ttttaacagc ttctaattct tgaatttttt
    39301 cttctttggc ttcttgaatc tcctcaacct ctgctttagc ttcatttaac tcaagttgca
    39361 gacgttgcgt atcttccata gcagttaaag tcaattgctg ttgttgatct aattgcttat
    39421 ctttctctgc tatttgaata tcttttacat gtaactgctc gtcttttgaa gcaatctgaa
    39481 tatcttttgc ttttaactgt tcatataaac gtgtgatttc agtattcttc tcatcaacga
    39541 gaatttctaa aagttctcgt cttgatgctt cttcgtcaat tggttcctct tccaaaatag
    39601 tttttttata gatttcttcc aatttaatca agccatcacg attaacaact gtaacgcctt
    39661 tactattctt ttccacacat tcttcaggga gtgttttaac tctgttattc atcgcttgac
    39721 gactaacccc taaaatttca gctaattcac taaccgtctt ttcaactgtc ataacttcct
    39781 cttttgtact gattttctta taaaagaata tcacaatcct aatctagtgt caatttactg
    39841 tcaacttaaa gtgtcaaaca agacattttg acgtctattt ctctatttat atgctagaat
    39901 aagattatga agcattatga cacaataatt ataggtggtg gacctgctgg aatgatggca
    39961 gctatttcct caaattttta cggcaacaaa acacttctta ttgagaaaaa taaacgttta
    40021 ggcaaaaaac tagctggaac aggtggtggc cgctgtaatg ttaccaataa tggaaacctt
    40081 gatgagctct tagctggtat acctggaaat ggccgtttcc tatatagcgt cttctcacaa
    40141 tttgataacc atgatattat taacttcttt caagacaacg gcgtcacact taaagaagaa
    40201 gatcatgggc gtatgttccc tactactgat aaatctcgta ctatcattaa cgcccttgaa
    40261 aataaaataa aagaacttgg tggtcaaatt atgactgaca cagaagttgt ctctgtcaaa
    40321 aagataggag attcattcta tatcaaaact aaagatacac agttcgcatc cgataaactt
    40381 atcgtaacaa ctggtggtaa atcctatccg tctacaggtt ctactggttt cggtcatgac
    40441 attgctcgtc actttaaatt agaagtcaca gatatggaag ccgccgaaag ccctctttta
    40501 actgattttc cccataaaaa actacaaggt atctccttag atgatgtcac tttgtcattt
    40561 gaaaagcata ttattacaca cgatcttctc tttactcatt ttggattatc aggaccagca
    40621 gccttgcgaa tttctagctt tgtcaaaggc ggagaaacta tatacctaga tgttttgccc
    40681 aatatctctg ttaaagaatt ggaaatacac ttccaaaatg aaagagaaaa atctttgaaa
    40741 aacgctctta aaatacttct tcctgagcgt ttagctgaat tttacgctga agacttaccc
    40801 gaaaaagtaa aacaagtatc tgtaaaagac ttagaaatgt taattcaaaa acttaaaaaa
    40861 cttcctattt tagtaactgg caagatgtct ctagcaaaat cctttgtaac aaaaggggga
    40921 gttgatctta aagaaataaa ccccaaaaca cttgaaagta aaaaggtagc tggtttacat
    40981 tttgctggtg aagtcctaga catcaatgct catacgggag ggtttaacat tacttctgcc
    41041 ttatgtacag gctgggtagc tggtagctta cactattaat acatgaaagc tgaacaattc
    41101 cgtcagcttt tttatataga atttaaagat aatatttggc tcttaaggct tctttgattt
    41161 tttccaattc atctggtaaa tttacctgat gaatagcata cccaatctga taaggggata
    41221 ctataaaacg aggaacaatt ttacaaataa gacccccatc atgcttatag aaaaagagat
    41281 tataagattt acagcgccct cccaagaatt catttaaaat ataatgacac aaaattggta
    41341 aactatctgc aatcaaaata gcgttttcca atttggatac tttaattgtg aactctgtga
    41401 aatcaagcat aggacgattg gcaatactaa tctctgcttg ttgactcgtt tccgaacaaa
    41461 cctcgatacc atcaaaccaa tctaagttca acgtttcata agcatctacg ttttttaagc
    41521 caacaatttg gctatgagga tgtcgtaaac ttccgccaga taagggccca aaatttcgat
    41581 aaagaacgac cgaacggtaa tgaccacttt cttccattaa ttgccatttt gacaaaagat
    41641 aatctaacaa ttggcgcatc tttgactcac tataaactga aatatcccca ttgtgatcat
    41701 ttgattcaat caccaatgtt tgataggttt cttctaaagt tgggtattta ttttctaccc
    41761 agatcatatc gtctttcacg tctaggattt ttcctaattg gcttttatca caaaatggac
    41821 atttgtcttt attgctgggt ttttgtttcg cgatattagg gttaaatcgg atatatcttt
    41881 cattcatact gttattgtaa cacattcatc accattacaa tataatactt atccagttaa
    41941 cattttcctt gcattcttag ctgaaaagag tataatagaa tcatgtcttg tttaccggtt
    42001 aattaccagt taactaggca ccctgttttg ggatagaaaa atctttttgg gaaaggagaa
    42061 atcatggcta agaaatcaaa aattgctaaa tttcaaaaac agcaaaaact cgtagaacaa
    42121 tatgctgaac tacgtcgtga gttaaaagaa aagggtgatt acgaagcact tcgtaaactt
    42181 cctaaagatt ctaaccctaa ccgacttaaa aatcgtgatt taatcgatgg ccgtcctcac
    42241 gcttacatgc gtaaattcgg tatgagtcgt attaacttcc gtaacctagc ttacaaaggc
    42301 caaattcctg gcattaaaaa agcaagctgg taattcgtga agacaaatag ttacttttta
    42361 tgactattct attgagtgtg gacgaatagt taatacattg taattattta acctgttgag
    42421 tatttactca gctattacaa acttcttc
//
LOCUS       DATROQ010000008        41841 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.008, whole genome shotgun sequence.
ACCESSION   DATROQ010000008 DATROQ010000000
VERSION     DATROQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41841
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.008"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(102..1121)
                     /locus_tag="VBC94_000374"
     CDS             complement(102..1121)
                     /locus_tag="VBC94_000374"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO6978674.1"
                     /translation="MYLIEPIRNGKRITDGAIALAMQVYILQNVFLDDDILFPYYCDP
                     KVEIGKFQNAVIETNQEYLKEHDIPVVRRDTGGGAVYVDSGAVNICYLMKDHGQFGDF
                     KRAYEPAIKALKTLGASSVEMRGRNDLVIDGKKVSGAAMTIVNGRIYGGYSLLLDVDF
                     DAMEKVLNPNRKKIESKGIKSVRSRVGDIRSHLSEDYRHITTDQFKDLMVCQLLHIDH
                     IDQAKRYHLTEKDWAAIDALADEKYKNWDWNYGNSPQYSYHRDTRFPSGTYDFHLEIE
                     KGIITNCRIYGDFFSSKDISDIENLLIGCPMKEELVLEKLSTLSLEDYFGQTSPEEIK
                     AVLFS"
     gene            complement(1148..2026)
                     /locus_tag="VBC94_000375"
     CDS             complement(1148..2026)
                     /locus_tag="VBC94_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEO6978675.1"
                     /translation="MTVWKTLEKTNHSQSEILSQLIEESDAIVVGIGAGMSAADGFTY
                     IGPRFEEAFPDFIAKYQLLDMLQASLYDFEDWEEYWAFQSRFVALNYLDQPVGQAYLD
                     LKDILAKKEYHIITTNADNAFAVADYNLEKVFHIQGEYGLWQCSQHCHQQTYRNDQAI
                     RQMIAQQKDMKIPSNLIPKCPKCDQPFEINKRNEEKGMVEDADFHAQRQRYENFLSQH
                     QNDKVLYLEIGVGHTTPQFIKHPFWRFVSLNENSLFVTLNHKHYRIPQKIRSRSVQLT
                     QHIAELIAEAKKEIIG"
     gene            complement(1995..2813)
                     /locus_tag="VBC94_000376"
     CDS             complement(1995..2813)
                     /locus_tag="VBC94_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEO6978676.1"
                     /translation="MPNQKQLLLAMIEYLQSEKLTDVDDLRTTDLQTVWRGLVNQQDP
                     QNISQEYLSLEDRYLSHWWNTQKVKTIDVCHQTVYSNVFTYHGDICLLQVDAIVNAAN
                     SKLLGCFIPNHHCIDNQIHTFAGSRLRLACHQLMTQQGRMEAVGQAKLTESYHLPCKY
                     VIHTVGPYVKVDQKPSRIREDLLKSSYKSCLQLAAQANLKTIVFPCISTGEFGFPNQR
                     AAELAVQEILEWQRENQHKLYIIFNTFTPKDQEIYQKLLLKEEIDDSVEDSRKN"
     gene            complement(2806..3138)
                     /locus_tag="VBC94_000377"
     CDS             complement(2806..3138)
                     /locus_tag="VBC94_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEO6978677.1"
                     /translation="MKKIANYLLIEKNEELYTISLTPELQDDLGTVGYVEFTDDTNLE
                     VDDVILNIEASKTVMAILSPLTGKVVKVNTAASQEPTLLNSEKADENWLVVLTDVDYA
                     AFEALENA"
     gene            complement(3167..4153)
                     /locus_tag="VBC94_000378"
     CDS             complement(3167..4153)
                     /locus_tag="VBC94_000378"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HEO6978678.1"
                     /translation="MKLSVLDYGLIDYGKTASDAIQETILLSQEAEQLGYHQFWVAEH
                     HGVKAFSISNPELMIMHLANQTKSIKIGSGGIMPLHYSSFKLAETLKTLETCHPNRVS
                     IGLGNSLGTVKVSNALRSLHKAHDYEEVLEELKSWLIDESSSKEPLVQPTLSSFPDLY
                     VLGSGQKSAYLAAKLGLGFTFGVFPFMDKDPLTEAKKLSSLYYHQFEEYYPNKSPNLM
                     VAPFVVIADTSEEAENIAKTLDIWMLGNKDFNEFATFPTIEEANHYQLTPEQKAKIKS
                     NRHRMIVGDPKQVKESLDALVNASQAEELLLIPLVPGLDQRIKSLKLLSQLY"
     gene            complement(4150..5349)
                     /locus_tag="VBC94_000379"
     CDS             complement(4150..5349)
                     /locus_tag="VBC94_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEO6978679.1"
                     /translation="MNNVQGNLFRPLTLPNGLSLENRFVLSPMVTNSSTSEGFVTDDD
                     IAYAVRRAKSAPLQITGAAYITEYGQLFEYGFSVSKDEDIPGLTKLAKAMKSKGAKAV
                     LQLTHAGRFSSHTLARHGYVYGPSPMQLQSPYPHQVKELTHKDILRIIDEYVQATRRA
                     IQAGFDGVEISSAQRLLIQTFFSTFSNQRKDEYGPQTLTNRCRLGLEVFKAVQKVIRE
                     EAESDFILGFRATPEETRGSQIGYSIEEFMEFLEKILAIAQVDYLAIASWGHDVFRNT
                     IRSEGVYKGQLVNQVIFEHFGDRVPIMATGGINSASKVFEALQHAHMIGASTPLVVDP
                     EFLQKIKAKRSDQINLRIKVNDLEGLAIPKASFKDIVPLMDYGESLPKEAREVFRELR
                     SNYREER"
     gene            complement(5342..6178)
                     /locus_tag="VBC94_000380"
     CDS             complement(5342..6178)
                     /locus_tag="VBC94_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEO6978680.1"
                     /translation="MEWQDLAQLPVSIFKDYVTDAQDAEKPFIWTEVFLREINRSNQE
                     IILHIWPMTKTVILGMLDRELPHLELAKKEIISRGYEPVVRNFGGLAVVADEGILNFS
                     LVIPDVFERKLSISDGYLIMVDFIRSIFSDFYQPIEHFEVETSYCPGKFDLSINGKKF
                     AGLAQRRIKNGIAVSIYLSVCGDQKGRSQMISDFYKIGLGDTGSPIAYPNVDPEIMAN
                     LSDLLDCPMTVEDVIDRMLISLKQVGFNDRLLMIRPDLVAEFDRFQAKSMANKGMVSR
                     DE"
     gene            6317..7015
                     /gene="coaB"
                     /locus_tag="VBC94_000381"
     CDS             6317..7015
                     /gene="coaB"
                     /locus_tag="VBC94_000381"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEO6978681.1"
                     /translation="MYNMAMKILITSGGTTEKIDTVRSITNHATGALGKIIAEKYLRE
                     GHQVTLVTTKNAVKPESATNLSIFEIEDVDSLIKTLKPLVKEHDILIHSMAVSDYTPV
                     YMADFEKVKSSDDLDTFLRKDNHEGKISSESEYQVLFLKKTPKVISLVKKWNPQITLV
                     GFKLLVNVTKENLFKVARHSLIKNKATFILANDLIDITSKHHIAYLLDHDNVYKATTK
                     EDIAQLIYEKVKKI"
     gene            7012..7554
                     /gene="coaC"
                     /locus_tag="VBC94_000382"
     CDS             7012..7554
                     /gene="coaC"
                     /locus_tag="VBC94_000382"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEO6978682.1"
                     /translation="MIKRITLAVTGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNPIHLDVMDEHNPKIINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALAMPDETPKLIAPAMNTKMYHNTITQRNIDILKKIGYQEIEPRISLLACGDT
                     GQGALADISTILKCIQEVAN"
     gene            7600..8172
                     /locus_tag="VBC94_000383"
     CDS             7600..8172
                     /locus_tag="VBC94_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6978683.1"
                     /translation="MKKKNKSSNIAIIAIFFAIMLVIHFLSSFIFSFWLVPIKPTLMH
                     IPVIIASIAYGPRIGATLGALMGGISVANSSIVLLPTSYLFSPFVENGNFYSLIIALV
                     PRILIGIIPYFVYKLLHNRFGLAISGAIGSLTNTVFVLSGIFIFFSSTYNGNIKLMLA
                     GIISSNSLAEMVIAAIIVYLTVPRILNIKH"
     gene            8282..10000
                     /locus_tag="VBC94_000384"
     CDS             8282..10000
                     /locus_tag="VBC94_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO6978684.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSSGLIQVIGEDVDIEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVNADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQIATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATVGNDLADAETKIANIEKE
                     ITTFVN"
     gene            complement(10064..10261)
                     /locus_tag="VBC94_000385"
     CDS             complement(10064..10261)
                     /locus_tag="VBC94_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978685.1"
                     /translation="MKKRLQLLLLIFISAVSWFISNQLATKNTVLSVLIGLVAVIGIT
                     WLIVSLLYYLIVKLIAVIFHD"
     gene            complement(10275..12008)
                     /locus_tag="VBC94_000386"
     CDS             complement(10275..12008)
                     /locus_tag="VBC94_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6978686.1"
                     /translation="MKASHPKKIQKKLVQDLLSKKSLVGMALLGTVVQVCLTVYLPVL
                     IGQAVDVVLSPHSMILLLPIMWKMVAVILANTIIQWINPLLYNRLIFHYVASLRKAVM
                     EKLNLLPIAYLDKRGIGDLISRVTTDTEQLSNGLLMVFNQFFVGLLTILVTIFSMAKI
                     DLLMLFLVLFLTPLSLFLARFIAKKSYHLYQNQTASRGRQTQFIEEMVSQESLIQAFS
                     AQEESSDHFRTINQEYANFSQSAIFYSSTVNPSTRFINSLIYGFLAGIGALRIMSGAF
                     SVGQLITFLNYVNQYTKPFNDISSVLSEMQSALACAERLYSILEESSPNITGTEKLDS
                     STVKGQIDFKNVIFGYNKSKPLLNGINLHIPAGAKVAIVGPTGAGKSTLINLLMRFYE
                     VDGGNILLDGKPITDYEPSQLRQEIGMVLQETWLKSATIHDNIAYANPKASREEVIEA
                     AKAANADFFIKQLPNGYDTYLEDAGDSLSQGQCQLLTIARIFLKLPRILILDEATSSI
                     DTRTEVLVQEAFQMLMKGRTSFIIAHRLSTIQTADIILVMVSGEIVEVGNHSELIAQK
                     GIYYQMQNAQK"
     gene            complement(12009..13730)
                     /locus_tag="VBC94_000387"
     CDS             complement(12009..13730)
                     /locus_tag="VBC94_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6978687.1"
                     /translation="MKRLTYYFKGYIKETIFGPLFKLLEASFELLVPIVIAKMIDETI
                     PRGDRSSLLLQIGLIFFLAAVGVVVAITAQYYSSKAAVGYTRQLTEDLYQKVMSLGKK
                     DRDELGTASLITRLTADTFHIQTGLNQFLRLFLRAPIIVFGAIIMAFSISPSLTIWFL
                     IMVVTLFIIVFVMSRLLNPIYLKIRTSTDYLVKLTRQQLQGVRVIRAFNQVDRESEAF
                     NDINYHYTNLQLKAGRLSSLVTPLTFLVVNITLVVIIWRGNLNIANHLLSQGMLVALI
                     NYLLQILVELLKMTMLVTSLNQSYISAKRIIAVFERPSEIIDDKLEPKYSNKALEVQE
                     MAFSYPNSSEKALSDITFSMNVGETLGIIGGTGSGKSTLVNLLLHIYKVQEGDIDIYH
                     QGKSPDTISNWRSLVRVVPQNAQLFKGTIRSNLSLGLGKVSEEKLWTALEIAQASDFV
                     KEKDGQLDAPVESFGRNFSGGQRQRLTIARALVQDKIPFLILDDATSALDYLTEARLF
                     KAIIKHFNQTNLIIVSQRINSIQNADRILLLDKGKQVGFDNHQSLLAHNKVYKSIYHS
                     QNFKEEE"
     gene            complement(13742..14344)
                     /locus_tag="VBC94_000388"
     CDS             complement(13742..14344)
                     /locus_tag="VBC94_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEO6978688.1"
                     /translation="MFKFLKRLIALIIIIFIGYRLVIIHENVKKVLQYHDLVQNTLAE
                     NGSEANVHLVLSMIYTETKGDAIDVMQSSESISGTTNSITDSHTSIKHGVTLLSQNIS
                     QAKKAKVDVWTAVQAYNFGSSYIDYVADHGGENSIELAKNYSKNVVAPSLGNYNGDTY
                     FYYHPLALISGGKLYKNGGNIYYSREVQFNLYLIKIMELF"
     gene            complement(14346..15323)
                     /locus_tag="VBC94_000389"
     CDS             complement(14346..15323)
                     /locus_tag="VBC94_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEO6978689.1"
                     /translation="MMDFYIKQIIIHQFSPNDTELVLSDTPLTLTPRIDDYFRKKLSK
                     VFSDEAKRGYFGEDNVFMSHLQDDLYVSSCQIAQLWKEEFVISEDQKTNDLVFIQFDK
                     DGMEHFAFLRISLKEQFAHVSENQEQPITITQNNLPSAAQTPDEALVVNKSSKQYYLI
                     EKRIKHNGSFANYFSENLLQVQPEQSVKKSIKMVEQTAQKIAENFNKDDFSFQSKMKS
                     AIYKNLEEEQELSPEKLADQLFDDNLTARLTFVDELKDAIPGSVQVSDIDHSRQIKKL
                     ENQKLSLSNGIELIVPNNVYQDAESVEFIQNPDGTYSILIKNIQDIQNK"
     gene            complement(15328..16584)
                     /locus_tag="VBC94_000390"
     CDS             complement(15328..16584)
                     /locus_tag="VBC94_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEO6978690.1"
                     /translation="MIFDKDNFKEFDQELWQAIHDEEIRQQNNIELIASENVVSKAVM
                     AAQGSVLTNKYAEGYPSHRYYGGTDCVDVVESLAIERAKMLFNAEFANVQPHSGSQAN
                     AAAYMALIEPGDTVLGMDLAAGGHLTHGASVSFSGKTYHFVSYSVDPKTEMLDYDNIL
                     KIAQETQPKLIVAGASAYSRIIDFEKFRQIADAVDAYLMVDMAHIAGLVASGHHPSPI
                     PYAHVTTTTTHKTLRGPRGGLILTNDEAIAKKINSAVFPGLQGGPLEHVIAAKAVALK
                     EALDPSFKIYGEDIIKNAQAMAKVFKEDDDFHLISDGTDNHLFLVDVTKVIENGKKAQ
                     NVLEEVNITLNKNSIPFERLSPFKTSGIRIGTPAITSRGMGVEESRRIAELMIKALKN
                     HENQDVLTEVRQEIKSLTDAFPLYEN"
     gene            complement(16676..17272)
                     /locus_tag="VBC94_000391"
     CDS             complement(16676..17272)
                     /locus_tag="VBC94_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEO6978691.1"
                     /translation="MNDLGQILEDHGAVIMPTETVYGIFAKALSEEAVNHVYELKKRP
                     RDKAMNLNICDFETILKYSKNQPTYLKQLYDAFLPGPLTIILEASQEVPDWINSGLSS
                     VGFRMPKHPVTLDMIANHGPLIGPSANISGCDSGRVFSEIQKQFNHQVLGIEDDKALT
                     GVDSTIIDLSGDRVKILRQGAITQEVLTATIPELIFEN"
     gene            complement(17265..18095)
                     /gene="prmC"
                     /locus_tag="VBC94_000392"
     CDS             complement(17265..18095)
                     /gene="prmC"
                     /locus_tag="VBC94_000392"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEO6978692.1"
                     /translation="MNYAQLIKHYEQLLEACGEEVENFIYVLKDLKQWSTTDYLLNQN
                     SSVSDTDQQLMENIFQQLKKHRSPQYITGKAYFRDLIFFVDERVLIPRPETEELVDLI
                     LSENKVEDCSVLDIGTGSGAIAISLKKERPSWDVLASDISVSALDLAKENANNCDAEV
                     TFIESDVFSNISDKFDIIVSNPPYISYNDKDEVGKNVLASEPHSALFADEEGLAIYRK
                     IIENSREYLQPRGKLYFEIGYKQGDDLRSLLKRYFPNNRCRVLKDIFGKDRMVVLDNE
                     "
     gene            complement(18095..19174)
                     /gene="prfA"
                     /locus_tag="VBC94_000393"
     CDS             complement(18095..19174)
                     /gene="prfA"
                     /locus_tag="VBC94_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEO6978693.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQNISDAEEMIKDASGDAELEEMAKEELKESKAAKEEYEERLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYEI
                     DQKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTQKLEALNK"
     gene            complement(19209..19778)
                     /locus_tag="VBC94_000394"
     CDS             complement(19209..19778)
                     /locus_tag="VBC94_000394"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEO6978694.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     EFGVVSSRIGMRREAVPISDDMDIFSYIQNLPQKPYCVLIDECQFLSKKNVYDLARVV
                     DDLDVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPDI"
     gene            19916..20098
                     /locus_tag="VBC94_000395"
     CDS             19916..20098
                     /locus_tag="VBC94_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEO6978695.1"
                     /translation="MPFVKIDLFEGRSQEQKNELAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGELKKK"
     gene            20247..21185
                     /locus_tag="VBC94_000396"
     CDS             20247..21185
                     /locus_tag="VBC94_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEO6978696.1"
                     /translation="MQLSHSLRLMGTTIDIQINSKNAQKQIQEVIELLELYKNRFSAN
                     DFNSELMAINNNAGIKPIQVHPDLFELITIGKEHSLARPSNLNIAIGPLVQTWRIGFS
                     DAKLPSPSEVSEAMILSDPTHILLDSKNQSVFLNQIGMKIDLGALAKGYIADKIMTYL
                     KNEMIDSAIINLGGNVLVHGDNPNRSEGYWVIGIQHPKKKRGQNIGAVKIKNQSVVTS
                     GTYERRLIIDDKEYHHIFDRQTGYPIQTEMASISIVSKQSVDCEIWTTRLFGLSIKEA
                     LDILNAVSYIEGIIITKDDRIYLSDGLKHHFQLFYH"
     gene            21203..21805
                     /locus_tag="VBC94_000397"
     CDS             21203..21805
                     /locus_tag="VBC94_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6978697.1"
                     /translation="MKLIGIVGTNSNKSTNRQLLQYMQQHFADKAEIELIEVKDLPLF
                     NKPADKNVPQVILDIAAKIEEADGVIIGTPEYDHSIPSALMSVLAWLSYGIYPLLNKP
                     VMITGASYGTLGSSRAQLQLRQILNAPELKASVLPDEFLLSHSLQAFDKDGNLHDIET
                     SQKLDAIFDDFRLFVKITGKLSNARDLLQKEAENFDWESL"
     gene            21829..23064
                     /locus_tag="VBC94_000398"
     CDS             21829..23064
                     /locus_tag="VBC94_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6978698.1"
                     /translation="MKFVGIVGSNAEQSYNRMLLEFIRKNFKTKFELEVLEIDDIPMF
                     NQVQNWEESFQLRLLNNKITRADGVIIATPEHNHTITAALKSVLEWLSFAVHPLENKP
                     VMIVGASYYDQGTSRAQIHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDDNGNIINPG
                     TVQFLETCLDNFIKYVGVVSSLRKPKAIESEDLYCQGQIDTTITSIDPDDPDWLDKAA
                     EKVNAVEGDAYVKLDNGLLTVNQLNMFLNAMPFELTYADDNNQFLYYNNNHPDPDTML
                     GKRVREQVGNRMATVHGSLPPARMKNVEWVIGTLRNGNQEYVRTVVPGTPPEVINTHN
                     YQAMYYPDGSYAGINEIIFNFKPWLDWYLQTTGQRLVGGTTAAPGASAVDATSSASTS
                     TEDAPVDQATDATSGASEH"
     gene            23163..23951
                     /locus_tag="VBC94_000399"
     CDS             23163..23951
                     /locus_tag="VBC94_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEO6978699.1"
                     /translation="MSLFQEKIAYNCAKKEALYKESLGRYALRSMLAGAYLTMSTAAG
                     IVAADTIGKISPALSGFVFAFIFSFGLIYVLIFNGELATSNMLYLTAGAYNKNISWKK
                     AITILIYCTFFNLVGACILAWLFNQSYSFQHLTNDSFLGHVVAKKLGKPSSGAFLEGI
                     IANMFVNLAILAYMLLKEESAKMTVILSAIFMFVFLSNEHLIANFASFMLAAFSHIEH
                     IKGFTLLNVIRQWTLVFFGNWIGGGVFIGLAYAWLNKTKTSHHE"
     gene            complement(24050..25324)
                     /locus_tag="VBC94_000400"
     CDS             complement(24050..25324)
                     /locus_tag="VBC94_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEO6978700.1"
                     /translation="MKFNEQSNSQAALLGLQHLLAMYAGSILVPIMIASALGYNAKQL
                     TYLIATDIFMCGIATLLQLRLSKHFGVGLPVVLGCAFQSVAPLSIIGAQQGSGYMFGA
                     LIASGIYVVLVAGIFSKVANFFPPIVTGSVITTIGLTLIPVAMGNMGDNAKEPSLQSL
                     TLSLVTIGVVLLINIFAKGFLKSISILIGLISGTILAAFMGLVDASVVAEAPLVHIPK
                     PFYFGAPRFEFTSILMMCIIATVSMVESTGVYLALSDITNDKLDSKRLRNGYRSEGLA
                     VLLSGLFNTFPYTGFSQNVGLVQISGIRTRKPIYFTALFLVILGLLPKFGAMAQMIPS
                     PVLGGAMLVLFGMVALQGMKMLNQVDFEHNEHNFIIAAVSIAAGVGFNETNLFISLPN
                     TLQMFLTNGIVISTLTAVVLNIILNGLPKKLI"
     gene            complement(25324..25905)
                     /locus_tag="VBC94_000401"
     CDS             complement(25324..25905)
                     /locus_tag="VBC94_000401"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEO6978701.1"
                     /translation="MKLLEERILKDGDVLGENILKVDSFLTHQVNFELMQEIGKVFAD
                     KYKEAGITKVVTIEASGIAPAVYAAQALGVPMIFAKKAKNITMTEGILTAEVYSFTKQ
                     VTSQVSIVSRFLSNDDTVLIIDDFLANGQAAKGLLEIIGQAGAKVAGIGIVIEKSFQD
                     GRDLLEKTGVPVTSLARIKAFENGRVVFAEADA"
     regulatory      complement(26009..26104)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(26160..27143)
                     /gene="guaC"
                     /locus_tag="VBC94_000402"
     CDS             complement(26160..27143)
                     /gene="guaC"
                     /locus_tag="VBC94_000402"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEO6978702.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIISSRSQADTSVKLGNYTFKLPVI
                     PANMQTIIDEEVAETLACEGYFYIMHRFNEEARRPFIKRMHDKGLIASISVGVKDYEY
                     DFVTSLKEDAPEFITIDIAHGHSNSVIEMIQHIKQELPGTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCSKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHLESPGKLVEVEGQQFKEYYGSASEYQKGEHKNVEGKKIL
                     LPVKGRLEDTLTEMQQDLQSSISYAGGKELDSLRHVDYVIVKNSIWNGDSI"
     gene            complement(27347..28687)
                     /locus_tag="VBC94_000403"
     CDS             complement(27347..28687)
                     /locus_tag="VBC94_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6978703.1"
                     /translation="MTKKGWRIMPAIITTAILGFSGILIETSMNVTFPLLMKEFGVNP
                     AVIQWVTTGNLLAVAVTVPLSAFMIKNLSERQIFTLANVLFLFGVLIDSFAPNLAILL
                     VGRVLQGVGTGLALPLLFHIILTQIPMERRGLMMGVAAMVTLLAPAVGPTYGGVISGM
                     LGWKMIFMLLAPILIISTFIGLASIPKRQVRINDKLNFPAFVSLGIGLATLLLAIEKM
                     SIFYLLVAIVSFVIFYYLNKQLEFLNLNVFKDKDFSILLYGVLAFQMIPLALSFLLPN
                     LLQLVLHQTSTKAGLFMFPGAIAVVFLSPFAGYLLDKIGAFKPIMIGISLSLIGLIGT
                     AIFIPAKFVVVLLAFDILTKIGMGIGASNMVTTALTKLKPAQSADGNSILNTLQQFAG
                     AFATAVASQIFTIGQVAIPKNGAIIGSQFAVLFVIVVVILAIVGLTYLRKRKAI"
     gene            28979..29650
                     /locus_tag="VBC94_000404"
     CDS             28979..29650
                     /locus_tag="VBC94_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978704.1"
                     /translation="MAFASNESENNFFITLKTYFNYLFSIQIITDISTLNHADFDGSF
                     AFHDIETSIPHLVIDSNYLAISQTNSKTEANDIKTFSELSKTMTEFYYMLNFDLFNHL
                     PYRFRLHNKDGQTIYSNHKPEDPFDIYPEEEYPIDKWVQNSLIEKKAKELHLLLPGAS
                     QDYILVQSYKRLENDSGQLVGYIEHVHNIKPLLEGYLKESGQAIVGWSDVTSGASISN
                     DDFEL"
     gene            complement(29721..31721)
                     /locus_tag="VBC94_000405"
     CDS             complement(29721..31721)
                     /locus_tag="VBC94_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEO6978705.1"
                     /translation="MQHVNHSSFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GISSVTESFILGSISLIIWTLTLITTIKYVLVALKADNHHEGGIFSLYTLVRKMTPWL
                     IVPAVIGGATLLSDGALTPAVTVTSAVEGLKVVPSLQHIFQNQSNVIFATLFILLLLF
                     AIQRFGTGVIGKLFGPIMFIWFAFLGISGLLNSFAHPEVFKAINPYYGLKLLFSPENH
                     KGIFILGSIFLATTGAEALYSDLGHVGRGNIHVSWPFVKVAIILSYCGQGAWILANKN
                     AGNELNPFFASIPSQFTMHVVILATLAAIIASQALISGSFTLVSEAMRLKIFPQFRST
                     YPGDNIGQTYIPVINWFLFAITTSIVLLFKTSAHMEAAYGLAITITMLMTTILLSFFL
                     IQKGVKRGLVLLMMIFFGILEGIFFLASAVKFMHGGYVVVIIAVAIIFIMTIWYKGSK
                     IVSRYVKLLDLKDYIGQLDKLRHDHRYPIYHTNVVYLTNRMEGDMIDKSIMYSILDKR
                     PKKAQVYWFVNIKVTDEPYTAEYKVDMMGTDFIVKVELYLGFKMRQTVSRYLRTIVEE
                     LLESGRLPKQGKTYSVRPDSKVGDFRFIVLDERFSSSQNLKPGERFVMLMKSSVKHWT
                     ATPIRWFGLQFSEVTTEVVPLIFTANRGLPIKEKIELTTTGD"
     gene            complement(31856..32616)
                     /locus_tag="VBC94_000406"
                     /pseudo
     CDS             complement(31856..32616)
                     /locus_tag="VBC94_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649058.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(32678..33670)
                     /gene="pta"
                     /locus_tag="VBC94_000407"
     CDS             complement(32678..33670)
                     /gene="pta"
                     /locus_tag="VBC94_000407"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEO6978706.1"
                     /translation="MRSLFGGLREKVASDYVKIVFPEGSDERVLRAAARLKFEGLVDP
                     VILGKADEVHDSLARLGFVDQDYSVIDPEQYEKFEEMKEAFVEIRKGKATMEDADRLL
                     KDVNYFGVMLVKLGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRENT
                     QERYIFADCAINIDPNAQELAEIAVNTADTAKIFDINPKIAMLSFSTKGSAKAPQAEK
                     VQEAAKIAKDLSPELAVDGELQFDAAFVPETAEIKAPNSDVAGKANVFIFPDLQSGNI
                     GYKIAQRLGMFEAIGPILQGLNAPVNDLSRGSSAEDIYKLAIITAAQAIDEKSK"
     gene            complement(33696..34580)
                     /locus_tag="VBC94_000408"
     CDS             complement(33696..34580)
                     /locus_tag="VBC94_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6978707.1"
                     /translation="MKFEYIAEERCKVKTLLKSHDVSRGLLAKIKYRGGKIFVNGEEQ
                     NAIFLLEIGDVVTIDIPDEPSHETLEPVPHDLDIVYEDDHFLILNKPFGFASIPSSIH
                     SNTIANFIKHYYVSNNYANQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQAKAIE
                     KRYYALVSGSGDLADSGDIIAPIARDVDSIITRRVHESGKYAHTSYQVVARYGDVRLV
                     DIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRMDLGINRQALHCHSLSFYDPFMGKIN
                     KQTLDLTDDFDSVIMELH"
     gene            complement(34577..35413)
                     /locus_tag="VBC94_000409"
     CDS             complement(34577..35413)
                     /locus_tag="VBC94_000409"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEO6978708.1"
                     /translation="MTQMNFTDRATRVAIIANGKYQSKRVASKLFAAFKHDPDFYLSK
                     KDPDIVISIGGDGMLLSAFHMYEKQLDKVRFVGVHTGHLGFYTDYRDFEVDTLINNLK
                     NDKGEQISYPILKVTITLEDGRVIRARALNESTIKRIEKTMVADVVINQVVFERFRGD
                     GILVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVYRTLGSSVIIPKKDAIE
                     IVPKRVGVYTISIDNKTVHYKNVTKIEYSIDEKSINFVSTPSHTSFWERVNDAFIGEP
                     EH"
     gene            complement(35388..36059)
                     /locus_tag="VBC94_000410"
     CDS             complement(35388..36059)
                     /locus_tag="VBC94_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO6978709.1"
                     /translation="MSMDWETFLDPYIQTVGELKIKLRGIRKQFRKQNRHSPIEFVTG
                     RVKSVESIQEKMVLRGISEENLAQDLQDIAGLRIMVQFVDDVDEVLALLRKRHDMTVV
                     QERDYITHMKSSGYRSYHVVVEYPVDTIDGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPEEIKQRLEKTAKIALELDEEMRKIREDIREAQLLFDPLNRKLSDGVGNSDDT
                     DEFYR"
     gene            36169..36741
                     /locus_tag="VBC94_000411"
     CDS             36169..36741
                     /locus_tag="VBC94_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEO6978710.1"
                     /translation="MTHLEIEYKTLLNKDEFNRLTSLFSHVQPITQTNYYFDTETFEM
                     KAHRMSLRIRTLPNRAELTLKIPREVGNLEHNHDLTLEEAKYIVKNGQFPEDTEIASL
                     ILEKGVDPTKLAVFGQLTTTRREMETSIGLMALDSNIYADIKDYELELEVKQPKQGKR
                     DFDQFLKENNINFKYAKSKVARFSATLKNN"
     gene            36918..37892
                     /locus_tag="VBC94_000412"
     CDS             36918..37892
                     /locus_tag="VBC94_000412"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO6978711.1"
                     /translation="MAEQYADKQIKLFSLTANREIAEKISQASGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVVPYFGYSR
                     QDRIAASREPITAKLVANMLVKAGVDRVLTLDLHAVQVQGFFDIPVDNLFTVPLFAEH
                     YNQLGLSGEDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREEGYIIGEVEG
                     KKAIIIDDILNTGKTFAEAAKILERGGATEIYAVASHGLFAGGAADILESAPIREIIV
                     TDSVLSKERIPSNIKYLTASHLIADAIIRIHERKPLSPLFSYRSDKKD"
     gene            37896..39011
                     /locus_tag="VBC94_000413"
     CDS             37896..39011
                     /locus_tag="VBC94_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6978712.1"
                     /translation="MIYLDNAATTALTPSVIEKMTNVMTSNYGNPSSIHTFGRQANQL
                     LRECRQIIAEYLNVNSREIIFTSGGTESNNTAIKGYALANQLKGKHIITSEIEHHSVL
                     HTMTYLSERFGFDITYLKPNHGQITAKDVQEALRDDTIMVSLMFANNETGDFLPIQEI
                     GQLLRNHQAVFHVDAVQVFSKMELDPHSLGIDFLAASAHKFHGPKGVGILYCAPHHFD
                     SLLHGGDQEEKRRASTENIIGIAGMSQALTDATTNTLKNWTHISQLRTTFLDAISDLD
                     FYLNNGQDCLPHVLNIGFPRQNNGLLLTQLDLAGFAVSTGSACTAGTVEPSHVLTSLY
                     GANSPRLNESIRISFSELNTQEEILELAKTLRKIIGD"
     gene            39013..39360
                     /locus_tag="VBC94_000414"
     CDS             39013..39360
                     /locus_tag="VBC94_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEO6978713.1"
                     /translation="MAFEKEINLPDCNYIYTISPNIKKYTLRDTTFTQNRLGNYELER
                     LLETVPNSGDGFPLKITINKELSGFKLLITDKSGLRIVNIFKSTDHKILQDKFYFLMD
                     SLVERNIFTKTAK"
     gene            39515..40153
                     /locus_tag="VBC94_000415"
     CDS             39515..40153
                     /locus_tag="VBC94_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEO6978714.1"
                     /translation="MIMDKSIPKATAKRLSLYYRIFKRFNTDGIEKASSKQIADALGI
                     DSATVRRDFSYFGELGRRGFGYDVKKLMNFFAEILNDHSTTNVMLVGCGNIGRALLHY
                     RFHDRNKMQISMAFDLDSNDLVGKTTEDGIPVYGISTINDHLIDSDIETAILTVPSTE
                     AQEVADILVKAGIKGILSFSPVHLTLPKDIIVQYVDLTSELQTLLYFMNQQR"
     gene            complement(40166..40846)
                     /gene="radC"
                     /locus_tag="VBC94_000416"
     CDS             complement(40166..40846)
                     /gene="radC"
                     /locus_tag="VBC94_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEO6978715.1"
                     /translation="MYHIELKKEALLPRERLVDLGADRLSNQELLAILLRTGIKEKPV
                     LEISTQILENISSLADFGQLSLQELQSIKGIGQVKSVEIKAMLELAKRIHKAEYDRKE
                     QILSSEQLARKMMLELGDKKQEHLVAIYMDTQNRIIEQRTIFIGTVRRSVAEPREILH
                     YACKNMATSLIIIHNHPSGSPNPSESDLSFTKKIKRSCDHLGIVCLDHIIVGKNKYYS
                     FREEADIL"
     gene            complement(40942..>41841)
                     /locus_tag="VBC94_000417"
     CDS             complement(40942..>41841)
                     /locus_tag="VBC94_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO6978716.1"
                     /translation="SVSMILSYATFIGLIVWLFSIVIPDLISSLSSLLVIDTGALAKL
                     VNNLNENKQISEALNYMGTDKDLVSTLSGYSQQILKQVLSVLTNLLTSVSSIAATLLN
                     VFVSFIFSIYVLANKEQLGRQFNLLIDTYLGSTGKTFHYVRHILHQRFHGFFVSQTLE
                     AMILGSLTVIGMLIFQFPYALTVGVLVAFTALIPVVGAYIGVTIGFILIATESLTEAF
                     LFVLFLILLQQFEGNVIYPKVVGGSIGLPSMWVLMAITIGGALWGILGMLLAVPVAAT
                     IYQIVKDHIIKRQTLRNRARTYR"
BASE COUNT        13810 a         8022 c         6599 g        13410 t
ORIGIN      
        1 tatctgtttt catgatattc tccttctaaa taataaaaat agtatagcaa tattccctat
       61 aaaaacatat ttttttgcaa aaaaaattta aaatgttcgt cttatgaaaa taaaactgct
      121 tttatttctt ctggactagt ctgaccaaaa taatcttcta atgataaagt acttaatttt
      181 tccaagacta attcttcttt catagggcaa ccaatgagaa ggttttcaat atctgaaata
      241 tctttagaac tgaaaaagtc accataaata cggcaattcg taataatacc tttctcaatt
      301 tctaagtgaa aatcgtaagt tcctgacgga aaacgagtat ctctgtgata cgaatactgt
      361 ggtgagttac cataattcca atcccaattc ttatattttt catcagctag agcgtcaatc
      421 gcagcccaat ctttttcggt tagatgatag cgttttgcct gatcaatatg atcaatatgt
      481 aataattgac aaaccattaa atctttaaat tgatcggttg tgatatgacg ataatcttct
      541 gacaagtggc tacgaatatc tcctactcga cttcggacag attttatccc tttagattca
      601 atttttttcc gattaggatt taacactttt tccatagcat caaaatcaac atctaataaa
      661 agagagtaac caccgtaaat acgaccgttt actatcgtca ttgctgcacc tgatactttt
      721 ttaccatcaa taactaagtc atttcgtccc ctcatttcaa cactagaagc acctaaagtt
      781 tttagggcct taatagctgg ctcataagct cgtttgaaat caccaaattg tccatgatct
      841 ttcattagat agcaaatatt tacagcacct gagtcaacgt aaacagctcc tccaccagta
      901 tctcgacgaa caacaggaat atcatgttct ttcaaatatt cttgatttgt ctctataact
      961 gcattctgaa attttccgat ttcaacttta ggatcacaat agtatggaaa taaaatatca
     1021 tcatctaaaa aaacattttg taaaatataa acttgcatgg caagtgcaat cgcaccgtct
     1081 gtgatacgct taccatttct aattggttca ataagataca tttaggtctc cttttatagc
     1141 aatttagtta tccaataatt tcttttttcg cttcagcaat taattcagcg atatgctgtg
     1201 tcagttgcac actccttgag cgaatctttt gaggaatgcg ataatgcttg tgattaagag
     1261 taacaaataa agagttttca tttaaagaga cgaatcgcca aaacggatgc ttaataaatt
     1321 gtggcgtggt atgtccaact ccaatctcta gataaagaac tttgtcattt tgatgttgtg
     1381 ataagaaatt ttcataacgt tgtcgttgag catgaaagtc agcatcttcc accatacctt
     1441 tttcttcgtt acgtttatta atttcaaacg gttgatcaca ctttgggcat ttggggatta
     1501 agttactagg aatcttcata tccttttgtt gtgcaatcat ttgacgaatt gcctgatcat
     1561 ttcgataagt ttgttgatga caatgttgac tacactgcca taagccatat tcaccttgaa
     1621 tatgaaaaac tttttctaaa ttataatcag ccacagcgaa ggcattatca gcattagttg
     1681 taataatatg atattctttt ttcgctaaaa tatcctttaa atcaagataa gcttgtccta
     1741 ccggttgatc taaataatta agtgccacaa aacgactttg gaaggcccaa tattcttccc
     1801 aatcttcaaa gtcataaaga cttgcttgca acatatctaa tagttgatat ttagcaataa
     1861 aatccggaaa agcttcttca aatctggggc caatataggt aaatccatcc gctgcagaca
     1921 tgcctgcacc aataccaact acaatagcat ctgattcttc tattaactga cttagtattt
     1981 ctgattgaga atgattagtt ttttctagag tcttccacac tgtcatctat ttcctctttt
     2041 aataataatt tttgataaat ctcttggtct ttaggtgtga atgtattgaa aataatatag
     2101 agtttgtgct gattttcgcg ttgccactct aatatctctt gaacagccaa ttcagcggct
     2161 ctttgattag gaaaaccaaa ttctcccgtt gatatacaag ggaagacaat tgttttaaga
     2221 ttagcttgag ctgccaattg taagcaggat ttataagaag actttaataa atcctctctt
     2281 atacgagatg gcttttgatc aacttttaca taaggtccga ctgtatgaat aacatactta
     2341 caaggaagat gataggattc ggtaagttta gcttgtccta cggcttccat tcgtccttgt
     2401 tgtgtcatca attgatgaca tgctaaacgt aatcgtgaac ctgcaaacgt atgaatctga
     2461 ttgtcaatac aatggtgatt aggaataaaa caacctaaca acttactatt tgctgcatta
     2521 acaatagcat ccacttgtaa taagcaaata tccccatggt aggtaaatac atttgaatat
     2581 accgtttgat gacagacgtc tatcgttttt actttttgag tattccacca atgtgacaaa
     2641 tacctatctt ctaaagacag gtactcttga ctaatgtttt gaggatcttg ttgattaact
     2701 agaccacgcc aaaccgtttg caaatcggtg gtcctcaagt catcaacgtc tgttaatttt
     2761 tctgactgaa gatactcaat catggctagc aatagttgtt tttgattagg cattttctaa
     2821 agcttcaaaa gcagcataat caacatctgt taacacaacc aaccaatttt cgtcagcttt
     2881 ttcagaattt aacaaagttg gttcttgact tgcagctgta ttaactttaa caactttacc
     2941 tgtaagagga gataaaattg ccataaccgt ctttgaagct tcaatattta aaataacatc
     3001 atcaacttct aaatttgtat catctgtgaa ttccacatat ccaacagttc ccaagtcatc
     3061 ttgaagttcg ggagttaagc tgatagtata aagttcttca tttttttcaa ttaataagta
     3121 gttcgcaatt tttttcatga gtaatttcct ttctgagaaa acgtgattaa tataactgtg
     3181 ataataattt taaggatttg attcgttgat caagtcctgg aacaagagga atcaaaagta
     3241 gttcttctgc ttgactagca ttaactaaag cgtcaaggct ttctttaact tgttttggat
     3301 cacctacaat catacggtgg cgatttgatt taatttttgc tttttgttca ggagtcaatt
     3361 ggtaatgatt agcttcttca atagtaggaa atgttgcaaa ttcattgaag tctttatttc
     3421 ctagcatcca gatatctaaa gtctttgcta tgttttcagc ttcttctgaa gtatcagcta
     3481 taactacaaa tggagctacc atcaaattag gagacttatt aggataatat tcttcaaact
     3541 gatgataata aagagatgaa agctttttag cttctgtcaa tgggtctttg tccataaaag
     3601 gaaaaacacc gaaggtaaag cctaagccaa gtttagccgc taaataagct gatttttgac
     3661 cactccccaa cacatataag tctgggaagc tagaaagagt cggttgaact aatggttcct
     3721 tactggatga ttcatcaata agccatgact tcaattcctc cagtacctct tcgtaatcat
     3781 gtgctttatg taagctacga agtgcatttg aaactttaac tgtccctagt gaatttccta
     3841 aaccaatact tactcgattc ggatgacatg tctctaatgt cttgagagtc tccgcgagtt
     3901 taaaactact atagtgcaga ggcattatac ctccagagcc aattttgata gatttagtct
     3961 ggttagccaa atgcattatc attaattctg gattgctaat actgaatgcc ttaacaccgt
     4021 gatgttcagc cacccaaaat tgatgatagc ctagttgctc cgcctcttgt gataaaagaa
     4081 tcgtttcttg tattgcatca cttgcagttt ttccataatc aataagccca taatcaagga
     4141 cacttaattt catctttcct ccctataatt gcttcgaagt tctctaaaaa cttctcgagc
     4201 ttcctttggt aaagattctc cataatccat caaaggaaca atatctttaa aggaggcttt
     4261 tggaatcgcc aaaccttcta aatcattcac tttaatcctt aaattaatct gatcagaacg
     4321 cttagctttt attttttgta aaaattccgg atcaactact aagggcgtgg aagctcctat
     4381 catatgagca tgttgtaagg cttcaaacac ctttgaagca ctattaatac ctccagttgc
     4441 cataattgga actctatcac caaaatgttc aaatataact tgattaacca gctgcccttt
     4501 gtaaactccc tcagaacgta tagtatttct aaagacatca tgcccccagc ttgcaatggc
     4561 aagataatct acctgagcaa tcgctaaaat tttttcaaga aattccataa actcttcaat
     4621 gctatatcca atttgtgaac cgcgagtttc ttcaggcgtc gctctaaaac caaggataaa
     4681 atctgactcc gcttcttctc tgataacttt ttgtaccgct ttaaaaactt ctaagcctaa
     4741 acgacagcga ttagtcaaag tttgaggacc atactcatct tttctttgat ttgagaaggt
     4801 tgagaaaaaa gtttgaatta gtaatcgttg tgcagaagat atttcaacac catcaaatcc
     4861 agcttgtata gctcgtcttg tagcctggac atattcatcg ataatcctta gtatatcttt
     4921 gtgtgtcagt tctttaacct gatgtgggta tggcgattgc aattgcatag ggctgggacc
     4981 atatacatat ccgtgtcttg ccaaagtgtg acttgagaaa cgtccagcat gcgtcagctg
     5041 caaaacagct ttcgctccct tagacttcat tgctttagcc aatttagtca aaccaggaat
     5101 gtcctcatcc tttgaaacgc taaaaccata ttcaaataac tgaccatatt ctgtgatata
     5161 tgctgctcca gtaatttgta atggagcgga tttagcacga cgaacagcat atgctatgtc
     5221 gtcatcagtt acaaaacctt ctgatgtaga agaattagtt accataggtg atagaacaaa
     5281 acgattttct aagctcaatc cattgggaag tgttaatgga cgaaataaat taccttggac
     5341 attattcatc tctgctcacc atccccttat tagccataga cttagcctga aatctatcaa
     5401 actctgcaac taaatcgggt ctaatcatca gtaaacgatc attaaaacct acttgtttaa
     5461 ggctaatcaa catacgatca ataacatctt ctactgtcat aggacaatct aatagatcag
     5521 atagattagc cataatttca ggatctacat ttggataagc aattggacta cccgtatcac
     5581 ctagaccaat cttataaaaa tctgaaatca tttgactccg ccctttttga tcgccacaaa
     5641 cgctaaggta aattgatacc gcaataccat tctttatacg gcgctgagcc aagccagcaa
     5701 attttttgcc atttatacta agatcaaact taccaggaca ataggaggtc tctacttcaa
     5761 agtgctcaat aggttgataa aaatccgaaa atatacttct aataaaatcg accattataa
     5821 gatacccatc tgagatagac aattttctct caaaaacatc tggaataacc aatgaaaaat
     5881 ttaaaattcc ttcatcagct acaactgcga gacctccaaa attccgaaca actggttcat
     5941 aaccacgact gatgatttct tttttagcta attctaaatg tggtaattct cgatctaaca
     6001 tccccagaat gactgtctta gtcatcggcc aaatatgcaa aataatttct tgatttgagc
     6061 gattaatctc ccttaaaaat acttctgtcc atataaaagg tttttccgcg tcttgagcat
     6121 ctgtaacata gtctttaaaa atagatacag gtaactgcgc tagatcttgc cactccaata
     6181 aatctgacct cctttctcta gaaaactctg caagatttat tttatcgcaa tcgcttgaaa
     6241 ttttccagta agtttccctc aaattccatt actatcaaga agaaatatat cttttattta
     6301 cgaaaagcat ttttttatgt acaatatggc tatgaaaatt ttgattacat caggtggaac
     6361 taccgagaaa attgataccg ttaggagtat cacgaatcac gcaacaggag ctttaggaaa
     6421 aataattgca gaaaaatatt tacgagaagg acatcaggtg acactcgtga ctactaaaaa
     6481 tgcagtcaaa ccagaatctg ctaccaactt atccatattt gagattgagg acgttgactc
     6541 attaataaaa accttgaagc cattagtgaa agagcatgat attttaattc atagcatggc
     6601 agtgtctgac tatacccccg tctacatggc tgatttcgaa aaggttaaat caagcgatga
     6661 tttagatact tttttgcgca aagataacca cgaagggaaa atatcatctg aatcagaata
     6721 tcaagtcctt tttctcaaaa aaacaccaaa agttatcagc cttgtgaaaa aatggaatcc
     6781 tcaaattact ctagtaggtt ttaaactact tgtgaacgtt actaaagaaa acctttttaa
     6841 agttgctcgt catagcttaa tcaaaaataa agctacgttt attttagcta atgacctcat
     6901 tgatattacc tcaaaacatc acatagccta cctacttgat catgataatg tgtataaagc
     6961 taccaccaaa gaagatattg ctcaattaat ttacgagaag gtaaaaaaaa tatgattaaa
     7021 cgtattacac tagccgtcac aggttcaatt tcggcctata aagcagcaga ccttacaagt
     7081 caattaacta aaattggcta tgatgttcat atcataatga cacaagctgc cacacaattt
     7141 attacaccgt taactctgca agtattatca aaaaatccta ttcatttaga tgttatggat
     7201 gagcataacc caaaaatcat caaccatatc gaattagcaa aacgcacaga tttatttatc
     7261 gttgcacctg cctctgctaa tactattgct catctagcat atggcttcgc tgataacatc
     7321 gtaacaagcg ttgctcttgc tatgcccgat gaaactccaa aacttatcgc accagcaatg
     7381 aatacaaaga tgtatcataa tacaatcacc caacgtaaca ttgatatttt gaaaaaaata
     7441 ggttatcagg aaattgaacc tcgtattagt ttgcttgcct gtggtgatac cggtcaagga
     7501 gccctagctg atatctctac tatacttaaa tgtatacaag aagttgcaaa ttaggctgta
     7561 aatttgtata ctatttctag tttaatttaa ggagttataa tgaaaaagaa aaacaaatcc
     7621 tctaacattg ctataattgc aatctttttt gctattatgc ttgtcattca ttttttgtca
     7681 tcatttattt ttagtttttg gttagtccct attaaaccta ctttgatgca tatcccagtt
     7741 attattgcat ctatagccta tggacctcgt attggtgcaa ctctaggcgc cttaatgggg
     7801 gggatcagcg tagctaacag cagcattgtt ctattaccaa cgagttacct cttctcacct
     7861 tttgttgaaa atggtaattt ttattcgcta attattgcac ttgtaccacg tattctaatc
     7921 gggattattc cttatttcgt ttacaaatta ctacacaacc gctttggttt ggctatctca
     7981 ggtgctatag gctctctaac aaacacagta tttgttttat ctggaatttt tatctttttt
     8041 tcaagtactt ataatgggaa tatcaagcta atgctcgctg ggattatttc atctaattca
     8101 ttagctgaga tggtcattgc agctatcatt gtatatctaa ctgttcctcg tattctcaat
     8161 attaaacatt aaaaactggc caggaagaaa tcttcctggc ttttcttact tatattttat
     8221 agtcatattg aaaaaaacat gttataatga aagcgtttat aaatattgaa ggagtatttc
     8281 aatgacttac acagaaaatt ttcaaaaatg gttagatttt gagcagcttc ctgactatct
     8341 tcgtcaagaa ttactatcaa tggatgaaaa aacaaaagaa gatgcttttt acacaaactt
     8401 agaatttggt actgctggta tgcgtggtta tattggtgct ggtacaaatc gtattaatat
     8461 ttatgttgtc cgtcaagcaa ccgaaggatt ggcaaaatta atcgaaacta agggagagga
     8521 agctaaaaaa cgtggtgtag ccattgctta tgattcacgc cacttctctc cagaatttgc
     8581 ttttgagtct gcacaggtct tggcacagca tggaattaaa tcttacgtat ttgaagctct
     8641 tcgtccaaca ccagaactct cttttgctgt tcgccattta aatgcctatg ctggtattat
     8701 ggttactgca agccacaatc cagcaccttt caatggttat aaagtctatg gacaagacgg
     8761 aggtcaacta ccaccagctg atgctgatgc actaacagac tttatccgtg ctattgaaaa
     8821 tccatttgca gttgaacttg ctgatttaga tgaaagcaaa tcatcgggcc ttattcaggt
     8881 tatcggtgaa gatgttgata ttgaatatct tagagaggtt aaagatgtta atatcaatca
     8941 agatttaatc aataactttg gaaaagacat gaaaattgtc tatactcctc tccacggaac
     9001 tggtgagatg ttgacacgcc gtgcccttgc tcaagccggt tttgaatctg ttgttgtagt
     9061 agaatcacaa gctaaggcag atccagactt ttctactgtt aaatcgccta accctgaaag
     9121 ccaagctgcc tttgctcttg ctgaagaact cggacgagaa gttaatgcag atgtcttagt
     9181 agctactgat cctgatgcag accgcctagg cgttgaaatt cgccaaccag atggttctta
     9241 taaaaacctt tctggaaatc aaattggagc gattattgcg aaatacatcc tggaagctca
     9301 taaaacagca ggaacacttc cagaaaatgc tgctctcgct aaatcaatcg tatcaacaga
     9361 attagtaaca aaaattgccg aaagttatgg cgctacaatg tttaacgttt taactggatt
     9421 caaattcatc gctgagaaaa ttcaagagtt tgaagaaaaa cataaccaca cctatatgtt
     9481 tggttttgaa gagagcttcg gatatcttat taaaccattt gtccgtgata aagacgctat
     9541 tcaagcagtt cttttggttg ctgagattgc agcttattac cgttcgcgtg gtttaacact
     9601 tgcagatggt attgatgaaa tttacaaaga atatggttat tttgctgaaa aaacgatttc
     9661 agtaacactt tcaggagtgg atggagctgc tgaaattaag aagattatgg acaaattccg
     9721 tgaaaatggt ccaaaacaat ttaacaatac tgatattgta ttattagaag atttccaaaa
     9781 acaaatcgct actaaaaatg atggaacaat atctaactta acaactcctc caagtaatgt
     9841 tttgaagtac acgttagctg atgattcatg gattgcagtt cgcccttcag gaactgaacc
     9901 aaaaatcaag ttctatattg ctacggtcgg taatgattta gcagatgcag aaacaaaaat
     9961 cgccaatatt gaaaaagaga ttacaacatt tgtaaattaa taaaaaaagc taggaactta
    10021 ctcctagctt tttttatatc tatctaacga tgttctatta ttttcaatca tgaaatataa
    10081 ctgcaattaa cttcacgatc aaataataga gcagacttac aattaaccac gttatcccaa
    10141 taactgcaac taaacctatt aaaacagata ataccgtatt cttagttgct agttggttac
    10201 tgataaacca tgaaactgca gaaataaata tcagtaataa tagttgaagc ctttttttca
    10261 tataattcct cttactactt ttgtgcattt tgcatttgat aataaatacc tttttgagct
    10321 attaattcag agtggttacc cacttctaca atttcaccag ataccataac taaaataata
    10381 tctgctgttt gaatggtaga taagcgatgt gcaataataa agcttgttct ccccttcatc
    10441 aacatttgga atgcttcttg aacaagaact tcagtacgag tatctataga cgatgtcgcc
    10501 tcatctaata tcaatattct aggtaattta agaaaaatac gagcaattgt caatagctga
    10561 cattgcccct gtgataacga atctccagca tcttctaaat acgtatcata tccattcggt
    10621 aattgtttga taaagaaatc tgcattagct gcttttgcag cctctatcac ttcttcacgg
    10681 ctagctttag gattggcata tgcaatgtta tcgtgaatcg tagcactttt taaccacgtt
    10741 tcttgtaata ccattccaat ttcttgcctt aattgtgatg gctcataatc agtaataggc
    10801 ttaccatcta aaaggatatt tcccccgtca acttcataga aacgcattaa taaattgatt
    10861 aaagtagact tcccagcacc tgttggtcct acaatagcga ccttagctcc tgctggaatg
    10921 tgtaaattaa taccattcaa tagtggtttt gatttattat aaccaaaaat aacgttttta
    10981 aaatcgattt gacctttgac cgttgatgaa tcaagttttt ctgtacctgt tatatttggt
    11041 gacgattctt ctaaaataga gtaaaggcgt tctgcacaag ccagagcact ttgcatctca
    11101 gacaggacgg aagagatgtc attaaatggc ttagtatatt gattgacata atttaagaaa
    11161 gtgattaatt gaccaacact aaaagcacct gacataatac gaagggcacc aatccctgct
    11221 aagaagccat aaatcaaact attgataaat cgggttgatg gattgacagt tgaagaataa
    11281 aaaattgctg attgagaaaa gttagcatac tcttgattaa tagtcctaaa atgatcacta
    11341 ctttcttctt gcgcactgaa tgcttgaata aggctttctt gactcaccat ctcttcaatg
    11401 aattgagttt gtcgtcctcg tgatgccgtt tgattttgat ataagtgata actcttttta
    11461 gcaataaagc gggctagaaa gagtgataaa ggcgtcagaa ataataccaa aaaaagcatt
    11521 aataaatcaa tctttgccat actaaaaata gtaactagaa tagttaataa accaacaaag
    11581 aactggttaa aaaccattaa taaaccattt gataattgct ccgtatcggt agtcacacga
    11641 ctaatcaagt cacctatgcc acgtttatca aggtaagcta taggtaatag attcaacttt
    11701 tccattacag cctttcttaa agaagcaaca taatgaaaaa ttaagcggtt atagagcaga
    11761 ggattaatcc attgaataat agtatttgct aaaataactg caaccatctt ccacattata
    11821 ggtaacaata atatcataga atgaggcgat aatacaacgt ctaccgcctg tccaattaga
    11881 acaggcaaat aaacagtgag gcaaacttgt actaccgtcc ctaataatgc cattcctact
    11941 aagctttttt tagaaagaag atcttgaaca agcttttttt gtatcttttt aggatgactt
    12001 gctttcattt actcctcctc cttaaaattt tgtgaatggt aaatggactt ataaactttg
    12061 ttatgagcta ataaagattg atgattatca aaaccaactt gtttgccctt atcaaggagt
    12121 aagattctat ctgcattttg tatactatta attctctgtg atacaataat aagattagtt
    12181 tggttgaaat gtttaattat agctttaaat aaacgagctt ctgttaaata atccaatgca
    12241 gatgttgcat cgtctaaaat caaaaatgga atcttatctt gaactaaagc tcttgcaatc
    12301 gtcaaccttt gcctttgacc accagagaaa ttcctaccaa aactctctac aggggcatca
    12361 agttgaccat ctttttcttt tacaaaatca ctagcttgtg ctatttctaa agcagtccaa
    12421 agtttttcct cactaacttt accaagtccc aaagaaaggt tagaacgaat agttccttta
    12481 aacaactgag cattttgagg aacaactctt accaggctac gccaatttga aattgtatct
    12541 ggactttttc cctgatgata tatatcaata tccccttctt gcactttata aatatgaagc
    12601 agtaaattaa ccaaggttga tttcccagag ccagttccac caattatccc taaagtttct
    12661 cctacattca tagaaaaagt aatatcagat aaagcttttt cagaggagtt tggataagaa
    12721 aatgccattt cttgcacttc taaagcctta ttcgaatatt ttggctcaag tttgtcatca
    12781 attatttcag acggtctttc aaaaacggct ataattcgct tagcactgat ataactttgg
    12841 ttcagtgatg tcaccaacat tgtcattttt aacaattcaa caagaatttg caataagtaa
    12901 ttaattaaag caaccaacat tccttgtgat aaaagatgat tagctatatt taaattacca
    12961 cgccaaataa tcacaaccaa agtgatatta acaactagaa atgttagagg cgttactaaa
    13021 ctagaaagcc tacctgcttt taattgtaaa ttcgtatagt gataattgat atcattaaat
    13081 gcttcactct ctctatctac ttgattaaag gcacggataa ctcgtacacc ttgaagttgt
    13141 tgcctagtca actttaccaa ataatcggtt gaagttctga ttttaaggta aataggattt
    13201 aatagtcgag acattacaaa aacaatgata aataacgtga caaccattat caagaaccaa
    13261 atagtcaatg agggactaat ggaaaaagcc ataattatgg caccaaaaac aataatcgga
    13321 gccctcaaaa ataaacgtaa aaattgattt aaaccagttt ggatgtgaaa tgtatcagca
    13381 gtcaaacgag taatcaaact agcagttccc aattcatctc tgtctttttt acccagtgac
    13441 atgacttttt gatagagatc ctctgtcaat tgtctcgtat aaccaactgc ggcttttgaa
    13501 gaataatatt gagcagttat cgctactaca acacccaccg cagccaaaaa gaaaatcaat
    13561 ccaatttgca acagtaaact acttctatcc cctcgtggaa tggtctcgtc aatcattttt
    13621 gcaataacga ttggtaccaa aagttcaaaa gaggcttcta ataatttgaa aaggggtcca
    13681 aagatagttt ctttgatata ccccttaaaa taataagtta gtcttttcat acttaccctt
    13741 cttaaaacaa ctccattatt tttatcaaat aaaggttgaa ttgaacttca cgtgagtagt
    13801 aaatattacc accattttta tagagcttcc ctcctgaaat taaggcgaga ggatgatagt
    13861 aaaagtaagt gtccccgtta taatttccta ggctgggagc tacaacattt ttagagtaat
    13921 ttttggccaa ctcaatggaa ttttcacccc catgatccgc tacataatca atataagaag
    13981 aaccaaaatt atatgcttgg acagcagtcc atacatcaac cttagctttt ttagcttgac
    14041 ttatattttg tgacaacaga gtgacgccat gtttaatact ggtgtgacta tccgtaatac
    14101 tgttcgtagt tccactgata ctttcacttg attgcatcac atctatagca tctcccttag
    14161 tttcagtata aatcatagat aatactaagt gcacattagc ctctgaacca ttctctgcaa
    14221 gagtattctg cactaaatca tgatattgaa gcactttttt aacattttca tggatgataa
    14281 ctagacggta cccaatgaaa ataataataa ttaatgctat caaacgtttt aaaaatttaa
    14341 acatattatt tattttgaat atcctgtatg tttttgatta gtattgagta tgtaccatca
    14401 gggttttgga taaattcaac tgattcggca tcttggtaga cattgttggg tacaatcaat
    14461 tcaataccat ttgaaagaga tagcttctga ttctctaact tcttaatttg acgggaatga
    14521 tcaatatcac ttacttgaac agatccaggt attgcgtctt tcaattcatc aacaaaagtc
    14581 agcctggctg ttaaattatc atcaaataat tgatctgcta atttttctgg agacaattct
    14641 tgctcttctt ctaaattctt ataaatagcc gatttcattt ttgattggaa agaaaaatca
    14701 tctttgttga aattttcagc aattttttga gctgtctgtt caaccatttt tatagatttt
    14761 ttaacagact gttctggttg tacttgtaat aaattttcag aaaaataatt agcaaatgaa
    14821 ccattgtgtt tgatacgctt ttcaattaaa tagtattgct ttgatgactt gttaacaacc
    14881 aaagcttcat ctggagtttg agcagcactt ggaaggttat tctgtgttat tgtaattggc
    14941 tgctcttgat tttcagaaac atgagcaaat tgttccttta atgagattcg aaggaaagca
    15001 aaatgttcca ttccatcctt gtcaaattga ataaaaacta agtcattcgt tttttgatct
    15061 tccgatatga caaactcttc tttccaaagt tgcgcaattt gacaggaaga aacatataaa
    15121 tcatcttgta aatgactcat aaatacatta tcctctccaa aatagccacg cttagcttca
    15181 tcagaaaaaa ctttacttaa ttttttacga aagtagtcat caatccttgg agttagggtt
    15241 aacggagtat ctgatagcac taactctgtg tcgttaggag aaaactgatg tataataatc
    15301 tgtttgatat aaaaatccat cataacatta attctcatac aaagggaaag cgtccgttaa
    15361 tgatttaatt tcttgacgaa cttctgtgag gacatcttga ttttcatgat ttttgagtgc
    15421 cttaatcatt aattcagcga ttcttcgaga ttcttcaact cccattcctc gactagtaat
    15481 tgctggagta ccaattcgaa tacctgatgt tttaaatggt gatagtcgct caaacggtat
    15541 agagttcttg ttaagggtga tattgacttc ttcaagaaca ttttgtgcct ttttgccatt
    15601 ctcaataact tttgtgacgt ctactaagaa caaatgatta tcagtaccat cagaaatcaa
    15661 atgaaagtca tcatcttctt taaaaacttt agccatggct tgagcatttt taataatatc
    15721 ctcaccgtat attttaaagg aaggatctag agcttctttc aaagctacag cttttgcagc
    15781 aattacatgt tctaatggtc cgccttggag tcctggaaag actgcagaat taattttttt
    15841 agcaattgct tcatcattgg ttaaaatcaa accacctcta ggtccacgta gagtcttatg
    15901 tgttgtcgtt gtcgttacat gagcataagg aataggacta ggatgatgac cagaagcaac
    15961 taaacctgca atgtgagcca tgtcaaccat taagtaagca tctactgcgt ctgctatttg
    16021 acgaaacttt tcaaaatcaa taatgcgaga ataggccgac gcccctgcaa caattaattt
    16081 aggttgcgtt tcctgtgcta ttttcaaaat gttatcatag tctagcatct ccgtcttagg
    16141 atcaacagag taagaaacaa aatgataagt ttttcctgag aagctaactg aagcgccatg
    16201 tgttaagtgc ccacctgctg ctaagtccat ccctaagaca gtatctcctg gctcgattaa
    16261 agccatgtaa gctgctgcat tagcctgact accagaatgt ggttgaacat tagcaaactc
    16321 agcattaaat aacatcttag ctcgttctat agctaaactt tcaactacat caacacaatc
    16381 tgttcctcca taataacgat gactaggata cccttcggca tatttatttg tcaaaacaga
    16441 accttgtgct gccataacag cttttgaaac aacgttttct gatgcaatta attcaatatt
    16501 attttgttgg cgaatttcct catcatgaat agcctgccaa agttcttggt caaattcttt
    16561 aaaattgtct ttatcaaaaa tcatttttta ctccttaagc tcgttataac ttttaaataa
    16621 tggtttcaat cattatattt atcacatttt tactattagt tgcaagatga ttaaattaat
    16681 tttcaaaaat taattcaggt atcgtagccg ttagtacctc ttgcgtgatt gcaccttgcc
    16741 ttaaaatctt aactctatca ccagacaaat caataatagt agaatccaca cctgtcaatg
    16801 ctttatcatc ttcaatacct aaaacttgat gattaaactg tttttgaatt tctgagaata
    16861 cacgaccact atcacatcca gaaatattag cagaaggacc aatcaatgga ccatggttag
    16921 caatcatatc aagggttaca gggtgcttag gcatccgaaa tcctacgctt gataacccag
    16981 aattgatcca atccggaact tcttgtgaag cttccaaaat aatggttagc ggtcctggta
    17041 aaaaagcatc gtacaattgc ttcaaatagg tcggttgatt tttagaatat tttaaaatag
    17101 tctcaaaatc acatatattt aagttcatag ccttatcacg aggtcttttt ttaagctcat
    17161 agacgtggtt cacagcttct tcactcaaag ctttggcaaa gattccgtag accgtttctg
    17221 ttggcataat aactgcgcca tgatcctcta aaatctgtcc caaatcattc attatctaat
    17281 accaccattc tatctttacc aaatatatct ttcaaaactc tgcaacgatt gtttggaaaa
    17341 tatcttttca ataaacttcg taaatcatca ccttgtttgt aacctatttc aaaatataat
    17401 ttacctctag gttgcaaata ttctcttgag ttttcaatga ttttacgata aatagctaat
    17461 ccttcttcgt ctgcaaaaag ggcactatga ggctctgaag ctagcacatt cttccccacc
    17521 tcgtctttgt cattgtaaga aatataggga ggattagaca caataatatc aaatttatct
    17581 gaaatgttac taaagacatc tgattctata aaagtcactt ccgcatcaca attattagca
    17641 ttttcctttg ccaagtctaa tgcagataca gaaatgtcac tagctagaac atcccaagag
    17701 gggcgttctt tttttagaga aatggcaatt gctccactac ctgtaccaat atctaaaaca
    17761 gaacaatctt ctactttatt ctctgataag attagatcaa ccaactcctc tgtctcaggc
    17821 ctcggaatta aaactcgttc atcaacaaag aaaataagat ctctaaagta agctttacct
    17881 gtaatatact gcggtgatct atgtttttta agctgttgaa aaatattttc cattaattgc
    17941 tgatcagtat cactcacact actattttga tttaaaaggt aatcagttgt tgaccattgt
    18001 tttaggtctt ttaatacata aataaaattt tcgacttctt caccacaagc ctctaacaac
    18061 tgttcataat gcttgattaa ctgcgcataa ttcattattt atttaaggct tctaactttt
    18121 gagtctgatc atacataaca agtgcatcaa taacttcatc catttttcca gataaaatag
    18181 tatctaattt ttgaagtgtc aaaccaatac gatgatctgt tacacgattt tgtgggaaat
    18241 tataagtgcg aatacgttct gaacgatcac cagtaccaac tgttgattta cgttcagcat
    18301 cttgttcatc ttgagcaatt tgcgcaaaat ggtcagcaac acgagcacga atgattttca
    18361 tcgctttatc acggtttttt tgctgagtac gctcttcttg catttcaact ttgattcctg
    18421 taggaatgtg taccatacga actgcagtag caactttatt gacgttctga ccaccagcac
    18481 cagacgcgtg gtaaatatcg acgcgaagat ctttttgatc aatttcgtat tctacttctt
    18541 caacttctgg cattaccaaa acagttgcag ttgaagtatg aacacgaccc tgactctctg
    18601 tcaccggaac acgttgaaca cgatgagctc ctgattcata tttaagctta gaataaactg
    18661 actgaccaga aaccatagca accacttctt taataccacc aacaccatta acggatgatt
    18721 ccataacttc aaaacgccat ccttgagttt cagcatattt ttgatacatt gttaaaagat
    18781 caccagcaaa aagcgctgct tcatcgccac cagcagcacc acgaatttct aaaataatgt
    18841 ttttatcatc gttaggatct ttaggtaata gaaggatttt taatctttct tcatactctt
    18901 ctttagctgc ttttgattct ttaagttctt ctttagccat ttcttccaat tcagcatcac
    18961 cagaagcatc cttgatcatt tcttcagcat ctgaaatatt ttgaatgact tgcttatatt
    19021 ctctataagc tgtgacagtt tctcgagtat tagcttcttc acgtgataat tccataaatc
    19081 gctttgtatc tgaaacaaca tctggatcac taagtaactc acctaactct tcataacgat
    19141 cttcaactgc ctgcaattga tcataaatat tcatttttct ttgtatctcc tttaaaaccg
    19201 attatctttt aaatatctgg attaaaataa tgttttcgac aaactgggat atatgtttca
    19261 ttaccaccaa tttgtatctg atcaccttca tagacaggtt ttccgttttc tgtccttaaa
    19321 accatggttg ctttcttact acagtattgg caaattgttt tgatttcatc aatcttatcg
    19381 gccaataata gcaaatgttt tgaaccttca aaaagattgt tttggaaatc atttttcaaa
    19441 ccaaaagcca tgactggtac atctaagtca tctactactc tagctaaatc gtagacgttt
    19501 ttcttactca aaaattgaca ttcatcaatt aaaacacaat aaggcttttg tggtaaattc
    19561 tgaatatagg agaaaatgtc catatcatct gaaattggaa ctgcttcacg acgcattcca
    19621 atacgacttg aaacgactcc aaattcatct cgagtgtcca aggcgcttgt cataataacc
    19681 acaggttttc cttgttcttc atagttatga gcaactttta aaatttcaat ggttttacca
    19741 gaattcattg tcccatattt ataatagagt tgagccaaag cggaaaatcc tactttctat
    19801 agtattctac ctattttacc acaaaggctg aagttttccc agccattgaa aacagtgtgt
    19861 actttttcac aacattatgt tacaatgatg cccaataaca taaaggagta atattatgcc
    19921 atttgtaaaa attgacttgt ttgagggacg ctcacaagaa caaaaaaatg aattagctcg
    19981 cgaggttact gaagtagtat cacgcattgc aaaagcacct aaagaaaata tccatgtttt
    20041 tatcaacgat atgcctgaag gaacctacta tcctcaaggt gaacttaaga aaaaataaga
    20101 aatagtaaaa gaggttaccc aggtaacctc ttttgatagt ctttgcaagc atatttcttg
    20161 acaaattgaa aatatttgaa aataagtatt gattttcaag ttagaaaacg gtatcattag
    20221 aatatattgc tttgggggtg gaaaatttgc aactttctca tagtttaaga ctcatgggaa
    20281 cgactatcga tattcaaatt aattctaaaa atgctcaaaa acaaatccaa gaggtcatag
    20341 aactcttgga gctctacaaa aatcgcttta gtgcaaatga ctttaattca gagttaatgg
    20401 ctataaataa caacgctggt atcaagccta ttcaagtaca tccagacctc tttgaattaa
    20461 tcacaattgg gaaagaacac agcttagcta ggcctagtaa cctaaatatt gctattggtc
    20521 ctttagtgca aacttggaga attggatttt cagatgctaa actacctagc cctagtgaag
    20581 tatcagaagc tatgatttta tctgacccaa ctcatatact tctagattca aaaaatcaat
    20641 ctgttttttt aaatcagata ggaatgaaaa ttgatttggg tgcacttgca aaaggatata
    20701 ttgctgacaa aattatgacc tatctcaaaa atgagatgat tgattctgct atcattaatt
    20761 taggcggaaa cgttttagtt cacggagata atcctaatcg ttcagaaggg tattgggtta
    20821 tcggcattca acatccgaaa aagaaacggg gacaaaatat tggagctgtc aaaattaaaa
    20881 accaatctgt tgtaacctca ggtacttatg aacgccgtct gattattgac gataaggaat
    20941 accatcatat ttttgatcga cagactggtt atccgataca aacagagatg gctagcattt
    21001 ctattgtttc taaacaatcc gtcgactgtg aaatctggac cacaaggttg tttggcttat
    21061 ccattaaaga ggctcttgat attttaaacg ctgtttccta cattgaaggc attatcatca
    21121 ctaaagatga ccgtatctat ttatctgatg gcttaaaaca tcattttcaa ctattttatc
    21181 attaagaaag aggatttcta atatgaaact tattggcatc gttggaacta actcaaataa
    21241 atcaacaaat cgtcaattat tacaatacat gcaacaacac tttgctgaca aagcagaaat
    21301 tgaactcatc gaagttaaag accttccgtt atttaataaa cctgctgata aaaatgttcc
    21361 ccaagtaatt ttagatattg ctgctaaaat tgaagaagct gatggtgtta ttattggtac
    21421 gccagaatat gatcattcta taccttcggc cttaatgagt gttttagctt ggctatcata
    21481 tggtatttac ccacttctca acaaacccgt tatgattact ggtgcttcat atggaacact
    21541 tggatcatca cgtgcacaat tacaattacg tcaaatactt aacgctcctg aactaaaagc
    21601 aagtgtttta cctgacgaat tcttgttatc acactctctc caagcctttg ataaagatgg
    21661 caacctacac gatatcgaaa caagtcagaa gctagatgct atctttgatg attttcgtct
    21721 ttttgttaag attactggta aattatcaaa tgctcgtgat ttacttcaaa aagaagcaga
    21781 aaattttgac tgggaaagtt tataagagta tagaagggaa taactaagat gaaatttgta
    21841 ggtattgttg gctcaaatgc agagcaatca tataatcgta tgttgcttga atttattcgc
    21901 aagaatttta aaaccaaatt tgaattggaa gtccttgaaa tcgatgatat tccaatgttt
    21961 aatcaagtcc aaaattggga agaaagtttc caacttcgtc ttcttaataa taagatcaca
    22021 cgagctgatg gcgttattat tgccactcct gaacataatc atactattac ggccgctctt
    22081 aaaagtgtgc ttgagtggct ctcatttgct gtgcatccac tcgaaaataa acctgttatg
    22141 attgtcggtg cttcatatta tgatcaaggc acatcacgtg ctcaaataca ccttcgtaaa
    22201 atccttgatg ctcctggcgt taatgcttac acacttccag gaaatgaatt cttattagga
    22261 aaagctaaag aagcctttga tgataatgga aacattatca atcctgggac tgttcaattt
    22321 ttagaaactt gtcttgataa ttttatcaaa tatgtaggag ttgtatcatc attgagaaaa
    22381 cctaaagcta ttgaatctga agacctctac tgtcagggac aaatcgatac aaccattact
    22441 agtatcgatc cagatgatcc tgattggtta gacaaggcag ccgagaaagt taacgccgtt
    22501 gaaggggatg cttacgttaa actagacaat ggtttactta ctgttaacca acttaatatg
    22561 tttttaaatg ctatgccatt tgagctaaca tatgcagatg ataataatca atttctatat
    22621 tacaataata atcatccaga tccagataca atgttaggaa aacgtgttcg tgaacaagta
    22681 ggaaaccgta tggccacagt tcatggtagt ttaccaccgg cacgaatgaa aaatgttgaa
    22741 tgggttatcg gtactcttcg taatggaaat caagaatatg tacgtacagt cgttcctggt
    22801 acacctccag aagttatcaa tactcacaac tatcaagcta tgtactaccc agacggatct
    22861 tatgctggta ttaatgaaat tatctttaac tttaaaccgt ggttagattg gtacctccaa
    22921 actactggac aacgtttggt cggtggaact actgctgctc ctggagcttc agcagttgac
    22981 gctacttcta gcgcttcaac tagcacagag gatgctcccg ttgatcaagc cacagatgcc
    23041 acttctggtg cctctgaaca ttaatctcct cgcttttgag gatctgatac cttaaaaaga
    23101 tatcaagatc ctccttttta atatttaatc aagttaataa aatcacataa aaggaatata
    23161 ctatgtcact ttttcaagaa aaaattgctt acaattgcgc taaaaaggaa gcgctttata
    23221 aagagagttt aggacgctac gccttgagat caatgctagc aggggcttat ttgacaatga
    23281 gtactgctgc cggtatcgtc gcagctgata ctattggtaa aatttctcct gctctatcag
    23341 gttttgtatt tgctttcatc tttagttttg gacttattta tgttttaata tttaatggtg
    23401 aattggcgac atctaatatg ctttatctca ctgcaggagc ctataataaa aatatctctt
    23461 ggaaaaaagc cataacaatt ttaatttatt gtactttttt caacctcgtt ggtgcttgta
    23521 tattagcttg gttgtttaat caatcgtact cattccagca tttaacaaat gatagctttt
    23581 tagggcatgt cgttgctaaa aagttaggaa aacctagttc aggagctttc ctagaaggta
    23641 ttattgctaa tatgtttgtg aatttagcta tccttgctta catgttatta aaagaagaat
    23701 cggcaaaaat gacggttatt ttatctgcta tttttatgtt tgtttttcta tcaaacgaac
    23761 acttaattgc taattttgca tcctttatgt tagctgcttt tagccatatc gaacatatta
    23821 aaggttttac tcttctaaac gttatccgcc aatggacact tgtatttttt ggtaactgga
    23881 taggaggggg cgttttcatc ggcctagcct atgcttggtt aaataaaaca aaaacttctc
    23941 accatgaata aaagaaaaac tgagtaattt attactcagt ttttctttta tattctcatc
    24001 atgaaatcaa taactaaaaa ttaacttttt tgataaataa aggaaagtat catattaatt
    24061 ttttaggcaa tccattcaaa atgatattta aaacaacagc tgtcagggtt gagataacaa
    24121 taccatttgt caaaaacatt tgtaaagtat taggaagact aatgaagaga tttgtctcat
    24181 taaaacctac tcctgctgca attgatacgg ctgcaatgat aaagttatgc tcattatgct
    24241 caaaatcaac ctgattaagc attttcatcc cttgaagtgc taccatacca aataaaacta
    24301 acatagcacc accaagaact ggacttggaa tcatttgagc cattgcgcca aatttaggta
    24361 acaagccaag tataactaag aagagagctg taaaatatat cggcttacgc gtacgtatcc
    24421 cagatatctg taccagtccc acattttgag aaaaaccagt atatggaaag gtattaaata
    24481 agccgctaag taatactgcc aatccttctg aacggtaacc attacgaagt ctcttactgt
    24541 ctaatttatc gtttgtaata tctgaaagcg caaggtaaac acctgttgat tctaccatag
    24601 aaactgttgc aataatacac atcattaaaa tagaagtaaa ttcaaatcta ggagctccaa
    24661 aataaaatgg cttcggaata tgtacaagtg gtgcttctgc taccacagaa gcatcaacta
    24721 agcccataaa tgctgcaaga attgtacctg aaatgagtcc aataaggatt gaaattgact
    24781 tcaaaaagcc ttttgcgaaa atattaatta ataaaacaac accaatcgtt accagtgata
    24841 aagttaaaga ttgtaagctt ggttcttttg cattatcacc catatttccc atcgcaactg
    24901 gtattaatgt caaaccaatt gtagtaataa ctgatcctgt tacaattgga ggaaagaaat
    24961 ttgcaacttt agaaaaaatg ccagcaacta aaacgacata tattcctgaa gcaattaaag
    25021 ctccaaacat atagccagaa ccttgttgtg caccaataat tgataaagga gctacagact
    25081 gaaaggcaca ccctaataca actggaagac ccactccaaa gtgcttactt aatcgtaatt
    25141 gtaataaggt ggcaatccca cacataaaaa tatctgtcgc aataagataa gttagttgct
    25201 tagcattata accaagagca ctagcaatca tgataggtac aagaatggat cctgcataca
    25261 tagctaacag gtgttgcaag cctagaagtg ctgcttgcga attactttgc tcattaaatt
    25321 tcattaagca tcagcctccg caaaaacaac tcgaccattt tcaaacgctt taatacgagc
    25381 aagagaagta accggaacac ctgttttttc taacaaatca cgcccatctt ggaaagattt
    25441 ttcaataacg attccgatac cagcaacctt agctccagct tgaccaataa tttcaagtaa
    25501 tcctttagcc gcttgaccgt ttgctaaaaa gtcatcaatg atgagtacag tatcatcgtt
    25561 agataaaaag cgactcacaa tagaaacttg actcgtaact tgctttgtaa aagaatacac
    25621 ttcagcagtt aagatacctt cagtcatagt aatgttctta gcttttttag caaatatcat
    25681 tggtacgccc aatgcttgag ctgcgtacac tgctggcgca attccagatg cttcaattgt
    25741 aacaaccttc gtaatgccgg cttctttata tttatcagca aaaactttac ctatttcctg
    25801 cattaactca aaatttacct gatgagtcaa aaaagaatca acttttaaaa tgttctcacc
    25861 taaaacatca ccatctttaa gaatacgttc ttccaataac ttcataataa ccccttcact
    25921 ctttagaaaa taaaaaaata acttacctca atcagaggac acaaatctcc tctaatttat
    25981 cgcttcatta acatgaacca acaggcaagc tatttattag cttacttatt gttagacatt
    26041 acggtgtctt gtagagacgc taagccaatc ctcagcataa ataagtaaat catatattta
    26101 attgttatgt atgatagata actattgtaa catttttaat taagatttca agatttaatt
    26161 taaatggaat caccattcca aatagaattt ttaacaataa cgtaatcaac gtgtctcaag
    26221 ctatccaatt cttttccacc tgcgtaagaa atagaacttt gcaaatcttg ttgcatttct
    26281 gtcaaagtat cctctagtcg ccctttaaca ggtaatagaa ttttcttacc ttcaacattt
    26341 ttatgttctc ccttttgata ttcagaagct gagccatagt attctttaaa ttgctgtcct
    26401 tctacttcca caagttttcc aggactttca aggtgaccag caaacagcga accaatcatt
    26461 accatactag caccaaagcg gatagatttt gcaatatccc catgtgttcg aataccacca
    26521 tcagcaataa ttggcttgcg agcagcttta gaacaccaac gtaaggcagc taattgccaa
    26581 cctccagtac caaaacctgt cttgacttta gtaatacaaa ctttaccagg tccaatacca
    26641 actttagttg catctgcacc tgcattttcc aattctctaa cagcttcagg cgttcctaca
    26701 tttccagcaa taacgaaagt tcctggaagc tcttgtttaa tgtgttggat catctctata
    26761 acactgttag aatggccatg tgcaatatca atagtgataa actcaggagc atcttctttc
    26821 agacttgtca caaaatcata ttcatagtct ttgaccccaa ccgaaatcga agcaattaag
    26881 cctttatcat gcatacgctt gataaaaggt cttcttgctt cttcattaaa acggtgcata
    26941 atataaaaat aaccttcaca cgctaatgtt tcagctacct cttcatcaat aatagtctgc
    27001 atattagcag ggattactgg aagtttaaag gtatagtttc caagtttgac acttgtgtca
    27061 gcttgtgagc ggctagatat aatacacttg tttggtatta attgaatatc ttcataatca
    27121 aaaacaggaa tatcgttaaa cataatggtt ctcctaatga gtaaaatgac cttcctataa
    27181 ctttgtataa caaagcatcg ctaggtaaaa acataccttt cctttttaaa ttatatactt
    27241 tatttatttt tagtcaattt ttgatcaatt tatttgaata atgttcgttt ttttcaaaat
    27301 aaatgtaaaa tcctatagag cagtactcta taggatttta catttattat attgcttttc
    27361 tttttcgaag ataagttaat cctacaatag ctaagataac aacaacgata acgaatagaa
    27421 ctgcaaattg actaccaatt atagctccat ttttcggaat agctacttgt ccgatggtaa
    27481 aaatttgtga ggctactgcg gttgcaaaag ctcccgcaaa ttgttgtagt gtatttaaga
    27541 tactattacc atccgctgac tgtgctggct ttagttttgt taaagctgtc gtaaccatat
    27601 tacttgctcc aatccccata ccaattttag taagaatatc aaaggctaaa agtactacaa
    27661 caaacttcgc aggaatgaat atagctgtac caattaaacc tatcaaagaa agggagatac
    27721 ctatcataat tggcttaaat gcaccaattt tatccaggag ataacctgca aaaggggata
    27781 aaaaaactac tgctattgca cctggaaaca taaacaaacc agctttggtt gaagtttgat
    27841 gtaaaacaag ttgcaagagg ttaggtaata agaacgaaag tgctagagga atcatttgaa
    27901 aagccaggac gccatacaat aagattgaga aatctttatc tttaaaaaca ttcaaattca
    27961 aaaattctag ctgtttattt aaatagtaaa aaataacaaa actaacaata gctactaata
    28021 agtaaaaaat agacatcttt tcaatagcta aaagaagggt tgccaagcca atacctaaac
    28081 tgacaaaggc aggaaaattg agtttatcat taattcttac ttgacgtttg ggaatagaag
    28141 ccaaacctat aaaggtagat atgataagta ttggtgccag aagcataaaa atcatcttcc
    28201 atcctaacat tcctgaaatg acaccaccat aagtaggtcc aactgccggt gctaaaagtg
    28261 tgaccatagc agctactccc atcattagtc ctctacgctc catcggaatt tgtgtaagaa
    28321 tgatatgaaa caataaaggg agggctaatc ctgtcccaac accttgcaag actctcccta
    28381 ctaacaatat agctaaatta ggggcaaagc tatcaattaa aacaccaaat aaaaaaagca
    28441 catttgccaa tgtaaaaatt tgtctctctg ataaattttt aatcataaat gctgacaaag
    28501 gcacagtcac agcaacagct aacaaatttc ctgttgtcac ccattgaata acagcggggt
    28561 taaccccaaa ttctttcatc aaaagcggaa aagtgacatt catagatgtt tcaataagaa
    28621 tccctgaaaa acctaatata gccgttgtaa ttatggcagg cataatacgc catccctttt
    28681 tagtcattaa aatctcctta ctttgattat tatcataaaa tagagcaccc cgctaaatca
    28741 aggtgctcca tttaattcat tcgaattaaa attaaattgt ttatattagt tgtcaaagga
    28801 caatcaacag ctcttattat atcaaataaa gctaagaaaa acaaagagtt ttttgaatat
    28861 taatgccttc actttcttat ctattctcta cccatcaatc accacttcag agaacataac
    28921 aattgcaatt tactcttaat accgttatca tgttcatatg ctaataccca agaaaatagt
    28981 ggcttttgcc tcaaatgaat ctgaaaataa tttctttatt acgttaaaga cttactttaa
    29041 ttatttgttt tctattcaaa tcattacaga tatttcaact ctcaatcatg cagattttga
    29101 cggtagcttt gcattccatg atatagaaac tagcatacct catctggtaa ttgactccaa
    29161 ctacttagcg attagtcaga ctaactcaaa aacagaggct aacgatatta aaacgttctc
    29221 ggaactctcc aaaacaatga ccgagtttta ctatatgcta aattttgact tatttaacca
    29281 tctcccatat cgttttagat tgcataataa ggacggtcaa acaatttatt cgaaccataa
    29341 acctgaagat ccttttgata tttatcctga agaagaatat cctatagaca aatgggttca
    29401 aaacagtcta atagagaaaa aagcgaaaga gctacattta ctcttacctg gcgcatcaca
    29461 agattatatt ctagtacaat cttataaacg cttagaaaat gatagtgggc aactagttgg
    29521 ttatattgaa catgtccata atattaaacc actattagag gggtatttaa aagaatccgg
    29581 tcaagctatc gttggatggt ctgatgtcac gtctggtgct tctatctcta acgatgattt
    29641 tgaactataa tccactcgtc actctaaaaa gaaaactaat aactaaaagg cactggttta
    29701 attccagcgc cttttattaa tcaatctcca gttgttgtaa gttcaatttt ttctttaatt
    29761 ggtagacctc gattagctgt aaaaatcaaa ggaactactt ctgttgtaac ttcggaaaat
    29821 tgtagaccaa accatcgtat tggtgttgct gtccaatgct ttacagatga tttcattagc
    29881 ataacaaatc gttcaccagg ttttaggttt tgactgcttg aaaaacgttc atcaaggaca
    29941 ataaacctaa aatctcctac tttactatca ggtctaacgc tgtatgtctt tccttgttta
    30001 ggcagacgtc cactttcaag taattcttct acaatcgtac gaagatagcg tgacacagtc
    30061 tgacgcatct taaatccaag gtaaagttct accttcacaa tgaagtcggt tcccatcatg
    30121 tcaactttat actcagctgt ataaggttca tcagtgacct taatgtttac aaaccaataa
    30181 acttgtgctt ttttagggcg cttatcaagg atagaataca tgatagactt atcaatcatg
    30241 tctccttcca tacgatttgt caaataaaca acgttagtat gataaatcgg ataacgatga
    30301 tcatgtctaa gtttatctaa ttgtccgatg taatctttta aatccaacaa cttaacataa
    30361 cgcgaaacaa ttttactacc tttataccag atagtcatga tgaatataat tgctactgct
    30421 ataataacaa caacatagcc tccatgcata aatttaacag ctgaagcaag gaagaagatt
    30481 ccctctaaaa taccaaagaa aatcatcatc aataagacta aacctctttt tactcctttt
    30541 tgaattaaaa agaaagacag taaaatagtt gtcattagca tcgtaattgt tatcgctaat
    30601 ccatatgctg cttccatgtg cgctgaagtt ttaaaaagca aaacaataga ggttgtaatg
    30661 gcaaataaga accaattaat aacaggtatg taggtttgac caatattgtc accaggataa
    30721 gttgaacgaa attgtgggaa tatttttagt cgcatagctt cagaaactaa ggtaaatgat
    30781 ccagaaatca gtgcctgtga agcgatgata gctgccaaag tagctaaaat aacgacatgc
    30841 attgtaaatt gcgaaggaat actagcaaaa aagggattca attcatttcc tgcgttctta
    30901 ttagctaaaa tccatgcccc ttgcccacaa taagaaagta taatggcaac cttaacgaac
    30961 ggccatgaaa catgtatatt tccacgccca acatgaccta agtcagagta tagtgcttct
    31021 gctcccgttg tcgccaggaa aatagatcct aaaataaaaa tacctttatg attctctgga
    31081 ctaaataaca atttcaaacc gtagtatgga ttaattgctt tgaaaacttc tggatgggca
    31141 aaactattaa ggagaccgct aatacctaaa aaggcaaacc atataaacat aattggccca
    31201 aataatttcc caataacacc cgttccaaac ctttggatgg caaaaagtaa cagtaaaata
    31261 aacaaggtcg caaaaataac attactctga ttctggaaaa tatgctgcaa actaggaact
    31321 acttttaatc cttcaacggc tgaagttacg gttacagctg gcgtcaaagc tccatctgac
    31381 aacaaggttg cacctccaat aacagccgga acaattaacc aaggtgtcat ttttctaaca
    31441 agggtatata aagaaaaaat accaccttcg tggtgattat ccgcctttaa agctacaagc
    31501 acatacttga tagttgtaat aagtgtcaag gtccatatga ttaaagatat agaacctaag
    31561 ataaacgatt ctgtgacact agaaatacca ccttggtttt caaccaatga ttgcatcgta
    31621 tagagtgggc ttgtaccaat atctccataa acaatgccta aagcaataat aaatcctgct
    31681 tttgatgctt tatcaaaaga agaatgattg acatgttgca tttctgccaa ccctccgatt
    31741 aaataatatt agtcaaaaaa atggctgaac tcattatagc agattaagtt aaaaaaacaa
    31801 cacttatcta taaaaagacc agaaattatt aaagcgtttc ctggtcttta ctattttaat
    31861 cacgataaac aggttgaggt ccataagttt gactgactgg cattatttct atcgattaat
    31921 attaatgtgt tctggttggc tagcaaccca tgacacggta ttagcaatat cctctgcttg
    31981 aattgcatgc gcaccttcat aaagtttttc aactctttta tgatctcctt tgaaacgtac
    32041 agttgaaaat tctgtccctt cacataaacc gggttctagg ttagtaactc tgattttagt
    32101 tccagctaaa tcggcacgca agttgagagc aaattgtttt acaaaagctt ttgatgcgcc
    32161 atagacatta gctcctggat aaggaattgt tcctgcagtt gatcccaaat tgataattaa
    32221 ccctctatta acttctacca ttttcggtaa aatgcatcgt gtcaagtaaa ttaatcctac
    32281 aacgttagta ttaatcatag tcatccaatc ttcaaaatca gcttcatatg atttatccaa
    32341 tccaagtgct aaacctgcat tattaaccaa caaatcaatt gattgcaaat ttttaggcag
    32401 actttctaaa gctttgtcaa ccgattctct acttgtaata tccatttaaa gtggataaaa
    32461 gtaaccctct gaaaactttt ctccaaggca ttttaattta tccattcgac gagcacaacc
    32521 aataacgcta taaccatctg aaactaattt agtaacaaca gctgcaccaa aaccagcaga
    32581 tgcccccgtc acaagcgcaa ctttacgtga tatcataaat actcctcctc tagtatatag
    32641 aaaaacgagc taggaaaaat ccttctcgtt ttataactta ttttgatttc tcatcaatag
    32701 cttgagcagc tgtaatgata gcaagtttgt aaatatcttc agcacttgat ccacgtgata
    32761 aatcatttac tggagcattc aatccttgta agatcggtcc gattgcttca aacataccca
    32821 aacgttgtgc aattttatag ccaatatttc ctgattgaag gtctgggaaa ataaaaacat
    32881 ttgcttttcc agcaacatca ctattaggag ctttaatttc tgcagtttcc ggaacaaaag
    32941 ctgcatcaaa ttgtaattct ccatcaacag ctaattcggg agataaatct ttggcaattt
    33001 tagctgcttc ttgaactttt tcagcttgag gcgcttttgc acttcctttt gttgaaaaac
    33061 taagcatagc aatcttggga ttaatatcaa atattttggc tgtatccgca gtatttactg
    33121 caatttctgc tagctcttgt gcattaggat caatgttaat agcacagtca gcaaaaatat
    33181 aacgttcttg tgtattttca cggttcatca agaatacacc tgaagtgcgt gaaattcctg
    33241 gttttgtttt aataatttga agagcaggac gtacagtatc agctgttgaa tgaatggccc
    33301 ctgataccat accatctgcc aaaccaagtt ttactaacat aaccccaaaa tagttaacat
    33361 ccttcaaaag acgatctgca tcttccatag tagctttgcc tttacgaatc tcaacaaaag
    33421 cttctttcat ctcttcaaat ttttcgtatt gttcagggtc aataacgcta taatcttgat
    33481 caacaaaacc aagtcttgcc aaagaatcgt gaacttcgtc agccttacct aaaataacag
    33541 gatccacaag accttcaaat ttcaaacgag ctgctgcacg taatacacgt tcatcgcttc
    33601 cctcagggaa aacaatctta acataatcac ttgcaacttt ttctcttaaa ccaccaaata
    33661 aggaacgcat atatataaaa ccttctattt ctaaattaat gtaattccat gataacgctg
    33721 tcaaagtcat ctgtcaagtc tagcgtctgt ttatttattt ttcccataaa agggtcgtaa
    33781 aaagacaaag aatgacaatg caatgcttgc ctgtttattc ctagatccat acgtcctccg
    33841 tacaaatcat ctcccaaaag aggaaagccg atatgagcaa agtgaactct aatttgatga
    33901 gtacgccctg tatgtaattt gatatccact aatctaacat caccataacg agcaactact
    33961 tgataactag tatgtgcata tttgcctgat tcatggactc gcctcgtaat aatactatca
    34021 acatctcgtg caataggagc tataatatca ccactatctg ctaaatcacc actccctgat
    34081 actaatgcat aatacctttt ttcaattgct ttagcctgaa gttgtttatc tagacgagca
    34141 tgggcataac catgcttggc gaataacatt aatccacttg tatctcgatc caaccgggtc
    34201 acaatatgga cctgctgatt ggcatagtta ttagacacat aatagtgttt tataaaatta
    34261 gcaattgtat ttgaatgaat agagctagga atactagcga aaccaaaagg tttatttaaa
    34321 atcaaaaaat gatcatcctc ataaacaata tctaaatcgt gaggtactgg ttccaaagtt
    34381 tcgtgagaag gctcgtcagg aatatctatc gtaacaacat cgccaatctc taataagaaa
    34441 atagcgttct gctcttcacc attgacaaaa atcttaccac ctctgtactt aatctttgct
    34501 aataaccccc ttgaaacatc atgacttttc aaaagtgttt taactttgca cctttcttct
    34561 gctatgtatt caaatttcaa tgttcaggtt ctccgataaa agcatcgtta acacgttccc
    34621 aaaagctagt gtgacttgga gttgaaacaa agttaatact tttttcgtca atactatatt
    34681 ctattttggt aacatttttg taatggaccg ttttgttatc tatagaaatt gtatagacac
    34741 caactcgttt aggaactatc tctatagcat cttttttggg gatgataacc gatgacccca
    34801 aggtacgata gacgcgatta ttaagactag aaatttctgt tagttgcaag gcttctatcg
    34861 ttgggtggag tactgccccc cctagtgatt tattgtaagc agtactaccg gttggtgtag
    34921 aaactaaaat gccatctccc ctaaaccttt caaataccac ttgattaata acaacatccg
    34981 ctaccattgt tttttcaata cgcttaatag tagactcatt taaagcacgc gcacgaataa
    35041 ctcgaccatc ttcaagggta atagttactt tcaaaatagg ataggagatt tgttcacctt
    35101 tatcattttt caaattatta attaatgtgt caacttcaaa atctcgatag tctgtataaa
    35161 accctaaatg tcctgtatga acaccgacaa accttacttt atccagttgt ttttcgtaca
    35221 tatggaaggc tgacaaaagc ataccatctc caccaataga aatgacaata tcaggatcct
    35281 tttttgataa ataaaaatca ggatcatgtt taaaagcagc aaagagcttt gaggctacac
    35341 gcttgctttg atattttccg ttagcaatta tagcaacacg tgttgctcta tctgtaaaat
    35401 tcatctgtgt catcactatt tcctacacca tcgctcaatt ttcgatttaa aggatcaaat
    35461 aaaagctgtg cttcacgaat atcttcgcga atcttacgca tttcttcatc caattctaaa
    35521 gcgatttttg ctgttttctc cagtctttgt ttaatttctt cagggaagtc cccctgatac
    35581 ttgtaattta atgaatgttc gatggttgcc caaaaattca tagccagagt tctaatttgt
    35641 atttctgcta aaactttctt ttgtccatct attgtgtcta caggatactc tacaacgaca
    35701 tgatatgaac gatatccact agatttcata tgggtaatat agtctcgctc ctgaaccact
    35761 gtcatgtcat gacgttttct caacaaggca aggacttcat caacgtcatc tacaaattgt
    35821 accatgatac gaagacctgc aatatcttgc aagtcctgag caagattttc ttctgaaata
    35881 ccacgtaaaa ccatcttttc ttgaatactt tcaaccgact ttacgcgtcc agtaacaaat
    35941 tctattggag agtgacgatt ttgctttcta aattgtttac gaataccacg aagctttatt
    36001 tttaattcac caaccgtttg aatataaggg tctagaaatg tttcccaatc cattgacatt
    36061 ctaatcctcc aaaatattta atgtactctc ttgtaaaata aacttttttt ctataaaatt
    36121 aacacaacaa ttaatattat accatattta ggaaaaggaa gtgataagat gacacaccta
    36181 gaaattgaat acaaaactct attgaataaa gacgaattta atcgtctaac aagcttattt
    36241 tctcatgttc aacctattac tcaaaccaat tactattttg acacagaaac atttgaaatg
    36301 aaagcacacc gaatgtcact tcgcattaga acgttaccta accgtgccga actaacacta
    36361 aaaatcccta gagaagtagg aaatttagag cacaatcatg accttacttt agaagaagct
    36421 aaatatatcg ttaaaaatgg ccagttccct gaagatactg agattgcttc tttaatcctt
    36481 gaaaagggag ttgatccaac caaattagct gtctttggac aacttacaac aacaagacga
    36541 gagatggaaa cttcaatcgg cttaatggct ctagacagca atatatatgc tgatattaaa
    36601 gattatgaac ttgagttaga agtcaaacaa cctaaacaag ggaaaagaga tttcgaccag
    36661 tttttaaaag aaaataatat taattttaaa tacgccaaaa gtaaggtggc tagattctct
    36721 gctactttaa aaaataacta acgttgagag attggatttt ccaatctctt ttataattct
    36781 catttaagca agaacttaaa atctcatact atctctcctc aacattgtta ttgcaagcat
    36841 tttcatgttt cttatcgtga attttttcta tttttttggt aaactattag tatcaatcta
    36901 aaggagtcat ctatctcatg gcagaacaat acgccgataa acaaatcaaa cttttctcat
    36961 taaccgctaa tagagaaatt gctgaaaaaa tttcccaagc ttcaggaatt cctttgggta
    37021 agatgtcatc acgtcaattc tctgatggcg aaattatgat taatattgag gaaacggtgc
    37081 gcggtgacga tatctatatc atccaatcta caagcttccc agtcaatgat aatctctggg
    37141 aattactcat tatgattgac gcttgcaaac gtgcaagtgc taatactgtt aacatcgtag
    37201 tcccttattt tggatactct cgtcaggacc gcatcgctgc atctcgtgaa cctattactg
    37261 caaaattggt cgctaatatg ttagttaaag ctggagttga tcgagttctt accctcgact
    37321 tacatgcagt gcaagtacaa gggttcttcg atatcccagt tgacaatctt tttactgtcc
    37381 cactatttgc tgaacactat aatcaactcg gtctctctgg tgaagatgtc gtcgttgtta
    37441 gtcctaaaaa ttctggaatc aagcgtgctc gtagtttggc agaatacctt gactctccaa
    37501 ttgctatcat tgattatgca caagatgatt ctgaacgtga agaaggctat attattggtg
    37561 aggtagaagg taagaaagct attattattg atgatatttt aaatacagga aaaacctttg
    37621 ccgaagctgc aaaaatttta gaacgtggtg gagcaactga gatttatgct gttgctagtc
    37681 atgggctttt tgctggcggc gcagctgata ttttagagtc tgcaccaatt agagaaatta
    37741 tcgtaacaga ctctgtttta tctaaagagc gtattcctag caatattaaa tatctgaccg
    37801 ctagccatct cattgcagac gctattatcc gtatccatga aagaaaacct ttaagtccat
    37861 tattttcata tcgttcagat aagaaggatt aattgatgat ttatttagac aatgctgcta
    37921 ctaccgctct aaccccatct gttattgaga aaatgaccaa tgtcatgaca agtaactatg
    37981 gtaatccatc tagtatacat acctttgggc gtcaagcaaa tcaactttta cgtgaatgtc
    38041 gacaaattat tgctgaatat ctaaatgtta attcacgtga aattattttc acttctgggg
    38101 gaactgagag caacaataca gctatcaaag ggtatgctct tgcaaatcag ctaaaaggta
    38161 aacatattat tacctctgaa attgaacatc attcagtcct acatactatg acttacttat
    38221 cagagcgatt tggttttgat attacttact taaaaccaaa ccatggacaa attactgcaa
    38281 aagacgttca agaagcttta cgagatgata ctattatggt atctctcatg tttgctaata
    38341 atgaaaccgg ggacttttta ccaattcaag agattggtca gcttctcagg aaccaccaag
    38401 ctgtttttca cgttgatgcc gttcaagtct ttagcaaaat ggaacttgat cctcattctt
    38461 taggaattga ctttttagct gcttctgccc ataaatttca cggtccaaaa ggtgttggga
    38521 tactttactg tgctccccat cactttgata gtctacttca tggtggagac caagaggaaa
    38581 aaaggcgtgc ttcaactgaa aatataattg gtattgctgg aatgtctcaa gctcttactg
    38641 atgctacgac taacaccctt aaaaattgga ctcacattag tcagctgaga acgacctttt
    38701 tagatgctat ttcagacctt gacttctatc ttaataacgg tcaagactgc ttacctcatg
    38761 tacttaatat aggttttcct agacagaata atggcttgtt attgacacag ttagatttag
    38821 ctggattcgc agtttcaaca ggttctgcat gtactgcagg aacagtcgaa cctagtcatg
    38881 tcttaacaag cttgtatgga gccaactcac cacgtctaaa tgaatcaata cgtattagtt
    38941 tttcagaact aaatacccaa gaagaaattc ttgaattagc taaaacctta agaaaaatta
    39001 taggagatta atatggcatt tgaaaaagaa ataaatcttc cagactgcaa ttacatttac
    39061 actatcagcc cgaacattaa aaaatatacc ttacgcgata ctacattcac tcaaaatcgc
    39121 ttaggtaatt atgaattaga gcgtctccta gaaacagtac ctaattctgg cgatggcttt
    39181 cctctaaaga ttacaatcaa taaagaactt tctggtttta agctcttaat tactgataaa
    39241 tctggcctta gaatagtaaa tatctttaaa tctaccgatc ataaaattct tcaagacaaa
    39301 ttttatttct taatggacag cctcgttgag cgtaatatct ttacgaaaac tgcaaaataa
    39361 tttaccgacc actattttta acattttagt ggtctttttg cgaaccattt tgttgtcact
    39421 ttcacaatct ttttgtacaa tagagatgtg aagaattaag ttatataact gattctaaat
    39481 cgaataataa aatttttaac aaggagataa ttttgtgatt atggataagt ctattcctaa
    39541 agcaactgct aaacgtttat cactgtacta ccgtattttt aaacgtttta atactgatgg
    39601 catcgaaaaa gctagttcca aacaaattgc agatgcccta ggtatcgatt ctgctactgt
    39661 tcgacgtgat ttttcttatt ttggtgaact aggacgccgt ggttttggtt atgatgtcaa
    39721 aaaacttatg aacttctttg cagaaatatt gaacgatcat tctacaacaa atgttatgct
    39781 ggtggggtgt ggaaatatcg gtagagctct cttgcattat cgtttccacg atcgcaataa
    39841 aatgcaaatt tcaatggctt ttgatttaga tagcaatgat ttagttggta aaacaaccga
    39901 ggatggaatt cctgtctacg gtatttcgac tatcaatgac catttaatag atagtgatat
    39961 tgaaactgct atcctaacag tacctagtac agaagcccaa gaagttgctg acatcttagt
    40021 caaagcaggt ataaaaggca tcttgagttt ttctccagtt catttaacat taccaaaaga
    40081 tatcattgtt cagtatgtag atttaacaag cgaattacaa actttacttt atttcatgaa
    40141 ccagcagcga taaatagttc attatttata aaatatctgc ttcttctcga aaactataat
    40201 atttattttt tccaacgatg atgtgatcta ggcagacaat tcccagatga tcacatgatc
    40261 gttttatttt tttagtgaaa cttaaatcac tttcactggg atttggagaa cctgagggat
    40321 gattatgtat aataatcaaa gaagttgcca tgtttttaca agcataatgt agaatttctc
    40381 ttggctctgc tactgaacga cgtacagtac caataaaaat agttctctgt tcgataatac
    40441 gattttgtgt atccatataa atagctacta aatgttcttg ttttttatcc cctaattcga
    40501 gcatcatttt cctcgctaat tgttcactac ttaaaatttg ctctttacga tcatattcag
    40561 ctttgtgaat ccgttttgct agttctagca tagcttttat ttcgacggat ttaacctgac
    40621 cgattccttt aatggattgc aactcctgta aggataattg accaaaatct gctaaactgc
    40681 ttatgttttc taaaatttgc gttgaaattt caagaacagg tttttcttta atacctgtac
    40741 gtaagagaat ggctaataac tcctgattac tcaatctatc tgcgcctaaa tcaactaggc
    40801 gttctcttgg tagtaaagct tcctttttta attcaatatg gtacataaaa cacctccagt
    40861 cataatattc gtaaactaat caaaaactca tgttttaaat caataaaaaa tacttagatg
    40921 aaaaatctaa gtatttttta tttaacgata ggttcgtgca cgatttctaa gcgtttgtcg
    40981 cttgataata tgatctttta caatctgata gatagtagct gcaacaggaa cagcaagtaa
    41041 catgcctaag atcccccata aagcacctcc gatagtaata gccattaaaa cccacataga
    41101 aggcagtcca atcgatccac cgacaacttt cggataaatg acatttccct caaattgttg
    41161 taaaaggatc aagaaaagaa caaacaagaa tgcttcagta agcgattcag tagcaattaa
    41221 gatgaaaccg attgtaacac caatgtaggc tcccacaacc ggtattagag ctgtaaaagc
    41281 aactaaaacc ccaactgtta aagcataagg aaattggaag atcaacatac caataaccgt
    41341 caaacttcct aaaatcatag cttctaaagt ttggcttaca aaaaaaccat ggaaacgttg
    41401 atgaaggata tgacgaacgt aatggaatgt tttgcctgtt gaacctaaat aggtatcaat
    41461 taacaaatta aattgacgtc ccaactgctc cttgtttgcc aaaacgtaaa ttgaaaaaat
    41521 aaaactaaca aaaacattca gaagtgttgc cgcaatagag gaaactgagg ttagtaaatt
    41581 tgttaaaaca gataaaactt gcttcaaaat ctgctggcta taaccactta aagtagaaac
    41641 taagtcttta tctgttccca tataatttaa agcctcagaa atttgtttat tttcattgag
    41701 attattaacc aatttagcaa gtgctccggt atcaataacc aataaagaac tcaaactaga
    41761 aatcaaatct ggaatgacaa ttgaaaatag ccagacaatt aaaccaataa aagttgcata
    41821 ggataaaatc atagaaacac t
//
LOCUS       DATROQ010000009        41788 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.009, whole genome shotgun sequence.
ACCESSION   DATROQ010000009 DATROQ010000000
VERSION     DATROQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41788
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.009"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            33..575
                     /locus_tag="VBC94_000418"
     CDS             33..575
                     /locus_tag="VBC94_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEO6978717.1"
                     /translation="MSIIIGLMAAMFIIRLAYLKLSIANEKALRKNGAKEYGVGVSKA
                     ITVLHIIIYFSSVTEAVLTKASFNFVSVIGLSLMIFSVFMLHTVTRLLGRIWTVKLMV
                     DKNHQFVDHWLFRVVKHPNYFLNIAPELLGVTLLCHAKYTALFVLPIYAFVIYLRIRE
                     ENLLLKTIIIPNGIKKSRVS"
     gene            888..1802
                     /gene="glyQ"
                     /locus_tag="VBC94_000419"
     CDS             888..1802
                     /gene="glyQ"
                     /locus_tag="VBC94_000419"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEO6978718.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLKSLEL
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLQTGPVTSE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFTQPEYEHSKYSFEISDQVMLLENFE
                     KFEREAKRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEETRIKLLAEED"
     gene            1802..2443
                     /locus_tag="VBC94_000420"
     CDS             1802..2443
                     /locus_tag="VBC94_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6978719.1"
                     /translation="MNTLIVNSHPDFSNPYSFTTILQEKFIELYNEHFPNHQLSILNL
                     YDCVLPEITKEVLLSIWSKQRKGLELTADEIVQAKISKDLLEQFKSHHRIVFVSPMHN
                     YNVTARAKTYIDNIFIAGETFKYTENGSVGLMTDDYRLLMLESAGSIYSKGQYSPYEF
                     PVHYLKAIFKDFMAFDDFCVVRAEGTDILDRQVVLDKMNQDLREAFEAFYSKE"
     gene            2447..4486
                     /locus_tag="VBC94_000421"
     CDS             2447..4486
                     /locus_tag="VBC94_000421"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEO6978720.1"
                     /translation="MTKDLLLELGLEELPAYVVTPSEKQLGQKMVKFLEDHRLSFETV
                     QTFSTPRRLAVRVKGLADQQTDLTEDFKGPSKKIALDAEGNFSKAAQGFVRGKGLSVD
                     DIEFREVKGEEYVYVTKHETGKSAIDVLASVTEVLTELTFPVNMHWANNSFEYIRPVH
                     TLVVLLDDQALELDFLDIHSGRISRGHRFLGSDTEISSASSYEDDLRQQFVIADAKER
                     QQMIVNQIHAIEEKKNISVEIDEDLLNEVLNLVEYPTAFLGSFDEKYLDVPEEVLVTS
                     MKNHQRYFVVRDRDGKLLPNFISVRNGNAEHIENVIKGNEKVLVARLEDGEFFWQEDQ
                     KLNIADLVEKLKQVTFHEKIGSLYEHMDRVKVISQHLADKAGLSDEEKLAVLRAASIY
                     KFDLLTGMVDEFDELQGIMGEKYALLAGEQPAVAAAIREHYMPTSADGELPETRIGAI
                     LALADKFDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILEAFGWDIPLDELVTNLYG
                     LSFASLDYANQKEVMAFISARIEKMIGSKVPKDIREAVLESDTYIVSLILEASQALVQ
                     KSKDAQYKVSVESLSRAFNLAEKVTHSVSVDSSLFENNQEKALYQAILSLELTEDMHD
                     NLDKLFALSPIINDFFDNTMVMTDDEKMKQNRLALLNSLVAKARTVAAFNLLNTK"
     gene            4498..4755
                     /locus_tag="VBC94_000422"
     CDS             4498..4755
                     /locus_tag="VBC94_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEO6978721.1"
                     /translation="MDPKKIARINELSKKKKTVGLTGEEKVEQAKLREEYIEGFRRSV
                     RHHVEGIKLVDDEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            4844..5107
                     /locus_tag="VBC94_000423"
     CDS             4844..5107
                     /locus_tag="VBC94_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEO6978722.1"
                     /translation="MIELLLALLLWNILVFATYAIDKRRAIRGAWRISEKTLLIESLL
                     LGGFGAFLGGEVFRHKTTKWYFRLVWYIGMIIDLAIIWLIWCY"
     gene            5222..6730
                     /gene="glpK"
                     /locus_tag="VBC94_000424"
     CDS             5222..6730
                     /gene="glpK"
                     /locus_tag="VBC94_000424"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEO6978723.1"
                     /translation="MSSEEKYVMAIDQGTTSSRAIIFNKKGEKIASSQKEFPQIFPQA
                     GWVEHNANQIWNSVQSVIAGAFIESSIKPGQIEAIGITNQRETTVVWDKKTGLPIYNA
                     IVWQSRQTAPIADQLKQEGHTNMIHEKTGLVIDAYFSATKVRWILDHVPGAQERAEKG
                     ELLFGTIDTWLVWKLTDGLVHVTDYSNAARTMLYNIKELKWDDEILELLNIPKAMLPE
                     VKSNSEVYGKTTPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMN
                     TGEEMQLSQNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLRMIETSSESEGL
                     AQSSTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRD
                     VIDTMQVDSGIDIQQLRVDGGAAMNNLLMQFQADILGIDIARAKNLETTALGAAFLAG
                     LSVGYWESMDELKELNATGQLFQATMNESRKEKLYKGWRKAVKATQVFAQED"
     gene            6743..8572
                     /gene="glpO"
                     /locus_tag="VBC94_000425"
     CDS             6743..8572
                     /gene="glpO"
                     /locus_tag="VBC94_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEO6978724.1"
                     /translation="MEFSRETRRLALQRMQDRTLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVTNTPAANKVLSAEDVLKREPDLQKEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGAYIASHVKAEDFLFDDNNQIIGVRARDLLT
                     DQVIDIKARLVINTTGPWSDTVRNFSNEGKQIHQLRPTKGVHLVVDRQKLNVSQPVYV
                     DTGLNDGRMIFVLPREDKTYFGTTDTDYHGDLEHPTVTKEDVDYLLNIVNKRFPEAEL
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKLSDESFEELIDSVKDYIAHKNHREDVE
                     KAISHVESSTSEKELDPSAVSRGSSFERDDNGLLTLAGGKITDYRKMAEGAMETIINI
                     LDKEYNRKFKLINSKTYPVSGGEINPSNVDSEIEAYAQLGTLSGLSIEDARYIANLYG
                     SNAPKLFALTRQITEAEGLSLVETLSLHYAMDYEMALSPTDFFLRRTNHMLFMRDNLD
                     SLIQPVIDEMAKHYQWSDQDKTFYEEELHETLKDNDLAALKDN"
     gene            8584..9282
                     /locus_tag="VBC94_000426"
     CDS             8584..9282
                     /locus_tag="VBC94_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6978725.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKNHNSGWIVITFGWG
                     LAVAIAALVSGNISPAHLNPAVSLAFAIKGDLAWGTAILYMIAQIIGAMLGSLLVYLQ
                     FRPHYEAAENKADILGTFATGPALKDNFSNFLSEVLGTLVLVLTIFAIGKYNMPPGVG
                     TMSVGMLVVGIGLSLGGTTGYAINPARDFGPRLLHALLPMKNKGDSDWTYSWIPIVGP
                     MVGAILAALIFAMM"
     gene            9370..10707
                     /locus_tag="VBC94_000427"
     CDS             9370..10707
                     /locus_tag="VBC94_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6978726.1"
                     /translation="MTEKYVNKVIVILGASFAGMTCAQKLRQLNPNWDIVLIDKEIHP
                     DYVPNGLNWYYRHEISGLNQAMWQTEEEQRLQNIRCLFGLKVEKINKEDRELMLSDGS
                     SVYYDQLICAMGSQAESTYIDGADAQGVLTTKTYATSQNAKQVLDKSHKVAVVGAGII
                     GLDIAYSLHESGKAVTLLEAQERPDFRHTDPDMSLPLLDAMAESKLHFFQNQKVEKIT
                     VTREEKLCLRTLTGDTFTVDTVILAVNFRPDSRLLTGLVDLSVDNSVVVNDYFQTSDP
                     NIYAIGDLIWSYFKGLNSAYYMPLINQAIRSAQMLAYHLSGHAVPKLKITRATGSKHF
                     GYYRANIGLTELEAGLYEDTVSVTYFPKEQYDLRIKLIANQKTGHLLGAQLISKENCL
                     ATANQLVQAISCDMTDFDLAFQDFIYTARESEMAYMLHQAAINLYEKRIGLCE"
     gene            10698..12128
                     /locus_tag="VBC94_000428"
     CDS             10698..12128
                     /locus_tag="VBC94_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6978727.1"
                     /translation="MRIEALMEKERRVQYRLLSFLRGSPQAIALKLALLETGLSRATL
                     LKYINNLNSYFEQEKVNCRIVYYKDKLFLEEDYNLSNQEVLKALMKDSIKYTILISLF
                     NQRQFTIVGLSQELMVSEATLNRHLAHLNELLAEFDIAISQGKQIGDELQWRYFYYEL
                     FKQLWSYDKCQNMIKKLDLDSLILLIERLAQHTLTREAQQNLGLWFSICHHRLLAMEK
                     LSDNLKPIVEHYQCNAFYKRLDAALVLYMSRFALEYREGEVLATFAFLHSQNILPINT
                     MEYIMGFGGPIIDCVTETIIYFKKESILADETSDQVIYQLGQLYSHYYFFKGHILVEQ
                     PDLEQTYRLIDHNMRDKLHDISKKIIANVNRIRPLTEDGCSLLTLHLLELLIFSKNSQ
                     KMPFRIGLDMTGNAVEQSLLEYRIRQHFSGNNSIQVEPYDEGKGFDMVIYQSRSRPYK
                     AKLTYCLNKGASERELQEIDSLIYDN"
     gene            12253..14238
                     /gene="tkt"
                     /locus_tag="VBC94_000429"
     CDS             12253..14238
                     /gene="tkt"
                     /locus_tag="VBC94_000429"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO6978728.1"
                     /translation="MTFDTIDQLAVNTVRTLSIDAIQAANSGHPGLPMGAAPMAYVLW
                     NKFLNVNPKTSRNWTNRDRFVLSAGHGSALLYSLLHLAGYDLSIDDLKQFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDLVDHYTYTLHGDG
                     CLMEGVSQEAASLAGHLKLGKLVLLYDSNDISLDGPTSQSFTEDVKGRFESYGWQHIL
                     VKDGNDLEAIAAAIEAAKAETDKPTIIEVKTIIGFGAEKQGTSSVHGAPLGAEGITFA
                     KKAYGWEYPDFTVPAEVAARFASDLQARGAKAEEAWNDLFAKYEVEYPELAAEYKEAF
                     AGQAETVELKAHDLGSSVASRVSSQQAIQQLSTQLPNLWGGSADLSASNNTMVAAETD
                     FQASNYAGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWQRAVSET
                     DRPTMLVLTRQNLPVLEGTSELAQEGVNKGAYILSEAKGELDGIIIATGSEVKLALDT
                     QDKLESEGIHVRVVSMPAQNIFDEQEASYQEQVLPSAVTKRLAIEAGSSFGWGKYVGL
                     NGLTLTIDTWGASAPGNRIFEEYGFTVENAVSLYKEL"
     gene            14450..14755
                     /locus_tag="VBC94_000430"
     CDS             14450..14755
                     /locus_tag="VBC94_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO6978729.1"
                     /translation="MKKECRDFYRQIQHTYNDISVREDAVLSSILLSASNGLIKTSDV
                     PRVAYELTQQLENNEIEKSFESLATVKELKKSAKKLSERYEQNKVTTGLLSKVFGNH"
     gene            14775..15509
                     /locus_tag="VBC94_000431"
     CDS             14775..15509
                     /locus_tag="VBC94_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6978730.1"
                     /translation="MIKFEHVSKVYGEKEALSDLTLSIKDGEIFGLIGHNGAGKTTTI
                     SILTSIIDATYGQVYIDDLLLTEHRDQIKKKIGYVPDSPDIFLNLTAEEYWYFLAKIY
                     DVAPEDIEARITKLVDIFELEEQRYNPIESFSHGMRQKVIVIGALLPNPDIWILDEPL
                     TGLDPQASFDLKEMMKEHAKNGKTVIFSTHVLAVAEQLCDRIGILKQGKLIFVGSLGE
                     LKMQYPDKDLETIYLELAGRQANREG"
     gene            15513..17117
                     /locus_tag="VBC94_000432"
     CDS             15513..17117
                     /locus_tag="VBC94_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6978731.1"
                     /translation="MNWSRIWELVKINILYSNPQTLSALRKKQEKHPKKEFSAYKSMF
                     RNQLFQILLFSIIYVFLFVSLDFKEYPGYFTFYIGIFTLVSIIYSFIAMYSVFYESDD
                     VKQYAYLPIKSEELYVAKIFATFGMSVTFLMPILTLMIVAYWRIIGGPLAVLLAIINF
                     AILFLSVTVISLYINSLIGRAIIRSANRKLISTILISLATFGAIVPLLFVNMTSQKMV
                     QGKLQDIAPIPYVRGYYDIVTAPFSMESLLNYYLPLLIILFLIGAIYKWVMPRYYQEL
                     LYGQVKQRKVHRQIDFSKRESINKTLVKHHLSSLQNATLLTNTFLMPLLYLAMFIVPI
                     LNNGKEIGRFFNENYFGIAFLAGILIGSLCVMPASIVGVGISLEKSNFYFIKSLPISF
                     SYFLKHKFVTLITLQLAVPTFIYFLVGFFLLKLSILVLLSFILGLVFMGLIEGQFIYR
                     RDYKHLFLNWQEVTQLFNRGLGQWLLVGSLFGMMIIGSFLIGISIFWSMVWNTVAVNI
                     IILIIGLLILSICQYLLLKNFWKKLV"
     gene            complement(17325..18711)
                     /locus_tag="VBC94_000433"
                     /pseudo
     CDS             complement(17325..18711)
                     /locus_tag="VBC94_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643962.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            18852..19655
                     /gene="proB"
                     /locus_tag="VBC94_000434"
     CDS             18852..19655
                     /gene="proB"
                     /locus_tag="VBC94_000434"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEO6978732.1"
                     /translation="MKRHFETTRRIVIKVGTSSLVQTSGKINLSKIDHLAFVISSLMN
                     KGMEVILVSSGAMGFGLDILKMDKRPQEISQQQAVSSVGQVAMMSLYSQIFSHYQTHV
                     SQILLTRDVVVFPESLQNVTNSFESLLSMGILPIVNENDAVSVDEMDHKTKFGDNDRL
                     SAVVAKITKADLLIMLSDIDGLFDKNPNIYDDAVLRSHVSEITDDIIKSAGGAGSKFG
                     TGGMLSKIKSAQMVFDNNGQMILMNGANPRDILKVLDGHNIGTYFAQGK"
     gene            19665..20918
                     /locus_tag="VBC94_000435"
     CDS             19665..20918
                     /locus_tag="VBC94_000435"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEO6978733.1"
                     /translation="MTYIEILGQNAKKASQSVARLSTASKNEILRDLARNIVADTETI
                     LTENARDVAKAKDNGISEIMVDRLRLNKDRIQAIANGIYQVADLADPIGQVVSGYTNL
                     DGLKILKKRVPLGVIAMIFESRPNVSVDAFSLAFKTGNAIILRGGKDAIFSNTALVNC
                     MRQTLQDTGHNPDIVQLVEDTSHVVAEELMQATDYVDVLIPRGGAKLIQTVKEKSKIP
                     VIETGVGNVHIYIDEFADLDMAAKIVINAKTQRPSVCNAAEGLVVHQAIAKGFLSQLE
                     KMLKESNQSVEFRADEEALQLLENAVAASETDYATEFLDYIMSVKVVDSFEQAISWIN
                     KYSSHHSEAIITNNISRAEIFQDMVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKLH
                     ARGPMGLEALTSTKYYINGTGQVRE"
     gene            21001..21948
                     /gene="rsmH"
                     /locus_tag="VBC94_000436"
     CDS             21001..21948
                     /gene="rsmH"
                     /locus_tag="VBC94_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEO6978734.1"
                     /translation="MTNDFHHITVLLHETVDMLDIKPDGTYVDATLGGAGHSEYLLSQ
                     LGPDGHLYAFDQDQKAIDIAHIRLKKYVDTGQVTFIKDNFRNLSSNLKALGVSEINGI
                     CYDLGVSSPQLDERERGFSYKQDAPLDMRMNREQSLTAYDVVNTYSYHDLVRIFFKYG
                     EDKFSKQIARKIEQVRAEKPISTTTELAEIIKSSKSAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDLLAVDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLQPKMELVNRKPILPSQEELEANNRAHSAKLRVARRIR"
     gene            21963..22289
                     /gene="ftsL"
                     /locus_tag="VBC94_000437"
     CDS             21963..22289
                     /gene="ftsL"
                     /locus_tag="VBC94_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEO6978735.1"
                     /translation="MTNEKRTEAVTQTLQRHIKTFSRIEKAFYGAIVITAIIMAVGII
                     YLQSNSLQVKQEVNQLNSKINDKQTEFDNAKQEVNELSNRDRITKIAKDAGLTIQNDN
                     IYRKVD"
     gene            22293..24551
                     /locus_tag="VBC94_000438"
     CDS             22293..24551
                     /locus_tag="VBC94_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6978736.1"
                     /translation="MTFFKKLKKIFLDYVIHIRDRRSPQKNRERVGQNLMILTIFLFF
                     IFIINFVIIVGTDSKFGVNLSKEAKKVYQQSMTVQAKRGTIYDRNGNPIAEDATTYSL
                     YAIISKNYTTATGQKLYVQPSQYEKVASILENKLGMKKNLVLKQLNQKKLFQVSFGSS
                     GSGLSYTKMADIKKTMEKSDIKGIGFSTSPGRIYPNGIFASQFIGFTLPQDDGDGKKL
                     VGNTGLEAALNKVLSGTDGKVTYEKDRSGNVLLGTATTERRAVNGKDIYTTLSEPIQT
                     VLETQMDVFAEKTKGKFASATVVNAKTGEILATSQRPTYNPSTLKGYDKKNLGTYNTL
                     LYDNFFEPGSTMKVMTLASAIDSKHFNSTEVYNSAQYKIADAVIRDWDVNEGLSSGSY
                     MTFPQGFAHSSNVGMVTLEQKMGRDKWLNYLSKFKFGYPTRFGMLHESGGLFPSDNEV
                     TIAMSSFGQGIGVTQVQMLRAFTSISNDGVMLQPQFISSIYDPNTGTSRTARKEVVGK
                     PVSKEAASKTRDYMVTVGTDPYYGTLYAAGAPVIQVGNQSVAVKSGTAQIAQEGGGGY
                     LQGKNDTINSVVAMVPSENPDFIMYVTIQQPEKFSITFWKDVVNPVLEQATAMKETIL
                     KPVLNDSEHQTKYKLSKIVGENPGHVAEELRRNLVQPIILGNGSKVSKVSKRPGANLA
                     ENEQLLVLTNKLTELPDMYGWSKANVEQFAKWTGIKVTYKGSTSGKVRKQSIDVGKSI
                     NKIKKIKITIGD"
     gene            24553..25563
                     /locus_tag="VBC94_000439"
     CDS             24553..25563
                     /locus_tag="VBC94_000439"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEO6978737.1"
                     /translation="MFLSIMAGVIAFVLTVIAIPRFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLVVALLVSLIFSIILSKENSGNLGATFGILSVVLIYGIIGFLDDF
                     LKIFKQINEGLTPKQKMSLQLIAGLIFYFVHVLPSGTSAINIFGFNLEVGYLYAFFVL
                     FWVVGFSNAVNLTDGIDGLASISVVISLITYGIIAYNQTQFDILLIIVIMIGALLGFF
                     VFNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLFIGFVYVFETSSVMLQVAYFK
                     YTKKKTGVGKRIFRMTPFHHHLELGGVSGKGNKWSEWKVDAFLWAIGIFMSAITLAIL
                     YL"
     gene            25661..27004
                     /locus_tag="VBC94_000440"
     CDS             25661..27004
                     /locus_tag="VBC94_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6978738.1"
                     /translation="MSFKDFNFKPYIQRALDELKFVDPTDVQAKLIPVVRSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDESSDDVQVVITAPSRELATQIYQATKQIAEHSEQEIRV
                     VNYVGGTDKLRQIEKLKVSQPHIVIGTPGRIYDLVKSGDLVIHKAHTFVVDEADMTLD
                     MGFLDTVDKIAGSLPKDVQILVFSATIPQKLQPFLKKYLTNPVMEKIKTATVIADTID
                     NWLLSTKGRDKNAQILELSKLMQPYLAMIFVNTKERADELHSYLSSNGLKVAKIHGGI
                     APRERKRIMNQVKNLEFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLSGTAITLYQPSDDSDIRELEKLGINFIPKVIKNGEFQDTYDRDRRNNREKSYQK
                     LDTEMIGLVKKKKKKIKPGYKKKIQWKVEEKRRKERRASNRAKGRAERKAKKQSF"
     gene            27142..27954
                     /locus_tag="VBC94_000441"
     CDS             27142..27954
                     /locus_tag="VBC94_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6978739.1"
                     /translation="MFKKIILSIATIAATASLAVSVQASEKVELKVATDSDTAPFTYQ
                     KDGKFKGYDVDVVKAVFKGSKYKVTFKTVPFDTISTGIDAGKFDLSANDFSYNKERAE
                     KYLFSDPISRSNYAVVGKKGSHYKSLSDLSGKSTEVLSGVNYAQVLENWNKNHPNKKP
                     IKIKYVSGTTGVTSRLKNIESGKIDFILYDAISSDYIVKDQSLNLSVSPLKGKIGNNK
                     DGLEYLLLPKDKKGKTLQKFINKRIKVLKEDGTLARLSKQYFGGDYVSNIDK"
     gene            28049..28852
                     /locus_tag="VBC94_000442"
     CDS             28049..28852
                     /locus_tag="VBC94_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6978740.1"
                     /translation="MNQFILTGGWSWYNNLVSQVPAGKLFSWKAVLDAIPSILERLPI
                     TLLLTVAGALFGLILALIFAVVKINRVKILYPIQALFVSFLRGTPILVQLMLSYYGIP
                     LFLKFLNQKYGFDWNINAIPASVFAITAFAFNEAAYTSETIRAAILSVDQGEIEAARS
                     LGMTSAQVYRRVIIPNAAVVATPTLINTLIGLTKGTSLAFNAGIVEMFAQAQIMDGSD
                     YRYFERYISVALVYWAVSFLIEQLGNAIERKMAIKAPHHLTDEIPGGVR"
     gene            28852..29595
                     /locus_tag="VBC94_000443"
     CDS             28852..29595
                     /locus_tag="VBC94_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6978741.1"
                     /translation="MIKLRQLTKSFSGQKVLDKLDLDIEKGQVVALVGASGAGKSTFL
                     RSMNYLEEPDYGTIEIDDFKVDFKSISKDDILTLRRKLAMVFQQFNLFERRTALDNVK
                     EGLKIVKKMSDQEATRIARDELAKVGLADREKYYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKQGQTMVLVSHDMNFVYQVADKVLFLEKGRIL
                     ESGTPEQLFNHPLEERTKEFFASYNKSYL"
     gene            29717..29941
                     /locus_tag="VBC94_000444"
     CDS             29717..29941
                     /locus_tag="VBC94_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEO6978742.1"
                     /translation="MFLEIAKVYLQGLGLASITVILVGIFWVFWRAKKNIDKTARERQ
                     SVLYDVLLIALMTAPILAFAFVAIILMIKA"
     gene            30010..30924
                     /gene="trxB"
                     /locus_tag="VBC94_000445"
     CDS             30010..30924
                     /gene="trxB"
                     /locus_tag="VBC94_000445"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEO6978743.1"
                     /translation="MYDTLIIGSGPGGMTAALYAARSNLKVGLIEQGAPGGQMNNTAE
                     IENYPGYDHISGPELSMKMYEPLEKFEVEHIYGIVQRVENDGDVKRVITEDESYEAKT
                     VILATGAKNSLLGVPGEEEYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAVFLT
                     QFAKSVTIIHRRDQLRAQKVLQDRAFANEKIKFVWDSVVKEIKGNEIKVSGVTVENLK
                     TGEISEMTFGGVFIYVGLKPHSSMVSELGITDETGWVLTDTNMKTSIPGLYAIGDVRQ
                     KDLRQIATAVGEGAIAGQGVYNYITEHF"
     gene            31082..32542
                     /locus_tag="VBC94_000446"
     CDS             31082..32542
                     /locus_tag="VBC94_000446"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEO6978744.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFNKGIHNKKAVFEAYFRKVPFE
                     NGYAVFAGLERIVRYLENLSFSDSDLSYLEELGYPEEFLDYLKNLKMELTVKSAKEGD
                     LVFANEPLVQIEGPLAQCQLVETAILNIINYQTLVATKAARIRSVIEDEPLLEFGTRR
                     AQEMDAAIWGTRAAIIGGANATSNVRAGKIFNIPVSGTHAHALVQTYGDDYQAFKAYA
                     ETHKDCVFLVDTYDTLRVGVPNAIRVAKEMGEKINFLGVRLDSGDLAYLSKKVRQQLD
                     DAGFPNAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     TDAGSMRDTIKLSNNAEKVSTPGKKQVWRITSRAKGKSEGDYITFADTDVTQLDEIDM
                     FHPTYTYINKTVRDFDAVPLLVDIFDKGKLVYQLPSLQEIQEYGRKEFDQLWDEYKRV
                     LNPQDYPVDLARDVWQNKMDLIDRIRKEALAKGEVR"
     gene            32539..33360
                     /gene="nadE"
                     /locus_tag="VBC94_000447"
     CDS             32539..33360
                     /gene="nadE"
                     /locus_tag="VBC94_000447"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEO6978745.1"
                     /translation="MTLQDQIIKELGVKPVINPSQEIRRSVEFLKDYLLKHSFLKTYV
                     LGISGGQDSTLAGRLAQLAVEELRADTGENYQFIAIRLPYGIQADEEDAQKALDFIKP
                     DIALTINIKEAVDGQVRALNAAGVEITDFNKGNIKARQRMISQYAVAGQYAGAVIGTD
                     HAAENITGFFTKFGDGGADLLPLFRLNKSQGKQLLAELGADKDLYEKIPTADLEENKP
                     GIADEIALGVTYQEIDAYLEGKVVSDKSRGIIENWWYKGQHKRHLPITIFDDFWK"
     gene            33473..34807
                     /gene="pepC"
                     /locus_tag="VBC94_000448"
     CDS             33473..34807
                     /gene="pepC"
                     /locus_tag="VBC94_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEO6978746.1"
                     /translation="MSELTQTFTDKLLADYQANTKFSAIENAVTHNGLLKSLETRQSE
                     IENDYVFSIDLTKDEVSNQKQSGRCWMFAALNTFRHKLISDFKLENFELSQAHTFFWD
                     KYEKSNWFMEQIIATANQELSSRKVKFLLDVPQQDGGQWDMVVALFEKYGVVPKTVYP
                     ESVSSSASRELNQYLNKLLRQDAQILRELIAQGADGATVQNKKEELLQEIFNFLAMNL
                     GLPPQSFDFAYRDKDNHYQSDKNITPKAFYQKYVNLDISDYVSIINAPTVDKPYGQSY
                     TVEMLGNVVGGPAVKYLNLDMKRFKELAIAQMKSGETVWFGSDVGQVSNRQKGILATT
                     TYDFNSSMDIKLSQDKAGRLDYSESLMTHAMVLTGVDLDESGQPLKWKVENSWGEKVG
                     KDGYFVASDAWMDEYTYQIVVRKELLTKEELEAYNAEPITLAPWDPMGALAN"
     gene            complement(34854..37094)
                     /locus_tag="VBC94_000449"
     CDS             complement(34854..37094)
                     /locus_tag="VBC94_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6978747.1"
                     /translation="MITIKKESVIKLLKYAFGIIMGFIILAIVIGGLLFAYYVSRSPK
                     LTDQALKSVNSSLVYDGNNKLIADLGSEKRESVSADSIPLNLVNAITSIEDKRFFKHR
                     GVDIYRILGAAWHNLVSSNTQGGSTLDQQLIKLAYFSTNKSDQTLKRKSQEVWLALQM
                     ERKYTKEEILTFYINKVYMGNGNYGMRTTAKSYFGKDLKELSIAQLALLAGIPQAPTQ
                     YDPYKNPESAQTRRNTVLQQMYQDKNISKKEYDQAVATPVTDGLKELKQKSTYPKYMD
                     NYLKQVISEVKQKTGKDIFTAGLKVYTNINTDAQKQLYDIYNSDTYIAYPNNELQIAS
                     TIMDATNGKVIAQLGGRHQNENISFGTNQSVLTDRDWGSTMKPISAYAPAIDSGVYNS
                     TGQSLNDSVYYWPGTSTQLYDWDRQYMGWMSMQTAIQQSRNVPAVRALEAAGLDEAKS
                     FLEKLGIYYPEMNYSNAISSNNNSSDAKYGASSEKMAAAYSAFANGGTYYKPQYVNKI
                     EFSDGTNDTYAASGSRAMKETTAYMMTDMLKTVLTFGTGTKAAIPGVAQAGKTGTSNY
                     TEDELAKIEATTGIYNSAVGTMAPDENFVGYTSKYTMAIWTGYKNRLTPLYGSQLDIA
                     TEVYRAMMSYLTGGYSADWTMPEGLYRSGSYLYINGTTTTGTYSSSVYKNIYQNSGQS
                     SQSSSSTSSEKQKEDKNTADDANSSSPQVETPNNGNATTPNNSNQTVPGTGHGNGNNN
                     TAPNGN"
     gene            complement(37081..37680)
                     /gene="recU"
                     /locus_tag="VBC94_000450"
     CDS             complement(37081..37680)
                     /gene="recU"
                     /locus_tag="VBC94_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEO6978748.1"
                     /translation="MVNYPHQLIRKTTVTKSKKKKIDFANRGMSFEAAINATNDYYLS
                     HELAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYSGVYKGYYIDFEAKETR
                     QKTAMPMKNFHAHQIEHMANVLQQKGICFVLLHFSTLKETYLLPANELISFYQIDKGN
                     KSMPIDYIRKNGFFVKESAFPQVPYLDIIEEKLLGGDYN"
     gene            37755..38273
                     /locus_tag="VBC94_000451"
     CDS             37755..38273
                     /locus_tag="VBC94_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEO6978749.1"
                     /translation="MKSTILVTGYKNFELGIFQDKDPRITIIKKAIDKDFRRFLENGA
                     DWFIFMGNLGFEYWALEVALDLQKEYDFQIATIFTFENHGQNWNEANKAKLALFKQVD
                     FVKYTFPSYENPGQFKQYNHFLINNTQGAYLFYDSENETNLKFLLEMMEKKEAYDISF
                     LTFDRLNEIYEE"
     gene            38394..38726
                     /gene="gpsB"
                     /locus_tag="VBC94_000452"
     CDS             38394..38726
                     /gene="gpsB"
                     /locus_tag="VBC94_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEO6978750.1"
                     /translation="MASIIYSPKDIFEQDFKVSMRGYDKKEVDVFLDDVIKDYENYLE
                     QIEKLQMENRRLQQALDKKESEASNVRNSGTAMYNQKPIAQSATNFDILKRISRLEKE
                     VFGRQIRE"
     gene            38745..39113
                     /gene="rnpB"
                     /locus_tag="VBC94_000453"
     ncRNA           38745..39113
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VBC94_000453"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            39196..40350
                     /locus_tag="VBC94_000454"
     CDS             39196..40350
                     /locus_tag="VBC94_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEO6978751.1"
                     /translation="MKESFKLIATAAAGLEAIVGREIRNLGIDCQVENGRVRFHGDIK
                     TIIETNLWLRAADRIKIIVGEFPARTFEELFQGVYGLDWENYLPLGAKFPIAKAKCVK
                     SKLHNEPSVQAISKKAVAKKLQKVFHRPEGVPLQENGAEFKIEVSILKDKATVMIDTT
                     GSSLFKRGYRAEKGGAPIKENMAAAIIQLSNWFPDKPLIDPTCGSGTFCIEAAMIGMN
                     IAPGFNRDFAFEAWPWVDQSQVQKVRDEAESKANYDIDLDISGFDLDGRMVEIARKNA
                     EEAGLGDVIKLKQMRLQDLKTDKINGVIISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDEGFEKKYGSQADKKRKLYNGTLKVDLYQYYGERVRRQVK"
     gene            40363..>41788
                     /locus_tag="VBC94_000455"
     CDS             40363..>41788
                     /locus_tag="VBC94_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978752.1"
                     /translation="MSEDQKHPFFEPEKETDGLEFKDAKEMTVEEAVRKDSEIKAGIT
                     EEDSILDKYIKQHRDEVASQKFETKSSDFANLDTASLDDFIKKQREELSAMLAAEELS
                     KKLDNSVSQEQDIEANAVSPKEESSQEQENSVTPVPPLNTEAEPTATEPDSTIADSEE
                     YKSSSKKRGGIVGTLIALILLLIVTIFGYNYFKNNNSTNSQTATSQSSSSKATTTSSE
                     EDKKASQNLDNFNKAYANFFVDDKKTQLKNSEFDKLSELEKKVDALKGTKYYGKVKVK
                     FDSLKRQIDAVKAVNDKFKSPAVVDGKKSEKLEVKDGANFDSLDSKTLNTGNASLDSL
                     LHSIVSTGRNQVKQSEEQAKKTQEEQSSSNKVSDTQTTEQPNVTNGQSSSSAATINNQ
                     AAGTASGNLERNRSRVPYNNAAIADTGNPAWIFNPGVLEKIVATSQARGYFSGNNYIL
                     EPVNIINGNGYYNMFKLDGTYLFSI"
BASE COUNT        13537 a         6568 c         8501 g        13182 t
ORIGIN      
        1 tgtaatataa tacaaaacaa taggagttta caatgtcaat tattattggg ttaatggccg
       61 ctatgtttat cattcgttta gcttatttaa agctatctat agctaatgaa aaggccttaa
      121 gaaaaaatgg tgctaaagaa tatggtgtag gtgtttcaaa agcaatcaca gttcttcata
      181 ttatcattta tttttcatct gtaactgagg ctgttctaac aaaagcaagt tttaattttg
      241 ttagcgtaat cgggcttagc ttgatgattt tttctgtgtt tatgttacat acagtaacac
      301 gtttacttgg tcgtatttgg acagtgaaat taatggtaga taaaaatcat caatttgtag
      361 atcactggct atttagggtt gttaagcatc ctaactattt cttaaatatt gctcctgaac
      421 ttttaggtgt aactttattg tgtcatgcta agtatacagc attatttgtc ttgccaattt
      481 atgcttttgt tatttattta agaattcgtg aagaaaactt attattaaaa acgatcatta
      541 ttccaaatgg aatcaaaaag tctagggtat cttaagaata agataaatag tagacataaa
      601 agtcattttt tgttatactg tctcagtaag aagaggtgtt catcagctgt ttttttagcg
      661 agctcaaggt agtgaaagtt gggtagaata ggatgaatat tggcactttt tagtgagtaa
      721 aagtacaatc aaaaatgaag taataaatta gggtggaacc gcgtctttga cgcccctgtg
      781 ctgttgggca taggtggtgt aggacgtggt ttttatcatt tgtccgtttt aagtcaattg
      841 ttgtcacctt cccaacagct gtaataaagt atgaaagaag gaaaatgatg tctaagaaat
      901 taacttttca agaaattatt ctaactttac aacagttttg gaatgaccaa ggctgtatgt
      961 taatgcaagc ttatgataat gagaaaggag cagggacaat gagcccttat actttcttac
     1021 gtgctatagg ccctgagcct tggaatgctg catacgttga accatcacgt cgtccagctg
     1081 atggccgcta tggtgaaaat ccaaaccgtc tttaccaaca tcatcaattt caagtggtaa
     1141 tgaagccttc gccgtcaaat attcaagaac tttatttgaa gtctttggaa ttactaggta
     1201 tcaatccact tgagcatgat atccgttttg ttgaagataa ctgggaaaat ccatctacag
     1261 gttcagctgg attaggttgg gaagtttggc ttgatggaat ggaaataaca caatttactt
     1321 atttccaaca agtaggtgga cttcaaacag gtcctgtaac ttctgaagtt acttatggac
     1381 tagagcgttt agcctcttat attcaagagg tagattctgt ttatgatatt gagtgggcac
     1441 caggtgttaa atatggtgaa atctttactc agccagagta tgagcattca aaatattcat
     1501 ttgagatatc agaccaagta atgttacttg aaaactttga gaaatttgaa agagaagcaa
     1561 aacgtgcttt agaagaagga cttgttcatc cagcttatga ctacgtcctt aaatgttcac
     1621 acacctttaa tttattagat gcacgtggag cagtatctgt tactgagcgt gcaggctata
     1681 ttgcacgtat tcggaattta gcacgtgttg ttgctaaaac ctttgttgcc gaacgtaaaa
     1741 aacttggttt cccacttttg gatgaggaaa ctcgtatcaa attacttgct gaggaagact
     1801 aatgaacaca ctcattgtta atagtcatcc tgatttttcg aatccgtact catttacaac
     1861 tattctacaa gaaaagttta ttgaacttta taatgagcat ttccctaacc atcagttatc
     1921 tattttaaat ttatatgatt gtgttttgcc agaaattacg aaagaagtac tactttcgat
     1981 ttggtctaaa caaagaaagg gtttggaatt aacagccgat gagattgttc aagctaaaat
     2041 ctctaaagat ttacttgaac agtttaagtc acatcaccgt attgtctttg tttctccaat
     2101 gcacaattac aatgtgactg ctagagcaaa aacttatatt gataatattt ttattgcagg
     2161 tgagactttc aagtacactg aaaatggttc ggtcggatta atgacagatg actatcgtct
     2221 gcttatgtta gaatctgcgg gttctattta ttctaaagga caatatagtc catatgaatt
     2281 tcctgttcat tatttaaaag ctatctttaa agattttatg gcttttgatg atttctgtgt
     2341 tgttcgtgca gaaggtaccg atattttaga taggcaagtt gttcttgata agatgaatca
     2401 ggatttaaga gaagcatttg aagcgtttta tagtaaggag taggaaatga ctaaagattt
     2461 attattggag cttggacttg aagagttgcc agcttatgtt gtgacaccga gtgagaagca
     2521 attgggccaa aaaatggtca agtttttaga ggatcatcgt ttatcatttg aaactgttca
     2581 aactttttca actccacgtc gtttggctgt tcgtgttaaa ggattggcag accagcagac
     2641 agatttaaca gaagatttca agggtccttc taagaaaatt gctttggatg cagaagggaa
     2701 tttttctaaa gcagcccaag gttttgttcg cggaaaaggt ttaagtgttg atgatatcga
     2761 atttcgtgaa gttaagggag aagaatatgt ttatgtcact aagcatgaaa ctggaaaaag
     2821 tgcgattgat gttttagcga gtgtgacaga ggtattaaca gaattgactt tcccagtgaa
     2881 catgcattgg gcaaataata gttttgaata tattcgtcca gttcacactt tagtggtatt
     2941 attagatgat caagccttag agctagactt ccttgatatt cattcagggc gtattagtcg
     3001 aggacaccgt ttcttgggtt ctgacactga aatttcatct gccagttcgt atgaagatga
     3061 cttgcgacaa caatttgtta ttgctgatgc aaaagaacgt cagcaaatga ttgttaatca
     3121 aattcatgct atcgaagaaa agaaaaatat ttcagttgaa attgacgaag atttactaaa
     3181 tgaagtgctt aatttagttg agtatccgac agcattcttg ggttcatttg acgagaaata
     3241 ccttgatgta cctgaagaag ttttagtcac ttcaatgaaa aatcatcaac gctattttgt
     3301 tgttcgcgac agggatggaa aattgttacc aaactttatt tctgtgcgta atggaaatgc
     3361 tgagcatatc gaaaatgtta taaaaggcaa tgaaaaagtt ttagtggctc gtcttgaaga
     3421 tggtgaattc ttctggcaag aagatcagaa acttaatatt gcagatttag ttgagaaatt
     3481 aaaacaagta actttccatg aaaaaattgg ttcactttat gaacatatgg atcgtgtaaa
     3541 agttatttca caacatttgg ctgataaggc aggtctttct gatgaagaaa aattagctgt
     3601 gcttcgagct gcaagtattt ataaatttga cctcttaact ggaatggttg atgagtttga
     3661 tgaattacaa ggtattatgg gtgagaaata tgccttactt gcaggggaac agcccgcagt
     3721 agcagcagcg atacgtgaac attatatgcc gacatcggcc gatggagagc ttccagaaac
     3781 cagaattggt gcaattctag cgttagctga taaatttgac accttacttt cattcttctc
     3841 agttggtttg attccaagtg gttcaaatga tccatatgct cttcgtcgag ctactcaggg
     3901 gattgtccgt attttggaag cttttggttg ggatatccca cttgatgaat tagtaactaa
     3961 cctttatggt ctatcgtttg ctagtttaga ttatgctaat caaaaagagg tcatggcctt
     4021 tatttcagct cgtattgaaa agatgattgg ctctaaggtc cctaaagata ttcgagaagc
     4081 cgttttagaa tcagacactt atattgttag ccttattcta gaagctagcc aagcattggt
     4141 gcaaaagagc aaggatgctc aatataaagt ttccgttgaa agtttgtcac gtgcatttaa
     4201 tttagctgaa aaagttactc attctgtatc ggtggactct tcattgtttg aaaataatca
     4261 ggagaaggcg ctatatcaag ctattttgtc acttgaactg actgaggata tgcatgacaa
     4321 cttagataag cttttcgcac ttagtccaat tattaatgat ttctttgata atacaatggt
     4381 aatgacagat gatgaaaaaa tgaaacaaaa tcgtctggct ttactcaata gccttgttgc
     4441 aaaggcaaga acagtcgctg cttttaactt attaaacaca aaataaggag gcattttatg
     4501 gatcctaaaa aaattgctcg tattaacgag ctttcaaaaa agaaaaaaac agttggattg
     4561 accggagaag agaaggttga acaagcaaag ctccgtgaag agtatattga aggtttccgc
     4621 cgatcagtcc gtcaccacgt tgaaggaatt aaattagtgg atgatgaggg caatgatgtt
     4681 accccggaaa aacttcgtca agtccagaga gaaaaaggac tacatggtcg tagtttggat
     4741 gatccaaatt cttaatggtg agacaatttt aaattaaagt caacacttgg gtggggcaaa
     4801 agataatctt tgtctcgcct tttttattag gaaggaaaaa ataatgattg aattactatt
     4861 agcattactg ttatggaata ttttagtatt tgcaacctat gctattgata aaaggcgtgc
     4921 aattagaggg gcatggcgta ttagtgagaa aacattgttg atagagagtt tattattagg
     4981 gggatttggt gcattcctag gtggagaagt ttttcgacat aaaacgacta aatggtattt
     5041 tagactagtt tggtatatcg gaatgattat tgatttagct attatttggc taatttggtg
     5101 ttattgatga tatcttatca aaaattttag gggcatatta aatttttgat agtgcaggat
     5161 agactgattt aggtataata gaattacaaa aagaaaacgc ttacttttaa ggaggaagtt
     5221 tatgtcttca gaagaaaaat acgttatggc gattgaccag ggaacaacaa gttctcgagc
     5281 aattattttt aataaaaaag gtgaaaaaat tgcgagtagc caaaaagaat ttccccaaat
     5341 ttttcctcaa gcaggctggg tagagcataa tgctaatcag atttggaatt ctgttcaatc
     5401 tgtcattgct ggagctttta ttgaatcaag catcaaacct ggacagatag aggctattgg
     5461 tattaccaat caacgtgaaa caactgttgt ttgggataaa aaaacaggtc ttccaattta
     5521 taatgctatt gtatggcaat cgcgacaaac agccccgatt gctgatcaat tgaagcaaga
     5581 aggtcatact aatatgattc atgaaaaaac tggtcttgtc attgatgctt acttctcggc
     5641 aacaaaagtt cgttggattt tagaccatgt tcctggagca caggaacgtg ctgaaaaagg
     5701 agagttgctt tttggaacaa ttgatacttg gttagtttgg aagttaactg acggtctcgt
     5761 ccatgttaca gactattcaa atgctgctcg tacgatgctt tataatatta aagaacttaa
     5821 atgggatgat gaaattttag agttactgaa tattcctaaa gcaatgttac cagaagtaaa
     5881 atctaattct gaagtttatg ggaaaactac tccattccat ttttatggtg gagaggtgcc
     5941 aatttctggt atggctggtg accaacaggc agcattattt ggacagttgg cctttgaacc
     6001 tggtatggta aaaaacactt atggtacagg ttccttcatt atcatgaata cgggcgaaga
     6061 gatgcaactg tctcaaaata atctattaac aacgattggt tacggcatta atggtaaggt
     6121 tcattatgca cttgaaggat caatcttcat tgcaggaagc gctattcaat ggttacgtga
     6181 tggtcttaga atgattgaaa cgtcaagtga gtcagaaggc cttgctcaat catcaacaag
     6241 tgacgatgag gtttatgtgg ttccagcttt tacaggtttg ggtgcaccgt attgggattc
     6301 aaatgctaga gggtcagtat ttggattaac tcgtgggaca agtaaagaag attttgttaa
     6361 agctacactt cagtcaattg cttatcaagt acgagacgtt attgacacta tgcaagttga
     6421 ttcaggaatt gatatccaac aacttcgtgt agatggtgga gctgcaatga ataatttatt
     6481 aatgcaattc caagcggata ttttagggat tgatattgct cgtgcgaaga accttgaaac
     6541 tactgccttg ggtgcagctt tcttagccgg actttcagtt ggctattggg aaagtatgga
     6601 tgaactaaaa gaactgaacg caacaggaca acttttccaa gcaacaatga atgagtctcg
     6661 aaaagaaaaa ctgtataaag ggtggagaaa agctgtcaaa gcgacacaag tatttgctca
     6721 agaggattaa gggggtgtag agatggaatt ttcacgagaa acacgacgtt tagccttaca
     6781 aagaatgcaa gatcgtactc tggatttact tatcataggt gggggtatca caggcgctgg
     6841 tgtcgcttta caagcagcag ctagtggatt agatactgga cttattgaaa tgcaagattt
     6901 tgcagaaggg acaagtagtc gctcaacaaa attggtccat ggtggtcttc gttacttgaa
     6961 gcaatttgat gtagaagttg tttctgatac agtatcagag cgtgcagtcg tgcagcaaat
     7021 tgcaccacac attcctaagc cagatccaat gcttttaccg gtttacgatg agccgggttc
     7081 tacttttagt atgtttcgcc ttaaagtggc tatggattta tatgatttat tagcaggtgt
     7141 gacaaacaca cccgcagcca ataaagtttt aagcgcagaa gatgttctta agcgtgagcc
     7201 tgatttacaa aaagaaggtt tgctaggcgg aggggtctat ttagatttcc gtaataacga
     7261 tgcacgccta gtgattgaaa atattaaacg tgctaatagg gatggagctt atattgccag
     7321 tcatgtcaaa gctgaggact tcttatttga tgacaacaat caaattatag gagtacgtgc
     7381 gcgtgatttg ttgactgacc aagttatcga tataaaagct agacttgtta ttaatacaac
     7441 tggaccttgg agtgacaccg ttcgcaactt ctccaatgaa ggtaaacaaa ttcatcaatt
     7501 acgtccaact aagggagttc acttagttgt agatcgccaa aaattaaatg tttctcaacc
     7561 tgtttatgtt gatacaggtc taaatgatgg tcgcatgatt tttgttttgc cacgtgaaga
     7621 taagacttat tttggaacca cagatactga ttaccatgga gacttagaac atcctacggt
     7681 aactaaagaa gatgttgact acctccttaa tattgtaaac aaacgcttcc cagaagcaga
     7741 gcttactatt gatgatattg agagttcatg ggcaggtctg cgtccactac tatcagggaa
     7801 tagtgcctct gactataatg gaggcaatag tggaaaacta agcgatgaaa gttttgaaga
     7861 attaattgat tccgttaaag attatattgc acataaaaat catcgtgagg atgttgaaaa
     7921 agctatttct catgtagaat ctagtacttc tgaaaaagaa ttggacccat cagctgtttc
     7981 tcgaggatca agttttgagc gtgatgataa tggcctatta acacttgctg gtggtaaaat
     8041 tactgattat cgaaaaatgg cagaaggtgc catggaaact attattaaca ttttggataa
     8101 agaatataat cgcaagttta aactaatcaa ttctaaaacc tatcctgttt caggaggaga
     8161 gattaaccca tctaatgttg attctgaaat tgaagcttat gctcaattgg gcacactaag
     8221 tggtttaagt attgaggatg cacgttatat tgctaacctt tatgggtcaa atgcaccaaa
     8281 attatttgca ttgacacgcc aaattacaga agcagaaggt ctgagcttgg tagaaacgtt
     8341 gtcacttcac tatgctatgg attatgaaat ggcgcttagc ccaacggatt tcttcttaag
     8401 acgtacgaat catatgcttt tcatgagaga taatttagat tctttgattc agcctgttat
     8461 tgatgagatg gctaaacatt atcaatggtc tgatcaagac aaaactttct atgaagaaga
     8521 attacatgag actctaaaag acaatgactt ggcagctttg aaggataatt gaaggagata
     8581 ataatggata tttttggaga attcttagga acagctctct tagtcttact tggtaatggt
     8641 gttgtagcag gtgttgtttt gcctaaaacg aagaatcata attctggatg gattgttatc
     8701 acatttggat gggggcttgc tgtagctatt gcagcgttgg tatctggcaa tattagccct
     8761 gcccacttga atccagctgt gtcattagct tttgcgatca aaggtgattt agcttgggga
     8821 acagctattc tttatatgat tgcacagatt attggtgcaa tgctaggttc acttcttgtt
     8881 tatttgcaat ttagacccca ttatgaggct gcagaaaata aagcagatat ccttggaact
     8941 tttgcaactg gtccggcttt gaaggataat ttttccaatt ttttaagtga agtattggga
     9001 acacttgttc ttgtacttac tatttttgcc atcggaaaat acaatatgcc tccaggagtt
     9061 ggtacgatga gtgttggtat gttagtggtt gggattggtc tatcgctcgg tggaacaaca
     9121 ggttatgcta ttaatcctgc acgtgatttt ggtcctcgtt tattgcatgc cttgttaccg
     9181 atgaagaaca aaggtgattc tgattggact tattcatgga ttccgatagt aggacctatg
     9241 gtaggagcta ttttagcagc cttaatattt gctatgatgt aagagatttt tgatgctgag
     9301 accgacttta tggtctcagt tttttgttac cgttttcaaa aataacttct tttgttataa
     9361 taagtaatta tgacagaaaa atatgtaaat aaagtaattg tcattttagg agcttctttt
     9421 gctggtatga cttgtgcgca gaaattacgc caattaaatc ctaattggga tatcgttttg
     9481 attgataaag aaattcatcc agattatgtt ccaaatggct taaattggta ttatcgtcac
     9541 gaaattagtg gtttaaatca ggccatgtgg cagacagaag aagagcaacg gttgcagaat
     9601 atacgctgtc tatttggtct aaaagttgag aaaattaata aagaagatag ggaattaatg
     9661 ttatcagatg gttcttctgt ctattatgat caattgattt gtgccatggg ctcccaagca
     9721 gaatcaactt atattgatgg ggctgatgcg caaggtgttt taacaacaaa aacttatgca
     9781 actagtcaaa atgctaagca ggtattagat aaaagtcata aggtagcagt tgttggggca
     9841 ggtatcatcg gtttagatat tgcttatagt ttacatgaat cagggaaggc ggtcactttg
     9901 ttagaagctc aagaaagacc agacttcagg catacagatc ctgatatgtc ccttccttta
     9961 cttgatgcga tggctgaatc aaaactacat tttttccaaa atcaaaaggt tgagaaaatt
    10021 actgtaaccc gagaagaaaa actttgtttg cgaacgctaa caggagacac ctttacagtt
    10081 gatactgtca tattagctgt taattttcgt ccggatagtc gtcttttaac tgggttggtt
    10141 gatttaagtg ttgataatag tgttgttgta aatgattatt tccaaacgag tgaccctaat
    10201 atctatgcta ttggcgattt gatttggtcg tattttaaag gtcttaatag tgcttattat
    10261 atgcccttaa ttaaccaagc aatccgttcg gcacaaatgt tggcatacca tttatcggga
    10321 catgctgttc caaaattaaa aataacacgc gctacaggat ctaaacactt tggctattat
    10381 agagctaaca ttggattaac tgagctggaa gctggtcttt acgaggatac cgttagtgtt
    10441 acttatttcc ctaaagaaca gtatgattta aggataaaat tgattgctaa tcaaaaaacg
    10501 ggccatttat tgggggctca attaatctca aaggaaaatt gtttggctac tgccaatcag
    10561 cttgttcaag ctatatcgtg tgacatgaca gattttgatt tggcttttca agatttcatt
    10621 tatacagcta gagaatctga gatggcttat atgttacatc aagcagctat caacctttat
    10681 gaaaagagga ttgggctatg cgaatagaag ctctaatgga aaaagaaagg cgtgttcaat
    10741 atcggctact ttctttttta agaggctctc ctcaggcaat agcactgaag ttagctttat
    10801 tagaaactgg tttaagtcga gcaactttgc ttaaatatat caataatctt aatagctact
    10861 ttgaacaaga aaaagttaat tgtcgaatcg tttattataa ggataagtta tttttagaag
    10921 aggattataa cttatccaat caagaagtgt tgaaagctct gatgaaagat agtattaaat
    10981 acactatctt aatatcttta tttaatcagc gtcagttcac aatcgttggt ctttctcaag
    11041 aattgatggt tagtgaggct actttaaatc gtcatcttgc tcatctcaat gaattgttag
    11101 cggaatttga tattgctatt tcacaaggaa aacagattgg tgatgaattg caatggcgtt
    11161 atttttatta tgaattattc aagcaactat ggtcttatga caagtgccaa aatatgataa
    11221 agaaattaga cttagactca ttaattctat taatagagcg cttggctcaa catactttaa
    11281 ctcgagaagc tcagcaaaat ctggggcttt ggtttagtat ttgtcatcat cgtctccttg
    11341 ctatggaaaa actatctgat aatctcaagc ccattgtgga acattatcaa tgtaatgctt
    11401 tttataaacg gttagacgca gccttagttt tgtatatgag tcgttttgct cttgagtata
    11461 gggaaggaga agttctggca acttttgctt ttctacattc acaaaatatt ttaccaatca
    11521 atacaatgga atacatcatg ggatttggag gcccgattat tgattgtgta accgaaacaa
    11581 tcatctactt taaaaaggag agtattttag ctgacgagac atcagatcag gttatttacc
    11641 aacttggtca gttatatagc cattattatt tctttaaagg tcatatttta gttgaacaac
    11701 ctgatttaga acaaacgtat cgacttattg atcataatat gagggataag ttgcatgata
    11761 tttcaaagaa gattattgca aatgttaatc gaattagacc tttaacagaa gatggttgtt
    11821 ctttgctgac tttacatttg ttggaattgc ttattttctc gaaaaatagt caaaaaatgc
    11881 cctttaggat tggcctagat atgacaggta atgctgtgga acagtcattg ttggaatatc
    11941 gcattcgaca acatttctct ggtaataatt ctattcaagt cgaaccttat gacgaaggaa
    12001 agggatttga tatggttatt tatcagagtc gctcaagacc ttataaagcc aaactaacct
    12061 attgccttaa taaaggtgct tcagagcgag aactacagga aatagattca cttatttatg
    12121 acaattaatc ccattactta attgaatggt tttaattcct ccagaaattg ttaaaacgtt
    12181 tacgtatttt atgttataat atcaaagacg aacaatttta aatttagttg ttaaaaacag
    12241 aaaggtttta tcatgacatt tgacaccatt gatcaattag cggttaatac agtccgcacg
    12301 ctttctattg atgctatcca agcagcaaat tctgggcacc caggtcttcc tatgggagct
    12361 gcgcctatgg cttatgtgct ttggaataaa ttcttaaatg taaacccaaa aacaagtcgc
    12421 aattggacaa accgtgaccg ttttgtactt tcagctgggc atggttcagc tcttctttat
    12481 agcctacttc atttagctgg ctatgattta tcaattgatg atttaaaaca attccgccaa
    12541 tggggatcaa aaacacctgg acatccagaa gttaaccata ctgatggtgt agaagcaaca
    12601 actggacctt tagggcaagg tattgctaat gctgttggta tggcaatggc tgaagctcat
    12661 ttggctgcta aatttaataa accaggcttt gatttagttg atcattacac ttatacactt
    12721 catggtgatg gttgtttgat ggagggtgtt agccaagaag ctgccagtct tgctggacac
    12781 ttaaaacttg gtaaattggt ccttctttat gattcaaatg atatctcact tgatggtcca
    12841 acgtctcaat catttacaga agatgttaaa gggcgttttg aatcatatgg ttggcaacat
    12901 atcttagtta aagatggtaa tgatttagaa gcgattgctg cagcaattga agctgctaaa
    12961 gctgaaactg ataaaccaac cattattgaa gttaaaacta ttattggatt tggcgcagag
    13021 aaacagggaa catcttcagt tcatggagcg ccacttggtg cagaaggtat tacttttgcc
    13081 aagaaggctt atggatggga atatccagac tttactgttc ctgctgaagt tgcagctcga
    13141 tttgcctcag atcttcaagc gcgtggtgcg aaggcagaag aggcatggaa tgatcttttt
    13201 gctaaatatg aagttgagta tccagaatta gcagctgaat acaaagaagc ttttgctggc
    13261 caagctgaga cagttgaatt gaaagctcat gacctaggtt catcagtggc tagtcgtgtt
    13321 tcaagccaac aagctattca acaattatcg actcaattac cgaacctctg gggagggtca
    13381 gcagacctct cagcttctaa taacacaatg gtagcagcag aaacagattt ccaagcaagt
    13441 aactacgctg ggcgtaacat ttggtttggt gtacgtgaat ttgcaatggc tgctgcaatg
    13501 aatggaattg cgcttcatgg tgggactcgt gtttatgggg gtacattctt tgtcttctca
    13561 aactacttgt taccagcagt acgtatggct gcacttcaaa acttgccaac tgtctatgtt
    13621 atgacacatg attctattgc tgttggagaa gatgggccaa ctcatgagcc aatagaacaa
    13681 ttagcatcag tgcgctcaat gcctaatttg aacgttatcc gtcccgcaga cggtaatgaa
    13741 acaaacgctg catggcaacg agctgtatca gagacagatc gaccaacgat gcttgtttta
    13801 acacgtcaaa acttaccagt tcttgaagga acatcagaat tagcccaaga aggggttaat
    13861 aaaggtgctt atattttatc agaagctaaa ggtgaacttg atggtatcat cattgcaaca
    13921 ggttcagaag ttaaattagc tttagatact caagacaaac ttgagtcaga gggcatccat
    13981 gttcgcgtcg tatctatgcc agcacaaaac atttttgatg aacaagaggc ttcatatcaa
    14041 gagcaagtgc ttccatcagc tgttacaaaa cgtcttgcta ttgaggcagg atcaagcttc
    14101 ggttggggta aatatgttgg tttaaatggg ctaacactta ctattgatac ttggggagca
    14161 tcagcaccag gtaacagaat tttcgaagag tatggcttta cagttgagaa tgctgtatcg
    14221 ctatataaag aactttaaga gcatgaagaa gttgggaaaa atacctcaac ttctttttgt
    14281 aagatatagg atggatttga gaatagtttt gatagactga taagtgatgg aaagcactct
    14341 tttttgaaaa cgtttgacaa acattccata gcgcagtata attgataaaa ctattgtata
    14401 tataaaataa tacaactatt attaattatc tttttagaaa ggtttgttta tgaagaagga
    14461 gtgtcgtgat ttttatcggc agatacagca tacttacaat gatatatctg tccgtgaaga
    14521 tgcagtgcta tcatcaatac tattatcagc atcgaatggt ttgataaaaa ctagcgatgt
    14581 acctcgtgtt gcttatgagt taactcagca attggaaaat aatgagatag aaaaatcttt
    14641 cgagtcatta gctacagtta aggaattaaa aaaatctgct aaaaaattgt ctgagagata
    14701 tgagcaaaat aaggtaacaa cgggattgct ttctaaagta tttggaaatc attaggagat
    14761 aaaaggagat ttttatgatt aagtttgaac atgtttcgaa agtatacggg gaaaaagaag
    14821 ctctcagtga tttaaccctc tctattaaag atggtgagat ttttggatta atcggtcaca
    14881 atggagcagg aaaaacaacg acaattagta ttctaacatc tattattgat gcaacttatg
    14941 gccaagtcta tattgatgac ctacttttga cagaacatcg tgaccaaatt aagaaaaaaa
    15001 taggttatgt accggactcg ccagatattt tcttgaattt aacagcagag gaatactggt
    15061 actttttagc taaaatttat gatgttgccc cagaagatat tgaagcacgt atcacaaaat
    15121 tggtagacat atttgaacta gaggagcagc gctataatcc tattgaaagt ttttcacatg
    15181 gtatgcgtca aaaagtcatt gttataggtg cccttcttcc aaatcctgac atttggattc
    15241 tagatgagcc attaactggt ttagatcctc aagcttcttt tgatttgaaa gaaatgatga
    15301 aagaacatgc taaaaatgga aaaacagtta ttttttcaac acatgtctta gcagttgcag
    15361 aacagttatg cgacagaata ggtattttga aacaagggaa attaatcttt gtcggtagct
    15421 taggtgagtt aaaaatgcaa tatccagata aagatttgga aactatctat ttggaattag
    15481 caggacgtca agctaatagg gagggataaa gtatgaattg gtcacgtatc tgggaactcg
    15541 taaaaattaa tatcctttat tcaaaccctc agactctatc ggcactaaga aaaaagcaag
    15601 aaaagcatcc taaaaaagaa ttttcagctt ataaatccat gtttagaaat cagttatttc
    15661 agattttgct cttttcaata atttatgtat ttctctttgt atcacttgat tttaaagaat
    15721 atccgggcta tttcacgttc tacattggta tctttacact agtatccatt atctactctt
    15781 ttattgcgat gtacagtgtt ttctatgaga gtgacgatgt taaacaatat gcttatttgc
    15841 ctattaaatc tgaagagctt tatgttgcta aaatttttgc aacatttggg atgtctgtaa
    15901 cttttctaat gccaatccta accttaatga ttgtggctta ttggcgtata ataggtggcc
    15961 cacttgcggt cctcctagca atcataaatt ttgctatttt atttttatcg gtaacggtga
    16021 tttcattgta tattaatagt ttaataggcc gtgccattat tcgaagtgct aatcggaaac
    16081 ttatttctac tatattaatt tcactagcta catttggagc gatagttccc ttattatttg
    16141 tgaatatgac cagtcaaaag atggtacaag ggaaattaca agatatcgct ccaattcctt
    16201 atgtcagagg ttattatgat attgttacag cgccgtttag tatggagagt cttctcaact
    16261 attatctccc gctcttaatc attttattct tgataggtgc tatttataaa tgggttatgc
    16321 cacgctacta tcaggagttg ttgtatggac aggtcaagca gagaaaagtc catcgtcaaa
    16381 tcgattttag taaacgtgag tcaattaata aaactcttgt taaacaccat ttatcaagtt
    16441 tacaaaatgc gacattattg acaaatacat ttttaatgcc actattatat ctagcaatgt
    16501 tcattgttcc aattttgaat aatggtaaag agattggtcg attttttaat gaaaattatt
    16561 ttgggatagc ttttctagca ggaatattaa taggtagttt atgtgtgatg ccagctagca
    16621 ttgtaggggt aggtatttct ttagaaaaaa gtaatttcta ctttataaaa agtttgccta
    16681 tatcattttc gtatttctta aagcataaat ttgtaacttt gataacatta caacttgcag
    16741 ttcctacttt tatttatttc ttggtaggat tctttctact taaactatca atcctcgttt
    16801 tattgagctt cattttaggt ctagtcttca tgggacttat cgaaggccag tttatttata
    16861 gacgtgacta taaacatttg ttcttgaatt ggcaagaggt tacgcaacta tttaatagag
    16921 ggttaggcca atggctgtta gttggttcat tgtttggaat gatgataata ggtagttttc
    16981 ttattggaat aagtattttt tggtcaatgg tatggaatac tgtagctgtc aatatcataa
    17041 tccttatcat tgggttgctc atattatcaa tttgccaata cttactgcta aaaaattttt
    17101 ggaagaagct tgtttagtaa aaagatttta gaaaaatcat acagtaagaa taatgcttaa
    17161 taactgagat agttatagcg gataaaataa aggcttgttg tggactttac ggttggaagg
    17221 cagccactgt aatcctttgt ttaataacgc tagttttagc tctaaaatat ttgattttta
    17281 ttgctatcta ttatatctta tcagacaaag agactaggga taacctagtc tctttgtctg
    17341 atgctaactt gtttttcgta atattgtgct agaatgaatg tcaatatagc agttgtgctt
    17401 acagtaatga taactgcaat aagtatggta ataaaaagat gtgttgaagt atgactgata
    17461 taaagaaata gacctggtag accagaagag cctacagcga agcggtgagc gttcattata
    17521 ccggcataga gtcctccaag accaccacca ataagtgagg ctattagagg ataacgtttc
    17581 ggaaggttga caccatataa gataggttcg gtgattccca tataagctgt tatggcagca
    17641 ggtccagcaa tttctttaag ctttttttct tttgttataa gagtcaccac tgtacctgca
    17701 gttgcttgtg cgatatttga gcagagcatg cctggtccaa aaatagaatc aacccctaat
    17761 ttcgctagtt gtgtgattcc taatgcagca attccactat gaacaccaaa catgattagg
    17821 ataggagcaa atgctcctac tagaaaagca ggtgcccaac tggcgtattt ggataaaaaa
    17881 agtaaaaata acagctaaat attggccagc gatagtaccc attggtccca atatagagaa
    17941 cgacaaaatt cccatgctga gaaatattaa cataggaaca agcactaaac gtagtgattt
    18001 aggaataatt tgatttaaat atttttctat ataagcctga acacaaataa ttagaataat
    18061 gggaatgaca cttgatgcat agttagtaag tgtgaatggg atcgtatgga ataaggatac
    18121 tggtttacca gaagctacta aattagccca attagggtga aggagcatga caacagttcc
    18181 caaagctaaa ataggattag ccttcaattt ttgagcagct gtgatggcga ttaagatagg
    18241 taaaaaatag aaaacaccat ctgcaaaaag atttagtaat atatatgttt gagataacgg
    18301 tgtcaagatt ttgaatacta gtaataaagc tagcaatgcc ttaatcatac cagcgcctga
    18361 tagagctggt aaaatcggtt ggaaagtccc agaaacaaaa tcaataattc gactaactgc
    18421 tttggctttt ttggtttgtt ctgttttagt cgttgagtta gtttcaatga gggagttaat
    18481 ttcttcaaac acttttgcga catgagtccc gatcactatc tgatgttgcc ctccgctgat
    18541 aacgacagtt tttacctctt ttaaagtttt taattgctca aggtttactt tcgtcggatc
    18601 attgagacta aagcgaaggc gtgtttgaca atggtaaaca ttgttaatat tattttgccc
    18661 accaaggtgt ttgatgatat tttttgacaa ggtgatgtat tctctagaca taatatctcc
    18721 ttaatactta taaaacgttt tccgattcta gattatagca tagtaataaa agaaaacact
    18781 tggaaatgct tgtgaatttt caatgatgtt ttcagatagc attcctaaag ggtctatgat
    18841 ataatttttt tatgaaaaga cattttgaga caactagaag aattgttatt aaagtaggaa
    18901 ccagttccct cgtacagaca agtgggaaaa ttaatttaag taaaatagac catttagcat
    18961 ttgttatttc tagtcttatg aataaaggta tggaggtgat tttagtatca tctggagcta
    19021 tggggtttgg tttagacatt ttgaaaatgg ataagcgtcc tcaagaaata tcacaacaac
    19081 aagcagtttc tagcgtagga caagtagcca tgatgagtct ttattctcag atattttcgc
    19141 actatcaaac tcatgtttcc caaattttat taacacgtga cgttgtggtt tttccagaga
    19201 gtttgcaaaa tgtaacaaac tcttttgaga gtctgctctc tatgggaatt ttgccaatcg
    19261 tcaatgagaa tgatgctgtc agcgtggatg agatggatca taaaacgaag tttggggaca
    19321 atgaccgtct ctctgctgtc gttgctaaaa ttactaaagc agatttatta attatgctgt
    19381 cagatattga tggtcttttt gataaaaatc ctaatattta tgatgatgct gttttacgta
    19441 gtcatgtttc tgagatcacc gatgacatca ttaagtcagc tggaggagca ggaagtaagt
    19501 ttggtactgg tggcatgctt agtaaaatta aaagtgcaca aatggtgttt gataacaatg
    19561 gacaaatgat attgatgaat ggcgccaatc ctcgtgatat tttaaaggtg ttagatgggc
    19621 ataatattgg aacttatttt gctcaaggaa agtgagattg agttatgacg tatattgaaa
    19681 tattagggca aaatgctaaa aaagctagtc aatcagtagc acgattaagt acagctagta
    19741 aaaatgagat actcagggac ttagcgagaa acatagtggc tgatactgaa accattttga
    19801 ctgagaatgc tcgcgatgtt gctaaggcaa aagataatgg tatctcagaa attatggtgg
    19861 accgtctacg actaaacaaa gaccgcatac aggcgatagc aaacggtatt tatcaggtag
    19921 cagatttggc agatccgatt ggtcaagttg ttagtggtta tacaaattta gatggtttaa
    19981 aaatccttaa aaaaagagtg cctttagggg ttattgcaat gatatttgaa agtcgtccca
    20041 atgtatcggt ggatgccttt agtttagctt ttaaaacagg gaacgctatt attttacgtg
    20101 gtggtaaaga tgctattttt tcaaatactg ccttggttaa ttgtatgcgc caaacattgc
    20161 aagatacagg ccacaaccca gatatagtac aattagttga agatactagt catgttgtag
    20221 cagaggagtt aatgcaggca acagactacg tggacgtctt aattccaaga ggtggtgcaa
    20281 aattaattca aacggtgaag gaaaagtcaa agattcctgt tatcgaaaca ggtgttggaa
    20341 atgttcatat ctatattgat gaatttgcag atttagatat ggcagctaag attgttatta
    20401 atgctaaaac ccaacgccct agtgtctgta atgctgctga agggttagtt gttcatcaag
    20461 ctattgctaa gggattttta tctcaattgg agaaaatgct caaagaatcg aaccagtcag
    20521 ttgaatttcg tgccgatgag gaagctttac aacttttaga aaatgctgtt gctgcaagtg
    20581 agactgatta tgctactgaa tttttagact atattatgtc agtaaaagta gtagattctt
    20641 ttgaacaagc aattagctgg attaataaat acagtagtca tcactcagaa gctatcatta
    20701 ctaataatat cagtagggca gaaatttttc aggatatggt agatgcagcg gcagtttatg
    20761 ttaatgcttc aacacgtttt acagatggtt tcgtttttgg actcggagca gagattggta
    20821 tttcgactca aaaattacac gcgcgtggac cgatgggatt agaagcttta acaagtacaa
    20881 aatactatat taatggaact ggtcaagtaa gggaatagtt gacctataat aggagagatt
    20941 gcatttatcg gtctctcttt tacttttcta aagtttagga tttatggtac aattaaagtt
    21001 atgacaaacg attttcatca tattactgtg cttttgcacg agactgtgga tatgcttgat
    21061 attaagccgg atggtaccta tgtagatgct actttaggtg gggcaggaca cagcgaatat
    21121 cttctttcac aattaggccc agatgggcac ctttacgcat ttgaccaaga ccagaaggct
    21181 attgacattg ctcatatacg tttaaagaaa tatgtggata ccggtcaagt aacctttatc
    21241 aaagataatt tcagaaatct atcttctaac ttaaaagcat taggagtgtc ggaaattaat
    21301 ggtatttgtt atgatttagg tgtatcaagc ccacagctgg acgagcgtga acgcggtttc
    21361 tcatataaac aagatgcccc ccttgatatg cgaatgaata gagagcagag ccttacagct
    21421 tatgacgttg tgaacactta tagttatcat gatttagtaa ggattttctt taagtatggc
    21481 gaagataaat tttcaaaaca aattgctcga aaaattgagc aagtacgagc tgaaaaacca
    21541 atatctacaa caacagaatt ggctgagatt atcaaatcgt ctaaatctgc caaagaactg
    21601 aagaaaaaag gtcacccagc taaacaaatt tttcaagcta ttcgtattga agtcaatgac
    21661 gaactaggag ctgcagatga atctatacaa caagcaatgg acttattagc tgttgatggt
    21721 cgtatatctg tcatcacttt tcattcttta gaagatcgtc ttactaagca actttttaaa
    21781 gaagcatcaa cagtagaagt acctaaaggt cttcctttta taccagatga tttacaacct
    21841 aagatggagc tcgttaatcg taaacccatc ttaccaagtc aagaagaact agaagcgaac
    21901 aatcgtgccc attcagcgaa attgagagta gcgcgtcgta ttcgataata agaaagaaac
    21961 ttatgacaaa tgaaaaacga actgaagcag taacacaaac cttacaacgt cacatcaaga
    22021 ctttctctcg cattgagaaa gctttttatg gcgctattgt aattactgct attatcatgg
    22081 cagtcggtat tatttacttg caaagtaata gtttacaagt taaacaagaa gtaaatcaat
    22141 taaatagtaa aatcaacgat aaacagacag aatttgacaa tgctaaacaa gaagtaaatg
    22201 agctttctaa tcgtgatcga attactaaaa ttgctaaaga tgctggttta acaattcaaa
    22261 acgacaatat ttatcggaag gtggactagt ctgtgacttt ttttaaaaag ctaaaaaaaa
    22321 tcttccttga ttatgtgatc catataagag ataggcgttc gccgcagaaa aatagggaaa
    22381 gagtaggtca aaatctgatg attttaacca ttttcctatt tttcattttt atcatcaact
    22441 ttgtcatcat cgttggtaca gatagtaaat ttggggttaa tttatccaag gaagccaaga
    22501 aagtctatca acaaagtatg acagtacagg cgaagcgtgg gacaatttac gaccgtaatg
    22561 gaaatcctat cgctgaggat gctacgacct atagtctcta cgctattatc tcaaagaact
    22621 atacgacagc tacaggtcaa aaactatatg tacaaccttc tcagtatgaa aaagtagcta
    22681 gtatcttaga gaataaattg ggcatgaaga aaaatcttgt cctaaaacaa ctcaatcaaa
    22741 agaaattgtt tcaagtttcc tttggaagtt caggttcagg attgtcttat accaagatgg
    22801 ctgacatcaa gaaaacgatg gaaaagtcag atatcaaagg aattggtttt tctaccagtc
    22861 cggggcgtat ttatccaaat gggatatttg cttcacaatt tataggattt actcttcctc
    22921 aggatgatgg agatggaaaa aaattagttg gtaatacagg ccttgaagcg gcacttaaca
    22981 aggtattatc aggtactgat ggcaaagtaa catacgaaaa agaccgtagt gggaatgttc
    23041 ttttaggtac tgcaacaaca gaacgtaggg cggttaatgg taaggatatc tacacaactt
    23101 tatcagagcc tattcagact gttttagaga ctcagatgga tgttttcgca gaaaaaacca
    23161 aaggaaaatt tgcgagtgct acagttgtca acgctaaaac gggagaaatt ctagcaacga
    23221 gtcagcgtcc tacttataat ccttctaccc ttaaaggtta cgataagaaa aatttgggta
    23281 cctataacac actactatat gataattttt ttgaacctgg ttcaacaatg aaggtaatga
    23341 ctctagcctc tgcaattgat tctaagcatt ttaactctac tgaagtgtat aacagtgcac
    23401 aatataaaat tgcagatgcg gttattcgag attgggatgt caatgaaggg ttatcatctg
    23461 gtagttatat gacttttcca caagggtttg cacattccag taacgttggt atggtaacct
    23521 tggaacaaaa aatgggacga gataaatggt taaactatct ttctaagttt aaatttggtt
    23581 acccaacacg ttttgggatg cttcatgaaa gtggaggttt gttcccatct gacaatgaag
    23641 taactatcgc tatgagctca tttggtcaag gtattggtgt aacgcaagtt caaatgttgc
    23701 gcgcatttac gtctatctcg aatgatggtg ttatgttaca accacagttt attagcagta
    23761 tttatgatcc aaatacaggg acttctcgta cagcacgaaa agaagtcgtt ggaaaaccag
    23821 tttccaaaga agcagctagt aaaacacgtg attatatggt tactgttggg acagatcctt
    23881 attatggtac attatacgca gcaggtgccc cagttattca ggtggggaat caatcagttg
    23941 cagtaaaatc tggtactgcc caaatcgccc aggaaggtgg tggtggttat ctgcaaggaa
    24001 aaaatgatac tattaactca gttgtggcta tggtgccatc ggaaaatcct gacttcatca
    24061 tgtatgttac catccaacaa cccgaaaaat ttagtatcac attctggaaa gatgttgtta
    24121 atcctgtttt agaacaagca acagcaatga aagaaacgat attaaaaccg gtattgaatg
    24181 actcagaaca tcagacaaaa tataaactat caaaaatagt tggtgaaaat ccaggtcatg
    24241 tagcggaaga attacgtcgt aatctggttc agccaattat tttaggaaat ggtagtaaag
    24301 tttctaaagt atctaaaaga ccaggagcta atcttgctga gaatgaacag ctactagtat
    24361 taacaaataa actgactgaa ttacctgata tgtatggttg gtctaaagca aacgttgaac
    24421 agtttgctaa atggacaggt ataaaagtga cctataaagg ttcaacctca ggtaaagtaa
    24481 gaaaacaaag tattgatgtt ggaaaatcca tcaataaaat taaaaaaatc aaaattacaa
    24541 taggagatta acatgttttt aagtataatg gcaggtgtca tagcatttgt cctgacagtt
    24601 attgccattc cacgcttcat taagttttac caattgaaga aaattggcgg gcaacaaatg
    24661 catgaagatg tcaaacaaca tctagccaaa gcaggtacgc cgacaatggg aggaacggta
    24721 tttttggttg ttgctcttct tgtctcactt atttttagta ttattttatc taaagaaaat
    24781 agtggtaatt taggtgcaac ttttggtatt ttatctgttg ttctcatcta tggaattatt
    24841 ggttttttag atgatttttt gaaaattttt aaacaaatca atgagggctt gactccaaag
    24901 caaaaaatgt cgctccaact aattgcgggt ctaatttttt attttgtcca tgtgttacca
    24961 agtggcacat ctgcgataaa tatttttggt tttaaccttg aagtaggata cttatacgct
    25021 ttttttgttc ttttctgggt tgttggtttt tcaaatgctg tgaacctaac agatggaatt
    25081 gatggtctag cttctatttc ggttgttatc agtttgatta cttatggaat cattgcttat
    25141 aatcaaacgc aatttgatat tttattgatt atcgttatca tgattggtgc tttacttggc
    25201 ttttttgtct ttaatcataa accagctaaa gtctttatgg gagatgttgg ttcacttgca
    25261 ttgggtgcta tgttagcagc catttcaatt gccttacgtc aggaatggac cttgcttttt
    25321 atcggctttg tctatgtttt tgagacgtct tcagtgatgt tgcaagtagc atactttaag
    25381 tatactaaga agaaaactgg tgttgggaaa cgtatcttcc gcatgacacc atttcatcac
    25441 catttagaat taggtggtgt gtctggcaaa ggtaataaat ggagcgaatg gaaagtagat
    25501 gcttttttat gggctattgg tattttcatg agcgctatta cacttgctat tttatatctt
    25561 taaactttat taggggatgt ggtatggact gccagattcc cttttgcttt cttgtagcct
    25621 ttaatggtat aatgaacata ataatagtaa ggaaatagac atgtcattta aagattttaa
    25681 ttttaaacca tacattcaga gggctctcga tgagttaaaa ttcgtcgatc caactgatgt
    25741 tcaagctaaa ttgattccag ttgttcgttc aggccgcgat cttgtggggg aatcgaaaac
    25801 tggttctggt aaaacccaca cctttttact tcctattttt gagaagctag atgaaagtag
    25861 tgatgatgtt caagttgtta ttactgcacc aagtagagag ttggcaacac aaatttatca
    25921 ggcaactaaa caaattgctg agcattcaga gcaagaaata cgggtggtta actatgttgg
    25981 tggtactgac aaattacgtc aaattgaaaa gttgaaagta agccaacctc atattgtcat
    26041 tggaacccca ggacgtattt atgatttggt gaagtcaggt gatttagtta ttcataaggc
    26101 gcatactttt gtggttgatg aagcagatat gactctggat atgggattcc ttgataccgt
    26161 tgataagatt gcagggtctt tacccaagga tgttcaaatt ttagtctttt cagcaacgat
    26221 tccacaaaaa ttacagccat ttttaaagaa atatttgacc aatccagtaa tggaaaaaat
    26281 caaaacagca acagttatcg ctgataccat cgataactgg cttttgtcca ctaaaggacg
    26341 tgataaaaat gcacaaattt tggaactctc taaactaatg cagccttacc ttgcaatgat
    26401 atttgttaat acgaaagagc gtgcagacga attacactcg tatctttcta gtaatggctt
    26461 aaaagtagct aaaattcatg gtgggattgc acctcgtgaa cgtaaacgta ttatgaatca
    26521 ggttaaaaac ctcgaatttg aatatatcgt tgcaacagat ttagcagcgc gtggtattga
    26581 tattgaaggt gttagccatg ttatcaatga tgccatccct caagacctat ctttctttgt
    26641 ccaccgtgtc ggtcgcacag gccgtaatgg cttatctggt actgctataa ctttatatca
    26701 gccaagtgac gattctgata ttcgtgaact tgaaaaacta ggtattaatt ttattcctaa
    26761 agtcattaaa aacggtgaat ttcaagatac atacgaccgt gatcgtcgta ataaccgtga
    26821 aaagtcctat caaaaattag atacagaaat gattggactt gtgaaaaaga aaaagaaaaa
    26881 aataaaacca ggttacaaga aaaaaattca atggaaagtt gaagaaaagc gtcgcaaaga
    26941 gcgtagagca agtaatcgtg ctaaggggcg tgcggagcgc aaagctaaaa aacaaagttt
    27001 ttaaaattat taaagtctga aatcagttcg tttcaggctt ttttattgat ataaaatctt
    27061 tatcacctga taaggaaata ctattataca gatagatttt agagtgttag actagtaata
    27121 tgatatttta ggagaattct tatgtttaaa aaaataatat tatctattgc aactatagcc
    27181 gcaaccgcca gcctagcagt atcagttcag gcgtcagaga aagtagaact taaagtagct
    27241 acagattctg acacggcacc atttacttat caaaaagacg ggaaattcaa aggttatgac
    27301 gttgatgttg tcaaagctgt ttttaaaggt agtaagtaca aagtaacctt caagacagtt
    27361 ccttttgata ctatttcaac aggtattgat gcagggaaat ttgatttatc agctaatgat
    27421 ttttcatata ataaagaaag agcagaaaaa tatctcttct cagatcctat atcccgttca
    27481 aattatgccg tagtagggaa gaaggggagc cattacaaat cattaagtga cctctctgga
    27541 aaatcaacag aagttttatc tggcgttaac tatgcacagg ttctagaaaa ttggaataaa
    27601 aatcatccta ataaaaaacc aataaaaatc aaatatgttt ctgggacaac tggtgttact
    27661 agcagattaa aaaatattga gagtggaaaa attgacttta tcctatatga tgccatttca
    27721 tctgactata ttgtaaaaga tcaatcatta aacttaagcg tttctccttt gaaaggtaaa
    27781 attggtaata ataaggatgg attagaatac ctccttttac caaaagataa aaaaggtaaa
    27841 actctacaga aatttataaa taagcgtatt aaagttttga aagaagatgg tactttggca
    27901 cgtttaagta aacaatattt cggtggagat tacgtttcaa acattgataa ataaatcagt
    27961 gagactataa gttttatctt tttacatgtg agagtttgcg tgatagaata acgaagataa
    28021 gaaagtttag aaaaggaaag tagaaggaat gaatcaattt atcttgacgg gtggatggtc
    28081 ttggtataat aatttggtta gtcaggttcc agcggggaaa ttatttagtt ggaaggctgt
    28141 tttagatgca ataccctcaa ttttagagcg attacccata acgctattat tgacagtagc
    28201 aggagcctta tttggactca ttttagcttt gatttttgca gtagtgaaaa ttaaccgtgt
    28261 caaaatttta tatccgattc aagctttatt tgttagtttt ttacgtggga ctccaatact
    28321 tgttcaatta atgctgagtt attatggaat tccacttttc ttgaaatttt taaaccaaaa
    28381 atatggtttt gattggaaca ttaatgctat accggcttct gtatttgcta ttactgcatt
    28441 tgcttttaat gaagcggctt atacaagtga aactatacga gcggcgattt tgtcggttga
    28501 tcaaggtgaa attgaggcag cacgtagttt agggatgacc tcagcgcaag tttatcgtcg
    28561 tgttattatt ccaaacgcag cagtagtcgc aacacctact ttgataaaca cgttaattgg
    28621 tctaactaag ggaacctctt tagcttttaa tgctggtatt gttgaaatgt ttgcccaagc
    28681 acaaattatg gatggtagcg attatcgcta ttttgaacgt tatatctctg tggcacttgt
    28741 ttactgggcg gttagtttct taattgaaca attgggaaat gctattgaac gaaaaatggc
    28801 aattaaggct ccacaccatt taactgatga gattccagga ggagtacgct aatgataaaa
    28861 ttgagacaat tgactaaatc attttctggt caaaaagtgt tagacaaact tgatttagat
    28921 attgaaaaag gtcaagttgt tgctctagta ggtgcttcag gtgcaggaaa atcaaccttt
    28981 cttcgtagta tgaattattt agaagagcct gattatggaa caattgaaat tgatgatttt
    29041 aaggttgatt ttaaatcaat cagtaaagat gacatactaa cattacgccg taagttagcc
    29101 atggtttttc aacaattcaa tctatttgag cgtcgtacag ctttggataa tgtaaaagaa
    29161 ggtttgaaaa ttgtcaaaaa gatgtcagat caagaggcaa cacggattgc aagagacgaa
    29221 ctggcaaagg tcggacttgc agatcgtgaa aaatattatc caagacattt atcaggtgga
    29281 cagaaacaac gtgttgcttt ggcacgcgca ttagccatga aacctgatgt tcttctttta
    29341 gacgaaccga catcagcact tgatccagaa ttagttggag aagttgaaaa atctattgca
    29401 gatgctgcta agcaggggca aaccatggtg cttgttagtc atgatatgaa ttttgtttac
    29461 caagtagcag ataaagtttt atttttggaa aaaggtcgta ttttagagtc tgggacgcct
    29521 gaacaattat ttaaccatcc tttagaagaa cgtacaaaag aattcttcgc tagttataac
    29581 aaatcgtacc tataagctaa aatattttga tataatgaag gttagaatgt ctaatcttca
    29641 tttttaatta ttcttgagtt aggctaagta tataagaaag ctgcgatatg ttttgacaat
    29701 cgcttgttgg atagagatgt ttttagaaat agcaaaagtt tatttacaag gtttagggct
    29761 tgccagcata actgttattt tagttggtat attttgggtc ttttggcgtg ccaagaaaaa
    29821 catagataaa acagctagag aacgccaatc tgttctttat gatgtccttc tgatagctct
    29881 tatgacagct ccaatacttg cctttgcatt tgtagctatc attttaatga taaaagctta
    29941 gtcagattag ttggaaatat agacgctttg ctatagaata aaaccataag attcaaagga
    30001 gaaatagtaa tgtatgatac tttaataata ggttcaggtc ctggtggtat gacagcagcg
    30061 ctttacgctg cacgttcaaa ccttaaagtt gggttaattg aacaaggtgc tccaggtgga
    30121 cagatgaata atactgctga aattgagaac tatccaggct acgatcatat ctcaggtcca
    30181 gaattatcaa tgaagatgta tgagccgttg gaaaaatttg aagtagagca tatttatggt
    30241 atagttcaaa gagttgagaa cgatggtgat gtaaagcgtg ttatcaccga agatgaatcg
    30301 tatgaagcca aaacagtaat ccttgctacc ggagctaaaa acagcctttt aggagttccg
    30361 ggtgaagagg aatatactag tcgtggcgtt tcttactgtg cggtttgtga tggtgcattt
    30421 ttccgtgatc aggatctttt agttgtgggt ggcggtgatt ctgctgttga agaagctgtc
    30481 ttcttaactc aatttgctaa aagtgttact attattcacc gacgtgatca attgagagct
    30541 caaaaagtac ttcaagaccg agcttttgct aacgaaaaaa taaaatttgt atgggattca
    30601 gtggtcaaag agattaaagg aaatgagatt aaagtatctg gtgtaactgt tgaaaatcta
    30661 aagactggag aaatctcaga gatgactttc ggaggtgttt tcatttacgt tggtttgaaa
    30721 ccacattcta gcatggtctc agaattaggt attacggacg agactggctg ggtcttaact
    30781 gatacaaata tgaaaacatc aattccagga ctatatgcta ttggggatgt tcgtcaaaaa
    30841 gatctacgtc agattgcgac tgctgttggt gaaggtgcta tcgctggaca gggtgtttat
    30901 aactatataa ctgagcattt ctaagaaata ctaaaagtag aaaagactat cgaaaaacga
    30961 ttagtctttc tttttatttt catgaaagta aggtgataaa ctgaactacc gagatagaaa
    31021 tatgctataa tgttagacat atagccttat cctaggctca tgattaaaag gagattgtct
    31081 gatgtataaa gacgatagtt taacactaca tactgattta tatcaaatta atatgatgca
    31141 agtctatttt aataaaggta ttcacaataa aaaagcggtg tttgaagctt actttcgcaa
    31201 ggttccattt gaaaatggtt atgctgtttt tgcaggttta gaacgtattg ttcgttattt
    31261 agaaaatctg agcttttctg atagtgattt atcctattta gaagagttag gttatcctga
    31321 agaattttta gattacttaa aaaatttaaa aatggaactg actgtcaaat cagctaagga
    31381 aggtgacctt gtatttgcta atgaaccatt agttcaaatt gaaggtcctt tagcacaatg
    31441 tcagttagta gagacagcga ttttaaatat catcaactac cagacacttg ttgctacaaa
    31501 agcagctcgt attcgttctg ttattgaaga tgaaccgcta ttagagtttg ggacacgacg
    31561 tgctcaagaa atggatgcag ccatttgggg tactagggca gcgattattg gtggtgctaa
    31621 cgcaacgagt aatgttcgtg ctggtaaaat ctttaatatt ccagtatcag gaacgcatgc
    31681 acatgcttta gttcaaactt atggcgatga ttaccaggca tttaaagctt acgcagaaac
    31741 tcataaagat tgcgtctttt tagtggatac ttatgatacc cttcgtgtag gtgtgcctaa
    31801 tgctattcga gttgcaaaag agatgggcga aaagattaat ttcttgggag ttcgccttga
    31861 ttcaggtgat ttagcttatt tgtctaagaa agttcgtcag cagttagatg acgccggttt
    31921 tcctaatgca aagatttatg cttcgaatga tctagatgaa aatacaatcc tcaacctaaa
    31981 aatgcaaaaa gctaaaattg atgtttgggg tgtcggaaca aaattaatta cagcctatga
    32041 tcagcctgcg ctaggtgctg tttataaaat tgtttctatt gagactgacg ccggtagtat
    32101 gcgagataca attaaactat caaacaatgc ggaaaaagta tcaacacctg ggaaaaagca
    32161 agtttggcgt atcaccagtc gagctaaagg gaaatctgag ggtgattaca tcacatttgc
    32221 agacactgat gtgacacaat tagatgaaat tgacatgttt catccaactt atacttatat
    32281 caataaaaca gtccgtgatt ttgacgcagt acccttactg gtagatatct ttgacaaagg
    32341 gaaattagtt tatcaattac caagccttca agaaattcaa gagtacggac gcaaagaatt
    32401 tgatcagctt tgggatgagt ataaacgtgt tctgaatcca caggattacc cagttgattt
    32461 ggcacgcgat gtttggcaaa ataaaatgga cctgattgat cgtatccgta aagaagcttt
    32521 ggcaaaagga gaagtaagat gactttgcaa gatcaaatta tcaaagaact cggtgtaaaa
    32581 cctgttatta atccaagtca agaaatccgt cgttccgtag aatttttaaa agactatcta
    32641 ttaaaacata gttttctaaa gacctatgta ctgggtattt caggggggca agattcaact
    32701 ttagcaggac gtctagctca actagcagtt gaagagttgc gtgctgatac aggggaaaac
    32761 tatcaattta tagctattcg tttaccatat ggtattcagg ccgatgaaga agatgcacaa
    32821 aaagcattag attttatcaa gccagatatt gctttgacca ttaatattaa agaagctgtt
    32881 gatggccaag ttagagctct taatgcagca ggagtagaga ttacagactt taataaagga
    32941 aatattaaag ctcgtcaaag aatgattagt caatatgcag ttgcaggtca atatgctgga
    33001 gcggttattg gtactgatca tgctgcagaa aatattacag gttttttcac caaatttggt
    33061 gatggaggtg cggacttatt acctctcttt agacttaata aaagtcaggg aaaacagtta
    33121 ttagctgaac ttggtgctga taaagacttg tacgaaaaga ttccaaccgc agatttagaa
    33181 gaaaataaac caggaatcgc tgatgaaata gcacttggag taacctacca agaaattgat
    33241 gcttatcttg aagggaaggt agtttcggac aagtctcgag gtatcattga aaattggtgg
    33301 tataagggtc aacataaacg tcatcttcca atcacaattt ttgatgattt ctggaaataa
    33361 gaaaaatagc tagaatattc tagctatttt tgtttctcta ataaattact ttcagacttg
    33421 cagaaatatt cagaatgttt ataatagtag ctaagtataa aaggaggaat ttatgtcaga
    33481 attaactcaa acatttacag ataagttatt agctgattac caagcaaata caaaatttag
    33541 cgctattgaa aatgcagtaa ctcacaatgg tttattgaag tctctagaga ctcgtcaaag
    33601 tgagattgag aacgactatg tcttttcaat tgatttgacc aaagatgaag tctctaatca
    33661 gaaacaatca ggacgttgct ggatgtttgc tgcactgaac acatttcgtc ataaactgat
    33721 ttcagatttt aaactcgaga actttgaatt atctcaggca catacatttt tctgggataa
    33781 gtatgaaaaa tcaaattggt ttatggaaca aattattgcg actgcaaatc aggaattgag
    33841 tagtcgtaag gtaaaattcc tattagatgt acctcaacaa gatggcggac aatgggacat
    33901 ggtggttgct ctgtttgaga aatatggtgt cgttcctaaa acagtttatc ctgaatctgt
    33961 ttcatcaagt gctagtcgtg aactaaatca atatttgaat aaacttttac gccaagatgc
    34021 acagattcta cgagaattaa tagcgcaggg agcagatggg gcaactgttc aaaataaaaa
    34081 ggaagaattg cttcaagaaa tttttaactt tttagcaatg aaccttggct tgccaccgca
    34141 atcttttgat tttgcttatc gtgataaaga caatcattat cagtctgata aaaatattac
    34201 accgaaagca ttttatcaaa aatatgttaa tcttgacata tcagattatg tttctattat
    34261 caatgctcca actgtagata agccttatgg acaatcctat acagttgaga tgcttggaaa
    34321 tgttgtcgga ggcccagctg ttaaatattt aaaccttgat atgaaacggt ttaaagagtt
    34381 agcgattgcg caaatgaaat caggagaaac tgtgtggttt ggttcagacg taggtcaagt
    34441 atcaaatcgt caaaagggta tcctagcaac tacgacttat gattttaatt catctatgga
    34501 tattaaactt agccaagaca aagcgggccg cctagattat agtgaaagtt taatgacaca
    34561 tgctatggtc ttaacgggag tagaccttga tgaatctgga caacctttaa aatggaaagt
    34621 tgagaactct tggggagaga aagttggtaa ggatggttat tttgtagctt cagatgcttg
    34681 gatggatgaa tatacttacc aaatagtcgt tcgtaaagaa ttgcttacta aagaagaatt
    34741 agaggcttac aacgcagagc caattacttt agcgccgtgg gatccgatgg gagcactagc
    34801 taattagtaa tagtaattat aaattaaaaa actggatata ttatccagtt tttttaatta
    34861 ccgttaggtg ctgtattgtt attaccatta ccatgtccag ttcctggcac agtttgattg
    34921 gaattattag gggtagtagc attaccatta ttaggtgttt cgacttgtgg ggatgatgaa
    34981 ttggcatcat ctgctgtatt cttatcctct ttttgtttct cagaggatgt tgaactactg
    35041 ctttgacttg actgtccaga attttgatag atattcttat agacagaaga agagtaagtg
    35101 ccggttgtag ttgtcccatt gatatacaag tagcttccac ttcgatagag cccttctggc
    35161 attgtccaat ctgcactgta tccgccagtt aggtaggaca tcattgcacg ataaacctca
    35221 gtagcaatat ccagttggct accataaagt ggtgtaaggc gatttttata accagtccaa
    35281 attgccattg tgtacttaga agtatagccg acaaagtttt cgtcaggagc cattgtacca
    35341 acggcgctat tgtagatacc agtagttgct tcaattttag ctaactcatc ttctgtatag
    35401 ttggaagtac cagtcttacc agcttgtgca acaccaggga tagctgcttt agtaccagta
    35461 ccaaatgtta gtactgtttt caacatatcc gtcatcatgt aggcagtagt ctctttcatc
    35521 gcacggctac cagacgctgc ataagtatca ttggttccat cgctaaattc aattttatta
    35581 acatattgcg gcttatagta agttccgccg tttgcaaaag ccgagtaagc cgctgccatt
    35641 ttctcactac ttgcaccata ttttgcatca ctgctattgt tgttacttga aatagcattt
    35701 gaatagttca tttctggata gtatatgcct aatttttcaa ggaaagattt tgcttcgtct
    35761 aatccagcgg cttcaagtgc tctgacagca gggacgttac gtgattgttg aatagcggtc
    35821 tgcatactca tccaacccat atattgacga tcccagtcat atagttgagt agaagtacca
    35881 ggccagtagt aaactgagtc gtttaatgat tgacctgttg aattatagac accactatca
    35941 atagcaggtg cataagctga gataggtttc attgtagaac cccaatcgcg gtctgttaag
    36001 acagattgat ttgtcccaaa tgaaatattt tcattctgat gacgcccgcc taattgtgca
    36061 atgactttac cattagtcgc atccatgatg gtagatgcta tttgtaattc attgtttgga
    36121 taagcgatgt aagtatcact gttgtagatg tcatatagtt gtttttgtgc atcagtgttg
    36181 atattagtat acacttttag cccagcagta aagatatctt taccagtttt ttgtttaact
    36241 tcactaataa cttgttttaa gtagttatcc atatattttg gataagtaga tttttgcttt
    36301 aattctttta agccatcagt tactggagtt gcaacagctt ggtcgtattc ctttttagaa
    36361 atgtttttat cttgatacat ctgctgaaga acggtattac gtcttgtttg agcagattct
    36421 gggtttttat aagggtcata ttgtgtaggt gcttgaggaa taccagcgag caaagcaagt
    36481 tgtgcaatag ataattcctt taggtcttta ccaaagtatg atttagctgt tgttctcata
    36541 ccataattcc catttcccat ataaacttta ttaatataga aagtaagaat ttcttctttg
    36601 gtgtatttac gctccatttg aagcgcaagc caaacttcct gtgatttacg ttttaacgtt
    36661 tggtcagatt tattggtaga aaagtaagcc agtttaatca actgttgatc aagggttgaa
    36721 ccaccttgcg tattactact aacaaggtta tgccaagctg cacctaaaat acgataaata
    36781 tcgacacctc tatgtttaaa gaaacgttta tcttctatag aagtgatagc gttaaccaaa
    36841 tttagtggaa tgctatccgc actaacactt tcacgctttt ctgagcctaa atcggcaata
    36901 agtttattat taccatcata aaccaaacta gagttaacgg attttaaagc ttgatcggtt
    36961 aatttcggag aacgactaac gtagtatgca aataggagcc cacctattac aatagctaag
    37021 ataataaatc ccattataat gccaaaagca tacttcaata gtttgataac agattccttt
    37081 ttaattgtaa tcaccgccta ataatttttc ttcaataata tctaagtaag ggacttgagg
    37141 aaaggcactc tcctttacga aaaatccatt ttttctgata taatcaatag gcattgattt
    37201 attgccttta tcaatctgat aaaatgaaat taactcatta gcagggagta gataggtttc
    37261 cttaagtgtg gaaaaatgaa gcaagacaaa gcaaatccct ttttgctgta atacatttgc
    37321 catgtgctct atttggtgag catgaaaatt tttcataggc atagcagttt tctgccgggt
    37381 ttctttggct tcaaagtcaa tatagtaacc tttgtaaaca ccagaataat cagtagtaga
    37441 agcttgtcta aaataagctt ctacaatctt ggcacgacta cgtttaggat aatctacctt
    37501 gacgatttgt actggcgttg gtttcttatg aatgacagct aattcgtgag acaaatagta
    37561 gtcattggtt gcattgatag cagcttcaaa tgacatacca cggttggcaa aatcgatttt
    37621 ctttttcttt gattttgtaa ctgttgtttt ccgaataagc tgatgtggat agttaaccat
    37681 ttttctcctt gcattacaaa taaaatgcta agataagcaa aactattata ccataaaatt
    37741 aggatggaca acaaatgaag tcaactattc ttgtaacagg ttacaaaaat tttgaattag
    37801 ggattttcca ggataaggat cctcggatta ctattataaa aaaggctatt gataaggatt
    37861 ttagacgctt cttggaaaat ggagcagact ggtttatttt tatgggaaat ttagggtttg
    37921 agtactgggc tttggaagtt gctcttgatt tacaaaaaga atatgatttt caaattgcga
    37981 caatttttac ctttgagaac catggccaaa attggaatga ggctaataaa gctaagttgg
    38041 cactctttaa gcaagttgac tttgttaaat atactttccc aagctatgaa aacccgggtc
    38101 aatttaaaca atacaatcat tttctcatta acaacacaca aggtgcatat cttttttatg
    38161 attctgaaaa tgaaactaat ttaaaatttt tgctagaaat gatggaaaaa aaggaagctt
    38221 atgatatttc tttcctaact tttgatcgct taaatgaaat atatgaggaa tgatcgtttt
    38281 ttcggatatg agaacgtttt acgcttgatt tttccattta atttaggtat tatttacttt
    38341 gatataaagg ataggactta tattaaaacg atttgactgg tttggagaat agaatggcaa
    38401 gtattattta tagcccaaaa gatatttttg agcaagattt taaagttagt atgcggggat
    38461 atgacaagaa agaagtcgat gtatttctgg atgatgtcat caaagattac gaaaattatc
    38521 ttgagcaaat tgagaagctc caaatggaaa atagacgtct acaacaagct ttagacaaaa
    38581 aagaaagcga agcttcaaat gtaagaaatt cagggacagc tatgtataat caaaaaccga
    38641 ttgcccaatc tgcaaccaat tttgatattt taaaacgcat tagccgcctt gagaaagaag
    38701 tttttggaag acaaattaga gaataaggat ttctcaaata tgcaattttt ggataatcgc
    38761 gtagtattga tatactatga ggaaagtcca tgctagcact ggctgtgatg ccagtagtgt
    38821 ttgtgctagg cgaaaaaata agcctaggga gatagctagc tatcttacgg caagcaaaag
    38881 ggctaagtct ttggatatgc ctgaatagct ttgaaagtgc cacagtgacg tagtttctag
    38941 ggaaatctag aaagtggaac gcggtaaacc cctcaagcta gcaacccaaa ctttggtagg
    39001 ggcatgggat agttggaatg agaacaatct atcctgactg ctttgcagta gacagatgat
    39061 tatcgaaaga aacaattcct agttgtttct ggaacaaaac atggcttata gaaaattgca
    39121 taataggtta gtggctgaga taacatctca gccaattttt aactgtgatg aatacgatag
    39181 tgtaaaagag gaaaaatgaa agaatcattc aaattaatag caactgctgc tgctggttta
    39241 gaagccattg ttggtagaga aatcagaaac ctaggaattg actgccaagt tgagaacggt
    39301 agagtccgtt tccacggaga catcaaaaca atcattgaaa cgaacctatg gctacgagct
    39361 gccgatcgta taaagattat tgtaggagag tttccagcac gtacttttga ggaacttttt
    39421 cagggtgtat atggccttga ctgggaaaat tatttaccgt taggggcaaa atttcctatt
    39481 gctaaagcaa agtgtgtcaa gtcaaaattg cacaatgaac ctagtgtaca agccatttct
    39541 aaaaaagctg ttgctaagaa acttcaaaaa gtctttcacc gtcctgaagg agttccctta
    39601 caagaaaatg gagctgaatt taaaatagaa gtttctattt taaaagataa agcgactgtc
    39661 atgattgata cgactggatc aagtctcttc aaacgtggtt accgagcaga aaaaggtggt
    39721 gcgccaatta aagaaaacat ggcagcagct attattcaat tatctaattg gttccctgat
    39781 aagccattga ttgatccaac ttgtggttca ggtacttttt gtattgaagc cgctatgatt
    39841 ggaatgaata tagctcctgg ttttaatcgt gattttgcct ttgaggcgtg gccatgggta
    39901 gatcaaagtc aagtacaaaa ggttcgcgat gaagctgaga gtaaagcaaa ctatgatatt
    39961 gatttagata tttcaggttt tgatttagac ggtcgtatgg tagaaattgc tagaaaaaat
    40021 gcagaagaag caggacttgg agatgttatc aaattgaaac aaatgcgtct tcaagattta
    40081 aaaacagata aaattaacgg tgttattatc tccaacccac catatggtga acgactcctg
    40141 gatgacaaag cagtggacat tttgtataat gagatgggac aaacatttgc gcctttaaaa
    40201 acttggagca agtttatcct aactagtgac gaaggatttg aaaaaaaata cggctcacaa
    40261 gctgataaaa aacgtaagct ttacaatggt actttaaaag ttgatttgta ccaatattac
    40321 ggtgaacgtg tgagacgtca agtaaaataa cggggagaac tcatgtcaga agatcaaaaa
    40381 cacccatttt ttgaaccaga aaaagaaact gatggtcttg aatttaaaga tgctaaagaa
    40441 atgacagtag aagaggctgt tagaaaagat tctgaaatta aggctggtat taccgaagaa
    40501 gatagtattc ttgataaata catcaaacaa catcgtgatg aagtagcttc acaaaagttt
    40561 gagacaaaat caagcgattt tgcaaacctt gatacggcat ctttagatga ttttatcaag
    40621 aaacagcgcg aagaattatc agcgatgtta gctgcagaag agctttctaa aaagttggat
    40681 aattcagtct ctcaagaaca agatatagaa gctaatgctg tctcacctaa agaagaatct
    40741 tcacaagaac aagaaaatag tgtaactcca gttcctcctc tgaatacgga agcagaacca
    40801 actgcaacag aaccagattc gactattgct gattcagagg agtataaatc ctcttctaag
    40861 aagcgtggag gtattgtagg aacattgatt gctcttattt tacttctaat agtaacaatc
    40921 tttggttaca actatttcaa gaataataat tcgacaaatt ctcaaactgc aacaagtcaa
    40981 agttctagtt ctaaggctac gacgacttca tcagaagaag ataaaaaagc tagtcagaac
    41041 ttagataatt tcaacaaagc ttatgctaac ttttttgttg atgataagaa aacccaactc
    41101 aaaaatagcg aatttgataa attgtcagaa ttagaaaaga aagttgatgc tttaaaagga
    41161 actaaatact acggtaaagt aaaagttaaa tttgatagtc taaaacgtca aattgatgct
    41221 gttaaagctg ttaacgacaa gtttaagtcg cctgctgtag ttgatggtaa gaagagtgaa
    41281 aaattagaag taaaagatgg agcgaatttt gactctttag atagtaaaac tcttaatact
    41341 ggtaatgcta gtctagatag tttacttcac tccatcgttt caactggtcg taatcaggta
    41401 aaacaatccg aagaacaggc taaaaaaact caagaagaac agtcttcgtc taataaggtt
    41461 tcagacactc aaacaactga acaaccaaac gtaactaacg gtcaatcttc ctcatcagct
    41521 gctacaataa ataatcaggc agctggcact gcatcaggta atttggaacg taaccgtagc
    41581 cgagtaccgt ataataatgc tgctattgcg gacacaggaa atcctgcctg gatatttaac
    41641 ccaggagtat tagagaaaat cgtggccact tcacaggcgc gaggctattt ctctggaaat
    41701 aactatattc ttgaacctgt aaatattatc aatggtaatg gttattacaa tatgtttaaa
    41761 ttagatggca cttatttgtt ctctatca
//
LOCUS       DATROQ010000010        36644 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.010, whole genome shotgun sequence.
ACCESSION   DATROQ010000010 DATROQ010000000
VERSION     DATROQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36644
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.010"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            1..1626
                     /locus_tag="VBC94_000456"
     CDS             1..1626
                     /locus_tag="VBC94_000456"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEO6978753.1"
                     /translation="MAEIVEQVGIGFDDIELYGKYKAKLSFDKIEAVRSQKVGKLILV
                     TAINPTPAGEGKSTMSIGLADALNKIGKKTMIALREPSLGPVMGIKGGAAGGGYAQVL
                     PMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELDIDQRRVIWKRVVDLNDRALR
                     QVIVGLGSPVNGIPREDGFDITVASEIMAILCLATDLSDLKKRLSNIVVAYSRDRKPI
                     YVKDLKIEGALTLILKDAIKPNLVQTIYGTPALVHGGPFANIAHGCNSVLATSTALRL
                     ADYVVTEAGFGADLGAEKFLDIKTPNLPTSPDAIVIVATLRALKMHGGVSKEDLSQEN
                     VEAVKRGFTNLERHVNNMRQYGVPVVVAINQFTADTESEIATLKTLCSNIDVAVELAS
                     VWEDGADGGLELAQTVANVIETQSSNYKRLYNDEDTIEEKIKKIVTKIYGGNKVHFGP
                     KAQIQLKEFSDNGWDKMPICMAKTQYSFSDNPNLFGAPTDFDITVREFVPKTGAGFIV
                     ALTGDVLTMPGLPKKPAALNMDVLEDGTAVGLF"
     gene            1745..3340
                     /gene="cls"
                     /locus_tag="VBC94_000457"
     CDS             1745..3340
                     /gene="cls"
                     /locus_tag="VBC94_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEO6978754.1"
                     /translation="MIWRGEKLISNKVKIVRLLNKSKKSLLRGIFSRTTVIAILLILQ
                     LLFLLASYSWLEQYRVWLATVEHILTIGAVLYLVNSEMDALSRVTWLILVMIAPLLGA
                     MFLMYTKFDWGYRGLKQRLETLIDESQIYLEDDPETLNQLKSSTSTTYHLVQYFEKAH
                     GNFPVYRNTDVTFLPTGEAFFEKMKEELLKAKKYIFLEFFIIDEGIMWGEILSILEQK
                     VEEGVEVRILYDGMIEITKLSFDYTKRLEKIGIKAKAFSPISPFISTYYNYRDHRKIV
                     VIDGVVGMTGGVNLADEYINHIELFGHWKDSGIMLKGKAVDSFLLLFLQMWSITEEKM
                     LVAPYLGVHDDLVENEGYVIPYGDSPLDTDKVGENVYIDILNHAREYVYIMTPYLILD
                     SELEHAIQFAAERGVDVRIIMPGIPDKPIPYALAKTYYQALIKSGVKIYEYTPGFVHS
                     KIFLSDNTKAVVGTINLDYRSLYHHFECAVYLYKVDAIQDIYRDYMDTLNKSRLVSLK
                     DINNIPKFQKVIGIVTKTIAPLL"
     gene            3514..3885
                     /locus_tag="VBC94_000458"
                     /pseudo
     CDS             3514..3885
                     /locus_tag="VBC94_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282473.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3896..4072
                     /locus_tag="VBC94_000459"
     CDS             3896..4072
                     /locus_tag="VBC94_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978755.1"
                     /translation="MKSRDDEILYTARLYLFGVIPLWKVKHTKQEWKRCILLQPSNKL
                     IELQFIEAEEEEKE"
     gene            4364..4806
                     /locus_tag="VBC94_000460"
                     /pseudo
     CDS             4364..4806
                     /locus_tag="VBC94_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447421.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4913..5077
                     /locus_tag="VBC94_000461"
     CDS             4913..5077
                     /locus_tag="VBC94_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978756.1"
                     /translation="MTKQVQQSRELVLKEIMQSQGVTRTKACSILKELEEFGLFQFSD
                     SGQFMLKVGA"
     gene            5079..6113
                     /locus_tag="VBC94_000462"
     CDS             5079..6113
                     /locus_tag="VBC94_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEO6978757.1"
                     /translation="MKRISARQVQSSERFYRIPKIFVESPFYKSMSTDAKFTYAILKD
                     RFELSLKNNWIDKNGDVYLIYTVTELQEILGYGNKKVIKLKKELQEYGLLEEVRQGLN
                     KPNLLYLGNVDYQNVPTEEIHSKPSSVVEVSKQHFQKNQGNVKMTRQKMSKRHANDTE
                     NSETELSNDTNRYQDEIPLSTLSDSEAFQRGQHGFLSDQTIQRLSLFGKDAKVLENKI
                     YQAKRQVEKDYSHLLNHQEMIYGEVWSLELEREVDKLIFKIKTGEHEGKAIKNISGYF
                     YKMMVRFWKMALLIEKEQGFIALSNQSDYAKSFPEECPSLISYYYPDKLSESKLNQYL
                     SELSERIETC"
     gene            6107..6796
                     /locus_tag="VBC94_000463"
     CDS             6107..6796
                     /locus_tag="VBC94_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978758.1"
                     /translation="MLDKPSRNSPRDKLKNHLKACQTPLKNYPLKAVFLAVIFIAIVF
                     SFFSQILISLKAMGFTTLSLLILLVLGYPCHLQSLYHDLKLSIYQYQQDKIDFFKTYG
                     EKQEDVIDDIRIIYETDNTVTVQFIYQGQPSQLSLSKGAIPQSYANQQLVVIARCRAI
                     AKEHLSAYLTLFEIRDLAQEYQTILKHPIITEGLLSDNDILQLSEAPDKPMVSFQLAL
                     ITQPKESETSK"
     gene            6793..6933
                     /locus_tag="VBC94_000464"
     CDS             6793..6933
                     /locus_tag="VBC94_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978759.1"
                     /translation="MIIKERNGFLTIPKSIFSLPPEEKTQALKELEEDMEQHKAKGGD
                     ST"
     gene            6930..7127
                     /locus_tag="VBC94_000465"
     CDS             6930..7127
                     /locus_tag="VBC94_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6978760.1"
                     /translation="MTTITQNRVKELRKQAGLSQQALADQIGVMRKTISHWELGNHRI
                     STDNAKKLSQFFQVSIDDLLE"
     gene            7510..7809
                     /locus_tag="VBC94_000466"
     CDS             7510..7809
                     /locus_tag="VBC94_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978761.1"
                     /translation="MTDETKKVHLRDLRNLGKQGGATARLEDGTELFLKPGYAVRTAK
                     GYVDGIPRDVEFHLTYRSIFNQIRTIKKDGHLVARKERSPYGLVLQLTGKGYKRP"
     gene            7826..10090
                     /locus_tag="VBC94_000467"
     CDS             7826..10090
                     /locus_tag="VBC94_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEO6978762.1"
                     /translation="MNQIKIITGLTVATLSAVVGNVYAEDITPTAPVNEPQVSSETAK
                     TPQVTESQVNSAKVTVDQATSDVNNQQIVVDEAQKQKDQSQQNLAKATSTVTEASTIA
                     AEATPEVVKEATKAVTEAKEAVTDAEANVADAKKTEQKANQEVQSQAKTVDGNVKVVA
                     DKESEVKQAEGAVTTAQEAIDSKTANTNASEAEKAVTEKQTKLETAETNLTEAQKQDA
                     KIAEEKRLAEQEVVSKQLAVTDTQTLLKKLVTEINNEKVSTSLENQAYFNQRDGAWAR
                     YYGNYTFAATGCVPSSLAMVFTELARRQITPTEIANYLWNNSNEFNKNYGGTSGKGLV
                     QATKHFGFVPTHLASQSAIVEALQAGHHVLAAVQQDKFSPWGIQYSHEIVLRGYSNGN
                     TYVYDPYNRANIGWYPVANLWNEQSRDAIDTSGVGVPFFKITTQKMAQLEAQTAQVQS
                     SLNTAKNQLAKTQDVLRALEATPLKTPEAQVKLNQAKEALALAQADYTKAQEAVKLAS
                     QDLAVKEETLKNAQADLLTKQTALKDAQTVLVASQVKLADLKATLATVENNVKKAQAT
                     LTDAKAIVGQKQAKLLALQNAPKILADAQAKLVTAKNDLANKMAILDEAVAKLKSLQA
                     VQAEAQKQYHVVFEAYKAVRDAKEQAKLAESYNHIIAGGGEAIPVVDETGKITGYVDG
                     SQKAVANEVTLALTSNSQAPLESPVNKENQNVTKSSQALPHTGEAGLSLLSIVGAGII
                     STLGLVSLKKRRTH"
     gene            10110..10832
                     /locus_tag="VBC94_000468"
     CDS             10110..10832
                     /locus_tag="VBC94_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6978763.1"
                     /translation="MSKKQLLLLTCVSTIALAGTTAFAEDVVPVDPAVPSTEVVTPPN
                     PVDPSLPTDTTDPSTPVAPEKLPDTPKPAEPERPKEELPAPVPPTTPSAGKEDKPETV
                     PGQAETPKEEVKPENPKSSEDKKKEELVPDNVSKDIIEKVDTTTGEVVFKPIPVTPDK
                     TVIGTKDSQVIIRDQAGRTQVVTPESLGGHVNPNGTVTLKDATGAEKTLPKTGDKQSV
                     LGYLGMMLISVLGYAVKKKVSH"
     gene            10885..13686
                     /locus_tag="VBC94_000469"
     CDS             10885..13686
                     /locus_tag="VBC94_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6978764.1"
                     /translation="MNSQETKGHGFFRKSKAYGLVCGIALALAAAFTLATSQVSADQV
                     TTQATTQTVTQNQAETVTSTQLDKAVDTAKKAAVAVTTTTAVNHATTTDAQADLANQT
                     QAVKDVTAKAQANTQAIKDATAENAKIDAENKAEAERVAKANKAGQAEVDARNKAGQA
                     AVDARNKAKQQAQDDQKAKIDAENKAESQRVSQLNAQTKAKIDAENKDAQAKADATNA
                     QLQKDYQAKLAEIKSVEAYNAGVRQRNKDAQAKADATNAQLQKDYQAKLALYNQAKKA
                     KEEADKQTINNVAFDIKAQARGVDNKEYGNSIMTAKTKPDGSFEFNHDMIDGVKTIGY
                     GKLTGKVNHHYVANKDGSVTAFVDSVTLYKYEYRNVAQNAAVNQNIVFRVLTKDGRPI
                     FEKAHNGNKTFAETLNKTLQLNLKYELKPHASSGNVEVFKIHDDWVHDTHGSALVSYV
                     NNNDVVPNVVIPKQPTPPSTEKVTPEAEKPVPEKPVEPKFVTPTLKTYTPVKFIPKEY
                     KPVPITPEKFTPEKFNPAQPKVKPHVAIPEKINYSVSVHPVLVPAANPSKAVIDEAGQ
                     SVNGKTVLPNATLDYVAKQSFSQYKDIKASAEAIAKGFAFVDQPNEALAELTVKSIKA
                     SNGDDVSSLLEMRHVLSKDTLDQKLQSLIKEAGISPVGEFYMWTAKDPQAFYKAYVQK
                     GLDITYNLSFKVKKEFTKGQIKNGVAQIDFGNGYTGNIVVNDLTTPEVHKDVLDKQDG
                     KSINNGTVKLGDEVTYKLEGWVVPANRGYDLFEYKFVDQLQQTHDLYLKDKVVAKVAI
                     TLKDGTVIKKGTNLGEYTETVYNKTTGRYELAFKKEFLAKVSRESEFGADDFIVVKRI
                     KAGDVYNTADLYVNGYKVKSETVVTHTPENPRPVTPQKVTPAKGLPQTGEASVAPLTA
                     IGAIILSALGLAGFKKRQK"
     gene            13969..14196
                     /locus_tag="VBC94_000470"
     CDS             13969..14196
                     /locus_tag="VBC94_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978765.1"
                     /translation="METAVRVVTAMGAILAIVGLGWVLTGAFDYFAGRKNGSPQMMDQ
                     GFTSMISGGALAVISAGVAAAIVAAMRAISF"
     gene            14301..14882
                     /locus_tag="VBC94_000471"
     CDS             14301..14882
                     /locus_tag="VBC94_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978766.1"
                     /translation="MSESFDYVAFARDFEKRHGRPPTAEELEKANVSVATVITSDNQK
                     IDFRMVDSLDYVEGYKDKSSFGERLKTGLSFVIRNFFRVLLIVIQTPVYLTLFFFNLI
                     KSTIGVFVIWFVSKFVLGWLVGIIAGLIYGFDLYKNPFPSPIKDIVDFSFGVNFFEDA
                     VPNFFPHPVADAWIIGITIVFLALVMTFSKSET"
     gene            14933..15685
                     /locus_tag="VBC94_000472"
     CDS             14933..15685
                     /locus_tag="VBC94_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978767.1"
                     /translation="MKDILSELTKGLGSYNSSVNNSMTAINKSLMAIGYILVSILFLL
                     ELLSWYRFIRNQGGEMTWKLFLEVAVKYFIAYFLVSQSGAILNAILWFTNGVTKLIGV
                     DLSGDNMFEFVWLKKGNLGVRILVNGLAAIAGVGAMISIKVMVLLRFIELYFYKAIAP
                     VIVAFWMGDDTRHIAKNFLKTFGAIALQSAVIVLLLAIWQGLNIDAVIKISKDSWVGA
                     FAAGFSYIGKCIVFLILLFGSQRKAKQLLQVS"
     gene            15722..16018
                     /locus_tag="VBC94_000473"
     CDS             15722..16018
                     /locus_tag="VBC94_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978768.1"
                     /translation="MNLEKLKQRRDELIRDNEWMDGLIKEKEEKLWELKVRSITASEL
                     ARSAMQSAMRTAGIVDSFYGPSAQSSDNQANKAVEAVHNKSCFFMLERKSDRND"
     gene            16011..16469
                     /locus_tag="VBC94_000474"
     CDS             16011..16469
                     /locus_tag="VBC94_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978769.1"
                     /translation="MINQGQEYQYFKDKISHLEREVSRLSPYEYEHRLLKDVIADCLL
                     QGQITVSELPQAIRLIQGDDLFYTYAWRFVEATGDCQAGITILKILQDDLNYFFAIGK
                     LSQKQYSQWLEKWLSFLERGRIAFKGEKDFERYFQDQKEANRSLFSDFNL"
     gene            16531..16890
                     /locus_tag="VBC94_000475"
     CDS             16531..16890
                     /locus_tag="VBC94_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEO6978770.1"
                     /translation="MNKLGSEFFKAIDQFERGIIGSFTWRQILMFFGIVLGVTLATVI
                     SLLQLPSILFYLSLALTVVPAFIYGIKKDEHLKEVITFRLKVQERAYQTEYESEENYG
                     AFIPQKGVQEWNDPNES"
     gene            16871..19231
                     /locus_tag="VBC94_000476"
     CDS             16871..19231
                     /locus_tag="VBC94_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEO6978771.1"
                     /translation="MTLTKADKERAKQLQKALKASTQNTIQYTSLFEDGLMHIVDEAY
                     SKTWQLGDTNYITGSEDEKLDIIEYYVDALNGLDSENTYQLTILNRPVPTTLLDQITY
                     DLEGDERDVFRKEYNNIIRSRFAKDQNNFKVEKFVTVSTRAKDRKQAYRKLNDVDTHF
                     TTQFQLVDVLLKSLDGTERLNIFADLLRGNPYLDVDYKDVRISGLSTKSFIAPGRLWF
                     QPDQCRMDKKYCKILFARTFPAFLNDRLIKSLTDIGIELSISIHAKPYDVAEAVKKVN
                     TAEAGVKMDMVKSQVAAAQKGISGNLAVSSVAQDTADEAEKWKTEINDNDQKMFSGVF
                     LVMVKADSAEELADYTSRIKQAGRKHVIEFEETYYHQEEALNTMLPIGKTYIDVKRRF
                     MRDMTTTNIATQIPFTNTDLQSQSPLANYYGQNQISNNIISLDRQRDLYTSSGVILGS
                     SGSGKSVFVKINEVIPAILRFPNDRVIIVDPEEEYVDIGRAFGAQVIDIYPGTKTHFN
                     LMDIPDLDKLRDEDKDFVGQKASLIMGLFENILQEVTDDDVSLIDRVTRLCYEQITDR
                     TPTLKDWHDILLEQPEEEAQSLALKSESYTKGSQDIFAYETNVDLNNQVVIFNLKKLS
                     GKLKPFAMMVIQDYIWQHVVDHKDEFVTRVYFDEMQNQFETDDQAAFFSKMYARIRKY
                     GSIPTGITQNVETLLSRKEGRNLLYNSEFLVLLKQKKTTIPYLLQTINLTDALVRYIE
                     KPKAIGTGLIIAGTTTVPFENPIPEDTELFRLVATDAYRKMEEENV"
     gene            19224..19817
                     /locus_tag="VBC94_000477"
     CDS             19224..19817
                     /locus_tag="VBC94_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978772.1"
                     /translation="MFEDVTLQLPKNEEKKLMAHYYQLHQEAIEEALAGKELWKPYIR
                     MSGLSQWLDVSTTTIVKWQREGMPHIVIDGVTLYDKRKIATWLNHFERNKIISQEKQM
                     KIFPGYLSIKHVFGTIITYSKKVVLDLIQDLRDEDIQDFSYWSPLYFLETDELGQPVY
                     LTYLVNEVGIPLYPDCYYYITLTKEQESKLESLELSI"
     gene            19841..20167
                     /locus_tag="VBC94_000478"
     CDS             19841..20167
                     /locus_tag="VBC94_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978773.1"
                     /translation="MSAVPIAAAYLVRKRQEEKKVRQKSEKELKKIKVMVTGEEPVYY
                     GHALKSKMKTEKYSRSPVIGEYALKLSKKHPGKVTWIDIAVLLGLILAGLATIWSVYA
                     VIHDTF"
     gene            20202..22892
                     /locus_tag="VBC94_000479"
     CDS             20202..22892
                     /locus_tag="VBC94_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6978774.1"
                     /translation="MTEEKQDLRSRVVRKRQIKASVHRHNQSSARKAQKAAKGEFQIA
                     TKELKEAREAYQKAQTDFRAGIIDDEALQKAKNRLKQAQNKYKDAKKVKKKVRKTNLT
                     LSQKARQTGRRITKRTGQEILEMGLEQDDTLSDLVRTKHQIRQTRVMAKQAGRATGKS
                     VKLTYRGAKGTVKLGAKGTKYAYRQTRKGSVKATQASLRAASKTAQTLVATSKALVSA
                     ITPIIANPITWVVAGVISLLLLIVILVSSVFSSNVVQQNEFTLNQSWLHISQMDRKKS
                     SDKVDYYTDIDSILLYMNYRYGGEWEPDAKWDDGFGGKVTGALGFNHFSDALDDIWKE
                     ENSDITNLKTMAELYTNGKKWIKFSKDDLEEYKEILDSQVETGKYLAYQELSNPFYSS
                     NDDKADSEYLTVTKRYGYTDKDTIDPTTTLQAKASQKLYAVMDGKVTVTKKDLKGEKT
                     KTTNLIIEGKEARFIYYDISNSRVRTGDRVETGQELATVSGSTQKIAYAKRYGEVDDK
                     DATWLKKIRDREVSYGYTIQTKMNKKEVWVLTNPGFYFPFVKYAETTIVTQTPSEMSG
                     RAKQFYDLVKKYLPQATDNGIAAIVGCFGVESGINPKRAEGDYLSPPVGASDSSWDDE
                     SWLTLGGPAIYGGGYPNILHRGLGLGQWTDTSDGANRNTLLREFAKKEGQKWYSLELQ
                     VKFMLEGDNPYYINTFKRVATNNGDVDSLTAEFLAKWEGVPGNKLAQRQAIAKQALMW
                     FKQPTHSGGASTLQSSWNFPKEYASKLKSYPSAATVSRSLNGNTYPIGQCTWYVYNRL
                     SEAGAPAINWLGNGQDWVRNLVAKGWTFSNKPVAGAVCSTAGGFDYTMPTYGHVSYVE
                     HVNEDGTFLVSECNVMGIQNQIHWAVKLNQSYYTFAIPPK"
     gene            22913..23518
                     /locus_tag="VBC94_000480"
     CDS             22913..23518
                     /locus_tag="VBC94_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978775.1"
                     /translation="MIDTFKVKWFKRGLSLLALLLVFFLGLGFGQMGNSSHSKDRPKQ
                     TATKKTRTNTITQTQVKEFLVAYYTKKDLEENRNRYKDYMTEGLYNATISEEDKAQNK
                     AYKGFVVNFEFQDANIYIDQVHKQVICEVTYTNDLLQKKNNKDGAQIGVVNHTTIQLV
                     YTKLNGKNLINQMSTILLTDSANPSEETESYGAIAPSNTKD"
     gene            23538..23810
                     /locus_tag="VBC94_000481"
     CDS             23538..23810
                     /locus_tag="VBC94_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978776.1"
                     /translation="MINEEHMNQTSSSKSRRQKSTQERLEDKIKQLEKAQESQDALQK
                     KIKTLKDDIAILEGKRQQEIMAEYGIDLQGLEAILATHKEKLGGEG"
     gene            23810..24595
                     /locus_tag="VBC94_000482"
     CDS             23810..24595
                     /locus_tag="VBC94_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978777.1"
                     /translation="MFRIQNQSDGKEQEFQNLAALLYTLEGEEKRCLQLNMRATFYLF
                     HLDKNEEVLESMELTIPSQEDKEVQELIGDFGLKGETKKFFWQRSMDKKPIPIKTSPN
                     NHQELPSKTGALTLLKRLVWLLPMVLSVVSIYLSLETLQLVKTQSQVTPKVETVVTNQ
                     KADVFCRYFISSYFSQSPNLGDYLSEKLSIDDLKTDKVTPVSVLLESQEIKGDTTLVT
                     YVVNIKEAQDHVSSKRLTLAVRADKKAQYGYLVIKKLRLTAYP"
     gene            24617..25108
                     /locus_tag="VBC94_000483"
     CDS             24617..25108
                     /locus_tag="VBC94_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3801 domain-containing protein"
                     /protein_id="HEO6978778.1"
                     /translation="MDQERVMEKGSRVTLETGKHLLQFLAYAVSQGAKIYTEHKQSGE
                     MSWKDFNQLPRTKDHIDFEEAEVNLEKLRKELKKSGVNFHFKKNGDGTKQVWFEAINQ
                     QVIQEALRKINQEIVSNPKEALDKYMKKPDELTPKQQIEKLKKTSKKSTEAVKTKKKG
                     KGI"
     gene            25108..27153
                     /locus_tag="VBC94_000484"
     CDS             25108..27153
                     /locus_tag="VBC94_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEO6978779.1"
                     /translation="MELKRKRLLPYLLLSLLLAYLTHRAYVLYCMAPQADMTNLFAPY
                     TYVFEKITEPPYFYWNTSPLAILSALVGFFVGLLFYLKIRLDGNYRHGEESGSACFAT
                     AQELKGFQDEESRNNMIFSREAQMGLFNKRLPFERQLNKNVLVAGLPGDGKTYTYVKP
                     NLMQMNSSFIVTDPKGLLVREVGTMLKENGYQIKIFDLVNLTNSDMFNPFRYMRSELD
                     IDRITEAIVEGTKKGDREGEDFWNQAKLLLNRALLGYLYFDSQVRHYEPNLSMVADLL
                     RNLKRPNEKELSAVEKMFKQLEEKLPGNYACRQWELFNSNFEAETRTGVLSIVATQYS
                     IFDHDAVTNLIKTDTMEMDTWNTEKTAVFVAISETNKAYSFLASTFFTVVFDQLTHSA
                     DAIIQGEKKGYELKDLLHVQFYFDEFANCGKIPHFNEVLASIRSREMSIKIIIQAISQ
                     LDSLYGVPARKSMVNNCATLLFLGTNDEDTMKYFSMRAGKQTIIQTSYSEQRGHRISG
                     TTSRQAHQRDLMTPDEIARIGVDEALVFISKQNVFRDKKAMVSNHPMKDRLANHYQDK
                     TWYHYKRFMEEGAEFIDAVMTGQIPEDRIWAPDMSDYQAFLEENGFDNTRQSAPETSV
                     HNQVPVTNPEMPSESPSQTPETSTKLVDMDTGEIFELPPEDREGFGEVSFDDQRVEI"
     gene            27195..27560
                     /locus_tag="VBC94_000485"
     CDS             27195..27560
                     /locus_tag="VBC94_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978780.1"
                     /translation="MTPQILRRLDVKKQFIEAIDPFVHRQTLKPKAVNSSKTTMSIQR
                     YNHAGTKIQLRIGYSKVLIRIFSNGKINLTHYDLFFDREETLEITDAFDNGVYTQDEV
                     DGFIKQAKTFIKQALKGEV"
     gene            27562..30801
                     /locus_tag="VBC94_000486"
     CDS             27562..30801
                     /locus_tag="VBC94_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEO6978781.1"
                     /translation="MANTYDLISQRIEAQRQKLIAIDIVAVASSLGLNLKQGSGGHLY
                     WDEHDSFHIYPQTNTFRWWSRSMGTNTIDLVQVIQEELTGKKPNFRETVNFLETGQFE
                     SVTVTPVVREPFKHYLAPYEHHNFDLGRQYLKEERGLSDETIDFALASGSMSSATLKK
                     GDYFEPVIIFKSFAEDGRMIGGSLQGIVENKVQHPERGRLKQIMKHSDGLAGFHLDVG
                     TPKRLVFSEAPIDLLSYYELHKESLQNVRLVAMDGVKKGVISRYTADLLTDGQYSQTM
                     PRESIRGAIDAINQTTRILKNNPNLITIAVDNDEAGRNFIKELQEDGIPITIDLPPRK
                     EHQSKIDWNNYLKQKKGLLKMPQTEGTQKALDITQEPNTGGERFNRNSSFLGEESSPR
                     TAPKPVVDEPQPDFPTNVPFHFSIDRSKKSTTKLRSSYEYASDKQLRQLNLFADDIRK
                     SANWYLDTVADSKVTYFYQDGDEITTLQVEFEKKNWMHLTGLAPVYSNWVPSLSESFI
                     EDIANGKGQFANLTLGRGVMEKIQILPILPEVLETDSFVFDDLSSVEKFQRLDANKAI
                     ITDNEQLILAFRFDDGNFYPATLLKPTKALNIQLNETNQEKIVLGVFIEKDGLVKTHK
                     VNEDYIKDGGNELKSIIENDELEPIQTAPLERNVQQEKIFPKDSDGDGLTDDEEIALG
                     TNPFSSDSDGDGTPDNVEKANGTDPTNASDNEVTRQQEVNKRDFTLSEMIKAKNTAAL
                     NQHLQDGIKQYFDSNTYKQYLEGMAHFNNYSPRNIQLIMSQFPEASMVASFQEWKKRN
                     GSVNKGEKAIYIQAPVAVIQKDKNRKPILNPETGEKETITYFKPVPVFDIKQVSPQEG
                     KELNLPKAMGTIPEQLDKDYYQNVYRSLRDISQNNNKVPIRFRELGQEDGFYSPQTNE
                     IVIKKGMSYERTLSTLIHEMAHSELHNKQSLTERFDGQLTRSTKELQAESIAYVVSNH
                     LGFDTSQESFPYLVSWSKEKDGLANLTAQLEIVQEEAKSLMERIDQQLSQYQTVTLNK
                     ETQQLTKQEMKKQAHPFYQSLAAAKTSRTQVTTQEKEASVKKDNRPTMP"
     gene            30853..31152
                     /locus_tag="VBC94_000487"
     CDS             30853..31152
                     /locus_tag="VBC94_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978782.1"
                     /translation="MLTEEMMTTTEMILMTYLFEIDEWLKDKKVIQQQQAQLTKEELV
                     TVIKNDLTTFQANQATDYEKKLQTTLTTLEALSEAQFQKMKLEILSWGPNVKHVD"
     gene            31495..32055
                     /locus_tag="VBC94_000488"
     CDS             31495..32055
                     /locus_tag="VBC94_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978783.1"
                     /translation="MITPSELTHRIEHTTLSEAIELFEDKVLRKSLNNYDDWYKRDVQ
                     KEYERINYDGAFFFFVEPDLGSSRGGVSDVIIEEQEKVALLLLLVEAYERYIDVNTGI
                     KDWLGYDCIFCDVVVSNETAAKRLTQMEYEAIKDLIVTVIDHYVPSMTVMETDEYKEF
                     KQGQTPNDTVIDNVQITLPLFNKREK"
     gene            32058..32432
                     /gene="mobC"
                     /locus_tag="VBC94_000489"
     CDS             32058..32432
                     /gene="mobC"
                     /locus_tag="VBC94_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEO6978784.1"
                     /translation="MVEKRRTRINRFELRTTDGEANKLRRRITLSGKKTFQAYALKML
                     LEGKIETYDYSELRQLRIEVNRIGQNINQLVRYVNTFEEFDQELFTALQEEVKELQQM
                     IVQEFKTKEVAKQHGSDQSLSD"
     gene            32404..34044
                     /locus_tag="VBC94_000490"
     CDS             32404..34044
                     /locus_tag="VBC94_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEO6978785.1"
                     /translation="MVVTKVFQIKQIKNLKRAIDYITRDDATLIKVEQHDEDDAFDYT
                     LTADGEVHKKLISGHHLIDPGDRDAIYDDFILLKQSVDELYDNATLSDLKNDNRVLAH
                     HIVQSFSPDDKLTPEEVNEIGRKTALEFTGGDYQFVVATHVDKGFLHNHIIFNTTNEV
                     TLKKFRWQKNTARNLFQISNKHAELYGAKILEPKLRTSYTDYSAWRRQNNFSYEIKQR
                     LNFLLKHSISIEDFQQKAKALDLHIDFSGKFAKYRLLVPLDGKLQEKNTRDRTLSKKR
                     IYSFEQIQKRVAMNEVVYTVDHIKEKYEEEEAKKAEDFEMKVRIEPWQISEITRQSIH
                     LPVTFGLEQKGTVSIPARLLDQNEDGSFTAYFKKKDYFYFINPDKSTDNRFIQGSTLV
                     KQLSLNSGEVILTKNWEMTELERLVKEFNFLSANKVTNSKQFQEMQERFLEQLEKTDQ
                     TLAQLDSKVAYLNKVLGALSDYQDGLVPSEVTMAILEKAKVAKNTDRNLIRKEIKELQ
                     IEREALQEARDRIVKDYDFSHKLTQEYSPKESRKKSII"
     gene            34181..34588
                     /locus_tag="VBC94_000491"
     CDS             34181..34588
                     /locus_tag="VBC94_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978786.1"
                     /translation="MGKSYYYVEVETIQGESLIFQLPNDLQGAMRDYRHENPETWEAL
                     LKEALINIPSASYTKANHYQPIIRLAKVKKSFSLKKQQRRRSRGQFLTKDNWQQKGFK
                     HFRDSLRFIQHDYPFKIRVRLLLDFWRWKRRSY"
     gene            34619..35902
                     /locus_tag="VBC94_000492"
     CDS             34619..35902
                     /locus_tag="VBC94_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
                     /protein_id="HEO6978787.1"
                     /translation="MTRFDEKIFVIEMAKLREKEFLKRIRDYDEQIATTMRQHGYKRV
                     DSSERTVLFTFGEITFARNRWRKGRKTRYPVDEWLGLKPYMRYSPELMLHMAEHASKL
                     SYREVCRTIETAYGLLVTKDAVLKAVKLAERLLIEKEHYRFLQHVEHPQKIQAERIYL
                     EGDGVMVKTTSGGDERHNTDLAHFLIHTGSRKSGKNRYSLTNKHEIINANYDKAREEL
                     LDYLYNHFEITDKTILITNSDNGKGYTKRVFQEVKKALGIKHHEHFWDSYHLNKQLKD
                     FFKPYPYVLQDLAFKAIQTHKKSLLETVFDTVETLIETEEDYINYYAFRQKLFRNFRD
                     TRPSQLRGLTSRGIGAMESQHRKVTYRMKHRGMYWSVTGACTMAKIILLERINKLGDL
                     FFGDWRKQYKRYQGRGLGAGHLVNHYPHDAVLIRR"
BASE COUNT        12664 a         6484 c         7419 g        10077 t
ORIGIN      
        1 attgctgaga ttgttgagca agtaggaatt ggttttgatg atatagaatt gtatggtaaa
       61 tacaaagcaa aattatcttt tgataagatt gaagctgtaa gatctcaaaa agtaggaaag
      121 ttaattttag taactgctat taatccgaca ccagcaggtg agggaaaatc cactatgagc
      181 attggattag ctgatgcttt aaataaaatt ggtaaaaaaa cgatgattgc gcttcgcgaa
      241 ccttctttag gtcctgtaat gggtattaaa ggaggagctg ctggaggtgg atacgcacaa
      301 gtactaccaa tggaagatat taatttgcat tttactggtg atatgcacgc aatcacaact
      361 gctaataatg ctttatcagc tctacttgat aatcatattc atcaaggcaa tgaattagat
      421 attgaccaac gtcgagtgat ttggaagcgt gtagttgatc ttaatgatcg tgctcttcgt
      481 caggtgatcg ttggcttagg aagccctgtc aatgggatac cacgtgaaga tggctttgat
      541 attactgttg cttctgaaat aatggctatt ctatgtttag ctactgacct atcagattta
      601 aagaaacgcc tgtcaaatat tgttgtggct tactcaaggg accgtaaacc aatctatgtt
      661 aaagatttga agattgaagg agctctcaca cttatcctaa aagatgctat caaacctaat
      721 ttggttcaaa ccatttatgg gacaccggct cttgtccatg ggggaccatt tgctaatatt
      781 gcacatggat gtaattctgt cttagcgact tcaacagctc tacgcttagc agattacgtt
      841 gttactgaag ctggttttgg agcagacttg ggcgctgaaa agtttcttga tataaaaacg
      901 ccaaaccttc cgacttctcc cgatgcgatt gttattgttg ctacattgcg tgcattgaaa
      961 atgcatggag gtgtttcaaa agaagatctc tcacaagaga acgtcgaagc agttaaaaga
     1021 ggttttacaa atctcgagcg tcacgttaac aatatgcgtc aatatggtgt tccggtagtt
     1081 gtagctatca atcagtttac tgcagataca gaaagtgaaa ttgcaactct taaaacctta
     1141 tgtagtaata ttgatgtggc agttgaatta gcaagtgtgt gggaagatgg agcagacggt
     1201 ggccttgaac ttgcacagac tgttgctaat gtgattgaaa cacaatcatc caattacaaa
     1261 cgcttatata atgatgaaga cactattgaa gaaaaaatta aaaaaattgt tactaaaata
     1321 tatggtggta ataaagttca ttttggacct aaggcacaaa tacaattaaa agagtttagt
     1381 gacaatggct gggacaagat gcctatttgt atggcaaaga cacaatatag cttttctgat
     1441 aatccaaatt tatttggtgc tccaactgac tttgatataa ctgttcgtga atttgttcca
     1501 aaaacaggag ctggttttat agttgctctt actggagacg tattgacaat gcctggttta
     1561 cctaaaaaac ctgcagctct caatatggat gttttagaag acggtacagc cgttggatta
     1621 ttttaaataa taaagaggta gggcttgtga tgctagcctc tttttattgg ggattagatt
     1681 ttaactagag ataatttaga ataatagaat gctattcaag tgacagtatg ctataataaa
     1741 atgattgatt tggagggggg aaaaacttat cagtaataaa gtcaaaattg ttcgtttact
     1801 taataagagt aagaaaagtt tactaagggg tatatttagt cgaacaaccg ttatcgctat
     1861 tttattgatt ttacaattat tgtttttact agcctcttac tcatggctag agcagtatcg
     1921 tgtttggtta gcaaccgttg agcatatctt aacaattgga gcggtcttat atttagttaa
     1981 tagtgaaatg gatgctttgt cccgagtaac ttggttaata ttggtaatga ttgccccttt
     2041 actaggggcg atgttcctaa tgtacaccaa attcgactgg ggttatcgtg gtttgaaaca
     2101 aagattggag acattgattg atgagagtca gatttatcta gaggatgatc ctgaaacttt
     2161 gaatcagctt aagtcgagta catccacaac atatcatttg gttcaatatt ttgaaaaagc
     2221 acatggaaat ttcccagttt atcgaaatac tgatgtgaca tttctaccaa cgggagaagc
     2281 attctttgaa aaaatgaaag aagaactact aaaagctaag aagtatatct ttttagaatt
     2341 ctttatcatt gacgaaggaa taatgtgggg agaaatactt agtattttag aacaaaaggt
     2401 ggaagaaggt gttgaagtcc gtatcttata tgatggtatg atagaaatta caaaattatc
     2461 ctttgattat acgaaacgac ttgagaaaat aggaataaaa gcaaaagcat tttctccgat
     2521 ttctcctttt atttcaactt attataacta tcgtgaccat cgaaaaattg ttgttattga
     2581 tggagtagta ggcatgactg gtggtgtaaa cttagccgat gagtatatta atcacattga
     2641 gttatttggg cattggaaag attcaggtat tatgttaaag gggaaagcag tggatagttt
     2701 cctcctactt ttcttacaga tgtggtccat tactgaggaa aaaatgttag tagcgcctta
     2761 cctcggtgta cacgatgatt tagttgaaaa tgaaggctat gttattcctt atggagattc
     2821 tcctttagac actgataaag taggggaaaa tgtttatata gatattctta atcatgctag
     2881 agaatatgtt tacatcatga caccttattt aatattagac agtgaattgg aacatgccat
     2941 tcagtttgct gcggaacgtg gtgtagatgt acgtattatt atgccgggta tcccagataa
     3001 gccaatccct tatgctcttg ctaaaactta ctatcaagca ttaataaaat caggtgttaa
     3061 aatatatgaa tacacccctg gatttgttca ttcaaaaata ttccttagtg ataatacaaa
     3121 ggctgttgta ggtacgataa atcttgatta ccgtagcctg tatcaccatt ttgaatgtgc
     3181 tgtttatctt tataaggtag atgcaatcca agacatttat cgagattata tggatacgtt
     3241 aaataaatca aggttagtta gcttgaaaga tataaataat attccaaaat tccaaaaagt
     3301 aattggcata gtgacaaaaa ctattgctcc cctgctttag aaataattta tttttaattt
     3361 aatattaaaa gtaaactgaa gaatctagtt atatttaaaa agtaagtgtc tagagtttcg
     3421 ggaaaaacaa aattaaatat ccttatagct taagagaacg agaaaatcgt tctctttttt
     3481 cgttggtcaa aactagtgaa aggatatacg gcaatgtcag ataatattga aatcatcatg
     3541 gatattgaaa ctctagacat atttgaagtg atagatcttg atgaagagaa agaagtgact
     3601 gtttcagatt ctgaaacttt tcctaaagaa gagaaagcat ccccatatga tgatctaaac
     3661 agaatttggt taagaaatgg ttattattac aaaccttcaa gccttctatg ggaagttgta
     3721 agagatggca tcatccatga gttaagccct cgaaataaac acggaacata tccctgatgt
     3781 gaacattcca gaaatggcac ttggggtcat gtcaagcaac tcacacaaga aggagcttta
     3841 tggcttactt tggaactgaa tccagacttg atgaattatg tgtaggagat tcattgtgaa
     3901 gagtagagac gatgagattc tttatactgc acggctatat ctatttggtg tgattccatt
     3961 gtggaaagta aagcacacaa agcaagagtg gaagagatgt atacttttgc aacccagtaa
     4021 caagcttatt gaacttcagt ttattgaagc tgaagaggag gaaaaggagt gacaaatgag
     4081 cccattgatt atttttaaca ttagttttgc ttttgtcttc taccccatgt ttatcagtaa
     4141 ctatcacaag agagagcctt atttattgaa cttattactc tttgtcataa atgctttggc
     4201 atcaatgtat acgattttta attacttggg cttgctaaag tgagtagttg gaaaaggtga
     4261 gatgttatga cacaagaaga aatagacaac atgattgaag actgggtcag gcatggtttg
     4321 ccagatgacc tgaagcgttt aataccagcg gaagaacaag agaatggaaa cagggattaa
     4381 gaaaggtcac ttgaaggaat ccgtagagaa aagaattcat tcgcaacata tgaggataga
     4441 acccaatatt tctaaccgaa gcacaaggat acgtaacatt tcatatgatc aatctaaaaa
     4501 attgtatcga gtcacaattg tcagaaatgg tattcgatat ggtggtaagc actttaagaa
     4561 attaggtgaa gttcagaagt acaagaagca actgttagaa gacataaaaa gaaaggtaaa
     4621 ggaatccaaa tgagtcaaac ggcaatagca caactaacaa agaatatgat ggtattcaac
     4681 gtagttgtat tgagtcttgg ttatttcaat gtaattcctt ataaatggtc gttaatactt
     4741 tggggaagtg cagtatttac agcaatattc aatgaaattt caaaacaaat ccctgatgaa
     4801 aagtaagagg aattacttca gctatgattt tttcccttaa cgcaggaatt ttgattatcc
     4861 cttatattaa taatagctct agatattttt aagaaagaaa ggaaagtacc gaatgacaaa
     4921 acaagtgcaa cagtcacgtg agttagtatt gaaagaaatt atgcaatcac aaggtgtgac
     4981 aagaacaaaa gcttgttcca ttttgaaaga gctagaggaa tttggtttat ttcagttttc
     5041 tgatagcggt cagtttatgt tgaaagtggg tgcttgacat gaaacgtatt tctgcaaggc
     5101 aagttcaatc aagtgaacga ttttatagaa tacccaaaat atttgttgaa tcgccatttt
     5161 ataagtcaat gagtacagat gctaaattca cctatgctat tttgaaagac cgttttgaat
     5221 tatctcttaa aaataattgg atcgataaaa atggagatgt ctatctgatt tatacagtta
     5281 ctgaactcca agaaatttta ggatatggta ataaaaaagt tatcaagctg aaaaaagaac
     5341 ttcaagagta tggtctcttg gaagaggttc gtcaaggttt gaataagcct aatttactat
     5401 acctgggaaa tgttgactat caaaatgtcc ctaccgaaga aattcactcc aaaccatcat
     5461 cagtagtgga agtgtcaaaa caacacttcc agaaaaatca aggaaatgtc aaaatgacac
     5521 gtcaaaaaat gtcaaaacga cacgctaatg atactgagaa tagtgagact gaattatcta
     5581 acgatactaa tcgataccag gacgaaattc cattatccac tctttcagat tctgaagctt
     5641 tccaaagagg ccaacatggt tttctgtctg accaaactat tcagagactc tctttgtttg
     5701 gtaaggatgc caaggtcctc gaaaataaaa tctatcaagc aaagcgtcag gttgaaaaag
     5761 actatagtca tctcttgaat catcaagaaa tgatttatgg tgaagtctgg tcgctggaac
     5821 tggaacgtga agtagataag cttatcttca aaatcaagac aggtgaacat gaaggtaagg
     5881 caatcaaaaa tatctcaggc tatttctaca agatgatggt gcgcttttgg aagatggctt
     5941 tactgattga aaaagaacaa ggtttcatag ctttatccaa ccaaagtgac tatgccaagt
     6001 cattccctga agaatgtccg agtctaatca gctattatta tccagataaa ctgtcagaaa
     6061 gcaaactgaa tcagtattta tcagagctgt cagaaaggat tgaaacatgt tagacaagcc
     6121 atctaggaat agtcctcgag ataagctcaa aaatcatctt aaggcctgtc aaactcctct
     6181 taaaaattat cccctaaagg cagtttttct ggcagttatc tttatagcta tcgtcttttc
     6241 tttcttttct caaatactaa tatccctaaa ggctatgggc ttcactacct tatcgttact
     6301 gatactgtta gtcttgggtt atccatgtca tcttcaaagt ttatatcatg atttaaaact
     6361 tagtatctat caatatcagc aggataaaat tgattttttc aaaacgtatg gtgaaaagca
     6421 agaagacgtg attgatgaca tacgaataat ctatgaaact gataatacgg taacggtaca
     6481 gtttatctat caggggcagc cctcgcaatt atccttgtct aagggtgcta ttccccaatc
     6541 atacgctaat caacaattag tagttatagc aagatgtcga gcgattgcta aagagcactt
     6601 atcagcgtat ctcaccctgt ttgaaataag agatttagct caagagtatc agacaatact
     6661 caaacacccg ataataacag aaggtttact ttcagacaat gatattcttc aattgagtga
     6721 agcgcctgat aaaccgatgg tatcgtttca gctagcacta atcacgcaac caaaagaaag
     6781 tgagacaagc aaatgattat caaagaacga aatggatttt tgaccattcc caaatctatc
     6841 tttagtttac caccagagga aaaaactcaa gctcttaaag agcttgagga agacatggaa
     6901 cagcacaaag cgaaaggggg tgactccact tgacaactat cactcaaaat agagtaaagg
     6961 agctacgtaa acaggctggc ctttcccaac aagcattagc ggatcaaatt ggcgttatgc
     7021 gtaaaacgat ctcgcattgg gaattaggga accataggat atcaacggat aatgctaaaa
     7081 agctctcaca gttctttcag gtatcgatag atgacctgct agaataaaat attctgatgt
     7141 gggaaaagac aaagattaat ctgaaaagtt aattttgtcg tcacgcctct agtacgaacc
     7201 taaagtaaaa ataaacaacc taaaaaggaa ttgaccgaaa tgtcgttaaa ctaaatagat
     7261 cattgacaat tcaatacaag gcttgtcctg atgcagcgtg gctatggata aaagaaaaat
     7321 gggggcgagt agcaagctag aagagaatat ctagtccagc cacagtaacg aaaaagcatg
     7381 attttatgaa atcataagct aaaggagtag agggtcctag actataaccc cagcacatga
     7441 aataccgaga taggtttacg aacctatcca gcacagcgta aatcgctttt caacttcaag
     7501 gagaaatcta tgacagatga aacaaaaaaa gtccacctaa gggacttacg aaacttagga
     7561 aagcaaggtg gtgctacagc acgcttagag gatgggacag aacttttcct aaagccaggc
     7621 tatgctgtaa gaaccgctaa gggctatgtg gatggtatac ctagagatgt agagttccat
     7681 ttgacttata gaagcatctt taatcaaatt cgtaccatca agaaagacgg tcacctagta
     7741 gctagaaaag agcgaagtcc atatggactt gtacttcaat tgacaggaaa aggctataag
     7801 agaccataaa aggagattag acgaaatgaa tcagataaaa attatcacag gacttacagt
     7861 cgcaacacta tccgcagtgg tagggaatgt atacgcagaa gatatcacac cgacagcacc
     7921 agttaatgaa ccacaagtat caagcgaaac cgcaaaaacg cctcaagtta cagaaagtca
     7981 agttaacagc gcaaaagtta ctgttgatca agcaacaagt gatgttaata accaacaaat
     8041 tgtagttgat gaggctcaaa agcagaaaga tcaatcacaa caaaatcttg caaaagccac
     8101 atcaacagta actgaagctt ctacgattgc tgctgaggca acacctgaag ttgttaaaga
     8161 agctactaaa gctgtcactg aagcaaagga agctgttacg gatgctgaag ctaatgtagc
     8221 cgatgcaaaa aagacggaac aaaaagctaa tcaagaggta caatcacaag ctaagactgt
     8281 tgatggaaat gttaaagttg ttgctgataa agaatccgaa gtgaaacaag cagagggtgc
     8341 tgtaactaca gctcaagaag ctattgatag caaaaccgct aacactaatg catcagaagc
     8401 agaaaaagca gtgactgaga agcaaacaaa gcttgaaact gctgaaacaa atcttacaga
     8461 ggcacaaaaa caagatgcca aaatcgctga agaaaaacga cttgcagaac aagaagttgt
     8521 tagtaaacaa ttagctgtga cagatacaca aactcttttg aagaagcttg ttactgagat
     8581 taataacgag aaagtaagca ctagtttaga aaaccaagcc tattttaacc aacgtgacgg
     8641 tgcatgggca cgctattatg gcaattatac ttttgccgct actggctgtg taccaagtag
     8701 tttagcaatg gtatttacgg agttagctag acgccaaatt actccaactg aaatcgctaa
     8761 ttatctttgg aacaattcaa atgagtttaa taaaaactat ggtggaacaa gtgggaaagg
     8821 gttagtacaa gctactaaac atttcggttt tgtaccaact catttagcat ctcaatctgc
     8881 tattgtggaa gccttacagg caggacacca tgtcctagca gctgtccaac aagataaatt
     8941 ctctccttgg ggtatccagt acagccatga gattgtctta cgtggttact caaatggcaa
     9001 tacttatgtt tacgatcctt ataatcgagc taacattggt tggtaccctg ttgctaattt
     9061 atggaatgaa caaagtagag acgccattga tacatctggt gtaggcgtac catttttcaa
     9121 aattacaaca caaaaaatgg cccaattaga agctcagaca gcacaagttc aatcttctct
     9181 aaatacagct aaaaatcagt tagctaaaac acaagacgta ttaagagcac tagaggcaac
     9241 acctctcaaa acaccagaag cacaagttaa gcttaatcaa gccaaagaag ctctagctct
     9301 tgcacaagca gactatacta aagctcaaga agctgttaag ttagctagtc aagacttagc
     9361 tgttaaagaa gaaacactta aaaatgctca ggctgattta ttaacgaaac aaacagcctt
     9421 gaaagatgct caaactgttc tcgtggctag tcaagttaaa ttagctgatc ttaaagcaac
     9481 tttagctact gttgaaaata acgttaagaa agctcaagct actttaacag atgccaaagc
     9541 aattgttggt caaaaacaag caaaattact tgctttacaa aatgccccta aaattctagc
     9601 agatgctcaa gctaaacttg taactgctaa aaatgattta gctaataaaa tggctatttt
     9661 agatgaagca gtcgcaaaac taaaatcctt acaagccgtt caagctgaag cccaaaaaca
     9721 ataccatgtt gtttttgaag cctataaagc agtacgagat gccaaagaac aagctaaact
     9781 tgcagaaagc tataaccata ttatcgcagg aggcggtgag gctatcccag ttgttgacga
     9841 aacaggtaaa ataactggat atgtagatgg tagccagaaa gcagttgcta atgaagtgac
     9901 tcttgcctta actagtaaca gtcaagcccc ccttgaaagt ccagttaata aagaaaacca
     9961 aaacgtaaca aaatcatctc aagcattacc acatactggc gaagcaggct tatccctctt
    10021 atcaattgta ggggcaggga ttatctcaac tctaggcctt gttagcttga aaaaacgtcg
    10081 cacacattaa ttaatgaaag gaatttacta tgtcaaaaaa acaattatta ctcttaactt
    10141 gtgtttccac cattgcactt gcaggaacta ccgcatttgc ggaagacgtc gtcccagtag
    10201 acccagcagt accatctact gaagtggtca caccaccaaa cccagtagat cctagtttac
    10261 caactgatac aacagatcct agcaccccag tagcacctga gaaactacca gacaccccaa
    10321 aaccagcaga gccagaaaga ccaaaagaag agctaccagc gccagtgcct ccgacaactc
    10381 catcagctgg taaagaagac aaaccagaaa ctgttccagg tcaggctgaa acgcctaaag
    10441 aagaagtaaa acctgaaaac cctaagtcta gtgaagacaa gaaaaaagaa gaacttgtcc
    10501 cagataatgt ttcaaaagac attattgaaa aggtagatac cacgacaggt gaagtggtct
    10561 ttaaaccgat tccagttaca ccagacaaaa cagttatcgg tactaaggac agtcaggtga
    10621 ttattcgtga ccaagcaggc cgtacacaag tcgttacccc tgagagcctt ggtggccacg
    10681 ttaatcctaa tggtaccgtt acacttaaag acgctacagg tgctgaaaag acgctcccaa
    10741 agacaggtga taaacaaagc gtgcttggtt acttggggat gatgttaatt tcggttttag
    10801 gctacgctgt taagaaaaaa gtatcgcact aatcacatga cctagaatgt cactaaactt
    10861 aacttagaaa atggagaaaa caatatgaat tcacaagaaa caaaaggtca cggatttttt
    10921 agaaaatcaa aagcatatgg tttagtatgt gggattgcac ttgccctagc agctgcattt
    10981 acattagcta ctagtcaagt gtctgctgat caagttacaa ctcaagcaac aactcaaaca
    11041 gtaacgcaaa atcaagcaga aacagtaaca tcaactcaac ttgataaagc agtagataca
    11101 gctaaaaaag cagctgtagc tgttacaacc acaacagcag ttaatcatgc gacaactact
    11161 gatgcacaag ctgatttagc taatcaaaca caagctgtta aagatgttac tgcaaaagca
    11221 caagctaata cacaagctat taaagatgct actgctgaaa atgcaaaaat tgacgcagaa
    11281 aataaagcag aagcagaacg tgttgcaaaa gctaataaag caggtcaagc agaagtagat
    11341 gctcgtaaca aagcaggtca agcagccgtt gatgcacgta ataaagctaa acaacaagcg
    11401 caagacgatc aaaaagcaaa aattgacgca gaaaacaaag cagagtctca acgtgtaagt
    11461 cagttaaatg cacaaactaa agcaaaaatt gatgctgaaa acaaagatgc acaagctaaa
    11521 gcagatgcga ctaatgcaca attacaaaaa gattatcaag ctaaattagc ggaaatcaaa
    11581 tcagttgaag cttataatgc aggtgtacgt caacgtaata aagatgcaca agctaaagca
    11641 gatgcgacta acgcacagtt acaaaaagac tatcaagcta agttagcact ttataatcaa
    11701 gctaaaaaag cgaaagaaga agcagataaa cagactatta ataatgttgc ttttgatatc
    11761 aaagctcaag ccagaggcgt tgataataaa gagtatggaa actcaatcat gactgcaaaa
    11821 actaaacctg acggaagttt cgagtttaac catgatatga tcgatggtgt gaagacaatc
    11881 ggctatggga aattgacagg taaagttaat catcattatg ttgctaacaa ggatggctct
    11941 gtgacagcat ttgttgactc tgtcactctt tacaagtacg agtatcgtaa tgttgctcaa
    12001 aatgctgctg ttaatcaaaa tattgtattt agagttttaa caaaagatgg tcgtcctatt
    12061 tttgaaaagg ctcataatgg taacaaaact tttgcagaaa ctttaaacaa aactttacaa
    12121 ctcaatctta aatatgagct taaaccacat gcttccagcg gtaacgtcga agtcttcaaa
    12181 attcatgacg actgggtaca tgatactcat gggtcagctt tagtgtctta cgttaataat
    12241 aatgatgttg ttcctaacgt tgttatacca aaacagccaa caccaccgtc aacagaaaaa
    12301 gtgactcctg aagcagaaaa accagtacct gaaaaaccag ttgagcctaa atttgtaaca
    12361 cctacattaa aaacttacac tccagtcaag tttattccaa aggaatacaa accagtacca
    12421 atcacaccag aaaaatttac accagaaaaa tttaatccag ctcaaccaaa agtgaaacca
    12481 catgtggcta ttcctgaaaa gattaactac tcagttagtg ttcatcctgt tttagttcca
    12541 gctgctaatc cttcaaaagc tgtcattgat gaagcaggtc aatctgttaa tggtaaaacc
    12601 gtattaccaa atgcaacatt agactatgtt gctaaacaaa gttttagtca atacaaagac
    12661 attaaagctt ctgcagaagc tatcgcaaaa ggttttgcat ttgtagatca accaaatgaa
    12721 gcgttagctg aattgactgt taagtctatc aaagcatcta atggtgatga tgtatcaagc
    12781 ttgttagaaa tgcgtcatgt tttatcaaaa gatactttag accaaaaact tcaatctctt
    12841 attaaagaag caggaattag tccagttggt gagttttaca tgtggactgc aaaagacccg
    12901 caagcttttt acaaagctta tgtgcaaaaa ggtctagaca ttacttataa cttatcattt
    12961 aaagttaaaa aagagtttac taaaggtcaa atcaaaaatg gtgttgcaca gattgatttt
    13021 gggaatggat atacaggtaa tattgtagtc aatgatttga caaccccaga agtccataaa
    13081 gacgtgttag acaaacaaga tggcaagtct attaataatg ggactgtaaa acttggtgac
    13141 gaagtgacct acaaacttga aggatgggtt gtaccagcaa accgtggtta cgatcttttt
    13201 gaatacaaat ttgtggatca gttacaacag acacatgatc tttacttaaa agataaagta
    13261 gttgctaaag tagcaattac acttaaagat ggtacagtca ttaaaaaagg gactaattta
    13321 ggagagtaca cagaaacagt ttacaacaaa acgacaggtc gttatgagct tgcctttaaa
    13381 aaagagtttt tagctaaggt ttctcgtgaa tcagaatttg gtgcagatga ttttattgta
    13441 gttaaacgta ttaaggcagg tgatgtttac aatactgctg atttatatgt taatggatat
    13501 aaagttaagt cagaaacagt tgtgactcat actcctgaga atccaagacc agttacgcca
    13561 caaaaagtaa ctccagctaa aggattacct caaactggtg aagcaagcgt tgcccctctt
    13621 acagcgattg gagcaattat cttatcagct ctaggcctcg caggctttaa aaagcgtcaa
    13681 aaataaccaa gttaatggat aagccaacaa agtagattac tttgggtcta tccattctct
    13741 ctagcaagta acccctcatt taattttcca tgattcctct gaaaatcaat cgggttgctt
    13801 gttagggtga gtggatagat cttggcttat cactagagta gtgaccgaaa agtcataaaa
    13861 ctaggcagag aggaaaccta aaaaagataa gacagaaggt ttcctcgttt gatcaagact
    13921 agaaccctct agtcttgatt tttttatgaa gaaagaaggt atcaaaatat ggaaacagct
    13981 gtccgtgttg taaccgctat gggtgcaata cttgcgattg tcggactcgg atgggtcttg
    14041 acaggcgctt ttgattattt tgcagggcgt aaaaacggaa gtcctcagat gatggaccag
    14101 gggtttactt ctatgatttc aggaggtgcc ttggcggtta tttctgctgg tgttgcggct
    14161 gcgattgtag ctgctatgcg agctatttca ttttaaaact agtttagaaa atgtggctaa
    14221 agaaacgaaa atgggtcatc tattgaccta aaatatggta taataaagat gataaagcaa
    14281 aacgagagga agggaagaag atgtctgaaa gctttgatta tgtggcattt gccagagact
    14341 ttgaaaagcg tcacggtcgt ccaccaacag ctgaagagtt agaaaaggct aatgtttcag
    14401 tggcgactgt cataacttca gataatcaaa agattgattt tcggatggtt gatagtttgg
    14461 attatgttga aggctataaa gataagtcta gctttggtga acgcttaaaa acagggctca
    14521 gctttgtgat acgaaatttc tttagagttc tcctcattgt tattcaaaca ccagtctatc
    14581 tcaccctctt cttctttaat ctgattaaat caacgattgg tgtttttgtt atttggtttg
    14641 tatcaaaatt tgtattagga tggttggtcg gtattattgc tggattaatt tatggttttg
    14701 acttatataa aaatccattt cctagtccaa tcaaagatat agttgatttc agttttggtg
    14761 ttaacttctt tgaggatgct gttcccaatt tcttcccaca tcctgtcgct gatgcctgga
    14821 ttattggcat taccatcgtt ttcttagctt tagtcatgac tttttcaaaa tcagaaactt
    14881 aatacttaag aacctttcga ggttcttttt atttttgaca gaaaggagaa tgatgaaaga
    14941 tatactttca gaactaacca agggactagg aagctataat tcctcagtaa ataattccat
    15001 gactgccatc aataaatccc tcatggccat tggatatatt ttagtatcca ttttattttt
    15061 acttgagctg ttatcttggt atcgctttat tcgtaaccaa ggtggggaga tgacttggaa
    15121 gttattttta gaagtggctg ttaaatattt cattgcttat ttcctagttt ctcaatcagg
    15181 ggcgattctc aatgccattt tatggtttac gaacggtgtg accaaactga ttggagtaga
    15241 cttgagtggt gacaacatgt ttgagtttgt atggttaaaa aaagggaatc taggtgtccg
    15301 tattctagta aatggactag ctgctatagc tggtgttgga gcaatgatct ctataaaagt
    15361 tatggtatta cttcgtttta ttgaactata tttctataaa gcaattgctc cagttatcgt
    15421 tgctttttgg atgggagatg atactaggca tattgccaaa aatttcctca aaacttttgg
    15481 ggcgattgct cttcaaagtg cagtaattgt tttattacta gctatatggc aaggactcaa
    15541 tatagatgct gtaattaaaa tatctaaaga ttcatgggta ggcgcttttg cagcaggatt
    15601 tagttacata ggtaagtgta ttgtctttct gatattgttg tttggttctc agcgcaaagc
    15661 caagcagtta ttacaggtgt cttgatagac caatcactat aatatatgga aggacattct
    15721 aatgaattta gaaaaactaa aacaaagacg agacgaactt atccgagata atgagtggat
    15781 ggacggtctc atcaaggaaa aagaagaaaa gctgtgggag ctgaaagtca gaagtatcac
    15841 tgcttcagaa ctcgctcgct ctgctatgca atcagctatg cgtactgctg gaattgttga
    15901 ttctttttat ggaccatctg ctcaatcatc agataatcaa gctaacaaag cagtagaagc
    15961 ggttcataat aagagctgtt tttttatgct agaaaggaaa agcgatagaa atgattaatc
    16021 aaggtcagga ataccaatat ttcaaagata aaattagtca tttagaaagg gaagtgagcc
    16081 gattgtctcc ttatgaatac gaacaccggc tattaaagga tgttatagcc gattgtctct
    16141 tacaaggaca gataacagta agtgaattgc cacaagctat tcgcttaata caaggcgatg
    16201 atctttttta cacctatgca tggcggtttg tggaagctac gggagattgc caagcaggta
    16261 tcaccatctt aaaaatactt caagatgatt tgaactattt ctttgcaatt gggaaactgt
    16321 ctcaaaaaca gtactcacag tggctggaga agtggctaag ttttttagaa cgtggtagga
    16381 tagcttttaa aggagagaaa gactttgaac gctattttca agaccaaaaa gaagctaatc
    16441 gaagcttatt cagtgatttc aacttatgat aggcagttaa gtaaaaagca gtttctttga
    16501 actgtttttt tcatgcaaga aaggagagct atgaacaaat taggaagtga attttttaag
    16561 gctatagatc aatttgagcg aggtattatc ggaagcttta cttggagaca aattttgatg
    16621 ttttttggaa ttgtcttggg agtcactctt gcaacggtga tttcattgct gcagcttcca
    16681 agtattctct tttacctctc actggcttta acagttgtcc cagcctttat ctatggcatc
    16741 aaaaaagatg aacacctcaa agaggtcatc acctttaggc tcaaggtaca agaaagggcc
    16801 taccaaaccg aatatgaaag tgaggaaaac tatggtgcgt ttattcccca aaagggtgtc
    16861 caggaatgga atgaccctaa cgaaagctga taaagaacgt gcaaaacaat tacagaaagc
    16921 cctaaaggct agtacccaaa atacgattca atacacgtcc ctctttgaag atgggctcat
    16981 gcacattgtc gatgaagcct attccaagac ctggcaatta ggtgatacga attacatcac
    17041 aggtagtgaa gacgaaaaac tagatattat tgaatactat gttgatgctt taaatggtct
    17101 agatagtgaa aatacctatc agttgacgat tcttaatcgt cctgtcccta caactttact
    17161 ggatcagatt acctatgact tagaaggtga tgagcgagat gtctttcgga aagaatacaa
    17221 taatattatc cgctctcgct ttgcgaaaga tcaaaataac tttaaagtgg aaaaatttgt
    17281 gacggtctcg acaagggcta aagatcggaa acaagcttac cgtaagttaa atgatgttga
    17341 cacccatttc acaacacagt ttcaattagt tgatgtcctt ctcaagtcac tagatggaac
    17401 agaaagactc aatatctttg cggatttact gagagggaac ccttatctcg atgtggacta
    17461 taaggatgtg agaatttcag ggcttagcac taagtccttc atcgctcctg gtcgcctttg
    17521 gtttcaacct gaccagtgta ggatggataa gaaatattgt aaaatcctct ttgccagaac
    17581 tttcccggcc tttcttaatg accgactcat taaatcctta accgatattg gcattgaatt
    17641 atctatttcc attcatgcta agccttatga cgtggctgaa gcagtgaaga aagtcaatac
    17701 agctgaagct ggtgttaaga tggacatggt taaatctcaa gtagccgcag ctcaaaaagg
    17761 catctcggga aacttagcag tttctagtgt ggcccaagat acggcagatg aagctgagaa
    17821 atggaaaaca gaaattaatg ataacgacca gaaaatgttt tctggcgttt ttttggtcat
    17881 ggtaaaagct gatagtgccg aagaattggc agactataca agtcgcatca aacaggctgg
    17941 acgcaaacac gtgattgagt ttgaggaaac ttactatcac caagaggaag cgctcaatac
    18001 tatgctgccg attggaaaga cttatataga tgtgaaacgt cgctttatgc gagatatgac
    18061 cactaccaat attgcgacac aaattccctt taccaacacg gacttacaat ctcaaagtcc
    18121 actagctaat tactatggtc aaaatcaaat ctctaataat atcatctctc ttgaccgaca
    18181 acgggattta tatacgtctt caggtgttat tctgggttct tcgggttctg gaaaatcagt
    18241 ttttgtgaag atcaatgaag tcattccagc tattctacgc tttccgaatg atcgggtcat
    18301 catagttgat cccgaggaag agtacgtcga tatcggccgt gcctttggcg ctcaagttat
    18361 tgatatctat ccaggaacaa aaacccactt taatctcatg gatattcctg acctagataa
    18421 acttcgagat gaagataaag attttgtagg gcaaaaagca tctcttatca tgggattgtt
    18481 tgaaaatatc cttcaagaag tgactgacga tgatgtctct ctgattgacc gtgtcactcg
    18541 cctgtgttat gaacaaatta ctgatcgcac cccaacccta aaggattggc atgatattct
    18601 cttggaacaa cctgaagagg aagcgcaaag tctcgcttta aaatcggaaa gctatactaa
    18661 aggatctcaa gatatttttg cttatgaaac caacgtagac ctcaataacc aagtcgttat
    18721 ctttaactta aaaaaattat caggtaagct aaaacccttt gccatgatgg tgattcagga
    18781 ttatatttgg caacacgtcg ttgatcacaa agatgagttt gtgactaggg tttactttga
    18841 tgagatgcag aatcaatttg aaacagatga ccaagctgct ttcttttcta aaatgtatgc
    18901 acgtatccgt aaatacgggt caatcccaac agggattacg cagaatgtgg aaaccttgct
    18961 ttctcggaaa gaagggcgta atttacttta taatagtgaa ttcctcgttc tcttgaaaca
    19021 aaagaagacg actattccct atttgcttca aaccattaac ttaaccgatg ctctcgttcg
    19081 ctatattgaa aagccaaaag cgattggaac gggcttaatt attgcaggta caacaactgt
    19141 cccatttgaa aatcctattc ctgaggatac agaactattc cgcttagttg caacggatgc
    19201 ttatcgaaaa atggaggaag aaaatgtttg aagatgtcac tttacaattg cctaaaaatg
    19261 aagaaaagaa gttgatggct cactattatc aattacatca ggaagccatt gaagaagctt
    19321 tagctggcaa agaattatgg aaaccctaca ttcgcatgtc tggactatct caatggctag
    19381 atgtttcaac gacaactatt gttaaatggc aaagggaagg catgccccat attgtcatcg
    19441 atggggtaac tttgtatgac aaacgaaaaa ttgcaacatg gttaaaccat tttgaaagaa
    19501 ataaaatcat ctctcaagaa aagcaaatga agattttccc aggctactta tcaatcaagc
    19561 atgtatttgg gacaattatt acctattcta agaaagtcgt tctagatcta atccaagatc
    19621 ttagagatga ggatattcaa gacttttctt attggtcccc actgtacttt ttagaaacgg
    19681 atgaactagg ccaacctgtt tatctaactt atttagtgaa tgaggtaggt ataccgctat
    19741 atccagactg ctattactac attacattaa caaaagaaca agaaagcaaa ttagaaagtc
    19801 tagaactatc aatttaacaa gaaaaaagga ggttagcacg atgagtgcag ttccaatcgc
    19861 tgcagcgtat ctagtacgaa aaagacaaga agagaaaaag gtacgccaaa agagtgagaa
    19921 ggagctaaaa aaaataaaag ttatggtaac tggtgaagag ccggtctatt atggacatgc
    19981 tttgaagtcg aaaatgaaaa cagaaaaata ctctcgctcg ccagttattg gagagtatgc
    20041 cctaaaatta tccaagaagc atccagggaa agtaacatgg atagatattg cggtgttatt
    20101 gggtctaata cttgcaggac tagctacgat ttggagcgtc tatgctgtta ttcatgatac
    20161 tttttaaagg agtgacatat taataaagaa aggaggcaac cgtgacagaa gaaaaacaag
    20221 atcttagaag tcgagttgta agaaaacgtc agattaaagc aagtgtccac agacataacc
    20281 agtcttccgc tagaaaggct caaaaagcag caaaaggtga atttcaaata gccactaaag
    20341 agttaaaaga agctagagaa gcctatcaaa aagcacaaac agactttagg gctggaataa
    20401 tagacgacga agctcttcaa aaagccaaga acagacttaa acaagctcaa aataagtata
    20461 aagatgctaa aaaggtcaaa aagaaagtac gcaaaaccaa tctaactctt agtcaaaaag
    20521 ccaggcagac gggtaggcgt atcaccaaac gaacaggtca agaaatccta gaaatggggt
    20581 tagaacaaga tgatacttta tctgatttgg tgaggactaa gcatcagatt cgtcaaaccc
    20641 gagtcatggc taaacaagca ggacgtgcaa ccggtaaaag tgtcaaattg acttatcgtg
    20701 gtgccaaggg aacggtaaaa ctaggagcta aaggaactaa gtacgcctat cgtcaaacac
    20761 gaaaaggaag cgtaaaagct actcaagcaa gtcttagagc agcttctaaa accgctcaaa
    20821 ctcttgtggc tacttcaaaa gctttagtga gcgccattac gcccattatt gctaatccga
    20881 taacctgggt tgtggctgga gttattagct tgcttttgtt aatcgttatc ctggtatctt
    20941 cggtcttttc gagtaatgtg gtccaacaaa atgaattcac tctcaatcaa tcctggctcc
    21001 atatctctca aatggatcgc aagaagtctt ctgataaggt ggattactat accgatattg
    21061 attccatttt actctatatg aattaccgct atggtggaga gtgggaacct gatgccaagt
    21121 gggatgatgg ttttggaggg aaagtcacag gtgccttagg tttcaatcac ttctcagatg
    21181 ccctagatga tatctggaaa gaggaaaaca gtgatatcac caacctaaaa accatggctg
    21241 aactttatac taatgggaag aaatggatca agttctcaaa agatgactta gaagagtata
    21301 aggagattct agatagccaa gtcgaaacag gaaaatattt agcctatcaa gaactttcta
    21361 accccttcta ttcatctaat gatgacaaag cagattcaga atacttgact gtgacaaaac
    21421 gctatggcta taccgataaa gataccattg atccgacgac aacacttcaa gcaaaagcca
    21481 gtcaaaagtt gtatgcggtt atggatggga aggtaactgt taccaaaaaa gacttaaagg
    21541 gtgaaaaaac gaaaaccact aatctcatta ttgaaggtaa ggaggcccgc tttatttatt
    21601 atgatatctc aaatagtcgg gtgaggacag gagatagggt tgaaacgggt caagaattag
    21661 ctacagtatc aggaagcacc caaaaaatag cgtatgctaa gcgctacgga gaagtagatg
    21721 ataaagatgc tacatggtta aaaaagatta gggatagaga ggtttcttac ggctatacga
    21781 tacaaacaaa gatgaataag aaagaagtct gggtacttac caacccaggc ttttattttc
    21841 cctttgtgaa gtatgcagaa acaactattg tgactcaaac tccatctgaa atgagtggtc
    21901 gagccaagca attctatgat ttggttaaaa aatatttgcc ccaggcaacc gataatggga
    21961 ttgcagctat tgtaggttgc tttggagtag agtctggcat caaccctaag cgagccgaag
    22021 gtgattatct cagtccacca gtaggcgctt ctgattcttc ttgggatgac gagtcctggt
    22081 taacgttagg tggtccagct atctacggcg gtggctatcc gaatattctt catcgaggtc
    22141 ttggtttagg acaatggaca gatacttctg acggtgcgaa tcgcaacacc ttattacgag
    22201 agtttgctaa aaaagaaggc caaaaatggt atagcctaga acttcaagta aagttcatgc
    22261 tagagggaga taatccctat tatatcaata cgtttaagag agttgcgact aataatgggg
    22321 atgtagatag tctaacagct gagttcttag ccaaatggga aggtgtacct ggcaataaat
    22381 tggctcaacg tcaagctatc gctaaacaag ctctaatgtg gtttaaacag ccaacacact
    22441 caggtggtgc aagcactctt caaagttctt ggaattttcc taaagaatat gcttctaaac
    22501 taaagagtta tccatcggct gctactgtat ctaggtcact aaatggcaat acttatccaa
    22561 ttggtcagtg tacctggtat gtttataacc gcctttcaga agctggtgct ccagctatta
    22621 actggttagg gaatggccaa gattgggtta gaaatttagt cgctaaaggg tggaccttta
    22681 gtaataaacc agtggcaggt gctgtttgtt caactgctgg tggttttgac tacaccatgc
    22741 ctacttatgg acacgtatcc tatgttgagc atgtcaatga agacggtacc tttttagtga
    22801 gtgaatgtaa tgtcatgggg attcaaaacc agattcattg ggcagtgaaa ttgaatcaaa
    22861 gctattacac ttttgcaata cctcctaaat aaatagaaac gaggcaaaat aaatgattga
    22921 tacttttaaa gtcaagtggt ttaaacgagg cttgagcctt cttgctctcc ttcttgtctt
    22981 ctttttagga cttggttttg gccagatggg aaactcaagc cactccaagg atagacctaa
    23041 acagacagcc actaagaaaa caaggacgaa tactattaca caaacacagg taaaagaatt
    23101 tttggttgct tactatacca aaaaagattt agaagaaaac cgtaaccgct ataaggacta
    23161 tatgacagaa ggtctctata atgcgacgat ctcagaagaa gataaagccc aaaataaggc
    23221 ctataaaggc tttgtagtta attttgagtt tcaagacgct aatatctata ttgaccaagt
    23281 ccataaacag gtcatctgtg aggtcaccta tactaatgac ttacttcaaa aaaagaataa
    23341 caaagacggt gctcaaatag gtgttgtcaa tcatacaacc atccagttag tttatactaa
    23401 gctgaatggg aaaaatttga ttaaccagat gagtacgatt ttgttaaccg attcagctaa
    23461 tccttctgaa gaaactgaaa gttatggagc aatcgcccca tcaaatacta aagattaaac
    23521 aataaaggag ttatatcatg ataaacgaag aacatatgaa tcaaaccagt tcatcaaaaa
    23581 gccgtcggca gaaatccact caagaacgtt tagaggataa gattaagcag ctagaaaagg
    23641 ctcaagaaag tcaagacgct cttcaaaaga aaatcaaaac gttaaaagat gatattgcca
    23701 tcttagaagg caaacggcaa caggaaatca tggccgaata tggtattgat ttacaaggtt
    23761 tagaagccat tttagccact cacaaagaaa aacttggagg tgaggggtaa tgtttcggat
    23821 tcagaatcaa tctgatggga aggagcagga atttcagaac ttggcagcac ttctatatac
    23881 attagaagga gaagaaaagc ggtgcctgca gttaaacatg agagctacat tttatctgtt
    23941 tcatctggat aaaaatgaag aagtcctgga atcgatggaa ttaaccattc cctctcaaga
    24001 agacaaagag gttcaggaac tcatcggaga ctttggctta aaaggagaaa caaagaaatt
    24061 cttttggcag cgctcgatgg acaagaagcc aatccccatc aaaacaagcc caaacaatca
    24121 tcaagagttg ccatctaaaa caggtgcctt aaccttgtta aaaaggctgg tgtggttgct
    24181 gccgatggta ttatcggtag tgagtatcta cttgtctttg gagaccttgc agttagttaa
    24241 gacacaatcg caggtgactc ccaaagtaga gacggtagtg accaatcaaa aggcagatgt
    24301 cttttgtcgc tatttcatta gcagttactt ctctcaaagt cctaatttag gggactattt
    24361 gtctgagaag ctttccatag atgatctgaa gactgataaa gtgacaccag tcagtgtctt
    24421 attagaaagt caggaaataa aaggggatac aactcttgtg acttatgtcg ttaatatcaa
    24481 agaggcacaa gatcatgtat caagtaagcg tctaacacta gctgttaggg ctgataagaa
    24541 ggctcagtat ggctatcttg ttatcaaaaa gctaagacta accgcttacc cctaatgaag
    24601 aaaggagaca aaacaaatgg atcaagaacg agttatggaa aaaggctcac gagttacctt
    24661 ggagacaggg aaacacttac ttcagttttt ggcttatgct gttagccaag gagctaagat
    24721 ttatacagag cataaacaat caggggagat gtcctggaag gatttcaatc agttaccccg
    24781 aacaaaagat catatcgact ttgaagaggc tgaggttaat cttgaaaaat tgagaaaaga
    24841 actcaaaaag tcaggggtta actttcactt taaaaagaat ggagatggca ccaagcaagt
    24901 ctggtttgaa gctattaatc aacaggtcat tcaagaagct ctcaggaaaa tcaatcagga
    24961 aattgtgtct aatcccaaag aagcactaga taaatacatg aagaaacctg atgaattaac
    25021 ccctaaacaa cagattgaaa aactcaagaa aacctctaag aaatcaacgg aagcagtaaa
    25081 aaccaagaag aaaggaaagg gcatctgatg gaactaaaac gtaaacgatt gcttccctat
    25141 cttctgctca gtttgcttct agcctacctg acacatcgtg cttatgtcct ttactgtatg
    25201 gcaccacagg cagatatgac caatcttttt gctccctata cctatgtttt tgaaaagata
    25261 acggaaccac cttacttcta ttggaatact agcccactag caattctttc tgctctagtg
    25321 ggcttttttg ttggcttact tttctatcta aagattagat tagatggtaa ttatcggcat
    25381 ggagaagaat cagggtctgc ttgctttgcg acggctcaag aactcaaagg atttcaagat
    25441 gaagaatcaa gaaataatat gattttctcg agagaagctc aaatgggact cttcaataag
    25501 cgcttgccgt ttgagcgaca actcaataaa aatgtcttag tagctggtct gcctggagat
    25561 ggaaaaacct acacctatgt aaagccaaac ctcatgcaaa tgaattcctc ttttatcgta
    25621 acggacccta aagggttgct tgtccgagaa gtaggaacaa tgttaaaaga aaatggctat
    25681 caaatcaaaa tctttgatct ggttaactta accaattcgg atatgtttaa cccctttagg
    25741 tatatgagat cagaattgga tattgatcgc attacagaag ccattgtcga agggacaaaa
    25801 aagggagatc gagaaggaga ggacttctgg aatcaggcca agcttctttt gaaccgtgct
    25861 ttacttggct atctctattt tgatagccaa gtacggcatt atgaacctaa cctttctatg
    25921 gtggcagatt tacttagaaa tttgaaacgc cctaatgaaa aggaactcag tgcagttgag
    25981 aagatgttta agcaattgga agaaaaatta ccaggtaact atgcctgtcg acagtgggaa
    26041 ctctttaaca gtaactttga ggcagaaacc aggactggtg tcttatctat tgtggccact
    26101 cagtactcca tttttgatca tgatgctgta acaaatctca ttaagactga caccatggaa
    26161 atggatactt ggaataccga aaagacagcg gtctttgtgg ccatttcaga aactaataag
    26221 gcttatagct tcttggcttc cacattcttt acagttgttt ttgaccaatt gactcatagt
    26281 gcagatgcta ttattcaagg ggaaaagaaa ggctatgaac tgaaagattt attacacgtg
    26341 caattctact ttgatgaatt tgccaattgt ggtaagattc cacactttaa cgaagtccta
    26401 gcctctattc gtagccgtga gatgtctata aaaatcatca ttcaagccat tagccaacta
    26461 gatagcctat atggcgttcc tgctagaaaa tccatggtca ataactgtgc gacgctttta
    26521 tttttaggaa ccaatgatga agatactatg aaatacttct ccatgcgagc aggaaaacaa
    26581 accattatac aaaccagcta ttcggaacaa cgtggacatc ggatatcagg aacaaccagt
    26641 agacaagcgc atcaacggga tctcatgacc ccagatgaaa tagctcgtat tggtgtcgat
    26701 gaggccttag tttttatctc caagcaaaat gtttttcgag ataagaaagc catggtatca
    26761 aatcatccta tgaaggatag actcgctaat cattatcaag ataagacctg gtatcactat
    26821 aaacgcttta tggaagaagg tgcggaattt attgatgcag tcatgacagg acaaattcca
    26881 gaagaccgca tttgggctcc tgatatgtct gactaccaag ccttcttaga agaaaacggc
    26941 tttgataaca cacgtcaatc agctcctgag acgtcagttc ataaccaagt ccctgtaact
    27001 aatcctgaaa tgccttcaga aagcccctct cagacaccag aaacctctac gaaactagtg
    27061 gatatggata ctggagaaat ttttgaactt ccccctgaag accgtgaagg tttcggagag
    27121 gtttcttttg atgaccaacg agtggaaatc tagtatgatg ttactcattt cgtattaata
    27181 gaaaggatta agatatgaca cctcaaattt tacgtcgatt agacgttaaa aaacagttta
    27241 ttgaagcaat agaccccttt gtccaccgtc aaaccctaaa gcccaaagcc gtgaattcat
    27301 ctaaaaccac aatgtcaatt caacgctata accatgcggg tactaaaatt cagttacgaa
    27361 tcggctatag taaggtactc atccgtatct tctccaatgg gaaaatcaat ctcacccatt
    27421 atgatctctt ttttgaccga gaagaaactc tggagataac tgatgccttt gacaatggtg
    27481 tttacacgca agatgaggta gatggcttta tcaaacaagc caaaaccttt atcaagcaag
    27541 cccttaaggg ggaagtctga tatggcgaat acatacgatt taatttcaca gcgtattgaa
    27601 gcacaacgac agaaactcat cgctatagat attgtggccg ttgcgagctc actaggactt
    27661 aacttgaaac aaggttcagg tggacatctc tattgggatg aacatgatag ttttcatatt
    27721 tacccccaaa ctaacacctt tcgttggtgg tcaagaagca tggggaccaa taccattgat
    27781 ttagtccaag tcatccaaga agagctaaca ggaaagaaac ctaactttcg agaaactgtc
    27841 aactttttag aaacagggca atttgaatcg gttacggtaa caccagtcgt tagagaaccc
    27901 tttaagcact acctcgctcc ttatgaacat cacaattttg acttagggcg acagtatctt
    27961 aaagaagaac gagggctatc tgatgagacc attgattttg ctttggcatc aggtagtatg
    28021 agctctgcga cattgaaaaa gggtgattat tttgaacctg ttattatctt taaaagtttt
    28081 gcggaagacg gccgaatgat cggcggtagc cttcagggga tcgttgaaaa taaagtccag
    28141 catcctgaac gtggccgtct caaacaaatc atgaagcatt cagacggtct agcaggtttt
    28201 catttggacg ttggaacacc taaacgtctc gtgttttcgg aagctcctat tgaccttctc
    28261 tcttattatg aattacataa ggagagttta cagaatgtcc gcttagtcgc tatggatggg
    28321 gtcaaaaaag gggtgattag tcgctatacg gctgatttat tgacagatgg tcagtattct
    28381 caaaccatgc ccagagagtc gattagagga gcgatagatg ccattaacca aacgaccaga
    28441 atccttaaaa acaatcccaa tctgattacc attgccgttg ataatgacga ggcaggccga
    28501 aactttatta aggagttaca ggaagatggc attccaatta ccatagacct cccaccacgg
    28561 aaagaacacc agagtaaaat agattggaat aattatctga aacaaaagaa aggattacta
    28621 aaaatgccac aaacagaagg cacacaaaaa gctcttgata ttactcaaga gcctaacact
    28681 gggggcgaac gttttaatcg caattccagt tttttaggag aagaatcttc tccaaggaca
    28741 gcaccgaagc ctgttgtgga cgaaccgcaa cctgattttc ctaccaatgt tccttttcat
    28801 tttagcattg ataggtctaa aaagtcaacc acaaaactgc gtagcagcta tgagtatgcc
    28861 tcagacaaac aattacggca gctcaatcta tttgcggatg atatcagaaa atcagctaat
    28921 tggtatttag atactgtcgc agatagtaaa gtaacttatt tttatcaaga tggagatgaa
    28981 attactactt tacaggtgga atttgagaag aaaaattgga tgcatttaac gggacttgct
    29041 cctgtttatt ctaattgggt tccttccctg tctgaaagtt ttatcgaaga tattgcaaat
    29101 gggaaagggc agtttgcgaa cttgacatta ggacgtgggg taatggagaa aatacaaatt
    29161 ttaccaatac ttcctgaagt gttagagaca gattcttttg tttttgatga cttatccagt
    29221 gttgaaaaat tccaacgctt agatgcaaac aaagcaatta taacagacaa tgaacagttg
    29281 atattggctt tccggtttga tgacgggaat ttttatccag ctacattatt gaagccaacg
    29341 aaagcactaa atattcagct gaatgaaacg aatcaagaaa aaatcgttct aggtgtcttt
    29401 attgagaaag acggattagt taaaacccat aaggtaaatg aagactatat caaagatggt
    29461 gggaatgagt tgaagtcaat tattgaaaat gatgaactag aacccattca aacagcccct
    29521 ttggagcgaa atgtacaaca ggaaaaaata tttccaaagg attctgacgg tgatggctta
    29581 accgatgatg aagaaatcgc tctaggcacc aatcccttta gttctgatag tgatggtgat
    29641 gggactccag ataatgttga aaaggcaaat ggaacagacc caaccaacgc ttctgataat
    29701 gaggtgacaa ggcaacaaga agtcaataaa cgtgatttca ctttgtcaga aatgattaaa
    29761 gccaaaaata ccgcagcgct gaatcaacac ttacaagacg gcataaaaca gtattttgat
    29821 agtaatacct ataagcaata cctggaaggg atggctcact tcaataacta ctcgccacga
    29881 aatattcaat tgattatgtc acaatttcca gaagcatcga tggttgcgtc tttccaagaa
    29941 tggaagaaac gtaatggctc tgtcaataaa ggtgaaaaag caatctacat tcaagcacct
    30001 gtagctgtta ttcaaaaaga taagaatagg aaacctattc ttaatcccga aacaggagaa
    30061 aaggagacca tcacttactt taaacctgtc cctgtctttg acattaaaca ggtttctccc
    30121 caagaaggaa aagaactcaa tctccctaaa gctatgggaa ctattccaga acaattggat
    30181 aaagactact atcaaaatgt ctatcgtagc ttaagagata tttctcagaa taacaataag
    30241 gttccgatcc gctttaggga gcttggacaa gaagatgggt tctatagtcc acaaacaaat
    30301 gaaattgtta ttaagaaagg aatgtcttat gaacgaacgt tatcaacgct gattcatgaa
    30361 atggctcatt cggaattaca caataaacaa agtttgacgg aacggtttga tggccagtta
    30421 acaagaagta caaaagagct tcaagcagaa tccattgctt acgttgtgtc gaaccatcta
    30481 ggatttgata ccagtcaaga gtcatttcct tatttagtgt cctggtcaaa agaaaaagac
    30541 gggcttgcca atttaacagc acaattggaa atcgtgcaag aagaagccaa aagcttaatg
    30601 gaacggattg atcaacagtt aagtcaatat caaacagtaa cactgaataa agaaacccaa
    30661 caattgacta aacaagaaat gaaaaagcag gctcatccct tttaccaaag tttagcagcc
    30721 gctaaaacat caagaacaca agtcaccact caggaaaagg aagcaagcgt aaaaaaagac
    30781 aatcgaccta cgatgccgta gtgcaacgta ggtcatcaca gaaagcaatg atattatcag
    30841 gaggacaaag atatgttaac cgaagagatg atgacgacaa ctgaaatgat tctaatgacc
    30901 tacttatttg agatagacga gtggctcaaa gacaagaaag tcatccagca acaacaggca
    30961 caactgacaa aagaggaact cgtaacggtc attaaaaacg atttgacaac gtttcaagct
    31021 aaccaagcca cagattatga gaagaaactt cagacaacac tgacaactct agaagcattg
    31081 tctgaagccc aatttcaaaa aatgaaatta gagatattgt cttgggggcc aaacgtaaaa
    31141 cacgtcgatt gacacaacat aagaagcgga caatgtaatg attgtccgct ttttactgtg
    31201 tgcatttttc aagactgaca ctcttaaaag ttgtgccatt gatcaaaatg taagatcgga
    31261 aaaaagtgca acatgacaaa gtgcacgtcg tgccaaaaag gggattgggg atttccccaa
    31321 aaagtaaaaa ggcaacagtc taattgtggg atttccgcaa ataggcttgt cgcccttttt
    31381 attttagcat gtgtccggac acgtgctaat cttgccagag aacaagccta ctggactctg
    31441 ggaaagctgt caagccctta gtaagggtga caatatagaa atgaggtatt gaatatgata
    31501 acaccaagtg aattaacaca ccgcattgaa cacacaacat tatcagaagc gattgagctt
    31561 tttgaagata aggtgcttag aaagtctcta aataactacg acgactggta taaacgagac
    31621 gttcaaaagg aatatgaacg gatcaattat gatggtgcat tcttcttttt tgtcgaaccg
    31681 gatttaggtt ctagtcgagg tggcgtatct gatgtcatca tagaagaaca agaaaaagta
    31741 gccttattgt tattattggt tgaggcttat gagcgttata ttgatgtcaa cacagggatc
    31801 aaagattggt taggttatga ttgtatcttt tgtgacgttg ttgtatccaa tgagacggcc
    31861 gctaaacgct taacacaaat ggagtatgag gcaataaaag atttaatcgt aacggtcatt
    31921 gatcactatg tcccaagtat gacggtgatg gaaacagatg aatacaaaga atttaaacaa
    31981 ggacaaacgc caaatgatac tgttattgat aatgtccaaa ttactttacc gctatttaat
    32041 aaacgggaga agtgaagatg gtagagaaga gaagaacacg aataaataga tttgagttac
    32101 gaacaactga tggtgaagct aataagttac gtcgtcggat taccttatca ggaaagaaaa
    32161 cctttcaagc ttacgcttta aagatgttac ttgagggtaa aattgaaacc tatgattatt
    32221 ccgaattgcg tcaactacga attgaggtca atcgtattgg tcaaaatatc aaccagctgg
    32281 ttcgttatgt gaacaccttt gaggaatttg accaggagct atttacagct ttacaagaag
    32341 aagtcaaaga actccaacag atgattgttc aagaatttaa aacaaaggag gttgccaaac
    32401 aacatggtag tgaccaaagt ctttcagatt aaacagatta aaaacttaaa acgagctatt
    32461 gattatatca cacgtgatga tgcgacgctt atcaaagttg agcaacatga tgaagatgat
    32521 gcttttgatt atacgctaac ggcagatggt gaggttcaca agaaattgat ttcaggccac
    32581 cacctcattg acccaggaga ccgtgatgcc atttatgatg atttcatttt attgaaacag
    32641 tctgtcgatg aattgtacga taatgccacc ttgtccgatt tgaaaaatga taatcgggtc
    32701 ttggcgcatc acatcgttca atccttttca ccagatgaca agctaacacc tgaagaagtt
    32761 aacgagattg gccggaagac agctttagaa tttactggag gcgattacca atttgtcgta
    32821 gcgacacatg ttgataaggg gtttttacat aatcacatta tttttaacac aaccaatgag
    32881 gtaacactga aaaagtttcg ttggcaaaaa aatacagccc gcaacttatt tcaaatttca
    32941 aataagcatg cggagttata cggtgctaaa attctagaac ccaaattaag aacatcctat
    33001 acagactatt cggcttggcg acgtcaaaac aattttagtt acgagattaa acagcgttta
    33061 aattttctct taaagcactc catttcaata gaggattttc aacagaaagc gaaagccctt
    33121 gatcttcaca ttgacttttc tgggaagttt gccaagtatc gcttactggt tcctttagat
    33181 ggtaagttgc aggaaaagaa tacacgagat cgcaccttat caaagaaaag gatttactcc
    33241 tttgaacaaa tccaaaagcg tgttgcgatg aatgaagtgg tttacacggt agaccatatc
    33301 aaagaaaagt atgaggagga agaagctaaa aaggcagaag attttgaaat gaaggtgaga
    33361 attgagcctt ggcaaatttc tgagatcacc agacagtcta tccatcttcc ggttactttt
    33421 ggcttagaac aaaaaggaac agtttcgatt ccagctcgac tgttagacca gaatgaagat
    33481 ggaagcttca cagcttattt taagaaaaag gactatttct attttatcaa tccagacaag
    33541 tcaacagata atcgcttcat ccaagggagt actttggtaa aacaattgtc tctgaatagt
    33601 ggggaagtga ttctcactaa aaattgggaa atgacagagt tagaaagact ggttaaagag
    33661 tttaactttt tgtcggccaa caaggtaact aattctaaac agtttcaaga gatgcaagaa
    33721 cgcttcttag aacaattaga aaaaacggat caaacactgg cgcagttaga tagtaaggtg
    33781 gcttatttaa ataaagtgtt aggagcttta tcagattacc aagatggctt ggtcccatct
    33841 gaagtcacga tggcaattct agaaaaagct aaagtggcta aaaatacgga tcgcaatctt
    33901 atccgaaagg aaatcaaaga attacaaata gagcgtgagg cacttcaaga agccagagat
    33961 cgtattgtaa aagactatga ctttagccac aaactcactc aagaatacag ccctaaagag
    34021 agtcgaaaga aatcgattat atagtatact aaaacaaaag aaaaggagaa gtctatctga
    34081 aaaggtgaga aacctgttga cgttttttca aaaataggta aatcattaac attgtatcga
    34141 caacttgcca tgctataata acaccaagga gatattgact atgggaaaga gctattacta
    34201 tgtggaggtg gaaaccatac aaggagagtc acttattttt caattgccaa atgatttaca
    34261 aggggctatg agagattatc gtcatgagaa tccggagact tgggaagcat tactgaaaga
    34321 agctttgatc aatattccaa gcgcttctta taccaaagct aatcattatc agccaatcat
    34381 tcgtttggcc aaggttaaaa aaagtttctc tcttaagaaa caacagcgta gacggagccg
    34441 tgggcaattc ctaaccaaag ataattggca acaaaagggc ttcaagcatt tcagggatag
    34501 tcttcgcttt attcaacatg attatccttt taaaattaga gtgaggttat tgcttgattt
    34561 ttggcgttgg aagcgacgct catattaaaa gaattagtat ttctagtata ataatcctat
    34621 gactagattt gatgagaaaa tttttgtgat tgaaatggct aaactacgtg agaaggagtt
    34681 tttgaaacgg attagggatt atgacgaaca aatagccacg acaatgcgcc aacacggcta
    34741 caaacgagtg gatagtagcg agagaacagt tctctttact tttggagaaa tcacttttgc
    34801 aagaaatcgt tggcgaaaag gaagaaagac ccgctaccca gtagatgagt ggttaggact
    34861 aaaaccctat atgcgctact ctcctgaatt gatgcttcat atggcggaac acgcttctaa
    34921 attatcttat cgggaagttt gtcgtacaat tgaaacagct tatggcctat tagtgacaaa
    34981 agatgctgtc ttaaaagctg tcaaactagc agaacggctc ttaatagaga aagaacacta
    35041 tcgtttctta cagcacgtag aacatccaca aaaaatccaa gcagaaagaa tctacttgga
    35101 aggggatggt gtcatggtca aaacaacctc tggaggagat gagcgtcata atacagattt
    35161 ggctcatttt ttaatccata caggatcaag aaagagtggt aaaaatcgct atagtctaac
    35221 aaataagcat gaaattatta atgctaacta tgataaagca agagaggagc tattagacta
    35281 tctctacaat cactttgaaa taacggataa gaccatcctc attacgaatt cagataacgg
    35341 taaaggctat accaaacgtg tctttcaaga ggttaagaaa gcacttggta ttaagcatca
    35401 tgaacatttt tgggacagct atcatctcaa taagcagttg aaagattttt tcaagcccta
    35461 tccttatgtc ctacaggatt tagcttttaa agcaattcaa actcataaaa agtcattatt
    35521 agaaactgtc tttgatacag tagaaacact gatagaaact gaggaagact atattaacta
    35581 ttatgctttt aggcagaaac tctttagaaa ttttagggat actaggccat cacaattacg
    35641 agggctaacc tctcgtggta taggtgccat ggaaagccaa caccgcaaag tgacttaccg
    35701 gatgaaacat cgtggtatgt attggtcagt aacaggtgct tgtaccatgg ctaaaattat
    35761 cttgttggaa cggataaata aactaggtga cctcttcttt ggcgactggc gaaagcagta
    35821 caagaggtat caaggaagag gtttaggtgc tggtcatttg gttaaccact atcctcatga
    35881 tgcagtgctt atcaggcgat gactacaaaa aaatctatcc tataacaacc gctaaaaaat
    35941 ttaactttag aggctttttt agtgtgttta tgccccgaaa cccttgcatt actgggttgt
    36001 ttgtggtaca ataatagtta gataaaatga aaaaaggtgc agaagcgcaa acttctacac
    36061 caccgattaa gacaaaaaag gactttgctt aatcagtcaa acagtattgt atcatgttat
    36121 aaacaatatc gtcaagactg aagcagagaa aacctttgtt tcagtctttt ttggaataaa
    36181 agaacctaga gttttcttag gttataaggg ttagttggac tttcccgaaa attcagacac
    36241 atacatttaa aaagtaaagg ttgcatttta actaaattat gttaaactac tgttatgcga
    36301 tgagtcgata tgtggtttta ccactattgc gcagggagat tataaacgca ggagcggatc
    36361 ttgataagtt gtgtgaacct tcttatcaca cttgaaaagg tgcccttagc ttactactac
    36421 ttgtaattca ttacaaactg tggtaagtag ctgaaaagca aaaaagaaag aaccagtttg
    36481 gttctttctt ttttttgcat aaataagtca caatttcttt ctaaaaatta tgtttttact
    36541 taactttaat tgaatatgct accatcacat tctttgtaaa atttttaaat aatctagttt
    36601 ctgatggttt agatgaagta ttaaaaatat actattatct catt
//
LOCUS       DATROQ010000011        36304 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.011, whole genome shotgun sequence.
ACCESSION   DATROQ010000011 DATROQ010000000
VERSION     DATROQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36304
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.011"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(63..1088)
                     /locus_tag="VBC94_000493"
     CDS             complement(63..1088)
                     /locus_tag="VBC94_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEO6978788.1"
                     /translation="MLHFENDYNKGAHPELLNALIETNDQGLSGYGTDSYCQQAADKI
                     REVCSCPQAEVEFLVGGTQTNQVVISSMLASYEGVIAAETGHVSSHEAGAIEFSGHKV
                     LTLPSHNGKLLASEVATYIETFYGDGNYQHMVFPGMVYISHPTEYGTLYSKAELEELS
                     KICKHYQIPLFIDGARLGYGLAAKDTDVDFPTIAALSDVFYIGGTKMGALAGEAVVFT
                     KKNRPKQFTTIVKQHGALLAKGRLLGLAFDRFFTDNLYLKIGKHAIDLAEELKIILEE
                     KGYSFYLKSPTNQQFIIVENTKLADLAKNVAYSFWEKYDDHHTVIRLATSWSTSREDV
                     TALRNVL"
     gene            complement(1208..2218)
                     /gene="tsaD"
                     /locus_tag="VBC94_000494"
     CDS             complement(1208..2218)
                     /gene="tsaD"
                     /locus_tag="VBC94_000494"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEO6978789.1"
                     /translation="MKDRYILAVESSCDETSVAILKNDKELLANIIASQVESHKRFGG
                     VVPEVASRHHVEVVTTCFEDALQEAGIVASDLDAVAVTYGPGLVGALLVGMAAAKAFA
                     WANKLPLIPINHMAGHLMAARDVKELQYPLLALLVSGGHTELVYVSEPGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMIKEDHLEFSFSGLKSA
                     FINLHHNAEQKGEALVLEDLCASFQAAVLDILLAKTQKALLKYPVKTLVVAGGVAANQ
                     GLRERLATDISPDIDVVIPPLRLCGDNAGMIALAAAIEFEKENFASLKLNAKPSLAFE
                     SL"
     gene            complement(2294..2701)
                     /gene="rimI"
                     /locus_tag="VBC94_000495"
     CDS             complement(2294..2701)
                     /gene="rimI"
                     /locus_tag="VBC94_000495"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEO6978790.1"
                     /translation="MESSEQALAIWSVLSDVYDKSPWSLSQISEDLKKDSTDYFFVYN
                     DGEVIGFLALQQLVGEVEITNIAVKKNYQGKGYAYQLMSMIADIEVPVFLEVRYSNIV
                     AQKLYERCGFVVLRKRKNYYHDPIEDAIVMRKG"
     gene            complement(2703..3395)
                     /gene="tsaB"
                     /locus_tag="VBC94_000496"
     CDS             complement(2703..3395)
                     /gene="tsaB"
                     /locus_tag="VBC94_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEO6978791.1"
                     /translation="MMKVLAFDTSSKALSVAVLNNMECLATVTINIKKNHSINLMPAI
                     DFLMQSIDLEPQDLDRIVVAEGPGSYTGLRVAVATAKMLAYTLKIDLVGVSSLYALTN
                     GFSENDLLVPLIDARRNNVYVGFYQNGDTVKPDCHTSLEEVLQEVGNKANVHFVGEVA
                     AFFDQIKKALPHAKITETLPCAVAIGRKGQKMKSVNVDAFVPRYLKRVEAEENWLKNH
                     CETNTEEYIKRV"
     gene            3574..3804
                     /locus_tag="VBC94_000497"
     CDS             3574..3804
                     /locus_tag="VBC94_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEO6978792.1"
                     /translation="MIYKVFYQETKERNPRREQTKTLYVTIDAANELEGRIAARKLVE
                     ENTAYNIEFIELLSDKHLEYEKETGVFELTEF"
     gene            3858..5537
                     /locus_tag="VBC94_000498"
     CDS             3858..5537
                     /locus_tag="VBC94_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO6978793.1"
                     /translation="MSNINLKPEEVGVYAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVENIDRIKALVITHGHEDHIGGIPFLLKQANLPIYAGPLA
                     LALIKGKLEEHGLLRDATLYEIHANTELTFKNLSVTFFRTTHSIPEPLGIVIHTPQGK
                     VICTGDFKFDFTPVGEPADLHRMAALGEDGVLCLLSDSTNAEVPTFTNSEKIVGQSIM
                     KIIEGIEGRIIFASFASNIFRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKINNIHTSGHGGQQEQKLMLRLIKPKYFMPVH
                     GEYRMQKVHAGLAVDTGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRHDLSEDGVVLAVATVDFDSKMILAGPDILSRGFIYMRESGDLIRESQH
                     ILFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDK"
     gene            complement(5695..6204)
                     /locus_tag="VBC94_000499"
     CDS             complement(5695..6204)
                     /locus_tag="VBC94_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6978794.1"
                     /translation="MEENMNIKQLKSKTMLGTVALVSAFSFASTNADANTYNYAVDVD
                     YLASAEEIAQAHPASNTFPLGQCTWGVKEMATWAGNWWGNGGDWAASAASAGYTVGTQ
                     PRVGSIVCWTDGSYGHVAYVTAVDPVTNKIQVLESNYAGHQWIDNYRGWFDPQNTVTP
                     GVVSYIYPN"
     gene            complement(6352..7698)
                     /gene="glnA"
                     /locus_tag="VBC94_000500"
     CDS             complement(6352..7698)
                     /gene="glnA"
                     /locus_tag="VBC94_000500"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEO6978795.1"
                     /translation="MTITAEDIRREVKEKNVTFLRLMFTDILGVMKNVEIPATDEQLD
                     KVLSNKAMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     EPFAGDPRGNLKRNMKRMQEMGYKSFNLGPEPEFFLFKMDENGNPTLDVNDKGGYFDL
                     APTDLADNTRREIVNVLTQMGFEVEASHHEVAVGQHEIDFKYDDVLKACDNIQLFKLV
                     VKTIARKHGLYATFMAKPKFGINGSGMHCNMSLFDNEGNNAFFDPEDPRGMQLSEDAY
                     YFLGGLMKHAYNYTAIINPTVNSYKRLVPGYEAPVYIAWAGRNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALSVLLGSGLEGIENKIEAPEPIETNIYAMTVEERRQAGIVDL
                     PSTLHNALKALEEDEVVKAALGTHIYTNFLDAKRIEWASYATYVSQWEIDNYLDLY"
     gene            complement(7732..8103)
                     /locus_tag="VBC94_000501"
     CDS             complement(7732..8103)
                     /locus_tag="VBC94_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6978796.1"
                     /translation="MKERELRRTMAVFPIGAVMKLTDLTARQIRYYEDQGLITPERTE
                     GNRRMFSLNDMDRLLEIKDFISDGLHISDIKNEYMQRQHKSKEKQKSLSDAEVRRLLQ
                     DELRNQGRFSSPSQHIGNMHL"
     gene            complement(8183..8722)
                     /locus_tag="VBC94_000502"
     CDS             complement(8183..8722)
                     /locus_tag="VBC94_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEO6978797.1"
                     /translation="MKTLKKLLSNYKFDIKKFKFGMRTFKTGLSVFLVLLVFHLFGWN
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMINVACNNKSGIIGGTAALLIITLSIPSGETILYVFARIFETFCGVFIA
                     MMVNTDIEILRKKLKNNKL"
     gene            complement(8985..10181)
                     /locus_tag="VBC94_000503"
     CDS             complement(8985..10181)
                     /locus_tag="VBC94_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEO6978798.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGAKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            complement(10316..11185)
                     /locus_tag="VBC94_000504"
     CDS             complement(10316..11185)
                     /locus_tag="VBC94_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEO6978799.1"
                     /translation="MKTTKVFSVLSLSVAVFMMTACQSNHSTKKADMSPDKVEQKTKF
                     KNISLSSNKLLAKENTMSVLWYQNSAEAKALYLQGYNVAKMKLDDWLQKPSEKPYSII
                     LDLDETVLDNSPYQAKNIKDGSSFTPESWDKWVQKKSAKAVAGAKEFLKYANEKGIKI
                     YYVSDRTDAQVDATKENLEKEGIPVQGKDHLLFLKKGMKSKESRRQAVQKDTNLIMLF
                     GDNLVDFADFSKSSSTDREQLLTKLQSEFGSKFIVFPNPMYGSWESAIYQGKHLDVQK
                     QLKERQKMLHSYD"
     gene            complement(11394..12404)
                     /gene="gap"
                     /locus_tag="VBC94_000505"
     CDS             complement(11394..12404)
                     /gene="gap"
                     /locus_tag="VBC94_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEO6978800.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGQFVKVSAEREPANIDWATDGVEIVLEATGFFAS
                     KEKAEQHIHENGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALQDNFGVKQGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVATLEKDVTVEEVNAAMKAAANDSYGYTED
                     PIVSSDIVGISYGSLFDATQTKVQTVDGNQLVKVVSWYDNEMSYTSQLVRTLEYFAKI
                     AK"
     gene            complement(12608..14686)
                     /gene="fusA"
                     /locus_tag="VBC94_000506"
     CDS             complement(12608..14686)
                     /gene="fusA"
                     /locus_tag="VBC94_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEO6978801.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQSLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYVDQANEYREKLVEAV
                     ADTDEDLMMKYLEGEEITNEELMAAIRKATINVEFYPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDEEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     SKVILESIEVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEARGNTQVVRAFVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNSGE"
     gene            complement(14841..15311)
                     /gene="rpsG"
                     /locus_tag="VBC94_000507"
     CDS             complement(14841..15311)
                     /gene="rpsG"
                     /locus_tag="VBC94_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEO6978802.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFEAIKEATGTDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            complement(15333..15746)
                     /gene="rpsL"
                     /locus_tag="VBC94_000508"
     CDS             complement(15333..15746)
                     /gene="rpsL"
                     /locus_tag="VBC94_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEO6978803.1"
                     /translation="MPTINQLVRKPRKSKVEKSDSPALNIGYNSHRKVHTKLSAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            complement(15965..16777)
                     /gene="purR"
                     /locus_tag="VBC94_000509"
     CDS             complement(15965..16777)
                     /gene="purR"
                     /locus_tag="VBC94_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEO6978804.1"
                     /translation="MKLRRSERMVVISNYLINNPYTLTSLNTFASKYGAAKSSISEDI
                     AIIKKAFEQAQIGDIKTVTGASGGVIFTPTIAEAEAKEIVEELRQRLSENDRILPGGY
                     IYLSDLLSTPKMLQSIGRIIANAYRGQKIDAVMTVATKGVPLANAVANVLDVPFVIVR
                     RDLKITEGSTVSVNYASGSSGRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTVSGMISL
                     LSEFDSTLVGVAVFAENAQEQREKMAYKSLLRVSEIDVKNNRVSVEAGNIFD"
     gene            complement(16874..17815)
                     /locus_tag="VBC94_000510"
     CDS             complement(16874..17815)
                     /locus_tag="VBC94_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEO6978805.1"
                     /translation="MKINQMKKDELFEGFYLIKKAEVRKTRAGKDFIAFTFQDDTGEI
                     SGNMWDAQTYNVEEFVAGKIVHMKGRREVYNGTPQVNQITLRNIKDGEPNDPRDFKEK
                     PPINVDNVREYMEQMLFKIENATWQRVVRALYRKYNKEFFTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPELNKSLMFAGIMLHDLAKVIELSGPDNTEYTIRGNLIGHIS
                     LIDEELTKILAELNIDDTKEEVTVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALNRVNEGEMTNRIFAMDNRSFYKPNIK"
     gene            complement(17805..19079)
                     /locus_tag="VBC94_000511"
     CDS             complement(17805..19079)
                     /locus_tag="VBC94_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEO6978806.1"
                     /translation="MLVIILIIVLASLTVTIISYPKMTELTKSVEKQLEDNADNLSDQ
                     LTYQIEVAQKDQILTLTNQLNRMQQEIYQLLTDMRTELNQHLTESRDRSDKRLELINS
                     NLSQSVQKMQDSNEKRLDQMRQTVEEKLEKTLQTRLQTSFETVSRQLESVNQGLGEMK
                     TVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTVSQYEREFPTVSGSSERVEYAI
                     KLPGNGQGDYIYLPIDSKFPLEDYYRLEDAYELGDKVQIELYRKSLLASIRKFAKDIN
                     NKYLNPPETTNFGIMFLPTEGLYSEVVRNATFFDSLRRDENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNANDISKILGNVKVEFGKFGGMLSKAQKQLNTASKSIDSLLTTRTNAI
                     IRVLNTVEEHQDQATTSLLNLPITEEEEINEN"
     gene            complement(19081..19713)
                     /locus_tag="VBC94_000512"
     CDS             complement(19081..19713)
                     /locus_tag="VBC94_000512"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEO6978807.1"
                     /translation="MTKIALFAGGDLTYFEYDFDYFVGIDRGSLFLLKNGLSLDMAVG
                     DFDSITEDELLYIKHYCSNIVSASAEKNDTDTELALKTIFKEFPEAQVTVFGAFGGRI
                     DHMMSNIFLPSDRDLEPFMSQIRLKDEQNIVTYLPSGKNQVSRIEGMSYVSFMPESES
                     TLQISGAKYELNKSNYFKKKMYSSNEFMTSPIEVELKDGYLIIIYSKDRG"
     gene            complement(19706..20368)
                     /locus_tag="VBC94_000513"
     CDS             complement(19706..20368)
                     /locus_tag="VBC94_000513"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEO6978808.1"
                     /translation="MSTNKIAPSILAADYANFANELKRIEETTAEYVHIDIMDGQFVP
                     NISFGADVVSSMRKHSKLVFDCHLMVVDPERYIEAFAQAGADIMTIHVEATKHIHGAL
                     QKIKEAGMKAGVVINPGTPVESLIPILDLVDQILIMTVNPGFGGQAFIPEMMSKVKTV
                     AAWRKEYGHHYDIEVDGGIDNTTIKAAAEAGANVFVAGSYLFKASDLPAQVETLRVAL
                     DD"
     gene            complement(20375..21247)
                     /gene="rsgA"
                     /locus_tag="VBC94_000514"
     CDS             complement(20375..21247)
                     /gene="rsgA"
                     /locus_tag="VBC94_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEO6978809.1"
                     /translation="MQGRIVKSLAGFYYVESDGVVYQTRARGNFRKKGQIPYVGDWVE
                     FSSQDQSEGYILSIEERKNSLVRPPIVNIDQAVVIMSAKEPDFNANLLDRFLVLLEYK
                     MIQPIIYISKLDLLDDLVVIDDIRELYQNIGYVFCYSQEELLPLLANKVTVFMGQTGV
                     GKSTLLNKIAPELKLETGEISGSLGRGRHTTRAVSFYNVHKGKIADTPGFSSLDYEVD
                     NAEDLNESFPELRRLSHFCKFRSCTHTHEPKCAVKEALTQGQLWQVRYDNYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            complement(21403..22254)
                     /locus_tag="VBC94_000515"
     CDS             complement(21403..22254)
                     /locus_tag="VBC94_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO6978810.1"
                     /translation="MLVDKKWRFEDSASYFACPKCQNPLIKESNSLKCSDNHCFDLSK
                     FGYVNLLGGKKVDEHYDKKSFENRQLVLENGYYNHILEAISKVLENNSQFHSVLDIGC
                     GEGFYSRQLVNKHEKTFLAFDISKDSIQLAAKSDQSRLVKWFVSDLANLPIQDSSIDI
                     ILDIFSPANYKEFRRVLSDDGILVKVVPVAEHVQELREKASQYLKQKDYSNQKILDHF
                     RENFEIISEQKVVQSYNCSQQERQAFIDMTPLLFSVDKTTIDWASISEITVGALIVIG
                     KKRSVSK"
     gene            complement(22258..23130)
                     /gene="rsmA"
                     /locus_tag="VBC94_000516"
     CDS             complement(22258..23130)
                     /gene="rsmA"
                     /locus_tag="VBC94_000516"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEO6978811.1"
                     /translation="MRIADKTVTRAILERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DKGVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLIPILADTLARFDNVQVVNQDIL
                     KADLQTQIQAFKNPDLPIKVVANLPYYITTPILMHLIESKIPFAEFVVMIQKEVADRI
                     SAMPNTKAYGSLSIAVQYYMTAKVSFIVPRTVFVPAPNVDSAILKMVCRDQPVVSVQD
                     EDFFFRVSKVAFVHRRKTLWNNLTSHFGKSEDTKAKLEKALEIAKIKPSIRGEALSIP
                     DFASLADALKEVGI"
     gene            complement(23210..23701)
                     /locus_tag="VBC94_000517"
     CDS             complement(23210..23701)
                     /locus_tag="VBC94_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978812.1"
                     /translation="MEYNVTEALTRAGVDLATLAVKGTATMVTSKIQSIKDVKDAATL
                     RATYDDLINELLAEREQAIRIAQSYKEEYEKINIDDEDIEYLSNTLEQGISLLYNFSG
                     LNEEQERTMKQLVTLLNKDTLKTMQLIGFNYKQAIGEPLTEACATAIKNKFITQNSKG
                     KRK"
     gene            complement(23720..24280)
                     /gene="rnmV"
                     /locus_tag="VBC94_000518"
     CDS             complement(23720..24280)
                     /gene="rnmV"
                     /locus_tag="VBC94_000518"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEO6978813.1"
                     /translation="MKKIDIQEVIVVEGKDDTANLRRFYNVDTYETRGSAIDEDDLER
                     IERLHNLRGVIVFTDPDYNGERIRKIIMNAIPTVRHAFLNRDEAKPGSKTKGRSLGVE
                     HASFEDLQKALSKVTQHFDDEDHFDITQADLIRWGFITASDSRKRREYLGNQLRIGYS
                     NGKQLLKRLRLFGVTKAEVEECMEGY"
     gene            complement(24267..25049)
                     /locus_tag="VBC94_000519"
     CDS             complement(24267..25049)
                     /locus_tag="VBC94_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEO6978814.1"
                     /translation="MIKIFDTHTHLNVENFEGKIDEEINLASELGVTKMNVVGFDQDT
                     ISKSLELSSQYAQVYSTIGWHPTEAGSYDDNIESMIISHLENPKVIALGEIGLDYYWM
                     EDPKDIQIEVFKRQIELSKEYNLPFVVHTRDALEDTYEVIKESGVGPFGGIMHSFSGS
                     LEMAQKFIDLGMMISFSGVVTFKKALDVQEAARELPLDKILVETDAPYLAPVPKRGRE
                     NKTAYTRYVVEKIAELRGITVEEVAEATYQNAVRIFRLDEKN"
     gene            complement(25231..25512)
                     /locus_tag="VBC94_000520"
     CDS             complement(25231..25512)
                     /locus_tag="VBC94_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978815.1"
                     /translation="MSLMWDVLTGKESVRTLKKQLDKELIHSVYAPRKRSKFHDIITS
                     HHKEIDEVAINTKTAIHKVANQLDEAIKGEFRSKVEEAIESNQQKYNHL"
     gene            complement(25522..25914)
                     /locus_tag="VBC94_000521"
     CDS             complement(25522..25914)
                     /locus_tag="VBC94_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978816.1"
                     /translation="MALIDIIEKQLADTQRKISDLDDAYHHSCCQFEEKLDDLSVRKN
                     KIINMLQETYDAVEYDLRYSNDSSDMMTLNRILGSYHDDLEQAYHKEYYALSAQEEEY
                     RANYIRQRSEHELTFEELQREKKRELMK"
     gene            complement(25905..27197)
                     /locus_tag="VBC94_000522"
     CDS             complement(25905..27197)
                     /locus_tag="VBC94_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6978817.1"
                     /translation="MEKVLTDLDRVNIAKQEYELGSQLDTLVKIMSQDKVLPIGKVAH
                     VQDGGKETGEQIYTITPNGTLDKPEDVKEVTVLFKGSTAPFGGDDWKTDWFKNDIPIA
                     SKLLLKKFGSQSVSHKQGTKQLEQSAHLLKEVMNKYPNAKISVYGHSLGSMNAQYAIA
                     ELDDNQIYRVQGAWIYNGPNLYSILTDKQKEQVDKIKGVVNNYVDSKDKIGIGYPKEG
                     SDNAVGIVKIVDSKAVSGGSWVANFTNQHIWGGYQYNPDGSLKLAEDKFAYKENYSVV
                     HTNVNQRMHAIKAKLSSFKSTGKEVSKNQAIFLDSQQAKVISSGLVTISKEAVEEVKK
                     VEQKAVAKAEELYQSLKVAPTGVTELSPQELEEAYAQSGVSYASIVGEAQTFFSNKTQ
                     AAESISHAYVELENQINKGVEAMLRQDSNLSGEFKQWH"
     gene            complement(27197..27589)
                     /locus_tag="VBC94_000523"
     CDS             complement(27197..27589)
                     /locus_tag="VBC94_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 domain-containing protein"
                     /protein_id="HEO6978818.1"
                     /translation="MKSIKLLSFIVIILLGLGFTVGGLKVTENNKQQELWEIANSKDA
                     KNAYQKWIYAEDEDAFKENAVIKSYDIDKESIKKNPMGGISVRLIINKDPNLYITCNL
                     DRDNQGHLVSQSSHQSPQLTHLLESRGH"
     gene            complement(27764..29026)
                     /gene="dltD"
                     /locus_tag="VBC94_000524"
     CDS             complement(27764..29026)
                     /gene="dltD"
                     /locus_tag="VBC94_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEO6978819.1"
                     /translation="MLKRLGKVFGPLVCALLLLVGLYFVFPVSQPHHLGKEKNSAVAL
                     TKAGFKSRVQKVRAFSDPKANFVPFFGSSEWLRFDAMHPSVLAEAYNRSYIPYLLGQK
                     GAASLTQYYGIQQIKGQIKNKKAIYVISPQWFVRKGANKGAFQNYFSNDQTIRFLQNQ
                     TGTTYDRYAARRLLKLYPEASMSDLIEKVADGQKLSNKDKQRLKFNDWVFEKTDAIFS
                     YLPLGKTYNQAIMPHVGKLPKAFSYNHLSRIASQDAKVATRSNQFGIDDRFYQTRIKK
                     HLKKLKGSQRHFNYTKSPEFNDLQLVLNEFSKQNTDVLFVIPPVNKKWTDYTGLDQKM
                     YQKSVEKIKHQLQSQGFNHIADLSRDGGKPYFMQDTIHLGWNGWLELDKHINPFLTEE
                     NSKPNYHINNKFLKKSWAKYTERPSDYK"
     gene            complement(29019..29258)
                     /gene="dltC"
                     /locus_tag="VBC94_000525"
     CDS             complement(29019..29258)
                     /gene="dltC"
                     /locus_tag="VBC94_000525"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEO6978820.1"
                     /translation="MDIKSEVLAIIDDLFMEDVSSMMDEDLFDAGVLDSMGTVELIVE
                     LESHFNIDIPIAEFGRNDWNTANKIVAGVTELCNA"
     gene            complement(29273..30538)
                     /gene="dltB"
                     /locus_tag="VBC94_000526"
     CDS             complement(29273..30538)
                     /gene="dltB"
                     /locus_tag="VBC94_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEO6978821.1"
                     /translation="MIQAFLEKLPHLDVYGNPQYFFYLILAVLPIYIGLFFKKRFALY
                     EIIFSLSFIVMMLTGSTFNQLKSLLAYVVGQSLLVFIYKAYRKRFNHTLVFYVTVCLS
                     IFPLFLVKLIPAISEDGHQSLFGFLGISYLTFRAVAMIIEMRDGVLKEFTLWEFLRFL
                     LFFPTFSSGPIDRFKRFNEDYINIPDRNELLDMLGQAIHYLMLGFLYKFILAYIFGSL
                     IMPPLKELALEQGGVFNWPTLGVMYAFGFDLFFDFAGYTMFALAISNLMGIKSPINFD
                     KPFKSRDLKEFWNRWHMSLSFWFRDFVFMRLVKLLVKNKVFKNRNVTSSVAYIINMLL
                     MGFWHGLTWYYIAYGLFHGIGLVINDAWVRKKKNINKERRLAKKPLLPENKWTYALGV
                     FITFNVVMFSFLIFSGFLDLLWFPQPHNK"
     gene            complement(30535..32070)
                     /gene="dltA"
                     /locus_tag="VBC94_000527"
     CDS             complement(30535..32070)
                     /gene="dltA"
                     /locus_tag="VBC94_000527"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEO6978822.1"
                     /translation="MIHDMIKTIEHFAETQADFPVYDILGEVHTYGQLKVDSDSLAAH
                     IDSLGLVEKSPVLVFGGQEYEMLATFVALTKSGHAYIPVDQHSALDRIQAIMTVAQPS
                     LIISIGEFPLEVDNVPILDVSQVSAIFEEKTPYEVTHSVKGDDNYYIIFTSGTTGLPK
                     GVQISHDNLLSFTNWMISDDEFSVPERPQMLAQPPYSFDLSVMYWAPTLAMGGTLFAL
                     PKTVVNDFKKLFATINELPIQVWTSTPSFADMALLSNDFNSETLPQLTHFYFDGEELT
                     VKTAQKLRQRFPKARIVNAYGPTEATVALSAVAITDEMLETCKRLPIGYTKDDSPTYV
                     IDEEGHKLPNGEQGEIIIAGPAVSKGYLNNPEKTAEAFFQFEGLPAYHTGDLGSMTDE
                     GLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSQYVKSAVAVPRYNKDHKVQNLLAYIV
                     LKEGVRDDFERDLDLTKAIKEDLKDIMMDYMMPSKFIYREDLPLTPNGKIDIKGLMSE
                     VNK"
     gene            complement(32083..32205)
                     /locus_tag="VBC94_000528"
     CDS             complement(32083..32205)
                     /locus_tag="VBC94_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEO6978823.1"
                     /translation="MKPYLSFIGRTLLYFGILLLLIYFFAYLGRGQGSFIYNEF"
     gene            complement(32216..33403)
                     /locus_tag="VBC94_000529"
     CDS             complement(32216..33403)
                     /locus_tag="VBC94_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6978824.1"
                     /translation="MFSDLRKKFVFLTMSILIVVVLFLFAVSNRYNQYWDEYDAYRIV
                     KLVAKNDYLGIPGDEPIALVTIDNQKMVKIQSNNTDLTNDVIEKSSLKLLEQGKKSRK
                     WKSFIYSIKEYKDKTYTIAIMDLASYEVPYARRFLILVFTIFGFCLLAAVSLYLSRFI
                     VGPVETEMTREKQFVSDASHELKTPIAAIRANVQVLEQQIPGNRYLDHVVSETKRMEF
                     LIEDLLNLSRLDEKRSKVNFKKLNLSVLCQEVLLTYESLAYEEEKCLNDTIEDDVWIV
                     GEESQIKQILIILLDNAIRHSLSKSAIQFSLKQARRKAILTISNPSAIYSKEVMDNLF
                     ERFYQAKDDHADSLSFGLGLSIAKAIVERHKGRIRAYQEKDQLRLEVQLPIDGFWTNT
                     MIN"
     gene            complement(33403..34077)
                     /locus_tag="VBC94_000530"
     CDS             complement(33403..34077)
                     /locus_tag="VBC94_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6978825.1"
                     /translation="MRLLVVEDEKSIAEAIQALLADKGYSVDLAFDGDDGLEYILTGL
                     YDLVLLDIMLPKRSGLSVLKRVREAGLETPIIFLTAKSQTYDKVNGLDLGADDYITKP
                     FEADELLARIRLRTRQSSLIRANQLRLGNIRLNTDSHELESKESSVKLSNKEFLLMEV
                     FMRNAKQIIPKNQLISKVWGPSDNSEYNQLEVFISFLRKKLRFLKADIEIITTKGFGY
                     SLEERT"
     gene            complement(34433..34567)
                     /gene="rpmH"
                     /locus_tag="VBC94_000531"
     CDS             complement(34433..34567)
                     /gene="rpmH"
                     /locus_tag="VBC94_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEO6978826.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            complement(34751..36106)
                     /locus_tag="VBC94_000532"
     CDS             complement(34751..36106)
                     /locus_tag="VBC94_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HEO6978827.1"
                     /translation="MKKESISIKDILWFIIPSLFGVLLLMTPFKYNGMTTVAVSVISK
                     TINQWINAVFPIHYIILLIIFISCVLALCYRLFRPSFIEKNDLLKEISDITIFWLIIR
                     LIGLALGLMTVLHIGPEMVCGKETGGLILFDLIGGLFTIFLAAGFILPFLTEFGLLEF
                     VGVFLTPIMRPFFQLPGRSAVNCVASFVGDGTIGIALTDKQYVEGYYTSREAATISTT
                     FSAVSITFCLVVLQNVGLTDYFGHFYLTVIISSVVCAFIIPYIPPLSRQSNSYYTKPK
                     KETSEVIPDGYNKIEWGLKLAIDKSKANGNMKTFVNNASKTVLSLWFGVMPTIMTVGT
                     IALIISVSTPIFKILGTPFLPFLELLGIPEADIASQTMIVGFSDMVVPSIMAAEIHSE
                     MTRFIVATVSIVQLIYMSETGAVILGSKIPINILELFIIFIERTIISLPIIVLMAHLF
                     F"
BASE COUNT        11299 a         7301 c         5800 g        11904 t
ORIGIN      
        1 ccaaaaacaa gactattatt ttgtaatagt cactactgaa taatatttct tttattaaaa
       61 actcacaaga catttcttaa agcagttaca tcttcgcgac tagttgacca actagttgct
      121 agacgtatta cagtatgatg gtcatcatat ttttcccaaa aactataagc tacattcttt
      181 gctaaatctg ctagttttgt attttctaca ataataaatt gttgattagt tggtgatttt
      241 aaatagaatg agtaaccttt ttcttctaaa atgattttta attcctctgc taagtcgatc
      301 gcatgctttc caatttttaa gtaaaggtta tccgtaaaga aacgatcaaa agcaagcccc
      361 agcaatcttc ccttggctaa taacgcacca tgctgcttta caattgtagt aaactgtttt
      421 ggacgatttt tctttgtgaa aacaactgct tccccagcaa gagcccccat tttggttcca
      481 ccaatataaa aaacatctga taaggctgca atcgttggga aatcaacatc tgtatctttc
      541 gcagccaaac cataccccag acgagcaccg tcaataaata aggggatttg ataatgttta
      601 caaatcttcg ataactcttc tagttctgct ttactataaa gtgttccata ttctgttgga
      661 tgtgatatat aaaccattcc tggaaaaacc atgtgttggt aattaccatc accataaaaa
      721 gtctcaatat aagttgcgac ttcgcttgcg agtaatttcc cattatgaga aggtaaagtc
      781 aataccttat gccctgaaaa ttctatggct cctgcttcat gactactcac atgacctgtt
      841 tctgcagcaa taactccttc ataagaagca agcatactac tgataactac ttgatttgtt
      901 tgagtaccac ccactaaaaa ctcaacttca gcttgtggac aggaacagac ttctcgaatt
      961 ttatctgccg cttgttgaca gtaactatct gtcccatatc ccgatagacc ttgatcatta
     1021 gtttcaataa gggcatttaa aagctctgga tgagctcctt tattataatc attttcaaaa
     1081 tgtaacattc cttaactcct ttattagtta aaaaaatagc gacaatatca tttgctaata
     1141 tttttgaata ttcaatcatt ataacaaata aaaaatatca aaacaagcgt tttgatatta
     1201 tgaacaccta taaactctca aaagctaaac taggtttggc attcaatttt aaagaagcaa
     1261 aattctcttt ttcaaactct atcgctgctg ctaatgcaat cattcctgca ttatccccac
     1321 ataatctaag aggaggaata accacatcaa tatcaggaga aatatcagta gccaagcgtt
     1381 cccgaagtcc ttgattagct gcaactccac cagcaacgac taaagttttc actggatact
     1441 ttaacaaagc tttttgagtt ttggccaata aaatatccaa aacagccgcc tgaaaggaag
     1501 cacataaatc ttcaagaacc aatgcttcac ccttttgttc tgcattatga tgtaaattga
     1561 taaatgcaga ttttaatcca gaaaaagaaa attcaagatg atcttctttg atcatcgctc
     1621 taggaaaatg gtaagtatct tgacccttat gagctaactg atcaatctcg cgacctgctg
     1681 gataagttaa gcccataaca cggcctactt tatcataagc ttctccaaca gcatcatccc
     1741 gagtttctcc tactattttg taatctcccg gttcagaaac atatactaat tctgtatgtc
     1801 ccccactgac aagcaaagct aacaatgggt attgaagttc cttaacgtca cgtgctgcca
     1861 ttaaatgacc tgccatgtgg tttataggaa ttagaggtaa tttatttgcc caagcgaaag
     1921 cttttgctgc agccatacct accaataagg ctcctactaa tcccggacca tatgttacag
     1981 caacagcatc caaatcgcta gcaacaatac ctgcttcttg aagagcatcc tcaaaacagg
     2041 ttgttactac ttcaacgtga tgacggcttg ccacttcagg aacaacacca ccaaaacgtt
     2101 tgtgactttc aacttgactt gcaataatat tagctagtaa ctctttatca ttttttaaaa
     2161 tagcaacact agtttcatca catgaacttt ccactgctaa aatatatcta tctttcataa
     2221 tgatattaat ccatcaatat cgtcttttcc attagtattg aggactattc atatcctttc
     2281 tatcatcaac tatttatccc ttgcgcatca ctatagcatc ttcaattgga tcatggtaat
     2341 aatttttgcg ctttcttaaa actacaaaac cacacctctc ataaagtttt tgtgctacga
     2401 tatttgaata acgaacctca agaaaaactg gcacctctat atcggcgatc attgacatta
     2461 actggtaagc ataaccttta ccttgataat tttttttaac agcaatatta gtaatttcta
     2521 cttcacctac caattgttga agagccaaaa aaccaatgac ttcaccgtca ttataaacaa
     2581 aaaaataatc tgttgaatct ttttttaaat cttctgaaat ctgcgataaa ctccacggag
     2641 atttgtcata gacatcagat agtacgctcc atatagccag tgcttgttct gatgattcca
     2701 tgtcaaactc tcttaatata ttcttctgta ttcgtttcac agtggttttt taaccaattt
     2761 tcctcagctt caacacgttt taagtatcgt ggaacaaacg catctacatt aacgcttttc
     2821 attttttgtc ctttgcgccc aattgctact gcacaaggta aagtttctgt aattttagca
     2881 tgtggtaagg ctttcttaat ctgatcaaaa aatgctgcaa cctctccgac aaaatgaaca
     2941 ttggctttat tccccacctc ttgtaagact tcttcaagag aagtgtgaca gtctggttta
     3001 acagtatcac cattttgata gaaaccaaca taaacattgt tacgtcgtgc atctataagt
     3061 ggtaccaata aatcattttc tgaaaatcca tttgttaaag cgtacaggct agatactcca
     3121 actaagtcaa tcttaagcgt ataagctagc atttttgctg tagcaacagc tacacgtaag
     3181 cccgtataag atcctggacc ctctgctact acgatacggt ccaaatcttg aggttctaaa
     3241 tcaattgatt gcattaaaaa atcaatggct ggcatcaaat taatgctatg attctttttg
     3301 atattgatag tgacagtcgc taaacattcc atattgttta gtacagccac tgatagtgct
     3361 ttgcttgaag tatcaaaggc taaaactttc atcatttaac ttcccttact tgattctacc
     3421 tcttatttta tgatataatc atgataattg caacgaatca aggttaattc tattactcta
     3481 taaaaactta ataatggttt gtcattgtct cacttaacgg cattgaccca tttttttata
     3541 gttagaaaaa ccgagagata agaaaggatt attatgattt ataaagtttt ctatcaagaa
     3601 acaaaagaac gcaacccacg tcgtgagcaa accaaaacac tttacgttac tattgatgca
     3661 gctaatgaat tagaaggtcg cattgctgca cgtaaactag ttgaagaaaa tacagcttac
     3721 aatatcgaat tcatcgagtt actttcagac aaacaccttg aatatgaaaa agaaactggt
     3781 gttttcgagc tcactgagtt ctagaaagga gttaaacata atagctagag taatctaccc
     3841 attatgataa acttattatg tcaaatatca acttaaaacc cgaagaagtt ggggtttatg
     3901 cgattggtgg tctcggtgaa atcggaaaaa atacttacgg tatcgaatat caagacgaaa
     3961 ttattattgt agacgctggt attaaattcc ctgaagatga tcttttggga atagattatg
     4021 ttatcccaga ctattcatat atcgttgaaa acattgaccg tatcaaggcc cttgttataa
     4081 ctcatgggca cgaggaccac atcggtggta ttccttttct attaaaacaa gctaatttac
     4141 cgatttatgc aggtcctctc gctcttgctc taatcaaagg taagttagaa gaacatggtt
     4201 tacttcgtga cgctacatta tacgaaatcc acgctaatac agagctgact tttaaaaact
     4261 taagcgttac atttttccgc actactcact ccattcctga accattaggt attgttattc
     4321 atacccctca aggtaaggtt atttgtacag gtgacttcaa atttgacttt acacctgttg
     4381 gggagcccgc tgacttacat cgtatggctg ctctaggtga agatggtgtc ctctgccttt
     4441 tatcagactc gacaaatgct gaagttccaa ccttcactaa ttccgaaaaa attgttgggc
     4501 aatctattat gaagattatc gaaggtatcg agggacgtat tattttcgcc tcttttgctt
     4561 caaacatctt ccgactccaa caggctgctg aagctgctgt taaaactggt cgtaaaattg
     4621 ctgtttttgg acggtcaatg gaaaaggcaa ttgttaacgg tattgaactt ggctatatta
     4681 aagttccaaa aggtaccttc attgagccaa gcgaacttaa aaatcttcat gctagtgaag
     4741 ttttaattat gtgtacaggt agtcaaggtg agtctatggc agcacttgct cgtatcgcta
     4801 atggtactca tcgtcaagtt actttgcagc ctggtgatac tgtgattttc tcatcaagcc
     4861 caatccctgg taatacaaca agtgtcaata aattaatcaa taccattcaa gaagctggtg
     4921 tcgatgttat tcatggtaaa attaacaata tccatacctc cggccatggt ggtcaacaag
     4981 aacaaaaatt aatgttacgc ttgattaaac caaagtattt catgcccgta cacggtgagt
     5041 atcgcatgca aaaagtccat gctggtttag cagttgacac aggtattcct aaagagaata
     5101 tcttcattat ggaaaacggt gatgttttag ctttaacgag cgactctgct cgtattgctg
     5161 gacatttcaa tgctcaagat atctacgttg atgggaatgg tattggcgat attggtgcgg
     5221 cagttctacg tgaccgccac gacttatctg aagatggtgt tgtccttgcc gttgctaccg
     5281 ttgatttcga ttcaaaaatg attttagctg gaccagacat ccttagccga ggctttatct
     5341 atatgagaga atctggggac cttattcgcg aaagccaaca tatcttattc aatgctattc
     5401 gtattgcact gaaaaataaa gatgcaagta tccaatcagt aaatggagct attgttaatg
     5461 ccttacgccc attcttatat gaaaaaactg agcgtgagcc tatcattatt cctatggtcc
     5521 ttacccctga taaataaaca aaagaacgga aaatactttc tccgttcttt ttaattgcct
     5581 atattagcaa aataagttaa tatatcaaat tacttctggg ataactatag aatataaaag
     5641 aactccaaac agtatcgtac ttttataccg tctatgtgag gagttcttta tagattaatt
     5701 cggataaatg tagctaacta cccctggtgt tactgtattt tgtggatcaa accaaccacg
     5761 ataattatca atccattgat gacctgcata attagattct aaaacctgaa ttttattagt
     5821 gacaggatct acagctgtaa cataagccac atgtccataa ctaccatcag tccaacatac
     5881 gatagaaccc acgcgcggtt gtgtccctac tgtataacca gctgatgcgg cgctcgcagc
     5941 ccaatcacca ccgtttcccc accagttacc tgcccatgtt gccatctcct ttacacccca
     6001 tgtgcattga cctagtggaa acgtattact agctggatgt gcttgagcaa tttcctccgc
     6061 acttgctagg tagtctacat ctactgcata gttatatgta ttcgcatctg catttgttga
     6121 agcaaatgaa aaagcggaca ccaaagccac tgttccaagc attgttttag actttaattg
     6181 ttttatgttc atgttttcct ccatctgatg actcctagtt tatcaaatcc atataacaga
     6241 caaatgaaaa cctgtaacaa gttttaggca attttttaca ataatttaca aaaaaactag
     6301 ttttagactt tacaaaataa agcctaaaac tagttataaa cattcttatg attaatataa
     6361 atctaggtaa ttatcaattt cccattgtga aacataagtc gcataacttg cccattcaat
     6421 acgcttagcg tctaagaaat tagtatagat atgagttcct agggccgctt ttaccacttc
     6481 atcttcttca agtgctttaa gagcattatg aagagtagat ggtaaatcaa caatacctgc
     6541 ttgacgacgt tcttctacag tcatagcata gatatttgtc tcaattggtt ctggtgcttc
     6601 aattttattc tcgattccct caagtcctga tcccaaaagt actgataaag caagataagg
     6661 atttgctgtt gggtcaaccg agcgcaattc taggcgtgtt cccatacctc gagatgcagg
     6721 tacacgaata agtggcgaac gattgcgacc tgcccaagca atgtaaactg gtgcttcgta
     6781 tcctggtacc aaacgtttat aagaattaac agttggatta atgatagctg tgtaattgta
     6841 agcgtgtttc attaacccac ctaaaaagta gtaggcatcc tctgataatt gcattccacg
     6901 tggatcttct ggatcaaaga aagcattgtt cccttcatta tcaaaaagag acatattaca
     6961 atgcatacct gaaccattga taccaaactt aggttttgcc ataaaagtag catataaacc
     7021 atgcttacga gcaattgttt taacaaccaa tttaaataat tgaatgttgt cacaggcttt
     7081 taaaacatca tcatatttaa agtcaatttc atgctgtcca actgctactt catgatgact
     7141 agcttcaact tcaaagccca tttgtgttaa gacattaaca atttcacgac gtgtattatc
     7201 tgctaagtct gttggagcta agtcaaagta gcctcctttg tcattaacat caagtgttgg
     7261 gttaccattt tcatccattt taaataggaa aaattcaggt tctggtccta agttaaatga
     7321 cttatagccc atctcttgca tacgtttcat attacgctta agatttccac gtggatctcc
     7381 ggcaaaaggt tctccttctg ctgtatagat atcacagatt agacctgcaa cagctccatt
     7441 ttcatctccc caaggaaaga caatccaagt atctaaatca ggatataaat acatatcaga
     7501 ttcattgata cgaacaaagc cctcaataga agatccatca aacattgctt tatttgataa
     7561 aactttgtct aattgttcat ctgtagccgg aatttcgaca tttttcatta ctcctaagat
     7621 atctgtgaac atcaaacgga ggaaggtaac atttttttcc ttaacttcgc gacgaatatc
     7681 ttctgctgtg atagtcatat agtctcctta atatctagct tgactgcatg tttacaaatg
     7741 catatttcca atgtgctgtg aaggacttga aaaacgccct tgattacgga gctcatcttg
     7801 taaaagtcga cgaacctcgg catctgataa tgacttttgt ttctctttgg acttatgttg
     7861 tctttgcatg tattcatttt taatatcaga aatatgcaaa ccatcgctaa tgaagtcttt
     7921 tatttccagt aagcggtcca tatcattcag tgaaaacatg cggcggttac cttcagtacg
     7981 ttcaggtgtg attaaccctt gatcctcata atagcgaatt tgtctggcag tcaaatctgt
     8041 caacttcata acagctccaa tcgggaatac agccattgta cgcctaagtt ctctttcttt
     8101 catgatggcc tcctttcatt aaaccattat aaactttaaa aagtttgatg tcaactctca
     8161 tgtgagattt tatgacatca aactataatt tgttattttt tagttttttc cgaagtattt
     8221 caatatctgt attaaccatc attgcaataa aaacaccaca aaaagtttca aaaatacgtg
     8281 caaaaacata tagaattgtc tctccgcttg ggatagataa agttataata agtaatgcgg
     8341 ctgttccccc aataatacct gatttattat tacaagcaac attaatcatg atacataaca
     8401 tggtacaaat aggaacaatt aataaagtga cccaaaaagc ttgatgaaat atctcattga
     8461 atgcaaaaaa tactaaggag agtaagccac ctatactatt accaataatt ctagaaaaac
     8521 caaagtgcac gcttttatca aagtcttctc ttaaactaaa tacagcagtt agagcaccaa
     8581 tttgaagccc gttccaacca aataaatgaa aaaccaataa aactaaaaag accgataagc
     8641 ctgttttaaa tgttctcatt ccaaatttaa actttttaat atcaaacttg taattagata
     8701 ataatttttt taacgttttc atacctatcc cttcactata tattctaaca tatcaatcca
     8761 taaaaccctt tttgtaaacg ttttatttat tactagtata tcttttataa accaatttat
     8821 atcaaatcgt cataaacttt tgaaaattgc agtgtcaacc aaataccact atatctaaaa
     8881 actagtaaat gataaatgaa gtcaaggaaa gttcaataac tctacattat caactaaaaa
     8941 aagacatgca tcagcatgtc ctttaatata aattataatg actattattt ttcagtcaat
     9001 gctgccaaac ctggtaatac tttaccttcg agcaattcca tgcttgctcc accaccagta
     9061 gagatccatg agaatttgtc agcacggcca aggttgatag cagctgctgc tgaatcacca
     9121 ccaccgatga ttgatttaac gcctggttgt ttaacgatag cgtccattac accgattgta
     9181 ccagcttgga agtcagggtt ttcaaagaca cccataggtc cgttccatac aactgtttta
     9241 gcaccagtaa gtgcttcatc aaatttagcg attgatttag gaccgatgtc aagaccaagg
     9301 aacccttctg aaactgcttc accttcagtg tcgcgaactt cagtataacc agcaaatgcg
     9361 tttgcttctt ttgagtcaac tggcaagatc aatttaccgt ttgatttttc aaggaggtct
     9421 ttagcaacat ccaatttgtc ttcttctaca agtgagttac cgatttcgat accttgagct
     9481 ttgtagaatg tgtaagtcat accaccaccg ataagaactt tatcagcttt ttcaagaagg
     9541 ttttcgataa caccaatctt atcagaaact tttgagccac caagaatagc tacgaatggg
     9601 cgttctggag tttcaactgc ttcttggatg taagcaattt cgttttcaag aaggaaacca
     9661 gctacagctt tttcaacgtt tgctgaaata cctacgtttg atgcatgagc acggtgtgct
     9721 gtaccaaatg catcgttaac gaagattcca tctccaagtg aagcccagta tttaccaagt
     9781 tcttcgtcat tcttagattc tttcttaccg tcaacatctt caaaacgagt gttttcaacc
     9841 aaaagaactt gtccatcttc caaagcattg attgcttctt ctaattttgc accacgagta
     9901 acacctggga atacaacatc ttgaccaagt ttagcagcta aatcagcagc taccggtgca
     9961 agtgattttc cttctttgtc agcttcttct ttaacacgtc caaggtgaga gaagaggata
    10021 gcacgaccac cttgttcgat gatatacttg attgttggaa gagccgcagt gatacggttg
    10081 tcgttagtga taacgccgtc tttcaaaggc acattaaagt caacacgaac gaggactttt
    10141 ttacctttca aatcaacgtc tttaacagtc aatttagcca ttagtaaaac tccttatata
    10201 atttgatatt atttattata acatattttt gtgaacagtt gtaattattg attacttttt
    10261 cacaaataga tttcatagat tttcctagtt gtttttaaga gaattcgtat acttattaat
    10321 catacgaatg caacattttt tgtcgttctt tcaattgttt ttgaacatcc agatgttttc
    10381 cttgataaat agcactttcc caagaaccgt acataggatt tgggaaaaca ataaatttac
    10441 taccaaactc actttgaagt ttagttagta gttgttctct atctgtacta gatgatttag
    10501 aaaaatcagc aaaatcaact aaattatctc caaaaagcat aattaaattg gtatcttttt
    10561 gaactgcctg acggcgactc tctttagatt tcattccttt tttaaggaaa agcaagtggt
    10621 ctttcccttg aacaggtata ccttccttct ctaaattttc tttagtcgca tcaacttgag
    10681 catctgtacg atctgagaca taataaattt ttattccctt ttcattagca tacttcaaaa
    10741 attctttggc acccgcaaca gccttagctg atttcttttg cacccattta tcccaactct
    10801 ctggcgtgaa actagagcca tctttaatat tctttgcttg atatgggcta ttatctaaaa
    10861 ctgtttcatc taaatctaag ataattgaat atggtttttc actgggcttt tgtaaccaat
    10921 catctaactt cattttagca acattataac cttgtaaata taaagccttg gcttctgcag
    10981 aattttgata ccaaaggaca gacatagtgt tttccttagc caacagttta ttagaactta
    11041 gagaaatatt tttaaatttc gtcttttgct ccaccttatc tggagacata tcagcttttt
    11101 tagtactgtg gtttgactga catgctgtca tcataaatac agcaacagat aacgataaaa
    11161 ctgaaaaaac ttttgtagtc ttcataaaac ttctcctcta tattttttca attaaatcat
    11221 accactgctt actttcaaaa tcaaacctta ttttaactta tagtcaatat aattcatttt
    11281 tcatttattt tctgataatt ttctataata aaatgaaaaa acattcgtat tattttttat
    11341 ttataaaaat aaaagaggga taaatccctc ttttacatca tattaattaa aaattatttt
    11401 gcaatttttg caaagtattc aagtgtacga acaagttgtg aagtgtatga catttcgtta
    11461 tcgtaccatg aaacaacttt aaccaattgg ttaccgtcaa cagtttgaac tttagtttga
    11521 gtagcatcaa acaatgaacc gtatgaaata ccaacgatat cagatgatac gattggatct
    11581 tcagtataac cgtatgaatc gttagctgct gctttcatag ctgcatttac ttcttcgaca
    11641 gttacgtctt tttcaagagt tgcaaccaat tcagttactg atccagttgg aacaggaaca
    11701 cgttgtgcag caccatcaag tttaccgttc aattctggga taacaagtcc gatagctttt
    11761 gcagcaccag ttgagttagg aacgatgttt gcagcacctg cacgagcacg acgaaggtca
    11821 ccaccacggt gtggtccgtc aaggatcatt tggtcaccag tgtatgcgtg gatagtagtc
    11881 atcaaacctt gtttaacacc aaagttgtct tgtaaagctt tagccattgg agcaagacag
    11941 tttgtagtac atgaagcacc tgagataact gtttcagttc catcaaggat atcgtggtta
    12001 gtgttgaaaa caactgtttt aacgtcgttt ccaccaggag ctgtgataac aactttttta
    12061 gcaccatttt catggatgtg ttgttcagct ttttcttttg atgcaaagaa accagttgct
    12121 tcaagaacga tttctacgcc atcagtagcc cagtcaatgt ttgctggttc gcgttcagca
    12181 gaaactttaa caaattgacc gttaacttcg aatccacctt ctttaacttc aacagtaccg
    12241 tcgaaacgac cttgagttgt gtcatatttc aacaagtgtg caagcatgtt tggatctgta
    12301 aggtcgttga tacgagtaac ttcaacacct tctacgtttt ggatgcgacg gaatgcaaga
    12361 cgaccgatac gaccgaaacc gttaatacca actttaacta ccattagtga tttcctcctt
    12421 atgaaaatct caaatatttt attttaggga tccccctaat caatgtgaaa agattaactt
    12481 gtacatatgt gcaaaaatct ttcaacacaa ttattatatc ttttttcgag aaaaattgca
    12541 atgaatttac cactttcaaa aagaaaaaaa gatactcttt cgagtacctt tcataagttt
    12601 tcagaaatta ttcaccagag tttttcttga tgatttcttc ttgaactgac ttaggaacat
    12661 cttcgtagtg gtcaaatacc atcatgaatg tgccacgtcc ttgtgttgca gaacgaagaa
    12721 ctgttgcata accaaacatt tcagcaagtg gaacaaaggc acgaacaact tgtgtattac
    12781 cacgtgcttc cataccatca acacgaccgc gacgtgcagt cacgtgaccc ataacgtcac
    12841 caagattgtc ttctggagct gtaattgtaa caagcatcat tggctcaagg atagctggtt
    12901 gcgctgattt agcagcttct ttaagtgcaa gtgatgcagc aatcttgaag gctgtctcag
    12961 atgagtcaac atcatggtat gaaccatcgt aaagtttagc ttttacgtca accattggat
    13021 atccagcaag aacaccgttt gccatagatt ctacaagtcc tttttcaacg gctggaataa
    13081 attcacgagg aaccacacca ccgacgatag cattttcgaa ttcgaatcct ttaccttctt
    13141 cgtttggtgt aaattcaatc caaacatcac caaattgacc tttaccacca gattggcgtt
    13201 tgaagaatcc acgtgcttgt gtagaagcgc ggaatgtttc gcggtatgat acttgaggtg
    13261 caccaacgtt tgcttcaact ttgaattcac gtttcatacg gtcaacaagg acatcaaggt
    13321 gaagttcacc cattcctgag ataactgttt caccagtttc aacgttagtt tcaacgcgga
    13381 acgttggatc ttcttcagca agtttttgaa gggcgatacc cattttatct tggtcagctt
    13441 tagattttgg ctcaaccatc aattggataa ctggttctgg aacttcaatt gattcaagaa
    13501 taacttttga tttttcatca gtcaatgaat cacctgtagt tgtatctttc aaaccaacag
    13561 ccgctgcgat atccccagca taaacagttt caatttcttg acgactattt gcgtgcattt
    13621 gtaagatacg tccgatacgt tcacgtttac ctttagatgt attcaacacg tatgaacctg
    13681 aattaagaac acctgagtac acacggaaga atgttaagcg acccacgaaa ggatctgtca
    13741 tgatcttgaa ggcaagcgct gcaaatggct cttcatctga agcaggacgt gtttcttctt
    13801 cgtcagtatc agggttaata cctttgattg caggaatatc aagtgggctt ggaaggtaat
    13861 caataacagc atcaagcatc aattgaacac ctttattctt gaaggctgaa ccacacaata
    13921 cagggtagaa ttcaacattg atagttgctt tacgaatagc agccatcaac tcttcgttag
    13981 tgatttcttc accttcaagg tatttcatca tcaaatcttc atcagtatca gcaactgctt
    14041 ctactaattt ttcacgatat tcatttgctt gatcaacata ctctgctgga atatcttctt
    14101 caaggatatc tgtaccaagg tcattagtat agatttcagc tttcattttg attaagtcaa
    14161 taataccacg gaaatcgtct tctgaaccaa ttggcaattg aattgggtgt gcattcgctt
    14221 gaagacggtc atgaagtgat tgtactgaat agaggaagtc agcaccgatt ttatccattt
    14281 tattagcaaa aacgatacgt ggaactccgt attctgttgc ttgacgccaa actgtttcag
    14341 tttgaggttc tacacctgat tgagcatcaa gaactgttac cgcaccatca agtacgcgaa
    14401 gtgaacgttg aacttcaatt gtgaagtcca cgtgtcctgg tgtatcgatg atgttaacac
    14461 ggtgaccatc ccattgtgct gttgttgcag ctgatgtaat tgtgatacca cgttcttgtt
    14521 cttgttccat ccagtccatt tgtgaagcac cttcgtgtgt ttcaccgatt ttgtggattt
    14581 taccagtata gtaaagaata cgctcagttg ttgttgtttt accagcatcg acgtgagcca
    14641 tgataccgat attacgagtt ttttcaagtg aaaattcgcg agccatttgg tttctcctat
    14701 attttaaatt atttacttct ttattataac actatttata aaaaacggat aggcaggacc
    14761 tacccgttct aattgctaac attgctatct accaaacaat ttgtttagta gctatgataa
    14821 gtctcaaaaa gagagtaatc ttaccaacgg aagtgtgcaa aggcacggtt agcttcagcc
    14881 attttgtgag tatcttcacg tttcttaact gatgctcctg tgttgtttgc agcatccatg
    14941 atttcttttg caagacgatc tttcatagtg tgttcaccac gagcacgtga tgcgttaacc
    15001 aaccaacgaa gtcctaaagt tgtacgacgt tctggacgaa cttcgacagg aacttggtag
    15061 tttgatccac caacacggcg tgcacgtacc tcaagtacag gcatgatgtt ttccatagct
    15121 gtttcgaaca cttcaagagc atctgttcca gtagcttctt taattgcttc aaaagcatca
    15181 tatacgatag ttgcagcagt accacgttta ccatcaagca ttacacggtt gataagacgt
    15241 gtaacgattt ttgagttata caatggatct ggcaatactt cgcgtttagg cgcttgattt
    15301 ttacgactca ttttctctta tcccccttct aattaccctt taggacgttt agcaccgtat
    15361 ttagaacggc cttgtttacg atcagctaca cctgcagtat caagtgcacc acgaacgata
    15421 tggtaacgta cccctggaag gtcttttaca cgtccaccac ggataagcac aacactgtgt
    15481 tcttgcaagt tgtgtccgat acctggaatg taagcagtaa cttcaataag gttgctcaaa
    15541 cgtacacgag caaatttacg aagggcagag ttaggttttt taggtgtcat agttccaaca
    15601 cgagttgcaa caccacgttt ttgtggtgca gataatttag tgtgaacttt tctgtgactg
    15661 ttgtaaccaa tattcaaagc aggtgaatct gatttttcaa ctttagattt acgtggttta
    15721 cgtaccaact ggttaattgt aggcatctac atttctcctg taagtttttt atttttggtt
    15781 gatatagcac ttggtgacag cctatatctg cgtgtacttt tgcaacaatt gtcagcacgt
    15841 ctctgtacac ttttgagaga ccaaaagtaa aagtaccgtt tatcatagta tcattttaca
    15901 cataatgtgt caagctttta ttacttttac aagcaaaaag aatcaaggat gtcaccttga
    15961 ctctttaatc aaatatattc cccgcttcta cagagactct attatttttt acatcaattt
    16021 cagatactct cagaagagat ttataggcca tcttctcacg ttgttcctga gcattttctg
    16081 caaatacagc gacacctact aatgtgctat cgaattctga gagtaaacta atcatccccg
    16141 aaacagtacc tccacctttc aagaaatcat caactattaa aactcgactg ttaggtttta
    16201 agctacgctt agaaagaaac attttttcaa tacggccact tgaaccacta gcgtagttta
    16261 ctgacacagt agaaccttcc gtaatcttca aatcacgtct cacaataaca aaaggaacat
    16321 ctaaaacatt tgctactgcg tttgctaaag gaacaccttt tgttgcaacc gtcatgacag
    16381 catcaatttt ttgacctctg taagcgttcg ctataatgcg accaatactt tgtaacattt
    16441 ttggtgtact gagtagatct gaaagataaa tgtaacctcc cggaagaata cgatcgtttt
    16501 cagataaacg ctgtcttaat tcttcaacga tttctttcgc ttctgcttca gcaatagttg
    16561 gtgtaaagat aacacctcca cttgcaccag tcacagtttt aatatctccg atttgagctt
    16621 gttcaaaagc ttttttgata atagcaatat cttctgaaat cgaagatttc gcggcaccgt
    16681 atttactagc aaatgtattt aaactagtta atgtataggg attatttatt aggtaatttg
    16741 aaataacaac catgcgttcg ctacgtctta atttcatatt ttctccttat tcatagcatt
    16801 ctttacatat agtataacat aacaaatgct aaaaacgaac attttatttt aggtcttaat
    16861 tattctacct aaattattta atgttcggtt tataaaaaga acggttatcc atggcaaaaa
    16921 tgcgatttgt catttcacct tcgttgacac ggtttaaagc tgttgtcatc atcatcatat
    16981 ttgcatcaat gttatcgatc atatggataa tttctgcctc catgattcta ggacgaaccg
    17041 ggcttccata ttctaattgt ccatgatgac tcagaataac atggcgtaac actgtaacct
    17101 cttcttttgt atcatcaata ttaagctctg ctagtatttt tgttaattcc tcatcaataa
    17161 gtgaaatatg accgataaga ttacctcgaa tagtatattc tgtattatca ggacccgata
    17221 actcaatgac cttggctaaa tcatgtagca taataccagc aaacatcaaa cttttattaa
    17281 gttctggata gatatctccg atactatctg ccaaacgaac cattgttgcc gtgtgatatg
    17341 ccaatcctga ttcaaaagcg tggtggtttg ttttggcggc tggataggta aaaaattcct
    17401 tattgtattt acgataaagt gctcttacca cacgctgcca ggttgcattt tcaattttaa
    17461 agagcatttg ttccatgtac tctctcacgt tgtcaacatt gattggaggt ttttctttaa
    17521 aatctctagg atcattaggt tcaccatctt tgatgtttct taatgtaatt tggttgactt
    17581 ggggagttcc attataaact tcacgacgcc ctttcatatg aacaatttta ccggctacaa
    17641 actcttctac attataggtc tgtgcatccc acatatttcc tgaaatttcg ccagtatcat
    17701 cttgaaaggt aaaggcaata aagtctttac ctgcacgcgt tttacgaact tctgctttct
    17761 ttataaggta aaacccctca aaaagctcgt cttttttcat ttggttaatt ttcattaatt
    17821 tcctcttctt ctgttatagg aaggtttaac aagctagtgg ttgcttgatc ttgatgttct
    17881 tcaactgtat ttaagactct gataatagcg tttgtccgcg ttgtgagtaa actatcaata
    17941 cttttacttg ctgtatttaa ttgtttttga gcttttgata acatgccacc aaattttcca
    18001 aattcaacct tgacattccc taaaatttta ctaatatcat tagcattctt ttgaatattt
    18061 aaggttttaa agccaacaga taatgaattt agtaaagcag ataaggttga cggtccagct
    18121 actacaatat tttcgtcacg tcttagacta tcaaagaatg ttgcatttct taccacttca
    18181 gaatagagcc cttcagttgg taagaacatg ataccaaaat ttgtcgtttc aggaggattt
    18241 aagtacttat tgtttatatc ttttgcaaat ttacgaatac ttgccagtaa agatttacga
    18301 tagagttcta tttgaacctt gtcacccaat tcataagcat cttccaatcg gtaataatct
    18361 tctagaggaa acttagagtc aataggcaaa tagatataat ctccctgacc atttccaggt
    18421 aatttaatag catattcaac acgctcagaa gagccagaca ccgtaggaaa ttctctctca
    18481 tattgactaa ctgtcataat atcttctata atttggccga gttgtaactc ccctaatatt
    18541 ccccttgtct tagtatttga caagactttg ttcagtgtac caacatcttg ggcaacagtt
    18601 ttcatctccc caagaccttg attgacgctc tctagttgac gcgatacagt ttcaaaagaa
    18661 gtttgcaaac gcgtttgtag cgttttttct agcttttcct caacagtttg gcgcatttga
    18721 tccaagcgtt tttcattgga atcttgcatt ttctgaactg actgagataa attggagtta
    18781 atcaattcca agcgtttgtc tgatcgatct cgactctccg ttaaatgctg atttaattct
    18841 gtacgcatat cggtaagcaa ctgataaatt tcctgttgca tacggtttag ctgattagtc
    18901 aacgttagaa tttgatcttt ttgcgccact tctatctgat atgtcagttg gtctgataga
    18961 ttatcagcat tatcttcaag ttgtttttca acggactttg ttaattccgt catttttggg
    19021 taagaaatta tcgtcactgt cagactagct agtacaatga tcaaaatgat gacaagcata
    19081 ttaacctcta tccttactat aaataatgat taagtaacca tctttgagct ctacttcaat
    19141 tggcgaagtc ataaattcat tactggaata catttttttc ttaaaataat tacttttgtt
    19201 caattcatat ttagccccag aaatctgcaa agtagactca ctctcaggca tgaacgagac
    19261 ataggacatc ccttcaatcc ttgagacttg atttttcccg gatggtagat aagtcactat
    19321 gttttgttca tctttgagtc tgatttgtga cataaatggt tctagatctc tgtcacttgg
    19381 taaaaagata ttagacatca tatggtcaat cctgccacca aatgctccaa atacggtcac
    19441 ctgagcttct ggaaattctt tgaaaatagt ttttaaagct aattctgtat ctgtatcatt
    19501 tttttctgca ctagcagaaa cgatattgct acaatagtgt ttgatataaa gcagttcatc
    19561 ttctgttata gaatcaaaat cacccactgc catgtccaaa gaaagcccat tttttaataa
    19621 aaataggctt ccacggtcaa ttccgacaaa atagtcaaaa tcatattcaa aataagttaa
    19681 gtcaccgcca gcaaataaag caattttagt catcgagcgc cacccttaag gtttctactt
    19741 gtgcaggtaa atcacttgct ttaaagagat aagaacctgc aacaaagaca tttgcaccag
    19801 cctctgctgc tgccttaatt gtagtattat ctataccgcc atcaacttca atatcataat
    19861 gatgaccgta ctctttgcgc catgctgcaa ctgtctttac ttttgacatc atttctggaa
    19921 taaaggcttg tccaccaaag ccaggattaa cagtcataat taaaatctgg tcaaccaaat
    19981 ctaagatagg aataagagat tctacgggtg ttcctgggtt aataaccaca cctgctttca
    20041 ttcctgcctc ttttatcttt tgaagcgcac catgtatatg tttcgttgct tcaacatgaa
    20101 tggtcatgat atcagcacca gcttgagcaa aagcctcaat ataacgttct ggatcaacaa
    20161 ccatcagatg acaatcaaaa acaagtttac tgtgtttacg catgctggat acaacatccg
    20221 caccaaaact aatattaggg acaaattgac catccatgat atcaatatga acgtattctg
    20281 ctgttgtctc ttcaatacgt ttcaattcgt tagcaaaatt agcatagtct gctgctaata
    20341 tcgatggggc aattttattg gttgacatag aaacctactt tcttttaata actttcttat
    20401 atgtttcacg acggttctca atttcactta aaaattggag gtagttatca tagcggactt
    20461 gccataactg accttgagtt aaggcttctt ttacagcaca tttaggctca tgtgtatgcg
    20521 tacacgagcg gaatttacaa aaatgactaa gtctacgaag ctctggaaag gattcgttta
    20581 agtcctctgc attatccact tcatagtcca aagaagaaaa accaggagtg tctgcaattt
    20641 ttcctttatg aacgttatag aaactaactg ctcttgtggt gtgtcggcca cggcctaaac
    20701 tacccgaaat ttcaccagtt tcaagtttca actcaggagc aattttgttg agaagcgttg
    20761 attttccgac acctgtctgg cccataaaaa cagtcacttt atttgctaac agaggcaaga
    20821 gttcctcttg agaataacag aaaacgtagc ctatattctg ataaagttcg cgaatatcat
    20881 caataacaac caaatcgtct aacaaatcca acttggaaat ataaatgata ggctgaatca
    20941 tcttatattc tagaagtact aagaaccggt ctaataagtt agcgttaaaa tcaggttctt
    21001 tagcagacat gataacgaca gcttgatcaa tattaacaat aggtggtcgt acgaggctat
    21061 ttttacgctc ttcaattgat agaatatatc cttctgattg atcttgcgaa gaaaattcaa
    21121 cccaatcccc aacgtaggga atttgacctt tttttctaaa atttccacgc gcacgtgtct
    21181 gataaacaac accgtcagat tccacataat aaaaacctgc tagcgacttg acaattctac
    21241 cttgcaattg ttctccttaa ttgtttagaa taccactatt atatcataaa atgagaaata
    21301 agctctactc tcagagataa gctaaataga aactatttgc aggtgttcga tttctctcaa
    21361 ataacctcaa ttttatgaat tcccaacata aaagcagtga tcttatttag atacactgcg
    21421 cttttttcca attactatta aggcacctac tgtaatttct gatatactcg cccaatcaat
    21481 agtcgtttta tctacgctga ataacaacgg tgtcatatca ataaaagctt gacgttcttg
    21541 ttgagaacag ttatagcttt gaacaacttt ctgctcagaa ataatttcaa aattttctct
    21601 aaagtgatct agaatttttt gattagagta atctttctgt ttcaagtatt gactagcttt
    21661 ttctcgcaat tcttgaacat gttcagctac aggaacaacc tttacaagga taccatcatc
    21721 actcagcacc cgcctaaatt ctttataatt tgcaggagaa aaaatgtcta aaatgatatc
    21781 aattgatgag tcttgaatag gaaggttagc taaatcagaa acaaaccatt tcacaagcct
    21841 agactgatca cttttagcag ctaattgaat cgaatcttta gaaatatcaa aagccaagaa
    21901 agtcttctca tgcttgttaa ctaattgacg agaataaaag ccctctccac aaccaatatc
    21961 taaaaccgaa tgaaactgag aattattttc caaaaccttt gaaatagctt ctaaaatatg
    22021 attataatac ccgttttcta agacaagctg ccgattttca aatgattttt tatcataatg
    22081 ttcatctacc ttctttcctc caagaagatt aacatatcca aatttggata aatcaaaaca
    22141 atgattatca gagcatttga ggctgttact ttcttttatc aagggatttt gacatttagg
    22201 acaagcgaaa taacttgcac tatcttcaaa acgccacttc ttgtcaacca acatagttta
    22261 tatcccaacc tctttcaagg catctgctaa acttgcaaaa tcagggatag ataaggcttc
    22321 tccgcgaata gatggcttaa tttttgctat ttctaatgct ttttccaatt ttgctttagt
    22381 atcttctgat tttccaaaat gactcgttag attattccaa agtgttttac gacgatgtac
    22441 gaaggctact tttgacactc tgaaaaagaa atcctcatct tgaacactca ctactggttg
    22501 atctctacag accattttca aaatagctga atctacattt ggagcaggaa caaaaacagt
    22561 tctcggaacg ataaatgata ctttggcagt catataatac tgcactgcga ttgacaaaga
    22621 accgtatgct ttagtatttg gcatagctga aatacgatct gccacttctt tttgtatcat
    22681 gaccacaaac tccgcaaaag gaatcttgct ctcaatcaag tgcataagga ttggtgttgt
    22741 gatgtagtaa ggtaggtttg cgaccacctt gataggcaga tcaggattct tgaaagcctg
    22801 aatctgggtc tgcaaatcag ccttgaggat atcctgattg accacttgga cattgtcaaa
    22861 acgcgcaagc gtatcagcaa ggataggaat gagacgatca tcaatctcaa aagccataac
    22921 ctcagctgca ttctcagcca aaaactctgt caaagccccg ataccaggac caatctcaat
    22981 cacgttgaca cccttgtcaa tctctgccgt gtcaacaatc ttctgtaaaa tattagtatc
    23041 ggtcaaaaag ttctgaccga atgatttctt aaatgtaaac ccatgacgct ccaaaatagc
    23101 gcgggtaacg gttttatctg cgattctcat aattcctcta tttcttaata ttgatcattt
    23161 ttgaaataac atttgaggct atttgtgata ttacaactgg attataccac tacttccttt
    23221 tgcctttact attctgagtt ataaacttat ttttaattgc agttgcacaa gcttcggtta
    23281 gtggttctcc tattgcttgc ttataattaa atccaatcaa ctgcattgtt tttaaagtat
    23341 ctttattcaa taatgtaacc aattgtttca ttgttcgttc ctgttcctca tttagaccag
    23401 aaaaattata taacaatgag attccttgct cgagagtatt ggatagatat tcaatatcct
    23461 catcatctat attaattttt tcatactctt ctttatatga ctgtgcaatg cgaattgctt
    23521 gctcccgttc agctaataat tcgttaatta aatcatcata agtagctctc aatgttgcag
    23581 catcttttac atcctttatt gattgaattt tactggttac catagttgca gtccctttga
    23641 ctgcaagcgt tgctaaatct accccagctc tagtcaatgc ttccgttaca ttatactcca
    23701 ctatttttct ccttttctat taatacccct ccatacactc ttcaacttct gccttggtca
    23761 ctccgaagag tctgaggcgt ttgaggagtt gtttaccatt tgagtagccg atacggagtt
    23821 gattcccaag atactcacgg cgttttcggc tatcgctagc agtaatgaat ccccaacgaa
    23881 tgaggtctgc ttgggtgata tcgaaatgat cttcatcatc gaaatgctga gtcacttttg
    23941 agagtgcctt ctgcaaatct tcaaagctgg catgctcgac acccagagat cgtcccttgg
    24001 tctttgagcc tggctttgcc tcgtctcgat tgaggaaggc atggcggact gtcggaatgg
    24061 cattcatgat gattttacgg atacgctcgc cattataatc tgggtctgta aagacgataa
    24121 ctccccgcaa attgtgcaaa cgttcgatac gctctaaatc atcttcatca attgctgaac
    24181 ctctagtttc ataggtatca acattgtaaa agcgtcgcag attagcagta tcatctttcc
    24241 cttcgacaac gatcacttct tgaatatcaa tttttttcat caagtctaaa tatccttact
    24301 gcattttggt atgtagcctc cgctacctct tccacagtta tgcctcgtag ctctgctatt
    24361 ttttcaacga catatctcgt ataagcggtt ttgttttctc gacctctttt aggtacagga
    24421 gccaaataag gagcatctgt ttccactaat atcttatcta atggcaactc tcgtgcagct
    24481 tcttgtacat ctaatgcctt cttaaaggtc acaacaccag aaaaggaaat catcatccct
    24541 aaatcaataa atttctgagc catttccaaa gaaccagaaa aagagtgcat tataccacca
    24601 aaaggcccaa ctccagattc cttaatgact tcataagtgt cttctaacgc atcacgtgta
    24661 tgaacgacaa aaggaaggtt atactctttt gaaagttcaa tttgacgctt aaacacctcg
    24721 atctgaatat ctttgggatc ctccatccaa taataatcca gaccaatttc accaagtgca
    24781 ataaccttgg gattttctaa atgactgata atcattgact caatattatc atcataagaa
    24841 ccagcttcag ttggatgcca tcctattgtg ctatatacct gtgcatattg ggaagataat
    24901 tccaaagatt ttgaaatggt atcttggtca aagccgacaa cattcatttt tgtaacaccg
    24961 agttcgctag ctaaattaat ttcctcgtct atctttcctt caaaattttc tacgttaagg
    25021 tgggtatggg tatcaaatat ttttatcata tcatcgttct ttctttgctt atcgtagcta
    25081 caagcattaa tctttattat tataacataa gactgaagca ttctgatcag aaagagtaaa
    25141 tcaaaaaagt caaaacattt ttgctctgac ttttgaatac ttgctgtaaa aatcctctac
    25201 aagtgtctag ctaatggtag agaaactaga ctataaatga ttatattttt gctgattgct
    25261 ttcaatagcc tcctcaacct ttgaacgaaa ttctcctttt attgcctcgt ccaattgatt
    25321 agcaacttta tgaatagctg tctttgtgtt gatggctact tcgtctattt ctttatgatg
    25381 cgacgtaata atatcgtgaa acttagatct cttgcgtggc gcgtacacgc tatgtatcaa
    25441 ttctttatcc aattgttttt ttaaagtcct tactgactcc ttaccagtca ggacatccca
    25501 cattaaactc aataatttct cctacttcat taattctctt ttcttttccc tttgcaactc
    25561 ttcaaaggtt agttcatgtt ctgaacgttg ccgaatatag ttagctctat attcttcttc
    25621 ttgagcggac aaggcatagt actctttatg gtaagcttgc tctaagtcgt catgataaga
    25681 acctaaaata cgattgagcg tcatcatatc tgagctatca ttactatatc tcaaatcata
    25741 ttcaacagcg tcgtaagttt cttgaagcat attaataatc ttattttttc ttactgagag
    25801 atcgtctaat ttttcttcaa actggcaaca actatggtga taggcatcat ctaaatctga
    25861 tatttttctc tgagtatcag ctaattgttt ttcaatgata tcaatcagtg ccattgttta
    25921 aactcccctg acaagttact atcttgtcgc aacatagctt caactccttt attaatttgg
    25981 ttttctaatt caacgtaagc gtgagaaatg ctttctgccg cttgagtttt atttgagaaa
    26041 aaggtttgag cttctccgac aatgctcgca tagctaacac ctgactgagc atacgcttct
    26101 tccaattctt gaggacttag ttcagtaact cctgtaggag ctacttttaa agattgataa
    26161 agctcttctg ctttagcaac tgctttttgc tcaacttttt taacttcttc aactgcttct
    26221 tttgatatcg ttaccaaacc actagagatc acttttgctt gttgactatc taggaaaata
    26281 gcttgattct tactaacttc ttttcccgta cttttaaagc tactaagttt cgctttaata
    26341 gcatgcattc tttgattcac attcgtatga actactgaat agttttcttt gtaagcaaat
    26401 ttatcttctg ctaattttaa agaaccatct ggattgtact ggtagcctcc ccaaatatgc
    26461 tgattggtaa aattagcaac ccagctacct cctgaaacag ctttactgtc aactatttta
    26521 acaattccga cagcattatc gcttccttct ttaggataac ctattccaat tttatccttg
    26581 gaatcaacgt aattattgac cacccctttt attttatcaa cctgttcttt ttgtttatct
    26641 gtcaaaatac tataaaggtt tggaccgtta tatatccaag ctccttgaac tcggtaaatt
    26701 tgattatcat ccaattctgc aatagcatac tgtgcattca ttgaccctaa cgagtgtccg
    26761 tagaccgaaa tttttgcgtt aggatattta ttcataactt cttttagcag atgagctgat
    26821 tgttctaact gctttgtacc ttgtttatga gatacacttt gtgaaccaaa tttctttaat
    26881 aatagtttac tagctattgg aatatcattt ttaaaccagt ctgttttcca atcatcccct
    26941 ccaaacggtg ctgttgaccc cttaaataaa accgtaactt ccttaacatc ctctggcttg
    27001 tctaaagttc catttggagt gatggtataa atttgttccc cggtttcttt gcctccatct
    27061 tgaacgtgag caactttacc gatggggagt accttatctt ggctcattat tttgactagt
    27121 gtatcaagtt ggctacctaa ttcatactct tgcttcgcta tatttaccct atctaaatca
    27181 gtcaagactt tttccattaa tgacctctac tttccaataa atgagttaac tggggagatt
    27241 gatggctact ttgactgacc aaatgccctt gattatccct atccaaatta caagtaatat
    27301 ataagttagg atctttatta atgattaacc ttaccgaaat ccctcccata ggattttttt
    27361 tgatactttc tttgtcaata tcatacgact taataacagc attctctttg aaagcatctt
    27421 catcttctgc ataaatccac ttttgataag catttttagc gtctttgcta ttagctattt
    27481 cccataattc ttgttgttta ttgttttcag tcacttttaa acctcctaca gtgaaaccaa
    27541 gccctaaaag gattattaca ataaaactaa gtaattttat tgacttcatc tctcctcctt
    27601 ttatcatata acactcattt gatatctagt ttatcataaa tttattctca ttataaatga
    27661 aaaactataa aacatggatt agttatctta ggattaccaa atacaaagat attaaaaaag
    27721 atgattaata gctctaatgc tataaatcat ctgactctac tatttacttg taatcacttg
    27781 gacgttctgt atattttgcc caagatttct tcaaaaattt attattaatg tgataattcg
    27841 gcttgctgtt ttcctctgtt aaaaatggat tgatatgctt atctagctct aaccaaccat
    27901 tccaacctaa atggattgta tcttgcataa agtatggctt acctccatct cgagaaaggt
    27961 cagcgatatg attgaaacct tgactttgaa gttggtgttt tattttttct acagattttt
    28021 gatacatttt ttgatcaagc cctgtgtagt ctgtccactt tttatttact ggtggtatga
    28081 caaaaaggac atctgtattt tgttttgaga attcattaag aaccaactgt aaatcattaa
    28141 attctggtga cttagtataa ttgaaatgtc gttgtgaccc tttcaatttt tttaagtgct
    28201 ttttaattct tgtttggtaa aagcgatcat caataccaaa ttgatttgat ctcgttgcta
    28261 ctttagcatc ttgtgatgca atacgcgata aatgattata tgagaatgct ttcggtaatt
    28321 taccaacatg aggcattatt gcctgattat aagtttttcc tagtggtaga tagctaaaaa
    28381 tagcatctgt cttctcaaat acccaatcat taaactttag tctttgtttg tctttatttg
    28441 atagtttttg gccatctgca actttttcta tcaaatctga catagaagct tcaggatata
    28501 attttaacaa tcgacgagca gcatacctat cgtaggttgt ccctgtttga ttttgcaaaa
    28561 atcgaatggt ttgatcgttg ctgaaatagt tttgaaaagc acctttgttg gctcccttgc
    28621 gaacaaacca ttgcggagaa ataacataga tagctttttt atttttgatt tgtcctttaa
    28681 tctgttgaat gccatagtat tgtgtcagag aagccgcccc tttttgacct aaaagatatg
    28741 ggatataaga cctattgtaa gcctctgcta aaactgatgg atgcattgcg tcaaatctta
    28801 gccactcact tgaaccaaag aaagggacaa aattggcttt aggatcactg aaagctctaa
    28861 ctttttgaac tctgctttta aaacctgcct tcgtcaacgc tactgcactg tttttttcct
    28921 tacctaaatg atgaggctga gaaacaggaa aaacaaaata taatcctacc aacaatagta
    28981 aagcacaaac tagaggtcca aatactttac ctagacgttt aagcattgca gagctccgtt
    29041 actccagcaa caattttatt agctgtgttc cagtcattac gaccaaattc cgcaatcgga
    29101 atatcaatat taaagtggct ttccaactca acaattagtt caacagtacc catgctgtct
    29161 agaactcctg cgtcaaataa gtcttcatcc atcatagaag aaacatcctc cataaatagg
    29221 tcatcaataa ttgctaatac ttctgattta atatccatta taatactcct tattatttgt
    29281 tatgcggttg tgggaaccac aaaagatcta aaaatcctga aaaaatcaag aaagaaaaca
    29341 taactacatt aaaggtgatg aagacaccca aagcataagt ccatttgttt tctggtaaaa
    29401 gtggtttttt agccaatctt ctttctttat taatattttt cttcttacgt acccaagcgt
    29461 cattaataac taggccaatc ccatgaaaga gaccataggc tatatagtac caagttaacc
    29521 catgccagaa tcccataaga agcatattga taatataagc tacacttgaa gtaacattac
    29581 ggtttttaaa aactttattt ttaactaaaa gcttaacaag cctcatgaaa acaaagtctc
    29641 taaaccagaa agaaaggctc atatgccatc tattccaaaa ttcttttagg tcgcgtgatt
    29701 tgaaaggttt gtcaaagtta atcggagact taatccccat taggttagaa atagccaacg
    29761 caaacattgt gtaacctgca aaatcaaaga acaaatcaaa accaaaggca tacataaccc
    29821 caagtgttgg ccaattaaac acaccaccct gttctagcgc taattctttt agaggaggca
    29881 taatcagact tccaaaaata taggctaaaa taaacttata gagaaaacct aacatcaaat
    29941 aatgaatcgc ttgacctaac atatctagga gttcgtttcg atctgggata ttaatgtaat
    30001 cctcattgaa tcgtttaaaa cgatcaattg gtccacttga gaaagttgga aagaagagta
    30061 aaaatcttaa gaattcccat aaagtaaatt ctttcaagac accgtctctc atttcaataa
    30121 tcatagcaac agctctaaaa gtaaggtaag aaattcctag aaacccaaaa agtgactgat
    30181 gcccatcctc agatatagct ggaattaatt ttaccaaaaa tagcggaaaa atagataaac
    30241 aaaccgttac ataaaagacc aaagtatgat taaatcgttt ccggtaagct ttatagataa
    30301 aaactagcag agactgtccg acaacgtatg ccaatagtga cttcaattga ttaaaagtac
    30361 taccagtcaa catcattaca ataaaactta gactaaaaat aatctcatat aaggcaaaac
    30421 gcttcttaaa gaataagccg atgtaaattg gtaggacagc taagataagg tagaagaaat
    30481 attgtggatt cccataaaca tccaagtgag gaagtttttc taaaaatgcc tgtatcactt
    30541 gtttacctcg ctcataagac ctttgatatc aattttccca tttggtgtca aaggtaaatc
    30601 ctctcgatag ataaatttag atggcatcat gtaatccatc ataatgtcct ttaagtcttc
    30661 cttaattgct tttgtcaaat ccaaatcacg ttcaaaatca tctcttacac cttcttttaa
    30721 gacaatatag gctaataagt tttgaacttt atgatccttg ttataacgtg gcactgctac
    30781 tgctgatttt acatactgcg atttgtttaa gttttgagaa acatcttcta attcaatacg
    30841 atagccgtta aatttaattt ggaaatccat acgcccaccg taaagcagaa gaccttcatc
    30901 ggtcatactt cctaagtcac cggtatggta agcaggtaga ccttcgaatt ggaaaaatgc
    30961 ctcagctgtc ttttctggat tgttaagata gccttttgat actgctggtc cagcaataat
    31021 gatttcacct tgctctccgt ttggtaattt atgaccttct tcatcaatca catacgttgg
    31081 tgagtcatct ttagtgtaac caattggaag gcgtttgcat gtttctaaca tttcatcagt
    31141 aattgctact gcggatagag caactgttgc ttctgttggc ccatatgcat taacgatacg
    31201 agcttttgga aaacgttgac gaagtttttg tgcagtcttg acagttaact cttccccatc
    31261 aaaataaaaa tgtgttaact gtggcaaggt ctctgaattg aaatcgttag atagtagcgc
    31321 catatcagca aatgatggtg tcgaagtcca aacctgtatt ggcaattcat taatggtagc
    31381 gaatagtttt ttgaaatcat taactactgt ttttggtagg gcaaacaggg tgcctcccat
    31441 agctagtgtt ggtgcccaat acataactga taagtcaaat gaatatggcg gttgagccaa
    31501 catttgcggt ctttcaggaa ctgaaaactc atcatcagaa atcatccaat ttgtaaagct
    31561 caataaattg tcatgtgaaa tttgcacacc ttttggtaaa ccagtagtcc ctgaagtgaa
    31621 aataatatag taattatcat cacctttaac agaatgtgtt acctcataag gagtcttttc
    31681 ttcaaaaata gctgaaactt gagaaacgtc taggattggg acattatcaa cttcaagagg
    31741 aaattcacca attgaaatga taaggcttgg ttgagcaact gtcataatag cctgtattct
    31801 atccaaagca gagtgttggt caaccggtat ataagcatgc cctgactttg ttaaagcaac
    31861 aaatgtcgcc aacatttcat attcttgacc accgaatact aagacaggtg atttttcaac
    31921 aaggcctagg ctatcaatat gagcagctag agagtcagag tctactttaa gttgtccata
    31981 agtatggact tcccctaaaa tatcatacac tggaaaatca gcttgtgtct cagcaaaatg
    32041 ctcaattgtt ttaatcatat catgtatcat ttgtaccatc tcctagaatt cattataaat
    32101 aaaactgcct tgtccgcgac caaggtatgc aaaaaagtaa attagtaaca ataaaatacc
    32161 gaagtataat aacgttctac caataaaaga taaatatggt ttcatcattc ttcctttaat
    32221 taatcatggt attggtccaa aatccatcaa ttggtaactg gacttcaaga cgtaattggt
    32281 ctttttcttg atatgctcta attctcccct tatggcgttc cacgattgct ttagcaatag
    32341 ataagcctag accaaaactg agagaatctg catgatcatc tttagcttga taaaaacgct
    32401 caaagagatt atccataacc tctttggaat agatagcact tggatttgat attgtcagaa
    32461 tagcttttct tcgagcttgt ttaagactaa actggattgc tgattttgac aaagaatgcc
    32521 taatagcatt atccaaaaga attattaaaa tttgcttaat ctgactctct tctccaacaa
    32581 tccagacatc atcttcaatc gtatcattta aacacttttc ttcttcatat gccaaacttt
    32641 catatgttag aagtacttct tgacataaga cagataagtt taactttttg aaatttacct
    32701 tggagcgttt ttcatcaagt ctagacaaat taagcaaatc ttcaattaaa aactccattc
    32761 gcttagtctc cgaaacaaca tgatccagat aacgattccc tggaatttgc tgttccagaa
    32821 cctgaacgtt ggctcgtata gctgcaatag gggtcttcag ctcatgactc gcatctgaaa
    32881 cgaactgttt ttcgcgagtc atttctgtct ctacaggtcc tacaataaat cgagaaagat
    32941 aaagacttac agctgctaat aaacaaaatc caaaaatagt aaaaacaaga attagaaaac
    33001 gccttgcata agggacttca taactagcca aatccataat agctatggtg taggttttat
    33061 ctttatattc ttttatggaa tatataaaag acttccattt tcgtgacttc tttccttgct
    33121 caaggagctt caaactagat ttttcgataa catcatttgt caaatcagta ttattgcttt
    33181 gaatcttaac catcttttgg ttatctatag tcaccaaagc aatcggttca tcgcctggta
    33241 ttcctaaata atcatttttg gcaactaatt taacaatcct atatgcatca tattcatccc
    33301 aatattgatt gtagcgatta ctaactgcaa atagaaagag aacaacgaca ataaggattg
    33361 acatggtcaa aaagacaaat tttttgcgta aatcactaaa cattatgtcc tctcctccaa
    33421 ggaatatcca aagcccttag tggttattat ttcaatgtct gcttttaaaa agcgcaattt
    33481 ttttcgcaaa aaagaaataa atacttctaa ctggttatat tctgaattgt ctgaaggtcc
    33541 ccaaacctta gaaatcaact gatttttagg aataatctgc tttgcatttc gcataaaaac
    33601 ttccatcaag agaaattctt tattggataa ttttacacta ctttctttag attctagctc
    33661 atgtgaatct gtgttaagcc ttatatttcc aagccgcaat tgatttgctc tgattagaga
    33721 agattgtctg gttctcagcc taattctagc tagcaattca tctgcttcaa agggttttgt
    33781 gatataatca tctgccccta aatctaaacc attaacttta tcataagtct gtgattttgc
    33841 agttaaaaag attattgggg tttctaaacc agcttccctc actcttttta aaacagataa
    33901 tcctgagcgt ttgggtaaca taatatccaa caggactaaa tcatagagac ctgtcaaaat
    33961 gtattctaac ccatcatctc catcaaatgc taaatcaaca gaataacctt tatctgccaa
    34021 taaagcctga atagcctctg ctatactttt ttcatcctca actacaagaa gtctcatttt
    34081 ctagtcacct taccttatat attatagtat aaactagcaa aattgaaata accttgaaaa
    34141 aacgatatta ataaaagtat ttcctaagtc actaaactac ttcaataata caagcgtcta
    34201 aaaaataaaa tatcactcgc tataaagata tttctcaata agaaatctcc tcatattcta
    34261 acaaaaatag gggctatttg tctacgaatt tccgcattat aaactcactt ttcattaact
    34321 ttatctataa tatactcaaa ctactctcac aaaaaccttt atcgttatga aggtaccaaa
    34381 aaagccacca gactcgagta cctgttggct tgatattatt ttgctatcta tattaagctg
    34441 ataacacttt acgtccttta cgacggcggc tagcaagcac gcgacgtccg tttttagttg
    34501 acatacggtg acggaatccg tgtttacgtt ggcgacggat tttacttggt tgataagtac
    34561 gtttcacgtt gaatacctcc tgatagattt tcgtattcgt ttagccggct attttgatca
    34621 gttactaaac ataccatact attttataac aattttaggc ctcttgtcaa gaaatacttt
    34681 caagttcatt tgtctttttt cagtaaatag ttctatttgt tagcaagaaa tgcttctcta
    34741 ttttctccaa ctaaaagaaa agatgtgcca tcaaaacaat aataggtaat gaaataattg
    34801 ttctctctat aaaaatgata aataattcta gaatgttgat agggatcttg gaacctaata
    34861 tcaccgcacc agtctcagac atataaatta attgcacaat agaaactgtt gcgacaataa
    34921 accttgtcat ctcactgtga atttcagccg ccattatcga aggaacaacc atatctgaaa
    34981 aaccaacaat catggtttga ctcgcaatat ctgcttctgg aatccctaaa agttcaagga
    35041 acggaaggaa tggtgtacct aaaattttaa aaataggtgt tgatactgaa ataatcaaag
    35101 ctattgttcc gacagtcata attgttggca taacgccaaa ccataaacta agtactgtct
    35161 tagaggcatt attgacaaaa gtcttcatgt ttccatttgc ttttgattta tcgatggcaa
    35221 gcttaagtcc ccactctatc ttgttataac catccggaat tacctcagat gtttctttct
    35281 ttggtttagt gtaataacta tttgattgac gagataaggg agggatataa gggataatga
    35341 atgcacagac aacactgcta atgatgactg ttagataaaa atggccaaaa taatcagtta
    35401 agccaacatt ctgcaaaaca acaagacaaa aagtgattga tactgctgaa aaagtagtgg
    35461 aaatcgttgc agcttctcta ctggtataat aaccctcaac atactgctta tcggtcaaag
    35521 caattccaat agtaccatca cctacaaatg aggctacaca attaactgct gaccgaccag
    35581 gtagctgaaa gaatggacgc ataatgggag ttaggaagac accaacaaac tccagtagac
    35641 caaattctgt caaaaatgga agaataaaac ccgcagcaag gaaaatggta aacaaaccac
    35701 caatcaaatc aaaaaggata aggccgcctg tctctttccc acataccatt tcaggaccaa
    35761 tatgtaaaac cgtcatcaga ccaagtgcca aaccgatcaa acgaataatc agccagaaaa
    35821 tggtaatgtc agaaatttct tttagcaagt catttttttc aataaagcta ggtctaaaca
    35881 gacgataaca aagtgctaac acacatgaaa taaaaatgat tagtaaaata atataatgta
    35941 taggaaaaac agcgttaatc cattgattaa ttgtcttaga gattacagag acagctactg
    36001 ttgtcatacc attatattta aatggagtca ttaataataa tactccaaat aacgacggaa
    36061 taataaacca caagatatct ttaatagata tactttcttt tttcatacaa tcctcctgga
    36121 taatatggaa ttgtttctaa atatactcat aacttccatt gtaaaatttt ataataaaaa
    36181 aaacaacctt gaattaaaga aaattttact tagtaatact ttagtaattt ctcaaagtaa
    36241 gttccgtctg ccctccaaaa ccggaagtta gtgtgaaaag gatttttatg gtggaattaa
    36301 gtct
//
LOCUS       DATROQ010000012        33095 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.012, whole genome shotgun sequence.
ACCESSION   DATROQ010000012 DATROQ010000000
VERSION     DATROQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33095
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.012"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..1254)
                     /locus_tag="VBC94_000533"
     CDS             complement(<1..1254)
                     /locus_tag="VBC94_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO6978828.1"
                     /translation="MTEFNDDQHSNHDQKSFKEQILAELEEANRLRKLREEELYQKEQ
                     EAKEAARRTAQLMADYEAQRLKDEQEARAKALETKQRLEEQEKARIEAKLLAEAAREE
                     ERRQAEQALASQEEQVINQGMEPSRELDSGSKSSEFRTTENVPDIDLKADKTDVATAV
                     PNQETEEIFLVRATDIPTEGENVKLGETSELEPVAKEPIRVEDLSKEEEGIALSAKNK
                     HNKRERRQKADNVAKRIARILISIIILVLLLTAFVGYRFVDSAIKPVDSNSNKFVQVE
                     IPIGSGNKLIGQILEKAGVIKSATVFNYYSKFKNYSNFQSGYYNLKKSMTLDQIAAEL
                     EKGGTAEPTKPALGKILITEGYTIKQIAKAIESNKIDTKTTSTPYKADDFLKLVQDET
                     FIKKMVAKYPNLLGSLPDKSKAIYQL"
     gene            complement(1341..1844)
                     /locus_tag="VBC94_000534"
     CDS             complement(1341..1844)
                     /locus_tag="VBC94_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6978829.1"
                     /translation="MIIRNGCLEDLQQVISIEQINFSEAEAASKKAMQERLTIMTDTF
                     LVAEINGRLAGYIEGPVIKGRYLTDDLFHKVSEFPVRVGGFIGITSLSIHPDFKGQGI
                     GTALLAAMKDLVVSQERDGISLTCHDDLISFYEMNGFKDEGESDSQHGGSLWYNMIWN
                     NPTRSIC"
     gene            complement(1930..3261)
                     /locus_tag="VBC94_000535"
     CDS             complement(1930..3261)
                     /locus_tag="VBC94_000535"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEO6978830.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHNVQGSDVDKYYFTQRGL
                     EQAGVTILPFSPNNISEDLEIIAGNAFRPDNNEELAYVIEKGYHFKRYHEFLGDFMRQ
                     FTSLGVAGAHGKTSTTGLLAHVLKNITDTSFLIGDGTGRGSANANYFVFEADEYERHF
                     MPYHPEYSIITNIDFDHPDYFTGLEDVFNAFNDYAKQVQKGLFIYGEDPKLHEITSEA
                     PIYYYGFEDSNDFIAKDITRTVNGSDFKVFYNQEEIGQFHVPAYGKHNILNATAVIAN
                     LYIMGIDMALVAEHLKTFSGVKRRFTEKIIDDTVIIDDFAHHPTEIIATLDAARQKYP
                     SKEIVAIFQPHTFTRTIALLDEFAHALSQADSVYLAQIYGSAREVDNGEVKVEDLAAK
                     IVKHSDLVTVENVSPLLNHDNAVYVFMGAGDIQLYERSFEELLANLTKNTQ"
     gene            complement(3271..3888)
                     /locus_tag="VBC94_000536"
     CDS             complement(3271..3888)
                     /locus_tag="VBC94_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978831.1"
                     /translation="MNRGSRRFPLIADDEPVMSPLVKMNLYDNEDLINNIRGFYQEKT
                     YQSMVKSNYEHEEISHPKVIENDPVPPQSFVKKATELSKSRQEAKRSVREKRQAYYAK
                     QEFKAPSKEAFQQQLKATVPKKQTQRKVTELSHLSDRLQQESYILAEIPIIFQEPDNT
                     PNPKTKKNNFDFLKRSQVYNKQDNQFHKERAKAQELNLTRFKDIN"
     gene            complement(4053..7151)
                     /locus_tag="VBC94_000537"
     CDS             complement(4053..7151)
                     /locus_tag="VBC94_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6978832.1"
                     /translation="MSRMIPGRIRNQGIELYEQGLVSLISQEGNLLKAKVGDCQIEYS
                     LVTEETKCSCDFFARKGYCQHLAALEHFLKNDPEGKAILSKVQVQQESQQETKKKTSF
                     GSVFLDSLIINEDDTIKYQLSAQGEQNPYANDIWWTLKIRRLPDDRSYVIRDIKAFLN
                     TVRKEAYYQIGKQYFETLSLIQFDETSQELIEFLWRLIPSHSSKIDLEFILPNQGRHL
                     SLTRGFFEEGVTLMNALENFSFESDFHQFNHLYFKELEGEDHLYQFKVIVHRQSIELE
                     IKEKDLKPLFANSYLFYRDTFYHLNLKQEKMVTAIRSLPIEGNLAKHIHFDLDDQDKL
                     AAHLLDFKEIGLVDAPRSFSIHDFKVNFEFDINSQNEILLQMVFDYGNDLTVHNRQEL
                     EQLTFASHFKHEEKVFKLLEKYGFAPHFSTSHPAYSAQELYDFYTYMLPQFKKMGTVS
                     LSAKLESYRLIERPQIDIEAKGSLLDISFDFSDLLENDVDQALVALFDNNPYFVNKSG
                     QLVIFDEETKKVSATLQGLRARRAKNGHIELDNIAAFQLSELFANQDNVSFSQHFYQL
                     IEDLRHPEKFKIPGLSVSASLRDYQLTGVRWLSMLDHYGFAGILADDMGLGKTLQTIS
                     FLSTKLTRDSRVLILSPSSLIYNWQDEFHKFAPDVDVAVAYGSKIRRDEIIAERHQVI
                     ITSYSSFRQDFETYSEGNYDYLILDEAQVMKNAQTKIAHSLRSFEVKNCFALSGTPIE
                     NKLLEIWSIFQIILPGLLPGKKEFLKLNPKQVARYIKPFVMRRRKEEVLPELPDLIEM
                     NYPNEMTDSQKVIYLAQLRQIQESIQHSSDADLNRRKIEILSGITRLRQICDTPRLFM
                     DYDGESGKLESLRQLLTQIKENGHRALIFSQFRGMLDIAEREMVAMGLTTYKITGSTP
                     ANERHEMTRAFNAGSKDAFLISLKAGGVGLNLTGADTVVLIDLWWNPAVEMQAISRAH
                     RLGQKENVEVYRLITRGTIEEKILEMQETKKHLVTTVLDGNETHASMSVDDIREILGV
                     SK"
     gene            complement(7306..8616)
                     /gene="der"
                     /locus_tag="VBC94_000538"
     CDS             complement(7306..8616)
                     /gene="der"
                     /locus_tag="VBC94_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEO6978833.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYT
                     TGEWLNRKFSLIDTGGIDDVDAPFMEQIKHQADIAMTEADVIVFVVSGKEGVTDADEY
                     VSRILYKTNKPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPLSSVHGIGTGDILDAIV
                     ENLPVEEENENPDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTNFV
                     DSQGQEYTMIDTAGMRKSGKVYENTEKYSVMRSMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHETGKGIIIVVNKWDTIEKDNHTVSQWEADIRDNFQFLSYAPIIFVSAETKQ
                     RLHKLPDMIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYLRFLENQIREAFVFEGTPINLIARKRK"
     gene            complement(8664..9566)
                     /gene="dnaI"
                     /locus_tag="VBC94_000539"
     CDS             complement(8664..9566)
                     /gene="dnaI"
                     /locus_tag="VBC94_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEO6978834.1"
                     /translation="MKSVGQALENQGRVPRNTNDELIQMILADAQVAEFIKTHQLSQR
                     EINISMSKFNQFLIERQKFKNKDSQYIAKGYEPILVMNEGYADVSYLETRELIEAQKK
                     QAISDRINLVNLPKSYRNIRMTDFDINNESRMKAMSQLLDFVETYPSYNHKGLYLYGD
                     MGVGKSYLMAAMARELSERKGVSTTLLHFPSFAIDVKNAISSGTVKDEIDAVKSVPIL
                     ILDDIGAEQATSWVRDEILQVILQHRMLEELPTFFTSNYSFNDLERKWANIKGSDETW
                     QAKRVMERVRYLAIEFHLEGPNRR"
     gene            complement(9563..10738)
                     /locus_tag="VBC94_000540"
     CDS             complement(9563..10738)
                     /locus_tag="VBC94_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEO6978835.1"
                     /translation="MSPIDEFTYIKQNKIVYDSNSLIQLYFPIMGSDAMALYDYFVHF
                     FDDGIRRHKFSEVLNHLQYGMPRFQDALVMLTALDLLTVYQATGTYLVKLNQAMSNEL
                     FLSNPIYRRLLEKRIGEVAVAELDMKIPKNARDISKKFTDVFSDLGQPKQEVNRSKNV
                     FDLESFKRLMMRDGLRFNNEKDDVLGIYSVSELYHLNWYDTYQLAKQTAINGMIAPQR
                     MKLQQNEGQHIKDNQSFTNNEKVILRESKNDSALVFLEKIKRSRKAVTTSGEKTLLED
                     LAKMNFLDEVINVMVLYTLNKTKSANLNKAYIMKVANDFAFQNVITAEDAVLKIRDFS
                     DQKVRTKTETKKKQSNVPEWSNPDYKDEVSPEKEIELEQFKTDALKRLERLGKDGES"
     gene            complement(10738..11217)
                     /gene="nrdR"
                     /locus_tag="VBC94_000541"
     CDS             complement(10738..11217)
                     /gene="nrdR"
                     /locus_tag="VBC94_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEO6978836.1"
                     /translation="MRCPKCGYNKSSVVDSRQAEEGTTIRRRRECEKCGNRFTTFERL
                     EELPLLVIKKDGTREQFSRDKILNGIIQSAQKRPVSSEDIENCILRIERKIRSEYEDE
                     VSSITIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITKRVRSKKSGSV"
     gene            complement(11366..12871)
                     /locus_tag="VBC94_000542"
     CDS             complement(11366..12871)
                     /locus_tag="VBC94_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEO6978837.1"
                     /translation="MKNKKDQFIGVKQPLSKKLSQLVFILFFSLFTVFSVLVYTSATR
                     YVLHREKINVGRSLEKTRVRLSQANSSLTSDDILEILYNQVFADDIYPHKRQNGIVRT
                     GESIDSILYVNQEMTLYDVNRKPVFSTLRTGMPTIGKSMGKVIISKVADMEGFVGTKA
                     IYSQKTGQLLGYVQIFYNLGRYYSMRQNIIVFLIMMEVLGTVLALVVINSATKRIVRP
                     VKNLHDLMHQISENPSNLEIRSKVRSEDEIGELSRIFDGMLDQLEDYTRRQSQFISDV
                     SHELRTPVAVVKGHIGLLQRWGKDDPEILEESLAAAYHEADRMSLMINDMLNMIRVQG
                     SLELHQDEVTDLSSSISVVIENFRILREDFQFIFENNISDIVWGKIYKIHFEQALMIL
                     IDNAIKYSPSYKEVSVVLSVDNDFATVVVKDKGEGISDEDIEFIFDRFYRTDKSRNRE
                     STQAGLGIGLSVFKQIMDAYHLKVDIKSELNQGTEFIVRIPIKKFEETKEI"
     gene            complement(12861..13550)
                     /locus_tag="VBC94_000543"
     CDS             complement(12861..13550)
                     /locus_tag="VBC94_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6978838.1"
                     /translation="MGKKILIIEDEKNLARFVSLELLHEGYDVVVETNGREGLDTALE
                     KDFDLILLDLMLPEMDGFEITRRLQAEKTTYIMMMTARDSVMDIVAGLDRGADDYIVK
                     PFAIEELLARVRAIFRRQEIETKTKEKGDSGSFRDLSLNTHNRSAMRGDEEISLTKRE
                     FDLLNVLMTNMNRVMTREELLEHVWKYDVAAETNVVDVYIRYLRGKIDIPGRESYIQT
                     VRGMGYVIREK"
     gene            complement(13785..14318)
                     /locus_tag="VBC94_000544"
     CDS             complement(13785..14318)
                     /locus_tag="VBC94_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEO6978839.1"
                     /translation="MLLTEIKKSPEGLYFDKKIDIKESLMERHSEIMDISDIQVSGHV
                     VYEDGLYLLDYNMAYDITLPSSRSMKPVVLSEKQTINEVFIEAENVSTKKELVDQELV
                     LILEEDDINLEESVIDNILLNIPLRVLAADEVGVEADLSGKNWSLMTEKQYEEKQAKE
                     KEKSNPFAALEGMFDSD"
     gene            complement(14423..15313)
                     /gene="htpX"
                     /locus_tag="VBC94_000545"
     CDS             complement(14423..15313)
                     /gene="htpX"
                     /locus_tag="VBC94_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEO6978840.1"
                     /translation="MLYQQIASNKRKTVVLLIVFFCLLAAIGAAVGYLVLGSYQFGLV
                     LALIIGVIYAVSMIFQSTNVVMSMNNAREVTEDEAPNYFHIVEDMAMIAQIPMPRVFI
                     VEDDSLNAFATGSKPENAAVAATTGLLAVMNREELEGVIGHEVSHIRNYDIRISTIAV
                     ALASAVTLISSIGSRMLFYGGGRRRDDDREDGGNILVLIFSILSLILAPLAASLVQLA
                     ISRQREYLADASSVELTRNPQGMISALEKLDRSEPMGHPVDDASAALYINDPTKKEGL
                     KSLFYTHPPIADRIERLRHM"
     gene            complement(15334..15888)
                     /locus_tag="VBC94_000546"
     CDS             complement(15334..15888)
                     /locus_tag="VBC94_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEO6978841.1"
                     /translation="MGTMILIAIIALFVIWLIVAYNSLVRSRMHTKESWSQIDVQLKR
                     RNDLIPNLIETVKGYAAYEGKTLEKIAELRAQVAKANTPAEAMTASNELTRQLSSILA
                     VAENYPDLKANNSFVKLQEELTNTENKISYSRQLYNTTTSNYNVKLETFPSNIVGKLF
                     GFKPSQFLETPEEEKEVPKVSFDF"
     gene            16016..16729
                     /gene="rsmG"
                     /locus_tag="VBC94_000547"
     CDS             16016..16729
                     /gene="rsmG"
                     /locus_tag="VBC94_000547"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEO6978842.1"
                     /translation="MTPQAFYQVLIEHGITLTDKQKKQFETYFRLLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGYIDNSPLSILDIGAGAGFPSIPMKILYPEIDITIIDS
                     LNKRINFLNILANELELSGVHFFHGRAEDFGQDKVFRAKFDIVTARAVARMQVLAELT
                     IPFLKVNGRLIALKAAAAEEELISAEKALKTLFSQVTVNKNYKLPNGDDRNITIVSKK
                     KETPNKYPRKAGTPNKKPL"
     gene            16744..18123
                     /locus_tag="VBC94_000548"
     CDS             16744..18123
                     /locus_tag="VBC94_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO6978843.1"
                     /translation="MKHFFDYKTMSVARKLSISFIAVILLGSILLSLPIFQYANAPKT
                     HYIDHLFTTVSMVCVTGLSVFPISKVYNGWGQIVAILLMQTGGLGLVTLMSLSYYTLR
                     RKMSLNDQTLLQSAITYNSSTDLKKYLYMIFKVTLTLEVLAASILAIDFIPRFGLGHG
                     IFNSIFLAVSAFCNAGFDNLGATSLAQFKLNPLVNIIVCFLIISGGLGFAVWKDLIEA
                     TIQTSHKEPKLIKTFPKRLSNHSKLVLKTTTIILLTGTLLSWLLEFGNFRTIANLSLP
                     KQLMVSFFQTVTMRTAGFSTIDYTQTDFATNLVYIIQMLIGGAPGGTAGGFKVTVIAI
                     LLLLFKAELSGQSQVTFHYRTIPSSIIKQTLSILTFFFIILISGYLLLLELNPHIDPF
                     SLFFEASSALATVGVTMNTTNQLTLGGRIVIMFLMFIGRVGPITVLLSILQKKEKEIH
                     YAETEIILG"
     gene            18136..18807
                     /locus_tag="VBC94_000549"
     CDS             18136..18807
                     /locus_tag="VBC94_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEO6978844.1"
                     /translation="MKTKIIGVLGLGIFGQTLAQELSNFEQDVIAIDSNPENVQAVAE
                     VVTKAAIGDITDLAFLKHIGISDCDTVIIATGNSLESSVLAVMHCKKLGVPQVIAKAR
                     NLVYEEVLYEIGADLVISPERESGQNVAANLMRNKITDVFQIESDISVIEFKIPKSWV
                     GKTVEQLNIRHKFDLNLIGIRKAKNKPVDTEVPINTPLEEGIILVAIANSDAFQRYDY
                     LGYFN"
     gene            complement(18842..19672)
                     /locus_tag="VBC94_000550"
     CDS             complement(18842..19672)
                     /locus_tag="VBC94_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO6978845.1"
                     /translation="MAKQLIGYQEGYGFLYRLSGASKLLFFILVSVACMTTYDTRFLI
                     VVSLGSLVLFRLAKIKWQQVSFVMTLVVVFAVLNIIMVYLFAPHYGDKIYGSSSLLLK
                     GIGLYDVTSQELFYLFNLILKYFCTVPLALLFLMTTNPSQFASSLNQLGLSYKIAYAV
                     SLTLRYIPDVQEEFYTIRRAQEARGIELSKKSNLVARIKGNLQIVTPLIFSSLERIDT
                     VATAMELRRFGKNKRRTWYSKQSLEKSDIVLIILALASLFVSLYLIHLNHGRFYNPWQ
                     "
     gene            complement(19665..21341)
                     /locus_tag="VBC94_000551"
     CDS             complement(19665..21341)
                     /locus_tag="VBC94_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6978846.1"
                     /translation="MKDFIEWKDFTFQYDVQSEPTLKGINLSIPKGEKVLILGPSGSG
                     KSTLGHCLNGIIPNTHKGQYSGIFTINHKNAFDLSIYDKSHLVSTVLQDPDGQFIGLT
                     VAEDIAFALENDVVAQEEMASIVEMWAKRLEIAPLLSKRPQDLSGGQKQRVSLAGVLV
                     DDSPILLFDEPLANLDPQSGQDIMALVDRIHQEQDATTIIIEHRLEDVLYERVDRVVL
                     FSDGQIIYNGEPDQLLKTNFLSEYGIREPLYISALKNLGYDFEKQNTMTSIDDFDFSE
                     LLIPKMRALDLDKHTDKLLSVQHLSVSYDLENNTLDDVSFDLYKGQRLAIVGKNGAGK
                     STLAKALCQFIPNNATLIYNNEDVSQDSIKERAERIGYVLQNPNQMISQAMVFDEVAL
                     GLRLRGFSDNDIESRVYDILKVCGLYQFRNWPISALSFGQKKRVTIASILILNPEVII
                     LDEPTAGQDMKHYTEMMSFLDKLSCDGHTIVMITHDMQLMLEYTDRAIVIDNGLIVAD
                     DHPINILSNTELLKKTHLKKTSLFALADRLGISPQKLTQWYIDNQGGKNG"
     gene            complement(21358..21906)
                     /locus_tag="VBC94_000552"
     CDS             complement(21358..21906)
                     /locus_tag="VBC94_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEO6978847.1"
                     /translation="MNTNTIKKVVATGIGAALFIIIGMLVNIPTPIPNTNIQLQYAVL
                     ALFAVIYGPGVGFFTGFIGHALKDSIQYGSPWWTWVLVSGLLGLMIGFFAKKLAIQLS
                     GMIKKDLLLFNVVQVIANLIGWSVVAPYGDIFFYSEPASKVFAQGFLSSLVNSITIGV
                     GGTLLLLAYAKSRPQKGSLSKD"
     gene            complement(22075..23283)
                     /gene="sstT"
                     /locus_tag="VBC94_000553"
     CDS             complement(22075..23283)
                     /gene="sstT"
                     /locus_tag="VBC94_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEO6978848.1"
                     /translation="MKRFLKAWRKTSLIKKITIGVVIGLFLGILVPKASAIGLLGQLF
                     VGGLKAIAPLLVFTLVISALSQHREGGKTNMSTIIGLYITATFAAALIAVVVNYIFPL
                     TLILKTPAKTDLLPPKGISEVFQSLLLKIVDNPIHAITEANYMSILFWAVIFGLAMRS
                     SNQRTKDLMQTFADATSQVVKWIINLAPIGIMGLVFTSISENGIGILGDYGLLILVLV
                     GTMLFVALVVNPIIAFVMMRKNPYPLVLRCLKDSGITAFFTRSSAANIPVNMRLCEDL
                     GLDKDTYSVSIPLGAAINMAGAAITINILTLAAVNTLGITVDFPTAFLLSVVAAVSAC
                     GASGVTGGSLLLIPVACSLFGISNDVAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     VAEKSVFGKK"
     gene            23459..24826
                     /gene="brnQ"
                     /locus_tag="VBC94_000554"
     CDS             23459..24826
                     /gene="brnQ"
                     /locus_tag="VBC94_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO6978849.1"
                     /translation="MKLKNTYIIIGLMLFALFFGAGNLIYPAYLGIYSGSNWAWAIIG
                     FCLTGVTLPLLGVAAVTYSGATDVESFTKPVSKTYAIFFAVALYLSIGPFFAIPRTGA
                     TSFSVGIQPILGDSSLVKIIYGLIFFGISYFLAIRPSQIVDRIGKYLTPFLLTVISIL
                     IIASLIHPAAIVGTPHNASTNVSDAFKDLPFLAGLIQGYGTMDALASIAFAIIVIQAS
                     KQYGAITKKEITSMTLKSGAIATFLLAFIYIFVGRIGATSQSLFKFANGSFLLHNTPI
                     DGGHVLSQSANFYLGIVGQAILGTAIFLACLTTATGLITACAEYFHKLLPKISHITWA
                     TIFTLIAITFYFGGLSEIIRWSLPVLYLLYPLTIVLIFLVFFDQKFESSRIVYQTSIA
                     ATAVAALYDALSKLGEMTGLFTIPSALTTFFTKVVPLGEYSMGWISFAICGVLVGLIL
                     KKVKA"
     gene            24940..25995
                     /locus_tag="VBC94_000555"
     CDS             24940..25995
                     /locus_tag="VBC94_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO6978850.1"
                     /translation="MRAATYLSANELSLTDKAKPQVIKPTDAVVRLVKTTICGTDLHI
                     LGGDVPACKPGTTLGHEGIGIVEEVGSAVNHFKVGDKVIISCVTACNTCYYCKHQLPS
                     HCEDGGWILGHLIEGTQAEYVHIPHADGSLYHAPEGVCDDALVMLSDILPTSYEIGVL
                     PSHIKPGDTVCIVGAGPIGLSALLTAQFYSPAKIIMVDLSQKRLEASKKFGATHTILS
                     TSTQEVKEEIDKITKGRGVDVVLECVGYPATFDICQNVVSIGGHIANVGVHGKPVEFN
                     LQDLWIKNITLNTGLVNANTTEMLLEVLETGKIDATQLVTHHFKLSEIEEAYKVFKAA
                     EENNTLKVIIENDITPA"
     gene            complement(26044..26736)
                     /locus_tag="VBC94_000556"
     CDS             complement(26044..26736)
                     /locus_tag="VBC94_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000574038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6978851.1"
                     /translation="MIEWIQTHLPNVYQMGWEGAYGWQTAIVQTLYMTFWSFLIGGLM
                     GLLGGLFLVLTSPRGVIANKLVFGVLDKVVSVFRALPFIILLALIAPVTRVIVGTTLG
                     SPAALVPLSLAVFPFFARQVQVVLAELDGGVIEAAQASGGTLWDIIVVYLREGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGSVAITKGYNYSRDDITLVATILILLLIFFIQF
                     LGDFLTRRLSHK"
     gene            complement(26733..27803)
                     /locus_tag="VBC94_000557"
     CDS             complement(26733..27803)
                     /locus_tag="VBC94_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEO6978852.1"
                     /translation="MSKEPIIKLDNIDVTFHQKKREINAVKDVTIHINQGDIYGIVGY
                     SGAGKSTLVRVINLLQEPSAGKITIDDQVIYDNKVTLTSTQLREQRREIGMIFQHFNL
                     MSQLTAEQNVAFALKHSGLSKEAKAAKVAKLLELVGLSDRAQNYPSQLSGGQKQRVAI
                     ARALANDPKILISDESTSALDPKTTKQILALLQDLNKKLGLTIVLITHEMQIVKDIAN
                     RVAVMQNGKLIEEGSVLDIFSHPRESLTQDFIKIATGIDEAMLKIEQQEVVKNLPVGS
                     KLVQLKYAGHSTDEPLLNQIYKEFEVTANILYGNIEILDGIPVGEMVVILSGDEEKLR
                     QACQAITDSQVQLTLLKEGGKA"
     gene            complement(27796..29172)
                     /locus_tag="VBC94_000558"
     CDS             complement(27796..29172)
                     /locus_tag="VBC94_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEO6978853.1"
                     /translation="MTLTNEENQIQKFLSDTIVQDDINILRDLIAIKSIFAQKVGLND
                     LSSYLGEVFRKAGAEVIIDDSYSAPFIVANFKSSKVDAKRIIFYNHYDTVPADEVEQW
                     TEDPFTLSLRYGKMYGRGVDDDKGHITARLSAVKKYLSRHKGELPLDITFIVEGAEES
                     ASVGLDYYLEKYQEQLQGADLIVWEDGPKNPKGQLEIAGGNKGIVTFDLSVSSADVDI
                     HSSFGGVVDSSTWYLIQALNTLRDNKGHILVEGIYDKVIPPTKRELELVEKYSYRSAK
                     ALEGAYQLVLPSLADSHKTFLRKLYFEPSIAIEGITSGYQGEGVKTILPAYAKCKAEV
                     RLVPGLTPKGVLDSIQNHLKENGFKDIELTYTLGEMSYRSDMSAPSILKVVDLAEQFY
                     PEGISLLPTSPGTGPMYLVHQALRAPIAAIGIGHANSRDHGVDENVSIADYYTHIELV
                     EALIESYE"
     gene            complement(29306..30130)
                     /locus_tag="VBC94_000559"
     CDS             complement(29306..30130)
                     /locus_tag="VBC94_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO6978854.1"
                     /translation="MKLKHIVLGLALTTLLGVTFSNQEVSASSTSSKVVKVGVMTFSD
                     TEKARWDKIEKLVGDKAKIKFTEFTDYTQPNQATANKDVDINAFQHYNFLENWNKENK
                     KNLIPLEKTYLAPIRIYSEKVKSLKKLKKGATIAIPNDATNGSRALYVLQSAGLIKLN
                     VSGKKVATVANITSNKKDINIQELDASQTPRALKDVDAAIINNTYIEQANLKPSDAIF
                     VEKSDKNSKQWINIIAGRKNWKKQKNAKAIQAILDAYHTDEVKKVIKDTSADIPQW"
     gene            complement(30271..31104)
                     /locus_tag="VBC94_000560"
     CDS             complement(30271..31104)
                     /locus_tag="VBC94_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6978855.1"
                     /translation="MTIKKVLSVTGIILVTVASLAACSSKSHTTKTGKKEVNFATVGT
                     TAPFSYVKDGKLTGFDIEVAKAVFKGSDNYKVTFKKTEWSSVFTGIDSGKFQMGGNNI
                     SYSSERSQKYLFSYPIGSTPSVLAVPKNSNIKAYNDISGHKTQVVQGTTTAKQLENFN
                     KEHQKNPVTLKYTNENITQILTNLSDGKADFKLFDGPTVNAIIKNQGLTNLKTIPLTM
                     RDQPYIYFIFGQDQKDLQKYVNNRLKQLRKDGTLSKIAKEYLGGDYVPNEKDLVTPKE
                     K"
     gene            complement(31206..31895)
                     /locus_tag="VBC94_000561"
     CDS             complement(31206..31895)
                     /locus_tag="VBC94_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEO6978856.1"
                     /translation="MLTKPIIGITGNEREMSDIPGYYYDSVSRHISEGVKNAGGLPVI
                     LPISEAESAKAYVEMIDKLIISGGQNVLPSYYGEEKIIESDDYSLARDIFEFALVEEA
                     LKQNKPIFAICRGMQLVNVALGGTLNQSIDNHYQEPYIGFAHYLNVEKGSFLEGFISG
                     DFKINSLHRQSVKLLAEGLIVSARDPRDGTVEAYESRTEQCIIGVQWHPELMLHQIEN
                     QTLFGYFVNET"
     gene            complement(32050..32766)
                     /locus_tag="VBC94_000562"
     CDS             complement(32050..32766)
                     /locus_tag="VBC94_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6978857.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKQGEPDPESN
                     SALKFVLDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADTVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
BASE COUNT        10384 a         6577 c         5081 g        11053 t
ORIGIN      
        1 caattgataa atagcttttg atttatcagg taaacttcct agtaaatttg gatatttagc
       61 taccattttt ttaataaacg tttcatcttg aacaagtttc aagaaatcat cagctttata
      121 aggtgtactt gtagtctttg tatcaatttt attagactca atagctttag caatctgttt
      181 aatagtgtaa ccctcggtaa ttaatatctt acctaaagct ggttttgtcg gttctgcagt
      241 tccgcctttt tccaattcag cagcaatctg atctaaagtc atactttttt taagattgta
      301 gtatccactt tgaaaattac tatagttttt gaacttggaa tagtaattaa agactgtggc
      361 gctcttaatg actcctgctt tttctaaaat ttgaccaatt aatttattcc cagatcctat
      421 cggaatttca acttgtacaa atttatttga attggaatca acaggtttaa tagcactatc
      481 tacaaaacga taacctacaa atgctgtcaa gagcaataca agtatgataa ttgatatcag
      541 aatacgtgca attcgttttg ccacattatc tgctttctga cgtctttcac gcttgttatg
      601 tttgttcttg gctgacaaag ctataccctc ttcttctttt gacaaatcct ctactcggat
      661 aggttccttt gctacgggct ctaactctga tgtctcacca agttttacat tttcaccttc
      721 tgtaggaata tctgttgctc tcacaagaaa gatttcttcc gtttcttgat taggcacagc
      781 agttgcaaca tcagtcttat ctgcttttaa atctatgtca ggtacatttt cagttgtcct
      841 aaattcacta gatttggagc cgctatcaag ctctcttgaa ggttccatcc cttgattgat
      901 aacttgttct tcttgggaag ctaatgcttg ttccgcctga cgtctttctt cttctcgagc
      961 agcttctgcc agaagtttcg cctcaatccg agccttctct tgctcttcta agcgttgctt
     1021 agtctctagc gcctttgctc tagcttcttg ttcgtctttc aaacgttgcg cttcataatc
     1081 tgccatcaat tgtgctgttc tgcgagcagc ttctttggct tcttgctctt tttgatagag
     1141 ttcttcttca cgcaactttc tcaaacggtt agcttcttct aactctgcca gaatttgttc
     1201 tttaaaactt ttctgatcat ggttagaatg ctgatcgtca ttaaattcgg tcaaagagat
     1261 ttcctcctaa taataatcac aggcaattat atcttaaata gtctataaaa tcaacaaaac
     1321 tttgacaatg ttatctactt ttaacaaatt gatcgtgttg gattattcca aatcatatta
     1381 taccaaagcg aaccaccatg ttgggagtca gattctccct catctttaaa tccattcatt
     1441 tcatagaagg aaattaaatc atcatggcaa gttaaactta taccatctcg ttcttgagaa
     1501 acaaccaggt ctttcatcgc tgctaataat gctgtaccaa tcccctgacc tttaaaatca
     1561 ggatgtattg atagactagt aattcctatg aaaccaccta cccttacagg gaattcagaa
     1621 actttatgga ataaatcgtc cgttaagtac cgacctttaa taaccggtcc ttcaatgtaa
     1681 cctgctaaac gaccattaat ctctgcaact aaaaaagtat cagtcattat cgttaaacgt
     1741 tcttgcattg cttttttact cgcagcctct gcttctgaaa aatttatttg ttcaatacta
     1801 atgacttgct gtaaatcctc taggcaacca tttctaataa tcatactcat ctccttaatt
     1861 tctttatgaa attctcttga taaagtcaga tagaggactg agaaacttct ctcagccctt
     1921 accatcaact tattgtgtat ttttagttag gttagctaat aattcttcaa aagagcgctc
     1981 atacaattga atgtctccag cacccataaa gacatagaca gcattatcat gattgagtaa
     2041 aggcgagaca ttttcgactg tcactaaatc tgagtgtttg acaatcttag cagctaaatc
     2101 ttctaccttc acctcaccat tatctacttc tctagcagaa ccatatattt gagcgagata
     2161 aacgctatcc gcttgactca aggcatgggc aaattcgtct aaaagagcta tcgtacgagt
     2221 gaacgtatgc ggttggaaaa tagctacaat ttcttttgac gggtattttt gtcgagcagc
     2281 atctaatgtc gcaataatct cagtaggatg gtgagcaaag tcatcaataa tgacagtatc
     2341 gtcaataatc ttctcagtaa aacgacgctt tacccctgaa aatgtcttca aatgctcagc
     2401 tactaatgcc atatcaattc ccattatgta aaggttagca ataacagcag ttgcatttaa
     2461 gatattatgt ttaccgtatg ctggtacatg aaactgacca atttcttctt ggttatagaa
     2521 aaccttaaag tcagaaccat taacagttcg agtgatgtct tttgctataa aatcatttga
     2581 atcttcaaaa ccataataat atattggtgc ctcagaagtg atttcatgaa gttttggatc
     2641 ttctccataa atgaataaac ctttttgaac ttgcttagca tagtcattaa aggcattgaa
     2701 tacgtcctct aggcctgtaa aataatcagg atggtcaaaa tcaatattgg taataattga
     2761 gtattctgga tggtacggca taaaatgacg ttcgtattca tcagcttcaa acacaaagta
     2821 attagcatta gcagaaccac gtcctgtacc atctccaatt aggaaagaag tgtctgtaat
     2881 attttttaaa acatgagcta ataaacctgt cgttgaggtt tttccatgtg ccccagctac
     2941 acctagacta gtgaactgac gcataaaatc tccgagaaat tcatgatatc gtttaaaatg
     3001 atagcccttt tcaataacat aagccaactc ttcattgtta tctggacgaa aagcatttcc
     3061 tgcaataatc tctaaatcct cactgatatt attcggtgag aaaggtaata tagttacacc
     3121 tgcttgctct aaaccacgtt gggtaaaata atatttgtca acgtcacttc cttggacgtt
     3181 atgtcccatt tgatgaagca tcagtgctag ggcactcatt ccggatcctt taataccaat
     3241 aaaatgataa gtttttgaca tggtctctcc ttaattaatg tctttaaaac gtgtcagatt
     3301 taattcttga gccttggctc tttctttatg aaactgattg tcttgtttat tgtaaacttg
     3361 actgcgtttc aaaaaatcaa aattattttt ctttgttttt gggttcggtg tattatcagg
     3421 ttcttgaaat atgattggaa tttctgctaa tatatagctt tcctgctgta aacgatcaga
     3481 aagatgactt agttcagtca cctttcgctg agtttgtttt ttcggtactg ttgccttcaa
     3541 ttgttgctga aaagcttcct ttgatggcgc cttaaattcc tgtttcgcat agtaagcttg
     3601 tcgtttctcc cgaacagacc tctttgcctc ttgtcgagat ttgctcaact ctgtcgcttt
     3661 ttttacaaaa ctctgcggtg gtacaggatc attttcaatt accttaggat gtgatatttc
     3721 ttcatgttca taattgcttt tgaccatgct ttgatatgtt ttttcttgat aaaagcctcg
     3781 aatgttgtta attaaatctt cattatcata aagattcatc tttacaagcg gagacattac
     3841 tggttcatca tctgctatca acggaaatcg tctagagcct ctattcacat tatcacttcc
     3901 tttctacaac agacctatta taaactaatt tttctgtttt ttcaaagttt tcaactaagt
     3961 aaagcttatc agattggtat ttttcattca tttatataaa aaagaaagcg agagaccact
     4021 ttctttttta tattatagtc acatcatcat atttatttag aaactccaag tatctctcga
     4081 atatcatcta cgctcatgct agcatgggtt tcatttccat ccagaactgt tgtaaccaaa
     4141 tgttttttag tttcttgcat ttctagaatt ttttcttcga ttgtgcctct agtaatcaag
     4201 cgataaacct cgacattctc tttttgcccc aacctatgtg cgcgactaat cgcctgcatt
     4261 tcaacagctg gattccacca caagtcaatc agaacaacgg tatcagctcc tgtaaggttg
     4321 agaccaacgc caccagcttt taaagaaatc aagaaagcat cttttgatcc tgcattaaat
     4381 gctctggtca tttcatgacg ttcatttgct ggtgtagaac cagtaatttt ataagttgta
     4441 agccccatag ctaccatctc acgttctgcg atatctaaca tacccctgaa ctgagaaaaa
     4501 atcagagcac gatgtccatt ttccttaatt tgtgttaaaa gttgacgtag gctttctaac
     4561 ttaccgcttt caccatcata atccataaaa agacgtggag tgtcacaaat ttgacgtaat
     4621 cgtgtgatgc cagataaaat ttcaatttta cgacgattta agtcagcgtc actagaatgt
     4681 tggatacttt cttgaatttg gcgtaattgc gccaaataga taactttttg actgtctgtc
     4741 atttcatttg gatagttcat ttcaatcaaa tccggtaatt ctgggagaac ttcttctttt
     4801 cgacgtctca tcacaaatgg tttgatataa cgagcaactt gtttagggtt caacttcaaa
     4861 aactctttct taccaggtaa taaaccaggt aaaatgatct ggaaaatgga ccaaatttct
     4921 aacagtttat tctcaatagg tgttcccgat aatgcaaaac agtttttgac ctcaaaagat
     4981 ctcaagctat gggctatttt agtttgagca tttttcataa cctgtgcttc atccaaaatt
     5041 aaataatcat aatttccttc agaataagtc tcaaaatcct gcctaaatga tgaataactt
     5101 gtaatgatta cttgatgacg ttcagcaata atttcatctc tacgaatctt agaaccatag
     5161 gcaactgcta catctacgtc tggcgcaaat ttgtggaatt catcttgcca attataaatt
     5221 aaacttgatg gtgataaaat aagcacacga gaatctctag ttagttttgt acttaaaaat
     5281 gaaatcgttt gaagtgtttt tccaagcccc atatcatcag ctaaaatccc tgcaaagcca
     5341 taatgatcta acatacttaa ccaacggaca cctgtaagct gataatcgcg caaggaagct
     5401 gaaacagata gaccaggtat tttaaacttt tctgggtgtc tcaaatcttc aatcaattgg
     5461 taaaaatgtt gtgaaaagct aacattatct tgatttgcaa acagttctga taattgaaat
     5521 gctgcaatat tatctaattc aatatgccca ttctttgctc gtctagctct caacccttgc
     5581 aaagttgcac ttactttctt agtttcttca tcaaaaataa ctaattgccc tgatttattc
     5641 acaaaataag gattattgtc aaacaaagct accagagctt gatcaacatc gttttccagt
     5701 aaatcagaga aatcaaaaga aatatctagc aggcttccct tagcttcaat atcaatttgc
     5761 ggtcgttcta tgagacgata actttccaat tttgcggata agctcactgt tcccattttt
     5821 ttaaattgtg gaagcatata ggtataaaaa tcgtataatt cttgtgcact gtatgctgga
     5881 tggctggttg aaaagtgggg tgcaaagcca tatttttcta agagtttaaa tactttttct
     5941 tcatgtttga agtgactagc aaatgttaac tgttctagtt cttgacgatt atgaacagtt
     6001 aaatcattac cataatcaaa taccatttgc aataaaattt cattttgact attaatatcg
     6061 aactcaaaat ttaccttaaa atcatgaata gagaagctcc gtggagcatc cactagtcca
     6121 atctccttaa aatccagtaa atgagctgct aacttatctt gatcatctaa atcaaaatgg
     6181 atatgttttg ctaaatttcc ctcaatggga agactacgaa tagccgttac catcttttct
     6241 tgcttcaaat taagatggta gaaagtatcg cgataaaaca aataagaatt cgcaaatagt
     6301 ggttttaagt ctttctcttt gatttctaat tcaattgatt gcctatggac aataacctta
     6361 aattgataaa gatgatcctc accttctaat tccttaaagt agaggtgatt aaattgatga
     6421 aaatcacttt caaatgaaaa attttctaaa gcattcatca aagtaacacc ctcttcaaaa
     6481 aaaccacgag tgagactaag gtgacgacct tgatttggta aaataaactc tagatcaatt
     6541 ttgcttgaat gacttggaat gagcctccat aaaaattcta tcaattcttg actggtctca
     6601 tcaaactgta tcaaagacaa agtttcaaag tattgttttc caatctgata ataagcttct
     6661 tttcgaacag tatttaaaaa ggctttgatg tcacgaataa cataacttct atcatcaggc
     6721 agtcgtctaa tttttaaagt ccaccagata tcattagcat aaggattttg ttctccttga
     6781 gccgataatt gatattttat agtatcatct tcattaataa ttagactatc taaaaaaaca
     6841 ctaccaaaag aggttttctt cttagtttct tgttgacttt cttgttgaac ttgaacttta
     6901 gataagattg ctttaccttc tggatcgttt ttcaagaaat gctccagagc agctaaatgt
     6961 tgacaatagc cttttcttgc aaaaaaatca caagaacatt ttgtttcttc tgttactaat
     7021 gaatattcaa tctgacaatc cccaacttta gcttttaata gattaccttc ttgagaaatc
     7081 aaggacacca aaccttgttc gtagagttct ataccttgat ttcgaatacg tcctggaatc
     7141 attctactca ttatgctacc accttcatct tatcccttaa ttataacaaa attcaagtcc
     7201 aatttcttgt aaaaatacta aattgattgt tttttgatac agtaacaaat aaaaaaggct
     7261 aaaacaactg tcctaacctt ctatttcaat ttcaacaata agatgttatt tacgtttacg
     7321 tgctattaga ttaattggtg ttccttcaaa aacaaatgct tcacggattt gattttctaa
     7381 gaaacgaaga tatgaaaagt gcatcaattc ttcttcgtta acaaaaacaa cgaaagttgg
     7441 tggtttaaca gcaacttgag ttgcgtagaa aatcttaagt cgttttcctt tatcagttgg
     7501 tgttggatta atagcaatag catccataat cacatcattt aatacagcag acggaatacg
     7561 tttgttttga ctttcgctaa tacgtttaat catatctggt aacttgtgca aacgttgctt
     7621 tgtctctgca gaaacaaaga taattggcgc ataagacaag aattggaagt tatcacgtat
     7681 atcagcttcc cactgactga cagtatgatt gtctttttcg atagtatccc atttattaac
     7741 tacaataata atacctttac cagtttcatg tgcaaaccct gcaatacgct tgtcatactc
     7801 acgaatacct tcctctgcat taatcaccat aagaacaaca tctgagcggt cgatggcgcg
     7861 catagaacgc ataacagaat atttctctgt gttttcatat accttaccag atttacgcat
     7921 accagcagta tcaatcatag tatactcttg cccctggcta tcaacgaaat tagtatcaat
     7981 agcatcgcgc gtagtaccag caactgggct agcaataaca cggtcttccc caaggatggc
     8041 attaattaaa ctagatttcc caacatttgg acgtccaatc aaactaaaac gaataatatc
     8101 tggattttca ttttcctctt ctaccggaag attttccaca atagcatcca atatatcacc
     8161 agtaccaata ccatgaactg atgacaaggg ataaggatcc cctaaaccaa gagagtagaa
     8221 atcgtaaata tcattgcgca tctcaggatt atctacctta ttaacagcta aaataaccgg
     8281 tttattggtc ttataaagaa tacgtgagac atactcatct gcatcagtca caccttcttt
     8341 acccgacaca acaaacacaa taacatcagc ctctgtcata gcaatgtctg cttggtgctt
     8401 aatttgctcc ataaacggtg catcaacatc atcaatccca ccagtatcaa ttaatgaaaa
     8461 cttacgattt agccactcac ccgttgtata gatacgatca cgagtgactc cttcaacgtc
     8521 ctcaacaatg gaaatacgtt ctcctgcaat tctgttaaat aatgttgatt ttccaacatt
     8581 cggacgacca acaatagcaa ctgtaggtaa taccataatt ctcctctcac tattgggaat
     8641 actcccaatt ccattctatt ttattaacgg cgattcggtc cttctaagtg gaattctatc
     8701 gctaaatacc gtacgcgctc cataactcgt tttgcttgcc aagtttcgtc gcttcctttt
     8761 atattagccc attttctttc caaatcgtta aaactataat ttgacgtaaa gaaagtgggc
     8821 aactcttcta gcatacgatg ttgtaatata acttgtaaaa tctcgtcacg aacccaggac
     8881 gttgcctgtt cagccccaat atcatctaaa atcaaaatcg gaacactttt cacagcatca
     8941 atttcatctt ttacagttcc gcttgaaata gcatttttga catcaatagc aaaactaggg
     9001 aaatgaagaa gagtcgttga cacacccttt ctctctgata attccctcgc cattgctgcc
     9061 attaaatagg atttcccaac ccccatatct ccatataaat aaagcccctt gtgattatat
     9121 gaaggataag tctctacaaa atcaagcagt tgtgacatag ctttcattcg actctcgtta
     9181 ttaatatcaa agtcagtcat tctgatattc cgataggact tgggtagatt aactaaatta
     9241 atacgatcac tgatagcttg ttttttttgt gcttcaatta actctcgtgt ttctaaataa
     9301 gaaacatctg cataaccttc attcataacc aaaataggct catagccttt agcaatatat
     9361 tgactatcct tatttttaaa tttctgacgc tcaatcaaaa attgattaaa tttagacatg
     9421 ctgatattta tttcacgctg agaaagttgg tgggttttaa tgaactccgc tacctgagca
     9481 tctgctaaaa tcatttgaat aagctcatca tttgtattac gaggcactct cccttgattt
     9541 tctaatgctt gtcccactga tttcatgatt caccatcctt tcctaatcgt tctaaacgct
     9601 tgagggcatc tgttttaaac tgttctaatt caatttcttt ttctgggcta acctcatctt
     9661 tataatcagg attactccat tcaggaacat tcgattgttt cttcttcgtt tctgttttag
     9721 tccttacttt ttgatctgaa aaatcacgaa ttttaagcac agcatcttcg gccgttataa
     9781 cattctgaaa ggcaaaatca ttagcaactt tcatgatata ggccttattt aagttagcag
     9841 attttgtctt attcaaggta tataaaacca taacattaat gacttcgtct aggaaattca
     9901 tttttgccaa atcctcaagt aaagtcttct caccacttgt tgtcactgcc ttacggcttc
     9961 tcttgatttt ttcaaggaac actagagcac tatcattttt agattccctt agtatgacct
    10021 tttcattatt tgtaaaactc tgattatctt taatatgctg cccctcattt tgttgaagtt
    10081 tcattcgctg aggcgcaatc attccattga tagctgtttg ttttgctaat tgataggtat
    10141 cataccaatt taaatgataa agttctgata cactataaat acctaagaca tcatcttttt
    10201 cattgttaaa acgtaagcca tctcgcatca tcaaacgttt gaaactttcc aagtcaaaaa
    10261 catttttact ccggtttacc tcttgttttg gttgtcctaa atccgagaaa acatctgtaa
    10321 attttttaga gatatctcta gcatttttag gtattttcat atctaattct gccactgcta
    10381 cctctcctat acgtttttct aacaaacgac gatagatggg attagataaa aataactcat
    10441 ttgacatagc ttgattcaac ttaacaaggt aagtacctgt ggcttggtaa acagtcaaca
    10501 aatccaaagc tgttaacatt accaatgcat cttgaaatct tggcatcccg tattgcagat
    10561 gattgagaac ctctgaaaat ttatgacgtc gtattccgtc atcaaaaaaa tgaacaaaat
    10621 aatcatagag tgccatggca tcacttccca taattgggaa atacaattgt attaaactat
    10681 tactatcata aacaatctta ttttgcttaa tatatgtaaa ctcatctatt ggactcatca
    10741 cacgctacca ctctttttac tccgtacgcg ctttgtaatt tgttgaagta gttcttcaat
    10801 ttcatctaca tctttaaaag atttataaac actagcaaag cgcacataag taatctcatc
    10861 taattcggcg agttcatcca tcactaaatt tccgatagtg atactagaaa cctcatcttc
    10921 atactcactc ctaattttac gttcgatacg taagatacaa ttttcaatat cttcgctcga
    10981 aacagggcgt ttttgagcac tctgaataat accattcaaa attttatctc gagaaaattg
    11041 ctcgcgagta ccatctttct ttatgactaa aagaggcaat tcttccaaac gttcaaatgt
    11101 tgtaaagcgg ttgccacatt tttcgcactc tctccggcgt ctgatagtcg taccttcttc
    11161 agcttgtcgg ctatcaacca cactagattt attatagcca cattttggac aacgcatgtc
    11221 aacaccccta ttctatcata tcatttaatc atcttatttt aacacaaatt cagtaataac
    11281 aaaagaagcg cctctcttat cacctcatca tatcaaaaca caaaataatc cttatgcctt
    11341 ttatcattaa gcataaggat ttttcttata tttctttagt ttcttcaaat ttttttatag
    11401 gaatgcgaac aatgaattca gtcccttgat ttagttcact tttaatatcc acctttaaat
    11461 ggtaagcatc cataatttgt ttaaacactg aaagcccgat acctaaacca gcttgtgtac
    11521 tttctctgtt acgtgattta tcagttcgat aaaaacgatc aaagataaac tcaatatctt
    11581 catctgagat accttctcct ttatctttta caactactgt cgcaaaatca ttgtctactg
    11641 ataaaacaac actgacttct ttataactag gagagtactt gatagcgtta tcaattaaga
    11701 tcatgagagc ttgctcaaaa tgtatcttat atatctttcc ccaaacgata tctgaaatat
    11761 tattttcaaa aataaattga aaatcttctc tcaaaatacg gaaattttca ataacaactg
    11821 atatagagct tgataaatct gtcacttcat cttgatgaag ttccaagctt ccttggacgc
    11881 gaatcatatt taacatgtca ttaatcatta aggacatacg atctgcctca tggtatgccg
    11941 ctgctaaact ttcttcaaga atttctggat catctttccc ccaacgttgt aataaaccaa
    12001 tatgaccctt tacaacagcg acaggcgttc ttaattcatg actaacatca ctaatgaatt
    12061 gtgattgtcg acgagtatag tcttccaact gatccaacat cccatcaaaa atcctagaca
    12121 attctccaat ttcgtcttct gaacggacct tacttctaat ttctaaatta ctaggatttt
    12181 cagatatctg atgcattaag tcgtgtaaat ttttaaccgg acgaacaata cgcttagtag
    12241 cactattaat aacgaccaag gctaaaacag tccctaaaac ttccatcata atcaagaaaa
    12301 caatgatgtt ttgcctcata ctataataac gacctaaatt atagaaaatt tgaacgtatc
    12361 caagcaactg tcctgttttt tgagagtaga ttgcttttgt accaacaaaa ccttccatat
    12421 ctgcaacttt agagatgata acttttccca tggatttacc aatagttggc atacccgttc
    12481 tcagtgtaga aaaaactggt ttcctgttga catcgtatag ggtcatttct tgattaacat
    12541 aaagaatact atcgattgac tcgcccgttc taacaattcc attttgacgc ttatgaggat
    12601 agatgtcatc cgcaaaaacc tggttgtaca ggatctctaa aatatcatcg gatgtcaaac
    12661 tagagttcgc ttgcgacaaa cgaactctag ttttttctaa tgacctaccg acattaattt
    12721 tttccctgtg aaggacgtat cttgttgccg aagtataaac taaaacagaa aatactgtaa
    12781 ataggctaaa aaataagata aaaactaatt gcgataattt ttttgacaga ggttgtttta
    12841 cccctataaa ctgatctttt ttatttttca cgaatcacat agcccattcc gcgaacagtt
    12901 tgaatatatg attcacggcc tgggatatca attttacctc ttaggtaacg gatataaaca
    12961 tcaacaacgt ttgtctctgc tgccacatcg tatttccaaa catgttctag caactcttct
    13021 cgtgtcataa cacgattcat atttgtcatc aagacattca acaaatcaaa ttcacgtttt
    13081 gttagtgaaa tttcctcatc accacgcatc gctgaacgat tatgagtatt taatgacaaa
    13141 tcacggaaac taccactatc acctttttct tttgtcttcg tttcaatttc ttggcgtcgg
    13201 aaaatcgctc taacacgtgc taataattct tcgattgcaa acggcttaac aatataatca
    13261 tctgctccac gatcaagacc agctacaata tccataacag aatcacgtgc tgtcatcatc
    13321 atgatatagg ttgttttttc agcctgcagg cgacgtgtga tttcgaaacc atccatctct
    13381 ggaagcatta aatccagtag aatcaaatca aaatcttttt ctaatgctgt gtccaatcct
    13441 tcacgaccgt ttgtttcaac aacaacatca tatccttcat gtagtagttc taacgagacg
    13501 aagcgagcta aatttttctc atcttcgatt attaagatct ttttacccat ctacaccaac
    13561 cttcttattc tttacttaac actatcaatt attttttaga atatctagat aaaaaacaag
    13621 atagcccaca ttttactaac gtttctttaa ataaataaat cgccatccca aacaactgtt
    13681 cgatagcgag acttaatttt tattattgag tagtgtcctc tgtcactttt caattctact
    13741 aaaaaaaaat taaaatatca actgatgtta ttaataattt ttatctaatc actatcaaac
    13801 ataccttcta aggctgcgaa aggattagat ttttcttttt ctttagcttg tttttcttca
    13861 tactgctttt cagtcattaa cgaccaattt ttacctgata aatccgcttc taccccaacc
    13921 tcatcagctg ccagaacacg aagaggtata tttaacaaga tattatcgat aacgctttct
    13981 tctaaattaa tatcatcttc ctctaatatt aagaccaact cctgatcaac gagctctttt
    14041 ttagtgctaa cattttcagc ttcaataaaa acttcattaa tagtttgctt ttcagataaa
    14101 acaactggtt tcatagaacg actagacggt aatgtaatat cgtaagccat attatagtca
    14161 agtagataga gaccatcttc atacaccaca tgacctgaaa cttgaatatc agaaatatcc
    14221 attatctctg aatgacgctc cataagcgac tctttgatat ctattttttt atcaaaataa
    14281 agcccctctg gtgatttttt gatttctgta agtaacacgt gccatctccc tttatagtat
    14341 atgtcatatt atatcaagtt ttacaggctt tgtcttaaaa aaacaaaacg caaggcatag
    14401 ctaccctacg ttttgttaac gactacatat gacgcaaacg ctcaatacga tctgcaattg
    14461 gtggatgcgt ataaaataaa gactttaacc cttctttttt cgttgggtca ttgatataga
    14521 gagccgcact agcatcatct actggatgcc ccataggttc agaacgatca agtttttcta
    14581 aagcagagat cattccttgt ggatttcttg ttaactcaac agaactagcg tctgctaagt
    14641 attcgcgctg tcttgaaata gctaattgaa ctaaactagc ggctaatggt gcaaggatta
    14701 gtgataaaat agagaaaatc aaaaccaaga tgtttccacc atcttctctg tcatcatcac
    14761 gcctacgacc tccgccgtaa aataacatac ggctaccaat gcttgatatt agagttacag
    14821 cactcgctaa agctactgca atggttgaaa tacggatatc ataattacga atatgactaa
    14881 cctcatgtcc aataacaccc tctagctctt cgcggttcat aacagctaat aggcctgtcg
    14941 ttgctgcaac agcagcattt tctggcttag agcctgtggc aaaggcattt agggagtcat
    15001 cttcaacaat gaaaacgcgt ggcataggta tctgagctat cattgccata tcttcaacaa
    15061 tatgaaaata atttggcgcc tcatcttctg taacttcacg cgcattgttc attgacataa
    15121 caacattagt tgattgaaaa atcatactta ctgcatagat aaccccaata attagagcta
    15181 gaacaagccc aaattggtag ctgcctaata ccaaataacc tacagctgct ccaatagctg
    15241 ctaaaagaca gaagaagaca attaacaaga caactgtttt tctcttatta cttgcaattt
    15301 gttgatacag caaaagcctc tctcccttct caattaaaag tcaaaggaaa cttttggcac
    15361 ttctttttct tcttcaggag tttctaagaa ttggcttggt ttgaacccaa atagttttcc
    15421 aacaatatta cttgggaaag tttctaattt cacattataa ttggatgttg ttgtgttata
    15481 taactgacgt gaataagaaa ttttattttc cgtattagtt agttcttctt gaagtttaac
    15541 aaagctattg ttggctttta aatctgggta attttcagca accgctaaaa tacttgaaag
    15601 ctgtcttgtt agctcattac ttgctgtcat tgcttcagca ggtgtgttag ctttagcaac
    15661 ttgagcacgc agttctgcaa tcttctcaag tgttttccct tcgtacgcag catagccttt
    15721 aacagtttcg attaggtttg gaattaaatc gttacgacgt ttcaattgaa cgtctatttg
    15781 actccatgat tctttagtat gcatgcgact tctaactaaa ctgttataag caacaatcaa
    15841 ccaaataaca aacaaagcaa taattgctat taaaatcata gttcccatag tagtagctcc
    15901 tttacatttt ttccattata gcaattttct ttagattagg aaagtcctgt tattcccatt
    15961 attagtattt gtgataaaat agagtcatta tacgatatag aaattgaggt ttactatgac
    16021 accccaagca ttttatcaag ttctgataga gcatggtatt acacttactg acaaacagaa
    16081 aaaacaattt gaaacttatt ttagacttct tgttgaatgg aatgagaaaa ttaacctaac
    16141 agctattaca gataaggaag aggtttactt aaagcacttc tatgattcta ttgcgccgat
    16201 tttacaaggt tatattgaca atagtcctct tagcatttta gatattggtg ctggtgcagg
    16261 ttttcctagt attcctatga aaatccttta tcctgaaatt gatattacta ttattgattc
    16321 actgaataaa cgcattaatt ttctaaacat attagctaat gaacttgaac tttcaggagt
    16381 tcacttcttc catgggcgcg ctgaggactt tggtcaagat aaagtatttc gagctaaatt
    16441 tgatatcgta acagctcgtg cagtagctag aatgcaggtt ctagcagaat tgacaatacc
    16501 ttttcttaaa gttaatggtc gcttgattgc actgaaagct gcagctgctg aagaggaatt
    16561 aatttctgca gaaaaagctt tgaaaacact attttcacaa gtaactgtaa ataaaaatta
    16621 taagttacct aatggtgatg accgaaatat cacaatcgtt agcaagaaaa aagaaacgcc
    16681 taataaatac cctcgtaaag ctggtactcc aaacaagaag cctttgtaaa gttgggagct
    16741 agtatgaaac atttctttga ttacaagaca atgtctgtcg cacgtaaact cagcattagt
    16801 tttatcgctg ttatcctatt gggaagtatt ttactttcac ttccaatttt tcaatatgct
    16861 aacgccccta aaacacatta cattgatcac cttttcacga ctgtttcaat ggtatgtgta
    16921 actggtttat ctgtttttcc tatcagtaag gtttataacg gttgggggca gattgtagct
    16981 attttactca tgcaaacagg gggacttggt ttagtaacac taatgtcgtt gtcttactat
    17041 accctaagac gtaaaatgtc gttaaatgat cagacccttt tgcagtcagc gataacttat
    17101 aacagttcga cagatttaaa aaaatatctc tatatgattt ttaaagtaac tttaacttta
    17161 gaagttttag cagccagcat tttagccata gattttattc ctcgatttgg tttgggtcat
    17221 ggtatcttta atagtatatt cttagctgtc tcagcctttt gtaatgccgg ttttgataat
    17281 ctaggggcaa cgagcttagc tcaatttaaa ctaaatcctt tggttaacat tatcgtttgt
    17341 ttcttgatta tttcaggagg acttggattt gccgtatgga aagatttaat tgaagctact
    17401 atacaaacat ctcacaagga accaaaatta atcaagacat ttccaaaacg cctcagcaac
    17461 cattctaaac tggttttaaa aacaacaacc attattttac taacaggaac tttgctttct
    17521 tggttgttag agtttggaaa ttttagaaca attgctaatt tatctctacc taaacaacta
    17581 atggtcagct ttttccaaac agttacgatg cgtaccgctg gattttcaac tattgattat
    17641 acccaaactg atttcgcaac caaccttgtt tatattatcc aaatgttaat tggaggagct
    17701 cctggtggaa cggcaggagg atttaaagtc actgttatcg ccattttact cttactattt
    17761 aaagccgaac tgagtggcca aagccaagtg acttttcatt atcgaacaat tccttccagt
    17821 attattaaac aaacactatc gattttaact ttctttttta tcatacttat tagcggctat
    17881 ctccttcttc tggaactcaa tccccatata gatccttttt ctcttttctt tgaggcatct
    17941 agtgccttag caaccgtggg tgtaacaatg aacactacca atcaattaac tctgggcggt
    18001 cgaattgtaa ttatgttcct catgtttatt ggtcgagtcg gtccaattac cgtactgcta
    18061 tcaattcttc agaaaaaaga gaaggaaatt cactatgctg aaacagaaat tattctaggt
    18121 tagaaaaggt cttttatgaa aacaaaaatt attggtgtac ttggtctagg aatatttgga
    18181 caaacactcg cacaagaact aagtaacttt gaacaagatg ttattgctat tgacagcaat
    18241 cctgaaaatg tacaagctgt cgccgaagtt gttacaaaag cagctatcgg agacattact
    18301 gatttagctt tcctaaaaca catcgggatc agtgactgtg atactgttat tattgctaca
    18361 ggaaacagtt tagagagctc agtattggcc gtaatgcact gtaaaaagtt aggcgtccca
    18421 caagttattg ctaaagctcg aaaccttgta tacgaagaag tactttatga aattggtgct
    18481 gatttggtta tctctccgga gcgagaatct gggcaaaatg ttgctgcaaa cctcatgaga
    18541 aataaaatta cagatgtctt ccagattgaa tctgatattt ctgtcattga atttaaaatt
    18601 cccaaatcat gggtagggaa aactgtcgaa caattaaata tccgtcacaa gtttgatctt
    18661 aacctcatag gtatcagaaa agcaaaaaac aaacctgtcg ataccgaagt tccaatcaat
    18721 actccacttg aagaaggtat tattctagtg gctatcgcta actcagatgc cttccaacgc
    18781 tatgactatt taggatactt taactaaaga aaataaaaaa ctgactatta aagtcagttt
    18841 tttattgcca tgggttatag aaacgtccat gattaaggtg aattaaataa aggcttacaa
    18901 acagactagc tagagcaagt atgattaaga caatgtcact tttttccaaa gattgcttag
    18961 agtaccaagt acgtctttta tttttaccaa atcgtcgaag ttccatagca gttgctactg
    19021 tatcaatacg ttctagagag ctaaaaatta atggtgtgac aatctgcaaa ttccccttaa
    19081 tgcgtgctac taagttcgat ttttttgata attctattcc tcttgcctct tgtgctcgac
    19141 gtattgtata aaattcttct tgaacatcag gtatatatct aagggtcaaa ctaacagcat
    19201 aagctatttt atatgataaa cctaattgat ttaaacttga tgcgaactga ctaggattag
    19261 ttgtcatcaa aaacaaaaga gctaaaggca ctgtacaaaa gtattttaaa atcagattaa
    19321 agaggtaaaa caactcttgg ctagtcacat catataggcc aattcctttt aacaataaag
    19381 aagaactacc ataaatctta tcaccataat ggggagcaaa aagatagacc attataatat
    19441 ttaaaacagc gaatacaaca actaaagtca taacaaagga cacctgttgc catttgattt
    19501 ttgctaatct aaataagact agcgatccca atgaaacaac aattaaaaat cttgtatcat
    19561 aagtggtcat gcaagcaaca gaaactaaaa taaaaaatag taacttactg gcacctgata
    19621 gcctgtatag aaagccataa ccctcttgat aaccaattaa ttgtttagcc attcttacct
    19681 ccttgattat caatgtacca ttgtgttagt ttctgtggag atatccctaa tctgtctgct
    19741 aaagcaaata aactcgtttt ctttaaatga gtctttttca aaagctctgt atttgaaagg
    19801 atgttaatag gatgatcatc tgcaacaatc agaccattgt cgattacaat tgcacgatct
    19861 gtatactcta acataagctg catatcatgt gtaatcataa caatggtatg tccatcacaa
    19921 cttagtttat caaggaaaga catcatttca gtataatgct tcatatcttg accagctgtt
    19981 ggttcatcca aaataataac ttctggattt aaaatcagta tcgaagcaat cgtaacacgt
    20041 tttttctgac cgaatgatag agccgatata ggccaattcc taaattggta gagcccacaa
    20101 acctttaata tatcgtagac acgactttct atatcattat cactaaaacc tcttaaacgg
    20161 agacccagag caacttcatc aaaaaccatc gcttgactta tcatctgatt gggattttgc
    20221 aaaacatagc caattcgttc tgctctttct tttatagagt cttgactgac atcttcatta
    20281 ttataaatta gagtagcatt attagggata aattgacata acgctttagc taaagtagat
    20341 ttacctgcac catttttccc aacgatagct aaccgctgac ctttatataa atcaaacgaa
    20401 acatcatcta aagtattatt ctctaaatca tatgacaccg acaaatgctg tactgataat
    20461 aatttatctg tatgtttatc taaatctaat gctcgcattt taggtatcaa tagttcactg
    20521 aaatcaaagt catcaataga cgtcattgta ttttgttttt caaagtcata tcctaaattt
    20581 ttgagagcag aaatatacag tggttctcga ataccatatt cagataaaaa attagttttt
    20641 aaaagttgat ccggctcccc attataaata atttgtccat cactaaaaag aactacacga
    20701 tctactcgct cataaagaac atcttccaat cgatgttcaa taataatagt ggtcgcatct
    20761 tgctcttgat gaatacggtc aaccaatgcc atgatatctt gtcccgattg tggatctaga
    20821 ttagccaaag gctcatcaaa taatagaatt gggctatcgt ctactaaaac tccagctaaa
    20881 ctaacccgtt gtttttgtcc tcccgacaag tcttgtggac gtttagacaa aagaggagct
    20941 atttctaaac gttttgccca catttcaacg atagaggcca tttcttcctg tgctacaaca
    21001 tcattttcta atgcaaaagc aatatcttct gctactgtca atcctataaa ttggccatct
    21061 ggatcttgta aaaccgtaga aactaaatga gacttgtcat atattgacaa gtcaaaagca
    21121 tttttatggt taatcgtaaa aataccacta tattggcctt tatgagtatt tgggataata
    21181 ccatttaaac aatgaccaag cgttgacttc cccgaccctg aaggtcctaa tatcaagacc
    21241 ttttcacctt taggaatgct taagttaatc ccttttaaag ttggttcaga ttgaacgtca
    21301 tattgaaagg taaaatcctt ccattcaata aaatctttca tagattactc tcttatccta
    21361 atctttggat aaactacctt tttgtggtcg tgatttagcg tatgctagca acaaaagcgt
    21421 accacctact ccaatagtaa tggaattcac aaggcttgat aagaaacctt gtgcaaaaac
    21481 tttgcttgca ggttcgctat aaaagaaaat atcaccatac ggtgcaacga cagaccaacc
    21541 tataaggtta gcaataactt gaactacatt aaatagaagc aaatccttct taatcatgcc
    21601 agataattga atagctaatt ttttagcaaa aaagcctatc atcaaaccta aaagaccact
    21661 aactaaaacc caagtccacc acggagaacc atattgaatc gaatctttca atgcgtgtcc
    21721 gataaaacca gtaaaaaaac caactcctgg tccataaata actgcaaata atgctaaaac
    21781 tgcatactgc aattgaatat tagtatttgg aattggtgtt ggtatattaa ctagcatacc
    21841 tataatgata aaaagtgcag ctccaattcc agtcgctaca acctttttta ttgtgtttgt
    21901 attcataaaa acctctctga aaaacaagct taaacagtca cttacaatct gttttatttt
    21961 atcatatttc aagctgattt gaaaccttat tgacaagaag aactgaaaaa cgattgaaaa
    22021 taatattaaa taccaaaaaa gatctcagat agaataccta agaccttcat tagtttattt
    22081 ttttccaaaa actgattttt cagctacagc cgtgaacaga acgtctgtcg atgaatttaa
    22141 agctgtctca cacgaatcct gtatcacccc tacaatgaaa ccaactccta cgacttgcat
    22201 tgcaacgtca tttgaaatac caaataagct acatgccacc ggaatcaata aaagtgaacc
    22261 accagttaca ccagaagctc cacatgctga tacagcagca accacactta ataagaatgc
    22321 tgttggaaaa tcaacagtaa ttcctaaagt atttactgct gctaaagtca ggatattaat
    22381 tgtaatagct gcaccagcca tattgatagc tgcaccaagc gggatagata ctgagtaagt
    22441 gtctttatct aatcctaaat cctcacatag tctcatatta acaggaatat tcgccgcaga
    22501 actacgtgta aagaaagctg taatacctga atcctttaag catcgtaata ccaaagggta
    22561 aggattttta cgcatcataa caaaagcaat gattgggtta acgacaagag ctacaaataa
    22621 catcgtaccc accaaaacta atattaaaag accataatca cctagtatac caataccgtt
    22681 ttctgaaatg ctagtgaaaa ccaaccccat aataccaatt ggggccaaat tgataatcca
    22741 tttgacaact tgtgaagtag catcagcaaa agtttgcatt aaatcttttg tacgctgatt
    22801 actgcttcgc atcgccaaac caaaaataac cgcccaaaat agaatactca tataattggc
    22861 ctcggtaatg gcatgaatgg gattatcaac aattttcaac aaaagagatt gaaagacttc
    22921 tgaaatcccc ttgggaggta ataagtctgt ctttgctggc gttttcaata tcagtgtcaa
    22981 agggaaaata tagttcacaa caacagcgat tagggccgct gcaaaagttg cagtgatata
    23041 caaaccaatt atcgttgaca tattggtctt accgccctca cggtgttgcg acaaggcact
    23101 aataactaaa gtgaaaacta aaagaggggc aatagctttc aaccctccta caaaaagttg
    23161 accaagtaaa ccaatcgcag aggctttagg gactaaaata cccaagaata agccaataac
    23221 gactccaatt gttatttttt ttatcaagct tgtttttctc caagctttta aaaatcgttt
    23281 catactaaac tcctaaaaaa ttcttaaagt ctatgatatg ataaaaaaaa agattcgtca
    23341 aatacctttt ttatttttta gaggttgacg aattctaaat ttttctgata taattacttt
    23401 ttgaaatatt caatcaattt cgaaaataaa atattttttt gtacagaaag gtacattgat
    23461 gaaactaaaa aacacttata tcattatcgg cttgatgctt tttgcgctgt tctttggagc
    23521 cggtaatctt atttatccag cctacttagg tatctactct ggttctaatt gggcgtgggc
    23581 tatcattggg ttctgcttaa caggcgtaac tttaccccta cttggtgttg cagctgtgac
    23641 gtactcagga gcaaccgatg ttgaaagctt caccaaacct gtctctaaaa catatgctat
    23701 tttctttgca gtagcactat acctatctat tggacctttc tttgccattc ctcgaacagg
    23761 ggcaacctca ttctcagttg gtattcaacc tatcttaggc gacagctcct tagtcaaaat
    23821 tatctatggt ttaattttct ttggtatttc ttatttctta gctattagac ctagtcagat
    23881 tgtagatcgt attggtaaat atctaactcc ctttttacta actgttatta gtattttaat
    23941 tattgcctca ctcatccatc cagctgctat cgttggaaca ccacataacg cttcaacgaa
    24001 tgtcagcgat gctttcaagg atttaccatt cctagcagga ctgattcagg ggtatggtac
    24061 aatggatgcc cttgcatcaa ttgcttttgc tattatcgtt attcaagcaa gtaaacagta
    24121 cggagcaatt accaaaaaag aaattacttc tatgacactt aaatctggtg ctatcgctac
    24181 atttctttta gcttttattt atatttttgt aggacgtatt ggtgcaactt cacaaagctt
    24241 gtttaaattt gcaaatggtt ccttcttact tcacaatact ccaatagacg gtgggcatgt
    24301 tcttagtcaa tcagctaact tctatttagg aattgtggga caagctattt taggtacggc
    24361 tatttttctt gcttgcttaa caacagctac tggattaatc actgcttgtg cggaatactt
    24421 ccacaaatta cttccaaaga ttagtcacat tacttgggct acaatcttta ctttaattgc
    24481 tattacgttc tattttggtg gtttatcaga gattatccgt tggtcactac cagttctcta
    24541 ccttctctat ccattaacta tagtcttaat tttcttagtc ttttttgatc aaaaatttga
    24601 aagtagccgc attgtctacc aaacttctat tgccgccact gctgtcgctg ctttatacga
    24661 tgccttatca aaactcggcg aaatgactgg tttatttacc attccatccg ctttgaccac
    24721 attctttact aaagttgttc ctttaggtga atattctatg ggatggatta gttttgcaat
    24781 ttgtggtgtc ttagttggtc ttattctaaa aaaagtaaaa gcataataat ttaaagcagt
    24841 acgaaaatta ctgcttttat tttaaaataa ttattgtaac cgctttcttt tggtggtata
    24901 atagccttga aaataaataa tttttggagg aactctaata tgagagctgc aacttatctt
    24961 tcagctaatg aattatcact tactgataag gctaaacctc aagtaataaa acccacagat
    25021 gcggttgtaa gacttgtcaa aacaaccatt tgtggtactg atctacatat ccttggtggc
    25081 gatgttcctg cttgtaaacc aggaacaact ttaggtcatg aaggcattgg tatcgtcgaa
    25141 gaagttggtt ctgctgtcaa tcattttaaa gtcggagata aagtaattat ttcttgtgtc
    25201 actgcctgta atacttgcta ttactgtaag caccagttgc cttcacattg tgaagatggc
    25261 gggtggattt taggacatct tattgaaggt actcaagcag aatatgttca tattccacat
    25321 gcagatggta gcctttatca tgcacctgaa ggtgtttgtg acgatgcatt ggttatgtta
    25381 tctgatattt taccaacttc atacgaaatc ggcgtcttac cttctcacat taaaccaggt
    25441 gatactgttt gtattgtagg cgctggacca attggactat ctgccttact aactgcacaa
    25501 ttctattctc ctgccaaaat tattatggtt gatttatctc aaaaacgact tgaagcctct
    25561 aaaaaatttg gtgcaacaca caccattcta tcaacgtcta ctcaagaagt taaagaagaa
    25621 attgataaaa ttaccaaagg ccgtggcgtt gatgtcgtat tagaatgtgt tggttatcct
    25681 gcaacatttg atatttgtca aaatgtagtt tctattggag gacatatcgc aaatgttggt
    25741 gttcatggta aacctgttga atttaacctc caagatttat ggataaaaaa tataaccctt
    25801 aatactggat tagttaatgc taatacaact gaaatgctcc ttgaagttct tgagacaggc
    25861 aaaatcgatg caacacaact agtaacacat cacttcaagc tatctgaaat cgaagaagct
    25921 tataaggtat ttaaagctgc tgaagaaaac aatactctca aagttatcat cgaaaacgat
    25981 attactccag cctgataatt tttaaacaaa tgaaaatgtg agaagaatag ccttctcaca
    26041 tttttattta tgactcaagc gacgtgttaa aaaatcacct aaaaattgga taaagaaaat
    26101 taataataaa atcagaatag tcgctactaa agtaatatca tcacgagaat agttatatcc
    26161 tttagtaata gcaacagaac ccaatcctcc tgcaccaata gcgccagcca tagctgtttc
    26221 acctactaaa gaaatcaaag taaccgttga tactcgaatt aaatctggta gaccttcacg
    26281 aagataaact acaataatat cccaaagtgt tccacctgag gcttgtgcag cctcaataac
    26341 tccaccatca agttcagcta aaacaacttg aacttgacga gcaaaaaatg ggaaaactgc
    26401 caaagaaaga ggtaccaaag ctgctggtga accaagtgtt gttcctacaa ttacgcgagt
    26461 tactggcgca atcaaagcaa gaagaataat gaagggcaga gctctaaaaa cagaaacaac
    26521 tttatctaaa actccaaata ctaatttatt agcaataact cctctaggac tagttaaaac
    26581 aaggaataaa cctcctaaca atcccattaa acctccaata aggaacgacc aaaaagtcat
    26641 ataaagggtt tgtacaatag ctgtctgcca gccgtaagca ccttcccaac ccatttgata
    26701 tacatttggt aaatgtgttt gaatccactc tatcatgctt taccaccttc tttcaataac
    26761 gttaattgaa cttgcgaatc tgtaattgct tggcaggctt gtcttaattt ttcttcgtca
    26821 cctgataaga taactaccat ttctccaact ggaataccat ctaaaatttc aatattccca
    26881 tagaggatgt tagcagttac ttcaaattct ttataaattt gatttaaaag tggctcatcc
    26941 gtgctatgcc cagcgtattt cagttgaact aatttgctac ctactggtag atttttgaca
    27001 acttcttgtt gctcaatctt aagcattgcc tcatcaatcc cagtagctat ttttataaaa
    27061 tcttgtgtaa gactctctct aggatgtgaa aaaatgtcta aaactgatcc ttcttcaatc
    27121 aatttaccat tttgcataac agcaacgcga ttagcaatgt ctttgacaat ttgcatctca
    27181 tgtgtaatca aaacaatcgt aagtcccaat tttttattca gatcttggag aagggcaagt
    27241 atttgttttg ttgttttagg gtcaagtgca gaggtagact catctgaaat taagatttta
    27301 ggatcgttag ccaatgcacg cgcaatggca acacgctgtt tttgccctcc tgataattgt
    27361 gaaggatagt tttgagcacg atcggataat ccaacaagtt ctaataattt agcaacctta
    27421 gctgctttcg cctcttttga taaacctgaa tgtttaagag caaaagctac attctgttct
    27481 gctgtcaact gtgacattag gttaaaatgt tgaaaaatca ttccaatttc acggcgctgt
    27541 tcgcgtaatt gtgtgctagt cagtgtcacc ttgttgtcgt aaataacttg gtcatcaata
    27601 gtaattttac ctgctgaagg ttcttgtagc aaattaataa cacgaacaag ggtagatttt
    27661 ccggctcctg aataaccgac aattccatat atatcacctt gattaatatg gatagtaaca
    27721 tctttaacag cattaatctc acgtttcttt tggtggaaag taacatcaat gttgtctaat
    27781 ttaataattg gttctttact catatgattc tattaaagcc tccactaatt caatatgggt
    27841 gtaataatca gcaatactaa cattttcatc aacgccatgg tctcgactat tggcatgtcc
    27901 tatgccaata gctgcaattg gcgctcttag agcttgatga actaaataca ttggtcctgt
    27961 acctggtgag gtaggtaaca gagatatccc ttctggataa aattgctcag ctaaatcaac
    28021 aacctttagg atagatggcg ctgacatatc actacgataa gacatttcac caagggtata
    28081 ggttagctca atgtctttaa agccattctc tttcaaatga ttttgtattg aatctagtac
    28141 acctttcggg gttaaccctg gtactaacct aacctctgct ttacatttag cataagcagg
    28201 taaaattgtt ttgacgcctt caccttgata acctgaggtt ataccttcaa tggctataga
    28261 aggttcaaaa taaagctttc tcaaaaaagt tttatgacta tctgctaaac tgggcaaaac
    28321 taattggtaa gcaccttcta gcgcctttgc agatctataa gaatattttt caactaattc
    28381 taactctctc ttagtagggg gaataacttt atcataaata ccttctacca atatatgacc
    28441 tttgttatcc cttaacgtat tgagggcttg aattaaatac catgtcgagg aatcaacaac
    28501 cccaccaaag gatgaatgaa tatcaacatc cgctgaactt actgataaat caaaagtgac
    28561 aatgccttta tttccaccag caatttccaa ctgtccttta ggatttttag ggccatcttc
    28621 ccacacgatc aaatcagccc cttgtagctg ctcttgatac ttttctaaat aataatcaag
    28681 gcctactgaa gctgattcct ctgctccttc cacaataaaa gtgatatcta gaggaagttc
    28741 ccctttgtgt cttgataaat attttttaac tgctgataaa cgtgcggtaa tgtgaccttt
    28801 atcatcatct acaccacgtc catacatctt accatatcga agtgaaagtg taaaagggtc
    28861 ctcagtccac tgttctacct catctgctgg tacagtatca taatgattgt aaaaaataat
    28921 acgcttagca tctacttttg atgatttgaa atttgccacg ataaagggag cgctatagct
    28981 gtcatctata atgacttctg cgccagcttt tctgaaaact tctccaagat agcttgataa
    29041 gtcatttaaa ccaactttct gagcaaaaat tgacttaatt gcaattaaat ctcttaaaat
    29101 attgatatca tcctgaacaa tagtatcaga tagaaacttt tgaatttgat tttcttcgtt
    29161 tgttaaagtc atctttcatc caccttcctt caaaaacaat ccaagagcct ggcacattgt
    29221 attcgtccca ggccctttat ggctatatgg ctgataagtt gtatcctatt aggattccct
    29281 gttaaactta tacagtcaat tcacgttacc attgtggaat atcagctgaa gtatctttga
    29341 taactttttt cacttcatct gtgtgataag catccaagat agcttggata gctttagcgt
    29401 tcttttgctt tttccaattt ttacgtcccg caatgatatt aatccattgt tttgaatttt
    29461 tatctgattt ctcaacaaag atagcatctg aaggttttaa attagcttgc tcaatgtatg
    29521 tattattaat aatagctgca tctacatctt tgagtgcacg tggtgtttga ctcgcatcta
    29581 actcctgaat attaatatcc tttttattag atgtgatatt agcaactgtt gcaaccttct
    29641 taccagaaac attcaatttg attaaacctg ctgactgaag gacatacaat gcacggctac
    29701 catttgttgc atcatttgga attgcaatag tggctccttt tttcaatttt ttaagagatt
    29761 ttaccttctc agaatagata cgaattggag ctaagtaagt cttttcaagt ggaattaagt
    29821 ttttcttatt ttccttattc cagttttcta agaaattgta atgttgaaag gcattaatat
    29881 ccacatcctt attggctgtc gcttgatttg gttgtgtata atctgtaaat tctgtaaatt
    29941 tgattttagc tttatcacct actagctttt caattttatc ccaacgtgct ttttcagtgt
    30001 cagaaaaggt cataacacca actttaacaa ctttacttga agttgagctt gctgaaactt
    30061 cttgattact aaatgtgact cctaaaagtg ttgttaaggc taatcctaag acaatatgtt
    30121 ttaacttcat aataaattcc ctcaattttt gtgataaaga tattatccca taggaagtta
    30181 ttagaatcaa attgttataa actgtctctt gttatagttt taaactatat aacaaaaaac
    30241 taaggattga catctcctta gtttttatta ctacttctcc ttaggagtaa cgagatcttt
    30301 ctcatttggt acgtaatcac ctccaaggta ttctttagct atttttgaaa gtgtcccatc
    30361 tttacgtaat tgtttaaggc gattattcac atacttttgc aagtcttttt gatcctgacc
    30421 aaagatgaaa taaatataag gctgatctct catagtcaaa gggatggttt ttaaattcgt
    30481 gagaccttga ttctttatga tagcgttaac agttggtccg tcaaaaagtt taaaatcagc
    30541 ttttccatca ctcaaattcg ttagaatctg tgtaatattt tcattagtat attttagagt
    30601 aacaggattt ttctgatgct ctttattgaa attttctaat tgcttggcag ttgtcgttcc
    30661 ttggacaacc tgtgttttat gaccactaat atcattataa gctttgatat tactattctt
    30721 aggaactgct aaaactgaag gagtagagcc tattgggtat gaaaataaat atttttgaga
    30781 tctctctgat gaataagaaa tattatttcc acccatttga aactttcctg aatcaatgcc
    30841 ggtaaatacc gatgaccatt ctgttttttt aaaagtgact ttatagttat ctgaaccttt
    30901 aaaaacagct ttggctactt caatatcaaa gccagtcagt ttcccatcct tcacataaga
    30961 aaaaggtgcc gttgttccaa cagttgcaaa attaacttct tttttgcccg tcttagtagt
    31021 atgagatttt gagctacaag cagctagaga cgctactgtc actaaaataa ttcctgttac
    31081 acttaacact ttttttattg tcatcttttt tctcctcttt aattgtacac gacatcataa
    31141 catctttaag tttttaacta atacttatct tctatcgtct gagagttttt gctattatat
    31201 ggatgctacg tttcattaac aaaataacca aataaggttt ggttttcaat ttggtgaagc
    31261 attaattctg gatgccattg aacacctata atacattgtt ctgttcgact ttcataagct
    31321 tctactgtcc catctctagg atctctagca ctaactatca gaccttcagc taacagttta
    31381 acactttgtc tatgaagaga attgatctta aaatcacctg atataaagcc ttctagaaaa
    31441 ctcccttttt ccacatttaa ataatgagca aatccaatgt aaggctcttg atagtgatta
    31501 tcaatagatt gattaagtgt accaccaagc gccacattta ctaactgcat gccacggcaa
    31561 atcgcaaaga ttggtttatt ttgtttcaac gcttcttcga ctaatgcaaa ttcaaaaata
    31621 tcacgcgcaa gtgaataatc atcactctca attatcttct cttcaccata ataggatggt
    31681 aaaacatttt ggccaccaga tatgatcagc ttatctatca tctcaacata agctttggca
    31741 ctttctgctt cacttattgg taggataact ggtaacccgc ctgcgttttt aactccttcc
    31801 gaaatatgcc tagataccga atcataataa taacctggaa tatctgacat ttctctttca
    31861 tttcctgtaa taccaataat aggctttgtt aacattttgc tccctcatca accaataact
    31921 gtctaataat tatcatttta cacctaaaaa aactttttga ataatatttt ttacctatca
    31981 cttctttaac aatacctatc aactctcaca aaaataaaaa ccatagtatt aatactacgg
    32041 ttttattttt tagaaatctg ctacattatg ataaactttt tgaacatcat catctgattc
    32101 gagtgcgtca ataagctttt caaaagtttc aaggtcatca ccttccaata ctacttctga
    32161 ttgaggaatc atttcaagtt cagtaacttg gaattcttct acaccattat cgcgaagtgc
    32221 ttggatacct ttatgaagat ctgttggggc ggtataaact gttattgttc cctcttctgc
    32281 ttcaacatca tctacgtcta catccgcttc aagtaattgt tcgaagacag tgtcagcatc
    32341 atcaccagca aaaacgatga cacctttttt atcaaataag taggataccg atcctgaagc
    32401 tcccatattg ccaccgttct taccgtaagc agtacgtaca tttgctgccg tacggttaac
    32461 atttgatgtc aaagtatcca caataatcat tgaaccattt ggaccaaaac cttcatagcg
    32521 tccctctacg aaagtttcat ctgtgtttcc tttggcttta tcaatcgctt tatcaataac
    32581 atgctttgga acttgtgctt gcttagcacg gtccaaaacg aattttagag ctgagtttga
    32641 ctcggggtct ggttcacctt gctttgcagc aacatatatt tcaacaccga atttagcgta
    32701 tacttttgag ttagcaccat ctttagcagt ctttttggca acaatattgg cccacttacg
    32761 tcccattgga aaatctcctt tgatttcatt tttaataacc tgctggctca tattggcaag
    32821 caatcttaat tttaactcta ttattatagc actttgatag caattggtaa aatggaaatt
    32881 ctatcacggt atgcagtaga attttttaga attgtcaatt ttctattaag gagaacctac
    32941 cgagttcctt ttataaaaag actgaacaaa ttttcctttg cccagccttg acagattcgg
    33001 tgttatcctg tatcttacga taaagactaa gtaagggaac gttgtaattg gacttactcg
    33061 gtaaagcgta gtggttacca taactgcgct tcttt
//
LOCUS       DATROQ010000013        28902 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.013, whole genome shotgun sequence.
ACCESSION   DATROQ010000013 DATROQ010000000
VERSION     DATROQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28902
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.013"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(350..736)
                     /gene="rplQ"
                     /locus_tag="VBC94_000563"
     CDS             complement(350..736)
                     /gene="rplQ"
                     /locus_tag="VBC94_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEO6978858.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEASDKYTSTTALQKLFDDIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(751..1689)
                     /locus_tag="VBC94_000564"
     CDS             complement(751..1689)
                     /locus_tag="VBC94_000564"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEO6978859.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELDVEGP
                     AEITAGDILTDSDIEIVNPDHYLFTIAEGHSLKATMTVAKNRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKTEPEMMKVRNLGRKSLEEVKIKLADLGLGLKNDK"
     gene            complement(1739..2122)
                     /gene="rpsK"
                     /locus_tag="VBC94_000565"
     CDS             complement(1739..2122)
                     /gene="rpsK"
                     /locus_tag="VBC94_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEO6978860.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2140..2505)
                     /gene="rpsM"
                     /locus_tag="VBC94_000566"
     CDS             complement(2140..2505)
                     /gene="rpsM"
                     /locus_tag="VBC94_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEO6978861.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLSTSKKILAAAGISEDIR
                     VKDLTPDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2523..2639)
                     /gene="rpmJ"
                     /locus_tag="VBC94_000567"
     CDS             complement(2523..2639)
                     /gene="rpmJ"
                     /locus_tag="VBC94_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEO6978862.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2665..2883)
                     /gene="infA"
                     /locus_tag="VBC94_000568"
     CDS             complement(2665..2883)
                     /gene="infA"
                     /locus_tag="VBC94_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEO6978863.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(2999..3637)
                     /locus_tag="VBC94_000569"
     CDS             complement(2999..3637)
                     /locus_tag="VBC94_000569"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEO6978864.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTQ
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPTCLIERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPILEHYRKLGLVTDIEGNQEITEVFADVEKALLELK"
     gene            complement(3732..5036)
                     /gene="secY"
                     /locus_tag="VBC94_000570"
     CDS             complement(3732..5036)
                     /gene="secY"
                     /locus_tag="VBC94_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEO6978865.1"
                     /translation="MFLKLLRDALKVKMVRNKILFTIFILLVFRIGTHITVPGINVKS
                     LEQMGELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLFLAFVQSIGITAGFNTLSSVALVKTPNVQTYLLIGAILTTG
                     SMVVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAITTIYEDFFVNVRSSAITNSYIF
                     VGILIVAVLAIVFFTTFIQQAEYKIPIQYTKLVQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFFQNGKEIPWLTKLQELLNYQTPVGMIIYAILIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSIRPGRETEEYMSSLLKKLATIGSVFLAFISLLPIIAQQALHL
                     SSSIALGGTSLLILIATGIEGMKQLEGYLLKRRYVGFMNTTE"
     gene            complement(5057..5497)
                     /gene="rplO"
                     /locus_tag="VBC94_000571"
     CDS             complement(5057..5497)
                     /gene="rplO"
                     /locus_tag="VBC94_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEO6978866.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRMPKRGFSNINAKEYALVNLDQLNVFEDGTEVTPVVLKEAGIV
                     RAEKSGVKILGNGELTKKLSVKAAKFSKSAEAAITAKGGSIEVI"
     gene            complement(5622..5801)
                     /gene="rpmD"
                     /locus_tag="VBC94_000572"
     CDS             complement(5622..5801)
                     /gene="rpmD"
                     /locus_tag="VBC94_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEO6978867.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVNAISHLVTVEEA"
     gene            complement(5816..6310)
                     /gene="rpsE"
                     /locus_tag="VBC94_000573"
     CDS             complement(5816..6310)
                     /gene="rpsE"
                     /locus_tag="VBC94_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEO6978868.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDRNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMVEVPMVGTTIPHEVRSEFGGAKVLLKPAVEGA
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6329..6685)
                     /gene="rplR"
                     /locus_tag="VBC94_000574"
     CDS             complement(6329..6685)
                     /gene="rplR"
                     /locus_tag="VBC94_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEO6978869.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNIFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSNGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADSARENGLKF"
     gene            complement(6786..7322)
                     /gene="rplF"
                     /locus_tag="VBC94_000575"
     CDS             complement(6786..7322)
                     /gene="rplF"
                     /locus_tag="VBC94_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEO6978870.1"
                     /translation="MSRIGNKVITLPAGVEIINKDNVVTVKGPKGELTREFNKNIGIT
                     VEGTEVTVTRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKALEMRGVGYRAQLQGSK
                     LVLSVGKSHQDEVEAPEGVTFEVPTPTTINVIGINKESVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(7432..7830)
                     /gene="rpsH"
                     /locus_tag="VBC94_000576"
     CDS             complement(7432..7830)
                     /gene="rpsH"
                     /locus_tag="VBC94_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEO6978871.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIADILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGQNGERVITNLKRISKPGLRVYTKHEDMPKVLNGLG
                     IAIVSTSEGLLTDKEARQKNVGGEVLAYIW"
     gene            complement(7985..8170)
                     /locus_tag="VBC94_000577"
     CDS             complement(7985..8170)
                     /locus_tag="VBC94_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEO6978872.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCEKCGRPHSVYRKFQLCRVCF
                     RDLAYKGQVPGVTKASW"
     gene            complement(8188..8730)
                     /gene="rplE"
                     /locus_tag="VBC94_000578"
     CDS             complement(8188..8730)
                     /gene="rplE"
                     /locus_tag="VBC94_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEO6978873.1"
                     /translation="MANRLKEKYTNEVVPALTEKFNYSSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(8754..9059)
                     /gene="rplX"
                     /locus_tag="VBC94_000579"
     CDS             complement(8754..9059)
                     /gene="rplX"
                     /locus_tag="VBC94_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEO6978874.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVVVEGVALIKKH
                     QKPNNENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYNKKSGEVLD"
     gene            complement(9139..9507)
                     /gene="rplN"
                     /locus_tag="VBC94_000580"
     CDS             complement(9139..9507)
                     /gene="rplN"
                     /locus_tag="VBC94_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEO6978875.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9532..9792)
                     /gene="rpsQ"
                     /locus_tag="VBC94_000581"
     CDS             complement(9532..9792)
                     /gene="rpsQ"
                     /locus_tag="VBC94_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEO6978876.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(9818..10024)
                     /locus_tag="VBC94_000582"
     CDS             complement(9818..10024)
                     /locus_tag="VBC94_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEO6978877.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(10034..10447)
                     /gene="rplP"
                     /locus_tag="VBC94_000583"
     CDS             complement(10034..10447)
                     /gene="rplP"
                     /locus_tag="VBC94_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEO6978878.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGTPEGWVAPVKRGKV
                     MFEIAGVSEEVAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10451..11104)
                     /gene="rpsC"
                     /locus_tag="VBC94_000584"
     CDS             complement(10451..11104)
                     /gene="rpsC"
                     /locus_tag="VBC94_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEO6978879.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11117..11461)
                     /gene="rplV"
                     /locus_tag="VBC94_000585"
     CDS             complement(11117..11461)
                     /gene="rplV"
                     /locus_tag="VBC94_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEO6978880.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKNVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11477..11755)
                     /gene="rpsS"
                     /locus_tag="VBC94_000586"
     CDS             complement(11477..11755)
                     /gene="rpsS"
                     /locus_tag="VBC94_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEO6978881.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(11854..12687)
                     /gene="rplB"
                     /locus_tag="VBC94_000587"
     CDS             complement(11854..12687)
                     /gene="rplB"
                     /locus_tag="VBC94_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEO6978882.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTNTPEKSLLVSLKNKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YILAPKGLEVGQRIISGPEADIKVGNALPLANIPVGTVIHNIELQPGKGAELIRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCSATIGTVGNEQQSLVNIGKAGRNRWKGVRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQ
                     K"
     gene            complement(12705..13001)
                     /locus_tag="VBC94_000588"
     CDS             complement(12705..13001)
                     /locus_tag="VBC94_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEO6978883.1"
                     /translation="MNLYDVIKKPVITEKSMVALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVTVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(13001..13624)
                     /gene="rplD"
                     /locus_tag="VBC94_000589"
     CDS             complement(13001..13624)
                     /gene="rplD"
                     /locus_tag="VBC94_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEO6978884.1"
                     /translation="MANVKLFDQTGKEVSSVELNEAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVENLSFAAPKTAEFASVLSALSIDSKVLVILEEGN
                     EFAALSARNLPNVTVATATTASVLDIVNADKLLVTKEAISTIEGVLA"
     gene            complement(13648..14274)
                     /gene="rplC"
                     /locus_tag="VBC94_000590"
     CDS             complement(13648..14274)
                     /gene="rplC"
                     /locus_tag="VBC94_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEO6978885.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVVLIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14347..14655)
                     /gene="rpsJ"
                     /locus_tag="VBC94_000591"
     CDS             complement(14347..14655)
                     /gene="rpsJ"
                     /locus_tag="VBC94_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEO6978886.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(14862..16142)
                     /locus_tag="VBC94_000592"
     CDS             complement(14862..16142)
                     /locus_tag="VBC94_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO6978887.1"
                     /translation="MRYSKEIIQLAIPAMIENILQMLMGVVDNYLVAQLGVVAVSGVS
                     VANNIITIYQAIFIALGASIASLLAKSLAGSEKDDAISVCSQAIFLTSLIGAVLGIIS
                     IVFGQTFFKLLGTTKSVAQVGGLYLAIVGGGVVTLGILTTLGSFLRVQGQPRLPMYVS
                     IFVNFLNAVLSGFAIFEWRYGLVGVAVSTLIARLIGICILAKYLPIKKIIKRMTWKIS
                     AQIWNLALPSAGERLMMRAGDVVIVAIVVQLGTNVVAGNAIGETLTQFNYMPGLGIAT
                     ATIILTAKYVGQKNRESIEETIQSSYYIGLVLMILISSFMLLAGKPLTQLFTNNPSAI
                     KGSLIVILLSFVGVPATIGTLVYTAAWQGLGNAKLPFYTTTIGMWLIRVVLGYLLGIV
                     FELGLLGVWMATIADNIFRWLFLKVRYHRYIQKM"
     gene            complement(16247..17737)
                     /locus_tag="VBC94_000593"
     CDS             complement(16247..17737)
                     /locus_tag="VBC94_000593"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEO6978888.1"
                     /translation="MAIVYQSTRDASNKVTASQAILKGLADDGGLFTPITFPKVDLDF
                     TKLKDASYQEVAKLVLSAFFDDFTEQELDYCISQAYDTKFDTTEIAPIVKIGDRYHLE
                     LFHGPTIAFKDMALSILPYLLTTAAKKQGVDDKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKHGVSYIQELQMITQAGQNTHVVAIEGNFDDAQTSVKEMFNNSLLRSKLSQ
                     QHMQLSSANSMNIGRLVPQIVYYIYAYAQLVKSKEISIGQLINFSVPTGNFGNILAAY
                     YASQIGLPVTKLICASNDNNVLTDFFKTQTYDKNREFKVTSSPSMDILVSSNLERLIF
                     HLLGDDAETTKKLMEDLVTTGEYALEARQANILESFVAGFATEQFVELDIKRLFDQYQ
                     YIEDPHTAVASAVYQAYQTETKDQTPTVIVSTASPYKFPCVVTKAITNKEEIQDFAAI
                     SILNDLSGVSLPKAVTDLQKAEVIHRMVVPTSNMRETVEECLGLKQ"
     gene            complement(17857..18873)
                     /gene="adhP"
                     /locus_tag="VBC94_000594"
     CDS             complement(17857..18873)
                     /gene="adhP"
                     /locus_tag="VBC94_000594"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEO6978889.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPKVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLRVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAIVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIAVYGAGGLGNLAVQYAKKVFNAHVVAVDINADKLQLAKEVGADLTVNGKEIK
                     DVAAYIQEKTGGCHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIRVVGSLVGTRKDLEEAFAFGAEGLVVPVVEKVPVDTAPQVFDEMERGLIQGRKVL
                     DFTK"
     gene            complement(19051..21693)
                     /gene="adhE"
                     /locus_tag="VBC94_000595"
                     /gene_synonym="adhC"
     CDS             complement(19051..21693)
                     /gene="adhE"
                     /locus_tag="VBC94_000595"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEO6978890.1"
                     /translation="MTEKTKAVETTDVALAIDTLVQNGLKALDEMRQLNQEQVDYIVA
                     KASVAALDAHGELALHAVEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEEDDV
                     TGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWIEQPSIDATNALMKHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYKEFVEEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKSAVWIAEQAGFTVPEGTN
                     ILAAECTEVSEKEPLTREKLSPVIAVLKAESTEDGVEKARQMVEFNGLGHSAAIHTKD
                     ADLAREFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTDLMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVMTVPGFIAADTGMDVLTHATEAYVSQMANDY
                     TDGLALQAIKIVFDYLERSVKDADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPAATP
                     EEAVESYAKAVYDLGTRLGIKMNFRDQGIDEKEWKEKSRELAFLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYEERPGRRK"
     gene            complement(21962..23740)
                     /locus_tag="VBC94_000596"
     CDS             complement(21962..23740)
                     /locus_tag="VBC94_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO6978891.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFKNSFPGGFVGVDIFFTFSGFL
                     ITALLIDEFSKTKKIDFVSFCRRRFYRIFPPLVLMVLVTIPFVFLVKSDFRASIGSQI
                     MTALGFTSNFYEILTGGNYESQFIPHLFVHTWSLSIEVHFYVLWGLTVWLLSKRSKDQ
                     KQLRGTLFLVSMGVFGVSFLTMFVRAFFVDNFSTIYFSTLSHIFPFFLGAMVATISGI
                     REITGRFKKNIKNLTLKHNLIMMGSAFAGLMILTFALDFDNRLTYLFGFVLSSIFASV
                     MIYNARILHEHTPDISEPFVITYLADISYGMYLFHWPFYIIFSRLSPNWIAVILTVVL
                     SAVFSTLSFYIIEPFILGRKPKFLDYEFDLLPYKKWLFSIGGVLTLITVVTMLTAPSI
                     GSFETELLQNSLQQARTNTNRTHTLAAGDAGALSDVTVIGDSVALRSSAAFNKLLPEV
                     QLDAAVSRNFSKSFDIFENRIQNKALSKIVVLAVGVNSLDNYKTDLSQFIKSLPKGHR
                     LIIVTPYNAKNMSQVTTVRDYELSLMKKYNYITVADWYKVATEHPEIWGNTDGVHYSD
                     SDTTGADLYVSNVKKAIQKSAQRAAK"
     gene            complement(23737..24117)
                     /locus_tag="VBC94_000597"
     CDS             complement(23737..24117)
                     /locus_tag="VBC94_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978892.1"
                     /translation="MKNFKITRTHLEILSLIIIVVFGLSVFTLTTSSQGVLSYDGGKI
                     KYVGSIVNHHMTGKGKLTYENGDYYKGDFVNGVFEGKGTFVSVHGWSYTGDFKKGQPD
                     GQGRLNAKNKKVYKGTFKQGIYQK"
     gene            complement(24124..24561)
                     /locus_tag="VBC94_000598"
     CDS             complement(24124..24561)
                     /locus_tag="VBC94_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEO6978893.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMANLDKEDSLSVESRATSTWEHG
                     NPIHRGTQAILQRYQIPYTNDKGSQPISKDDLAYFDYIIGMDEQNVKDLKALSQGTYD
                     DKISLFCDGGVPDPWYTGDFEETYRLVSDGCKQWLTLINNSRD"
     gene            complement(24713..25711)
                     /gene="ruvB"
                     /locus_tag="VBC94_000599"
     CDS             complement(24713..25711)
                     /gene="ruvB"
                     /locus_tag="VBC94_000599"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001902090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEO6978894.1"
                     /translation="MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEA
                     AKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAIEKSGDLVAILNDLE
                     PGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPR
                     IANRLLKRVRDYAQIMGDGLIDDNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGG
                     PVGLGTLSVNITEERDTVEDMYEPYLIQKGFIMRTRTGRVATDKAYEHLGYQRFDK"
     gene            complement(26008..26919)
                     /locus_tag="VBC94_000600"
     CDS             complement(26008..26919)
                     /locus_tag="VBC94_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6978895.1"
                     /translation="MLKHFGSKVRNLRVTRNITREDFCGDETELSVRQLARIESGQSI
                     PNLTKAHYIAKQLNVKLDILTGGESLELPKRYKELKYLILRIPTYADAERLKLRECQF
                     DHIFEEFYDNLPEDECLAIDSLQAKFEVYQTGDINFGVEVLCECFDKVKYKEKYTLND
                     LIIIDLFLTCAVVSKFNNRAFTKEVFQTICKTLISQNHKLTAEDLFWFNHVLLNCVFV
                     GLCLNSEECLAEMLEVSRQTMVSTHDFHKMPLYFMYQWKYFITIDNDIKSAENAYQQS
                     IMFSKMIDDKHLIKKLELEWQEDITGH"
     gene            complement(27072..28370)
                     /gene="purB"
                     /locus_tag="VBC94_000601"
     CDS             complement(27072..28370)
                     /gene="purB"
                     /locus_tag="VBC94_000601"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEO6978896.1"
                     /translation="MISRYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEKYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEEIDELFNPIYYTKRVDDIFERLGLEK"
     gene            complement(28388..28902)
                     /locus_tag="VBC94_000602"
                     /pseudo
     CDS             complement(28388..28902)
                     /locus_tag="VBC94_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613836.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8713 a         5984 c         4868 g         9337 t
ORIGIN      
        1 attatactaa atatttctac ttcctgtcaa tactattttt gtattttata aatttagtat
       61 agacataact attcctctat attcaattaa gagaaattat ataaccacta ttgagaaatg
      121 tagtctagct attaaactct ttgctcatcg aaaatatcca ataaatataa tacataaaac
      181 gcctgcttac gaaatataaa caaaattgtt tgcatttcgt aaacaagcgt tacctattta
      241 acaatatatg atgagtgttc ccgctgagaa taattctcag cggtagacca gagctagact
      301 aagaatcgat tgattccatc atcataacac tcaacaaaat tgataaaaat tatactaatt
      361 caataattgc cattggggca gcatcgccac ggcgtggttc tgttttaaga atacgagtgt
      421 atcctccgtt acgttcagca taacgaggtg cgatgtcgtc aaaaagtttt tgaagagctg
      481 ttgttgatgt atatttatca gaagcttcat catagttttc tgatgcaatt tcattacgta
      541 cataagcagc agcttgacga cgagcatgta aatcaccacg tttacctaga gtaatcattt
      601 tttcaactgt tttacggatt tctttagcac gtgcttcagt agttacaatt gattcgttga
      661 taagaagatc ggttgtcaaa tcacgaagca ttgccttacg ttgtgagcta gtgcgtccta
      721 gtttacggta agccattatg tcctcctatt ttatttatcg ttttttaatc caagacctag
      781 atcggcaagt ttgattttaa cttcttcaag actcttacgt cctaagtttc ggactttcat
      841 catttcaggc tcagtttttt ctgttaaatc aaatactgta ttaattccag cacgttttaa
      901 acagttatat gagcgcactg acaaatcaag ttcctcaatt gtgcggtcaa gcactttctc
      961 atcgttcact ttctctgttt ccttcattac ttcagttgct ttagcaacct ctgttaaatc
     1021 agtaaacaag tttaagtgtt caattaagac gcgagctgaa agaccaagag catcctcagg
     1081 aatgattgtg ccatttgtca tgatttcaat agttaattta tcaaagccat cattactacc
     1141 tacacgagca ggttcaactt gataattaac ttttttcact ggtgtataga ttgaatctac
     1201 agccaatgtt cctactggtg catcatcttt tttatttcct tctgctggaa cataacctcg
     1261 gtttttagca acggtcattg ttgctttcaa actgtgacct tcagcaattg tgaaaagata
     1321 atggtcaggg tttacaattt cgatatcact atctgttaaa atatctccgg cagtaatttc
     1381 tgcaggacct tcaacgtcta gttcgataat cttttcgtct tcgacgtaag atttcacagc
     1441 aagtcctttt acattaagga tgatttgcat cacatcttca cgtacacctg ggatagtatc
     1501 aaattcgtgt aatactccat caattttgat tgatgtgact gctgcacctg gaagtgaaga
     1561 cagaagcaca cgacgaagag agtttcctag agttgtaccg tagccacgtt ctagtggttc
     1621 gatgacaaat ctgccgtaat ctttattttc atcaattttt gttattattg gtttttcaaa
     1681 ctcaatcatt tagttactcc ctcttaaacg aaaagttgtg tactataaaa tgctatgatt
     1741 atacacgacg acgttttgga ggacgagcac cattatgtgg tacaggagtc acatcacgaa
     1801 ttgcagttac ttcaagacca gctgcagcaa gggcacggat agcagattca cgacctgaac
     1861 caggaccttt tacagtaact tcaacagttt taagaccgtg ttcttgtgca gatttagcag
     1921 cagcttcagc agccatttga gcagcaaatg gagtagattt acgagaacct ttaaatccaa
     1981 gagcaccagc tgatgaccat gcaagagcgt taccatgcac atctgtaatc ataacaatag
     2041 tgttattgaa tgtagcgtga atatgagcaa caccagattc gatgttcttt ttcacacgac
     2101 gtttgcgtgt tggtttagcc aattgtttta cctcctattt tattttttct tacctgcaat
     2161 cgcaacagct ttacctttac gagtgcgagc gttattttta gtattttgtc cacggacagg
     2221 aagtccacga cggtgacgga ttccacggta tgaaccgatt tccatcaaac gtttaatatt
     2281 caagtttacc tcacgacgta ggtcaccttc aactttgatt gcatcaactt cacgacggat
     2341 agcgtcctct tgatctggtg tcaaatcttt aacgcggata tcttcagaaa tacctgcagc
     2401 tgctaagatt tttttagatg tagaaagacc aattccgtat acataagtaa gtgaaattac
     2461 tacacgtttg tcatttggaa tatcaactcc agcaatacga gccattttat ctcctttcta
     2521 tattatcctt gacgttgttt gtgttttgga tttgtcggac aaattaccat aacacgaccg
     2581 ttacgacgaa taactttaca gtattcgcaa attggtttaa ccgatggtct taccttcatg
     2641 ttttaatccc tccaattatt tcgactattt aaagcggtat gtgatgcgtc cacgtgttaa
     2701 atcgtatggg ctcatttcaa cagtaacacg atcacctacc aaaatacgga tgtagttttt
     2761 acggattttt cctgatacag ttgccaaaat ttggtgtcca ttttccaact caacagtaaa
     2821 cattgcgtta ggcatagttt caacaacttt accttcaatt tcaatcacat cttcttttgc
     2881 cacgcaaaag taccccctaa atttcgattt gatgtcctct ataaagaggt aacaattata
     2941 agtcagacta acttagtata tcattactaa tctacttatg caagtagatt agcataattt
     3001 atttgagttc tagcaacgct ttttcaacat ctgcaaaaac ttctgttatt tcttgattac
     3061 cttcaatatc tgtaacaaga ccaagcttac gatagtgttc aagaataggt tctccttgag
     3121 caatattaac gtccaagcga cgtttgacag tttcaggctt atcatcttca cgttgatagt
     3181 aatcttcttc tttataatct actggtgggt tgaacacttt gtggaaagtt tcaccagttt
     3241 tacgattgat aatacggcca ctcaaacgct ctataaggca tgttggatcc actttaatat
     3301 taataacacc atctaagcgt agtcctagtt cttcaagcgt agcatctaag gcgtgtgctt
     3361 gctcaatagt acgtggatat ccatcaagta aaaaaccttt ttctgcgata tcatcctcag
     3421 ctaagcgctc ttttacaatc ccgtttgtta cttcatcagg aaccaattca cctttatcaa
     3481 tataactttt agctaaacgt cccatttggg tttgattagc cattgcggcg cggaacatat
     3541 cccctgttga gatgtgagca acaccaaatt cttcaacaat cttagctgct tgagtacctt
     3601 taccagcacc aggcaaaccc ataattaaaa gattcattat agtctcctta ttttgttttt
     3661 aaataaatcc aacacagcat gtcggactta ttaaaaaaca aaatagaagg ttgacagagt
     3721 caacctatat tctattctgt tgtattcatg aatccaacat agcgtctctt caaaagatag
     3781 ccctcaagtt gtttcatacc ttcaatacct gttgcaatca aaatgagtaa gcttgtacca
     3841 cctaacgcaa tacttgatga caaatgcaac gcttgttgag caataattgg taacaaggaa
     3901 ataaatgcta gaaatactga accaattgtt gctaattttt tcaatagcga tgacatatac
     3961 tcttctgttt ctcgtcctgg tcgaatactt ggaatgtagg acgaattttt ttgaagattc
     4021 tctgctgtct tttcagggtt tacttgtaca aaagtataga agaaagagaa cagaataatc
     4081 aagatagcat agataatcat accgactggg gtttgataat taagtaattc ttggagttta
     4141 gtaagccaag gaatttcttt accattttga aagaaaggaa taatcgtact tggaattgtt
     4201 gtaatcgagc tagcaaagat aacgggaata acaccagctg gatttacttt tagaggaaga
     4261 tatgaacttg taggagctcc ttgaacaagt tttgtatatt ggattggaat tttgtattca
     4321 gcttgttgaa taaatgttgt aaaaaataca attgctaata ctgctacaat aagaatcccg
     4381 acaaagatgt aagaatttgt aattgctgaa gagcgtacat ttacaaagaa atcttcgtag
     4441 atagttgtaa ttgcacttgg aatggaagaa atgatccccg caaagataat catagacact
     4501 ccatttccga atcccttatc agtaatttgt tcacctagcc aagtaaccac catactacct
     4561 gttgtaagga ttgcaccaat aagcaaatat gtttgtacat tcggagtttt aactaaagca
     4621 acacttgata gagtgttaaa accagcagta ataccaattg attgtacgaa tgcaaggaac
     4681 aaagaaatat aacgtgtagc ttggttaagc ttacgacgac ctacctctcc ttgttttccc
     4741 cattcaacaa acttcggaag aatatccatt tgaagtagct gaacaacaat agacgcagta
     4801 atgtatgggc taactcccat agagaatacc gaaaagtttc tcattgcatt accactaact
     4861 aagttaagca tatttagaaa tggaagttca cccatttgtt ctaaactctt aacattgatg
     4921 cccggtactg tgatatgcgt tccgatacga aatacaagaa ggataaagat agtaaataaa
     4981 attttatttc ttaccatctt tactttgagt gcatctctaa gtaattttaa aaacataatg
     5041 agttacctct cattaattag atgacttcga ttgaaccacc tttagcagtg atagctgctt
     5101 cagcagattt agaaaattta gctgctttaa cagacaattt cttggttaat tcaccattac
     5161 caagaatttt aacgcctgat ttttcagcac gcacgattcc agcttcttta agaacaactg
     5221 gagttacttc agtaccatct tcaaagacat ttaattggtc aaggttcaca agagcatatt
     5281 cttttgcatt gatgtttgag aacccacgtt ttggcatacg acggaacaat ggagtttgtc
     5341 caccttcaaa accaaggcgt actccaccac cactacgcgc tttttgtcct ttttgaccac
     5401 gtccagaagt tttaccatta cctgaagatg taccgcgacc cacacgattg cggactttac
     5461 gtgatccttc tgcaggtttt aattcatgaa gtttcattat tttttctcct tatttgtaaa
     5521 atgctaacgc ctttacgagg ggaaaggtac tccccgcaaa gactcgccta tacgatttgt
     5581 ttaaatcgca ccaataacaa tcagtgcgat ttagctttta attaagcttc ttcaacagtt
     5641 actaagtgag agatagcgtt aaccatacca cggatggcag cattatcttc cttaacaact
     5701 gaagagttta atttaccaag tccaagggca acaactgttt tacgttgttc tggtttgcga
     5761 ccgattggag acttagtcaa agtaatttta atttgagcca ttataatccc ctttcttatg
     5821 ctaagtcaga aacagaaatg ccacgtaaag cagcaacttc ttctgcacgt tttaattgtt
     5881 tcaagccctc aacagttgcg cgaacaatgt tgattggagt gtttgaacca agtgatttag
     5941 atgtaatatc tgcaacacct gctaattcga tgacggcacg aacagcacca ccagcggcaa
     6001 ccccagcacc ctcaacagct ggtttcaaca atactttcgc tccaccaaat tctgaacgaa
     6061 cttcgtgagg aattgttgta ccgaccatag gaacttcaac catgtttttc ttagcagctt
     6121 caacagcttt gcgaatagct tctggtactt cttgagcttt acctgtacca aaaccaacac
     6181 gaccattacg gtcaccaaca actacaagag ctgcaaaacg tagacgacgt ccacctttaa
     6241 caacttttgt aacgcggtta atcgcaacaa cgcgctcttc aagttctact gcattatctt
     6301 taaatgccat tattaagtgt cctccctatt agaatttcaa tccgttttca cgagctgagt
     6361 cagccaaagc ttttacacgt ccgtgataga gatatccacc gcggtcaaac accacttcag
     6421 aaataccttt agctactgcg cgttcagcaa caagtttacc gacaacaacg gcttgttctg
     6481 tttttgttcc gttagaaact tctttatcaa gagttgatgc acttgcgagc gttacacccg
     6541 ctacgtcatc aattacttga gcgtagatgc ctgtattaga acggaaaatg ttcaaacgtg
     6601 ggcgatcagc agttccagag agtttaccgc gaacgcgacg gtggcgtttt tggcggattt
     6661 tatttttatc tggtttcgaa atcacaattt tcacctctta aatatattgt tatgtcaaaa
     6721 tgacaatatt aaaatgaaaa gatagagaat ttgcagaaaa gattctacaa aatctatcaa
     6781 tcctattatt tacctgtttt accttcttta cggcgtacga attcaccaac gtaacgaata
     6841 cctttacctt tatatggttc tggtgaacgt aagctacgaa cgtaagcagc agtttgtcca
     6901 acgctttctt tgttgatacc gattacgttg attgttgttg gagttggcac ttcaaaagta
     6961 acaccttctg gagcttcaac ttcgtcttgg tgagacttac caacagaaag aacaagtttt
     7021 gatccttgaa gttgtgcacg gtaaccaaca ccgcgcattt caagagcttt tttgaaacct
     7081 tcagaaacac caacgaccat gttgttcaag ttcgcacgag ttgtaccatg aattgttttc
     7141 atttcttttg agtcgttagg acgagtaact gttacttcag tcccttcaac agtgattcca
     7201 atatttttat taaactcacg agtgagttcg cctttaggtc cttttacagt aacaacatta
     7261 tccttgttaa tgatttcaac acctgcaggc aatgtaatta ctttattacc aatacgtgac
     7321 atgtttattt tctcctgtta aattgtcaag ctgcttgagc aactagtttt cacggggact
     7381 catgagacca caggattata ctgtgactca atgagcattt taggtaaaat tttaccaaat
     7441 atatgcaagt acttctccac caacattttt ttggcgagct tctttgtcag tcaaaagacc
     7501 ttcagaagtt gatacaattg caataccaag tccgttaaga actttaggca tatcttcatg
     7561 tttagtataa acacgaagac ctggttttga aatacgtttc aagttagtga taacacgttc
     7621 accattttgt ccatatttaa ggaacacacg gatgatgcct tgtttgtcat cttcaataac
     7681 ttcaacgttt tttacaaaac cttcacgttt aaggatatca gcaatccctt ttttaatgtt
     7741 tgatgcaggt acttctaaca cttcatgttt tgcttggtta gcattacgaa tacgagttaa
     7801 aaagtctgca attgggtcag tcataaccat tttttaattc tcctcttatt agcagtttga
     7861 atttaatcac ttgctaatta atgagtcctt acggacgttg taagactagg tcttagtata
     7921 tttgcgttta aaaatcagtg ccattatcaa tgacaccttt taaacacaaa gcatcattaa
     7981 tttattacca agatgcttta gttacgcctg gtacttgacc tttataagcc aagtcacgga
     8041 agcaaacacg gcaaagttgg aatttgcggt aaactgagtg tggacgtccg catttttcac
     8101 aacgagtgta agcttgcgta gagaacttcg ctgggcgttt gttcttagca atcatagatt
     8161 ttttagccaa tttatttacc tcctagatta ctttgcaaaa ggcattccaa ggccttttag
     8221 caattcacgt gactcttcat cagtgttagc agtagtaacg ataacgatat caagaccacg
     8281 tactttatca acgtcatcaa agttgatttc tgggaagatc aattgttctt taactccaag
     8341 agtgtagttt ccacgtccat caaatgattt cgttggaaca ccatggaagt cacgtacacg
     8401 tggaagtgaa acagaaacta atttatccaa gaattcgtac atacgttcac cacggagggt
     8461 aacttttgca ccgatcgcta caccttcacg aagacggaag ccagcgattg atttcttagc
     8521 tttagtaata agtggttttt gacctgaaat aagtgctaac tcagcagcag ctttctcaag
     8581 atttttagcg tttgatacag catcaccaac acccatgtta agaacgattt tctcaacttt
     8641 tggcacagcc ataacagatg aatagttaaa tttttctgtc aacgcaggaa caacttcatt
     8701 agtatatttt tcttttaagc gatttgccat tatgcttctc ctttccttcg tgattaatca
     8761 agcacttcgc ctgatttttt gttgtaacga acttttttgc cgtcaacaac tttgtaacca
     8821 acacgtcctg ctacaccatt tttatcaaga acttgaacgt ttgacacatg gattggagct
     8881 tctttttcaa cgatagcgcc ttgagggttt tcgttatttg gtttttgatg tttcttgatg
     8941 agggctacgc cttcaacaac aactttgttt acttttggaa gagctttaag aactacagct
     9001 tcagtgcctt tgtccttacc agcaataacg cgaactttgt cgcctttttt tacaaacatt
     9061 tgagtttttc tcctattttt cttacgcctc gaaaggcacc ctaggaaatc ctaggggact
     9121 acgtttgtta atctgagatt aaagtacttc tggtgccaat gaaacgatct tcatgtagcc
     9181 accttcacgc aattcacgtg caactgggcc aaagatacga gttccgcgag gagttttatc
     9241 gtcacggatg attacagcag cattgtcgtc aaatttgatg tatgaaccgt ctgggcggcg
     9301 tgcaccagtt tttgtacgaa cgataacagc tttaaccaca tcaccttttt taactgctcc
     9361 accaggagta gcttgtttta cagaagcaac gattacgtca ccgatgttag cgaatttacg
     9421 tcctgaacca ccaagtactt tgatagtcaa gatctcacga gcaccgctat tatcagcaac
     9481 tttcaagcga gtttcttgtt gaatcatttc aattttctcc ttttagtttg attagataat
     9541 aacagctttt tccactactt ccacaagacg gaaacgtttt gtagctgaaa gtgggcgagt
     9601 ttccatgata cgaacgatat cgccttcttt agcaacgttg ttttcatcat gtgctttata
     9661 ttttttagaa tagttgatac gtttaccata gactgggtgg ttacgtttag tttcaactac
     9721 aactgtgatt gttttgtcca tcttgtcaga cactacgcgt ccgtaaagag ttttacgttg
     9781 attacgttcc atttagaatt tctcctttcc caatctatta tttcatttct gattgcacag
     9841 tcttaacacg tgcaatttgt ttcttaactt cgtcaaggcg agcagtcttt tcaagttgac
     9901 ctgctgcagc ttggaaacga agatcaaaca attctttttt gagttcgttt tctttcttag
     9961 caagttcttc ttgagacaat ccacgaagct ctttaacaaa atctttaatt tcttgaagtt
    10021 tcatgtcttc tccttattct gcttcacgtt ttacgaattt gcatttaact ggtaatttgt
    10081 ggctagcaag acgtaatgct tcacgtgcaa cttcttcaga aacaccagca atttcaaaca
    10141 tcactttacc acgtttaact ggtgctaccc aaccttcagg tgtcccttta ccagaaccca
    10201 tacgtacacc gatagctttt gcagtgtatg atttgtgtgg gaagatttta atccaaactt
    10261 taccaccacg tttcatgtaa cgagtcatcg caatacgggc agcttcgatt tgacggtttg
    10321 taatccagtg gctagttgta gcttgaagtc cgtattcacc gaatgaaact tctttaccac
    10381 ctttagcctc accgcgcatt tttccacgga attcgcgacg gtgtttaaca cgtttaggta
    10441 ctaacatttg ttatttgcct cctttagtgt ttttacgagc tggaagaact tctccacggt
    10501 aaatccaaac tttaacgcca agtttaccat aagttgtgtc agcttcttcc caagcatagt
    10561 cgatatcagc acgaagcgtg tgaagtggaa cagttccttc tgaataacct tcagcacgag
    10621 cgatatcagc accgttcaaa cgaccagaaa cttgagtttt aataccttta gctcctgcac
    10681 gcattgtacg ttggattgct tgtttttgag cacgacggaa agcaacacgt tgctctaatt
    10741 gacgagcaat attttcacca acaaggtgag catctaaatc aggttgtttg atttcaatga
    10801 tgttgatgtg tacttgtttt ccagttaatt tgttaagttg accacgaaga gcgtcaacgt
    10861 ttgctccacc tttaccgata accatacctg gtttagcagt gtgaagtgaa acaataactt
    10921 tatttactgc acgttcaatt tcaatagttg aaactgatgc gtcagctaat tctttattaa
    10981 tgaatttacg gattgcaaga tcttcatgaa ggtaatccgc gtattctttt tcagcatacc
    11041 atttcgcatc ccagtcacgg atgattccga cacgcatacc aattggatgt actttttgac
    11101 ccacgatttt acctccttat ttttctgata caactacagt tacgtgagtt gtacgtttgt
    11161 tgattggtga agctgaacct ttcgcacgtg gacggaaacg tttcattgtt ggtccttcgt
    11221 tagcgaaagt ttcagatact accaagtttg ctttttccaa accaaagttg ttttctgcat
    11281 ttgcaattgc tgagttaaga gttttctcaa taacacgagc tgctttgttt ggagtgaatt
    11341 ttaagattgc gattgcgtca gcaacattct taccacggat aagatcaagt actaaacgtg
    11401 ttttacgagg tgaaacacgc actgtacgag ccattgcttt agctgaagta atttctgcca
    11461 ttgtgtcctc ctcctattaa cgacgtgttt tcttgtcgtc agctgcgtga cctttgtaag
    11521 tacgtgttgg tgcaaattca ccaagtttgt gacctaccat gtcctcttgg atataaacag
    11581 gaacatgttt acgtccatca taaactgcga ttgtgtatcc gatgaaactt gggaaaatcg
    11641 ttgaacgacg tgaccaagtt ttgattactt ttttcttttc gtcatttgct tgagcttcaa
    11701 cttttttcat taaatgctcg tcgacgaaag gtcctttttt aagactacgt cccattttgt
    11761 agttttctcc tttaaataat gtaccacaac ggcctgtaca ctatagtgta ctaccgagct
    11821 ggcggatttg tataagatta tgctattaac ctattatttt tggttacgac gacgaacaat
    11881 aagtttgtct gatttagctt tcttattacg agttttaagt ccaagcgcag gtttacccca
    11941 tggagtagat ggtgctttac gaccaactgg tgctttacct tcaccaccac cgtgtgggtg
    12001 atcgttaggg ttcattacag aaccacgaac tgtagggcga acacctttcc aacgattacg
    12061 tcctgcttta ccaatgttaa caagtgattg ctgttcatta ccaacagtac caattgttgc
    12121 actgcaagta ccaaggatca tacgtacttc acctgattga aggcgaacaa gaacatattt
    12181 accttcttga ccaagtactt gagcagaagc accagcggca cgaatcaatt cagcaccttt
    12241 acctggttgc aattcaatat tatggataac tgtaccgact ggaatgttag caagcggaag
    12301 tgcgttacca actttgatgt ctgcttctgg accagaaata atgcgttgac ctacttcaag
    12361 gcctttaggc gcaaggatgt aagctttaac accatcagta tagtgtacaa gagcaatatt
    12421 tgcagtacga tttggatcgt attcaattgt tttaacaact gcttcaacac catctttatt
    12481 acgtttgaag tcaatcaaac ggtaatgacg tttgtgtcca ccaccttggt gacgaacagt
    12541 aatacgtccg ttgttgttac gtcctgcttt attctttagt gaaacaagca atgatttctc
    12601 aggagtgttt gtagtgattt ctgcaaaatc caaagaagtc atattacgac ggccatttgt
    12661 cgttggttta taaactttaa tacccacgtt atttcctcct tagattattc agcttcagca
    12721 gcaaacaact cgattgcttt tgaatcagct gtaagagtga taatagcttt tttagttttt
    12781 gaagtaaaac ctgtgtaacg accaacacgt tttgctttgg gtttaactgt cacagtgttt
    12841 acacttgcaa cttttactcc gtcaaatgca gcttcaacag cttgtttgat caaaagtttg
    12901 tgtgcacgtg tgtcaacttc aaatgtatat ttccctgctt caagagcaac cattgatttc
    12961 tcagtgataa caggtttttt gattacgtca tacaaattca ttatgcaaga accccctcga
    13021 ttgtagagat tgcttcttta gtaacaagaa gtttgtctgc gttaacgata tcaagaacac
    13081 ttgcagtagt tgcagtagca actgtaacgt ttggaaggtt acgcgctgaa agtgcagcaa
    13141 attcgttacc ttcttcaagg attacaagta cttttgaatc aatgctaagt gctgaaagaa
    13201 ctgacgcaaa ttcagcagtt tttggagctg caaatgaaag gttttctaca gctacaaatt
    13261 tatcttcagc aacttttgct gaataaactg atttcaacgc aaggcgacga actttttgtg
    13321 gaagtttgta gccgtatgaa cgtggagttg gtccaaagac aacaccacca ccacgccatt
    13381 gtggtgagcg gatagaacct tgacgagcgc gtccagttcc tttttgacgc catggtttac
    13441 gtccaccacc agatactgct gaacggtttt taaccgcgtg agtaccttgg cgaaggctag
    13501 cgcgttggct gattacaaca tcaaaaacaa ctgattcgtt tggttcgata ccgaagatag
    13561 cttcgttaag ctcaactgag ctaacttctt taccagtttg gtcaaatagt ttaacgtttg
    13621 ccattttaac tgttttctcc tttcttatta tttagcagct ttaactgctg atttgatagt
    13681 gataagagat ttcttagcac ctggtacgtt acctttgatg aggacaacgt ttttctctgg
    13741 gataacttgt acaatttcaa ggttttgaac tgttacacgg ttaccaccca tacgtcctgc
    13801 caagcgtttg tttttgaaaa cgcggttagg cgcaacaggt cccatagaac ctggacggcg
    13861 atggtaacga gaaccgtgag ccataggacc acgtgattga ccatggcgtt tgataacacc
    13921 ttggaaacct ttaccttttg atgtccctgt gacgtcaaca acatcaccag cttcaaattg
    13981 ttctacagat aattctgcac caacttctaa gccttcaatg tttttgaatt cacgaatgaa
    14041 gcgcttagga gctgtgtttg cttttgcaac atggcctttg gcaggtttgt tactcaatac
    14101 ttcacgtttg tcatcaaaac caacctgaac tgcttcgtaa ccgtctgttt caacagtttt
    14161 aacttgaagc acaacgtttg gagttgcttc aatgacagta acagggatga attcgcctga
    14221 ttcagtgaag atttgagtca ttcccacttt tttccctaag attccttttg tcatgagaaa
    14281 atatttcctt ttcttatttt atttcaaaaa gttttttacg agcgtttttt gtgctcagaa
    14341 atcaaattaa agtttgattt ctacgttgac accacttgga agatcaagtt tcatcaaagc
    14401 atcaacagtt ttttgtgttg ggttgatgat gtctaccaaa cgtttgtgtg tacgcatttc
    14461 aaactgttcg cgagaatctt tatatttgtg agtcgcacga ataattgtgt aaagactgcg
    14521 ttcagttgga agcggaactg gtccagcaac tgtagcacct gtacgtgttg cagtttcaac
    14581 gattttttct gccgctgtat caagtgtacg gtgttcgtac gctttcaaac ggatacggat
    14641 ttttttgttt gccatctttt ctcctctcgt ctatttacga taataggcta gctcctcaag
    14701 aaaaccaaca cgagttgcgt ggcaatgcaa cctagcgtgt cgcaacctct tgcatcatag
    14761 ctaaagctgt tcatacacag caccagtata tgatatcaaa aaaaaacagc acacgcaagc
    14821 attattttat tttttttcaa aaaaaatgca accatttctg gttacatttt ttgaatatat
    14881 cgatgatagc gaactttcaa aaacagccac ctaaagatat tatctgctat cgtagccatc
    14941 caaaccccaa gcaaacctaa ttcaaaaact attcctaata aataaccaag aactactctg
    15001 attaaccaca tgcctatagt cgttgtgtaa aatgggagtt tagcatttcc taatccttgc
    15061 caagctgctg tataaaccaa ggtcccaata gtagcaggta ctcctacgaa agatagcaaa
    15121 ataacaatca aacttccttt aatggcagaa ggattatttg taaataactg tgtcaatggt
    15181 ttgccggcaa gaagcataaa acttgatata aggatcatta gaactaagcc aatataatac
    15241 gaactttgaa tagtttcttc tatagattct ctattttttt gtcctacata ttttgcagtt
    15301 aggataattg tagcagttgc gatgccaagt cctggcatat aattaaattg tgtcaaggtt
    15361 tctccaatag catttcccgc tacaacattt gtacctaact gaacaacaat agcaactata
    15421 acaacatctc cagctcgcat catcagacgt tctcctgctg atggtaaggc aagattccaa
    15481 atttgtgcac tgattttcca agtcattctt tttattattt ttttaatagg aagatacttc
    15541 gcaaggatac aaataccaat taatcttgcg ataagagttg atactgccac tccgaccaaa
    15601 ccatagcgcc actcaaaaat agcaaaacct gataagactg cattgaggaa attgacaaaa
    15661 atactgacat acattggtaa acgaggttgc ccttgcacac gtaaaaatga gcctagggtg
    15721 gttaatattc ctaaagtaac aacacctcca cccacaattg ctaaatataa accaccaact
    15781 tgtgcgacac tttttgttgt ccctaaaagt ttaaagaaag tttgtccaaa aacaatcgag
    15841 ataattccta atactgcccc tatcagtgat gttagaaaaa tggcttgaga acatactgaa
    15901 attgcatcat ccttctcact acctgctaac gacttggcca atagacttgc tatactcgcc
    15961 cctaaagcta taaaaatagc ttgataaata gtaattatat tattagcaac tgaaacacct
    16021 gatactgcta caacacctaa ctgagccact agataattat caactactcc catgagcatt
    16081 tgtaagatat tttcaatcat agctggtata gctaactgaa taatctcttt actataacgc
    16141 acagtatctc ctttctatta cccaggatca ttaaaaagag agtgatggga aaatttctaa
    16201 aatgtttctt tctcgctcta aatattttaa aacaatcgta tttttattat tgtttcaaac
    16261 ctagacactc ttcaaccgtt tctcgcatat ttgaggtagg tactaccatt ctatggatta
    16321 cttcggcctt ttgtagatct gtaaccgcct taggtaaaga aacacctgac aaatcgttta
    16381 agattgaaat agcagcgaaa tcctgtatct cttctttatt tgtaatagcc tttgtaacaa
    16441 cacacgggaa cttataagga cttgctgttg acacaattac tgttggagtt tgatctttag
    16501 tttctgtctg ataagcttga taaacagctg atgctacagc tgtatgcgga tcctctatat
    16561 actgatactg gtcaaataaa cgtttgatgt ctaattcaac aaattgttct gtcgcaaatc
    16621 ccgctacaaa gctctctaaa atatttgctt gcctagcctc taaggcatac tccccagtag
    16681 tcactaaatc ttccattaat tttttagtcg tttctgcatc atcgcctaaa aggtgaaaga
    16741 ttaatcgttc tagattagag gagaccaaaa tatccataga gggacttgat gtcactttaa
    16801 attctcgatt tttatcgtag gtttgtgtct taaaaaaatc tgttagaacg ttattatcat
    16861 ttgaggcaca aatgagtttg gtaacaggta atccaatttg actagcataa tatgctgcta
    16921 aaatattacc aaaatttcct gtaggtacac taaaattaat tagttgacca attgagattt
    16981 cctttgactt aactaattga gcatatgcat atatatagta aacaatttga ggaacaaggc
    17041 gaccgatgtt cattgaattg gctgatgata actgcatatg ctgctgtgat aattttgatc
    17101 gaagaaggga attattgaac atttctttaa ccgaagtctg tgcatcatca aaatttcctt
    17161 caatcgcaac aacatgggta ttttgcccgg cttgtgttat catctgcaat tcttgaatat
    17221 aactaacacc atgtttagga taaaatacaa taatctcagt tcctggaaca tctgcaaaac
    17281 ctgccatcgc tgccttacca gtgtcacctg atgttgctgt taaaataacg attttatcat
    17341 caaccccttg ttttttggct gctgttgtta gcaaataagg aagaatggat aaagccatat
    17401 ctttaaatgc aattgtagga ccgtgaaata attcaaggtg ataccggtct ccaatcttta
    17461 ctattggggc tatttcagtt gtatcaaatt tagtatcata agcttgacta atacagtaat
    17521 ccaattcttg ttcagtaaaa tcatcaaaaa aagctgacaa aacgagttta gccacttctt
    17581 ggtaggaagc atccttaagt ttagtgaaat ctaaatcaac ttttggaaat gtgatggggg
    17641 taaagagtcc tccatcgtca gctaatccct ttagaatcgc ttgactagct gtcactttat
    17701 tactagcatc acgtgttgat tgatatacta ttgccataag aatccctctt tatataaata
    17761 tcttttagtt attgtaacac aatttaataa catgtccatt gttcgttata taattaaccc
    17821 ccctttatta aaataaaggg gggcgtaata tcattatcac tttgtaaagt cgagaacttt
    17881 acgtccttga atcaatcctc tttccatttc atcgaatact tgaggagctg tatccacagg
    17941 cactttttca accactggta ctaccaaacc ttctgctcca aatgcgaagg cttcttctaa
    18001 atctttacga gttccaacta aggaaccaac cacacgaatc ccatcaagta ctgtttttac
    18061 aatactcaat tccatatatt ctgatggaag accaacagct accactgtac ctccagcacg
    18121 aacgctgtca atcgcctgat taaacgcaac tttagaaaca gcagtcacaa ccactccatg
    18181 acaaccgcct gtcttttctt gaatataggc cgcaacatct tttatttctt tgccattaac
    18241 tgtcaaatct gctccaacct ctttagctaa ttgaagttta tctgcgttaa tatcaacagc
    18301 tacaacatga gcattgaata ctttttttgc atattggact gctaagtttc caagaccacc
    18361 tgcaccatac actgcaatcc actgaccagg agcagctcca gcttctttga tagccttgta
    18421 tgttgttact ccagcacaag tgattgatga tgcttgagct ggatctaatc cctcaggaac
    18481 cttaaccgca tagtccgcgg taacgatagc gtattcactc ataccaccat caacactgta
    18541 tccagcattt ttaacactac gacaaagcgt ctcacgtcct gtagtacagt attcgcaatg
    18601 accgcaccct tcaaagaacc atgcaataga gacacgatca ccaaccctca aagacgttac
    18661 gccttctcca atttcttcta ctataccaat gccttcgtga cccaaaatgc gtcctggtac
    18721 ctggccgaaa tctccatgtg caacgtgtaa atcagtatgg cacacaccac agtattctac
    18781 ttttacaagt gcttcaccat gaccaacttt tggaagatca tgctcaacaa cttcaacacc
    18841 tgtactagct tgattaacaa caaccgcttt catgataaaa cctcctagaa ataaatatat
    18901 cttatttgta atcgtttaca ttttatattg tattattatt cacaaactat gtcaagtgat
    18961 tttcagaaaa ttttttgaaa atattattaa ctggttaatc tttttgaaac taaaaatccc
    19021 actcaataga gtgagattct tcgactgttg ttacttacgg cgtcctggac gttcctcata
    19081 accataataa gcatcttcga tgatttcttg catgtgatct accattggaa ggcgtggatt
    19141 tgctggtgaa cattgatctt catatgctaa gaaagctagt tcacgagatt tttctttcca
    19201 ttctttttca tcaatgcctt ggtcacggaa attcatctta atacctaaac gtgttccaag
    19261 gtcatacaca gcttttgcat atgattcgac agcttcttct ggtgttgctg ctggtaagcc
    19321 gagtaattta gcgatatctt gatacttttc atcagcacga tagtaattgt acttaggcca
    19381 tgttgctgtt ttagctggac gagttccatt atagcggata acatacggca aaagaattgc
    19441 atttgtacgt ccgtgtacgg tatggaattg tgcgccaatt ttatgcgcca ttgagtgaga
    19501 aatacctaag aatgcattgg cgaaagccat acctgccata gttgacgcat tgtgcatctt
    19561 ttcacgtgct tcaaaatcag catcttttac tgagcgttct aagtagtcaa atacaatttt
    19621 aattgcttga agagcaagtc cgtctgtata atcatttgcc atctgtgaaa cataagcctc
    19681 tgttgcatgt gtcaatacat ccatacccgt atcagccgca ataaaaccag gaacagtcat
    19741 aacaagggcc ggatcaacga tagctacagt tggtgttaat gaataatcag cgattggata
    19801 tttacgatta tttgctttgt cagagataac ggcaaatggt gtcacttcag aacctgttcc
    19861 agaagttgtt ggaatagcaa caaattttgt tttcttacca agttctggga atttgaacgc
    19921 acgtttacgg atatccatga atttttgaac gaggtcatgg aaatcaactt ctggttgttc
    19981 atagaagagc cacataactt tcgcagcatc cataggagaa ccaccaccta atgcaataat
    20041 agtgtctggt ttgaaggtac gcattaaatc agtccctttc attactgttg taatgtctgg
    20101 atctggttct acttcagcaa aaatctgata aacaacttta ttgcgacgaa gatcaagttg
    20161 ttcaatgata cgatccaaga aaccaagctc aaccatggcg tgatctgtaa caatcatgac
    20221 acgttcaaca tcgcgacatt tttgcaaata ttgaatagaa tcacgttcga agtatgtttt
    20281 tgaagggact ttaaaccatt gcatattatt tctacgtctt cctacttttt tgatattcaa
    20341 gagatttata gcactaacgt tatcaccaac tgagttacgt ccatacgaac cacaaccaag
    20401 agtcaatgat ggcaagaaag cattgtaaac atccccaata ccaccaaaag tagaaggaga
    20461 attccaaata acacggatag cacgaattct agttccaaat tcacgagcta agtctgcatc
    20521 ttttgtatga atagcagctg agtgccctag accattaaac tcaaccattt gacgagcttt
    20581 ttcaacacca tcttctgtag attcagcttt caatactgca ataacaggtg atagtttttc
    20641 acgtgtcaaa ggttcttttt cactaacttc agtacattct gcggcaagaa tatttgttcc
    20701 ttctggaaca gtaaatccag cttgttcagc aatccaaact gctgattttc caacaatatt
    20761 tggatttaat tttgcaccag cacagttttt gctatttgct tttgcaccaa aacagaattc
    20821 ttcaagaaga gctttttctt tcttattaac gaaataggta tgataagatt taaattcttc
    20881 aacaaattct ttataaattt ctttatcaat aataacagcc tgttctgacg cacataccat
    20941 accgttatca aatgatttag acataacaat atcgtgagca gcttgacgga tattagctga
    21001 tttttcaaca taagctggaa cattcccagc acctacacca agggcaggtt tcccacatga
    21061 gtaggcagct ttaaccatcg cattaccacc tgtcgcaagg atagtggcaa taccatcatg
    21121 tttcatcaaa gcattagttg catcaatcga tggttgttca atccattgga cacaattttc
    21181 aggagctcca gcagcgatag cagcatcacg aacaatacgt gctgcatgtg ctgatgattc
    21241 ttgtgctgat gggtggaagg caaaaataat tggattacgt gttttaagag agattaatga
    21301 cttgaaaata gcagttgaag tcgggttggt tgttggcgta ataccacaga caactcccac
    21361 tggttcagca attaatgtca agcctgtgac atcgtcttct tcaataactc ccactgtctt
    21421 tgtatgacgc atgttgttaa cgacgtgttc acatgcaaat aaattttttg tcgctttatc
    21481 ttcaaagaca ccacgaccag tttcttctac tgcatgaaga gcaagttctc catgggcatc
    21541 caaggctgca acagaagctt tagctacaat ataatcaact tgttcttgat taagttgacg
    21601 catctcatca agtgctttta aaccgttttg aactaatgta tcaattgcaa gagcaacatc
    21661 tgtagtttct accgctttag ttttttcagt catttgagac tccttttgat ttgttaaatg
    21721 tttcacaaat ttgtttacga gattattata gcatatttaa aaactttgta aatagtttca
    21781 caactttttt gtgaactttt tttcaatctt tcggaaacat ttcttgaaag cgctttaatt
    21841 aaatttgttt tttcattact aattttcaga ttaaagccaa tcgatttcat ttgtatcacc
    21901 ataaacggag gtatctatta ttcgttttca caaaaaagcc gagtacatga ctcggacttg
    21961 tttattttgc agctcgttga gctgattttt gaatagcttt tttgacgtta ctaacatata
    22021 aatcagcccc tgtagtatcg ctatcactat aatgcacacc atctgtattt ccccagattt
    22081 ctggatgttc tgtcgcaacc ttataccagt cagcaaccgt tatgtaatta tattttttca
    22141 ttagcgagag ttcataatca cgcactgtcg taacttgact catattttta gcattataag
    22201 gcgttactat aatcaaacgg tgtcccttag gcaaagattt aataaattgg ctcaagtctg
    22261 tcttgtagtt atctaatgaa ttcacaccaa ctgccaaaac aacaattttg gacaaggctt
    22321 tattttgaat acggttttca aaaatatcaa atgatttact aaaattacga ctcaccgcag
    22381 catctaattg tacttctggt aacaatttat taaaagcagc acttgacctt aaggcaactg
    22441 aatctccgat taccgtcaca tcactaagag cacctgcatc ccctgcggct agtgtatgtg
    22501 ttcggttagt atttgttcta gcttgttgta acgaattctg caataattct gtctcaaaac
    22561 ttcctatact tggagctgtc aacattgtca ctacagtaat aagagttagt acccctccga
    22621 tactaaataa ccactttttg taaggcaaca agtcaaattc ataatcaagg aatttaggtt
    22681 ttctacctaa aataaaaggt tcaatgatat aaaatgataa tgttgagaag actgctgata
    22741 atactactgt caaaataaca gctatccagt ttggggataa acgactaaaa ataatataaa
    22801 atggccaatg aaaaaggtac ataccgtaac tgatatcagc aagataagta atcacgaaag
    22861 gttctgaaat atctggtgta tgctcgtgta aaatacgtgc attgtaaatc attacactag
    22921 caaaaatgct agacaaaaca aaaccaaaaa gatatgttag ccgattatca aaatcaagtg
    22981 caaaagttaa aatcattaga ccagcaaaag cactacccat catgattaaa ttatgcttca
    23041 aagtcagatt tttgatattt ttcttgaatc gtccggtaat ctctcgaata ccagatattg
    23101 tagccaccat cgctcctaaa aagaacggaa atatatgtga taatgtagag aaatatatcg
    23161 ttgaaaaatt atcaacgaaa aaggctctaa caaacatggt aaggaagctg actccaaaaa
    23221 ctcccatgga aacaaggaac aaagtccccc tcaattgctt ttgatctttt gatcttttcg
    23281 ataagagcca taccgttaag ccccataaaa cataaaaatg gacttcaata gaaagactcc
    23341 aagtatgcac aaataagtga gggataaatt gactttcgta gttaccacca gtcaagattt
    23401 cgtaaaagtt gctggtaaaa cctaaagcag tcataatttg agagccaata cttgctctaa
    23461 aatcgctttt aaccagaaat acaaaaggta ttgtcaccaa aaccatcaat actaatggtg
    23521 ggaaaatacg gtaaaaacgt ctacgacaaa aactaacaaa atcaattttt ttcgttttag
    23581 aaaattcatc aatcaacaag gccgtaatca gaaaaccaga aaatgtaaag aagatgtcaa
    23641 caccaacaaa tcctcctgga aaactattct taaagaaatg gtaaagtaaa acaagtagta
    23701 gtcctgttat acgtaccaaa gaaaaccatt ttattctcat ttttgataaa tcccctgttt
    23761 aaatgtcccc ttatagactt tcttattttt agcattgagg cgaccttgtc catcaggttg
    23821 acctttttta aagtcacctg tataagacca gccatgaact gaaacaaatg ttcctttccc
    23881 ttcaaagacc ccatttacaa aatcgccctt atagtaatcc ccattctcat aggttaattt
    23941 acctttacca gtcatatggt gattaacaat agagcccaca tattttatct taccgccatc
    24001 atagctaagg actccttgac tagaagtcgt taaggtaaat actgataaac caaaaacaac
    24061 aataattatc aaagataata tttctagatg agttcgtgtt attttaaaat tcttcatttc
    24121 tacttaatct ctactattat ttattagcgt taaccactgt ttacagccat cactgactag
    24181 acgataggtt tcctcaaaat caccagtata ccatggatca ggaacaccac catcacaaaa
    24241 aaggctaatc ttatcatcat aggttccttg tgatagagct tttaaatcct tgacattttg
    24301 ctcatccata ccgatgatat aatcaaaata tgctaaatcg tctttagaaa taggttgtga
    24361 ccctttatcg ttagtgtaag gaatttgata acgctgcaaa atcgcttgcg ttcccctatg
    24421 tataggattt ccatgctccc atgttgaagt cgcgcgactc tcaaccgaga ggctatcctc
    24481 cttatctaaa ttcgccataa caaactcagc catagggcta cggcagatat ttccaagaca
    24541 aacaaaacag actttcttca tagcaaactc cttttggtct attataacag aaaaaaggta
    24601 cttataatac gtttaactgc tcaaagaata actacaacat atctaatact taacattgca
    24661 tgaaaattaa aaaattaaga ctagataccc tagccttaaa atgacttatg ttttatttat
    24721 caaatcgctg ataacctaaa tgttcgtatg ccttatccgt agctacacga ccggtacggg
    24781 tacgcataat aaaacctttt tgaattaaat aaggttcgta catgtcttca acagtatctc
    24841 gttcttcagt aatattaacg gatagagttc ctaaaccaac aggacctcca ttatacattt
    24901 caatcatggt tcttaagatt ttttgatcga cgtaatcaag cccctcgtga tcaacatcta
    24961 acatcgttaa tgctttatct gtaatattgt catctatcaa accatctccc atgatttgag
    25021 cataatctcg aacacgtttc aataaacggt tagcgatacg tggcgttcca cgactgcgac
    25081 gcgctaattc agaagcagct tcataagtaa ttttcatttc aaaaatatct gctgtacgct
    25141 caataatttc tgtcaaatcg ttttcttcat aatactccat atgccctgta ataccaaagc
    25201 gagcacgtaa gggattagat aacatacctg cacgtgtcgt tgcaccaatt aaggtaaagg
    25261 gcggcaaatc taaatgaaca cttctactag tttctcctgc accgatcata atgtcaatat
    25321 agaagtcttc cattgcacta taaagtacct cttcaaccgc catcggcata cgatgaattt
    25381 catcaataaa aagaacatca cctggttcta aatcatttaa aatggctact aaatcccctg
    25441 atttttcaat tgcgggacct gatgtttgtt tgagattgac acccaactca ttagcaatta
    25501 caaatgccat ggttgttttc cctaaaccag gagggccaaa taataacaca tgatccaatg
    25561 actcatcacg caatttagca gcttcaataa atatttttag ctgatcttta accttatctt
    25621 gtccaatata ctctcttaaa tactgcggac gaagtgtacg ttctaccaat tcttcgtcac
    25681 ccattgcatc actatctaaa aatcttgtca tgatattatt ataacatttg tataggaaaa
    25741 gttaagaact aagttggtgt attagtatct cccttattta aaggaagaat aaactgatct
    25801 tatagttaat ccaacctcaa gaaatcacag aacagcttac ttagcccata ttccaccaac
    25861 ccattcctaa aaacaaatca aatgaaccta aatttgataa tagaacaagt aatactatta
    25921 aaaaacattt tttaatcact ataataaact cctaaataat attttgtacg tttattataa
    25981 atttttagac ctcagagtca caggacatca atgtcctgtt atatcttctt gccactccaa
    26041 ttctaatttt ttaattagat gcttgtcatc aatcattttt gaaaacataa ttgattgttg
    26101 gtaagcattt tctgcagact ttatatcatt gtcaattgta atgaagtatt tccattggta
    26161 cataaaatat aaaggcattt tatgaaaatc atgtgttgac accatcgttt gtcggctaac
    26221 ttctaacatt tctgcaagac actcttcact atttaaacaa agtccaacaa atacacaatt
    26281 caggagtaca tgattaaacc aaaacaaatc ttctgctgta agtttgtggt tctgacttat
    26341 caaagtttta catatagttt gaaatacttc tttagtaaat gctctattgt taaatttaga
    26401 tacaactgca caagttaaaa acaaatctat gataatcaaa tcattaagag tatatttttc
    26461 tttatatttt actttatcaa agcattcgca taaaacctcc acaccaaaat taatgtctcc
    26521 tgtttgatag acttcaaatt tagcttgtaa actgtcaatt gctaagcact cgtcttctgg
    26581 aagattatca tagaattctt cgaaaatatg atcaaactga cattctctta attttaatcg
    26641 ttcagcatct gcataagttg gaattcttaa aatcaaatac ttcaactctt tataccgttt
    26701 aggtaattct aagctctctc ctcctgttaa aatatctaat tttacattca attgtttagc
    26761 tatgtagtga gctttagtca agtttgggat tgactgtcca ctttcaatcc tagctaattg
    26821 tcttacagat aactcagttt catcaccaca aaaatcttcg cgagtaatat ttctagttac
    26881 tctcaaattt cttactttac taccaaaatg ttttaacatt ttcctttaaa aactaactcc
    26941 taaatttttc ttcttattat atattatcat aataaactca ttttaagtat gtttttttaa
    27001 tattaattcc ttttaaatca ataaaaattc cattactggt taaaaaaacg ttaaccagta
    27061 atggaatttt cttatttttc taatcctagt ctttcaaaaa tatcatcaac acgttttgtg
    27121 taataaatcg gattaaatag ttcatcaatt tcttcctgtg taagacaaga ggtaactttg
    27181 gtgtcttctt ctaaaagtgg tttgaaatcc acttgattgt cccaggaata agctgtctta
    27241 ggttgaacta agtcataagc ttcttctcgt gtcattcctt tttcaatcaa tttgagcata
    27301 acacgttgac tataaatcaa accaaaagtt gattccatat tgcgcatcat attttccggg
    27361 aagacagtca agttcttaac gatattgcca aaacggttga gcatgtagtc aatcaagata
    27421 gttgtgtctg gtgtgatgat acgctcagct gatgagtgcg aaatgtcacg ctcatgccaa
    27481 agtgacacgt tctcataagc tgtcaccatg tgaccacgaa tcacgcgcgc tagccctgtc
    27541 atgttctctg aaccgattgg gttgcgtttg tgaggcatag cagagctacc tttctgacct
    27601 ttggcaaaga attcttcaac ttcacgttgt tctgattttt gcagaccacg aatctctgtc
    27661 gccatacgtt cgatagatgt tgcgatgctt gcaagtactg caaaatattc tgcgtggagg
    27721 tcacgtggaa gaacctgtgt tgaaatttct tgcggacgga tgcccaattt gtcacagaca
    27781 tatttttcca caaatggagg aatgttggca aagttaccaa cggcacctga aatcttacca
    27841 gcttccacac ctgcggcagc atgttcaaaa cgctcaatat tacgtttcat ctcgctgtac
    27901 caagtcgcca acttgagacc gaaagtcgtt ggctcggcgt gaacaccgtg ggtacgaccc
    27961 atcatgatgg tgaacttgtg ctccttagcc ttgtcagcta cgatgtttgt gaaattctca
    28021 agatcacgac ggataatgtc gttagcctgc ttgtagaggt aaccgtaggc agtgtccacc
    28081 acgtcagtcg acgtcaaacc gtagtgcacc cacttgcgct cctcaccaag cgtctcagaa
    28141 accgcacgag tgaaagccac cacatcgtga cgcgtgtcct gctcaatctc aagaatgcgg
    28201 tcaatgtcaa aatccgcctt ctcgcgaatc ttagccacat cctctttagg aatctcaccc
    28261 aactcagccc atgcctcgtc agccaaaatc tcgacctcca accaagcacg gtatttattt
    28321 tcctctgtcc aaattgccgc catctcaggg cgtgaataac gtgaaatcat tgtttgtctc
    28381 ctttaactta tttcattaaa atactagaaa tgacattgcc taacacccca gttatggtat
    28441 tctgaacata atcatttttg aagaaactag acattttatt gacaaaattt ttcaattttc
    28501 tttcatcctc tgcctcacta atttccaaca attctgaata tatcgcttta tccgcagtag
    28561 atagctgagt gttaataatt aaatttaact gctcaaccat atcagttcat ggaatttcca
    28621 cacttttatg tatatcggca ttattaatat tagaatcttt tatgatagta gaaccatttg
    28681 atatatcccc agataaacta acttgactac cgttatcaat atttgttcca actatattac
    28741 cactacctga gacatcattt cgactagatc cagattcata attatcgccc tttatatttt
    28801 ggttttttat atcaattttt atattttgat ttttgttatt gaacgatttt gtaattacat
    28861 cgaaaacttt atctctaaaa ttcgaatctt taaaataaag aa
//
LOCUS       DATROQ010000014        27257 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.014, whole genome shotgun sequence.
ACCESSION   DATROQ010000014 DATROQ010000000
VERSION     DATROQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27257
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.014"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(65..1285)
                     /locus_tag="VBC94_000603"
     CDS             complement(65..1285)
                     /locus_tag="VBC94_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEO6978897.1"
                     /translation="MKNLNRYVVAVSGVVLHLMLGSTYAWSVFRNPIISETGWDISSV
                     SFAFSLAIFCLGMSAAFMGHLVERFGPRIMGMISAILYGAGNVLTGLAIETQQLWLLY
                     VAYGILGGIGLGSGYITPVSTIIKWFPDRRGLATGFAIMGFGFASLVTSPLAQSLLIR
                     IGVGKTFYILGLVYFFVMMIASQFIKQPPQEKITILTHDGKKNAMNSQIITGLKANVA
                     IKSKTFYIIWLTLFINISCGLGLISAASPMAQDLAGYSAESAALLVGVLGIFNGFGRL
                     LWASLSDYIGRPLTFIILFIVNFIMTSSLFLSFNAIVFAIAMSILMTCYGAGFSLLPA
                     YLSDIFGTKELATLHGYSLTAWAIAGLFGPLLLSKTYSWGNSYQLTLMVFGFLFLFGL
                     LLSLYLRKLTTKVV"
     gene            complement(1472..2839)
                     /locus_tag="VBC94_000604"
     CDS             complement(1472..2839)
                     /locus_tag="VBC94_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEO6978898.1"
                     /translation="MKLSLHEVAKVVGAKNQVSEFEDVPLGNIEFDSRNISEGDLFLP
                     LKGARDGHEFIEMAFDNGAIATISEKEIEGHPYLLVSDALKAFQVLAQYYIEKMNVDV
                     IAVTGSNGKTTTKDMIAAILSTTYKTYKTQGNYNNEIGLPYTVLHMPEDTEKIVLEMG
                     QDHLGDIHVLSEIAKPRIAVVTLIGEAHLEFFGSREKIAEGKMQITDGMSSDGILIAP
                     GDPIIDPYLPANQMTIRFGHDQELQVTELKEEKHSLTFKTNALEHQLRIPVPGKYNAT
                     NAMVAAYVGKLLAVAEEDIVDALENLQLTRNRTEWKKSANGADILSDVYNANPTAMRL
                     ILETFSAIPNNDGGKKIALLADMKELGEQSVDLHNQMIMSIRPDSIDTLICYGQDIEG
                     LAQLASQMFPIGKVYFFKKNQEVDQFDQLLAKVKDTLKEKDQILLKGSNSMNLSKIVD
                     ILEVG"
     gene            complement(2986..4032)
                     /locus_tag="VBC94_000605"
     CDS             complement(2986..4032)
                     /locus_tag="VBC94_000605"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEO6978899.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIT
                     QVGQFIKTQEFDEMPSSDEKLMTNQTVDLDKMVRPSDIYDDNAIVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKQVLATVGVPQVAYQTYFEGDDLEHAIKL
                     SLETLSFPIFVKPANMGSSVGISKATDESSLRSAIDLALKYDSRILIEQGVTAREIEV
                     GILGNNDVKTTFPGEVVKDVDFYDYDAKYIDNKITMDIPAKVDEATMEAMRQYASKAF
                     KAIGACGLSRCDFFLTKDGQIFLNELNTMPGFTQWSMYPLLWENMGLTYSDLIEKLVM
                     LAKEMFEKRESHLI"
     gene            complement(4173..4769)
                     /gene="recR"
                     /locus_tag="VBC94_000606"
     CDS             complement(4173..4769)
                     /gene="recR"
                     /locus_tag="VBC94_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEO6978900.1"
                     /translation="MLYPTPIAKLIDSFSKLPGIGTKTATRLAFYTIGMSDEDVNEFA
                     KNLLAAKRELTYCSVCGNLTDDDPCLICTDKTRDQSVILVVEDSKDVSAMEKIQEYNG
                     LYHVLHGLISPMNGISPDDINLKSLITRLMDGQVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            complement(4784..6829)
                     /locus_tag="VBC94_000607"
     CDS             complement(4784..6829)
                     /locus_tag="VBC94_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEO6978901.1"
                     /translation="MLNRKKRYRLTVKKQNASIPRRLNLLFFIIVLLFTVLILRLEQM
                     QIGQQSFYMKKLTALTSYTVKESKARGQIFDAKGVVLVENDERPTVAFSRGNNISSQS
                     IKELANKLSHYITLTEVASSDRAKRDYYLADKANYKKVVESLPDSKRYDKFGNHLAES
                     TVYANAVAAVPVSAINYSEDELKVVALFNQMNATPTFGSVKLSTGELSDDQIKKLDAD
                     KKELLGISVTSNWHRRKKGTSLSDILGTISTEKAGLPREEVKKYLKKGYSLNDRVGTS
                     YLEKQYEDDLQGIRQIRKVVVNKKGKVVSDNITQEGKSGRNLKLTIDLNYQNKVESIL
                     KQYYGSELSSGRASFSEGMYAVAIEPSTGKVLAMAGLKNDHGNLVDDSLGTIAKNFTP
                     GSVVKGATLSSGWENKVLRGNEVLYDQEIANIRSWFTRGLTPISAAQALEYSSNTYMV
                     QVALRLMGQDYNTGDALTDRGYQEAMAKLRKTYGEYGLGVSTGLDLPESEGYVPGKYS
                     LGTTLMESFGQYDAYTPMQLGQYISTIANNGNRLAPHVVSDIYEGNDSNKFAQLVRSI
                     TPKTLNKIAISDQELAIIQEGFYNVVNSGSGYATGTSMRGNVTTISGKTGTAETFAKN
                     INGQTVSTYNLNAIAYDTNRKIAVAVMYPHVTTDTTKSHQLVARDMIDQYISQFTGQ"
     gene            complement(6958..7650)
                     /locus_tag="VBC94_000608"
     CDS             complement(6958..7650)
                     /locus_tag="VBC94_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEO6978902.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIQ
                     AAGIEFDLAFTSVLKRAIKTTNLALEAADQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPDMAKDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKQLSDDEIMDVEIPNFPPLVFEFDE
                     KLNLVSEYYLGK"
     gene            complement(7827..8585)
                     /locus_tag="VBC94_000609"
     CDS             complement(7827..8585)
                     /locus_tag="VBC94_000609"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEO6978903.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFIEAVASKLPSSELVEAGIAAP
                     ALTLSTVLEAAKGSELKIAAQNSYFENSGAFTGENSPKVLAEMGTDYVVIGHSERRDY
                     FHETDQDINKKAKAIFANGLTPIICCGESLETYEAGKAVEFVGAQVSAALAGLSEEQV
                     SSLVIAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQAVADKVRVQYGGSVKPE
                     NVAEYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(8766..9962)
                     /gene="tuf"
                     /locus_tag="VBC94_000610"
     CDS             complement(8766..9962)
                     /gene="tuf"
                     /locus_tag="VBC94_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEO6978904.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPTSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDEKYEDIIMELMSTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEVEIVGIKEDIQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVLAKPGSINPHTKFKGEVYILSKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIEVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            complement(10314..11582)
                     /locus_tag="VBC94_000611"
     CDS             complement(10314..11582)
                     /locus_tag="VBC94_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEO6978905.1"
                     /translation="MKIDKRHLLNYSILIPYLILSILGLIVIYSTTSATLIQLGANPF
                     RSVINQGVFWAVSLVAIIFIYKLKLNFLKNSKVLTMAVLVEVFLLLIARFFTQEVNGA
                     HGWIVIGPISFQPAEYLKVIIVWYLAFTFARRQKKIEIYDYQALTKGRWLPRSLSDLK
                     DWRFYSLFMIGLVIAQPDLGNGSIIVLTVIIMYCISGIGYRWFSALLGLIVVGSTLFI
                     GTIAVVGVETMAKVPVFGYVAKRFNAFFDPFKDLTDSGHQLANSYYAMSNGGWFGRGL
                     GNSIEKLGYLPEATTDFVFSIVIEELGVIGAGFILALVFFLILRIMHVGIKAKDPFNS
                     MIALGIGAMLLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAIGFVLNIDAN
                     EKKELIMKEAEEQYKPQEKNEKIINLDAFK"
     gene            complement(11688..14483)
                     /gene="ppc"
                     /locus_tag="VBC94_000612"
     CDS             complement(11688..14483)
                     /gene="ppc"
                     /locus_tag="VBC94_000612"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEO6978906.1"
                     /translation="MSHPKLESSSNKEIITEEVGLLKQLLDEATQKLIGSESFDKIEK
                     IVSLSSTDDYTGLKETISALSNEEMVIVSRYFSILPLLINISEDVDLAYEINYKNNLN
                     QDYLGKLSTTIDVVAGHENAKDILEHVNVVPVLTAHPTQVQRKTVLELTSKIHDLLRK
                     YRDVKAGIVNQEKWYADLRRYIGIIMQTDTIREKKLKVKNEITNVMEYYNRSLIKAVT
                     KLTAEYKALAAKKGIHLENPKPLTMGMWIGGDRDGNPFVTAETLRLSAMVQSEVIINH
                     YIEQLNELYRNMSLSISLTEVSPELVTLANQSQDNSVYRENEPYRKAFNFIQDKLVQT
                     LLNLKVGSYPKEKFVSRQESSGIVGRYIKSHIAQVASDIQTEELPAYATAEEFKQDLL
                     LVKQSLVQHGQDSLVDGELACLIQAVDIFGFYLATIDMRQDSSINEACVAELLKSANI
                     VDDYSSLSEEEKCQLLLKELTEDPRTLSSTHTPKSELLQKELAIFQTARELKDQLGED
                     IIKQHIISHTESVSDMFELAIMLKEVGLIDANQARIQIVPLFETIEDLDNSRDIMTQY
                     LHYGLVKKWITTNNNYQEIMLGYSDSNKDGGYLSSGWTLYKAQNELTKIGEENGIKIT
                     FFHGRGGTVGRGGGPSYEAITSQPFGSIKDRIRLTEQGEIIENKYGNQDAAYYNLEML
                     ISASIDRMVTRMITNLNEIDNFRETMDGIVAESNAVYRNLVFDNPYFYDYFFEASPIK
                     EVSSLNIGSRPAARKTITEISGLRAIPWVFSWSQNRIMFPGWYGVGSAFKQFIEQDEA
                     NLAKLQTMYQKWPFFNSLLSNVDMVLSKSNMNIALQYAQLAGSKEVRDVFNIILNEWQ
                     LTKDMILAIEQHDNLLEENPMLHASLDYRLPYFNVLNYVQIELIKRLRSNQLDEDYEK
                     LIHITINGIATGLRNSG"
     gene            14692..16491
                     /gene="pepF"
                     /locus_tag="VBC94_000613"
     CDS             14692..16491
                     /gene="pepF"
                     /locus_tag="VBC94_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO6978907.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKALEDIKVFKKN
                     YEGKLNCVEDFTSALMEIEHIYIQMSHIDTYAFMPQTTDFSNEEFAQISQAGSDFATK
                     ANVLLSFFNTALANADIKILDSLENNPHFKATIRQAKIQKQHLLSPEVEKALTNLNEV
                     LNTPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVTYENYFQNHENAEIREKSFRSFSKG
                     LRKHQNAAAAAYLAKVKSEKLIADMRGYDSVFDYLLSEQEVDRSMFDRQIDLIMDEFG
                     PVAQRFLKHIADVNGIEKMTFADWKLDIDNELNPEVSINDAYDLVMKSVAPLGKEYSQ
                     EVERYQKERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSFFNTHMSTYYVEAPSTFNELLLSDYLENQFDTARQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGAEQLNAIMKEVLTQFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLNLKNNPNGAKEWLAFLKSGGSCTPLETALLIG
                     ADISTDKPLRDTINFLSNTVDQIINYSNLIKQG"
     gene            complement(16550..16939)
                     /locus_tag="VBC94_000614"
     CDS             complement(16550..16939)
                     /locus_tag="VBC94_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEO6978908.1"
                     /translation="MKKYIKLFLLTVFATTLVACGQPSTSNKTTTSSTLEVGKVELVV
                     KEDTNVLSEKVVYHKGDTVLDVLKANYKVKEKDGFITSIDGISQDETKGLYWMFKVNN
                     KLAPKAANQIKVKKNDKIEFYQEVYKK"
     gene            complement(16923..17447)
                     /locus_tag="VBC94_000615"
     CDS             complement(16923..17447)
                     /locus_tag="VBC94_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6978909.1"
                     /translation="MIKNSTLTKLTRVAILSALCVVLRYAFAPLPNIQPITAIFLITV
                     VLFDLKEGVATVTVTMLVSSFLMGFGPWVFLQIISFTLILCLWKFLIYPLTKAVCFGK
                     ITEVVLQTFFAGGLGVVYGVIIDTCFAWLYHMPWWTYVLAGLSFNMAHALSTCLFYPL
                     LLPILRRFRNEKIH"
     gene            17604..18452
                     /locus_tag="VBC94_000616"
     CDS             17604..18452
                     /locus_tag="VBC94_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEO6978910.1"
                     /translation="MRRRIKPIVVAVFFSLFGLLLIIGHLHSTNTLKKELVEAKKTIP
                     SVKASKVPQKSTSSKDKEFVLKPIIDVSGWQLPKEIDYDTLSKNISGVVIRIFGGSKI
                     SKTNNAAYTTGIDKSFKTHIKEFQKRNIPVAVYSYALGSSVKEMKEEAQIFYKNAAPY
                     KPTFYWIDVEEETMSNMNKGVQAFRKELKRLGAKNVGIYIGTYFMTEQGISVKGFDAV
                     WIPTYGSDSGYYEAAPQTELKYDLHQYTSQGYLPGFNQPLDLNQIAVNKDKKKTYEKL
                     FGKVKD"
     gene            complement(18485..19102)
                     /locus_tag="VBC94_000617"
     CDS             complement(18485..19102)
                     /locus_tag="VBC94_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEO6978911.1"
                     /translation="MEILIAGGSGFLGKQIIKAALTKGHKVAYLSRHEGKGDIFKDPR
                     LTYIKGDITEADKIHLEHRNFDILIDCIGAIKPNQLDELNVKATQKAVALCHKNQIPK
                     LVYISANSGYSAYIKSKRKAEQIIKASGLDYLFVRPGLMYGEERPLSIFQAKCIKLFS
                     HLPFLGIVVQKVFPTKVVTVAEAIVTSLRKKPTQKILSIEELNNK"
     gene            19396..19869
                     /locus_tag="VBC94_000618"
     CDS             19396..19869
                     /locus_tag="VBC94_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEO6978912.1"
                     /translation="MSKKNKIKKTLVDQILDKAKIEHDSLQLDALQGDLPNGIQKQDI
                     FKTLALIGDKTGPIIGILPLTEHLSEKKLAKISGNKKVQMIPQKDLQKITGYIHGANN
                     PIGIRQKHNYPIFIDTIALEKQELIVSAGEIGRSIRINSEVLADFVNAKFADIKE"
     gene            19891..20532
                     /locus_tag="VBC94_000619"
     CDS             19891..20532
                     /locus_tag="VBC94_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO6978913.1"
                     /translation="MKFYLVRHGKTQWNLEGRFQGANGDSPLLEEAIEELEELGQYLS
                     SIHFDAVYSSDLGRARDTVNILNDANSCPKEIHYTSQLREWALGTLEGCKIATMQAIY
                     PRQMTAFYQNPLQFKHDMFGAESLYQTTHRVESFLRSLASKNYDKVLIVGHGANLTAS
                     IRSLLGYQYGSLHYKDKLDNASLTIIETHDFKDFNCLTWNDKSYLRQEVKMTH"
     gene            complement(20568..21470)
                     /locus_tag="VBC94_000620"
     CDS             complement(20568..21470)
                     /locus_tag="VBC94_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6978914.1"
                     /translation="MITSIVFDVDDTIYDQQAPYRIAVEKCFPDFDMSAINQAYIRFR
                     HYSDIGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEATGIYFQEIYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVKKLGLYDYVDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPSTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRSLKPGIKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDINDKSNPVLEMGLNNGLMMAAERLLESNMS
                     VDKVVILLRLTAKQEKVLRMKYAR"
     gene            21645..23135
                     /gene="lysS"
                     /locus_tag="VBC94_000621"
     CDS             21645..23135
                     /gene="lysS"
                     /locus_tag="VBC94_000621"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEO6978915.1"
                     /translation="MSNQHIEELNDQQIVRREKMAALTEQGIDPFGKRFERTATSGQL
                     NEKYADKSKEDLHDIEETATIAGRLMTKRGKGKVGFAHIQDREGQIQIYVRKDSVGEE
                     NYEIFKKADLGDFLGVEGQVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDIETI
                     YRKRHLDLISNRDSFDRFVTRSKIISEIRRFMDSNGFLEVETPVLHNEAGGASARPFI
                     THHNAQDIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEAYQ
                     AYADYQDIMDLTEGIIQHVTKTVKGDGPINYQGTEIKINEPFKRVHMVDAVKEITDID
                     FWKEMTLEEAQALAQEKNVPLEKHFTTVGHIINAFFEEFVEDTLIQPTFVFGHPVEVS
                     PLAKKNDTDPRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQASAKELGDDEATG
                     VDYDYVEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(23210..23680)
                     /locus_tag="VBC94_000622"
     CDS             complement(23210..23680)
                     /locus_tag="VBC94_000622"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEO6978916.1"
                     /translation="MTIIEGQLVANEMKIGIVVSRFNELITSKLLSGAVDGLLRHGVS
                     EEDIDIVWVPGAFEIPYMARKMALYKDYDAIICLGVVIKGSTDHYDYVCNEVTKGIGH
                     LNSQSDIPHIFGVLTTDNIEQAIERAGTKAGNKGYDCALSAIEMVNLDKKLRER"
     gene            complement(23695..24888)
                     /locus_tag="VBC94_000623"
     CDS             complement(23695..24888)
                     /locus_tag="VBC94_000623"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEO6978917.1"
                     /translation="MAFSPIKKLLQDIKSGEMVVLMDDENRENEGDLICAAEMVTKES
                     INFMAKFGKGLICLPLSNYYAEKLELAQMASHNTDNHETAFTISIDHLSTSTGISAED
                     RALTAKMVANDSSKAKDFRRPGHLFPLLAKEGGVLARNGHTEATVDLCRLAGLKECGL
                     CCEIMAEDGSMMRKDELLAFAQKHDLAIATIKQLQDYRRQEEGGVVREIEIQLPTQFG
                     HFKAYGYSEVVANKEHVALVKGDISSGEDVLCRLHSECLTGDVFHSLRCDCGEQLANA
                     LQQIEAEGRGVLLYMRQEGRGIGLINKLKAYHLQEEGLDTLEANLALGFEGDERDYGV
                     SAQLLKDLGINSINLLTNNPDKIQQLEAEGICVKNRVPLQVAATAYDLNYLKTKKEKM
                     GHLLD"
     gene            complement(24906..25556)
                     /locus_tag="VBC94_000624"
     CDS             complement(24906..25556)
                     /locus_tag="VBC94_000624"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEO6978918.1"
                     /translation="MFTGIIEEMGQVSRIRNGIKSQQLSIDVPKLVPLLRKGDSVAVN
                     GVCLTVLDKSEKAFIADVMPESMMRTSLAALRLHSKVNLELALRSDSRLGGHFVLGHV
                     DGVGKIEKIQKDDIAVRFSIDAPPSIMSYIIEKGSVALDGISLTVVSFTEHSFEVSVV
                     PHTMAQTNLSLKKVGDLLNIEVDVLGKYAEKFLAPTNRTNHTSSVMDWSFLSENGY"
     gene            complement(25537..26646)
                     /gene="ribD"
                     /locus_tag="VBC94_000625"
     CDS             complement(25537..26646)
                     /gene="ribD"
                     /locus_tag="VBC94_000625"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEO6978919.1"
                     /translation="MMKKEDYMALALKEAEKGMGFVAPNPLVGAVIVKDDRIISKGYH
                     KRFGDLHAERQAIKNADEDISGSTLYVTLEPCCHVGKQPPCTEALIKSGIKKVVVGSL
                     DPNPLVSGKGIALLRKEGLNVEVGILREECDALNERFIFHMTYKQPFVYLKYAMTLDG
                     KIATKTGDSKWISNEHSRQSVQKLRQKCSAIMVGINTVLADNPRLTCRIPKGEALVRI
                     VCDSQLKIPLDSYLVKSAKTIPTWIATCSDNLAQQQTLKEMGCRLIKVPRKDGKLDLK
                     VLMTILGQEGIDSLLIEGGSSLHFSALEAGIVNRLIVFIAPKIIGGLKAKTAISGEGL
                     DWLNQAFRVKDIELSRMDSDVVIEGKVEHYVYRNY"
     regulatory      complement(26840..26954)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         8790 a         5332 c         4264 g         8871 t
ORIGIN      
        1 acaaaactat ttttcaaaga aaaaactctt tggtaaaacc aaagaatttt aaattagata
       61 tatactacac aactttagtt gttaattttc ttaaatatag agataacaat aatccgaata
      121 agaataaaaa accaaaaacc attaatgtca attgatagga atttccccat gaatatgtct
      181 ttgataacaa taggggccca aacagacctg ctattgccca tgctgttaaa ctataaccat
      241 gtaaagtagc taattccttt gttccaaaaa tatcacttag ataagcaggt aataaggaaa
      301 aacctgcacc ataacaagtc attaaaatag acatcgctat tgcaaataca atagcattga
      361 atgacaaaaa taaactagaa gtcataataa agttcacaat aaataatatt ataaaggtca
      421 acgggcgtcc aatgtagtca gagagacttg cccataacag acgtccaaaa ccgttaaata
      481 tccctagtac ccctactaat aaggctgcag attctgcgga atagcctgct aaatcttgtg
      541 ccattggtga agctgctgat attaaaccta agccacacga aatattaata aacaaggtca
      601 accaaatgat gtaaaaggtt tttgatttta tagcgacgtt tgcttttaat ccagtgataa
      661 tttgtgaatt catagcattc tttttaccat cgtgagtcaa aatagttatt ttttcctgag
      721 gtggttgttt aataaattgt gaggcaatca tcatgacaaa aaaatatact aatcccaaaa
      781 tataaaacgt tttacccaca ccaatcctaa tcagtaagga ttgtgcaagc ggacttgtta
      841 ctaaagaagc aaagccaaat cccataatag cgaatcctgt tgctagtccc ctcctatcag
      901 gaaaccattt aataatagtc gatactggag taatataacc tgaaccaagt ccgattcctc
      961 ctaaaatacc gtatgcaaca tacagtaacc ataactgctg agtttcaatg gctaagcctg
     1021 ttaacacatt ccctgctcca tataaaatag cagaaatcat tcccattatc ctaggaccaa
     1081 aacgctctac taagtgtccc ataaaagctg cagacattcc tagacaaaaa atagccaaac
     1141 taaaagcgaa tgaaactgat gaaatatccc aaccagtctc tgagataatt gggttacgaa
     1201 acacactcca agcataagtt gatcctagca ttaaatgtaa aacgactcca gaaaccgcaa
     1261 ctacataacg atttaaattt ttcaaataat cctcctaaaa acgaacttaa gttttatttt
     1321 taaaaatata gttcgcaata aacagattat caaaaaactg aatatatcac cagttttctg
     1381 cttagcatat ctattttcct caataaaact gaatattatt cgtgattaaa aaacaaaaaa
     1441 gttagggtaa cttgttaccc taacccctct attatcctac ttcaagaata tcaactattt
     1501 tagaaagatt cattgagtta gaacctttta ataatatttg atctttctcc ttaagagtat
     1561 cttttacttt tgcaagcaat tgatcaaact gatccacttc ttgattcttt ttaaagaaat
     1621 acactttacc aattgggaac atctggcttg ctaattgagc taacccctca atatcctggc
     1681 cataacaaat caaagtatca atagagtcag gtctgatact cataatcatt tgattatgta
     1741 aatccacaga ttgttctccc agttctttca tatcagctaa aagagcaatc ttctttccac
     1801 catcattatt gggaatagct gaaaaggttt ctagtatcaa tcgcatagcc gttgggttag
     1861 cattgtaaac atctgataaa atatctgctc cattggctga tttcttccat tcggtgcgat
     1921 tacgagttag ttgaagattt tctagagcat ccactatatc ttcttctgct actgctaata
     1981 attttccaac ataggcagcg accatcgcat tagttgcatt gtacttacca ggtactggta
     2041 tacgtaattg gtgctccaaa gcattagttt taaaagttaa ggaatgtttt tcttctttga
     2101 gttctgtcac ttgcaattct tggtcatggc caaaacgaat agtcatctgg tttgctggaa
     2161 gatatggatc aataattgga tctcccggtg caattaatat gccatctgaa ctcataccat
     2221 ctgtgatttg cattttccct tctgcaatct tttctcgact accaaaaaat tctaagtgtg
     2281 cttcaccaat caaagtaaca acagcaatac gtggttttgc aatttcagat aacacatgga
     2341 tatctccaag atgatcctgt cccatctcca aaacaatttt ttcagtatct tctggcatgt
     2401 gtagcacagt atatggtaag ccaatctcgt tattgtagtt tccttgtgtt ttataagtct
     2461 tatatgtggt tgataagatt gcagctatca tatctttagt tgttgtctta ccatttgagc
     2521 cagttactgc aataacatca acgttcatct tttcaatata atactgtgcc aagacttgaa
     2581 atgctttaag tgcatcagac actaaaagat aaggatgacc ctctatttcc ttctctgaaa
     2641 tggttgctat tgctccatta tcaaaagcca tctctataaa ctcatgtcca tctcttgctc
     2701 cttttaaggg aagaaataag tcaccttcac tgatattacg actatcaaat tcaatatttc
     2761 ccaggggaac gtcttcaaac tctgatactt gatttttagc gccaactact ttagcaactt
     2821 catgcaaact aagcttcata tcatttcctt ttctattatt tagcaacaat gttgtagata
     2881 gtatgctatc ccttataaac gaatatactc ctaaattata acacactttc ataacatatc
     2941 atagatttga aaaagctcaa ctaagaaaga cttagttgaa cttttttaaa ttaaatgact
     3001 ctcacgtttt tcaaacattt ctttagctaa cataactaac ttttcaatca aatcactata
     3061 agttagcccc atattttccc aaagcagagg atacattgac cactgagtaa aaccgggcat
     3121 tgtattcagt tcgtttaaga agatttgtcc atctttcgtc aaaaagaaat cacagcgtga
     3181 taaaccacaa gccccgattg ctttaaaagc tttacttgca tattgacgca ttgcttccat
     3241 agttgcttca tcaactttag ctggaatatc catagtaatt ttattatcaa tatatttggc
     3301 gtcatagtca tagaaatcga cgtctttaac aacttcgcca ggaaaagttg tcttaacatc
     3361 attattgcct aaaataccta cttcaatttc acgagctgtc acgccttgtt caatcaaaat
     3421 acggctatca tacttgagag ctaagtcaat tgcagagcga agtgaggatt catctgtcgc
     3481 ttttgaaata cctactgatg accccatatt agccggtttt acaaaaattg ggaaacttaa
     3541 agtttctaaa gagagtttaa tcgcatgttc caaatcatca ccctcaaaat aagtttgata
     3601 tgcaacctga ggtacaccta ctgttgcaag gacttgtttt gttgtaattt tatccatagc
     3661 cacgcttgaa gatagaatat tagtcccaac ataaggcatc cttaaaactt ctaaaaatcc
     3721 ttggatagaa ccatcttccc ccattggtcc atgtaaaacg gggaaaacaa ttgcattatc
     3781 atcatagata tcacttggac gaaccatttt gtctaaatca acagtttggt ttgtcattaa
     3841 cttttcatct gaagatggca tttcatcaaa ttcttgtgtt ttaataaatt gacctacttg
     3901 cgtgataaaa taagttttaa caaaaaattt atcataatta atagcacgca tgacgctttc
     3961 tgcagacagt acagatactt cacgttccgc agagcgacca ccgtaaagca agatcaatgt
     4021 ttcttttgac atatttcttc tcttttctat ctaatctaag attgaccttt taataatacc
     4081 atattttaac ttaactttca aaaatcaatc cacttaaagt tagtattgta aaagaaaaag
     4141 ttaggagatt cccaaccttt tctttataac aattataact ctgtacgatt ttcgattgct
     4201 cgaagcaagg ttacttcatc tgcatattcg atatctgaac caacagctaa acctctcgct
     4261 aatcgtgtca ctttgatacc agctggtttt aagactcgtg aaatatacat tgatgtagct
     4321 tcaccatcag cagtcgcatt ggtagcaaca ataacttctg tcacctgacc atccatcaat
     4381 cgagtaatta aacttttcaa attaatgtca tcagggctaa taccattcat aggtgaaatt
     4441 aagccatgta agacatgata caaaccatta tactcttgaa ttttttccat agctgacaca
     4501 tctttactat cttcaacaac caagataaca gactggtcac gcgtcttatc tgtacaaatt
     4561 aagcaaggat catcatcagt caaattacca caaacagaac aataggttaa ttcccgctta
     4621 gcagctaata aattcttagc aaattcatta acatcttcat cactcatacc aatggtgtaa
     4681 aaagctaaac gtgttgctgt tttagtacct atacccggta attttgaaaa gctgtcaatc
     4741 aatttagcaa ttggtgttgg gtaaagcatt caaatgtcct ctcttattgt cctgtgaact
     4801 gtgaaatata ttgatcaatc atatctcgtg caactaattg atgggatttt gttgtatcag
     4861 ttgtaacatg cggatacatt accgctactg ctattttacg attagtatcg taggcaatag
     4921 cgtttaagtt gtaggtagaa actgtttgtc catttatatt tttagcaaat gtttcagcgg
     4981 taccagtttt accactaatg gttgtcacat tccccctcat tgacgtacca gttgcatagc
     5041 cacttccact attgacaacg ttataaaaac cttcttgaat aatggctaac tcttgatctg
     5101 agatagctat cttatttagt gttttaggag tgattgaacg aaccaattga gcgaacttat
     5161 tagaatcatt cccttcatag atatctgaaa ccacgtgagg tgctaaacga ttcccattat
     5221 tcgcaatagt tgagatatac tgaccaagtt gcattggtgt ataggcatca tactgaccga
     5281 acgattccat tagagttgtt cctaagctgt attttccagg tacataacct tctgattcag
     5341 gtaaatctaa tcctgtagaa acccctaaac catattcgcc gtaagttttc cttagtttag
     5401 ccattgcttc ttgatagcct cgatcagtta aagcatctcc tgtattatag tcttgtccca
     5461 ttaaacgaag tgctacttga accatataag tattagatga atactctagc gcttgtgccg
     5521 cagatattgg agtcaaacct cgtgtaaacc atgaccgtat attagcaatt tcttggtcat
     5581 aaagcacttc atttcctcta agaactttat tttcccatcc tgatgatagc gtcgcacctt
     5641 tcacaacaga tcctggcgtg aaattcttag caatagtgcc taggctgtca tcaactaaat
     5701 tcccatgatc atttttcagc ccagccattg ctaacacttt accagttgaa ggttctatag
     5761 caactgcata catcccttcg gaaaaactag cgcgcccact agataattca ctgccatagt
     5821 attgtttcaa gatactttct actttgtttt ggtaattaag atcaatcgtt aatttaagat
     5881 ttcttcctga cttaccttct tgcgtaatat tatctgaaac aactttacct tttttattaa
     5941 caactacttt gcgaatttgt ctgattcctt gtaagtcatc ttcatattgt ttttcaaggt
     6001 aagaagttcc cactcggtca ttcagtgaat agcctttttt caaatatttt ttaacttctt
     6061 ctcttggtaa acctgccttt tctgtagaaa tagtacctaa tatatccgat aaagaagttc
     6121 cttttttacg acgatgccaa ttacttgtta cagaaattcc taacagttcc ttcttatcag
     6181 catccagttt cttgatttga tcatcactta attcacctgt acttaatttc actgaaccaa
     6241 acgttggtgt cgcattcatt tggttaaata aggcaaccac ttttaactca tcttcagaat
     6301 agttaatagc gctcactgga actgcagcaa ctgcatttgc atatacggtt gattcggcta
     6361 gatggttacc aaatttatcg taacgtttag aatctggtaa actttcaaca acttttttgt
     6421 agttggcttt atctgctaaa taataatctc tctttgctct atcgcttgaa gcaacttcgg
     6481 ttaaggtaat gtaatgtgat aatttattcg ctaactcttt gatagactga gatgaaatat
     6541 tgtttcctct agaaaacgcc acagtaggac gttcatcatt ttcaactaag acaactccct
     6601 tagcatcaaa aatctgacct ctcgcttttg attctttcac tgtataactc gtaagagctg
     6661 tcagtttttt catgtaaaat gattgttgcc caatctgcat ttgctctaaa cgaagaatta
     6721 gaacagtaaa taataaaaca ataataaaaa atagaagatt taatcgtcta ggtatgctag
     6781 cattttgctt ttttacagtg agacgatacc tttttttacg attcaacatt aactccttgc
     6841 tataaaaact ctgactacta ttgtatcata aaaatggttt tttacagaca ctcaaaatgt
     6901 tataaataat aaaaaattca agccaacttg gcctgaattt tttatataaa gattagatta
     6961 tttacctaag taatattctg aaacaaggtt taatttttca tcaaattcga aaacaagtgg
     7021 tgggaagtta ggaatttcaa cgtccatgat ttcatcatct gacaattgtt tgatatgttt
     7081 tacaagagca cggattgagt taccgtgtgc accaacaaac acatttttac catctttaag
     7141 agcaggagca attttatctt cccagaaagg aagagcacgc tctaaagtaa cttttaggtt
     7201 ttctgcatct ggaataacag aatcatctag tgaagcatag cgacgatcag tatgtgctga
     7261 atgttcatca tctttagcca tatctggagg caatacatca tatgaacgac gccaaatatg
     7321 aacttgctca tcaccaaatt gttcagctgc ttctgcttta ttttttcctg tcaatccacc
     7381 gtaatgacgt tcgttcaagc gccatgattt ttcaactggt acccaaagtt gatcagctgc
     7441 ttcaagggca aggttagttg ttttgatggc acgtttaaga actgatgtaa aagcaaggtc
     7501 gaactcaata cctgctgctt gaattaattt cccagcatca atagcttgtt gtgtaccttt
     7561 ttctgaaaga tctacgtcag cccatccagt gaaaaggtta gctttattcc actcagattc
     7621 accgtggcgt gcgaatacta attttaccat tagaaaattt ctcctttaat tttggctata
     7681 ccaatattta ctttaactat tctacaaaaa atagccataa aaatctagtc tgattactag
     7741 ttcaataatc aaaaatagct tattattaac tatctataag aaaaggcata tcacctagag
     7801 tgctatgcct ttagatatca tgtgatttat ttaacaaaat ctaataaggc aaggaagctt
     7861 tctgcttcaa gtgaagctcc acctacaagg gcaccgtcaa cgtctgggca agccatatat
     7921 tctgcaacgt tttcaggttt aactgaacca ccgtattgaa cgcgaacttt gtcagcaact
     7981 gcttgaccga agtctgctgc aacaacgtca cgaactgctt tacacatgtt ttgtgcgtcg
     8041 tcttgagttg ctgatttacc agtaccaata gcccagattg gttcgtaagc gataacaagt
     8101 gatgatactt gctcttctga caaacctgca agagctgctg aaacttgagc acctacgaat
     8161 tcaaccgctt taccagcttc gtaagtttca agagactcac cgcaacaaat gattggagtc
     8221 aaaccattag caaagatagc ttttgctttt ttgttgatat cttggtcagt ttcatggaaa
     8281 taatcacgac gttctgagtg accgataaca acgtagtctg tacccatttc agccaatact
     8341 ttagggctgt tttcaccagt gaatgcacct gaattctcaa aatagctatt ttgagcagcg
     8401 attttcaatt ctgaaccttt agctgcttca agaactgttg ataaagtaag tgctggagca
     8461 gcaatccctg cttcaacaag ttctgatgat ggtaatttag aagctactgc ttcgatgaat
     8521 gcttttgctt cttctggatt tttattcatt ttccagttac cagcgataaa tggtttacgt
     8581 gacattacac atacctcttc tttttttatt tactaatcta tcataccata aattatagtt
     8641 aattaaaagt ttcacgagtc tagtaaaacg ctttaattat agatactaaa aaagagtccc
     8701 tgaggactct tcacaatttg agctgtctga ctctaactgc cgtttgggaa cttaattaag
     8761 ttaaattaag cttcgatttc tgaaacgata cctgaaccaa cagtacgtcc accttcacgg
     8821 attgagaaag tagtaccttg ttctacggcg attgggtgaa tcaattcaac ttcgatagta
     8881 acgttatcac caggcataac catttctgtt cctgctggaa gttcgattga acctgttacg
     8941 tcagttgtac ggaagtagaa ttgtggacgg tagttgttga agaatggagt atgacgtcca
     9001 ccttcttctt tagaaaggat gtaaacttca cctttaaatt tagtgtgtgg gttgattgaa
     9061 cctggtttag caagaacttg accacgttcg atttcatcac gttgaacacc acgaagaaga
     9121 acaccaacgt tgtcccctgc aagaccttcg tcaagttgtt tacggaacat ttcaacacca
     9181 gtaacaactg ctttttggat atcttcttta ataccaacga tttcaacttc gtcgttgaca
     9241 cgaacagtac cacggtcgat acgtcctgaa gcaactgtac cacgtccagt gattgagaat
     9301 acatcttcga ctggaagaag taaaggtttg tcagtatcac gttctggttc tggaatgtac
     9361 tcatcaacag tgctcatcaa ttccatgatg atgtcttcgt atttttcatc gccttcaagt
     9421 gctttaagag ctgaaccttg gataactgga aggtcatcac ctgggaagtc gtattctgaa
     9481 agaaggtcac gaatttccat ttcaaccaat tcaagcaatt cttcatcatc aacaaggtca
     9541 actttgttca tgaatacgat aaggtgttta acaccaactt gacgtgaaag aaggatgtgc
     9601 tcacgagttt gtggcattgg tccatcagtt gaagcaacta caaggatagc tccgtccatt
     9661 tgagcagcac cagtaatcat gtttttaacg tagtccgcgt gtcctggagc gtcgatgtgg
     9721 gcatagtgac gtttttcagt ttcgtactca acgtgtgcag tgttgattgt gattccgcgt
     9781 tcgcgctctt ctggagcagc atcgatagaa gcgtaatctt ttggttggtt aactgaagta
     9841 ggcaagcgac gtgccaatac agttgtgata gctgctgtta aagtagtttt accgtggtca
     9901 acgtgtccga ttgtaccaat gttaacgtgt ggtttactac gatcgtattt ttcttttgcc
     9961 attttaggaa aagcctccaa taaaatatat tttatagata gacggtaggc aatacagtct
    10021 aactttactt atctatttta acaaaatcct ctaaaaaagc aagtatttta tgcttttttg
    10081 cttatttctt tatttcctta aaattcgggt aagattgatt gaggcgtttg ttatctctta
    10141 tatgtatttt ttagtagtta tcatataatc atattgttaa atacatccta atttatagta
    10201 ttagaatatt aatagacact tcttttcttc aaatatcttc tctctattaa ttagagttta
    10261 aatttcaaaa aatacaaaaa tcgagataaa aatctcgttt tttgtttgga tgattattta
    10321 aaggcatcaa ggttgattat tttttcattt ttttcttgtg gcttatattg ctcttctgct
    10381 tctttcatta tgagttcctt tttctcgtta gcatcaatat taagcacgaa tccgatagct
    10441 acagaaagta ccaagaggct gttccctcct tgggataaga aaggaaatgt cactccagtt
    10501 gaggggatta acccagaaat accaccaatg ttaacaaaaa cttgcatcaa tagcattgct
    10561 ccaataccaa gagcgatcat tgagttgaaa ggatctttag cctttatacc tacgtgcata
    10621 atacgtaaaa ttaagaagaa aaccaaagct aaaataaacc ctgcaccaat aactcctaat
    10681 tcctcaataa caattgaaaa gacaaagtct gttgttgctt ctggtaagta ccctaatttt
    10741 tcaatagaat tcccaagtcc tcgaccaaac cagccaccgt tgctcattgc ataatatgaa
    10801 ttagccaact ggtgtcctga atctgttaaa tctttaaatg ggtcaaaaaa agcattaaat
    10861 cgcttagcta cataaccaaa gacagggact ttagccattg tctcaactcc aacaactgca
    10921 atagttccga taaataaagt tgaccccacg acgattagac caagtaaagc tgaaaaccat
    10981 cgataaccaa taccactaat acagtacata ataatgaccg ttagaacaat tatcgaacca
    11041 tttcctaaat caggttgtgc aattaccaaa ccaatcataa ataaagaata aaatcgccaa
    11101 tcttttaggt cgcttagtga tctcggaagc catctccctt ttgtcaaagc ttgataatca
    11161 tatatttcta tctttttttg ccttcgagca aaagtaaacg ctaaatacca tacaatgata
    11221 acttttaaat attcagcagg ttgaaaacta ataggaccaa taacaatcca accatgtgct
    11281 ccattaactt cttgtgtgaa aaagcgcgct attagtagca aaaatacttc taccaatact
    11341 gccatagtca acactttaga atttttcaaa aaatttagtt tcagtttata gataaagata
    11401 attgcaacca aactgactgc ccaaaagaca ccctggttga ttactgaacg aaaagggtta
    11461 gctccaagct gaattagagt tgcactagtt gtcgaataaa ttacaattaa gcctagaata
    11521 gacaatatca aatagggaat gaggatggaa taattcagaa gatgcctttt atcaattttc
    11581 atattgtatt atttccttta atatatcaaa aaattaccat tatgcattat atcatctttc
    11641 gaagtaaagt aaaagggaga atagcaatga tcctccctct atcaatatta acctgaattt
    11701 cgtaaacctg ttgcaatacc attaatagta atatgaatca acttctcata gtcttcatct
    11761 agttggttac tacgaagacg tttaatcaat tcaatttgaa cataattcaa aacattgaaa
    11821 tatggcaaac gataatctaa acttgcatgt aacattgggt tttcttctaa taaattatca
    11881 tgctgttcaa tagctaaaat catatcttta gttaactgcc attcattaag gatgatatta
    11941 aaaacatctc gaacttcttt acttccagct aactgagcat actgaagcgc aatattcata
    12001 tttgattttg ataaaaccat atctacatta gataaaagag aattaaagaa tggccatttt
    12061 tgatacatgg tttgtaattt tgctaagttt gcctcatctt gttcaataaa ttgcttgaag
    12121 gctgacccaa caccatacca acccggaaac ataatacgat tttgtgacca tgaaaatacc
    12181 catggaattg cacgtaaacc tgaaatttct gtaattgttt tacgtgctgc tggccgagaa
    12241 ccaatattta aacttgatac ctctttaatt ggacttgctt caaagaaata gtcatagaaa
    12301 taaggattat caaaaactaa atttctataa acggcattac tttcagccac aataccatcc
    12361 attgtctcac ggaaattatc aatttcatta aggttagtaa tcatacgtgt taccatgcgg
    12421 tctatggaag ctgaaataag catttcaagg ttataatagg ctgcatcttg attaccatat
    12481 ttattctcaa tgatttcacc ttgctctgtt aggcgaattc gatctttgat tgaaccaaaa
    12541 ggttgtgatg tgatagcttc ataagatgga ccaccaccac gaccaacagt tccaccacga
    12601 ccgtggaaaa atgtgatttt aataccgttt tcttcaccta tttttgttag ctcattttga
    12661 gctttatata gagtccaacc agatgataaa tagccaccat ctttattact atctgaataa
    12721 cctagcatta tttcttgata attgttgtta gttgtaatcc atttttttac taatccataa
    12781 tgtagatact gggtcataat atcacgtgaa ttatccaaat cttcgatggt ttcaaataac
    12841 ggtacaatct gaatgcgagc ttgattggca tcaattagtc caacttcttt taacatgata
    12901 gctaactcaa acatatccga aacgctctct gtgtgagaaa tgatatgctg ttttataatg
    12961 tcttcaccca attgatcttt taactcacgt gctgtttgga aaatagccaa ttctttttga
    13021 agtaactcgg atttaggagt atgcgtagaa gataatgttc taggatcttc tgttaattct
    13081 tttaagagaa gttgacattt ttcttcttct gaaagagaac tgtaatcatc tacaatattg
    13141 gcagacttca ataattctgc tacacaagct tcattaatac tagaatcttg acgcatatca
    13201 atagtagcta aataaaatcc aaagatatct actgcttgaa taagacaagc taattcgcca
    13261 tcgacaagag aatcttgtcc atgttgaact aaagattgct tcacaagtaa caaatcttgt
    13321 ttaaactcct cagcagtagc gtatgctggt agttcttctg tctgtatgtc agaagctact
    13381 tgagcgatat gtgattttat ataccttcct acaataccag aagattcttg gcgactaaca
    13441 aatttctctt ttgggtaaga accaactttt aaatttagta gggtttgtac tagtttatct
    13501 tggataaaat tgaaagcttt acgataaggt tcattttccc tatatactga attatcttgt
    13561 gactggtttg ccaacgtaac aagttcggga gagacttcag ttagacttat tgaaagtgac
    13621 atattacgat aaagttcatt tagctgctca atataatggt taataatcac ttcactttgt
    13681 accatagctg ataatcttag tgtctcagca gttacaaagg gattgccatc tctatcacca
    13741 ccaatccaca tccccatagt cagaggctta ggattctcta agtgaatacc ttttttagct
    13801 gccagtgcct tatattctgc tgttaatttg gtaactgctt taataagaga acggttgtaa
    13861 tattccatta cattggttat ctcatttttg actttcaatt ttttctcgcg aatagtgtct
    13921 gtttgcatga taatgccgat gtaacgacgc aagtcagcat accatttttc ttgattcacg
    13981 attcctgctt tgacatctct gtatttacgt aataaatcat gaatcttaga cgttaattcg
    14041 agcaccgttt tacgctgcac ctgagtagga tgagctgtta gcacaggcac aacattgacg
    14101 tgttcaagaa tatctttagc attttcatgg ccagctacaa catcaatagt cgttgataac
    14161 tttcctaggt aatcttggtt aagattattt ttataattaa tttcatatgc taaatcaacg
    14221 tcttctgaaa tattaattaa gagcggcaaa atagagaagt aacgagaaac aataaccatc
    14281 tcctcgttgc ttaaagccga aatagtttcc ttcaaaccag tataatcgtc agttgatgat
    14341 aatgaaacta ttttttcaat cttgtcaaat gattcactac caatcaattt ttgcgttgct
    14401 tcatccaaaa gctgcttcag taaaccgacc tcttcggtaa tgatttcttt attgctacta
    14461 ctttctaatt tcgggtggga caaaccatca cttcctctct taaatctcac tactatgata
    14521 ccattttaaa gtaaaaagta aaccattcta ggatttttag atagaatttt cagattttta
    14581 cttatttatg acagtaatca gtttttcttg ttcgactttt ttgatatact gaggataatt
    14641 gagattaact gattaattgt taattaacaa ctattattaa aggagtatcg catggaactt
    14701 aaaaaacgct ctgaattccc tgaaaatgag ctttgggatc tgactgctct ctacaaagat
    14761 cgtcaagatt ttttactggc tattgaaaaa gctcttgagg atatcaaggt tttcaaaaaa
    14821 aattacgagg gaaaacttaa ttgtgttgag gattttacta gtgcccttat ggaaatcgag
    14881 catatttata tccagatgag tcacattgat acctatgctt ttatgcctca aactactgac
    14941 tttagcaacg aagagtttgc acaaatttct caagcaggat cagacttcgc aactaaagct
    15001 aatgtactac tttctttctt taatactgcc ctagcaaatg cagatataaa gattttagat
    15061 tctttagaaa ataaccctca tttcaaagca actattcgcc aagctaaaat tcaaaagcaa
    15121 cacttattaa gtccagaagt cgaaaaagct ttgacaaact taaatgaagt cctaaataca
    15181 ccatatgata tttatactaa aatgcgtgct ggcgattttg atatggaaga ttttgaagtt
    15241 gacggaaaaa cctacaaaaa tagttttgta acttatgaaa attactttca aaatcacgaa
    15301 aatgcagaga tacgtgaaaa atccttccgt tcattctcga aaggtctacg taagcaccaa
    15361 aatgctgcag ctgcggctta tcttgcaaaa gtcaaatctg aaaagttaat tgctgatatg
    15421 cgtggttacg atagtgtttt tgattatctt ctttccgaac aagaagttga ccgttcaatg
    15481 tttgacagac aaattgatct tatcatggat gaatttggtc cagtagctca acgattcctc
    15541 aaacatatcg ccgatgttaa tggtattgaa aagatgactt ttgcagattg gaaattagac
    15601 attgataacg aacttaatcc cgaagtaagt atcaatgatg cttatgactt agtaatgaaa
    15661 tcagttgctc ctttaggaaa agaatattcc caagaggttg agcgctatca aaaagagcgt
    15721 tgggttgatt ttgcagctaa cgctaataaa gattctggag gatatgctgc ggatccttac
    15781 aaagttcatc cctatgtact catgagctgg acggggcgta tgagcgatgt ctacacactt
    15841 attcatgaga ttggacattc tggtcaattt atcttctcag ataatcacca aagttttttc
    15901 aatactcaca tgtccactta ctatgttgaa gctccatcta cttttaacga acttttactt
    15961 agtgactact tagaaaacca atttgataca gcacgtcaaa aacgctttgc tcttgcacat
    16021 cgcttaactg atacttactt ccacaatttc attactcact tacttgaagc tgctttccaa
    16081 cgtaaagttt acactctaat tgaggaggga gaaacttttg gagcagaaca attaaatgct
    16141 attatgaagg aagtattaac acaattctgg ggagatgcta ttgaaataga tgacgatgct
    16201 gctttgacat ggatgagaca agctcattac tacatggggc tctatagcta tacttactct
    16261 gctggtttag taatctcgac agccggctat ctcaatctca aaaataatcc aaacggagca
    16321 aaagagtggt tagcattctt gaaatctggt gggagctgta cccctttaga aacagcccta
    16381 ttaatcggtg ctgatatttc tactgataag cctctacgcg atactattaa tttcttatct
    16441 aacactgtag atcaaattat taattatagt aatcttatta aacaaggcta aactacagtc
    16501 tttcaaaaac ataaaaacaa gttggagata tctccaactt gtttttatct tattttttgt
    16561 aaacttcctg ataaaattca attttgtcgt tctttttaac tttaatttgg tttgctgcct
    16621 taggagctaa tttattattg accttgaaca tccaataaag ccctttggtt tcatcttggc
    16681 tgatgccatc aattgaagta atgaaaccat ctttttcttt aactttatag ttagctttaa
    16741 gcacatccaa aacagtgtca cctttatggt aaacaacctt ttcacttaac acgttcgtat
    16801 cttctttaac aaccaattca accttaccaa cttctaacgt tgagctagtt gttgtcttat
    16861 tcgatgtaga tggttgcccg caagctacta aagtcgtagc gaatactgtt agcaagaata
    16921 atttaatgta ttttttcatt cctaaatctc cttaaaatcg gtaacaaaag aggataaaat
    16981 aaacaagttg ataaagcgtg tgccatatta aaactaagtc ctgctaatac ataagtccac
    17041 caaggcatat gatataacca agcaaaacag gtatcaataa tgacgccata aacaacccct
    17101 aaccctcctg caaagaaagt ttgaagtact acctccgtta ttttaccaaa acatacagct
    17161 tttgtcagcg gatatatcag aaatttccac aagcataaaa ttaaagtgaa ggaaatgatt
    17221 tgcaaaaaga cccatggccc aaagcccatt aaaaaagaag aaactagcat tgttacagtt
    17281 acagtagcta caccttcttt taaatcgaat aagacaactg taatcaaaaa aatggcagta
    17341 attggctgaa tgttaggcag aggcgcaaaa gcatatctca acacaacaca caaagcagat
    17401 aatatagcta ctctagtcaa tttagttaaa gttgaattct ttatcatgtc tttaattata
    17461 cctcaaaact atgactaccg cttaaaagtc ttttttatta caatttcatt tctaatgtta
    17521 cataaacgac ctcttttttt aaaagaacgt aaataagcct gtaaactttg attttttaaa
    17581 ccttttaagt tacaatatca ataatgagaa gaagaattaa acctattgtt gttgcggttt
    17641 ttttcagttt atttggactt ttattaatta ttggtcattt acattcgaca aatactttga
    17701 aaaaagaatt agttgaagct aaaaagacaa ttccatccgt aaaagcttca aaagtaccgc
    17761 aaaaatcaac atcatcgaaa gataaagagt ttgttcttaa accgattatc gatgtctctg
    17821 gttggcaact tcctaaggag attgattacg atacgctttc aaaaaatatt tcaggtgttg
    17881 ttattcgtat ctttggtgga tcaaagatat ctaagactaa taacgctgct tatacaactg
    17941 gaatcgataa atcgtttaag acccatatca aagaatttca aaagcgaaat atcccagtag
    18001 ctgtctacag ttatgcactt ggttcaagtg ttaaagaaat gaaagaagag gctcagatat
    18061 tttataagaa tgcagctcct tacaaaccaa ctttttattg gattgacgta gaagaggaga
    18121 caatgtctaa catgaataaa ggtgtccaag cattccgaaa agagttaaaa agacttggtg
    18181 ctaaaaatgt tggtatctac atcggtactt actttatgac tgagcaaggt atctctgtaa
    18241 aaggatttga cgctgtttgg attccaactt atggtagcga ttctggatac tatgaagcag
    18301 ctccacaaac tgaacttaaa tacgatttac accaatacac ctctcaaggt tatctaccag
    18361 gattcaatca accgcttgat ttaaatcaaa ttgcagttaa taaagacaag aagaaaactt
    18421 atgagaaact ttttggaaaa gtaaaagatt aaaagctact gttaacagta gcttttttga
    18481 taaactattt attatttaat tcttcaatag aaaggatttt ttgagttggt tttttcctaa
    18541 gcgaagtaac gattgcttct gccactgtca caaccttagt tggaaagact ttttgtacaa
    18601 caatacctaa gaaaggcaaa tgactaaata attttataca cttggcttgg aaaatcgaga
    18661 gaggtcgctc ttcaccatac atcaaacctg gtcttacaaa aagataatcc agaccgcttg
    18721 ctttgattat ctgctctgcc ttccttttac ttttaatgta agctgaatag ccgctattgg
    18781 ctgaaatgta aactaacttt ggtatttgat ttttgtgaca gagtgctact gctttttggg
    18841 ttgctttaac gttaagctca tctagttgat tgggcttaat cgctccaata cagtcaatta
    18901 atatatcaaa atttctatgt tctaaatgaa tcttatcagc ttctgtaata tctcccttaa
    18961 tgtaggttaa tctaggatcc ttaaatatat cacctttacc ttcatgcctt gataagtaag
    19021 ccaccttatg cccttttgta agcgctgctt ttattatctg cttccctaga aaaccactac
    19081 cacctgcaat cagtatttcc atagttccct ctccttcatt ttgtaaattt agtatagaaa
    19141 tttttatatg ccttgtcaaa gtaatcgttt aatacctgac cagaatatga tttcaagtca
    19201 ttacattagg acacttcata tttagctatt tataaaactt ctaatttaac attccaaata
    19261 tactgttgcg atatttatta tcattattcc ctgaaatggt attcaagctt atttctaaga
    19321 ctcagtagaa atttactata atcatcttta aatgttataa taaatatgaa acaaacttat
    19381 tagttaaggg tcattatgag taaaaagaat aaaatcaaaa aaactttagt tgatcaaatt
    19441 ttagataaag caaaaatcga acatgatagt ctacaattgg atgctttaca gggagattta
    19501 cccaacggta ttcaaaaaca agatatcttt aagacattag cgctaatcgg tgataaaact
    19561 ggtcccataa taggtatctt acctctcacc gagcatttat ccgaaaaaaa gttagctaaa
    19621 atttcaggaa acaaaaaggt acaaatgata cctcaaaaag atctccagaa aataacaggt
    19681 tatattcacg gagcaaataa tccaattgga attcgccaaa aacataatta ccctattttt
    19741 atcgatacta tcgctttaga aaaacaagaa ctcattgtct ctgcaggaga aattggacgt
    19801 tctattcgta ttaatagtga ggttcttgca gatttcgtaa atgctaaatt tgccgatata
    19861 aaggaataat aattaggatt ggagaaaaat atgaaatttt atcttgttag acacggaaaa
    19921 acacaatgga atttagaagg gcgtttccaa ggtgctaacg gagactcacc tctccttgaa
    19981 gaagctattg aagaattaga agaacttgga caatacctat catctattca ttttgatgct
    20041 gtttattcta gcgacttagg tcgtgctaga gatactgtta atatcctcaa tgatgctaat
    20101 tcatgcccaa aagaaatcca ctatacttct cagcttcgtg aatgggcatt gggaacttta
    20161 gagggttgta aaattgcaac tatgcaagct atctatcctc gtcaaatgac cgcattttat
    20221 caaaatccct tacaatttaa acatgacatg ttcggtgcag aatctcttta tcagacaacg
    20281 catcgtgtag agagtttctt acgttcttta gcttcaaaaa attacgacaa ggtccttatt
    20341 gtaggacatg gagcaaatct aactgcgtct attcgttcct tactgggtta tcaatatggt
    20401 agtttacact acaaagataa acttgataat gccagcctca caatcataga aactcatgat
    20461 tttaaagact tcaactgtct gacttggaac gataaatctt atttaaggca agaggtcaaa
    20521 atgacacact aaaataatcc actagattca tctagtggat tatttaatta tctagcatac
    20581 ttcatgcgta atactttttc ctgttttgca gttaggcgca gtaaaataac aactttgtca
    20641 acgctcatat tactctcaag cagacgctct gctgccatca ttaaaccatt attaagtccc
    20701 atttcaagga ctggattact tttatcgttg atatcaaaaa taaatttatt atcaggtaag
    20761 tcaaacaaca ctttaacagc accgaataat tgctcaaagt tatcaatagc aacatcataa
    20821 actggtttaa ttcccggttt taaagaacgt cctctatggt tgaaccacat agaatgccaa
    20881 ccaccattaa atgcacccat aatatcatta tcatatgaat cacccacata aagtgtagtt
    20941 gaagggttca tatcaaattg ctctgctgct aaattaaaaa tctccttttc aggcttttga
    21001 aaaccagttg cttgactaac tataacacgt tttggatcaa catagtcata aagtcccaat
    21061 tttttaactt ttttcaattg atgttccgta gggccatttg taataattcc cattggtaca
    21121 ttttttgatt tcaaaaagtc aagtgtcata cgcatctcat caagcattgt aatattttca
    21181 agttcatgtt cgtatatttc ttgaaaataa ataccagtag cttcatcaat ttcacgataa
    21241 ccaaactcta aaagcgtttc tttgcatcgc cagaaacgaa aatattctgt tgtccattca
    21301 ccagccatta ctcgtggaaa gccaatatct gaataatgac gaaagcgaat ataagcttgg
    21361 ttaatagcgc tcatatcaaa atcaggaaag catttctcca cagcaatacg gtaaggtgct
    21421 tgttgatcgt aaattgtatc atcaacatca aaaacaattg aagtaatcat gattatcctt
    21481 ttctttctga attttcaatt gttaattata cctttttcta cccttatttt caaatggtga
    21541 aaagtctatt aaaaatgaga ataacaccat aaactcataa aataaattat gaattatcag
    21601 tgtttttgtg gtacaatagg atgaatacta acttggagga aattatgtct aaccaacata
    21661 ttgaagaatt aaatgaccaa cagattgttc gtcgtgaaaa aatggctgct ttgactgaac
    21721 aaggcattga tccttttggt aaacgatttg aacgtacagc aacatctggt caattaaatg
    21781 aaaaatacgc tgacaaatca aaagaagact tgcacgacat tgaagaaact gctacaatcg
    21841 ctggtcgttt aatgactaag cgcggtaagg ggaaagttgg ctttgcgcat atccaagatc
    21901 gcgaaggtca aattcaaatt tatgtccgta aggattctgt tggcgaagaa aactacgaaa
    21961 tctttaaaaa agctgattta ggtgacttcc ttggtgttga agggcaagta atgcgtacag
    22021 atatgggaga actttctatc aaggctactc atattactca cctttcaaaa gcactccgtc
    22081 cacttcctga aaaattccac ggcctaactg atattgaaac aatctatcgt aaacgtcatc
    22141 ttgatttaat ctcaaatcgt gacagctttg accgttttgt aacacgctca aaaatcattt
    22201 cagaaattcg tcgctttatg gatagcaatg gtttcttaga ggttgaaaca cctgttcttc
    22261 ataatgaagc tggtggtgct tctgctcgcc catttatcac acatcacaat gcacaagaca
    22321 ttgacatggt tcttcgtatc gcaactgaat tgcatctaaa acgtcttatt gtaggtggta
    22381 tggagcgtgt ttatgaaatc ggacgtattt tccgtaatga agggatggat gcaacccaca
    22441 atccagagtt cacatctatt gaagcctacc aagcctatgc tgactaccag gatattatgg
    22501 acttgacaga aggtattatc cagcatgtta caaaaactgt aaaaggtgat ggaccaatca
    22561 attatcaagg cacagaaatt aaaattaatg aaccattcaa acgtgttcat atggttgatg
    22621 cggttaaaga aatcactgat attgacttct ggaaagaaat gactcttgaa gaagcacaag
    22681 ctttggcaca agaaaaaaat gttcctcttg aaaaacattt cacaacagtt ggtcacatca
    22741 ttaacgcctt cttcgaagaa ttcgttgaag acactttaat tcaaccaact tttgtctttg
    22801 gtcatccagt cgaagtgtct ccacttgcta agaaaaatga tacagaccct cgttttacag
    22861 accgtttcga actctttatc atgactaaag aatatgctaa tgcttttaca gaattaaatg
    22921 acccaattga tcagctatct cgctttgaag cacaagcatc tgctaaagaa cttggcgatg
    22981 atgaagcaac tggagttgat tacgattatg ttgaagcact tgaatatggt atgccaccta
    23041 ctggtggact aggtattggt atcgaccgtc tctgtatgct gttaactgac acaacaacta
    23101 tccgagacgt cctacttttc cctacaatga aataagaata tattaaaacc cacccttatt
    23161 taaggtgggt tttaatatta cctctactga actttgttag gtttctgatt tatcgctcac
    23221 gcaatttttt atcaaggttt accatttcaa tagccgaaag agcacaatca taacctttat
    23281 ttcctgcttt tgttcctgcc ctctcaatag cttgttcgat attatccgtt gttagaacac
    23341 caaagatatg agggatatct gactggctat ttaaatgacc aatacctttt gtcacctcat
    23401 tacaaacata atcataatga tcagtgctgc ctttaatgac taccccaaga cagataatag
    23461 catcataatc tttatataat gccatttttc tagccatata ggggatttca aaagcaccgg
    23521 gtacccatac aatatctata tcttcttctg aaacaccatg tctcaacagt ccatctactg
    23581 ctcctgataa taatttagat gtaatcaatt cattgaaacg tgagactacg attcctattt
    23641 tcatttcgtt tgccactaat tgtccttcaa taattgtcat tttttcttcc tcctttaatc
    23701 taaaagatgt cccatttttt cttttttagt ttttaaataa ttaaggtcat aagctgttgc
    23761 tgccacctgt aatggcactc tgtttttgac gcaaattcct tctgcttcaa gttgttggat
    23821 tttatctgga ttattggtta gtaagtttat agaatttata cctaaatctt ttaaaagttg
    23881 agcactaact ccatagtctc tctcatctcc ttcaaaacct aaagccaaat tagcttctaa
    23941 agtatctaag ccttcttctt gcaaatgata agcttttaac ttattaatca aaccaattcc
    24001 tcgaccttcc tgtctcatat aaagaaggac gccacgacct tcagcctcta tttgttggag
    24061 tgcattagcc agttgttccc cacaatcaca tctcaaggaa tgaaagacat cacctgtcaa
    24121 acattctgag tgtaaacgac ataaaacatc ttcaccacta ctaatatccc ctttaacaag
    24181 ggcaacatgt tccttatttg ccacaacttc actatatcca taagccttaa aatggccgaa
    24241 ttgcgttggt agctgaatct caatttctct aaccacccct ccctcttctt gacgtctata
    24301 gtcttgcaat tgttttatgg tagcgatagc taaatcgtgc ttttgagcaa atgctaataa
    24361 ctcatccttt ctcatcatac taccatcttc agccattatt tcacaacaaa gtccacattc
    24421 ctttaatcca gctaaacgac atagatcaac ggttgcttca gtatgtccat ttctagctaa
    24481 aacacctcct tccttagcta acaagggaaa taagtgccca ggacgtctaa agtctttagc
    24541 tttgctactg tcgttagcaa ccatcttagc tgtcaaagct ctatcttccg cagaaatccc
    24601 agtactagtt gatagatgat caatggatat ggtaaaagct gtttcatgat tatcagtatt
    24661 atggctagcc atttgagcta attctaactt ttcagcatag taattagaca gaggtaagca
    24721 aattaatcct ttcccaaatt tagccataaa attaatcgac tcttttgtca ccatttcagc
    24781 cgcacaaata aggtcccctt cattctctct attttcgtca tccatcaaaa ctaccatttc
    24841 ccctgatttg atgtcttgta aaagtttttt tattggacta aaggccattc gctttctcct
    24901 ctctcttagt agccattttc tgataaaaaa gaccaatcca ttactgatga agtgtgatta
    24961 gttctattag tcggtgctaa aaatttctca gcatatttgc ctaaaacatc cacttcgata
    25021 tttaataaat ccccaacctt ttttaaggat aaattagttt gagccatcgt gtggggaacg
    25081 accgaaactt caaaagaatg ctctgtaaat gataccaccg ttaggctaat cccatctaga
    25141 gccaccgaac ctttctctat aatataagac ataatactgg gcggagcatc aattgaaaat
    25201 ctgacagcaa tatcatcctt ttggattttt tctatttttc caaccccatc cacatgtcca
    25261 agaacaaaat gcccccctaa ccttgagtca cttctgagtg ctaattctaa gttaacttta
    25321 gaatgaagtc gtaaagcagc taagcttgtt cgcatcatac tttctggcat gacgtctgct
    25381 ataaaagctt tttcactttt gtcaaggact gtcaaacaaa caccattaac agctacgcta
    25441 tccccttttc ttaataaagg gactagtttg ggaacatcaa tacttaattg ttgggatttt
    25501 atgccattcc gaatacgact gacttgaccc atttcctcaa taattcctgt aaacataatg
    25561 ttctaccttt ccttcaataa caacgtcaga atccatacga ctaagttcaa tatcttttac
    25621 tctgaaagct tggttaagcc aatccaagcc ttctccagaa atagctgttt ttgctttcag
    25681 acctcctatt attttgggtg caataaaaac aattagtctg ttaacaatac cagcttctaa
    25741 agctgaaaaa tgtaaactac ttcctccttc aataagaagg ctatcaatcc cttcttgtcc
    25801 taaaatagtc attaatactt tcaaatccag ctttccatct tttcttggga ccttaatcaa
    25861 acggcaaccc atttctttta atgtttgttg ctgagcaaga ttatctgaac atgttgcaat
    25921 ccatgttggt attgttttag cgctcttaac taggtaagag tctaaaggta tcttcaactg
    25981 actatcacat acgatcctca caagcgcttc acctttagga atacggcaag ttaaacgtgg
    26041 attatcagct aatacagtat tgataccgac cataatagca ctacattttt gcctcaattt
    26101 ctgaacagat tgtcttgagt gctcattgga tatccactta gaatcgcccg tctttgtagc
    26161 tatcttccca tctaaagtca tagcatattt caaataaaca aaaggctgct tataggtcat
    26221 gtgaaaaata aatctttcat tcagagcatc gcattcttct cttaggatac caacttcaac
    26281 atttaaacct tcttttctta acaaggcaat cccttttcct gaaactaaag gatttggatc
    26341 taaacttcca actactactt ttttgatacc agatttaata agagcttcag tacaaggtgg
    26401 ttgcttaccc acatgacagc atggttctaa agtcacgtac agagtacttc cagagatatc
    26461 ctcatcagca tttttaatag cctgtctttc cgcatgtaaa tctccaaatc gtttatgata
    26521 acctttggaa attatacgat catctttaac aataacagca cctactaatg ggttgggagc
    26581 aacgaacccc atacctttct cagcctcttt caaggctaat gccatgtagt cttctttttt
    26641 catcatttcc tttttccttc taaaataaaa aaccttagct tctaaaatag aaaactaagg
    26701 ggagggatga gaagtcgttg attatactaa ttagcattat attttaggta gcacaaaaaa
    26761 actaaagact ccctatagaa aactataggg agtctctcat taatccagca caaataaact
    26821 gcaagtagca gacaatcact gaattcttct ctcatccaga ctttactgtc ggtactggaa
    26881 tcacaccagt tcatgctcga aagctcgcgg actataccgc cggttaggat tttcacctga
    26941 ccccgaagaa attctatttg acagttattt tactaaactc atttttattt gtcaataaaa
    27001 ataatgtgtt ttttattata aaataataat taagtagctc atctattctt atctttaaaa
    27061 gacagtatat ctataccata aagcaatctt tcgaactaat caaacaagaa tcgatagtat
    27121 tattggatta tatttcttaa aaaattcact acagatatag tttactccac ttaaataact
    27181 taggtaaatc tacggaacta aaaataatcg atagaaatct gttattttgt tatttcaaat
    27241 gtaactgtta agaaaat
//
LOCUS       DATROQ010000015        27040 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.015, whole genome shotgun sequence.
ACCESSION   DATROQ010000015 DATROQ010000000
VERSION     DATROQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27040
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.015"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            364..1726
                     /locus_tag="VBC94_000626"
                     /pseudo
     CDS             364..1726
                     /locus_tag="VBC94_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1898..2512)
                     /locus_tag="VBC94_000627"
     CDS             complement(1898..2512)
                     /locus_tag="VBC94_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO6978920.1"
                     /translation="MSFLEELKNRRSIYALGRNTEVSDEKIVEIIKEAVRQSPSAFNS
                     QTSRVVILLNDEVTKFWDELVANDLVETMKVQGAPETAIAGTKEKLASFGASKGTVLF
                     FEDQDVVKSLQEQFVLYADNFPVWSEQSTGIASVNTWTALSAELGLGGNLQHYNPVID
                     ASVQAVYGVPASWKLRGQLNFGSIEAETGEKEFMNDDDRFKVIG"
     gene            complement(2645..3070)
                     /locus_tag="VBC94_000628"
     CDS             complement(2645..3070)
                     /locus_tag="VBC94_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6978921.1"
                     /translation="MGNFKNSAVKSMVVMRKAFRTIDGKVSESFKEFELTPTQFAVLD
                     VLYAKGTMKIGELIENMLATSGNMTVVIKNMEKKGWVLRHSCPNDKRAFLVSLTTEGE
                     EVIKKALPEHIKRVEDAFSVLTETEQEDLINLLKKFKTL"
     gene            complement(3197..7603)
                     /locus_tag="VBC94_000629"
     CDS             complement(3197..7603)
                     /locus_tag="VBC94_000629"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEO6978922.1"
                     /translation="MSELFKKLMDQIEMPLEIKNSSVFSSADIIEVKVHSLSRLWEFH
                     FSFPELLPIEVYCELQTRLVNSFEKADIKATFDIRAETIDFSDDLLQDYYQQAFCEPL
                     CNSASFKSSFSQLKVHYNGSQMIISAPQFVNNNHFRQNHLPRLEQQFSLFGFGKLAID
                     MVSDEQMTQNLKSSFETNREQLLEKANQEAMQALEAQKSLEDSAPPSEEVTPTQNYDF
                     KERIKQRQAGFEKAEITPMIEVTTEENRIVFEGMVFSVERKTTRTGRHIINFKMTDYT
                     SSFAMQKWAKDDEELKKYDMISKGSWLRVRGNIENNNFTKSLTMNVQDIKEIVHHERK
                     DLMPADQKRVEFHAHTNMSTMDALPTVESLIDTAAKWGHPAIAITDHANVQSFPHGYH
                     RAKKAGIKAIFGLEANIVEDKVPISYNEVDMNLHEATYVVFDVETTGLSAANNDLIQI
                     AASKMFKGNIIEQFDEFIDPGHPLSDFTTELTGITDNHVRGSKPILQVLQEFQNFCQG
                     TVLVAHNATFDVGFMNANYERHNLPLITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQ
                     VALEHHHMANYDAEATGRLLFIFLKEARENRDVTNLMELNTKLVAEDSYKKARIKHAT
                     IYVQNQVGLKNIFKLVSLSNVKYFEGVARIPRSVLDAHREGLLLGTACSDGEVFDALL
                     SNGIDAAVTLAKYYDFIEVMPPAIYRPLVVRDLIKDEAGIQQIIRDLIEVGRRLDKPV
                     LATGNVHYIEPEDEIYREIIVRSLGQGAMINRTIGRGEDAQPAPLPKAHFRTTNEMLD
                     EFAFLGKDLAYEIVVTNTNTFADRFEDVEVVKGDLYTPFVDRAEERVAELTYAKAFEI
                     YGNPLPDIIDLRIEKELASILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVA
                     TMIGITEVNPMPPHYVCPNCQHSEFITDGSCGSGYDLPNKNCPKCGTLYKKDGQDIPF
                     ETFLGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGEEYAFRAGTVGTVAEKTAFGFVK
                     GYERDYNKFYNDAEVERLATGAAGVKRSTGQHPGGIVVIPNYMDVYDFTPVQYPADDM
                     TAAWQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPSNILPDDPDVMKLFS
                     GTEVLGVTEEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWL
                     GNAQDLIKEGIATLSTVIGCRDDIMVYLMHAGLQPKMAFTIMERVRKGLWLKISEDER
                     NGYIQAMRDNNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHYPIFYYCAYF
                     SIRAKAFELRTMSAGLDAVKARMKDITEKRQRNEATNVENDLFTTLELVNEMLERGFK
                     FGKLDLYRSHATDFIIEEDTLIPPFVAMEGLGENVAKQIVRAREDGEFLSKTELRKRG
                     GVSSTLVEKFDEMGILGNLPEDNQLSLFDDFF"
     gene            complement(7726..8310)
                     /locus_tag="VBC94_000630"
     CDS             complement(7726..8310)
                     /locus_tag="VBC94_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEO6978923.1"
                     /translation="MKSRKKDKLVLRLTTTLLVFGLGGVWFYNYKNDNVEPTVTSASD
                     QTTTFIQTISPTAIEISKTYDLYASVLLAQAILESSSGQSDLSKAPNYNLFGIKGEYK
                     GKSVQMPTLEDDGKGNMTQIQAPFRAYPNYSASLYDYAELVSSQKYASVWKSNTSSYK
                     DATAALTGLYATDTAYASKLNQIIETYSLDAYDK"
     gene            complement(8435..10288)
                     /locus_tag="VBC94_000631"
     CDS             complement(8435..10288)
                     /locus_tag="VBC94_000631"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEO6978924.1"
                     /translation="MKQSKMLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMRQEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDQS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHKDYEDLDVTYEDYRKAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAVTPNRT
                     DLNRWLVLDKTIPSIDDIPEDVLEEIKAELSAWLVSGEDTIAYSTESSYAANLEMATN
                     EYKPSTKAATFEEVTKVETPNCKSIDEVAGFLSIDENQTIKTLLFIADEQPVVALLVG
                     NDQVNDVKLKNYLAADFLEPASEEQAKEIFGAGFGSLGPVNLPESVKIIADRKVQDLA
                     NAVSGANQDGYHFTGVNPERDFTAEYVDIREVKEGEISPDGKGTLKFARGIEIGHIFK
                     LGTRYSDSMGANILDENGRSNPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGAYRF
                     AWGINFPEELAPFDVHLITVNVKDQESQDLTEKIEADLMLKGYEVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKASEGIVEVKIKASGDTIEVHADNLIETLEILTKK"
     gene            complement(10380..11639)
                     /gene="rseP"
                     /locus_tag="VBC94_000632"
     CDS             complement(10380..11639)
                     /gene="rseP"
                     /locus_tag="VBC94_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEO6978925.1"
                     /translation="MLGILTFIIIFGVIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHIDKEGTTYTIRILPLGGYVRMAGWGDDKTEIKTGTPASLTLNKEGIVTRINLSGK
                     QLDNTSLPINVTAYDLEDKLTITGLVLSETKTYSVDHDATIIEEDGTEIRIAPLDVQY
                     QNASVWGRLITNFAGPMNNFILGLVVFIALAFIQGGVQDLSTNQVRVSENGPAASAGL
                     KNNDRILQIGSHKVSNWEQLTAAVEKSTSHLEKNQKLALKIKSKEVVKTINVKPQKVD
                     KSYIIGIMPALKTSFKDKLLGGFKLAWESFFRILNELKKLIAHFSINKLGGPVALYQA
                     SSQAAKNGFVTVLNLMGLISINLGIMNLIPIPALDGGKIVMNILEAIRRKPLKQETET
                     YITLAGVAVMLVLMIAVTWNDIMRAFF"
     gene            complement(11670..12464)
                     /locus_tag="VBC94_000633"
     CDS             complement(11670..12464)
                     /locus_tag="VBC94_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEO6978926.1"
                     /translation="MKERVIWGAVALAIFIPFLVMGGLPFQFLVGLLAMIGVSELLRM
                     RRLEIFSFEGALAMIGAFVLTVPLDSYLSFLPVDASLSAYGIVIFMILAGTVLNSNSY
                     SFEDAAFPIASSFYVGIGFQNLVSARMAGIDKVLLALFIVWATDIGAYMIGRQFGQRK
                     LLPSVSPNKTIEGSLGGIASAIVVAFFFMLFDKTVYAPHSFLVMLVLVAIFSIFGQFG
                     DLVESSIKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHFFGLF"
     gene            complement(12479..13231)
                     /locus_tag="VBC94_000634"
     CDS             complement(12479..13231)
                     /locus_tag="VBC94_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEO6978927.1"
                     /translation="MFIFKRKKQVEMPLEKIPAHIGIIMDGNGRWAKKRLKPRVMGHK
                     AGMDALQEVTIAASELGVKVLTVYAFSTENWSRPDDEVKFIMNLPVEFFDKYVPELDK
                     NNVRVQVIGDTHKLPKATYDAMQRACLRTKHNSGLVLNFALNYGGRSEITNAIKEIAQ
                     DVLEAKLNPDDITEDLVANHLMTNSLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEF
                     YFTPVLWPDFKKDELHKAIVDYNQRHRRFGSV"
     gene            complement(13398..13739)
                     /gene="yajC"
                     /locus_tag="VBC94_000635"
     CDS             complement(13398..13739)
                     /gene="yajC"
                     /locus_tag="VBC94_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEO6978928.1"
                     /translation="MSLPIIMLVVMVGMMFFMQRQQKKQAQERQKQLNAVQKGDEIVT
                     IGGLFGVVDEVNTEAQRMVLDVDGVYLTFELAAIKSVVSKAATPTEPVEDVKIIEVNE
                     DENKETAIETE"
     gene            complement(13816..14160)
                     /locus_tag="VBC94_000636"
     CDS             complement(13816..14160)
                     /locus_tag="VBC94_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEO6978929.1"
                     /translation="MYSFEELLATMTLITAAEIEDKIDSNQDFVLFIGRISCPFCHLF
                     VPKIVEVADEDEFELFHLDSEDFDHWTANKDFRNKYDIPTVPGLMVVKNGTIKVKCDS
                     KMTKEEIREFIG"
     gene            complement(14351..15514)
                     /locus_tag="VBC94_000637"
     CDS             complement(14351..15514)
                     /locus_tag="VBC94_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEO6978930.1"
                     /translation="MSENLGQLAINQARENGGKLEVISKVKVEDKRDLSIAYTPGVAS
                     VSSAIAEDVELAYELTTKKNTVAVVSDGSAVLGLGDICPEAAMPVMEGKAALFKRFAN
                     VDAVPIVLKTNDTEEIISIVKAISPTFGGINLEDISAPRCFEIEQRLIEECDIPVFHD
                     DQHGTAIVVLAALFNSLKLVKKDIEDIKVVVNGGGSAGLSITRKLLSAGAKHVTVVDR
                     FGIINDKDRESLAPHHKAIAKLTNREFQSGSLEDALENADVFIGVSAPEALHAEWISK
                     MADKPIVFAMANPIPEIYPDQALKAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAARGIASLIPEEELSTTHIIPNAFQNDVADVVAKSVSNAVQK"
     gene            complement(15539..16876)
                     /locus_tag="VBC94_000638"
     CDS             complement(15539..16876)
                     /locus_tag="VBC94_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEO6978931.1"
                     /translation="MADVKVVNNEGSRGQKQDLKTKLFHIKVGSVPLPVYVCLALLIL
                     LAGFLQKLPVNMLGGFAVILTMGWFLGTIGASIPGFKNFGGPAILSLLVPSILVFFNL
                     INKNVLESTNMLMKQANFLYFYIACLVSGSILGMNRKMLIQGLLRMIFPMLLGMVCAM
                     MVGTFVGVILGLEWRHTLFYIVTPVLAGGIGEGILPLSLGYSSITGVASEQLVAQLIP
                     ATIIGNFFAILCTALLNRLGEKKPHLSGQGQLVRLNKGEDMSDIIADHSGPIDVKKMG
                     GGVLTACSLFIFGHLLQQLTGFPGPVLMIIAAAILKYINVIPRETQNGAKQLYKFISG
                     NFTFPLMAGLGLLYIPLKDVVATLSIQYFIVVISVVFTVISVGFFVSRFLNMNPVEAG
                     IISACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRMLF"
     gene            17030..18556
                     /locus_tag="VBC94_000639"
     CDS             17030..18556
                     /locus_tag="VBC94_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6978932.1"
                     /translation="MKKKLSLWAFLSLILVTMTICIFSIFYYVTIHQSYRMVRVQEEK
                     ILKNTGYALSRNPQVIQTLKDNHYNQSLQKQMLFLSKKSNLDYIVLINLKGIRFTHPD
                     STKIGKPFQGGDEQAVFKGKAIMSTAEGSLGKSLRYLIPVYDHQKQVGAIAVGLKLTT
                     LGDLSQSSIKEFSKPLLISILISLVVTSIISYGLKKQLHNLHPSDIFQHLEERNATLD
                     QIQAAVFVIDQRHIIKRNNPAASLLFKKEGQRDLFSGKLLESLIPQLKQDHFSKKTEQ
                     VLHFQGQDYLLSISPITVKTQNRGYVVFLRNVTETLFTLDQLAHTTAYASALQAQTHQ
                     FMNQLHVIYGLADIEYYDELKIYLKELLEPQNEFLARLSMLVREPRLASFIIGEREKF
                     AEKHINLSTEILVEIPTKSTVEDVNNYLLLHRYINTKILTLLNSTTLVSLRLNYQNNL
                     IETDYQWENEKWLLNDYHQYFNDAYFQQLLVDSRATYKISLKQNKVIIRTNIIYSEGS
                     "
     gene            18558..19238
                     /locus_tag="VBC94_000640"
     CDS             18558..19238
                     /locus_tag="VBC94_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO6978933.1"
                     /translation="MDVLIIEDDPMVEFIHRNYLEKLNYFQNIYSTASQTQAIAYLND
                     IKIQLVLLDIHIKEGNGLELLKLLRNQHQNTEVIVISAANEAHTVKEAFHLGIVDYLI
                     KPFTFERFESSIEKFLNHYHTFEADKIYQDNIDHFQKIDSGWLEGEVKLDEKGLSEIT
                     YQHILDAIQELEQPFTIQELAKCSQFSHVSVRKYIAYMEEKGLLTSQQIYTKVGRPYK
                     VYKLIKKT"
     gene            complement(19259..20254)
                     /gene="galE"
                     /locus_tag="VBC94_000641"
     CDS             complement(19259..20254)
                     /gene="galE"
                     /locus_tag="VBC94_000641"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEO6978934.1"
                     /translation="MAVLILGGAGYIGSHMVDQLITQGKEKVIVVDNLVTGHRQAVHS
                     DAIFYEGDLSDKTFMRQVFRENPDVDAVIHFAAFSLVAESMENPLKYFDNNTAGMIKL
                     LEVMNECDIKNIVFSSTAATYGIPEQVPILETAPQNPINPYGESKLMMETIMKWADQA
                     YGIKFVALRYFNVAGDKPDGSIGEDHKPETHLLPIILQVAQGVRDKIMIFGDDYNTPD
                     GTNVRDYVHPFDLADAHILAVDYLRQGNESNVFNLGSSTGFSNLQMLEAARRITGKEI
                     PAQKAARRPGDPDTLIASSEKARQMLGWEPKFDNIDKIISSAWAWHSSHPNGYED"
     gene            complement(20342..21949)
                     /locus_tag="VBC94_000642"
     CDS             complement(20342..21949)
                     /locus_tag="VBC94_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEO6978935.1"
                     /translation="MKKHWWHKATIYQIYPRSFMDSDGDGVGDIKGITSKLDYLEKLG
                     ITAIWLSPVYQSPMDDNGYDISDYQAIADIFGDMNDMDQLLQEANQRGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDKASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIDKGIGGFRMDVIDLIGKIPDKGITGNGPKLHDYL
                     KEMNRASFGKHDLLTVGETWGATPDIAKQYSNPDNEELSMVFQFEHVGLQHKPDAPKW
                     DYSDGLDVPALKDIFTKWQTQLELGQGWNSLFWNNHDLPRVLSIWGNDSDNRKQSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFECLADVDDIESLNYAKEAMDNGVSEATILDS
                     IRKVGRDNARTPMQWSQGHQAGFTNGTPWLAVNPNYQEINVEAALNDTESIFYTYQKL
                     VALRKEHDWLVDADFKLLETADKVFAYVRQTDKERYLIVANLSDQNQSFEFPEAVKET
                     IISNTEVQEVLSSNTLKPWDAFCIELF"
     gene            complement(22078..23211)
                     /gene="ugpC"
                     /locus_tag="VBC94_000643"
     CDS             complement(22078..23211)
                     /gene="ugpC"
                     /locus_tag="VBC94_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEO6978936.1"
                     /translation="MVELNLNHIYKKYPSASHYSVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIDGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKFSKQEIDKRVREAAANIGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHQRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPDGD
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPSMNFFKVKVENGMIISEDGLRIAIPEG
                     QEKLLESRGYKGKELIFGIRPEDISSNLLVQDTYPNANVEAEVLVSELLGSETMLYVK
                     LGQTEFASRVEARDFHNPGEKVNLTFNVAKGHFFDADTEQAIR"
     gene            complement(23312..24163)
                     /locus_tag="VBC94_000644"
     CDS             complement(23312..24163)
                     /locus_tag="VBC94_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6978937.1"
                     /translation="MDFKELFPEVITKQEVKQSEDYIIVEQDGHVLHFPKSSLTKREL
                     CLLQMTQSLEEASSVDSQNPWYRYLVEGRGRLPQSYSAVQFIFIEHQFTLSEELKDFL
                     SPLVINVETIMTINQTQSVMILNQDNFFNATELLTDILPTIENDFNTRLRCYFGNSWT
                     HLQAVDWKELYEEEYKLFTLFLSHKAEQHYCRFPKMALWALANQSPMPSIKAKCLQHI
                     LDTSDTSAIIKALWQEQGNLAKTAKALFIHRNSLQYKLDKFTQSSGLNLKILDDLAYA
                     YLISMDT"
     gene            complement(24258..25154)
                     /locus_tag="VBC94_000645"
     CDS             complement(24258..25154)
                     /locus_tag="VBC94_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEO6978938.1"
                     /translation="MAITIQNHELQVTLKALGATMTSITDSQGVEYLWQGEPTYWGGQ
                     APILFPICGSVRNDCVIYRPAQAPHFTGIIPRHGFVRHKTFDYDYISDSSVRFTIKSS
                     EETLINYPYRFSLEITYTLRNKSIAITYIVKNLESEKNMPYAIGAHPGFNCPLFEKEV
                     FSDYYLEFEQFETCTIPESFPDTGLLDLQARHPFLENQKQLSLNYALFEKDAITLDQL
                     RSKTVYLKSRNHAKGIQLDFDDFENLILWTSNNGGPFLALEPWSSLSTSIEESDILED
                     KQNIVRLNPKQSKQHSIRITIL"
     gene            complement(25208..26185)
                     /gene="lacD"
                     /locus_tag="VBC94_000646"
     CDS             complement(25208..26185)
                     /gene="lacD"
                     /locus_tag="VBC94_000646"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEO6978939.1"
                     /translation="MGLTEQKQKHMEQLSDKNGIISALAFDQRGALKRLMAKYQSEEP
                     TVSQIEALKVLVAEELTPYASSMLLDPEYGLPATKVLDDNAGLLLAYEKTGYDTSSTK
                     RLPDCLDIWSAKRIKEEGADAVKFLLYYDVDSSDEVNEEKEAYIERIGSECVAEDIPF
                     FLEILSYDEKITDSSGIEYAKIKPRKVIEAMKVFSNPRFNIDVLKVEVPVNMDYVEGF
                     AQGETAYNKATAAAYFREQDQATLLPYIFLSAGVPAQLFQETLVFAKEAGAKFNGVLC
                     GRATWAGSVKEYVEKGEAGARQWLRTIGFQNIDELNKILQKTATSWKER"
     gene            complement(26187..>27040)
                     /locus_tag="VBC94_000647"
     CDS             complement(26187..>27040)
                     /locus_tag="VBC94_000647"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEO6978940.1"
                     /translation="VTDVSKTPGGKGLNVTRVLSQLGDNVVATGLLGGHFGDFIRSGL
                     DALEIRHQFLSIGGETRHCIAVLHEGQQTEILEKGPHITKDEAGAFLNHLKLIFDAAT
                     IITVSGSLPKGLPSDYYARLISLANHFNKKVVLDCSGEALRSVLKSSAKPTVIKPNLE
                     ELTQLIGRPISYSLDELKSTLQQDIFRGIDWVIVSLGARGAFAKHGNHYYQVTIPKIE
                     VINPVGSGDATVAGIASALEHQLDDINLLKRANVLGMLNAQETLTGHINLTYYQELIS
                     QIQVKEV"
BASE COUNT         8842 a         5390 c         4295 g         8513 t
ORIGIN      
        1 aaaaacagtg aagataagtt tattcaagag ttaaataggt ataaaacaga gataccaaat
       61 ttcaaaggtt ttaatgtttg gattttaggt gataaaggtt attacaaaaa ccttatcaat
      121 ttagaagaaa ttaaaaatat ccaagctaca ttaaaaaaag aaagaaatga agaatatgtc
      181 tttgtcaaat taaatgggaa aatcgcacat gatacaacag tattcttgat gaataaaaaa
      241 cataaattac ttaaaaatat agaggaattt aagacgataa cccaaaaaag gctaactgag
      301 agaggaaagt tcccatatga taccgttcat tctacgtttg aaattaagga tgaaaacttt
      361 atcatggaaa gattgaaatc atcagggtta tctatgggta aaccagttga ttatatgggc
      421 gtaaatggta tccctattta cactaaaaca ttatctatag ataataagtt tgcctttgaa
      481 aataattcaa aagattcttc atattcttca aacattaata ttagtgaaga taaaataaaa
      541 gaaaatgacc aaaaaattct agatttaata gttaaatctg gagctaataa tcaaaactta
      601 acagatgaag aaaaagtaat tgctttcact aaatatatag gtgaaataac taattatgac
      661 aatgaagctt acagagctag aaatgtcgac acagaatatt acagagcttc agatttattt
      721 tctgtaacag aaagaaaact tgctatgtgc gttggctata gtgtaacagc cgctagagca
      781 tttaatatta tggggatacc tagttatgtc gtttctggta aaagtcctca aggaatatct
      841 catgcagctg tgagagcgta ttataataga agttggcata tcatagatat cacagccagt
      901 acatattgga aaaatggtaa ttacaaaaca acgtattccg attttattaa agaatactgt
      961 atagatggtt atgacgttta tgatcccgca aaaacaaata atagatttaa agttaagtat
     1021 atggaatcaa atgaagcgtt tgaaaattgg atacataata atggaagcaa atctatgtta
     1081 tttattaatg aatctgcagc tctaaaagat aaaaaaccaa aagatgattt tgtaccagta
     1141 acagaaaaag aaaaaaacga gctgatagat aagtataaaa aattattgtc acagattcct
     1201 gaaaacacac aaaatccagg agaaaaaaac ataagagatt atttgaaaaa tgaatatgaa
     1261 gaaatattaa agaaagataa tttatttgag catgaacatg cagaatttaa agaaagtcta
     1321 aatttaaatg aatcattcta cctacaatta aagaaagaag aaaagaaacc gagtgataat
     1381 ctcaaaaaag aagaaaaacc aagagaaaat tcagtaaaag agagagagac tccagctgaa
     1441 aacaatgatt ttgtttcagt aacagaaaaa aacaacttga ttgataaata taaagaatta
     1501 ttatcaaaaa tcccagagaa tacacaaaat cctgggggaa aagaatatac gaaattatct
     1561 agagaaagaa tatgaagaac tactacaaaa agatagatta ttcaagcatg agtacacaga
     1621 gtttacaaaa agtctaaatt tgaatgaaac attctattca caattaaaag aaggagaaat
     1681 gaaactaagt gaaaatcccg aaaaaggaga aacgaacacg aattaacctt tcaaatatct
     1741 acatcaaata acagaagtct ccgtatcaag cgaattgtag cttttttgaa tatactaaaa
     1801 acactcctac ctctatttta ctagaagtag gagtgttttt ttcattttag ttcatagatg
     1861 cagtgccatt gctatccttg cctttttttg tacagtatta tccgatgact ttaaagcggt
     1921 catcatcatt catgaattct ttttctcctg tttcagcttc gattgaacca aaatttagtt
     1981 gtccacgaag tttccaacta gctggaacac cataaacagc ttgtactgat gcatcaataa
     2041 ctggattgta atgttgtaaa ttaccaccaa gtccaagttc tgcagaaagg gctgtccaag
     2101 tatttactga ggcaatacct gttgattgct cagaccaaac tggaaagtta tccgcataaa
     2161 gaacgaattg ttcttgaaga gatttgacaa catcttgatc ttcaaagaag agtactgttc
     2221 cctttgaagc tccaaaacta gctaattttt ccttagttcc agcaatagct gtctctggag
     2281 caccttgtac tttcattgtt tctaccaaat cattagcaac caattcatcc cagaacttag
     2341 taacttcatc attcaataaa atgacaacac gggaagtttg actattaaaa gcagatgggg
     2401 attggcgaac tgcctcttta ataatctcta ctattttctc atcagaaact tctgtattgc
     2461 gaccaagagc gtaaatagag cgacggtttt ttaattcttc caaaaatgac atattttttc
     2521 tccttgttct tcaatctcag atatcttact acatttgttc tagtgacata tgaattagat
     2581 taaagttatt tattactatg tagtaattat attaccacta tatcgtaaag tcaaagaatg
     2641 tgactcacaa ggttttaaat ttttttaaaa ggttaatcaa gtcttcttgc tctgtttctg
     2701 ttaagactga aaaagcatct tctacacgtt taatatgctc tggtagagct ttttttatca
     2761 cttcttctcc ttcagttgtt aaactcacca aaaaggctct cttatcattc ggacaagaat
     2821 ggcgtaatac ccatcccttc ttttccatat ttttaataac aacagtcata ttacctgatg
     2881 tagcaagcat attttcaatc aattctccaa ttttcattgt gcctttagca tagagaacat
     2941 ccaaaactgc aaactgagtt ggtgttaatt caaattcttt aaatgattct gagacttttc
     3001 cgtctattgt tctaaatgct tttcgcatga caaccataga tttaaccgct gaatttttaa
     3061 agtttcccat ttcatctccc aaacgtattt tttcttattt tatcactaat tagttatttt
     3121 aagcaagaat atcatctttg ccctatcaaa agcagctggc aaataataag gttgagtaac
     3181 tactcaacct tattgcttag aagaaatcat caaaaagact caactgattg tcttctggta
     3241 aatttcccaa aatacccatc tcatcaaatt tttcaactaa ggtacttgag actcctcctc
     3301 gtttacgaag ctctgttttt gataaaaatt caccatcttc acgtgctcga actatctgtt
     3361 ttgcaacgtt ttctcccaag ccttccatag caacaaatgg agggattagc gtatcctctt
     3421 caataataaa gtcagtcgca tggctacgat aaagatctaa tttcccaaat ttaaagccac
     3481 gctcaagcat ttcattaacc aactctaatg tcgtaaaaag atcattttca acattcgtcg
     3541 cctcattacg ctgacgcttc tcagtgatat ccttcatgcg cgccttaacg gcatcaagtc
     3601 cagcactcat tgtgcgtaat tcaaaagctt tggcgcgaat agaaaaataa gcacaataat
     3661 aaaatattgg ataatgtact ttaaagtacg ccacacgtaa ggccattaaa acataggctg
     3721 ctgcatgggc tttagggaac atatacttga tttttccaca cgattcaata taccaatccg
     3781 ggacattatt atcacgcatt gcttggatat aaccgttacg ctcatcttct gaaattttca
     3841 accataaccc cttacgcacg cgctccatga ttgtaaaggc catctttggt tgtagaccag
     3901 cgtgcattaa ataaaccatg atgtcatcac gacaaccaat aacggttgat aaggtagcaa
     3961 taccttcttt gattaagtct tgagcattac ctaaccaaac atccgtacca tgtgatagtc
     4021 ctgacagttg caacaattct gcaaatgtgg ttggatgggt ttcgttcacc ataccacgaa
     4081 caaagttcgt accaaactca ggaataccaa gcatccctgt aggagttcca atttgctctt
     4141 ctgtcactcc taaaacttcc gtaccagaaa ataacttcat aacgtctgga tcatctggaa
     4201 ggatatttga tggatcaatt cctgataaat cctgaagttt acgaatcata gtaggatcat
     4261 catgtcctag aatatctaac ttcaagacat tctcatcaat atcatggaaa ttgaagtgag
     4321 ttgtctgcca agcagctgtc atatcatctg ctggatattg aaccggagta aaatcataaa
     4381 catccatata gttaggaata acaacgatac caccggggtg ttgacctgta gaacgtttga
     4441 ctcccgcagc accagtcgct aaccgttcga cctctgcatc gttataaaat ttattgtaat
     4501 ctcgttcgta acctttgaca aaaccaaagg cagttttttc agctacggta cctaccgttc
     4561 ctgcacgaaa agcatattct tctccaaaga tatcacgaac atctaagtga gcagaaggtt
     4621 gatcatctcc agagaaattt agatcaatat caggaacctt atctccatca aatcctaaga
     4681 aagtttcaaa cggaatatcc tgtccatctt tcttgtacaa agtaccacat ttaggacaat
     4741 ttttatttgg taaatcgtaa cctgaaccac aagaaccatc tgtgataaat tcagaatgct
     4801 gacaatttgg gcaaacataa tgaggtggca ttggattaac ttcagtaatt cctatcatgg
     4861 tcgctacaaa actcgaccca acagacccac gtgatcctac tagataacca cgttcattag
     4921 aacgttgcac caacatttgc gaagcaagat agataacggc aaatccatta ccaagtatag
     4981 atgctaactc tttttcgata cgtaaatcaa taatatcagg aagaggatta ccatatattt
     5041 cgaatgcttt agcataagtt aattcagcaa ctcgttcttc agcacgatca acaaacgggg
     5101 tatagagatc cccttttaca acttcaacgt cttcaaatcg atctgcaaaa gtattcgtat
     5161 tggttacaac gatctcatag gctaaatctt taccaagaaa ggcaaattca tctaacatct
     5221 catttgttgt acggaaatgt gcttttggca aaggcgctgg ttgagcatcc tccccacgac
     5281 cgatagtcct attaatcatg gcaccttgtc caagactacg aacaataatc tcacggtaaa
     5341 tctcatcttc aggctcaata taatgaacat ttcccgtagc taaaacaggc ttatctaaac
     5401 gccgaccgac ctctattagg tctctaataa tttgttgaat ccccgcctca tctttaatta
     5461 aatcacgaac caccaagggg cgataaatag caggtggcat gacttcaata aaatcataat
     5521 atttagctag ggtcactgct gcatcaattc catttgataa caaggcatca aaaacttctc
     5581 catcagaaca ggcagtacct agcaagagac cttctctgtg agcatctaaa actgatctag
     5641 gaattcttgc caccccttca aaatatttaa catttgaaag gctaactaac ttaaatatat
     5701 ttttaagtcc aacctgattt tggacataaa tggttgcatg ctttatgcgt gcctttttgt
     5761 aagagtcttc agctaccaat ttagtattga gctccatcaa attagtcaca tcacgatttt
     5821 cccgcgcctc ttttaagaaa ataaagagta agcgacccgt tgcttcagca tcgtagttgg
     5881 ccatatgatg atgttccaaa gcaacttgga aacgtttggt tagaggcccc aaaccatgac
     5941 gcttatattc aggataaaga tttcttgcaa attctaaggt atcaatgacc ggctgtgtga
     6001 tcaaaggcag attatgacgt tcataattcg cattcataaa accaacgtca aaggttgcat
     6061 tatgcgcaac taaaaccgta ccttgacaaa aattctgaaa ttcctgaagg acttgtaaaa
     6121 taggttttga acctctgaca tgattatccg taatacctgt caactcagtt gtaaagtcag
     6181 aaagaggatg gcctgggtca ataaactcat caaattgctc aataatatta cccttgaaca
     6241 tttttgatgc tgcaatctga attaggtcat tattagctgc agaaagacct gtagtttcca
     6301 catcaaatac aacataagtt gcttcgtgca aattcatatc aacttcgttg taggaaatag
     6361 gcactttatc ttcaacaata tttgcctcta atccaaaaat tgcttttata cctgcttttt
     6421 ttgctctgtg gtagccgtgc ggaaaacttt gaacattggc atggtcagta attgcaatag
     6481 ctgggtgtcc ccacttagct gcagtatcaa tgaggctctc gacagttggt aaagcatcca
     6541 tcgttgacat attggtatga gcatgaaatt ccacacgttt ttgatctgct ggcattaagt
     6601 ctttacgttc atgatgcaca atctctttga tatcttgaac attcattgtc aaagatttag
     6661 taaagttatt gttctcaata ttccctctga cccgcaacca cgaaccttta gaaatcatat
     6721 cgtacttctt caattcttca tcatctttag cccacttttg catagcaaaa cttgaggtat
     6781 aatcagtcat cttaaaatta atgatatgac gtccagtgcg ggttgttttt cgttcaacac
     6841 taaaaaccat tccttcaaaa acgatacgat tttcttctgt ggtcacctca atcatgggtg
     6901 taatctctgc tttttcaaag ccagcttgac gttgtttgat acgctctttg aaatcataat
     6961 tttgagttgg tgtcacttct tctgatggag gcgctgaatc ttctagtgat ttttgtgctt
     7021 ccaaggcttg catagcctct tggttagctt tttcaagcaa ttgctcgcgg ttagtttcaa
     7081 atgatgattt taagttttgc gtcatttgtt catcagaaac catatcaata gcaagtttac
     7141 caaatccaaa caatgaaaat tgttgttcca agcgcggcaa atgattctga cgaaaatgat
     7201 tattattgac aaattgaggt gctgaaataa tcatctgact gccattgtaa tgaactttta
     7261 attgtgaaaa agaactctta aaacttgcac tattacacaa gggttcacaa aaagcttgtt
     7321 gataataatc ctgtagcaga tcatcagaaa aatcaatagt ttctgctctt atatcaaaag
     7381 tagcctttat atctgctttt tcaaaactat tcactaaacg agtttgtaat tcacaataca
     7441 cctcaattgg caataattct ggaaatgaaa aatgaaattc ccataagcgt gatagagaat
     7501 gaacctttac ctcgataata tcagcagacg aaaaaacact tgaattctta atttcaagcg
     7561 gcatttcaat ctgatccata agtttcttga acaattctga catagttttc tcctgaatat
     7621 cttacttcca ttttatcaca tatctgtaaa tatttcgaga taacaaaagg aggctgttaa
     7681 aaaaggcatt tattagcctt cttaacaaca ctctcttaac tgagactatt tatcataagc
     7741 atctagactg taggtttcaa taatttggtt taatttacta gcataagcag tatctgtcgc
     7801 ataaagacct gttagagctg cagtagcatc cttataagaa gaagtatttg atttccaaac
     7861 agatgcatac ttttgactag atactaactc agcataatca tatagtgaag cagaataatt
     7921 tggataggcg cgaaaaggag cttggatttg agtcatattg cctttcccat catcttctaa
     7981 agtaggcatt tggacagatt tacctttata ttctcctttg atgccaaaga ggttataatt
     8041 aggagcctta gacaaatctg attgtccact ggatgattcc aaaatagctt gtgctaataa
     8101 gactgacgca tacaaatcat aggtcttaga aatttcaata gctgttggag aaatcgtttg
     8161 aataaaagtc gtcgtttgat ccgatgcact agtgactgtc ggttcgacat tatcattttt
     8221 ataattgtaa aaccaaaccc cacccaaacc aaaaactaat agtgttgttg ttaacctcaa
     8281 taccaattta tctttttttc gacttttcat gatggctcct ttctaaaata tcatttcatt
     8341 ataacaaaag gcagttgctt tttaccaata agaaccatac taaatttcac tccatataaa
     8401 aaagttagca catcagacta acttttttat cggcttattt ttttgttaga atctctaaag
     8461 tttcaattaa gttatcagcg tgaacctcaa tcgtatcacc acttgcttta attttcactt
     8521 caacgatacc ttcagaagct ttcttcccaa cagttactcg aattggaagt ccaattaagt
     8581 cactgtctga gaatttagaa ccaacacgtt cattacgatc gtctgtcagg acttcataac
     8641 ctttaagcat caaatcagct tcaattttct cagtaagatc ttgtgactct tgatctttga
     8701 cattgacagt aatcaaatgt acatcaaatg gggctaattc ttctgggaaa ttaatccccc
     8761 aagcaaagcg ataagctcct tttggtgtct tattcacaaa aagtcgggca tgttgttcaa
     8821 tgacagctga gagaatacga ctaacaccaa taccataaca ccccataaca ataggatttg
     8881 agcggccatt ttcatcaagg atgtttgctc ccatactatc tgaataacgg gtacctagtt
     8941 taaagatatg tccaatttca ataccacgag caaatttcag agtaccttta ccgtctggag
     9001 aaatctcccc ttcttttact tcacgaatat caacgtattc agctgtgaaa tcacgttctg
     9061 gattaactcc agtaaaatga taaccatctt ggttagcacc agacacagca ttagcaaggt
     9121 cttgaacttt acgatcagca ataattttaa cagactcagg taggttaact ggcccaagag
     9181 aaccaaaacc tgcaccaaaa atctcttttg cttgctcttc acttgcaggc tctaagaaat
     9241 cagccgctaa ataatttttt aacttcacat cattgacttg atcgttacca actagtaaag
     9301 caaccacggg ctgttcatca gcaataaaca atagtgtctt gatagtttga ttttcatcaa
     9361 ttgataaaaa tccagctacc tcatcaattg atttgcagtt aggagtttct acttttgtta
     9421 cttcttcaaa ggtagcagcc ttagtagatg gtttgtactc attcgttgcc atctcaaggt
     9481 tagcagcata gcttgattca gtggagtatg caatagtgtc ctcaccagaa actagccaag
     9541 ccgagagttc tgccttaatt tcttctaaaa catcctctgg aatatcatca atagaaggaa
     9601 tagttttatc aaggactaac caacgattta aatctgtacg attgggagtt actgccataa
     9661 attcttggct atctttacct cccatagcac ctccgtcacc tataatgcct ttaaagtcaa
     9721 gtccagcacg tgtgaagatg gcttcatatg ctttacggta atcttcgtaa gtgacatcaa
     9781 ggtcttcata atctttgtgg aagctgtatc catctttcat gataaattca cgtgttcgaa
     9841 gaagtccatt acgaggacgc ttctcatcac gatatttaga ctgaatttga taaaggttta
     9901 gtggtaattg cttataagat tttactgcat cacgaacaag agttgtgaaa gtctcttcat
     9961 gagttggtcc taagataaaa tccgattgat cacgattctt taatttataa aggtcttcac
    10021 catatgtttc gtagcgccca gattcacgcc ataaatctgc tgtaagaaga gctggagcta
    10081 acatttcaac tgcaccaatc ttctcaaatt cttgacgcat aattgtttta aatttttcaa
    10141 tagtacggtt agctagaggc aaataagcat agattccagc cgaaacttgg cggacatatc
    10201 ctgcacgaac cataagagca tggctgataa cctgagcatc acttggcatt tcgcgaagtg
    10261 ttggaataag cattttactt tgtttcatta caataagata cgactaatca aagttgattc
    10321 tttttcaatt gagaattgca attgaaaaat tcgtatttcc tttcacgttt tattatcttt
    10381 tagaagaaag ctcgcatgat atcgttccag gttacagcaa tcattaatac taacataact
    10441 gctaccccag cgagcgtaat ataagtttct gtttcttgtt taagtggttt gcgacgaatg
    10501 gcttctaaaa tattcatcac tatcttaccc ccgtcaaggg ctggtatagg gatgagattc
    10561 ataataccta agttgataga gattaatccc attaagttga gaactgtgac gaaaccattt
    10621 ttagcagctt ggctactagc ctgatagaga gctactggac ctcccaattt attaatacta
    10681 aaatgagcaa taagtttctt taattcattt aaaattctaa agaaactttc ccatgctagt
    10741 ttaaatccac ctaaaagctt gtctttaaag cttgtcttta gagctggcat aatgccgatg
    10801 atgtaggatt tatcaacttt ctgtggctta acgttgatcg ttttcaccac ctctttgctc
    10861 ttaattttaa gggcaagttt ttggtttttt tctaaatgac tagttgactt ttctactgct
    10921 gctgtcaact gttcccagtt agaaactttg tgcgacccta tttgtaaaat acggtcatta
    10981 ttttttaaac ctgcactagc agcaggacca ttttctgata cacggacctg attggttgat
    11041 aaatcttgaa cacctccttg aataaaagca agtgcaataa agactacaag acctaaaata
    11101 aaattattca tcggacctgc aaaattggta attaagcgcc cccatacaga agcattctga
    11161 tactgcacat ctaagggagc aatacgaatt tcagtaccat cctcctcaat gatagtagca
    11221 tcgtgatcaa cactataggt ttttgtctca cttaagacca atcctgtgat agttagttta
    11281 tcttctaaat cataagctgt tacattgatt ggtaaagaag tattgtctaa ttgttttcct
    11341 gataaattaa tacgagttac aatgccttct ttatttaaag taaggctagc cggcgtccct
    11401 gtcttaattt cagtcttatc atcaccccaa ccagccatac ggacataacc acctagcggt
    11461 aatatacgaa tagtataggt tgttccttct ttatcaatgt gagaaaagat ttttggaccc
    11521 ataccaatag caaattcacg aacaagaatt cctgatttct ttgcaaaata gaaatgccca
    11581 aattcgtgaa caactacaat gacaccaaaa ataataataa aggttaaaat tcctagcatt
    11641 caattcctct ctaaatacaa tatattattt tagaataagc caaagaagtg catgattggg
    11701 aaaacaaaaa tcatactatc aaagcgatcc aaaatccccc catgacccgg aatcaattta
    11761 cccgaatctt taacaccaaa atgacgttta atactacttt caactaaatc tccaaattga
    11821 ccaaaaatgc taaagatagc cactaaaact aacatgacta ggaagctatg aggcgcatag
    11881 acggtcttat caaaaagcat aaaaaagaaa gctaccacaa tggctgaagc aattcctccc
    11941 agactccctt caattgtttt atttggagac actgaaggta gtagtttgcg ttgtccaaat
    12001 tgacgaccaa tcatataagc accgatatca gtcgcccaga caataaataa agctaacaac
    12061 accttatcta ttccagccat acgagcactg actagatttt gaaagccaat acccacataa
    12121 aaactagacg caattggaaa ggcagcatct tcaaaactgt aagaattact gttcaaaaca
    12181 gtacctgcca aaatcataaa aataacaata ccgtatgctg ataaactagc gtcaactggt
    12241 aaaaaggaca aataactatc taaaggaact gttaaaacaa aggctcctat catagctaat
    12301 gcaccttcaa aggaaaatat ttctaaacga cgcatacgaa gtaattcaga aacaccaatc
    12361 atagctaaaa gtccaactaa aaattggaaa ggcaaaccac ccattactaa aaaagggata
    12421 aatatggcaa gcgcgacagc tccccaaata acacgttctt tcattttctc tcttctttct
    12481 atacactacc aaatcgacga tggcgttgat tataatccac aatggcctta tgtagttcgt
    12541 cttttttaaa atctggccat aaaacgggag taaagtaaaa ttcactataa gctgattgcc
    12601 aaggtaaaaa gttactcaag cgtaactctc cactagtgcg gataatcaaa tcagggtccc
    12661 tataaagata gggtaagcta tttgtcatta aatgatttgc tactaaatcc tctgtaatat
    12721 cgtcaggatt caacttagct tccaaaacat cctgagcaat ttccttaatg gcattagtga
    12781 tttctgaacg tccgccataa tttagcgcaa aattcaatac gagccccgaa ttatgttttg
    12841 tcctcaaaca agcgcgttgc attgcatcgt atgttgcctt aggtaattta tgagtatctc
    12901 cgatgacctg tacacgtaca ttatttttat ctaactctgg tacatattta tcaaaaaatt
    12961 caaccggaag gttcataata aacttaactt catcatctgg acgtgaccaa ttttctgtag
    13021 aaaaggcata gacagttagt actttaacac ctaattctga agctgctatt gtaacttctt
    13081 gaagagcatc catccccgcc ttatgtccca taacgcgtgg ttttaaacgt ttttttgccc
    13141 aacgtccgtt accatccatg ataataccaa tatgtgctgg tattttctcc aaaggcattt
    13201 ctacttgttt ttttctttta aatataaaca taatgttcct taatttagca atcgtttttc
    13261 ttatcagccc ttttaaaaga gctggaaata tcccatacta taacatttta ccacatttta
    13321 acttattttt gagtattaaa atgccatttg tgacagcaaa ctaaaaagac cagaacaatc
    13381 atctggtcct acatatctta ttcagtttcg atggctgttt ctttattttc gtcttcattg
    13441 acttcaataa tcttaacatc ttcaactggt tctgttggtg ttgctgcctt agagacaaca
    13501 gatttgatag ctgccaactc aaacgtcaag tagacaccat caacatctaa aaccattcgt
    13561 tgtgcttcag tattaacttc atcaactact ccaaaaagac caccaattgt gacaatttca
    13621 tcaccttttt gaacagcatt caattgtttt tgacgttctt gtgcttgttt cttttgctga
    13681 cgttgcatga agaacatcat accaaccata acaacaagca taataatagg taagctcatt
    13741 tgattctcct ttccgtgttc tacgtaatca ttactatagc agatttatgc tatatctgca
    13801 aggagatact gtctcttagc caataaattc acggatttct tcttttgtca tttttgaatc
    13861 gcacttcacc ttgatagttc cgtttttaac aaccatcaaa cctggcactg taggaatgtc
    13921 atatttattt cgaaaatctt tatttgcagt ccaatgatca aaatcttcac tatctaaatg
    13981 gaataattca aattcatctt catcagctac ttctacaatt ttaggaacaa agaggtgaca
    14041 aaatggacaa gaaatacgac caatgaacag tacaaaatct tgatttgaat caattttatc
    14101 ttcaatttcg gcagccgtta tcaaagtcat ggttgccaaa agttcttcaa aagaatacat
    14161 agttaaccaa ctttctaaat cttatattta aagaataacc tatgatggca aaacttgcaa
    14221 ggcaagagta acaacgattc tatcaaaatc attaactcac ttctcgcttc aaactatcct
    14281 caaaaatgct cactcatatt taaatgactc atcaaaaaaa ctgagcatag ctcagttttt
    14341 attttacacc ttatttttgt acagcattac taacgctttt agcaactaca tcggccacat
    14401 cattttgaaa agcatttgga atgatgtgag ttgtgcttaa ttcttcctcc ggtattaaac
    14461 tagctatgcc tctcgctgcc gcaatttgca tttcaacagt tatcgttttt gctctagcat
    14521 ctaatgctcc tctgaaaata cccggaaatg ctaaaacatt attaatttga tttggaaaat
    14581 cactacgacc agttccaact atgtaagctc cggcttttaa ggcttgatct ggataaattt
    14641 ctggaatagg attcgccatt gcaaatacaa taggtttatc agccatcttg gaaatccatt
    14701 ctgcatgtaa tgcttccgga gcagaaacac ctataaaaac atcggcattc tccaaagcat
    14761 cttctaagga tccagattgg aattctctat tagtcaattt tgcaatcgct ttgtgatgag
    14821 gggctaatga ctcccgatct ttatcgttaa taatgccaaa tcgatcaact accgttacat
    14881 gttttgctcc tgctgataga agcttccttg ttatagataa gcccgctgaa cctccaccat
    14941 taacaactac tttgatatct tcaatgtctt ttttaactaa cttcaaactg ttaaacaaag
    15001 ccgctaatac tacgattgct gtaccatgtt ggtcatcatg gaaaactggt atatcacatt
    15061 cttcaattag gcgttgttca atttcgaaac aacgtggtgc tgaaatatcc tctaaattaa
    15121 tccctccaaa agtcggcgaa atcgctttta ctatagaaat aatttcctct gtatcatttg
    15181 ttttcaacac aattggcaca gcatctacat ttgcaaaacg tttaaagagt gctgctttac
    15241 cttccatgac tggcattgct gcctcaggac aaatatctcc taatccaaga acagctgaac
    15301 catcacttac tacggctact gtattttttt tagtcgtcaa ctcatatgca agctccacat
    15361 cctctgcaat agccgatgat actgacgcca cccctggggt ataggcaata ctaagatccc
    15421 tcttatcttc caccttaact tttgaaatta cttctaattt tccaccattt tctctagctt
    15481 ggttgatagc taattgacct aaattttcac tcattttgat aactcctttt gataaatatt
    15541 agaataacat gcgtaaaatg gctgtcatcg tgataacagt aatagctcct cctaaacgcg
    15601 tagcaacttg agcaaatggc atcaagttca ttcggtctgc tgtacttaaa atggcaacat
    15661 ctcctgttcc tcccatacca ctttgacaag ctgaaataat acctgcttca acaggattca
    15721 tattaaggaa tcgcgataca aagaatccaa cagaaataac tgtaaataca acactaataa
    15781 caactatgaa atattgtatg ctaagcgttg ccacaacatc ttttaacggg atataaagca
    15841 atcctagccc tgccattaga ggaaatgtaa aattaccaga aataaattta taaagttgct
    15901 tagctccatt ttgtgtttct ctaggaataa cattgatata tttcaaaata gctgctgcga
    15961 taatcattaa tacgggacca ggaaatccag ttaattgctg caacaaatgt ccaaaaataa
    16021 agagactaca tgctgttaaa acacctccac ccattttctt aacgtcaatt gggccagaat
    16081 gatcagcaat aatatctgac atgtcctctc ctttatttaa ccttactaat tgcccttgac
    16141 cagacaagtg tggtttcttt tctcccaaac gattcaatag tgcagtacat aaaatggcaa
    16201 agaaattacc aataatagtg gctgggatga gttgagcaac tagttgttcg ctagctacac
    16261 cggtaattga actatagcct aacgataatg gtaaaatacc ttcaccaata ccaccagcta
    16321 aaacgggtgt tacgatataa aacaaagtgt gtcgccactc taagcctaaa ataacaccga
    16381 caaatgtccc taccatcatc gcacaaacca ttcctaataa catggggaaa atcattctta
    16441 gtaatccctg aatcaacatt ttccgattca tccctaaaat actaccggac actaaacaag
    16501 caatataaaa ataaagaaag ttagcttgct tcatcaacat atttgttgac tctaaaacat
    16561 ttttattaat gaggttgaaa aacaccaaaa tagatggtac taataaagat aaaatagctg
    16621 ggccaccgaa gtttttaaaa ccagggatgc tagctccaat agtacctaag aaccatccca
    16681 ttgttaagat gactgcaaat cctcctagca tattgactgg caatttttgt aaaaagcctg
    16741 ctagaagaat caataatgct aaacaaacat atactggtaa gggaactgag cctaccttaa
    16801 tatgaaatag tttagtcttt aagtcttgct tttgacctct agaaccctca ttattcacta
    16861 ccttcacgtc tgccatgaaa gcacctcctt ttacttctta tgtacagttt actttgggac
    16921 ttagtgataa ggaagtttat ttaagtaatt gaattatttc aacttattta attaatttaa
    16981 gctggattct agtttttagg ctatgttata ctaaactcgg aggatgctga tgaaaaaaaa
    17041 actatctctt tgggcatttc tatcattgat tttagtcact atgaccattt gtatattttc
    17101 tattttttat tatgtcacta tacatcagtc ctatcgtatg gtaagggtac aggaagaaaa
    17161 aatcttaaaa aatactggtt atgctttatc gagaaatcca caagttatac aaacactcaa
    17221 agataatcac tataatcaaa gtctccaaaa acaaatgcta ttccttagca aaaaatcaaa
    17281 tttagactat attgtcctta taaatttaaa agggatccgt tttacccacc ctgactccac
    17341 taagattggt aaaccatttc aaggcggtga tgaacaagct gtttttaagg gaaaagctat
    17401 tatgtctact gcagaaggtt ctttaggaaa atctttacga tacttaattc cagtatatga
    17461 tcaccaaaaa caggttgggg caattgcagt tggcttaaaa ttaacgacac ttggagactt
    17521 atctcaaagc agcataaaag agtttagtaa gcctctcttg attagtattt tgattagctt
    17581 agtcgtcaca agtataattt cttatggctt aaaaaaacaa cttcacaacc tccatccaag
    17641 tgatattttt caacatttag aagagcgcaa tgcaacactg gaccaaattc aagccgctgt
    17701 atttgttatt gaccagaggc atatcatcaa aagaaataat cctgcagcta gtttattatt
    17761 caaaaaagag gggcaacgag acctattttc aggtaaatta ttagaatctt taattcctca
    17821 actcaagcag gatcattttt caaaaaaaac tgaacaagta ttacattttc aaggacaaga
    17881 ctatttacta tctatctcgc caattacggt taaaacacaa aatagaggct atgttgtctt
    17941 cttaaggaat gttactgaaa cacttttcac tttggatcaa ttagcacaca ctacagctta
    18001 tgcttctgct ctacaggccc aaacacatca atttatgaat caacttcatg ttatttatgg
    18061 acttgcagac atcgaatact acgacgagtt gaaaatttat ttaaaagaat tattggagcc
    18121 acaaaatgaa tttttagcac gcttatcaat gttagttaga gagcctcgac tggctagttt
    18181 cattattggc gaaagagaaa aattcgcaga aaaacacatc aatctatcca cagaaatttt
    18241 agtagaaatc cctaccaagt caactgttga ggatgttaat aactatctct tgctccacag
    18301 atatattaat acgaagatac tcacgctatt aaattcaacg actttagttt ctttacgttt
    18361 aaattatcaa aataacctca ttgaaacaga ttatcaatgg gagaatgaga agtggttact
    18421 caatgactac catcagtatt tcaacgacgc ttattttcaa cagttacttg tagattcaag
    18481 agctacttat aagatctctt taaaacaaaa taaggttatt ataagaacaa atattatcta
    18541 ctcagaaggg tcttagtatg gacgtattaa ttatcgaaga tgatcctatg gtggaattta
    18601 tccaccgcaa ttaccttgaa aaactcaatt attttcaaaa catctattca actgctagtc
    18661 aaacacaagc tattgcttat cttaatgaca ttaaaatcca acttgttctt ttagatattc
    18721 atattaaaga aggcaatggt ctagaattat taaagttatt acgcaatcaa caccaaaata
    18781 ccgaagtcat tgttatttca gcagccaacg aggcgcacac tgtcaaagaa gcatttcatt
    18841 taggtattgt agattacctg ataaaaccat ttacatttga acgctttgag tctagtattg
    18901 aaaaattcct aaatcattat catacatttg aagctgataa aatctatcaa gacaacatcg
    18961 atcactttca aaaaatagat tctggatggc tagagggaga ggttaaactt gatgagaaag
    19021 ggctctctga aattacctat cagcatattc ttgatgccat tcaagagtta gagcagcctt
    19081 tcactatcca agagttagca aaatgcagtc aattttctca tgtctctgtt aggaaatata
    19141 ttgcctatat ggaagaaaaa ggccttctga caagtcagca aatttatact aaggtcggtc
    19201 gtccttacaa agtttataag cttataaaaa aaacttaggc ctgtagacct aagttttttt
    19261 aatcttcgta gccatttgga tgactagaat gccatgccca tgccgatgaa ataattttat
    19321 caatattatc aaatttaggc tcccacccaa gcatttgacg agctttctct gaggaagcaa
    19381 taagcgtatc tggatctcct ggacgacgag ctgccttttg agcaggaatt tctttcccag
    19441 taatacgacg agccgcttct aacatctgaa ggttagaaaa acctgtcgaa gatccgagat
    19501 taaacacgtt tgattcattc ccttggcgaa ggtaatcaac tgctaatata tgagcatctg
    19561 ccaaatcaaa tggatgcaca taatctcgaa cattagttcc atctggagta ttgtaatcat
    19621 ctccaaaaat cattattttg tcacgtactc cttgagcaac ttgaagaatg attggtaaca
    19681 aatgtgtttc tggtttatga tcttccccaa ttgagccatc cggtttatct ccagcaacgt
    19741 tgaagtaacg tagagcgaca aacttaatac cgtaggcttg atcagcccat ttcataattg
    19801 tttccatcat aagcttactt tcgccataag gattaatagg attttgagga gctgtttcta
    19861 aaataggaac ttgttctgga ataccatatg ttgcagctgt tgatgaaaag acaatatttt
    19921 tgatatcaca ttcattcatt acttctaaaa gttttatcat tccagcagtg ttattatcaa
    19981 aatattttaa aggattttcc attgactcag caactagtga aaatgccgca aaatggataa
    20041 cagcatctac atcaggattt tccctaaaaa cttgacgcat aaacgtttta tcggataaat
    20101 ctccttcata aaaaatcgca tctgaatgca ccgcttgacg atgacctgtt accaagttat
    20161 caacaacaat aactttctct tttccttgtg tgattaactg atcaaccata tgcgagccaa
    20221 tgtaaccggc gccacctaaa atcagaactg ccatattttt ctcctatttc aatactcttt
    20281 taacatcatt tgttacctca ttatactgag aataagcttt ttttgcaaat atctaccgaa
    20341 attagaatag ctctatgcag aaggcatccc acggcttgag agtgttactt gatagtactt
    20401 cttgaacctc tgtatttgaa ataatagttt ctttaacagc ttctggaaat tcaaaagatt
    20461 ggttttgatc ggataagttt gcaactatta aataacgttc tttatctgtt tgacgaacat
    20521 aagcaaatac cttatctgct gtttccaaaa gtttaaaatc agcatcaact aaccagtcgt
    20581 gttctttacg taaagcaact aatttttgat aagtataaaa aatactttcc gtatcattta
    20641 acgctgcctc cacattaatt tcctgataat tgggattgac tgctagccat ggggtaccgt
    20701 tagtaaaacc agcttgatgt ccttgactcc actgcattgg agtacgagca ttatcacgac
    20761 cgactttacg aatactatct aaaattgttg cttcagatac cccattatcc atggcttctt
    20821 tagcatagtt aagggattca atatcatcta catctgctaa acactcaaat ggataattgg
    20881 tcataccaat ttcttcacct tgatagatgt aaggcgttcc acgcatcaaa tgtagtagga
    20941 ttgctagagc cttggcagat tgttttctgt tatcactatc atttccccaa atcgagagca
    21001 cccgaggaag gtcgtgatta ttccagaata aagaattcca accttggcca agctccaatt
    21061 gtgtttgcca ttttgtaaaa atatctttca atgctggaac atctaatccg tcactgtaat
    21121 cccacttagg agcgtcaggt ttatgttgaa gacctacatg ttcaaattga aagaccatag
    21181 ataattcttc attatcggga tttgagtatt gttttgcaat gtctggcgtt gctccccaag
    21241 tttcacccac cgtcaataag tcatgctttc caaaactagc tctgttcatt tctttaagat
    21301 aatcatggag ttttggtcca ttaccagtaa tgcccttatc tggaattttc ccaattaaat
    21361 caatgacatc catacggaaa ccaccaatac ccttatcaat ccagaaattc atcatatcat
    21421 aaattttatg acgtaaagct tcattctccc agttgagatc tggctgacgt ttgctaaaaa
    21481 ggtgcaaata atattgacct gatgctttat catattccca agctgagcca ctaaaaatag
    21541 aagtcaaatc gttaggttca tctcgccaaa tataaaaatc acgttcagga ctattagggt
    21601 tttctcgcgc ttctacgaac caagcatgtt catcagaggt atgattcact accaaatcca
    21661 tgatgatttt aatcccgcgt tgatttgcct cttgtaacag ttggtccata tcattcatgt
    21721 caccgaaaat atctgcaatg gcttgataat ctgaaatatc ataaccattg tcatccattg
    21781 gactttggta aacaggtgat aaccagatag cagtaatccc taatttttca aggtagtcta
    21841 gtttgctagt aatcccttta atgtccccca caccatcacc atctgaatcc ataaatgaac
    21901 gtgggtaaat ttgataaatc gtagctttat gccaccagtg tttcttcatt attcttctcc
    21961 tatcaataat tcattcatct tttattttaa cgtttttcct cttttagtgc aagcgtttgc
    22021 gcaaataaca ttataaaaag gctggaaccc aaccttttta tgaatatcgc ttatacctta
    22081 acgaatagct tgttcagtat ctgcatcaaa gaaatgcccc ttagcaacat taaacgttag
    22141 gttaactttt tctcctggat tatggaagtc acgcgcttca acacgagaag caaattctgt
    22201 ttgacctaac ttaacataaa gcatagtttc gctaccgagt aattcagaaa ctaaaacttc
    22261 tgcttcaaca ttggcatttg gataagtatc ttggacaaga agattgcttg aaatgtcttc
    22321 tggacgaata ccaaaaatca attcttttcc tttatagcct cgtgattcta aaagtttttc
    22381 ttgaccttca gggatagcga ttcgaagacc atcttcacta ataatcatac cattttcaac
    22441 tttaactttg aagaagttca tagatggact accgatgaat cccgcaacaa atttattagc
    22501 aggaagatta tagagttctt gtggtgaacc aacttgctca attttaccaa ttgtaccatc
    22561 accatcaggg ttcttagtag ctgacataat gacaatacgg tctgccaaag tcatcgcctc
    22621 tgtttggtcg tgggtaacat aaatagttgt tgaaccaata cgttgatgaa tttttgcaat
    22681 ttcagcacgc attgaaacac gtaatttagc atctaagtta gataaaggct catccattaa
    22741 gaacactttg gcatcacgca caatagcacg tcccatagca acacgttggc gttgtccacc
    22801 tgacaaatcc gcaggtttgc gctctaagaa ttcagtaagg ccaatattag ctgctgcttc
    22861 acgaacgcgc ttatcaattt cttgttttga gaatttacgc aatttgagcc caaaagccat
    22921 attatcgtaa acagtcatat gtggataaag agcatagttt tggaaaacca tagcaatatc
    22981 acgatcttta ggtgatttgt cattgacaac ttcgccatca atcttgagct caccttcact
    23041 aatatcttca agacctgcga tcatacgaag tgttgttgat ttcccacaac ctgatggtcc
    23101 aacaaaaaca ataaattctt tgtctttaat atccaagtca aagtcttcaa cagaataatg
    23161 cgatgcactt ggatattttt tgtaaatatg atttaaattt aattctacca ttagattgac
    23221 ctcttttatt attgtgattt tattatagca aagaaagggt tttcatataa tggtaacctt
    23281 gcctaaattt cttgtgcaaa ctaactaaat tttaggtatc catcgaaatt aaataggcat
    23341 aggctaaatc atctaatatt tttaaattaa gccccgaaga ttgggtaaat ttatctaatt
    23401 tatattgcaa tgaattccga tgaatgaaaa gtgccttagc cgttttagca aggttccctt
    23461 gctcttgcca caaggctttt attatagccg aagtatcact cgtatcaagg atatgttgta
    23521 aacattttgc tttaatagaa ggcatgggcg attgatttgc caaagcccat aaagccatct
    23581 ttggaaatcg acagtaatgt tgttcggcct tgtgactaag aaaaagcgta aaaagtttat
    23641 attcttcttc atataattct ttccagtcca ctgcttgtag atgcgtccaa ctatttccaa
    23701 aatagcatct taaacgcgtg ttaaagtcat tttcaatcgt tggtaaaata tctgttagta
    23761 attccgtagc attaaagaaa ttatcctgat ttaaaatcat gacactttgg gtttgattaa
    23821 ttgtcataat tgtttctacg tttattacta aaggcgacaa gaaatcctta agttcttctg
    23881 ataaggtaaa ttgatgctca ataaaaataa attggactgc cgaataagat tggggcaacc
    23941 ttccacgacc ttcaactaga tagcggtacc aaggattctg agaatccact gaactcgcct
    24001 cttccaggct ttgtgtcatt tgtaataagc atagttcacg cttagttagc gaagattttg
    24061 gaaagtggag gacatgacca tcttgctcaa cgatgatata atcctcactc tgtttaactt
    24121 cctgtttagt aataacttct ggaaacagtt ccttaaaatc catggcatcc tccactacag
    24181 ttttgttcat tatatcatat ttataacctc tttttgagca cagaaaaaga ggagaatgtc
    24241 tcctcagaaa gtttctgtta taaaatagtg atccgaatag aatgctgttt cgactgcttt
    24301 ggattaagcc gtacgatatt ttgtttatct tctaaaatat cagactcttc aatagaagtt
    24361 gacaaggaac tccagggttc caaagccaaa aaaggaccac cattgttact tgtccaaaga
    24421 ataagattct caaaatcatc aaagtcaagt tggattcctt ttgcatgatt tctagatttt
    24481 aagtagaccg tcttagatcg tagttggtct aaagtaatag catctttttc aaataaagca
    24541 taattaagag ataattgttt ttgattttct aaaaaaggat gtctagcttg taaatctaac
    24601 aagcctgtat ccggaaacga ctcaggaatt gtgcaagttt caaactgttc aaactctaga
    24661 tagtaatcac taaatacttc cttttcaaac aaagggcaat taaagcctgg gtgagctccg
    24721 atagcatatg gcatattttt ttctgattct aagtttttga caatatatgt aatggcaatg
    24781 cttttatttc ttaaggtgta agttatttct aaactaaaac gataaggata atttatcaac
    24841 gtttcttcac tacttttaat tgtaaaacga acagaagaat ccgatatgta atcatagtcg
    24901 aatgttttgt gacgtacaaa gccatgcctt ggtatgatac ccgtaaaatg tggagcttga
    24961 gctggtctgt aaatgacgca atcatttcga acacttccgc atataggaaa taaaatagga
    25021 gcttgcccac cccaataagt gggttcccct tgccaaaggt actcaacgcc ttgactatct
    25081 gtaatagaag tcattgttgc acctaacgct tttaatgtca cttgcaactc atgattttgt
    25141 atcgtaatag ccattatttt tcctttcata aagagcaata attgctttac caacttttct
    25201 ctagcaatta tctttccttc caagaagttg ctgttttttg caaaatttta tttagttcat
    25261 caatattttg aaaacctatt gtacgtaacc attgacgtgc accagcttct cctttttcaa
    25321 catactcttt aacacttcca gcccaagttg ctctaccaca aaggacaccg ttaaatttag
    25381 caccagcttc tttagcaaac acaagagtct cttgaaaaag ctgagcagga actcctgcac
    25441 tcaaaaaaat gtaggggagt agtgtcgcct gatcttgttc tctaaaataa gccgcagctg
    25501 tcgctttatt ataagcagtc tctccttgcg caaaaccttc tacatagtcc atattgacag
    25561 gcacttccac cttcaagaca tcaatattaa atcgaggatt tgaaaaaact ttcatagctt
    25621 caataacctt acgtggctta attttggcat actcaatacc tgagctatcc gtaatctttt
    25681 catcatatga caaaatttca aggaaaaaag gaatgtcttc tgcaacacat tcagaaccaa
    25741 tccgttcaat ataagcttct ttttcttcgt ttacttcatc cgaactatca acatcataat
    25801 aaagtaagaa cttaactgcg tctgccccct cttctttaat gcgtttcgca gaccaaatgt
    25861 ctaaacagtc tgggagtcgc ttggtactac tggtatcata acctgttttt tcgtacgcaa
    25921 gtaaaagacc ggcattgtca tctaaaactt tcgtagctgg caacccatat tctggatcta
    25981 acaacattga tgaagcgtaa ggggttaatt cttcagctac caaaacttta agtgcttcta
    26041 tttgagaaac agtgggctcc tcagattgat attttgccat taatcgcttc aatgctccac
    26101 gttggtcaaa agccaaggcc gaaataatac catttttatc actcaattgt tccatatgtt
    26161 tttgtttttg ttccgttaat cccatcctat acctctttca cttggatttg actgattaat
    26221 tcttgatagt aggtcagatt gatatgccct gttaatgttt cttgtgcatt aagcataccc
    26281 aagacattag ctctcttaag taaatttata tcatctagtt gatgttctaa agcagacgcg
    26341 attcctgcaa ccgttgcatc ccctgaacca actggattga taacttctat ttttggaatc
    26401 gtcacttgat agtagtgatt cccatgttta gcaaaagctc ccctagcacc taaggaaaca
    26461 ataacccaat caattcctct aaaaatatct tgctgtaagg tagattttaa ttcgtctaaa
    26521 ctatagctaa ttggccttcc aattaattgt gttaattctt caaggttagg tttaatgacg
    26581 gtcggcttag cactagattt taaaaccgaa cgtaatgctt ctcctgaaca gtctaaaaca
    26641 actttcttat taaagtgatt cgctagggag attaacctag cataataatc tgaggggagt
    26701 ccctttggta aacttcctga tactgttatg atagttgcag catcaaagat aagttttaga
    26761 tgattcaaaa aggcgcctgc ttcatcttta gtaatatgtg gacctttttc tagaatttca
    26821 gtctgctgac cctcatgtaa aactgcaata caatgacgtg tttctccacc aatacttaaa
    26881 aattggtgtc tgatttccaa tgcatctaag ccacttcgga tgaaatctcc aaaatgcccc
    26941 cctaatagac cagttgccac tacattatcg ccaagttgag aaagaacacg agtcacattt
    27001 aatcccttac caccaggtgt tttggagacg tcggtaaccc
//
LOCUS       DATROQ010000016        26059 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.016, whole genome shotgun sequence.
ACCESSION   DATROQ010000016 DATROQ010000000
VERSION     DATROQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26059
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.016"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            416..1582
                     /locus_tag="VBC94_000648"
     CDS             416..1582
                     /locus_tag="VBC94_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6978941.1"
                     /translation="MKITEVKKKDGTVIYRASIYLGTDKVTGKKVTTKITGRTKKEVR
                     EKAKQEAVEFIKNGSTRFKATSVTSYQELATLWWDSYKHTVKYNTQLATEKLLTVHVI
                     PIFGAYKLDKLTTPLIQSIINKLADKTNKGERKAYLHYDRIHALNKRILQYGVIMQAI
                     PFNPAREVILPRNTKKANTKRVKHFENDELRTFFNYLNNLDKSKYRYFYEVTLYKFLL
                     ATGCRINEALALNWSDIDLDNAVVHVTKTLNYKQEINSPKSKSSYREIDIDNQTVTML
                     KQYRRRQIQEAWKLGRSETVVFSDFIHKYPNNRTLQTRLRTHFKRANVSNIGFHGFRH
                     THASLLLNTGIPYKELQYRLGHSTLSMTMDIYSHLSKENAKKAVSFFETAINSI"
     gene            complement(1689..2300)
                     /gene="rpsD"
                     /locus_tag="VBC94_000649"
     CDS             complement(1689..2300)
                     /gene="rpsD"
                     /locus_tag="VBC94_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEO6978942.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKAKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(2630..2917)
                     /locus_tag="VBC94_000650"
     CDS             complement(2630..2917)
                     /locus_tag="VBC94_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEO6978943.1"
                     /translation="MSDAFADVAKMKKIKEDIKSHEGQMVELTLENGRKREKNKIGRL
                     IEVYPSLFIVEYKDTAAVPGAIDNTYVESYTYSDILTEKTLIRYFDDESAE"
     gene            complement(2929..4284)
                     /gene="dnaB"
                     /locus_tag="VBC94_000651"
     CDS             complement(2929..4284)
                     /gene="dnaB"
                     /locus_tag="VBC94_000651"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO6978944.1"
                     /translation="MVEVSELRVQPQDLLAEQAVLGSIFISPEKLIMVREFISPDDFY
                     KYSHKVIFRAMITLADRNDAIDAATVRNILDDQGDLQNIGGLGYIVELVNSVPTSANA
                     EFYAKIVSEKAMLRDIISKLTDTVNMAYEGNDSDEIIATAEKALVDINEHSNRSGFRK
                     ISDVLKVNYENLELRSQQTSDVTGLPTGFRDLDRITTGLHPDQLIILAARPAVGKTAF
                     VLNIAQNVGTKQNRPVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNVT
                     IAQGALADAPIYIDDTPGIKITEIRARSRKLSQEVDDGLGLIVIDYLQLISGTRPENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYRREGEEAEEIVEDNTVEVILEKNRAGARGTVKLMFQKEYNKFSSIAQFE
                     E"
     gene            complement(4327..4779)
                     /gene="rplI"
                     /locus_tag="VBC94_000652"
     CDS             complement(4327..4779)
                     /gene="rplI"
                     /locus_tag="VBC94_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEO6978945.1"
                     /translation="MKVIFLQDVKGKGKKGEVKEVPTGYAQNFLLKKNLAKEATTQAI
                     GELKGKQKSEEKAQAEILAQAKELKTQLESETTRVQFIEKVGPDGRTFGSITAKKIAE
                     ELQKQYGIKIDKRHIDLDHTIRAIGKVEVPVKLHKQVSSQIKLDIKEA"
     gene            complement(4785..6767)
                     /locus_tag="VBC94_000653"
     CDS             complement(4785..6767)
                     /locus_tag="VBC94_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEO6978946.1"
                     /translation="MKRFRFATVHLVLIGLILFGLLAICVRLFQSYTALLLAIFVALS
                     FVVALLYYQKITYELSEVEQIELLNDQTEVSLKSLLEQMPVGVIQFDLETNDIEWFNP
                     YAELIFTGDNGHFQSATVKDIITSRRNGTAGQSFEYGDNKYSAYLDTETGVFYFFDNF
                     MGNRRNYDSSMLRPVIGIISIDNYDDIMDTMLEADMSKINAFVTSFISDFTQSKNIFY
                     RRVNMDRYYIFTDYSVLNTLIKDKFDILNEFRKRAQENHLSLTLSMGISYGDGNHNQI
                     GQIALENLNTALVRGGDQIVVRENDSSKKALYFGGGAVSTIKRSRTRTRAMMTAISDR
                     LKVVDSVFIVGHRKLDMDALGASVGMQFFASNIVNASYVVYDPNDMNSDVERAIDYLQ
                     EDGETRLVSVERAFELITQNSLLVMVDHSKTALTLSKEFFNKFADVIVVDHHRRDEDF
                     PKNAVLSFIESGASSASELVTELIQFQQAKDKLSRSQASILMAGIMLDTRNFASNVTS
                     RTFDVASYLRGLGSNSMAIQKISATDFDEYRLINELILKGERIYDNIIVATGEEHKVY
                     SHVIASKAADTMLTMAGIEATFVITKNSSNIGISARSRNNINVQRIMEKLGGGGHFSF
                     AACQIQDKSVKQVRRMLLEIIDEDLRENSTVENRRD"
     gene            complement(6834..8735)
                     /gene="mnmG"
                     /locus_tag="VBC94_000654"
     CDS             complement(6834..8735)
                     /gene="mnmG"
                     /locus_tag="VBC94_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEO6978947.1"
                     /translation="MTHNFAENYDIIVVGAGHAGVEASLAASRMGCKTLLATINLEML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KALYAQTMKQTVEKQENLTLRQAMIDEILVEDGKVVGVRTATNQKFSAKSVVITTGTA
                     LRGEIILGDLKYSSGPNNSLASVTLADNLRDLGLEIGRFKTGTPPRVKASSINYEKTE
                     IQPGDEQPNHFSFMSRDEDYITDQVPCWLTYTNTLSHDIINQNLHRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRYTEEVYVQGLSTSLPEDVQVDLLRSIKGL
                     ENAEMMRTGYAIEYDIVLPHQLRATLETKVIAGLFTAGQTNGTSGYEEAAGQGLVAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGYEIGLVDEERYAIFKKRQMQFENELERLDSIKLKPVSETNKRIQELGFKPLTD
                     ALTAKEFMRRPQITYAVATDFVGCADEPLDSKVIELLETEIKYEGYIKKALDQVAKMK
                     RMEEKRIPPHIDWDDIDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEG
                     RQKGRKNIN"
     gene            complement(8905..9516)
                     /locus_tag="VBC94_000655"
     CDS             complement(8905..9516)
                     /locus_tag="VBC94_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEO6978948.1"
                     /translation="MISKFILAFMAFFAIMNPISNLPAFMALVADDDQKISRRIAAKG
                     VLLAFVIIVIFVLSGHLLFNLFGITLAALKISGGILVGIIGYKMINGIHSPTNKNLEE
                     HKDDPMNVAVSPLAMPLLAGPGTIATAMGLSSGGLSGKLITILAFAILCVIMYVILIS
                     ANEITKFLGKNAMTIITKMMGLILMTIGIEMLITGIKIGFHLT"
     gene            complement(9548..10669)
                     /gene="mnmA"
                     /locus_tag="VBC94_000656"
     CDS             complement(9548..10669)
                     /gene="mnmA"
                     /locus_tag="VBC94_000656"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEO6978949.1"
                     /translation="MTDNSNIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVTRDENGIVHMLRGADNNKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKPEVRRIAEEAGLATAKKKDSTGICFIGEKNFKDFLGQYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNKPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLTASEIHFTRDMPNEFKLECTAKFRYRQPDSKVTVYVKGNQARVVFD
                     DLQRAITPGQAVVFYNEQECLGGGMIDQAYRDDKICQYI"
     gene            10915..11583
                     /gene="sdaAB"
                     /locus_tag="VBC94_000657"
     CDS             10915..11583
                     /gene="sdaAB"
                     /locus_tag="VBC94_000657"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEO6978950.1"
                     /translation="MKHLKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGEPSE
                     VTFHLYNSFAKTYQGHGTDKALVAGILGMDTDNPDIKNSLEIAHQKGIKIYWDILKDS
                     NSPHPNTAKITVKNGDRSMSITGVSIGGGNIQVTELNGFSVSLTMNTPTLIIVHQDIP
                     GMIAKVTDILSDFNINIAQMNVTRESAGEKAIMIIEVDSRDCQQAVKKIEAIPHLHNV
                     NFFD"
     gene            11598..12470
                     /gene="sdaAA"
                     /locus_tag="VBC94_000658"
     CDS             11598..12470
                     /gene="sdaAA"
                     /locus_tag="VBC94_000658"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEO6978951.1"
                     /translation="MFYTIEELVEQANSQHKGNIAELMIQTEIEMTGRSREEIRYIMS
                     RNLEVMKASVIDGLTPSKSISGLTGGDAVKMDQYLQSGKTISDTTILAAVRNAMAVNE
                     LNAKMGLVCATPTAGSAGCLPAVISTAIEKLNLTEEEQLNFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSASAMAAAALVMAAGGTPFQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNALGSSFALVAADMALAGIESQIPVDEVIDAMYQVGSSLPTAFRETAEGGLAA
                     TPTGRRYSKEIFGE"
     gene            complement(12605..13309)
                     /locus_tag="VBC94_000659"
     CDS             complement(12605..13309)
                     /locus_tag="VBC94_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEO6978952.1"
                     /translation="MNKRRKLSKLNVKKQHLAYGAITLVALFSCILAVTVIFKSSQVT
                     TESLSKADKVRVAKKSKMTKATSKSKVEDVKQAPKPSQASNEAPKSSSQSTEANSQQQ
                     VTASEEAAVEQAVVTENTPATSQAQQTYAVTETTYKPAQHQTSGQVLSNGNTAGAVGS
                     AAAAQMAAATGVPQSTWEHIIARESNGNPNVANASGASGLFQTMPGWGSTATVQDQVN
                     SAIKAYRAQGLSAWGY"
     gene            complement(13415..13954)
                     /locus_tag="VBC94_000660"
                     /pseudo
     CDS             complement(13415..13954)
                     /locus_tag="VBC94_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            complement(14170..14964)
                     /locus_tag="VBC94_000661"
     CDS             complement(14170..14964)
                     /locus_tag="VBC94_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO6978953.1"
                     /translation="MDKLILGRYIPGNSLIHKLDPRSKLLAMLLFIIIVFWANNVVTN
                     VIVFIFTLVIVGLSQIKFSYFFNGIKPMVGIILFTTLFQMLFAQGGQVIFSFWIFSIT
                     SLGLQQAALIFMRFVLIIFFSTLLTLTTTPLSLADAVESLLKPLEVLRVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIHKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGANRSKYRLLKWTVRDTFSILLMLLLGLSLFLLKN"
     gene            complement(14957..15799)
                     /locus_tag="VBC94_000662"
     CDS             complement(14957..15799)
                     /locus_tag="VBC94_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO6978954.1"
                     /translation="MGIEFKNVSYTYQAGTPFEGRALFDVNLKIEDVSYTAFIGHTGS
                     GKSTIMQLLNGLHIPTKGEVIVDDFSIKAGDKNKEIKFIRQKVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGISQIEAERLAEEKLRLVGISEDLFDKNPFELSGGQMRRVAIAGIL
                     AMEPKVLVLDEPTAGLDPKGRKELMTLFKNLHKKGMTIVLVTHLMDDVADYADYVYVL
                     EAGKVTLSGQPKQIFQEVELLESKQLGVPKITKFAQRLSHKGLNLPSLPITINEFVEA
                     IKHG"
     gene            complement(15775..16614)
                     /locus_tag="VBC94_000663"
     CDS             complement(15775..16614)
                     /locus_tag="VBC94_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO6978955.1"
                     /translation="MTNIITVNNLFFKYDSNQTHYQLENVSFHVKQGEWLSIIGHNGS
                     GKSTTVRLIDGLLEAESGQIIIDGQELTEDNVWELRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIPLKDMKERVDQALDLVGMSEFKMREPARLSGGQKQRVAIAGAVAMRPQ
                     VIILDEATSMLDPEGRLELIRTIRAIRQKYNLTVISITHDLDEVALSDRVIVMKNGKV
                     ESTSTPKALFGRGNRLISLGLDVPFTSRLMAELAANGLDIGTEYLTEKELEEQLWELN
                     LKM"
     gene            complement(16614..17156)
                     /gene="pgsA"
                     /locus_tag="VBC94_000664"
     CDS             complement(16614..17156)
                     /gene="pgsA"
                     /locus_tag="VBC94_000664"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEO6978956.1"
                     /translation="MVKKENIPNLLTVVRILMIPLFIVLTSVTTSTTWHIVAAIVFAI
                     ASLTDYLDGYLARKWQVVTNFGKFADPLADKMLVMSAFIMLVGLDLAPAWVSAIIICR
                     ELAVTGLRLLLVETGGTVLAAAMPGKIKTATQMFAVIFLLVHWMTLGNIMLYIALFFT
                     LYSGYDYFKGAGFLFKDTFK"
     gene            complement(17279..18562)
                     /locus_tag="VBC94_000665"
     CDS             complement(17279..18562)
                     /locus_tag="VBC94_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO6978957.1"
                     /translation="MTVNKITYQNLQEEVYKLTLESGLNVYLIPKPSFKETVGVLTAN
                     FGSLHTRYTRNGCVEHYPAGIAHFLEHKLFELDKGQDAATQFTKYGAESNAFTTFDKT
                     SFYFSTISHITNCLDILLDFVLTTNFTEESITKEKDIIKQEIEMYQDDPEYRLYQGVL
                     SNLYPNSPLAFDIAGDYQSISQITLTDLQENHKDFYQLSNMNLVLVGQFSPQEIITYL
                     QKNSHLTNYSQNIDRDSISLEPVIKNNSCHMTVTKPKLAIGYRKSSHMRHGSYLKEKI
                     GLQLFFAMLLGWTSTINQDWYESGQIDDSFDIEIEVHPDFECVIISLDTTEPIAFSTQ
                     LRLLLKNALQSSDLTESHLQNVKRELYGDFLRSLDSIENLAMQFVTYLYDGKTMYLDL
                     PSIVEELDLEDVITIGKDFLDNADTSDFVIFPKSS"
     gene            complement(18564..19808)
                     /locus_tag="VBC94_000666"
     CDS             complement(18564..19808)
                     /locus_tag="VBC94_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO6978958.1"
                     /translation="MKIVEGVSLHLIKNQQFKTNHLTFRFSGDFNNKTVARRSLVAQM
                     LVTANAKYPKVQEFREKLASLYGASLSTKISTKGLVHIVDIDIVFVKNTFTLEQENIV
                     EQIITFLEDMLFSPLISLEQYQTSIFDTEKKNLIQYLEADIEDNFYSSDLALKSLFYN
                     NKTLRLPKYGTASLVESENSFTAYQEFQKMLKEDQLDIFVVGDFDDYRMIQAFNRMAF
                     EPRHKVLAFDYTQTYENITRSQVEDKDVNQSIMQLAYHLPITYKDEDYFALIVFNGLF
                     GAFAHSLLFTEIREKQGLAYTIGSQFDSFTGLFTIYAGIDKENRERFLKLINKQFNNI
                     KMGRFSSTLLKQTKDILKMNYVLASDNPKVIVDHIYHEHYLDQFHTSALFIDKVDDVT
                     KSDIVSVATKLKLQAFYFLEGN"
     gene            20047..20400
                     /gene="yaaA"
                     /locus_tag="VBC94_000667"
     CDS             20047..20400
                     /gene="yaaA"
                     /locus_tag="VBC94_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEO6978959.1"
                     /translation="MDYKLFDEYITLQSLLKEIGIIQSGGAIKKFLADNQVLFNGDLE
                     NRRGKKLRLGDIITIPDQNIEIIIRKPSDQEIEERNVEIAEKQRVSAIVKEMNKNTNK
                     DKSKTSKKPVRFPGT"
     gene            20403..21512
                     /gene="recF"
                     /locus_tag="VBC94_000668"
     CDS             20403..21512
                     /gene="recF"
                     /locus_tag="VBC94_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEO6978960.1"
                     /translation="MWIKNISLKHYRNYEEAQVDFSPNLNIFIGRNAQGKTNFLEAIY
                     FLALTRSHRTRSDKELVHFKHHDVQITGEVIRKSGHLNLDIQLSEKGRITKVNHLKQA
                     KLSDYIGAMTVVLFAPEDLQLVKGAPSLRRKFLDIDIGQIKSTYLAELSNYNHVLKQR
                     NTYLKTTNNVDKTFLTVLDEQLADYGSRVIEHRFDFIQALNDEADKHHYIISTELEHL
                     SIHYKSSIEFTDKSSIREHFLNQLSKSHSRDIFKKNTSIGPHRDDITFFINDINATFG
                     SQGQQRSLILSLKLAEIELIKTVTNDYPILLLDDVMSELDNHRQLKLLEGIKENVQTF
                     ITTTSLEHLSALPDQLKIFNVSDGTISINEKKATD"
     gene            complement(21524..22390)
                     /locus_tag="VBC94_000669"
     CDS             complement(21524..22390)
                     /locus_tag="VBC94_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEO6978961.1"
                     /translation="MEGLLIALIPMFAWGSIGFVSNKIGGRPNQQTFGMTLGALLFAI
                     IVWLFKQPEMTASLWIFGILGGILWSVGQNGQFQAMKYMGVSVANPLSSGAQLVGGSL
                     VGALVFHEWTKPIQFILGLTALTLLVIGFYFSSKRDVSEQALATHQEFSKGFATIAYS
                     TVGYISYAVLFNNIMKFDAMAVILPMAVGMCLGAICFMKFRVNFEAVVVKNMITGLMW
                     GVGNVFMLLAAAKAGLAIAFSFSQLGVIISIIGGILFLGETKTKKEQKWVVMGILCFV
                     MGAILLGIVKSY"
     gene            complement(22446..23108)
                     /locus_tag="VBC94_000670"
     CDS             complement(22446..23108)
                     /locus_tag="VBC94_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6978962.1"
                     /translation="MKNYGKILKELREDKGISLSSLAKSAQLSKSTLSRFENGETQIG
                     IDKFIKALQTLEVGVTINEVSILDSKVKAGTSNTDLEQLTLLESYRDNEDIMRIFSFQ
                     EQQSCDRIESNVLKILAKLFISNLGLNMRLPQDEINLVVTYLNGVTQYNDFYFKVICY
                     FQDILPEDVILNKISNMTKEQLPYSKSLVNLLIKQVIIALEKDSVDKAIVFADLANKV
                     SQ"
     gene            complement(23184..24665)
                     /gene="guaB"
                     /locus_tag="VBC94_000671"
     CDS             complement(23184..24665)
                     /gene="guaB"
                     /locus_tag="VBC94_000671"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEO6978963.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNEVDMNTKLADNLTL
                     NIPIITAAMDTVTDSKMAIAIARAGGLGIIHKNMSIVDQAEEVRKVKRSENGVIIDPF
                     FLTPDNTVSEAEELMQNYRISGVPIVETLENRKLVGIITNRDMRFISDYKQLISEHMT
                     SQNLVTAPIGTDLETAERILHEHRIEKLPLVDDEGRLSGLITIKDIEKVIEFPKAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQITAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIVFQMLGGIRSGMGY
                     VGAANIKELHDNAQFVEMSGAGLKESHPHDVQITNEAPNYSVH"
     gene            complement(24822..25307)
                     /locus_tag="VBC94_000672"
     CDS             complement(24822..25307)
                     /locus_tag="VBC94_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO6978964.1"
                     /translation="MNKKETRHQLIRSLVSETKVRTQHELRELLEKNGVSVTQATLSR
                     DMKELNLIKVNESSDNATETYYEIHSISQKRWEERLRFYMEDALVMLFPVQNQIVLKT
                     LPGLAQSFGSILDSILLPEILATVCGDDTCLIICNNSEEAQKCFEKLSQYTPPFFFSK
                     K"
     gene            complement(25317..25997)
                     /locus_tag="VBC94_000673"
     CDS             complement(25317..25997)
                     /locus_tag="VBC94_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO6978965.1"
                     /translation="MITKEQYFYFRQLDDFKHFTIEQFDHIVSHIKHRTALKNHTLFF
                     EGDYREKLFLIQSGHVKIEQSDASGSFIYTDYVRQDTVFPYGGLFLDDDYHFSAVAIT
                     DIEYFSLPMALYEEYSLQNINQMKHLCRKYSKLLRVHEIRLRNMVTSSASMRVIQSLA
                     TLLLQVPTERGHLPFPITTIEIANMSGTTRETVSHVLKELRQKDIVEMKGKKLLYNNK
                     NYFKKFIE"
BASE COUNT         8561 a         5037 c         4062 g         8399 t
ORIGIN      
        1 aaattcgttg attttattat ctctgaaata gaaaaagacc ctcaacattt tgttgaaagt
       61 ctgaaaaaat ctaaataaaa aagataaccc ctggcacata ggaatgctca gcccgaaggc
      121 ttaccccatt ctgggaccca gcgttaatcc ttcacaagtt atctttgtta actatattat
      181 atcacgcacg ttctaaatgt aaaggatttt gttagctatc cagtaagcta actttgtcaa
      241 aaacccctga aaaacagctt taaatcatcc ataatcgcat tttaaccttt aaccaggtaa
      301 ttttaccgac ttctccaaaa caaacgaaat aagaatcttc tcgtaagctc tggcatgata
      361 taaacctaaa atccctttaa taatagcttg cctgctgatg gaaaggttta tgatcatgaa
      421 aataactgaa gtaaaaaaga aagatggtac ggtaatttat cgtgccagta tttatcttgg
      481 tactgataaa gtaacaggta aaaaagtaac tactaaaata acaggacgaa ctaagaaaga
      541 agttagagaa aaagctaagc aagaagctgt cgagtttata aaaaatggtt ctactcgctt
      601 caaagctact tccgtaacaa gttatcagga acttgcaacc ttatggtggg atagttacaa
      661 acataccgta aaatacaata ctcagctagc tactgaaaag ctgttaaccg ttcatgtcat
      721 accaattttt ggagcatata agcttgataa gttaacgaca ccacttatac agtctatcat
      781 caataaacta gctgataaaa ctaataaagg agaaagaaaa gcttacctcc attatgacag
      841 aatacacgcg ctgaataaac gtatactaca gtatggcgtt atcatgcaag ctataccatt
      901 caaccctgcg cgtgaggtca ttctccctcg caacactaag aaagctaaca ctaaaagagt
      961 aaagcatttc gaaaatgatg aactaagaac atttttcaac tacttaaaca atctagataa
     1021 aagtaaatac agatacttct atgaagtcac actttataag tttttattag ctacaggttg
     1081 tcgcattaat gaagcgttag ctctaaactg gtcagatatc gacttggata atgccgttgt
     1141 tcatgtcaca aaaacgctaa attacaagca agaaattaat agtccaaagt caaagtcaag
     1201 ctatcgtgaa attgacatag ataatcaaac agttacaatg cttaagcagt atagacgacg
     1261 acagattcaa gaagcatgga agttagggcg ttcagaaaca gtggtatttt ctgattttat
     1321 ccataagtac ccaaacaata gaaccttaca aactcgatta agaacacatt ttaaaagagc
     1381 aaatgtatcg aatataggct tccatggttt tcgtcacact cacgctagtt tattgctgaa
     1441 cacaggtatc ccctacaaag aactccaata tagattaggg cactctactc tatctatgac
     1501 tatggatatt tatagccatt tatcaaaaga gaatgcaaaa aaagctgtct cattctttga
     1561 aacagcaatt aactcaatat agtaaaaaat caaaagttag caaaaaagta agcaaaccca
     1621 gaaatcaatg ttttaagata agacaaaaag cccactgttg taggctttct gtaagatata
     1681 tcttaaaatt aaagcatttt gttgtagaat tcaacgacaa gtgcttcatt gatttctggg
     1741 ttaatttcat cgcgttctgg aaggcgagtc aatgaacctt caagtttttc agcatcaaat
     1801 gatacgaaag ctggacgtcc taaagtagct tcaacagctt caaggattgc aggtactttc
     1861 attgattttt cacgaactga aataacttgt cctggagtaa cgcggtatga tgggatatca
     1921 acacgtttac catcaacaag gatatgacca tggttaacaa attgacgagc ttggcgacga
     1981 gttgttgcaa gtcctaaacg ataaacaacg ttatcaagac gacgctctaa aagaaccata
     2041 aagttgaaac caagagttcc ttcttttgct ttagtagctt gtacgaacaa gttacggaat
     2101 tgtttttcac caagtccgta tgaaaaacga agtttttgtt tctcagctaa ctgcaagcca
     2161 tattctgaaa gcttgctacg gttattagga ccgtgttgac caggtacgta gttacgacgt
     2221 gccaattctt tacctgtgcc tgtaagtgat aaaccaaggc gacgtgattg tttccatgat
     2281 ggaccagtat aacgtgacat ctaaatgtcc tcctctaaaa taattgagga aataacgatt
     2341 taaaaaatcc tgattcgtgc aggggatttt cgcctaaaca gcaaagggta ctagattagt
     2401 taaccacctg ttgacgagct tcatattttt ctgctgttat ttcacacaag ctatattata
     2461 tcatgaaaat aatattgaat caagttttta tgtctcttaa accttaaata caaatagaca
     2521 tctaataagt atttccaatt ataataagaa agattcttaa ttctaaccac caatagagaa
     2581 ttagtaaatt tttttataga aacaaaaaac ctcagtcgag gttttcatac tattctgctg
     2641 actcgtcatc aaaataacga attaaagttt tctcagttaa aatatctgaa taagtgtaag
     2701 actctacata agtattatca attgctcctg gtacagctgc agtatcctta tactctacga
     2761 taaacaaaga tgggtaaact tcaattaggc gcccaatttt atttttttcc ctcttacgac
     2821 cattttcgag agtaagttcg accatctgtc cctcatgtga cttgatatct tcttttattt
     2881 ttttcatttt tgcaacatct gcaaatgcat cactcatatt aacatctcct attcttcaaa
     2941 ttgagctata ctagaaaatt tattatattc cttttgaaac attaatttta ctgtcccacg
     3001 cgcaccagca cggttctttt ctaaaataac ctcaacagta ttatcttcta caatttcctc
     3061 tgcttcttcg ccctctctac gataataatc atctcgatat aagaaagcaa caatgtcagc
     3121 atcttgttca atcgatccag attcacgaat atcagataag accggtcgtt tatcctgcct
     3181 ttgctcaacc ccacgagaga gctggcttaa tgcgataaca ggaaccttta attctttagc
     3241 taaaatcttc aactgccttg aaatttcaga aacttcttgc tgacgatttt ctggtctagt
     3301 tcctgaaatt aattggaggt agtcaatgac gattaaacct aaaccatcat caacttcttg
     3361 agataacttc ctagagcgtg ctctaatttc tgtaatttta atcccgggcg tatcatcaat
     3421 ataaattggc gcatccgcca aagctccttg agcaatcgtt acattattcc aatcttgatc
     3481 cgtcaactga ccagttcgta aactatgaga atcaaccatt ccctctgctg ccaacatacg
     3541 gtcaactaaa ctctcagccc ccatttcaag tgaaaaaata gctacaggtc tattttgttt
     3601 agttccaaca ttttgagcaa tatttaaaac aaaggctgtt ttacctactg caggacgagc
     3661 cgctaagatg attaattgat ctggatgtaa acctgtagta atcctgtcta aatctctaaa
     3721 tccagttggt aaacctgtga catctgaggt ttgctgtgaa cgtaattcta agttttcgta
     3781 attaactttt aaaacatcag atatctttct aaacccgcta cgattactgt gttcgttaat
     3841 atcaactaaa gccttctcag cggtggcaat aatttcatca gaatcatttc cttcataggc
     3901 catgttgaca gtatctgtca acttagaaat gatatctcgc aacatggctt tctcagaaac
     3961 tattttggca taaaactccg cattagcact tgttggaaca ctattaacta attcaacaat
     4021 atagcccaac ccaccaatgt tttgaagatc accttggtca tctaaaatat ttctaacagt
     4081 agcagcatca attgcatcat ttctatcagc taaggtaatc atcgctctga agataacttt
     4141 gtgtgaatat ttataaaaat catctggact tataaattct ctcaccataa tcagtttctc
     4201 tggtgagata aagatagatc ctagaacggc ctgttctgcc aacaggtctt gaggttgtac
     4261 tcttagctca gatacttcta ccaaacttca ttcctcctga cttgtcaacc tttatcatct
     4321 ctcagtttac gcttctttga tatcaagctt aatctgtgaa gaaacctgtt tatgtaattt
     4381 tactggtact tcaaccttac caatcgctct aatagtatga tctaaatcaa tatgacgttt
     4441 gtcaatttta atgccgtatt gtttttgtaa ctcttcagca atctttttgg ctgtgataga
     4501 accaaacgta cgaccatctg gacctacctt ttcaataaat tgcactcggg tagtttcgga
     4561 ttctaattgt gttttcagct cctttgcctg tgctaaaatt tctgcttgtg ctttttcttc
     4621 ggacttttgt tttcctttta attcaccgat agcttgtgtc gttgcttctt tagctagatt
     4681 cttttttaat aagaagtttt gagcataacc agtaggtact tccttaacct cacctttttt
     4741 tcctttcccc ttaacatctt gtaaaaaaat aactttcatc tcaattaatc ccttctattt
     4801 tcgactgtac tattttctct taaatcttcg tcaattattt ccagtaacat acgtctaact
     4861 tgctttacag acttgtcttg tatttgacaa gcagcaaaac taaaatgtcc gccaccacca
     4921 agtttttcca taatacgctg gacattgata ttatttctac tgcgcgctga aataccaata
     4981 tttgaactgt ttttagtaat aacaaaagta gcctcaatac ctgccattgt caacatggtg
     5041 tcagctgctt tactagctat aacatgcgag taaactttat gttcttctcc ggtagcaaca
     5101 atgatgttat cataaatacg ctcacctttt aaaatcagtt cattaattaa acgatattca
     5161 tcaaaatctg tagctgatat tttttgaata gccatactat tactacctaa accgcgtaaa
     5221 tagcttgcca catcaaaagt tctactcgtt acattagatg caaaatttct tgtatcaagc
     5281 atgataccag ccattaaaat acttgcttga ctgcggctta atttatcttt agcttgttga
     5341 aattgaatca attctgtaac taattcgctg gcactacttg cgccactttc tataaatgaa
     5401 agaacagcat tttttggaaa atcttcatct cttctatgat gatctaccac aataacatct
     5461 gcaaacttgt taaagaattc cttagataag gttaaagctg ttttagaatg gtctaccatg
     5521 actaataaag aattttgagt aattaattca aaagctctct caacacttac gagacgtgtt
     5581 tcgccgtctt cttgcaagta atcaattgct ctttcaacat cagaattcat gtcatttggg
     5641 tcataaacta cataactggc attaacaata ttactagcaa aaaattgcat ccctacagat
     5701 gcacctagtg catccatgtc caatttcctg tgtcccacaa taaagacact atcaactact
     5761 ttcaaacggt cagaaatagc agtcatcata gctctggtac gtgttcgtga acgtttaata
     5821 gttgaaacag caccaccacc aaagtaaagt gcttttttac tactatcatt ttcacgtact
     5881 actatttgat ctcctccgcg aacgagtgct gtattaagat tttctaaagc aatttgccca
     5941 atttgattat ggttaccatc accataagaa attcccatac ttaatgttaa tgataaatgg
     6001 ttttcctgag cgcgttttcg aaactcattt aaaatatcaa atttatcttt tatcaatgta
     6061 tttaaaacgc tatagtcggt aaaaatatag tatcgatcca tattgacacg acgataaaaa
     6121 atatttttag actgtgtaaa atcagaaata aaactagtca cgaaggcgtt aatctttgac
     6181 atatcagcct ctaacatagt atccataata tcatcgtagt tatcaatgga aataatacca
     6241 ataactggac gtagcattga actatcatag tttcttctat tccccatgaa attatcgaag
     6301 aaatagaaaa cacccgtctc tgtatccaaa taagctgaat atttgttatc accgtattca
     6361 aatgattgcc cagccgttcc attacgtcta ctagtaatga tatctttaac agtagcagac
     6421 tgaaaatgac cattatcccc agtaaaaatc aattctgcat aagggttaaa ccactcaata
     6481 tcatttgttt caagatcaaa ttgtataaca ccaactggca tttgttcaag aagactctta
     6541 agagaaactt ctgtttgatc attaagtaat tcaatttgtt caacttcaga tagttcataa
     6601 gttatctttt ggtaatataa taaggcaaca acaaaagata aagcaacaaa aatagccaat
     6661 aatagagccg tataggattg aaataaccta acacaaatag ctaatagtcc aaataaaatc
     6721 aggcctatta aaactaaatg gacggttgca aaacgaaatc ttttcattaa ataacctctt
     6781 aagacctaat tataacacaa aacgcccctg ataaacaggg gcgaacacta ttatcaattg
     6841 atatttttac gacctttttg tcttccttca aggtaaacca ttaaaatact aatatcggct
     6901 ggattaactc ctgaaatacg actagcctga cctagcgttt ctgggttaat ttttttgaat
     6961 ttttgtctag cttctgttgc gatagaatca atgtcgtccc agtcaatatg agggggaata
     7021 cgtttttctt ccattcgttt catttttgca acttgatcta aagccttttt aatatagcct
     7081 tcgtatttaa tttcagtttc caaaagttca ataaccttac tgtctaatgg ctcatcagca
     7141 caaccaacaa aatccgttgc tactgcatat gtgatttgtg ggcggcgcat aaattctttg
     7201 gcagttaaag catctgtcaa aggtttaaag cccaattctt gaatccgttt atttgtctca
     7261 gaaactggct ttaactttat agagtctaaa cgttctaatt cattttcaaa ttgcatttga
     7321 cgctttttaa atatcgcata acgctcttca tctactaggc caatctcata accgatttct
     7381 gttaaacgca tatctgcatt atcatgacga agaatcagac gatattctgc gcgacttgtc
     7441 aacagacgat atggttctag agtccctttg gtaactaaat catcaatcat gacaccaatg
     7501 taagcatcac ttcgttttaa aataagttct ggcttccctt gaactttaag agcagcgttg
     7561 atcccagcaa ccaacccttg accagcagct tcttcataac ctgaagtacc attagtctgt
     7621 cctgcagtaa acaaaccagc aattactttt gtttcgagtg ttgctcttaa ttgatgaggc
     7681 aatacaatat catactcaat agcatagcct gtacgcatca tttcagcatt ttcaagccct
     7741 tttatagaac gtaataaatc aacttgaaca tcctcaggaa gactcgttga tagcccttga
     7801 acataaactt cttcagtgta tctaccttct ggttctaaga agagttggtg gcgctcttta
     7861 tcagcaaaac gcacaatttt atcttcaatt gacggacaat atctaggacc aactccctta
     7921 acgattccag aaaacatagg agcacggtgt agattttgat tgatgatgtc atgactcaaa
     7981 gtattcgtat aggtcaacca gcacggtacc tggtcagtaa tataatcttc atccctagac
     8041 ataaatgaaa aatgatttgg ttgctcatcc ccaggttgta tctctgtctt ttcataatta
     8101 atagaggagg cttttacacg aggaggtgtt ccagttttga aacgaccaat ttctaatcct
     8161 aggtctctta aattatctgc caatgtcaca gaagctaaac tattattagg gcccgaggag
     8221 tacttaagat cacctaatat aatctctcct cgaagagcag tacctgttgt tataacaaca
     8281 gattttgctg aaaatttctg attagttgct gtacgaaccc ccacaacctt tccatcttca
     8341 acaagtattt cgtcaatcat agcttgtctt aaggtaagat tttcttgctt ttctacagtt
     8401 tgcttcattg tttgtgcata gagggcttta tcagcttgtg ctcttaaggc acggacagca
     8461 ggccccttac ctgtattaag cattttcatt tgtatatagg ttttatcaat gtttttaccc
     8521 atttcaccac ctaaagcgtc aatctctctg acaacgatac ctttagcaga gccacctata
     8581 gaaggattac aaggcataaa tgctaacatt tctaagttga tagtggctag aagtgtctta
     8641 catcccatac gactagcagc tagactagct tccacacctg catgacccgc tccaacaacg
     8701 ataatatcat agttttctgc aaaattatgt gtcattttat tcctttccaa tttcaaagtc
     8761 taattcatta aaacacaaaa atagccaaaa ctctcgaaaa ttgtacgtca aaaagactta
     8821 caaattccat gagctttgac catcatagtt attgttaaat ttattttagc atttatcttt
     8881 taaaagtcaa ccagagggga taggctatgt taaatgaaaa ccaattttta tgcctgttat
     8941 tagcatctct atcccgatag tcatcaaaat aagtcccatc attttggtga ttatagtcat
     9001 tgcatttttt ccaagaaatt tagtaatttc gtttgcactt attaaaataa catacattat
     9061 cacacataat atggcaaaag caagtatagt aatgagtttg ccagataaac caccacttga
     9121 taaccccata gcagttgcaa tagtacccgg acctgccaaa aggggcattg ctaatggtga
     9181 aactgccaca ttcattggat catccttatg ctcctctagg tttttattgg ttggtgaatg
     9241 tataccatta atcattttat aacctataat gcctaccaat attccaccag atatttttag
     9301 ggcggctagg gtaatcccaa ataagttaaa aagtaagtgg ccacttaata caaagattac
     9361 aataatgaca aaagcaagta atactccttt tgctgcaatc cgacgtgaaa tcttctgatc
     9421 atcatcagct actaaagcca taaaagctgg caaattagaa atgggattca taatggcgaa
     9481 aaaagccatg aaagctaaaa taaattttga aatcatacga ttacctcact taataattaa
     9541 gtgtcaatta aatgtattga cagattttat catcacgata agcctgatca atcatcccac
     9601 cgcctaaaca ttcttgttca ttataaaaaa caacagcttg acctggtgtg attgctcgtt
     9661 gtaaatcatc aaacacaacc cttgcttgat taccttttac gtaaactgta actttactgt
     9721 caggttgacg atatctgaac ttagctgtac attctagctt aaattcatta ggcatatcac
     9781 gtgtaaagtg gatttcagaa gctgtcaagc tagttgacat caatgagtca tgatagaaac
     9841 cttgacctac ataaagaatg tttttactta aatcctttcc aactacaaac caaggtttat
     9901 tatctccacc atgttgccca ccaataccaa ggccccctct ttgaccaata gtataataca
     9961 ttaaaccagc atgttcaccc atgtcacgac catcaacagt catcatacga cctggttgag
    10021 ccggtaaata ttgacccagg aaatccttga aatttttttc tccaataaaa caaataccag
    10081 tagagtcttt tttcttagcg gtcgcaagtc ctgcttcttc agctatacgg cgaacttctg
    10141 gtttttgtaa atgaccaagt ggaaataacg ttttttgcaa ttgctcctga gataattgac
    10201 ttagaaaata agtttgatcc ttattattat ctgctcctct aagcatatga actattccgt
    10261 tctcatcacg tgttacttgt gcataatgac ctgttgctac atagtcagca cctaatgtca
    10321 tcgcataatc tagaaatgct ttaaatttaa tttccttatt acacataaca tctggatttg
    10381 gcgtacgacc tgctcgatat tctgctaaaa aatattcgaa aaccctgtcc caatactctt
    10441 tttcaaagtt gacagagtag tatggtatgc cgatctgatc agcaacagca gcaacatctt
    10501 tataatcttc agtagccgta caaacaccaa attcatctgt gtcatcccaa tttttcataa
    10561 aaacaccaat aacatcataa ccttgctctt ttaaaagcag ggcagttact gatgaatcaa
    10621 cacctccact catgcctaca acgacacgaa tgtttgaatt atcagtcata agaattctcc
    10681 catcaaaaag ttttattttt aacttaggct tgaagggcca agttgttctt agatcaatca
    10741 tattgactaa aaaacactat aaaagtgctt atatattata gcactatttg ttgtcaagtt
    10801 gtatgttttt gttgtgataa atatttatta cattgcagtt tttttatctt tttatgatat
    10861 tttttagaaa taatctgtta taatgagaat gtacaaaaat cacgaggaaa aactatgaaa
    10921 catttaaaat ttcaatcggt cttcgacatt attggtcctg ttatgattgg accatcaagt
    10981 agtcatactg caggagctgt ccgcattggt aaagttgtcc attctatttt tggtgaacct
    11041 agtgaagtaa cctttcattt atacaattct tttgctaaaa cttaccaagg acacggtact
    11101 gataaagcat tggttgcagg gattctagga atggatacag ataatccaga tattaaaaac
    11161 tctcttgaaa ttgctcatca aaaaggaatt aagatttact gggatatcct aaaagatagt
    11221 aattcaccac atccaaatac tgcgaaaata actgttaaaa atggggatag gagcatgagt
    11281 attacaggag tgtcaattgg tggcggaaac atccaagtta ctgagttgaa tggattctca
    11341 gtttcattaa caatgaatac tcctacttta attatcgttc atcaagatat acctggcatg
    11401 attgctaaag taactgatat tttatctgac tttaacatta atattgctca aatgaatgta
    11461 acacgcgaaa gtgcaggaga aaaagctatc atgattatag aagtagattc acgagattgt
    11521 caacaagctg tcaaaaagat agaagctatt cctcatttac acaatgtcaa cttctttgat
    11581 taagagaaag ggtaactatg ttttatacaa ttgaagagct ggtagagcaa gctaatagcc
    11641 aacataaggg taacatagca gagctcatga tccaaacgga aattgaaatg actggtagaa
    11701 gtcgtgaaga aattcgttat attatgtccc gaaaccttga ggttatgaaa gcttctgtta
    11761 ttgatggatt gacccctagt aaatcaatca gtggtttaac aggcggtgat gctgtcaaga
    11821 tggatcaata tttacaatca ggaaaaacta tttcagatac cacaatccta gctgccgtta
    11881 ggaatgctat ggctgttaat gagttaaatg ctaagatggg actggtctgt gcaacaccaa
    11941 ctgcaggtag tgcaggatgt ttaccagctg tgatttctac agccattgaa aagcttaatt
    12001 taacagaaga agagcaactt aattttctat ttacagccgg cgcatttggt cttgtcattg
    12061 gtaataatgc ctctatctca ggtgcagaag gaggttgcca agctgaagtt gggtcagcta
    12121 gtgctatggc tgcggctgct ttagttatgg ctgctggagg tactcctttc caagctagcc
    12181 aagctatagc atttgttatt aaaaatatgc ttggacttat ctgtgaccct gttgcaggtt
    12241 tagttgaagt cccttgtgtg aagcggaatg ctcttggatc aagttttgca cttgttgctg
    12301 ctgatatggc cttggctggt attgaatcgc aaattccagt agatgaagtt attgatgcaa
    12361 tgtatcaagt tggatcaagt ttaccgactg cttttcgtga aactgcagaa ggaggacttg
    12421 ctgccacgcc gacaggaaga cgttatagca aagaaatttt tggggaataa agtttatcaa
    12481 ttttctactc tttccctgaa aataaataaa aacgctacag ctttactgta gcgtttttat
    12541 ttatttgttt tagcaatctg taattattat tgacaatagt gtaaagttaa tttatagcta
    12601 ttcactagta accccaagct gataaacctt gagcacgata agctttaata gctgaattaa
    12661 cttgatcctg aactgtagct gttgaacccc aacctggcat cgtttggaaa agtcctgaag
    12721 ctcctgaggc attagcaaca ttaggattac catttgattc acgggcaata atatgttccc
    12781 aagtagactg agggactcct gttgcagcag ccatttgtgc tgcagcagca gatccgaccg
    12841 cccctgcagt atttccattg ctcaatactt ggccacttgt ctggtgttga gcaggtttgt
    12901 aagttgtctc agtaacagca taagtttgtt gtgcctgact ggtagcaggg gtattttctg
    12961 ttacaactgc ttgttctaca gccgcctctt cactcgcagt aacttgttgc tgagaattag
    13021 cttctgtaga ttgagaactt gattttgggg cttcattaga tgcctgagaa ggttttggag
    13081 cctgttttac atcttctact tttgatttag atgtcgcctt agtcattttt gattttttgg
    13141 ctacgcgaac tttatctgct tttgacaaag attcagtagt aacttgtgaa cttttaaaga
    13201 tgaccgttac agccaaaata catgaaaaaa gggctactaa agtgatagct ccataagcta
    13261 aatgttgttt ttttacattc aattttgata attttcttct tttattcatg ttacctatat
    13321 tatactttat ttgttacctt gcaattgaat agatgttaaa aatattacaa agagatttaa
    13381 ttttaagtaa atctctttgt tttatcaact tttattaata ccaaccgtta ctattccaaa
    13441 atgatagcgc tgccgaccaa gatccgtaac gagaagccac ataattgtcc gctacttttt
    13501 cttgattttc aggagataag tcgccattta ggtaagattg agacagttga tatcttccat
    13561 aatattgtcc attctgtgca gtataactac catttgattc acgacgagct atttcttctt
    13621 ttgcagctga atcacttgaa ctcaaatttg atgaagaatt cattgaattt tcaattgttg
    13681 gttgagattt tgcttctgct tgactagctg tagaattatc caattttaaa acatcaccta
    13741 tactgatgac atcagcgtta ctgatactat tgagagacac taactattgt accgtagttt
    13801 tatgattttt agcaatagct gataaggtat cacctgattt cacggtataa gatgctgcgc
    13861 ttactgaaag tggtaaagct aatgaagcac ccactaaaag acctgccact cctaatttaa
    13921 atggttttga ttttttaacc gaggttattg acataatata tgctctcttt cattaattga
    13981 tacgcgtact atcatacctt gtaaatatta ctaattgttt tgccttagat gaaatttatt
    14041 acaaataaat tgtaaattaa taaagaaaag gcctccaaaa aacaattttt agttttcttt
    14101 aagtaaagtt gttgagcttt tcacattata taaatgaaaa ggctaagacc ataaactgac
    14161 cttaaccttt taatttttta atagaaacaa actcaaaccc aataataaca tcagtaatat
    14221 actaaaagta tcccgaacag tccacttcaa taaacggtac ttactacgat tggctccacc
    14281 ttgataacct cttgcttcca tagcaattgc caacgcatct gctcttttaa aactagaagc
    14341 aaataatgga atcaaaatag ggataatgga cttaacctta tgaattaggt ttccctctcc
    14401 aaaatcaact ccacgagctc tttgagcatt catgatacgt gttgtatcat ccattaaagt
    14461 aggaacgaaa cgtaagctaa gggataacat caaaccaatc tcgtgtgctg gtactctcag
    14521 tacttccaat ggttttaaca aagattcaac agcatctgct aaacttaacg gagtagtagt
    14581 taaggtaagt aatgtcgaaa agaagataat taaaacaaat ctcataaaaa taagtgctgc
    14641 ttgttgtaac ccaaggctag tgatgctaaa aatccaaaac gagaagataa cctgcccgcc
    14701 ttgtgcaaac agcatctgga acagggttgt aaataaaata ataccaacca tcggtttaat
    14761 accattgaaa aaataggaaa attttatctg agataaaccc acaataacca acgtaaaaat
    14821 gaatacgatc acatttgtca ctacattatt tgcccaaaaa acaataatga taaaaagcaa
    14881 catggctagt aacttacttc gaggatctaa tttatgaatg agagaattac ctggtatgta
    14941 acgtcccaaa atcaatttat ccatgcttaa tagcctccac aaattcgtta atagtaattg
    15001 gtaaactagg taaatttaat cccttatgag atagtctttg agcaaacttg gtgattttgg
    15061 gaactcctaa ttgtttactt tctaaaagtt ctacttcttg aaaaatctgt tttggttgtc
    15121 ctgataaggt tactttccct gcttctaaaa catacacata gtcagcataa tccgctacat
    15181 cgtccattaa gtgagtcact aagacgatag tcattccttt tttatgaaga tttttaaaaa
    15241 gagtcattaa ttcttttctt cccttaggat caagtccagc tgttggctca tccagtacta
    15301 gtactttggg ttccatcgct aaaataccag ctatagcaac ccgcctcatc tgccctccag
    15361 aaagttcaaa tggattttta tcgaataaat cctcactgat accaactaac cttaattttt
    15421 cttcagccag cctttcagct tcaatctgag aaataccaaa attttgtggt ccaaaagcaa
    15481 catcctttaa aactgtctct tcaaaaagct gactttctgg aaattgaaaa actaaaccaa
    15541 ctttttgcct tataaatttg atttctttgt tcttgtcccc tgctttaata gaaaaatcat
    15601 cgacaattac ctcacctttt gtaggaatat gtaaaccatt caaaagttgc ataatagttg
    15661 attttccaga acctgtgtgc ccaatgaacg cggtatagga aacatcttca attttcagat
    15721 tgacgtcaaa aagggcacgc ccttcaaaag gagtgccggc ttgataggta taacttacat
    15781 ttttaaattc aattcccata attgttcttc taattccttc tctgtaagat actctgtccc
    15841 tatatcaaga ccattagcag ccagttctgc cattaaccta cttgtaaagg gaacatctaa
    15901 acctaaacta atcaaacgat ttccacgacc aaataacgct tttggagtgg atgtcgactc
    15961 aactttacca tttttcataa cgatgacacg atcactcaat gcaacttcat ctaagtcgtg
    16021 tgtaattgaa ataacagtaa gattatattt ttgacgaata gctcgtatcg ttcttatcag
    16081 ttccaatcgt ccctcaggat caagcatact agtcgcctca tccaagatga taacttgtgg
    16141 acgcatagct acagctcctg caatggctac acgctgcttt tgtcccccag ataaacgtgc
    16201 aggctccctc attttaaatt cagacatgcc taccaaatca agtgcttggt ccactctttc
    16261 tttcatgtct ttcaaaggaa tacctttatt ttccaaacca aaggcgacgt catcttcaac
    16321 agttgcccct acaaattggt tatcaggatt ttgaaatacc atgccaatct tgtgacgtaa
    16381 ctcccagaca ttatcttcgg ttaactcttg tccatcaatg ataatctggc ctgactccgc
    16441 ctctaaaagc ccatctatta aacgaacagt agtggatttc ccagaaccat tatgaccgat
    16501 aatagatagc cattctcctt gtttcacatg aaacgataca ttctccaatt gataatgtgt
    16561 ttgattacta tcatatttaa aaaagagatt attaacagta ataatatttg tcattattta
    16621 aaagtatcct taaataagaa gcctgcacct ttaaaatagt cgtagcctga atacaaggta
    16681 aagaataaag caatgtataa catgatgtta ccaagtgtca tccaatgtac taacaaaaaa
    16741 ataactgcaa acatttgagt agccgtcttt attttccctg gcatagctgc agcaagtact
    16801 gttccacctg tttcaactaa aagtaaacgc aaacctgtaa ctgctagctc tctacaaatg
    16861 ataatagctg aaacccaagc cggtgctaag tctagtccaa ctaacatgat aaaagcactc
    16921 ataactagca ttttatcagc taatggatct gcaaatttcc caaaatttgt tactacttgc
    16981 catttacgtg ctagataacc gtctaggtaa tcggtcaagc ttgcaatagc aaatacaata
    17041 gctgcaacaa tatgccaagt tgtacttgtt gttactgaag ttaatactat aaataacgga
    17101 atcattaaaa ttctgactac agttaataga ttcggaatat tttctttctt caccataaat
    17161 tctccttttg gaaacatagt attcctatat tatgttattt caaaactagt aaaagacttt
    17221 tgacatacca actttataga tgtctaagct tccttattat atcaaaattt cagattattt
    17281 acgaagattt tggaaaaata acgaagtcag atgtgtcagc attatctaag aaatccttac
    17341 caatcgtaat gacatcttcc aagtctaatt cttcaacgat agatggcaaa tctaaataca
    17401 tcgtttttcc atcatataaa taggtgacga attgcattgc gagattttca atagaatcta
    17461 agctccttaa aaaatcgcca tagagctctc tcttaacgtt ttgtaagtgg ctttcggtaa
    17521 gatcagatga ttgcaaagca ttttttagca ataacctcaa ctgagttgaa aaagcaattg
    17581 gctctgtcgt atctaaagaa ataataacgc actcaaaatc tggatgaact tcaatttcta
    17641 tatcaaaaga gtcatcaatt tgaccagatt cgtaccagtc ttgattaatt gtagaagtcc
    17701 atcctaaaag cattgcaaaa aatagctgta atccgatttt ctcttttagg taagaaccat
    17761 gtctcatatg acttgatttt cgataaccaa tagctaattt aggttttgtc actgtcatat
    17821 ggcaagagtt gttttttata acaggttcaa gactaataga atctcggtca atattctgtg
    17881 agtaatttgt taaatgagaa tttttttgaa ggtaagttat aatttcttgt ggactaaatt
    17941 gtccaacaag aactaaattc atattagaca actgataaaa gtctttgtga ttttcttgta
    18001 aatcggttaa ggttatttga gagattgatt ggtaatctcc agcgatatca aatgctaatg
    18061 gagagttggg gtaaaggttc gataacactc cttgataaag cctatattct gggtcatctt
    18121 gatacatttc aatttcttgt ttgataatgt ctttttcttt agtaattgac tcttctgtaa
    18181 agtttgttgt caatacaaag tcaagtagta tatctaagca gtttgtaatg tgacttatag
    18241 ttgaaaaata aaaactagtt ttatcaaaag ttgtaaaggc attactctcg gcgccatatt
    18301 tagtaaattg agttgcagcg tcttgtcctt tatccagttc aaataattta tgttctaaga
    18361 agtgagcaat tcctgccgga taatgttcta cacaaccatt cctcgtatat cttgtatgga
    18421 gtgatccaaa atttgctgtc aatactccaa cagtttcctt aaatgatggt tttggaataa
    18481 gatatacatt tagcccgctc tctaatgtta acttatatac ttcctcctga aggttctgat
    18541 aagttatttt attaactgtc atcctagttt ccttccaaaa aataaaaagc ttgtaatttt
    18601 aattttgttg ctacgctgac aatatctgat tttgttacat cgtccacttt gtcaatgaat
    18661 agtgctgaag tatgaaactg atctaaataa tgttcatgat aaatgtggtc aactatgacc
    18721 ttaggattat cagaagctaa aacatagttc attttcaata tatcttttgt ttgtttcaat
    18781 aatgtagaag agaacctgcc catcttaata ttattgaatt gcttattaat aagcttcaaa
    18841 aaacgctcac gattctcttt atcaatacct gcatatatcg taaagagacc tgtaaagctg
    18901 tcaaattgac tacctatagt ataggctaaa ccttgctttt ctcgaatttc tgtaaacaac
    18961 aaggaatggg caaaagcacc aaataaaccg ttaaatacta ttaaagcaaa ataatcttca
    19021 tctttataag ttattggaag atgataagcc aattgcataa tagattgatt aacgtcctta
    19081 tcttctactt gacttctcgt gatattttca taagtctgcg tataatcaaa agctaatact
    19141 ttgtgacgtg gctcaaatgc catacgatta aatgcctgaa tcattcgata gtcatcaaaa
    19201 tctcctacga caaaaatatc taattgatct tccttaagca tcttctgaaa ttcttgataa
    19261 gctgtaaatg aattttctga ttccactaaa gatgctgtac catacttagg taaacgaagt
    19321 gtcttattat tgtagaataa agattttagt gctaagtcac tgctataaaa attatcttca
    19381 atatcggcct ctaaatattg aataaggttt ttcttctcag tatcaaaaat agatgtttga
    19441 tactgttcta aagaaattaa aggagaaaac agcatatcct ccaagaatgt aataatttgt
    19501 tcaacgatat tttcttgttc taaagtaaag gtatttttaa caaatacaat atcaatatca
    19561 acaatgtgaa caagaccctt agtagaaatc ttagttgata aactagcacc atataaggaa
    19621 gctaactttt ctcgaaattc ttgtacctta gggtacttag catttgcagt aactaacatt
    19681 tgagcaacga gcgatctacg tgctactgtt ttattattaa aatcacctga aaatcgaaaa
    19741 gtaagatgat tagtcttaaa ttgttggttt ttaattaaat gtagagaaac gccttcaact
    19801 attttcattc aatatccttt gtgttctgta ttgttagcta agattatatc atttttaaag
    19861 gcgttaaaaa agattaaaca tccaagatat gattaattct aaatttcaat cttcttcaaa
    19921 attgacaaag tcgtcaacca atatttactc ccatttttta tgccttgtct ccatatcata
    19981 agttcaactc aaaaaataat tttatgctat aatgatgtga tatctaaaat tggagattaa
    20041 aaagtcatgg attataaatt atttgatgaa tatattactt tacaatcact tctaaaagag
    20101 attggtatca ttcaaagtgg cggagccatt aaaaaatttt tagcggataa tcaagtattg
    20161 tttaacggtg accttgaaaa taggcgtgga aagaaactcc gcctggggga tattattact
    20221 attcctgatc aaaatatcga gatcattata aggaagccat cagatcaaga aattgaagaa
    20281 cgtaatgtag aaatagctga aaaacagcgt gtttcagcaa tcgttaaaga aatgaataaa
    20341 aacaccaaca aagataaatc aaaaactagc aaaaaaccag ttcgctttcc agggacataa
    20401 aaatgtggat aaaaaatatt tctttaaaac attatcgtaa ttatgaagaa gcacaagtag
    20461 atttttcacc caacctcaat attttcattg gccgcaatgc tcaaggaaag acaaattttt
    20521 tagaagcaat ctacttcttg gctttgactc gaagtcaccg tacccgttct gataaagaac
    20581 ttgtacattt taaacaccat gatgtacaaa ttacaggtga ggtaatccgc aaaagtggcc
    20641 atcttaactt agatattcaa ttatctgaaa aaggaagaat cactaaagtt aaccatctta
    20701 aacaggcaaa attatctgat tatattgggg ctatgactgt cgtacttttt gcccctgaag
    20761 atcttcaatt agttaaaggt gcacctagtc taagacgaaa atttttagat atagatattg
    20821 gacaaattaa gtcaacgtac ttagctgaac tttcaaatta caatcatgta ttaaaacaaa
    20881 gaaacactta tctaaaaact actaataacg tcgacaaaac ttttttaaca gtccttgatg
    20941 aacaattagc tgattatgga agtcgtgtca ttgaacaccg ttttgatttt atccaagccc
    21001 taaatgatga agcggacaaa catcactaca ttatcagtac tgagttagaa catttatcta
    21061 ttcactacaa atcgtcaata gagtttacag ataagtcaag tattagggaa cattttttaa
    21121 accaattatc taaaagccac tcaagagaca tctttaagaa aaacacaagc atcggacctc
    21181 atcgtgatga tattactttc tttattaatg atatcaatgc tacttttggt agccaagggc
    21241 aacaaagaag ccttatttta tcattgaaat tagcagaaat tgagctaata aaaaccgtta
    21301 ccaacgatta tccgattctc ttacttgacg acgtcatgag tgaacttgac aaccatcgtc
    21361 aactaaaact cttagaggga atcaaagaga atgttcaaac atttattact acaacaagtt
    21421 tagaacattt aagtgcttta cctgatcaac tcaaaatctt taacgttagt gatggtacta
    21481 ttagtattaa tgaaaaaaaa gcaacagatt aatctgttgc tttttaataa gatttaacaa
    21541 taccaagtaa tatagcaccc ataacaaaac aaaggatacc catgacaacc catttctgct
    21601 ctttcttcgt ttttgtctca cctaaaaata aaataccacc aataatagag ataattactc
    21661 caagttgaga aaaactaaaa gcaattgcta gccctgcttt agctgctgcc aataacatga
    21721 agacattacc aacgccccac atgagacctg taatcatatt tttaacaaca acagcctcaa
    21781 agttaacacg aaacttcatg aaacaaattg cacctagaca cattccaaca gccatgggta
    21841 aaatgacggc catagcgtcg aacttcataa tgttgttaaa taaaactgcg tacgagatgt
    21901 aacctacagt tgaataagca attgtagcaa atccttttga aaactcttga tgtgttgcca
    21961 aagcttgttc tgaaacatca cgtttacttg agaaatagaa gccgataact aataatgtca
    22021 acgctgtcaa tcctaaaata aattggattg gcttagtcca ttcatgaaag actaaagcac
    22081 caactaggct tccacctact aattgtgcac cacttgacag tggattagca acagagactc
    22141 ccatatattt cattgcttga aattgaccat tttggccgac tgaccatagg ataccaccta
    22201 agataccaaa aatccacaat gaggcagtca tctctggctg tttaaataac catacgataa
    22261 tcgcaaatag caatgctcct aaagtcattc caaatgtttg ttgatttgga cgccctccaa
    22321 ttttattact aacaaatcca atacttcccc acgcaaacat gggaatcaat gcaataagta
    22381 aaccttccaa ttctttctcc ttacaatgga atattaagta ttacctattt tcaatcctag
    22441 agaatctatt gactaacttt attagcaaga tcagcaaata ctatagcttt atcaacacta
    22501 tctttttcca aagctataat gacttgtttt atcaataaat tgaccagtga tttagaataa
    22561 ggtaactgtt ctttagtcat attactaatc ttatttaaaa taacatcttc aggtaaaata
    22621 tcttgaaaat agcagatgac tttgaaataa aaatcattgt actgtgtcac accattcaga
    22681 taagtaacta ccaggttaat ctcatcttga ggcagcctca tatttaaccc taaatttgaa
    22741 ataaaaagtt tagccaatat tttgagaaca ttgctttcta ttctatcaca ggattgttgt
    22801 tcttggaaac tgaaaattct catgatatct tcattatctc gataagattc cagtaaagtt
    22861 aattgctcta aatcagtatt agaagttcca gccttaactt ttgaatctag aatggatact
    22921 tcattgatag ttaccccaac ctccaacgtt tgtaaagctt taataaattt atcaatacct
    22981 atttgtgtct caccattttc aaaacgtgat agagttgatt ttgataattg agcacttttg
    23041 gctagagaac ttagggatat acctttgtct tctctcaact cttttaaaat ttttccataa
    23101 tttttcatac aaaaacttta ctatatttgt ttcttttatg caacaaaaag catcctcata
    23161 ggggatgctt ttttgtcctt attttagtgt actgaataat ttggtgcctc atttgtaatt
    23221 tgaacatcat ggggatggct ttctttcaaa ccagctcctg acatttcaac aaattgagca
    23281 ttatcatgaa gttcttttat gttcgctgca cctacatatc ccataccaga acgaatgcca
    23341 ccaagcattt ggaaaacaat atcggctact gaacctttgt atgctacacg accttcaata
    23401 ccttctggaa ccaatttgtt agcctcgttt acagaacctt ggaaataacg atcgcttgaa
    23461 ccttttttca ttgctgcaat tgagcccata ccacggtatg tcttgaactt acgcccttgg
    23521 aaaatttctg tttctcctgg cgcttcatct gttcctgcaa acattgaacc tagcataact
    23581 gcatttccac ctgctgcaag tgcttttaca atatcacctg aatatttaat accaccatca
    23641 gcaatgatag tcttaccgta ctcacgcgca acagccgctg catcgtaaat agctgtaatc
    23701 tgaggaacac ctaccccagc aacgacacga gtcgtacaaa ttgaacctgg accaatacca
    23761 actttaacga catccacacc tgcatcataa agtgcacgcg ctccttctgc tgtagcaata
    23821 tttcccgcaa tcaatgtgcg attagggaag tgagcacgaa tttcagcaat tttacggagc
    23881 acaccagctg aatgtccatg tgctgtatca atgacaatag catctgcacc tgcttcaaac
    23941 aaagcttctg cacgttcaaa agtatcagag gttacaccta ctgcacctgc aactaagaga
    24001 cgtccaaatt catcttttgc tgccttagga aattcaataa ctttttcgat atccttaata
    24061 gtaataagtc ctgaaagacg tccctcatca tctactaaag gtagcttttc aatacgatgc
    24121 tcatgaagaa tacgctccgc agtttcaaga tctgtaccaa taggagctgt tacaagattt
    24181 tgacttgtca tgtgctctga gattagttgt ttataatcag aaatgaaacg catatcacgg
    24241 ttagtaataa ttccaactaa tttacgattc tcgagtgtct ctacaatagg aactccacta
    24301 atgcgatagt tttgcatcaa ttcctcagct tcagagacag tattatctgg tgtcaagaag
    24361 aatgggtcaa taataacacc attttctgag cgtttaactt tacgaacttc ttctgcttga
    24421 tcaactattg acatattttt atgaatgata ccaagcccac cagcacgtgc aatagcaata
    24481 gccatcttac tatctgtaac tgtatccatt gcagctgtaa tgattggaat gtttaatgtc
    24541 aaattatcag ccaactttgt attcatatca acttcatttg gtaaaacatg gctttcagca
    24601 ggaataagca gaacatcatc aaaagtaaaa ccttttttca aaaacttagt atcccaattt
    24661 gacatcaaaa aatcctcttt tcttagatag tagatataaa aaatcccatt aaaattatat
    24721 tgttactatc atatcacgct aaaatcattt gtcaatataa aaagagcata gaattaatag
    24781 cttctagctc tttaaagtaa tgaaaaaatc ttcttaattt tctatttttt tgagaagaag
    24841 aaaggcggcg tatactgact taatttctca aaacactttt gagcttcttc actattatta
    24901 caaataatca aacaagtatc atcaccacag acagttgcta agatctcagg taataaaatc
    24961 gaatctaata tggagccaaa agattgagct aagccaggta gtgtcttcaa aacgatttgg
    25021 ttttgaactg gaaacagcat gactagggca tcttccatgt aaaaacgtaa ccgttcttcc
    25081 caacgcttct gagatatact atgaatctca taataagttt ctgtagcatt atcactgctt
    25141 tcgttaacct taattaaatt aagttccttc atatctcgag ataatgttgc ttgcgtcact
    25201 gaaacgccat ttttttctag taattccctt aactcatgtt gtgtacgcac cttagtctca
    25261 gagactaagg aacgaattaa ttggtgtctt gtttcttttt tattcatata aactccttat
    25321 tcaatgaatt tcttgaaata atttttatta ttatagagaa gcttctttcc tttcatttca
    25381 acgatatctt tttggcgtaa ttcctttaaa acatgactaa ctgtctcgcg agtagttccg
    25441 ctcatattgg caatttcaat cgttgtaata ggaaaaggta aatgtcccct ttctgtagga
    25501 acttgcaaaa gtagagttgc aagcgattgg ataactctca tactggcact tgaagtcacc
    25561 atattacgaa gtcgtatttc atgaactcgt aatagcttag aatatttccg acaaagatgc
    25621 ttcatttgat tgatattttg aagagagtat tcttcgtata aagccattgg cagagaaaaa
    25681 tactcaatat ccgtaatagc tactgctgaa aaatggtaat catcatccaa aaagagacct
    25741 ccataaggaa aaactgtatc ttgtctaaca taatctgtat aaataaaaga accagaagcg
    25801 tctgattgtt caatttttac atgtcctgac tggattaaaa acagtttttc acggtaatca
    25861 ccttcaaaaa ataaggtatg attttttaga gctgtacgat gtttaatgtg gctcacaata
    25921 tggtcaaatt gttcaatagt aaagtgttta aaatcatcta gttgtctgaa ataaaaatat
    25981 tgttcttttg taatcacctg attttctaga cctttctaat cattagatgc gaacattttt
    26041 tgtataatga aaatttttt
//
LOCUS       DATROQ010000017        25727 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.017, whole genome shotgun sequence.
ACCESSION   DATROQ010000017 DATROQ010000000
VERSION     DATROQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25727
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.017"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..190
                     /locus_tag="VBC94_000674"
     CDS             <1..190
                     /locus_tag="VBC94_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978966.1"
                     /translation="RISALINAKFPQGKSSLAEKLHKELIWDLDGVKYDEGISKRTTL
                     KKLEGKLNEYTKKYSYLF"
     gene            complement(242..428)
                     /locus_tag="VBC94_000675"
                     /pseudo
     CDS             complement(242..428)
                     /locus_tag="VBC94_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            500..1225
                     /locus_tag="VBC94_000676"
     CDS             500..1225
                     /locus_tag="VBC94_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6978967.1"
                     /translation="MLKIENVTGGYVNIPVLKNISFEVNDGELVGLIGLNGAGKSTTI
                     NEIIGILRPYQGDITIDGISLEANQELYRKKIGFIPETPSLYEELTLREHLETVAMAY
                     DIATDEVMARAQKLLEMFRLTDKLDWFPMHFSKGMKQKVMIICAFVVSPSLFIVDEPF
                     LGLDPLAISDLINLLAEEKAKGKSILMSTHVLDSAEKMCDRFVILHKGEIRAVGTLEE
                     LRAIFGDSNANLNDIYIALTKEV"
     gene            1227..2261
                     /locus_tag="VBC94_000677"
     CDS             1227..2261
                     /locus_tag="VBC94_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6978968.1"
                     /translation="MKKLFNKRRSLFLTQNSKYLRYVFNDHFVLVLMFLSGFLLYQYS
                     QLLKDFPKTHWPIIVIVSIIILMLLAMGEIASYLEPADKQFLLIKEEAIKEIINSAKK
                     RTYIFWLVVQTLFLVLISPILIKLGLSVFMITLLIFGLGIIKWLVITYKVKVFYNNQN
                     LNWDAAINHEQERKQAILKFFSLFTNVKGISTSVKRRSFLDVILKLISKTPSRLWTNL
                     FVRAFLRSSDYLGLTIRLVTLNILSVIFVNETYLALALAFVFNYLLLFQLLALGHHFD
                     YQYMNQLYPVRLNAKASQLKGFLRVLSYAVTVIDIILIRELKPVILLIVLMLIVTEYY
                     IPYKIKKMID"
     gene            2319..3119
                     /locus_tag="VBC94_000678"
     CDS             2319..3119
                     /locus_tag="VBC94_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEO6978969.1"
                     /translation="MTISNQELTLTPLRGKSGKAYIGTYPNGERVFVKYNTTPILPAL
                     AKEQIAPQLLWARRTSNGDMMSAQEWLDGRTLTKEDMGSKQIIHILLRLHKSRPLVNQ
                     LLQLGYKIENPYDLLMDWEKQTPIQIRENTYLQSIVTELKRSLPEFRTEVATIVHGDI
                     KHSNWVITTSGLIYLVDWDSVRLTDRMYDVAYILSHYIPQKHWKDWLSYYGYKDNEKV
                     WSKIIWYGQFSYLSQIIKCFDKRDMEHVNQEIYELRKFRELIKKYNAS"
     gene            3109..3744
                     /gene="trmB"
                     /locus_tag="VBC94_000679"
     CDS             3109..3744
                     /gene="trmB"
                     /locus_tag="VBC94_000679"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEO6978970.1"
                     /translation="MRVRKRKGAEEHLENNPHYVISNPEEAKGRWHEIFGNNNPIHIE
                     VGSGKGAFITGMAEQNPDINYIGIDIQLSVLSYALDKVLDSGAKNIKLLLVDGSSLSN
                     YFDTGEVDLMYLNFSDPWPKKKHEKRRLTYKTFLDTYKDILPEQGEIHFKTDNRGLFE
                     YSLASFSQYGMTLKQVWLDLHASDYQQNIMTEYERKFSNKGQVIYRVEARF"
     gene            3788..3875
                     /locus_tag="VBC94_000680"
     tRNA            3788..3875
                     /locus_tag="VBC94_000680"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3822..3824,aa:Ser,seq:gga)
     gene            4233..4709
                     /gene="rimP"
                     /locus_tag="VBC94_000681"
     CDS             4233..4709
                     /gene="rimP"
                     /locus_tag="VBC94_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEO6978971.1"
                     /translation="MANQTIVDIVTQVVTPAIQAPFELVDVEYEKMGGDYVLSILIDK
                     PGGITVEDTAQLTDVVSPLLDTIQPDPFPEQYMLEVSSPGLERPLKTAEALSNAVGSY
                     INVSLYKSIDKVKIFEGDLLSFDGETLTIDYMDKTRHKTVDIPYQTVAKARLAVKL"
     gene            4736..5896
                     /gene="nusA"
                     /locus_tag="VBC94_000682"
     CDS             4736..5896
                     /gene="nusA"
                     /locus_tag="VBC94_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEO6978972.1"
                     /translation="MDTMSKEMLEAFRILEEEKHINKEDIIDAVTESLKSAYKRRYGQ
                     SESCVIEFNEKKADFTVYTVREVVDEVFDSRLEISLKDALAISSAYELGDKIRFEESV
                     TEFGRVAAQSAKQTIMEKMRRQMREVTFNEYKQHEGEIMTGTVERFDQRFIYVNLGSL
                     EAQLSHQDQIPGESFKSHDMIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEREIPEV
                     FDGTVEIMSVSREAGDRTKVAVRSHNSNVDAIGTIVGRGGSNIKKVISNFHPKRVDAK
                     TGLEIPVEENIDVIQWVEDPAEFIYNAIAPAEVDMVLFDDEDTKRATVVVPDSKLSLA
                     IGRRGQNVRLAAHLTGYRIDIKSASEYEKMEAQELQTEEVAQESEVISDKAE"
     gene            5918..6214
                     /locus_tag="VBC94_000683"
     CDS             5918..6214
                     /locus_tag="VBC94_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEO6978973.1"
                     /translation="MAKTKKIPLRKSVVSGEVIDKRDLLRIVKNKEGQVFIDPTGKQN
                     GRGAYIKLDNDEAILAKKKRVFDRSFSMEVSDEFYDELLAYVDHKVKRRELGLE"
     gene            6207..6509
                     /locus_tag="VBC94_000684"
     CDS             6207..6509
                     /locus_tag="VBC94_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEO6978974.1"
                     /translation="MNNSEKVLNLIGLAQRAGRLITGEELVIKAIQNQQVSLIFLAND
                     AGPNLTKKVTDKSNYYKTEVSTVFSTLELSDALGKPRKVVAVADAGFSKKMRTLMA"
     gene            6529..9312
                     /gene="infB"
                     /locus_tag="VBC94_000685"
     CDS             6529..9312
                     /gene="infB"
                     /locus_tag="VBC94_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEO6978975.1"
                     /translation="MSKKRLHEIAKEIGKTSKEVVEQAQSLGLPVKSHASSVEENDAT
                     RIVESFSSSKTKAPTNSVQTNQGVKTESKTVETKQGLSDDKPSTQPVAKPKPQSRNFK
                     AEREARAKAEAEKRQHNGDHRKNNRHNDTRSDDRRHQGQKRSNGNRNDNRQGQQNNRN
                     KNDGRYADYKQKPQTRPQQPAGNRIDFKARAAALKAEQNAEYSRHSEQRFREEQEAKR
                     QAAKEQELAKAAALKAQEEAQKAKEKLASKPVAKVKEIVNKVAATPSQTADSRRKKQT
                     RSDKSRQFSNENEDGQKQTKNKKNWNNQNQVRNQRNSNWNHNKKNKKGKTNGAPKPVT
                     ERKFHELPKEFEYTEGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGDTIELL
                     MVDYGIEAHAKVEVDEADIERFFADEDYLNPDNLTERPPVVTIMGHVDHGKTTLLDTL
                     RNSRVATGEAGGITQHIGAYQIEEAGKKITFLDTPGHAAFTSMRARGASVTDITILIV
                     AADDGVMPQTVEAINHSKAAGVPIIVAINKIDKPGANPERVISELAEHGVISTAWGGE
                     SEFVEISAKFGKNIQELLETVLLVAEMEELKADADVRAIGTVIEARLDKGKGAVATLL
                     VQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGDHFAV
                     YADEKAARAAGEERAKRALLKQRQNTQRVSLENLFDTLKAGEVKSVNVIIKADVQGSV
                     EALAASLLKIDVEGVKVNVVHSAVGAINESDVTLAEASNAVIIGFNVRPTPQARQQAD
                     ADDVEIRQHSIIYKVIEEVEEAMKGKLDPEYQEKILGEAIIRETFKVSKVGTIGGFMV
                     INGKVTRDSSVRVIRDGVVIFDGKLASLKHYKDDVKEVGNAQEGGLMIENYNDLKEDD
                     TIEAYIMEEIKRK"
     gene            9402..9770
                     /gene="rbfA"
                     /locus_tag="VBC94_000686"
     CDS             9402..9770
                     /gene="rbfA"
                     /locus_tag="VBC94_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEO6978976.1"
                     /translation="MKKGLIMANHRIDRVGMEIKREVNEILRLRVNDPRVQDVTITDV
                     QMLGDLSMAKVFYTIHSTLASDNQKAQIGLEKATGTIKRELGKNLTMYKIPDLQFVKD
                     ESIEYGNKIDEMLRNLDKKD"
     gene            complement(9842..10846)
                     /locus_tag="VBC94_000687"
     CDS             complement(9842..10846)
                     /locus_tag="VBC94_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6978977.1"
                     /translation="MKPSFKKLLLLFSIITILSIACTPHAKASGRSWKSWFIEQYFWL
                     KRDKSYYKVQDESSFQKYLNASREQSDKGYYLDPNSVNGGLVQERLFDMQVYSWNDNG
                     KANQKTIFYLAGGSYLNNPTPYHISMLKTLSTSLDAKIILPIYPKTPRYTYDYAIPRL
                     VNLYRHFHEKNANLTLMGDSAGGGLALGLAHALSHQSGQEAIPQPKNIILLSPWLDVT
                     MKHPEIPKYEDTDPILSAWGLARVGEIWANGSNNTNHTYVSPKNAPATKLAPITLFTG
                     TREIFFPDIRDYAAQLQAANHPVNYIAQEGMNHVYPIYPIEEAKTAQYQMIDIINKTP
                     "
     gene            11009..11425
                     /locus_tag="VBC94_000688"
     CDS             11009..11425
                     /locus_tag="VBC94_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEO6978978.1"
                     /translation="MTISNAEWEIMRVVWAQQNTTSNEILAVLLEKYDWTPSTVKTLL
                     RRLLDKGYVSREKMGKGFSYSSLIDEDLAMMSEVDSVLQKVCQTKHVAIVRHLLESIP
                     MTEKDRLNLQASLEAKKGKTLERVACNCIPGQCQCH"
     gene            11438..13672
                     /locus_tag="VBC94_000689"
     CDS             11438..13672
                     /locus_tag="VBC94_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEO6978979.1"
                     /translation="MANRKETFLIDGMTCASCALTIEKAVNKLDHVDSAVVNLATEKM
                     TVTFDDTTLSPNVIEECVSETGYEASLFKEETSKSQSERHQLAIEKMWHRFWMSAVAT
                     IPLLYISMEPMINLWLPSFLMPDKGPLNYGVIQLLLTLPVMYFGRIFYQNGFKALFKR
                     HPNMDSLVAIATTAAFIYSLYGLYEILQGDIHYAHQLYFESVAVILTLITLGKYFEIL
                     SKGRTSASIEKLLTLSAKEARVIKDGEDYMVPLDKVKIGETILVKPGEKIPLDGHVVA
                     GESSIDESMLTGESIPVEKKVGSKVYGASINGQGSLTIFVEKEAVGSLLSQIINLVEV
                     AQASKAPIANLADKVSGVFVPFVIVIAILSGLSWYLILGQSFAFSLKIMIAVLVIACP
                     CALGLATPTAIMVASGKAAENGILFKGGEVLEKAHHIDTIVFDKTGTLTKGKPEVVAI
                     KTYGVDKEEFLGQVASVEKLSNHPLSQTIVNKAKEKELPLREVMAFKNILGYGLSAII
                     NGKTMLVGNANLMTKNDVNLDLAKADIEIAQEEAQTVVYVSENGVLSGLITLTDQLKT
                     DSQETVKQLQRLGFNLVLLTGDNKASADAIAQKLGITTVVSEVLPDQKANVILELKEK
                     GGQIAMVGDGINDAPALASSDVGISMSSGTDIAIESADIVLMKPELTDLLKAMTISKQ
                     TIQIIKENLFWAFFYNVLAIPVAMGVLHLFGGPLLNPMLAGLAMAFSSVSVVLNALRL
                     KVLK"
     gene            13713..13919
                     /locus_tag="VBC94_000690"
     CDS             13713..13919
                     /locus_tag="VBC94_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEO6978980.1"
                     /translation="MKHTYRVSGMKCDGCAKTVSDKLSSVMGVDEVNVDLTKNQVVVS
                     GKTFKCLLKRSLKDTKYSLEEEIN"
     gene            14029..14643
                     /locus_tag="VBC94_000691"
     CDS             14029..14643
                     /locus_tag="VBC94_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEO6978981.1"
                     /translation="MSIDIWNILSLVGTVAFASSGAIVAIEEEFDVLGLFILGFVTAF
                     GGGAIRNVLIGLPIETLWSQGIAFYAAAAAILFIMIFPNLLSGKGRDAEVVSDAIGLA
                     AFSVQGALYATQSHQPLSAVIVAAVLTGAGGGIVRDVLAGRKPGVLRSEIYAGWSILV
                     GIILYFKIAKTTTDYYLLVLVVTSLRMLGYKKQWHLPVVRPRKK"
     gene            14658..15470
                     /locus_tag="VBC94_000692"
     CDS             14658..15470
                     /locus_tag="VBC94_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6978982.1"
                     /translation="MIKLIAIDMDGTLLNDEKKIPKENIQAIKEATQAGIKIVLCTGR
                     PMSGILPYFNELGLTKEEYIIMNNGCSTYSTKDWQLIDSATLTHDELIFLEEVVKEFP
                     NVCLTLTAENTFYAVGEEVPEIVAYDADLVFTKAKSISLDALRNQEEIVFQAMYMGLD
                     ADVTAFQEAVEEALISKFSGVRSQDYIYEIMPQGVTKARGLKSLIAKLGLDINQVMAI
                     GDAPNDIELLDLVPNSVAMGNASDEIKSRCKYITVDNNQAGVAKAIYDYALR"
     gene            15583..18225
                     /gene="polA"
                     /locus_tag="VBC94_000693"
     CDS             15583..18225
                     /gene="polA"
                     /locus_tag="VBC94_000693"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEO6978983.1"
                     /translation="MTNKNKLLLIDGSSVAFRAFFALYNQIDRFKNNSGLHTNAIYGF
                     HLMLNHILGRVQPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPYIRQQLD
                     VLGIKHYELEHYEADDIIGTLAKQAEASKEHFDITVVSGDKDLIQLTDTNTVVEISKK
                     GVAEFEEFTPAYLMEKMGITPSQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLSEYGS
                     LEGIYENIEAMKQSKMKENLINDKEQAFLSKTLATINIASPITIGLEDILYSGPQDIK
                     ALSQFYDEMDFKQFKAALGEETSQEDFEVDFTEVEQLKPEMFSDNDFYYFEMLGDNYH
                     VEDLIGIAWGNSDTIYATSDVSLLQEALFKKALSKPIKTYDFKRSKVLLNRFNIDLPE
                     PAFDTRLAKYLLSTTEDNLVSTIARLYTNLPLDTDDAVYGKGAKRAIPEKTRFLEHLA
                     KKVKVLVDSEANIMQQLKANEQEELLFEMEQPLANVLAKMEIRGIKVKKNTLNEMAIE
                     NQKVIETLTQEIYELAGQEFNINSPKQLGKLLFETLGLPVEMTKKTKTGYSTAVDVLE
                     RLAPISPLVTKILEYRQITKLQSTYIIGLQDYILEDGKIHTRYVQDLTQTGRLSSSDP
                     NLQNIPVRLEQGRLIRKAFVPSEDNAVLLSSDYSQIELRVLAHISKDEHLIAAFKEGA
                     DIHTSTAMRVFGIEKPENVTPNDRRNAKAVNFGIVYGISDFGLSHNLGIPRKLAKQYI
                     DTYFERYPGIKNYMETVVREAKDKGYVETLFHRRRSLPDINSRNFNIRQFAERTAINS
                     PIQGSAADILKIAMINLDRVLDKGGYKSKMLLQVHDEIVLEVPNEEIGAIKELVTKTM
                     ESAISLSVPLIADENAGETWYEAK"
     gene            18255..18695
                     /locus_tag="VBC94_000694"
     CDS             18255..18695
                     /locus_tag="VBC94_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEO6978984.1"
                     /translation="MVYHFQNLSDFMLKNYLTKAKTIAVVGLSDRQETAAYQVSKIMQ
                     EAGYQIIPVNPKNAGQKILGQMTYASLKDVTEHIDIVNIFRRSEYLPDIAREFLEVDA
                     DIFWAQLGLESQEAETILKQAGHKQIVMNKCLKVEYQKLHSETN"
     gene            18777..19256
                     /gene="perR"
                     /locus_tag="VBC94_000695"
     CDS             18777..19256
                     /gene="perR"
                     /locus_tag="VBC94_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEO6978985.1"
                     /translation="MDSHSHGHRPLDAYENVLEHLREKRIRITETRKAIISYMVNSRE
                     HPSAEKIYNDLLPEYPNMSLATVYNNLKVLVDEGFVTELKLCNYSTTYYDFMGHQHLN
                     IACEDCGKIVDFVDVDLLDISREAHQQTGFEVTRVQLVAYGICPECQRKRKDKYKKD"
     gene            19409..20974
                     /locus_tag="VBC94_000696"
     CDS             19409..20974
                     /locus_tag="VBC94_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEO6978986.1"
                     /translation="MKISQYNKWSIRRLKVGAASVMIASGSIVALGQSHIVSADEMSQ
                     PKTTITAPTANTSTNVESSTDKALSKVTTMETSSEMPKMQNMAKVEKTSDKPMMVATS
                     VRKMMATPTPVAMTKTTSVDEVKKSTDTAFKQTVDVPAHYVNAAKGNGPFLAGVNQTI
                     PYEAFGGDGMLTRLILKASEGAKWSDNGVDKNSPLLPLKGLTKGKYFYQVSLNGNTTG
                     KEGQALLDQIKANDKHSYQATIRVYGAKDGKVDLKNMISQKMVTINIPHITTDMEVKN
                     SLKMAFKEKVDVPAKYVSAAKAKGPFLAGVNETIPYEAFGGDGMLTRLILKASEGAKW
                     SDNGVDKNSPLLPLKGLTKGKYFYQVSLNGNTTGKEGQALLDQIKANDKHSYQATIRV
                     YGAKDGKVDMNTILGQKTVMIHINVAKKDMNSTSMMMKKDKMTMPMKKEMTSSKINTG
                     MMMSNNKMSANVQMSSQAKSNDKAGKKMSMMSKNLPNTGETKQQNVGVLGMLSLAFAT
                     GLTALGLKKSKQR"
     gene            21087..21773
                     /locus_tag="VBC94_000697"
     CDS             21087..21773
                     /locus_tag="VBC94_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6978987.1"
                     /translation="MTQKLLLVDDEFEIIDINRRYLEQAGYEVSVAADGIEALKEVDE
                     NRFDLIISDIMMPKMDGYDFISEVLVREPNQPFLFITAKVSEPDKIYSLSMGADDFIS
                     KPFSPRELVLRVKNILRRIYGNHQQSEVLTIGDLVIDQKQRLVMVDCNTISLTNKSFD
                     LLWILANHLNRVFSKTELYERVWGEEFLDDTNTLNVHIHALRNDLAKFSTDNTPTIKT
                     VWGLGYKLEE"
     gene            21775..22812
                     /locus_tag="VBC94_000698"
     CDS             21775..22812
                     /locus_tag="VBC94_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6978988.1"
                     /translation="MKLKYYIVIGYLISMLITVAGVFFGLNHMLIETRGVYYILSVTI
                     IACIVGGIVNLFLLSSVFTSLKKLKQKMKDISQRRFDTKAQICSPQEFKDLETAFNQM
                     SSELESTFKSLNESEREKTMMIAQLSHDIKTPITSIQSTVEGILDGIISEEEVNYYLN
                     TISRQTNRLNHLVEELSFITLETMSDTAEPHKEETIYLDKLLIDILSEFQLVFEKENR
                     QVMIDVAPDVSKLSSQYDKLSRILLNLISNAVKYSDPGSPLTIKAYSNRQVIVIDIID
                     QGYGIKDEDLASIFNRFYRVESSRNMKTGGHGLGLYIARQLAHQLNGDILVESQYQKG
                     SKFSLVLKLQK"
     gene            complement(22826..23566)
                     /locus_tag="VBC94_000699"
     CDS             complement(22826..23566)
                     /locus_tag="VBC94_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEO6978989.1"
                     /translation="MTNSEIKNEAKTILSNLQGKNQLFLLPILLSIITLYISFYYQYN
                     NMTLLDFFVPLPVYFFYTLFIISVSFVMLDVVKNQKLNVRFSDNTYVFSSHIFWKLLS
                     VLVLKGLILSFFYLLSTFGLLIIISSFRLLLSGNLILAPVLIVVSSLITTKAVIKLVQ
                     QYYSYSISTLVFYTQLESGNYEGPSKVLVASRELMNGNKLRLFLLDLSFIGWHFLTIF
                     SFGLVYIYLLPYQTTARLIFYRNITKNS"
     gene            23753..24895
                     /gene="tgt"
                     /locus_tag="VBC94_000700"
     CDS             23753..24895
                     /gene="tgt"
                     /locus_tag="VBC94_000700"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEO6978990.1"
                     /translation="MTDHPIKYRLIKQEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIAKAGGLHKFMNWDQAILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGAKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLNAHRRPHDQGLFGIVQGAGFEDLRRQSARDLVSMDFPGYSI
                     GGLAVGETHDEMNAVLDFTVPMLPNDKPRYLMGVGAPDSLIDAVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKFAEDFTPLDPNCDCYTCKNYTRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKDVRQAIMDDNLLEFRQDFMERYGYGTNNRNF"
     gene            25001..25312
                     /locus_tag="VBC94_000701"
     CDS             25001..25312
                     /locus_tag="VBC94_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978991.1"
                     /translation="MMQEYFGIGLDSSSRCYHYHTKLDIVALKCAVCQKYYACYKCHD
                     ALEEHCFVATKSDEMFPVLCGSCRQMLTLKEYKTGFCPYCRMLFNPNCQRHDSIYFSK
                     E"
     gene            25319..>25727
                     /locus_tag="VBC94_000702"
     CDS             25319..>25727
                     /locus_tag="VBC94_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEO6978992.1"
                     /translation="MFHTKSLIQMAMMATLLVVLGFIPALPLGFIPVPIVLQNLGVMM
                     AGVILGGRKGSLSILLFFLVGLVIPVFSGLRSTIPVLTGPTAGYVIAWLFVPLLISYG
                     VKLVNKKNFIAIFLIVWLAGVLFVDVAGAVWLAS"
BASE COUNT         8693 a         3911 c         5195 g         7928 t
ORIGIN      
        1 aagaatttcg gctcttatta atgctaagtt tccgcaaggg aaatctagtt tagcagaaaa
       61 attacataaa gaactaattt gggacttgga tggtgtcaaa tatgatgagg ggatttctaa
      121 gagaactact ctaaaaaaat tagaaggtaa actcaacgaa tatactaaaa aatacagtta
      181 tttattctga taattgactt tggtaatttt atagcctaat attctgatta ctttgcattt
      241 attatattga tactaaaaag acaccttttt gaggtgtctt tttttgtccg atgagtgatg
      301 gaggtgcttt ttgtaaacat aatgggagca tggggtagct tggcatttca attagatgta
      361 aaagcggaca aaataggttt aaagttattt tcaaaactat aagggaattc taagatacaa
      421 ttatccataa gaatagacct tccctttctt attttgttat aatagttaat aaagtatatc
      481 aaagatagag agaatgacaa tgttaaaaat tgaaaatgtt acaggggggt atgttaatat
      541 cccagtttta aaaaatattt cttttgaagt aaatgatgga gagttagttg gattaattgg
      601 gcttaatggt gcaggaaaat caaccactat taacgagatt attggtattt tgagacccta
      661 tcaaggagat attaccattg atggcattag tcttgaagcc aaccaagaac tttatagaaa
      721 gaaaataggc tttattcctg agacaccaag tctctatgag gaactaactc tcagagaaca
      781 tttagaaacg gtagctatgg cgtatgatat tgccaccgat gaagttatgg cacgtgcgca
      841 aaaactatta gagatgtttc gtctaacaga taaattagac tggtttccaa tgcatttttc
      901 aaagggaatg aaacaaaagg ttatgattat ctgtgctttt gttgtatctc caagtttatt
      961 tattgttgat gaaccatttt taggtttgga tccactagct atttcagatt tgattaatct
     1021 tctagcagaa gaaaaagcta aagggaaatc tattttgatg tcaacacacg tattagattc
     1081 tgccgaaaaa atgtgtgatc gttttgttat tttacataaa ggagaaatac gggcagttgg
     1141 aaccctagag gaattacggg ctatttttgg agacagtaat gctaatttaa atgacatcta
     1201 catagctctg acaaaagagg tgtgatatga aaaagttatt taataaacgt cgaagtctct
     1261 ttttaactca aaatagtaaa tatttgagat acgtctttaa tgatcatttt gtattagtat
     1321 taatgttttt gtcaggtttt ttgttatatc agtatagcca gttactaaaa gactttccaa
     1381 aaacacattg gccaattatc gtgattgtgt caatcattat cttaatgtta ctagcgatgg
     1441 gggaaatagc cagctattta gaaccggctg ataaacaatt tttacttata aaagaagaag
     1501 caataaagga aattataaat tctgctaaaa aacggactta tatcttttgg ttggttgttc
     1561 aaaccttatt tttagtatta atatcaccaa ttttgataaa gttaggcttg tcagttttta
     1621 tgattacatt gttaattttt ggtctaggaa taatcaaatg gttggtgata acttacaagg
     1681 taaaggtctt ttataacaat caaaatttga actgggatgc agctataaat catgagcagg
     1741 aaagaaaaca ggctatatta aaatttttct ctttgtttac aaatgttaag ggaatttcga
     1801 caagtgtaaa acgtcgttca tttttagatg ttattttaaa acttatttct aaaacgccat
     1861 cgcgtttatg gactaactta tttgtgaggg catttctaag aagctcagat tatcttggtt
     1921 taaccataag gttggtaact ttaaatatct tatcagtcat ctttgttaac gaaacctacc
     1981 tcgcccttgc tctggcattt gtatttaact acttattatt gttccaatta ttagctttag
     2041 gtcaccattt tgattatcaa tacatgaatc agctctatcc agttagatta aatgctaaag
     2101 caagtcagct aaaaggtttt ctcagagtgt tatcatatgc tgtgacagta attgatatca
     2161 tacttataag agaattgaaa cctgttatat tattgatagt tttgatgtta attgtgacgg
     2221 aatattacat cccttacaaa ataaagaaga tgattgacta aagcctataa aacaagtaaa
     2281 ataagaggaa acgactaaat gaaaaggagg gactgctggt gacaatatca aatcaagaat
     2341 tgacgcttac tcccttaaga ggaaaaagtg gcaaagccta tattggtact tatccaaatg
     2401 gtgaacgtgt ctttgtcaaa tataatacga cacctatttt gccagcctta gctaaggaac
     2461 agattgcgcc acaactactg tgggcacgcc gtacaagtaa tggcgatatg atgagtgctc
     2521 aagaatggtt agatggtcga acattgacta aagaagatat gggtagtaaa cagattattc
     2581 atattttact gcgcctgcac aagtctcgtc ccttagtaaa tcaactgtta caattagggt
     2641 ataaaattga aaacccttat gatttgttga tggattggga aaaacaaact cctattcaaa
     2701 tccgagaaaa cacttatttg caatctattg tgactgagtt aaagcgtagt ttacctgagt
     2761 ttcgaacaga ggttgcaaca atcgttcacg gagatatcaa acatagcaac tgggttatta
     2821 caacaagtgg tttaatttat ttggttgatt gggattctgt tcgtttaaca gatcgcatgt
     2881 atgacgttgc atatatttta agtcattata ttccacaaaa acactggaaa gattggctgt
     2941 cttactacgg ttataaagat aatgagaaag tctggagcaa aattatttgg tatgggcaat
     3001 tttcatattt atcacagata atcaaatgtt ttgataaacg agatatggag catgtcaatc
     3061 aagaaattta tgaattacga aagtttagag agttaattaa aaagtataat gcgagttaga
     3121 aaacgtaagg gtgcagagga acacttagaa aataatccgc actatgttat ttcaaatcca
     3181 gaagaagcca aagggcgttg gcatgagatt tttggaaata ataaccccat tcacatagaa
     3241 gttggttccg gaaaaggggc ttttatcact ggtatggcag agcaaaatcc agatatcaat
     3301 tatattggaa ttgatattca attgtcagtg ctttcctatg ctctggataa agttctagat
     3361 agcggtgcta agaatatcaa attactatta gttgatggtt ctagtctatc aaactacttt
     3421 gatactggcg aagttgattt gatgtatctt aactttagtg atccatggcc taagaaaaag
     3481 catgagaaac gtcgattgac ctataagact ttcttggata cctataaaga catacttcct
     3541 gagcaggggg aaattcattt taaaacagat aatagaggtt tatttgaata tagccttgct
     3601 agcttttctc aatacggtat gactttaaaa caagtttggt tagatttaca tgcaagtgat
     3661 taccagcaaa atatcatgac tgagtacgaa cgcaaatttt caaataaagg acaggtcatc
     3721 tatcgtgtag aagcacgatt ttagaataag taaatagtaa gtgggtagtt atttgctact
     3781 cctttatgga gaggtcgcat agtggctgag tgcgcacgct tggaaagcgt gtagtcccta
     3841 atgggggctc gggggttcga atcccctcct ctcctttaat cttgttaatg tatcagtgtt
     3901 tattaagtgt tcaccttttt cactcgtcaa aagtccaagt ttcgaaaaat ttcttttatt
     3961 ttatcatcgt ttctctttga gcttgaagta ttagcataaa gttttctaag ctcttacaaa
     4021 gaattgagga caaattttaa ttttttgata taagattagt gtacggtgat gctaatttaa
     4081 agtaaatgat tgcaataatc gtattttgtg ctataatagt attaaataat acagaaaggg
     4141 gcggagtttt acttcgcctc ttatgtgtgt gattcataga atttatagtc attgtaagtt
     4201 ctaaagcaag ttagaaagaa gaggtagctg ttatcgcaaa tcaaaccatt gttgatattg
     4261 ttactcaagt cgtaactcct gctattcaag caccgtttga attagtagat gtagaatacg
     4321 aaaaaatggg tggcgactat gtgctcagta ttttaattga taaacccgga ggcattacgg
     4381 ttgaagatac agcacaattg acagacgttg taagtccctt acttgacaca attcaaccag
     4441 atccatttcc agaacagtat atgcttgaag tctcaagtcc tgggttggaa aggccactta
     4501 aaacagcaga agcattatca aatgctgttg ggtcttatat caatgtaagt ctgtataaat
     4561 ccattgataa agttaaaata tttgaaggtg acttattatc atttgatggt gaaaccttga
     4621 caattgatta tatggacaaa acacgtcata aaactgtaga tattccctat cagacggttg
     4681 caaaagcccg tcttgcggtt aaactttaag aaaggatgat tcttgcgaag caagaatgga
     4741 cactatgagc aaagaaatgc tagaagcctt ccgtattttg gaagaagaaa aacacattaa
     4801 taaagaagat attattgatg cagttactga gtcactaaaa tctgcctata agcgtcgtta
     4861 tggtcaatca gaatcatgcg ttattgaatt taacgagaag aaagcagact ttaccgtcta
     4921 tactgttcgt gaagttgttg atgaagtttt tgatagccgt ttggaaatta gcctaaaaga
     4981 cgcacttgct attagctcag cgtatgaact tggtgataag attcgctttg aggaatctgt
     5041 tacagaattt ggacgcgtgg cagctcagtc agcaaaacaa actattatgg aaaaaatgcg
     5101 tcgtcaaatg cgtgaagtaa ccttcaatga atacaaacaa cacgaaggtg aaattatgac
     5161 tggtactgtt gaacgatttg accaacgttt tatctatgtc aatctcggtt cactcgaggc
     5221 gcaattatcg catcaagatc agattcctgg ggagagcttt aaatctcacg atatgattga
     5281 cgtttatgtc tataaagtgg agaataatcc taaaggtgtc aacgtttttg ttagccgtag
     5341 tcatccagag tttatcaaac gcattatgga gcgagaaatt ccagaagtat ttgatggaac
     5401 tgttgagatt atgagcgtct ctcgtgaggc tggagatcgt actaaagttg ctgtacgcag
     5461 tcataattca aacgttgatg cgattggaac aattgttgga cgcggtggaa gtaacattaa
     5521 aaaagttatc agtaatttcc atcctaaacg cgttgatgct aaaacaggat tggagatccc
     5581 agttgaagaa aacattgatg ttattcaatg ggttgaagat ccagcggaat tcatttacaa
     5641 tgctattgca ccagccgaag ttgatatggt actttttgat gatgaagata caaaacgtgc
     5701 tactgtagtt gttccagata gcaagttatc gctagctatt ggtcgccgag gacaaaatgt
     5761 gcgtttagca gctcatttga ctggctatcg tattgatatt aaatctgcaa gtgaatacga
     5821 aaaaatggaa gcgcaagaac ttcaaactga ggaagtagct caagaatctg aagttatttc
     5881 agataaggca gaataatata aatgagagag gtgttttatg gctaaaacaa aaaaaatacc
     5941 tttaagaaaa tcagttgttt ctggtgaagt gatcgataag cgagatcttt tacgaattgt
     6001 taaaaataaa gaaggacagg tttttataga tcctaccgga aagcaaaatg gtcgaggtgc
     6061 ctacataaaa ttggacaatg atgaagctat cctagcgaaa aagaaacgag tgtttgatcg
     6121 cagtttttca atggaagttt ctgatgaatt ctatgatgaa ctattggcat atgtcgacca
     6181 taaggtcaaa agaagagagt taggccttga ataattcaga aaaagtatta aatctcattg
     6241 ggttagcaca gcgtgcgggg cgccttatta caggtgaaga attggttatt aaagcaattc
     6301 aaaatcaaca agtatcgcta atctttctag caaatgatgc tggcccaaat ttgacgaaaa
     6361 aagtaactga taaaagtaat tactacaaaa cagaagtctc cacagtgttt tcaacactgg
     6421 aattaagtga tgcgttaggc aaaccaagaa aagtggttgc tgtcgcagac gctggatttt
     6481 caaagaaaat gaggactctt atggcataac agaataggag gacatcaatt gtcaaagaaa
     6541 agattacacg aaatcgctaa ggaaattgga aaaacgagta aggaagtggt tgagcaagct
     6601 caatcgcttg gcttacctgt aaaaagtcat gcttctagcg ttgaagaaaa tgatgctaca
     6661 cgtattgttg agagttttag ctcttctaaa actaaagctc caacaaattc tgttcaaact
     6721 aatcaaggtg tgaaaacaga atctaaaact gttgaaacca aacaaggctt gtccgatgat
     6781 aaaccttcaa ctcaacctgt agctaaaccg aaaccacaaa gtcgtaactt taaggcggag
     6841 cgcgaggctc gtgctaaagc tgaagcggag aagcgtcaac ataatgggga tcaccgtaag
     6901 aataatcgcc ataatgatac acgctctgac gacagacgcc atcaaggtca aaagcgttca
     6961 aatggtaatc gtaatgataa ccgacaaggt cagcaaaata accgtaataa aaatgatggg
     7021 cgttatgctg attataaaca gaaaccccaa acgcgtcctc agcaacctgc aggcaatcgc
     7081 attgacttta aagctcgagc agcagctttg aaagctgagc aaaatgctga atactcacgt
     7141 catagtgagc agcgtttccg tgaagagcaa gaagccaaac gtcaagcagc taaggagcaa
     7201 gaactagcta aagcagcagc gttaaaagcg caagaagaag ctcaaaaagc taaggaaaaa
     7261 cttgcttcta aacctgttgc taaggtaaaa gaaattgtca ataaagtagc cgctacgcca
     7321 tcacagacag cagatagtcg tcgtaaaaaa caaactcgtt ctgataagtc tcgtcaattt
     7381 tcaaatgaaa atgaagatgg tcaaaagcaa acaaaaaata agaaaaactg gaataatcaa
     7441 aaccaagtga gaaatcaaag aaatagtaac tggaatcata ataagaaaaa taaaaaaggt
     7501 aagactaatg gagctccaaa accagtaaca gagcgtaaat tccatgaatt accaaaagaa
     7561 tttgaatata ctgagggcat gaccgttgcg gaaattgcaa aacgtattaa acgcgaacca
     7621 gcagaaattg ttaaaaaact ctttatgatg ggagttatgg caacgcagaa tcaatcatta
     7681 gatggagaca ctattgagct attgatggtt gactacggga ttgaagcaca tgctaaggtt
     7741 gaagttgatg aagctgatat tgaacgcttc tttgcagatg aggattacct taatcctgat
     7801 aacttaacgg aacgtccgcc tgttgttact atcatgggac acgttgacca tggtaaaaca
     7861 actcttcttg atactctacg taattcacgt gtcgctactg gcgaagctgg tggaattact
     7921 caacatattg gtgcttatca gattgaagaa gctggcaaga aaattacttt cttggataca
     7981 ccagggcatg cggcctttac ttctatgcgt gcgcgtggtg catcagtaac tgatattact
     8041 atccttatcg ttgccgctga tgatggtgtc atgccgcaga cagttgaggc aattaatcac
     8101 tctaaagctg cgggtgtccc aattattgtt gctatcaaca aaattgataa acctggtgca
     8161 aatccagaac gcgttatcag tgaattagct gagcatggtg tcatctctac tgcatggggt
     8221 ggtgagtctg aatttgttga aatttcagct aagtttggta aaaatattca agaattactt
     8281 gagacagttc ttttagttgc tgaaatggaa gaattaaaag cagatgcaga tgttcgtgct
     8341 attggtactg ttattgaagc ccgtcttgat aaaggtaagg gagccgttgc aacgcttctt
     8401 gtccaacaag gtactttaaa tgtacaagat ccaatcgttg ttggtaatac atttggacgt
     8461 gttcgtgcga tgactaatga tcttgggcgt cgtgtcaaag ttgcgggtcc atctacacca
     8521 gtttctatta ctggtttgaa cgaagctcca atggctggtg atcattttgc tgtttatgca
     8581 gatgagaaag ctgcgcgtgc tgcaggtgaa gaacgtgcaa aacgtgctct cttaaaacaa
     8641 cgccaaaaca cgcaacgtgt tagcctcgag aacttatttg atacccttaa agctggtgaa
     8701 gttaagtcag ttaatgttat tatcaaagct gatgtgcaag gttctgtaga agcacttgca
     8761 gcttctctcc ttaagattga tgttgaagga gtaaaagtta atgttgttca ctcagcagtt
     8821 ggtgctatta atgagtctga tgtaactctt gctgaagcat caaatgctgt tattatagga
     8881 tttaacgtac gccctactcc acaagctcgt caacaagccg atgctgatga tgtcgaaatt
     8941 cgtcaacata gcattatcta taaagttatc gaagaagttg aagaagcgat gaaaggtaaa
     9001 cttgatcctg aataccaaga aaaaatcctt ggtgaagcca ttattcgtga gacctttaaa
     9061 gtttcaaaag tgggaactat tggtggattt atggttatca atggtaaagt tactcgtgat
     9121 tcaagtgttc gtgttatccg tgatggtgtt gtcatttttg atggtaaatt agcaagtctt
     9181 aaacactata aagatgatgt taaagaagtc ggcaatgctc aagaaggtgg tctaatgatt
     9241 gagaattata atgatctcaa agaagatgac actattgaag catacattat ggaagaaatt
     9301 aaacgtaaat aacccctatg agaaacccaa cttgtatctt tagttggtag ggaatacaaa
     9361 attatggcta ggctcgtcct agctttcaag ttatgagcac aatgaagaaa ggattaatta
     9421 tggctaatca tcgtattgat cgcgttggaa tggaaattaa acgtgaagtc aatgagattt
     9481 tgcgtctccg agttaatgat ccacgtgtac aagatgtcac aattactgat gttcagatgt
     9541 tgggtgacct ttcaatggct aaagtatttt atactattca ttcgacactt gcttctgata
     9601 atcaaaaagc acaaattggt ttagaaaaag caactggtac tattaagcgt gaacttggca
     9661 aaaaccttac tatgtataaa ataccagatt tacaatttgt taaagatgaa tctattgaat
     9721 acggaaataa gattgatgaa atgttgcgaa atctagataa aaaagattaa gaataataaa
     9781 aaataaacca tgtcaaaatc atggtttatt ttttatagct ttagagttga gatagctagt
     9841 attaaggagt tttattgatg atatcaatca tttgatactg tgctgtttta gcttcttcaa
     9901 tagggtaaat tggataaaca tgattcattc cctcttgtgc aatatagtta acggggtgat
     9961 tagctgcttg gagttgtgca gcataatcgc gaatatctgg gaagaatatt tctcgtgttc
    10021 ccgtaaataa cgtaatagga gcgagtttag tagcaggggc atttttagga ctaacatatg
    10081 tatggttagt attattagaa ccatttgccc aaatttcacc tacacgtgcg agaccccaag
    10141 cagataggat aggatcggta tcttcgtatt tgggaatttc tggatgtttc attgtcacat
    10201 ctagccatgg agataagagg ataatatttt taggttgagg aatagcctct tgccctgatt
    10261 ggtgagacaa tgcatgcgct aatcctagtg ctaatcctcc gccagctgaa tcacccatca
    10321 aagtgagatt agcgtttttt tcatgaaagt gtctataaag gttaaccaat ctagggatag
    10381 cataatcata ggtataacga ggagtttttg gataaattgg taaaataatt tttgcatcaa
    10441 gactagttga gagcgtctta agcatgctga tatggtaagg cgttggatta tttaagtaag
    10501 agccaccagc taaatagaaa atggtctttt gattagcttt accgttgtca ttccaagaat
    10561 agacttgcat atcaaaaagt ctctcttgaa caagccctcc attaacagag tttgggtcta
    10621 agtaataacc cttatcagat tgttcacggc tagcatttaa gtacttttga aatgagcttt
    10681 catcttgaac tttataataa cttttatcgc gctttagcca aaagtattgt tcgataaacc
    10741 aacttttcca tgaacgccca cttgcttttg catgtggtgt acaggcgatg gataaaatgg
    10801 tgataatact aaatagtagc aatagttttt tgaaggacgg tttcaaggta ttctccttaa
    10861 tataatgtta tttttgtgta atattattat aataaaacca acatctttgt caatagaata
    10921 aagaaagatg agaaatgttt ttcggtttac aaatgtagat ttatttgcta aaattggttt
    10981 acaaacgtaa atcgaaagag gtgagaaaat gactatttca aacgcagaat gggaaattat
    11041 gagagtggtt tgggcacagc agaatacaac cagtaatgag atactagcag ttttactaga
    11101 gaagtatgat tggactcctt caactgtcaa aaccttatta agaaggctct tagataaagg
    11161 ctacgtgagc agagaaaaaa tgggaaaagg atttagctat tcttctttaa ttgatgaaga
    11221 tttagctatg atgtcagagg tagatagtgt attgcagaaa gtttgccaga ccaaacatgt
    11281 tgctattgtt agacacttat tggaaagtat accaatgaca gagaaggatc gattaaattt
    11341 gcaagcatca ctagaagcta agaaaggcaa aacacttgaa cgtgtagctt gtaattgcat
    11401 tcctggtcag tgtcaatgcc attagaaagg agaaaatatg gctaatagaa aagaaacatt
    11461 tttgattgat gggatgactt gtgcatcttg tgctttaacg atagaaaaag ctgtcaacaa
    11521 gttagaccat gtggatagtg cagtagtaaa tttagcaaca gagaaaatga cagttacgtt
    11581 tgatgataca accttatcgc caaacgtgat tgaagagtgt gtttctgaga ctggatacga
    11641 agcatcgctt ttcaaagagg aaacttctaa aagccaatct gaacggcacc aattagcaat
    11701 tgaaaagatg tggcatcgct tttggatgtc agcagttgca actatacctc ttctctatat
    11761 ttccatggag ccaatgatta acttatggtt accatcattt ttgatgccag ataagggacc
    11821 tctaaactat ggtgtgatac aattattgtt aacactaccg gtgatgtatt ttgggcgtat
    11881 cttttaccaa aatggtttta aagcactttt taaaaggcat cccaatatgg attcattagt
    11941 tgctatagcc acaacagcgg ctttcattta tagcttatat ggattgtacg aaatattaca
    12001 gggtgacata cactacgctc accagcttta tttcgaatct gttgcagtta ttttaacact
    12061 tattactcta ggaaaatatt ttgaaatatt atccaaaggg cgtacttcgg cctcaattga
    12121 aaaattatta actttatcag caaaagaagc aagagttatc aaagatggtg aagactatat
    12181 ggttcctttg gataaagtta aaataggtga aacaattctt gtcaaacctg gagaaaaaat
    12241 tcctcttgat ggccatgttg tagcaggaga atcaagtatt gatgaatcca tgttaacagg
    12301 tgaaagtatt ccagttgaaa aaaaggttgg tagtaaggtt tacggtgcca gcattaatgg
    12361 gcaaggtagc ttaacgattt tcgttgaaaa agaagctgtc ggttcccttc tatcacaaat
    12421 tattaactta gtagaagttg cacaggcaag taaggctcca attgctaact tagcagacaa
    12481 agtatcagga gtctttgtcc cttttgttat tgtcattgca attttatcag gtttgagttg
    12541 gtacttaatt ctggggcaaa gttttgcctt ctctctgaag attatgattg cagtcttagt
    12601 gattgcttgt ccatgtgctt tagggttagc aactcctaca gctattatgg tggctagtgg
    12661 taaagcagct gagaatggta ttttatttaa aggtggtgaa gtgttggaaa aggctcacca
    12721 cattgataca atagtttttg ataaaacagg aaccttgacc aaagggaaac cagaagtagt
    12781 tgctatcaaa acatatgggg tagataaaga ggagttttta ggtcaagtag catcagtaga
    12841 aaaactttca aatcatccac ttagccaaac aattgttaac aaagcaaaag agaaagaact
    12901 tcccttaagg gaagtcatgg cttttaaaaa tattttgggt tacggtttga gtgcaattat
    12961 taatggtaaa actatgcttg taggtaatgc taacttaatg actaagaacg atgttaattt
    13021 agatttagca aaggctgata tagaaatagc tcaagaagaa gcacaaactg tggtttacgt
    13081 ttccgaaaat ggagtgttga gcggtttaat aactttaacg gaccagctaa agacagatag
    13141 ccaagaaaca gttaagcagt tacaaagatt aggatttaat ttagttttac ttactggtga
    13201 taataaggct agtgcagatg ccatagcaca aaaattgggt atcacgacag ttgtttcaga
    13261 agttttacct gatcagaaag caaacgttat tttagaactt aaagaaaaag gcggacagat
    13321 tgcgatggtt ggtgatggca tcaatgatgc tcctgcgcta gctagcagtg atgtaggtat
    13381 ctctatgagt tctggaacag atattgctat tgaatcagca gatatcgtgt tgatgaaacc
    13441 tgagttaaca gatctcttaa aagcaatgac tatcagtaaa caaacgattc aaattattaa
    13501 ggaaaattta ttttgggctt tcttttataa cgttttagcg attccagtgg caatgggtgt
    13561 tttacatctt tttggtggtc ccttactcaa tcctatgttg gcaggcttag ctatggcgtt
    13621 tagttcagtt tcagtagttt taaacgcact tcgcttaaaa gtcttaaaat aataaagata
    13681 ataagactga atttttatga agggagaaga atatgaaaca tacctatcga gttagtggca
    13741 tgaaatgtga tggttgtgcc aaaacagttt ctgataagtt atcaagtgtg atgggtgttg
    13801 acgaggttaa tgttgatttg actaaaaatc aagttgtcgt aagtggaaaa acattcaaat
    13861 gtcttttaaa acgttcttta aaagacacaa agtattcact agaagaggaa ataaattaat
    13921 aactaaagaa gtggttacag cttttaattt cttaatttga gcagtttctc acttcttttt
    13981 tattctaaaa atgataaact agaatagaat tagttgtgag gagtgcagat gtcaatcgat
    14041 atttggaaca ttttaagttt ggttggaacc gtagcttttg caagttcggg agctattgtg
    14101 gcgattgagg aagaatttga tgttctaggg ctttttatct taggttttgt aacggcattt
    14161 ggtggtggag ctattagaaa tgtactaatt ggcttgccta ttgaaacact ttggagtcaa
    14221 ggaattgctt tttatgcagc agcagcagct attttgttta ttatgatttt ccctaacttg
    14281 ttgagtggta agggacggga tgccgaagtt gtatcagatg ctattgggtt agcagcgttt
    14341 agtgttcaag gagcactata tgcgactcaa tctcatcaac ctcttagtgc tgtcattgtc
    14401 gcagcagtac taactggtgc aggtggtggt attgtcagag atgttctggc tggccgaaaa
    14461 cctggggtac taagaagtga aatatatgca ggttggtcaa tattagtggg gatcatttta
    14521 tattttaaaa ttgctaaaac aacaactgac tattatttgt tagtgttagt tgtaactagc
    14581 ctaaggatgt tggggtataa gaaacaatgg cacttgccag ttgtcagacc acgtaagaaa
    14641 tgatagagga gaaagagatg ataaaattaa ttgctattga tatggatgga acgcttttaa
    14701 atgatgaaaa gaaaattcca aaggaaaata tccaagctat taaagaagca actcaagcag
    14761 gtattaaaat tgtactgtgt actgggcgac cgatgtcagg tatacttcct tattttaatg
    14821 aactaggttt aacaaaagag gagtatatta tcatgaataa tggttgtagc acttatagca
    14881 cgaaagattg gcaattgatt gattctgcaa ctttaacaca tgatgaactc atctttcttg
    14941 aggaggttgt taaagaattt cccaacgttt gtttaacgtt aacagctgaa aatacatttt
    15001 acgctgttgg tgaggaagtt cctgaaattg ttgcttatga tgctgattta gtttttacaa
    15061 aagcaaagtc aataagttta gatgctcttc gcaatcaaga agaaattgtc tttcaagcaa
    15121 tgtacatggg attagatgca gatgtaactg cttttcaaga agctgttgaa gaagccttaa
    15181 tctcgaaatt tagtggagtt cgaagccaag actatattta tgagattatg cctcaaggag
    15241 taacaaaagc aagaggctta aaatccttga tagcaaaatt aggactggat attaatcaag
    15301 ttatggctat tggtgatgct cctaacgata ttgaattatt agatttagtt cccaatagcg
    15361 ttgctatggg gaatgcatca gatgagatta aatctagatg taaatatata acggtggata
    15421 ataaccaagc cggggttgct aaagctattt atgactatgc tttacgttaa aatagatata
    15481 gaataataaa aggaacttta aacactgttt gtttagggtt cttttgttta ataaactaac
    15541 aaaattcaga agtttacaga tagcttatgc tataatagac ctatgacaaa caagaataaa
    15601 ttacttttaa ttgatggctc atctgtagcc tttcgtgctt tttttgctct ttataatcaa
    15661 attgaccgct ttaaaaacaa ttctggactt catactaatg ccatatatgg tttccatttg
    15721 atgttgaatc atattttagg tcgtgtccag cctagtcata ttttagtggc ttttgatgct
    15781 ggaaaaacta ctttccgtac ggaaatgtat gcagattata aaggtgggcg tgctaaaact
    15841 ccggatgaat ttcgtgaaca atttccttat attcgtcaac aattagatgt cttaggcatc
    15901 aaacattatg aattggaaca ttatgaagcc gatgatatca ttggtactct tgcaaaacaa
    15961 gccgaagcta gtaaagagca ttttgatata acggtggttt ctggggataa agatttaatt
    16021 caattaacag acactaacac ggtagtagaa atctctaaaa agggagttgc tgagtttgaa
    16081 gaattcacgc cagcttacct tatggaaaaa atgggaatta cgccgagtca atttattgat
    16141 ttaaaagctt tgatgggaga taaatcagat aatattcctg gggttactaa aatcggtgaa
    16201 aaaactggct tgaagttgct atcagaatat ggttccttag aagggatcta tgaaaatatt
    16261 gaggctatga aacagtctaa gatgaaagaa aaccttatca atgataagga gcaagctttc
    16321 ttatcaaaga ccctagctac aatcaacata gcatcaccta taacaatcgg cttagaagat
    16381 atcctttata gtggaccgca agatataaaa gccttgtctc aattttatga tgagatggat
    16441 tttaaacagt ttaaagcagc tctgggtgag gagacaagtc aggaagattt tgaggttgac
    16501 tttactgaag tggagcaatt aaaaccagaa atgttctccg ataatgattt ttattacttt
    16561 gagatgttag gtgataacta ccacgtagaa gatttaattg ggatagcttg gggaaatagc
    16621 gatacaatct acgctactag tgatgtatcg ttattgcaag aagctttatt taaaaaagct
    16681 ttatcaaaac ctatcaaaac ttatgatttt aaaagaagta aggtcctttt aaatcgtttt
    16741 aatattgatt taccagagcc agcctttgat actagattgg ccaagtacct tttgtctact
    16801 actgaggata atctagtatc aacaattgct aggttatata caaatctgcc actggatact
    16861 gatgatgctg tttatgggaa aggtgctaaa cgcgctatac cagaaaaaac acgatttctt
    16921 gagcatctag ctaaaaaagt aaaagtttta gtggattctg aggctaatat tatgcaacag
    16981 cttaaagcta atgagcaaga agaattgctg tttgaaatgg agcaaccttt agcgaatgtt
    17041 ttagctaaaa tggaaattcg aggtattaag gttaagaaaa ataccttgaa tgaaatggct
    17101 attgaaaatc aaaaagtcat cgaaacctta acacaagaga tctatgaatt ggctgggcaa
    17161 gagtttaata ttaattctcc aaaacagtta ggaaagttgt tgtttgaaac gttaggtctt
    17221 cctgtagaaa tgactaaaaa aacaaaaaca ggttattcaa ctgcagtaga tgtattagag
    17281 cgcttagctc cgatttctcc tcttgtaact aaaattttag agtaccgtca aatcactaaa
    17341 ttgcaatcaa cctatattat tggtttacaa gattatattt tagaagatgg taaaattcat
    17401 acacgttatg ttcaagattt gactcagaca ggtcgtcttt caagttctga cccaaacctt
    17461 caaaatatac cagttagact agagcaagga cggcttatcc gaaaggcttt tgttccatca
    17521 gaagacaatg ctgttttatt gagttcagat tattctcaaa tagaattgcg cgtgttagca
    17581 catatttcaa aagacgaaca tttgatcgct gcctttaaag agggagcaga tattcatact
    17641 tcaacagcta tgcgtgtttt tggtatcgaa aaacctgaga atgtcactcc caatgatcgt
    17701 cgaaatgcta aggctgttaa ctttggaatt gtttatggta tttcagattt tggtctatcg
    17761 cataatttag gaatacctcg taaactagcg aagcaatata ttgacaccta ttttgagcgt
    17821 tatcctggaa taaaaaacta tatggaaaca gttgttcgtg aagcaaaaga taagggttac
    17881 gttgaaacac ttttccatcg tcgtcgttca ctaccagata ttaattctcg taattttaac
    17941 attcgccaat ttgcagagcg cacagcaatc aattcaccaa tccaaggaag tgcagctgat
    18001 attcttaaaa ttgcaatgat aaatttagat agggtacttg ataaaggtgg ttataaatca
    18061 aaaatgctgt tgcaagttca tgatgaaatt gtacttgaag tccctaacga ggagatagga
    18121 gcaatcaagg agttggttac aaaaacaatg gagtcagcaa ttagtttatc ggtacctttg
    18181 attgcagatg agaacgcagg tgagacttgg tatgaagcga agtagtggac aacatacgta
    18241 aaggagtcat taatatggtt tatcattttc aaaatctaag tgattttatg ttaaaaaatt
    18301 atttaacaaa agcaaagacc atcgcagttg taggattgtc agaccgtcaa gaaactgcag
    18361 cttatcaagt atcaaaaata atgcaagaag caggttatca aattattcca gttaatccta
    18421 aaaatgcagg acagaaaata cttgggcaaa tgacatatgc ctcacttaag gatgtcactg
    18481 aacatattga tattgttaat atttttcgac gtagcgagta tttacctgat attgcaagag
    18541 aatttttaga agtagacgct gatattttct gggctcagct tggacttgaa agtcaagaag
    18601 cagagacaat tctaaagcaa gcaggacaca aacagattgt catgaataaa tgtctgaaag
    18661 ttgaatacca aaaattacac tcagaaacta actaattacc gtgtttaata ggcaaataat
    18721 ataaattatg ctataataac catgctaaat taaagttcgt gctggaaagg agtcctatgg
    18781 acagtcattc acatggtcat agacctctcg atgcttatga aaacgtccta gagcatctcc
    18841 gtgaaaaacg tattcgaatt actgagactc gaaaagctat tatttcctat atggttaata
    18901 gtcgtgagca tccgagtgca gaaaagattt acaatgattt attaccagaa tatcctaaca
    18961 tgagcttggc aacagtttac aataacctta aggtccttgt ggatgagggg tttgtgacag
    19021 agctaaaact ttgcaattat agtacaactt attatgattt tatggggcat cagcacctaa
    19081 acattgcctg tgaagattgt gggaaaatcg ttgattttgt cgatgttgac ttattggata
    19141 tttctagaga agcacaccaa caaacaggtt ttgaagtgac tagagttcag ttagtagctt
    19201 atggtatctg tccggagtgt cagaggaagc gtaaggataa atataaaaaa gattgaaacg
    19261 taattgttca atcttttttt gtttctagac taagagaaaa agtttaagtt ttactaagta
    19321 ataattaaga aataatttat aattgcttaa aaaatgattt attttttgtt ggtatactct
    19381 cattaacaaa aagaaagagg aatcccttat gaaaatatca caatacaata aatggtcaat
    19441 tcgccgattg aaagttggag ctgcatctgt aatgattgct agtggaagca ttgttgcttt
    19501 agggcaatct catattgttt cagcagatga gatgtcccaa cctaaaacaa ccattacagc
    19561 cccaacagct aacacttcta caaatgtaga aagtagtact gataaggcat tatcaaaagt
    19621 tactacgatg gagacaagtt cagaaatgcc taaaatgcaa aatatggcaa aagttgaaaa
    19681 aacgagtgac aaacctatga tggtggctac ctctgttaga aaaatgatgg caacgccgac
    19741 acctgtagcg atgaccaaaa cgactagtgt agatgaggta aagaaatcaa ctgatactgc
    19801 ttttaaacag acagtagacg ttccggctca ttatgttaat gcagcaaaag ggaatggccc
    19861 atttctagca ggtgtaaatc aaacgattcc atatgaagcg tttggtggag acggcatgtt
    19921 aacgcgttta atccttaaag cttcagaggg agcaaaatgg tcagacaatg gtgttgataa
    19981 aaatagtcca ttattaccat tgaaaggctt aactaaaggt aaatatttct atcaagtttc
    20041 tttaaatggt aatacgacag gtaaagaggg gcaagctctc ttagatcaaa ttaaagctaa
    20101 tgataaacat agctaccaag ccacaattag agtttatggt gctaaagatg gtaaagttga
    20161 tttaaaaaac atgattagcc aaaaaatggt aaccattaac attcctcaca ttacaactga
    20221 tatggaagta aaaaattctc taaagatggc ctttaaagaa aaagtagacg tcccagcaaa
    20281 atatgttagt gctgcaaaag ctaaaggtcc atttttagca ggtgttaacg aaacgattcc
    20341 atatgaagcg tttggtggag atggcatgtt aacgcgttta atccttaaag cttcagaggg
    20401 agcaaaatgg tcagacaatg gtgttgataa aaatagtcca ttattaccat tgaaaggctt
    20461 aactaaaggt aaatatttct atcaagtttc tttaaatggt aatacgacag gtaaagaggg
    20521 gcaagctctc ttagatcaaa ttaaagctaa tgataaacat agctaccaag ccacaattag
    20581 agtttatggt gctaaagatg gcaaagttga tatgaatact attttaggac aaaagacagt
    20641 gatgattcat attaatgtcg ctaaaaaaga tatgaatagc acatcaatga tgatgaaaaa
    20701 agataagatg acaatgccta tgaagaaaga aatgacttca tctaaaataa ataccggtat
    20761 gatgatgtct aacaacaaga tgtcagctaa tgtgcaaatg tcatcacaag ccaaatcaaa
    20821 tgataaagct ggtaaaaaaa tgtctatgat gtctaaaaat ttacctaata caggtgaaac
    20881 aaagcaacaa aatgtaggtg tgctaggtat gcttagtcta gcctttgcga caggtctaac
    20941 tgcgctaggc cttaaaaaat ctaaacaaag atagggatag tatttaattc agaacaccaa
    21001 atttgtttaa aattattgag ggttatcgtg gtaaaatagt gttatactag aggtatagca
    21061 ctattttgac tcattaggag agtagaatga cacaaaaatt attattagtt gacgatgaat
    21121 ttgaaattat tgatattaat agacgttatc tagagcaagc aggatatgaa gtgtcagtag
    21181 ctgcggatgg catagaagca ctcaaagagg tagatgagaa tcgttttgac ctaattatta
    21241 gtgatattat gatgcctaaa atggatggct atgattttat tagtgaagtt ctagttcgag
    21301 aacccaacca accttttctc tttataaccg ctaaagtatc tgaaccagat aaaatttact
    21361 ctttgagtat gggtgcagat gactttattt ccaagccttt cagtcctagg gaattagttt
    21421 tacgtgttaa aaatattcta agacgtatct atgggaatca tcaacaatct gaagtattga
    21481 cgattggtga tttggttatc gaccaaaaac aacgtttggt aatggtagac tgcaatacaa
    21541 tttccttaac caataaatca tttgacttgc tttggatatt agcaaatcat ttgaatcgtg
    21601 ttttttcaaa aacagagctt tatgaacgtg tctggggcga agaattttta gatgacacta
    21661 atactttgaa tgttcatatc catgccttac gcaatgacct tgctaaattt agtacagaca
    21721 ataccccaac tatcaaaaca gtttggggat tagggtataa attagaggaa taaaatgaaa
    21781 ttaaaatatt atattgttat tggttacctt atttctatgt taataactgt agcaggtgtt
    21841 ttctttgggc ttaatcatat gctgattgag actcgaggtg tttactacat cctttcagtt
    21901 acaattattg cctgtatcgt aggtggtata gttaatttgt tcttattatc aagtgtattt
    21961 acatctttga aaaagttaaa acaaaaaatg aaggatattt ctcaaagacg ttttgataca
    22021 aaagcacaaa tttgttcacc acaagaattc aaggatttag agacagcctt taaccaaatg
    22081 tcttcagagt tagagtcaac gtttaaatct ctaaacgaga gtgaacgcga aaaaacaatg
    22141 atgattgcac aactttcaca tgatatcaag actccaatta cctctataca atcaacagta
    22201 gaaggtattt tagatggtat tatatctgaa gaagaagtta actattatct caataccatt
    22261 tctcgtcaaa caaaccgtct taaccatcta gttgaagaat taagttttat tacattagag
    22321 actatgtcag acacggctga acctcataaa gaagaaacaa tttatctgga taaactactg
    22381 attgatattt tatctgaatt tcaattggtg tttgaaaaag aaaatcggca agtaatgatt
    22441 gatgttgcgc cagatgtttc gaaattatct agtcaatatg ataaattatc acgaatttta
    22501 ttgaatttga ttagcaatgc agtcaaatat tctgatcctg gaagtccctt gactatcaag
    22561 gcctattcaa atcgtcaagt tatcgttatc gatattattg accaaggcta tgggataaaa
    22621 gatgaagatc ttgcttctat ttttaatcgt ttttatcgtg tagaatcttc acgaaatatg
    22681 aagacaggag gacatggcct aggtctttat attgcgcgac aacttgcaca tcaactgaat
    22741 ggtgatattt tggtcgaaag tcagtatcaa aaaggcagta agttctcttt agtacttaaa
    22801 ttacaaaaat agctaggaat tattcctagc tattttttgt aatgtttcta taaaagatta
    22861 agcgcgcagt agtttggtaa ggaaggagat agatatagac taatccgaaa ctgaagattg
    22921 taaggaaatg ccaaccaata aaacttaagt ctaaaaggaa gagtctaagt ttattaccat
    22981 tcattagttc tcgactagca actagtactt tagatggtcc ctcatagttt ccactttcta
    23041 attgagtgta aaatactaaa gttgagatac tataggagta atattgttga acgagtttta
    23101 taactgcctt agtagtaatt aggctactaa caactattaa aacaggggca aggataaggt
    23161 taccactcaa aagtagccta aaacttgaaa tgatgattag tagaccaaaa gtacttagta
    23221 gataaaagaa agataaaatt agtcctttta gaacaagtac tgagagtaat ttccagaaaa
    23281 tatggcttga aaatacataa gtattgtccg agaatctaac gtttagtttc tgatttttga
    23341 cgacatctaa cattacaaaa ctgactgaaa taataaaaag tgtgtaaaag aaataaactg
    23401 gaagaggtac aaaaaaatca agtagtgtca tattattata ttgataataa aatgatatat
    23461 ataacgtaat aatactaaga agaatcggga gtaaaaaaag ttgattctta ccttgaagat
    23521 tgcttaatat agtttttgcc tcatttttaa tttctgaatt cgtcatatct cctccataca
    23581 taaaagtaca attcatttca ttataacaca ttccttaaga tgagcatgac aagagtacat
    23641 ttttttgata aaatcttaat ggctaaaaaa gaaattaata caaagtatta catttagata
    23701 ttgattagca ctaaagccca tgactgtttt tcatggaagc aaggagaaaa cgatgactga
    23761 tcatccaata aaatatcgat taattaaaca ggaaaaacac acaggagcca gacttggtga
    23821 aataatcaca ccacatggta catttccgac accaatgttt atgcctgttg ggacacaagc
    23881 aactgttaag acacaatctc ctgaagaact aaaagaaatg ggttcaggaa ttattctatc
    23941 aaatacctac catttatggt tacgtccagg tgatgaattg attgctaaag ctggaggttt
    24001 acataaattc atgaactggg atcaggctat tctaacagac agtggaggct ttcaggttta
    24061 ttctttggct gatagtcgaa acatcactga agaaggagta acctttaaaa accatttaaa
    24121 tggtgcgaaa atgtttttat caccagaaaa agctatttca attcaaaata accttggttc
    24181 tgatattatg atgagttttg atgagtgtcc acaattttac cagccttatg attatgtaaa
    24241 aaaatcaatt gagcgaacaa gccgttgggc agaacgtgga ttaaatgccc accgccgtcc
    24301 tcatgatcaa ggactatttg gtattgttca aggggcaggg tttgaggatt tacgtcgtca
    24361 atcagcacga gatttagtat cgatggattt tccaggttat tcaattggag ggcttgcggt
    24421 cggagaaaca catgatgaga tgaatgcagt tcttgatttt actgttccaa tgcttccgaa
    24481 tgataagcct cgttatttga tgggagttgg tgcaccagat agtttaatag acgccgttat
    24541 ccgaggagta gatatgtttg actgcgtctt acctactcgt attgctcgaa atgggacatg
    24601 tatgacaagt caaggtcgtt tagttgttaa gaatgctaaa tttgcagaag attttacgcc
    24661 tcttgatcca aattgtgatt gctatacttg taaaaattat acacgcgctt atatccgtca
    24721 cttattgaaa gcagatgaaa cttttggtat tcgtttgact agttaccata atctttattt
    24781 ccttgtcaat cttatgaaag atgtcagaca agctattatg gatgataatc ttctagagtt
    24841 ccgtcaagat tttatggagc gctatggtta tggcacgaat aaccgtaatt tttaaaaaag
    24901 ttcctggtaa aacagggctt tttttgatct gtttgttaac cgcgttttaa aaaaagttga
    24961 caatagaaaa acttttgtat aatatgttat aggaggaatc atgatgcaag agtactttgg
    25021 tattggtctt gatagtagca gccgctgtta ccactatcat actaaattgg atattgtcgc
    25081 tttaaaatgt gctgtttgtc aaaaatatta tgcttgttat aaatgtcatg atgcactcga
    25141 ggagcattgt tttgtagcaa caaaatctga tgaaatgttt cctgtcttat gcggttcttg
    25201 tcgtcagatg ttgacattaa aagagtataa aactggtttt tgtccttatt gtcgtatgct
    25261 ttttaatcca aattgtcaac gccatgattc tatttatttt tcgaaggagt aatttttaat
    25321 gtttcacaca aaaagcctaa tccagatggc tatgatggct actttattgg tggtacttgg
    25381 tttcattcct gctctaccac ttggtttcat tccggtccct atcgtcttac aaaacctagg
    25441 ggttatgatg gctggtgtta ttttgggcgg aagaaaaggg agtttatcta ttttgctctt
    25501 ctttcttgtc ggtttagtta ttcctgtatt ctcaggttta cgttcgacta tacctgtatt
    25561 gacaggacca acagctggtt atgttattgc ttggttgttt gttcctttgc ttatctcata
    25621 tggtgtaaaa ttagtaaata aaaagaattt tatagctatt ttccttattg tatggctagc
    25681 aggggtcctt tttgtagatg tagcaggagc agtttggctg gcatctt
//
LOCUS       DATROQ010000018        25680 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.018, whole genome shotgun sequence.
ACCESSION   DATROQ010000018 DATROQ010000000
VERSION     DATROQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25680
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.018"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..194)
                     /locus_tag="VBC94_000703"
     CDS             complement(<1..194)
                     /locus_tag="VBC94_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO6978993.1"
                     /translation="MSTFKRYDEEFKQSLVNLYQTGKTQSELCKDYRVSTSALAKWIK
                     QYSQFKLEDNSVLTAKQIQE"
     gene            327..464
                     /locus_tag="VBC94_000704"
     CDS             327..464
                     /locus_tag="VBC94_000704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6978994.1"
                     /translation="MIIARENHQVTHEHVSILLTCVQELIVEVNQTQPLSREFREKYM
                     G"
     gene            474..729
                     /gene="mobC"
                     /locus_tag="VBC94_000705"
                     /pseudo
     CDS             474..729
                     /gene="mobC"
                     /locus_tag="VBC94_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
     gene            complement(704..1199)
                     /locus_tag="VBC94_000706"
                     /pseudo
     CDS             complement(704..1199)
                     /locus_tag="VBC94_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
     gene            complement(1393..2601)
                     /locus_tag="VBC94_000707"
     CDS             complement(1393..2601)
                     /locus_tag="VBC94_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO6978995.1"
                     /translation="MVTFDFKHVQTLHTISQLPISVMSRDKELVQLYGNEDYLLPYYQ
                     FLKHLAIPYNQDITVYEGLFEESFLIFPVCQYLIAIGPFYPYSPDINRQEQLSSRFLE
                     QFSHRSKKEILSYIKLVPCFPTTSIRSLLVSIDAFFQTQFEASCQQVINHLLEESEQI
                     VADPDIVLHLKHTKKNSFQLPTVLNHLNHIIDLVKLGNTQLLKQEINRLPSSSVTSSS
                     IPALRAEKNLTVVYLTKLLELSFEENTDVAKSYALVKHYMALNEEAPDLIDVSRIRCA
                     ALIDFSESLTNKSISDKQQMYNSILHYVDNHLYSKLKVSDIANYLYISDSHLRSVFKK
                     YSDISLQSYILKAKIKEGQLLLQRGVPIGEVAKLLHFYDTTHFLKTFKKYVGISSNEY
                     LTKYRETSCQ"
     gene            2982..4646
                     /locus_tag="VBC94_000708"
     CDS             2982..4646
                     /locus_tag="VBC94_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO6978996.1"
                     /translation="MKLSKKLLFSAAVLTMVAGSTVEPVAQFATGMSIVRAAEVSQER
                     PAKTTVNIYKLQADSYKSEITSNGGIENKDGEVISNYAKLGDNVKGLQGVQFKRYKVK
                     TDISVDELKKLTTVEAADAKVGTILEEGVSLPQKTNAQGLVVDALDSKSNVRYLYVED
                     LKNSPSNITKAYAVPFVLELPVANSTGTGFLSEINIYPKNVVTDEPKTDKDVKKLGQD
                     DAGYTIGEEFKWFLKSTIPANLGDYEKFEITDKFADGLTYKSVGKIKIGSKTLNRDEH
                     YTIDEPTVDNQNTLKITFKPEKFKEIAELLKGMTLVKNQDALDKATANTDDAAFLEIP
                     VASTINEKAVLGKAIENTFELQYDHTPDKADNPKPSNPPRKPEVHTGGKRFVKKDSTE
                     TQTLGGAEFDLLASDGTAVKWTDALIKANTNKNYIAGEAVTGQPIKLKSHTDGTFEIK
                     GLAYAVDANAEGTAVTYKLKETKAPEGYVIPDKEIEFTVSQTSYNTKPTDITVDSADA
                     TPDTIKNNKRPSIPNTGGIGTAIFVAIGAAVMAFAVKGMKRRTKDN"
     gene            4735..5658
                     /locus_tag="VBC94_000709"
     CDS             4735..5658
                     /locus_tag="VBC94_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6978997.1"
                     /translation="MKQTLKLMFSFLLMLGTMFGISQTVLAQETHQLTIVHLEARDID
                     RPNPQLEIAPKEGTPIEGVLYQLYQLKSTEDGDLLAHWNSLTITELKKQAQQVFEATT
                     NQQGKATFNQLPDGIYYGLAVKAGEKNRSVSAFLVDLSEDKVIYPKIIWSTGELDLLK
                     VGVDGDTKKPLAGVVFELYEKNGRTPIRVKNGVHSQDIDAAKHLETDSSGHIRISGLI
                     HGDYVLKEIETQSGYQIGQAETAVTIEKSKTVTVTIENKKVPTPKVPSRGGLIPKTGE
                     QQAMALVIIGGILIALALRLLSKHRKHQNKD"
     gene            5660..6577
                     /gene="srtC1"
                     /locus_tag="VBC94_000710"
     CDS             5660..6577
                     /gene="srtC1"
                     /locus_tag="VBC94_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC1"
                     /protein_id="HEO6978998.1"
                     /translation="MGQKSKISLATNIRIWIFRLIFLAGFLVLAFPIVSQVMYFQASH
                     ANINAFKEAVTKIDRVEINRRLELAYAYNASIAGAKTNGEYPALKDPYSAEQKQAGVV
                     EYARMLEVKEQIGHVIIPRINQDIPIYAGSAEENLQRGVGHLEGTSLPVGGESTHAVL
                     TAHRGLPTAKLFTNLDKVTVGDRFYIEHIGGKIAYQVDQIKVIAPDQLEDLYVIQGED
                     HVTLLTCTPYMINSHRLLVRGKRIPYVEKTVQKDSKTFRQQQYLTYAMWVVVGLILLS
                     LLIWFKKTKQKKRRKNEKAASQNSHNNSK"
     gene            6534..7385
                     /gene="srtC2"
                     /locus_tag="VBC94_000711"
     CDS             6534..7385
                     /gene="srtC2"
                     /locus_tag="VBC94_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC2"
                     /protein_id="HEO6978999.1"
                     /translation="MKKRLVKIVTIIRNNKIRTLIFVMGSLILLFPIVSQVSYYLASH
                     QNINQFKREVAKIDTNTVERRIALANAYNETLSRNPLLIDPFTSKQKEGLREYARMLE
                     VHEQIGHVAIPSIGVDIPIYAGTSETVLQKGSGHLEGTSLPVGGLSTHSVLTAHRGLP
                     TARLFTDLNKVKKGQIFYVTNIKETLAYKVVSIKVVDPTALSEVKIVNGKDYITLLTC
                     TPYMINSHRLLVKGERIPYDSTEAEKHKEQTVQDYRLSLVLKILLVLLIGLFIVIMMR
                     RWMQHRQ"
     gene            7467..10139
                     /locus_tag="VBC94_000712"
     CDS             7467..10139
                     /locus_tag="VBC94_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEO6979000.1"
                     /translation="MKKRQKIWRGLSVTLLILSQIPFGILVQGETQDTNQALGKVIVK
                     KTGDNATPLGKATFVLKNDNDKSETSHETVEGSGEATFENIKPGDYTLREETAPIGYK
                     KTDKTWKVKVADNGATIIEGMDADKAEKRKEVLNAQYPKSAIYEDTKENYPLVNVEGS
                     KVGEQYKALNPINGKDGRREIAEGWLSKKITGVNDLDKNKYKIELTVEGKTTVETKEL
                     NQPLDVVVLLDNSNSMNNERANNSQRALKAGEAVEKLIDKITSNKDNRVALVTYASTI
                     FDGTEATVSKGVADQNGKALNDSVSWDYHKTTFTATTHNYSYLNLTNDANEVNILKSR
                     IPKEAEHINGDRTLYQFGATFTQKALMKANEILETQSSNARKKLIFHVTDGVPTMSYA
                     INFNPYISTSYQNQFNSFLNKIPDRSGILQEDFIINGDDYQIVKGDGESFKLFSDRKV
                     PVTGGTTQAAYRVPQNQLSVMSNEGYAINSGYIYLYWRDYNWVYPFDPKTKKVSATKQ
                     IKTHGEPTTLYFNGNIRPKGYDIFTVGIGVNGDPGATPLEAEKFMQSISSKTENYTNV
                     DDTNKIYDELNKYFKTIVEEKHSIVDGNVTDPMGEMIEFQLKNGQSFTHDDYVLVGND
                     GSQLKNGVALGGPNSDGGILKDVTVTYDKTSQTIKINHLNLGSGQKVVLTYDVRLKDN
                     YISNKFYNTNNRTTLSPKSEKEPNTIRDFPIPKIRDVREFPVLTISNQKKMGEVEFIK
                     VNKDKHSESLLGAKFQLQIEKDFSGYKQFVPEGSDVTTKNDGKIYFKALQDGNYKLYE
                     ISSPDGYIEVKTKPVVTFTIQNGEVTNLKADPNANKNQIGYLEGNGKHLITNTPKRPP
                     GVFPKTGGIGTIVYILVGSTFMILTICSFRRKQL"
     gene            10193..10983
                     /locus_tag="VBC94_000713"
                     /pseudo
     CDS             10193..10983
                     /locus_tag="VBC94_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            11232..11837
                     /locus_tag="VBC94_000714"
     CDS             11232..11837
                     /locus_tag="VBC94_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEO6979001.1"
                     /translation="MARDPYSDRSRYLDPKVLSSSFGAFFPADNIKVAWSNNSSTLFT
                     PPINANYTTQIQAIGTTIKSQIPESILTVTDKKSRKFSINKIDEAKEGLVGATFTLSK
                     RTTVAADHQVQGDFIPVSKETTVGRTTLTFDNLKPGFYDLKETKAPNAYVLDPKTYVV
                     VVQNSGKTTIVDEANFKEADYPMADNTSHVECVALLQRSKG"
     gene            12358..12548
                     /locus_tag="VBC94_000715"
                     /pseudo
     CDS             12358..12548
                     /locus_tag="VBC94_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883557.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
     gene            12935..13111
                     /locus_tag="VBC94_000716"
     CDS             12935..13111
                     /locus_tag="VBC94_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979002.1"
                     /translation="MHVGKSYFDYQENALIPQLGFLYSKLTKKIELDNRLSPTEQKLL
                     ITLLLHTKGLIIDM"
     gene            14456..14725
                     /locus_tag="VBC94_000717"
     CDS             14456..14725
                     /locus_tag="VBC94_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979003.1"
                     /translation="MVSLLLLKVKQEELIEFHNVFYREAISSYLETENFEKAKVTEEN
                     YQVFLGIRTYQKSYNYQDHSENLKGNIEVAMIGVENNISDEHIFC"
     gene            14786..15937
                     /locus_tag="VBC94_000718"
     CDS             14786..15937
                     /locus_tag="VBC94_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6979004.1"
                     /translation="MVRKFLKSLLSLFLIAVIATGISVACFFFIPENKGNITPILLHR
                     FMRKNNVNGMMIVSDNTGKPITISHGINRGEVETDIENNKLFPMASLQKLMTGIIIQR
                     LIDQDVLSEDDRLSQFFPQVKGSNSITIHQLLTHTSGLREKGVKVSPYLKNEREQLQF
                     CLKHYNFVNKKSWYYSNINFSFLTGIATQVTGRTYAELVDDVIKNPLRLDDTQSYQSV
                     VNHDLVSPMRKNGKLNKINIFNQVSTAYGAGDFFTTPLNFWVLMRSFSKGYFFPTDEY
                     TKHQNDAISHYYGGLYMHGRIVNSNGAFFQYSSFGYADLKTHDTMVLFMNNKVYSDAS
                     HVAPKAFEYYQKFMFLNKIFHLVFYFVFSFFLMLLIRHSFRKRRYKKRL"
     gene            16137..17129
                     /locus_tag="VBC94_000719"
     CDS             16137..17129
                     /locus_tag="VBC94_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6979005.1"
                     /translation="MSMIEVSHLQKNFIKTVKAPGLKGAFQSFLRPEKHTFEAVKDLT
                     FDVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRIDGKLPQENRQNYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDKEFRKRMAFLNEVLELNDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCHRIFMIDRGQEIFDGTVSQLKETFGKMKTLSFDLRPGQEHISSSLIGKSEIN
                     IKRNDLVLDIQYDSSRYQTADIIQQTLADFSVCDLKMTDADIEDIIRRFYRNEL"
     gene            17132..17950
                     /locus_tag="VBC94_000720"
     CDS             17132..17950
                     /locus_tag="VBC94_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEO6979006.1"
                     /translation="MNRIWRRYKPFISTGIQGLITYRVDFILYRIGDVIGAFVAFYLW
                     KAVFDSSSQSLIQGFQLSDMILYIIMSFVTNLLTRTDSSFMIGDEVKDGSIIMRLLRP
                     VHFAASYLFMEIGSRWLIFLSIGVPFLLVITGVRLFLGTDLIQAIVLVVFYIISIILA
                     FLINFFFNICFGFSAFVFKNLWGSNLLKNSLVAFMSGSLIPLTFFPKIVADILGFLPF
                     SSLIYTPVMIIIGKYDGSQIVQALLLQIFWLIVMVALSQLIWKKVQSHITIQGG"
     gene            17952..18737
                     /locus_tag="VBC94_000721"
     CDS             17952..18737
                     /locus_tag="VBC94_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6979007.1"
                     /translation="MTKYQRMHFIFIKQYMKQIMEYKIDFFVGVLGVFLTQGLNLLFL
                     NVLFQRIPSLEGWTFQQIAFIYGFSLLPKGIDHLFFDNLWALGQRLIRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGFILLSTTVSSISWIVPKVLLFIFIIPFATLIYTS
                     LKIATSSIAFWTKQSGAVIYIFYMFNDFAKYPVAIYNNLLRWIISFVIPFAFTAYYPA
                     AYFLQDRNVYFNIGGVILISLISFMVSLILWHKGVEVYESAGS"
     gene            19362..19667
                     /locus_tag="VBC94_000722"
     CDS             19362..19667
                     /locus_tag="VBC94_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylX protein"
                     /protein_id="HEO6979008.1"
                     /translation="MGRSNILSRCEQCSDNQCGLYKTRLKDKLLLVVQLVGNIRYSEF
                     LTEGGVYHKGETLLEVITLGISYEYKSYGDIKVIKKYCADGSVVDYNMPILLMERIE"
     gene            19667..20515
                     /locus_tag="VBC94_000723"
     CDS             19667..20515
                     /locus_tag="VBC94_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylD"
                     /protein_id="HEO6979009.1"
                     /translation="MKTVIIYGGQGNVQSKHLCQLVKTPKGEILFSKLQATDLESYQL
                     MMDVINHKVSLDNVHASMLANFLYNDWLTNDEKIVSGIGFSAHSAGIFNVLYASGSTS
                     FENIIHFIKRRAQLIENCHVSEELWLLMVENIDMFSKDVLKEQSEKVKLAIITDDRAG
                     VIAMDQVNRSFFDDVMTNKGYFYKLKQLGVKAPYHTDFLNESKEQYKQLIESLDISQN
                     DDYDYIFHCDQLVDEILYQWTNLFNWRAIKETIIEKDAEIIDISPNRFISSQLQKMKK
                     RGRKQL"
     gene            20512..21234
                     /gene="fabG"
                     /locus_tag="VBC94_000724"
     CDS             20512..21234
                     /gene="fabG"
                     /locus_tag="VBC94_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEO6979010.1"
                     /translation="MKVAIVTGGTRGIGRAITKELYKEGYKVIAIYNSNDAKARALQE
                     ELPKLDVYKCNISDAKAVQKLVTKIFREYGGIDCLVNNAGIVRDGFFLMMSKEKWMDV
                     ININIMGLVNMSKAVLKIMKAKRIQGKVINISSTSGIAGQIGQANYSATKGAIISITK
                     TLAKEFASDGITINCVSPGFIETDMTNELQNKEELKEHLIPLKRFGQPEEVAWLVSFL
                     ASEKANYITGKNIVIDGGMIND"
     gene            21227..21532
                     /locus_tag="VBC94_000725"
     CDS             21227..21532
                     /locus_tag="VBC94_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6979011.1"
                     /translation="MINWAEERKIIKTRQEVVDEIKGILIDSLMLDIDKDLIMNDQPL
                     FGRGLELDSIDALELSIGISTTFGVELNDDDISILSSVNRLADFVIENSEDIDDQAK"
     gene            21516..21992
                     /gene="fabZ"
                     /locus_tag="VBC94_000726"
     CDS             21516..21992
                     /gene="fabZ"
                     /locus_tag="VBC94_000726"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO6979012.1"
                     /translation="MTKLSSEEIIKMLPHRYPFLMVDRVQSYKKGIIECRKNITINEP
                     YFQGHFPQRPIVPGVLMIEMAAQSAALLYILDALDGSLPSLDELTAEKIAEKVGYLAS
                     VKNFKFKKIVTPGDQLNITCKSQSKLGHLLEIQVVIRDENKKEVASGRMLVSENGN"
     gene            21982..22911
                     /locus_tag="VBC94_000727"
     CDS             21982..22911
                     /locus_tag="VBC94_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979013.1"
                     /translation="MEIKLKNIGKKYGSFEALRDINLIFEEGKFYGLLGPNGAGKTTL
                     FNLLIQNFKQTSGDIKWEVDGKPLSIKDFYRHIGIVFQSNRLDDNLTVEENLISRGAL
                     YGLSKSQVRNRLKDLQTYLDITAIKKQKYGSLSGGQKRKVDIARALLPQPSLLLLDEP
                     TTGLDPQSRRDLWDAIAQLNQQSQMTVVLITHYLEEMSACDVLNVLIEGNIYYSGDIK
                     SFIEQHSTTNLNVVLKPEKSLDQLSIADFVNKCQVLSEREIVFKDISVEEMMQVISDN
                     QDKSIIETFNVEYSNLEAAYLNLLREKERKNNV"
     gene            22904..23782
                     /locus_tag="VBC94_000728"
     CDS             22904..23782
                     /locus_tag="VBC94_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6979014.1"
                     /translation="MFELISRNRKVYTRDRLAFFMSFLSVIILILVYQIFLGKIQLDA
                     IKAALRSNTVSSDTIHMVNYWLIAGLTTIISMTSTLGAFGVMVSDKEKKLNEDFKVSP
                     ISNIKIELSYAIFAVLFGIVMTMFSCIFAIGIFNGFNSLLEYSAMDYLTILGIVSLGT
                     VLSAAMILPILAFIHTSSAFTTLSTIVGTFIGFISGVYLSIGSVGKALQQVMTWFPLT
                     QINSLLKQVLMKGSIAKVFDKANEATVSNYKESYGVVLRNADGERLSNHFMLIYIIAL
                     ILILLAIHFIIKKVKK"
     gene            23779..>25680
                     /locus_tag="VBC94_000729"
     CDS             23779..>25680
                     /locus_tag="VBC94_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylE protein"
                     /protein_id="HEO6979015.1"
                     /translation="MKDDNKLKISEASLEDYSEVVHLFNRNHVYQFPDGRPLTVDDLD
                     LTLKVKEVTHLFLLKNHGVLIGTSAFFKFITYGCLDWNSSFSGFLLIDSKSRSGQAIT
                     YLYKTILKKITKLKFSNIYTEISNYNKPSLALSKLNGFKEYDKTYEDILHCRSLRSHL
                     PKILNTFRISNYYGKTYDISTFQIMEEIENPLEEETEIRTKVSDEEILFKAEDSASLP
                     YYLKMSLFQMEIAKLDNRYVLQVDFLSEQVKRVRVKTGKYHLANLTRAHPSLTLSRFA
                     NYYYIQATVETLYGNIDVQLERRKKHYRDATICLKRTFQGYDLLISPNGSLIFEKQKR
                     KILEDSFLIFSQPLDKKLVVKEEENHITIKCFYQGALIEKIMTFTSDEEITCVYKCNQ
                     KAKEMFPKLLKQTFKLHCQEQLIRDSEGYLVNVPGSYPIEHDDFLRADKFEDRQFHYY
                     LPNEDKMISYSPPGKASNQMQFRPLCLIDTDSLSFPLTYHFRISQASSEEALINLKKQ
                     PIWDSNYQASATDLLKHISNLTLEEEKDYGIKRMIANRKHYPSHKLVLAYNQIVLPKN
                     EIPRDSELYSISFDYRIRGKFVQIRQGEHVKYDNKSYVLENSQQLVLYVASDDKYILI
                     SAKNGIFYSY"
BASE COUNT         8662 a         3922 c         5008 g         8088 t
ORIGIN      
        1 aactcttgta tttgcttggc agtaagtaca gaattatctt cgagtttgaa ttgagagtac
       61 tgtttgatcc attttgcaag cgcagaagtg gataccctat agtctttaca gagttcagac
      121 tgagtttttc cagtttgata aaggttaaca agagattgtt tgaattcttc atcatagcgt
      181 ttaaatgttg acataggttg tcctttcgtt tttgtgtctt ttaaaacaga ttgtaacaca
      241 caaagttttg tctactttta tagtatacat ccaccctttg gcaataccaa aagtttgagc
      301 agattagttg agatgtgcag gaagttttga ttatcgcaag agagaatcat caagtgactc
      361 atgaacacgt gtcaatctta ttgacctgcg ttcaagaatt gattgtggaa gtcaatcaaa
      421 cacagccact cagtcgtgag tttcgtgaaa agtatatggg ttaggaggct gcaattgttt
      481 atcgttatcg taccaatctc aaaaaagtct ttctaacaga ttcagaattg aatcaattaa
      541 atgagcggat tgctaagagc aattgtcaaa atttctcagt ctatgctaga agagttcttc
      601 ttaatcccaa tatgtcattt gtcacgatta acacggattg gaaataacat caatcaaatt
      661 gcgcgtgcta tcaaccaaag ccatctaatt tctcaggctc agtatcactt tcatcgagaa
      721 gcacgttaag cccaacagca gggggggttg ctctgattcg gttaagtaat aagcaaaatc
      781 ctcaccaatt ttcccagtaa taagaggcgt tgttgaggta tagggattac ctgtgccata
      841 gtcgatgatg gtcacaaaat gaccattccc cataaaaggg ccaaccaaaa cttcacctgt
      901 ggccgttttt tgatgtcaac accagtattt tggatgtacc ccttaacatg ccctttggta
      961 tctgcaacag agataatatg accaaaggaa gaatcaccga tgactttaac cgtaatcttg
     1021 ctatcgcctt tttgattagc cgctaatatt tgattagcaa tcagggtgcg accaagagca
     1081 actgttgaag acgacaaggt atgatgtttt tcctgagcca atttaacagt ctcggtgcta
     1141 tcgaggacat aagacctgaa ggcaccagag gcagaaattg atttaataat tttatccata
     1201 acctatgtta tagcacaaag agagagtttg tatggactgg attagctgaa aattttagac
     1261 taaaaagtag tgccagtgga ttttgaaagg taacaaaaga gtcttccgtt taaacgagaa
     1321 ggctgttttt agtcgtttca gatgaaggca aaaggacgat gaaataattc cgtaccttca
     1381 tggattgcta tgttactggc atgaggtctc acgatattta gtaagatatt cgtttgaaga
     1441 tattcccacg tattttttaa aggttttaag aaaatgtgtg gtgtcgtaaa aatgtaataa
     1501 tttcgctact tccccaatcg gtacccctct ttgcagtagt aattgtccct ccttaatttt
     1561 tgcctttaga atataacttt gcaaggaaat gtcagagtat tttttaaaaa ctgagcgtaa
     1621 gtgggaatct gagatatata ggtagttggc aatatcagat actttgagtt tggagtagag
     1681 gtggttgtcc acataatgga gaatactatt gtacatttgc tgcttgtcag agatgctctt
     1741 attggttaag gattctgaaa aatcaataag agctgcacag cgaattcttg aaacatcaat
     1801 aagatcagga gcctcttcgt ttaaagccat atagtgcttt accagcgcat aacttttagc
     1861 cacatcagta ttttcctcga aacttaattc tagtaatttt gttaagtaaa caacagttaa
     1921 gttcttttca gctcttaggg cagggattga agatgaggta acactggatg atgggaggcg
     1981 attaatttct tgctttaaca gttgagtgtt acccagctta acgagatcaa taatgtgatt
     2041 gagatggttt aaaacagtgg gtaactgaaa agagtttttc ttagtatgtt ttaggtgaag
     2101 aacaatatca ggatccgcaa caatctgttc tgactcttct aataaatgat tgatgacttg
     2161 ttggcaacta gcctcaaact gtgtttggaa aaaggcatcg atagacacaa gaagactacg
     2221 tatactggta gtaggaaaac aagggacaag ctttatatag gataagattt cttttttact
     2281 acgatgagaa aattgttcta gaaagcgact ggataactgt tcttgcctat tgatatcagg
     2341 gctataggga taaaatggtc caatagcaat aagatattga cagacaggaa aaattaagaa
     2401 tgattcttca aaaagaccct cataaacagt gatatcttgg ttataaggga tagctaaatg
     2461 ttttaaaaac tgatagtaag gcaacagata gtcttcgtta ccatataact gaacgagttc
     2521 cttgtctcgt gacatgactg aaataggtag ttgagatatg gtatgcaatg tttgaacatg
     2581 tttaaaatcg aatgtaacca tttgatagac cgccttcatt atcatttcta gaatttttct
     2641 ttaggtttgt aaagactaca aaataaaatg atgaaaacaa ctatcttgtg gatacactaa
     2701 aaagacacgc taattagcaa actctctctt catcatctct caccattatt atactactat
     2761 ttatatgaca aataaaggtg attttgttaa aatataactt tgaaaatcca catatatttt
     2821 taatcttccg tctgaaaaaa taaataaaaa tagtaaaaat aaacacgaat ttaaaataag
     2881 caaatttttt aagaaaatct gtgctaaact ttaatagttt tgtgcttaat aataatcagc
     2941 acttacaaag aacaaaggga aaagcgagga gagaactttt aatgaaatta tcgaagaagt
     3001 tattgttttc ggctgctgtt ttaacaatgg tggcggggtc aactgttgaa ccagtagctc
     3061 agtttgcgac tggaatgagt attgtaagag ctgcagaagt gtcacaagaa cgcccagcga
     3121 aaacaacagt aaatatctat aaattacaag ctgatagtta taaatcggaa attacttcta
     3181 atggtggtat cgagaataaa gacggcgaag taatatctaa ctatgctaaa cttggtgaca
     3241 atgtaaaagg tttgcaaggt gtacagttta aacgttataa agtcaagacg gatatttctg
     3301 ttgatgaatt gaaaaaattg acaacagttg aagcagcaga tgcaaaagtt ggaacgattc
     3361 ttgaagaagg tgtcagtcta cctcaaaaaa ctaatgctca aggtttggtc gtcgatgctc
     3421 tggattcaaa aagtaatgtg agatacttgt atgtagaaga tttaaagaat tcaccttcaa
     3481 acattaccaa agcttatgct gtaccgtttg tgttggaatt accagttgct aactctacag
     3541 gtacaggttt cctttctgaa attaatattt accctaaaaa cgttgtaact gatgaaccaa
     3601 aaacagataa agatgttaaa aaattaggtc aggacgatgc aggttatacg attggtgaag
     3661 aattcaaatg gttcttgaaa tctacaatcc ctgccaattt aggtgactat gaaaaatttg
     3721 aaattactga taaatttgca gatggcttga cttataaatc tgttggaaaa atcaagattg
     3781 gttcgaaaac actgaataga gatgagcact acactattga tgaaccaaca gttgataacc
     3841 aaaatacatt aaaaattacg tttaaaccag agaaatttaa agaaattgct gagctactta
     3901 aaggaatgac ccttgttaaa aatcaagatg ctcttgataa agctactgca aatacagatg
     3961 atgcggcatt tttggaaatt ccagttgcat caactattaa tgaaaaagca gttttaggaa
     4021 aagcaattga aaatactttt gaacttcaat atgaccatac tcctgataaa gctgacaatc
     4081 caaaaccatc taatcctcca agaaaaccag aagttcatac tggtgggaaa cgatttgtaa
     4141 agaaagactc aacagaaaca caaacactag gtggtgctga gtttgatttg ttggcttctg
     4201 atgggacagc agtaaaatgg acagatgctc ttattaaagc gaatactaat aaaaactata
     4261 ttgctggaga agctgttact gggcaaccaa tcaaattgaa atcacataca gacggtacgt
     4321 ttgagattaa aggtttggct tatgcagttg atgcgaatgc agagggtaca gcagtaactt
     4381 acaaattaaa agaaacaaaa gcaccagaag gttatgtaat ccctgataaa gaaatcgagt
     4441 ttacagtatc acaaacatct tataatacaa aaccaactga catcacggtt gatagtgctg
     4501 atgcaacacc tgatacaatt aaaaacaaca aacgtccttc aatccctaat actggtggta
     4561 ttggtacggc tatctttgtc gctatcggtg ctgcggtgat ggcttttgct gttaagggga
     4621 tgaagcgtcg tacaaaagat aactaaataa aaggctactt cttaagtaac catgtttaag
     4681 aaaagagaaa tagccttatt tctctttttg tcgtttttaa aataaaggaa catcatgaaa
     4741 caaacattaa aacttatgtt ttcttttctg ttgatgttag ggactatgtt tggaattagc
     4801 caaactgttt tagcgcaaga aactcatcag ttgacgattg ttcatcttga agcaagggat
     4861 attgatcgtc caaatccaca gttggagatt gcccctaaag aagggactcc aattgaagga
     4921 gtactctatc agttgtacca attaaaatca actgaagatg gcgatttgtt ggcacattgg
     4981 aattccctaa ctatcacaga attgaaaaaa caggcgcagc aggtttttga agccactact
     5041 aatcaacaag gaaaggctac atttaaccaa ctaccagatg gaatttatta tggtctggcg
     5101 gttaaagccg gtgaaaaaaa tcgtagtgtc tcagctttct tggttgactt gtctgaggat
     5161 aaagtgattt atcctaaaat catctggtcc acaggtgagt tggacttgct taaagttggt
     5221 gtggatggtg ataccaaaaa accactagca ggcgttgtct ttgaacttta tgaaaagaat
     5281 ggtaggactc ctattcgtgt gaaaaatggg gtgcattctc aagatattga cgctgcaaaa
     5341 catttagaaa cagattcatc agggcatatc agaatttccg ggctcatcca tggggactat
     5401 gtcttaaaag aaatcgagac acagtcagga tatcagatcg gacaggcaga gactgctgtg
     5461 actattgaaa aatcaaaaac agtaacagta acgattgaaa ataaaaaagt tccgacacct
     5521 aaagtgccat ctcgaggagg tcttattccc aaaacaggtg agcaacaggc aatggcactt
     5581 gtaattattg gtggtatttt aattgcttta gccttacgat tactatcaaa acatcggaaa
     5641 catcaaaata aggattagca tgggacaaaa atcaaaaata tctctagcta cgaatattcg
     5701 tatatggatt tttcgtttaa ttttcttagc gggtttcctt gttttggcat ttcccatcgt
     5761 tagtcaggtc atgtactttc aagcctctca cgccaatatt aatgctttta aagaagctgt
     5821 taccaagatt gaccgggtgg agattaatcg gcgtttagaa cttgcttatg cttataacgc
     5881 cagtatagca ggtgccaaaa ctaatggcga atatccagcg cttaaagacc cctactctgc
     5941 tgaacaaaag caggcagggg tcgttgagta cgcccgcatg cttgaagtca aagaacaaat
     6001 aggtcatgtg attattccaa gaattaatca ggatatccct atttacgctg gctctgctga
     6061 agaaaatctt cagaggggcg ttggacattt agaggggacc agtcttccag tcggtggtga
     6121 gtcaactcat gccgttctaa ctgcccatcg agggctacca acggccaagc tatttaccaa
     6181 tttagacaag gtaacagtag gtgaccgttt ttacattgaa cacatcggcg gaaagattgc
     6241 ttatcaggta gaccaaatca aagttatcgc ccctgatcag ttagaggatt tgtacgtgat
     6301 tcaaggagaa gatcacgtca ccctattaac ttgcacacct tatatgataa atagtcatcg
     6361 cctcctcgtt cgaggcaagc gaattcctta tgtggaaaaa acagtgcaga aagattcaaa
     6421 gaccttcagg caacaacaat acctaaccta tgctatgtgg gtagtcgttg gacttatctt
     6481 gctgtcgctt ctcatttggt ttaaaaagac gaaacagaaa aagcggagaa agaatgaaaa
     6541 agcggctagt caaaatagtc acaataattc gaaataataa aattagaacc ctcatttttg
     6601 tgatgggaag tctgattctc ttatttccga ttgtgagcca ggtaagttac taccttgctt
     6661 cgcatcaaaa tattaatcaa tttaagcggg aagtcgctaa gattgatact aatacggttg
     6721 aacgacgcat cgctttagct aatgcttaca atgagacgtt atcaaggaat cccttgctta
     6781 tagacccttt taccagtaag caaaaagaag gtttgagaga gtatgctcgt atgcttgaag
     6841 ttcatgagca aataggtcat gtggcaatcc caagtattgg ggttgatatt ccaatttatg
     6901 ctggaacatc cgaaactgtg cttcagaaag gtagtgggca tttggaggga accagtcttc
     6961 cagtgggagg tttgtcaacc cattcagtac taactgccca ccgtggcttg ccaacagcta
     7021 ggctatttac cgacttaaat aaagttaaaa aaggccagat tttctatgtg acgaacatca
     7081 aggaaacact tgcctacaaa gtcgtgtcta tcaaagttgt ggatccaaca gctttaagtg
     7141 aggttaagat tgtcaatggt aaggattata taaccttgct gacttgcaca ccttacatga
     7201 tcaatagtca tcgtctcttg gtaaaaggag agcgtattcc ttatgattct accgaggcgg
     7261 aaaagcacaa agaacaaacc gtacaagatt atcgtttgtc actagtgttg aagatactac
     7321 tagtattatt aattggactc ttcatcgtga taatgatgag aagatggatg caacatcgtc
     7381 aataacgatg ttgtgaatgg cttacttact tatcaaatag gtgactaatg atgattgtga
     7441 ataatggtta tctagaaggg agaaaaatga aaaagagaca aaaaatatgg agagggttat
     7501 cagttacttt actaatcctg tcccaaattc catttggtat attggtacaa ggtgaaaccc
     7561 aagataccaa tcaagcactt ggaaaagtaa ttgttaaaaa aacgggagac aatgctacac
     7621 cattaggcaa agcgactttt gtgttaaaaa atgacaatga taagtcagaa acaagtcacg
     7681 aaacggtaga gggttctgga gaagcaacct ttgaaaacat aaaacctgga gactacacat
     7741 taagagaaga aacagcacca attggttata aaaaaactga taaaacctgg aaagttaaag
     7801 ttgcagataa cggagcaaca ataatcgagg gtatggatgc agataaagca gagaaacgaa
     7861 aagaagtttt gaatgcccaa tatccaaaat cagctattta tgaggataca aaagaaaatt
     7921 acccattagt taatgtagag ggttccaaag ttggtgaaca atacaaagca ttgaatccaa
     7981 taaatggaaa agatggtcga agagagattg ctgaaggttg gttatcaaaa aaaattacag
     8041 gggtcaatga tctcgataag aataaatata aaattgaatt aactgttgag ggtaaaacca
     8101 ctgttgaaac gaaagaactt aatcaaccac tagatgtcgt tgtgctatta gataattcaa
     8161 atagtatgaa taatgaaaga gccaataatt ctcaaagagc attaaaagct ggggaagcag
     8221 ttgaaaagct gattgataaa attacatcaa ataaagacaa tagagtagct cttgtgacat
     8281 atgcctcaac catttttgat ggtactgaag cgaccgtatc aaagggagtt gccgatcaaa
     8341 atggtaaagc gctgaatgat agtgtatcat gggattatca taaaactact tttacagcaa
     8401 ctacacataa ttacagttat ttaaatttaa caaatgatgc taacgaagtt aatattctaa
     8461 agtcaagaat tccaaaggaa gcggagcata taaatgggga tcgcacgctc tatcaatttg
     8521 gtgcgacatt tactcaaaaa gctctaatga aagcaaatga aattttagag acacaaagtt
     8581 ctaatgctag aaaaaaactt atttttcacg taactgatgg tgtccctacg atgtcttatg
     8641 ccataaattt taatccttat atatcaacat cttaccaaaa ccagtttaat tcttttttaa
     8701 ataaaatacc agatagaagt ggtattctcc aagaggattt tataatcaat ggtgatgatt
     8761 atcaaatagt aaaaggagat ggagagagtt ttaaactgtt ttcggataga aaagttcctg
     8821 ttactggagg aacgacacaa gcagcttatc gagtaccgca aaatcaactc tctgtaatga
     8881 gtaatgaggg atatgcaatt aatagtggat atatttatct ctattggaga gattacaact
     8941 gggtctatcc atttgatcct aagacaaaga aagtttctgc aacgaaacaa atcaaaactc
     9001 atggtgagcc aacaacatta tactttaatg gaaatataag acctaaaggt tatgacattt
     9061 ttactgttgg gattggtgta aacggagatc ctggtgcaac tcctcttgaa gctgagaaat
     9121 ttatgcaatc aatatcaagt aaaacagaaa attatactaa tgttgatgat acaaataaaa
     9181 tttatgatga gctaaataaa tactttaaaa caattgttga ggaaaaacat tctattgttg
     9241 atggaaatgt gactgatcct atgggagaga tgattgaatt ccaattaaaa aatggtcaaa
     9301 gttttacaca tgatgattac gttttggttg gaaatgatgg cagtcaatta aaaaatggtg
     9361 tggctcttgg tggaccaaac agtgatgggg gaattttaaa agatgttaca gtgacttatg
     9421 ataagacatc tcaaaccatc aaaatcaatc atttgaactt aggaagtgga caaaaagtag
     9481 ttcttaccta tgatgtacgt ttaaaagata actatataag taacaaattt tacaatacaa
     9541 ataatcgtac aacgctaagt ccgaagagtg aaaaagaacc aaatactatt cgtgatttcc
     9601 caattcccaa aattcgtgat gttcgtgagt ttccggtact aaccatcagt aatcagaaga
     9661 aaatgggtga ggttgaattt attaaagtta ataaagacaa acattcagaa tcgcttttgg
     9721 gagctaagtt tcaacttcag atagaaaaag atttttctgg gtataagcaa tttgttccag
     9781 agggaagtga tgttacaaca aagaatgatg gtaaaattta ttttaaagca cttcaagatg
     9841 gtaactataa attatatgaa atttcaagtc cagatggcta tatagaggtt aaaacgaaac
     9901 ctgttgtgac atttacaatt caaaatggag aagttacgaa cctgaaagca gatccaaatg
     9961 ctaataaaaa tcaaatcggg tatcttgaag gaaatggtaa acatcttatt accaacactc
    10021 ccaaacgccc accaggtgtt tttcctaaaa cagggggaat tggtacaatt gtctatatat
    10081 tagttggttc tacttttatg atacttacca tttgttcttt ccgtcgtaaa caattgtaag
    10141 gtgtcgttga aattattaaa taatagaaaa tgactagttt tggcctttcc ctattgtcag
    10201 tcagattagt tattatcaag cttctcatgc caatattaat gcctttaaaa gaacagttac
    10261 aaccattgac cgtacggaga ttcaacgacg gttaggtctg gctaaagcct acaatgctag
    10321 tatttctgga acaagtagtc agtcgactca atctgtgctg agagattctt attctgagga
    10381 gcaaaaaagg cagggtggac tgaatacgct aggatgttag aagtcagaga gcaggttgac
    10441 catgtgatga ttccaaaaat caatcaggat ttaccaatct acgctggttc agaagaggac
    10501 aatctgcaac ggggagttgg tcatctagaa gggataagtt tgccgattgg aggggcttct
    10561 acacatgcgg tcttgagcgg tcaaagaggt atgccagctg ctcggttgtt tgcggatttg
    10621 gataagatga aaaaaggtga ttatttttat gttaccaatc tgaaagaaac cttggcttat
    10681 caagtggatc gtatcatggt gattgaacct agccaattgg atgccgtgag cattgaagag
    10741 gataaagatt atgttaccct tctgacctgt acaccttata tgggctcttt gtcaactgta
    10801 atgggtgact tatcattaac aacgagagag aatcagcttg gttctctctc tttttggatg
    10861 ttcaaagcga tgagaatttt gcttttaaaa tttttaaagt tgcgaaagcc aaaggcttgt
    10921 cgcttgatgt ctttgataag cttgttggtc gcttctaatt tggcattgga aagggatagc
    10981 tgagggcgtt tgtgatgtaa gttttgtact tcatgaaggt cctaaagacc attttaaaag
    11041 cagaattgcc agttgttacg ttatcatcta tgaagctaaa gaattcagtt gttcgctttt
    11101 cttagaaatg aaagagtatg agctgataaa ggtcgttata atagcggagc tcatctgaga
    11161 aaacttttat acctcaaagt cagtctagct ttgatatcat gaagccatta ggagttattc
    11221 cttatctttt agtggcgcgc gatccatata gtgatagatc gagatattta gatccaaaag
    11281 ttctatcatc ctcttttggc gccttttttc cagcagataa tattaaggta gcttggtcta
    11341 acaactccag cactttattt acaccaccta ttaatgcaaa ctacaccact cagattcaag
    11401 ctattgggac aacgattaag tcacaaattc cggaatcgat tttgacggtt acggataaaa
    11461 agagcaggaa gttcagcatt aacaagattg acgaagctaa agaaggctta gtaggtgcga
    11521 ccttcacctt gtctaaacgc acaacagtag cggcagatca tcaagtacaa ggagatttca
    11581 ttcctgtcag caaagagacg acagtcggtc ggacaaccct tacctttgac aaccttaaac
    11641 ctggatttta tgaccttaaa gaaacgaaag cgccgaatgc ttacgtactt gatcctaaga
    11701 cttatgttgt ggtcgttcaa aattcaggaa aaacgacaat tgtggatgaa gctaacttca
    11761 aagaggctga ttacccaatg gctgataata ccagccatgt ggagtgcgta gcgttgctac
    11821 aacgaagcaa agggtaaaaa tcctttattt taagcacttt ttcaagcatt ttgtctttat
    11881 tgaaaagagt gattttaaca taaaaaaggt attaaaaaac atattgacgt gaccgtttgt
    11941 tttgaagtgg cttgcgtaga caaaaaaata gatacgtcag ataaatttct ggcattacga
    12001 gaacattttt agagtgttct ctttttttag tttacggagg aaaaatatat atggaaaaac
    12061 aggattcacg agttctcatc cattgggagg ggaattctgg ggacaagctc attgaacacc
    12121 aaaccagcgc aacggggtgg tactaccaag tcgatcgtag ctttagtcaa ccaaaagggg
    12181 aaccgcccag aatgatccaa aggcactaga aagtgtccgt aatgattcga tttcgggcgg
    12241 tgatgatgtc atgggttatg cttatagcaa atgtacttgg ggagttgcgg cacgaattaa
    12301 tcagtgggac tgaaactcaa aggttgaaat ggtgagaaga ttaccattac cagttcaatg
    12361 ggaaatggtc aggattgggt tggaacagcc gaaagactag atggtgaaac tgatacagtt
    12421 ccaaaagaag gtactattct ctctttttag gaaagtagtt atggttcgta tataggctac
    12481 ggaactatat ctttcgtcac attacatcta cagatagtac catgaatttt gcttatatga
    12541 ccaagtaaag tgaggatata ctaacaaatg aaatatttat tatcgtattt gtccatttta
    12601 tcgaaaagtt tgcatattat cattatgttt gataagatgc aaatataatg atagtaggag
    12661 ctaaatatgg atatttaaaa aatcaagagt atcctaagtg ctttccattt tgaaattcaa
    12721 atatagctaa tagttctaga acttctaatt gtttttcgtc gacgatatga attttcaatc
    12781 ttaactgtta ggattccacc tccctttggt taaagaaaaa aggtcaggtc gtttagataa
    12841 ctttgtcaaa caagctcaag ctatctaaaa atagtttgaa atgggcatta ctctagtttt
    12901 taataagcta tctgatgagc agaaggagaa gttaatgcat gttgggaagt cttattttga
    12961 ctatcaagaa aatgctctta tcccacaatt aggttttcta tattctaaat taactaaaaa
    13021 aattgaactt gataatcggt tgtctccgac tgaacaaaag ttattgatta ccttattatt
    13081 acatactaaa ggtttaatca ttgatatgta agaagtaagt cagctaaccg atctttctat
    13141 tctaaaactt atattgttgc tttagaaatt ttaaagagcg tggatggctt cataataaac
    13201 agaaatctta ccaatttgcg aagccaaaaa atatgatatt tgaagagtcc aaagatctaa
    13261 tagatagtcc agttagagaa gcgttgatta taagtgataa ggattttcaa aaattaaaac
    13321 aagagctatt attttaaccg acttatttta aagacttatc atatctaggc ttgcttgatg
    13381 attcggaaaa atacggagac tatactattt caaggaaaag atacaaaagt ttcgaatcaa
    13441 gtcttcaact atacatcctt caaagtcatc ggctagagat ttggaattat gaaccaatcc
    13501 ctttgattac tagaaaaata aatagcttgg agaggctaac gtgacactgg ttgatccaat
    13561 ctcgctttat ttaacactaa gaatgatgaa gaccctcgta ttgaagaaga agttgagcag
    13621 ctagaagata agattttgag aatataatcg ttttttcgtg ctacactaac ttgtcagggt
    13681 tgcaacagac aaaaaaataa tttaggaggg attcgttatg cgtacaaagt atataggata
    13741 tgaaattgtt ttaaggggtg gttattaata tggataagaa aaaggttgtt tcaactatcg
    13801 tatctacagg gtttcttata gtttcaatat caaatttttt tagagagcta tcacctatac
    13861 tttttctctt gaatattatt ggatttttct gcttcctatt attattttct gccaacataa
    13921 ataaattgtt cttaaaacga tagccgttat ataggatatg gtatgtatgc atttggtgaa
    13981 attataggta aaacgaatgg aatagtactt actcctcaaa gctatcgtga gagatataaa
    14041 aggatatcga ggagtcacta gatagttgga tctcattgaa aaaatttgtt taaagtatct
    14101 catataagca taaaacgagg aatcaatcaa aatttcttga ttgattcctt gttttttggc
    14161 tctttgtcaa ctgtagtggg ttgatgaaaa attatattct ggagaaaacc aaattggttt
    14221 tctccttttt gatattcaaa gtgatatgaa ttctcttttt aaaatattca aagtttctaa
    14281 agctaaatgt ttgtgcttga tgattttgat gagattattg gttgctcaag tttggtgttt
    14341 gaataaggcg attcaaggac attgataacc ttatctctgt ctttaaaaaa tgtcctaaaa
    14401 acagtttaaa agattggagt gacactagag aaagtgtttt aaacagggta aagaaatggt
    14461 gagcttgctt ctcttgaaag taaagcagga ggagttgata gagtttcata atgtttttta
    14521 tagagaagcg atatcttctt atcttgaaac agaaaatttt gagaaggcta aagtgactga
    14581 agagaattac caagtttttt taggaatccg tacttatcag aagagttata actatcaaga
    14641 tcatagtgag aatctaaaag gaaatattga ggtagcaatg ataggagttg aaaataatat
    14701 tagcgacgaa catatttttt gctagaatat ataaaatgtc tttgaaataa tttagccctg
    14761 tgttatgata ttaaggaggt tatttatggt acgtaaattt ttaaaatcgc tcctatctct
    14821 ctttcttata gcagtaatcg ccaccggtat ttcagtggca tgtttctttt ttattccaga
    14881 aaataaaggg aatattacac cgattttatt gcatcgcttt atgcgaaaaa ataatgttaa
    14941 tggaatgatg attgttagcg ataatactgg taaaccgatt accatctcgc atgggattaa
    15001 ccgtggggaa gttgaaacgg atattgaaaa taataaatta tttccaatgg cttctctcca
    15061 aaaactcatg acaggaatta ttatccaaag attaattgat caagatgtct tgagtgaaga
    15121 cgaccgattg tctcagttct ttccccaagt aaaaggtagt aatagtatta ccatccacca
    15181 attgttgacc catacgagtg ggttaagaga aaaaggtgtt aaggtgtctc cttatctaaa
    15241 aaatgagaga gaacaattac aattttgctt aaaacactat aactttgtta ataagaaatc
    15301 gtggtattat tctaatatca actttagttt tctaacaggt atagccactc aagttacggg
    15361 aaggacctac gctgaacttg ttgatgatgt gataaaaaat cctttgcgat tagatgatac
    15421 ccaaagttac caatcagttg ttaatcatga cttagttagc ccaatgcgaa agaacggcaa
    15481 gttaaataaa attaatatct ttaatcaagt atccacagca tatggagctg gagatttttt
    15541 cacaactccg ctgaactttt gggtgttgat gcggtctttt tctaaagggt actttttccc
    15601 aacggatgaa tataccaaac atcaaaatga tgcgatatca cattattatg gtgggcttta
    15661 catgcatggt cgtatagtta attctaatgg ggcctttttc cagtattcaa gttttggtta
    15721 tgcagatcta aagacgcatg atacaatggt gctttttatg aataacaaag tctatagtga
    15781 tgctagtcac gtggcaccaa aagcatttga atattatcaa aaatttatgt tccttaataa
    15841 gattttccac ttagtctttt actttgtatt ttctttcttt ttaatgcttt taatacgtca
    15901 ttcgtttagg aaaagaaggt ataagaaacg tctctaaatc acatgaataa aaaagaaggc
    15961 taagttggcc ttctttttta ttcaatatta agcagtaaca ccatctaaga aaacgtttgc
    16021 attgaatttt taattgtgct ataataaata gctgttaatc attattatag tttaataatc
    16081 taactgtcga gctgatttaa gctataaaaa tactgttgag taaaagaggt attaatatgt
    16141 caatgattga agtttctcac cttcagaaaa attttataaa aaccgttaaa gcaccagggt
    16201 taaaaggagc atttcaatca tttcttcgtc ctgaaaaaca cacctttgaa gctgttaaag
    16261 atttaacatt tgatgtgcct aaaggacaaa ttttgggatt tattggagcg aacggtgcag
    16321 gaaaatctac aacaattaaa atgttaactg ggattttaaa accaacttcg ggcttttgcc
    16381 gtattgatgg caaattgcca caagaaaacc gacaaaatta tgttaaggat attggagtcg
    16441 tttttgggca acgtacgcag ttgtggtggg atttagctct acaagaaacc tatacagtgt
    16501 taaaagaaat ctatgacgtt cctgataaag aatttcgcaa acgcatggct tttttaaatg
    16561 aggttttgga actaaatgac tttataaagg atcctgttag aactttgtct ctcggacagc
    16621 gtatgagagc ggatatcgca gcttctttat tacataaccc aaaggttctt ttcttagatg
    16681 aacccaccat tggcttggac gtttcagtga aagataatat ccgacgtgca atcacacaaa
    16741 ttaatcaaga agaagagaca acgattttac taacgacaca tgacctcagt gatattgaac
    16801 aactttgtca tcgtattttt atgattgata gaggacaaga aatatttgat ggcacagttt
    16861 ctcagctaaa agagacattt ggaaagatga aaactctttc ttttgatttg cgcccaggac
    16921 aagagcatat cagctctagt ttaattggaa agtcggagat taatattaaa cgtaatgact
    16981 tagttttaga tattcagtat gatagttctc gctaccaaac tgctgacata attcagcaga
    17041 cattagcaga tttttctgta tgtgacctta aaatgacaga tgcggatatt gaagatatca
    17101 ttcgtcgctt ctatagaaat gagctataag gatgaacaga atttggagac gttataaacc
    17161 ttttattagt acaggaatcc aaggattaat cacatatcgt gttgatttca tcctttatag
    17221 gataggtgat gtgataggag catttgttgc attttatcta tggaaagcag ttttcgattc
    17281 ctcaagtcaa tccttaatcc aaggttttca gctgtcagat atgattctct atattattat
    17341 gagttttgtg actaatttat taacaagaac tgatagttca tttatgatag gtgacgaagt
    17401 aaaagacggt tcaattatta tgcgactctt acgtccagtt cactttgctg cttcctacct
    17461 tttcatggag attggttcta gatggctcat ctttttaagt ataggggtac cgtttttgct
    17521 tgttattacg ggggtacgcc tcttcttggg gactgatttg attcaagcta tagtgttagt
    17581 ggtattttat attattagca ttattttagc ttttttgatt aacttctttt ttaatatttg
    17641 ttttggcttc tcggcatttg tttttaaaaa tttatggggg tcaaacctgt taaaaaattc
    17701 gttagtcgca tttatgtcgg ggagcttgat tcctcttact ttttttccga aaatcgttgc
    17761 tgatatttta ggatttttac cattttcatc tttaatttac acaccagtaa tgattattat
    17821 tggtaaatat gatggcagtc aaattgtgca agccttactt ttacaaatat tttggctaat
    17881 agtaatggta gctttatctc aattaatatg gaaaaaagta cagtcgcata tcactatcca
    17941 aggaggttga gatgacaaaa tatcagcgaa tgcactttat ttttattaaa caatacatga
    18001 agcaaatcat ggagtataaa atagactttt ttgtaggcgt tttaggagta tttttaacac
    18061 aagggctaaa tcttttattt ctgaatgttc tttttcaacg tatcccttct ttagaaggat
    18121 ggacttttca gcaaatagct tttatttatg gtttttctct cctcccaaaa ggaattgatc
    18181 accttttctt tgataacttg tgggctcttg gtcaaaggct tattcgaaaa ggagaatttg
    18241 ataagtattt aaccaggccg atcagtcctt tatttcatgt cctagttgaa acctttcaag
    18301 tagatgcatt gggggagtta ttggttggtt ttattctgtt atctaccaca gtatctagta
    18361 taagctggat cgtgccaaaa gtattattat ttatatttat tatccccttt gcaactttga
    18421 tttatacctc cttgaagatt gctacgtcaa gtatcgcttt ttggaccaaa caatctggtg
    18481 ctgttatcta tattttttat atgtttaatg actttgctaa gtatcctgtc gcaatatata
    18541 ataatctatt gcgttggatc attagttttg tcattccatt tgcttttaca gcttattatc
    18601 ccgctgctta cttcttacaa gatagaaatg tctattttaa tattggtggg gttatcttga
    18661 tttccttgat ttcttttatg gtatcactaa tactttggca taaaggagta gaagtatatg
    18721 agagtgcggg ttcttgatta gtaaggcaag ccattaattt ttttgagtgt ttagaagttt
    18781 taggcttact aacttagctt tgttagtgat gatatactcc ccttatagtg tctagtgaat
    18841 ttttgttttt ttactgtcca ctataagggg agtatatcag gtgaagaggg actattagtg
    18901 ttgagaataa tgtccctcat agaacaataa ttaccatatc tttaccaaaa taaagttaaa
    18961 aacgctctta gatgtgcttt ctaagagcgt tttttggtct ataaaaggta agcaaaaagc
    19021 agatgtcatt tatgaaagtg ttgacaaaga tgaaatactt tgttactatt ttaacataat
    19081 aatgatattt taattagagt gtgaatttat aattaatttt tacaatgttt attttaaatt
    19141 taaactaatg tttatagcag tgttttactc atgattttgt gaattcaaca cttaagtcat
    19201 gacctcattt taaagagttt cagacgacat agaaaggaag aaaacataag aaaggcaatg
    19261 cacggcttgt ccatagtagt gtttgaagta gatgtttaga aatattttat tttccaacag
    19321 tactgatatc agagcgaaga tgattagttg aaggtgataa tatgggacgt tctaatattt
    19381 tgagtaggtg tgaacaatgt tctgataatc aatgtggctt gtataaaacg cggcttaaag
    19441 acaagttact attagtagtc caactggttg gaaatattag gtattctgag tttcttacgg
    19501 aaggtggtgt atatcataag ggagaaacct tgttggaagt tataacctta ggaatttctt
    19561 atgaatataa aagttatggg gatatcaaag taatcaagaa gtactgtgct gatggcagtg
    19621 tcgttgatta taacatgcca atactattaa tggaaaggat tgaataatga aaacagtaat
    19681 aatatatgga ggacagggta atgttcagtc aaaacacctt tgtcaactag ttaaaactcc
    19741 aaaaggtgaa attctttttt caaagttaca agcaactgat ttagaatcat atcagttaat
    19801 gatggatgtc attaaccaca aggtaagttt agacaatgtt catgcgtcta tgttagcaaa
    19861 ttttttatat aatgattggc taacaaacga tgaaaaaata gtatctggga taggattttc
    19921 agctcatagc gccggtattt tcaatgtgtt atatgctagt ggtagtacta gttttgaaaa
    19981 cattattcac tttattaaac gtcgtgccca acttatagaa aattgtcatg tgtctgaaga
    20041 gctttggctt ttgatggttg aaaacattga tatgtttagt aaggatgttt taaaagaaca
    20101 gtctgagaaa gtaaaattag ctattattac tgatgataga gctggtgtaa ttgctatgga
    20161 tcaagtcaac cgttctttct ttgatgacgt gatgactaac aaaggttatt tttataaatt
    20221 aaagcagtta ggcgtaaaag caccatatca tactgatttt ttaaatgaat ctaaagagca
    20281 gtataaacaa ttgattgaaa gcctagatat ttcgcaaaat gatgactatg actatatttt
    20341 tcattgtgat caattggtag atgaaatttt atatcagtgg accaatctct ttaactggcg
    20401 tgcgattaag gaaactatca tagaaaagga tgcggagatt attgatattt ctcctaatag
    20461 gtttatttct agtcagttgc aaaaaatgaa aaagaggggg aggaaacagt tatgaaagta
    20521 gcaatagtta ctggtggtac cagaggaatt ggaagggcta ttacaaaaga gttatacaaa
    20581 gaaggttaca aagtcattgc tatttataat agcaatgatg ctaaggcaag agctttacaa
    20641 gaggagttac caaaattaga cgtttataaa tgcaatattt cagatgcaaa agcagtacaa
    20701 aaattggtga ctaaaatttt tagagaatat ggcggtattg attgtttagt taataatgct
    20761 ggtattgtta gagatggctt tttcttgatg atgtctaagg aaaaatggat ggatgtcatt
    20821 aacataaata ttatggggct agttaacatg agtaaagccg tgttaaaaat catgaaggct
    20881 aagcgtatac agggaaaggt tatcaatatt tcttcaacta gtggtatagc agggcagata
    20941 gggcaagcga actattcagc aacgaaaggc gctatcattt ccattactaa aactctagcg
    21001 aaagaatttg catcagatgg tattacgatt aattgtgtaa gtccaggatt cattgaaaca
    21061 gatatgacaa atgaattgca aaataaagaa gagctaaaag agcatcttat tcctctaaaa
    21121 aggtttggac aacctgaaga ggtggcttgg ctcgtaagct ttttagcaag tgagaaggca
    21181 aactatatta caggaaaaaa tattgtgatt gatggaggaa tgattaatga ttaactgggc
    21241 tgaagaacgt aaaataataa aaacacgtca agaagtagtt gatgaaatta aaggtatttt
    21301 gattgactct ttaatgttgg atattgataa agatctgatt atgaatgatc aaccattatt
    21361 tggtcgtgga ttagagctag actctattga tgccctagaa ctgtcaatag ggatttcaac
    21421 aacgtttggt gttgaactaa atgatgatga tatttcaata ttatcatcag taaatagact
    21481 agcagatttt gtgattgaaa atagtgagga tatagatgac caagctaagt agtgaagaaa
    21541 ttataaaaat gttacctcat cgctatccgt ttctaatggt ggatagagtg cagagttata
    21601 aaaaaggtat cattgaatgt cgtaagaata ttactatcaa cgaaccttac tttcaaggac
    21661 attttccaca acgtcctata gttccgggtg ttttgatgat tgagatggca gctcaatctg
    21721 cagctttact ttatatttta gatgcacttg atggtagcct cccctctctt gatgaattaa
    21781 cagcagagaa aatagcagaa aaagtaggtt atttagccag tgtgaagaat ttcaaattca
    21841 agaaaattgt tactccagga gaccagctaa acattacatg taaatctcaa tcaaaattgg
    21901 ggcatctttt agaaattcaa gtcgttatta gagatgagaa taaaaaagag gttgcctcag
    21961 gaaggatgtt ggtatcagaa aatggaaatt aaactcaaaa atattggaaa aaaatatggt
    22021 tcttttgaag cattgagaga cattaatctc atctttgaag agggaaaatt ttatggcctt
    22081 cttggaccta atggagctgg taaaacaact ttatttaacc ttttaattca aaattttaag
    22141 caaacgtcag gcgatattaa gtgggaggtg gatggaaaac ctctatctat taaagatttt
    22201 tatcgtcaca ttggtattgt cttccaaagt aatcgcttag atgataattt aacggttgag
    22261 gaaaatctaa tttctagagg tgctttgtac ggtttatcta aatcacaagt acgtaatcgc
    22321 cttaaagatc ttcaaacgta tctagatatc acagcaataa aaaaacaaaa atatggcagt
    22381 ttatcaggag gtcaaaaacg aaaagttgat attgcacgcg ccttgctgcc acaaccttct
    22441 ctattattat tagatgaacc aacgactggt ttagaccctc aatctcgtcg cgatttatgg
    22501 gatgctatcg cacaactaaa tcaacaatcg cagatgacag ttgtactaat tactcattat
    22561 cttgaagaaa tgtctgcttg tgatgtttta aatgttctta ttgaaggaaa catttattat
    22621 tcgggagata ttaaaagctt tattgagcaa cattccacaa caaacttgaa tgtggtattg
    22681 aaaccagaaa agtcattaga ccaactttcg atagctgatt ttgttaataa atgtcaagtc
    22741 ctttcagaaa gggagatagt atttaaggat atttctgttg aggaaatgat gcaggttatc
    22801 tcagacaatc aagataaatc tattattgag acatttaatg ttgaatattc gaacttagag
    22861 gcggcgtatc tcaacttgct tagagaaaaa gaaaggaaaa acaatgtttg aactaatttc
    22921 tagaaatcgt aaagtttaca cgcgagatcg gttagcgttt ttcatgtctt ttttatcagt
    22981 tattatctta attttggtct atcagatttt cttgggaaaa attcaattag atgctataaa
    23041 agcagcattg aggagtaata cagtatccag tgatactatt cacatggtta attattggct
    23101 cattgcaggt ttgacgacta ttatttcaat gactagtaca ttaggtgcct ttggagttat
    23161 ggtgtccgac aaagaaaaaa aattaaatga agattttaag gtcagtccta tatcaaatat
    23221 caaaattgaa ttgtcctatg ctatatttgc cgttttattt ggtattgtca tgacgatgtt
    23281 ctcttgcatc tttgctatcg gtatttttaa cggctttaat tccttgctag agtattcagc
    23341 tatggattat ttaactattt tagggatcgt ttccttaggg actgttttat ctgcggcgat
    23401 gattttacct attttagcct ttattcatac tagttcagct tttacgactc ttagcacaat
    23461 tgtagggact tttattggtt ttatttcagg agtatatctc tcaattggct cggtaggaaa
    23521 agcattacaa caggttatga catggtttcc attgactcaa atcaattctc ttttgaaaca
    23581 ggtcttaatg aagggttcta ttgcgaaggt atttgacaaa gccaacgaag ccactgtctc
    23641 taactataaa gaatcatatg gtgttgtttt gcgtaatgct gatggagaaa ggctgagtaa
    23701 tcactttatg ttgatttata tcattgccct cattcttatt ttattggcaa ttcattttat
    23761 tattaaaaag gttaaaaaat gaaagatgat aataaattaa agatttctga agcttcctta
    23821 gaagattatt ctgaagtggt tcatttattt aataggaatc atgtttatca atttccagac
    23881 ggtaggcctt taactgttga tgaccttgat ttaacgttaa aagttaaaga agtgacacat
    23941 ttatttttac tgaaaaatca tggtgttttg attggaacat cagctttctt taaatttatt
    24001 acctatggtt gtttagattg gaatagtagc tttagtggtt ttcttttaat tgattcgaaa
    24061 agtcgtagtg gacaggcaat cacttatcta tataaaacta tcttaaagaa gataacaaaa
    24121 ctgaagtttt ctaatatcta taccgaaatc agtaactaca ataaaccctc cttagcttta
    24181 tcaaaattga atggcttcaa agagtatgat aagacatatg aagacatact gcattgtcga
    24241 tcattgcgta gtcacctccc taagatattg aatacgtttc gtatttcgaa ttattatggt
    24301 aaaacatacg acatatcaac ttttcaaatt atggaggaaa ttgaaaatcc cttggaagag
    24361 gagacagaaa ttaggaccaa agtctctgat gaagaaatat tatttaaagc agaagatagt
    24421 gcttcacttc cttattattt aaaaatgagc ttgtttcaaa tggaaattgc taagttagat
    24481 aaccgttatg ttttacaagt tgacttttta tcggaacaag taaagagggt tcgggttaaa
    24541 actggaaaat atcacttggc aaacttaacg agggctcacc ctagcctaac tttaagtcgt
    24601 tttgccaatt attattacat tcaagcaact gtggaaaccc tttatggtaa tatcgatgtt
    24661 caactggaac gtcgaaaaaa gcattataga gatgcaacta tttgtcttaa gagaactttt
    24721 caaggttatg atttacttat ctctccaaat ggcagcctta tttttgaaaa gcagaaaaga
    24781 aagatacttg aagatagttt tttgattttt agccaacctt tggacaagaa attagttgta
    24841 aaagaagaag aaaatcacat tacaatcaaa tgtttctatc aaggtgcttt aattgagaaa
    24901 atcatgacat ttacaagtga cgaagaaatc acttgtgttt ataaatgtaa tcaaaaggct
    24961 aaagagatgt tcccaaaatt attgaaacag actttcaagt tacactgcca agagcagttg
    25021 attagagata gtgaaggtta tcttgtgaat gttccaggaa gttacccgat tgagcatgat
    25081 gattttcttc gagcagataa gtttgaagat agacaattcc attattacct cccgaatgaa
    25141 gataaaatga tttcctattc tcctcctggc aaagccagca atcaaatgca atttagaccc
    25201 ttatgtctta ttgatacaga tagcctttct tttccactga cttatcattt taggatttca
    25261 caagcatcat cggaggaggc gttaatcaat ctaaaaaaac agccaatatg ggacagcaac
    25321 taccaagcgt cagcaactga ccttttaaaa catatctcta acctaacatt agaagaagag
    25381 aaggattatg gcatcaagag aatgatagct aacagaaagc attatcctag tcacaaactg
    25441 gttttagcct ataatcagat tgttttgcct aagaatgaaa ttccaagaga cagtgaatta
    25501 tattctattt cttttgatta tcgaattaga ggaaagtttg tccaaatccg ccaaggggaa
    25561 catgtcaaat atgataataa atcctatgtt ttggaaaata gtcaacaatt agtattgtat
    25621 gtagctagcg atgataaata tatactaatt tctgctaaaa atggaatatt ttattcttac
//
LOCUS       DATROQ010000019        25615 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.019, whole genome shotgun sequence.
ACCESSION   DATROQ010000019 DATROQ010000000
VERSION     DATROQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25615
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.019"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..953
                     /gene="fbsB"
                     /locus_tag="VBC94_000730"
     CDS             <1..953
                     /gene="fbsB"
                     /locus_tag="VBC94_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibrinogen-binding surface protein FbsB"
                     /protein_id="HEO6979016.1"
                     /translation="YLENKEKFLVPNIPYKNKLILREEDKYSFEDDEEEFGNELLSYN
                     KLKNEVLPVNITTSTILKPFEQKKIVEDFNPYSNLDNLEIKKIRLNGSQKQKVEQEKT
                     KSPTPQKEAVKEQTEQKISGKTQNVEKKSETVATPQQSSTAQTSVQQPAPVQPVVQES
                     KASQEEINAAHDAISAYKSTVNIANTAGVTTAEMTSLINTQTSNLSDVEKALGNNKVN
                     NGAVNVLREDTSRLENMIWNRAYQAIEEFNVARNTYNNQMKSETVPVDNDIEAILAGS
                     QAKISHLDNRIGARHMDQAFVASLLEVTEMSKSISSRIKE"
     gene            1020..1565
                     /locus_tag="VBC94_000731"
     CDS             1020..1565
                     /locus_tag="VBC94_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979017.1"
                     /translation="MKKITTLILASSLLLVATTSVKADDNFEVPTRYVKMSEKSKAFY
                     QRLQEKQRKTHTTVKTFNNSEIRHQLPLKQEKARNDIYNLGILISQESKGFIQRIDNA
                     YSLENVSDIVNEAQALYKRNYDLFEKIKSTRDKVQVLLASHQGNTDLKNFYAELDDMY
                     EHVYLNESRVEAINRNIQKYN"
     regulatory      1716..1805
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1961..2527
                     /gene="thiW"
                     /locus_tag="VBC94_000732"
     CDS             1961..2527
                     /gene="thiW"
                     /locus_tag="VBC94_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEO6979018.1"
                     /translation="MTNHKTKKLTVLAIMIALDVFLTPIFRIEGMAFMSSVVNIIAGV
                     IMGPIYALVMALVTAFIRILTQAGVASVAPLAILGAGPGAFLAGWFYRFYAKDWMSWV
                     GEFIGTGIIGALLSAPIMVWFWTWTADGNHEVLAKASAAQSIFLFMPRFIGATIIGGV
                     IALLLLQGLKRVPVFMTIQALFKGEKVK"
     gene            2524..3078
                     /locus_tag="VBC94_000733"
     CDS             2524..3078
                     /locus_tag="VBC94_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6979019.1"
                     /translation="MKLGLKDVLLIALLAVVLGVVYFGAGYISNAFVPFVGPIAHEVI
                     YGIWFVAGPMALYILRKPGTAIVAELLAALIEVLIGSIYGPSVLVIGTLQGLGSELGF
                     TLFRYHNYKLPAFILSAILTSIFSFAWSFYANGLSAFSFSYNILMLIVRTVSSIIFFL
                     LTKNICDQLHRSGVLNAYGIHEGK"
     gene            3082..4368
                     /locus_tag="VBC94_000734"
     CDS             3082..4368
                     /locus_tag="VBC94_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6979020.1"
                     /translation="MLSVEKLAYTHGDSHYLFDEVTFSLNPGERILISGYSGCGKSTL
                     ALLLSGLKESGKGQVLLNGSLIEPSDVGFLFQNPDLQFCMDTVAHELYFILENLQIEP
                     EQMQDRSEFVLAQVGLKGFQNRLIYTLSQGEKQRLALATIFLKSPKLIILDEAFANLD
                     QESASQLLQLVLNYQANNQSMLIVIDHLITYYQDIMDHYFWLEKWLIRVDFDYMLNRL
                     NVFELEKKSHNTGDKLLSIKDFQVKLSKNKFISYLDFDLASGERLCLDGPSGVGKSSF
                     FMGLLGLYRTKGKKQFTHRKQIPISFLFQNPLDQFIFSTVYDEIFQVCKDSNKARDIL
                     ETINLWDKKQFSPFQLSQGQQRRLAIGSILASDSKLLLLDEPTYGQDAYHANMITTLL
                     LSYCHKNHCGVIFTSHDPHLKECFATRFLEVSSCSI"
     gene            4356..5024
                     /locus_tag="VBC94_000735"
     CDS             4356..5024
                     /locus_tag="VBC94_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEO6979021.1"
                     /translation="MFNLKNYHPAITFLCLLIPTFSFSFTLRLRTSLLFLIIVVTLQC
                     FVKVSLKTWAKVNLISFVMGLSLFLGTYFWGKLPHQFVLASLVACRPLIFMNVGLLFH
                     ASHSNYDFIESLYQTFKVPSHFAYGIFAVFNLLPLIKLQYQRNRLAFRLKNQVTWALS
                     PRLILSVLLKTIYWVEQLELAMLSKGFEVGKERTHASTYPVRFRDYSLLGMSILLAIG
                     MIFK"
     gene            5108..5788
                     /gene="tenA"
                     /locus_tag="VBC94_000736"
     CDS             5108..5788
                     /gene="tenA"
                     /locus_tag="VBC94_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEO6979022.1"
                     /translation="MLKDEMIAKGTKYIQSIYQDPFIQGIVKGRLDHDVICHYLQADN
                     IYLGKFADIYALCLAKSDNLRDKQFFLEQVDFTLNRELADGEGPHQALAAYTNRSYQD
                     IIEKAVWYPSADHYIKHMYFHFYENGIAGALAAMSPCPWIYHQLAKKIIEENQFLNGN
                     PFNNWITFYANDTVEELMENYFRMMDYYAQNLSKEKQADLVDAFVKSCQHERRFFQMA
                     INQEKWED"
     gene            5790..6587
                     /gene="thiD"
                     /locus_tag="VBC94_000737"
     CDS             5790..6587
                     /gene="thiD"
                     /locus_tag="VBC94_000737"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO6979023.1"
                     /translation="MTYLAPVLTIAGTDPSGGAGIMADLKTFQARRTYGMAVVTSVVA
                     QNTCGVRGVQHIETAIIDQQLACVYDDIKPKAVKTGMLAERETISLVASYLKKYPQPY
                     VLDPVMVATSGHRLIDSDAVEALKEDLLPLATIITPNLPEAEVLVGYDLSDEVSIIKA
                     GYDIQKQYSVRNVLIKGGHLDGLAKDYLFLEKEGLITLSNQRINTIHTHGTGCTFAAV
                     VAAELAKGQSILNAVSTAKSFITSAIETAPELGLGNGPVNHTSYQGD"
     gene            6589..7359
                     /gene="thiM"
                     /locus_tag="VBC94_000738"
     CDS             6589..7359
                     /gene="thiM"
                     /locus_tag="VBC94_000738"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEO6979024.1"
                     /translation="MYLEQLKEVNPLTICITNNVVKNFTANGLLALGASPAMSECIED
                     LEDLLKVADALLINIGTLTKESWQLYQEAIKIANKNQVPVVLDPVAAGASRFRLEVSL
                     DLLKNYPISLLRGNGSEIAALVGEKQASKGADGGKVADLESIAVKANQVFDVPVVVTG
                     ETDAIAVRGEVRLLQNGSPLMPLVTGTGCLLGAVLAAFIGSSDRSDDLACLTEAMTVY
                     NVAGEIAEKVAKGKGVGSFQVAFLDALSQMKSEMIMDK"
     gene            7373..8044
                     /locus_tag="VBC94_000739"
     CDS             7373..8044
                     /locus_tag="VBC94_000739"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001904425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEO6979025.1"
                     /translation="MKDTLKLYFVCGTVDCSRKNILTVVEEALQAGITLFQFREKGFT
                     ALQGKEKIAMAKQLQILCKQYQVPFIIDDDIDLVELIDADGLHIGQNDLPVDEARRRL
                     PDKIIGLSVSTMAEYQKSQLSVVDYIGIGPFNPTQSKADAKPAVGNRTTKAVREINQD
                     IPIVAIGGITSDFVHDIIESGADGIAVISAISKANHIVDATRQLRYEVEKALVNRQKH
                     SDVIK"
     gene            8171..9430
                     /locus_tag="VBC94_000740"
     CDS             8171..9430
                     /locus_tag="VBC94_000740"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO6979026.1"
                     /translation="MRKIIINGGKQLTGEVAVSGAKNSVVALIPATILADDVVVLDGV
                     PAISDVDSLVDIMETMGAKIKRYGETLEIDPRGVKDIPMPYGKINSLRASYYFYGSLL
                     GRYGQATLGLPGGCDLGPRPIDLHLKAFEAMGASVSYEGDSMRLATNGKPLQGANIYM
                     DTVSVGATINTMIAAAKANGRTVIENAAREPEIIDVATLLNNMGAHIRGAGTDVITIE
                     GVKSLHGTRHQVIPDRIEAGTYIAMAAAIGRGIKVTNVLYEHLESFIAKLDEMGVRMT
                     VEEDSIFVEEQEKLKAVSIKTSPYPGFATDLQQPLTPLLLTAEGNGSLLDTIYEKRVN
                     HVPELARMGANISTLGGKIVYSGPNQLSGAPVKATDLRAGAALVIAGLMAEGRTEITN
                     IEFILRGYSNIIEKLTSLGADIQLVEE"
     gene            9532..10086
                     /locus_tag="VBC94_000741"
     CDS             9532..10086
                     /locus_tag="VBC94_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6979027.1"
                     /translation="MDIWTNLGRFAFIETEHVNLRPVAYTDREAFWRIASKRTNLQFI
                     FPVQTSKKESDFLLVHSFMKEPLGVWAIEDKVSHKMFGVIRFENIDLSKKTAEIGYFL
                     KESSWGQGIMTECLKTLSFFAFQEFGMDKLIIVTHKENIASQKVALKAHFKQSRSFKG
                     SDRYTRRIRDYIEFQLTRGDYRYE"
     gene            10079..11362
                     /locus_tag="VBC94_000742"
     CDS             10079..11362
                     /locus_tag="VBC94_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6979028.1"
                     /translation="MSKHQEILEYLENLAVGKRVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETRPRSGTVRVAQKAKVNIEKLTYAEIARISDSQVVAGTEGLSKEFSKFSIGA
                     MTHRNIEKYLVQGGLLIVGDRDEIQHLALQHQNAILVTGGFNVSPSVCRLADKLQIPV
                     MVTHYDTFTVSTMINHTLSNAKIRTDLKTVEQVYQSQMDYGFLAQDDTVKEFNLLVKQ
                     TKNVRFPIVNQANVVVGVVSVQDILGKDKEVKLATVMSKNIIVAKPRMSLANISQKMI
                     FEDLNMMPVVSDDFELLGVITRRQAVENLSMSQGTALYTYSDQILSNLQIEDGHFSFL
                     VEPAMIDHTGSLTQGVLTEFLKEICIRVLTRKHQRSIVVKQMTLYFLQPVQIDEIIMV
                     TPTIISEKRREATLDLELKLENKIIAKAMIAVKIN"
     gene            11385..12245
                     /locus_tag="VBC94_000743"
     CDS             11385..12245
                     /locus_tag="VBC94_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEO6979029.1"
                     /translation="MITLKSAREIEAMDRAGDFLASIHIGLRDIIKPGVDMWEVEEYV
                     RRRCKEENVLPLQIGVDGAVMDYPYATCCGLNDEVAHAFPRHYTLKQGDLLKVDMVLS
                     EPLDKSIVDVSSLNFDNVAQMKKYTETYSGGLADSCWAYAVGEVSQEVKDLMSVTREA
                     MYIGIEKAVIGNRIGDIGAAIQDYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLREGMVLTIEPMINTGTWEIDTDMKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            12247..13167
                     /locus_tag="VBC94_000744"
     CDS             12247..13167
                     /locus_tag="VBC94_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEO6979030.1"
                     /translation="MKLKKFFEDLLAKLEYRPIQVFMRHFQSAEMDLSAIAVAYYLLV
                     TAFPLLVIAANIFPYFHINVSDLLSLMQKNLPKNIYEPASRLAVDAFSKPSTGILGFA
                     SLTAFWTMSKSLTSLQKAINKAYGVDQHRDFVISRLVGVGTGLIILFLLTFVLIFSTF
                     SKPVLQIIVNMYDLGDTLTAWLLNLAQPVTFLTIFLGIGILYFILPNARIRKVRYVIP
                     GTLFSTFVIGFFSNLISQYVLNRVEKMVDIKTFGSVVIFILMLWFIFLAHIMILGAIL
                     NASVQEIATGKIESRRGDIMSLIQKSKEEK"
     gene            complement(13184..13639)
                     /locus_tag="VBC94_000745"
     CDS             complement(13184..13639)
                     /locus_tag="VBC94_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEO6979031.1"
                     /translation="MVKLKSLLKKSIQNEVSLYLLFGLLTSLLYLVIRQGIFNFSQDA
                     PFSAIVANIIAILFAFFTNDRFVFKQTKIGQFQRLQTFVIARLGTLGLDLILAVIFVD
                     QFPSIIGQFVQHNLNKINTIESLVSQILIILLNYILSKFVIFKDKKRQL"
     regulatory      13710..13823
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            13821..14330
                     /locus_tag="VBC94_000746"
     CDS             13821..14330
                     /locus_tag="VBC94_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEO6979032.1"
                     /translation="MNTFTTRDYAHMAIVTAIYIVLTITPPFNAIAYGAYQFRVSEML
                     NFLAFYHRKYLFAVTLGCMISNLYSFGMIDVFVGGGSTLLFVYLGTILFKQYQKDYLF
                     NGLINKAFFFFSFFFAASMITVAVELKIVAGLPLLLTWLTTAVGELASLLVGAVLVDK
                     LSRHVDFTK"
     gene            14470..16428
                     /gene="ligA"
                     /locus_tag="VBC94_000747"
     CDS             14470..16428
                     /gene="ligA"
                     /locus_tag="VBC94_000747"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEO6979033.1"
                     /translation="MENRMNELVSLLNQYAKEYYTQDNPTVSDSQYDQLYRELVELEE
                     QHPENILPNSPTHRVGGLVLEGFEKYQHEYPLYSLQDAFSKEELIAFDKRVKAEFPTA
                     SYMAELKIDGLSVSLTYVNGILQVGATRGDGNIGENITENLKRVHDIPLHLDQSLDIT
                     VRGECYLPKESFEAINIEKRANGEQEFANPRNAAAGTLRQLNTGIVAKRKLATFLYQE
                     ASPTQKETQDDVLKELESYGFSVNHHRLISSSMEKIWDFIQTIEKDRISLPYDIDGIV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVVVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPNQIKERLAHFASRDAMNITGFGPSLVEKLFD
                     AHLIADVADIYRLSIEDLLTLDGIKEKSATKIYHAIQSSKENSAEKLLFGLGIRHVGS
                     KASRLLLEEFGNLRQLSQASQESIASIDGLGGVIAKSLHTFFEKEEVDKLLEELTSYN
                     VNFNYLGKRVSTDAQLSGLTVVLTGKLEKMTRNEAKEKLQNLGAKVTGSVSKKTDLIV
                     AGSDAGSKLTKAQDLGITIQDEDWLLNL"
     gene            16440..17459
                     /locus_tag="VBC94_000748"
     CDS             16440..17459
                     /locus_tag="VBC94_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO6979034.1"
                     /translation="MKKQKKARLIYNPTSGQEIMKKNVAEVLDILEGFGYETSAFQTT
                     PTKNSARDEAARAAQAGFDLIVAAGGDGTINEVVNGIAPLKRRPKMAIIPTGTTNDFA
                     RALKIPRGNPIEATKLIGKNQIVKMDIGQAQEDNYFINIAAAGSLTELTYSVPSQLKT
                     TFGYLAYLAKGVELLPRVRKVPVKITHDKGEFIGDASMIFVAITNSVGGFEQIAPDAK
                     LDDGKFTLILVKTANLIEIMHLIRLVLAGGKHINDKRVEYIKTSYLTIEPLSDERMMI
                     NLDGEYGGDAPITLANLKNHIRFFANTDEISDDALVLDKDELAIEAIAQKFANEVNDL
                     EGLGD"
     gene            17463..19763
                     /gene="pulA"
                     /locus_tag="VBC94_000749"
     CDS             17463..19763
                     /gene="pulA"
                     /locus_tag="VBC94_000749"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEO6979035.1"
                     /translation="MDNTVIVHYHSRKKNYFNLSLWQWRDGSEGQDAHFSRFDSFGAV
                     AILKYPLPYFLSHYYVIVKDKNWHHKTIDYRIDCTKGGARKEVWIVDGDDNLYYSRQA
                     AVSSHHYSRRQPHAYDMAINSRAFDKKWGFDGWLGFEYSETKTAFRLWAPTAERVELI
                     LYHSTDETASVSKVLSMKRGTAVNYKNHKENTHGVWFTELEGNYNYQAYTYRVYYRRR
                     TFKITRDPYSIATTANGKRSIVIAPEALTPEGFKISHGKEAKWRLENPNQAVIYEMHV
                     RDFSISETSGVKTDYQGKFKGLHQKDTVNQHGDKTTFDYVQDLGVNYIQLQPIFDHHQ
                     TFDDDGHYAYNWGYDPENYNVPEASFSSNPHEPATRILELKSAIQAYHDAGIGVIMDV
                     VYNHTFSSTDSAFQLTVPDYYYRMNHNGTFQNGSGCGNETASEKEMCRKYILDSVLYW
                     VKEYNIDGFRFDLMGLHDVETMNIIRNELNKIDPRILVYGEGWDMGAGLTPQNKAKKD
                     NAYQMPGIGFFNDDVRDAVKGAEIYGEFKKGLVSGNSTEDIVAKGILGSDELVSYIDP
                     SQVLNYVEAHDNYNLNDLLWELHPNDNEKQHIYRVEVASAMNLLMQGMAFMQLGQEFL
                     RTKCYPTGDKGQLTQADKERAMNSYNAPDQVNQVNWDNVTFHKSTINFIRKIITLKTN
                     SPYFSYSSFEEIRKHVFVESAQYHSGFISFTVEEHKKIKIIFNIKGKRLQDPFIGDII
                     ISNHEEGKAIDSLDDELSVMILDITK"
     gene            19968..21836
                     /gene="glgB"
                     /locus_tag="VBC94_000750"
     CDS             19968..21836
                     /gene="glgB"
                     /locus_tag="VBC94_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEO6979036.1"
                     /translation="MSNKDELYTFGIGENFHLQNYLGVHSENGSFCFRVWAPNAENVQ
                     VIGDFTDWRNRPLQMNKIQAGVWEANSLDAREGDLYKYLVTRKGGQVVEKIDPMAVYM
                     ERRPGTASVIKVLRNKKWEDGLWMGRRKRLGFQKRPINIYEVHAGSWKKDDFGHPMTF
                     SQLKDYLIPYLVEMNYTHVEFMPLMAHPLDMSWGYQLMGYFAFEHTYGTPEEFQDFVE
                     ACHKNNIGVLVDWVPGHFIQNDDALAYFDGTATYEYQNHDRAHNYRWGALNFDLGKNQ
                     VQSFLISSALFWIEHYHIDGIRVDAVSNMLYLDYDEGPWEANQFGDNRNLEGYHFLRK
                     LNKVIKERHPNVMMIAEESTASTPITKDLESGGLGFDFKWNMGWMNDILRFYEEDPLY
                     RQYDFNLVTFSFMYIFNENFVLAFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNLYAYQ
                     MCHPGKKLLFMGSEFGQFLEWKYNDQLEWENLNDDMNQKMQRYTKQLNQFYKDHKCLW
                     RIDDSFDGLEIIDADNKSETVLSFIRKDDKGDLLLCVFNMTPVERPNFTIGVPQAGIY
                     EEVLNTEMEEFGGVWKNHNPVTKTQVATWKDYDHTLSFTLPALGASVWRIKRRLRK"
     gene            21878..23017
                     /locus_tag="VBC94_000751"
     CDS             21878..23017
                     /locus_tag="VBC94_000751"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019785022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEO6979037.1"
                     /translation="MKNEMLALIFVGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGINNVGVITQYQPLELNTHIGNGSSWGLDGIDSGVTVLQPYSATEGNRWFQGT
                     SHAIYQNIDYIDRINPEYVLILSGDHIYKMDYDDMLQTHKDNLASLTVAVLDVPLKEA
                     SRFGIMNTDSNDRIVEFEEKPEHPKSTKASMGIYIFDWKRLRTVLIDGEKNGIDMSDF
                     GKNVIPAYLESGERVYTYNFDGYWKDVGTIESLWEANMEYIGEDNKLHSRDRSWKIYS
                     KNLIAPPNFMTEDANVKDSLVVDGCFVAGNVEHSILSTNVQVKPNAIIKDSFVMSGAT
                     IGEGAKINRAIIGEDAVIGDGVVIDGSKEVEVIGYKEVAGVPNED"
     gene            23007..24140
                     /gene="glgD"
                     /locus_tag="VBC94_000752"
                     /pseudo
     CDS             23007..24140
                     /gene="glgD"
                     /locus_tag="VBC94_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687083.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
     gene            24137..25567
                     /gene="glgA"
                     /locus_tag="VBC94_000753"
                     /pseudo
     CDS             24137..25567
                     /gene="glgA"
                     /locus_tag="VBC94_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480692.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
BASE COUNT         8315 a         3819 c         5234 g         8247 t
ORIGIN      
        1 gttacttaga aaataaagaa aaatttttag tccccaatat tccatataaa aataaattaa
       61 ttttaaggga agaagataaa tatagttttg aagatgatga agaagagttt ggaaatgaac
      121 ttctaagtta caataagctt aagaatgaag ttttacctgt taatattaca acttctacta
      181 tattaaaacc gtttgaacag aagaaaattg tggaagattt taatccttat tctaatttag
      241 acaatttaga aataaaaaaa ataaggttga atggctccca aaaacaaaaa gtagaacagg
      301 aaaaaactaa atcgccaact cctcaaaaag aggctgtaaa agaacaaact gagcaaaaaa
      361 tatctgggaa gactcaaaac gtagaaaaga aatctgaaac tgtggcaact ccacaacaaa
      421 gttcaactgc gcaaacttct gtccaacagc cagctccggt tcaaccagtt gttcaagaat
      481 ctaaagcttc tcaagaggag attaatgcag cacatgatgc catttcagcg tataaatcaa
      541 cagtcaatat tgctaataca gccggtgtaa caactgcgga aatgacctcg ctcattaata
      601 ctcaaacttc taatctttct gatgttgaga aagctttagg gaataataag gttaataatg
      661 gtgcagtcaa tgtattgaga gaagatacat ctcgtcttga gaatatgatt tggaatcgtg
      721 cttaccaagc tattgaagaa ttcaacgtcg ctcgtaatac ttataataac caaatgaagt
      781 cagaaacagt tccagttgat aatgatattg aagctatttt agcaggttct caagctaaaa
      841 ttagccattt ggacaatcgt atcggagcgc gccacatgga tcaagctttt gtagctagtt
      901 tattagaagt tactgagatg agtaaatcaa tctcatcgcg tataaaagaa tagacactgc
      961 tatcaaggcg atcttaaact tttgtattga gctaacctaa aagatagaaa gagactaata
     1021 tgaaaaaaat aacaacttta atcttagcta gtagcttatt actagttgca acgacatcgg
     1081 ttaaagctga tgataacttt gaagtaccaa cgcgttatgt taaaatgagt gaaaaatcaa
     1141 aagcatttta tcaaagatta caagaaaaac aacgtaaaac acatactact gtgaagactt
     1201 ttaataattc agaaataagg catcaactac ctcttaaaca agaaaaggct aggaatgata
     1261 tctacaattt aggcattctt atttctcagg agtctaaagg gttcatccaa cgtattgata
     1321 atgcctattc tttggaaaat gtctcagata ttgttaatga agctcaggct ttgtataaac
     1381 gcaactatga tttatttgaa aaaatcaaat ctacacgtga taaggttcaa gtcttacttg
     1441 catcgcatca aggtaataca gatttaaaaa acttttatgc tgagttggac gatatgtatg
     1501 aacatgttta tctcaacgaa agtagagtgg aggcgataaa cagaaatatc caaaaatata
     1561 attagtttct aaactaacaa acattcctaa atataagaca ttaaacccta cttattgatt
     1621 agtgagtagg gttttactgt tttaaatagc tttctgctca gaatgtaagc cttgtcattt
     1681 caaaggaact atgttattat tcttaagtaa attaaatagg acatttgggg tgcgtaacag
     1741 ctgagattat acccattgaa cttgatacag ttagtactgt cgaagggaaa tgtgtgccat
     1801 tacaagtttt ttgtaatgga aaagggcgta gtgtcttctt aggagacagc tagctctagt
     1861 taatcagata ataaactccg tttgcttgca gatggagttt tttgtcgtca aaatttttta
     1921 taggttgttg ccccttatct gtcatagaga aggagttatg atgactaatc ataaaacaaa
     1981 aaaattaacg gttttagcaa ttatgattgc tttagatgtt tttctaacgc ccatttttag
     2041 aatagaaggc atggccttta tgtctagtgt ggttaatatt atcgctggag ttataatggg
     2101 accaatctat gcattagtta tggcacttgt aacggctttt attcgtattt taactcaagc
     2161 aggagtagca tctgttgcac ctttagctat tctaggagct ggtccaggag ctttcttagc
     2221 gggttggttt tatcggtttt atgcgaaaga ctggatgtct tgggtgggtg agtttatagg
     2281 aacagggata attggagcac ttttatcagc tccaattatg gtctggtttt ggacttggac
     2341 agcggatgga aatcacgaag tgttagctaa agcatctgca gctcaatcca tttttctttt
     2401 tatgccaagg tttatagggg cgactataat tggcggtgtt attgctttgt tgctacttca
     2461 gggattaaag cgggttcctg tatttatgac aatacaagca ttatttaaag gagaaaaagt
     2521 aaaatgaaat tgggtttaaa agatgtatta ttgattgctt tattagcggt tgttctgggc
     2581 gttgtttact ttggagcagg ctatatttca aatgcttttg taccttttgt aggtcctata
     2641 gcgcatgaag ttatttatgg tatttggttt gtggcaggtc caatggctct ttatatattg
     2701 aggaaaccag gaaccgctat tgtagcagaa ttgttagctg ctttgataga ggttttaatt
     2761 ggatcaattt atggaccaag tgtactcgtt attggtaccc ttcaaggttt agggtcagaa
     2821 ttaggcttta cgctgtttag gtatcataat tacaagttgc cagcttttat attgtctgct
     2881 attttgacat caatttttag ttttgcatgg agtttttatg ccaatggact tagtgcattt
     2941 tcattttcat ataacattct gatgcttata gtaagaactg tttcatctat tattttcttc
     3001 ttattaacaa agaatatatg tgaccaattg catagaagtg gtgtactgaa tgcttatggg
     3061 attcatgaag ggaagtagcc tatgctatct gttgaaaaat tagcttacac ccatggcgat
     3121 agtcattatt tatttgatga agtgactttt agtcttaacc caggagaacg aattttaatt
     3181 agtggctatt cagggtgtgg taagagtaca ttggctcttt tactatcagg gttaaaagaa
     3241 agtgggaaag gtcaagtttt attaaacggc tcattaatag aaccaagcga tgtgggtttt
     3301 ctttttcaaa atcctgattt gcaattttgt atggatactg tggcgcatga gttgtatttt
     3361 attttagaaa atttacaaat agaaccggag caaatgcaag atagaagtga gtttgtattg
     3421 gcacaagttg gtttaaaagg atttcagaat cgtttgattt atactttatc tcaaggtgaa
     3481 aaacagcgat tagcattggc gactattttt ttaaaatctc ctaaacttat tattttagat
     3541 gaagcttttg cgaatttgga tcaagagagt gccagtcaac tattacaatt agttcttaat
     3601 tatcaagcaa acaatcaatc aatgctgatt gtaatcgatc atttgattac ttattatcaa
     3661 gatataatgg atcactattt ttggttggaa aaatggttga taagggtcga ttttgattat
     3721 atgctcaatc gtttgaatgt cttcgaatta gaaaagaaaa gtcataatac aggtgacaaa
     3781 ctgttatcaa ttaaagattt ccaagttaaa ctttcaaaaa acaaattcat ctcttacctt
     3841 gattttgatt tagcaagtgg ggagaggttg tgcttagatg gaccaagtgg cgtggggaag
     3901 tctagtttct ttatgggatt gttagggctc tataggacta aaggaaagaa acaatttact
     3961 catagaaagc aaattcctat aagcttttta tttcaaaatc ccttagatca atttattttt
     4021 tcaacagttt atgacgaaat ttttcaggtt tgcaaggata gtaacaaagc gagggatatt
     4081 ttggaaacca taaatctttg ggataaaaaa cagttttcac catttcaact aagtcaaggc
     4141 caacagaggc gattagccat tggcagtatt ttagctagtg atagtaaact tctattatta
     4201 gatgaaccaa cttatggaca agatgcctat cacgctaata tgattacgac tcttcttttg
     4261 agttattgtc acaaaaatca ttgtggggtt atttttacta gccatgatcc tcacttaaaa
     4321 gagtgttttg caacacgttt cttggaggtg tcatcttgtt caatttgaaa aactatcatc
     4381 cagctatcac cttcctatgt ttattaatac caaccttttc gtttagtttc acattgaggc
     4441 taagaacgtc tttgttattt ctaattatag tagtaacact gcaatgcttc gtaaaggttt
     4501 ctttaaaaac ttgggcgaag gtcaatttga tctcatttgt gatgggactc tctttatttt
     4561 tagggacgta tttttggggg aaattacccc atcaattcgt cttagctagt ctagtggcct
     4621 gtcgtccgct tatttttatg aacgtgggtt tgttatttca tgcatcacat tctaattatg
     4681 attttataga atctctttat caaactttca aagtgcctag tcattttgca tatggtattt
     4741 ttgcggtctt taatctttta ccattaatta aattacaata ccaacgtaat cgactcgcat
     4801 tccgactgaa gaatcaggta acttgggcat tatcgcctag actaatattg tcagttttat
     4861 taaagacgat atattgggta gaacagttag agttagcaat gctttctaaa ggttttgaag
     4921 tgggtaaaga gcgaacacat gcttcaactt atcccgttcg ttttcgagat tatagtttac
     4981 ttggtatgag tattttattg gctataggaa tgatttttaa ataagtacaa ttagtttagt
     5041 aatacaattg tgcatttata tagagtgagc atgttctgct catgagatca taacaaagga
     5101 gattactatg ttaaaagatg agatgattgc taaaggaaca aaatatatac aatccatcta
     5161 tcaagatcca tttattcaag gaattgtaaa agggcgttta gaccatgacg taatctgcca
     5221 ttatttacag gcagataata tctaccttgg gaaatttgct gatatttatg ccctgtgctt
     5281 ggcaaaatct gataatttac gagataaaca atttttcctt gaacaagttg actttacatt
     5341 aaatcgggag ttagcagatg gtgaaggtcc acatcaagcc ttggcagctt atactaatcg
     5401 atcttatcag gatattatcg aaaaggctgt atggtacccg tcagcagatc attacattaa
     5461 acatatgtat tttcattttt acgaaaatgg tattgctggt gctttagctg caatgagccc
     5521 atgtccatgg atttaccatc aattggctaa aaaaataatt gaggagaacc agtttttgaa
     5581 tggtaaccct tttaataatt ggataacttt ttatgctaac gatacagttg aagagttaat
     5641 ggaaaactat tttcgtatga tggattatta tgcgcagaat ttgtcaaaag agaagcaagc
     5701 tgatttggta gatgcttttg tgaaatcttg tcagcatgag agacgttttt ttcaaatggc
     5761 aataaatcaa gaaaaatggg aggattaata tgacttattt agcaccagtt ttaactattg
     5821 caggaactga tcctagcggt ggagcaggga ttatggcaga tttaaagaca tttcaagctc
     5881 gtcggactta cgggatggct gttgtgacta gtgttgttgc tcagaacact tgtggggtta
     5941 gaggcgttca gcatatagaa acagctatca ttgatcaaca actagcttgt gtttatgatg
     6001 atattaagcc aaaggcggtc aaaacaggaa tgttagcgga gagggaaacg attagtttag
     6061 tcgcttcata tttaaaaaaa tatccgcaac cttatgtatt agatcctgtt atggtggcaa
     6121 ctagcggaca tcgtttaatt gattcagatg ccgtagaagc attaaaagaa gacttattgc
     6181 cattagcaac tattatcaca ccaaatttac cagaagctga ggttttagtg ggatatgact
     6241 tatcagatga agtaagtatt attaaagcag gttacgatat tcaaaaacaa tattctgtta
     6301 gaaatgttct tataaaggga ggacatctag atggtttggc taaagattat ttatttttgg
     6361 aaaaagaagg gttgataact cttagtaatc aacgtatcaa tactattcat actcatggga
     6421 ctgggtgtac atttgcagca gtcgttgcag cggagttagc taaagggcaa tcaattctaa
     6481 acgcggtaag taccgctaaa tcatttatta cttcagctat cgaaacagcg cctgagttgg
     6541 gtcttggaaa cggtcctgtc aatcatacta gctatcaagg agactaaaat gtatttagaa
     6601 caactaaaag aggtaaatcc tttaaccatt tgtatcacta ataatgtggt caaaaacttt
     6661 acagccaatg gtttattagc tttaggagct tctccggcta tgagcgagtg catagaggac
     6721 ttagaagact tgttaaaagt tgcagatgca ttattaatta atataggaac actaacaaaa
     6781 gaaagttggc aactttacca agaagcaatc aaaatagcaa ataaaaacca agttccagtt
     6841 gtattagatc ctgtagcggc aggagcaagt cgttttcgat tagaagtgtc acttgattta
     6901 ttgaagaatt atcctattag tttacttaga ggaaatggta gtgaaatcgc tgctttggtt
     6961 ggtgaaaaac aagcgtctaa aggggcagat gggggaaaag tggctgattt agaatcaatt
     7021 gctgtaaaag ccaatcaagt ttttgatgtg ccagtggttg tcacaggaga aactgatgct
     7081 atcgctgtta gaggagaagt tcgcttatta caaaatggta gtcctttaat gccacttgtt
     7141 acaggaactg gatgtctatt aggggcagta ttggctgctt ttattggtag ttctgacaga
     7201 agcgatgatc ttgcttgtct gacagaggcc atgactgttt ataatgttgc tggagaaatt
     7261 gcagagaaag tggcaaaagg taagggagtg ggaagtttcc aagttgcatt tttagatgcc
     7321 ctttctcaga tgaaatcaga aatgattatg gacaaatagg cggtgtcagt agatgaaaga
     7381 tacattgaaa ttatattttg tttgtgggac tgtggattgc tctaggaaga atatattgac
     7441 tgttgttgaa gaagccttac aagcaggtat aactctattt caatttagag aaaaaggttt
     7501 tacagcatta caaggtaagg aaaaaattgc tatggctaag caactccaaa tactttgtaa
     7561 gcaatatcaa gttcctttta ttattgatga tgacattgac ttagttgagt taattgatgc
     7621 tgatggtctt catattggac aaaatgattt acctgttgat gaggcacgga gacgtttacc
     7681 agataaaatc atcggtttat ctgtgagtac aatggctgaa tatcagaaat cacaattatc
     7741 tgttgttgat tacatcggaa ttggtccttt taatccgaca caatcaaaag cagatgctaa
     7801 gcctgctgtt ggcaatagga caacaaaggc agttagagaa attaatcaag atattccaat
     7861 cgttgcaata ggcggaatca ctagtgattt tgttcatgat attatagaat caggcgcaga
     7921 tgggattgct gttatatcgg ctatttcaaa agcaaatcat attgtagatg caaccagaca
     7981 attgcgctat gaagttgaaa aagctctggt taataggcaa aaacacagtg atgtaatcaa
     8041 ataaggctac atcgttaatt ataagaattt ttaagatatt atatcatcac acaatactgg
     8101 tatttgaaaa agaatttaaa ctatgataat atagtagagt tgaattctag ttaaagaaag
     8161 atttgaaagt atgcgtaaaa ttattattaa cggtggcaag caactgacgg gagaagttgc
     8221 tgtatcgggt gccaaaaata gtgtagttgc cttgattcct gccactattc ttgctgatga
     8281 tgttgtggtc ttagatggag ttccggctat ttctgatgtt gacagtttag ttgacattat
     8341 ggaaactatg ggagctaaga ttaagcgtta tggtgagacg cttgaaattg atccacgtgg
     8401 cgttaaagat attccaatgc cttatggcaa aatcaatagt cttcgtgcat cctattattt
     8461 ctatggaagc ttattggggc gttatggtca agcaacatta ggtttacctg gtggttgtga
     8521 tttaggcccc cgtccgattg atttacattt aaaagctttt gaagcgatgg gagctagtgt
     8581 ttcttatgaa ggtgattcaa tgcgtctagc aacaaacgga aagcctttgc aaggagcaaa
     8641 tatttatatg gatactgtca gtgttggtgc gacaattaac actatgatcg cagcagctaa
     8701 ggcaaatggt cgtacggtta ttgaaaatgc tgcgcgtgaa ccagaaatta ttgacgtggc
     8761 tactctttta aataatatgg gagctcatat tcgtggtgct gggactgatg ttattacaat
     8821 tgaaggtgtg aaatcgctcc atggcacgcg tcatcaagta attccagatc gtattgaagc
     8881 aggaacttat attgctatgg cagcagctat cggacgagga ataaaagtga ccaatgtgtt
     8941 atatgaacac cttgagagtt tcattgctaa gttagatgag atgggagttc gaatgacagt
     9001 tgaagaggat tcaatttttg tcgaagaaca agaaaagtta aaagctgtat ccataaaaac
     9061 ctcaccttac ccaggatttg ctacagattt acaacaacct ctaacccctt tattactaac
     9121 agcagaagga aacgggagtt tattagatac tatctatgag aaacgtgtta atcatgttcc
     9181 cgaattagca cgtatgggag ctaatatttc taccctaggg ggcaaaatag tttatagtgg
     9241 tcctaatcaa ttatctggtg cacctgtaaa agcgacagat ttgcgtgcag gcgctgcttt
     9301 agttatcgct ggcttaatgg cagagggacg aactgaaatt acgaatatcg agtttatttt
     9361 acgtggatat tctaatatca tcgagaaact aacatcattg ggtgcggata tccaattggt
     9421 tgaggaatag tatatataac aaagagttga gaatatttct tagctctttt cttcttgttt
     9481 tagattaagt cacttatagg tatttgaaat ctcttgtggt ataatattat catggatata
     9541 tggacaaatt taggacggtt tgcattcatt gaaacagaac atgttaacct acgtcctgtt
     9601 gcctacactg atagggaagc tttttggcga atagcttcaa aaaggacaaa tttacaattt
     9661 atttttcctg ttcaaaccag taagaaggaa agtgattttc ttttagttca ttcttttatg
     9721 aaagagccgt tgggagtttg ggcgattgaa gataaagtta gtcataaaat gtttggagtt
     9781 atccgttttg aaaacattga tttatctaaa aagacagctg aaattggcta ttttttgaaa
     9841 gaaagttctt ggggtcaagg cattatgact gaatgtctaa agacactttc tttttttgct
     9901 tttcaagagt ttgggatgga caaattgatc atagtaacac ataaagagaa tattgctagt
     9961 cagaaggttg ctttgaaggc gcatttcaaa caatctagga gttttaaagg aagcgaccgt
    10021 tatacacgcc gtataagaga ctatattgag tttcaattaa ctaggggtga ttatcgttat
    10081 gagtaaacat caagaaattt tggagtacct agaaaattta gctgttggta agagggttag
    10141 tgtacgcagt atttcaaatc atttaaaggt gagtgatggc actgcatacc gcgctataaa
    10201 agaggcagaa aataggggta ttgttgagac tagaccgcgt agtgggacag tacgtgttgc
    10261 tcaaaaagca aaggttaata ttgaaaaact gacttatgct gaaattgcaa ggattagtga
    10321 ctcacaagtt gttgcaggaa cagaggggct cagcaaggag tttagtaaat tttctatagg
    10381 tgcaatgact cacagaaata ttgaaaaata tttggtgcaa ggagggttat tgattgttgg
    10441 cgaccgtgat gaaatacaac atttagcctt acaacatcaa aacgctatct tagttacagg
    10501 tggctttaat gtttccccat ctgtttgtcg tttagcagat aaacttcaaa ttcccgtaat
    10561 ggtaacacac tatgatacat tcacagtatc aacgatgatt aatcacactt tgtcaaatgc
    10621 caaaattcgt actgatttaa agaccgttga acaagtctat cagtcgcaaa tggattacgg
    10681 tttcttagcg caggatgata ccgtaaaaga atttaatctt cttgttaaac aaactaaaaa
    10741 tgttcgtttt ccaatagtca accaagctaa tgttgtcgtt ggtgttgtta gtgtgcaaga
    10801 tattcttgga aaagataaag aagtaaaatt ggcaacagtc atgtcaaaaa atatcattgt
    10861 tgctaaacct cgaatgagtc tagctaatat cagtcaaaaa atgatttttg aagatttgaa
    10921 tatgatgcca gtagtatcgg atgactttga attattaggc gttattacaa gacgacaagc
    10981 tgtagaaaat ttatcgatgt cgcaagggac agctttatac acctatagtg atcaaatctt
    11041 gtctaattta caaattgaag atggtcactt ttcgttttta gtagaacccg ctatgattga
    11101 ccatacaggt agcttaacac aaggagtttt gacggagttc ttaaaagaaa tttgtattcg
    11161 tgtcttaact cgtaagcatc aacgcagtat tgttgttaaa caaatgacac tttatttctt
    11221 gcaacctgtt caaattgatg aaataataat ggtaacgcca acaattattt cagaaaaacg
    11281 gcgcgaagct acactcgatt tagaattaaa attagaaaat aaaattattg ctaaagctat
    11341 gatagcggta aaaattaatt agatataagg agaataaata gaaaatgatt acattaaagt
    11401 cagcacgcga aattgaagca atggatagag caggtgattt tttagccagc atccacattg
    11461 gacttcgtga tattatcaaa ccaggagttg atatgtggga agttgaagaa tacgttcgta
    11521 gacgttgtaa agaagaaaac gtattgccat tgcaaatcgg ggttgatggt gcagttatgg
    11581 attacccata tgctacttgt tgtgggctta atgatgaagt ggctcatgcc tttcctcgtc
    11641 attataccct aaaacaaggt gaccttttaa aagtagatat ggttttaagc gaacctttag
    11701 ataaatcaat tgttgatgtt tcatcgttaa actttgacaa cgtggctcaa atgaaaaagt
    11761 acacggaaac atatagtggt ggtttggcag actcatgttg ggcttatgct gtaggtgaag
    11821 tttcacaaga ggtgaaagac ttaatgtctg tgacaagaga agcaatgtat attgggattg
    11881 aaaaagcagt tatcggaaac cgtattggtg atattggagc tgctattcag gattatgcag
    11941 agagtcgtgg ttatggagtt gttcgtgatt tggttggtca cggtgttggt ccaacaatgc
    12001 atgaagagcc tatggtgcct aactatggta cagcaggtcg aggtctccgt cttcgtgagg
    12061 ggatggtttt aaccatcgaa ccaatgatta atactggtac ttgggaaatt gatacagaca
    12121 tgaaaactgg ttgggcacat aaaaccttag atggtggtct ttcttgtcaa tatgaacatc
    12181 aatttgttat cactaaagat ggtcctgtta ttttaacgag tcaaggagaa gaaagaactt
    12241 actaaaatga aattaaaaaa attctttgag gatttgctgg ctaaactaga atatagaccc
    12301 attcaagttt ttatgcgtca ttttcaaagt gcagagatgg acttatcagc catagcagtt
    12361 gcttattatc tattagtgac agcttttcca ttactagtta ttgctgctaa tatttttcct
    12421 tattttcata ttaatgtgtc ggatttattg tcacttatgc aaaaaaatct tccgaaaaat
    12481 atttatgaac ctgcctcacg cttagcagta gatgcttttt cgaaaccgtc taccggcatt
    12541 ttgggattcg cgtcattaac agctttttgg acaatgtcta aaagtttgac ctctcttcaa
    12601 aaagccatta ataaagcata cggtgttgac caacacagag actttgtgat tagtcgttta
    12661 gtgggtgtgg ggactgggct gatcatccta tttttactga cttttgtttt aattttttca
    12721 accttttcaa aaccagtttt acagataatt gtaaacatgt atgatttggg agatactcta
    12781 acggcgtggc ttttaaatct tgcacaacca gttacatttt tgactatatt tttaggtatt
    12841 ggaattttat actttatttt acctaacgca cgaattagaa aagtacgtta tgtcattcca
    12901 gggacattat tttcaacgtt tgttattggt ttttttagta atttaattag ccaatacgtc
    12961 ttaaataggg ttgaaaaaat ggtagatatt aaaacgtttg gttcggtcgt tatctttatc
    13021 ttaatgctat ggtttatttt cttagcccac attatgattt taggtgcaat actgaatgcc
    13081 agcgttcaag aaattgcgac aggaaaaatt gaatcaaggc gtggagatat tatgtctctt
    13141 atccaaaaat ccaaagaaga aaaataagaa aagacagctc ttgttagagt tgcctttttt
    13201 tgtctttaaa aataacaaat ttgctcaaga tgtagttgag taaaataatt aatatttgag
    13261 atacaaggct ttcaatggtg ttgattttat ttaaattgtg ctgaacgaat tgtccgataa
    13321 tagaaggaaa ttgatcaaca aaaataacag ctaatatcaa atctaatcct aatgtgccta
    13381 atctagcgat gacaaatgtt tgtaagcgtt gaaactgtcc aattttagtt tgtttaaaaa
    13441 caaatcgatc atttgtaaaa aaagcaaaaa gtatagctat tatgttagca actattgcag
    13501 agaatggagc gtcctgcgag aaattaaaaa tcccttgtcg aataactaaa taaagaaggg
    13561 atgttaatag tccgaacagt aaatataagc tgacttcatt ttgaatactt tttttcagta
    13621 acgattttaa tttaaccata tttttattat atcacggatg cttcaaagtg atgaggaaat
    13681 ctcccataag cttggcttta ctttttgttt gtgatatact tagtaagttg ttttacacaa
    13741 cccttgatgc ttagttccct ttcatcaagc atattacaaa cgttagaagt gttaactgtc
    13801 taaacgttaa aggagaaatt atgaacacat ttacaactcg cgattacgct cacatggcta
    13861 ttgtaacagc tatctatatc gttttaacca taacgccacc ttttaatgct attgcttatg
    13921 gtgcttatca attccgtgtt tcagaaatgc taaatttctt ggccttttat caccgaaaat
    13981 acctttttgc ggtaacgtta ggatgtatga tttctaatct ttatagtttt ggaatgattg
    14041 atgtctttgt aggtggtggt tcaactttac tttttgttta tcttggaacc attttattca
    14101 agcaatatca aaaagattat ctatttaatg gccttattaa caaagcattc tttttctttt
    14161 ctttcttctt tgctgcttca atgattacgg tagcagttga attgaaaatt gtagcaggat
    14221 tacctttatt attaacatgg ttaacgacag ctgttggtga attggcttca cttttggttg
    14281 gagcagtcct tgtggataag ttgagtagac acgtcgattt tacaaaataa atatattata
    14341 aagcgcgaga cattgatctc ggcttttttg tttggtttta tagttagcat agctagaaaa
    14401 tgctgcaact tttatgagaa atgctccaat ttttattaat atccttagtc tttgtgataa
    14461 aatagaaata tggaaaatcg tatgaatgaa ttagtatctt tgttgaatca gtacgcaaaa
    14521 gagtattata cacaagataa tcccacagtt tctgacagtc aatatgacca gttatatcga
    14581 gagttagtag aattagaaga gcaacatcca gaaaacattt tgccaaatag tcccacacat
    14641 cgagtgggtg gtttagtttt agaaggcttt gaaaaatacc aacatgaata tcctttgtat
    14701 tcattgcagg atgctttctc aaaagaagaa ttgatagcct ttgataaacg tgttaaagca
    14761 gagtttccaa ctgcttctta tatggcagag ctgaaaattg atggtctatc tgtctcgttg
    14821 acttatgtaa atggtatttt acaggttggg gcaacacgtg gtgatggcaa tattggagaa
    14881 aatattactg aaaatttaaa gcgtgttcat gatattcctc tacatttaga ccaaagttta
    14941 gatattactg ttcgcggaga gtgctacctt cccaaagaat cttttgaagc tattaacata
    15001 gaaaaacgtg caaatggtga gcaggaattt gctaatccta ggaatgcagc agcaggaacc
    15061 ttgcgccaat taaacacagg gattgttgcg aaacgtaaat tagcaacttt tctttatcaa
    15121 gaagctagcc ctacgcaaaa agaaacacaa gatgatgttt taaaagagct agaaagctat
    15181 ggcttttcag tcaatcatca tcgtttaatt agctcttcta tggagaaaat ttgggatttt
    15241 atccaaacta tagaaaaaga tagaatttcc ctcccttatg atatcgatgg tattgttatt
    15301 aaggttaata gcttagcaat gcaagaagag ttgggcttca ctgtaaaggc acctcgttgg
    15361 gcgattgcat ataaattccc tgctgaagaa aaggaagcag aaatattatc tgttgattgg
    15421 acagttgggc gaacaggagt tgtaacacca acagctaacc taactccagt tcaactagca
    15481 gggacaaccg ttagtagggc cactctgcat aatgtagatt atattgccga aaaagatatc
    15541 cgtattggag atactgttgt tgtatacaaa gcaggggata tcatccctgc agtcttaaat
    15601 gtcgttatga gtaagcgaaa ccagcaagaa gtcatgctta ttcctaaact atgtccttct
    15661 tgtggtagtg aattagttca ttttgaagat gaggtggctt tacgttgtat taatccgctg
    15721 tgccccaacc aaatcaaaga acgtttagct cattttgcta gccgggatgc tatgaatatt
    15781 acaggttttg gtccatcctt agtcgaaaaa ttatttgatg ctcatttaat agcagatgtt
    15841 gcagatattt atcgtttaag tatagaggat ttattaactt tagatggtat taaagagaaa
    15901 tctgcaacta agatttatca tgcaatccaa tcttcaaaag aaaattctgc tgaaaaactt
    15961 ctttttggcc taggtattcg tcatgtggga agcaaggcga gccgcctttt attggaggaa
    16021 tttggtaatt tgaggcaact aagtcaagca agtcaagaaa gtattgcctc aattgatgga
    16081 ctaggagggg ttattgccaa atcacttcat acgttctttg aaaaagaaga ggtagataaa
    16141 ctactagagg aattaacttc ctataacgtt aattttaatt atctaggaaa acgagttagt
    16201 acagatgcac aactttcagg tttaacagtc gttttaaccg gaaaacttga aaagatgact
    16261 cgtaatgaag caaaagagaa attacaaaac cttggtgcta aggtgacagg tagcgtttct
    16321 aaaaagactg atttaattgt tgcaggaagc gatgcaggaa gtaaactaac caaggctcaa
    16381 gatttaggga ttacaataca agatgaagac tggttactca acctttaagt gaggaagaaa
    16441 tgaagaaaca aaaaaaagcg cgtttaattt ataacccaac ttctggtcaa gaaattatga
    16501 aaaaaaatgt agcggaagtt cttgatatac ttgaaggatt tgggtatgaa acatcagcct
    16561 ttcaaacaac gccaactaaa aattctgcca gagatgaagc tgctagagct gctcaagctg
    16621 gttttgattt gattgttgca gcaggtggag atggtactat taatgaagtt gttaatggta
    16681 ttgctccttt gaaacgtcgt cctaagatgg caattattcc aactggaaca actaatgatt
    16741 ttgctcgtgc cttaaaaata ccgcgaggta atcctattga agcaactaaa ttaattggta
    16801 aaaatcaaat cgttaagatg gacattggtc aagctcaaga ggacaattat tttattaata
    16861 ttgcagctgc aggtagtttg acagagttaa cttatagtgt accaagtcaa ttgaaaacaa
    16921 cgtttggcta tcttgcttat ctagcaaaag gtgttgaatt attacctcga gttcgaaaag
    16981 taccagtcaa aattactcac gataagggtg aatttatcgg tgatgcttca atgatttttg
    17041 ttgccatcac aaattctgta ggagggtttg aacaaattgc acctgatgcc aaattggatg
    17101 atgggaaatt tacccttatt ttagtaaaaa ctgcaaatct tattgagatt atgcatctca
    17161 ttcgtttggt gctagcaggt ggaaaacata ttaatgataa acgagtagag tatattaaaa
    17221 cgtcttatct aactatcgaa cccctatcag acgagcgtat gatgattaac ttagatggag
    17281 aatatggagg agatgctccc attactctag caaaccttaa aaatcatatt aggttctttg
    17341 ctaatactga tgaaatttcg gatgatgctt tagttttaga taaagatgaa ttagcgatag
    17401 aagctattgc acagaagttt gctaatgagg taaatgattt agaaggatta ggagattgat
    17461 gaatggataa tactgtcatt gtacattacc atagccgaaa gaaaaattac tttaatttaa
    17521 gtttatggca gtggcgcgat ggctcagagg gtcaagatgc tcatttttca cggtttgaca
    17581 gtttcggtgc tgttgcgata ctaaaatatc ccttgccata ttttttaagc cattattatg
    17641 tgattgttaa ggataaaaat tggcatcata aaacaataga ttatcgcatt gattgtacta
    17701 aaggtggtgc acgtaaagaa gtttggattg tagatgggga tgacaatctg tattattctc
    17761 gtcaagcagc agttagtagc catcattata gtagacgcca acctcatgcc tacgatatgg
    17821 caatcaatag tagagcgttt gataaaaaat ggggatttga tggttggtta ggttttgaat
    17881 attcagaaac taaaacggct tttagattgt gggcccctac tgcggaacgt gtagaactga
    17941 ttttatatca ttcgacagat gaaacagcaa gtgtatcaaa agttctttct atgaaaagag
    18001 ggacagccgt caattataaa aatcataaag aaaacaccca tggcgtctgg tttactgaac
    18061 ttgaaggaaa ctataattac caagcatata cttatcgagt ctactataga cgacgtacct
    18121 ttaaaattac gagagaccct tattccattg caacgactgc aaatggtaaa aggtctattg
    18181 ttattgctcc agaagcatta actccggaag ggtttaaaat aagtcatgga aaagaagcta
    18241 agtggcggct cgaaaacccc aatcaagcag tcatttacga aatgcatgtg cgtgattttt
    18301 cgatatctga gacttcagga gttaagacag attatcaagg aaaatttaag ggattgcatc
    18361 aaaaagatac agttaatcag catggtgata aaacgacctt tgattatgtg caagacttag
    18421 gagttaacta tatccaactt caaccaattt tcgatcatca tcagacgttt gatgatgatg
    18481 gtcactatgc ttataattgg ggatatgatc ctgaaaacta caacgttcca gaagcaagct
    18541 tctctagtaa tccacatgaa cctgcaacgc gtattttaga attaaaatct gctatccaag
    18601 cgtaccatga tgcaggcatt ggtgttatta tggatgttgt ctataatcat actttttcat
    18661 caactgattc tgcatttcaa ctaacggttc cagattatta ttatcgaatg aatcacaatg
    18721 gaacatttca aaatggttca ggctgtggta atgaaacagc tagtgaaaaa gaaatgtgtc
    18781 gtaagtatat tcttgattca gttttgtatt gggtgaaaga atataatatc gatggttttc
    18841 gatttgattt gatgggacta catgatgtag aaacgatgaa cattataaga aatgagctaa
    18901 ataaaatcga ccctcgtatt ttggtttatg gagaaggatg ggacatggga gctggtctca
    18961 cgcctcaaaa taaagctaaa aaagataatg cttatcagat gcctggaatt ggctttttca
    19021 atgatgatgt aagagacgct gtcaaaggtg cggaaattta cggagaattt aaaaaaggct
    19081 tagtttctgg aaacagtaca gaagatattg ttgcaaaagg cattttagga agtgatgagt
    19141 tagtatcata cattgatcct agtcaagttt taaattatgt tgaagcacat gacaactaca
    19201 acttaaatga tttgctatgg gaattacatc ctaatgacaa cgagaaacaa catatttacc
    19261 gagttgaagt agctagtgct atgaatctat tgatgcaagg gatggctttc atgcaattag
    19321 gtcaggagtt tcttcgaaca aagtgttatc ctacaggtga taaaggtcaa cttactcaag
    19381 cagataaaga aagagctatg aattcttaca atgccccaga ccaagttaat caggtcaact
    19441 gggataatgt tacctttcat aagagtacta ttaattttat taggaaaata attactttga
    19501 agacaaactc tccttacttt tcttactcta gctttgaaga gattcgcaaa catgtttttg
    19561 tagagtctgc tcaataccat tcaggtttca taagttttac agtcgaagaa cataagaaaa
    19621 taaaaataat tttcaatatt aaaggaaaac gcttgcagga tcctttcata ggtgatataa
    19681 taatatcaaa tcatgaagaa ggaaaagcga tagattcatt agatgatgag ctttctgtaa
    19741 tgatccttga tatcacaaaa taacctgaaa acattcaggt tatttttgtg tatattaagg
    19801 aatttgattt gaatttgagt ttcttatatt ttagttaatt tttcggaaat atagagggtg
    19861 aaatattttc agatgcgtct gtatgaggaa aatgcattcc tctttttttg taccattgag
    19921 gaaatatttt tgtcttatat tgactagtag acctgtgagg tgagtagatg tccaataaag
    19981 atgagttata tacttttgga ataggtgaaa attttcatct ccagaattat ttaggcgtac
    20041 atagcgaaaa tggaagcttt tgtttcagag tttgggctcc taatgctgaa aatgttcaag
    20101 ttattggaga ttttacagat tggcgaaatc gacctctcca aatgaacaaa attcaagcag
    20161 gagtttggga agctaacagt ttggatgcgc gcgaaggtga tttatataaa tatttggtta
    20221 ctcgtaaagg tggtcaagta gttgagaaaa tcgatcctat ggcagtttat atggaaagac
    20281 gtcctggaac tgcaagtgtt attaaagttc ttagaaataa aaagtgggaa gatggtcttt
    20341 ggatgggaag gcgcaaacgt ctgggttttc agaagcgtcc aattaatatt tacgaagttc
    20401 atgctggttc atggaaaaaa gatgattttg gacatcctat gactttttct cagttaaaag
    20461 attacttaat cccctacctt gttgagatga attatactca tgttgaattt atgcccttaa
    20521 tggcacatcc gcttgatatg agttggggat atcaattaat ggggtatttt gcttttgaac
    20581 atacctatgg tactccagaa gaatttcaag actttgtaga agcatgtcat aaaaataata
    20641 taggagttct tgtagattgg gttccaggac actttattca gaatgacgat gctttagcct
    20701 atttcgatgg aactgctact tatgagtatc aaaatcatga tcgtgcgcat aactatcgat
    20761 ggggagctct gaactttgat ttaggaaaaa atcaagtaca atcctttttg atttcgagtg
    20821 ccttgttctg gattgaacat tatcatattg atggtattcg tgttgatgcg gttagtaata
    20881 tgttatatct cgattatgat gaaggaccat gggaagctaa ccaatttggg gataatcgta
    20941 atttggaagg atatcacttt ttaaggaaac ttaacaaagt aattaaagag cgtcatccta
    21001 atgttatgat gattgctgaa gagtcaacag cttctacccc aataactaaa gacttagagt
    21061 ctggaggatt gggttttgac tttaaatgga atatgggctg gatgaatgat atcctgcgtt
    21121 tttatgaaga agatcctctc tataggcaat atgattttaa tttagtgact tttagtttta
    21181 tgtatatatt caatgagaac tttgtactag ctttttcaca tgatgaagta gtacatggta
    21241 aaaagagtat gatgcataaa atgtggggtg accgttataa tcaatttgct ggtttacgta
    21301 atttatatgc atatcaaatg tgtcatcctg ggaaaaaact tctctttatg ggaagtgagt
    21361 ttggtcaatt tttagaatgg aaatataatg atcaactaga atgggaaaac ttaaatgatg
    21421 atatgaacca aaagatgcaa cgctatacta aacaattgaa tcaattttat aaagatcata
    21481 agtgcctatg gcgtattgat gattcctttg acgggctaga aattattgat gcggataata
    21541 aatctgaaac tgttttatct tttatacgaa aagatgataa aggcgatttg ttactttgtg
    21601 tctttaatat gactcctgta gaacgcccta actttactat tggtgttccg caagcgggta
    21661 tttatgaaga agttttaaat actgaaatgg aagagtttgg aggtgtttgg aaaaaccaca
    21721 atcctgtaac gaaaacacaa gtggctacat ggaaagatta tgatcatact ttaagtttta
    21781 cattaccagc ccttggggca agtgtttgga gaataaaacg tcggcttcgt aaataagtct
    21841 gcaatttagt aacctcattt tgaaaggaaa gaataatatg aaaaatgaaa tgttagcact
    21901 cattttcgtc ggaggacaag gcactcgact cggaaaattg actcagagca ttgcgaaacc
    21961 tgctgttcaa tttggaggtc gttaccgtat tattgacttt gcgctttcaa attgtgcgaa
    22021 ttcaggtatt aataatgttg gagttattac tcagtatcaa ccgctggaac tcaatactca
    22081 tattggaaac ggatctagct ggggactaga tggtatcgat tcgggagtaa ctgtattaca
    22141 gccatattct gcaacagaag gtaatcgctg gtttcaaggt accagccacg ctatctatca
    22201 aaatattgac tacattgatc gtattaatcc agaatatgta ctcattctct ctggggacca
    22261 catctataaa atggactatg atgacatgct acagacgcat aaagataatt tagcaagcct
    22321 aacagtcgct gttttagatg ttcctttaaa agaagcaagc cgttttggaa ttatgaatac
    22381 tgattcaaac gaccgtattg tcgaatttga agaaaaacct gaacatccta agtcaacaaa
    22441 agcttcaatg ggaatataca tttttgactg gaagagatta cggacagttt taattgatgg
    22501 tgaaaagaac ggcatagaca tgtcagactt tggtaaaaat gttataccag cataccttga
    22561 atcgggtgaa cgtgtttata cctataattt tgatgggtat tggaaagatg ttggtactat
    22621 tgaatccctt tgggaagcta atatggaata tattggagaa gataacaaat tacatagtcg
    22681 tgatcgttca tggaaaatct attctaaaaa tttgattgca ccacctaatt tcatgacaga
    22741 agatgcaaat gttaaagatt ctttagttgt agatggatgc tttgttgcag gaaatgttga
    22801 gcactccatt ttatcaacta atgtccaggt aaaaccaaat gctattatta aagactcctt
    22861 tgttatgagt ggcgcgacta tcggtgaagg tgcaaagata aatcgagcta ttatcggtga
    22921 ggatgctgtt attggtgatg gtgttgtgat tgatggaagt aaagaagtag aagttattgg
    22981 ttataaagaa gtagcggggg ttccaaatga agattgataa atatactgcg attttaggaa
    23041 atactgttag ttacccagat atgcacagtt tgacggataa tcgtccgctt gcttctctac
    23101 cttttgatgg taagtatcgt ttaattgatt ttcaattatc ctctttagct aatgcaggcg
    23161 ttagaagtat ttatggtatt tttcgtggga ataatgttcg ctctgttttt gaccatatta
    23221 gaagtggtcg tgagtggggc ttaggtacac tgttgagtca ctattcttta ggattctatg
    23281 atacagacga tgatactcgt acagtcaatg gggattatta cgattaaatt ttaacttact
    23341 tgaggcgttc gggtagcgac cagacgatat atatgagttg tgatatgctt tgtaatattg
    23401 acttaagtca agtaatccac ttgcataatg caaatggtgt gcaagccacc gtggtttata
    23461 aaaaaatgcc gagatcagct attgcagagg ctaatcaagt cttgaccatt gatgaatctg
    23521 atcatgtagt gagtctaggt aaagctgtag ataatgacga taaccaaaaa atgtctgctg
    23581 agatttatgt tatcgataca ccttggttaa tcgaaaaaat ggaagaggaa gctcaaaata
    23641 atgaaccacg taaactccgc ttcttacttc gtgatttaat tgttgaaagt aatgctttgg
    23701 cttttgaata cactggttat ttgtcaaaca tttcttctat taaatcctat tatgatgcta
    23761 acatggacat gttaacacca aataaatttt attcgctctt cttttcaaac caaaaagttt
    23821 atactaaggt gaaaaatgag gaagctactt attttgataa acaatcaaat gtctctaatt
    23881 cgcaactagc ctatggaagt attattaaag gttatttaga ccattctatc gtttctagaa
    23941 attgtctcct tgaaaaaggg actcgagtag tcaatagtat tatctttcca aaagtaaaaa
    24001 tcggagaagg ggcaaccatt gaaaatacta ttattgacaa atgcgttaag gttgcatccg
    24061 gagttacatt gaaaggtagt ttagataaac cgttagtgat tcctaaattt tcagagatta
    24121 atgaggatat tatacaatga aaataatgtt tgttgcggca gagggtgctc cctttgctaa
    24181 aacaggtggt ttgggagatg tgattggcgc actcccaaaa tcacttagta aaaaaggtca
    24241 tgatgtcgct gttgtcatgc catattatga tatggtagac caaaaatttg gagaccaaat
    24301 agaaaacctc atgtatttct atacagatgt tggctggcgt catcaatatg taggagttaa
    24361 gagactttct caagataatg ttacgttcta ctttattgat aatcaatatt atttttatag
    24421 aggtcatgtc tatggtgatt gggatgatgg ggagcgtttt gcttatttcc aattagctgc
    24481 tttagagttg atggagaaaa tagattttat tcccgatgtc ttacacgtcc atgattatca
    24541 tacagcaatg attcctttct tattgaaaga gaagtaccac tggattcagg cctataataa
    24601 tataagagca gtttttacta ttcacaatat tgaatttcaa gggcagtttg gtccagaaat
    24661 gttaggagat ttatttggag ttggcgctga gagatatgaa gatggaactc ttagatggaa
    24721 taattgtctt aattggatga aagcagcaat tctttactca gatagagtga caacggtatc
    24781 accaagctac gcaaatgaaa taaaaactcc ataatttggt aaaggtttag accaaattat
    24841 gcgtatggaa gcaggtaaat tatctggaat cgtaaatggt attgacagcg atttgttaaa
    24901 tccagaaaca gatgcttttt tgccatatca tttttcaaaa agcaacttag agggcaaaat
    24961 taaaaataag ttggctttac aggaaaattt gggcttgcct caagataaaa atgtaccatt
    25021 aattggtatt gtttcacgac ttaccgatca aaaaggattt gatattattg cttctgaatt
    25081 ggacaatatg cttcaacaag atattcaaat ggttatttta ggaacaggct atcaccattt
    25141 tgaagaaacg ttctcttatt ttgctagtcg ttaccctgaa aaactatctg ctaatattac
    25201 ttttgactta ggattagcgc aacagattta tgcggcttca gatatcttca tgatgccaag
    25261 tgcttttgaa ccttgtggtc tctcgcagat gatggctatg agatatggta gcttaccctt
    25321 ggtacatgaa gttggaggat taaaagatac tgtggtagcc ttcaatcaat ttgatggtag
    25381 tggtacaggt ttttcattta atcatttttc aggctattgg ttaatgcaaa ctctaaaact
    25441 agcattagaa gtttataatg attatcctga agcgtggaaa aagttacaat ggcaagctat
    25501 gtcaaaagat ttttcatggg atacagcatg tgttgcttac gaacaactgt atcagcaact
    25561 acaataatca ctttatttta gtgattagtt gataagctta tcgaatctct atgct
//
LOCUS       DATROQ010000020        24451 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.020, whole genome shotgun sequence.
ACCESSION   DATROQ010000020 DATROQ010000000
VERSION     DATROQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24451
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.020"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(14..694)
                     /locus_tag="VBC94_000754"
     CDS             complement(14..694)
                     /locus_tag="VBC94_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEO6979038.1"
                     /translation="MKEAKNFSQQLNILRAGVLGANDGIISVAGVVIGVASATHNLWI
                     IFLSAASAILAGAFSMAGGEYVSVSTQKDTEQAAVAREEKLLENNPELAKKSLVDIYL
                     AKGESHEHAQWLVDKAFSKNAIEHLVEEKYGIEFGEYTSPWHAAISSFIAFAIGSIFP
                     TITILLLPFSVRIVGTVIIVIVSLLSTGYVSAKLGQAPTVPAMRRNVMIGCLTMLATY
                     VIGQLFSI"
     gene            847..1875
                     /gene="queA"
                     /locus_tag="VBC94_000755"
     CDS             847..1875
                     /gene="queA"
                     /locus_tag="VBC94_000755"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEO6979039.1"
                     /translation="MNTNDFDFYLPEELIAQTPLEKRDASKLLVIDHKNKTMTDSHFD
                     HILDELKPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTEGDQWEVLAKPAKRL
                     RVGNRVSFGDGRLIATVTKELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     RDRYQTVYAKENGSAAAPTAGLHFTKELLEKIETKGVKLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSKEAADTLNAVKESGGRIVAVGTTSIRTLETIGSKFNGELKADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGHDFVLEAYNHAVEERYRFFS
                     FGDAMFVK"
     gene            2064..2399
                     /locus_tag="VBC94_000756"
     CDS             2064..2399
                     /locus_tag="VBC94_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6979040.1"
                     /translation="MTYLQYVILLVLWEEDHRHMKELSYALSLNSNTLTPVLKRMENR
                     GWIVREKPRDDKRQLIIHLSNKGIESQDAVVNAVQSCGYFFSEISPESYQKTVSALSS
                     LNQVIKSHL"
     gene            complement(2454..3518)
                     /locus_tag="VBC94_000757"
     CDS             complement(2454..3518)
                     /locus_tag="VBC94_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6979041.1"
                     /translation="MKQTKKEFILGLTIIITLTLLIMLIFLVMFNKSSNSKLKQTKDR
                     RILSTSTTRTSSSSHDQKIVEKSLEKKKMVMEQDMELMNAYRLYYDYAHLSLKEVVLA
                     YMSEYGIEKNSVAFSYKNLKTGQRDAMNDTQAMTAGSTYKLPLNMLVVDAVVKGKLSM
                     EERFDITETDYEYIGEHNNYVAAFSGAMSIDDMQKYSLVYSENTPAYALAERIGGMDS
                     AYSKFGRYGQSKGDIKNIQKNGNKVTTDYYIQVLDYLWKHRKKYNSLITYLEEAFPTD
                     YYRALIPSDVVVAQKPGYVREALNVGAIVKEEVPYIVAIYTAGLGGSTQEDSEINGVG
                     LYQLEQLCFVINQWHRVNMN"
     gene            complement(3619..4875)
                     /locus_tag="VBC94_000758"
     CDS             complement(3619..4875)
                     /locus_tag="VBC94_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEO6979042.1"
                     /translation="MEATFTTTGALIGLALAILLIIKKVHPAYSLILGALVGGLIGGG
                     DLVTIVNTMVLGAQGMMSSILRILTSGILAGALIKTGSAEKIAESIIKKLGQQRAITA
                     LAIATMIICAVGVFIDIAVITVAPIALAIGKKANLSKSSILLAMIGGGKAGNIISPNP
                     NTIAASEAFKVDLTSLMVQNIIPAIAALVVTIILAKIVSKKNNDISYDSEEQVGSDLP
                     AFLPAISGPLVVICLLALRPLFGITIDPLIALPLGGLISILATGYLKETVPFVEYGLS
                     KVVGVSILLIGTGTLSGIIKASNLQFDMIHLLEFLNMPTFILAPLSGIFMGAATASTT
                     SGTTIASQTFAETLIKSGVPAVSGAAMIHAGATVLDSLPHGSFFHATGGAVNMAIKER
                     MKLISYEALIGLTSTIVAVVYYCFFG"
     gene            complement(4900..6042)
                     /locus_tag="VBC94_000759"
     CDS             complement(4900..6042)
                     /locus_tag="VBC94_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEO6979043.1"
                     /translation="MKVVVAIDSLKGSLSSLEAGNAIKESINEVIPGADVEVHPLADG
                     GEGTVEALTLGMGGTIEIIPVKGPLGEKVHASYGIIPQRQLAIIEMAAAAGITLIATE
                     ERNPLHTTTYGVGEMINDAISKGCRHFIIGIGGSATNDGGAGMLQALGYALLDKDNQE
                     ISLGAQGLADLKSISTDKVIEELKECDFKIACDVTNPLCGAQGCSSIFGPQKGADEDM
                     ITKMDTWLSNYATLATSVSEKADATIEGTGAAGGLGFAFLAFTNATLEPGIDIILSEI
                     NIEKAISEADLVVTGEGRLDGQTVMGKAPIGVAKLAKKYGKKVVAFSGSVTEDAILCN
                     QHGIDAFFPIVRRLISLDEAMSKEVAYKNMKETATQVFRLINLYNH"
     gene            6208..7302
                     /locus_tag="VBC94_000760"
     CDS             6208..7302
                     /locus_tag="VBC94_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6979044.1"
                     /translation="MKLRKQLAQQIVTSIKDVCQQDINFINTKGIIFASTNPKRVGEF
                     HEIGLKVAQTGQMIEVTDQESYFGTQAGINIPFYYNCELLATIGISGNPNQVGKYALL
                     AQKMTRLILKEHELDYLDFGRKNEASIVLHHLVEGRELDYYYLNQFLNQYHLSEKTDY
                     RLLTFEINSQKQKLLLSQSEMSLLNFFDKLDTAIYTFNYPNQYWLLLSDQMFDYYYPN
                     ILSKFECEKGLYKVGIGQKSSLSLLKRSYETSILALKALKGQQKVNLVDDLDLELLLT
                     SIDSNIKQYVLNKALVNLSENDKILLNSYFKHNLSLKECSQELFIHKNTVQYRLNKIY
                     ESTQLNPRNFKDATLLYLALKSEDIYLHKL"
     gene            complement(7371..8798)
                     /locus_tag="VBC94_000761"
     CDS             complement(7371..8798)
                     /locus_tag="VBC94_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEO6979045.1"
                     /translation="MTIFPKHFIWGGAVAANQVEGAFRTDGKGLSVQDVLPNGGLGDF
                     TAKPTPDNLKLEAIDFYHNYKNDIKLFAEMGFKVFRTSIAWSRIFPNGDDSAPNEAGL
                     QFYDNLFDELLKYNIEPLVTLSHYETPLHLAKTYNGWADRRLIAFFEKFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGAIMADKSQLSPQELYQAIHHELVASARVTKLGRSI
                     NPNFKIGCMILAMPAYPMTSDPRDVLAARQFEQHNLLFSDIHVRGKYPTYIQSYFKNN
                     GIEIKFEEGDEEVLAQNTVDFLSFSYYMSVTQAYDFENYQSGQGNILGGLTNPYLTTS
                     EWVWQIDPIGLRLVLNQYYERYQIPLFIVENGLGAKDQLIETLDGDYTVEDDYRIDYM
                     NQHLVQVAKAIEDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGTGSLQRY
                     KKKSFGWYQKVIKTNGQSLFEHHNR"
     gene            complement(8815..10683)
                     /locus_tag="VBC94_000762"
     CDS             complement(8815..10683)
                     /locus_tag="VBC94_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEO6979046.1"
                     /translation="MTKYQETAKAILAAVGGEKNIQHVTHCVTRLRLVLDNDEIVNDQ
                     VIKTIPNVIGVMRKNDQYQIILGNDVNNYYNAFLALGHFENTTREFSSQKKSSIFEKL
                     IETIAGVIKPLIPALLGGGMLKVIGILLPMLGITSSSSQTVAFINFFGDAAYYFMPIM
                     IAYSAASRFKVTPVLAATVGGILLHPAFVTMVAEGKPLSFFGAPVTLASYGSSVIPIL
                     IMVFLMQYIERWINKIVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGKGLSSAML
                     SIYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVTTPDVLILPAMLASNLAQG
                     AASLAVAVKAKQKQTRQVAFAAGLSALLAGITEPALYGVTLKFKKPLYAAMISGGLVG
                     AYIGLVNIASYTFVVPSIIGLPQYINPQGGNNFSNAVIVAIATIILTFIITWFLGIDE
                     GENEKSSINAQEYTHIRSGLSKKETLYSPMVGNVLPLSKVPDETFSSKLLGEGLAITP
                     SVGEVYAPFDGEIISLFPTKHAIALKDDKGVEVLIHIGIDTVELNGEGFEQLVKVGDF
                     VKRGQLLLRMDIDFISSKGYSLISPVVVTNSIDQLEIIVKDEETMVTNEDDLLVIL"
     gene            complement(10676..11527)
                     /locus_tag="VBC94_000763"
     CDS             complement(10676..11527)
                     /locus_tag="VBC94_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO6979047.1"
                     /translation="MIIKRVLNHNAVISVTHQGLDVLLMGKGIAFKKRIGDRINSDAI
                     EKSFVLKNSDNMNRFTELFITVPEEVVACSERIINLGKIKLGKNLDEILYINLTDHIH
                     SAIERHEQRMVIQNPLRLEIQRYYPDEYSIGMKALELIKDELGICLTIDESAFIAMHF
                     VNAGLDNPFNEAHKITEIVSYIEQKVKIDFRTELDESSIDYYRFMTHTKLFAQRVLSG
                     MKYEDDDADLLLVVKKKYPREYKCVKEIGNNMAIQYQYQLNSSELLYLTVHVKRLVKN
                     LKEISDD"
     gene            complement(11865..12473)
                     /locus_tag="VBC94_000764"
     CDS             complement(11865..12473)
                     /locus_tag="VBC94_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEO6979048.1"
                     /translation="MAIILPDLPYAYDALEPHIDAETMTLHHDKHHATYVANANAALE
                     KHPEIGEDLEALLADVSQIPEDIRQAVINNGGGHLNHALFWELMSPEETQISQELSED
                     IDATFGSFEDFKAAFTAAATGRFGSGWAWLVVNAEGKLEVLSTANQDTPIMEGKKPIL
                     GLDVWEHAYYLNYRNVRPNYIKAFFEIINWNKVNELYQAAKA"
     gene            complement(12553..13590)
                     /locus_tag="VBC94_000765"
     CDS             complement(12553..13590)
                     /locus_tag="VBC94_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEO6979049.1"
                     /translation="MIAIEEIGRITPDNLGLVTVLAGEDLGQYAQMKEKLFQVIGFNK
                     DDLAYSYFDLSEEEYQNAELDLESLPFLSDYKVVIFDQFQDITTDKKTYLDEQAMKRL
                     EAYLQNPVDTTRLVICAPGKLDGKRRLVKLLKRDARVLEANTLKESDLKTYFQKYAHQ
                     EGLVFEAGVFDELLIKSNYDFSDTLTNIAFLKSYKTDGHISSNDVREAIPKSLQDNIF
                     DLTQDVLLGRIDLARDLVRDLRLQGEDEIKLIAIMLGQFRMFLQVKILASKGKSESQI
                     VSELSHYIGRKINPYQVKFAVRDSRNLPLAFLKEAIRILIETDYAIKRGTYDKDYLFD
                     LALLKIAHKKP"
     gene            complement(13644..14372)
                     /locus_tag="VBC94_000766"
     CDS             complement(13644..14372)
                     /locus_tag="VBC94_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6979050.1"
                     /translation="MFHREDFSKALLKKALQPGMRVMDIGCGSGELTRLAADIVGKEG
                     DVVGIDINEQLLWQARHDNGYANVSYQNADLAALSSFETKSFDAIIGRRVLMYQKNPK
                     KVIESLTPLLKQNGFYVFQESDAMCLELAKPSFVNMNKIQSLIWQTVEKEGGHIHIGS
                     QLEQLMLEAGLIVDHFVSENVIQTSQTGTDLSWLLNIMKERIQKYSDLQGLEDVNLQK
                     ELEENNDHFIRDCQFGIISHRVDE"
     gene            14548..15540
                     /locus_tag="VBC94_000767"
     CDS             14548..15540
                     /locus_tag="VBC94_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6979051.1"
                     /translation="MKNYRKLIVLLLLIFFAIFMGAYAYTHIVEKRSLTSNTIEKTLP
                     VVNQIKPQTIKEYQNYLTKVAKRNVLPVDIPQALNNEKVEITATDGMQTFTWNDKNNP
                     KQKVIFYVHGGSYIHQASELQYIFVNKLAKKLDAKVVFPIYPKAPTYNYSDAIPKIKK
                     LYQNTLASVTSHKQIILVGESAGGGLALGIADNLVTEHIKQPKEIILISPWLDIATNN
                     PKIEKVQKKDPLLKAWQLQQVAPYWANGKKNFKNPQVSPLYSSQFNKMAPISFFIGTH
                     DIFYPDNQLLHQKLAKENIKHHYIVGQKMNHVYPVLPIPEAETAFKQMLKIINH"
     gene            complement(15599..16543)
                     /locus_tag="VBC94_000768"
     CDS             complement(15599..16543)
                     /locus_tag="VBC94_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6979052.1"
                     /translation="MTSISDIAKKAGVAKSTVSRVINHHPHVSDETRQKVMALITELD
                     YIPNQLARDLSRGKTQKIGVVIPHTRHPYFTQLINGLLDAAKTTDYQLVVMPSDYNQE
                     LELSYLKQLKMEAIDALIFTSRAISLDIIETYAKYGRIVVCEKLQEYNHLSSAYLDRY
                     SSFLEAFSDMKLRGLEHLVLLFSRNNESSATYQSALLAYQEVYGQLSSPYMVVGNVHD
                     FNDGLNLSYQLVKEVSIDGILATSDEVAAGLIKGYEESRKKCPYIIGQECLLVGQLLK
                     LPTIDHKSYYLGKLAFKQALAEKISQEVLFSEFLSQDN"
     gene            complement(16554..17363)
                     /locus_tag="VBC94_000769"
     CDS             complement(16554..17363)
                     /locus_tag="VBC94_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6979053.1"
                     /translation="MTIKRIFCDMDGTLLNSEGQVSKSNATLIREAAIPVTLVSARAP
                     MEMKDAVDALQLGGVQVAFNGGLIYRIGDNNQVLPIHTQIIKKSTVKQLLRGIRFHFP
                     QVSLSYYDLNNWYCDKIDEGIRYEHSLTQQCPTFIHNEDQFLEGHTNTFKIMMITFDE
                     ANMLELEKYLQSLELPEITIQRSGKAYLEITHLLAKKSKGIAYILQKEQLAREETAAF
                     GDGHNDLPMLEMVGYPIVIDNAFDDIKAIAYQLTKSNDEDGVGYGIQKFLK"
     gene            complement(17489..19726)
                     /locus_tag="VBC94_000770"
     CDS             complement(17489..19726)
                     /locus_tag="VBC94_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEO6979054.1"
                     /translation="MLHLTKYFPLKPIYLALLVFQIYLLVFSWTMLGCAFLLFTFIFL
                     IYQYDRETIFKTITIVIFFLFYFLWQNHNMNVQYQRVPNHISQIKVRIDTISINGDVL
                     SFQADASGNTYQAFYTLKNKSEKDYFQNLDNNIMIIADIKLEEAEERRHFNGFDYRQY
                     LKRHGIYRIAKVTKIKQIRLFQHRSFFDLMFKWRRSAIVISQTFPNPMRHYMSGLLFG
                     YLDKTFDDMSDLYSSLGIIHLFALSGMQVGFFLGIFRYICLRIGLRLDHVWLLQIPFS
                     LIYAGLTGFSISVVRALIQSLLSHSGVKKDENFALCLLICLISLPHSLLTTGGVLSFA
                     YAFILTMTSFDHFSSIKKVAIESLTVSVGILPILTYYFSGFQPISIILTALLSFAFDI
                     IFLPLLTVIFVLSPFVKLSCINSLFEILEVLLKWTGQLFPRPLIFGKPSLFLLIVMII
                     ILGLLYDYYHSKCFRYCSLLIIFTLFFITKNPITNEVAILDVGQGDSILVRDWLGKTI
                     LIDTGGRVRFEQPEEWKQKVNQSNAERTLIPYLKSRGISKIDNLVITHTDTDHMGDME
                     VISKHFKVARLITSSGSLTNSQYVKRLSKIGVAVKSIEAGDKLSVMGSYLQVLYPWHK
                     GDGKNNDSIVLYGHLLGKGFLFTGDLEEEGEKQLLEAYPHLSVDILKAGHHGSKGSSS
                     LSFLKKLSPSVVLVSAGKNNRYQHPHQETLQRFQKIKSKIFRTDQSGTIRLTGWWKWH
                     IQTVR"
     gene            complement(19710..20363)
                     /locus_tag="VBC94_000771"
     CDS             complement(19710..20363)
                     /locus_tag="VBC94_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEO6979055.1"
                     /translation="MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDDLN
                     INLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGAVNHPGVYSLPSQSRVTDAIKRA
                     GGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
                     ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTLD"
     gene            complement(20463..21203)
                     /locus_tag="VBC94_000772"
     CDS             complement(20463..21203)
                     /locus_tag="VBC94_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEO6979056.1"
                     /translation="MFYTYLRTLVMFLIWVANGNAHYHNEDKMLKDDENYILVAPHRT
                     FWDPVYMAFAARPKQFVFMAKKELFTNRLFGWWIKMCGAFPIDREKPGQDAIRYPVKM
                     LKNSNRSLVMFPSGSRHSKDVKGGVAVIAKMAKVRIMPAAYRGPMVFKNLLKGHRVDM
                     NFGNPIDVSDIKRMDAEGIAEVSRRIQEEFDRLDRENETYDDGKKLNPLTYIYRLPLA
                     IIAIVLLVLTLIFSYLASFVWDPQKHLK"
     gene            21338..22102
                     /locus_tag="VBC94_000773"
     CDS             21338..22102
                     /locus_tag="VBC94_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEO6979057.1"
                     /translation="MIDTILKENERIDQLFSTDVKIIQNKEVFSYSIDSVLLSRFPKL
                     PSRGLIVDLCSGNGAVGLFASTKTSATIIEIELQERLADMAKRSIKLNKLEKQVTMIN
                     DDLKNLLDHVQRSNVDLMLCNPPYFKASETSKKNLSPHYLLARHEITTNLREICQIAQ
                     HALKTKGRIAMVHRPDRFLEIIDTMHQFNLAPKRIQFVYPKLGKDANMLLIEAIKDGS
                     TEGMKILPPLVVHQDNGDYTETIFDIYFGENGKSHD"
     gene            22095..22361
                     /locus_tag="VBC94_000774"
     CDS             22095..22361
                     /locus_tag="VBC94_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO6979058.1"
                     /translation="MIKVPAYMYVLECSDGTLYTGYTTDVKRRLNTHNTGKGAKYTRA
                     RLPVKLLYSEAFNSKQEAMRAEALFKQKTRKAKLTYIKQHKNEQ"
     gene            complement(22544..24130)
                     /locus_tag="VBC94_000775"
     CDS             complement(22544..24130)
                     /locus_tag="VBC94_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6979059.1"
                     /translation="MKFTELNLSQDILSAVEKAGFVEPSPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIHTEDNTIQALIIAPTRELAVQSQEELFRFGRDKGVKVRS
                     VYGGSSIEKQIKALRSGAHVVVGTPGRLLDLIKRKALKLNHIETLILDEADEMLNMGF
                     LEDIEAIISRVPEARQTLLFSATMPDPIKRIGVKFMKDPEHVKIKATELTNVNVDQYY
                     VRVKENEKFDTMTRLMDVDQPELSIVFGRTKRRVDELTRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDHIDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVSPNEMGYLTIIENLTKKRMTGMKPATASEAFQAKKKVALKRIARDFEDQ
                     ELVSKFDKFKADALELATQYTPEELALYVLSLTVQDPESLPEVEITREKPLPFKPSGG
                     GFKGKGGRGNGRGGDRRRNDRGDRRGNRDRDDRGGRRDFKRRDDKFKKDNRRQENKKP
                     HKNTSSEKQTGFVIRNKGDK"
BASE COUNT         8088 a         4726 c         3711 g         7926 t
ORIGIN      
        1 cacgattaat attttatatt gaaaataatt gtccaatgac ataggttgcc aacattgtca
       61 aacaaccaat catcacattt cgacgcatgg caggtactgt aggggcttgg cctaacttag
      121 cacttacata accagttgat aatagtgaaa caataacaat tattaccgtt cctacaattc
      181 gtaccgaaaa tggtaataac agaatagtta tagttggaaa aattgatccg atggcaaaag
      241 ctataaaact agatatagca gcatgccagg ggcttgtata ttcaccgaac tcaataccat
      301 atttttcttc aactaaatgc tcaatagcat tctttgaaaa agccttgtca actagccatt
      361 gagcatgttc gtgactttct ccttttgcta agtagatatc aactaaggat tttttggcaa
      421 gttctggatt attttctagt agtttctctt cacgcgcaac tgctgcttgt tcagtatctt
      481 tttgagtaga aacactaaca tactccccgc cagccataga aaatgcacca gctaaaatcg
      541 cggaagctgc tgataaaaaa ataatccaaa ggttatgagt tgcactcgca acaccaataa
      601 caacaccagc tacagagatg ataccatcat tagcaccaag aacaccagct cttaaaatat
      661 tcaattgttg ggaaaaattt tttgcttctt tcatcttctc tacttctcac tttctgacaa
      721 ttattatacc acttatttag aatgattaca attatttttt ataatcattc taaataagaa
      781 gattagttgg gtcattttta ccgaacgtga tataatagaa aagcttactt atagatagga
      841 gaaatcatga atacaaacga ttttgatttt tatttaccag aagaacttat tgcccaaaca
      901 ccacttgaaa aaagagacgc ttctaaacta ttagtcattg atcataagaa taagacgatg
      961 acagatagcc atttcgatca tatcttagat gaactcaagc ctggtgacgc tttagtaatg
     1021 aataacacac gtgttctgcc cgctcgcctt tatggtgaaa agccagatac acacggccat
     1081 gtagagttat tacttctcaa aaatacagag ggcgatcaat gggaagtgct tgctaaaccc
     1141 gctaaacgct tacgcgtagg gaacagagtt tcatttggtg atggtcggtt aatagcaaca
     1201 gtcacaaagg aattagaaca tggtgggcgt attgtagaat tctcatatga tggtattttt
     1261 ctagaagtcc ttgaaagttt gggcgaaatg ccacttccgc catatattca tgaaaaacta
     1321 gaagatcgtg accgctatca gactgtgtat gcaaaagaaa atggttctgc tgctgcacca
     1381 acagccggtc ttcattttac aaaagaacta ttggaaaaaa ttgaaacaaa aggtgtgaag
     1441 cttgtctatt tgacgcttca cgttggactt ggaactttcc gtcctgtttc agttgataat
     1501 cttgatgaac acgaaatgca ttctgagttt taccaattat ctaaagaagc tgctgatact
     1561 ctcaatgcag ttaaagaatc tggtggacgt attgtagctg ttggtacaac ttctattcgc
     1621 acattagaga caattggttc taaattcaat ggtgaattaa aagccgactc agggtggaca
     1681 aatattttta tcaagcctgg ttatcagttc aaggttgtag acgctttttc aaccaacttt
     1741 cacttaccaa agtctactct tgtcatgtta gtatcagcat ttgctggaca tgactttgta
     1801 ctagaagctt acaaccatgc agttgaagaa cgatatcgtt tcttcagttt tggtgatgct
     1861 atgtttgtta agtaagaata ggctttcaat taggaaaaag ttttatcgtt ttttaacacg
     1921 attgacatac tataacgcct ccaaaatgct acaatatgat aatgagtaat cgaagtattt
     1981 tagacgagcg attatgtttt acggttagtg aaacacatcg acttttcaat aaattttacc
     2041 aaaaagcttt ggatgaattt caattgactt atctccaata cgttatcttg ttagtccttt
     2101 gggaagagga tcatcggcat atgaaggaac ttagttatgc actatctctt aattcgaata
     2161 ccctaactcc agttttaaag cgtatggaaa atcgtgggtg gattgtacga gaaaaaccaa
     2221 gagatgacaa aaggcaactc attattcatc taagtaacaa aggtattgag tcacaagatg
     2281 ccgttgtcaa tgcggtgcaa tcgtgcgggt attttttctc agaaattagt cctgaatctt
     2341 atcaaaaaac tgtttctgcc ctttcttcac ttaatcaagt tataaaatca catttataaa
     2401 aacctcttag atagttaatc taagaggttt ttattatttt gatagattta tttctaattc
     2461 atattaacac gatgccattg attaataaca aaacataatt gttctagctg atatagtccc
     2521 actccattaa tttctgaatc ttcctgagta gagccaccta aacctgcagt ataaatagca
     2581 acgatataag gcacttcttc tttgacaatt gctccaacat tcaacgcctc acggacatac
     2641 cctggctttt gagcaacaac aacatcagat ggtatcaaag cacgataata atccgttgga
     2701 aaagcttctt ctaaataagt aattaaacta ttatactttt tacgatgttt ccagagatag
     2761 tctagcactt gaatatagta atctgttgtc actttattac cgtttttttg tatatttttg
     2821 atgtctcctt tagattggcc ataacgacca aactttgaat aagcactatc catgccacca
     2881 atacgctcag ctaaagcata ggcgggtgta ttttctgaat aaactaaaga gtatttttgc
     2941 atatcatcaa tagacattgc ccccgagaaa gcagcaacat aattattatg ttcaccaata
     3001 tattcatagt cagtctcggt aatatcaaag cgttcttcca tggacaactt gcccttgacc
     3061 actgcatcta ctaccaacat gttaagaggt aacttatagg tgctccctgc tgtcatagcc
     3121 tgagtatcat tcatagcgtc cctttgtcct gtttttaaat ttttataaga aaaagcaaca
     3181 ctattttttt caatgccata ttcagacata taggccagaa caacctcttt caaggacaaa
     3241 tgggcataat cataatagag cctataggca ttcatcaatt ccatatcttg ttccatgacc
     3301 attttctttt tttcaagaga cttttctaca attttttgat catgtgagct actggatgtc
     3361 cttgtggttg aagtagataa tattcttcta tcttttgtct gcttcaactt agagttactg
     3421 gacttattaa acattactaa aaaaatcagc atgattaata aagtaagggt aataataatg
     3481 gttaatccta aaataaattc tttctttgtt tgtttcacaa cacctctcct actaatttat
     3541 tatagtaaga aaattagaaa aaatcaatga gaattctatt caaacagtga taataaacat
     3601 cacgtattca tccaaatttt acccaaagaa acaatagtaa acgacagcaa ctattgtgct
     3661 agttaatcca atcaaagctt catagctaat tagtttcata cgttctttta tagccatatt
     3721 gacagctccg cctgtcgcat ggaagaagga tccatgaggt agcgaatcca gaacagtcgc
     3781 acctgcgtga atcatagctg cacccgaaac cgccggtacc cctgatttaa tcaaggtttc
     3841 agcaaacgtt tgtgaagcaa tagttgttcc agaagtcgtt gatgctgttg ctgctcccat
     3901 aaaaatacct gagagtgggg caaggataaa ggtcggcata tttaaaaatt caaggagatg
     3961 aatcatatca aattgtagat tagatgcttt aataatacct gaaagtgtac ctgttccaat
     4021 caaaagaatt gaaacgccaa caacttttga taaaccgtat tcaacaaacg gtaccgtttc
     4081 ttttaggtaa cctgttgcta aaatactaat tagaccaccc aacggcagtg caatcaaggg
     4141 atcaatagtg attccaaata aaggacgaag agctaataag cagataacca caagtggacc
     4201 agaaatagcc ggtaagaatg ctggcagatc agagcccact tgctcctcac tatcataaga
     4261 aatatcgtta tttttcttgg aaacaatctt tgctaatata atcgtaacta ctaaagctgc
     4321 aattgcagga ataatatttt gtaccattaa gcttgttaaa tcaaccttaa atgcttctga
     4381 agcagcaatt gtattaggat tgggtgaaat aatattccca gccttaccac caccaatcat
     4441 tgctaacagt atgctagatt tagaaagatt agctttttta cctattgcta atgcaatcgg
     4501 agcaactgta ataactgcaa tatcaataaa gacacctact gcacaaataa tcattgttgc
     4561 gatggctaaa gctgtgatgg ctcgctgttg tcctaatttt ttaatgatac tttctgcaat
     4621 tttttcagct gatccggttt taattaaagc acccgctaaa atccctgacg ttaaaatgcg
     4681 taatatggat gacatcatcc cttgcgcacc taataccata gtattaacaa ttgtaactaa
     4741 atcaccgcca ccaatcaaac caccaactaa tgctccaaga atgagactat aggcaggatg
     4801 aacttttttg ataatcagta aaatagcaag cgctaatcct attaaagccc ctgttgttgt
     4861 aaatgtagct tccataattt atcagaattc ctttcaatat taatgattat acaaattgat
     4921 taatctaaag acttgagtag cagtttcttt catattttta taagctacct ctttagacat
     4981 agcctcatct aagctgataa ggcgtcgtac gattggaaaa aaagcatcaa taccatgttg
     5041 attacaaagt atagcatctt cggtgacaga tccagagaat gccactactt tcttaccata
     5101 ttttttagct aattttgcta cgccaattgg agcttttccc atcaccgttt gcccatccaa
     5161 acgaccttca ccagtaacta ctaaatcagc ttcagaaatc gctttttcta tgttaatttc
     5221 tgatagaata atatcaatac caggttctaa ggtggcattt gtaaaggcta aaaaggcaaa
     5281 tccaagccca cccgctgcac ctgttccttc aatagttgca tctgcttttt cactaactga
     5341 agtagctaaa gtagcataat tacttaacca agtatccatt tttgtaatca tatcttcatc
     5401 agcacctttt tgcggtccaa aaatcgaact acaaccttga gcaccacata aaggatttgt
     5461 tacatcacat gcaattttaa aatcgcattc ttttaattcc tcaataacct tatcagtaga
     5521 gatagatttt aaatctgcca atccttgtgc tcccaaactg atttcttgat tatctttatc
     5581 taataatgca tatcccaaag cttgtagcat acctgcccca ccatcattag tagcactacc
     5641 tccaatacca ataataaagt gcctacagcc tttacttata gcatcattta tcatttctcc
     5701 aactccataa gtggttgtgt ggagaggatt tctctcttca gtagcaatta gggtaatacc
     5761 agcagctgca gccatttcaa taatagctaa ctgacgctgt ggaataatac cataactcgc
     5821 atgtactttt tctccaagag gccccttaac agggattatt tcgatcgtcc cccccatacc
     5881 tagcgtcaaa gcttcaacag ttccttctcc cccgtctgct aatggatgta cttccacatc
     5941 cgcccctggg attacttcat taattgactc ctttatagca ttgccagctt ctaaagaaga
     6001 aagactccct ttcaaagaat caatagctac tactaccttc atttgaaaac ctccttgaaa
     6061 atattttgtg aatctttgtt attttttcac tattttatat cactcaatta tagtaacaaa
     6121 atcaaataac aaaaaatata gactatagaa caaatgttag cgtttttatg atatgatatt
     6181 tgttatatat aacaaaaggt aagctgaatg aaattaagaa aacaactcgc acaacaaatc
     6241 gttacctcta tcaaagatgt ttgtcaacaa gatattaatt ttattaatac caaaggaatc
     6301 attttcgcta gtaccaatcc aaaacgtgtt ggagaattcc atgaaattgg tttaaaagta
     6361 gcacagacag gacaaatgat tgaagtaaca gatcaagaat cttattttgg gacacaagca
     6421 gggatcaata ttcctttcta ttataattgt gaattattag ctactattgg gattagtgga
     6481 aaccccaacc aagtaggaaa atatgcttta cttgctcaaa aaatgactcg actcatctta
     6541 aaagagcatg aattagatta tctggatttc gggcgaaaaa acgaagcttc tattgtctta
     6601 caccatcttg ttgaaggcag agagttagac tactattacc tcaatcaatt tttaaaccaa
     6661 taccacctat cagaaaaaac tgactatcgt ctactcactt ttgaaatcaa tagtcaaaaa
     6721 caaaaattac tcttaagtca atctgaaatg tcactcttga acttttttga taaacttgac
     6781 actgctattt atacctttaa ttatcccaat caatactggc ttttactttc tgaccagatg
     6841 tttgactatt attatccaaa tatcctatca aagtttgaat gcgaaaaggg tctctataaa
     6901 gttggtatcg gacaaaaatc atctttatca cttttaaaaa gatcatatga aacgtctatc
     6961 ttagccctga aagctttaaa aggtcaacaa aaggtcaacc tcgtcgacga ccttgatctt
     7021 gaactgttat taacaagtat tgattctaat attaaacaat atgtgctaaa taaagcgctt
     7081 gtaaacttat ctgaaaatga taagatcttg cttaactcat attttaagca taatctatca
     7141 ttaaaagaat gtagtcagga actttttatc cataaaaata ctgttcaata taggttaaat
     7201 aagatttatg aatcaacgca actcaatcca agaaacttta aggatgcaac tttattgtat
     7261 ctagcactta aatcagaaga tatttacctt cataaattat aataaaaagg agtaacctat
     7321 ggatactcct ttttatatta aattgaaaca gagactagtt gtttaacatg ttatctgtta
     7381 tgatgttcaa acaatgattg gccatttgtc ttaatcactt tttggtacca accaaatgac
     7441 ttcttcttgt aacgttgtag actccctgtt ccatcatcat ttcgatcaac atatattaga
     7501 ccataacgtt tacttagttg agctgtagac atggatacac aatcaatgca tccccaagat
     7561 gtatatccca ttatttcaac accatcttca atagctttag caacttgaac taaatgttga
     7621 ttcatataat caatacgata atcatcttcg acagtataat caccgtctag tgtttctatc
     7681 aattgatctt ttgctcccaa tccattttct acaataaaca acggaatctg ataacgttca
     7741 taatactgat ttaagactag gcgtagccct atgggatcga tttgccatac ccattctgaa
     7801 gttgttaaat aaggattagt caggcctcca aggatatttc cctgaccact ttgataattt
     7861 tcaaagtcat aagcttgtgt cacgctcata tagtaactaa acgataggaa atctactgta
     7921 ttttgtgcca atacttcctc atctccttct tcaaatttaa tttcaatacc attatttttg
     7981 aaataggact gaatataagt gggatattta cctctgacat gtatatctga aaatagcaga
     8041 ttatgttgtt caaattgtct tgcagccaga acatctcttg gatcagatgt cataggataa
     8101 gctggcatag ccagaatcat acagccaatt ttaaaattag ggttgataga acgccctaat
     8161 tttgtcactc tagccgatgc tactaattca tgatgaatag cttgataaag ctcctgagga
     8221 cttagttgtg atttgtcggc cataatagca ccactggtga aaggcatatg taaaatagaa
     8281 ttcacttcat taaaagttaa ccaatatttt accttatctt tataacgctc catgactgtt
     8341 tgagcaaatt tctcaaaaaa tgctatcaaa cgtctatcag cccaaccatt ataggtcttt
     8401 gcaagatgaa ggggtgtttc ataatgagat aaggtaacca aaggctcgat attatattta
     8461 agcaactcgt caaataaatt atcataaaat tgtaagccag cttcgtttgg agcactatca
     8521 tcaccatttg gaaaaattcg agaccaggca atagaggttc taaaaacttt aaagcccatc
     8581 tctgcaaaca gttttatatc atttttatag ttatgataaa aatcaattgc ttctagcttc
     8641 aaattgtctg gagtaggttt ggcggtaaaa tcgcctagac caccatttgg aagtacatct
     8701 tgtacagata aacctttgcc atctgttcta aatgcaccct ctacctgatt ggcagcaact
     8761 gcacctcccc atataaaatg tttaggaaaa atcgtcatat tatcatcctt ccttttaaag
     8821 aattacaagt aaatcatctt catttgtaac cattgtctct tcgtctttaa cgataatttc
     8881 aagttggtca attgaatttg taactacaac tggactaata agagaatacc cctttgaact
     8941 aataaaatca atatccattc gcaaaagtaa ctgtcctcgt ttaacaaaat cgccaacttt
     9001 aaccaattgt tcaaaacctt ctccatttag ttctacagta tctattccaa tatggattaa
     9061 cacttcaact cccttatcat cctttaaggc aatggcatgt tttgtaggaa ataaacttat
     9121 aatttcacca tcaaatggtg cataaacttc acccacacta ggagtgattg ctaaaccttc
     9181 acctagtaat ttagatgaaa atgtttcatc gggtacttta gataaaggta atacattgcc
     9241 taccataggt gaatacaatg tctctttttt tgataaacca cttctaatat gtgtgtactc
     9301 ttgagcatta atactacttt tttcgttttc tccttcatca attcccaaga accatgtaat
     9361 aatgaaagtc aaaataatag tagctattgc tacaataaca gcattactga aattattgcc
     9421 cccctgtgga ttgatgtatt gtggcaaacc aataatagat ggcactacaa atgtatagga
     9481 agcaatatta actaatccaa tataagctcc tactaaacca cctgatatca ttgcagcgta
     9541 taaaggcttc ttgaatttca gtgtgacacc ataaagtgca ggttctgtga taccagctag
     9601 tagtgctgat aaacctgctg caaaggccac ctgacgtgtc tgtttttgtt tagccttaac
     9661 tgcaacagca agggaagctg caccttgagc taaattagaa gctaacattg ctggtaaaat
     9721 caaaacatct ggtgtcgtaa cagaagcagc taaaaagatt ggagcaaatg cccagtgcat
     9781 tcctgtcata acaaccaatg gcataatagc accaagaata gctaaagcta accatggtgc
     9841 cacatggtaa atcgaaagca ttgcactaga caatccttta ccaataatta ctcccagtgg
     9901 ccccacgacc actaaggcta aaaagccaga aatgagaata atcaaagtcg gttgtaaaaa
     9961 gctcttcatt acactaggaa caattttatt aatccatctc tcaatgtatt gcataagaaa
    10021 aaccataatt aagattggaa taacagaaga accataacta gccagtgtaa ccggagctcc
    10081 aaagaaagat aatggttttc cttcagctac cattgtcaca aaagcagggt gtaagagaat
    10141 ccctcctact gtagcagcta atacaggtgt aactttaaat cgtgacgcag ctgaataagc
    10201 aatcattatt ggcataaaat aataagcggc atcaccaaaa aaattgataa aagctactgt
    10261 ttgagaacta gagcttgtta taccgagcat agggagcaaa atacctataa ccttgagcat
    10321 acctcctcct agaagagcag gtataagcgg cttaatgact cctgcaatcg tttcaattaa
    10381 tttctcaaat attgaactct ttttttgtga tgaaaattct ctggtagtat tttcaaaatg
    10441 acctaaggcc aaaaaagcat tataataatt attgacatca tttcccaaaa taatttggta
    10501 ttgatcattt ttacgcataa caccgataac attcggaata gttttaataa cctgatcgtt
    10561 gacaatttca tcattgtcta aaactaatcg taatcgtgtc acacagtggg ttacgtgttg
    10621 aatatttttt tctcccccta ctgctgctaa aatagcttta gcagtctctt ggtatttagt
    10681 catctgatat ctcctttaaa tttttaacta atcgtttaac atgcaccgtc agatatagga
    10741 gttcacttga attaagttga tattggtatt ggatagccat attgttgccg atctctttaa
    10801 cacatttata ttctctcgga tattttttct taactaccaa aagtaaatca gcatcatcat
    10861 cttcgtattt cattcctgat aaaacgcgtt gagcaaataa tttggtatgt gtcatgaaac
    10921 gataataatc aattgaactt tcatctagtt ctgttcgaaa atctattttt actttctgtt
    10981 caatgtaaga tacaatctca gtgattttat gcgcttcatt aaacggatta tccaatccag
    11041 catttacaaa gtgcatagca ataaaagctg actcatcaat tgttaaacaa atacccaatt
    11101 catcctttat aagttccaga gccttcatcc caatggagta ttcatcagga tagtaacgct
    11161 ggatttccaa ccttagtgga ttttggataa ccattctttg ttcatgtctt tcaatagccg
    11221 aatgaatatg atctgtcaaa ttgatataaa gaatttcatc taagttctta cccaatttga
    11281 ttttacctag gttgataatt cgttctgaac atgcaactac ttcttctgga acagtaataa
    11341 ataactccgt aaaacggttc atgttatcac tattttttaa aacaaatgac ttctcaatag
    11401 catcgctatt aatcctatca cctattcttt tcttaaatgc aattcctttc cccatcagta
    11461 acacatctaa tccttgatgt gttactgata tgactgcatt atgatttaat acgcgtttta
    11521 taatcattaa agtcctagtc cttcactcgc acaataaaaa atgacccatc caagacacaa
    11581 caaaaagtcg tcgttgaata ggtccctgct cacaaaatgt gataacatcc tttattatgc
    11641 aattttatct gttatcagct tgcttacctt aaataataac acctgaaaac gtcttaattt
    11701 tatcatttct tactaatctt gtcaagcgct ttcaataaac ttattcgctt aattgaagat
    11761 aataggactg ttctagttct atctgagttt ggatttctag acttttaaaa acattatcaa
    11821 cttttagaat aataaaaaaa cgagcctatc gactcgtttt tttattaagc tttagcagct
    11881 tggtaaagtt catttacttt attccagtta ataatttcaa agaaagcttt gatgtagtta
    11941 ggacgaacat tacgatagtt taagtaataa gcatgctccc atacatcaag tcctaaaata
    12001 ggtttcttac cttccataat tggagtatct tgattggcag ttgaaagcac ttcaagtttg
    12061 ccttcagcat taacaacaag ccaagcccaa cctgaaccaa aacgtcctgt tgctgctgct
    12121 gtgaaagcag ctttaaagtc ttcaaatgaa ccaaaagttg catcaatgtc ttcagataac
    12181 tcttgtgaaa tttgagtttc ttctggtgac atcaattccc agaaaagagc gtggttaaga
    12241 tgtccaccac cgttattgat gactgcctga cgaatatctt ctggaatttg agaaacatca
    12301 gctaagagcg cctctaagtc ttctccaatt tcaggatgtt tctcaagagc agcatttgca
    12361 ttagcaacat aagttgcatg gtgcttatca tgatgtagtg tcattgtctc agcatcaata
    12421 tgtggctcaa gcgcatcata tgcatagggt aaatctggta aaataattgc catgtcgaaa
    12481 tcctcttttc atctattgtt acccttattc tataggaatt tggaacagct ttcaactctt
    12541 ttgtttcttt aattagggct ttttatgagc aatttttaat aaggctaaat caaagagata
    12601 atccttatca taagtccctc ttttaatagc atagtcagtc tcgattaaaa tcctaatcgc
    12661 ttcttttaaa aaagcaaggg gaaggtttct agaatctctg acagcaaatt ttacttgata
    12721 agggttgatt ttacgtccta tgtaatggga tagttcagaa acaatttgac tctctgactt
    12781 tcctttacta gccaatattt taacttgcaa aaacatccga aactgtccta acatgattgc
    12841 aatcaatttg atttcgtctt cgccttgtag acgtaaatct cttactaaat cacgcgctaa
    12901 atctatccta ccaagtaaga catcttgagt caaatcaaat atattatctt gcaggctttt
    12961 agggatagcc tctctaacat cattagacga aatatgaccg tctgttttat atgactttaa
    13021 gaaagcgata ttagttaaag tatcactaaa atcatagtta gacttaatca ataactcatc
    13081 aaaaacacct gcttcaaaaa ctaatccttc ttggtgagca tatttttgaa aatacgtctt
    13141 taaatctgat tcttttaaag tattagcctc taaaacacga gcatcacgtt tcaataactt
    13201 tacaagtcga cgttttccat caagttttcc tggagcacaa ataacaagtc tcgtagtatc
    13261 tacaggattc tgcaagtagg cttccaagcg cttcattgct tgttcatcta aataagtttt
    13321 tttatcagtt gtaatatctt gaaactgatc aaaaataaca accttgtagt ctgataaaaa
    13381 aggtaaactt tctaagtcta attccgcatt ttgatattct tcttcactta aatcaaaata
    13441 agaataggct aaatcgtctt tattgaatcc aattacctga aatagcttct ctttcatctg
    13501 agcatattgc cctaaatctt ctcctgctaa aacagttact aaccctaaat tatctggggt
    13561 aattctccca atttcttcaa tcgcaatcat attccttatt ataccataag gaaacttttc
    13621 aaggtgtcaa ataatactta aaatcattca tcaactctat gagaaataat tccaaattga
    13681 caatctcgga taaaatggtc attattctct tctaattctt tttgtaaatt gacatcttct
    13741 aacccctgaa ggtcactata tttttgtatt ctttctttca tgatatttag tagccaagat
    13801 agatctgtac cagtttgtga cgtttgaatg acattttctg atacaaaatg gtcaacgatt
    13861 aagcctgctt ctaacattaa ttgctcaagt tgacttccaa tatgaatgtg tcctccttcc
    13921 ttctcaacag tttgccagat tagtgattgt attttattca tatttacaaa gctaggctta
    13981 gctagttcta aacacattgc atcactttct tgaaaaacat aaaaaccatt ttgctttaac
    14041 aaaggtgtta aactttctat tactttcttt ggattttttt gatacattaa tacacgacgt
    14101 ccaataatag catcaaaact ttttgtttca aaggaggaaa gtgctgctaa atctgcattt
    14161 tgataactaa cattagcata gccattatca tgtctagctt gccataaaag ttgctcatta
    14221 atatcaattc ctacaacatc accttcttta ccaacgatat cagcagccaa acgcgttaat
    14281 tccccactac cacaaccaat atccataact ctcataccgg gctgcaaagc tttttttaat
    14341 aatgcttttg aaaaatcttc tctgtgaaac ataataacac tctcctctct tctattttag
    14401 caaattaaag gttcatacgt actatcagca aaaaattata ataggttgaa attttatagc
    14461 attattttct tgttttcgta tctactttat tatagaataa caactaatca tttttagcat
    14521 gacgagctca attacaaaag gataatcatg aaaaactatc gaaaacttat tgtactacta
    14581 cttctaatct tttttgccat ttttatggga gcatatgctt acacgcatat tgttgaaaaa
    14641 agatccctaa ctagcaatac tattgaaaaa actctacctg tggtaaatca gattaagcct
    14701 caaaccatta aagaatacca aaattactta actaaggtag ctaaacgtaa tgttcttcct
    14761 gtagacattc ctcaggcatt aaataatgaa aaggtagaaa ttactgctac tgatggcatg
    14821 caaacattca cttggaatga taaaaataat cctaagcaaa aggttatctt ctatgttcat
    14881 ggaggatcat atatccatca agcttccgaa ttacaatata tttttgtcaa taaactagct
    14941 aaaaaattag atgcaaaagt tgtctttcct atttacccta aagctcctac atataattat
    15001 agtgatgcta tccccaaaat taaaaaatta taccaaaata cattagctag cgtcacatct
    15061 cacaaacaga ttatcctagt aggtgaaagt gcaggcggag gccttgcttt aggtattgct
    15121 gataaccttg tcacggagca tatcaaacaa ccaaaagaaa ttattttaat atcaccatgg
    15181 ttagatattg ctactaataa tccaaagatt gaaaaagttc aaaaaaaaga tccccttctt
    15241 aaagcttggc aattacaaca agtagctcct tactgggcaa atggaaaaaa gaattttaaa
    15301 aatcctcaag ttagtcctct ttacagtagc caatttaaca agatggctcc aatatcattt
    15361 tttataggaa cgcacgatat tttctacccc gataatcaat tattacatca aaaattagca
    15421 aaagaaaaca ttaaacacca ctatatcgtt ggtcaaaaaa tgaaccacgt ttatccagtc
    15481 ttaccgattc ctgaagcaga gactgctttc aaacaaatgc ttaaaattat caatcattaa
    15541 ataaaaaggt ttccaatcaa gtttagttgg aaaccttttt tctattactt ttgaatgact
    15601 aattatcttg agataaaaac tctgagaata aaacttcttg actaatcttt tcagctaaag
    15661 cctgtttaaa agctaatttc cctaaataat aggatttgtg gtcaattgtt gggagtttca
    15721 aaagttgccc aactaataaa cattcctgac caataatata aggacatttt ttcctacttt
    15781 cttcataacc ttttattagc cctgccgcaa cttcatcact tgtagctaat ataccatcta
    15841 tactaacttc ttttaccaat tgataagata aatttagtcc atcattaaag tcatgaacat
    15901 tcccaactac catatatgga gaggatagtt gcccatagac ctcttgataa gctaataatg
    15961 cggattgata ggtagcagag gattcattat tccttgaaaa taataaaact aagtgctcca
    16021 aacctcgtaa cttcatatca gaaaatgcct ctagaaatga agaatagcga tcaaggtaag
    16081 cagaggatag atgattatat tcctgcaatt tttcacaaac tactattcgt ccatattttg
    16141 cataagtttc aataatgtcg aggctaattg ctcttgaagt gaatattaaa gcatcaatag
    16201 cttccatctt gagctgtttc aagtaggaaa gttctaattc ttggttgtaa tccgaaggca
    16261 tcacaacaag ttgatagtcg gtagttttag cagcatctaa taggccattt attaattgtg
    16321 taaaataagg atggcgcgtg tggggaatga cgacacctat tttttgtgtt ttgcctcgac
    16381 ttaaatcccg cgctagttga tttggtatat aatccaattc ggtgataaga gccattactt
    16441 tttgtcgcgt ctcatcagaa acatgtgggt gatgattgat aacacgggaa accgttgatt
    16501 tagcaacacc tgccttttta gctatatcac taatacttgt cattataagg ctcctacttt
    16561 aaaaattttt gaatcccata tcccactccg tcttcatcat tagacttagt tagttgatat
    16621 gctatcgctt tgatatcatc aaaagcatta tctataacaa tagggtaacc taccatttca
    16681 agcataggca aatcattatg tccatctcca aatgcagccg tttcttctct agctaactgc
    16741 tctttttgaa ggatgtaagc aattccttta gattttttgg ctaagagatg tgtaatttca
    16801 agatacgctt tacctgaacg ttgaatagtg atttctggta actccaaact ttgaagatat
    16861 ttttctaatt ctagcatatt tgcttcatcg aaagtaatca tcataatttt aaaagtattt
    16921 gtgtgacctt ctaaaaactg atcctcatta tgaataaacg ttgggcattg ttgagttaaa
    16981 ctatgttcgt atcggattcc ttcatctatt ttatcacaat accagttatt aagatcgtaa
    17041 taagataaac taacttgagg aaaatggaac cgtattcctc ttagcaattg ttttactgtg
    17101 ctttttttaa tgatttgtgt atgaataggt agaacttgat tattatctcc tattctataa
    17161 atcaatcctc cattaaaagc aacttggact cctcctaatt gaagtgcatc tacggcatcc
    17221 ttcatttcca taggtgccct agcagacact aaagttactg gaatcgctgc ctcacgtatt
    17281 aaggtagcgt tacttttact cacttgtcct tcactattta atagtgtacc atccatgtca
    17341 caaaaaatac gtttgatagt cataaaaaat acctctcttc ttctgaaatc agtataaatg
    17401 ctggaacccg ttccatgtca agcttaaaat tactgaatcc ttgcgaaaaa tagattgtat
    17461 ttaacagata cgcaatcatt ttaataaatc aacgaactgt ctgaatatgc cacttccacc
    17521 atcctgttag cctaattgta cctgattgat ccgttcggaa aatcttgctt ttaatctttt
    17581 ggaacctttg taaagtctct tgatgaggat gctggtaacg attattttta ccagctgaca
    17641 ctagaaccac actaggagac aactttttca gaaaggatag acttgatgag cccttagaac
    17701 catgatgtcc tgctttaagg atatctactg ataaatgagg ataagcttct aataactgct
    17761 tttctccctc ttcctccaaa tcaccggtaa ataagaagcc ttttcctaat aaatgtccat
    17821 ataaaacaat tgaatcatta ttttttccat cacccttgtg ccatgggtaa agtacttgta
    17881 aataacttcc catgacagaa agtttatcac cagcttctat agattttaca gctactccta
    17941 tctttgataa acgcttaacg tactgcgaat tcgttaaaga acctgaactt gtaatcaaac
    18001 gtgcaacttt aaaatgcttt gagataactt ccatatcccc catatgatct gtatcggtat
    18061 gagttatcac taaattatct atcttgctaa tacctctgct tttcaagtaa ggaatgagcg
    18121 ttctctcagc attagactgg tttacttttt gtttccattc ttcaggctgt tcaaatctca
    18181 cccttccccc agtatcaatt aaaattgttt ttcctaacca atccctcact aaaatactat
    18241 ctccttgtcc aacatctaaa atcgcaacct cgttagtaat tggattctta gtgataaaaa
    18301 acaaggtaaa gataataaga agggagcaat aacgaaaaca tttagaatga taataatcat
    18361 aaagtaatcc caaaattata atcatgacta ttaaaagaaa aaggctgggc tttccaaaaa
    18421 taagtggtct tggaaacagt tgcccagtcc attttaataa tacttctagg atttcaaaca
    18481 aactattaat acaacttaat ttaacgaacg gtgataagac aaatataaca gttaataaag
    18541 gcaaaaatat aatatcaaat gcaaaagata aaagtgctgt taatataatt gatattggtt
    18601 gaaaacccga aaaatagtag gttagtatgg gaagaattcc tacagagact gtcaaagatt
    18661 cgatagctac tttttttata ctcgaaaaat gatcaaagga ggtcatcgta agtataaaag
    18721 cataagcaaa gctaagaact cctcccgtag tcaaaagtga gtgggggaga gagataagac
    18781 aaattaacaa gcagagagca aagttctcat ctttcttgac accgctatgt gataataaag
    18841 attgaataag tgccctaacg actgagatac taaagcctgt taaaccagca taaattagcg
    18901 agaatggtat ttgaagtaac caaacatggt ctagacgtaa gccaatacgt agacagatat
    18961 aacgaaaaat accgagaaaa aaacctactt gcatacctga caaagcaaat aaatgtataa
    19021 tacctagact actatataaa tcggacatgt catcaaaggt cttatctaga tatccaaaca
    19081 aaagccctga catatagtga cgcataggat ttggaaaagt ttgactaata acaattgcac
    19141 ttctacgcca cttaaacata agatcaaaga aagacctatg ttgaaataag cgtatctgtt
    19201 ttatctttgt cactttggcg atacgataaa ttccatgtct ttttaaatac tgacgataat
    19261 caaagccatt aaaatgcctt ctctcctctg cttcttcaag tttgatatct gcaattatca
    19321 ttatattatt atcaagattt tgaaaataat ctttctcact tttatttttt aatgtgtaaa
    19381 aagcttgata agtgttacct gaagcatctg cctggaatga taaaacatca ccattgatag
    19441 aaatagtatc aatacgcact ttaatctggc taatatgatt cggtactctt tgatattgga
    19501 cattcatatt gtgattttgc cataaaaaat aaaataagaa aaaaattact attgttattg
    19561 ttttaaaaat agtttcacga tcatattgat aaatcagaaa aataaaagta aataaaagaa
    19621 aggcacaacc aagcattgtc caagaaaaca ctagtaagta aatttggaag accaacaatg
    19681 ctaaataaat aggttttaga ggaaaatact tagtcaagtg taacatcttg acgtaatttt
    19741 tctagtgttt tctcaccaat acctgataca tttttgaggt catcaatagt tttaaaacca
    19801 ccttgactat cacgataatc taaaatatct tgagctcttt tagctccaac gcctgatatg
    19861 gtctgaagac ttgataaatc tgctttatta agattaattt tccccttact atttccctga
    19921 gtagcaatat tatttgcttt ctcctcttcc acaacagtta ttttttcacc tttttgagca
    19981 acatagataa ccgtctcatc ttgtaatttc tgtgctagat taacggattt actatcagcg
    20041 agattgctta gtccacctgc acgcttaatc gcatctgtta cacgactttg agaaggcaaa
    20101 ctataaacac ctggatgatt gactgcccct tttacgtcaa ccgttacttg attagatact
    20161 ttatctttga cgtgactaat cattggtacc tttttttctg ttatagttga tacttttttc
    20221 tctaaattga tatttaaatc gtcttcttta tgatgagtgc caaaatgatt taaacccaaa
    20281 attccaaaca ataagagtcc tacaataatt cctgttgtct cccatttatg cgatttaatt
    20341 ttttccaata ctatttcaaa cataaaaata ttcctccatt tacttattcg taactaaaga
    20401 gattttccta ttaaaaacaa aaaaaactat ttactaaaaa gtaaatagtt tccatgtaac
    20461 ccttatttta gatgtttttg tggatcccac acaaagcttg ctaaataact aaaaattaga
    20521 gttaggacaa gcaagacaat agctataatt gctaaaggta gacgataaat ataagtcaat
    20581 ggatttaatt tcttaccatc atcataagtt tcattttctc tatctaaacg atcaaattct
    20641 tcctgaatac gacgagatac ttctgcaatc ccttcggcat ccatacgctt aatatcagaa
    20701 acatcaatag ggttaccaaa attcatatca acacgatgcc cctttaatag atttttaaaa
    20761 accataggac ctctatatgc tgctggcata attctgactt ttgccatttt tgcaataaca
    20821 gctacgcctc ctttgacatc tttggagtgg cggcttccac tcggaaacat caccaaagaa
    20881 cggttactat ttttcagcat ctttacagga tatcgaatag cgtcttgccc tggcttttca
    20941 cgatcaatag ggaaagcacc gcacatttta atccaccaac caaacaaacg attcgtgaat
    21001 aattcttttt tggccataaa aacaaactgt ttgggtctag cagcaaaagc catgtacaca
    21061 ggatcccaaa atgttctgtg tggagctact aaaatataat tttcatcatc ttttaacatt
    21121 ttatcttcat tatgatagtg cgcattacca ttagccaccc aaatcaagaa cataactaaa
    21181 gtacgtaagt aagtatagaa catgtttctc ctctaccttt catcaagtaa cttctatttt
    21241 atcatgcttc ctttttttta gaaagaatta tcttaaaatc aacctccaca ctttcaaaac
    21301 tagtgctagg aagctgtaat tttgctataa taagcctatg atagatacaa ttttaaaaga
    21361 aaatgaacgc attgatcagc ttttttcaac tgatgtcaaa atcatccaaa acaaagaagt
    21421 tttttcctac tccatagata gtgttttatt gtctcggttc cctaaacttc cttctcgtgg
    21481 tcttatcgtt gacttatgca gtggtaatgg tgctgtcggt ttgtttgcca gcaccaaaac
    21541 aagtgcgaca attatagaga tcgaactaca agagcgcctt gcggatatgg ctaagcgttc
    21601 aattaagctc aataaattgg aaaaacaggt tactatgatt aatgatgatt taaaaaatct
    21661 tcttgatcat gttcaacgtt caaacgttga tttaatgctt tgcaatccac cttatttcaa
    21721 agcaagcgaa acctctaaaa aaaatctaag cccgcattat ctccttgcaa gacatgaaat
    21781 aacaacaaac cttagagaga tttgtcaaat tgctcaacat gctctaaaaa caaaaggacg
    21841 tatcgctatg gtacatcgtc cagaccgttt ccttgaaatt attgatacga tgcatcagtt
    21901 caatttagca cctaaacgta tccaatttgt ttatccaaaa ttaggaaagg atgctaatat
    21961 gcttttaatt gaagctatta aagatggctc gacagaaggt atgaaaatac ttcctcctct
    22021 tgttgttcat caagataatg gagactatac tgagactatt tttgatatat atttcgggga
    22081 aaacggaaaa tctcatgatt aaagttcctg cttatatgta tgttttagaa tgttcagatg
    22141 gaacacttta tacaggttat actacagatg tcaagcgccg tctcaataca cacaataccg
    22201 gaaaaggtgc taaatatact cgtgcgagat tgccagtaaa acttctttat tctgaagctt
    22261 ttaatagcaa acaagaagca atgagggcag aagcattatt taaacaaaag actcggaaag
    22321 caaaattgac ttatattaaa caacataaaa atgaacaata atacgataat atactaagtg
    22381 caatgagcct gtcctcgctt ctttaacttt taaatttcaa cctcacttta atcttttgtc
    22441 ttcattctta aagttataag gcaattttac ggatatcact atctacgctt tatcttaaaa
    22501 aataaaaaag gagtcgctca gcaactcctt tttcgtaatc ctcttattta tcgcctttgt
    22561 tgcgaataac aaatccagtt tgtttttcac ttgaagtgtt tttatgaggt tttttatttt
    22621 cttgacgacg attatctttc ttgaacttat catcacgacg tttaaaatca cgacgacctc
    22681 cacgatcatc acggtcacga tttccacgac gatcaccacg atcattgcgt cgacgatcgc
    22741 caccacggcc attacctcgt ccacctttac ctttaaagcc accacctgat ggtttaaatg
    22801 gtaatggttt ttcacgtgtg atttcaactt ctggtaacga ttctggatcc tgaactgtta
    22861 agcttaagac ataaagagca agttcttctg gtgtatattg agttgctaat tctaaagcat
    22921 cggctttaaa tttatcaaat ttagaaacta attcttgatc ctcaaagtca cgtgcaatac
    22981 gtttcaatgc aacttttttc ttagcctgaa atgcttcgct tgcagtagca ggcttcattc
    23041 cagtcatacg ttttttagtt aagttttcaa taattgtcaa atatcccatt tcgtttggtg
    23101 aaacaaaagt gatagattga ccagatttac cagcacgacc agtacgtcca atacggtgaa
    23161 cataactttc tggatcttgg ggaatatcat agttgtaaac atgtgtaacg cctgaaatat
    23221 ctaaaccacg cgcagcaaca tctgtggcaa caagaatatc aatatggtca tttttaaagt
    23281 ctctgataac acgaagacgt ttattttgat ctaagtctcc atgaattcct tctgctcgga
    23341 aaccacgtaa ttttagacca cgagttaatt catctacacg acgtttcgta cgtccaaaga
    23401 caattgacaa ttctggttga tcaacgtcca tcaaacgtgt catagtatcg aatttttcat
    23461 tttccttaac gcgaacataa tactgatcaa cattcacatt agttaattca gttgctttaa
    23521 ttttaacatg ctcaggatct ttcatgaatt taaccccgat acgtttgatt ggatcaggca
    23581 ttgtcgctga gaataaaaga gtttgacgtg cttctggtac acgactaatg attgcttcaa
    23641 tgtcctctaa aaatcccatg ttaagcattt cgtctgcttc atcaagaata agagtttcga
    23701 tgtgattaag tttaagtgct ttacgtttaa ttaaatcaag taatcgacct ggagttccaa
    23761 caacaacgtg tgctcctgaa cgtaatgcct tgatttgttt ttcaatactt gacccaccat
    23821 aaacagagcg gaccttaaca cctttatcgc gtccaaaacg gaacaattct tcttgtgatt
    23881 gtactgcaag ttcacgtgta ggagcaataa taagtgcttg aatagtatta tcttcagtat
    23941 gaattttatt taaagttggt aacccaaaag ctgcagtttt tcctgtacct gtctgcgctt
    24001 gaccaatgac atctttgcct tcaagagcaa gtgggatagt catttcttgg attggtgatg
    24061 gctctacaaa gcctgctttt tctaccgctg aaagaatatc ttgcgataag ttaagttctg
    24121 taaatttcaa atgtttctct tttctaaaag acagtgcgaa gctgccttat gaacctttat
    24181 tagttatctt ttctgacaac ctacttagtg taacacaatt aaaagtaaaa agatataaaa
    24241 taaggtcaat ctaataaata acacgctttc ataaattgct agttctatta gttgcttcag
    24301 aaaatagaaa aaaggcaata aaaaccctcc atctataaag aaggagggtt catagtttat
    24361 ctatctagaa cgcaacgtct gcttcacttg gaatctcctt agtgatatag tgacgaatct
    24421 ctttacttgt tagtacttct ttaagtactt t
//
LOCUS       DATROQ010000021        24332 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.021, whole genome shotgun sequence.
ACCESSION   DATROQ010000021 DATROQ010000000
VERSION     DATROQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24332
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.021"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(34..744)
                     /locus_tag="VBC94_000776"
     CDS             complement(34..744)
                     /locus_tag="VBC94_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6979060.1"
                     /translation="MNIKRHSIFLWLTTSILAILMAVGMTIYFNTYQASPLARRIAKT
                     ATNHKNYLLFRSKGKVKANIIFYQGALVEEEAYSQLARDLADKGYNTYILKTPLNLPV
                     LSPYKAKTIINQNHLTNVYLAGHSLGGVVASQNAKVAPVRGLILLASYPSSKSDLSHK
                     KLRVLSITASNDHILNWEKYEEAKKRLPNSSTFRTIVGGNHSRFGNYGHQKGDGKATL
                     SHKSSEKQLATFISNFIH"
     gene            complement(741..1439)
                     /locus_tag="VBC94_000777"
     CDS             complement(741..1439)
                     /locus_tag="VBC94_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001907086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6979061.1"
                     /translation="MPFIFRHSFFNKVLIFWYTIIMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDTFDNPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIINVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPSHYSVHPGHGMN
                     TTIGHEKNFNPFFQ"
     gene            1607..2371
                     /locus_tag="VBC94_000778"
     CDS             1607..2371
                     /locus_tag="VBC94_000778"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEO6979062.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGVLDYNEETAQAAVD
                     KLSPEDAVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGIAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIVHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            2479..4986
                     /locus_tag="VBC94_000779"
     CDS             2479..4986
                     /locus_tag="VBC94_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEO6979063.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLVKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEQLFLEGFELRTPRIDTVELSQVFYPCLEKYSLGALAESLNIEL
                     TDAHTAIADARATAQLFIKLKAKISSLPKEVLETILTFADNLLFESYLLIEEAYQEAD
                     FVNPKEYYFWQGLVLKKEKAVGKPKKLSSDFQVNMALLGMDARPKQIVFADLVKAHFN
                     DQTTTFLEAQSGLGKTYGYLLPLLDQSQKQQIIVSVPTKILQDQIMAKEIKHIQELFH
                     IPCHSIKGPRNYLKLDAFYKSLQVQDRNRLINRFKMQLLVWLTETTTGDLDEIKQKQR
                     LESYFDQLKHDGEVTQSSLFYDLDFWKRSYDKVAQSQLVIINHAYFLERVQDDKDFAK
                     GKVLVFDEAQKLVLGLENFSRGQLDISHQLQVIQKIIDSPIPLLQKRLLESISYELSH
                     AAELFYRHNSFEFSETWLKRLKNSINALEVVGLDDLQTFFTATYTNYWFETDKVNEKR
                     LTILRGAREDFLKFSEFLPPTKKTYMISATLQISPKVYLSDLLGGFSSISTEKIAHEK
                     NANQKVWIDTSMPNILDLSPEQYAYEIAKRLQDIMTLKQPTLVLLTSKQTMFMVSDYL
                     DKWEIKHLTQDKNGLAYNVKKRFDRGESNLLLGTGSFWEGVDFVHRDRLIEVITRLPF
                     DNPKDYFIQKLSQSLTKEGKNFFYDYSLPMTVLKLKQALGRTTRREEQKSAVIILDSR
                     LVIKSYGQTIMHSLGRDFEISKEKINKVLTEMAKFLI"
     gene            5072..6265
                     /locus_tag="VBC94_000780"
     CDS             5072..6265
                     /locus_tag="VBC94_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6979064.1"
                     /translation="MTYLSERVLNMEESVTLAAGAKARELRVQGRDILSLTLGEPDFA
                     TPKNIQQAAIEAITDGRASFYTPSSGLPELKSAINAYFERFYGYSLKPNQVVVGTGAK
                     FILYTFFMTVLNPGDEVIIPTPYWVSYADQIKMAEGKPVFVTAKEVNHFKVTVEQLEA
                     VRTDKTKVILLNSPSNPTGMIYKAEELEAIGNWAVEHDILILADDIYGRLVYNGNIFT
                     PISSLSESIRNQTIVINGVSKTYAMTGWRVGFAVGNHDIIAAMSKVVSQTTSNLTAVS
                     QYATIEALNGSQESFEKMRLAFEERLNIIYPLLCQVPGFEVVKPQGAFYLFPNVTKAM
                     EMKGYTDVTAFTDAILEEVGLALVTGAGFGAPENVRLSYATDLGTLKEAVRRLHVFMG
                     SNEIN"
     gene            6286..7632
                     /gene="asnS"
                     /locus_tag="VBC94_000781"
     CDS             6286..7632
                     /gene="asnS"
                     /locus_tag="VBC94_000781"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEO6979065.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEVIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYSTYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYIAEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIKP"
     gene            complement(7672..8229)
                     /locus_tag="VBC94_000782"
     CDS             complement(7672..8229)
                     /locus_tag="VBC94_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6979066.1"
                     /translation="MTKSPARLISFISIAIAINLIGANLALFLRLPIYLDTIGTLLIA
                     VILGPWYAASTAFLSALINWMTTDIFSLYYSPVAIVVAIITGILIKRNCKPSSLLWKS
                     LIISLPGTIIASVITVILFKGITSSGSSIIAQFLHGIGLDMTSSLILVQVGTDYIDRL
                     ISLILVFSTITLLKKHSPNLINQRF"
     gene            complement(8226..9209)
                     /locus_tag="VBC94_000783"
     CDS             complement(8226..9209)
                     /locus_tag="VBC94_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEO6979067.1"
                     /translation="MNKEKIIIDCDPGIDDTLALMYAIQHPKLEVVAITITAGNSPVE
                     LGLKNTFVTLELLNRRDISVYVGNNLPLQREFVSAQDTHGMDGLGENNFTLAQPIIFQ
                     EESADCFLANYFEHKNDTSIIALGPLTNIARALQTNPKLGKHCKRFVSMGGNFKSHGN
                     CSPVAEYNYWYDPHAAQYVFENLDKKIEMVGLDITRHIVLTPNHLSYMERINPDVSTF
                     IQKITKFYFDFHWQYEHIIGCVINDPLAIAYFVNENIATGFDSYTDVACHGIAMGQTI
                     VDQYHFYKKDANSKILTSVNTNLFWNDFMTVLLHAQNSVIIEDLEMLGLNK"
     regulatory      complement(9353..9397)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            complement(9455..9868)
                     /locus_tag="VBC94_000784"
     CDS             complement(9455..9868)
                     /locus_tag="VBC94_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEO6979068.1"
                     /translation="MLYEVTSSNTQGVDGKVYLSNGKIVETNHPLNHLPGFNPEELIA
                     LAWSTCLNATIKAILEQKGFKDLKSRVDVNCQLMKEKQVGKGFYFQVNAVASIEKLSL
                     SDSKLIVNKAHSRCPISKLISNAKTINLDTVKWSV"
     gene            10022..10912
                     /gene="rapZ"
                     /locus_tag="VBC94_000785"
     CDS             10022..10912
                     /gene="rapZ"
                     /locus_tag="VBC94_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEO6979069.1"
                     /translation="MSDEQIKLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPTLVPK
                     FLELAAQSGDTSKIAMVVDMRSRLFFREINSILDSLEINDNINFKILFLDATDTELVS
                     RYKETRRSHPLAADGRVLDGISLERELLAPLKSMSQNVVDTSELTPRQLRKVISEEFS
                     NQDSQSSFRIEVMSFGFKYGIPLDADLVFDVRFLPNPYYKPELRDKTGLDTEVYDYVM
                     SFDESDDFYDHLLALIKPILPGYQKEGKSVLTVAIGCTGGQHRSTAFAHRLSEDLKAD
                     WTVNESHRDKNKRKETVNRS"
     gene            10909..11883
                     /locus_tag="VBC94_000786"
     CDS             10909..11883
                     /locus_tag="VBC94_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEO6979070.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLRLEDVEITAVVTVADDGGSSGE
                     LRSVMQLTPPGDLRNVLVALSDMPKFYEQIFQYRFAEGDGDFAGHPLGNLIIAGVAEM
                     QGSTYNAMQSLTQFFHTTGKIYPSSEHPLTLHAVFKDGHEVVGESQIADYKGMIDHVY
                     VTNTYNEETPTASRKVVDAILESDMIVLGPGSLFTSILPNLVIPEIKQALLETRAEVA
                     YVCNIMTQRGETEHFTDADHVEVLKRHLGQDAIDTVLVNIEKVPESYMENNHFDEYLV
                     QVEHDFSGLRKHARRVISSNFLKLEKGGAFHHGDFVVEELMNLVGTHL"
     gene            11880..12791
                     /gene="whiA"
                     /locus_tag="VBC94_000787"
     CDS             11880..12791
                     /gene="whiA"
                     /locus_tag="VBC94_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEO6979071.1"
                     /translation="MSFTVKVKEELLGHKSENKMELSAIIKMSGSLGLANHGLNLSIT
                     TENAKIARHIYSMLEEHYHLQPEIKYHQKTNLRKNRVYTVFIEEKVDVILADLKLADA
                     FFGIETGIEHSILDNDENGRAYLRGAFLSTGTVREPDSGKYQLEIFSVYLDHAQDLAN
                     LMKKFMLDAKVIEHKHGAVTYLQKAEDIMDFLIVIDAMEARDAFEEIKMIRETRNDIN
                     RANNVETANIARTITASMKTINNIIKIMDTIGFDALPSDLRQVAQVRVAHPDYSIQQI
                     ADSLETPLSKSGVNHRLRKINKIADEL"
     gene            12806..14203
                     /locus_tag="VBC94_000788"
     CDS             12806..14203
                     /locus_tag="VBC94_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEO6979072.1"
                     /translation="MACTTILVGKKASYDGSTMIARTEDSVNGDFTPKKLKVMTSKDQ
                     PRHYKSVLSNFEVDLPDNPLPYTSVPDALGKDGIWGEAGINSKNVAMSATETITTNSR
                     VLGADPLVSDGIGEEDILTLVLPYIQSAREGVERLGAILEKYGTYESNGIAFSDTEEI
                     WWLETIGGHHWIARRVPDDVYVTNPNQLGIDHFEFNNCDDYMCSSDLKEFIEQYHLDL
                     TYSNEHFNPRYAFGSQRDKDRHYNTPRSWAMQRFLNPEIEQDPRSLFIPWCQKPYRKI
                     TVEDIKYVLSDHYQDSVYDPYGPEGDAVSRRAFRSVGINRTSQTSILQLRPNKSLETT
                     GVQWLSYGSMPFATMVPLFTQVETVPDYFSNTTKDASTDNFYWTNRLIAALADPHFYQ
                     HEADIESYIERTMAQGHAHINGIDREVAENKEIDFQQKNQEMSDYIQKESQELLNRIL
                     FDASNLMTNRFSMGD"
     gene            14345..15865
                     /locus_tag="VBC94_000789"
     CDS             14345..15865
                     /locus_tag="VBC94_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEO6979073.1"
                     /translation="MRKKFLLLISFVAMFAAWQLVQVKQVWADSKLKVVTTFYPVYEF
                     TKNVVGDKADVSMLIKAGTEPHDFEPSTKNIAAIQDSNAFVYMDDNMETWAPKVAKSV
                     KSKKVTTIKGTGDMLLTKGVEEEGEEHEGHGHEGHHHELDPHVWLSPERAISVVENIR
                     NKFVKAYPKDAASFNKNADAYIAKLKELDKEYKNGLSNAKQKSFVTQHAAFGYMALDY
                     GLNQVPIAGLTPDAEPSSKRLGELAKYIKKYNINYIYFEENASNKVAKTLADEVGVKT
                     AVLSPLEGLSKKEMAAGEDYFSVMRRNLKVLKKTTDVAGKEVAPEEDKTKTVETGYFK
                     TKDVKDRKLTDYSGNWQSVYPLLQDGTLDPVWDYKAKSKKDMTAAEYKKYYTAGYKTD
                     VESIKIDGKKHQMTFVRNGKSQTFTYKYAGYKILTYKKGNRGVRYLFEAKEKDAGQFK
                     YIQFSDHGIKPNKAEHFHIFWGSESQEKLFEEMENWPTYFPAKMSGREVAQDLMSH"
     gene            complement(15978..16238)
                     /locus_tag="VBC94_000790"
     CDS             complement(15978..16238)
                     /locus_tag="VBC94_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEO6979074.1"
                     /translation="MKKDIHPDYRPVVFLDTTTGYKFLSGSTKSTKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAQ"
     gene            complement(16347..17282)
                     /locus_tag="VBC94_000791"
     CDS             complement(16347..17282)
                     /locus_tag="VBC94_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEO6979075.1"
                     /translation="MIIFQQILDKIKEYDTIIIHRHMRPDPDALGSQIGLRDIIRHNF
                     PKKKVLATGFDEPTLAWIAKMDQVTDQDYQGALVIVTDTANTPRIDDERYKKGDFLIK
                     IDHHPNDEVYGDLSYVDTNASSASEIVTDFALSCDLLLSTSAARVLYNGIVGDTGRFL
                     YPATTSKTLKIASKLREFDFDFSAMARQMDSFPFKIAKLQGFIFEQLEIDKNGAACVT
                     LTQEDLKRFDVTDAETAAIVGIPGKIDIVESWAIFVEQSDGHYRVRLRSKSHIINEIA
                     KRHNGGGHPLASGANSYSLEENQAIYQEIQEVLSL"
     gene            17548..18570
                     /locus_tag="VBC94_000792"
     CDS             17548..18570
                     /locus_tag="VBC94_000792"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEO6979076.1"
                     /translation="MTQAVLKELAKAELHCHLDGSLSLPAIRKLANMADIILPNSDKE
                     LRKYVIAPAQTESLVDYLKTFEFIRPLLQTKEALRFAAYDVARQAALENVIYIEIRFA
                     PELSMDKGLTASDTVFAVLEGLADAQKEFNIVARALVCGMRQSSHKTTKEIIKHIVDL
                     APKGLVGFDFAGDEFSYPTDSLVDLIQEVKRSGYPMTLHAGECGCAKHIADSLNLGIK
                     RMGHVTALTGQRALIKRFVEEDAVAEMCLTSNLQTKAASSIQSFPYQELYDAGGKITI
                     NTDNRTVSDTNLTKEYSLFVTYFGTKIEDFLVFNQNAVKASFTSDSEKDTLLHKLQEN
                     YDSYLK"
     gene            18629..19072
                     /locus_tag="VBC94_000793"
     CDS             18629..19072
                     /locus_tag="VBC94_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEO6979077.1"
                     /translation="MALAKIVYASMTGNTEEIADIVADKLRDLGLDVEVEECTMVDAA
                     DFEDADIAIVATYTYGDGDLPDEIVDFYEDLAEVDLSGKVYGVVGSGDTFYDYFCKSV
                     DEFEAQFALTGAQKGADCVKVDLAAEDEDIENLEAFAEEIASKLN"
     gene            19149..19424
                     /locus_tag="VBC94_000794"
     CDS             19149..19424
                     /locus_tag="VBC94_000794"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEO6979078.1"
                     /translation="MELEEIRQEIDEIDQQLVSLLETRMGLILEVIAFKKKHRLPVLD
                     NNRENEVLNNVLKKVQNHQFDDVIRATFKDIMTESRVYQKENIVDGD"
     gene            19417..20613
                     /locus_tag="VBC94_000795"
     CDS             19417..20613
                     /locus_tag="VBC94_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEO6979079.1"
                     /translation="MIKRFDNYAQYVLVLGFSLLIGGVVGAITAVFGRVLLFLTAFRS
                     DYIAYLLPFLSIVGLFIVFVYQKFGGKSVKGMGLVFEVGHGNEETIPKRLVPLVILTT
                     WLTHLFGGSAGREGVAVQIGATVSHYFQKYCRLQNASQLFLVMGMAAGFAGLFQTPLA
                     ATFFAIEVLVVGRLMVSYILPSLIAALTANFVSHSLGLEKFSHSIATSMALTPDIILK
                     LLVLGLCFGLCGNLFAYLLAKAKLIASSRLLNPYKRIFTLGLLATFLLFIFHFGRYSG
                     LGTNLIEASFTNKNLYDYDWLLKLCLTVITLAAGYQGGEVTPLFAIGASLGVIIAPIL
                     GLPVILVAALGYTSVFGSATNTLLGPILIGGEVFGFANTPYFVIVCLVAYSISHAHTI
                     YGAQSR"
     gene            21197..21544
                     /gene="rplS"
                     /locus_tag="VBC94_000796"
     CDS             21197..21544
                     /gene="rplS"
                     /locus_tag="VBC94_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEO6979080.1"
                     /translation="MNPLIQSLTEGQLRSDIPEFRAGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            21655..21726
                     /locus_tag="VBC94_000797"
     tRNA            21655..21726
                     /locus_tag="VBC94_000797"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21688..21690,aa:Arg,seq:cct)
     gene            complement(21689..22303)
                     /locus_tag="VBC94_000798"
     CDS             complement(21689..22303)
                     /locus_tag="VBC94_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6979081.1"
                     /translation="MKFLETNEYLRLIKICKEKSQHQSYAALYLIAKTGMRFAECLGL
                     TVNDIDYTNKYLSINKTWDYHFNQRYLPTKNKSSIRNIPIDNDTLFFLHEFTKNKNDR
                     LFDKLSNNAVNKTIRKITGREVRVHSLRHTFASYLISISQVLDHENLNITLEVYAHQL
                     QEQKDRNDKLNQRNLGRIWGKIALNRYLHAMNMSPAGIEPAITP"
     gene            complement(22306..22572)
                     /locus_tag="VBC94_000799"
     CDS             complement(22306..22572)
                     /locus_tag="VBC94_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001881173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEO6979082.1"
                     /translation="MAYFRKRDNGWEYRISYKHIIGNILLCNITSTKYQQVINHYSQT
                     HVQDTVKRFNIHVKACVSMAVHEGYIEKNFCLFTKVKAKNKGRK"
     gene            22572..22976
                     /locus_tag="VBC94_000800"
     CDS             22572..22976
                     /locus_tag="VBC94_000800"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO6979083.1"
                     /translation="MIYSTLAKEQGVQGYLDGKGSLRDICKWYDISSRSVLQKWIKRY
                     TSGEDLKATSRGYSRMKQGRQATFEERVEIVNYTIAHGKDYQAAIEKFGVSYQQIYSW
                     VRKLEKNGSQGLVDRRVKGLESRPDLTEIEQL"
     gene            23138..23338
                     /locus_tag="VBC94_000801"
     CDS             23138..23338
                     /locus_tag="VBC94_000801"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6979084.1"
                     /translation="MPIQALCQLLKGSRSGYYKWLNRQKTDFETKNTKLMAKIKELHR
                     LYNGILGYRRMTTFINRQLGTT"
     gene            23363..23620
                     /locus_tag="VBC94_000802"
     CDS             23363..23620
                     /locus_tag="VBC94_000802"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6979085.1"
                     /translation="MNILGISSVIRRVSHACTKAGDRFYEENILNREFTATAHNQKWC
                     TDVTYLQYGLGAKAYLSAIKDLYNGSIIAYEISHNNEIHLL"
     gene            23746..23955
                     /locus_tag="VBC94_000803"
     CDS             23746..23955
                     /locus_tag="VBC94_000803"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6979086.1"
                     /translation="MSRIGKCIDNAPTESFFGFFKTESYHLKKYNSYDELVNDVARYI
                     EFYNTQRYQSKLNNLTPLEFRNQVA"
     gene            complement(24128..24289)
                     /locus_tag="VBC94_000804"
     CDS             complement(24128..24289)
                     /locus_tag="VBC94_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEO6979087.1"
                     /translation="MGGIGAHKFYAGKIGQVFLYIIFSITFIPGIIDLIEALMALGKQ
                     TDEYENIIV"
BASE COUNT         7843 a         3871 c         4843 g         7775 t
ORIGIN      
        1 tttcatctct ttttattaat tgtagatatc atgctagtgg ataaaatttg aaataaacgt
       61 agccaattgt ttttcagatg atttgtgaga aagggtagct ttaccatccc ctttttgatg
      121 tccgtagtta ccaaatcggc tatggtttcc cccgacaata gttcgaaaag tacttgaatt
      181 tgggaggcgt tttttagctt cctcatattt ttcccagttt aaaatatgat cgttacttgc
      241 tgtaatggag agtactctta actttttatg agataggtcg gatttacttg aagggtagga
      301 agctaataat atgagccctc taacaggggc tactttggca ttttggctag caacaactcc
      361 tcctaaagaa tgtccagcaa ggtaaacatt agttaagtgg ttctgattaa taattgtttt
      421 agctttataa ggtgataaaa ctggcaaatt taaaggtgtc ttcaaaatat aggtattata
      481 ccctttatca gctaaatctc tagcaagttg agaataagct tcttcttcca caagagcccc
      541 ttgatagaaa ataatatttg cttttacctt acccttgctt ctaaaaagaa ggtagttctt
      601 atggtttgta gcggttttag caattctcct agctaatggg gaggcttggt aagtattgaa
      661 gtaaatggtc atcccgacgg ccattaaaat agcaaggata cttgttgtta accaaagaaa
      721 aatagagtga cgttttatgt tcattggaag aaaggattaa agtttttttc gtggccaata
      781 gttgtattca tgccatggcc tgggtgaaca ctataatgac taggtaaggt gaatagttct
      841 tggcgaatac cagtaatgag gtcctcaaag ttactggtag gtaagtcagt acgaccgata
      901 gtttcacgaa atagagcatc gccagtaacg actaattcat ctgagtggaa aacaaatgag
      961 actcctcccc aagaatgacc aggtgttggt aaaactgtga attcaaaacc atttagttga
     1021 tagggttgct ttaatttaaa aaagttctct gccggtcttg ctataacatt aatgatatca
     1081 tcatgacggc ctaacccaga taaattatca tctggggaag atagccaagc ggcttctttt
     1141 tctgaaacat aaacaggtgg attatcaaaa gtatctctga ccaaatctaa actaaaaata
     1201 tgatcatagt gagtatgagt taagaggata gccactaaag gtttttcaaa ggatttaatt
     1261 ttagcaatga tttcttggcc attgctccct ggatcaatga ggataacagc ttgatcatta
     1321 acgaggtaat aagtattttc accagcaatg tggtttattg ttttatagat tttcataatg
     1381 atagtgtacc aaaaaatcaa gactttgtta aaaaaagagt ggcggaaaat aaatggcaac
     1441 tagtttcaga aaatatggga tctcacagca atttcttgac accgttttca taaaggtgta
     1501 tcataaaagt aatcaataag ccaagcctaa agctgaactt agtttaagaa cactgacttt
     1561 ttgagagcaa cctaattggt tgactaaaga atatgaggtg gaagatatgt caaaagttgc
     1621 tattgttact ggtgctggtc aaggaattgg atttgctatt gcgaaacgtc ttcatgcaga
     1681 cggctttaaa ataggcgttt tagactataa tgaagagact gctcaggctg cggttgataa
     1741 actatcacct gaggatgctg ttgcagttgt agcagatgtt tcaaaacgtg atcaagtctt
     1801 tgatgctttc caaaaagttg tagatacttt tggtgatttg aatgttgttg ttaacaatgc
     1861 aggtattgcc ccaacaaccc ctcttgacac tattactgaa gagcagtttg aaaaggcttt
     1921 tgcaatcaat gtcggaggaa ctatttgggg aagtcaagca gctcaaaaac attttagaga
     1981 attaggtcat ggcggaaaaa ttataaatgc aacttcacaa gcaggatgtg aagggaatcc
     2041 taatttgact gtttatggtg gtacaaaatt tgcagttcgt ggtattacac agactttggc
     2101 aaaggatttg gcatcagaag gaatcacagt caatgcctac gcaccaggta ttgttaaaac
     2161 acctatgatg tttgatattg tccatgaagt tggtaagaat gcaggcaaag atgatgaatg
     2221 gggtatggag cagtttgcaa aagatatcac tttgaaacgc ttgtctgaac cagaagatgt
     2281 tgctaatgca gtaggcttct tagcgggaga tgattcaaat tatatcactg gacaaacaat
     2341 agttgtcgat ggtggtatgg tattccatta aaaatgatta atgctaacac tgtgagtgaa
     2401 actcacagtg ttttttatat ttcttaaaaa attatcttat cttggttatt ggagtgatgg
     2461 catctttgag gtacagtcat gttttgtttt atagatatag cgtgctataa tagacttact
     2521 atgacccaga aaaaacttag aaaatatgca gtagttgact tggaggctac tggagcaggt
     2581 cccaatgctt ctattattca ggttgggatt gttattatcc aaggaaataa aattattgat
     2641 agttatgaaa cagatgttaa tcctcatgaa tctttggatg agcatattgt tcatttgaca
     2701 ggtattacgg ataagcagtt ggttaaagct ccggattttg gtcaagtggc gcatcacatt
     2761 tatcaattga ttgaagattg tatttttgtt gcccacaatg taaagttcga tgcaaattta
     2821 ttggctgagc aactattttt agaaggcttc gagttacgta caccaaggat agatacagta
     2881 gagttatcac aagtgtttta tccctgttta gaaaaatatt ctttaggggc attagcagaa
     2941 agcttaaata ttgaattaac agatgcgcac actgctattg cagatgcaag agctacagca
     3001 cagttattca ttaagttaaa agctaaaatt agtagtttac ctaaagaagt gttggagacc
     3061 attttaacat ttgctgataa tcttttgttc gaatcctatt tattgattga agaagcttat
     3121 caagaagctg atttcgttaa tcctaaagaa tattatttct ggcaaggatt ggtcttaaaa
     3181 aaagaaaaag ctgttggaaa gcctaagaaa ttatctagtg attttcaagt taacatggct
     3241 ttgttaggta tggatgctcg ccctaagcaa attgtttttg ctgacttggt taaagcgcat
     3301 tttaatgatc aaacaacaac gtttttagaa gctcaatcag gacttggtaa aacatatgga
     3361 tatctcttgc ctttattgga tcagtctcaa aaacaacaaa taatagtatc tgttccaaca
     3421 aagattttgc aagatcaaat aatggctaaa gaaataaagc acattcaaga actttttcat
     3481 attccctgtc acagtatcaa aggaccgaga aattacttga agttagatgc gttttataag
     3541 agcctccaag tgcaggatcg aaatcgattg ataaatcgtt ttaaaatgca actgctagtg
     3601 tggttgacgg aaacgactac aggtgatttg gatgaaatta aacaaaaaca acgattagaa
     3661 tcttactttg accagctaaa acacgatgga gaagtgactc aatcatcact tttttatgat
     3721 ctggattttt ggaaacgcag ttatgataaa gttgcacaaa gtcaacttgt tattattaat
     3781 catgcttatt ttcttgagcg tgttcaggat gacaaagatt ttgcaaaggg gaaggtttta
     3841 gtttttgatg aagctcagaa acttgttttg ggactggaaa atttttctcg tgggcaactt
     3901 gatattagtc accaattgca ggttattcaa aaaataattg atagtcctat tccgttatta
     3961 caaaagcgcc ttctggaaag tatttcttat gagttaagtc atgcagcaga acttttttat
     4021 cgtcataatt cttttgaatt ttcagagacc tggttgaaac gtctaaaaaa tagtataaac
     4081 gctttagaag tagtaggatt agatgattta caaacttttt tcacagctac ttatactaac
     4141 tattggtttg aaactgataa agtcaatgag aagcgtttaa ccattttacg tggggcgaga
     4201 gaagattttc ttaaattttc agagttcctc cctcccacaa aaaagaccta tatgatatca
     4261 gctactttac aaatcagtcc caaagtctac ttgtcagatc ttttaggagg attttcaagt
     4321 ataagcacag agaagatagc gcatgagaaa aatgccaatc agaaagtatg gattgatacc
     4381 tctatgccta atatacttga tttatcaccg gaacagtatg cttacgaaat agccaagcgt
     4441 ttacaagata taatgacgtt aaagcagcct accttagtct tattaacttc taaacagaca
     4501 atgtttatgg tatctgatta tttggataag tgggagataa aacatcttac tcaagataaa
     4561 aatggcttag cttataacgt taaaaaacgt tttgatcgtg gagaaagcaa tcttctttta
     4621 ggtacaggat ctttttggga aggtgttgac tttgtacatc gtgatcgtct tattgaagtt
     4681 atcacgcgat tgccttttga taatcccaaa gactatttta tccagaaact atcgcagagt
     4741 ttaacaaaag aggggaaaaa cttcttttat gactattctt taccaatgac tgtcttaaaa
     4801 ttaaaacagg ccttgggacg tactacaagg agagaagaac aaaagtcagc ggtaattatt
     4861 ttggatagca gattggttat taaatcttat ggacaaacca tcatgcattc tcttggtaga
     4921 gattttgaaa tttctaagga aaaaattaac aaggtcttga ctgaaatggc taaatttttg
     4981 atataatggt aaagatcatt gtctaaaaat aagatgttga cagtgcgact caagattaaa
     5041 taatagtagt acgttgataa aaggaggagt gatgacctat ttgtcagaac gtgtactcaa
     5101 tatggaagag agtgttactt tagctgcggg agctaaagca agagaactca gagtacaagg
     5161 aagagatatt cttagtttaa ctttagggga gcctgatttc gctactccta aaaacattca
     5221 gcaagcggct attgaggcta taacagatgg acgtgcaagt ttttacacac catcaagtgg
     5281 tttgccggag ttgaagtcag caattaatgc gtattttgaa cgtttttatg gctactccct
     5341 taaacctaat caggtggttg tgggaactgg tgctaagttt atcctttata ctttctttat
     5401 gacagtactg aatcccggtg atgaagtaat tattccgact ccttattggg tatcttatgc
     5461 tgatcagatt aagatggctg aaggtaagcc agtttttgtc acagcaaaag aagttaatca
     5521 cttcaaagta actgtggaac agttagaagc tgttaggact gataagacaa aagtcatact
     5581 tcttaatagt ccatcaaatc ctacaggtat gatttataaa gctgaggagt tggaggctat
     5641 tggaaattgg gcagtcgaac atgatatcct aattttagca gatgatattt acggtcgatt
     5701 ggtttacaat ggcaatattt ttacccctat ttcaagcctt tcagagtcca ttcgaaacca
     5761 aacgatagtc ataaatggag tttctaagac ttatgcgatg acaggttggc gtgttggttt
     5821 tgcagtgggt aatcatgata ttatagcggc aatgtctaaa gtagtcagtc aaacaacttc
     5881 taaccttacg gctgtctcac agtatgctac tattgaggct ttaaatggtt ctcaagagtc
     5941 atttgaaaaa atgaggctag catttgaaga acgccttaat atcatttatc cattgttgtg
     6001 tcaggtccct gggtttgagg ttgttaaacc acaaggtgct ttttatcttt tcccaaatgt
     6061 tacaaaagca atggaaatga aaggatatac ggatgttaca gcattcacgg atgccattct
     6121 tgaagaagtt ggacttgctt tagtaacggg tgctggtttt ggagcgccgg aaaatgttcg
     6181 tctgagttat gctacagatc ttggaaccct taaagaagct gttcgtagac tgcatgtctt
     6241 tatgggaagt aatgaaatta attaaataaa aatagagaga aacttatgtc aaaaaaactt
     6301 atttcaattg ttgatgttaa agattatgtt ggtcaagaag ttactattgg tgcttgggtt
     6361 gctaacaaat caggtaaagg taagattgcg tttgtacagt tgcgcgatgg ttctgccttt
     6421 ttccaaggtg tcgcttttaa accaaacttc attgaaaaat atggtgaaga atcaggtctt
     6481 gaaaaatttg acgttattaa acgtctcaac caagaaacat ctgtttatgt aacagggatt
     6541 gttaaagagg atgaacgttc aaaatttggc tatgagcttg atattactga ccttgaagtc
     6601 attggagagt cacatgaata tcctataact cctaaagagc atgggacaga cttcttgatg
     6661 gacaaccgtc atctttggtt gcgttctcgt aaacaaatgg ctgttatgca aattcgtaac
     6721 gctattattt attcaactta tgaattcttt gaccaaaatg gttttatcaa atttgatagc
     6781 ccaattttat cagaaaatgc agcagaagat tcaacagagt tattcgaaac agactatttt
     6841 gggaaaccag catttcttag ccaatctggt caattatacc ttgaagctgg agcaatggct
     6901 ctaggccgag ttttcgactt tggtccagtc tttcgtgctg aaaaatctaa aacacgtcgc
     6961 catttaacag aattttggat gatggatgcc gagtattctt tcttgtcaca tgaggaatca
     7021 cttgaccttc aagaagctta tgttaaagca ttgatccaag gcgttctaga tcgtgcgcct
     7081 caagcacttg atattttaga acgtgatgtt gaggctctca aacgttatat cgcagagcca
     7141 ttcaagcgtg tttcttatga tgatgctatt acacttcttc aagagcatga agcagatgaa
     7201 gatacagatt atgaacattt agagcatggt gatgactttg gttcaccaca tgaaacttgg
     7261 atttctaatt actttggagt gccgacattt gtagtgaatt acccagcaag ctttaaagca
     7321 ttttatatga aaccagttcc tggaaatcca gaacgcgttc tttgtgctga tcttttagct
     7381 ccagaaggtt atggagagat cattggcggc tcaatgcgtg aagacaacta cgatgctctt
     7441 gtggctaaaa tggatgaact aggtatggat aaatcagaat acgactttta ccttgacctt
     7501 cgtaaatacg gctctgttcc tcatggcggt tttggtatag gtattgaacg tatggtaact
     7561 tttgttgctg gtaccaaaca tattcgtgaa gctattccat tcccacgtat gttacatcgt
     7621 atcaaaccat aataaaaaag cgctagctag tgcgcttttt tattatggtc tttagaatcg
     7681 ttggttaatt aaattgggac tatgtttttt taacaatgtg attgtcgaaa agacaaggat
     7741 aagactgatt agtcgatcaa tataatctgt tccaacttga acaagtatca aactacttgt
     7801 catgtctaat ccaattccat gtaagaattg tgctataata cttgagccac tagaggtaat
     7861 tcctttaaaa aggataactg ttataactga agcaataata gttcctggta aagaaataat
     7921 cagtgatttc cacaataggc tagagggttt acaattcctt ttaatcaaga taccagtaat
     7981 gatagcaact acgatggcga ctggtgaata ataaagtgaa aaaatatcag tagtcatcca
     8041 attgatgagt gcactgagaa aagcagtgct ggcagcgtac catggtccca ggataactgc
     8101 tattagcaat gtacctatag tatctagata gataggtagg cgtaaaaata gagctaaatt
     8161 agccccaata aggttgatgg cgatagcgat tgagataaat gaaattaaac gagcaggtga
     8221 ttttgtcatt tgttaagtcc taacatttct aagtcttcaa tgatgactga attttgtgca
     8281 tgtaatagga cggtcataaa atcattccaa aataaattag tattgactga ggttaaaatc
     8341 ttactgttag catctttttt ataaaaatga tactgatcta caattgtttg tcccatggcg
     8401 attccgtgac aagcaacatc ggtatagcta tcaaaaccag tagcgatatt ttcattaaca
     8461 aagtaagcaa tagctaaagg atcatttatc acacatccaa taatatgctc atattgccag
     8521 tgaaaatcga agtagaactt tgtgattttt tggataaaag ttgatacgtc tggattaata
     8581 cgctccatat aagataaatg attgggggtt aatacaatat gacgtgtgat gtctagtcca
     8641 accatctcta tttttttgtc aaggttttca aatacatatt gtgcagcatg gggatcatac
     8701 caatagttgt attcagcaac tggagaacaa ttgccatgtg atttaaaatt tcctcccatg
     8761 ctaacaaatc gcttgcaatg tttgcctaat ttgggattag tttgtaaggc tcgagcgata
     8821 ttggtcagag gtccgagagc tataatagaa gtatcatttt tgtgttcaaa atagtttgct
     8881 aaaaaacaat ctgctgattc ttcttgaaag ataattggtt gcgccaatgt aaagttattc
     8941 tctccaaggc catccattcc atgagtatct tgagcagata caaattcacg ttgtaaaggc
     9001 aagttattac caacataaac tgaaatgtcg cgacgattta gtaattcaag cgttacaaag
     9061 gtattcttta gtcctagctc aacaggcgaa ttgcctgctg ttatagtaat agcaacaact
     9121 tctaacttag gatgctgaat agcatacatc agtgctaagg tatcatcaat accgggatcg
     9181 caatcgataa taattttttc tttgttcatt tgtctccttt cttttaatat tagtgaatgt
     9241 tctatgcttt tatgaaacta ttaacaatag tcatggcaat aaaaaagatc ttggcagtta
     9301 ccaagatcac acgaaaaaat gaatttagtt ttctaaattc aatcatattt tcagtcactt
     9361 agttttttat tctgggaagt tttcgaacca gtccgagaaa tcctcgatta tgcacgtgtt
     9421 ttattataat atgaaagcac taacttgtaa agattcaaac tgaccattta acagtgtcta
     9481 gattgattgt tttagcattt gaaattaatt ttgaaatcgg acatctgctg tgtgctttgt
     9541 taacgattag cttactatca gataatgaca gtttttcaat actagcaaca gcgttaactt
     9601 ggaaataaaa tcccttacct acttgctttt ccttcatgag ctggcaatta acatcaacac
     9661 gagattttag gtctttgaat cctttttgtt ctaaaatagc ttttatagtt gcatttaaac
     9721 atgttgacca tgctaaagca attaattctt caggattaaa tcctggtaaa tgatttaaag
     9781 ggtgattggt ctccacaatt tttccattgg agagataaac ttttccatca acgccttgag
     9841 tgtttgatga ggttacttca taaagcataa taaactcctt tgggtctagt ttaccaaatt
     9901 tgggagcatt ccgacaagat tttactattt agaatgagtt tcttttcctc tgaagtaaaa
     9961 tcctaattgc tagccatttg tggtataatg gcataaaaca taagagaaag aggtaatcgt
    10021 aatgtcagac gaacaaatca aacttgtgat tgtaacaggt atgagtgggg caggaaagac
    10081 agtggctatt cagtcatttg aagatttagg atattttaca atagataata tgccgcctac
    10141 tctagtgcct aagtttttag aattagcagc acaatcagga gatacaagca agattgctat
    10201 ggtagttgac atgcgtagtc gtcttttctt tagagaaatt aactctattt tggatagcct
    10261 tgaaattaat gataatatta actttaagat tctattttta gatgctactg acaccgagct
    10321 agtgtcacga tataaagaga cacgtcgtag ccaccctctt gctgccgacg gcagagtgtt
    10381 agatggtatt tctctagaga gagaactatt agcaccgctt aaaagtatga gtcagaacgt
    10441 tgtggacact agtgaattaa caccaagaca actacgtaag gtcatttctg aagaattctc
    10501 aaatcaagat agtcaaagtt cttttcgtat tgaagttatg agttttgggt ttaaatatgg
    10561 tatacctttg gatgcggatt tagtattcga tgtgcgtttt ttaccaaatc catactataa
    10621 accagaatta agagataaga caggtttaga tactgaggtt tatgactatg tcatgagttt
    10681 tgatgaatca gacgattttt atgaccatct tcttgcctta ataaagccaa tcttaccagg
    10741 ctatcaaaaa gaagggaaat ctgttttgac tgtggcaatc ggatgtacag gtgggcaaca
    10801 tcgtagcacc gcttttgctc atagattatc agaagattta aaagcagatt ggacagtgaa
    10861 tgaaagccat cgtgataaaa ataagcgtaa ggagacggtg aatcgttcat gagaaaacct
    10921 aaaataaccg ttattggcgg aggaacggga attccagtta ttttgaaaag tcttcgctta
    10981 gaagatgttg agattacagc ggttgtcacg gtggcagatg atggtggttc gtcaggtgag
    11041 ttgcgctcag ttatgcaact aacaccgcca ggagatttaa gaaatgtttt ggttgccttg
    11101 agtgatatgc cgaagtttta cgaacaaatt ttccaatatc gttttgcaga aggtgatggg
    11161 gattttgcag gtcatccttt aggaaatctt attattgctg gtgttgctga aatgcaaggt
    11221 tcaacttata acgctatgca gagtttaaca cagttctttc atacaacagg aaagatatat
    11281 ccatctagtg aacatccgct cactctgcat gctgttttta aagatggcca tgaagtggtt
    11341 ggagagagtc aaatagccga ttataaaggc atgattgacc atgtgtatgt cactaatact
    11401 tataatgaag agactcctac ggccagccgc aaagttgttg atgcaatttt agaaagtgat
    11461 atgattgtgt tgggtccagg gtcacttttc acctctattt tacctaacct agttattcca
    11521 gagattaaac aagctctttt agaaactcgg gcagaggtag cttatgtatg caatattatg
    11581 acacaacgtg gtgaaactga gcattttaca gacgcagacc acgttgaagt attgaaacgt
    11641 catttaggac aagatgctat cgatacagtt ttggttaata ttgaaaaagt gccagagtct
    11701 tatatggaaa acaatcattt tgatgagtat ttggtacagg ttgaacatga tttttcaggg
    11761 cttagaaaac atgccagacg tgtcatctcg tctaactttt taaaattaga aaaaggtgga
    11821 gccttccatc atggtgactt cgttgttgag gaactaatga atttagtagg aacacattta
    11881 tgagttttac agtaaaagtc aaagaagagt tgctaggtca taagagtgaa aataagatgg
    11941 aactgtcagc tattatcaaa atgtcaggta gtttaggatt agctaaccac ggcctcaacc
    12001 tttctattac cacagaaaat gctaaaattg ctcgtcatat ttatagcatg ttagaagaac
    12061 attatcattt gcagccagaa atcaaatacc atcaaaaaac gaacttacga aagaatcgtg
    12121 tgtatactgt ttttattgaa gaaaaagttg atgttattct tgctgatttg aagttggctg
    12181 atgctttttt tggtattgaa actggtattg aacactctat attggacaat gatgaaaatg
    12241 gaagagctta tctcagggga gcattcttat caacgggtac tgttagagaa cctgattctg
    12301 gaaaatatca actagaaatt ttttctgttt atctagacca tgcacaagac ttagctaatc
    12361 ttatgaaaaa atttatgtta gatgcaaagg ttattgaaca taaacatgga gcagtaactt
    12421 atttgcaaaa agcagaagat attatggatt tcttgatagt cattgatgcc atggaagcta
    12481 gagatgcttt tgaagagatt aaaatgatac gtgagacacg taatgatatt aaccgtgcta
    12541 acaacgttga aacagctaat attgctagaa caatcacagc tagtatgaag actatcaata
    12601 atattatcaa gattatggat actattggtt ttgatgcact gcctagtgat ttaagacaag
    12661 tagctcaggt gcgtgtggct catccagatt actctattca acaaatagca gacagtttag
    12721 aaacgcctct gagtaagagt ggcgttaacc atcgcttacg gaaaataaat aaaattgcag
    12781 atgaattata gaaggagata ttagcatggc ttgtacaaca atattggttg gtaaaaaggc
    12841 ttcttatgat ggttcgacta tgatcgctag aacggaagac tctgttaatg gcgatttcac
    12901 acccaaaaaa ttaaaggtaa tgacatctaa agatcaaccg cgtcattaca aatcagtttt
    12961 atcaaatttt gaagtagatt taccagataa cccacttcct tatacttcag taccggacgc
    13021 attgggaaaa gatggtatat ggggtgaagc cggtattaac agtaaaaatg tagcgatgag
    13081 tgctacagaa actattacaa cgaattcccg cgttttgggt gcagatcctt tggtttcaga
    13141 tggtataggg gaagaggata tactcacttt agtgcttccc tatattcagt cagcgcgaga
    13201 aggtgtggag cgtttaggtg ctattttgga aaaatatgga acctatgaat caaatggtat
    13261 tgctttttca gataccgaag aaatatggtg gttagaaaca attggtgggc atcattggat
    13321 tgctcgtcgc gtacctgatg atgtttatgt tactaatcct aaccaactag gaattgatca
    13381 ttttgaattt aataactgtg atgactacat gtgctctagt gatttgaaag agtttatcga
    13441 acaataccat ttagatttga cctattctaa tgagcatttc aatcctcgat atgcttttgg
    13501 tagccaacgt gataaagatc gtcattacaa cacaccaaga agttgggcaa tgcagcgttt
    13561 tttaaatcct gaaattgaac aggatccacg tagtttgttt attccctggt gtcaaaagcc
    13621 ttaccgaaaa attactgttg aggatattaa atatgtgttg agtgatcatt atcaagacag
    13681 tgtgtatgac ccatatggac cagaagggga tgcggtaagt aggagagctt ttcgttcagt
    13741 tggtatcaac cgaactagtc aaacgtctat tctacaattg cgaccaaata aatcacttga
    13801 aacgacaggt gttcaatggt tatcttatgg ctctatgcca tttgcaacca tggtgccgtt
    13861 gtttacacaa gttgagactg taccagacta tttttcgaat acaaccaagg atgcttcaac
    13921 agataatttt tattggacca atcgtttaat tgcagctcta gcagatccac acttttatca
    13981 acatgaagct gatattgaaa gctatatcga gagaacgatg gcccaaggac atgcacatat
    14041 taatggtatt gatagagaag ttgctgagaa taaagagatt gattttcaac agaaaaatca
    14101 agaaatgagt gactatattc aaaaagaaag ccaagaattg ttaaatcgta ttctatttga
    14161 tgcaagtaat ttaatgacaa atcgcttttc aatgggagat taaaaaactc ggaatatttt
    14221 ccgggttttt taagataaaa acgcttgcat aagtatataa aacttgcaaa aaaaaaatat
    14281 cgtgctatac tttactagta aaatattaac cggtaaataa cgggttaaat aaaaaggaga
    14341 aaaaatgaga aagaaatttc ttttattgat aagctttgta gctatgtttg cagcttggca
    14401 acttgttcaa gttaaacaag tttgggctga tagtaaactt aaagtggtaa caacttttta
    14461 cccagtttat gagtttacaa aaaatgtcgt tggtgataaa gctgatgtat ctatgttaat
    14521 taaagcaggt acagagccgc atgattttga accatcaact aaaaacatcg ctgccatcca
    14581 agattcaaat gcttttgttt acatggatga taacatggaa acttgggctc caaaagtagc
    14641 taagtcagtt aaatccaaaa aagtaacaac tattaaaggt actggcgata tgttacttac
    14701 taaaggcgtc gaagaagaag gtgaagaaca tgaaggacat ggtcatgaag ggcatcatca
    14761 tgaacttgac ccacacgtat ggttgtctcc agaacgtgcg atttctgttg tagaaaacat
    14821 ccgtaataaa tttgtcaaag cttatccaaa agatgcagct tcatttaata aaaatgcaga
    14881 tgcttacatt gcaaaattaa aagagcttga caaagaatac aaaaatggtt tgtcaaatgc
    14941 taaacaaaag agttttgtga ctcaacacgc agcgtttggt tacatggcgc ttgattacgg
    15001 tttaaatcaa gttccaattg ctggtcttac tccagatgca gaaccttcat caaaacgttt
    15061 aggcgaatta gctaaataca tcaagaaata taacatcaac tacatttatt ttgaagaaaa
    15121 tgcttcaaat aaagttgcta aaactttagc agatgaagtt ggcgtgaaaa cagctgtgct
    15181 tagtccactt gaaggacttt ctaaaaaaga aatggcagct ggcgaagatt acttctcagt
    15241 tatgagacgt aatttgaaag ttcttaaaaa gacaacagat gttgcaggta aagaagtagc
    15301 tcctgaagaa gataaaacta aaacagttga aacaggttac tttaaaacta aagatgttaa
    15361 agaccgtaaa ttgacagatt actctggtaa ttggcaatca gtatatcctc ttcttcaaga
    15421 tggaacactt gatccagttt gggattacaa agctaaatct aaaaaagata tgactgctgc
    15481 agagtacaaa aaatattata cagcaggtta caagactgac gtagaatcaa tcaagattga
    15541 tggtaaaaaa caccaaatga cctttgtacg taatggtaaa tcacaaacat ttacatacaa
    15601 atatgcaggt tacaaaatct taacttataa aaaaggtaat cgtggagtac gttatctctt
    15661 tgaagctaag gaaaaagatg ctggtcaatt caaatatatc caatttagtg accatggtat
    15721 taaaccgaat aaagctgaac acttccatat cttctggggt tcagaaagcc aagaaaaatt
    15781 atttgaggaa atggaaaact ggccaacata cttcccagct aaaatgtctg gacgtgaagt
    15841 tgcccaagac cttatgtctc attaatattt aaaagatgat atcggatacg atatcatctt
    15901 tttttcttcg tacgtgatag gctcaaacat agaaaaaagc acttctatta gaagtgcttt
    15961 taaaattaat gctatgctta ttgagcagcg tttgcgtctt tgagaccata ttttttgttg
    16021 aaacggtcca cacgtccatc tgcttgagtg aatttttgac gtcctgtata gaatgggtgt
    16081 gaatctgatg agatttccac acggataagt gggtaagttt caccttcgaa ttctactgtt
    16141 tctttagttg atttagttga accactaagg aatttgtaac ctgtagttgt atctaagaaa
    16201 acaactggac gataatctgg atggatgtct tttttcatct ttaaaaattt cctttctgcc
    16261 ataactcgtc tgagtcatag ttattaacca ctctattcta tcaaataaga atagccttga
    16321 caagtgtttt gataaaaaaa gtaaaattat aaagaaagta cttcctggat ttcttggtaa
    16381 atagcttgat tctcttccaa actataagaa ttagctccac tggctaaagg atggccgcct
    16441 ccattatgac gtttagctat ttcattaatg atgtgtgact tactacgtag gcgcacacga
    16501 taatgcccat cgctttgttc aacgaaaatt gcccaacttt ctacaatatc aatttttcca
    16561 ggtataccaa cgattgcagc agtctctgca tctgttacat caaagcgttt taagtcttct
    16621 tgtgttaaag taacgcatgc agcgccgttc ttgtcaattt caagttgctc aaaaataaag
    16681 ccttgtaatt ttgcaatttt gaaaggaaag gaatccattt gacgtgccat tgcagaaaaa
    16741 tcaaagtcaa attcacgaag tttgctagca atcttcagtg ttttacttgt ggtagcagga
    16801 tataaaaagc gtcctgtatc tcctacaata ccattatata gaactcttgc tgcacttgtt
    16861 gataacaaca aatcacaaga aagagcaaag tctgtcacaa tttcacttgc actagaggcg
    16921 ttagtatcta cataagagag gtctccatag acttcatcgt ttggatgatg gtcaatttta
    16981 attaaaaagt cacctttttt ataacgttca tcatcaatgc gaggggtatt tgctgtatct
    17041 gtaacaataa caagagcgcc ttgatagtct tggtcggtca cttgatccat tttagcaatc
    17101 caagcgagtg tgggttcgtc aaaaccagta gcaagtactt ttttctttgg gaaattatgc
    17161 ctgatgatat cccttaagcc gatttggcta ccaagagcat cagggtctgg tctcatatgt
    17221 cgatggataa tgatggtgtc gtactctttg attttatcaa gaatttgttg aaaaataatc
    17281 attttttacc tacctaactg ttttgtcttt tagtgaagaa caaggctata ttgttgacta
    17341 tattatagct taaatctttt attgaggaaa gaaattaaga tagcggccgc aatgtttgtt
    17401 tatttttata tgatggtatg ttattttttt ataaaattcg gatactgcta actaacaatc
    17461 ttttttatga taatattttg taaatatatt gctttttagc taacttttgt taatatagta
    17521 aaaatgcttt ttaataggag ataaattatg acacaagcag tattaaaaga attggctaag
    17581 gcggagttgc attgtcatct agatggctca ctttctttac cagctattag aaaattggct
    17641 aatatggctg atataatact gccaaatagt gataaagaat taagaaaata tgttatagct
    17701 ccagctcaaa cagaaagctt ggtagattac cttaaaacgt ttgagtttat cagaccgctt
    17761 cttcaaacga aggaagcatt acgttttgct gcttatgatg ttgctagaca ggcagcttta
    17821 gaaaatgtta tttatattga aattcgtttt gcgccagaac tctctatgga taaaggttta
    17881 accgcaagtg atacagtctt tgcagtctta gaaggtttag cagatgctca gaaagaattt
    17941 aatatcgttg cacgcgccct tgtctgcggt atgcgccaat cgtctcataa aacgaccaag
    18001 gaaattatta aacacattgt tgatttggct cccaaaggcc tggtaggttt tgattttgca
    18061 ggtgatgaat tttcctatcc aacagatagt ttagtagatc ttattcagga agtaaaacgc
    18121 tcaggttacc cgatgacttt acatgcaggt gagtgtggtt gtgctaagca tattgcggat
    18181 agtttaaatt tagggattaa gcgtatggga catgtgacag ctttaacagg acagagggct
    18241 ttaataaagc gatttgtcga agaagatgct gtagcagaga tgtgtttaac tagcaattta
    18301 caaacgaaag cagcctcaag tattcaaagt tttccttatc aagaacttta tgatgctgga
    18361 ggtaagatta caattaatac agataatcgt actgtttccg atacaaattt gacgaaagaa
    18421 tatagtcttt tcgtaactta ttttggaacg aaaatagaag actttcttgt ttttaatcaa
    18481 aacgctgtga aggcctcctt tacaagtgat tcagagaaag atactttact acataagcta
    18541 caagaaaact atgacagcta tctaaaataa ccttcacgat tctttaaaaa tatgatagaa
    18601 tagtggtaat ttacaagtgg aggaagatat ggctttagca aagattgttt atgctagcat
    18661 gaccggaaat actgaagaaa ttgctgacat tgttgctgat aaattacgtg acttgggtct
    18721 agatgttgaa gtagaagagt gtacgatggt tgatgctgca gattttgaag atgctgacat
    18781 tgcaattgtt gccacttata catatggtga tggagattta ccagatgaaa tcgtggactt
    18841 ttatgaagat ttagcagaag ttgatttatc tggtaaagtt tacggcgtag tgggttcagg
    18901 tgatactttc tatgattact tctgcaaatc tgttgatgag ttcgaggctc aattcgcatt
    18961 aacaggagca caaaaaggtg ctgattgtgt taaagttgat ttagcggcag aagacgaaga
    19021 cattgaaaac ttagaagctt ttgctgaaga aattgcttct aaacttaact aagctaccaa
    19081 aaagacgaac acgagtcgtc tttttaactt ttttaccatc ttttgtaact agaatatgag
    19141 atatagatat ggaattagaa gagattcgtc aagaaataga tgaaatagat cagcaattgg
    19201 taagcctttt agaaactaga atgggtctaa ttttagaggt catagcattt aaaaagaaac
    19261 accgacttcc agttttagat aataatcgtg aaaacgaagt gcttaacaat gtattgaaga
    19321 aggtccaaaa tcatcaattt gacgatgtga tacgagcaac tttcaaagac attatgacag
    19381 aatctcgtgt ttatcaaaag gagaatattg tagacggtga ttaaacgatt tgataattat
    19441 gctcagtacg tacttgtttt agggtttagc ttactaatcg gtggtgtggt tggggctatt
    19501 acagcagtct ttggacgtgt tctattattc ctgacagcct ttagaagtga ttatatagct
    19561 tacctactcc cttttcttag cattgttggt ttatttatag tttttgtcta tcaaaaattt
    19621 gggggtaaga gtgttaaagg gatgggattg gtatttgaag taggtcatgg taatgaggag
    19681 actattccga aacgtcttgt tcctctcgtg atattaacaa cttggttaac ccacttattt
    19741 ggtggtagtg caggtcgtga gggggttgct gtacagattg gggctaccgt ttcacactac
    19801 tttcaaaaat attgccgttt acaaaacgct tctcaacttt tcctagtaat gggaatggca
    19861 gcaggttttg ctggtttatt tcagactcct ttagctgcta ctttttttgc gatcgaagtt
    19921 ttggtagtag gtcgtttaat ggtttcgtat atattaccaa gtttaattgc tgctctgaca
    19981 gcaaatttcg tctcacattc tttaggttta gaaaaatttt ctcatagtat agcaacatca
    20041 atggcgttaa caccagatat tattctaaaa ttattggttt taggactatg ttttggtctg
    20101 tgtggcaatt tatttgctta tttattggct aaagctaaat taattgcttc tagtaggtta
    20161 ctaaacccat ataaacggat tttcacactt ggattgttag ctactttttt attgtttatc
    20221 ttccattttg gacgatattc aggactggga acaaatctaa ttgaggctag cttcactaat
    20281 aagaatcttt atgattatga ttggttgtta aaattgtgtt tgacagttat tactttggct
    20341 gcaggttatc agggaggaga agtaactccc ttatttgcaa taggagcaag tttaggagtg
    20401 attattgcgc ccattttagg tttgccagtt attttagttg ctgctttagg ctatactagc
    20461 gtttttggga gtgcaacgaa tactctgtta ggtcctatct taataggtgg cgaagtcttt
    20521 ggctttgcca acactcctta ttttgttatt gtatgtcttg ttgcttactc catatcacat
    20581 gctcatacga tttatggtgc tcaatctaga tagttagtga tgagttttaa aagagtttat
    20641 cagtttatag aacggaatat tccgttctct tttttattct cttaaagttt ataaaaatga
    20701 agtttgtaaa ttttatgaat gcaaaaatta tgtggctaat ataattttct tcaaagaaat
    20761 tgattttatg attgtcatta aaatactgta aaatccaatt gaatgtgtaa gaacttaggt
    20821 taggataatg tctacaagca tttaagtcgc gatggtgaaa tgcattagaa gataaaaagt
    20881 tgatgtggat ttcaacttta tgggaaagag tcaggtgaca cgaaagctaa tatagaaaac
    20941 gtagtttaaa aaatcatata atattaaatg aattaaactc tcaaaaatca gaaaaaagct
    21001 aacctagaaa cagtaaatca aatttcaata atatacaaat aactattcta aaggatgaca
    21061 ccttgttctt ctagataaaa tagctttaca agcaggttaa aatttggtag aattgtgaga
    21121 gtgtgtaatg gacgcacaaa aaacggctaa tccgctgaga caagtactta agattagtaa
    21181 gagaaggaga ataaaaatga atccattaat tcaaagtttg acagaaggtc aacttcgttc
    21241 tgatatccct gagttccgtg ctggtgatac tgtacgtgtt cacgctaaag ttgttgaagg
    21301 tactcgcgaa cgtattcaga tctttgaagg tgttgttatc tcacgtaaag gtcaaggaat
    21361 ctcagaaatg tacacagtac gtaaaatttc tggtggtatc ggtgtagagc gtacattccc
    21421 aattcacact cctcgtgttg ataaaatcga agttgttcgt tatggtaaag tacgtcgtgc
    21481 taaactttac tacttacgcg cattgcaagg taaagctgca cgtattaaag aaatccgtcg
    21541 ttaattttga tgatcagatt ttaaaaatgc ttggttgttt gaggatagta actatgtttt
    21601 aaaactggac aaccaagacg taaaaaatct gcctgtgggc agttttttta ctaggtcccc
    21661 ttagttcaat ggatataaca actccctcct aaggagtaat tgctggttcg attccggcag
    21721 gggacatatt cattgcatgt aaatagcggt ttagagctat tttgccccaa atccgcccca
    21781 aatttctctg attaagttta tcgttcctat ctttttgttc ttgtaattga tgtgcgtaaa
    21841 cttctaaagt gatatttaaa ttctcgtgat ctaaaacttg agagatggaa attagatagc
    21901 ttgcaaatgt atgcctgaga gagtgcactc gtacctcgcg accagttatt tttcggatag
    21961 ttttattgac tgcattattt gaaagtttgt cgaataatct gtcgttttta ttttttgtaa
    22021 attcatgcaa aaaaaataat gtatcattgt caattggtat atttctgata ctacttttgt
    22081 tttttgttgg caggtatctt tggttgaaat gataatccca agttttatta attgataaat
    22141 atttgttagt gtaatcaata tcattaactg ttaaacctaa acattcagcg aagcgcatgc
    22201 cagttttagc gatgaggtat aacgctgcat acgattgatg ttgtgatttt tctttacaaa
    22261 tttttatcaa gcgtaagtat tcattggttt caagaaattt tatctttatt tacgcccctt
    22321 attttttgct ttaaccttag tgaataaaca aaaatttttt tctatatatc cctcgtgaac
    22381 agccatggat acgcaggctt ttacatgtat gttaaaacgc tttactgtat cttgcacatg
    22441 cgtttgacta taatgattta tgacttgttg atatttagtg gaagtaatat tgcaaagtaa
    22501 tatatttcct attatatgtt tatacgatat tcgatattcc cacccgttgt cgcgtttacg
    22561 gaaatacgcc attgatatac tccacattag ctaaagaaca gggtgttcaa ggctaccttg
    22621 atggaaaagg ctctcttaga gatatttgta aatggtatga tatctcaagt cgctctgttc
    22681 tccaaaagtg gataaaacgg tatactagtg gtgaagactt gaaagccact agtagaggat
    22741 atagccgtat gaaacaagga aggcaagcca catttgaaga acgtgtagag attgttaact
    22801 acaccattgc ccatgggaaa gactatcaag cagctattga gaagtttggt gtttcctacc
    22861 aacaaattta ttcttgggtg cgtaagcttg agaagaatgg ctcacaaggt ttggttgata
    22921 gacgtgtgaa agggttggag agtaggcctg atttaaccga gattgagcaa ctttaactca
    22981 agattaaaca attggaggaa cgtaatcgtc tcttagaaat cgaggttagt ttactaaaaa
    23041 agttagaaga catcaaacga ggaaacagac ggtaagacta ggtaagcatt tagcggagtt
    23101 ccaagtaatc aagaattatt acgatgagga atctaatgtg cctattcagg ccttatgcca
    23161 actcttgaag gggtctcgtt caggctatta caagtggctc aatcgtcaaa aaacagattt
    23221 tgagacaaaa aatacaaagc taatggctaa aatcaaggaa cttcatagac tctacaatgg
    23281 tatcttaggt tatcgccgta tgacaacatt tattaatcgt caacttggga caacttaaaa
    23341 caagaaacgg attcgttgat tgatgaacat tctggggatt agttcagtca ttcgtcgtgt
    23401 tagccatgct tgtacaaaag ctggtgacag attttacgaa gaaaatattc ttaatcgtga
    23461 atttacagcc acagctcata accagaaatg gtgcacagat gtcacctatc ttcaatacgg
    23521 tctgggagct aaagcttatc tcagtgcgat taaagacctg tataacggtt ctattatcgc
    23581 ttatgagatt agtcacaaca atgaaatcca cttgttatga agaccattaa aaaggggcta
    23641 gagctcaatc caggagccac acctatcatc catagcgatt gaggtagtca atatacttcc
    23701 aaagaatacc gttatatcat acaacaagct ggtctgacct tatccatgtc ccggattggc
    23761 aaatgtattg ataatgcacc aactgaaagt ttctttgggt ttttcaagac tgagtcttac
    23821 caccttaaga aatacaactc ttatgatgag ttggtcaatg atgtggcacg ttatatcgaa
    23881 ttctacaaca cacaacgtta tcaatcaaaa ttaaacaatc tgactcctct agaattcagg
    23941 aatcaggttg cataacttat cttttattat ttgactgtct acttgacagg gagccgttca
    24001 gattgcttaa cctttctaaa tttgctaaaa tagctacaag aaaacgagcc atttaatgct
    24061 tatttcttat actgtcttgc ctcacgctct cctcgaccaa aaattgagcg tgaggctttt
    24121 tgtttcatta aacgatgata ttttcatatt catcagtttg ttttccgaga gccatcaaag
    24181 cttcgataag gtcgataatt ccaggaataa aggtaatact aaaaataata tataaaaaaa
    24241 cctggcctat ttttcctgcg taaaatttat gcgctccaat gccgcccaaa agaacgttaa
    24301 taaaacataa actactatgt tagcataaga ct
//
LOCUS       DATROQ010000022        24014 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.022, whole genome shotgun sequence.
ACCESSION   DATROQ010000022 DATROQ010000000
VERSION     DATROQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24014
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.022"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            155..907
                     /locus_tag="VBC94_000805"
     CDS             155..907
                     /locus_tag="VBC94_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6979088.1"
                     /translation="MFNKIGFRTWKSGKLWLYMGVLGSTIILGSSPVSAMDSVGNQSQ
                     GNVLERRQRDAENKSQGNVLERRQRDAENKSQGNVLERRQRDVENKSQGNVLERRQRD
                     AENKSQGNVLERRQRDVENKSQGNVLERRQRDAENKSQGNVLERRQRDAENKSQVGQL
                     IGKNPLFSKPTVSRENNHSSQGDSNKQSFSKKVSQVTNVANRPMLTNNSRTISVINKL
                     PKTGGDQNVIFKLVGFGLILLTSRCGLRRNEN"
     gene            1115..2191
                     /locus_tag="VBC94_000806"
     CDS             1115..2191
                     /locus_tag="VBC94_000806"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEO6979089.1"
                     /translation="MGYTVAIVGATGAVGTQMIRQLEQSNLPIEQVKLLSSSRSAGKI
                     LHFKDEAIRVEETTKESFYDVDIALFSAGGSISAKFAPYAVKSGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHAMIGHNGIIACPNCSTIQMMIALEPIRQKWGIERVIVSTYQAVSGS
                     GARAVEETKEQLRQVLNDNLSPDQLIATVLPCSSDQKHYPIAFNALPQIDIFTDNDYT
                     YEEMKMTLETKKIMEDATIKVSATCVRIPVLSGHSESIYIETKELASISEIKKAIANF
                     PGAVLQDLPSQQIYPQAINAVGHRETFVGRIRKDLDQENGVHMWVVSDNLLKGAAWNS
                     VQIAETLHKNGLVKPAKELKFELI"
     gene            2361..2699
                     /locus_tag="VBC94_000807"
     CDS             2361..2699
                     /locus_tag="VBC94_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979090.1"
                     /translation="MQIIKRDGQIVEFDPEKIYQAILKAAQTVYVLDESLRQDLAQVT
                     KKVILDLEDAQVERPTINMIQSLVENRLLDAGYIHIAEHYISYRLQRDLERNGYDDQI
                     TVHLHFEKIK"
     gene            2830..4371
                     /locus_tag="VBC94_000808"
     CDS             2830..4371
                     /locus_tag="VBC94_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6979091.1"
                     /translation="MSILEVKNLSHGFGDRAIFENVSFRLLKGEHIGLIGANGEGKST
                     FMSIVTGHLQPDEGKIEWSKYVTAGYLDQHTVLEEGQTVRDVLRTAFDELFKTEARIN
                     DIYISMADDGADVDSLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDSDVT
                     ELSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQDLVRYSGDYTNFESVYAMKKAQLEAAYERQQKEIADLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPSFEFKESRTPGRFIFQAKDLQIGY
                     DRALTKPLNLTFERNQKIAIVGANGIGKTTLLKSLLGIIPPISGNVERGDFIDLGYFE
                     QEVPGGNRQTPLEAVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQSKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKAYKGSILMVCHEPDFYEGWMDDVWD
                     FNQLS"
     gene            4411..5643
                     /locus_tag="VBC94_000809"
     CDS             4411..5643
                     /locus_tag="VBC94_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEO6979092.1"
                     /translation="MQNDMSVVLRAGKLLIESGAEVYRVEDTMKHFAKALQIENFEAY
                     VVSSSIIASGINRYGKQEAKVCNTDGVTANLGRLEAVNNLSRQIAKQDLVSPEEIVKQ
                     LDLIEHQKDYSLLVTLISYFCGAGSFSLALGSSLLDSFSAAVTGLILGYFLNLMESRI
                     HTGFLLTILGSSVVALSANLLYFSGLGEHRSIIILGALMVMVPGAAFVNSVREFSQNN
                     FSTGLALIMSALLICISISAGVAITIEIIPFAEQMTGSFSGVPNTILEIIIRTLMAGL
                     GTIAFSILYHVPKRYFLDLGILGAISWMLYLILWQQFHMDAIAVFFPGLFITYFSRLL
                     AAKRKCPATVFLATSMFPLIPGLSFYRAVYFLLTGADALAMEYFRSCFVTAFTIAIAI
                     SIVQQIPLSFFIRRKMIK"
     gene            5856..6557
                     /gene="pyrF"
                     /locus_tag="VBC94_000810"
     CDS             5856..6557
                     /gene="pyrF"
                     /locus_tag="VBC94_000810"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEO6979093.1"
                     /translation="MLEKCPIIALDFSDLASVTTFLEHFPKEELLFVKIGMELYYSEG
                     PSIIRYIKSLGHRIFLDLKLHDIPNTVRLSMSVLAKLGIDMTNVHAAGGVEMMKAARE
                     GLGEGPILLAVTQLTSTSQEQMQVDQHINLSVVDSVCHYAQKAQEAGLDGVVASAQEV
                     KQIKKQTNEHFICLTPGIRPPQTNQLDDQKRTMTPEQARIVGADYIVVGRPITKAENP
                     YQAYLDIKEEWNRIK"
     gene            6570..7199
                     /locus_tag="VBC94_000811"
     CDS             6570..7199
                     /locus_tag="VBC94_000811"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEO6979094.1"
                     /translation="MDLARQIAMELLDIQAVYLRPQQPFTWASGVKSPIYTDNRVTLS
                     YPETRTLIENGFVKQIQKHFPNVDIIAGTATAGIPHGAIIADKMNLPFAYIRSKAKDH
                     GVGNQIEGRVYSGQKMVIIEDLISTGGSVLEAVTAAQSQGIEVLGVVAIFTYQLAKAE
                     QAFREADIPLVTLTDYNQLIKVAKVNGYITADQLVLLKKFKEDQMNWQS"
     gene            7211..8503
                     /locus_tag="VBC94_000812"
     CDS             7211..8503
                     /locus_tag="VBC94_000812"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEO6979095.1"
                     /translation="MYIIKNGLIIDPQSGFNQVSDMLIDQGKIKQISKEIDIKGIPII
                     DASNKIVAPGLVDIHVHFREPGQTHKENIHTGALSAAAGGFTTVLMMANTNPTISSSE
                     IVKQVKESAAKEAIKIETVATITKSLNGKDLVNFEELLEAGVAGFSDDGIPLTDTKVL
                     QEAMNLARKHDVVLSLHEEDPSLNGVLGINEHIAQKIYHVCGASGLAEYSMIARDAMI
                     AYQTQAKVHIQHLSSSESVEVVDFAQKLGANLTAEVTPQHFSKTEDLLLTKGANAKLN
                     PPLRLEKDRQALIDGLKSGVISIIASDHAPHHIMEKAADNISQAPSGMTGLETSLALG
                     ITYLVSTKELSMIDFLAKMTCNPAQLYGFDAGYLREGGPADIVIFDQAEERIIKAEFA
                     SKSSNSPFIGDKLKGVVHYTICNGEIVYQKDSHAATLV"
     gene            8668..9591
                     /locus_tag="VBC94_000813"
     CDS             8668..9591
                     /locus_tag="VBC94_000813"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEO6979096.1"
                     /translation="MNHQTQTLSLEHFVSLEELSNQEVMSLIKRSIEVKENPSNIGFD
                     KDYYVSNLFFENSTRTHKSFEMAELKLGLKTIEFNADTSSVNKGETLYDTILTMSALG
                     LDVCVIRHPDIDYYKELIASPNIHSAIVNGGDGSGQHPSQSLLDLVTIYEEFGYFKGL
                     KIAIVGDLTHSRVAKSNMQVLKRLGAEIFFSGPKEWYSSQFDEYGQCLPIDQLVDQID
                     VLMLLRVQHERHDGKGVFSKESYHQQFGLTKERYKHLRDTAIIMHPAPVNRDVEIASD
                     LVEADKARIVKQMSNGVYARIAILEAVLNSR"
     gene            9605..10681
                     /gene="carA"
                     /locus_tag="VBC94_000814"
     CDS             9605..10681
                     /gene="carA"
                     /locus_tag="VBC94_000814"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEO6979097.1"
                     /translation="MKRLLLLEDGSVFEGEAFGADVGTSGEIVFSTGMTGYQESITDQ
                     SYNGQIITFTYPLIGNYGINRDDYESIRPTCKGVIVYEWAEYPSNWRQQMTFDEFLKL
                     KGIPGISGIDTRALTKIIRKHGTMKACLINEGNSIHEALENLQKSVLLNNQIEQVSTK
                     LAYASPGVGKNIVLVDFGLKHSILRELSQRQCHITVVPHTTTAQEILNLNPDGVLLSN
                     GPGNPEQLPNALQMIQEIQGKIPIFGICMGHQLFAKANGAKTYKMTFGHRGFNHAVRH
                     LQTGQVDFTSQNHGYAVSREDFPEALFITHEEINDKTVEGVRHKYYPAFSVQFHPDAA
                     PGPHDTSYLFDEFINMIDDFQQKS"
     gene            10712..13894
                     /gene="carB"
                     /locus_tag="VBC94_000815"
     CDS             10712..13894
                     /gene="carB"
                     /locus_tag="VBC94_000815"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEO6979098.1"
                     /translation="MPKRTDIRKIMVIGSGPIVIGQAAEFDYSGTQACLSLKEEGYQV
                     VLVNSNPATIMTDKDIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMAL
                     SKNGILEELNVELLGTKLSAIDKAEDRDLFKQLMEELNQPIPESEIVNSVEEAIQFAE
                     QIGYPLIVRPAFTLGGTGGGMCDNQEQLVDITTKGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDAADNALVVCNMENFDPVGIHTGDSIVFAPAQTLSDVENQLLRDASLDIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPITKTTYAMFEPALDYVVAKMPRFPFDKFESGDRKLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVDHIKIADLDNVSDDVLLEKIRKAEDDRLFYLAEALRRHYSIEKLAS
                     LTSIDSFFLDKLRVIVELEDLLSKNRLDINILKKVKNKGFSDKAIASLWQINEDQVRN
                     MRKEAGILPVYKMVDTCAAEFDSATPYFYSTYAVENESLISDKASILVLGSGPIRIGQ
                     GVEFDYSTVHSVKAIRESGFEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIDL
                     EKPEGVILQFGGQTAINLAKDLNKAGVKILGTQLEDLDRAENRKQFEATLQALNIPQP
                     PGFTATTEEEAVNAAQKIGYPVLVRPSYVLGGRAMKIVENEEDLRHYMTTAVKASPDH
                     PVLIDAYLIGKECEVDAISDGQNILIPGIMEHIERAGVHSGDSMAVYPPQTLSETIIE
                     TIVDYTKRLAIGLNCIGMMNIQFVIKDQKVYVIEVNPRAGRTLPFLSKVTHIPMAQVA
                     TKVILGDKLCNFTYGYDLYPASDMVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     INLQKALYKAFEAAYLHMPDYGNIVFTVDDTDKEEALELAKVYQSIGYRIYATQGTAI
                     YFDANGLETVLVGKLGENDRNHIPDLIKNGKIQAVINTVGQNNIDNHDALIIRRSAIE
                     QGVPLFTSLDTAHAMFKVLESKAFTLKVLD"
     gene            14020..14343
                     /locus_tag="VBC94_000816"
     CDS             14020..14343
                     /locus_tag="VBC94_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979099.1"
                     /translation="MLKTRTHINELETKIALDLVHRYLGIQQLVFSKWRHSLETGSWS
                     SIITINGANIINYIVDDISDSDSISGIICHPKSFHLRPREKKEPIQSIDELDIIWSLN
                     REENE"
     gene            14340..14810
                     /locus_tag="VBC94_000817"
     CDS             14340..14810
                     /locus_tag="VBC94_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HEO6979100.1"
                     /translation="MIPDSSFKVLSDMAYNIEKSKAESNGHLKIIEGSQIIDFHQKRS
                     FMVLEVNDNIDNGMQAMACQSLDESNHIVIAYSGTNAFNKKDILTDIDSVVLGKNNKL
                     LTCPSSITLREDGISLPCKNPSQITTSKLFAQKIKMNYPDATLWLTGHGKCSVR"
     gene            14788..17262
                     /locus_tag="VBC94_000818"
     CDS             14788..17262
                     /locus_tag="VBC94_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage infection protein"
                     /protein_id="HEO6979101.1"
                     /translation="MVNVQSGNISNYQKNLLDSATNFQNIFPALVNQYSSSITANESL
                     KKSLEASDNMFNDLMTTQTNTGKDLSSLIEQRHQDSISYEAFSASLLEMNNELLEKQL
                     SDIITQAQKDQETLSSQLNSIMGDDNDNDHNHKENSSAYLNVARQKIQELSEALKSQD
                     NIAKDQSEQLDKIVREGLASYFAKNNKDNITLLELLKSHSTNEKTLKDFKVKVADFTN
                     SLISSIPSLNLSELHLTQEEKAIQFTSSDSEIIKKVSNERTLYLNTNLLNRLFEARKN
                     RDEAKNKVNQLKLPSSSTRTGEQIVSVESNNPDYRVDTWTVNGKQTRTLDPSQNNNII
                     INSSYQGELSNSSITKPSYTITIGNQKQIVQNDDGEIQRSYLEAEATYQRTLQEVIDA
                     YNTTHELVAKYYIISDGEEPQNLFDQFLNQSVNDTMVDLVKNGITKYLMDENTADAQQ
                     KVKDVMEEVENSQDELADQMAKVTETNVRLTDTIKKQLETLQTINMKVQNITQDQSKV
                     NDSQKTTDQQLSDLKNQLDGLMTSATGVKDMSKSNSQEADQVNQIFTSFNKDVQDAKN
                     SGNKLSTDATDLMANFQKELANNGDFVASFSKVFNTAYKNGVPNDILLNFLSRPVAES
                     ASAVRATENTYRPFTWILLLEVVSLFIAYIFATQNLIKKLTDRYNVNRWLQTDFLNII
                     VISGLSLVIGLALGVISSRSLHVMPEYVPSWFLVMTLFSFLLIHSQYFFIKNFKAVGM
                     GLALFMIISFVYLSNAVGTVATVSGFPKLLKAINPLSILENQLSSYFDNVTTGFIFLI
                     LVLLVDVAFIIMNIFITLNFEAKVKE"
     gene            17271..17744
                     /gene="essA"
                     /locus_tag="VBC94_000819"
     CDS             17271..17744
                     /gene="essA"
                     /locus_tag="VBC94_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HEO6979102.1"
                     /translation="MKLKRFLACLLILSCMSQTSVFSDGLKNNDIHFDTKRLESDSQK
                     NSGFQTELTKDLFKKEDLKKLDKNRQSKVSLQKKQSKQLFQYSNKGQSDRKKRIKQLF
                     SEESNQFKFVTRTEEATVSADIGHMIMYLVLISLVLILASIMTFKIKDKKDEYTY"
     gene            17728..17970
                     /locus_tag="VBC94_000820"
     CDS             17728..17970
                     /locus_tag="VBC94_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HEO6979103.1"
                     /translation="MNTHINITLDMGETSKDLRIPLKISVKQLVMELDSIFSRTERRT
                     KYQLRVINKGILLGENDILAHHPVTTGDRIRIEEFY"
     gene            17970..19244
                     /gene="essB"
                     /locus_tag="VBC94_000821"
     CDS             17970..19244
                     /gene="essB"
                     /locus_tag="VBC94_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HEO6979104.1"
                     /translation="METDSFVFEEQDFQFEKGPDFWQLSLKRSKVGTQNLQQLQLLEI
                     HHSLLMPMTTAINADTIQFQFQTEAHALSFESFKKETLSEKLRLALNVLDLDKALSLP
                     VNFILHPSNLFLTKNATAKIAYRSLPGIMTPEKFGPEEFLYQFKCFVFALLTQHDYIE
                     LYNGAISVIEVSDFLKSIYHAETIQAVRDIITTDYEQQVEVETHTLAKVSKAKYKLYK
                     YISVWLGALSTILLIPLVYLVFIHNPFKEKMLAADTSFIKVDYNQVINRLEHVKVSKL
                     PYTQKYELAYSYINGMSFSEEQREVILNNVTLKTDELYLDYWINIGRGLDDDAIDAAK
                     RLDDSDLVIYAIVQKMDQVRKDNSLSGKDREQKLSELQTDYDKYWKDRKTALTDEESK
                     SKNSNNHSTNSNKESSESSSTTASTSSKTKSR"
     gene            19251..23680
                     /gene="essC"
                     /locus_tag="VBC94_000822"
                     /pseudo
     CDS             19251..23680
                     /gene="essC"
                     /locus_tag="VBC94_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
     gene            23685..>24014
                     /locus_tag="VBC94_000823"
     CDS             23685..>24014
                     /locus_tag="VBC94_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979105.1"
                     /translation="MKKQRLLLLFGGLLIMIMMTACKDSKIPENRTKKEYQAEQNFKS
                     YFKYISDKNNYLDNIKVYYFSISISKDVQDKVSETTTCSYRLEKQKNQEFIGNFEHEV
                     SESSQYST"
BASE COUNT         8176 a         3934 c         4553 g         7351 t
ORIGIN      
        1 actatttaat tttaagcata caaattaata ataaattgta attaaataac aaattaactt
       61 ggcatagatt ataaaatgtt ttaatatata taatctaaat aaaaataata ataaaatgac
      121 ttttaaaatt taaaaaaagt aaggagaaaa ttaattgttc aataaaatag gttttagaac
      181 ttggaaatca ggaaagcttt ggctttatat gggagtgcta ggatcaacta ttattttagg
      241 atcaagtcct gtatctgcta tggatagtgt tggaaatcaa agtcaaggta atgttttaga
      301 gcgtcgccaa cgtgatgcgg aaaacaaaag tcagggtaat gttttagagc gtcgccaacg
      361 tgatgcggaa aacaagagcc aaggcaatgt tttagagcgt cgtcaacgcg atgttgagaa
      421 caagagccaa ggtaatgttc tagagcgtcg ccaacgtgat gcggaaaaca agagccaagg
      481 caatgtttta gagcgtcgtc aacgcgatgt tgagaataag agccaaggca atgttttaga
      541 gcgtcgtcaa cgtgatgcgg aaaacaaaag tcagggcaat gttctagagc gccgccaacg
      601 tgatgcggaa aacaagagcc aagtaggtca acttataggg aaaaatccac ttttttcaaa
      661 gccaactgta tctagagaaa ataatcactc tagtcaaggt gactctaaca aacagtcatt
      721 ctctaaaaaa gtatctcagg ttactaatgt agctaataga ccgatgttaa ctaataattc
      781 tagaacaatt tcagtgataa ataaattacc taaaacaggt ggtgatcaaa atgtcatttt
      841 taaacttgta ggttttggtt taattttgtt aacaagtcgc tgcggtttga gacgcaatga
      901 aaattaagta taatcaatca tttagtaact attataatga tatatacaat caataaaaag
      961 gaatcggata cgagattcct ttttataatt aggttggtta gggtgacttt tttcatttgg
     1021 ctattcttga aagtttataa aaatgtagta taatagtcac attaaaatgt tttgaaaata
     1081 ttgatgaata acatcaacaa atagaggtca ttatatggga tataccgttg ctatcgtggg
     1141 tgctacaggt gccgtaggaa cacaaatgat tcgtcaatta gaacaatcga atttaccaat
     1201 agaacaagtg aaacttttat catcaagtcg ctcagcaggt aaaattttac attttaaaga
     1261 tgaggctata cgtgttgaag agacaacaaa agaatcattt tacgatgttg atattgcctt
     1321 gttttcagct ggtggatcta tttcagcaaa gttcgctcct tatgcagtaa agtctggagc
     1381 agttgtagta gataacacgt catattttcg tcagaatcct gatgttccac tagttgttcc
     1441 tgaagtaaat gctcatgcca tgattggtca taatggtatc atagcttgtc ccaattgttc
     1501 tactattcaa atgatgattg ctttagagcc cattcgtcaa aaatggggga tagagcgtgt
     1561 tatagtttcc acctatcaag ctgtttcggg ttcaggtgca cgtgctgttg aagaaactaa
     1621 ggaacagttg agacaagttt taaatgataa cctttcacct gatcaattaa ttgcaacggt
     1681 cttgccatgt tctagcgatc aaaaacatta tcctatcgca ttcaatgcat tacctcaaat
     1741 tgatattttt actgataatg attatacgta cgaagaaatg aaaatgactt tggagacaaa
     1801 gaaaattatg gaagatgcta ctattaaggt ttcagctacc tgcgtccgca ttcccgtttt
     1861 atcgggtcat tctgagtcta tttatattga gacgaaagaa ttagcctcaa tctctgaaat
     1921 taaaaaggct attgcaaatt ttcctggtgc cgtcctacaa gaccttccat ctcagcaaat
     1981 ttaccctcaa gcgattaatg ctgtaggcca tagggagacc tttgttggtc gtattcgaaa
     2041 agaccttgat caagaaaatg gtgttcatat gtgggtagtt tcagataatc tactgaaggg
     2101 ggcagcttgg aattctgttc aaatcgctga aacattgcat aagaatgggc tggttaaacc
     2161 agcgaaagag ttaaaatttg aattaatata gaagaatatg acattgctta atacttatac
     2221 tgacttctag tgatattaat ttttcatcaa tatcaggtaa aacacttgac aaattatgaa
     2281 aacatttgca taatgaaatt aacaaatgaa aatgattgca aatcgtttta acattgtttc
     2341 cacattaagg aggggctctt atgcaaatca tcaaacgtga tggacagatt gttgaatttg
     2401 atcctgaaaa aatctaccaa gctatcttga aagctgctca aacagtttat gtgcttgatg
     2461 agtcacttcg tcaagacctt gctcaagtga ctaaaaaagt aatcttagat ttagaggatg
     2521 ctcaggttga acgaccaacg atcaatatga ttcagtcttt ggttgaaaat cgtttattgg
     2581 atgcaggata cattcatatt gctgaacact atatttcata ccgtttgcaa cgtgatttag
     2641 agcgaaatgg ttatgatgat caaatcactg ttcatttaca ctttgaaaaa attaagtaag
     2701 gtttgacttt taagtttaaa ctctctatac ttgtatatgg aacttactat gtaatgtgct
     2761 aatttaaaaa cttgtagagt gtctctatga gttttttaat aacctctatt atcaatcaaa
     2821 ggattttaaa tgagtatttt agaagtaaaa aacttaagtc acggttttgg tgatcgtgct
     2881 atttttgaga atgtttcttt tcgcctttta aaaggtgaac atataggact tattggtgct
     2941 aatggtgaag gaaaatcaac attcatgtca attgttacag gtcatcttca acctgatgaa
     3001 ggaaaaattg aatggtcaaa atatgtaact gctggttacc ttgatcaaca tactgtgtta
     3061 gaagaaggtc aaacagttcg tgatgttttg cgaacagcat ttgatgaact ttttaaaaca
     3121 gaggcacgta tcaatgatat atatatatcc atggcagatg atggcgcaga tgtagatagc
     3181 cttatggaag aagttggaga actacaagat cgtttagaat cacgtgattt ttatacgctt
     3241 gatgctaaaa ttgatgaagt cgcacgggca ttaggagtta tggattttgg tatggattct
     3301 gatgttactg aattatctgg tggtcaaagg acaaaggtcc ttttagcaaa actgttatta
     3361 gaaaagccag atatcctcct gcttgatgag cctacaaact accttgatgc tgaacacatt
     3421 gattggttaa aacgctattt acaaaattat gaaaatgctt ttgttttaat ctcacacgat
     3481 attccatttt tgaacgatgt tatcaatatt gtctatcatg ttgaaaatca agatttagta
     3541 agatattccg gtgattatac taattttgaa tcagtctatg caatgaaaaa ggctcaatta
     3601 gaagcagcct atgaacgtca acaaaaggaa atagctgatt tacaagattt tgttaatcgt
     3661 aataaagcac gcgttgcaac acgtaatatg gcaatgtctc gtcaaaagaa actagacaaa
     3721 atggatatta ttgagttaca agcagagaaa ccaaagccaa gttttgaatt taaagaatct
     3781 cgcacaccag gacgttttat tttccaagct aaagatcttc aaattggata tgatcgtgct
     3841 ttaacaaaac ctctaaacct aacatttgaa cgtaatcaaa aaatagctat tgttggagca
     3901 aatgggatag gaaaaacaac attacttaag agtttactag gtattatccc ccctatttct
     3961 ggtaatgttg aacgaggtga ttttattgat ttaggttatt ttgaacaaga agtacccgga
     4021 gggaatcgcc aaactccact tgaagcagta tgggatgctt ttcctgcttt gaaccaagca
     4081 gaggttaggg cagctcttgc tcgttgtggt ttaacttcaa agcatattga aagtcagatt
     4141 caggtgttat ctggtgggga gcaatcaaag gtcagatttt gcttattaat gaatcgcgaa
     4201 aataatgtcc ttgtacttga tgaaccaacc aatcatttag atgtagatgc taaagatgag
     4261 ttaaaacgtg ctttaaaagc ttataaggga tcaatcttga tggtatgtca tgaaccggac
     4321 ttttatgaag gatggatgga tgatgtttgg gactttaacc aacttagtta agtggctagg
     4381 gtaataaagt agaaggcaga gccaaataag atgcaaaatg atatgagcgt tgttcttaga
     4441 gctggtaaat tactgattga aagcggagca gaagtctatc gtgtcgaaga cacaatgaag
     4501 cattttgcta aggctcttca gatagaaaat tttgaagctt atgtggttag tagcagtatt
     4561 atcgcttccg ggattaaccg ctatggtaaa caagaagcta aagtatgcaa tacagatggt
     4621 gtaacagcaa atttaggtag acttgaagct gttaataacc tttctaggca aattgctaaa
     4681 caagacttag tatctccaga agaaattgta aagcagttag acttaattga acatcaaaaa
     4741 gattatagtt tgcttgttac tttaatttct tatttttgtg gagcgggtag tttttcttta
     4801 gccttgggta gttccttatt agattcattt tccgctgctg taacaggctt aattttagga
     4861 tattttttga atttgatgga aagtcgtata catacaggat ttcttttgac cattctaggt
     4921 agttctgtag tggctttaag tgctaattta ttatattttt caggattagg tgagcatcgt
     4981 agcattatca tattaggggc attaatggtt atggtgccag gggctgcctt tgttaattcg
     5041 gtgagagaat tttcacagaa taatttttca acaggtttag ctttgattat gtctgcttta
     5101 ctcatttgta tttctatatc agcaggcgtg gctattacaa ttgaaattat tccgtttgca
     5161 gaacaaatga caggttcttt ttctggtgtt cctaatacca ttcttgagat tatcatcaga
     5221 acactaatgg ccggtttagg gacgatagcc ttctctattc tctaccatgt tccaaaacgt
     5281 tatttccttg acttagggat actaggagca atatcttgga tgctctacct gatcttatgg
     5341 caacaatttc acatggatgc tatagccgta ttttttccag ggcttttcat cacttatttt
     5401 tcacggctat tagctgctaa acgtaagtgt cctgccactg tctttttagc gacaagtatg
     5461 tttcctctta tcccaggact tagcttctat agagccgttt actttttatt gacgggagca
     5521 gatgccttag caatggaata ttttagatcc tgttttgtaa cagcttttac aattgctata
     5581 gcaatcagta ttgtacagca gattcctcta tcgttcttta ttagacgtaa aatgataaaa
     5641 taatttagta tttatttgac aaaatgattg taaacttgta gaatacctgc aaatgtatct
     5701 ttaagtgagt ccagagaggc gaacaaggtc accatcataa gtcaaatagc gagtgctttt
     5761 tcgctaactt tttatgaaac cttgccctca ggcaaggttt tttgtgtcat actggtacat
     5821 ttccaacaat tattatttct atagagagga attatatgtt agaaaaatgc cctatcattg
     5881 ccttagattt ttctgatttg gcttctgtaa caactttttt agaacatttt ccaaaagaag
     5941 aattgctttt tgtcaaaata ggaatggagc tttattattc cgaaggtccc tcaattatta
     6001 gatatattaa atccttagga catcgcattt ttttggattt aaaattacat gatatcccta
     6061 atacagttcg attatctatg tctgtcttag ccaaattagg cattgatatg actaatgtcc
     6121 atgctgcagg aggagtcgaa atgatgaaag cagctagaga aggtttgggg gagggtccaa
     6181 tactattagc agtgacacaa ttaacctcga cttcacaaga acaaatgcag gtggatcaac
     6241 atattaactt aagtgtagtt gattcggttt gccattatgc tcaaaaagct caagaagcag
     6301 gcttagatgg agtagtcgca tcggctcaag aggtaaagca aatcaagaaa cagactaacg
     6361 aacatttcat ttgcttaacg cctggtatca gacctccaca gaccaatcag ttagatgatc
     6421 aaaaaagaac gatgactcct gagcaagcac gtatagttgg ggctgattat atcgttgtgg
     6481 gaagacctat aacgaaagca gaaaatccat atcaagcata tctagacata aaagaagaat
     6541 ggaatcgtat taaataaagg agtactgaga tggatttagc aagacagatt gccatggaac
     6601 tattagatat tcaagctgtt tacttaagac cccaacaacc ctttacttgg gcttcggggg
     6661 tcaaatcgcc aatttacact gataatcgtg taaccttatc gtatcctgag acaaggacac
     6721 tgattgaaaa tggttttgtt aaacaaatac agaaacattt cccaaacgtt gatatcatcg
     6781 caggaacagc gactgcaggc ataccacatg gcgctattat agctgataag atgaacttgc
     6841 cctttgccta tatacgctcg aaggcaaagg atcatggtgt ggggaatcaa atcgagggac
     6901 gtgtttactc aggccaaaaa atggttatca ttgaagattt gatttcaaca ggaggatctg
     6961 ttttagaggc tgttacggct gcgcagagtc aaggtattga ggttttaggg gtggtagcga
     7021 tatttacata tcaattagct aaggctgaac aagcatttcg tgaagctgat attcctttgg
     7081 tgactttgac ggattacaat caattaatta aagttgctaa agttaatgga tatattacag
     7141 ctgaccaact ggtattactg aaaaaattta aagaagatca aatgaattgg caaagttaga
     7201 gaggacagat atgtatatca ttaaaaacgg attgattatc gaccctcaaa gtggttttaa
     7261 tcaagtatca gacatgctga ttgatcaagg aaaaattaaa caaatatcga aagaaattga
     7321 tattaaaggc attccaatca ttgatgcaag caataaaatt gtagctccag gattagtcga
     7381 tattcatgta cattttcgtg agcctggaca gacacataaa gaaaatatcc atacaggagc
     7441 cttatcagct gcagcaggtg gttttacgac agttcttatg atggccaata ctaacccaac
     7501 gatttctagc tcagaaattg taaaacaagt taaggagagt gcagcaaaag aagcaattaa
     7561 gattgaaaca gttgctacaa ttactaaatc cctgaatggt aaagatttag ttaatttcga
     7621 agaattatta gaagctggag ttgctggatt ctcagacgat ggtatccctt tgacagatac
     7681 taaggttttg caagaagcta tgaacttagc tagaaaacat gatgttgtcc tatcgttaca
     7741 tgaagaggat ccatcgttaa atggtgttct tggtatcaac gaacatattg ctcagaaaat
     7801 ttatcatgtc tgtggagcta gtggtcttgc tgaatattca atgattgcgc gagatgctat
     7861 gattgcttac caaacacagg cgaaagtcca tatccagcat ttgtcaagtt cagaatcagt
     7921 agaagtagtt gattttgcgc agaagcttgg tgctaactta acagcagaag taacccccca
     7981 acatttttca aagacagagg acttactttt aactaagggt gctaatgcaa aattaaatcc
     8041 accactgcga cttgaaaagg atagacaagc cctcattgat ggtcttaaaa gtggtgttat
     8101 ttcaattatt gcaagtgatc acgctcccca tcatattatg gagaaagcag cagataatat
     8161 tagtcaagcg ccctctggta tgactggctt agagacatca ttagccttag ggattactta
     8221 tttagtatct actaaagaac tctcaatgat tgactttttg gcaaaaatga catgtaatcc
     8281 tgcacagtta tatggatttg atgcaggtta tctcagagaa ggtggacctg cagatatcgt
     8341 tatttttgat caagctgaag agagaataat aaaggcagaa tttgcctcca aatccagcaa
     8401 ctctcctttt atcggtgata aattgaaggg agttgttcac tacacaattt gcaatggtga
     8461 aatagtctat caaaaagata gccacgcagc gacattggta taggtaaaac atttcttatt
     8521 aatatccttt aaggtattgt ccagagaggc aaacaaggaa ttgcgttaac aaatagtaag
     8581 ttagttccct atctagctta ctattttgca gagcctcctt gttttaggag gcttttttct
     8641 agctaaaatt tatagaaaga aattatcatg aatcatcaaa cacaaacact ttcattagaa
     8701 cattttgtca gtttagaaga attatcaaat caagaagtta tgtctttgat aaagagaagt
     8761 atcgaagtta aagaaaatcc tagcaacatt ggttttgata aagattacta tgtgtcaaat
     8821 ctcttttttg agaattcaac aagaacacat aagtcatttg aaatggcaga actcaaatta
     8881 ggattaaaaa caattgaatt taatgctgat acaagttctg ttaataaagg agaaaccctt
     8941 tatgacacta tcttaacaat gagtgctcta ggattggacg tttgtgtcat taggcaccct
     9001 gatattgact attataaaga attgattgct agtccgaaca ttcattcagc tattgtcaat
     9061 gggggtgatg gctcaggcca acatccaagc caatcacttt tggatttggt aactatttac
     9121 gaagaatttg gatacttcaa aggactaaaa attgcgattg taggagatct aactcactca
     9181 cgggtagcta aatctaacat gcaagtgcta aaacgattag gagctgaaat tttcttttct
     9241 ggacccaaag aatggtatag tagtcagttc gatgaatatg ggcaatgttt accaatagat
     9301 cagttagttg atcaaattga tgtccttatg cttttacgtg ttcaacacga acgtcatgat
     9361 ggtaaagggg tgttttcaaa agaaagctat catcaacaat ttggtcttac aaaagaacgc
     9421 tataaacatt taagggatac agcaattatt atgcacccag cacccgtcaa tagggatgta
     9481 gagattgcta gcgatttagt agaagcagat aaggctcgaa tagtgaaaca gatgagtaat
     9541 ggcgtctatg caaggatagc tatcttagaa gcagtgctca atagcagata ataaaggaga
     9601 taaaatgaag cgtctattat tattggaaga tggtagtgtg tttgagggtg aggctttcgg
     9661 agcggatgta ggaacaagtg gtgaaattgt ttttagtaca ggaatgacag ggtatcaaga
     9721 atctataaca gatcaatctt acaacggtca aatcattact tttacttatc ctcttattgg
     9781 aaattatggg ataaatcgag acgattacga atctattaga ccaacatgta agggagtaat
     9841 tgtttatgaa tgggcagagt atccaagtaa ctggcgtcaa cagatgacat ttgatgaatt
     9901 tttaaaatta aaaggaatac ctggaatttc aggtatagat actcgagcat taacaaaaat
     9961 tattcgaaaa catggaacaa tgaaagcttg tttaataaac gagggaaatt ctattcatga
    10021 agcactagaa aaccttcaaa aaagtgttct tttaaataat caaattgaac aagtatcaac
    10081 caaattagct tatgcttcgc ctggagttgg caaaaatatt gtattagttg actttggttt
    10141 aaaacattct attctaagag aactgtcaca acgtcaatgt catattacag tcgtccctca
    10201 tactacgaca gcgcaagaaa tcttaaatct caatccagat ggagtactct tatctaacgg
    10261 ccctgggaac ccagaacaat taccgaatgc tttacaaatg atccaagaaa ttcaaggtaa
    10321 aattccaatt tttggtattt gtatgggaca tcaactattt gctaaagcca atggtgcaaa
    10381 gacttataaa atgacttttg gtcatcgagg ttttaaccat gctgttcgtc atttgcaaac
    10441 aggacaggtt gattttacaa gtcaaaatca tggttatgct gtctcgagag aagatttccc
    10501 tgaggctctc ttcattacac atgaagagat taatgataaa accgtcgaag gtgttcgaca
    10561 taaatactat cctgcctttt cagtgcagtt tcacccagat gcagcaccag ggccgcatga
    10621 tacatcttat ctttttgatg aatttattaa tatgattgat gatttccagc aaaaaagcta
    10681 gctttaaaat atagaattag aggagtttga tatgccaaag cgcacagaca tacgtaaaat
    10741 tatggttatc ggttctggcc cgattgtgat tggacaagca gcagagtttg actattctgg
    10801 tacacaagcc tgtctctctt taaaagagga gggataccaa gttgttttgg tcaattcgaa
    10861 tccggctact atcatgacag ataaagatat tgctgataaa gtttatattg aacctattac
    10921 cttggagttt gtcacaagaa ttctcaggaa ggaaagacct gatgctctcc ttccaactct
    10981 tggaggtcaa actggattga atatggctat ggctctatca aaaaatggta tcctagaaga
    11041 attgaatgtt gaactattag ggaccaaatt atcagccatt gataaagctg aagatcgtga
    11101 tttatttaaa cagttgatgg aagaactcaa tcaacctatt ccagaatccg agattgttaa
    11161 ttcggtagaa gaagctattc aatttgcaga gcaaatcggg tatccattaa ttgttcgtcc
    11221 tgcctttact ttaggaggta caggtggagg catgtgcgat aatcaagaac aattggttga
    11281 catcacgacg aaggggttaa agttatcgcc tgtgacacag tgccttattg aacgttcaat
    11341 tgctggcttt aaagagattg aatacgaagt tatgcgtgat gcagcagata acgctcttgt
    11401 tgtttgtaat atggagaatt ttgatcctgt tggtatccat acaggggact ctatagtttt
    11461 tgcacccgca caaacgttat ctgatgtgga aaatcagtta ttacgagatg cgagtttaga
    11521 tattataaga gctttgaaaa ttgaaggagg atgcaatgtt caactagccc ttgatccgaa
    11581 tagttttaaa tactatgtca ttgaagttaa tccaagagta tcaagatctt ctgctttagc
    11641 ttcaaaagct actggatatc caattgctaa attagcagct aagatagctg ttggtttgac
    11701 actagatgag gtaattaatc ctattacaaa aacgacttac gctatgtttg aacctgcttt
    11761 agattacgta gtcgcaaaaa tgccgagatt tccttttgat aaatttgaaa gtggcgatag
    11821 aaaattggga acacagatga aagctacagg cgaagtcatg gcaataggac ggaatataga
    11881 agaatctttg ttgaaagctt gtcgttcact agaaataggt gtcgatcaca ttaaaatagc
    11941 agatttagat aatgtatcag atgatgtttt attagaaaaa ataagaaagg cagaagatga
    12001 tcgcttattt tatctagcag aagctttacg tagacattat agtattgaaa aattagcaag
    12061 tttgactagc attgattctt tcttccttga taagttaaga gtgattgtag agttagaaga
    12121 tctattatct aaaaacagac tggatatcaa tattctcaaa aaggttaaaa ataaaggttt
    12181 ttcagataaa gctatagcga gtttatggca aataaacgaa gatcaagttc gtaacatgcg
    12241 aaaagaagca ggaattcttc cagtttataa gatggttgat acatgtgcgg ctgagtttga
    12301 ttcagcaacg ccctactttt attcgactta tgccgtagaa aacgagtcat tgatatcaga
    12361 taaagcctct attttggttt taggatcggg cccaatccga attggacagg gagttgaatt
    12421 tgattattca acggttcatt ctgttaaagc catcagagag tctggttttg aagcgattat
    12481 catgaactct aatccagaga cggtctcaac ggatttctct atttcagata agctttactt
    12541 tgaacctttg acttttgaag atgttatgaa tgttattgac ttagaaaaac ctgaaggggt
    12601 catcttacaa ttcggtggtc agactgctat taatctagca aaagatttaa acaaggcagg
    12661 ggttaaaata ctaggcacac aattagaaga tttggatcgt gctgaaaata gaaaacaatt
    12721 tgaagccact ttacaagctc taaatatccc tcaaccacca ggttttaccg ccactacgga
    12781 ggaagaggca gttaatgcag cgcaaaagat tggttatccc gtgttggtta gaccatcata
    12841 tgttcttggt ggacgagcta tgaagatagt tgaaaacgaa gaagatttac gtcattatat
    12901 gacaaccgcg gtaaaggcaa gcccagatca tccagtgctt atagatgcat accttatagg
    12961 taaagagtgt gaggttgatg ctatatccga tggtcagaat atactgattc ctggaataat
    13021 ggaacatata gagcgagctg gtgttcattc aggagattcc atggctgtat accctcctca
    13081 aacattgtct gagaccatta tcgaaactat tgtagattat acaaaacgct tagcaattgg
    13141 attaaactgt atcggtatga tgaatattca gtttgtaatc aaggatcaga aagtatatgt
    13201 tattgaagtt aatcctcgtg ctggtagaac acttccattc ttatcaaaag taactcacat
    13261 tcctatggcc caagtagcaa caaaagttat cttaggagat aaactctgca attttacata
    13321 tggttatgat ctctatccag cttcagatat ggtccacatt aaagcccctg tctttagttt
    13381 taccaaactt gctaaagtag atagtttatt aggtcctgaa atgaaatcaa caggcgaagt
    13441 gatgggatca gatattaatc ttcagaaagc cttgtataaa gcctttgaag cagcatatct
    13501 ccatatgcca gactatggta acattgtttt tacagtagat gatacagaca aagaagaagc
    13561 tttagaactt gcaaaagtct atcaaagtat tggttatcgt atttatgcta cccaagggac
    13621 tgcaatttat tttgatgcta atggtttaga gacagtttta gtaggtaagt taggagagaa
    13681 tgatcgaaat catattcctg atttaattaa aaatggtaag attcaagcag ttatcaacac
    13741 agttgggcaa aataacattg ataaccatga tgctctcatc atcagacgtt ctgcaattga
    13801 acaaggagtt cctctattta catctttaga tactgcacat gcaatgttca aagttcttga
    13861 aagcaaagca tttacattaa aagtactaga ttaatcaaac aatcaaggta aaattaactt
    13921 ccagcaaaac agaatcggac tagattaaat atagtttgtt ctgtttttat aaaaaagcct
    13981 aattgtgata aaatagggaa caaacttaag gagttaatta tgttaaaaac acgaacacat
    14041 attaatgaat tagaaacaaa aatcgccctt gatttagtac acagatatct aggtattcaa
    14101 caacttgttt tttcaaagtg gagacattct ttagaaactg gttcatggtc ttctattata
    14161 acaataaatg gcgctaatat tatcaattac attgttgatg atattagcga cagtgatagt
    14221 atttcaggta ttatttgtca tcctaaaagt tttcatctca gacctagaga aaaaaaggaa
    14281 cctattcagt ctattgatga gttagatatc atctggtctt tgaatagaga ggaaaatgaa
    14341 tgattccaga tagtagtttt aaagtgctat cagatatggc atataatatt gaaaaatcca
    14401 aagcagaaag taatggacac cttaaaatta tagaaggtag tcaaattatt gatttccatc
    14461 aaaaacgctc ttttatggta ttagaggtta atgataatat cgataatggt atgcaggcta
    14521 tggcatgtca gtctttggat gagagtaatc atattgttat agcatattca ggaacgaacg
    14581 cttttaataa gaaagatatc ttaacagata tcgattcagt tgtattgggg aagaacaata
    14641 aattgctaac ttgtccttca agtattaccc tgagagagga tggcatttct ctgccttgta
    14701 aaaatccaag tcaaattaca acatctaaac tatttgcaca aaaaattaag atgaactatc
    14761 ctgatgcaac cctctggtta acaggccatg gtaaatgttc agtcaggtaa tatctcaaat
    14821 taccaaaaaa atcttttaga ttctgcaact aatttccaaa atatctttcc agccctcgtt
    14881 aaccagtata gtagttccat tactgctaac gaatcactga aaaaatcttt agaagcttct
    14941 gataacatgt ttaatgattt gatgacaacc cagacaaata ctggaaaaga tttatcaagc
    15001 ttaatagaac agcgccatca agatagcatt tcgtatgaag cgttttcggc ctcattacta
    15061 gaaatgaata acgagttatt agagaagcaa ttatctgata ttatcacaca agcacaaaaa
    15121 gaccaagaaa cattatcatc acaactcaat agtattatgg gtgatgataa cgataatgat
    15181 cataatcata aagaaaactc atcagcttat ctaaatgttg caaggcaaaa aatccaagaa
    15241 ctatctgaag cactcaagtc acaagataac atcgctaaag atcaaagtga acaactagat
    15301 aaaattgtta gagagggact agcaagttac tttgctaaga ataataaaga taatattact
    15361 ttattagaat tattgaagag tcattctact aatgagaaga ctttgaaaga ttttaaagtt
    15421 aaggtagcag atttcacaaa ttctctcatt tcaagcattc cctcactaaa cctttcggag
    15481 ttgcacttaa cccaagaaga aaaagccatt cagtttacat cttctgattc ggaaatcatt
    15541 aaaaaagtaa gcaatgaaag aactctgtat ttaaatacta atttattgaa tcgtcttttt
    15601 gaagctagga aaaatagaga tgaagccaaa aataaagtta atcaattaaa attgccatct
    15661 agttctactc gaactggtga gcagatagtt tctgttgaat caaataatcc agattacagg
    15721 gtagatactt ggacggttaa tggtaagcaa acaagaacct tggaccccag ccaaaacaat
    15781 aatattatta tcaatagcag ttatcaaggg gaattatcta actctagtat tacaaaacca
    15841 agctatacaa tcactatcgg gaatcagaaa caaattgtac aaaatgatga cggtgaaatt
    15901 caacggtctt acttggaggc agaagcaacg tatcaacgaa ctctacaaga agtaattgat
    15961 gcatataaca ccactcacga attagtagct aaatactaca ttatttctga tggcgaagaa
    16021 ccgcaaaatt tatttgatca atttttaaat caaagtgtta acgatacgat ggttgatctg
    16081 gtcaaaaatg gtataactaa gtatttgatg gatgaaaaca ctgctgatgc gcaacagaaa
    16141 gttaaagatg tcatggagga agttgaaaat agccaagatg aattggcgga tcaaatggca
    16201 aaagtaactg agacaaatgt acgactaaca gatacaatca aaaaacagct tgagactctt
    16261 caaacaatta acatgaaggt ccaaaacatc acacaggatc aatctaaagt aaacgactca
    16321 cagaaaacaa ctgatcaaca attatctgat ttgaagaacc agctagatgg gctaatgacc
    16381 tctgcaactg gcgtaaaaga tatgtccaaa tctaatagtc aagaggcgga ccaagttaat
    16441 caaatattca catcattcaa taaagatgtt caagatgcta aaaattcggg caacaaactt
    16501 tcgacggatg caacagattt aatggctaac ttccaaaaag aattggccaa taacggtgat
    16561 ttcgtagctt ctttctctaa agtttttaat actgcttata aaaatggagt gccaaatgat
    16621 attctcctta atttcttatc acgacctgtc gctgaatcag cgtctgcagt tagggcaact
    16681 gaaaatactt atcgcccatt cacttggata ctattattag aagttgttag cctatttata
    16741 gcatatatct ttgcgactca aaaccttatt aagaaattga cagataggta taatgtcaat
    16801 cgctggcttc aaacagactt tttaaatatc attgtcattt caggcctctc tcttgtgatt
    16861 ggtttagcac tgggagttat ctcaagcaga agtcttcatg tcatgcctga atatgtacca
    16921 tcttggttcc tagttatgac tctgtttagt tttttattaa ttcatagtca gtatttcttt
    16981 attaaaaatt ttaaagcagt tggtatgggt ttagctttgt ttatgattat tagttttgtg
    17041 tatctatcaa atgccgtagg cacagtagcg actgttagtg gatttccaaa attactaaaa
    17101 gctattaatc cactatctat ccttgaaaat caattatcat cttattttga taatgtaaca
    17161 actggattta ttttccttat cttagtactt cttgtggatg ttgcttttat tatcatgaat
    17221 atttttatca ccttaaattt tgaagctaaa gtaaaagagt gaggttgttg atgaagttga
    17281 aacgattttt agcctgtcta ctaatattat cctgtatgag tcaaacatca gtattttctg
    17341 atgggttaaa gaataatgat attcattttg atacaaaacg tttagaatca gattcacaaa
    17401 aaaattctgg ttttcaaact gaattaacta aagatttatt taaaaaagaa gaccttaaaa
    17461 aattggataa aaaccgtcaa tctaaagtct cactccagaa aaagcagtct aagcaactgt
    17521 ttcaatattc aaataaagga caatctgata ggaaaaaacg tatcaaacaa ttattttctg
    17581 aagaaagtaa tcaatttaaa tttgttacaa gaacagaaga agcgactgtt tcggctgata
    17641 ttggtcatat gattatgtac ctggttctca ttagtttggt actaatatta gcttctataa
    17701 tgacttttaa aataaaggac aaaaaggatg aatacacata ttaacattac tctagatatg
    17761 ggggaaacct caaaagattt acgtatccct ttgaaaatta gcgttaagca attagtaatg
    17821 gaattagata gtatttttag ccgtactgaa aggcgtacta aatatcaact aagagttata
    17881 aataaaggta ttttattggg agagaacgat atattagcgc accatccagt aacaactggt
    17941 gatcgtataa ggatagagga gttttattaa tggaaacaga ttcgtttgta tttgaagaac
    18001 aagactttca atttgaaaaa ggaccagatt tttggcagtt atctcttaag cgttcaaaag
    18061 taggaactca aaatctgcaa caactgcaat tgttagaaat tcaccattct ttgttgatgc
    18121 ctatgacaac tgcaattaat gcagatacca ttcaatttca attccagaca gaagcgcacg
    18181 ctctatcttt tgaaagcttt aaaaaagaaa ctttatctga aaaattacga ttagccttga
    18241 atgtcttgga tttagataag gctttgtctt tgccagtaaa tttcattttg catccctcta
    18301 atctattttt aacaaaaaat gcaactgcta aaatagctta tcgtagtctt cctgggatta
    18361 tgacacctga gaagtttggt ccagaggagt ttttatatca attcaaatgt tttgtctttg
    18421 cattactgac gcaacatgac tatatagaat tgtataatgg tgctatttct gttattgaag
    18481 tatcagattt cctaaaaagc atttatcatg cagaaactat tcaggctgtt agagacatca
    18541 ttactactga ttatgagcag caagtagaag tagaaactca tacgttagca aaagtatcaa
    18601 aggcaaaata taagctttat aaatacataa gtgtctggct aggagcttta tctacaatac
    18661 tcttgattcc gctagtatat ctggttttta ttcacaatcc attcaaagaa aaaatgttgg
    18721 cagcggatac ttcatttatt aaagttgatt ataatcaagt gattaatcga ctagaacatg
    18781 taaaagtaag taagttacct tatacacaga agtacgagtt ggcctattcc tatattaatg
    18841 gaatgtcatt ttctgaagaa cagcgtgaag ttattttaaa caatgttacg ctcaagactg
    18901 atgagctcta tcttgattat tggattaata ttggccgtgg tttagatgat gatgctattg
    18961 atgctgccaa acgtttagac gattctgacc ttgttattta tgctattgtt cagaaaatgg
    19021 atcaggttag aaaagacaat agtttatctg gtaaagatcg tgaacaaaaa ctttctgagc
    19081 tacaaacaga ctatgataaa tattggaaag accgtaagac agccttaaca gatgaggaat
    19141 ccaaatcgaa aaatagcaat aatcattcga caaattccaa caaagagtca tctgaatcat
    19201 caagtactac agcaagtaca tcttctaaaa ctaaaagtag gtaggtggat atgttagtta
    19261 tttgttatta tcaatcactc cgctacgaat ttaatcttga agaggagaaa tcctttctaa
    19321 tatcatcaaa cggcaagtcg ccaataccag tatcagattt agaaaatgac atcacgctaa
    19381 aaaatatgca aggtcaattg gtatatatta ttgaccaaaa agaaaaagag ttaactaatg
    19441 gtgtagagat ttcaggaatt gtcttctatt tagcgaataa tcaaaaagaa atctatactc
    19501 ccttagatta cgaagatata ttaatcggtg ataaagaagg gtatcatgtc cgttttaaag
    19561 aaggagcccc taatttatta ctgaaaaaaa tagaaagcaa ttggcagctt aatttatttg
    19621 aaggtgatat ttatctgaat aatcatcttc aaaaagtagt gcagcagtta cctttaagtt
    19681 taggtgatga gattagcttc caaggaacaa ttgttaagct tttcccagat gaaattcaaa
    19741 tctggggggg gggactgatt atgaaacatc attaactaag aaagttatgt cagcttatca
    19801 attttacgaa ggctatcctg attttcaccg ctcacctcgt attatttatc gttccagtga
    19861 agataaaatt acagttaatg cgcctggtaa taaacctaat aagtctaaag atgaactgtt
    19921 aaaattaatt gttcctcctc tagtgatgat tggagtcagt attcttattt ctatttttcg
    19981 tcctcgtggt atctatatca ttgcaacaat gtcaatggct ctagtgacga tgattttctc
    20041 tatcactggt tactttaaaa atcgtaaaca gtataagcag gacttacaag aacgcattga
    20101 ttcctatcat gactatttat ctgataagtc aattgaactt cagaagttag ctaaagagca
    20161 aaagcgtggg caacactatc attacccaac gattgaaggt ttacaggaaa tggctgatac
    20221 ttatcaccat cgtatttatg aaaagacacc acttcatttt gacttcttgt attatcgcct
    20281 aggtttggga gaagtaccaa cgagctataa tattcattat tctcaaccag aacgttctgg
    20341 gaaaaaagat cctttagaga acgaaggcta taacctctac tttaacaatc gttatattaa
    20401 aaatatgccg attgttgcga atttatcaca tggaccagtt ggctatatag gaccacgagg
    20461 tttagtattg gagcaactcc aattgatggt taatcaatta gcttttttcc attcttacca
    20521 tgatgttcaa tttattacta ttgttccaga ggaagagatg gataagtggt cttggatgcg
    20581 ttggttgcca catgcaacct tacaagatgt caatgttcgt ggctttgttt ataatcaaag
    20641 aagccgtgat caagtgctca acagtcttaa ccaaatttta aaattacgtc gcacacaacg
    20701 cgaagataaa tctgccaaag aagggacttt attttctccg cattatgtcg ttattgtaac
    20761 tgatgaaaag ttaattttag atcacgtcat catggaattt tttacggaag atcctacaga
    20821 attgggatgt tctctcatct ttgttcaaga tgttatgagt tcactttcag aaaatatcaa
    20881 aaccattatt aatattaaag accgtaatac tggtcagtta gttatagaag aaggagagct
    20941 taaagaaaca gattttgaat tagatcattt cttagaggat tatgacaagg aaaatatttc
    21001 acgtcgccta gctccgttaa atcacctaca aaatcttaaa tcttcaatac cagaagcagt
    21061 tacttttatg gaaatgtatc aagcagaaga atttgaagat ttgcatgttc aagagcgttg
    21121 gataagccat gctccgtata aaagcttagc tgttccactt ggcttacgtg gtcaggatga
    21181 tattgtttat ctaaacctac atgaaaaagc tcatggtcct catggattag ttgcaggaac
    21241 aacgggatca gggaagtcag aaattatcca gtcctatatc ctttctttag ctgttaattt
    21301 ccatccacac gatgtagctt tccttctcat agactacaaa ggtgggggaa tggcaaatct
    21361 ctttaaggac ctgccacact tacttggcac catcaccaac ttagatggtg cccaatcgat
    21421 gcgtgctttg gtttctatta atgctgaatt gaaacgtcgt caacgtttat ttgctaaagc
    21481 tgatgttaat catattaacc agtatcagaa gaaatacaaa cttggagaag tttcagaacc
    21541 aatgcctcat ctcttcttaa tctccgatga gtttgccgaa ttgaaatcca accaacctga
    21601 gtttatgaaa gagcttgtct caacagcgcg aattggtcgt tccctaggga ttcacttaat
    21661 cctagcgacc caaaaaccat ctggcgtggt agatgaccaa atctggtcaa actcacgctt
    21721 caaactagct cttaaagtag cagaccgcgg cgactccatg gaaatgctcc ataccccaga
    21781 tgccgccgag attacacaag caggacgtgc ctacctccaa gttggtaata atgaagtcta
    21841 tgaaatcttc caatcagcat ggtcaggagc agattaccaa cctgaaaaag acgaccaagg
    21901 gattgaagac cataccattt attccatcaa cgaccttggc caatatgaaa tcctaaatga
    21961 tgatctttca ggcttagacc aagccgaaaa tattaaagaa gtacctacgg aattagatgc
    22021 catcgttgaa aacattcaaa ctttaaccaa agaaatgggc atctcagact tacctcaacc
    22081 gtggctaccg cctctaagca atcagattgc agtaaccgat ctccgtaagg aagaatcagt
    22141 agatctctgg agtaaagccc catcttacaa agttgttctg ggctttatgg atataccaag
    22201 ccaacaagca caagaagtag cttaccacga ttttgaagat gatgggcatt tgtcaatttt
    22261 tgcaggtcca agtatgggga aatccacagc ccttcaaact gtaacattgg acttagcacg
    22321 tcacaatagt ccagaatttt taaaccttta tctctttgat tttggtacca atggcctctt
    22381 accattaaga cgtttgccac atgtagcaga tttctttacc attgatgatg acgaaaaaat
    22441 agccaagttt atcagcagaa tcaaaacgga aatgtctgac cgtaaaaaag ccctcagtcg
    22501 ctacaatgtt gcaacagcaa aactttaccg ccaggtatct ggggaaacca tgcctcaaat
    22561 cctgattgtt attgacagtt atgaagggct acgggaagct caaacactga ctaacttaga
    22621 agcttgtttc caaaatattt cgcgtgatgg gtcgtcactt ggcatttcct tagttatctc
    22681 agctggtcgt atggcagctt tgaggtcgtc cttaatggca aaccttaaag aacgtatcgc
    22741 tcttaaatta acagatgact ctgaatcacg gacacttgtc ggtcgtcacc aacacatcat
    22801 ggaagatatt ccaggacgcg gattaatcaa acgtgatgac attgaagtgt tacaagtggc
    22861 tctcccaaca gaaggcactg aaacctttga catcatcaat aacatccaaa acgaatctga
    22921 cgccatgaac agcaaatgga ctggaccaag accaaaagca ataccaattg ttccagaaac
    22981 acttaccttt gaggagttta aggcatatga ggatgttaaa aaagcaagtg agactaatca
    23041 gatttcactt ggtattagct taaaagaagt tgataattgt agcattccac tttcgaaatt
    23101 caaacagtta ctttttactt ctgacaatcc agataatttg tctaacttga cgcgcttctt
    23161 agttaaatct gtttcattga ttaaagatag taaggtttat cttttagaca ctgcttctga
    23221 ttatgatgat ttgacagaac aacaaattga atatatcaat aatcgaagtg aaatagcagg
    23281 acatttagaa gctattgttt cagaaatcta tttaagagaa gaatctggta aaatagaacc
    23341 atggtttgtt attattcctg atatgaatag ttttgtggca gaaacaaata tggataaaga
    23401 acagatttta ttcttacttg actcggctca caaatatcat atttatttct tatttggtgg
    23461 cttagcatca taccttatga ctaatatctc agatgttcca aaagctatta gaacacaggc
    23521 acagtatgtt ctcttgggaa tgagagttat ggatcagtcg gtattaccga aaacatataa
    23581 ttcaaaagaa ccttatttga aaccagatat gatttatatt catgatagaa gacaagagac
    23641 aatgcttaaa atcactcaag aaatagaaat ggagcattga gcatatgaaa aaacaaagac
    23701 tattactgct ttttggaggc ttattaataa tgataatgat gacagcatgt aaggattcaa
    23761 aaatcccaga aaaccgcacg aaaaaggaat accaggcaga acagaatttt aagtcatact
    23821 ttaaatatat atcagataaa aataactatt tagataatat aaaagtttat tacttttcta
    23881 taagtatttc taaagatgta caagataaag tcagtgaaac aacaacttgt tcatatagac
    23941 tagaaaagca aaagaatcaa gagttcattg gtaattttga acatgaagtt agtgaatcta
    24001 gtcaatattc aacc
//
LOCUS       DATROQ010000023        23779 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.023, whole genome shotgun sequence.
ACCESSION   DATROQ010000023 DATROQ010000000
VERSION     DATROQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23779
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.023"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            272..889
                     /locus_tag="VBC94_000824"
     CDS             272..889
                     /locus_tag="VBC94_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979106.1"
                     /translation="MRKFFQDVIVEVAYYLEIALSVILATAIIFFSLALFHDLFSMLS
                     VDQNVDKLFQTFLARAMTIAVGIELIKMFSKPSPDTLIEVLLFALARQLVIEHGSVSD
                     YLMGIASVAILFAVRKYLFTHFDESSNIVLRANNKVKKANILARVQLPCQPNETLGDL
                     LVRHLNDEEKDVAIGSVVYINNVALRVDSMHDGLVTRVEIIRSLH"
     gene            1057..2496
                     /locus_tag="VBC94_000825"
     CDS             1057..2496
                     /locus_tag="VBC94_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6979107.1"
                     /translation="MENFLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPLHDVFAG
                     FIKATVGYLILNVGAGGLVNTFRPILVALAKKFHLEAAVIDPYFGLASANAKLETMGF
                     ISVATTALLIGFGINILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQLRNG
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGGILAVLGPDIMSNVKLIGPGAFVPTKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVTELTNAFQGISNKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVVFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLAGGYHGNIDFEFPWLAFGYIFKYLGIAGYVIV
                     CLFFLAIPQLQFMKSKDKEAYYRGDAPAE"
     gene            2524..2802
                     /locus_tag="VBC94_000826"
     CDS             2524..2802
                     /locus_tag="VBC94_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6979108.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVSNFESASCSVGEA
                     KGLAANYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEIL"
     gene            2869..3354
                     /locus_tag="VBC94_000827"
     CDS             2869..3354
                     /locus_tag="VBC94_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6979109.1"
                     /translation="MNLKQAFIENDSIRLKLSASDWKEAIKLSIDPLIESGAVDAEYY
                     DAIIESTEEFGPYYILMPGMAMPHARPEAGVKRDAFSLITLTEPVVFPDGKEVSVLLA
                     LAATSSAIHTSVAIPQIIALFELENSIQRLTECQEAKEVLAMVEESKNSPYLEGLDLE
                     S"
     gene            3467..4132
                     /locus_tag="VBC94_000828"
     CDS             3467..4132
                     /locus_tag="VBC94_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEO6979110.1"
                     /translation="MTKRLPNLQVALDHSDLQGAIKAAVSVGHEVDVIEAGTVCLLQV
                     GSELVEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKEERGDRGEIQIELYGDWTYEQAQQWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLSTDTLQLFEGVDVFTFIAGRGITEADDPAAAARAFKDEIKR
                     IWG"
     gene            4136..4999
                     /locus_tag="VBC94_000829"
     CDS             4136..4999
                     /locus_tag="VBC94_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEO6979111.1"
                     /translation="MTRPIGIYEKATPKHFNWLERLQFAKELGFDFVELSIDESDERL
                     ARLEWSKEERLELVKAIFETGVRVPTITFSGHRRFPMGSNDPEKEARAMDMMKKCIVF
                     AQDIGIRNIQLAGYDVYYEEKSPETRARFIKNLRQACTWAEEAQVILSIEIMDDPFMN
                     SIEKYLAVEKEIDSPYLFVYPDTGNVSAWHNDLWSEFYNGHRSIAALHIKDTYAVTET
                     SKGQFRDVPFGQGCVDWEEMFAVIKKTNYNGPFLIEMWSENCETVEETRAAIKEAQDF
                     LYPLMEKAGVK"
     gene            5001..5717
                     /locus_tag="VBC94_000830"
     CDS             5001..5717
                     /locus_tag="VBC94_000830"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEO6979112.1"
                     /translation="MAKSLQEMRERVCEANKSLPVHGLVKFTWGNVSEVDREAGLIVI
                     KPSGVDYDQLTPENMVVTDLEGNIVEGDLNPSSDLPTHVQLYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLSEDEVNTAYEKETGSVIIEEFERRNL
                     DPMAVPGIVVRNHGPFTWGKDPAQAVYHSVVLEEVAKMNRFTEQINPRVEPAPKYIMD
                     KHYLRKHGPNAYYGQKGQEH"
     gene            5728..6375
                     /gene="fsa"
                     /locus_tag="VBC94_000831"
     CDS             5728..6375
                     /gene="fsa"
                     /locus_tag="VBC94_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO6979113.1"
                     /translation="MKYFLDTADVSEIRRLNRLGIVDGVTTNPTIISREGRDFKEVIN
                     EICQIVDGPVSAEVTGLTCDEMVTEAREIAKWSPNVVVKIPMTEEGLAAVSQLSKEGI
                     KTNVTLIFTVAQGLSAMKAGATFISPFVGRLEDIGTDAYALIRDLRHIIDFYGFQSEI
                     IAASIRGLAHVEGVAKCGAHIATIPDKTFASLFTHPLTDKGIETFLKDWDSFKKK"
     gene            6538..7521
                     /locus_tag="VBC94_000832"
     CDS             6538..7521
                     /locus_tag="VBC94_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6979114.1"
                     /translation="MFEKVTISDIANLVGVSKATVSYYLNGNYKKMSLQTKEKIRLAI
                     KETGYQPSKIAQSLVTKNTRTIGVVIADITNPFISSVMKGIHDTCQQFGYSVNFTNSD
                     NDIDIELENLNRLNQQNVSGIILDSVDPNHSFIETLSNDRLVMVDRQAKDIKVDTVVS
                     DNKESTQIFLEKMQETGYHDIYFVTYPIEGISTRELRYEGFKEVVSSNPDKLIIITED
                     GSTQRILDIIEHSEQKPGFLMMNGPTLLNFMKKLNQSTVSYPEDYGLGSYEDLEWMQV
                     LTPNVSCIKQDSYGIGCLAAQCLIEKISQGNEPTTARLLEVKNQIVIRQSF"
     gene            7756..8775
                     /locus_tag="VBC94_000833"
     CDS             7756..8775
                     /locus_tag="VBC94_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979115.1"
                     /translation="MKINKLLSDRLEACYSVCRLQNQDYDFLLVASEVDQACFAYDLN
                     SDLAKIVVWEDVGGTMSIIPIPGTMDFLATQKFYPGFKAKDCQVVYASFQGDHWDICN
                     AFDFPYLHRFDLIKGKENQLYFIGCTIANSKAYTEDWSDKGRIFVARFNTQNHTLEGL
                     QQLPQTLLKNHGYYAIQDEGYSLITSVEGVLKLTYPEFSTTGDWQLERLFDEETSDVV
                     KVDINRDGKDEYVIIQGFHGDRLRIFTEDFGRELFHYPEKTPFGHAIWSGRLLNQTCF
                     VFGWRSEKAELRLFHFVDGHLVSELVDAKAASSNVLAFEKDGKAYLFSANNGRGEVAL
                     YQLVK"
     gene            8794..9750
                     /locus_tag="VBC94_000834"
     CDS             8794..9750
                     /locus_tag="VBC94_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO6979116.1"
                     /translation="MDKKKILVTGIVPKEGLRKLMDRFDVTYSEDRPFSRDYVLEHLS
                     EYDGWLLMGQKGDKEMIDAGENLQIISLNAVGFDHVDTAYAKEKGIVVSNSPQAVRVP
                     TAEMTFALILAASKRLAFYDSIVRSGEWIDPSEQRYQGLTLQGSTLGIYGMGRIGLTV
                     ANFAKAFGMTVVYNDVYRLPEDKEKELGVTYLEFDQLIETADVITIHAPALPSTIHKF
                     NKDVFAKMKNRSYLINAARGPIVSEEALIEALKEGEIAGAGLDVFENEPQVSEGLRSL
                     DNVIMSPHAGTGTIEGRRTLAEEAADNIIAFFDGKPQNIVNK"
     gene            9848..11299
                     /locus_tag="VBC94_000835"
     CDS             9848..11299
                     /locus_tag="VBC94_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO6979117.1"
                     /translation="MLEALHKFFEVFGAATVVPAMIFIVSLFLKVNPRRAFFSALRAG
                     VGLTGFGWIISAFAPMVTKYIQQMVKTTGLHLPIVDIGWQAGSLTAFSSEIGLSFFVF
                     GLLIELGLFLLGITRVFVPSNLWNNFGYMIWGTMAYAATGNFILSFAFMVFVLLYSLV
                     MSEVLADRWSEYYGVKNATINSIHNIETLIPALILDPLWNLLGVNKVKLNPESLKTKL
                     GIFGEPMTLGFILGVIIGVLGSLRNLASIDTWGGILGFAVALAAVMTIFPLITGVFAS
                     AFAPLAEAVERNKKKESQAEQGALDKKRWFIAVDDGVGFGEPATIIAGLILVPIMVVI
                     SLILPGNEALPVVDLIAIPFMIEAMIAVSKGNILKAILNGIIWFSLGLYAASALGPIY
                     TEAVKHFGTALPAGVTLIMSFNLLAHPLTALVFFAWISGNPLYIGVTVIIYLAFLFLL
                     RTRRESIYAYLKTMADKNSGYEGSCEKIFIAED"
     gene            11321..12310
                     /locus_tag="VBC94_000836"
     CDS             11321..12310
                     /locus_tag="VBC94_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO6979118.1"
                     /translation="MKKIDGHAHIVDTIAGFNGKGRLNALGNGYAIWDDGDLIKLIPD
                     EYGDKAFTAESFLKYMDANGVDKAVILQGHLNGYQNYYTHLAIKRYPERFTGAFSVDP
                     FADNAMQIVKRHVEVLGFRAIKFEISQGGGIHGYRGQKTPFRLDTDPHVSRILTYLLD
                     YPGFVVTVDYGNWDQISHQPDAIANLARLYTSLDFVVCHLSFPHVEHSNRLRAELNMW
                     KNFNNIYTDISAIQDIDAPDSFPFPKSEQNVRIAKEVLGAKRIIWGTDSPWSATFNTY
                     EELATWLENVDIFSQEELEDVMYNNAERVYFKEEHVKANRAGIDDTTKELNLY"
     gene            12376..13893
                     /locus_tag="VBC94_000837"
     CDS             12376..13893
                     /locus_tag="VBC94_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEO6979119.1"
                     /translation="MTYYLSIDYGGTNTKALIFDKLGHQIAVSSFETLKNEAQSGHRQ
                     VNLVKTWNAITSAIREVIQISKLSPEQISAVACIGHGKGLYLLDNKLEPLEQGILSTD
                     NRAKDLAQYFESKLDNIWELTRQHIFPSQSPVILRWLKDYQPETYKSIGAVLSAKDFI
                     RYKLTGKVQQEYGDASGNHWINFQTGTYDPAILDFFGIREIENSLPELIDSADLVPGG
                     ISSQAAKETGLVEGTPVVGGLFDIDACALGSGVLESDTFSVISGTWNINTYPSLKPAK
                     QDSGLMTSYFPDRRYLLEASSPTSAGNLDFMLKMLMHQEIDNAKSSGGSIYDNLEEFL
                     THTDATHHGLIFFPFLYGSNTSQDASACFFGLTTKSTKSQMIRAVYEGIAFAHKQHIT
                     DLIKSRGGVPKIIRFSGGATNSPAWMQMFSDILNLPIKTVEGTELGGLGGAILARHAL
                     DKISLKEAVQDMVRVKAIYKPQLSEVKGYEKKYHAYQKLLETLDPIWSELGHLNK"
     gene            13913..14674
                     /locus_tag="VBC94_000838"
     CDS             13913..14674
                     /locus_tag="VBC94_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP endonuclease"
                     /protein_id="HEO6979120.1"
                     /translation="MKQEDIIINSIVFKKQLDKGFTQVQLIKLVKDLGFSRFEIRQEL
                     LQDPDRELPALKAEADFYDINLYYSANEDLIKGGKVNPYLNKGLKEASQLGAPFIKLN
                     VGQTRNLSKEELEPLKEILKSQTIGIKVENNQDPKAATVENCQYFMTLVKELQIPISF
                     VFDTANWAFINQDLYQAVNNLACDTTYLHCKNFIQVAGKPHLSKSLFEGEINLTDLLK
                     SFSNCEYLALEYPTELEILKRDVQRLISISNSQNF"
     gene            14740..16419
                     /locus_tag="VBC94_000839"
     CDS             14740..16419
                     /locus_tag="VBC94_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEO6979121.1"
                     /translation="MIILDKKSYDLLFYLLKLEEPETVMAIANALNQSRRKVYYHLEK
                     INDALPSDVPQIVSYPRVGILLTEKQKAACRLLLDEVTDYSYVMKSSERLQLSLVSIV
                     VAKDRITIDRLMQLNDVSRNTILNDLNELRSELAEKEYNLQLQSTKCRGYFLDGHPLS
                     IIQYLYKLLDDIYHNGSSSFIDLFNHKLSQAFGASTYFSKEVLDYFHHYLFISQRSLG
                     KKINSQDGQFMIQILPFILMAYRKMRLSPEVQTSLNSDFSLVWQRKEYEIAKELADEL
                     EENFQLSLDEIEVGLVAMLMLSFRKDRDNHLESQDYDDMRATLTSFLKELEERYHLHF
                     VHKKDLLRQLLTHCKALLYRKRYGIFSVNPLTEHIKDKYEELFAITSSSVKLLEKAWQ
                     IKLTDDDVAYLTIHLGGELRNSQQSPNKLKLVIVSDEGIAIQKLLLKQCQRYLTNSDI
                     EAVFTTEQYQSVSDLMHVDMVVSTSDALESRFPMLVVHPVLTDDDIIRLIRFSKKGNC
                     ANSNQFTNELEKTIAQYVKEDSERYVLKSKIEKLIHQELLQDVLPLQSTVC"
     gene            16736..17827
                     /gene="ulaG"
                     /locus_tag="VBC94_000840"
     CDS             16736..17827
                     /gene="ulaG"
                     /locus_tag="VBC94_000840"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEO6979122.1"
                     /translation="MPNVKDITRESWILSTFPEWGTWLNEEIEEEVVAEGNFAMWWLG
                     NCGVWIKTPGGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVI
                     DPFAINELDYYLVSHFHSDHIDINTAAAIINNPNLDHVKFVGPYECGEIWKKWGVPEE
                     RIIVIKPGESFEFKDIKVTAVESFDRTCLVTLPVDGAEEHDGELAGLAVTDEEMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDYKIDVAINNYGDNPVGIQDKMTSIDLLR
                     MAENLRAKVIIPVHYDIWSNFMASTDEILQLWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DKDRIEYHHPRGFDDCFEQESNIQFKALL"
     gene            17917..20295
                     /locus_tag="VBC94_000841"
     CDS             17917..20295
                     /locus_tag="VBC94_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEO6979123.1"
                     /translation="MSEFDTKSYLEKLDAWWRAANYISAAQMYLKDNPLLRRELVEND
                     LKVHPIGHWGTVPGQNFIYAHLNRAINKYDLDMFYIEGPGHGGQVMVSNSYLDGSYTE
                     LNPNIEQTEDGFKQLCKIFSFPGGIASHAAPETPGSIHEGGELGYALSHATGAILDNP
                     DVIAATVIGDGEGETGPLMAGWLSNTFINPVNDGAVLPIFYLNGGKIHNPTIFERKTD
                     EELSQFFEGLGWKPIFADVVELSEDHAAAHALFAEKLDQAIQEIKTIQSEARQKPAEE
                     AIQAKFPVLVARIPKGWTGPKAWEGTPIEGGFRAHQVPIPVDAHHMEHVDSLLSWLQS
                     YRPEELFDENGKIVDEIAAISPKGDRRMSMNPITNAGIVKAMDTADWKKFALDINVPG
                     QIMAQDMIEFGKYAADLVDANPDNFRIFGPDETKSNRLQEVFTRTSRQWLGRRKPDYD
                     EALSPAGRVIDSQLSEHQAEGFLEGYVLTGRHGFFASYESFLRVVDSMVTQHFKWLRK
                     SKTHTTWRKNYPALNLIAASTVFQQDHNGYTHQDPGILTHLAEKTPEYIREYLPADTN
                     SLLAVMDKAFKAEDKINLIVTSKHPRPQFYSIAEAEELVAEGYKVIDWASNVSLNQEP
                     DVVFAAAGTEPNLEALAAISILHKAFPELKIRFVNVLDILKLRHPSQDARGLSDEEFN
                     KVFTTDKPVIFAFHGYEDMIRDIFFSRHNHNLHTHGYRENGDITTPFDMRVMSELDRF
                     HLAQDAALASLGNKAQAFSDEMNQMVAYHKDYIREHGDDIPEVQNWKWENIK"
     gene            20672..21895
                     /locus_tag="VBC94_000842"
     CDS             20672..21895
                     /locus_tag="VBC94_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO6979124.1"
                     /translation="MTNILEVKNLTKIFGKKQKAALEMVKQGKSKTEILEKTGATVGV
                     YDASFEIKEGEIFVIMGLSGSGKSTLVRMLNRLIDPSSGNIYLDGKDIAKMNVEDLRN
                     IRRHDINMVFQNFGLFPHRTILENTEFGLEMRGVSKEERTTLAEKALDNAGLLPFKDQ
                     YPSQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDTNKQTI
                     IFISHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTVLEIDGPQVALTRMHREEVSMLMATNRRRQLLGSLTADAAIEARKKGL
                     PLSEVIDKDVVTVSKDTVITDIMPLIYDSSAPIAVTDDNDRLLGVIIRGRVIEALANV
                     QDETVVESPKETVEA"
     gene            21914..23641
                     /locus_tag="VBC94_000843"
     CDS             21914..23641
                     /locus_tag="VBC94_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEO6979125.1"
                     /translation="MENLLQHKLPVAPFVESTTNWITKTFSGLFDFIQTIGNALMDWM
                     TKTLLFINPLLFIVLITIAVFFLAKKKWQLPTFTFIGLLFIYNQGLWEQLINTFNLVL
                     VASLISIIIGVPLGIWMAKSDKVKQVVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVVFALPPTVRFTNLAIREIPLELIEASDSFGSTAKQKLFKVELPLAKNTIMAGIN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGTGFVSGLSLVILAIVLDRVSQFF
                     NSKPGEKQAKTSKVKKWVGLGALALFILAALGRIVVNMTSGNEAKGQKVKIAYVQWDS
                     EVASTNVIAEVLKSKGYDVELTPLDNAVMWQTVANGNADFTTSAWLPKTHGQYFNKYK
                     NSLDDLGPHVENVKIGLVVPKYMNVNSIEELSNQADKQITGIEPGAGIMKSAKQSLKD
                     YPNLSSWKLLSASTGAMTTTLGKAIKNKDQVVITGWSPHWMFAKYDLKYLKDPKKSFG
                     GEEHINTIARKNLKKDMPKVYKIIDKFKWTKEDMESIMLDMDKGMEPAKAAQKWIKNH
                     KKEVSEWTK"
BASE COUNT         7514 a         3899 c         4779 g         7587 t
ORIGIN      
        1 attataaaat caatcgtaaa gataatgtct ataagaagta gtagcgctaa cagataatgt
       61 agtaagaaga ttgttcttaa ccgtttatta atcaatattt catttcctcc tacttattta
      121 ccaataagct tattatatca taatgtataa tcaaacattc gttagtactg attaaaaggt
      181 ttatggaggg gcggttacta ttgtttcatt gaattagtaa ctttaacgac gaaatgttat
      241 aatgttatta gtttataaaa gaggagattg tatgcgtaaa tttttccaag atgttattgt
      301 agaggtggct tattatttag agattgcttt gtcagttatt ctggcaacag ctattatttt
      361 ctttagttta gcactctttc atgatttgtt tagcatgttg tctgttgatc aaaatgttga
      421 taagttgttc cagactttcc ttgctcgcgc catgacaata gcagtcggga ttgagttgat
      481 caaaatgttt tcaaaacctt cccctgatac tttaattgaa gtgttattgt ttgccttagc
      541 tcgtcaactt gtaattgaac atggtagtgt ttcagattac cttatgggga ttgcttcagt
      601 tgctattttg tttgctgttc gtaaatatct tttcacccat tttgatgagt caagcaacat
      661 tgttttacgt gctaataaca aagtcaaaaa agctaatatt ttagctaggg ttcaactacc
      721 ttgtcaaccc aatgaaactt taggagactt acttgttcgt catttaaatg atgaggaaaa
      781 ggacgttgct attggttcag tagtctatat taataacgtt gcattgcgtg ttgatagtat
      841 gcacgatggt cttgtgacgc gtgtagaaat cattaggagt ctacattaaa aaatataaaa
      901 aatcatgtat cttaggatac atgatttttt tgtaaattag gttcagcaca attttgcaca
      961 cttaagtgtg tacttcttgg tcttttttaa aacgcttaca aaaaatataa taaagataca
     1021 aaaaaacatt acttaaataa agagaggtct ttagttatgg aaaacttcct agctcccctt
     1081 aattggttct cgcaaaatat cttgcagaat ccagccttct tcgtaggtct gttggtactc
     1141 attgggtatc ttttattgaa gaaaccactt catgatgtct ttgctggttt tatcaaagcg
     1201 accgttggat atttgatttt gaacgtaggt gcaggaggat tggttaatac attccgtcca
     1261 atcttagttg ctcttgctaa aaaatttcat ttagaagctg ctgttattga cccttacttt
     1321 ggtttagcat cagcaaatgc taagttagaa acaatgggct ttatcagtgt tgcaacaaca
     1381 gcactcttga ttggttttgg gattaacatt ctcttggttg ctcttcgtaa agtaactaaa
     1441 gtccgtactc tttttattac tggtcatatc atggtacaac aagctgcaac aatttcagta
     1501 tttgtactgc tcttgattcc acaacttcgt aatggttttg gtgcatgggc tgttggtatc
     1561 atttgtggtc tatattgggc ggtaagttct aatatgaccg ttgaagcaac tcaacgcctt
     1621 acaggtggtg gcggatttgc catcggacat caacaacaat ttgcaatttg gtttgttgat
     1681 aaagttgcgc catttttcgg taaaaaagaa gaaaatcttg ataacttaaa attaccaaca
     1741 ttcttaaata ttttccatga tacagttgtt gcatcagcaa cattgatgct agttttcttt
     1801 ggaggaatct tagctgtcct tggtccagac attatgtcta atgttaaatt aatcggacct
     1861 ggtgcatttg taccaactaa gcaagctttc ttcatgtaca tccttcaaac atcattgaca
     1921 ttctcagttt accttttcat tttgatgcaa ggtgtacgca tgttcgttac agaattgaca
     1981 aatgccttcc aaggtatctc aaacaaactt cttccaggat cattcccagc ggttgacgtt
     2041 gcagcttcat atggttttgg ttcttcaaat gcagttcttt caggatttgc ttttggtttg
     2101 attggtcaat taattacaat cgctctactt gttgtcttca aaaatccaat cttgattatc
     2161 acaggattcg ttccagtatt ctttgataat gcggcaatcg ctgtttatgc agataaacgt
     2221 ggtggctgga aagcagcagt agctctatca tttatctcag gtattattca ggtggcactt
     2281 ggtgctgttg ctgtaggtct tttaggactt gcaggtggtt atcatggaaa tatcgacttt
     2341 gaattcccat ggttagcatt tggttacatc ttcaagtatt taggtattgc tggttacgtg
     2401 attgtatgtc tcttcttctt agctattcca caacttcaat ttatgaaatc taaagataaa
     2461 gaagcatact atcgtggtga tgcgcctgca gaataataga aatttataac ggaggaaata
     2521 aaaatggtta aagttcttac agcatgcgga aacggcatgg gatcatcaat ggttatcaaa
     2581 atgaaagttg aaaatgcgct tcgtcaatta ggcgttagca actttgagtc agcatcttgc
     2641 tcagtcggag aagcaaaagg tttagctgcg aactatgata tcgtggttgc ttcaaaccac
     2701 cttatccatg aattagacgg acgtacaaaa ggtcatttag taggtcttga caaccttatg
     2761 gatgacaatg aaataaaaac aaaactacaa gaaattttgt aagttaatag acaaagggag
     2821 tgagacaaaa gtttctctcc catatcttta gacttttaag gagaaatcat gaatttaaaa
     2881 caagccttta tcgaaaatga ctcaattcgt cttaaattat ctgcaagcga ttggaaagaa
     2941 gcaatcaaat tatcaattga tccattaatt gaaagtggtg ctgttgatgc agaatattac
     3001 gatgccatta ttgaatctac agaggaattt ggtccgtact acattcttat gccgggtatg
     3061 gcgatgcctc atgctcgtcc agaagcaggc gtaaaacgtg atgcattttc attaattaca
     3121 ttaacagagc cagtagtctt tccagatggc aaagaagtct cagtattgtt agcacttgct
     3181 gcaacaagtt cagctattca tacttctgtt gctatccctc aaattattgc cctttttgaa
     3241 ttggaaaatt caattcagcg cttgacggaa tgccaagaag caaaagaagt ccttgcaatg
     3301 gtagaggaat caaaaaacag cccatattta gaaggtttag atttagaaag ctaatcaata
     3361 aactgatatt aatagaaagg ataggactta taaaaaatct ctgtgataag aaactctttg
     3421 ccacaaataa agtgagtatt tttatcaggt ccttagaata aacaacatga caaaacgttt
     3481 accaaattta caagttgcac ttgaccactc agatttacaa ggagctatca aagctgccgt
     3541 atcagtagga catgaagttg atgttattga agctggaact gtttgccttc ttcaagtagg
     3601 tagtgaatta gttgaggtcc ttcgcagtct cttccctgac aaaataattg ttgctgatac
     3661 aaaatgtgct gatgctggtg ggactgttgc taaaaataat gctgttcgcg gtgcagactg
     3721 gatgacttgt atctgttgtg ctactattcc aacaatggaa gcagccttaa aagctatcaa
     3781 agaagaacgc ggcgatcgtg gagaaattca aatcgaacta tatggtgatt ggacttatga
     3841 gcaagcgcaa caatggttgg atgcaggtat ttcacaagcc atttatcacc aatctcgtga
     3901 tgcgcttctt gctggcgaaa catggggaga aaaagatctt aataaggtta aaaaattgat
     3961 tgatatgggc ttccgagttt ctgtaactgg tggtttatca actgatactc ttcaattatt
     4021 tgaaggtgtt gatgtcttta cttttattgc gggtcgtggt attacagagg cagatgatcc
     4081 agccgcagca gcacgtgcat ttaaggatga aattaaacgt atttgggggt aatgtatgac
     4141 acgtccaata ggaatttacg agaaggcaac accaaaacat tttaactggt tagaacgcct
     4201 acaatttgct aaagaattgg gatttgattt tgtagaatta tccattgatg agtcagatga
     4261 acgtttggca cgtttggagt ggtcaaaaga agaacgcctt gagttggtca aagctatttt
     4321 tgagacagga gttcgtgttc caacaattac atttagtggt catcgtcgtt tcccaatggg
     4381 atctaatgat cctgagaaag aagcgcgtgc gatggatatg atgaaaaaat gtatcgtctt
     4441 tgctcaagat attggtattc gtaatattca attagcagga tatgatgtgt actatgaaga
     4501 aaaatctcca gagacacgtg cacgctttat taagaatctt cgtcaagctt gtacttgggc
     4561 cgaagaagca caagttattc tgtctattga gattatggat gacccattta tgaattccat
     4621 tgagaaatat ttggctgtgg aaaaagagat tgattctcct tatctctttg tctacccaga
     4681 tactggaaat gtatcagcat ggcataatga tctttggagt gaattttata atggacatcg
     4741 ctcaattgca gcgcttcata ttaaagatac ctatgctgtt accgaaacgt ctaaaggtca
     4801 attccgtgat gttccatttg gtcaagggtg tgttgattgg gaagaaatgt ttgctgtcat
     4861 taagaaaaca aattataatg gtccattctt gattgaaatg tggtcggaga actgtgagac
     4921 tgtagaggaa acacgtgcag ccattaaaga ggcgcaagat tttctttacc cattaatgga
     4981 gaaagcagga gtgaaataat atggcaaaat cattgcaaga aatgcgtgaa cgcgtgtgtg
     5041 aagctaataa atcactacca gttcatggtt tagttaaatt tacttggggt aacgtttctg
     5101 aagttgaccg cgaagcagga ttgattgtta tcaaaccttc aggagttgac tatgaccaat
     5161 tgacacctga aaatatggtt gtcacagact tagagggaaa tatagtggaa ggggatttga
     5221 atccatcatc tgatcttcca actcatgttc aactttataa agcatggcca gaagtcggtg
     5281 gtattgttca tactcattca acagaagcag ttggctgggc acaagcgggt cgcgatattc
     5341 cattttatgg aacaacacat gcagattatt tttatgggcc agtaccatgt gctaggtcac
     5401 ttagtgaaga tgaagttaac actgcttatg aaaaagaaac aggtagtgtt attatagagg
     5461 aatttgaacg tcggaattta gatccaatgg ctgttcctgg tattgttgta cgtaatcatg
     5521 gaccatttac ttgggggaaa gatccagcac aagcagttta tcactcagtc gttcttgaag
     5581 aagttgccaa gatgaatcgt ttcactgaac aaattaatcc acgtgttgaa ccagctccaa
     5641 aatatattat ggataaacac tatttacgta agcatggacc aaatgcttat tatggtcaaa
     5701 aaggacaaga acattaagga gaataatatg aaatactttt tagatactgc cgatgtttct
     5761 gaaattcgtc gtcttaatcg tttaggaatc gttgatggtg ttactaccaa tcctactatt
     5821 atttcacgtg aggggcgtga ctttaaagaa gttattaacg aaatttgcca aatcgttgat
     5881 ggtccagtta gtgcagaagt gacaggttta acttgtgatg aaatggttac cgaagctaga
     5941 gaaatcgcta agtggtcacc aaatgttgtt gttaaaattc caatgactga agaaggttta
     6001 gcagcagttt cacaattatc aaaagaaggt ataaagacaa atgtgacatt aatatttact
     6061 gtggctcaag ggctttcggc aatgaaagct ggtgcaacct ttattagtcc ttttgtgggt
     6121 cgtcttgaag atattggaac agatgcttat gctttgattc gtgaccttcg tcacattatt
     6181 gatttttatg gtttccaatc tgagattatt gctgctagca ttcgtggatt ggcgcatgtg
     6241 gagggcgtcg ctaaatgcgg tgcgcacatt gccactattc cagataaaac atttgcatca
     6301 ctatttactc acccgcttac tgataaaggg attgaaactt tcttaaaaga ttgggatagt
     6361 ttcaagaaga agtagtgtta cgataaagca aactcttaat tatactagtg aaagacaaac
     6421 tttgataatg ggtttgtctt ttttataatc ttctccaata agaacactta ttttcatttt
     6481 tcttattaaa tgatataata cggataaatt aagtctaatt agaataagga aatagagatg
     6541 tttgagaagg taaccattag tgatattgca aatttagtag gtgtgtcaaa agctacagtt
     6601 tcctattatc tcaatgggaa ctataagaaa atgtctctgc aaacgaaaga aaaaatccgt
     6661 ttagctatta aggagactgg ttatcaacca agtaagattg ctcaaagttt agttacaaaa
     6721 aatacccgta ctattggggt cgttattgct gatattacca atccatttat ttcgtctgtg
     6781 atgaagggaa tacatgatac ttgtcaacaa tttggttact cggttaactt tactaactcg
     6841 gataatgata ttgacattga gttggaaaat ttaaaccgtt taaatcagca aaacgtttca
     6901 ggaattattt tagattctgt agatcctaat cattcattta tagaaacttt gagtaatgat
     6961 cgtcttgtca tggttgatag acaagctaaa gatattaagg tagatactgt agtatccgac
     7021 aataaagaat cgactcaaat atttcttgaa aaaatgcaag aaactgggta tcatgacatc
     7081 tattttgtca cttatcccat tgaagggatt tcaacccgtg agcttcgtta cgaaggtttt
     7141 aaagaagtgg tcagttcaaa tcctgataag ttgattatta tcactgaaga tggtagcaca
     7201 cagagaattc ttgatattat tgaacattca gagcaaaaac cagggttctt aatgatgaac
     7261 ggtccaactc ttttgaattt tatgaaaaaa ttaaatcaat caacggtcag ctaccctgag
     7321 gattatggtc ttgggagtta cgaagattta gaatggatgc aggtgcttac accaaacgtt
     7381 tcctgtataa aacaggattc atatggcatt ggttgtctgg ctgcccaatg tttaattgaa
     7441 aaaattagtc aaggaaacga accaactact gcaaggttac tagaagtaaa aaatcaaatc
     7501 gttattcgtc aatcttttta aaaatgtttg cgagagcaag cattttttta taaaaaagca
     7561 aaaaacacta tgtaaacgct tgcttttata aaaaatgata gtataataga actataaatg
     7621 agttaaccgt ttaacttaag aataggtaag cttttaacga accatctcta actctcttaa
     7681 ctcaaatgtt tttatttttt tgcaccatta gttaaacggt taactcaagt tttatcttca
     7741 ccaaaggagg atagaatgaa gattaataaa ttattgtcag acagacttga agcttgttat
     7801 tcagtttgtc gtttgcaaaa tcaagactat gattttttat tagtagcttc agaggttgat
     7861 caagcctgct ttgcttatga tttgaatagc gatttagcaa aaattgttgt ttgggaagat
     7921 gtgggaggta ccatgagtat tattccaatt cccggaacca tggatttttt ggcaacccag
     7981 aaattttacc cagggtttaa ggccaaagat tgtcaggttg tttatgcaag ttttcaagga
     8041 gatcattggg acatttgtaa cgcatttgat tttccgtatt tacatcgctt tgatctcatt
     8101 aaaggtaaag aaaatcaact ttactttata ggttgtacaa ttgctaacag taaagcctac
     8161 actgaggatt ggagtgataa aggccgaatt tttgttgctc gttttaatac tcaaaaccat
     8221 acattggaag gattgcaaca attgcctcaa actttattaa aaaatcatgg atactatgcc
     8281 attcaggatg aaggatattc attgattact tcagtagaag gggtactcaa actcacttat
     8341 ccagaatttt ctactacagg cgactggcaa ttagaacggc ttttcgatga ggagacaagc
     8401 gatgtggtga aagtggatat taatcgggat ggtaaggatg agtatgtgat catccaaggt
     8461 tttcatggag atcgtttacg tatcttcact gaagatttcg gtcgagaatt attccattat
     8521 cctgaaaaaa ccccatttgg tcacgctatt tggagtggtc gtttacttaa tcagacttgt
     8581 ttcgtattcg ggtggcgatc agaaaaagca gaattaaggc tttttcactt tgtagatggg
     8641 cacttggttt cagaattagt agatgcaaaa gcagcttcta gtaatgtctt agcttttgaa
     8701 aaagatggaa aagcttatct tttctcagcc aataacggac gtggcgaagt tgctctttat
     8761 caattagtaa aataaattta agaaggagac aatatggaca agaaaaaaat cttagtaacg
     8821 ggtattgtgc ctaaagaagg tctaagaaag cttatggacc gatttgatgt tacttattca
     8881 gaagatcgcc cattttcacg tgactatgtg ttagagcatt tatctgaata tgacggatgg
     8941 ttactcatgg gacaaaaagg tgataaagag atgattgatg caggtgaaaa cttacaaatt
     9001 atttctttaa atgcggttgg ttttgatcat gtagatactg cgtatgctaa agaaaaagga
     9061 attgtagtct ctaattcccc tcaagcagta cgagttccga cagcggaaat gacttttgct
     9121 ttaattttag ctgccagtaa gcgccttgca ttttatgata gtattgtaag aagtggagag
     9181 tggattgatc cgagcgaaca acgttatcaa ggtttgactt tgcaaggttc aacattaggt
     9241 atttacggaa tgggacgcat cggtttgaca gtcgcaaatt ttgctaaagc atttgggatg
     9301 actgttgttt acaatgatgt ttatcgactt cctgaagata aggaaaaaga gcttggtgta
     9361 acctatctcg aatttgatca gcttattgaa acagctgatg tcataaccat ccacgcccca
     9421 gcattaccat caacaatcca caaattcaat aaagatgttt ttgctaagat gaaaaatcga
     9481 agctacttaa tcaacgctgc tagaggccca atcgtttctg aagaagcact tattgaagct
     9541 ctgaaagaag gtgaaatcgc gggtgcggga ttagatgttt ttgagaatga accacaggta
     9601 tctgaaggct tgagaagtct tgataatgtt atcatgagtc cacatgctgg gacaggtact
     9661 attgagggac gtagaacttt ggctgaagaa gcagctgaca acatcattgc cttctttgat
     9721 ggcaagccac aaaatattgt caataagtaa ttggctagtg agtgcagagc catcttttga
     9781 aaaaatggga cctcccattt ttttacgggt aaatgtaagc gcttaataat ttaataaagg
     9841 tgaacttatg ctagaagctt tacataaatt ctttgaagtt tttggtgcag caactgttgt
     9901 acccgctatg atttttattg tcagcctttt tctaaaggtc aatccaaggc gagctttctt
     9961 tagcgcctta cgagctggtg ttggactcac tggctttggt tggattatat cagcttttgc
    10021 tcctatggtg actaagtata tccagcagat ggttaaaact actggtctcc atttaccaat
    10081 tgtagatatt ggttggcagg ctggttctct caccgccttt tcttctgaga ttggcctctc
    10141 tttctttgtt tttggcttgc taattgaatt ggggcttttc ttgcttggga ttacccgagt
    10201 atttgtccca tctaatcttt ggaataactt tggctatatg atttggggta caatggccta
    10261 tgcagcaaca ggtaatttta tattgtcttt cgcatttatg gtttttgtct tactctattc
    10321 attagtgatg tcagaagtat tggcagatcg atggtcggag tattacggtg ttaaaaatgc
    10381 aacgatcaac tcgattcata atattgaaac ccttattcca gctcttattc ttgatcctct
    10441 ttggaaccta cttggtgtca ataaggtaaa gttgaatcca gaatcattaa agactaagtt
    10501 agggatattt ggtgagccaa tgacacttgg ttttatactt ggtgtgatta ttggtgtatt
    10561 aggtagttta cgtaaccttg caagtattga tacttgggga ggtatacttg gttttgcagt
    10621 tgctcttgca gcggttatga ctatttttcc tttgattacg ggggtatttg ctagtgcttt
    10681 tgctcctctt gctgaagcag ttgaacgcaa taagaaaaaa gaaagtcaag ctgagcaagg
    10741 agcactggat aaaaaacgtt ggtttattgc agtagatgac ggtgttggtt ttggagaacc
    10801 agcaactatt atcgcaggtc tcattttagt accaataatg gttgttattt ccttgatttt
    10861 acctggaaat gaagctttac cagtcgttga ccttattgct attcctttta tgattgaagc
    10921 tatgatagct gtgtcaaaag gaaatatcct taaagctatt ttgaatggaa tcatatggtt
    10981 ttcactaggt ctttatgctg ctagtgccct aggtccaatc tacacagaag ctgttaagca
    11041 ttttggaact gctttaccag caggggtaac tttgattatg agctttaatc tcctagctca
    11101 tcctttgaca gctctagtct tctttgcttg gatatctggg aatccactct atattggtgt
    11161 gacagttatt atctatcttg ctttcttgtt cttgcttcgt actcgtcgtg aatctattta
    11221 cgcatacctt aaaactatgg ctgataagaa tagcggatat gaaggtagtt gtgaaaaaat
    11281 ctttattgct gaagattaaa aatctgttgg aggaatactc atgaaaaaaa ttgatggaca
    11341 tgctcatatt gttgatacaa ttgcaggatt taatggtaaa gggcgtctta atgcccttgg
    11401 aaacggttat gctatttggg atgatggtga tttaattaag ctcatcccag acgaatacgg
    11461 tgacaaagca tttacagctg aatctttttt gaagtatatg gatgctaatg gtgtagataa
    11521 agcagtcatt ttgcaaggtc atcttaatgg ttatcaaaat tattatacgc atttagctat
    11581 taaacgttat ccagaacgtt ttacaggcgc attctctgtt gatccatttg cagataatgc
    11641 aatgcaaatt gtaaaacgtc atgttgaagt ccttggtttt cgagctatta aatttgaaat
    11701 tagtcaagga gggggtatcc atggataccg tggacaaaaa acgcctttcc gtcttgatac
    11761 agacccacat gtgagtcgta ttttgactta tctgttagac tatccaggat ttgtagttac
    11821 agttgattat ggcaattggg atcaaattag tcaccaacct gatgctattg ctaatttagc
    11881 tcgcctttat acgagtctcg attttgttgt ttgtcacctc tcttttccac atgttgagca
    11941 ttcaaatcga ttgcgtgctg aacttaatat gtggaaaaac ttcaacaata tctatactga
    12001 tatttcagct attcaagata ttgatgcccc agatagtttt ccttttccaa aatcagaaca
    12061 aaatgtacgt attgctaaag aagttcttgg ggctaagcgt atcatttggg gaactgattc
    12121 tccatggtca gcgaccttca atacttacga agaattagct acttggctag agaatgttga
    12181 tattttcagt caagaggaac tagaagatgt tatgtacaat aatgctgagc gtgtttactt
    12241 taaagaagaa catgtaaagg ctaacagagc aggtatagat gatacaacca aagagctcaa
    12301 tctttattag aagagttttc aaggtacaag agtttcttgt acctcctttt taaatcaaca
    12361 atgaggagac aaattatgac ttactacctt agcattgact acggcggtac aaataccaag
    12421 gcgcttattt ttgacaaatt aggacaccaa atcgctgttt cgagttttga aactttaaaa
    12481 aatgaggctc aatctggtca tcgtcaagta aaccttgtta aaacgtggaa tgctataact
    12541 tctgctatta gagaggttat tcaaatctca aaactcagcc ctgagcagat tagtgcagta
    12601 gcatgtattg gacatgggaa aggtctttat ctgctagata ataagttgga gccacttgaa
    12661 caaggaattt tgtctacaga taatcgtgcc aaagatttgg cgcaatattt cgaatctaaa
    12721 cttgataata tttgggagtt gactcggcaa catattttcc cttcacaaag tccagttatt
    12781 ttacgttggc ttaaagatta tcagcccgaa acctataaat caataggggc ggtcctttct
    12841 gcaaaggact ttattcggta taagcttaca ggaaaagtac agcaagaata tggtgacgct
    12901 tcaggtaatc attggataaa ttttcaaaca ggaacttatg atccagctat tttagatttt
    12961 tttggcatta gagagataga aaactcactt cctgaactta tagatagtgc agatttagtt
    13021 cctgggggaa ttagttctca agcagcaaaa gagactggtc ttgtagaagg aacccccgtt
    13081 gttggagggc tctttgacat cgatgcttgt gctcttggat caggtgtttt agagtcagat
    13141 acttttagtg ttatttcggg aacttggaat attaatactt atccaagttt aaaaccagca
    13201 aagcaagata gtggtcttat gacttcctat tttccagatc gtcgttatct cttagaggca
    13261 agtagcccta cttctgcagg gaatcttgat tttatgttaa aaatgctcat gcatcaagaa
    13321 attgataacg ctaaatctag tggaggttct atctatgata atttagaaga atttctcact
    13381 catactgatg ccacacatca tggacttatt ttctttccgt ttctttacgg tagtaacaca
    13441 tcacaagatg ctagcgcttg cttttttggg ctaacaacta aatcgacgaa atctcagatg
    13501 atacgtgcgg tatatgaagg tattgcgttt gcacataagc agcatatcac tgatttaata
    13561 aaaagtaggg gcggtgtgcc aaaaataatt cgtttctctg gcggagctac caactcacca
    13621 gcatggatgc aaatgttttc tgatatctta aaccttccta ttaaaacagt agaaggcaca
    13681 gaattaggag gattaggagg agctatttta gcacgtcatg ctttagataa gatttcgtta
    13741 aaggaagcag tccaagatat ggttcgtgta aaagctattt ataaacctca attatccgaa
    13801 gtaaaggggt acgaaaaaaa atatcacgcc taccaaaaat tattggaaac actggatcct
    13861 atttggtcgg aactcggtca tctgaataag tagggatatc aaaggagttg ttatgaaaca
    13921 agaagatatt attatcaata gtattgtatt caaaaaacag ttagacaaag gttttacaca
    13981 agttcagctc attaagttag taaaggattt aggattttca cgatttgaga tacgtcaaga
    14041 attgcttcaa gatccagata gagagctacc cgctttaaaa gcagaggctg acttttatga
    14101 tatcaatctt tactatagtg ctaatgaaga ccttatcaaa ggagggaaag ttaatcctta
    14161 tcttaataaa ggccttaaag aagcatcaca gttaggagct cctttcatta agctaaatgt
    14221 tggacaaacc agaaacttaa gtaaagaaga gttagaacct cttaaagaga tattgaaaag
    14281 ccaaactatt ggtataaagg tggaaaataa tcaagaccct aaagcagcta cagtggagaa
    14341 ttgccaatat tttatgactc tagtgaaaga attacaaata ccaataagtt ttgtttttga
    14401 tactgctaat tgggccttta tcaaccaaga cctataccaa gcggtaaata acttagcttg
    14461 tgataccacc taccttcact gtaaaaattt tatacaggta gctggaaagc cccacctctc
    14521 gaagagcctc ttcgaaggag agattaatct gactgatttg ttaaagagtt tctctaattg
    14581 tgagtatcta gctctagaat accccacaga gttggagata ttaaaaaggg atgttcaacg
    14641 tttaatctct atcagtaata gtcaaaactt ttaatgaaaa ggctttaaaa agatgttata
    14701 ataagtctgt atgaattcga gtagaaaaga ggtatattct tgattattct cgataaaaaa
    14761 agttatgacc tcctctttta cctattgaaa ttagaggaac ctgaaacagt tatggcaatt
    14821 gccaacgcac ttaatcagtc tagacgtaaa gtgtattatc acttagagaa gataaacgat
    14881 gcactgccta gcgatgtgcc tcagattgtt agttatccac gtgtaggaat cttgctaaca
    14941 gaaaaacaaa aagcagcctg tcgtctttta ttagatgaag tgactgatta tagttacgtc
    15001 atgaaaagta gtgagaggtt gcagctgtct ttagtatcta tcgtagtagc taaggaccgc
    15061 ataacgattg ataggttgat gcaactaaac gatgtttctc gaaatactat cttaaacgat
    15121 ttaaacgaat taagaagtga gttagcagag aaagaatata atttacagtt acaatcaaca
    15181 aaatgtcgtg gttatttttt agatggtcat ccattgtcca ttatccagta cttatataag
    15241 ctcttagatg atatctacca taatggaagt agtagtttta tagacctttt taatcataaa
    15301 ctgtctcaag cttttggtgc cagcacttat ttttctaaag aggttcttga ttattttcat
    15361 cattatctct tcatttctca acgaagtcta ggtaagaaaa tcaacagtca agatggtcag
    15421 tttatgattc agattttgcc ttttatacta atggcttatc gtaagatgcg attaagtcct
    15481 gaagtacaga cctctcttaa tagtgatttt agcttggttt ggcaacgtaa ggaatatgag
    15541 attgctaaag agttggctga tgagctggaa gaaaattttc agttatcact ggatgagatt
    15601 gaagtgggac tagtagccat gcttatgctt agtttccgca aggaccgtga caatcattta
    15661 gagagccagg attatgatga tatgcgagct actctaacca gttttttgaa agaattggaa
    15721 gaacgatatc accttcactt tgttcataaa aaggacttac taagacaact tcttactcac
    15781 tgcaaggcac tcttatatcg taaacgttat ggtatttttt ctgttaatcc tttaacagag
    15841 catattaaag acaaatatga agaacttttt gccataacct cgtcttctgt aaagctttta
    15901 gagaaagctt ggcaaatcaa attgaccgat gatgatgtag catatctaac gattcattta
    15961 ggaggggaac ttcgtaatag tcaacaatct cctaataaac ttaagttagt tattgtatct
    16021 gatgaaggaa tagcgattca gaaacttctt ttaaagcaat gtcaacgcta cttaacaaat
    16081 agtgatatag aagctgtttt tacaaccgaa cagtatcaaa gtgtgagtga tcttatgcat
    16141 gtagatatgg ttgtctctac tagtgacgct ttagaatctc gttttccgat gttagtagtt
    16201 caccctgttt tgacagatga tgatattatt cgcttgattc gcttttctaa aaaaggtaac
    16261 tgtgcaaata gtaatcaatt taccaatgaa cttgaaaaaa caattgctca atatgtcaag
    16321 gaagatagtg aacgctacgt gctgaaatct aagattgaga aacttattca tcaagaattg
    16381 ctccaagacg tccttcccct tcaaagtaca gtttgttaaa atataatttt atggtagcct
    16441 ctcagctttt ctgaagacag aaaaagaagc tggggctttt ttggtgcttt taaacaatgt
    16501 gctagatatt taatcgcaat gagttcctct atgacatgat aattaaagtt gagaaagcta
    16561 gtacttttaa tgggagtact ttttatttgt gcttatttta attaaaatta tgagtctaat
    16621 ttggaacaga cagcccagtt ttgaacacat aaatgtgtga cacttggtct ttttttcaaa
    16681 agtatttgaa cttatactag tagatgtaag aaacaattaa tcaggaggaa ataaaatgcc
    16741 taacgttaaa gatattacta gagaatcatg gattttatca acattcccag aatggggtac
    16801 atggctaaac gaagaaattg aagaagaagt tgttgctgag ggcaactttg caatgtggtg
    16861 gttaggaaac tgtggtgttt ggattaaaac accaggtggt gctaatgttg ttatggacct
    16921 atggtcaaac cgcggtaaat caacaaaaaa agtaaaagat atggttcgtg gacaccaaat
    16981 ggctaatatg gcaggtgttc gtaaactcca accaaactta cgcgcacaac caatggttat
    17041 tgatccattt gctattaatg aattggatta ctatcttgtt tcacatttcc acagtgacca
    17101 cattgatatc aacactgcgg cagccattat taataatcct aacttagacc atgttaaatt
    17161 tgttgggcca tatgaatgtg gtgaaatttg gaaaaaatgg ggagttccag aagaacgaat
    17221 catcgtgatt aaaccaggtg aatcatttga gtttaaagat atcaaagtta ctgctgttga
    17281 atcatttgac cgtacttgct tggtgacact tccagtagat ggtgcagaag aacatgatgg
    17341 agaacttgct ggtttagccg tgactgatga agaaatggct cgtaaagcag ttaactatat
    17401 ttttgaaaca ccaggtggaa caatctatca tggagctgac tcacattttt caaattattt
    17461 tgctaaacat ggtaaagact acaagattga cgtagctatt aacaactatg gtgataaccc
    17521 agttggtatt caggataaaa tgacttcaat cgatcttctt cgtatggcag aaaatctccg
    17581 tgccaaagtc attattcctg tacactacga tatctggtct aacttcatgg cttctacaga
    17641 cgaaatctta caattgtgga aaatgcgtaa agaacgcctt caatatgact tccatccatt
    17701 tatttgggaa gttggaggta aatacactta tccacaagat aaagatagaa ttgaatacca
    17761 tcacccacgt ggttttgatg attgcttcga acaagaatca aatattcaat ttaaagcact
    17821 tctataatgg ctaatgagag actgtatgtc gcatgatatg cagtttttca attttaaaat
    17881 atgttactaa aatcacaatt aagattagga gaaataatgt cagaatttga tacaaaatca
    17941 tatttagaaa aacttgatgc atggtggaga gcagctaatt atatttctgc agcacaaatg
    18001 tatctaaagg ataaccctct cttgagacga gagctggttg aaaacgactt aaaggttcat
    18061 ccaattggtc actggggcac tgtacctgga caaaatttta tctatgctca cttaaatcgc
    18121 gctattaata aatacgattt agacatgttc tatattgaag gtccaggtca tggtggtcaa
    18181 gttatggtat ctaattcata tttagatggt tcatatactg aattaaaccc aaatattgag
    18241 caaacagagg atggctttaa gcagttatgt aaaatcttct ctttcccagg tggaattgca
    18301 tcccatgctg caccagaaac accagggtca attcatgaag gcggtgaact tggttacgca
    18361 ctttctcatg cgacaggtgc catcctagac aatccagatg ttattgctgc gactgttatt
    18421 ggtgatggtg aaggtgaaac aggaccactt atggctggtt ggctatctaa tacctttatc
    18481 aatccagtaa atgatggtgc tgttctacca atcttctatt taaatggtgg taaaatccat
    18541 aacccaacta tctttgaacg taagacagat gaagaattat ctcagttttt tgaaggatta
    18601 ggttggaaac ctatttttgc agatgttgtt gaactctctg aggatcatgc ggctgctcat
    18661 gctttgtttg cagaaaaatt agatcaagct attcaagaga ttaaaaccat tcaatcagaa
    18721 gcacgacaaa aaccagcaga agaagctatc caagcaaaat tccctgtctt agttgcacgt
    18781 attcctaagg gttggactgg tccaaaagct tgggaaggaa caccaattga aggtggcttc
    18841 cgcgctcacc aagtaccaat tccagtagat gcccaccata tggaacatgt tgattctctt
    18901 ttgtcatggc ttcaatcata ccgtccagaa gaattatttg atgaaaatgg taaaatcgtt
    18961 gatgagattg ctgctatttc accaaaaggt gatcgtcgca tgtccatgaa tccaataacc
    19021 aatgctggta ttgttaaagc aatggataca gcagattgga agaaatttgc tcttgatatt
    19081 aatgttccag gtcaaattat ggcacaagat atgattgaat ttggaaaata tgcagcagat
    19141 ttggtagatg ctaatccaga taatttccgt atttttggtc cagatgaaac gaaatcaaat
    19201 cgtcttcaag aagtgtttac acgtactagc cgtcaatggc ttggtcgtcg taaaccagat
    19261 tatgacgaag ctttaagtcc agctggacgt gttattgatt cccaattgtc tgaacaccaa
    19321 gcagaaggtt tcttagaagg ttatgtttta actggtcgtc acggcttctt tgcttcatac
    19381 gaatcattcc ttcgcgttgt agattcaatg gtaacacaac atttcaaatg gttacgtaaa
    19441 tcaaaaacac acacaacatg gcgtaaaaat tatccagcgc ttaacctaat tgcagcttca
    19501 actgttttcc aacaagatca taatggttat actcaccaag atccaggtat tttaactcat
    19561 ttagctgaaa aaacacctga atacattcgt gaatatttac cagcagatac taactcactt
    19621 ctagctgtta tggataaagc gtttaaagct gaagataaaa ttaatttaat tgtgacatct
    19681 aagcaccctc gtccacaatt ttactctatt gctgaagcag aagagttagt ggcagaagga
    19741 tataaggtga ttgactgggc ttcaaatgtg tcgcttaatc aagagccaga tgttgtcttt
    19801 gctgcggcgg gaacagaacc taacttagaa gctttggcag ctattagcat tcttcataag
    19861 gctttcccag aacttaagat tagatttgtt aatgtattag acatcctaaa acttcgtcat
    19921 ccttcacaag atgctcgcgg tttatcagat gaagagttta acaaagtctt taccacagat
    19981 aaacctgtta tttttgcttt ccatggttat gaagatatga ttcgagatat tttctttagt
    20041 cgtcacaatc ataatttgca tacgcatggt taccgtgaaa atggtgacat cacaacacca
    20101 tttgatatgc gcgtgatgtc agaattggac cgattccatc tggcacagga tgcagctctt
    20161 gcttctttag gaaataaagc acaagccttc agtgatgaaa tgaatcaaat ggttgcttat
    20221 cacaaagact atattcgtga acacggagac gatattccag aagtacaaaa ttggaaatgg
    20281 gaaaatatca agtagtcact tttttcaggc ctatacttac aaacaaaaac acttttagat
    20341 tttttctaag ggtgttttgt tattttatag aagaatgacc gctttttcaa aagtattaag
    20401 tggtcacttt taaagttgta atattaatac taataagtct ttaaataatt caataatgcc
    20461 ataacaataa tggtgtgggc taattaaaat atctctaagc aaaggtgttg acataaagag
    20521 accatacatg atattatagt tatgttctgt ttcggcagtt tatttttttg agtaaaaaag
    20581 tgactaccct tttacgataa cttaacgtca ctttatagat aagacacata accttaaaaa
    20641 taagatacga aatttttagt aggagataag aatgacaaat attttagaag tgaagaattt
    20701 aaccaaaatt tttgggaaaa agcaaaaagc tgctcttgaa atggttaagc aaggaaaaag
    20761 taagacagaa attcttgaaa aaacaggagc gactgtaggt gtctatgatg cgagttttga
    20821 aataaaagaa ggcgaaattt tcgttatcat gggactttct ggttcaggaa aatcaacttt
    20881 agttcggatg ttaaaccgtt tgattgaccc atcatcagga aatatctatt tagatggtaa
    20941 agatattgca aagatgaatg tagaagattt acgtaacatt cgtcgtcatg atatcaacat
    21001 ggttttccaa aattttggtc ttttcccaca tcgtacaatt ttagaaaata cagagtttgg
    21061 gttagagatg cgtggtgttt ctaaggaaga acgtacaacg ttagctgaaa aagctttgga
    21121 caatgcaggt ctattaccgt ttaaagatca gtaccccagt caattatcag gaggaatgca
    21181 acagcgtgtt ggtttagcac gtgcccttgc taacagccct aaaatccttc tgatggatga
    21241 ggccttctca gctttagacc cattaattcg tcgtgaaatg caagatgagc ttcttgatct
    21301 tcaagacacg aataaacaaa ctattatttt tatcagccat gatttgaatg aagcgttacg
    21361 tattggtgat cgtattgctc tgatgaaaga tggagaaatc atgcagattg gtacaggaga
    21421 agagattttg actaatccgg ctaatgattt tgttcgtgag tttgttgaag acgttgatcg
    21481 ctcaaaagtg ttaactgctc aaaatattat gataaaacct ttaacaactg ttttagaaat
    21541 tgatggccca caagttgctt taacaagaat gcatcgtgag gaagtctcaa tgttaatggc
    21601 aacgaatcgt cgtcgccaac ttctgggatc cctaactgca gatgctgcaa ttgaagctcg
    21661 taagaaaggt ttgccgcttt ctgaagtgat tgataaagat gttgtaacgg tctcaaaaga
    21721 tacagttatt acagatatca tgcctttaat ttatgattca tctgctccga tagccgttac
    21781 tgatgataat gatagactcc taggggttat cattagagga cgtgtgatcg aagcacttgc
    21841 taacgttcaa gatgaaacag tagtagaatc gccaaaagaa acggtagaag cttaatagag
    21901 aggacaattc attttggaaa atttattaca acataagtta ccggtcgcac catttgtaga
    21961 gtcaacgacc aattggatta cgaaaacttt ttctggttta tttgacttta tccagacaat
    22021 cggtaatgca ttgatggatt ggatgacaaa aacattacta ttcattaatc ctttactttt
    22081 tattgtacta attacaattg ctgtattttt cttagctaag aaaaaatggc aattaccgac
    22141 atttactttc attggtttgc tatttatcta taaccaaggg ctgtgggaac agttgattaa
    22201 tacttttaac ttggttttag tagcaagttt aatttctatt attattggtg taccactagg
    22261 tatttggatg gcaaaaagtg ataaagttaa acaagttgtt aatccaattc ttgactttat
    22321 gcaaactatg ccggcattcg tttaccttat tcctgcagta gccttctttg gtatcggtat
    22381 ggtaccaggt gtttttgcct ctgttgtatt tgctctgcct ccaacagtgc gctttactaa
    22441 cttggctatt cgtgaaatcc cacttgaatt aatagaagca tctgattcat ttggttcaac
    22501 agctaagcaa aaattattca aagttgaact accacttgct aagaacacta ttatggcagg
    22561 aatcaatcaa actatgatgt tggctctttc tatggtggtt acaggatcta tgatcggtgc
    22621 acctggactt ggtcgtgagg tgttatcggc gcttcaacat gctgatattg gtacaggatt
    22681 tgttagtggt ctatcgctag ttattttagc aatcgtgtta gaccgtgtta gtcaattctt
    22741 taattccaaa ccgggtgaga agcaagctaa aacatcaaaa gttaaaaaat gggtaggttt
    22801 aggggccctg gctctcttca ttctagcagc tcttggtcgc attgttgtca atatgacttc
    22861 aggaaatgaa gccaaaggtc aaaaagtgaa gatagcttat gtccaatggg attcagaagt
    22921 tgcttcaacc aacgttattg ctgaagtttt aaaatctaaa ggatatgatg ttgaattgac
    22981 accacttgac aatgcggtca tgtggcaaac agtcgccaat gggaatgctg attttacaac
    23041 aagtgcttgg ttaccaaaga cccacgggca atattttaat aaatacaaaa atagtttaga
    23101 tgacttaggt cctcacgttg agaacgtcaa aataggttta gttgttccta aatacatgaa
    23161 cgtaaatagt attgaagaat tatcaaatca agcagataaa caaattacag gtatcgaacc
    23221 aggtgcaggt attatgaagt ctgctaaaca atcccttaaa gattatccaa acctttcaag
    23281 ttggaagttg ttatcagcat caacaggagc tatgacaaca accttaggga aggctatcaa
    23341 aaacaaagat caagtagtca ttacaggttg gtcacctcac tggatgtttg ctaagtatga
    23401 tttaaaatac cttaaagatc ctaagaaatc atttggtggt gaagaacata tcaatacaat
    23461 agcacgcaag aatttgaaaa aagatatgcc aaaagtatat aaaattatcg ataagttcaa
    23521 gtggactaaa gaagatatgg aatctatcat gttagatatg gataaaggta tggagcctgc
    23581 taaagcagct caaaaatgga ttaaaaacca caaaaaagaa gtttcagagt ggactaaata
    23641 gtatagaaaa aaacacgtct ctcttagagc gtgtttttta atgtgactta agtcgtttta
    23701 gctatttaat ataagtttta tattatttta atacatcaaa agtgagagaa tgaaagaaat
    23761 ggcaggttat aaagtatta
//
LOCUS       DATROQ010000024        23763 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.024, whole genome shotgun sequence.
ACCESSION   DATROQ010000024 DATROQ010000000
VERSION     DATROQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23763
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.024"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            80..2278
                     /gene="nrdD"
                     /locus_tag="VBC94_000844"
     CDS             80..2278
                     /gene="nrdD"
                     /locus_tag="VBC94_000844"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEO6979126.1"
                     /translation="MMVLERERFMTESDIKVIKRDGRLVSFDKYKIYTALLKASNKVI
                     KMSPLVEAKLEMIADHVIAEIYNRFKDNIKIYEIQNIVEHKLLEANEYAIAQEYINYR
                     TQRDFERSQATDINFSIGKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAQLNYQKHLKDAKEWVIEDKQEDYAR
                     AKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTNWFEREIQKAILKIRIQGL
                     GSEHRTAIFPKLIFTLKKGLNLEENSPNYDIKQLALECATKRMYPDVLSYDKIIDLTG
                     SFKAPMGCRSFLQGWRDANGQDVTSGRMNLGVVTVNLPRVAMESNGDMDKFWEIFNER
                     MSIARDALVYRVERVKEAIPANAPILYQYGAFGERLGKYENVDRLFNHRRATVSLGYI
                     GLYEVASVFYGGDWEDNHQAKAFTVDIVRKMKQLCADWSDEYDYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKNPTPFEKLDFEKIYPETGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYQCQFEGDFTPTDRGFTCPNCG
                     NSDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSSIKNQGN"
     gene            2353..2496
                     /locus_tag="VBC94_000845"
     CDS             2353..2496
                     /locus_tag="VBC94_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979127.1"
                     /translation="MGKYQLDYKGQAQVQKFHEKHSTGENANQKSRLKDLRKQFLEKA
                     KKK"
     gene            2509..3441
                     /locus_tag="VBC94_000846"
     CDS             2509..3441
                     /locus_tag="VBC94_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6979128.1"
                     /translation="MLQLGIVGLGGISQKAYLPYMRQVTGVHWHLFTRQKQILEEVNM
                     LFGSSTAYDSLDSLAEHPLDGVFIHVATSAHFDIAKLFLKKGIPVFMDKPLTEDYTST
                     KALYDLAKDHKTFLMAGFNRRFAPRIMEMKKVEDKNHIRTFKNAVNAPADFQYKLFDM
                     FIHPLDTALFLTNNVVKRGYFVTKRDGNKILQVSVTLETDSEIIEASMNLQSGSRREI
                     IEIESPEVTYSLDDLSNLSVIDGFDRRAIGFGSWASTLEKRGFEPMIDAFIQAITTGV
                     NPISPKSSLLSHFICDQINKANAPFGMLNLKIME"
     gene            3450..3941
                     /locus_tag="VBC94_000847"
     CDS             3450..3941
                     /locus_tag="VBC94_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6979129.1"
                     /translation="MKLRRPVLEDKEEILAMYKEFQKESSSMDGGFYEPTTHFEDWLD
                     HNLNMELGIGVPDNFVPYIQFVSFDNDNNAIGFLNLRLRLNDTLLEKGGHIGYSIRPR
                     QRGKGYAKEQLKLGIEQAHLKNINEILVTCHVDNDASKSVILANGGILEDCRHQTERY
                     WIT"
     gene            4014..4631
                     /gene="nrdG"
                     /locus_tag="VBC94_000848"
     CDS             4014..4631
                     /gene="nrdG"
                     /locus_tag="VBC94_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEO6979130.1"
                     /translation="MSEEASWNTPKPGEWKSEELSHGHIIDYKAFNFVDGEGVRNSLY
                     VAGCMFHCKGCYNTATWSFRAGIPYTKELEDQIMKDLAQPYVQGLTLLGGEPFLNTGI
                     LLPLLQRIRRELPEKDIWSWTGYTWEEMMLETQDKLEMLSLIDILVDGRFDQSKRNLM
                     LQFRGSSNQRIIDVQKSLKEGEVVIWEGLNDGDNSYEQVKRDYLL"
     gene            complement(4988..5719)
                     /gene="yaaA"
                     /locus_tag="VBC94_000849"
     CDS             complement(4988..5719)
                     /gene="yaaA"
                     /locus_tag="VBC94_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEO6979131.1"
                     /translation="MIKILIPTAKEMKVCQNIARPKLSAQTQIIIDYFSTLTVSDLED
                     IYRINTSAARCEAQRWQDFKCKQLTLNPAIKLFNGLMYRNIKRHNLSTSEAQFMENSV
                     FITSALYGIIPAMTLISPHRLDFNTKIKINNNSLKVFWRENYDTFMQSDDIMVSLLSN
                     EFKTVFSPKERQKLIHLNFIEDRDGQLKTHSTISKKARGKCLTSMMENNCQTLEHLKQ
                     LRFDGFCYDNELSDSKQLTFVKKQT"
     gene            complement(5762..6622)
                     /locus_tag="VBC94_000850"
     CDS             complement(5762..6622)
                     /locus_tag="VBC94_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6979132.1"
                     /translation="MPYPYKANHSIESITLKVNDLENLVNFYSDIIGLTVIDKSSTRA
                     LLGVNQKIPLIILEKTELEKHSTYGLYHTAILVPDEYHLSLALNHLLSQHIPLEGGAD
                     HGYSNAIYLSDPEGNGIEIYNDKDISMWDIRESGQIIGITERLDIDNLLDSLVNVPNN
                     YKLSEKTRIGHIHLSVKDAKISSKLYQNVFGLDEKFAIPTASWIASGNYHHHLAFNNW
                     AGPNLSKNQENRPGISLLTIAYNDDNLFRDSLKKAQLYQLTFLEKQDHYYIIEDFDGI
                     RIKVVLKPEN"
     gene            6808..7605
                     /locus_tag="VBC94_000851"
     CDS             6808..7605
                     /locus_tag="VBC94_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6979133.1"
                     /translation="MVRLIFSDIDGTLINSNFKVTPKTRQGIKQIVAQGATFVPISAR
                     MPEAITPIMEQIGIDSYIISYNGALIQDMQQKTIASHTMDGQVALQVCSYVSKHYSKI
                     AWNVYRYHEWYSCDKENEWVQKEEEIVGLQSKEMSLMELEKQDRIHKLLLMGEPSLMG
                     ELENTLKAQYPHLSIAQSAPYFIEIMAPGIEKGKSAKTLADYLDISLADSIAFGDNYN
                     DLNLLEIVGKGFVMGNAPKDLQERIGNVTQDNDNDGIYYALVEYMEN"
     gene            8070..9032
                     /locus_tag="VBC94_000852"
     CDS             8070..9032
                     /locus_tag="VBC94_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6979134.1"
                     /translation="MKKITKMVISSVLASVLMTACSQSSQNGQSDVKHIGILQYVEHP
                     SLTATRKGFIKELAKEGYKDGKNIKIEYKNAQGDQSNIQSISEKLIKDNKLVLGIATP
                     AAQSLTTVSTETPILFTAVTDPVSAELVKSMKKPEGLATGTSDMSPIKKQVSLLRKVM
                     PKVKRVGIMYTTSERNSEVQVKQAKKIFQEAGIKTSVKGISSTNDVQDTAKSLMSKTE
                     VLFVPTDNIIASSVTLLGNLSKELKVPVVGGSADMVPSGLLFSYGADYEALGCQTARQ
                     AVKILKGKDVAKVPSEYPQNLKVVVNEDMAKELGIDVSSIKNNK"
     gene            9048..9944
                     /locus_tag="VBC94_000853"
     CDS             9048..9944
                     /locus_tag="VBC94_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6979135.1"
                     /translation="MLDIILSGISQGLLWSIMAIGVFITFRILDIADLSAEGAFPMGA
                     AVCALCIVNDINPIVATIAGMLGGMLAGLVSGFLHTKMKIPALLTGIITLTGLYSINL
                     LVLGRSNVSFALKNTLVTMVTRLGLNKLSAVLLIGIVCVGLVILILYLFLNTQLGLAL
                     RATGDNEAMGQANSIKVDRMKMLGYMIGNGLIALSGALLAQNNGYADLNMGVGTIVIG
                     LASIILAEVMIKYLPLGKRLWSIVLGSVLYRMIIVFILTTDIDAQMIKLVSAILLALI
                     LYVPELRAKLGVKASKTLTKEG"
     gene            9949..10752
                     /locus_tag="VBC94_000854"
     CDS             9949..10752
                     /locus_tag="VBC94_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6979136.1"
                     /translation="MSLLELVNLHKTFEKGTVNENHVLRGLDLTIEDGDFISVIGGNG
                     AGKSTLLNCIAGLIPIDQGTITLDNQSITKDSVEKRSKGISRVFQDPRMGTATNLTIE
                     ENMAIAHKRGNKRHIFRQSVTDDDRQLFKKSLSQLGLGLENRMKTDAAFLSGGQRQAL
                     TLAMATLVRPKLLLLDEHTAALDPKTSDMVMELTQKVIEEQRLTALMITHNMEHAIAY
                     GNRLVMLYHGKIVVDVKGEAKRNLTVAELMELFHKNSGQQLIDDALVLG"
     gene            10927..11211
                     /gene="groES"
                     /locus_tag="VBC94_000855"
     CDS             10927..11211
                     /gene="groES"
                     /locus_tag="VBC94_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEO6979137.1"
                     /translation="MLKPLGDRVIISFVETEEKSVGGFVLAGASHDTTKTAKVLAVGD
                     GIRTLTGELVAPSVAEGDTVLVENGAGLEVKDGNEKVTVVRESDIVAVVK"
     gene            11307..12929
                     /gene="groL"
                     /locus_tag="VBC94_000856"
     CDS             11307..12929
                     /gene="groL"
                     /locus_tag="VBC94_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEO6979138.1"
                     /translation="MAKDIKFSADARSAMVRGVDILADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPIGIRRGIETAVSAAVEELKEIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVSELENPYI
                     LITDKKISNIQEILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVVTEDLGLDLKDATMQVLGQSAKVTVDKDSTVIVEGA
                     GDSSAIANRVAIIKSQMEATTSDFDREKLQERLAKLAGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVSGGGTALVNVIEKVAALKLNGDEETGRNIVLRALEEPVRQ
                     IAYNAGYEGSVIIERLKQSEIGTGFNAANGEWVDMVTTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPEAPTAPAMDPSMMGGF"
     gene            complement(12961..13325)
                     /locus_tag="VBC94_000857"
                     /pseudo
     CDS             complement(12961..13325)
                     /locus_tag="VBC94_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13338..14075)
                     /locus_tag="VBC94_000858"
     CDS             complement(13338..14075)
                     /locus_tag="VBC94_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6979139.1"
                     /translation="MPKNELNNKLNKLKHVQVYNKIFNMIQDGTYSPGMQLPSEPELA
                     GQLNVSRATLRKSLALLQEDHLVKNIRGKGNFIRENSSNLSENGYENRQHPIKTCLTS
                     KITEVELEFRVEVPAEAITASLKQETPVVVIADRWYHTDDGPLAYTLSFIPIELISDA
                     EISLHDTKQLLNFIEEGIYHEGISSHSQSHLGYATSGNFSATKYTLSDHGQFILIQET
                     IFNQEKILMCNKHYVPIEHFELSITSH"
     gene            14234..15013
                     /gene="udp"
                     /locus_tag="VBC94_000859"
     CDS             14234..15013
                     /gene="udp"
                     /locus_tag="VBC94_000859"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEO6979140.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            15034..16236
                     /locus_tag="VBC94_000860"
     CDS             15034..16236
                     /locus_tag="VBC94_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEO6979141.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQVVSVVSTGVTKVINCGQAGLNFVFGSLADSGTKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILFPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRGTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            16266..16937
                     /gene="deoC"
                     /locus_tag="VBC94_000861"
     CDS             16266..16937
                     /gene="deoC"
                     /locus_tag="VBC94_000861"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEO6979142.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIKNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVDEGVKIKAAGGISSLEDAEKFIALGASRLGTSRIIKIVKNQKV
                     EEGTY"
     gene            17004..18215
                     /locus_tag="VBC94_000862"
     CDS             17004..18215
                     /locus_tag="VBC94_000862"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO6979143.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGDPGNFTRT
                     ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHDMGHNKSNSHGIDTL
                     IKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPEIISAMCDKDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADISATVADNFGVDTA
                     MIGESFLQDLV"
     gene            18385..20025
                     /locus_tag="VBC94_000863"
     CDS             18385..20025
                     /locus_tag="VBC94_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979144.1"
                     /translation="MFDFFKKKVVKVCLVIFGIVLVSLLSLGFFYFSKGQVLSRFVAA
                     RSRTSGQAFDNIKEYMVWSDTGESITNDEANYANFEPLSKSEARKLGQEIKEGNKNDS
                     MYLKRVGSRLGIFPDYRIANKPMSLTLKTNVPKLDVLLNQKKVATSNSDHFSVTVERL
                     PRTHYTASLEGTSDGKEIKLKKDYDGKNQTIDLSVAFKSFTVTSNLMDGNLYFGDNRI
                     AKLKDGSYSVENYPVTDGSKAYIKKVFNDGEITSHKQKLISIADNQTIKLDVDGLLNE
                     KEAGQKLITAFNQLILYVSTGQDPQTLGTVFEKGAENDFYKGLKESIKAKFVTDNRKA
                     SHFTIPNIVLNKMTQVGKESYQVNFAADYDFNYDKSTDPDKKTYGHIIQNLTGNFIMK
                     KSGNSYLISNDGKKDITVAKETNKVKADPVFIFPENLVGSWKGEVEDGTVTMTFDKDG
                     KVTQKKVYKDSKSKESNHSAKVTKLEDKGNGLYLYQYESGTDTTTFVTGGIGGLKVKY
                     AYGIKIEGNKIIPVIWQTSSDGEFDYHKPLLSKPLTKQ"
     gene            complement(20050..20934)
                     /locus_tag="VBC94_000864"
     CDS             complement(20050..20934)
                     /locus_tag="VBC94_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6979145.1"
                     /translation="MSFSLKIRNPYGEHTVKELLEDYFLIPRKIRHFLRVKKHVLINN
                     EFINWQTVVQENDTITLIFDDEDYPTKKIPLGRAELIDCLYEDEHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSAYSGQTCYVVHRLDMETSGAVLFAKNPFILPLINQRLERKEIWR
                     EYWALVEGKFSPKHQVLRDKIGRNRHDRRKRIIDSKNGQHAMTIIDVLKYIQNSSLIK
                     CRLETGRTHQIRIHLSHHGHPLIGDPLYNPSSNNERLMLHAHRLTLSHPLTCETISVE
                     APSSTFEKVLNNYKKGVG"
     gene            20978..23299
                     /locus_tag="VBC94_000865"
     CDS             20978..23299
                     /locus_tag="VBC94_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6979146.1"
                     /translation="MKLFDKFIDLFRVDEDNDEMTRKNEQETREETSNLDGEEVYDID
                     DITRPSKSQYQRGIRHQKENAKSRLEWLQKVDRYLPSPKNPIRRFWRRYRIGKLLFIA
                     LMAFILIFGSYLFYLSKTATVSDLQSALKTTTTIYDKNKEYAGKLSGQKGTYVELNAI
                     SDHLKNAVIATEDRTFYENNGVNFKRFFLAVATLGKFGGGSTITQQLAKNAYLSQDQT
                     IKRKAREFFLALELTKKYSKAEILTMYLNNSYFGNGVWGVEDASRKYFGTSAANLTVD
                     EAATLAGMLKGPEVYNPYYSVENATNRRDTVLAAMVDAGQLTKSQAKEAASIGMKNRL
                     ADTYAGKINDYKYPSYFDAVVNEAIDTYGISEKDIVNNGYKIYTALDQNYQSGMQKTF
                     DDTSLFPVSDYDGQSAQGASVALDPKTGGVRGLVGRVQSTKDAQFRSFNYATQSKRSP
                     ASTIKPLVVYSPAIASGWSIDKELPNKVQDFHGYKPSNYGGIETESIPMYQALANSYN
                     IPAVYTLDKLGINKAFTYGRKFGLNMSSANKELGVALGGSVTTNPLEMAQAYSTFAND
                     GIMHRAHLITRIETANGKLVKQFTDKPKRVISRSVASKMTSMMLGTFSNGTAINANVY
                     GYTMAGKTGTTETDFNPNLSGDQWVVGYTPDVVISQWVGFKNTDKHHYLTDSSAGTAS
                     NIFSTQASYILPYTKGSSFTHIENAYFQNGIGSVYNAQDASNTTNQESRSIINDLKDS
                     ASKAAQDISRAVEDSNFQEKVKDAWNSLKDYFR"
     gene            23348..23500
                     /gene="rpmG"
                     /locus_tag="VBC94_000866"
     CDS             23348..23500
                     /gene="rpmG"
                     /locus_tag="VBC94_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6979147.1"
                     /translation="MAQKKASLACTECGSRNYSIGVSSTPKPTRLEVNKFCKYCKKYT
                     IHKETR"
     gene            23536..23709
                     /gene="secE"
                     /locus_tag="VBC94_000867"
     CDS             23536..23709
                     /gene="secE"
                     /locus_tag="VBC94_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEO6979148.1"
                     /translation="MFVKGIFQVLRDTTWPNRKQRWKDFISILEYTVFFTIVIYIFDK
                     LLAAGVMDLINRF"
BASE COUNT         7748 a         3662 c         4854 g         7499 t
ORIGIN      
        1 gtgtctgagc aaaaaacttg acacaatata ttgatttttt ttctttgcat gctataattg
       61 tcactggagg atatttcaca tgatggtatt ggaaagagaa cgatttatga cagaatctga
      121 tatcaaagtg attaaacgtg acgggcgttt ggtcagcttt gataagtata aaatctacac
      181 ggctctatta aaagctagta ataaagttat caaaatgtct cctttagttg aggctaaact
      241 tgagatgatt gcagatcatg tcatcgctga gatctacaat cgttttaaag ataacatcaa
      301 gatatatgaa atacaaaata tcgttgaaca taaattattg gaagctaatg agtacgctat
      361 tgcacaagaa tatatcaatt atcgtaccca acgcgatttt gagcgttcac aggctacaga
      421 tatcaatttt tctattggaa aattaattaa taaagatcaa actgttgtta atgagaatgc
      481 taataaagac tctgatgtgt ttaataccca acgtgattta acagcaggta ttgtagggaa
      541 atcaattggt ctgaaaatgt taccttctca tgtagcaaat gcacatcaaa agggtgatat
      601 ccattaccat gatttagatt acagcccata cactcccatg acaaattgct gtttaatcga
      661 ttttaaggga atgcttgcaa atggttttaa gattggtaat gcagaagttg agagtccaaa
      721 atctattcag acagcgactg ctcagatttc acagattatt gcgaacgttg cttcaagtca
      781 atatggtggc tgtacagcag atcgcattga tgagttttta gctccttatg cacagttaaa
      841 ttatcaaaaa catttaaaag atgcaaaaga atgggttatt gaagataaac aagaggacta
      901 tgcgcgcgct aaaacacaaa aagatatata tgatgccatg caatctttgg agtatgagat
      961 taatacttta tttacttcaa atgggcaaac gccatttact tcgcttggtt ttggattggg
     1021 aactaattgg tttgaacgag aaattcaaaa agcgattttg aaaattcgga ttcaaggttt
     1081 aggaagcgag catcgtactg ctatttttcc aaaattaatt tttacgctta aaaaaggtct
     1141 taatttagaa gaaaatagcc ccaattatga tattaaacaa ttagcattag agtgtgcgac
     1201 aaaacggatg tatccagatg tcctttctta tgataaaatt attgacttaa cgggctcttt
     1261 taaagcccct atgggctgtc gttccttcct acaagggtgg cgagatgcaa atggtcagga
     1321 tgtaacatct ggtcgtatga atcttggagt tgtaacagtt aatctaccgc gtgttgccat
     1381 ggagtctaac ggtgatatgg ataaattctg ggagatattt aatgaacgta tgagtattgc
     1441 tagagatgcc ttggtttatc gtgtggaacg tgtcaaagag gctatcccag caaatgcgcc
     1501 cattctttac cagtatggag catttggaga acgtttagga aaatatgaga atgttgatcg
     1561 actctttaat caccgccgtg caacggtgtc tcttggttat attggtttat atgaggtggc
     1621 gagtgttttt tatggtggtg actgggaaga taatcatcaa gctaaagcat ttactgtaga
     1681 catcgttcgt aagatgaagc agttatgtgc agattggtct gatgagtatg attatcattt
     1741 ttcagtttat tcgacaccat cggagagctt gactgaccgg ttttgtcgtt tagatactga
     1801 aaaatttggt atagtaactg atattactga taaggaatac tacactaatt cattccatta
     1861 tgatgtgcgt aagaatccaa caccttttga aaaactagat tttgagaaaa tctatcctga
     1921 aacgggtgca tcaggaggat ttattcacta ctgtgaatac cctgttttgc aacagaatcc
     1981 aaaagcttta gaagcagttt gggactatgc ttatgatcgg gtaggttatc taggtactaa
     2041 tacaccaatt gataagtgtt accaatgtca atttgaaggg gattttacac cgacagatcg
     2101 aggctttact tgtccaaatt gtggtaacag cgaccctaag acagtagatg ttgtcaaacg
     2161 tacatgtggt tacttaggta atccacaggc tcgtccgatg gttaatggtc gtcataagga
     2221 aatctctgct cgtgtaaaac atatgaacgg atcaagtatt aaaaatcaag gtaattaatt
     2281 aaaacctcta ttaaaacctc tggtctatta tatgttaaaa taagaagata aaatagaagg
     2341 agttattaag atatggggaa ataccaatta gattacaaag ggcaagcaca agtacagaag
     2401 ttccatgaga agcactctac tggagagaat gctaatcaaa aatcacgtct aaaagattta
     2461 aggaaacaat ttttggaaaa ggcaaagaaa aaatagaagg aggatgctat gttgcaactt
     2521 ggaattgtcg gtttaggagg tatttcacag aaagcttatc taccttatat gcgtcaagtg
     2581 acaggtgtgc actggcatct ttttacacgc caaaaacaga ttttagaaga agttaatatg
     2641 ctttttggtt cttctacagc ttatgacagt ttagatagtc tagccgagca tcctttggat
     2701 ggagtcttca ttcatgtcgc gactagtgct cactttgata tagcaaagct ttttttaaaa
     2761 aagggtattc cagtttttat ggataagcct ttgacagaag attatacttc aactaaagca
     2821 ttgtatgatt tggcgaagga tcataaaaca tttttaatgg caggttttaa tcgtcgcttt
     2881 gcaccacgta ttatggaaat gaaaaaagta gaagataaaa accatattcg cacctttaaa
     2941 aatgctgtca atgcaccagc ggattttcaa tataagcttt ttgatatgtt tatccatcct
     3001 ttagatacgg cgctattttt gacaaataat gttgttaaaa gaggatactt tgtgactaaa
     3061 cgtgatggga ataagatatt acaagtatca gtaacgctag aaacagacag tgagataata
     3121 gaagcaagca tgaacctgca gtcggggtct cgtcgagaaa taattgaaat agagtcgcca
     3181 gaagtaacct attcattaga tgatttgtca aatctttctg ttattgatgg ctttgaccgt
     3241 cgtgctatcg gttttggttc ttgggcaagc actctagaga aacgtggttt tgaaccaatg
     3301 attgatgctt ttattcaagc tatcactaca ggtgtcaatc ctatatcacc aaagtctagt
     3361 ttattgagtc attttatttg tgaccagatt aataaagcga atgctccttt tggaatgctt
     3421 aacttgaaga taatggaata ggaaacaata tgaaattacg gcgcccagtg ttagaagata
     3481 aagaagaaat tttagccatg tataaagagt ttcaaaaaga aagtagttca atggatggtg
     3541 gtttttatga accaactacg cattttgaag actggctaga tcataaccta aatatggagt
     3601 tagggatagg tgttcctgat aattttgttc catatattca gtttgtgtct tttgacaatg
     3661 acaataatgc tataggtttc ttaaatttac gtttaagact aaatgatact cttttagaaa
     3721 aaggtggaca tattggttat tccatacgac ctcgccagcg tggtaaaggt tatgctaaag
     3781 aacagttgaa gctaggaatt gagcaagcac atctaaaaaa tatcaatgaa atacttgtga
     3841 catgccatgt agacaatgat gccagcaagt cagtaatatt agctaacggt ggtattttag
     3901 aagattgtcg tcatcagaca gagcgttatt ggataaccta agatagtact ttaagataat
     3961 aataaaaaga ccagttagtt ctggttcttt tcatatcaat agagaggtta gaaatgtcag
     4021 aagaagcaag ttggaatact ccaaagcctg gggagtggaa aagcgaagaa ttaagtcatg
     4081 gccatatcat tgattataaa gcttttaatt ttgtagatgg tgaaggtgtt agaaattcac
     4141 tctacgtagc tggatgtatg ttccactgta aagggtgtta taatactgct acatggtcct
     4201 ttcgagccgg aatcccttat acaaaagagc ttgaagatca aattatgaaa gatttggcac
     4261 aaccttacgt ccaaggttta acgttattag gtggagagcc gttcttaaat acaggtattt
     4321 tgctgccact tcttcagcgt atccgacgtg aattgccaga aaaagatatt tggtcatgga
     4381 cggggtatac ttgggaagaa atgatgctgg agactcaaga taaattggag atgttatctt
     4441 tgattgatat tttagtggat ggtaggtttg atcaaagtaa gcgaaattta atgctacaat
     4501 ttagaggatc ttcaaatcaa agaatcattg atgtacaaaa atctttaaaa gaaggtgaag
     4561 tcgtgatttg ggaaggatta aacgatggtg acaatagtta tgaacaagtc aaacgagatt
     4621 atttacttta gtgtcaaaaa ttaactttta aatagaaatt gaaagttatg tttatccaca
     4681 gcttgtttat aagtatttga cagtctgtga caagatgttg acgtttatta ttgttaattt
     4741 aacaagtatt tttgaaatct aaaggtatat acaatgacat tactgttaag taatcaactt
     4801 tatataaaca gattatcctc tgtctatccc catagttatc cacaagttgt tgataacaat
     4861 tattttttgt taatattatt tgaaaccttg atatagagag gtttttagac cttacaagtt
     4921 tgtcaaaaag caagagatac gattatctct tgctttttgt gtgaataaat tgtggataaa
     4981 tagttactta agtctgtttt ttaacaaaag ttaattgctt agagtctgag agttcattat
     5041 cataacagaa gccatcaaag cgtaattgct ttaagtgttc tagagtttgg caattatttt
     5101 ccatcataga agttaaacac tttcctcttg ctttttttga tatggtagag tgggttttta
     5161 attggccatc tcgatcttca ataaagttaa gatgtattag tttttgacgc tctttaggag
     5221 aaaagacggt tttaaattca ttagaaagca aagaaaccat aatgtcatct gactgcataa
     5281 aagtatcgta attttcacgc caaaaaactt taagagagtt attgttgatt ttgattttag
     5341 tgttaaaatc taaacgatga ggggatatta gtgtcatagc aggaatgatg ccataaagag
     5401 cactagtaat aaaaacagaa ttttccataa attgtgcttc acttgtggat aagttgtgcc
     5461 ttttgatatt gcggtacata agaccgttga ataacttaat agcaggattc agagtaagtt
     5521 gtttacattt aaaatcttgc catctttgag cctcacaacg agctgcactt gtattaattc
     5581 tgtagatatc ctctaagtct gaaacagtta aagtagaaaa ataatcaatt ataatttgcg
     5641 tttgtgcact cagtttaggc cttgcaatgt tttgacacac tttcatttct tttgcagttg
     5701 ggatcaaaat ttttatcata ttgacattat gtaaaatgaa gagtcaaaag tcaagtgatg
     5761 gttaattctc tggttttaga acaactttaa ttcggatgcc atcaaaatct tcaataatat
     5821 aataatgatc ttgtttttca aggaaagtca gttggtaaag ttgcgctttt tttaagctat
     5881 ctctaaaaag gttgtcatca ttgtaagcaa ttgtcaataa ggaaattccg ggtctatttt
     5941 cttggttttt agaaagattt ggaccagccc aattattaaa ggctagatga tgatggtagt
     6001 taccggatgc aatccaacta gctgtcggga ttgcaaattt ctcatcaagg ccaaaaacat
     6061 tttgatagag ttttgagcta atcttagcat ctttaacact caaatgaata tgaccaatac
     6121 gagttttttc agaaagctta tagttattag gaacgttgac aaggctgtca agtaaattgt
     6181 caatatctag tctttcagta ataccaataa tttgtccact ctctctgata tcccacatag
     6241 agatatcctt atcattataa atttcaattc cattaccttc aggatcactt agatagatag
     6301 cattgctata gccatggtca gctccacctt ctagagggat atgctgagat aataaatgat
     6361 ttagagctag actgagatgg tattcatctg gtactaaaat agcagtgtga taaaggccat
     6421 aggtactatg cttttcaagt tcagtttttt caaggataat aagagggatt ttttgattta
     6481 cccctagtaa ggctctagtg cttgatttgt caataacagt taacccaatg atatcgctat
     6541 agaagtttac gaggttttcg aggtcattta cttttaaggt gattgattca atggaatgat
     6601 tggctttgta tggatacggc atagttaatc ctcctattat tactatatag taacatatta
     6661 aaccgttcat tgagagaaat aaactaattt gttttgaaat atagcttata ttagtagtct
     6721 cattagacca gacgaagttt atttgcgaaa caaaaattta agtgttagac taaagtaagc
     6781 gcgtacaatt atattttgga ggataatatg gtaaggctaa tatttagtga tattgatggt
     6841 actttaatta acagtaattt taaagtaaca cctaagacac ggcagggaat taaacaaata
     6901 gtagctcaag gggctacttt cgttcccata tcagctagga tgcctgaggc aattactccg
     6961 attatggaac aaattggaat agattcttat attatttcct ataatggggc tttaatacaa
     7021 gatatgcaac aaaaaacaat tgcttcccac acaatggatg gtcaagttgc cttacaagtt
     7081 tgttcttatg taagcaagca ttattcgaaa attgcatgga atgtctatcg ttatcatgaa
     7141 tggtattcct gtgataaaga aaatgagtgg gtccaaaaag aggaggagat tgttggttta
     7201 caatctaaag agatgagttt gatggagtta gagaagcaag ataggattca taaacttctc
     7261 ttgatgggag aaccatctct gatgggagaa ttggagaaca ctttgaaagc tcaatatcca
     7321 catttatcaa tcgcacaatc tgctccttac tttattgaaa taatggctcc aggtattgaa
     7381 aaaggaaaat ctgctaaaac tttagcagac tatttagata tttctcttgc agactctatt
     7441 gcgtttggtg ataactataa tgatttaaat ctgcttgaaa ttgtgggtaa aggttttgtc
     7501 atggggaatg ctcctaaaga tttacaagag agaattggaa atgtaactca ggataatgat
     7561 aacgatggta tttattatgc tttagtagag tatatggaaa attaaggata gcaaatctaa
     7621 atttccttaa aagtgtgtta tatcaacgaa tgactattat ttaaattatc tatctaacaa
     7681 gaattttcaa aaaaatcaaa tttaactatt gacaattatt tttaaatgac gtaaacttat
     7741 aggcaaccat aaacaaggag agaataatat gttaatcaaa tcgtctatta ttaaacaaac
     7801 aaatacttat tattacttta gtttctttag atagacgttt gtagcctcaa ggatacaaac
     7861 gtcacgcaaa gtttgtatct atgaagctaa agcattttat gatgaaagca gagccgatta
     7921 gatcatttca ataatctagt tggttaaggc aatatttttt cgccctcccg tgcacagagg
     7981 aattgagaga aataattaac caactagatg tagggtctag ttggtttttt gttttatgga
     8041 aaatttgatg gtattacaag gagatagtaa tgaaaaaaat aacaaagatg gtaattagca
     8101 gcgttttagc atctgttctg atgacagctt gttcacaatc ttctcaaaat ggtcaatctg
     8161 atgtaaaaca tattggtatt ttacaatatg tagaacatcc ctctctaact gccactagga
     8221 aaggatttat aaaagaatta gctaaagaag gctataaaga tggcaaaaat atcaaaattg
     8281 agtataagaa tgctcaaggg gatcagtcaa atatccaatc aatttctgaa aaactcatta
     8341 aagataataa attagtattg ggaattgcga ctccggcggc tcaatcgttg acaacggtgt
     8401 caacagaaac acctatttta tttacagcag tgacagatcc ggtatcagcc gaattagtaa
     8461 aatcaatgaa aaaacctgaa ggtttagcga ctggtacaag tgacatgtct ccaattaaaa
     8521 agcaagtatc ccttttgaga aaagtaatgc ctaaggttaa acgtgttggt attatgtata
     8581 ccactagcga gcgtaactca gaagttcagg taaaacaagc aaaaaaaatc ttccaagagg
     8641 cggggattaa aacaagtgtt aaaggtattt cttccactaa tgatgtacaa gatactgcta
     8701 agagtttaat gtctaaaaca gaggttcttt ttgtaccaac agataatatt atagccagct
     8761 cagtaacgct gttagggaat ctatcgaaag aattaaaagt gcctgttgtg ggaggttcag
     8821 cagatatggt accaagtggt ttgctctttt cttatggggc tgattatgag gctttaggtt
     8881 gtcaaacggc aagacaagct gttaaaatcc tcaaaggtaa agacgtcgct aaagttcctt
     8941 cagaatatcc tcaaaatcta aaagtagtag ttaatgaaga tatggcaaaa gagcttggaa
     9001 ttgatgtttc aagtattaaa aataataaat aatcagaaag gtattgtatg ttagatatta
     9061 tcttatccgg aatttcgcaa ggattacttt ggtcaattat ggcaattggc gtgtttatca
     9121 cttttcgtat cttagacata gccgatctct ctgcagaagg ggctttccct atgggggctg
     9181 cagtttgcgc cttatgtatc gttaatgata ttaatccaat tgtggcaacc atagcgggca
     9241 tgttaggtgg tatgttggca ggactagtat cgggttttct tcatactaaa atgaaaatac
     9301 cagccctcct aacaggtatt attacgttga caggacttta ctctattaat ttacttgttt
     9361 taggaagatc aaacgtctct tttgctctca aaaatacttt ggttacaatg gtaacaagat
     9421 taggtttaaa taaactttca gcagttttat taattggaat tgtttgtgta ggtttagtta
     9481 tcttaatttt gtatctcttt ttaaataccc aactaggatt agctttaaga gcgacaggtg
     9541 ataatgaggc tatgggacaa gccaatagta ttaaagtaga tcggatgaaa atgctaggat
     9601 atatgattgg gaatggcctg attgctttat ctggtgcact tttagcgcaa aataatggct
     9661 atgcagattt gaatatggga gtgggtacaa tcgttattgg attggcttca attatcttgg
     9721 cagaagtaat gataaaatac ttacctcttg gtaaaaggtt atggtcaatt gtattaggtt
     9781 ctgttttata ccgtatgatt attgttttca tattaacaac cgatattgat gcacagatga
     9841 taaagttggt ttcagcaatt ttgttggctc tcattttgta tgtcccagaa ttacgagcta
     9901 aactaggagt aaaagcctct aaaacgctta caaaggaagg ataagaacat gtcattactt
     9961 gaattagtca atctgcacaa aacttttgaa aaaggaactg tcaatgagaa ccatgtttta
    10021 cgtggtttag atttaacgat tgaagacggt gattttattt cagtaattgg aggtaacggt
    10081 gcgggaaaat caaccctcct taactgtatc gctggtctga tccctattga tcaggggaca
    10141 atcacattag acaaccaatc aattactaaa gactctgtag aaaaacgatc taagggtatt
    10201 agtcgagtct ttcaggatcc tagaatggga actgcaacaa atttgacgat tgaagaaaat
    10261 atggcaattg cacataaaag aggcaataag cgtcacattt ttagacagtc agtgactgac
    10321 gatgaccgtc aactatttaa aaagtctcta tcacaattag gtctaggctt agaaaatcga
    10381 atgaagactg atgctgcatt tttatctggc ggtcagcgtc aagccctcac gttagctatg
    10441 gcaactcttg ttcgtcccaa attattatta ctggatgaac atacagcggc tctagatccc
    10501 aaaactagcg atatggttat ggaattaaca cagaaagtga tagaagaaca acgattgaca
    10561 gccttaatga ttacacacaa tatggagcat gccattgctt atggtaatcg tctcgtgatg
    10621 ctctaccacg ggaaaattgt tgttgatgtt aaaggagaag caaagaggaa cttaacagtt
    10681 gcagaactaa tggaactttt ccataaaaac tcaggtcaac agttgataga tgatgccctt
    10741 gtcttaggtt aaaatataaa tttctctagt gaatggacag gataactagg gaaatttttc
    10801 ttgtttgaaa cgtgcctttt ctcttgacta attctgacca agtgatagaa tagtttttgt
    10861 agttagcact ctctttatag gagtgctaaa aaatcaaaat atatctcgta tggaggttcg
    10921 tttattatgt taaaaccatt aggtgatcgt gtgatcattt cgtttgttga aactgaagag
    10981 aaatctgtcg gtggttttgt tcttgcagga gcaagccatg atacgacaaa gacagccaaa
    11041 gttcttgctg tgggtgacgg tattcgtacc ttaacagggg aattagttgc acctagcgtt
    11101 gcagagggtg atactgtttt agtcgaaaat ggtgctggtt tggaagttaa agacggtaac
    11161 gaaaaagtga cagttgttcg cgaaagtgac attgttgctg tcgttaaata ggttttatta
    11221 agcaagaaag gatataggcc tattagagaa aagttgcttt gtaaagaaag tagactttgt
    11281 taaattttag gcctgggtat taaattatgg caaaagatat taaattttca gcagatgccc
    11341 gctcagcaat ggtgcgtggt gttgatattt tagctgatac agtcaaagta acattaggtc
    11401 ctaaaggccg taatgttgtt cttgaaaaag catttggttc tcctttaatt acaaatgatg
    11461 gtgtgacaat tgctaaagaa attgagctag aagatcactt tgaaaatatg ggagctaaac
    11521 ttgtgtcaga agtggcttca aaaactaatg atattgcagg ggatggcact acaactgcta
    11581 ctgttttgac ccaagctatt gtacgggaag gtcttaaaaa tgtaactgca ggggcaaatc
    11641 cgattggcat tcgtcgtggt attgaaacag ctgtttcagc agcagttgaa gagctaaaag
    11701 agattgcaca accagtttca ggcaaagaag ctattgctca agttgcggct gtgtcttcac
    11761 gttctgaaaa agttggggaa tacatttctg aagctatgga gcgcgtgggt aatgatggtg
    11821 ttatcactat tgaagaatcg cgaggtatgg aaacagagct tgaagttgtg gaaggaatgc
    11881 agtttgaccg tgggtacttg tcacagtata tggtaactga taacgagaaa atggtctctg
    11941 aacttgagaa tccgtatatc cttattacag ataagaaaat ttcaaatatc caagaaattt
    12001 taccattatt agaagaggtt cttaaaacaa atcgtccgtt gctaatcatc gctgatgatg
    12061 ttgatggaga agctctccca acgcttgttc ttaacaaaat tcgtggaact ttcaatgtcg
    12121 tagctgttaa agcgcctgga tttggtgatc gtcgtaaagc catgctggaa gatattgcta
    12181 tcctaacagg aggaactgtc gttactgaag accttggttt agacttaaaa gatgctacta
    12241 tgcaagtttt aggacagtct gctaaagtaa cagtagataa agattctact gttattgtcg
    12301 aaggtgccgg tgactcatca gcaattgcta atcgcgtagc catcattaag tcacagatgg
    12361 aggctacaac ttctgatttt gatcgtgaaa aattacaaga acgacttgct aagttagccg
    12421 gtggtgtagc agtaattaaa gttggtgcgg cgactgaaac agaattaaaa gagatgaaac
    12481 ttcgtatcga agatgcgtta aatgcaacgc gtgctgcagt tgaagaaggt attgtttcag
    12541 gtggaggtac ggctcttgtg aacgttattg aaaaagtagc ggcactgaaa cttaatggtg
    12601 atgaggagac tggacgtaat attgttcttc gtgctctcga agagcctgtt cgtcaaattg
    12661 cttacaatgc tggatatgaa ggttcagtta ttattgaacg tttaaaacag tctgaaattg
    12721 gtacaggatt taatgcggct aatggagaat gggtagatat ggttaccaca ggtatcattg
    12781 atcctgtcaa agtaacacgt tctgcacttc aaaatgcggc atctgtagca agtcttatct
    12841 tgactacaga agcagtagta gcaaataaac ctgaaccaga agctcctaca gctcctgcaa
    12901 tggatccatc tatgatgggt ggcttctaat cactaactta tcacaacata aaactcccct
    12961 ttaacaaagg ggagttttat gttgtgataa tagagatatg atagtcacta ccacttgcgg
    13021 taagaagatc ataaggatag gccatctaat atttgccagt agtgggagga aaaaagtagc
    13081 aaaacaatga ttgctattat cttagattga gattgataag ctaagaattg aattatagct
    13141 aatagaatta agtaactaat aagtataaat aacatagaaa agagcattcc gtacccagca
    13201 cccattgacg gttcctttgg aggggctaga aaataactta acttaaaata agcgccaact
    13261 attaggccca taattaattc ggtaaaagtt atgaaaaaag caacttttaa tagtctattt
    13321 tgcataggct tgcctcctta atgagaggta atggacaatt caaagtgctc aatgggaacg
    13381 taatgcttat tacacattag tattttctct tggttaaaaa ttgtttcttg tattaatatg
    13441 aattgtccat gatcggatag ggtgtattta gttgctgaaa aattgccaga tgtcgcatat
    13501 cccaaatggc tttgtgagtg gcttgatatg ccttcgtgat agataccttc ttcaatgaag
    13561 tttaacaact gtttagtatc atgtaaagaa atctcagcat ctgaaataag ttcgataggg
    13621 ataaaagaaa gtgtgtaagc taatggtcca tcatcggtgt ggtaccaacg atcagcaata
    13681 acaactacag gggtttcttg tttaagtgaa gctgtgatag cttctgcagg aacctctaca
    13741 cgaaattcta attccacttc tgtaattttg gaagttagac aagttttaat ggggtgttgt
    13801 ctattttcgt aaccgttttc agacaagttg ctagaatttt ctctaatgaa attacctttt
    13861 cctctgatat ttttaaccaa atggtcttct tgtaatagtg ctaatgactt tcgtaaagta
    13921 gctcgactaa cattaagttg ccctgctaat tcgggctcag aaggcaattg cattccagga
    13981 gaataagtac cgtcttgaat catgttaaaa atcttattgt agacttgcac atgctttagt
    14041 ttatttagtt tattatttag ttcattttta ggcattgtaa cctccgtata caagttagtt
    14101 taattgaaaa aattagaaaa atcaagttaa aaaataaaac gttttctttc gataaaagcg
    14161 tttacaaagg ttgtcttgta tgttattata caagtgtaaa aaaaatagac aagacattaa
    14221 ggaggtcttt atcatgcaga attattcagg tgaagtcgga ttgcaatatc acctacagat
    14281 tcgtccaggt gatgtaggtc gttatgttat catgccaggt gatccaaaac gttgtgcaaa
    14341 aattgcagaa cattttgata atgcagttct tgtagcagac agccgtgaat atgttactta
    14401 tacaggtaca ttaaatggtg aaaaagtcag tgttacttca actggtattg gtggtccatc
    14461 agcgtctatt gcgatggaag aattgaaact ctgtggcgct gataccttta tccgtgttgg
    14521 aacatgtggt ggtattgacc ttgatgttaa aggcggagac atcgttattg caactggagc
    14581 tatccgtatg gaaggtacaa gtaaagaata tgctccgatt gaatttcctg ctgtagctga
    14641 cttggaggta acgaatgctc tagttaatgc agctaaaaag cttggttaca caagtcacgc
    14701 aggagtagtt caatgtaaag atgcatttta tggacaacat gagccagaac gtatgccagt
    14761 tagttatgaa ttattaaata aatgggaagc atggaaacgt ctgggaacaa aagcgtctga
    14821 aatggaatct gcagctcttt ttgtagcagc aagtcatcta ggagttcgtt gtggatcaga
    14881 tttccttgtt gttggtaatc aagaacgcaa cgctttgggt atggataacc caatggctca
    14941 tgacacagaa gcagctattc aagttgctgt tgaagcactc cgcacactta ttgaaaatga
    15001 taaatcacaa taagcgaggt tttggaagtc atcatgcaat ttatttatag tattattggt
    15061 attttattgg tattaggaat tgtgtatgca atttctttca atcgtaagag tgtttctcta
    15121 agtttaattg gaaaagctct tatcgttcaa ttcattattg cgctaatctt agtacgtatc
    15181 ccactaggcc aacaagttgt tagtgttgtt tcaactggag ttactaaagt aatcaactgt
    15241 ggtcaagctg gtttaaattt tgtgtttggt tcattagcag atagtggaac aaaaactggt
    15301 tttattttcg ccattcaaac gcttggtaat attgttttct tatctgccct agttagtcta
    15361 ctttattatg taggaatcct tggatttgta gtaaaatgga taggtaaggg tgttggtaaa
    15421 attatgaaat cctcagaggt tgagagtttt gttgccgtag ctaatatgtt tcttggtcaa
    15481 acagacagtc caattttggt tagcaaatac ctaggtcgta tgactgatag tgagataatg
    15541 gttgtgttgg tatcaggtat gggaagtatg tcagtttcta ttcttggtgg ctacattgca
    15601 ttaggcattc caatggaata tctcttgatt gcttcaacaa tggttcctat tggcagtatt
    15661 ctcattgcta aaatcttatt tcctcaaaca gaacctgttc aaaaaattga tgacattaag
    15721 atggataata aaggtaataa cgccaatgtg attgatgcaa tcgctgaggg tgcaagcaca
    15781 ggtgcacaaa tggctttctc aattggtgct agtttgattg cctttgttgg tttagtttct
    15841 ttgattaata tgatgttaag tggattggga attcgcttag aacaaatctt ctcatatgtt
    15901 tttgctccat ttggttttct tatgggattt gaccacaaaa acattcttct agaaggaaac
    15961 cttcttggaa gtaagttgat tttaaatgag tttgtttcgt tccaacaatt gggtcaccta
    16021 atcaaatctt tagattatcg tacagcattg gtagcaacta tttcactctg tggttttgct
    16081 aatttatcaa gtttaggtat ttgtgtttca ggtattgctg ttctttgtcc agagaaacgt
    16141 ggcaccctag ctcgacttgt tttccgtgca atgattggtg gtattgctgt aagtatgctt
    16201 agcgccttta tcgtcggtat tgtaactcta ttctaaaagt tgacaaaaga aagaaggata
    16261 gtaacgtgga agtaaaagat attttaaaaa cggtagacca tactttgcta gcaacaacag
    16321 caacatggcc agaaatccaa acaattttag atgatgcaat ggcttatgaa acagcttcag
    16381 catgtattcc agcctcttac gtcaaaaaag cagcagagta cgtttcaggt aaattagcta
    16441 tttgtactgt tattgggttc ccaaatggct atagtacaac tgcagcgaag gtttttgaat
    16501 gtcaagatgc tattaaaaat ggtgctgatg aaattgacat ggtcattaat ttgacagacg
    16561 ttaaaaatgg ggattttgat actgttgaag aagaaattcg tcaaatcaaa gctgcatgtc
    16621 aagaccatat cttaaaagtt atcgttgaga catgtcaatt aactaaagaa gaacttatcg
    16681 aactttgtgg agttgtcaca cgttcaggtg cagactttat taaaacctct actggttttt
    16741 cgacagcagg tgctacattt gaagatgttg aagtgatggc aaaatatgtc gacgaaggtg
    16801 ttaaaattaa ggcagcaggt ggaatctcat cattggaaga tgctgaaaaa tttattgctt
    16861 taggagcttc acgcttgggt actagccgta tcatcaagat tgttaagaac caaaaagttg
    16921 aagaaggcac ttattaaata atataaagga aataggaagg atgctggtct gacttcctat
    16981 tttcaattga taaaggagaa gttatgtcac aatttgatcg cattcactta gttgtcttag
    17041 attcagtggg tattggagcg gcaccagatg ccaatgattt tgtcaatgca ggagttcctg
    17101 atggtgcttc agacacgctt ggccacattt caaaaacagt tggacttgcg gtgccaaata
    17161 tggctaaaat tggacttggt aatattcctc gtccccaagc gcttaaaacc gttcctgcag
    17221 aagaaaatcc aagtggctac gcgacgaagt tacaagaagt atcacttggt aaagatacaa
    17281 tgacagggca ttgggagatc atgggcttaa atattacaga gccatttgat actttctgga
    17341 atggttttcc agaagatatc atcacaaaga ttgaggattt ttcaggacgt aaagttattc
    17401 gtgaagcgaa taaaccttat tcagggacag ctgttattga tgacttcgga ccacgccaaa
    17461 tggaaactgg tgagctcatc atctatacct cagctgaccc agtcctacaa attgcagctc
    17521 atgaggatat catccctcta gaagaacttt accgtatttg tgaatatgct cgtagtatca
    17581 caatggagcg tccagctctc cttggtcgta ttatcgctcg tccatatgta ggtgacccag
    17641 gcaactttac gcgtacagcc aaccgtcacg actatgcagt atcaccattt gaagataccg
    17701 tattaaataa actagatcaa gctggcatcg atacttatgc tgttggtaaa atcaatgata
    17761 ttttcaatgg ttcaggcatt aatcatgata tgggacataa caaatcgaat agtcatggta
    17821 ttgatacttt gataaaaaca atggggttat cagagtttga aaaaggcttc tcctttacta
    17881 atttggttga ctttgatgct ctctatggtc accgtcgtga tcctcatggc tatcgtgatt
    17941 gtcttcatga gtttgatgag cgcctcccag agatcatttc agcaatgtgt gacaaagact
    18001 tacttttgat cactgccgat catggtaatg acccaactta tgcaggtaca gaccatacaa
    18061 gagaatacat tcctctctta gcctatagcc catctttcac aggaaacggc cttatcccag
    18121 tagggcattt cgcagatatt tcagcaacag ttgcggataa ctttggtgtt gatactgcga
    18181 tgattggaga gagcttcttg caggacttgg tataatcata gacaaaaatt ttagaggttg
    18241 aaatcggatt atggtttcaa tccttttttc gttttgttat aatagaaaaa tagcggttac
    18301 aagtacaaat acagctgttt tatcagtgtt gtaacttttc tttaagcaat ttactttaat
    18361 ataaagtgaa tgagaggata aggtatgttt gattttttta agaaaaaagt tgttaaagtg
    18421 tgcttagtta tttttggtat agttttagta tcacttctga gtctaggatt cttttatttt
    18481 tcaaagggac aagtacttag tcgttttgta gcagcacgtt ctcgtacgtc aggtcaagcg
    18541 tttgataata ttaaagaata catggtttgg tcagatacgg gagaatctat cacaaatgat
    18601 gaggccaatt acgctaattt tgagcctttg tctaaaagtg aggcgcgtaa attagggcag
    18661 gaaataaaag aaggaaacaa aaatgattca atgtatctaa agcgtgtagg tagtcgctta
    18721 ggtatattcc ctgattatcg tattgctaat aagcctatgt ctttgacctt gaaaaccaat
    18781 gttcctaaat tggatgtttt gttgaatcaa aaaaaggtcg cgacaagcaa ttcagatcat
    18841 ttttcagtaa cggtggaacg tttacctaga acccattata ctgctagcct tgaagggact
    18901 agtgacggaa aagagattaa actcaaaaaa gattatgatg gtaaaaacca aactattgat
    18961 ttatcggtcg cttttaaatc ttttacagta acaagtaatc ttatggatgg taatctttat
    19021 tttggtgata atcgtattgc taaattaaaa gatggtagct attccgtaga gaattatcca
    19081 gtgactgatg gttcaaaagc ttatatcaaa aaagttttta atgatggtga gataacctct
    19141 cataaacaaa aattaatctc tatcgctgat aatcagacaa tcaaattaga tgttgatggt
    19201 ttattaaatg aaaaagaagc tggacagaaa ttgataactg ctttcaatca attgatactt
    19261 tatgtatcaa ctgggcaaga ccctcagact ttaggaacag tttttgaaaa aggtgccgaa
    19321 aatgattttt acaaaggatt gaaagaaagt attaaagcaa aatttgtaac tgataatcgt
    19381 aaagcgagcc atttcactat tcctaatatt gtccttaata aaatgacaca ggttgggaaa
    19441 gaaagttatc aagttaattt tgctgccgat tatgatttta attatgataa atcaacagat
    19501 cctgataaga aaacatatgg ccacattatt cagaatttaa ctggtaattt tattatgaaa
    19561 aaatcgggaa atagttacct tatttctaat gatggtaaaa aagatatcac agtagctaag
    19621 gaaactaata aagttaaggc tgatcccgtt tttatttttc cagagaacct tgtaggaagt
    19681 tggaaaggtg aagttgagga cggtacagta acgatgactt ttgataagga tggtaaagtt
    19741 actcagaaaa aagtttacaa ggatagtaaa tctaaggaaa gtaaccattc tgctaaagtt
    19801 acgaagttgg aggataaggg aaatgggctc tacctttatc aatatgaatc aggtactgat
    19861 actacaactt ttgtaacagg aggtatcggt ggtcttaaag ttaaatacgc ctatggaata
    19921 aaaattgaag ggaataagat catcccagta atctggcaga catctagtga tggagaattt
    19981 gattaccata aaccactact cagtaagcca ttaactaagc aataatgatt aaaaggagtt
    20041 ggataattat tatccaactc cttttttata attgtttaaa accttctcga aagtagatga
    20101 aggggcctct acgctaatag tttcgcaagt taatggatgg gatagagtca atcggtgagc
    20161 gtgtagcatt aacctttcat tattagaaga agggttgtag aggggatctc ctattaaagg
    20221 atgtccgtga tgagataagt gaatgcgaat ttgatgggtt cttccggttt ccagtcggca
    20281 ttttatgaga ctactatttt ggatatactt caaaacgtca atgattgtca tagcatgttg
    20341 accgttttta gaatcaatga ttcgtttacg tctgtcatga cggttccgtc caattttgtc
    20401 tctcaaaact tgatgcttag gtgaaaattt tccttcaact aaagcccaat attcacgcca
    20461 aatttctttt cgttctaagc gttgattgat aaggggaagt ataaatggat ttttagcaaa
    20521 taaaacagct ccactggtct ccatatctag gcgatgaaca acatagcatg tttgtccaga
    20581 ataggcagat acatgattta acagtgctat ttcatttggt tggttaccgt gagttttcat
    20641 accttcaggt ttattaacga taataagatg ttcatcctca taaagacaat caataagctc
    20701 tgctctgccc agaggaattt ttttagtagg gtaatcctca tcatcaaaga ttaaggtaat
    20761 agtatcgttt tcttggacga cagtttgcca attaatgaat tcattgttta taagtacatg
    20821 ttttttaaca cgcaaaaaat gtctaatctt acgtggaatc aaaaaataat cttcaaggag
    20881 ttctttaacg gtatgttcac cgtatggatt tctaattttt aatgaaaatg acatggtttt
    20941 attatagcat tttattgaaa attgatataa taagtctatg aaattatttg ataagtttat
    21001 agacttattt cgtgttgatg aagacaacga cgaaatgaca agaaaaaatg aacaagaaac
    21061 tagagaagaa actagcaatt tggatggcga agaagtctat gatattgatg atattacccg
    21121 acctagtaaa tcccagtatc agcgaggcat tcgtcaccaa aaggaaaatg ctaaatctcg
    21181 acttgaatgg ctacaaaaag ttgatcgtta cctcccttct cctaagaatc ctatccgtcg
    21241 tttttggcgg cgctatcgta ttgggaagtt gctttttatc gccttaatgg cttttattct
    21301 tatatttggt agctatcttt tttatctttc taaaactgct acagttagtg atttacagag
    21361 tgctctaaaa acaaccacca ccatttatga taagaataaa gaatacgctg gaaaactttc
    21421 gggacaaaag ggaacttatg tagagttaaa tgctatttct gatcatctca aaaatgctgt
    21481 tatagcaact gaagatagaa ctttttatga aaataatggt gtaaacttta agcgattttt
    21541 tttggcagta gctacacttg ggaaatttgg cggaggctca actattaccc agcaattagc
    21601 aaaaaatgct tacttgtcac aggaccaaac gattaaacgc aaggcgcgtg aattcttttt
    21661 agcgctagaa ttgactaaga aatatagcaa ggctgagatt ctgaccatgt atcttaataa
    21721 ctcttatttt ggtaatggtg tttggggtgt ggaagatgct agtaggaaat attttggaac
    21781 cagtgctgct aatttgacag tagatgaggc tgctacactt gctggaatgc ttaaaggacc
    21841 agaagtttat aatccttatt attctgttga aaatgcaact aaccgaagag atactgtttt
    21901 ggcagcgatg gttgatgcag gtcaactaac caagtctcaa gctaaagaag ctgcttcaat
    21961 tggtatgaaa aatagattag cagataccta tgctggtaaa attaatgatt acaagtaccc
    22021 atcctatttt gatgctgttg tcaatgaagc cattgatact tatggtattt cagaaaaaga
    22081 tattgtcaat aatggctata agatttacac tgcattagat caaaattatc aatcaggaat
    22141 gcaaaaaaca tttgatgata ctagcctctt ccctgtatct gactatgatg gtcaatctgc
    22201 ccaaggtgct agcgtagctc tagatccaaa aactggtggt gtaagaggtt tagttggacg
    22261 tgttcaaagt actaaagatg cacagtttag aagttttaac tacgcgactc aatcaaagag
    22321 aagtccagcc tcaaccataa aacctttagt tgtttattca ccagctattg cttcaggatg
    22381 gtcaatagat aaagaacttc ctaataaagt tcaagatttt catggttata aaccaagtaa
    22441 ctatggtgga attgagactg aaagtatccc gatgtatcaa gcactagcta attcttataa
    22501 tattcctgct gtttatactt tagataaact aggaatcaat aaagctttta cttatggcag
    22561 gaagtttggt ctaaatatgt catcagcaaa caaagagcta ggagtggcgc taggtggaag
    22621 tgtaactact aatcctttag aaatggcaca agcttactca acttttgcaa atgatggtat
    22681 aatgcatcgc gctcatttga ttactcgaat tgagacagct aatggaaaat tagtaaagca
    22741 atttacagat aagcctaagc gagttattag tcgctcagtg gctagtaaaa tgactagtat
    22801 gatgttagga acattctcaa acggtacagc tattaatgcc aatgtttatg gttatacaat
    22861 ggcaggtaaa actggaacaa ccgaaacaga ttttaacccc aatttatctg gagaccagtg
    22921 ggtagtcggc tatacgcctg atgtcgttat tagtcaatgg gttggcttta agaacacaga
    22981 taaacaccat tatttgacag attctagtgc aggtacagct tcaaatatat ttagcacgca
    23041 agcttcatat atactaccgt atactaaggg gtctagtttt actcatattg agaatgctta
    23101 tttccaaaac ggtattggtt cagtttataa tgcacaagat gctagtaata caacgaacca
    23161 agaatcacgc tcaattatta atgatttaaa agattcggca agtaaggctg cacaagatat
    23221 ttctagagct gtggaagatt caaatttcca agagaaggta aaagatgctt ggaatagtct
    23281 aaaggactac tttagatagc ttgcaggatt atacaaatca tgttaaaata acacttatgg
    23341 aggcgttatg gcacaaaaga aagcaagcct agcgtgtaca gaatgcggaa gccgaaatta
    23401 ctcaatcgga gtaagtagca cccccaagcc aacacgacta gaagtaaata aattttgtaa
    23461 atattgtaag aaatatacaa tacacaaaga aacgcgttga tatagcgtaa caaactgcaa
    23521 aaaggagaac tgataatgtt tgtcaaaggg atttttcaag tattgcgtga cactacttgg
    23581 ccaaatcgaa aacaacgttg gaaagatttt atttccatct tggaatacac tgtatttttc
    23641 acaatcgtta tctacatctt tgacaaactg ttagctgcag gagtcatgga cttgattaat
    23701 cgattttaat aatagattat tttagataga aacaacccaa acccttagat tttactaagg
    23761 gtt
//
LOCUS       DATROQ010000025        23674 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.025, whole genome shotgun sequence.
ACCESSION   DATROQ010000025 DATROQ010000000
VERSION     DATROQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23674
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.025"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            913..2331
                     /locus_tag="VBC94_000868"
     CDS             913..2331
                     /locus_tag="VBC94_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HEO6979149.1"
                     /translation="MMTKETLAHYQKKIEQESEKKQSLDEHSWHVACFSRQEASIIGQ
                     GDVLFLIGLYHDLGKADRAFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKSRGVDKG
                     ERMTFNEMVGYVISAHHGMYDFCYCSDDAEYYSFNKFKNRINRDLDDYHYHEDIKGYA
                     IKLEEKLCDYGYKDLRELIDKAFDNYQQAMSSLNWQDNSEWDYYQSCMVRLYLSLLKN
                     ADILDTVNAYGLKISPMDKTERSSLKHSYLAAIEQKYASFGRPNNQLNTIRTEIAERV
                     KERGKRDSKGIYRLDLPTGAGKTNLSMRYAFHQLVHQDKSRFFYITPFLSVLEQNASE
                     IRKVTGDLGVLEHHSNMVKQANEDDDKDSLLSAYLIDSWDSQVVLTSMVQFFQTLFKT
                     KSANLRRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFFNKVMDTTIVLCTATQPAYD
                     SSEIDHRICYGGNLGELAEIVD"
     gene            2602..3561
                     /locus_tag="VBC94_000869"
     CDS             2602..3561
                     /locus_tag="VBC94_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6979150.1"
                     /translation="MLKVRYGVVSTAKVAPRFIEGVRLAGNGEVVAVSSRTLESAQAF
                     ANKYHLPKAYDKLEDMLADESIDVIYVATINQDHYKVAKAALLAGKHVLVEKPFTLTY
                     DQANELFTLAESCNLFLMEAQKSVFIPMTQVIKKLLASGEIGEVISISSTTAYPNIDH
                     VTWFRELELGGGTVHFMAPYALSYLQYLFDATITHASGTATFPKGQSDSQSKLLLQLS
                     NGVLVDIFLTTRLNLPHEMIIYGTEGRLIIPHFWKTTHAKLVRNDTSARTIQVDMVSD
                     FEKEAYHVSQMILEGQRVSHIMTPQLTLSGVKIIEGLYRSWGK"
     gene            3722..4624
                     /locus_tag="VBC94_000870"
     CDS             3722..4624
                     /locus_tag="VBC94_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6979151.1"
                     /translation="MFSSDFGFEIINVDDLSLQEEKFLPSELLAYARDENESSFVRDI
                     EGHLTLVYQLLDTQGHVDDVRHVPRVIPVTLFLKEDGLFVLANHKNIILVKKALNRVE
                     KVDSPKHLLLSLVTAFSKQYFDVLDTISEERDKLINDLRKRPNKSNLARLANLQSGTV
                     HLMMGTKQNFEMLTDLQNIEQDKENTRNEKMQLQDAIIEARQLSNMCSLNSQVFQELS
                     SSYNNVLSNNLNDNVTTLTIISIGISIIAMVTSFYGMNVKLPFDSVDAVWVLIILITT
                     IITIMLSIVMYIYVHKEDNHPKHK"
     gene            4784..5848
                     /locus_tag="VBC94_000871"
     CDS             4784..5848
                     /locus_tag="VBC94_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEO6979152.1"
                     /translation="MSIESDFFRKKRFIFSSLEEFGFIKSDQEYIYCQTFMDNDFKAI
                     ITISLDGKIAGKVIDSALEEEYLPLRAANYNGSFVGEVRSAYMAILGDISDSCCKDLL
                     FTKDQSNRLAEKIATTFEDSVDYPFAKHPQYASYRVSGKWYALLFPLKMGKLENVPAQ
                     LSEEEVEVLNIKVNPQDMEILLQKEGIYPSYHMSKKTWVSIVLDNTLSDIEIFKLVSD
                     SRKLVSPNKKSNSEPEFWIIPANPKFYDIDKEFAEHQLIDWTQKGKIAVGDYVFIYIT
                     APIRALRYVCQVIERDLKTSTYLGARDVKAYMRLKLIRQFEDSTYPVAFLAEHGVKTV
                     RGPRRMTKELRQVMAKTILE"
     gene            5963..6955
                     /locus_tag="VBC94_000872"
     CDS             5963..6955
                     /locus_tag="VBC94_000872"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEO6979153.1"
                     /translation="MKKSFIHQQQEISFVKNTFTQYLIDKLEIVEVQGPILSQVGDGM
                     QDNLSGIEHPVSVKVLNIPEAEFEVVHSLAKWKRHTLARFGFNEGEGLFVHMKALRPD
                     EDSLDPTHSVYVDQWDWEKVIPDGRRNLDYLKETVEKIYKAIRLTELAVEARFDIESI
                     LPKRITFIHTEELVEKYPDLSPKERENAIAKEYGAVFLIGIGGELADGKPHDGRAPDY
                     DDWTTPSENGFKGLNGDILVWNEQLGTAFELSSMGIRVDEDALKRQVVLTGDEDRLEF
                     EWHKTLLRGFFPLTIGGGIGQSRLAMFLLRKKHIGEVQSSVWPKEVKDTFENIL"
     gene            complement(7000..7449)
                     /locus_tag="VBC94_000873"
     CDS             complement(7000..7449)
                     /locus_tag="VBC94_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001893380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transport accessory protein"
                     /protein_id="HEO6979154.1"
                     /translation="MIKKLLLTLIAILCLTTLKSNLSSADTMTISQQQYQHMITNSDL
                     IAITDKALVSKSQQSEATIFIGRPTCQYCRAFLPKLLKSQATLHSKIYYLDSQKYKGK
                     RLKSFFKKHHITTVPNLAHYQRGKMTKYLVQGSQATPQQIQTFLSQK"
     gene            7415..8119
                     /gene="rsmD"
                     /locus_tag="VBC94_000874"
     CDS             7415..8119
                     /gene="rsmD"
                     /locus_tag="VBC94_000874"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEO6979155.1"
                     /translation="MAIKVNNNFLIIMGTSFLLLLAYHIKKEGKEILSSLFVISNFFD
                     NSTLFFVIIVVMRVVAGTFGGRPLKTLDGKTTRPTTDKVKGAIFNMIGPFFEGGRVLD
                     LFSGSGSLAIEAISRGMDQAVLVEKDRRAQVVIQENIAMTKSPEQFQLLKIEANRALE
                     QLTGQFDLVLLDPPYAKEEIVKQIQIMDSKGLLGDDIMIVCETDKSVDLPEEIASFGI
                     WKQKIYGISKVTVYVR"
     gene            8131..8421
                     /locus_tag="VBC94_000875"
     CDS             8131..8421
                     /locus_tag="VBC94_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979156.1"
                     /translation="MDYKLVMIILILVILIYLLLRLHYLEELLKHYHFKNDEGVCSLS
                     RTKNPLYLLMAMITFLPNLINWIANGFEAIDIVRLIPVVVFLWLYYCGDDDE"
     gene            8418..8903
                     /gene="coaD"
                     /locus_tag="VBC94_000876"
     CDS             8418..8903
                     /gene="coaD"
                     /locus_tag="VBC94_000876"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEO6979157.1"
                     /translation="MTKKALFTGSFDPVTNGHLDIIERASYLFDHVYIGLFHNLEKQG
                     YFSIECRKKMLEEAIRQFKNVSVLVAQDRLAVDLAREVGAKYFVRGLRNSQDFDYEAN
                     LEFFNKQLADGIETVYLSTSPSLSPISSSRIRELIHFKASVKPFVPKSVFREVEKMSE
                     E"
     gene            8893..9966
                     /locus_tag="VBC94_000877"
     CDS             8893..9966
                     /locus_tag="VBC94_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEO6979158.1"
                     /translation="MKNKDPKRKHKSLLGLLKWWIIGFAFLLLVLASLVVRLPYYLEM
                     PGGAYDIRSVLKVNKKADKAKGSYNFVAVSVSQATPAQVLYAWLTPFTELSSKEETTG
                     GFSNDDYLRINQFYMETSQNESIYQALKLANKQVSLTYKGVYVLNLAKNSTFKDRLHL
                     ADTVTGVNGKSFKNSSQLIKYVAALHLGDKVKVQYTSQGKKKESVGKVIKLSNGKNGI
                     GIGLTDHTEVSSDVPVDFNTEGVGGPSAGLMFTLAIYDQLVKEDLRKGRKIAGTGTIE
                     QNGHVGDIGGAGLKVVSAAKKGMDIFFVPNNPIDKNAKKGKTKVQTNYQEAKAAAKRL
                     GTKMKIVPVQNVQQAIDYLKKTK"
     gene            10041..11374
                     /locus_tag="VBC94_000878"
                     /pseudo
     CDS             10041..11374
                     /locus_tag="VBC94_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
     gene            11443..12021
                     /locus_tag="VBC94_000879"
     CDS             11443..12021
                     /locus_tag="VBC94_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEO6979159.1"
                     /translation="MRKEVTPEMLNYNKYPGPQFIHFENIVKSDDIEFQLVINEKSAF
                     DVTVFGQRFSEILLKYDFIVGDWGNEQLRLRGFYKDASTIRKNSRISRLEDYIKEYCN
                     FGCAYFVLENPNPRDIKFDDERPHKRRKSRSKSQSSKSQTRNNRSQSNANAHFTSKKR
                     KDTKRRQERHIKEEQDKEMTSAKQHFVIRKKK"
     gene            12072..13178
                     /gene="rlmN"
                     /locus_tag="VBC94_000880"
     CDS             12072..13178
                     /gene="rlmN"
                     /locus_tag="VBC94_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEO6979160.1"
                     /translation="MPKKTDTPDYKPSIYSLTRDELIAWAIEHGEKKFRASQIWDWLY
                     KKRVQSFDEMTNISKDFIALLNENFVVNPLKQRIVQESADGTVKYLFELPDGMLIETV
                     LMRQYYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLNNGEITAQIMLVQKYFDERGQG
                     ERVSHIVVMGIGEPFDNYTNVLKFLRTVNDDNGLAIGARHITVSTSGLAHKIREFANE
                     GVQVNLAVSLHAPNNELRSSIMRINRSFPLEKLFAAIEYYIETTNRRVTFEYIMLNGV
                     NDTPENAQELADLTKKIRKLSYVNLIPYNPVSEHDQYSRSPKERVEAFYDVLKKNGVN
                     CVVRQEHGTDIDAACGQLRSNTMKRDRQKAKVGR"
     gene            13178..13693
                     /locus_tag="VBC94_000881"
     CDS             13178..13693
                     /locus_tag="VBC94_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEO6979161.1"
                     /translation="MLKRLFTEDGELTKISRRFVWMLVVIYCLIIVRMCFGPQIMIEG
                     VSTPNVQRFGRIVALLVPFNSFRSLDQLTSFKEIFWVIGQNVVNILLLFPLIIGLLSL
                     KPSLRKYKSVILLAFLMSLFIECTQVVLDILIDANRVFEIDDLWTNTLGGPFALWSYR
                     NIKGWLLTIRK"
     gene            13889..15634
                     /locus_tag="VBC94_000882"
     CDS             13889..15634
                     /locus_tag="VBC94_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6979162.1"
                     /translation="MSIIKNLWWFFKEEKKRYLIGILSLSLVAVLNLIPPKIMGSVID
                     AITTGKLTRPQLLWNLLGLVLSALAMYGLRYIWRMYILGTSYKLGQVVRYRLFEHFTK
                     MSPSFYQKYRTGDLMAHATNDINSLTRLAGGGVMSAVDASITALVTLITMFFTISWQM
                     TLIAVIPLPLMALATSKLGRKTHETFKESQAAFSELNNKVQESVSGVKVTKSFGYQEQ
                     EIASFQEINQMTFVKNMRTMTYDVMFDPLVLLFIGASYVLTLAMGAFMISKGQVTVGD
                     LVTFVTYLDMLVWPLMAIGFLFNMVQRGSVSYNRINSLLEQESDITDPLNPIKPVVNG
                     TLRYDIDFFRYDNEETLADIHFTLEKGQTLGLVGQTGSGKTSLIKLLLREHDVTQGKI
                     TLNKHDIRDYRLSELRQLIGYVPQDQFLFATSILENVRFGNPTLSINAVKEATKLAHV
                     YDDIEQMPAGFETLIGEKGVSLSGGQKQRIAMSRAMILDPDILILDDSLSAVDAKTEH
                     AIVENLKTNRQGKSTIISAHRLSAVVHADLILVMRDGRVIERGQHQELLNKGGWYAET
                     YASQQLEMEEAFDEV"
     gene            15624..17363
                     /locus_tag="VBC94_000883"
     CDS             15624..17363
                     /locus_tag="VBC94_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979163.1"
                     /translation="MKSNQWQVFKRLISYLRPYKWFTVLALSLLLLTTVVKNIIPLIA
                     SHFIDHYLTNVNQTAVLILVGYYSMYVLQTLIQYFGNLFFARVSYSIVRDIRRDAFAN
                     MERLGMSYFDRTPAGSIVSRITNDTEAISDMFSGILSSFISAIFIFTVTLYTMLMLDI
                     KLTGLVALLLPVIFILVNVYRKKSVTVIAKTRSLLSDINSKLSESIEGIRIVQAFGQE
                     ERLKTEFEEINKEHVVYANRSMALDSLFLRPAMSLLKLLAYAVLMSYFGFTGVKGGLT
                     AGLMYAFIQYVNRLFDPLIEVTQNFSTLQTSMVSAGRVFDLIDETGFEPSQKNTEAFV
                     REGNIEFKNVSFSYDGKKQILDNVSFSVKKGETIAFVGATGSGKSSIINVFMRFYEFQ
                     SGQVLLDGKDIRDYSQEQLRKNIGLVLQDPFLYHGTIKSNIKMYQDITDQEVQDAAEF
                     VDADQFIQKLPDKYDSAVSERGSSFSTGQRQLLAFARTVASKPKILILDEATANIDSE
                     TEQIVQDSLAKMRQGRTTIAIAHRLSTIQDANCIYVLDRGKIIESGNHESLLDQKGTY
                     YRMYQLQAGMMEV"
     gene            17491..18057
                     /locus_tag="VBC94_000884"
     CDS             17491..18057
                     /locus_tag="VBC94_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEO6979164.1"
                     /translation="MLLLVDNYDSFTYNLKQYLSVYKEIFVIKNDDPNLFLLAESAEA
                     IVLSPGPGHPKDAGKILELINQFIGQKPILGICLGHQALAECLGGRLNLANHVMHGKQ
                     SWVTINDHTSLFKGIDSPTQVMRYHSLVVTDLPENIAIIARSNEDNEIMAFHCPSLKV
                     YAMQFHPESIGSIDGMKMIENFLTLIND"
     gene            complement(18125..18663)
                     /locus_tag="VBC94_000885"
                     /pseudo
     CDS             complement(18125..18663)
                     /locus_tag="VBC94_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857893.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
     gene            complement(18664..19656)
                     /gene="bioB"
                     /locus_tag="VBC94_000886"
     CDS             complement(18664..19656)
                     /gene="bioB"
                     /locus_tag="VBC94_000886"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEO6979165.1"
                     /translation="MSFQTNYIHLADEILSGKTSISYEQALEILNSDENWWEIYAAAL
                     YLKNQVSRNNIRLNVLLSAKQGLCAENCGYCSQSKESTADIDKFGLLPQNVILKQAIV
                     AHQNGASVFCIAMSGTKPSKREIEQLCQVIPEIKKSLPLEICLTAGFLDREQLHQLKQ
                     AGIDRINHNLNTPEENYPNIATTHSFKDRCDTLERIHNEDIDVCSGFICGMGESDEGL
                     ITLAFRLKELNPYSIPVNFLLAVEGTPLGKYNYLTPIKCLKIMAMLRFVFPFKELRLS
                     AGREVHFENFESLVTLLVDSTFLGNYLTEGGRNQHTDIEFLEKLQLNHTKKELI"
     gene            19781..20296
                     /locus_tag="VBC94_000887"
     CDS             19781..20296
                     /locus_tag="VBC94_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979166.1"
                     /translation="MKKLFKKAIAMCRDLPLNFVIVELSLYQREDGSSDNIKWISKRI
                     LEVTVTDQQWIYHLRMLNLPKLKPMSLASSDCPAREEKASLISIEECQKFTSLVRDKN
                     PFHQGEAAIVPAALLLEKIMEVRNGKIKEIHLTFLSPVRVNQEFSVQVVDNTVIIFQH
                     QILCVRGTYEE"
     gene            20286..21401
                     /locus_tag="VBC94_000888"
     CDS             20286..21401
                     /locus_tag="VBC94_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEO6979167.1"
                     /translation="MKNRDVYIGFGLRTPIGIKGKQFKHYRPELLGAHLLNQIKKIES
                     ESNIDSIICGNTVGTGGNIGRLMTLFSDYESYIPVQTIDMQCASSSSALFFGYLKISA
                     GINEKVLVGGIESSSLQPMRRYAKEDNRNGEYTVAQFSPDSYAETVMLEGAQRVCQKY
                     GFRREMLDKLAFLSHKRALTAKQGGYLEEVILPMEGMRDQGVRKLKEAFFQKLPRLME
                     NSPLLTIGNVCLMHDAAAFLTLQSQKTEFRIVHIVEVAGDPKLSPELVHTATEKLLTE
                     THTKISDYDAIEWNEPFAAIDALFNHYYPEEREKFNIFGGALAYGHPYACSGIINILH
                     LMQALKYKNKPMGLTAIAGAGGVGMAISIEYLGVKNA"
     gene            21394..22623
                     /locus_tag="VBC94_000889"
     CDS             21394..22623
                     /locus_tag="VBC94_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEO6979168.1"
                     /translation="MLESLKTIVKTNSDKKLFDGDLQVSYGEFYNLVRQDMASQDNRK
                     HVISTHSLLNQLVRFVSKLCQKALPIICKPNLTHNEISRLEKEVQYAPQLADFGVLSS
                     GTTADAKLLWRSFASWSDFFSIQNAYFSVTSNSRLFIQGDFSFTGNLNLALSLLLLGG
                     TLVVTQKNSVKYWQTLWEKTGVTHLYLLPSYLKLVEQYSKETALDNKTIITSSQYVSD
                     SLLEGLYIKHPKVSVKIFYGASELNYVSWYDGRDIRDKPQYVGEIVPNVAVRIKEGRI
                     FVKTPYSICGLSSEYCAGDYGELIDGKLYLFGRGDDWCNQSGIKLYLPRLIEKIKTCP
                     YIKDAVAFTKESQSHGQESHCCIVLIENQMQQECLKWLSEHFEKKYGFKHYHIVSKIP
                     LMPSGKIDYQQLKRQLA"
     gene            22736..23368
                     /gene="nth"
                     /locus_tag="VBC94_000890"
     CDS             22736..23368
                     /gene="nth"
                     /locus_tag="VBC94_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEO6979169.1"
                     /translation="MLSKAKSRYIIREIIKLFPDAKPSLDFTNVFELLVAVMLSAQTT
                     DAAVNKVTPALFERFPNPLVLAQADPKEIEPYISKIGLYRNKARFLNQCAKQLIEHFD
                     GKVPQTRQELESLSGVGRKTANVVMSVGFGIPAFAVDTHVTRICKHHQICKQSASPLE
                     IEKRVMEVLPPEEWLAAHQSMIYFGRAICHPKNPKCDQYPQLYHFPDNLK"
     gene            complement(23369..>23674)
                     /locus_tag="VBC94_000891"
     CDS             complement(23369..>23674)
                     /locus_tag="VBC94_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001884316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEO6979170.1"
                     /translation="LWILFTISTIILTPITKVSIVLCLFGILAEVVDINIGSGDFLYL
                     ATIGLSLPLHQMLFIIQIGAWLGIIYCLVMRKMKKTIAFLPFLSIAYIIVTSYSLLF"
BASE COUNT         7693 a         3638 c         4719 g         7624 t
ORIGIN      
        1 ctagagatgt tgtaatgatt cataatatta gtagtatttt ctattttcta tcaatttggg
       61 tttgtttagt agttttaaca ttccttaaaa acttaatttt ataacgagca aacaaaaata
      121 tatgctaatc tacttctttt tatgcgtaaa gatatatttt aaaatgacaa tcagattata
      181 aaggaacata atgctaaaaa tgaatagaca tagaaagtga agatacatga ggactttata
      241 aatagcagga aagtagatac tagttagata tagtagtgca agatagatga gaaaaaataa
      301 cgaatacttt aacttggtca taacaattcc attctaatgg tagtaagcat gcataaaatg
      361 taggttttat catactactt gaatccagca actagataga gggggttaca tacgataaga
      421 cagtagtatt ttaaaccgat cggttgactt attgatactg tttagggaac aagtgttttg
      481 ttgtcaatgt taaatttaat ttagcatttt gatggtatca ccgcaagaat gaaaggaaaa
      541 gttgtatgtg ctgaaaaaac taatgcttgg cgatttagaa cacacgttta ctagtcgaga
      601 tgacaaaaaa taagtaccaa atgtaaaggt cgatattatc agcattgtca gcgttaggtg
      661 ttataaaaat ttaacatctt ccaattaagc taggtttaag tttcctaagt tgaactacgc
      721 ctagccgaaa aataatttct tttttacaga actcctaaca aggattttga attgtcaaga
      781 ttcttgtttt tttggaaagt catttagagg ctttttgggg gaagtattaa ctattgtatc
      841 cgtttacaat tcctttcaaa aatgttagta tatcattgag ggatatgagt tatggataag
      901 ggagagagga tgatgatgac taaggaaacg ttggcacatt atcaaaagaa aatagaacaa
      961 gaatctgaaa aaaagcagtc tttagatgag cactcatggc atgtggcctg ttttagtcga
     1021 caggaagcat ctataattgg tcaaggagat gtgctttttt taattggtct ataccacgat
     1081 ctaggcaaag ctgatcgagc ctttcaagat aaattattaa ataatccaaa tcgacatgtt
     1141 gatcattctt atgcaggggc aaaatactta tgctctatta ttgggcctca tctaaaaagt
     1201 cgaggggttg ataaaggtga gagaatgaca ttcaatgaaa tggtgggtta tgtcatctcg
     1261 gctcatcatg ggatgtatga tttttgctac tgttctgacg atgctgaata ttatagcttt
     1321 aataaattta aaaaccgtat caacagagac ttagatgatt atcactatca cgaagatatt
     1381 aaagggtatg ctataaaatt agaagaaaaa ttgtgtgatt acggctacaa agatttaagg
     1441 gagcttattg ataaagcttt tgataattac caacaagcta tgtcttcctt aaactggcaa
     1501 gataatagtg agtgggatta ttatcagtct tgtatggtga gactttactt gtcgctctta
     1561 aaaaacgctg atattttgga cacagtaaat gcctatggtc ttaaaataag tcctatggat
     1621 aaaacagagc gatcctctct aaaacattct tatttagcag ccattgaaca aaaatatgct
     1681 agctttggac gaccaaacaa tcagttgaat actattcgga cagaaattgc tgagcgtgtt
     1741 aaagaaagag gtaaacggga ttccaaaggg atctatcgct tagatttacc gacaggagct
     1801 ggcaagacta atcttagtat gcgctatgcg tttcaccaat tagttcatca agacaaatca
     1861 agattttttt acataacgcc ctttctttcg gttcttgagc aaaatgcttc cgaaattaga
     1921 aaagttacgg gtgaccttgg cgttctagaa caccattcca atatggttaa acaggctaat
     1981 gaagatgatg acaaggacag tttattgtca gcttatctta ttgatagctg ggatagtcaa
     2041 gtagtcttga cttctatggt tcaatttttc caaacgcttt ttaaaacaaa atcggctaat
     2101 ttgagacgtt tttcaagttt gattaatagt gttgtgattc tagatgaagt tcagtcactg
     2161 cctattgaag tcaccacttt gtttaattta acgatgaatt tttttaataa agttatggat
     2221 acaaccatcg ttctttgtac agcaacacaa cctgcttatg attcttcaga gattgaccat
     2281 cgtatctgtt atggagggaa tttgggagaa ttagctgaaa tagtggattg aaataagttt
     2341 atacgttgct tcatcaccaa gttttacgtc gcacccattg cgggtgcgtg gattgaaata
     2401 taagttcagc aacttcacga gaaaatgttc ctcgtcgcac cctttgcggg tgtgtagttt
     2461 caactatttt aaatctttca aattatgcta ttaaaattgt ctaactttta acagagatgc
     2521 gattgatttg tattaatccc taaaaagata aattgctagt tcttatgcta taataactct
     2581 atcaatgtca aaggagtttt tatgttaaag gtaagatatg gtgttgtgtc aacggcaaag
     2641 gtggcgcctc gttttattga aggagtccgc ttagcaggaa atggtgaagt tgtggctgta
     2701 tcgagtcgaa ctcttgagtc tgcgcaagct tttgccaaca aatatcatct cccaaaggct
     2761 tacgataagt tagaggatat gttagcagat gagtcgattg atgtgattta tgtggcaaca
     2821 attaatcaag accattataa agttgctaaa gcagctctgt tagcgggaaa acatgtgttg
     2881 gtagaaaagc ctttcacatt gacttacgac caagcaaatg aattattcac tttggcagaa
     2941 agttgtaatc tgtttttgat ggaagcacaa aaatcggtat ttattccgat gacacaagtg
     3001 attaaaaaat tacttgcctc tggtgaaata ggagaagtga tctccatctc atctacaaca
     3061 gcctacccaa acattgacca tgtaacatgg tttagagaac ttgagttagg tggaggaaca
     3121 gtgcatttta tggcgcccta tgccctgtct tatttacaat acctatttga tgcgactata
     3181 actcatgcta gtgggactgc gacttttcca aaaggtcaaa gcgatagtca atccaaacta
     3241 ctcttgcaat tatcaaatgg ggtcctcgta gatatcttct tgacaacacg tttaaattta
     3301 ccgcatgaaa tgattattta tggaactgaa ggtcgtctaa tcattccaca tttttggaaa
     3361 accactcatg caaaattggt gagaaatgat actagtgcgc gaacgattca agttgacatg
     3421 gttagtgatt ttgagaaaga agcttatcat gttagtcaaa tgattttaga agggcaaagg
     3481 gtaagtcata tcatgacgcc tcagttgacg ctgtcaggtg ttaagattat agaaggctta
     3541 taccgttcat gggggaaatg atgctggcta tctagcatat taataaggag tactagatgt
     3601 tattttagta gtcctttttt gttttgctct cataaggtta attagattta aaagggtttt
     3661 ctacttgttt tttctcgatt tttgtttcat aatgtagaca agtttagagt gaggtgcttc
     3721 tatgttttcg agcgattttg gatttgagat aattaatgtt gacgatctat ccttacaaga
     3781 ggagaaattt ttaccttcag aattgctggc ttatgcacgt gatgaaaacg agtcttcttt
     3841 cgtacgtgat attgaggggc atttgacact tgtttaccag cttttggata cgcaaggtca
     3901 cgttgatgat gttcgtcatg tgccgagagt gataccagtg actctatttt tgaaagaaga
     3961 tggcttgttt gtattagcta atcataaaaa tattatcctt gtaaaaaaag cacttaatcg
     4021 cgttgaaaag gtagattcgc cgaaacacct cttattatca ttagtgactg ctttctcaaa
     4081 gcaatatttt gatgttttag atactatttc tgaagaaaga gataaactaa tcaatgattt
     4141 gcgtaaaaga ccaaacaaat cgaacttggc aaggctggca aatttacaaa gtggaacagt
     4201 acacttaatg atgggaacta agcaaaattt tgaaatgctg accgatttac aaaacattga
     4261 gcaagataaa gagaatacta gaaatgaaaa gatgcaactt caagatgcta ttattgaagc
     4321 aagacaatta tctaacatgt gttccttaaa ttcacaggtt ttccaagaac tttcttcttc
     4381 ttataataat gtcctcagca ataaccttaa cgacaatgta actaccttaa caattatttc
     4441 cattggtatt tccattattg ctatggttac cagcttttat ggaatgaatg ttaagttgcc
     4501 ttttgatagt gttgatgctg tctgggtatt gattatttta attacgacta tcattactat
     4561 tatgctctct attgttatgt acatttatgt ccataaagag gataatcatc ccaaacataa
     4621 atagagaatt aataagaggg tgctatcaat tagtttagtt ttaagcatgg caacatgctt
     4681 tttttgtaca aagaaactag aattcatctt tgactttgag cttgctttct aactcttaaa
     4741 acgacataat agaggataat gtacagtgaa tagagaggtt actatgtcaa tagaatcaga
     4801 ttttttccgg aaaaaacgtt ttattttttc atcgcttgag gagtttggat ttatcaaatc
     4861 tgaccaagag tatatctatt gtcaaacatt tatggataat gactttaaag ctattatcac
     4921 gatttcattg gatggaaaga tagctggtaa ggttattgat tcggcacttg aagaagaata
     4981 tttaccgctt cgagcagcaa attataatgg ttcgtttgtt ggcgaagtac gatcggctta
     5041 tatggctata ttaggtgata tttcagattc ttgttgtaag gatttattat ttaccaaaga
     5101 tcaaagtaat cgtttagcgg agaagatagc tacaactttt gaagattcag ttgattatcc
     5161 atttgcgaag cacccacaat atgcttctta tcgtgtatca ggtaaatggt atgctcttct
     5221 ttttccattg aagatgggga agctagaaaa tgttccagct caactgtcag aagaagaggt
     5281 tgaagttttg aatattaaag tcaatcctca agatatggaa atactcttac aaaaagaagg
     5341 tatttatcct tcttatcata tgtctaaaaa gacttgggta tctatcgttt tagacaatac
     5401 tttgtctgat atagaaattt ttaaattggt atcagatagt cgtaagcttg tttctcccaa
     5461 taaaaaatca aattctgagc cagagttttg gattattcct gctaatccta aattttacga
     5521 tattgataag gagtttgcag agcatcaatt gattgattgg actcaaaaag gaaagattgc
     5581 ggtgggagat tatgtgttta tttatattac agcacctatt cgtgctttgc gctatgtctg
     5641 tcaagtgata gaacgtgacc ttaaaacaag tacttactta ggagcaagag atgtcaaagc
     5701 ttatatgcga ctcaaactga taaggcaatt tgaagattct acctatcctg tagcattttt
     5761 agcagaacac ggcgttaaga ctgtgagagg accacggcga atgaccaagg aattgcgaca
     5821 agtcatggca aaaacaattc tagagtaatg actttcagaa aaaaacttgt aaaaaagtca
     5881 gaataattct acaattcctc ttccattttg ttacttgaag gagtataata gctctaacat
     5941 tttataattt aaggaggaac atatgaagaa aagctttatt catcagcaac aggagatttc
     6001 ttttgtcaaa aatactttta cacaatattt gattgataag ttagaaatcg tcgaagtgca
     6061 aggacctatt cttagtcagg tcggggatgg tatgcaagat aatttatctg gtattgaaca
     6121 tcctgtatcg gtgaaagtcc ttaatatacc tgaagctgaa tttgaagtcg ttcactcatt
     6181 ggctaaatgg aaacgtcata ctttggcaag atttgggttt aatgagggag aaggtctttt
     6241 cgttcatatg aaagctcttc gtccagatga agattctctt gatccaacac actcagttta
     6301 cgtagatcag tgggactggg agaaggtcat tcctgatggt agacgtaatt tggactacct
     6361 aaaagaaact gtggaaaaaa tctacaaagc tatccgccta acagagcttg ctgtggaagc
     6421 tcgttttgat attgaatcca ttttaccaaa gcgtattacc tttattcata cagaagaatt
     6481 ggtagaaaaa tacccagact taagccctaa agagcgtgaa aacgccatcg ctaaggagta
     6541 tggcgcagtc tttttgattg gtattggtgg agagttagca gatggtaaac cacatgatgg
     6601 tcgtgcaccg gactatgatg actggacaac accatcagaa aatggtttta aaggtctcaa
     6661 tggtgatatt ttagtatgga acgaacagct tggaactgcc tttgaactat catcaatggg
     6721 aattcgagtt gatgaagatg ctcttaaacg tcaagttgtt ctcaccggtg atgaagatcg
     6781 tttagagttt gagtggcata agacgctatt acgtggtttc ttccctctga ctattggtgg
     6841 gggaattggt caatcacgat tagctatgtt cttgcttcgt aaaaaacata ttggagaagt
     6901 acagtcaagt gtctggccaa aagaagtcaa agataccttc gaaaatatat tataaaaaat
     6961 cagccaagca gttttgtttg gctgatttta acaataagtt tatttttggc ttaagaaagt
     7021 ttggatttgt tgaggtgtag cttggcttcc ttgaacaaga tatttagtca tttttcctcg
     7081 ttgataatgt gctaaatttg gaacagtggt aatatgatgc tttttgaaaa aactcttcaa
     7141 tcgtttccct ttgtattttt gactatcaag gtagtaaatt tttgaatgaa gtgtggcttg
     7201 agattttaat aattttggta agaaagctct acaatattgg caagttggac gtcctataaa
     7261 aatagtcgct tcagattgtt gacttttgct tactaaggcc ttatctgtga tggcgattaa
     7321 gtcactattt gttatcatgt gttggtattg ttgttgagaa atagtcatgg tgtcagcgct
     7381 acttaagttt gattttagcg ttgtaaggca taatatggct attaaagtta ataataattt
     7441 tttaatcatt atgggaacct cgtttctctt actgctagct tatcatatca aaaaagaagg
     7501 aaaagagata ctatcttctc tgtttgttat aagcaatttt tttgataact ctaccttatt
     7561 ttttgttata atagtggtta tgagagtagt agcaggaaca tttggagggc gtcctttaaa
     7621 aacgcttgat ggaaaaacaa ctcgtccaac aactgataag gttaaaggcg ctatttttaa
     7681 tatgattggt cctttttttg aaggtggtcg tgttttagac cttttttctg gcagtggtag
     7741 cttagctatt gaggcgatct caaggggaat ggaccaagct gtcctagttg aaaaagatag
     7801 gcgtgcgcag gtcgttattc aagaaaatat tgcaatgact aagagtccgg agcaatttca
     7861 attattaaaa atagaagcca accgtgcttt agaacaatta acgggacaat ttgatttggt
     7921 cttgttggac ccgccatatg ctaaggaaga aattgtaaag caaatccaaa tcatggatag
     7981 caagggttta ttaggcgatg atatcatgat tgtttgtgaa acggacaaat cagttgattt
     8041 acctgaagaa attgcttctt ttgggatatg gaaacagaaa atttatggca tttctaaagt
     8101 aacggtctac gttcgttagg aggctttggt atggattaca agttagtgat gattattctc
     8161 atcttagtca tattgattta tttgttgttg cgccttcact atttagaaga gttgctaaaa
     8221 cattatcact tcaaaaatga tgaaggagtt tgttcattat caagaacaaa aaatccattg
     8281 tatctattaa tggcaatgat tacttttttg ccaaatctta tcaattggat agctaacggt
     8341 tttgaggcaa tagacattgt tcgcctgatt cctgtggtag tctttttatg gttgtattat
     8401 tgtggagatg acgacgaatg acaaagaaag ctttatttac aggttcattt gatcctgtta
     8461 ctaatggaca tttagatatt attgagcgcg ccagctatct atttgatcat gtttacattg
     8521 ggttatttca taaccttgag aagcaaggtt acttttcgat tgagtgccgt aaaaaaatgt
     8581 tagaagaagc tatccgtcag tttaagaatg tttcagtttt agtggcgcaa gatagactag
     8641 ctgttgactt agcaagggaa gtgggagcaa aatattttgt tcgaggtctg cgtaatagcc
     8701 aggattttga ttatgaagcc aatttggaat tttttaataa acagttggca gatggtattg
     8761 agacagttta tttatcaacg tcgccatctt tgagtccgat atcatctagt cgcatccgtg
     8821 aattgataca ttttaaggct tctgtgaagc cttttgttcc aaagagtgtt tttagagaag
     8881 tggagaaaat gagtgaagaa taaagatcca aaaagaaaac ataaaagttt gctaggtctg
     8941 cttaaatggt ggattatagg ttttgctttt ttattgttag tattagcaag ccttgtggtg
     9001 aggctacctt attatttgga aatgccaggt ggagcttatg atatccgttc ggtattgaaa
     9061 gtgaataaaa aggctgataa agctaagggt tcgtacaatt ttgtagcggt atctgtcagt
     9121 caagcaacgc cagctcaggt tctttatgct tggttaactc cttttacaga attatctagt
     9181 aaagaggaga caacaggtgg ctttagtaat gacgattatc tcagaattaa tcaattctac
     9241 atggagactt ctcaaaatga gtccatctat caagctttaa agttagctaa taagcaagta
     9301 tctcttactt ataaaggagt ttatgtgctt aacttagcta aaaattcgac atttaaagat
     9361 agattacatt tagctgatac agtcacagga gttaatggca aaagtttcaa aaattcttct
     9421 caacttatca aatatgttgc agctcttcat ctaggtgata aagtaaaggt acaatacaca
     9481 agtcaaggta aaaagaaaga atcagttggt aaagttatta aactatcaaa cggtaaaaat
     9541 ggtattggta tcggtttgac agaccataca gaagtatcat ctgatgttcc tgtggacttt
     9601 aatactgaag gtgttggagg accaagtgca ggtttaatgt ttactttagc gatttacgac
     9661 caattagtta aagaagattt acgtaaagga cgtaagattg caggcactgg tactattgag
     9721 caaaatgggc atgttggcga tattggtgga gcaggtttga aagttgtttc agcggctaaa
     9781 aaaggaatgg atattttctt tgttcctaat aatccgatcg ataaaaatgc taaaaaaggc
     9841 aaaacaaagg ttcagacgaa ttatcaagag gcaaaagcag cagctaagcg attaggtacg
     9901 aagatgaaaa ttgtaccagt tcaaaatgtt cagcaagcta ttgattattt gaaaaaaaca
     9961 aaataaactc aaagagttcg ggcgtctgct cggctttttt gttttgcgac atttaactat
    10021 caaagtgcta aaatgatagt atgacagagc ttataagaat actacattta aatgatctgc
    10081 actctcattt tgagaatttt cctaaggtaa aacgattttt tcataacaat caagcgcagc
    10141 caatagagac gatttcactt gacttggggg ataatattga taaaagccat cctttgacag
    10201 aagcaagctc gggcaaggct aatgtccaat tgatgaatga actgggtatt gagcttgcta
    10261 ctattggtaa taatgaagga gttggtttat ccaaaaaaga cttggaccaa gtctacaaag
    10321 attcagattt tacggttatt gttggtaatc taaaagataa cataatcgaa ccgagttggg
    10381 caaaacctta cattatttat gagacacagc aaggaactaa gcttgctttt ttagcttata
    10441 cttttcctta ttataaaacc tatgaaccta atggatggac gatagaagat ccaattgact
    10501 gtcttaaatg ccatttacaa ataaatgaga taaaagaggc aaattgtcgt attttgatga
    10561 gtcatttagg tattcgtttt gatacaagga ttgctcaaga attttcagaa attgacctta
    10621 ttataggtgc acatacacac catttatttg aggaagggga gctaattacc ggtacatatc
    10681 ttgcagcagc tggtaaatat ggtcgctttg ttgggagtat tgatataact tttgataatc
    10741 atacactaaa agatatttta atctcaacat atgatacaaa acaactaact ggttatcctt
    10801 cggacagtga ttgggctaag gagattgagt cagaaggtaa agaacagctt agaaaagaaa
    10861 gtttaatatc ctttccagca ccattatcat tagaagaatc gtgtcaacta gtcatggatg
    10921 cgatgctgtt ttacgcaggt gcagatgttg ctattatcaa ttctggctta attgttcagc
    10981 catttgaaaa agacttttcg aggaaaaatc tgcatgaatc attacctcat caaatgcgtc
    11041 tcgctaaatt gacagtgagt agtcaagaac ttttagagat ttatgaaact atttatcaac
    11101 aaggtcaatt tttagcgcaa caaaagattc atggtatggg ctttcgtggc aagtactttg
    11161 gagaagtttt acattcggga tttgattaca aaaatggaaa aatagtgtat aatgagaaag
    11221 atattgatgc caaagaagaa gtgattcttg taatcgttga tcagtattat tttgcatcgt
    11281 attttgagtg tttaaaaaca aaaaagtaga cttgcttttt cctgaattac tcagagatgt
    11341 ggtagcagat tatttaataa atatgacact ataaatgaaa aatagataaa taaagagtag
    11401 aagctagtcg tttctctcaa tgattttaga gaaaggagta ttatgcgaaa agaagtgaca
    11461 ccagagatgc ttaactataa taagtatcct ggcccacagt ttattcactt tgaaaatatc
    11521 gttaaaagtg atgatattga atttcaactt gttattaatg aaaaatcagc ttttgatgtg
    11581 actgtctttg gacaacgttt ttctgagatt ttattaaaat atgattttat cgttggcgat
    11641 tggggtaacg agcagttgag gctaagaggc ttttacaaag atgctagtac gattagaaaa
    11701 aatagccgga tttcacgttt agaagattat attaaagagt attgtaactt tggttgtgct
    11761 tattttgtgt tggagaatcc aaatcctaga gatattaaat ttgatgatga aagacctcat
    11821 aagcgtcgta agtcaagatc caaatcacaa tcatcaaagt cacaaactag aaataatcgt
    11881 tcccagtcaa atgccaatgc tcattttaca agtaaaaagc gtaaagacac aaaacgccgt
    11941 caagaacgtc atattaaaga agagcaagat aaggaaatga cctctgcaaa gcagcatttt
    12001 gttattcgta agaaaaaata aaaaaaggat aaactgtaat cgtttattcc accatgaaga
    12061 aagaaaatat tatgcctaaa aaaactgata cacccgacta taaaccctct atttatagct
    12121 taactcgcga tgaactaata gcttgggcta ttgaacatgg agaaaagaaa ttccgtgctt
    12181 cacaaatctg ggactggctc tataaaaagc gtgtgcaaag cttcgatgaa atgactaaca
    12241 tttccaaaga ttttattgca cttttgaatg agaattttgt tgtcaatcct ctaaaacaac
    12301 gtattgttca agaatctgct gatggaactg ttaaatacct ttttgaatta cctgatggca
    12361 tgctgattga gacggttttg atgcgtcaat attatggttt atcagtatgt gtaacgactc
    12421 aagttggttg taacattggt tgcactttct gtgcaagtgg tttaataaag aaacaacgtg
    12481 atcttaacaa tggcgaaatc actgctcaaa ttatgcttgt tcaaaaatat tttgatgaac
    12541 gtggacaagg tgagcgcgtt agtcacattg tggtcatggg aataggtgaa ccctttgata
    12601 actataccaa tgtattaaaa ttcttgcgta cagttaatga tgataatgga ttagctattg
    12661 gtgcacgtca cattacagtg tctacttcag gtttagcaca taaaattcgt gaatttgcaa
    12721 atgaaggtgt acaagttaac ttagcagtat ctcttcatgc tccaaataat gaattacgat
    12781 caagtatcat gcgtattaat cgttctttcc ctcttgaaaa gttgtttgcg gctattgaat
    12841 attatattga gacgactaac cgtcgtgtaa catttgagta tattatgtta aatggtgtca
    12901 acgatacccc agaaaatgct caagagttag cagatttaac gaaaaagatt cgcaaattat
    12961 cttatgtcaa tttaattcct tacaatcctg tctcagaaca tgaccagtat agtcgtagtc
    13021 ctaaagagcg tgtggaagct ttctatgacg ttttgaagaa aaatggagtt aattgtgttg
    13081 ttcgtcaaga acatggtacg gacattgatg cagcttgcgg acaattacgt tcaaacacaa
    13141 tgaaacgcga tcgtcaaaaa gcgaaggtag ggcggtaatg cttaaaagat tatttactga
    13201 agatggggaa ttgacaaaga ttagtcgtcg tttcgtttgg atgttagtgg ttatctattg
    13261 tcttattatt gtcaggatgt gttttgggcc tcaaattatg attgaggggg tatcaactcc
    13321 gaatgttcag cgcttcggaa gaattgtagc tcttttagta ccatttaatt cttttcgtag
    13381 tttagatcag ctaactagct ttaaagagat tttttgggtt attggtcaaa atgtagtgaa
    13441 tattttactg ctgtttcctc tcattatagg gttactatcc ctaaagccaa gtttacggaa
    13501 atataaaagc gttatattac ttgctttctt gatgtctctt ttcatagagt gtactcaagt
    13561 tgttttagat attttaatag atgctaatcg ggtttttgaa atcgacgatc tatggacaaa
    13621 taccttaggc ggtcctttcg ccctatggag ttatcgaaac ataaaaggtt ggcttctaac
    13681 tattagaaaa tgaaaataat gtaaaggcta tgatattgtt caagcctttt actatttaaa
    13741 aaggatcact ttagttgacg aaagtaagct tatagaggca taatagagag ttgttaaatg
    13801 agaattttaa aacacttttt atgtcttctg attgatttgg tcttctgact taattatgat
    13861 gtttttcaac ctttgagaaa ggaaaaatat gtctattata aaaaatttgt ggtggttttt
    13921 taaggaagaa aagaagcgct atcttattgg gattttgtca ttaagtttgg ttgcagtgtt
    13981 aaatcttatc cctcctaaaa tcatgggatc agttattgat gctattacaa ctggaaaatt
    14041 aacaagacca caattactat ggaatttatt aggtttggtt ttgtcagctt tagctatgta
    14101 tgggctgcgt tatatttggc gtatgtatat tttagggact tcttacaaat taggccaagt
    14161 tgtcagatac cgtttatttg aacattttac aaaaatgtct ccttcttttt atcagaaata
    14221 tcgtacaggt gatttaatgg cgcacgcgac caacgacatc aattctctaa cacgtcttgc
    14281 aggaggagga gttatgtcag cagtggatgc ctctatcaca gcattagtaa cgcttatcac
    14341 catgttcttt actatttcgt ggcaaatgac attaattgcg gttatccctt tgcccttaat
    14401 ggccttagca actagtaaat tggggcgaaa aacccatgaa accttcaaag aatctcaggc
    14461 agccttttca gaattaaata ataaagtgca agaaagtgtc tctggcgtca aagtgactaa
    14521 atcatttggt tatcaagaac aagaaattgc ttcttttcaa gagattaatc agatgacttt
    14581 cgtgaagaac atgcggacca tgacttatga tgtcatgttt gatcctttag ttcttctttt
    14641 tataggtgcc tcctacgtat taacattggc tatgggagct tttatgattt caaaaggtca
    14701 agttactgtt ggtgacttgg taacatttgt gacgtattta gatatgttgg tatggccctt
    14761 gatggcgatt ggtttcttgt tcaatatggt acagcgtggt agtgtttctt ataaccgtat
    14821 taatagtcta cttgagcaag aatcggatat aactgatcct ttaaatccta tcaaacctgt
    14881 tgtcaatgga acattaagat atgatattga tttttttaga tacgacaatg aggaaacctt
    14941 agccgatatt catttcacct tagaaaaagg tcaaacctta ggtttggtag gtcaaacggg
    15001 atcagggaag acaagtctta ttaagttatt gctacgtgaa catgatgtga ctcaggggaa
    15061 aattacttta aataaacatg atatacgtga ttatcgattg tctgagttac gtcaactaat
    15121 cggttatgtt cctcaagatc agtttttatt tgctaccagt attttagaaa atgttcgctt
    15181 tggaaatcca actctatcta tcaatgctgt caaagaagca actaaattgg cacatgttta
    15241 cgatgacatt gaacagatgc cagcaggatt tgagactcta attggagaaa aaggagtctc
    15301 attatctggt ggacaaaaac aaaggattgc gatgagtcgt gccatgattt tagatccaga
    15361 tattcttatt ttggatgatt ctctatcagc agtggacgct aaaacggaac atgctattgt
    15421 tgagaatctt aaaacgaatc gtcaagggaa atcgactatt atttcagcac atcgtttatc
    15481 agctgttgtg cacgcagacc ttatcttagt tatgcgagac ggcagagtca ttgagcgagg
    15541 tcaacatcaa gagttgctaa ataaaggtgg ttggtatgct gaaacgtatg cctcacagca
    15601 attagaaatg gaggaagcat ttgatgaagt ctaatcaatg gcaagtcttt aagagattaa
    15661 tctcctattt acgcccttat aaatggttta cagtattagc tctatctctc ttattgttga
    15721 cgactgttgt taaaaatatt attcctttaa ttgcttcaca ttttattgat cactatctga
    15781 caaatgttaa tcaaacagca gttcttattt tagtgggata ttattcaatg tatgtcttgc
    15841 agaccttaat tcaatatttt gggaatctct tttttgcgcg tgtttcttat agtattgtta
    15901 gagatattcg tagagatgct tttgctaata tggaaaggct aggcatgtct tattttgata
    15961 ggacaccggc aggatctatt gtgtcacgta ttactaatga tactgaagca atatctgata
    16021 tgttttcggg tattttatca agttttatct cggcgatatt tatttttaca gttactctgt
    16081 acactatgtt gatgctagac attaaactaa caggactcgt cgctcttttg ttacctgtta
    16141 tctttatatt agtgaatgtc tatcggaaaa aatcagtcac tgtcattgct aaaacgagaa
    16201 gtttacttag tgatatcaac agtaaattat cagaaagtat tgaaggaatt cgcattgtac
    16261 aggcttttgg tcaagaagag cgcttgaaga ctgaatttga ggaaattaac aaagagcatg
    16321 ttgtgtatgc caatcgttct atggctcttg atagtctctt cttaagaccg gcgatgtctc
    16381 ttttaaaact cctagcatat gctgttctta tgtcttattt tggatttaca ggagttaaag
    16441 gaggtcttac ggcaggatta atgtatgctt ttattcagta cgttaatcgt ctatttgacc
    16501 ctttaattga agtaacgcaa aatttttcaa ccttacaaac atcaatggta tcagcagggc
    16561 gtgtgtttga tctgattgat gaaacaggtt ttgaaccaag ccaaaaaaat acagaagctt
    16621 ttgtgagaga aggaaatatc gaatttaaaa atgtatcatt ctcatatgat ggaaaaaaac
    16681 aaatccttga taatgtttct tttagtgtta aaaaaggtga aactattgct tttgtaggag
    16741 cgacaggttc tggaaaatca tctattatta atgtctttat gaggttttac gaatttcaat
    16801 ctggacaagt tttattagat gggaaggata ttagggatta ctcacaagaa caattgcgaa
    16861 agaatattgg actcgtctta caagacccct ttttatatca tggcactatt aaatctaata
    16921 tcaaaatgta ccaagacatc accgatcaag aggtacagga cgcagcagag tttgttgatg
    16981 ctgatcaatt tattcagaag ttgccagata agtacgattc agctgtttca gaacgtgggt
    17041 caagtttttc aactggacaa cgccaactat tagcgtttgc cagaactgtg gctagtaaac
    17101 ctaaaatttt aatcttagat gaggcgacag caaatattga ttcggaaaca gagcagattg
    17161 tacaagattc gttagcaaaa atgcgtcaag gtcgaacaac aattgccatt gctcaccgct
    17221 tatcaaccat tcaagatgct aattgtatct atgttcttga tagaggaaag ataattgaaa
    17281 gtgggaatca cgagtcttta ttagaccaaa aagggactta ttatcgtatg taccaattac
    17341 aagccggcat gatggaggtc tagaaaattt aggctattca aaaggcataa tctgtgaggc
    17401 ttagaggttg gaatagttat tcctctaagc cttttttata ttttacaatt agtgtataat
    17461 gaggtcaaag tacaaggaaa ggtctataaa atgttactat tggtagataa ttacgattct
    17521 tttacctata atttaaagca atatttaagt gtctataaag aaatattcgt cataaaaaat
    17581 gatgatccta accttttttt attagctgag agcgcagaag ctatcgtctt atcacctgga
    17641 ccaggacacc caaaggatgc tgggaaaata ttagaattaa tcaatcagtt tataggacag
    17701 aaacctattc taggtatctg tttggggcat caagcgcttg ctgagtgctt gggtgggcga
    17761 ttaaatttag caaatcacgt tatgcatggt aaacaaagtt gggtaacaat aaacgaccat
    17821 actagtttgt ttaagggaat tgatagtcca acccaagtta tgcgttatca ctcattggta
    17881 gtgactgatt taccagagaa tattgctatt attgcacgat caaatgagga taatgaaatc
    17941 atggcattcc attgccccag cttaaaggta tatgcgatgc agtttcatcc tgagtcaatc
    18001 gggagtattg atggaatgaa aatgattgaa aactttttga cgttaattaa tgactaaaat
    18061 gtgttagtgt aggataatga ctctctataa ataaaagaca gtacctaagg gcactgtctt
    18121 ttatttagtt gtatagatga gttaatctgt ctgcaaattt acgataaata atcaagcaaa
    18181 tggatgcttt taaggtatcc ccaggaataa aaataaggtt agaaaatagt gatttagtta
    18241 aggggagaga agtttgaaag gaaagaacga tgctaccaca aatatcaatc aatagtacgc
    18301 caaatatcca aataataagg aattaaaccc agagttttgt tgttttcact ttctcaagcc
    18361 ctaggccgat aaaaaaagct gcaaatggat aagtcagcag atagcctgct gttggcccaa
    18421 acagcgttac gagacctgac cgccctcctg gtaaaatggt gcaccaatag caactaagag
    18481 caagaaaata gccacagcta ggaaaccttt cctactacca agcagagcac ctgccagcat
    18541 aacaccaaga ttttgtaaga caattgggac tggaatgaaa cctaatggga tccctgggat
    18601 aaaacctaaa acaatgagta gtgcaaccat gagtgcaata taggtagttg ttcttgtttt
    18661 catttaaatt aattcctttt tagtatgatt tagttgtaat ttttccaaga attcaatatc
    18721 ggtatgttga ttgcgacccc cctctgttag gtaatttccc aaaaaagttg agtcaacaag
    18781 taaggtgact aatgattcaa aattctcaaa atggacctcc cgtccagcac ttaatcttaa
    18841 ttccttgaaa ggaaaaacaa aacgcaacat ggccataatt tttaagcatt taatgggagt
    18901 caaatagtta tattttccaa gaggtgttcc ttcaacagca agtaaaaaat tgacagggat
    18961 agaatagggg ttcagttctt ttagtctgaa agctaatgtg atgagcccct catcgctctc
    19021 tcccatacca caaatgaatc cagaacaaac atcaatgtct tcattgtgaa ttctttctaa
    19081 agtatcacaa cgatctttga agctatgcgt cgttgcaatg ttggggtagt tttcttcggg
    19141 ggtgttgagg ttatgattga tacggtcaat tcctgcttgt tttagctgat gaagttgttc
    19201 tctatccaaa aaaccagcgg taaggcatat ttctagagga aggctttttt tgatttcagg
    19261 gatgacttga cataactgct caatttctct cttgctaggc ttagttccac tcatcgcaat
    19321 acaaaagacg ctagcaccat tttgatgagc gacaatagcc tgttttagaa tgacattttg
    19381 aggaagcaga ccaaatttat caatgtcagc agtgctttct ttagattgag aacagtaacc
    19441 acaattttct gcacaaagtc cttgcttagc acttaataaa acgttaaggc gaatattatt
    19501 tcggctaact tgatttttaa ggtataaggc agcagcgtag atttcccacc agttctcatc
    19561 gctatttaga atttccagcg cttgttcata agaaatgcta gtctttccag ataatatttc
    19621 gtcagccaaa tggatataat ttgtttgaaa tgacatgata gcctccttta acgaaattaa
    19681 taatagcata aaaaaactgt attgtaaact tagtctatat aataagttaa caatataaaa
    19741 aattatgata tactggtctt atattttgac gggagataag atgaaaaaac tatttaaaaa
    19801 agcgatagct atgtgccgag atttgccgct taattttgta attgttgaac ttagtttgta
    19861 tcaaagagag gatggctcat ctgataatat taaatggatt tctaagcgaa tccttgaggt
    19921 aacggtaact gatcagcaat ggatttacca tttgaggatg cttaatttac caaaacttaa
    19981 gccaatgtca ctagcatcca gtgactgtcc tgcaagggaa gagaaggcta gtcttatttc
    20041 aatagaagaa tgtcaaaaat tcactagctt agtgcgagat aaaaatcctt ttcaccaagg
    20101 ggaagcagct attgtgcctg cggctctatt attggaaaaa ataatggaag ttagaaatgg
    20161 aaaaataaag gagattcatt tgactttttt atctccagta agggtgaatc aagaattctc
    20221 agtgcaagta gtggataaca cagtgattat ttttcagcat caaatattat gtgtgagggg
    20281 gacatatgaa gaatagagat gtttatattg gttttgggct acgaacacct atcggtataa
    20341 aagggaagca atttaaacat taccgtccag aacttttagg agcacacctc ttaaatcaaa
    20401 taaaaaaaat agaatcagaa tctaatattg atagtattat ttgtgggaac acagttggta
    20461 ctgggggcaa tattggtcgt ttgatgactc ttttttctga ttatgaatcc tatattccag
    20521 tacaaacgat tgatatgcag tgtgcttcat caagttctgc cttgtttttt ggttatctaa
    20581 aaatcagtgc cggtattaat gaaaaagttc ttgttggggg gattgaaagt agttctcttc
    20641 aacctatgag acgttacgct aaagaagata atcgtaacgg agaatatacc gttgctcagt
    20701 tttctcctga ctcttatgct gaaactgtaa tgttagaagg ggcacaaaga gtctgtcaaa
    20761 aatatggttt tagaagagaa atgttagata aattggcatt cttgagccat aaacgcgcct
    20821 taacagctaa acaaggtggc tatttagaag aggtaatctt accaatggaa gggatgcgag
    20881 atcaaggcgt tagaaaacta aaagaagcat tttttcaaaa attaccaaga ttgatggaaa
    20941 attcaccttt gctcactatt ggaaatgttt gtttaatgca cgatgctgct gcatttctaa
    21001 cgcttcagag tcagaaaaca gaattccgga ttgttcacat tgtagaagta gcaggagatc
    21061 ccaagcttag tccagaattg gttcacacgg ctacggaaaa actattaaca gaaactcata
    21121 ctaaaatatc ggattatgat gcaattgaat ggaatgaacc atttgcagcg attgatgctt
    21181 tatttaatca ttattatcct gaagagagag aaaaattcaa tatttttgga ggggcattag
    21241 cttacggaca cccttatgcc tgctcaggaa ttattaatat ccttcatctt atgcaggcat
    21301 taaaatataa aaataaacct atgggtctaa ctgccattgc aggggcagga ggagtcggaa
    21361 tggccatatc aatagagtac ttaggagtta agaatgcttg aatcattgaa aacaatagtt
    21421 aaaaccaata gcgataaaaa gttatttgat ggtgatttac aggtcagtta tggagaattt
    21481 tataacttag tcagacaaga tatggcgtca caagacaata ggaaacatgt gattagcaca
    21541 cactctctgc ttaaccaatt agtaagattt gtttcaaagt tatgtcaaaa agctcttcct
    21601 attatttgta aacctaacct tactcataat gaaataagtc gattagaaaa agaagtgcag
    21661 tatgctcctc aactagctga ttttggtgtt ctttcttctg ggactacagc tgatgctaaa
    21721 ctattgtgga ggagcttcgc ttcgtggtct gattttttta gcattcagaa tgcttatttt
    21781 tcggtcactt ccaatagcag actgtttatt caaggtgatt tttcatttac tggtaactta
    21841 aatttagcgc tatccctttt attactgggg ggaacgcttg ttgtcacaca gaaaaattct
    21901 gtaaaatact ggcagacttt atgggaaaaa actggtgtaa cgcatctata tctgctccct
    21961 agttatctca agttagtgga acaatatagc aaggagactg cgttagacaa taaaactatt
    22021 attacctctt cgcaatatgt ctctgactca ttactggaag gtttatatat aaagcatcct
    22081 aaagtgtcag tgaaaatctt ctatggtgct agtgaactca attatgtgtc atggtacgat
    22141 ggtcgtgata tccgagataa gccacagtat gttggagaaa tcgttccaaa tgtagctgtt
    22201 aggattaaag agggaagaat ttttgtaaaa acaccgtata gtatctgtgg cctttcaagt
    22261 gaatattgtg ctggtgatta tggtgaactg atagatggca aattatatct atttggtcgt
    22321 ggtgacgatt ggtgcaatca atcgggcatt aagctctatt tacctagatt aattgaaaaa
    22381 ataaaaacat gcccttatat caaggatgca gtagcattta caaaagagag tcaaagtcat
    22441 ggacaagagt cacattgctg tattgtttta attgaaaatc agatgcaaca agagtgtttg
    22501 aagtggttat cggaacattt tgaaaagaag tatgggttta agcattatca tattgtatct
    22561 aaaattcctt tgatgccatc tggaaaaatt gattatcaac aactcaaaag acaattagca
    22621 tgagttttta ctaaaaaaat aaaatagggt taatggattg caaacctcag ttctgatatt
    22681 ctttattttg aaagatatcg tgatataatg ataaaaaaga aagataagga ataaaatgtt
    22741 atcaaaagca aaatcacgat atattatccg tgagattatt aagttatttc cagatgctaa
    22801 acctagctta gactttacta atgtctttga gcttttggta gctgtaatgc tatcagctca
    22861 aacaactgat gcagctgtta ataaagtcac tcccgccctt tttgaacgct ttccaaatcc
    22921 tttagttttg gcacaagctg atcccaaaga gattgaaccc tatatttcta aaattgggct
    22981 ttaccgtaat aaagctcgat tcttaaatca atgcgctaaa caattgatag agcattttga
    23041 tgggaaagtc ccccaaactc gtcaagagct agaaagtttg tctggcgttg gaaggaaaac
    23101 ggctaatgtg gtcatgagtg ttggatttgg tattccggct tttgccgtag atactcatgt
    23161 cactcggatt tgtaaacacc accagatttg caaacagtcg gcttctccac tagaaattga
    23221 gaaacgagtc atggaggttt taccgccaga agagtggcta gctgctcatc aatccatgat
    23281 ttattttggt cgtgcaattt gtcatcctaa aaatccaaaa tgcgaccagt acccacaact
    23341 ttatcacttt cctgataatt taaagtgatt agaataaaag agagtaactg gtgacgatta
    23401 tataagcgat acttaaaaac ggtagaaaag ctatcgtttt tttcattttt ctcataacta
    23461 aacaataaat aattcctaac caagcaccta tttgaataat aaaaagcatt tgatgaagag
    23521 gtaaagataa gccaattgtg gctaaataaa ggaaatctcc acttccgata ttaatatcaa
    23581 ccacttcagc taaaatccca aataaacata aaacaataga aacttttgta atcggtgtga
    23641 gaattatcgt tgaaatggtg aataatatcc atag
//
LOCUS       DATROQ010000026        23131 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.026, whole genome shotgun sequence.
ACCESSION   DATROQ010000026 DATROQ010000000
VERSION     DATROQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23131
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.026"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            5..1339
                     /locus_tag="VBC94_000892"
     CDS             5..1339
                     /locus_tag="VBC94_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEO6979171.1"
                     /translation="MEDPGSQSLLLQFVILLILTLFNAFFSASEMALVSLNRSKVEQK
                     AEEGDKRYRRLLDVLENPNNFLSTIQVGITFISLLQGASLSASLGHVISGWLGNSATA
                     RTAGSIIALIFLTYVSIVLGELYPKRIAMNLKDRLAIVSAPIIIFLGKIVSPFVWLLS
                     ASTNLLSRITPMTFDDADEKMTRDEIEYMLTNSEETLDAEEIEMLQGIFSLDEMMARE
                     VMVPRTDAFMIDINNDAQSNIEGILSQNFSRVPVYDDDKDRVVGVLHTKRLLEAGFKT
                     GFDTIDLRKILQEPLFVPETIFVDDLLKALRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDTAEQFVREIDENIYIVLGTMTLNEFNDYFETELESDDVDTIAGYYLT
                     GVGSIPNQEEKVAYEVDSKDKRITLINDKVKDGRITKLKVLLSDIEQNIEKD"
     gene            1407..2195
                     /gene="pflA"
                     /locus_tag="VBC94_000893"
     CDS             1407..2195
                     /gene="pflA"
                     /locus_tag="VBC94_000893"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEO6979172.1"
                     /translation="MAEIDYKKVTGMIHSTESFGSVDGPGIRFIIFMQGCKMRCQYCH
                     NPDTWEMETNNSKERTVEDVLKEALRYKHFWGKDGGITVSGGEAMLRIDFITALFIEA
                     KKLGIHTTLDTCGFAYRATPEYHAILEKLLDVTDLVLLDLKEIDPEQHKIVTRQSNKN
                     ILQFARYLSDRGTPVWIRHVLVPGLTDIDDHLKRLGEFVQTLDNVDKFEVLPYHTMGE
                     FKWRELGIPYPLAGVKPPTPERVKNAKDIMKTESYTEYLKRIQN"
     gene            2312..3247
                     /locus_tag="VBC94_000894"
     CDS             2312..3247
                     /locus_tag="VBC94_000894"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEO6979173.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAWGLDTEAVALGTPN
                     EETAYVLDYFGVQAPRVVESAKAEGVETVILTDHNEFQQSISDIKDVTVYGVVDHHRV
                     ANFETANPLYMRLEPVGSASSIVYRMFKENGVSVPKELAGLLLSGLISDTLLLKSPTT
                     HASDIPVAKELAELAGVNLEEYGLEMLKAGTNLSSKTAAELIDIDAKTFELNGEAVRV
                     AQVNTVDINDILARQEEIEVAIQEAIVTEGYSDFVLMITDIVNSNSEILALGSNMAKV
                     EAAFEFTLENNHAFLAGAVSRKKQVVPQLTESYNA"
     gene            3307..3960
                     /locus_tag="VBC94_000895"
     CDS             3307..3960
                     /locus_tag="VBC94_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEO6979174.1"
                     /translation="MILTFNPGKLERQEFFKELINYLWIHDDVTLRQIKSHFTDYSKI
                     DRLLEEYINHGYILRQNKRYSLNLPFLSSLDGLTLDDLVFIDSASQIYQLLQKRKFVT
                     NLDNQTNHLVFVEETDFERNTLTLSNYFYKLTNCYPLSREQKKLYQLLGDVNSEYALK
                     YMSSFILKFLRKDSVKQKRTDIFIQALELLGYISLNQDTTYRLNAKLDVEALKIYLT"
     gene            complement(3990..4991)
                     /locus_tag="VBC94_000896"
     CDS             complement(3990..4991)
                     /locus_tag="VBC94_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO6979175.1"
                     /translation="MIQKNKAPFVLISSVIILLLLILVSISLGYANTSVIDVLKLISG
                     KSDDAFLFIITNIRLPRIIVCIFGGASLGIAGLLLQTLTKNPLADSGILGINAGAGLV
                     IALTIGTFNVSNPTILYFLPLFAMFGGLVTIFLVYLMSYRRNHNISPTRLIITGIGIS
                     TMISGVMISIISQSNNQKMDMIVEWLSGKITISSWTTIITFIPILILLWGLAYSRSRH
                     LNIMNLNEQTALALGLHLKKERIYTLMLASSLAAISVVLIGNITFIGLLAGHLSRRLL
                     GNNHKIILPSCLLIGAIILLVSDTIGRLLLVGTGIPTGLVVSIIGAPYFLWLMTKVD"
     gene            complement(4988..6013)
                     /locus_tag="VBC94_000897"
     CDS             complement(4988..6013)
                     /locus_tag="VBC94_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO6979176.1"
                     /translation="MFKASKKLVQKNKSNHFWLVFFITLILFLIGCYASLRFGAINFK
                     TSDLITVLKNPLKNSNAQDVIFDIRLPRIIAAILVGAAMSQAGAIMQGVTRNAIADPG
                     LLGINAGAGLALVVAYAFLGSMHYSTILIVCLLGSVISCLLVFTLSYTKQKGYHQLRL
                     ILAGAMISTLFTSVGQVVTLYFKLNRTVIGWQAGGLSQINWKMLIIIAPIIILGLLIS
                     QLLAHQLTILSLNESVAKALGQKTQLMTAFLLLIVLFLSASSVALIGTVSFIGLIIPH
                     FIKLFIPKDYRLLLPLIGFSGATFMIWVDLSSRIINPPSETPISSIISIVGLPCFLWL
                     IRKGKNL"
     gene            complement(6029..6961)
                     /locus_tag="VBC94_000898"
     CDS             complement(6029..6961)
                     /locus_tag="VBC94_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6979177.1"
                     /translation="MKKIGIIVLTLLTFFLVSCRQQTKEESTKTTISKMPKIEGFTYY
                     GKIPENPKKVINFTYSYTGYLLKLGVNVSSYSLDLEKDSPVFGKQLKEAKKLTADDTE
                     AIAAQKPDLIMVFDQDPNINTLKKIAPTLVIKYGAQNYLDMMPALGKVFGKEKEANQW
                     VSQWKTKTLAAKKDLHHILKPNTTFTIMDFYDKNIYLYGNNFGRGGELIYDSLGYAAP
                     EKVKKDVFKKGWFTVSQEAIGDYVGDYALVNINKTTKKAASSLKESDVWKNLPAVKKG
                     HIIESNYDVFYFSDPLSLEAQLKSFTKAIKENTN"
     gene            complement(6985..7779)
                     /locus_tag="VBC94_000899"
     CDS             complement(6985..7779)
                     /locus_tag="VBC94_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979178.1"
                     /translation="MSHIKAENIIVSYDQKEIINNLSLSILNQKITTIIGANGCGKST
                     LLKALTRIHKIKDGTITIDGHDIAHLPTKEIAKKIALLPQVLEATEGITVYELISYGR
                     FPHQKYLGNLTNDDRSKIHWAMEMTNVAQFANRDVDDLSGGQRQKVWIAMALAQDTDT
                     IFLDEPTTYLDMNHQLEVLELLKKLNNETQKTIIMVLHDLNLSARYSDYLVAMKTGKI
                     IYEGSPSQIMTKDIIKDIFKIDAHIIQDPISKQPVLLSYQLSTDQN"
     gene            complement(7938..9389)
                     /locus_tag="VBC94_000900"
     CDS             complement(7938..9389)
                     /locus_tag="VBC94_000900"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEO6979179.1"
                     /translation="MITIDKILEILKNDHNFREILFHEHYYYNWTQNVTFNALSYDSR
                     QISSDTLFFAKGATFKKEYLDSAITAGLSFYVSEIDYGADIPVILVNDIKKAMSLISM
                     SFYNNPQNKLKLLAFTGTKGKTTAAYFAYHMLKVNHRPAMLSTMNTTLDGKSFFKSHL
                     TTPESLDLFRMMATAVENQMTHLVMEVSSQAYLTKRVYGLTFDVGVFLNISPDHIGPI
                     EHPTFEDYFFHKRLLMENSNAVVVNSQMDHFNIVKEQVEYIPHDFYGDYSENVITESQ
                     AFSFHVKGKLENTYNINLIGKFNQENALAAGLACLRLGVSIEDIKNGIAQTTVPGRME
                     VLTQTNGAKIFVDYAHNGDSLKKLLAVVEEHQKGDIILVLGAPGNKGQSRRKDFGDVI
                     NQHPNLQVILTADDPNFEDPLVISQEIASHINRPVTIIIDREEAIANASTLTNCKLDA
                     IIIAGKGADAYQIIKGNRDNYSGDLEVAKKYLK"
     gene            9513..11147
                     /locus_tag="VBC94_000901"
     CDS             9513..11147
                     /locus_tag="VBC94_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO6979180.1"
                     /translation="MSQKTTKVSQQEQMVKGTAWLTAGNFISRLLGAIYIIPWYVWMG
                     KHAAEANALFGMGYEIYALFLLISTVGIPVAVAKQVSKYNTLGKEEMSIYLVRKILQF
                     MLILGGVFALIMYIGSPLFASLSKGGQELVPILRSLTLAVLVFPSMSVLRGFFQGFNN
                     LKPYAISQVAEQIIRVIWMLLTAFYIMRLGSGDYIAAVTQSTFAAFVGMFASIAVLLY
                     FLWRYNMLSALISKTPEHIKLDTKEILIETVKEAIPFIITGAAIQIFKLIDQFSFGNT
                     MALFTNYSSEELRVMFAYFSSNPGKVTMILIAVATAIAGVGIPLLTENFVKNDKKAAA
                     RLVVNNLQMLLMFLLPAVAGSVILAKPLYTVFYGLPQGQALGLFVISLIQTIILSIYT
                     VLAPMLQALFENRKAIIYFLYGLVAKVILQVPSIFLFHAYGPLFSTTVALCIPVILMY
                     LKIHEITGFKRQAIRRTSALVLILTLLMSFIISMIIWLMNLVIVPDSRLVSLVYIIVI
                     GAIGLGVYGFMALATHLLDKMIGSRAQDLRRKLHLN"
     gene            11282..12502
                     /gene="pepT"
                     /locus_tag="VBC94_000902"
     CDS             11282..12502
                     /gene="pepT"
                     /locus_tag="VBC94_000902"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEO6979181.1"
                     /translation="MSYEKLLERFLTYVKINTRSNSNSTQTPTTQSQVDFALTVLKPE
                     MEAIGLKDVHYLPSNGYLVGTLPATSDRLRHKIGFISHMDTADFNAENITPQIVDYKG
                     GDIELGDSGYILSPKDFPNLNNYHGQTLITTDGKTLLGADDKSGIAEIMTAMEYLASH
                     PEIEHCEIRVGFGPDEEIGIGADKFDVKDFDVDFAYTVDGGPLGELQYETFSAAGLEL
                     TFEGRNVHPGTAKNQMINALQLAIDFHSQLPENERPEQTDGYQGFYHLYDLSGTVDQA
                     KSSYIIRDFEEVNFLKRKQLAQAIADNMNEALQSERVKVKLYDQYYNMKKVIEKDMTP
                     INIAKEVMEELDIKPIIEPIRGGTDGSKISFMGIPTPNLFAGGENMHGRFEFVSLQTM
                     EKAVDVILGIVAKD"
     gene            12531..13022
                     /locus_tag="VBC94_000903"
     CDS             12531..13022
                     /locus_tag="VBC94_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEO6979182.1"
                     /translation="MIKLFGKIRYHWQPELSWAIIYWSIAIAPIFIGLSLLYERTEIP
                     SQVFVLFAIFIVLVGIGFHRYFVIEEDGYLRIVSFNFLRRSKFPIEDIAKIEVTKSSV
                     TIKFNNNHERIFYMRKWPKKYFLDALAIEPKFKGEVELLDNLIKMDYFECYRYDKKAL
                     TKS"
     gene            complement(13009..13206)
                     /locus_tag="VBC94_000904"
     CDS             complement(13009..13206)
                     /locus_tag="VBC94_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEO6979183.1"
                     /translation="MKVALIPEKCIACGLCQTYSNIFDYQDDGIVKFSDTDNLEKEIP
                     SSDQDTVLAVKSCPTKALTIL"
     gene            13252..13776
                     /locus_tag="VBC94_000905"
     CDS             13252..13776
                     /locus_tag="VBC94_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO6979184.1"
                     /translation="MAKKPWEKKVVENNSHRKDKITRTSRGVVSSTPWITAFLSAFFV
                     IVVAILFIVFYTSNRGEDRAKETSGFYGASSQKVNSSKTKKASTSKKTTSSSKTKTSD
                     KSTTSSSTVASSSETSTSSSVNGGTIVVQPGEGAASIAARAGISVARLQELNPGHMTS
                     GYWYANPGDPVVVN"
     gene            13787..14470
                     /locus_tag="VBC94_000906"
     CDS             13787..14470
                     /locus_tag="VBC94_000906"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEO6979185.1"
                     /translation="MNSINIAIDGPASSGKSTVAKIIAKNLNYTYLDTGAMYRCATYL
                     ALQHGYEAQDVSKILGLLAERPISFGKAEDGSQTVFLGTEEVTLAIRQNDVTNNVSWV
                     SAIPEIREELVNQQRRIAKDGAIIMDGRDIGTVVLPDAELKIFLVASVDERAERRFKE
                     NQEKGIESDFETLKSEIAARDYKDSHREVSPLKAAEDAIEFDTTGVSIEGVVTFIQEK
                     AEKIIDMKN"
     misc_feature    14491..14616
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14631..15161
                     /gene="infC"
                     /locus_tag="VBC94_000907"
     CDS             14631..15161
                     /gene="infC"
                     /locus_tag="VBC94_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEO6979186.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQAIADD
                     ANVDLVLIQPQATPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPIPDKK"
     gene            15201..15401
                     /gene="rpmI"
                     /locus_tag="VBC94_000908"
     CDS             15201..15401
                     /gene="rpmI"
                     /locus_tag="VBC94_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEO6979187.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFHAFTSHRFHGKTKKQRRHL
                     RKATMVSSGDFKRIKAMLTRLK"
     gene            15459..15818
                     /gene="rplT"
                     /locus_tag="VBC94_000909"
     CDS             15459..15818
                     /gene="rplT"
                     /locus_tag="VBC94_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEO6979188.1"
                     /translation="MARVKGGVVSRKRRKRVLKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AAAFTALADAAKAKLGK"
     gene            complement(16061..18205)
                     /locus_tag="VBC94_000910"
     CDS             complement(16061..18205)
                     /locus_tag="VBC94_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEO6979189.1"
                     /translation="MKKIKDFASRAINTRLGFILLLVVIYWLKTIWAYHTDFNLGLEN
                     SYQLFLTIINPIPLGLLIIGLALYVKRTKAFYITAFITYAIVNILLIANAIYYREFSD
                     FITVSAVLASSKTSAGLGDSALNLLRIWDLVYVFDFIILIFLFATKKIHLDNRPFNKR
                     ASFSITALSGLLFSINLFLAEIDRPELLSRGFSNTYIVKALGLPSFSIYSGNQTYQAQ
                     KERNGATAQELATAKKYVAEHYAKPNPEYYGIGKGRNVIMIHLESFQQFLIDYKLNVD
                     GKEHVVTPFINSLYHSKETISFSNFFHQVKAGKTSDAETLMENSLFGLSSGSFMVNYG
                     GENTQFAAPHILAQNGGYSSAVFHGNVGTFWNRNNAYKQWGYDYFFDSSYFSKQTKDN
                     SFQYGLNDKYMFADSIKYLEHMQQPFYTKFITVSNHYPYTSLKGESDEEGFPLAKTND
                     ETINGYFATANYLDTALKSFFEYLKAAGVYDNSIIVMYGDHYGISNTRNPSLAELLGK
                     DPETWSEYDNAMLQRVPYMIHIPGYSKGFISNTYGGEVDNLPTLLHILGIDTSKYTQL
                     GQDLLSKDNKQMVAMRTTGQYITPKYTNYSGHLYYTDSGQEITNPDETTKAEIKTIRD
                     ATNKQLSTSDSIQTGDLLRFDENNGLKTVEVEKFNYTHSLKALKAKERKLKDRSTSIY
                     SKHNNKSTVDLFHAPSYLELQDPNKTHKTSKK"
     gene            18340..19497
                     /locus_tag="VBC94_000911"
     CDS             18340..19497
                     /locus_tag="VBC94_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEO6979190.1"
                     /translation="MNKLKVNSVVERKIKSGAQLLEKKDFDTSLVNQLVQLFSQSNQF
                     LGMAYLSPQNKGIGWLLSRQIFDFNHDYFVSLFEKSREKRQKFEKSSQTTAYRLFNQD
                     GDNFGGLTIDFYSDYALFSWYNEFVYTNRQMIVAAFKQVYPNIKGAYEKIRFKGLDFE
                     SAHLYGQEAPESFLILENNIKYSVFLNDGLMTGIFLDQHDVRKALATNLSEGKKVLNM
                     FSYTAAFSVAAAVGGALETTSVDLAKRSRELSKAHFDANQIVTDNHRFIVMDVFEYYK
                     YAKRKHLSYDVIVIDPPSFARNKKQTFSVTKDYYKLIEQALDILAPGGTIIASTNAAN
                     LTVSQFKKQLEKGFGKASHNYISLQQLPEDFTVNDKDQQSNYLKVFTIKVK"
     gene            19497..20174
                     /locus_tag="VBC94_000912"
     CDS             19497..20174
                     /locus_tag="VBC94_000912"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEO6979191.1"
                     /translation="MKIVVPVMPRSLEEAQEIDLSKFDSVDIIEWRADALPKDDIINV
                     APAIFEKFAGHEIIFTLRTTREGGNIVLSDAEYVELIQKINSIYNPDYIDFEYFSHKE
                     VFQEMLEFPNLVLSYHNFQETPENIMEIFSELTALAPRVVKIAVMPKNEQDVLDVMNY
                     TRGFKTINPDQVYATVSMSKIGRISRFAGDVTGSSWTFAYLDSSIAPGQITISEMKRV
                     KALLDAD"
     gene            20268..21335
                     /locus_tag="VBC94_000913"
     CDS             20268..21335
                     /locus_tag="VBC94_000913"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEO6979192.1"
                     /translation="MQVEVDLPNHPYHIKIEEGCFSEAGDWVSHLWQKQMITIITDSN
                     VEILYGESLVNQLKKQGFTVHVFSFAAGEASKTLEVANRIYGFLAKHHMTRSDGIIAL
                     GGGVVGDLAAFVASTYMRGIHFLQIPTSLTAQVDSSIGGKTGVNTSFAKNMVGTFAQP
                     DGVLIDPVTLKTLGNRELVEGMGEVIKYGLIDDIKLWHILEEMDGSIDSILDNALAII
                     YHSCQVKRKHVLADQYDKGLRMHLNFGHTIGHAIEVHAGYGEIMHGEAVAIGMIQLSR
                     VAERKNLMPRGISQDIYNMCLKFGLPVHYAEWDKDVLFNILSHDKKASGQFIKIVILP
                     QLGSATVHQIPLEEMRDYLEK"
     gene            21336..22502
                     /gene="aroC"
                     /locus_tag="VBC94_000914"
     CDS             21336..22502
                     /gene="aroC"
                     /locus_tag="VBC94_000914"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEO6979193.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLKLSAKDINEDLKRRQGGYG
                     RGNRMKIETDQVIISSGVRHGKTLGSPITLTVTNKDHSKWLDIMSVEDIEERLKQKRR
                     IKHPRPGHADLVGGIKYRFDDLRNALERSSARETTMRVAIGAIAKRILKEIGIEIANH
                     IVVFGGKEITVPDKLTVQQIKVLSSQSQVAIVNPSFEQEIKDYIDSVKKAGDTIGGVI
                     ETIVGGVPVGLGSYVHWDRKLDAKIAQAVVSINAFKGVEFGLGFKSGFLKGSQVMDSI
                     SWTKDQGYIRQSNNLGGFEGGMTNGEPIVVRGVMKPIPTLYKPLMSVDIDTHEPYRAT
                     VERSDPTALPAAGVVMEAVVATVLVTEVLEKFSSDNMYELKEAVKLYRNYVNHF"
     gene            22586..22921
                     /locus_tag="VBC94_000915"
     CDS             22586..22921
                     /locus_tag="VBC94_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEO6979194.1"
                     /translation="MANVYDLANELERAVRALPEYQAVLTAKAAIENDADAQVLWQDF
                     LATQSKVQEMMQSGQMPSQEEQDEMSKLGEKIESNDLLKVYFDQQQRLSVYMSDIEKI
                     VFAPMQDLM"
BASE COUNT         7549 a         3641 c         4441 g         7500 t
ORIGIN      
        1 ttccatggaa gaccctggca gtcagagttt gttactacaa tttgttattt tattaatatt
       61 aaccctgttt aacgccttct tttcagcatc tgaaatggct ttagtatcgc taaaccgctc
      121 taaagtagaa cagaaagctg aagaagggga caaaaggtat cgacgtcttt tggacgttct
      181 tgaaaatccc aataattttt tatcaacaat tcaagttggt attactttta ttagcctttt
      241 gcaaggggct agtttatctg cctcactagg acatgtgatt tcaggatggc taggaaattc
      301 agcaacagcc cgtacagcag ggagtattat tgcgctaatc tttttaacat atgtctctat
      361 tgttctagga gaattatatc ctaagcgtat tgctatgaat ttgaaagatc gtctagcgat
      421 tgtttcagct cccattatta tctttctggg gaaaatagta agcccctttg tatggttgct
      481 ctcagcttca actaacttat tgagtagaat cactccgatg acttttgatg atgctgacga
      541 gaagatgaca cgtgatgaaa ttgaatatat gttgacgaat agtgaagaaa ctttggatgc
      601 tgaagaaatt gagatgctgc aagggatttt ctcgctagat gaaatgatgg cgcgtgaagt
      661 tatggttccg cgtactgatg ctttcatgat tgacatcaac aatgatgcac aatcaaatat
      721 tgaaggaatt ttatcacaaa acttttctcg tgtccctgtt tatgatgacg ataaagatag
      781 agttgttggt gttttacata ccaagcgctt attggaagcg ggctttaaaa ctggttttga
      841 tactatcgac ttgcgaaaaa tacttcagga acctcttttt gttccagaaa cgatttttgt
      901 agatgatctt ttaaaagcat tacgcaacac tcaaaatcaa atggccattt tactagatga
      961 atatggtggc gttgctggtt tagtaactct agaggattta ttggaagaaa ttgtcggcga
     1021 aattgatgat gaaacagata cagctgaaca atttgtcagg gaaatcgatg agaatatcta
     1081 tattgttcta ggaactatga cgcttaatga atttaatgat tattttgaga cagagttaga
     1141 aagtgatgat gttgatacaa ttgcgggtta ttatttaact ggtgtgggta gtattccaaa
     1201 ccaagaggaa aaagtagctt acgaagtaga cagcaaagat aaacgcatca ctttgattaa
     1261 tgataaagtt aaagatggtc gtataacaaa attgaaagtg ctattgtctg atatagaaca
     1321 gaatattgaa aaagactaag attacttagt ctttttttca tgaaaatgat ataataaagt
     1381 cataaaaacg gttacaaggt gatgaaatgg cagaaataga ctataaaaaa gtaacaggaa
     1441 tgatccattc tacagaaagt ttcggctctg tggatgggcc aggtattcgt tttatcattt
     1501 ttatgcaagg atgtaaaatg cgttgtcaat actgtcataa tcctgatact tgggaaatgg
     1561 aaactaacaa ttcaaaggaa cgcaccgttg aggatgttct aaaagaagct cttcgttata
     1621 aacatttttg gggaaaagat ggaggtatca cagtttcagg tggtgaagca atgctgcgaa
     1681 ttgactttat tacagctctc tttattgagg ctaagaaact aggtattcat acgacgctag
     1741 atacttgtgg ttttgcttat cgagcaacac cagaatacca tgctatcctt gaaaaattgt
     1801 tagatgttac tgatttggta ctccttgact taaaagaaat tgatccagaa caacataaaa
     1861 ttgtaactcg tcagtctaac aaaaatattt tacagtttgc tagatattta tctgatagag
     1921 gtactccggt atggattcgt catgttttag ttcctggatt gactgatatt gatgaccatt
     1981 taaaacgttt gggtgaattt gtccaaacac ttgataatgt cgataaattt gaagtattgc
     2041 cttatcatac tatgggagag ttcaaatggc gtgaattagg aattccgtat ccattagctg
     2101 gtgtgaaacc accaactcca gaacgtgtta aaaacgctaa agatatcatg aaaacagaat
     2161 cctatacaga atatctaaaa agaattcaaa attagtttta agttgaattt tattcaactt
     2221 ttttagttgc aaagggcttc ctagtaagat tttttgctaa gttattcaat ttttgctaca
     2281 ataaggtgaa tataaaagaa gaggtaaaaa catgtctaaa attttagtct ttggtcacca
     2341 aaatccagat tcagatgcta ttggatcatc agttgctttt gcatatctag ctaaagaagc
     2401 ttgggggctt gacacagagg ccgttgcttt aggaactcca aatgaagaaa cagcatatgt
     2461 tttagattat tttggtgttc aagcacctcg agtagtagaa tctgcaaaag cagagggtgt
     2521 agagacagtc attttaactg accataacga attccaacaa tctatttctg atattaaaga
     2581 tgtgacagtt tatggtgttg ttgatcatca tcgtgttgct aactttgaga ctgccaatcc
     2641 gttatatatg cgtttggaac cagttggctc agcatcatcg attgtttacc gtatgtttaa
     2701 ggaaaatgga gtatcagtac ccaaagagtt ggctggttta ttattatcag gtttgatttc
     2761 agatactctt cttttgaaat caccaacaac tcatgcttca gatattccag ttgcaaaaga
     2821 actagcagag cttgcaggtg taaacttaga agaatatggt ttagaaatgc ttaaagcagg
     2881 tactaacctc tcaagtaaga ctgcagcgga gttaattgat attgatgcta aaacatttga
     2941 attaaatgga gaagctgttc gtgttgcaca agttaatact gtggacatta acgatatttt
     3001 agcacgtcaa gaagagattg aagttgctat tcaagaagca attgtcacag aaggttattc
     3061 ggattttgta ttgatgatta ctgacattgt taattcaaat tcagagattt tagctctcgg
     3121 ttcaaatatg gctaaagttg aagcagcttt tgaatttaca cttgaaaata accacgcttt
     3181 tcttgctggg gcagtatcac gtaaaaaaca agttgttcct caattgacag aaagctacaa
     3241 tgcttaatta atcatgtaaa acgggctgcg tcccgttttt ttagtcatac atttaggagc
     3301 gtttttatga tattaacgtt taatccagga aagttagaaa gacaagagtt ttttaaggag
     3361 cttattaact atctttggat acatgatgat gtaacactaa gacaaataaa gtcccacttt
     3421 acagactatt ctaaaattga ccgtttgtta gaagaatata ttaatcatgg atatatcctt
     3481 cgccaaaata aacgctacag ccttaatctt ccttttctga gttcgctgga tggattaaca
     3541 ttagatgatt tggttttcat agattctgct agtcaaatct atcagttatt acagaagcga
     3601 aaatttgtga caaatttaga taaccaaacc aatcacttgg tatttgtaga agagacagat
     3661 tttgaaagaa acactttgac tctttctaat tatttctata aactaactaa ctgttatcca
     3721 ctttcaagag aacaaaaaaa gctttatcaa cttttaggag atgttaattc ggaatatgct
     3781 ttaaaatata tgtcaagctt tatattgaaa tttcttcgca aggatagtgt taagcaaaaa
     3841 cgtacagata tttttatcca agctttagaa ttacttggat atattagctt aaaccaagat
     3901 actacctatc gtttaaatgc taaacttgat gttgaagctt taaagattta cttaacataa
     3961 aaaaagactg gaagtttgtc cagtcttttt tagtcaactt ttgtcatcaa ccagagaaaa
     4021 tagggggcac caattattga tacaaccaat ccagtgggta tacctgtacc aactaagagg
     4081 agacgaccta tagtatctga taccaatagg ataatagcac caataagaag acagctaggt
     4141 agtataattt tatggttatt tcctaatagt cgtctactta agtgcccagc gagtaagcct
     4201 ataaaagtaa tattaccaat taagacgacg ctaatagcag caaggctact tgccagcatc
     4261 aaagtatata ttctttcctt ttttaaatga agccctagag cgagagcagt ctgctcgttt
     4321 aggttcataa tattaaggtg gcgactgcga ctatatgcta atccccataa caaaatgaga
     4381 atcgggataa aagtgataat ggtagtccag gaactaattg taatttttcc actaagccat
     4441 tcaacgatca tatccatttt ttgattatta gactgactaa taatagaaat cataacgcct
     4501 gaaatcattg tagatattcc gataccagtg atgattaatc gtgtagggct aatattgtga
     4561 tttcttctgt atgacataag atagacaaga aaaatagtta ccaaccctcc aaacatagca
     4621 aataggggta aaaagtataa tatagttgga ttgctaacat taaatgttcc tatggttaga
     4681 gcgattacta atccagctcc agcattgata ccaagaatac cggaatcagc tagtggattt
     4741 ttggttaatg tctgtaatag caacccagca attcctaaag aagctccccc aaagatacag
     4801 acaataatac gaggtaaacg aatgttagta atgataaaaa ggaaggcatc atcagatttt
     4861 ccacttatta gttttaaaac gtcaataacg gaagtattag catagccaag tgaaattgat
     4921 actagtatta ataatagtag gataatgaca gatgatatta aaacaaaagg agctttattt
     4981 ttttgaatca tagatttttc ccttttctaa ttaaccatag gaaacatgga agccctacga
     5041 tactgattat agaactaatt ggtgtctctg aagggggatt tataatacga gatgataagt
     5101 ctacccagat catgaaagta gcccctgaaa atcctattaa aggtaataac agacgataat
     5161 ctttgggaat aaaaagctta ataaaatgtg gaataatcaa tccaatgaaa gaaacggttc
     5221 ctatcaacgc gactgatgaa gccgataaaa acaaaacaat aagtaataga aacgcagtca
     5281 ttaattgagt tttttgtccc aatgctttgg ccacagattc attaaggctc aagatagtta
     5341 attgatgtgc taggagttgt gaaataagta aacctaaaat aatgataggt gctattatga
     5401 ttagcatttt ccagttaatt tgggataaac cacctgcttg ccaaccgata acagttcgat
     5461 tgagtttaaa gtataatgta accacttgtc caacagacgt aaaaagtgtg gagatcatcg
     5521 ctccagctaa aatgagtcgt agttggtggt aacccttttg ctttgtataa gagagtgtga
     5581 agactaaaag acaagaaatg acagacccta gtaagcagac aatgagaatc gtactatagt
     5641 gcatgctccc taaaaaagca tatgcaacaa caagggctaa cccagctccg gcgttaatac
     5701 ctagtagtcc aggatcagca atggcgtttc gagtgactcc ctgcataata gcacctgctt
     5761 gtgacatagc tgcacctact aagatagctg caattatgcg tggtagtcta atgtcaaaaa
     5821 taacatcctg agcattagag ttttttaagg gattttttaa aacagttatt aaatcactag
     5881 tcttgaaatt gatagcacca aatcgtaagc tggcgtaaca accaataaga aatagtataa
     5941 gtgttatgaa aaaaacaagc caaaaatgat ttgacttatt tttttgaacg agtttttttg
     6001 aagctttaaa cataagagga gctttctttt aatttgtatt ttctttgata gcctttgtaa
     6061 atgattttaa ttgagcttct aaagatagag ggtcagagaa ataaaacacg tcgtagttac
     6121 tttctatgat gtgccctttt ttgacagctg gtaaattctt ccagacatca ctttctttaa
     6181 gtgatgaagc tgctttttta gtcgttttgt ttatattaac aagggcataa tctccaacgt
     6241 aatcaccgat tgcttcttgc gaaacggtaa accacccttt tttaaagaca tcttttttga
     6301 ctttttctgg ggcagcataa cctagtgaat catagattag ttctccaccg cgtccaaaat
     6361 tattaccata taaatagata tttttatcat aaaaatccat aatagtaaaa gtagtgttag
     6421 gctttaagat atggtgtaaa tcttttttgg cagcgagagt tttagttttc cattggctaa
     6481 cccactgatt agcttctttt tctttaccga atactttccc caaggctggc atcatatcta
     6541 aataattttg tgcaccatat ttaataacta aagttggtgc aatttttttc agagtattga
     6601 tgtttggatc ttgatcgaaa accatgatta aatcaggttt ttgtgcggca atagcttctg
     6661 tatcatcagc agttaatttt ttagcttctt tcagttgctt accaaaaacg gggctatctt
     6721 tttctaagtc taaactgtaa cttgaaacat taacacctag ttttaataaa tacccagtgt
     6781 aagaatatgt aaaattaatt acttttttcg gattttcagg aatttttcca taataggtga
     6841 agccttcaat tttaggcatt ttagaaatag ttgttttagt gctttcttct ttagtttgtt
     6901 gtctgcaaga taccaaaaag aaggtcagta gtgtgaggac aataattcca atttttttca
     6961 taagataaca tctcctaaat aatattaatt ttgatcagtt gataattgat aagataacaa
     7021 aacaggttgt ttactaatag ggtcctgaat gatatgagca tcaatcttaa aaatatcttt
     7081 gataatatct ttagtcatga tctgactagg actaccttca tagataattt taccggtttt
     7141 cattgcaact agataatcag aatagcgcgc agacaggttt aggtcgtgta ggaccataat
     7201 aattgttttt tgcgtttcgt tattaagttt tttgagcaat tctaagactt ctaattgatg
     7261 attcatgtca agataagttg taggttcgtc taaaaagata gtatctgtat cttgtgctaa
     7321 agccatagca atccatactt tttgcctctg acctcctgaa aggtcatcaa catctctatt
     7381 agcaaattgc gctacgttag tcatttccat cgcccaatgg attttactac gatcatcatt
     7441 tgtcaaattg ccaaggtatt tttggtgtgg gaaccgtcca taagatatta gttcataaac
     7501 tgttatccct tcagttgctt ctaacacctg tgggagaagt gcaatcttct tagcaatttc
     7561 ctttgtgggt aaatgtgcaa tatcatgacc atcaatagtg atagtaccat cttttatttt
     7621 atggatccgt gtgagagcct tcaaaagagt agactttcca catccattgg caccaataat
     7681 cgttgttatt ttttggttaa gtatactgag ggaaagatta ttgatgattt ctttctgatc
     7741 gtaagataca ataatgtttt ctgcttttat gtgagacata gagaaccttt ctagcaaaaa
     7801 attaagttca ttataacata gaaaaaaggc ttacaaaagc cttttttagc attaattaag
     7861 aaaaatgtga acgaaatgat aattatttag aatgattcaa aatagtatca ataatcatta
     7921 ttgtttagct ttttttatca tttaaggtat tttttcgcaa cttctaaatc tccagagtaa
     7981 ttgtcacgat ttcctttgat aatttgataa gcatctgccc ctttaccagc gataataatt
     8041 gcatctagtt tgcaattagt aagagtactg gcattagcaa ttgcttcttc tcgatcaatg
     8101 atgatagtaa ccggacgatt aatatggctt gcgatttctt gtgagataac taagggatct
     8161 tcaaaattag gatcatctgc agtgagtatc acttgtagat taggatgttg attaatgaca
     8221 tctccgaagt ctttacgacg gctttgacct ttattaccag gagctccgag cactaatata
     8281 atatcccctt tttggtgttc ctcaacaaca gctaataatt tcttcaaact gtctccgttg
     8341 tgggcatagt caacgaaaat ctttgcgccg ttggtttgtg ttaggacttc catgcggcca
     8401 gggacggtag tttgtgcaat tccgtttttt atatcttcaa ttgaaacacc aagtcggaga
     8461 caagcaagtc cagctgcgag agcattttct tgattgaatt ttccaatcag gttgatattg
     8521 taggtgttct ccaactttcc tttgacatgg aaagaaaagg cctgtgattc tgtgataaca
     8581 ttttcagaat aatctccata gaaatcatga ggaatatatt ctacctgttc tttgacaata
     8641 ttaaaatgat ccatctgact atttacaacg acagcattgc tattctccat caagagccgt
     8701 ttgtggaaaa aataatcttc gaatgtaggg tgctcgatag gaccaatatg atcgggacta
     8761 atatttaaga aaacgccaac gtcaaaggtt aaaccataaa cacgtttggt taggtatgct
     8821 tggcttgata cttccatgac taagtgtgtc atttgattct caacggctgt agccatcatc
     8881 cgaaataaat caagactctc gggggttgtg agatgtgatt taaagaaaga tttaccatct
     8941 aatgttgtgt tcatagttga gagcatagca ggacgatggt ttacttttag catatgataa
     9001 gcaaagtaag ccgcagttgt ttttcctttt gttccagtaa aagctagaag tttaagtttg
     9061 ttttgtgggt tattatagaa agacatagat atcaaactca tggctttttt gatatcattg
     9121 actaatatca ctggaatatc ggcgccgtaa tctatctcag aaacgtaaaa agaaaggcct
     9181 gctgtgattg cagaatctag atattctttt ttaaaagtgg cacccttagc aaaaaataaa
     9241 gtatcagatg aaatttgacg actatcatat gataaggcat taaaagttac attttgtgtc
     9301 cagttataat aataatgctc atgaaacaaa atctctctga aattatggtc attttttaaa
     9361 atttctaaaa ttttatctat tgtaatcata ctccttattt tagactttat gttctcgttt
     9421 tacaagtcta aggtaaaagt ttataataaa gtgatatttc tgagattttt gtcttttaaa
     9481 tgctaaataa tattgaaaat agaaagttta ccatgtcaca aaaaacaaca aaagttagtc
     9541 agcaagaaca aatggtcaaa gggacggctt ggttaacagc agggaatttc attagtcgct
     9601 tgctgggagc catctatatt atcccttggt atgtttggat gggaaagcat gctgccgaag
     9661 caaatgcttt atttgggatg ggatatgaaa tctatgcttt atttcttttg atatcaactg
     9721 ttggaattcc agtagcagtt gcaaaacaag tatctaagta taacacttta ggtaaagaag
     9781 agatgagcat ttatctggtt cggaaaatct tgcagtttat gctgatttta ggtggagttt
     9841 ttgcattaat tatgtatatt ggttcacctc tatttgcttc acttagtaaa ggagggcaag
     9901 aactcgttcc aatattgcgt agtctaactc tagctgtttt agtttttcct tcaatgagtg
     9961 ttttacgtgg tttttttcaa ggttttaata atttaaaacc gtatgccatt agtcaagtcg
    10021 ctgagcagat tatacgtgtc atatggatgt tattaactgc tttctatatt atgcgtctag
    10081 gatcaggaga ttatattgct gctgtgacac agtcaacatt cgccgccttt gtcggtatgt
    10141 ttgctagtat agctgtgctg ctttatttct tatggcgtta taatatgcta tctgccttga
    10201 ttagtaaaac tccagagcac attaaattag atactaaaga aatcctaatt gagactgtta
    10261 aggaagcaat cccttttatt attactggtg cagcaattca aattttcaaa ttgattgatc
    10321 agttttcatt tgggaatacg atggcgctct ttacgaatta ttcaagtgaa gaattacggg
    10381 ttatgtttgc ttacttttca agcaaccctg gtaaagttac gatgattcta attgctgttg
    10441 ctaccgcaat tgctggagtt ggtattccat tgttaacaga gaattttgtt aagaatgata
    10501 aaaaagccgc cgctcgtttg gttgttaata atttacaaat gttactaatg tttttactgc
    10561 cagctgttgc aggatccgtt attttagcaa aacctttgta tacagtcttt tatgggttac
    10621 cacagggaca agctttaggg ctttttgtca tatctcttat tcaaacaatt attttatcta
    10681 tttatactgt tttagcacca atgctacaag cattgtttga aaatcgtaaa gctattattt
    10741 acttcctata tggtctggtg gcaaaagtta ttttacaagt tccctccatt ttccttttcc
    10801 atgcctatgg accacttttc tcaacgacag tagctctttg tatcccagta attcttatgt
    10861 atttgaaaat acatgaaatt acaggattta aacgccaagc tattcgtcgt acaagtgctt
    10921 tagtccttat tctaacgcta ttgatgagct ttattatttc tatgataata tggttaatga
    10981 acctggttat tgtacccgat agtagactgg taagtctagt ttacattatc gttatcggtg
    11041 caataggttt aggggtatat ggctttatgg cactggccac ccatctactt gataagatga
    11101 ttggtagtcg tgctcaagac ttacgccgaa agttgcacct taactaatga aaaaataggt
    11161 tagacagaat tgtttaacct agttttttga cgaccaaaat tatttgtgaa attttaaggt
    11221 agtcacaata tagtgagctt atttttatgt tataattaaa tggagaaata tgaggtgaaa
    11281 gatgtcttac gaaaagcttt tagaacgatt tttaacatac gtcaaaataa atacaagaag
    11341 taattctaat agtacacaaa cgccaacaac tcaaagtcaa gttgactttg ctttaacagt
    11401 tttaaaacca gaaatggaag caattggttt aaaagatgtt cattatttac cttctaatgg
    11461 gtatttggtt ggaaccttac ctgctacaag cgaccgctta cgccataaaa taggttttat
    11521 atcccatatg gatacagctg atttcaatgc tgaaaatatt actccacaaa ttgttgacta
    11581 taaaggtgga gatattgaac ttggagactc aggttacatt ttaagtccga aagattttcc
    11641 aaatttaaat aattaccatg ggcaaacatt gattacaaca gatggtaaaa ccttactggg
    11701 agcagacgat aagtctggta tagcagaaat catgacagct atggaatatt tggcttcgca
    11761 tccagaaatt gagcactgtg aaattagagt tggctttgga ccagatgaag aaattggtat
    11821 aggtgcagat aaatttgatg ttaaagattt tgatgttgat tttgcctata cagtggatgg
    11881 tggaccacta ggagaattac agtatgaaac ctttagtgca gctggtttgg agcttacatt
    11941 tgaaggacga aacgttcacc ctggaactgc aaaaaatcaa atgattaatg ctttacagct
    12001 tgctatagat tttcatagtc aattaccaga aaatgaacgt cctgaacaaa cagatggcta
    12061 tcaaggattt tatcacttat atgatttaag tggaacagtt gatcaagcta aaagttcata
    12121 tatcattcga gattttgagg aagttaattt cttaaaacgt aagcaattgg ctcaagctat
    12181 cgctgataat atgaatgaag cattacaatc tgaacgtgta aaggttaaac tatacgatca
    12241 atattacaac atgaagaaag ttattgaaaa agacatgaca cctatcaaca ttgctaaaga
    12301 agtaatggaa gagttagaca tcaagccgat catagaaccg attcgtggtg gtacagatgg
    12361 ctctaaaatt tcctttatgg gaatccctac tcctaatctt tttgcaggtg gtgaaaacat
    12421 gcatggacgc tttgaattcg ttagtctaca aacaatggaa aaagcagttg atgttatttt
    12481 aggcatcgtt gctaaggatt agtaaagaaa tatcagattg gagtggttaa atgataaaat
    12541 tatttgggaa gatacgttat cactggcaac ctgaactttc ttgggctatt atttattggt
    12601 caatagccat tgcaccaatt tttattggtt tatctctgct atatgaaaga actgagattc
    12661 cgagtcaggt atttgttcta tttgccatct ttattgtttt agtaggcatt ggatttcatc
    12721 gctattttgt tattgaagaa gatggatatc ttaggattgt atcttttaac tttttaagac
    12781 gatctaagtt tccaattgaa gatattgcta aaattgaggt cactaaatca agtgtcacta
    12841 tcaaatttaa taataaccat gagcgtattt tttacatgcg aaaatggcct aaaaaatatt
    12901 ttttagatgc actagctatt gaacctaaat ttaagggaga agtggagctt ctcgataatt
    12961 tgattaaaat ggattatttt gaatgttatc gctacgataa aaaagctctt acaaaatcgt
    13021 aagagctttg gtgggacatg atttaactgc taaaacagta tcttgatcag aacttggaat
    13081 ttctttttct aggttatctg tatcggaaaa tttaacgata ccatcatctt gataatcaaa
    13141 aatatttgaa tatgtttggc aaagcccgca ggcaatacat ttttctggaa ttagtgcaac
    13201 tttcatagta ttattgtaat acttattgat acgattaaca agggggaagt catggcgaaa
    13261 aaaccatggg aaaaaaaagt tgtggaaaac aatagtcata gaaaagacaa aattacaaga
    13321 acgagtagag gtgtggtgtc aagtactcct tggattaccg ctttcttgag tgcattcttt
    13381 gttattgtcg ttgctatttt atttatagta ttttatactt caaatagagg agaagacaga
    13441 gcaaaagaaa cttctggttt ttatggtgct tcaagtcaaa aagttaatag ttctaaaacg
    13501 aaaaaagcat ctacaagtaa gaaaacaacg tcatcatcaa aaactaaaac tagtgataaa
    13561 tcaacaacat cttctagtac agtagcaagt tcgagtgaga catcgacatc atcttcagta
    13621 aacggtggaa caatcgtagt tcaaccaggt gagggagcag cctccatagc tgctcgtgca
    13681 ggtatctcag ttgctcgttt acaagaactt aatcctggtc atatgacctc agggtattgg
    13741 tatgctaatc caggcgatcc tgtggttgta aattaagggg aggattatga actcaattaa
    13801 tattgctatt gatggaccag catcaagtgg gaaaagtact gtcgctaaga ttattgctaa
    13861 aaatttgaat tacacttacc ttgatacagg tgctatgtat cgttgtgcta cttatctggc
    13921 tttacagcat ggatatgaag cacaagatgt ttcgaaaatt ttaggactcc tagctgaaag
    13981 accgatttct tttggtaaag cagaagatgg cagccagaca gtctttcttg gcacagaaga
    14041 agttacttta gctatccgtc aaaacgatgt tactaacaat gtctcgtggg tttctgctat
    14101 cccagagatt cgagaagagt tagtaaacca gcagcgtcgt atagccaaag atggtgctat
    14161 tataatggat ggtcgtgaca taggaacagt tgttttgcca gatgcagaat tgaagatttt
    14221 tttagtggct tctgttgatg aaagagctga gcgtcgcttt aaagaaaatc aagaaaaagg
    14281 cattgagagt gattttgaaa cactgaaatc agaaattgct gctcgtgatt ataaagatag
    14341 ccatcgagaa gtttcaccat taaaggcagc agaagatgct attgaatttg atactacagg
    14401 agtatcaatt gaaggtgttg taacttttat tcaagaaaaa gcagaaaaaa taattgacat
    14461 gaagaattag tcatgatata ctagttgcaa tgagaaaagc agaagtgaga acttctcgcc
    14521 ttgcgactta ggttgtctgg cccaacatgt caagattcca tctaggaaga ttatatgtgc
    14581 gggcttgatt tatcaagtcc gctttcgttt ttctctaaaa aaataaagag gtgaagatca
    14641 tagctaaaaa agatctattc attaacgatg aaattcgcgt tcgtgaagtt cgtctggttg
    14701 gtctcgaggg tgaacaatta ggtattaaac cattgtctga agctcaagca attgcagatg
    14761 atgctaatgt tgatttggtt ttaattcagc cacaagctac accacctgtt gcgaaaatta
    14821 tggactatgg taagttcaaa tttgagtatc aaaagaaaca aaaagaacaa cgtaaaaaac
    14881 aaagcgttgt tactgttaag gaagttcgtc ttagcccagt tattgataaa ggggattttg
    14941 aaacaaaact tcgtaacggt cgtaagttcc ttgaaaaagg taacaaagtt aaagtttcta
    15001 tccgattcaa aggacgtatg attactcata aagaaattgg agcaaaggta ttggcggaat
    15061 ttgctgaagc gacacaagat atcgctatta ttgagcaaag agctaagatg gatggtcgcc
    15121 aaatgtttat gcagcttgca ccaattccag acaagaaata attgtcgact ttatagtatt
    15181 atttaagagg agaatttaaa atgccaaaac aaaaaacaca ccgcgcatca gctaaacgct
    15241 ttaaacgcac aggttcaggt ggattgaaac gcttccacgc gtttacttca caccgtttcc
    15301 acggtaaaac taaaaaacaa cgtcgtcatc ttcgtaaagc tactatggtt tcatcaggag
    15361 atttcaaacg tattaaagca atgcttactc gccttaaata ataaggtctc tgtaaacatt
    15421 tactacatat tataaaggat tatttggagg aaatataaat ggctcgtgtt aaaggtggag
    15481 ttgtttcacg taaacgtcgt aaacgcgtac ttaaattagc taaaggttac tatggtgcaa
    15541 aacatatctt gttccgtact gcaaaagaac aagtaatgaa ttcttactac tatgcatacc
    15601 gtgaccgtcg tcagaaaaaa cgtgacttcc gtaaattatg gatcactcgt atcaatgcgg
    15661 ctgctcgtat gaatggttta tcatattcac aattgatgca cggtttgaaa ttagctgaaa
    15721 tcgaagttaa ccgtaaaatg cttgctgatt tagcagttaa cgatgcagca gctttcacag
    15781 ctcttgcaga tgcagctaaa gctaaacttg gtaaataaga aataaaaaag ttgggtctgc
    15841 ctaacttttt ttaattacta gaaagaaagt acctatatta taatttaagt gaaaagtttc
    15901 gttctatata tgtagaatga aacttttttt gttttattcc gataccttta ttattgttat
    15961 aattttcctt tttgattttt attgttttat agtattattc cgtataaaaa gcacctcaat
    16021 cgttggattg aggtgtggat taaactttaa atggtaaaga ttatttttta gatgttttat
    16081 gtgtcttatt aggatcttgc aattccaagt aacttggagc gtggaagaga tcaactgttg
    16141 atttgttgtt atgtttgctg tatatgcttg ttgaacgatc tttcaattta cgttctttag
    16201 ctttgagtgc tttaagagaa tgtgtgtaat tgaatttttc aacttctaca gttttcaaac
    16261 cattgttttc atcaaatcgt agtaaatctc cagtttgaat cgaatcactt gtagataatt
    16321 gtttattagt agcatctcga atagttttta tttcagcttt agttgtttca tcaggatttg
    16381 tgatttcttg tcctgaatca gtgtaataga gatgtccact atagttagtg tatttaggag
    16441 taatatactg accagttgtt ctcatagcga ccatttgttt attatcttta gataacaagt
    16501 cttgtcctaa ctgagtatat ttagacgtgt ctatccctag aatatgaagt aaggttggta
    16561 aattatctac ctcaccaccg tatgtattac tgataaaacc ttttgaatag ccaggaatgt
    16621 ggatcatata tggaacacgt tgtaacatag cgttgtcgta ttcagaccat gtttcagggt
    16681 ctttacctaa taattcagct aagctaggat tgcgtgtatt agaaataccg tagtgatccc
    16741 catacattac aataatagaa ttatcataca caccagcagc ttttagatat tcaaaaaatg
    16801 atttaagagc tgtatcaagg tagtttgctg tggcgaaata accgttaatc gtttcatcgt
    16861 tagtctttgc aagtgggaat ccctcttcgt cagactctcc cttcaaactt gtgtaagggt
    16921 aatggttact tactgtaata aatttagtat aaaacggttg ttgcatgtgc tctagatatt
    16981 tgatagagtc agcaaacata tatttatcat tcaaaccgta ttggaatgaa ttatctttgg
    17041 tttgttttga gaagtagcta gagtcaaaga agtagtcata accccactgt ttgtaagcat
    17101 tatttctatt ccaaaatgtt ccgacgtttc cgtggaagac cgcactactg taacctccat
    17161 tttgagctaa gatatgtgga gcagcaaatt gtgtattttc accaccataa ttaaccatga
    17221 atgaaccact acttagacca aaaagtgagt tttccatgag tgtctcagca tcggatgtct
    17281 taccagcttt gacttgatgg aagaagttag agaaggagat agtctctttc gaatgataaa
    17341 ggctgttaat aaatggagta accacatgtt ctttaccatc aacatttaat ttatagtcaa
    17401 tcaagaattg ttgaaaactc tccaaatgaa tcatgataac gtttcgccct tttccgatac
    17461 cataatattc tggattgggt ttggcataat gctctgctac atattttttg gcagtcgcta
    17521 attcttgtgc agtggcacca tttctttctt tctgtgcttg ataagtttga ttaccactat
    17581 aaatcgaaaa tgatggcaag ccaagagctt taacaatata ggtgtttgaa aaaccacgac
    17641 ttagtagttc tggacgatca atttcagcta agaataagtt aattgaaaag agtaaaccag
    17701 acaaagctgt gatagaaaaa ctagcacgtt tattaaatgg acgattatct aagtggattt
    17761 tcttggttgc aaataagaaa ataaggataa tgaaatcgaa aacgtagaca agatcccaaa
    17821 ttctcaatag attaagagca gagtctccaa ggccagccga agttttacta ctagccaaga
    17881 cagcgctaac tgtaatgaaa tcggaaaact ctctatagta gattgcgttt gcaattagaa
    17941 ggatatttac gatagcatag gtgataaaag cggtaatata aaaagctttg gttcgcttaa
    18001 catataaggc caaacctatg attaagagtc ctaaggggat ggggtttata atggtcaaaa
    18061 acaactgata ggaattttct aaacctaaat taaaatcagt atggtaagcc caaattgtct
    18121 ttaaccaata tataactact aacaataaaa taaagccgag tcgtgtattg atcgcacggc
    18181 tagcaaaatc tttaattttt ttcacagtag gaacttcctt taattttttt cctaaagata
    18241 aaatctcaga ttaaattata acataaaatt ttgtaacata cggtaaagaa aggtcgaatt
    18301 cagtatgaaa aaagataaaa atttgttata atactgttta tgaataaact taaagtgaat
    18361 tctgtcgttg aaagaaaaat caaatcaggt gctcagttac tggaaaaaaa agattttgat
    18421 accagtttag ttaaccagtt ggttcaactt ttttcacagt caaatcaatt cttagggatg
    18481 gcctatcttt caccacaaaa taaagggatt ggttggttac tatcaagaca aatttttgat
    18541 tttaaccatg attactttgt atcgctattc gaaaaatcta gagagaagcg tcaaaaattt
    18601 gaaaaatcta gccaaacaac agcctaccgc ttgtttaatc aggatggaga taactttggt
    18661 ggcctaacta tagattttta tagtgactat gctctttttt cgtggtacaa tgaatttgtt
    18721 tatactaatc gacaaatgat tgttgcagcc tttaagcagg tctatcctaa tattaaaggg
    18781 gcatatgaaa aaattcgttt caaaggttta gactttgaaa gtgctcattt gtacggtcaa
    18841 gaggctcctg aatcattttt gattttagaa aataatatca aatatagtgt ctttttgaat
    18901 gatgggttaa tgacaggtat tttccttgac caacatgatg tcagaaaagc cttagcaact
    18961 aatctatcag aaggtaaaaa agttctaaat atgttttcat acactgcggc tttttctgta
    19021 gctgcagctg ttggaggagc attagagaca acttctgttg atttagcaaa acgctcgcgt
    19081 gaactttcaa aagcacactt tgatgctaat cagattgtca cagataacca tcgatttatc
    19141 gttatggatg tttttgaata ttataagtat gccaaaagaa aacatctatc atatgatgtg
    19201 attgttattg atccaccaag ttttgctcga aataaaaaac aaactttttc ggttactaaa
    19261 gattactata aattaattga acaagcttta gatattttag cccctggtgg aactatcatt
    19321 gcatcaacta acgcagctaa tctaaccgta tcacaattta agaaacaatt ggaaaagggg
    19381 tttggtaaag cttcacataa ttacattagc ttacagcagt tgcctgaaga tttcactgta
    19441 aatgataagg accaacaaag taattattta aaagtattta caataaaggt aaaataatga
    19501 aaatagtagt accagtaatg cctcgcagtc ttgaagaggc tcaagaaata gatttatcaa
    19561 aatttgatag tgttgatatt attgaatggc gagctgatgc cttaccaaag gatgacatta
    19621 ttaatgtagc tccagctatt tttgagaaat tcgcaggtca tgaaattatt tttactcttc
    19681 gtacaacgcg tgaaggtggt aatattgtct tatctgatgc tgagtatgtt gagttaatcc
    19741 agaaaattaa ttctatctac aatccagatt atattgattt tgagtatttt tcacataaag
    19801 aagtttttca agaaatgcta gaatttccaa atttagtcct gtcttatcac aattttcaag
    19861 agacaccgga gaatattatg gagatatttt cagaattaac agccctagca ccacgagttg
    19921 tgaaaatcgc agtaatgcca aagaatgaac aagatgtctt agacgttatg aattacactc
    19981 gcggtttcaa gactattaat cctgatcaag tttatgcgac ggtatctatg agtaaaattg
    20041 gacgtatttc tcgttttgct ggtgatgtaa ctggatctag ttggacattt gcatatttag
    20101 attcatctat cgcacccgga caaattacta tttcagagat gaagcgtgtc aaagcattgc
    20161 ttgacgctga ctgatacgag ataacaaaat gttaaatata ctgatgagat ccctagcaat
    20221 tgcttagggt ttttgtctta ttaaaacaaa catgagagga cattagaatg caggttgaag
    20281 ttgacttacc gaatcaccct tatcatatta aaattgaaga aggatgtttt tctgaggctg
    20341 gtgactgggt atcacattta tggcagaaac aaatgataac tattattaca gatagtaatg
    20401 tagaaatatt atacggggag tcgcttgtca accagttaaa aaaacaaggt tttacagtac
    20461 atgtttttag ttttgctgcg ggtgaagcta gtaaaacttt ggaagttgct aatcgtattt
    20521 atggtttttt agcaaagcat catatgacca gaagtgatgg gatcattgca cttggtggag
    20581 gcgtcgtcgg agatttagca gcgtttgttg cttctactta tatgcgggga atccattttc
    20641 ttcaaattcc aactagttta acagcacagg ttgattcttc cattggcgga aaaacaggag
    20701 tcaacacttc attcgctaaa aatatggtag gtacttttgc acaacctgat ggtgtactga
    20761 tagatcctgt tactctaaaa acattgggca atcgtgaatt agttgaaggt atgggagaag
    20821 tcatcaaata tggcttgatt gatgatataa aactctggca tatcttagaa gagatggatg
    20881 ggtctattga tagtatcctt gataatgcat tagctattat ttatcactct tgtcaagtta
    20941 aacgaaagca tgttttagct gatcaatatg ataaagggtt aaggatgcat ttaaattttg
    21001 gccacactat cggtcatgct atcgaagtac atgctggcta tggagaaata atgcatggag
    21061 aagctgttgc aattggaatg atccagcttt ctcgtgtggc tgagcgtaaa aatcttatgc
    21121 caagaggtat cagtcaagat atttataata tgtgtttaaa atttggcctt ccggtccact
    21181 atgcagaatg ggataaggac gtcttattta acatattgag tcatgataaa aaagcaagtg
    21241 gacaatttat taaaattgtt atactgcctc aattaggtag tgcaacagtc caccaaatac
    21301 ctttagaaga aatgagagat tatttggaga aatagatgag atatttaact gctggtgagt
    21361 cacatggacc tagtttaaca gctattatcg aagggattcc tgcgggactg aaattatctg
    21421 ctaaagatat taatgaagat ttaaaacgcc gccaaggtgg ttatggtcgt gggaatcgaa
    21481 tgaaaattga aactgatcaa gtgatcatta gttctggtgt gcgccatgga aaaaccttgg
    21541 gttcacctat tacactgaca gtaactaata aggatcatag caaatggctg gacatcatgt
    21601 cagtcgaaga cattgaagag cgtctaaagc aaaaacgtcg tattaagcat cctagacctg
    21661 ggcacgcaga tttagtaggt ggaatcaaat accgatttga tgatttgaga aatgctttag
    21721 agcgttcttc ggctcgtgag accacaatgc gagtagctat tggtgcgatt gctaaacgta
    21781 tcttgaaaga aattggtatc gaaattgcta accatattgt agttttcggt ggcaaggaga
    21841 ttactgtccc tgataagcta acagttcaac aaatcaaagt gttatctagt caatcgcaag
    21901 tagctatcgt caatccatca tttgaacaag aaattaagga ttatattgac agtgtgaaaa
    21961 aggctggaga tactatcgga ggagtgattg aaactatcgt tggaggagtt cccgttggtt
    22021 taggctctta tgtacactgg gatagaaagt tagatgctaa gatagcgcaa gcagttgtat
    22081 ctatcaatgc ttttaaaggt gttgaatttg gtctaggttt taagtcaggg tttttaaaag
    22141 gaagtcaagt aatggactcc atatcatgga caaaggacca aggttacata cgtcagtcaa
    22201 ataatttagg cggttttgaa ggcggtatga ctaatggaga gcctattgtc gttaggggag
    22261 taatgaagcc aattcctacg ctttacaagc cactaatgtc agttgatatt gatactcacg
    22321 agccttatag agctacagtg gaacgttctg atcccacggc acttccggct gcaggtgtcg
    22381 ttatggaagc tgtagtagcc accgtcctgg tgacagaagt gctagaaaaa ttttcttcag
    22441 acaacatgta tgaactaaaa gaagctgtaa aactatatcg caattatgtt aatcattttt
    22501 aagtgaaaac tagtagcatt caattgcaaa aaaaggtata aaagtctaca atagtggtaa
    22561 gttcaagtag aaaaaggagt aacttatggc taatgtatat gatttagcaa atgaattaga
    22621 acgtgctgtt cgtgctttac cagaatacca agcagtttta actgcaaaag cagctattga
    22681 aaatgatgcg gatgcacaag tgctttggca agactttttg gctacccaat caaaagttca
    22741 agaaatgatg caatctggcc aaatgccaag tcaagaagaa caagatgaaa tgtctaaact
    22801 tggggaaaaa attgaatcca atgacctttt aaaagtttat tttgaccaac aacaacggtt
    22861 gtctgtctat atgtctgata tcgaaaaaat tgtctttgca cccatgcagg acttgatgta
    22921 atagtataaa agagagagct ccttgagttt tctcttttat gctatttaca ttcagagcat
    22981 cctaattgct gcgcttttac attattgcat tatcctataa aaatgtattt ttatactttt
    23041 tttgagagcg gctatttatt tttagactta gtcatgtctc taaatacccc tagttagtga
    23101 taaaaacaag gtttttatag gataaggagg t
//
LOCUS       DATROQ010000027        22820 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.027, whole genome shotgun sequence.
ACCESSION   DATROQ010000027 DATROQ010000000
VERSION     DATROQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22820
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.027"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            33..257
                     /locus_tag="VBC94_000916"
     CDS             33..257
                     /locus_tag="VBC94_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6979195.1"
                     /translation="MAVFEKVQEIIVEELGKDAEEVTLNTTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKVK"
     gene            412..1371
                     /gene="fabK"
                     /locus_tag="VBC94_000917"
     CDS             412..1371
                     /gene="fabK"
                     /locus_tag="VBC94_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEO6979196.1"
                     /translation="MKTRITELLNIKYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSMTDKPFGVNIMLLSPFVDDIVDLVIEEGVKVVTTGAGNPGKY
                     MERFHEAGITVIPVVPSVALAKRMEKLGADAIITEGMEAGGHIGKLTTMTLVRQVVDA
                     VTIPVIAAGGIADGRGAAAGFMLGADAVQVGTRFVVAKESNAHPNYKAKILKAKDIDT
                     AVSAQVVGHPVRALKNKLVTTYSQAEKDYLAGRISINEIEELGAGALRNAVVDGDVIN
                     GSVMAGQIAGLIKSEETCQEILEDIYSGARQVILSEASRWSDL"
     gene            1391..2317
                     /gene="fabD"
                     /locus_tag="VBC94_000918"
     CDS             1391..2317
                     /gene="fabD"
                     /locus_tag="VBC94_000918"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEO6979197.1"
                     /translation="MNKVAFLFAGQGAQKLGMARDLYETFPIVKETFDKASHVLGYDL
                     RELIDKDLDKLNQTKYTQPAILTTSTAIYRLILKEIGLRPDMVAGLSLGEYSALVASG
                     AIRFEDAVVLVSRRGQLMEAAAPAGSGKMVAVLNADRQIIEDACKKASQFGIVSPANY
                     NTPKQIVIGGESIAVNAAVEELKQQGVKRLIPLNVSGPFHTALLKPASQKLSDVLDKV
                     HFSVSEIPVIGNTEAQIMKKDDIKSLLARQVMEPVRFDESIETMKKMGMTQVVEIGPG
                     KVLSGFLKKIDSSLSVYSVEDKIGFNNLKELN"
     gene            2326..3060
                     /gene="fabG"
                     /locus_tag="VBC94_000919"
     CDS             2326..3060
                     /gene="fabG"
                     /locus_tag="VBC94_000919"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEO6979198.1"
                     /translation="MQLKDKNIFITGSSRGIGLAIAHQFAQLGANIVLNGRSEISEDL
                     IAEFADYGVKVIAISGDVSSFEDANRMIKEAIASLGSVDVLVNNAGITNDKLMLKMTV
                     EDFESVLKINLTGAFNMTQSVLKPMTKARQGAIINISSVVGLTGNVGQANYAASKAGL
                     IGFTKSVAREVAARGIRVNAIAPGFIESDMTDVIPEKMQEAILAQIPMKRIGKGKEVA
                     QVASFLAEQEYLTGQVIAIDGGMTMQ"
     gene            3076..4308
                     /gene="fabF"
                     /locus_tag="VBC94_000920"
     CDS             3076..4308
                     /gene="fabF"
                     /locus_tag="VBC94_000920"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEO6979199.1"
                     /translation="MTLQRVVVTGYGVTSPIGNTPEEFWNSLKEGNVGIGPITKFDSS
                     DFMVKNAAEIHDFPFDKYFVKKDLNRFDMYSLYALYASSEAIQHANLNLDEIDADRFG
                     VIVASGIGGIQEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMRLGAHGVCKSI
                     NTACASSNDAIGDAFRNIKFGIQDIMVVGGAEAAITKFAIAGFQSLTALSTTEDPSRA
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     LGATKAIQLALAEANIKPEEVNYVNAHGTSTPANEKGESQAVVAALGTDVPVSSTKSF
                     TGHLLGAAGAVEAIATIEAIRHSYVPMTAGTTELSEDITANVIFGQGQDADIRYAISN
                     TFGFGGHNAVLAFKRWED"
     gene            4274..4810
                     /locus_tag="VBC94_000921"
     CDS             4274..4810
                     /locus_tag="VBC94_000921"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEO6979200.1"
                     /translation="MQFWPSNAGRIKLDIQEIKDLMTQFDESSLREFSFKTSDGELSF
                     SKNEGKAPLVPTMSPMSHQPEATPTIATPVSNEAGEQTKQATEVVSEIPESTVTVAEG
                     DVVESPLVGVAYLASGPDKPNFVSVGDSVKKGQTLMIIEAMKVMNEVPAPHDGVVTEI
                     LVANEEVIEFGKGLVRIK"
     gene            4807..5229
                     /gene="fabZ"
                     /locus_tag="VBC94_000922"
     CDS             4807..5229
                     /gene="fabZ"
                     /locus_tag="VBC94_000922"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO6979201.1"
                     /translation="MIDIKEIREALPHRYPMLLVDRVLEVSEDEIVAIKNVSINEPFF
                     NGHFPEYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTAKFVKRRGTIAVVEAIAEVDGKLAASGTLTFAIGN"
     gene            5267..6637
                     /gene="accC"
                     /locus_tag="VBC94_000923"
     CDS             5267..6637
                     /gene="accC"
                     /locus_tag="VBC94_000923"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEO6979202.1"
                     /translation="MFKKILIANRGEIAVRIIRAAREMGISTVAIYSEADKESLHTIL
                     ADEAICVGPAKSAESYLNVNAILSAAIVTGAEAVHPGFGFLSENSKFATMCEEMNLKF
                     IGPSGEVMDKMGDKINARAEMIKADVPVIPGSDGQVTSVEEAVSIAEEIGYPLMLKAS
                     AGGGGKGIRKVKSADELKPAFESASQEALAAFGNGAMYIEKVIYPARHIEVQILGDSF
                     GKIVHLGERDCSLQRNNQKVLEESPSVAIGNTLRQQIGEAAVRAAEAVSYENAGTIEF
                     LLDENSGQFYFMEMNTRVQVEHPVTEFVTGVDIVKEQIRIAAGIPLSVSQNDIKLTGH
                     AIECRINAENPQFNFAPCPGTINGLHLPAGGMGLRVDSAVYTGYTIPPYYDSMIAKVI
                     VHGENRFDALMKMQRALYELEIDGIITNTEFQMDLISDKKVLAGDYDTSFLMEDFLPR
                     YYGNNQ"
     gene            6646..7521
                     /locus_tag="VBC94_000924"
     CDS             6646..7521
                     /locus_tag="VBC94_000924"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEO6979203.1"
                     /translation="MALFSKKDKYIRISPNKALGSSDKRSLPEVPDELFAKCPSCKHM
                     IYQKDLGLAKICPACSYNFRISAQERLLLTVDEDSFEELFTGIETKDPLNFPNYREKL
                     AATRQKTNLDEAVVTGLAKIKGQTTALAIMDSHFIMASMGTVVGEKLTRLFELATEKK
                     LPIVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNQGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQALVGFAGRRVIETTVREDLPEGFQKAEFLLEHGFVDAIINRTELRDC
                     IAQLIAFHGNEVGHE"
     gene            7514..8287
                     /locus_tag="VBC94_000925"
     CDS             7514..8287
                     /locus_tag="VBC94_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEO6979204.1"
                     /translation="MNNDVTRILKDARDQGRLTALDYAELIFDNFMELHGDRQFADDK
                     SIIGGLGYLAGRPVTIVGIQKGKNLQDNLDRHFGQPHPEGYRKALRLMKQAEKFGRPV
                     ITFINTAGAYPGVGAEERGQGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALAVAD
                     KVWMLEHTVYSILSPEGFASILWKDGTRTTEAAQLMKMTAGELYHMEVVDKVIPEHGY
                     FSSEIVDMIKTSLISELEMLSQLSLEDLLEQRYQRFRKY"
     gene            8755..9387
                     /locus_tag="VBC94_000926"
     CDS             8755..9387
                     /locus_tag="VBC94_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEO6979205.1"
                     /translation="MYQELNDYLEFQSMKYIMPEKAGPLKDDMQTFKAKGQLARNSFT
                     ELSRALEQRMDGFKMQRVSNWANQAQVGRPHFWVYYRKDTDQLDDVAVALRVYGVKDS
                     FGVSLEVSFVERQKSDKTLEKQARVLSIPIASPLYFMVQRQGETHREEGNEENRQRLM
                     QEIKSGKVRKVLVKYDVLLTENQSLENILQRLLEGFEKVLPYYQVCQTIS"
     gene            complement(9433..10710)
                     /gene="serS"
                     /locus_tag="VBC94_000927"
     CDS             complement(9433..10710)
                     /gene="serS"
                     /locus_tag="VBC94_000927"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEO6979206.1"
                     /translation="MLDLKRIRTDFDAVAKKLATRGVDQETLTTLKELDIKRRELLIK
                     SEEAKAQRNVASAAIAQAKRNKENADEQITAMQTLSADIKAIDAELADVDANLQSMVT
                     VLPNTPADDVPLGADEDENVEVRRWGTPREFDFEAKAHWDLGESLGILDWERGAKVTG
                     SRFLFYKGLGARLERAIYSFMLDEHAKEGYTEVIPPYMVNHDSMFGTGQYPKFKEDTF
                     ELADSPFVLIPTAEVPLTNYYRDEIIDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLNLPYRVITLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEVDGKVRLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGNIDIIKPN"
     gene            11009..12070
                     /locus_tag="VBC94_000928"
     CDS             11009..12070
                     /locus_tag="VBC94_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEO6979207.1"
                     /translation="MEKYTSIKQSGRHYGLDLLRIISMFMIVITHVLGKGGLRSSVEG
                     HADSYFIVTWIIQVLVYGAVNCYALISGYVGINSRYRYSKLLSIWTQVFFYTFTITAL
                     FAITGHEVTLLNWRDAFFPIVSGQYWYITAYFGLLVFMPVINNGLNALTDKQLKQLVL
                     LMFIIFSILPAVLNNRVPEFSLSKGFEMTWLLILYIIGAYLKRIDLNIFKTSYLLIVY
                     LLSLVATYVMKFSIGDIWYWYVSPTLTLGAVSLFILFARASIKPSGFLKKIIVVLAPS
                     TLGVYLCHLHPLIVKYFVRDFAETFVYESIYLYPFLILGAGILIYLLAFIVERIRLRI
                     FKAIHFSNFLRWFDDKFPY"
     gene            complement(12108..12470)
                     /locus_tag="VBC94_000929"
     CDS             complement(12108..12470)
                     /locus_tag="VBC94_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEO6979208.1"
                     /translation="MAQSLNKTVEFQTTGVSYLGMGNKVGKFLVGDQALEFYNDKNVN
                     DYIQIPWTSINQIGANVSRKKISRHFEVFTDQGKFLFASKDSGTILKHARRHIGDDKV
                     VKLPTLIQTILKIFKFKK"
     gene            complement(12589..13500)
                     /locus_tag="VBC94_000930"
     CDS             complement(12589..13500)
                     /locus_tag="VBC94_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6979209.1"
                     /translation="MTEQIKLSKSDRQKVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTTKEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEEKASGTPVEDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASAGNILGPIIFFVGWNLIRMSFLWYTQE
                     LGYKSGKEITKDMSGGILQDITKGASILGMFILAVLVKRWVAINFTVDLPKKTLSEGA
                     YINFPKDHVSGQQLHDILGQVQSGLSLDKMQPQTLQGQLDSLIPGLAGLLLTFFCMWL
                     LKKKVSPITIIIGLFIVGILARLAGVM"
     gene            complement(13515..14327)
                     /locus_tag="VBC94_000931"
     CDS             complement(13515..14327)
                     /locus_tag="VBC94_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEO6979210.1"
                     /translation="MADISIISMILVVIVAFFAGLEGILDQFQIHQPLVACTLIGLVT
                     GHLEAGIILGGSLQMIALAWANIGAAVAPDAALASVAAAIILVKSGDFTQKGINFAFS
                     TAVPLAIAGLFLTMIVRTISTALVHAGDKAASEGNFAAIERFHFIALLLQGLRIAFPA
                     ALLLAIPSSSVQSILEAMPDWLNGGMQVGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAALNQLTLIAMGTIGVAIALIYISLSKMGGSKGTSNAGSNDPIGDILEDY"
     gene            complement(14360..15370)
                     /gene="manX"
                     /locus_tag="VBC94_000932"
     CDS             complement(14360..15370)
                     /gene="manX"
                     /locus_tag="VBC94_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEO6979211.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIAQFDADDEVLVLADLWSGSPFNQASRVMGENPERKMAIITGLNLPMLIQ
                     AYTERMMDANAGVEQVAANIIKESKEGIKALPEELNPVVEATPVAGVPADVPAEVKQS
                     GSIPEGTVIGDGKLKINLARIDTRLLHGQVATAWTPASKANRIIVASDEVSKDELRKQ
                     LIKQAAPGGVKANVVPISKLIEVAKDPRFGNTRALILFETVQDALRAVEGGVEIPELN
                     VGSMAHSTGKTMVNNVLSMDKDDVAAFEKLRDLGVSFDVRKVPNDAKKNLFDLINKAN
                     VK"
     gene            complement(15672..16484)
                     /locus_tag="VBC94_000933"
     CDS             complement(15672..16484)
                     /locus_tag="VBC94_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6979212.1"
                     /translation="MTKKIIAVDLDGTLLHNNNTISDYTADTLRKVQAQGHKVIITTG
                     RPYRMALAHYLRLDLKTPMINFNGALTHIPEKKWAFEHSATIDKKLLLETLNLSDAIQ
                     ADFIASEYRKNFYITMDNRDKINPQLFGVNEITDKMALDVTKITRNPNALLMQTRHKD
                     KYELAKELRQHFNHELEVDSWGGPLNILEFSPKGVNKAYALKHLLKSLNLSQENLIAF
                     GDEHNDTEMLAFAHTGYAMKNASPTLLPYADQQIQWTNEEDGVAKTLEKLLL"
     gene            16573..17157
                     /locus_tag="VBC94_000934"
     CDS             16573..17157
                     /locus_tag="VBC94_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979213.1"
                     /translation="MKKLIWSHLFAIVSILGIWYYHVKIDHLMVILLASVLSLEMGFI
                     AYYSRNIFIRLLASLGLLFFYPQSLGIFLSLVSLEWSTKDIWQSNHFMQYMAYLLAIL
                     FIISSALLSLKLLVNAYDISKRYQLATVIPVMLFSSFAFCLEKSGSLGLEGIILNPSA
                     FFNGIQTNMANLDFKALSCLFLVQLLLWGILMED"
     gene            17247..17858
                     /locus_tag="VBC94_000935"
     CDS             17247..17858
                     /locus_tag="VBC94_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEO6979214.1"
                     /translation="MKVKLFAIHLFFIVISFGIKYYHLQGPNLIWNMTLALIALDFAY
                     LTSLFKKKILIGLFALAWFFFYPNTFYMLTDIIHMHFVGDVLYNKTNLILYILYVSSI
                     LFGFLSGIESFSVIMRKFRISNIFLRWGIIGIVSFVSSFGIHIGRYARLNSWDILTKP
                     QVVINELLAVPSRDSFHFILGFTFLQVLCLIFMDYSAGKKLEK"
     gene            17961..19382
                     /locus_tag="VBC94_000936"
     CDS             17961..19382
                     /locus_tag="VBC94_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEO6979215.1"
                     /translation="MEKFFKLKEHGTTIRTEITAGLTTFFAMSYILFVNPAILSQTGM
                     PAQGVFLATIIGAVVATSVMAFYANLPYAQAPGMGLNAFFTYTVVFALGYTWQEALAM
                     VFICGLISLIITLTKVRKMIIESIPTTLKSAITAGIGTFLAYVGIKNAGFLKFSIDPG
                     TYDVVGKGAAKGLATITANSSATPGLVSFDNPAILLSLIGLSITIFFIVKGIRGGIIL
                     SILTTTLLGILMGVVKLDAINWEATNLSASFRDLKQVFGVALGEKGLISLFSNPSRLP
                     SVLMAILAFSLTDIFDTIGTLIGTGEKVGILATTGDNHESKSLDKALYSDLIGTTFGA
                     ICGTSNVTTYVESAAGIGAGGRTGLTALVVAGLFAISSFFSPLVSIVPSQATAPILVI
                     VGIMMLSNLKDIKWDDMSEAIPAFFTSLFMGFTYSITYGIAAGFLTYTLAKVIKGQAK
                     DIHVVLWILDILFILNFISLAIL"
     gene            19465..19974
                     /gene="tsaE"
                     /locus_tag="VBC94_000937"
     CDS             19465..19974
                     /gene="tsaE"
                     /locus_tag="VBC94_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEO6979216.1"
                     /translation="MLWGCQNQQTVGRQRYFWYNISMFYTQNEEELIALGQKLGTVLK
                     SGDIVLLTGNLGAGKTTLTKGIAKGLDIKQMIKSPTYTIVREYEGRVPLYHLDVYRIG
                     DDPDSIDLDDFLFGQGVTVIEWGELLSDNLINNYLEIVITRSNQGRQVQLEAYGHRAR
                     EIIEAIQDV"
     gene            19928..20488
                     /locus_tag="VBC94_000938"
     CDS             19928..20488
                     /locus_tag="VBC94_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001890427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6979217.1"
                     /translation="MGIAQEKLLRRFKMSKQFAVDFIEASREEASALLEFLNTVTEET
                     DFILHTVSNQLSLPEMETFIENTLITKNCICLIAKLKNKVIGLITIISQSDIEIEHIG
                     DLFIAVQKDYWGYGIGHILMEEAIEWASDNDITRRLELSVQGRNERAIHLYQKFSFEI
                     DGLQTRGIKRENGEFLDIYRMSKLID"
     gene            20497..21804
                     /locus_tag="VBC94_000939"
     CDS             20497..21804
                     /locus_tag="VBC94_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6979218.1"
                     /translation="MKIWKKITLMFSAIILTTVIALGVYVASAYNFSTNELSKTFKDF
                     KLAKSKSHAIEETKPFSILLMGVDTGSEHRKSKWSGNSDSMILVTINPKTNKTTMTSL
                     ERDVLIKLSGPKNNGQTGVEAKLNAAYASGGAEMALMTVQDLLDINVDYFMQINMQGL
                     VDLVNAVGGITVTNKFDFPISIAANEPEYKAVVEPGTHKINGEQALVYSRMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALNSISSYKKILSAVSNNMQTNIEISSKTIPNLLAYKD
                     SLEHIKSYQLKGEDATLSDGGSYQILTKKHLLAVQNRIKKELDKKRSKTLKTSAILYE
                     DYYGTTASNDSSTYSSTQENNYNTTPYSEAPPSYSGNTTYSSETNQTTHQSYYNSSTP
                     ASNYSSNTNTGQADSSGSVNNYNGAATPNPNTGTQPVPSQTNP"
     gene            complement(21868..22164)
                     /locus_tag="VBC94_000940"
     CDS             complement(21868..22164)
                     /locus_tag="VBC94_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979219.1"
                     /translation="MKIGRLIALGLVSLGAFELYKNRKTIKDSYQNTKNETDSAKLKL
                     ERIKNDLAIISQEKEKIRLISQELNHKFQVFNKDIQPRLEEINQRMAKYQEKDE"
     gene            complement(22161..22580)
                     /locus_tag="VBC94_000941"
     CDS             complement(22161..22580)
                     /locus_tag="VBC94_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEO6979220.1"
                     /translation="MDNCIFCKIISGEISSSKVYEDDEVLAFLDITQTTTGHTLLIPK
                     KHVRNVLEMDEKTAQITFERLPKVARAVQAATKAKGMNIINNNEEIAGQTVFHAHVHL
                     VPRFDESDGIKIHYTTHEPDFEALAKLAKEIRKEIQL"
BASE COUNT         7189 a         3611 c         4614 g         7406 t
ORIGIN      
        1 taaaagatta tttaaaattt aaggagctaa aaatggcagt atttgaaaaa gtacaagaaa
       61 ttattgttga agaacttggt aaagatgcag aagaagtaac gttgaacact acttttgacg
      121 atcttgatgc tgattcgctt gatgttttcc aagtgatttc agaaattgaa gatgcatttg
      181 atatccaaat tgagacagaa gaaggactaa atactgttgg tgatttagtt gcttatgttg
      241 aagaaaaagt aaaataatta agtcgtaaga aaaaaagaag caagtgataa taaaagatat
      301 tcttaccaaa tcacttatta gagtgagtat tcaatcaatc gttgatcggt actcactctt
      361 cttcttatca atatagtttg attgtcaaat tattaatttt aggaaagaaa aatgaaaaca
      421 cgtataacag aattactaaa tataaaatat ccaatttttc aagggggtat ggcgtgggtc
      481 gctgatggag atcttgcagg agctgtttct aaggcaggtg gtctaggtat tattggtgga
      541 ggaaatgccc ctaaagaggt tgtcaaagca aatattgata aaataaaatc tatgactgat
      601 aaaccctttg gtgtgaatat tatgctttta tcaccttttg tagatgatat tgttgatttg
      661 gttattgaag aaggtgtaaa agttgtaacg actggtgcag gtaatcctgg taagtacatg
      721 gaacgctttc atgaagcagg tataacagta atccctgttg ttcctagtgt tgcattagca
      781 aaacgtatgg agaaattagg ggctgatgct atcattacag aaggaatgga agcgggtggt
      841 cacatcggga agctgaccac gatgacatta gtgcgacagg ttgttgatgc tgttacaatt
      901 ccagtcattg ccgcaggtgg tatagcagat gggcgtgggg ctgctgcggg tttcatgtta
      961 ggagctgatg ctgttcaggt aggaactcgt tttgttgttg ctaaagaatc taatgctcac
     1021 cctaattaca aagcaaaaat tttaaaagca aaagatatcg acactgctgt ttctgctcag
     1081 gtagtaggtc atccagttcg tgccttaaaa aataaactag taacaaccta tagccaagca
     1141 gaaaaagatt atttagctgg ccgtatttca atcaatgaga ttgaagaatt aggagcggga
     1201 gctcttcgca atgcagttgt tgatggggat gttattaatg gttcagttat ggcaggacaa
     1261 attgcaggat taattaaatc agaagagact tgtcaagaga ttcttgaaga tatttattca
     1321 ggagcgcgtc aagttattct ttctgaggca agccgctggt cagatttata aatacaaata
     1381 aggagacact atgaataaag ttgcatttct atttgctggt caaggtgctc aaaagttagg
     1441 aatggctaga gatttatatg aaactttccc gattgttaaa gaaacttttg ataaggcaag
     1501 tcatgtttta ggatatgatt tgagagaatt gattgataaa gacttagaca aattaaatca
     1561 gacgaaatat acgcaaccag ctattttaac gacttcgact gctatttatc gtttaattct
     1621 taaagaaatt ggacttagac cagatatggt tgcaggactt tctttgggag aatatagtgc
     1681 tttagttgct tcaggtgcta taagatttga agatgctgta gtacttgttt ctagacgagg
     1741 ccaactaatg gaagcagcag cacctgctgg tagtggcaaa atggtagcgg ttttaaatgc
     1801 tgatcgtcaa ataattgaag atgcttgtaa gaaggcttct cagtttggta ttgttagtcc
     1861 tgcaaattat aatactccaa agcaaattgt tataggggga gagtcaatcg ctgtaaatgc
     1921 agctgtagaa gaactaaaac aacaaggagt aaaacgttta attcctctga atgtttcagg
     1981 tccttttcac actgctttat tgaaacctgc aagtcagaaa ttgagcgatg ttttagataa
     2041 agttcatttt tcagtttctg aaatccctgt aattggaaat acagaggcgc agataatgaa
     2101 aaaagacgat atcaaatctt tattggcacg ccaagttatg gaacctgtac gctttgatga
     2161 gtctattgaa accatgaaaa aaatgggaat gacacaggtt gttgaaattg gtccagggaa
     2221 ggtactctca ggtttcttga aaaaaataga tagctcatta tcagtatatt ctgttgaaga
     2281 taaaattgga tttaataatc ttaaggaatt aaactagggg aaaatatgca attaaaagat
     2341 aagaacattt ttattacggg ttcaagtaga ggaatcggcc ttgcaattgc ccatcaattt
     2401 gcacaattag gtgctaatat tgttttaaat ggacgttctg aaatatcaga agacttaata
     2461 gctgaatttg ctgattatgg tgtaaaagtt attgctattt caggagatgt ttcttctttt
     2521 gaagatgcta atcgcatgat taaagaagct attgcaagtc taggtagcgt ggatgtgctt
     2581 gttaacaacg ctggtattac taatgataaa ttgatgctta aaatgactgt tgaagatttt
     2641 gagagcgttc ttaaaattaa tttaacaggt gcatttaata tgacacaatc agtcttgaaa
     2701 ccgatgacaa aagcgcgtca aggtgctatt attaatatct ctagcgtcgt tggattaact
     2761 ggaaatgtag ggcaagctaa ttacgcagcc tctaaagcag gtttaattgg ctttacaaaa
     2821 tcagttgcca gagaagtagc agcacgaggc attcgtgtca atgccattgc tccaggcttt
     2881 atcgaatctg atatgacaga tgttatccca gaaaaaatgc aagaagctat tttagctcaa
     2941 attccaatga aacgtattgg aaaaggtaaa gaagtagcgc aggtagcgtc tttcttagca
     3001 gaacaagaat accttacagg acaagttatt gccattgatg gcggtatgac gatgcaataa
     3061 taaagaggaa ataatatgac attacagcga gtagtagtaa caggttacgg tgtaacctca
     3121 cctattggaa acacaccaga agaattttgg aatagcctta aagaaggaaa tgttggtatt
     3181 ggacctatca ctaaatttga ttcaagtgat ttcatggtca aaaatgctgc tgaaattcat
     3241 gattttccat ttgataaata ttttgtcaaa aaagatctga acagatttga tatgtattcc
     3301 ctttatgcct tatatgcttc aagtgaagct attcaacatg ctaatttaaa tttagatgaa
     3361 attgatgcag atcgatttgg tgtcatcgtt gcatcaggta ttggagggat tcaagagatt
     3421 gaagaacaag tgattcgtct tcatgaaaaa gggccaaaac gcgtaaaacc aatgacgctt
     3481 ccaaaagctc taccaaatat ggcagctgga aatgttgcga tgaggttagg cgctcatggt
     3541 gtatgtaaat caattaatac agcttgtgct tcttctaatg atgctattgg agatgctttc
     3601 cgtaatataa aatttggtat tcaagatatt atggtagtcg gtggtgcaga agctgctatt
     3661 acaaaatttg ccattgctgg attccaaagt cttacagcat tatcaacaac tgaagatcca
     3721 tcaagagcat ctattccatt tgataaagat cgcaatggat ttatcatggg agaaggttct
     3781 ggaatgttgg tcttagaaag tttagaacac gctgaaaaac gaggagctac tattcttgca
     3841 gaagttgttg gttatggtaa cacttgtgac gcatatcata tgacttcacc acatccagaa
     3901 ggtttaggag ccactaaagc tattcaactt gcactggctg aagctaatat taaacctgaa
     3961 gaagttaact atgtaaatgc tcatggaact tcaacacctg ctaacgaaaa aggagagagt
     4021 caagctgttg ttgctgcact tgggacagac gttccagttt cttcgactaa atcttttact
     4081 ggtcatcttt tgggtgccgc aggagctgtt gaggcaattg caactattga agcgatacgt
     4141 catagttatg ttccaatgac tgctggaaca acagaattat cagaagatat tacagcgaat
     4201 gttatttttg gtcaagggca agacgctgat attcgttatg ccatttcaaa tacatttggt
     4261 tttggaggac acaatgcagt tctggccttc aaacgctggg aggattaaat tggatattca
     4321 agagattaag gatttaatga ctcaatttga tgagtcaagt ctgagagaat tttcattcaa
     4381 aacaagtgac ggtgagctgt cttttagtaa aaatgaggga aaagctccac ttgtaccaac
     4441 tatgtctcct atgagtcatc aacctgaagc gactccaact atagcaactc ctgtatctaa
     4501 tgaagcaggt gagcaaacga aacaagctac tgaagtagtt tcagaaatcc cagagtcgac
     4561 tgtgactgtt gcagaaggtg acgttgtcga aagtccttta gttggggttg cttatttagc
     4621 ttctggacca gataaaccta actttgtttc agtaggtgat agcgtgaaga agggacaaac
     4681 tttgatgatt attgaggcaa tgaaggtgat gaatgaagtg cctgcccctc atgatggtgt
     4741 agttactgaa attcttgtag ccaatgaaga agttattgaa tttggtaaag gattggtacg
     4801 aatcaaatga ttgatataaa agaaattcgt gaggctttac ctcaccgcta tccaatgtta
     4861 ttagtggatc gtgttttaga ggtatcagaa gatgagatag tcgctatcaa aaatgttagt
     4921 atcaatgagc cttttttcaa tggtcatttt ccagaatatc ctgtaatgcc aggcgtttta
     4981 attatggaag ctttggctca aactgcaggt gttttagaat tatctaaaga agaaaacaaa
     5041 ggaaagcttg ttttttatgc aggtatggat aaagtgaaat ttaaaaagca agttgttcca
     5101 ggagatcaat tagttatgac tgctaagttt gtcaaacgta gaggaactat agccgttgtt
     5161 gaagcaatag cagaagttga tgggaaatta gctgccagtg gaacgttaac atttgcaata
     5221 ggtaattgaa gatagtattg aatttagagg gagagaagca tcaaaaatgt ttaagaaaat
     5281 attgattgct aatcgcggag aaattgcagt acgtatcatt cgtgcagccc gtgaaatggg
     5341 aatttcgaca gttgctatct attcggaggc tgacaaggaa tcattgcaca caattttagc
     5401 agatgaagct atttgtgttg gaccagccaa atcagcagaa tcttatttaa acgttaatgc
     5461 tatattatct gctgctattg tgactggagc ggaagctgtt catccaggtt ttgggttttt
     5521 gagtgaaaat tctaaatttg ctactatgtg tgaagagatg aacttaaaat tcattggtcc
     5581 atctggagaa gttatggata aaatgggtga taaaatcaat gctcgtgcag agatgataaa
     5641 agctgatgtt ccggttatcc caggatcaga tggtcaagtt acaagtgtag aagaagctgt
     5701 atcaattgct gaggaaatcg gctatccatt gatgttaaaa gcctcagccg gtggcggtgg
     5761 aaaaggaatt aggaaggtca aatcagcaga tgaattgaag cctgcttttg agtctgcttc
     5821 acaggaggct ttggctgcgt tcggtaatgg tgctatgtat attgaaaaag ttatataccc
     5881 agctagacat attgaagtac aaatacttgg agatagcttt ggtaagatag ttcacctagg
     5941 agaacgtgat tgttctctgc agcgtaacaa tcagaaggta ttggaagaaa gtccgtctgt
     6001 ggcaataggt aacactctgc gtcaacaaat tggtgaagca gctgttcgtg cagctgaggc
     6061 tgtatcttat gaaaatgcag gaacaattga gtttttatta gatgagaata gcggacagtt
     6121 ttatttcatg gaaatgaata ctcgtgttca agtagaacac cctgttacag aatttgtaac
     6181 gggtgttgac atcgttaaag agcagattcg tatcgctgct ggaattcctt tatcagtttc
     6241 tcaaaatgat attaagttaa ctggtcatgc gattgaatgt cgtattaacg cagagaatcc
     6301 acaatttaat tttgcgccat gtccaggtac cattaatggg ttacatctac ctgctggcgg
     6361 tatgggctta cgtgtcgatt cagcggttta cacaggctac actattcctc cttattatga
     6421 tagtatgatt gcgaaagtaa ttgttcacgg agaaaatcgt tttgatgcac ttatgaaaat
     6481 gcaacgtgcc ttatatgagc ttgaaattga tggtattata acaaatacag aatttcaaat
     6541 ggatctcatt tctgataaaa aagtcttggc tggagattat gatacttcat tcctgatgga
     6601 agacttttta ccaaggtatt atggtaacaa tcaataggag ggaagatggc tttatttagt
     6661 aaaaaggata aatatatccg tattagtcca aacaaagcat tagggtcttc tgataaacgt
     6721 agtcttcctg aggttcctga tgaactcttt gctaagtgtc cttcttgtaa acatatgatt
     6781 tatcagaaag atttaggatt agcaaagatt tgtccagctt gttcttacaa ttttagaatt
     6841 tcagcgcaag aacgtctgtt gctaacagtt gacgaagata gttttgagga attatttaca
     6901 gggattgaaa ctaaggatcc tttgaatttt ccaaactacc gtgaaaaact ggctgctaca
     6961 cgtcaaaaga ctaacttaga tgaagcggtg gtgacaggtt tagctaaaat aaaaggacaa
     7021 actacggcct tagctattat ggattctcat tttataatgg cgtctatggg aacggttgta
     7081 ggcgaaaagt tgacaagatt atttgagcta gcaactgaaa agaaattacc tattgttatt
     7141 tttacagcgt cagggggcgc cagaatgcaa gaaggtatta tgagtttgat gcaaatggca
     7201 aaagtatcgg cagcagttaa acgccattct aaccagggat tgttttattt aacaatttta
     7261 acagacccaa caaccggcgg cgttactgcg agttttgcta tggaaggtga tattattctg
     7321 gctgagccgc aagcgttggt tggttttgct ggtcgtcgtg ttatcgaaac aacggtgaga
     7381 gaagatttac ctgaaggctt tcaaaaggca gagtttttgt tagaacatgg ttttgttgat
     7441 gctattataa atcgaacaga attacgcgac tgcattgctc aactaattgc tttccatgga
     7501 aatgaggttg gtcatgaata atgatgtgac aagaatttta aaagatgcgc gtgatcaagg
     7561 gaggctaact gctcttgact acgctgaatt gatttttgat aattttatgg aacttcatgg
     7621 tgatcgccag ttcgcagatg acaaatcaat tataggaggt ttggggtact tggcagggcg
     7681 tcctgtgacg attgtcggta ttcaaaaagg taagaacctt caagataatt tagaccgtca
     7741 ttttggtcaa ccacatcctg aaggttatcg taaggcttta cgtttaatga aacaagctga
     7801 gaagtttgga agacctgtta taaccttcat caatactgct ggagcatatc ctggggtagg
     7861 tgctgaggag cgaggtcagg gtgaagcaat tgctagaaac ctcttagaaa tgagtgattt
     7921 aaaagtccct attatagcta ttattattgg tgaagggggt tctggaggag cattagcttt
     7981 agctgttgcc gataaggttt ggatgttgga acatactgtt tattctattt taagtcctga
     8041 gggctttgcc tctattttat ggaaagatgg tacaagaaca acagaagcag cacaattaat
     8101 gaagatgact gctggagaac tttaccatat ggaagttgta gacaaagtaa tccctgaaca
     8161 tggttatttt tcaagtgaga ttgtcgatat gattaagaca agtttaattt cagagttaga
     8221 aatgctctca caattatctc ttgaggattt attggagcaa cgttaccaac gttttagaaa
     8281 atattaatag aaaaaggctg agttatttac tcagcctata tttagttttt caaggtatta
     8341 tcatagttat agtttcatat tttgctataa ttgataaaaa ggagatatgt caatgacact
     8401 agcattagcc aatttttaga ttgtagtcta atccaaaatt ttcagaaaga gtcatttcga
     8461 acactttatt ctaagtacca ttatttaggc tctccttaac ttgagactct atctgttatt
     8521 gaagagaaat tttctagaaa aaatagctat tgttagattt tagaaggcta tgaaaagatt
     8581 ggtttttaaa gagttctatt aagtgatgaa gaaagtaaaa cttggttagt tataattgcg
     8641 attagacttt gtttctagca acgagacttc gctatacata ctttaaaaga acttgaaagt
     8701 atgtatagat atataatcgt gaaaacttaa ctattgttta catggagaaa aagaatgtat
     8761 caagaattaa atgattattt agaattccaa tcaatgaagt atattatgcc tgagaaggca
     8821 ggccctttaa aggacgatat gcaaactttt aaggcaaagg gccaacttgc taggaatagc
     8881 tttacagagt tatcaagagc cttagaacaa cgtatggatg gctttaaaat gcaaagagtg
     8941 agtaattggg ctaatcaagc tcaagtaggg cgtccacatt tttgggttta ttatcgaaag
     9001 gatacagacc aattagatga tgtggctgtt gcgctaaggg tttatggtgt aaaagatagt
     9061 tttggggtta gtcttgaggt aagctttgta gagaggcaaa aatctgataa gacactcgag
     9121 aagcaggcta gagtgttatc gattccgatt gcctctccat tgtattttat ggtccaaagg
     9181 caaggtgaaa ctcatcgaga ggaaggcaat gaggaaaatc gtcaacgttt aatgcaagaa
     9241 attaagtcag ggaaggtaag gaaagtattg gtaaaatatg atgttctttt gactgaaaac
     9301 cagagcttag aaaatattct gcaacggttg ttagaaggat ttgagaaagt gctaccttac
     9361 taccaagttt gtcaaactat tagctaagtt catggaaaaa atgagttggt ataccaactc
     9421 attttagagt gcctaatttg gtttaataat atcgatatta cccatatatg gacgaagaac
     9481 ttctgggata gtcacagaac catcttcatt ttgataattt tcgaggattg ctgctacagt
     9541 acgtccaact gcaagtcctg aaccatttaa agtatgaagc aacctaactt tgccatcaac
     9601 ttcgtcacgg taacgaattt gagcacgacg tgcttggaaa tcttcagtat tagaacatga
     9661 agaaatttct cgataagtat tttgagcggg aatccatacc tctaagtcat acgttttagc
     9721 tgctgagaat cccatatctc ctgtacacaa ggtaatgaca cggtatggga gattaagttt
     9781 ttgaagaata ttttcagcat ttgctgtcat tttttctaat tcttggtacg attcctcagg
     9841 cttggcaaat ttcaccattt ctactttatg gaattgatga aggcgaatta atccacgtgt
     9901 gtcacgtcct gcagagcctg cttctgaacg gaatgatgga ctcatggcag taaagtatat
     9961 aggtaattct ttcccatcga taatttcatc acgataatag tttgttaaag gaacttcagc
    10021 tgttgggata agtacgaatg ggctatctgc taattcgaaa gtatcttcct tgaactttgg
    10081 atattgtccc gtaccgaaca ttgaatcgtg gttaaccata taaggaggga tcacttcagt
    10141 gtacccttcc ttagcgtgtt catccagcat gaagctgtag atagcacgct ctaaacgagc
    10201 acctagacct ttgtagaaga ggaagcgaga accagtaact tttgcaccac gttcccaatc
    10261 gagaattcca aggctttccc ctaaatccca gtgagctttg gcctcaaaat caaattcacg
    10321 aggagttccc cagcgacgaa cttcaacatt ttcatcctcg tctgctccaa gcggcacatc
    10381 atcagctggt gtattaggaa gtacggtcac cattgattgt aaattagcat ccacatctgc
    10441 caattccgca tcaattgctt tgatatcagc ggaaagtgtt tgcatagctg tgatttgctc
    10501 gtctgcattc tctttatttc gttttgcttg agcaatggca gcagaagcaa cattacgttg
    10561 cgctttcgcc tcttcagatt tgataagtaa ttcacggcgt tttatatcaa gttctttaag
    10621 ggttgttaat gtttcttggt caacaccacg cgtggccaat tttttagcga cagcatcaaa
    10681 atctgtacga atacgtttta agtctaacat ataggaatct cctttatact aaaaaaaaca
    10741 ccctgtcatg ctggagcgca aaggcgcggt tccatccagc ttcacagaag tgcacttgat
    10801 tgattgatta aattatttcc cataaatgga acacggtaga atttcataaa acaatcttat
    10861 ctgctcgcaa cgaccgcaga ctctctgaaa agaactatta tactacttat ccgttaaggt
    10921 tattatatcc aaaaataagt aaaatgtaaa ccttaacttt ataagcctgc tctttgtttt
    10981 agtgaaatag ctatgctaaa atatgattat ggaaaagtat acaagtatta aacaatcagg
    11041 tcgtcattac ggcttagacc ttttaaggat tatttccatg ttcatgattg ttattacgca
    11101 tgttcttgga aaaggtggtc tgagatcttc ggttgaggga cacgcagact cctattttat
    11161 agtaacgtgg attatccaag tcttagttta tggggccgtt aactgttacg cactaataag
    11221 tggttatgtg ggaattaatt cgcgttatcg ttactcaaaa ttactgtcta tctggacaca
    11281 ggtttttttc tacaccttta caataacagc actttttgca atcacaggtc atgaagtgac
    11341 cttgcttaat tggagagatg cttttttccc tattgtcagt gggcagtatt ggtacattac
    11401 tgcttacttt ggtctattgg tttttatgcc tgttattaat aatggattaa atgccttgac
    11461 tgataaacaa ttaaaacaat tggttttgtt aatgtttatt attttttcaa ttttaccagc
    11521 tgttttaaac aatcgtgttc cagaattttc attgagcaag ggttttgaaa tgacttggct
    11581 actgatttta tatattattg gtgcctattt gaaacgtatt gatttaaata tatttaaaac
    11641 atcatactta cttatagttt atctattatc attggttgct acttatgtca tgaagtttag
    11701 tattggtgat atttggtatt ggtatgtttc gcctacttta actttagggg cggtaagttt
    11761 gtttatatta tttgctagag caagcattaa gccctcggga ttcttaaaga aaataattgt
    11821 tgtattagcc ccttcaacct tgggagttta tctctgtcat ctccacccac taatagtaaa
    11881 atattttgta agagattttg cagagacgtt tgtttatgag tccatttatc tttacccatt
    11941 tttaatttta ggagcaggga ttttaattta tttgttggct tttattgtag aacgtattag
    12001 attacgaata tttaaagcaa tccatttttc taactttttg agatggtttg atgacaagtt
    12061 cccatattaa tattagagtt aaaaaataag atagactata gtctatctta ttttttaaac
    12121 ttgaatattt ttaaaatggt ctggatgagg gtaggaagct taacaacttt atcgtctcca
    12181 atatgtcgtc tcgcatgctt taatatcgtt ccagagtctt tagaggcaaa taaaaatttt
    12241 ccttggtctg taaagacctc aaagtgacga ctaatttttt ttcgactaac attagctcca
    12301 atttgattga ttgatgtcca tggaatttga atatagtcgt tgacattttt atcgttatag
    12361 aattctaatg cctggtcacc aactaaaaat tttccaactt tattccccat acctaaatac
    12421 gaaactcctg ttgtttgaaa ttcaacagtt ttgtttaatg attgtgccat gactaatccc
    12481 tttttaattt atagtagttt gattatatca taatagtggg actctaagtg gatttttggt
    12541 aataaaatat aagagatgga atactattgt aaccatctct tagttttatt acatcacacc
    12601 tgcaaggcga gctagaatac caacgataaa caaaccgatg ataattgtaa ttggtgatac
    12661 tttcttctta agtaaccaca tacagaagaa tgtaagaagt aatccagcta aaccaggaat
    12721 aagtgagtca agttgtcctt gaagtgtttg tggttgcatt ttatcaaggc ttaagccact
    12781 ctgaacttgt cctaggatat catgcaattg ttgtccacta acatggtctt tagggaaatt
    12841 gatgtatgcc ccttctgaca atgtcttttt aggaaggtct acagtaaagt taattgctac
    12901 ccaacgctta acaaggacag caagaatgaa cataccaagg attgaagcac ctttagtaat
    12961 atcttggagg ataccgccag acatatcttt agtaatttct ttacctgatt tgtaacctaa
    13021 ctcttgtgta taccataaga atgacatacg aatgaggttc caaccaacaa agaagatgat
    13081 tggtccgagg atattaccag cagaggcaag tgaagcacca agagcaccta agataggacg
    13141 tacagtaaac cagaatactg gatcaccaat accagcaaga ggtcccatca taccgatttt
    13201 aaccccttga attgctttgt cttcgacagg tgtaccgctt gctttttctt cctcaagagc
    13261 aagggttaca ccgataattg gagcagcaac gtatggatgt gtgttaaaga attccatatg
    13321 gcgctcaagc gcagcagcac gatcttcttt agtagtgtaa agttttttca atgctgggat
    13381 aagagcgtag gcccacccca tattttgcat acgctcatag ttccaagaac cttgaaggaa
    13441 ttgtgaacgc caccaaactt tttgacgatc tgattttgat aatttaattt gttcagtcat
    13501 tgtttagctc ctttctagta gtcctctaag atatcgccga taggatcgtt agaacctgcg
    13561 ttagaagtac ctttgcttcc acccattttt gataaactga tgtagatgag ggcgatagca
    13621 acaccaatag tacccattgc aataagagtt aactggttca aagctgctag agcaaatcca
    13681 agagcaaaga aaggccaaac ttcgcgagta gccatcatat tgataaccat agcgtaacca
    13741 acggcaacaa ccatggcacc accaacttgc ataccaccat ttaaccaatc aggcattgct
    13801 tcaagaatac tttgtactga agaagatgga atagctaaaa gaagcgcagc tgggaatgca
    13861 atacgtaatc cttggagaag gagggcaata aagtgaaaac gttcaattgc tgcgaagttt
    13921 ccttcagaag cagctttgtc accagcgtga acaagtgcag ttgagattgt acgaacaatc
    13981 attgtaagga aaagtccagc aatagctaga ggtacagcag tagagaaggc aaagttgata
    14041 cctttttgag tgaagtcgcc acttttaacc aaaataatag cagcagcaac agaggcaagt
    14101 gcagcgtctg gtgctacggc agctccgata tttgcccaag caagagcaat catttggaga
    14161 ctaccaccaa gaataatacc cgcttcgagg tgaccagtta caagaccgat taaagtacaa
    14221 gcaacaagag gttgatggat ttggaattgg tcgaggatac cttcaagacc agcaaagaag
    14281 gcaacgataa cgaccaaaat cattgagata attgaaatat ccgccatttt gatatccttt
    14341 cgttttcaga gataataagt tattttacgt ttgctttgtt gattaaatca aacaaattct
    14401 ttttagcgtc atttggtact ttacgtacat caaatgatac acctaagtca cgtaattttt
    14461 caaaggcagc aacatcgtct ttatccattg aaagaacgtt gtttaccatt gttttaccag
    14521 ttgagtgagc cattgaacca acgttcaatt cagggatttc cacaccacct tcaactgcac
    14581 ggagtgcatc ttgtacagtt tcaaataaga tcaaggcacg tgtgtttccg aaacgtgggt
    14641 ctttagctac ttcaattaat ttactaattg ggacaacgtt tgctttgact cctccaggag
    14701 cagcttgctt aatcaattgt ttacgaagtt catctttaga aacttcatca gaagcaacga
    14761 taatacggtt tgcttttgat gctggagtcc atgcagttgc aacttgacca tgtaaaagac
    14821 gtgtgtcaat acgagcaaga ttaattttaa gcttgccatc tccaataaca gttccttcag
    14881 ggatagatcc actttgttta acttccgcag gaacatctgc aggaacacct gctacaggag
    14941 tagcttctac aacaggatta agttcttctg gaagggcttt gataccttcc ttagactctt
    15001 taataatatt tgcggcaact tgttcaacac ctgcattagc gtccatcata cgctcagtat
    15061 aggcttggat aagcattggc aaattgagac cagtgatgat agccatttta cgttcaggat
    15121 tttctcccat aacacggctt gcttggttaa atggtgaccc actccaaagg tcagctaaca
    15181 caagcacctc atcatcagca tcaaattgtg cgatagcatt gttgaagtgt ccatacaaat
    15241 catctggtcc ttcatttggc atgaaagtta caacttgaac tttttcttgt tcaccaaaga
    15301 tcattgaacc tgattgatga ataccttcag caaatttacc atggctggca ataataatac
    15361 cgatacccat tcttgttctt cctccttaaa aaattttttc gttgacataa agaagaataa
    15421 tctccttcca atcgtaaaat tggccattct ttttcatttc aacgaaatta tagaacagat
    15481 atattttaaa acgttttcag aaaaaaatca atactatttc taataaaaat taaaaaaaag
    15541 atattttttt cacaaatacc ttttataatg ggggaagaga gtggtaactt tttttgttaa
    15601 taatatagta gaaaacctaa aaaacgattt ctattagaat tctgtttcaa tagagatcgt
    15661 ttttgaaaat actacaataa taatttttct agagttttag caacaccatc ttcttcgttt
    15721 gtccattgga tttgttggtc ggcatatgga agtagagttg gactagcatt tttcatagca
    15781 tatcctgtat gtgcaaaggc cagcatctcg gtgtcattgt gttcatctcc aaaggcaatc
    15841 aaattttctt gtgaaagatt gagtgatttt aataaatgtt tgagagcata agctttgtta
    15901 acacctttag gtgaaaattc gagtatattc aatggaccac cccaagaatc tacttctagt
    15961 tcgtgattaa aatgttgtcg taactcttta gctaactcat acttatcttt atgacgtgtt
    16021 tgcatcaata gagcatttgg attgcgtgtg attttagtaa catctaatgc cattttatca
    16081 gtaatttcat tgacaccgaa taattgtggg ttaattttat cacgattatc catagtaatg
    16141 taaaaatttt tacggtactc gctagcaata aaatcggctt ggatagcatc tgataaattt
    16201 aaggtttcaa ggagtaattt tttatcaatc gttgcactat gctcaaacgc ccactttttt
    16261 tcggggatat gagttaaagc accgttaaaa tttatcattg gtgtttttaa atctaggcgt
    16321 aaataatgag cgagtgccat acggtatggg cgtccggttg taataattac tttgtgtcct
    16381 tgagcttgaa cttttcgaag tgtatcagcg gtgtagtcag aaatagtgtt attattatgt
    16441 aatagagtac cgtctaaatc tacggcgata attttctttg tcatgctgac attatatcaa
    16501 aaatagattt caataaaaag agacagtaga aatatgatat aataactgtg gaaatatgga
    16561 agttggtgga ttatgaaaaa gttaatttgg agtcatcttt ttgccatagt cagtatttta
    16621 gggatatggt attatcatgt aaaaattgat catttgatgg taattctatt ggcatcagtc
    16681 ttgtcgttag agatgggatt cattgcttat tatagtcgta atatttttat tcgtctttta
    16741 gcaagtttag gccttttatt tttttaccca cagagtttag ggatttttct tagtttagtg
    16801 agcttagagt ggtcaacaaa agacatttgg cagtctaatc actttatgca atatatggca
    16861 taccttcttg caattctttt tattatctct tcagctttac taagtttgaa gctattggta
    16921 aatgcatatg atattagtaa aaggtatcaa ttagcaactg ttatacctgt aatgcttttt
    16981 agtagttttg ccttttgctt agaaaaatca ggatcattag gtttagaagg aatcatttta
    17041 aatccgtcag cattttttaa tggaatacag actaatatgg ctaaccttga ttttaaagcc
    17101 ttgtcttgcc tttttttagt acaattatta ctttggggaa tattaatgga agactaacca
    17161 caacagggtt agtcttttag gtttgaagtt tatttaacct taatttaata atatgttgta
    17221 taatgagata ttaattggag gttagaatga aagtaaaact atttgcgata catttatttt
    17281 ttattgttat ttcttttggg attaaatatt atcatttgca gggaccaaat ttgatttgga
    17341 atatgactct agccctaata gcgttagatt ttgcatacct tactagttta tttaagaaaa
    17401 aaattcttat cggtcttttt gcacttgctt ggtttttttt ctatcctaat accttttata
    17461 tgttgacgga tatcattcat atgcattttg tcggagacgt tctttataat aagacaaatt
    17521 taatacttta tatcttgtat gtatctagta tcttgtttgg ttttttatca ggtatcgaaa
    17581 gtttttctgt gattatgcgt aaatttagaa ttagcaatat atttttaaga tggggaatta
    17641 ttggaattgt atcatttgtt tcaagttttg gtattcatat tggtcgttat gcccgtctaa
    17701 attcatggga tatcttaacg aagccacaag tggtaattaa cgaactttta gcggttccga
    17761 gtagggattc attccacttt attttaggat ttacattttt acaggtattg tgtcttattt
    17821 ttatggatta ttctgcaggg aaaaagcttg aaaagtgaat tattttttgt taatatatat
    17881 taatagttcg taaatatacg aacgattaat ttttttaaaa gtgtaaaata acaaaaaata
    17941 aaggagaatc cgattttaaa atggaaaagt tttttaagct aaaggaacac gggacgacaa
    18001 ttcggactga aatcactgct ggtctaacga ctttctttgc aatgtcgtac attttgtttg
    18061 taaatccagc tattttatct cagactggga tgccagcaca aggagttttc ttagcgacga
    18121 ttattggtgc cgttgtagct acatctgtta tggctttcta tgctaacctt ccctatgcac
    18181 aagctcctgg gatgggatta aatgctttct ttacatatac agttgtcttt gcattgggat
    18241 atacatggca agaagcctta gctatggtat ttatttgtgg tttgatttct ctgattatta
    18301 ctttaacgaa agttagaaag atgattattg agtctatccc aacaacttta aaatcagcca
    18361 ttacagcagg aatcggaact tttttagcat acgttgggat taaaaatgct ggttttttga
    18421 aattttcaat tgatcctggg acgtatgatg ttgttggaaa gggtgctgcg aaaggattgg
    18481 ctactattac agcaaattct tcagcaacgc cagggctagt gtcgtttgat aatccagcaa
    18541 ttttattatc tttgattggt ttaagcatta ccatcttctt tattgtaaaa ggtatccgtg
    18601 gagggattat tctatcgata ttaacaacga ccctcttagg tattttaatg ggagtggtta
    18661 agttagatgc tattaattgg gaagcaacta acctctcggc ctcttttaga gatttgaagc
    18721 aagtatttgg agttgcatta ggagagaaag gattaatttc tttattttct aatccttcac
    18781 gtctaccaag tgtattaatg gccattcttg ctttttcact gactgatatt tttgatacca
    18841 ttggaacgct cattggtacc ggagaaaaag ttggtatctt agcaacgact ggtgataatc
    18901 atgaatctaa gagtttagac aaagcactct attcagactt aattgggaca acttttggtg
    18961 ctatttgtgg aacatctaac gttacaacct atgttgagtc agcagcagga atcggagctg
    19021 gtggtcgtac aggattgaca gccttagtag ttgctggttt gtttgctata tctagttttt
    19081 ttagtccact agtatctatc gttccgagtc aagctactgc accaatattg gttattgttg
    19141 gtattatgat gctttcaaat ttaaaagata tcaaatggga tgatatgtca gaagctatcc
    19201 cagctttctt tacttctctt tttatggggt ttacttatag cattacttat ggtattgcag
    19261 ctggtttctt gacctataca ctagctaaag tcatcaaagg acaagcaaaa gatattcatg
    19321 tggtactgtg gattttagat atattattta ttctaaactt tatcagcttg gctattttgt
    19381 aatattatag tttgataaat aagataaaag gaaatcagat ttgtagcctg gtttcctttt
    19441 ttcttaagaa aagtgaagaa atatatgcta tggggctgtc agaatcagca aactgttgga
    19501 agacagagat atttctggta taatatctct atgttttata ctcaaaatga agaagagctt
    19561 attgccctag ggcaaaaact gggtactgtt ttaaagtcag gagatatcgt attattgact
    19621 ggtaatttag gtgcaggcaa aacaactctt acaaaaggta ttgcaaaggg attagacata
    19681 aaacaaatga ttaagagccc aacttacact attgttagag aatatgaagg gcgtgtacca
    19741 ctttaccatt tggatgttta tcgtattgga gatgatcctg attctattga cttagatgac
    19801 ttcttgtttg gtcaaggagt aacagttatt gaatggggag agttgcttag tgataacctt
    19861 atcaataact acttagaaat tgttattacg aggagcaatc aaggacgtca agttcagtta
    19921 gaagcttatg ggcatcgcgc aagagaaatt attgaggcga ttcaagatgt ctaaacaatt
    19981 tgctgttgat tttatagaag cttcgagaga ggaagcatct gcgcttcttg aatttttaaa
    20041 tactgttact gaggaaacag attttatttt gcatacggtg agcaatcaac tatctttgcc
    20101 agaaatggaa acgtttattg aaaatacctt aataacgaaa aattgcattt gtttgattgc
    20161 taaattaaaa aataaagtga tcggcttgat aacaatcatt agtcagtctg atattgaaat
    20221 tgaacacatt ggggatcttt ttattgctgt tcagaaggat tattgggggt atggtattgg
    20281 tcatattctc atggaagaag caatagaatg ggcgtcagat aacgatatta cacgccgctt
    20341 agaactatca gtccaaggac gtaatgaacg tgctattcac ctctaccaaa aattcagttt
    20401 tgaaatagat gggcttcaaa caagaggtat taaaagagaa aatggagaat ttctagacat
    20461 ttacagaatg agtaagttga tagattagga atatacatga aaatttggaa aaaaataacc
    20521 ttaatgtttt ctgcaattat tttaacaaca gtaattgcat tgggagtcta tgttgcctca
    20581 gcttataatt tttcgactaa tgaattgtct aagactttta aagattttaa gctagctaaa
    20641 tcaaaaagtc atgctattga agaaacaaag ccgttttcaa tactattaat gggtgtggac
    20701 acaggttcag agcatcgaaa atctaagtgg tcaggaaata gcgattctat gatcttagtc
    20761 actataaatc ctaaaactaa taaaacaacg atgacaagct tagaacgtga cgtattgatt
    20821 aaattgagtg gtcccaaaaa taatggacag actggcgtag aagcaaagct aaatgcagcc
    20881 tatgcttctg gtggtgcgga aatggcattg atgactgttc aagacttatt agatattaat
    20941 gttgattact ttatgcaaat taatatgcaa ggattagttg atttggtcaa tgctgttggt
    21001 ggtataacag taactaataa atttgacttt ccaatatcaa ttgctgccaa tgaaccagag
    21061 tacaaggctg ttgttgaacc agggacacat aaaataaatg gagaacaagc acttgtttat
    21121 tctcgtatgc gctatgatga tccagaggga gattatgggc gtcaaaaaag acaacgtgaa
    21181 gtaattcaaa aagtccttaa aaaaatattg gcgttaaata gtattagttc atacaaaaaa
    21241 attctttccg cagtaagtaa taacatgcaa actaatattg agatatcatc aaaaacgatt
    21301 cctaatttgt tagcttataa agattcattg gaacatatta aatcttatca gttgaagggt
    21361 gaagacgcta ctctatcaga tggtggctct tatcaaattt taactaagaa acatctactt
    21421 gcagttcaaa atagaattaa gaaagagctg gataaaaagc gtagtaaaac tctgaagaca
    21481 agcgcgattc tatatgaaga ttactatggt actactgcta gtaatgattc ttctacttat
    21541 tcatcaacac aagagaataa ttataataca acaccttatt cagaagcacc accaagttac
    21601 agtggtaata ctacttatag ttctgagact aatcaaacaa ctcatcaaag ttactataat
    21661 agtagcactc ctgctagtaa ctatagcagt aacactaaca caggtcaggc tgattcaagt
    21721 ggaagtgtta ataattataa cggggctgca acgcctaatc caaacacagg aacgcaacca
    21781 gtaccaagtc aaactaatcc ataaaatata aaaaagagca ttatttgctc tttttatatt
    21841 tagagtagat atttttatac cgtcggctta ttcgtctttc tcttgatatt tagccatgcg
    21901 ttgattaatt tcttctaaac gaggttgaat atctttatta aacacttgga atttatgatt
    21961 gagttcttga gaaattaatc tgattttttc tttttcctga ctaataatag caagatcatt
    22021 tttgatacgt tctaatttta atttagctga atctgtctca ttttttgtat tttgatagga
    22081 atccttgata gtttttctat ttttgtaaag ttcaaacgct cctaaactaa ctaaacctag
    22141 tgcaattaaa cgacctattt tcataattga atctcttttc taatttcttt ggctaatttt
    22201 gcaagtgcct caaagtcggg ttcatgagta gtgtagtgaa ttttgatacc gtctgattca
    22261 tcaaagcggg gaacaaggtg gacgtgtgca tgaaaaacag tttgtccagc aatttcctca
    22321 ttgttgttaa taatattcat tcctttggct tttgtagcag cttggacagc acgcgccact
    22381 ttaggcaagc gttcaaaggt tatctgtgca gttttttcat ccatctctaa cacatttcga
    22441 acatgttttt taggaataag aagggtgtgc ccagtcgtag tttgtgtaat gtcgagaaaa
    22501 gctaatactt catcatcttc ataaactttt gatgaagaga tttctccact gatgatttta
    22561 caaaaaatac aattatccat aactaattcc tttcatgttg aaaaatttat tgggtagcta
    22621 ttaaggaatg gtaaattttt atcgtttgat aggtcataag tcgtacaggt ggataaaagt
    22681 agcctgtatt gattgtgata tctatattta ccatcatact tatcttagca tgacctaaag
    22741 ggtattgcaa tttttaatga tgtttattat tgagtaagta catttctaga attttggaag
    22801 tatctaaaat aaatttaaat
//
LOCUS       DATROQ010000028        21727 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.028, whole genome shotgun sequence.
ACCESSION   DATROQ010000028 DATROQ010000000
VERSION     DATROQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21727
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.028"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(41..607)
                     /locus_tag="VBC94_000942"
     CDS             complement(41..607)
                     /locus_tag="VBC94_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000596040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6979221.1"
                     /translation="MIKRPIHLSHDFLAEVIDKEAITLDATMGNGNDTVFLAKSSKKV
                     YAFDIQEEAIAKTKAKLTEQGISNAELILDGHENLEQYVHTPLRAAIFNLGYLPSADK
                     TVITKPHTTIKAIKNVLDILEVGGRLSLMVYYGHDGGKSERDAVIAFVEQLPQNNFAT
                     MLYQPLNQVNTPPFLIMVEKLQSYENEV"
     gene            complement(600..1535)
                     /locus_tag="VBC94_000943"
     CDS             complement(600..1535)
                     /locus_tag="VBC94_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747785.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEO6979222.1"
                     /translation="MKKRYRAINDYYRELFGEKIFKLPIDAGFDCPNRDGTVARGGCT
                     FCTVSGSGDAIVAPEAPIREQFYKEIDFMHRKWPEVNKYLVYFQNFTNTHAKLEIIKE
                     RYEQAINEPGVIGINIGTRPDCLPDETIYYLAELSERMHVTLELGLQTTYEATSALIN
                     RAHSYDLYKKTVKRVRELAPKVEIVSHLINGLPGETHDMMVENVRRCVTDNDIQGIKL
                     HLLHLMINTRMQRDYHEGRLRLLSQEDYISIICDQLEIIPKHIVIHRITGDAPRHMLI
                     GPMWSLNKWEVLNAIDKEMEKRQSYQGCKVEVKHD"
     gene            complement(1628..2269)
                     /locus_tag="VBC94_000944"
     CDS             complement(1628..2269)
                     /locus_tag="VBC94_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6979223.1"
                     /translation="MLSRQNSKLIQAFIAIILFFSLGLVIKYWPDTVIAFDQTIQESV
                     RGQLPNLSTRFFKLITVVGNTVSQIAIAIMSVTFCYLKKWYPQARFIAVNAIISGICI
                     LSLKLIFQRVRPTLTHLVFAGGYSFPSGHSMGTFMIFGSSIILLQYYMPKSIWKLLCQ
                     GTLGLLIFLIGLSRIYLGVHFPTDVLAGFILAYGILNLTAYIFLSKDWLKKVK"
     gene            complement(2250..2834)
                     /locus_tag="VBC94_000945"
     CDS             complement(2250..2834)
                     /locus_tag="VBC94_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6979224.1"
                     /translation="MTNTRKMVTIAILSALSFVLMMVSFPLIPGAEFLKVDFSILPML
                     VAFILFDLKSSYGVLLLRSLLKVILANRGPETFIGLPMNMVALALFLASFAIFWKNRE
                     SAKDFIKASLFGTVSLTVSMVALNYVFAIPLYAIFANFDIRTFIGVGNYLLTMVIPFN
                     IVEGILISIVFYLTYVACLPILERYKKTNVISSK"
     regulatory      complement(2904..3049)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(3118..3717)
                     /locus_tag="VBC94_000946"
     CDS             complement(3118..3717)
                     /locus_tag="VBC94_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEO6979225.1"
                     /translation="MIRRYSANFLAILGIILVSSGIYWGWYNINQAHQADLTSQHIVK
                     VLDKSITHQVKGSENGELPVKKLDKTDYLGTLDIPNLKLHLPVAANYSFEQLSKTPTR
                     YYGSYLTNNMVICAHNFPYHFDALKNVDMGTDVYFTTTTGQIYHYKISNREIIEPTAI
                     EKVYKTATSDNDWDLSLFTCTKAGVARVLVRCQLIDVKN"
     gene            complement(3710..4381)
                     /locus_tag="VBC94_000947"
     CDS             complement(3710..4381)
                     /locus_tag="VBC94_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979226.1"
                     /translation="MIYKKILKITLLLLFSLSTQLVSADTNDQMKTGSITIQNKYNNQ
                     GIAGGNLLVYQVAQAKDVDGNQVFTLTTPFQGIGIKDDDLTQVNLDSNQAKYVNLLTK
                     AVHKTQPLQTFDNLPAEGIVANNLPQGIYLFIQTKTAQGYELMSPFILSIPKDGKYDI
                     TAFEKMSPLNAKPKKEETITPTVTHQTKGKLPFTGQVWWPIPILIMSGLLCLIIALKW
                     RRRRD"
     gene            complement(4371..5246)
                     /locus_tag="VBC94_000948"
     CDS             complement(4371..5246)
                     /locus_tag="VBC94_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEO6979227.1"
                     /translation="MKKIRQHLVSILLVTIIAVGVGLMAYPSLANYWNSFHQSRAIMD
                     YQDRVTHMDENDYKKIINRAKEYNKQFKTSGMKWHMTSQERLDYNSQLAIDKTGNMGY
                     ISIPKINIKLPLYHGTSEKVLQTSIGHLEGSSLPIGGDSTHSILSGHRGLPSSRLFSD
                     LDKLKVGDHWTVSILNETYTYQVDQIRTVKPDDLRDLQIVKGKDYQTLVTCTPYGVNT
                     HRLLVRGHRVPNDNGNALVVAEAIQIEPIYIAPFIAIFLTLILLLISLEVTRRARQRK
                     KILKQAMRKEENNDL"
     gene            complement(5284..6792)
                     /locus_tag="VBC94_000949"
     CDS             complement(5284..6792)
                     /locus_tag="VBC94_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO6979228.1"
                     /translation="MKKKMIQSLLVASLAFGMAVSPVTPIAFAAETGTITVQDTQKGA
                     TYKAYKVFDAEIDNANVSDSNKDGASYLIPQGKEAEYKASTDFNSLFTTTTNGGRTYV
                     TKKDTASANEIATWAKSISANTTPVSTVTESNNDGTEVINVSQYGYYYVSSTVNNGAV
                     IMVTSVTPNATIHEKNTDATWGDGGGKTVDQKTYSVGDTVKYTITYKNAVNYHGTEKV
                     YQYVIKDTMPSASVVDLNEGSYEVTITDGSGNITTLTQGSEKATGKYNLLEENNNFTI
                     TIPWAATNTPTGNTQNGANDDFFYKGINTITVTYTGVLKSGAKPGSADLPENTNIATI
                     NPNTSNDDPGQKVTVRDGQITIKKIDGSTKASLQGAIFVLKNATGQFLNFNDTNNVEW
                     GTEANATEYTTGADGIITITGLKEGTYYLVEKKAPLGYNLLDNSQKVILGDGATDTTN
                     SDNLLVNPTVENNKGTELPSTGGIGTTIFYIIGAILVIGAGIVLVARRRLRS"
     gene            complement(6833..11137)
                     /locus_tag="VBC94_000950"
     CDS             complement(6833..11137)
                     /locus_tag="VBC94_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HEO6979229.1"
                     /translation="MLKKCQTFIIESLKKKKHPKEWKIIMWSLMILTTFLTTYFLILP
                     AITVEETKTDDVGITLENKNSSQVTSSTSSSQSSVEQSKPQTPASSVTETSSSEEAAY
                     REEPLMFRGADYTVTVTLTKEAKIPKNADLKVTELKDNSATFKDYKKKALTEVAKQDS
                     EIKNFKLYDITIESNGKEAEPQAPVKVEVNYDKPLEASDENLKVVHFKDDGQTEVLKS
                     KDTAETKNTSSDVAFKTDSFSIYAIVQEDNTEVPRLTYHFQNNDGTDYDFLTASGMQV
                     HHQIIKDGESLGEVGIPTIKAGEHFNGWYTYDPTTGKYGDPVKFGEPITVTETKEICV
                     RPFMSKVATVTLYDDSAGKSILERYQVPLDSSGNGTADLSSFKVSPPTSTLLFVGWSK
                     TQNGAPLSESEIQALPVSSDISLYPVFKESYGVEFNTGDLSTGVTYIAPRRVLTGQPA
                     STIKPNDPTRPGYTFAGWYTAASGGAAFDFNQVLTKDTTLYAHWSPAQTTYTINYWQQ
                     SATDNKNATDAQKTYEYAGQVTRSGLSLSNQTLTQQDINDKLPTGFKVNNTRTETSVM
                     IKDDGSSVVNVYYDRKLITIKFAKYGGYSLPEYYYSYNWSSDADTYTGLYGTTLAANG
                     YQWKTGAWGYLANVGNNQVGTYGMSYLGEFILPNDTVDSDVIKLFPKGNIVQTYRFFK
                     QGLDGTYSLADTGGGAGADEFTFTEKYLGFNVKYYQRLYPDNYLFDQYASQTSAGVKV
                     PISDEYYDRYGAYHKDYLNLVVWYERNSYKIKYLDPLDNTELPNFPVKDVLYEQNLSS
                     YAPDTTTVQPKPSRPGYVWDGKWYKDQAQTQVFDFNTTMPPHDVKVYAGWQKVTYRVN
                     IDPNGGRLSKTDDTYLDLHYGDRIPDYTDITRDYIQDPSGTYYYKYDSRDKDPDSTKD
                     AYYTTDTSLSNVDTTTKYKYVKDAYKLVGWYYVNPDGSIRPYNFSGAVTQDINLRAIW
                     RKAGDYHIIYSNDAVGTDGKPALDASGQQLQTSNEPTDPDSYDDGSHSALLRRPTMPD
                     GYRFRGWWYNGKIYNPYDSIDIDAHLADANKNITIKPVIIPVGDIKLEDTSIKYNGNG
                     GTRVENGNVVTQVETPRMELNSTTTIPENQYFTRTGYNLIGWHHDKDLADTGRVEFTA
                     GQSIGIDNNPDATNTLYAVWQPKEYTVRVSKTVVGLDEDKTKDFLFNPSETLQQENFP
                     LRDGQTKEFKVPYGTSISIDEQAYDEFKVSESITEKNLATGEADKTYDATGLQSLTVS
                     GDVDISFTNTRIKQKVRLQKVNVENDNNFLAGAVFDIYESDANGNKASHPMYSGLVTN
                     DKGLLLVDANNYLSLPVGKYYLTETKAPPGYLLPKNDMSVLVISTGVTFEQNGNNATP
                     IKENLVDGSTVYTFKITNSKGTELPSTGGIGTHIYILVGLALALPSGLILYYRKKI"
     gene            complement(11157..11726)
                     /gene="lepB"
                     /locus_tag="VBC94_000951"
     CDS             complement(11157..11726)
                     /gene="lepB"
                     /locus_tag="VBC94_000951"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6979230.1"
                     /translation="MKRQISSDKLSQELDRVTYQKHFWSVIKNTIYILMAVASIAILI
                     AVLWLPVLRIYGHSMNKTLSAGDVVFTVKGSNFKTGDVVAFYYNNKVLVKRVIAESGD
                     WVNIDSQGDVYVNQHKLKEPYVIHKALGNSNIKYPYQVPDKKIFVLGDNRKTSIDSRS
                     TSVGDVSEEQIVGKISFRIWPLGKISSIN"
     gene            complement(11710..11982)
                     /locus_tag="VBC94_000952"
     CDS             complement(11710..11982)
                     /locus_tag="VBC94_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979231.1"
                     /translation="MEDLKKLKRKDLLEIMLEQQLLIEKQEKEIQQLKEALDDKKIKI
                     SKAGSIAEASLMLSGIFETAQLAADTYLENVKNLVDKRGVDNEKTD"
     gene            complement(12264..13412)
                     /locus_tag="VBC94_000953"
     CDS             complement(12264..13412)
                     /locus_tag="VBC94_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO6979232.1"
                     /translation="MENKVKTVAVFSGYYLPFLGGIERYTDKMTADLVKRGYRVVIVT
                     TNHDDLPIIDEDKGRKIYRLPTKNIVKQRYPIINKNREYNTLMKHVSDENIDFVICNT
                     RFQLTTLEGLSFAKKHRLPSIVLDHGSSHFSVNNRFLDFFGAIYEHLLTARVKHYRPD
                     FYAVSKRSVEWLKHFNIEAKGVIYNSVSESLGSDFADTAYLEKSADDIFITYAGRIIK
                     EKGIELLLEAFSMSQYSENVYLQIAGDGPELAHLKEKYQSKQINFLGKLNFEQTMSLM
                     AQTDIFVYPSMYPEGLPTSILEAGLLSSAIIATDRGGTVEVIDSPELGIIMEENTQSL
                     HESLDLLVKDKALREKLQQNIAKRIKEHFTWEKTVEKLDYIIQKELNL"
     gene            complement(13402..14250)
                     /locus_tag="VBC94_000954"
     CDS             complement(13402..14250)
                     /locus_tag="VBC94_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6979233.1"
                     /translation="MKYLAGIVTFNPNIERLDQNIRAIYPQVSHIYIVDNGSKNKEEI
                     SQLVADYNEEGHLTVDYLTENKGIAYALNCIGQFAVAQEFDWFLTLDQDSVVLGDLID
                     NYENYLLLPKVGMLSCLYQDMNREDLVMQEFDYKEIEECITSAALMKTSVFEETSGFA
                     EEMFIDFVDSEMNYRLSEMGYKTYQVNFIGLLHEIGHSSRVKKFGHVFHVLNHSPFRK
                     YYMIRNAIYIIKKYGKKKRHKYLVFMRNEFVRVLVAEEQKSKKLVAMIKGLKDGLLMK
                     VDKYGK"
     gene            complement(14247..15671)
                     /locus_tag="VBC94_000955"
     CDS             complement(14247..15671)
                     /locus_tag="VBC94_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEO6979234.1"
                     /translation="MKLLKNMFYNTSYQLLTLLLPLVTVPYVSRVLSPQGIGINAYTS
                     SIVMYFTLFGALGISLYGNREIAFVQSNKYKRSKIFWELVVLKLASVSIATLLFFGFV
                     LLTNEWQLFYLIQGINLLATATDISWYFIGVEDFKIIVIRNTIVKLITVVLTFLVVKT
                     PDDLALYMFLIAFASLLGNLTVWHHLKHEIIKIPFSRLDILIHLRPTLMLFLPQITMQ
                     IYLSLNKSMLGAMDSVVSAGYFDQSDKIIRILFTIVSAIGGVFLPRLSSLFSSGKEKQ
                     AKALLLKLVDLSNAISMLMIAGVVGVSSTFAVFFFGKGYEAVGPLMAVESLMIICISY
                     GNALGTQYLLASRRTKAYTMSAVIGLVANVVLNILLIPILGAMGAIISTVITEFIVSL
                     YQAISLRDVFTFKELTRGMLRYLIAATLSGAVLYYINTQMSVSLVNYVIQSLVAVTIY
                     VGIVFITKAPVIQLFRDFRKEHRT"
     gene            complement(15671..17035)
                     /locus_tag="VBC94_000956"
     CDS             complement(15671..17035)
                     /locus_tag="VBC94_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEO6979235.1"
                     /translation="MNLKKVRLFEKFFLDKNLHKTYFILGLTITIVLSLLMPFFNEPD
                     GQYHLAVSGRISNNIIDTSYYGEYNIDSGMRRQSTSYRDGTRFEKYYLNKANFVDGNH
                     LPREINYSKHNFVFWGHLVPAVGLFLGKFVYPSMGVMITVARLFSSFVSITLVALIIR
                     YVKKGKLIYFAIFLSPVVLNSFASLSYDSTGYIVFGAFLSLLINLLVDKSLTKNRSIA
                     LIGLILLVIVACKQNYWLFLLLVIPVLLQVNNPKLMGYNQKLSHLFQGLKSKKLFWLC
                     LTFLSLAIVYVLTARHGGVLAVLRRYVMTFGYNYAGINILSNDITSWLASPYPSQNFI
                     PTWVSSVWYLMVFLVWFSEEKFVKSKNMGFLFLASFFVGILGVFFIMLEYRGAPTSYI
                     EGVQGRYFTVTLLLVQVFASSIPARLNEAGRKVVPYALTGLVIVSNALLLFDTVISLI
                     MRRN"
     gene            complement(17037..17984)
                     /locus_tag="VBC94_000957"
     CDS             complement(17037..17984)
                     /locus_tag="VBC94_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6979236.1"
                     /translation="MVKTAVLMATYNGQGFIHDQLDSIRNQTLRPDYVLMRDDGSTDD
                     TVKVVEDYIKEHRLDGWSITSNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDTWYH
                     HKNKMQVDIMEERQDINLLSADIDIKKLSEEATIPNNFKFNGEETISKYPLDFSYHNY
                     RQGWTFCLRKSFVDQVMTFYSDNLILSHDNLFAGVSGVIGSGYNLNRSVGEHKRHAEN
                     ASGNILNMSSSYERHLQELYFVLSYYIILVPFLEKSSSSQLREAKAYLDFNQQRYKNA
                     QNKDFVAILKQMMTKKRFYDSFSNRIRDLVFLLKDIKRS"
     gene            complement(17993..18928)
                     /locus_tag="VBC94_000958"
     CDS             complement(17993..18928)
                     /locus_tag="VBC94_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6979237.1"
                     /translation="MVKTAVLMATYNGEKFISEQLDSIRQQTLKPDYVLLRDDCSTDE
                     TVNVVNNYIAKHELEGWKIVKNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDIWYL
                     DKNERQFAIMSDNPQIEVLSADVDIKTMSTEASVPHFLTFSSSDRISQYPKVYDYQTF
                     RPGWTIAMKRDFAQAIADNWTFGANISHDNLIETTASIIGNGYNLNESVGVHIRYGGN
                     ASGNSKFVLNWKSPKTQHITGLKTFVDFYETMQKVVNKYQNDVALNQRLAFEKERLEA
                     SKLSLIHNSAFVIKNWRLYNSNSGRMRDLLFAFKK"
     gene            complement(18930..19988)
                     /locus_tag="VBC94_000959"
     CDS             complement(18930..19988)
                     /locus_tag="VBC94_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEO6979238.1"
                     /translation="MMIKFSNKIVQEDLALISNQNYINWERIANKGVLISGSNSMLAS
                     YMVFLLAYLNETRNYQTQIIATARNIEKARDKFSDLVGKDYFTLIPYDVEERLEYDGK
                     VDYIIHAASNASPTAILSNPVSIIKANTIGTLNLLDFAKEKTIENFLFLSTREVYGTS
                     IKEVIDEEAYGGFDILATRACYPESKRMAETLLQSYYDQYKVPFTIARIAHSFGPGME
                     LGNDGRIMNDLLSNVIDGKDIVLKSSGTAERAFCYLADAVSGLFTILLNGEVGQAYNV
                     ANEDQPIMIKDLAQKLVDLFSDKNISVVFDIPKTMSAGYSKMGRTRLTMAKLEALGWK
                     REVSLESGILKTVQAFEE"
     gene            complement(19988..20710)
                     /locus_tag="VBC94_000960"
     CDS             complement(19988..20710)
                     /locus_tag="VBC94_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEO6979239.1"
                     /translation="MMNIGVIFAGGVGRRMNTKGKPKQFLEVHGKPIIVHTIDIFQNT
                     EAIDAVVVVCVSDWLDYMNNLVERFNLTKVKAVVAGGETGQMSIFKGLEAAEQLATDD
                     AVVLIHDGVRPLINEEVINANIQSVKETGSAVTSVRAKETVVLVNDSSKISEVVDRTR
                     SFIAKAPQSFYLSDILSVERDAISKGITDAIDSSTLMGMYNRELTIVEGPYENIKITT
                     PDDFYMFKALYDARENEQIYGM"
     gene            complement(20710..21534)
                     /locus_tag="VBC94_000961"
     CDS             complement(20710..21534)
                     /locus_tag="VBC94_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEO6979240.1"
                     /translation="MKEMTVSEIREVQLEMLAYIDKVARDNKIEYSLGGGSLLGAMRH
                     KGFIPWDDDIDLMLERSQYERLMKALADANNSDFKLLHHSVEKNLWPFAKLYHTKSMY
                     LSKTDRIHPWTGIFIDIFPLDRLPESAEERQRFFKKVHSAAANLMCTTYPNFASGSRK
                     LYANARLILGLPRFIAYHGQAKKRAEIVDQVMETYNNQEVPYMGYTDSRYRLKEYFPR
                     EIFSEYEDVMFENIKTRKIKNEHAYLNQLYGGSYMELPPESKRESHSYYTWYWKEG"
BASE COUNT         6927 a         4165 c         3351 g         7284 t
ORIGIN      
        1 tttatagcaa ttatcatttt tgctaattgc ctaaagatag ttacacctca ttttcatatg
       61 attgtaactt ttctaccata attaaaaagg ggggcgtatt aacttgattg agtggttgat
      121 aaagcattgt agcgaaatta ttctgtggta attgttccac gaatgctatc accgcatctc
      181 tctcactttt tccaccatca tgaccataat aaaccataag tgatagacgt ccacctactt
      241 ctaaaatgtc taaaacgttt ttgattgcct tgatagttgt gtgtggtttt gtaataactg
      301 ttttatccgc tgagggtaag tatcccagat taaatatagc cgctcttaaa ggtgtatgaa
      361 catactgttc caaattttca tgcccatcca agataagttc agcgttagag atcccttgct
      421 ccgtgagctt tgctttcgtt tttgctattg cttcttcttg aatatcaaat gcataaactt
      481 ttttagaact tttcgctaaa aaaacagtat cattcccatt tcccatagtt gcatcaagcg
      541 ttattgcttc tttatcaata acttctgcta aaaaatcatg agaaagatga attggtcttt
      601 taatcatgtt ttacctcaac tttacatcct tgatatgact gtcttttctc catttctttg
      661 tcaatggcat taagcacttc ccatttatta aggctccaca tcggtccaat taacatatga
      721 cgtggcgcat caccagtaat tcggtgaata acaatatgtt ttggaataat ctccagttgg
      781 tcacaaataa tcgaaatata gtcttcttga cttaacaaac gtaaacgacc ttcatggtaa
      841 tctctctgca ttcttgtatt aatcataaga tgcagaaggt gtaattttat accctgaata
      901 tcattatccg taacacatcg acgaacattt tccaccatca tatcatgtgt ctcccctggg
      961 agaccattta ttaggtgaga aacgatttct actttaggag ctaattctcg tactctctta
     1021 acagtttttt tgtaaaggtc atatgaatgt gctctattta ttaatgcaga agttgcttca
     1081 taagttgtct gaaggcctaa ttctaaagtc acatgcattc tttcagaaag ttcagcgaga
     1141 tagtatatag tttcatcagg taagcaatcc ggccttgttc cgatgttgat cccgataact
     1201 cctggctcat taatagcctg ttcgtaacgc tctttaatta tctctaactt agcatgggta
     1261 ttggtaaaat tttgaaaata gactaagtat ttattaacct caggccactt tctatgcatg
     1321 aaatcaattt ctttatagaa ttgttcacga ataggagctt ctggagcaac tatagcatcc
     1381 cctgaaccag aaactgtgca aaaagtgcac cctcctctag caactgttcc atctctgtta
     1441 ggacagtcaa aaccagcatc tataggtaat ttaaatattt tttctccaaa gagttctcga
     1501 taataatcat taatcgcacg ataacgtttt ttcataggat aattgtatca caattttaac
     1561 taaaataacc tcactactac aataaaacta aaaaagattg gaacgtcagt tagtcccaat
     1621 cttttattta cttcactttc tttaaccaat ccttggataa aaagatatac gcagttagat
     1681 tcaaaatacc ataagcaagt ataaaaccag ctaaaacatc tgtcggaaaa tgaaccccta
     1741 ggtaaatacg agataaccca attaaaaaaa tgagcaaacc caatgtacct tggcacaaca
     1801 gtttccatat actcttaggc atatagtact gcaataaaat aatactactc ccaaatatca
     1861 taaatgttcc catcgagtgc ccactgggaa acgaatagcc acctgcaaat actaaatggg
     1921 ttaaagttgg tctcactctt tgaaaaataa gttttaaaga aagtatacat ataccagaga
     1981 taatagcatt tactgcgata aatctagctt gaggatacca cttcttaagg taacagaaag
     2041 tgacgctcat aatcgcaata gctatctggc ttacagtatt accaaccaca gtgattaact
     2101 taaaaaatct tgtagaaaga tttggcaact gtcctctaac actttcttga atagtttggt
     2161 caaatgcgat tacagtgtcg ggccaatatt tgatgaccaa tcctaaactg aaaaataaga
     2221 taatagcaat aaatgcttga ataagtttac tattttgacg agataacatt agtcttttta
     2281 tatctttcta atattggcaa acaagccacg taagttagat agaaaacaat cgaaattaaa
     2341 attccctcaa cgatattaaa tggaataacc attgttaaaa ggtaattgcc tacaccaata
     2401 aatgttctga tatcaaagtt agcaaatata gcatacaaag gaatcgcaaa gacatagttg
     2461 agagctacca tagatacagt caagctaact gtaccaaata aactagcttt aataaaatct
     2521 tttgcactct ctctattttt ccagaaaata gcgaaacttg ctaaaaatag agctagagca
     2581 accatattca tcggtaaacc gataaaggtt tctggaccac gattagcaag tataactttt
     2641 aaaagtgatc ttaataagag tacaccataa cttgatttca aatcaaataa aataaaagca
     2701 actaacatcg gaaggattga aaaatcaacc tttaaaaatt ctgctcctgg tattaatgga
     2761 aatgaaacca tcatcaatac aaaagataag gcagaaagaa tggcgattgt caccatttta
     2821 cgtgtatttg tcataaaaaa attcctccaa tttaaataaa ttgaaagaag ctccaaaggt
     2881 aagcgtaggt acgcgaaaaa aacctttgtc ttctcccatc cagactttac tgtcggttgt
     2941 ggaatctcac cacatcagct ttcgctcgcg gactgatgct tcacaactga caaataagtt
     3001 ggaagcgatt accgccggtc gggaattaca ccctgccctg aagacaccta tagcataaca
     3061 aaaaaaactt gcaattgcaa gttttttaat cactaattag tagtagattg tataatatta
     3121 atttttaaca tcaattaatt gacagcgcac taatactcta gctactcctg cctttgtaca
     3181 agtaaacaag cttaagtccc aatcattgtc tgatgtggca gttttataaa ctttttcaat
     3241 cgctgttggt tcaataattt ctctattact gattttgtag tgatagattt gccctgttgt
     3301 agttgtaaaa taaacatccg ttcccatatc tacatttttt aaagcatcaa aatgataagg
     3361 aaaattatgc gcacaaatca ccatgttatt agttaaataa gaaccataat accttgtagg
     3421 cgtcttagac agttgttcaa aactataatt agcagctacc ggtaaatgca gttttaagtt
     3481 cggaatatcc agagttccca agtaatctgt tttatccaac ttttttacag gtaattctcc
     3541 attttctgaa ccctttactt gatgcgtaat agatttatca agcaccttga caatatgctg
     3601 agaagttaaa tcagcttgat gcgcctgatt aatattatac caaccccaat agattccaga
     3661 acttaccaga ataattccga gtatagctaa aaaatttgct gaatatcttc taatcacgtc
     3721 ttcttctcca ttttaaagct attattaaac acagaagtcc tgacataatt agtataggta
     3781 ttggccacca tacttgtcca gtaaacggaa gctttccctt tgtctgatgt gttactgtag
     3841 gagtaattgt ctcttctttt ttaggtttag catttaaagg gctcattttc tcaaatgctg
     3901 taatatcgta cttcccatcc ttaggtattg atagtataaa gggagacatt agttcataac
     3961 cttgagccgt tttagtctga ataaatagat aaatcccttg aggaagattg ttcgcaacaa
     4021 taccttcagc cggtaaatta tcaaacgttt gtaaaggttg agttttatga acagcttttg
     4081 ttagtagatt gacgtatttg gcttggttac tatcaaggtt tacttgtgtt agatcatcgt
     4141 cttttattcc aataccttga aatggggtag ttagagtaaa aacttgatta ccatcaacat
     4201 ctttagcttg tgctacttgg taaacaagta aattaccgcc agcgatacct tgattattat
     4261 acttattttg tatagtaata gaacccgttt tcatctgatc attggtatca gcagacacaa
     4321 gttgagtact tagactaaat aataagagaa gagttatctt taggatcttt ttataaatca
     4381 ttgttctctt cctttctcat tgcttgtttt aaaattttct tacgttgacg tgctctccta
     4441 gttacttcta aagagattaa aagtaaaatc aaagtaagga aaatagcgat aaatggtgcg
     4501 atataaatag gctctatttg tattgcctct gctactacca aagcgttacc attatcgttt
     4561 ggtacacgat gtcctctcac tagtaaccga tgggtattaa cgccatatgg tgtacacgtc
     4621 accaaagttt ggtagtcttt acctttaaca atttgtaaat ccctcaaatc atccggttta
     4681 actgttctga tttgatccac ttgataagta tatgtttcat ttaagatact gactgtccag
     4741 tggtctccaa cttttaactt atccaaatca gaaaaaagcc ttgaagaggg taaacctcta
     4801 tgtcctgata aaatagaatg agttgagtct cctccaattg gaagactact tccttctaaa
     4861 tgaccaatag aagtttgaag cactttttca cttgtaccat gataaagtgg taattttatg
     4921 tttatctttg gaattgaaat ataacccata ttacccgttt tatcgatagc cagttgtgaa
     4981 ttataatcca aacgctcttg gctagtcatg tgccacttca ttcctgaagt tttaaattgc
     5041 ttattatatt ctttggctcg gttaataatt tttttataat cgttttcatc catatgcgtt
     5101 acgcggtctt ggtaatccat aatcgctcga gattggtgaa atgaattcca ataattagca
     5161 agtgaaggat aagccattaa gcctaccccc actgcaatta tagtgacaag caaaatggat
     5221 actaaatgtt gtcttatttt tttcatatat tttaaatctg taccactttg ctagcccatc
     5281 ttattaagaa cgtaaacgac gacgagcaac aagcacgata cctgctccta ttactaaaat
     5341 tgcacctata atgtagaaaa ttgttgtacc aataccacct gttgaaggca actcagtacc
     5401 tttgttattt tcaacagttg ggttaactaa aaggttatct gaattagtcg tatcagtggc
     5461 tccatctcct aaaataacct tctgagagtt atctaacaaa ttgtaaccta agggagcctt
     5521 tttctcaact agatagtatg taccttcttt caagcctgta atggtaatta taccatctgc
     5581 tcctgttgta tattctgttg cattagcttc tgtgccccat tcaacgttat ttgtatcgtt
     5641 aaagtttaga aattgacccg tagcattctt taaaacaaat atagcacctt gtaatgaagc
     5701 ttttgtggaa ccatcaattt tttttatagt aatttgacca tccctcactg ttactttttg
     5761 acctgggtca tcattgctag tattggggtt gatggtcgca atgtttgtat tttctggtaa
     5821 atcagctgaa cctggtttag ctccactctt taatactcct gtataagtga ctgtgattgt
     5881 atttattccc ttataaaaaa agtcatcatt agctccattt tgagtatttc cggttggagt
     5941 attggtagct gcccacggaa tagtaatcgt gaaattatta ttttcctcta acaggttata
     6001 cttcccagtt gctttttccg aaccttgagt tagagttgta atattccctg atccatcagt
     6061 aatagttact tcataagacc cttcgttcaa atcaactaca gaagcagatg gcatagtatc
     6121 ctttataaca tattgataca ctttttctgt accatgataa ttgactgcat tcttataagt
     6181 aatagtatat ttgactgtat caccaaccga gtacgttttt tgatctacag tttttccacc
     6241 accatctccc catgtcgcat cagtattctt ttcatgaata gtagcatttg gagttacaga
     6301 tgtaaccata attacagctc cattattaac agtgctagaa acataataat atccatattg
     6361 ggaaacatta ataacctcag taccatcatt atttgactca gtaacagtgg aaactggtgt
     6421 agtattagct gatatagatt tagcccatgt cgcaatctca tttgctgacg cagtatcttt
     6481 tttagttaca tatgttctcc ctccattagt agttgtcgta aaaagagaat taaaatcagt
     6541 tgaagcttta tactcagctt ctttaccttg aggaattaaa taagaagctc catctttatt
     6601 cgaatcagat acatttgcat tatctatttc tgcatcaaaa actttatatg ctttataggt
     6661 tgcgcctttt tgagtatctt gaactgtaat tgtccctgtc tcagcggcaa aagctatcgg
     6721 cgtaactggt gatacagcca taccaaatgc taaactcgcc actaacagcg attgaatcat
     6781 tttctttttc attgaaatct ttctcctaaa atcatattga tgaatgatta attcatattt
     6841 tttttcgata gtataatatt aatcctgatg gtagagctaa agctaaacca actaggatat
     6901 aaatgtgtgt tccaatacct ccagtactag gcaattctgt tcctttactg ttagtaattt
     6961 taaaagtata tactgtactt ccatccacta aattctcttt tattggtgtc gcattattac
     7021 cattttgttc aaaggtaact cccgtagaaa tcactaatac tgacatatca tttttaggta
     7081 gtaggtaccc tggaggggcc tttgtctctg ttaggtagta ttttcctact ggcaaactga
     7141 ggtagttatt agcatccact aataacaagc ctttatcgtt tgtcaccagc cctgaataca
     7201 taggatgtga agctttattc ccattagcat ctgattcata aatatcaaaa actgcacctg
     7261 ctaaaaaatt attatcattt tcgacattaa ctttctgtag tcgtactttt tgcttgatac
     7321 gtgtattggt aaagctaata tctacgtctc ctgaaactgt cagggattgt aagccggtag
     7381 catcataagt tttatcagct tcaccagttg ctagattttt ttctgtaatt gactcagata
     7441 ctttaaattc atcgtaggct tgttcatcta ttgatataga agttccataa ggtactttaa
     7501 attccttagt ctgaccatct ctcagcggaa aattctcttg ttgcaacgtt tcacttggat
     7561 taaacaagaa gtctttcgtc ttatcttcat ctagtccaac gacagtttta cttactctga
     7621 cggtgtattc tttaggttgc caaacagcat ataaggtatt tgttgcatca gggttgttat
     7681 caatacctat tgattgacct gctgtaaatt ccacacgtcc tgtatcagct aaatccttat
     7741 catgatgcca accaataagg ttgtaacctg tccttgtaaa gtattggttt tcaggaattg
     7801 tagttgtgct attcaactcc atacgcggtg tctccacttg tgttaccaca ttaccatttt
     7861 ctactctagt accaccgtta ccattgtatt tgatggaggt atcttctaat ttgatatctc
     7921 ctactggaat aatgacaggt tttatggtga tatttttatt agcatctgct aaatgggcgt
     7981 caatatcaat ggaatcatat gggttataaa ttttaccatt gtaccaccag ccacggaaac
     8041 gatagccatc tggcattgtc ggacgtctca gtaaggctga atgggagcca tcgtcatagg
     8101 aatcagggtc agtaggctca ttacttgttt gtaattgctg accagaagca tccaatgctg
     8161 gctttccatc tgtaccaaca gcatcattgc tgtatataat atgataatct ccagcctttc
     8221 gccaaatagc tcttaaattg atatcttgag ttacagcacc tgaaaagtta taaggtctaa
     8281 tactaccatc tggattaaca taataccaac cgactaattt gtaagcgtct tttacgtact
     8341 tgtacttagt tgttgtatca acatttgaga gactagtatc tgtcgtataa taggcatctt
     8401 tagttgagtc gggatcttta tctcgtgaat catacttata ataatatgta cctgaaggat
     8461 cttggatata atcccttgta atatctgtat aatccggaat acgatcacca taatgcaaat
     8521 ctaaataggt atcatctgtt tttgataatc ggcctccgtt tggatcaata ttgacacgat
     8581 atgttacctt ttgccaacct gcatagactt taacatcatg aggaggcata gtcgtgttaa
     8641 agtcaaatac ttgtgtttgt gcttggtctt tataccattt accatcccaa acataccctg
     8701 gtcgactagg tttaggttga acagttgtcg tatcgggggc ataagaggac aaattttgct
     8761 catatagaac atcctttact ggaaaattag gaagctctgt attatcaagc ggatctaaat
     8821 atttaatctt gtatgaatta cgttcatacc ataccactaa gttcaaataa tctttgtggt
     8881 aggcaccata tctatcatag tattcatctg aaataggaac ttttactcct gcactcgttt
     8941 gacttgcgta ctgatcaaaa aggtaattat caggatataa acgttgataa tatttaacat
     9001 taaaccctag atatttttct gtaaaggtaa attcatctgc cccagcacct ccaccggtat
     9061 ctgctaaaga ataagtgcca tccaaacctt gtttgaagaa ccgataagtt tgaactatat
     9121 tcccttttgg aaagagtttg ataacatctg aatcaacggt atcattaggt aaaatgaact
     9181 cacccaaata actcatccca taggttccaa cttggttatt tccaacattg gctaaataac
     9241 cccatgcacc cgtcttccat tgataaccat tagcggccaa ggttgtaccg taaagtcctg
     9301 tgtaagtatc agcatcagag ctccaattat acgaatagta atattctggc aaggaatatc
     9361 cgccatattt tgcaaatttt attgtgatga gttttctatc ataataaaca ttaacaacac
     9421 ttgaaccatc gtcttttatc atgacagaag tttctgtcct cgtattatta actttaaagc
     9481 cagtcggtag tttatcatta atatcttgtt gtgttagcgt ctgattagat aaagataggc
     9541 ctgatcgtgt tacttgccct gcgtactcat atgtcttttg cgcatcagta gcatttttat
     9601 tatccgttgc tgattgttgc cagtagttta tcgtgtaggt tgtttgtgcg ggcgaccaat
     9661 gtgcatatag cgtcgtatcc ttggtcaaga cttgattaaa atcaaaggct gccccaccac
     9721 tagcagctgt gtaccaccct gcaaaagtat aacctggcct cgttggatca ttaggcttaa
     9781 ttgtcgaagc aggttggcct gttaacacac gacgaggtgc aatataggta actcctgttg
     9841 ataagtcgcc tgtgttgaat tcaacaccgt acgattcttt aaagacagga taaagactta
     9901 tgtctgaaga tacaggtaat gcttgaattt ctgattcaga aagtggagct ccattttgag
     9961 ttttagacca acctacaaat aacaatgttg aagtgggtgg tgaaacttta aatgaactta
    10021 aatccgcggt tccgtttcct gaactatcca atggtacttg ataacgctcc aaaatacttt
    10081 taccagcaga atcatcatat aaagttactg tcgccacttt actcataaat ggacgaacac
    10141 aaatttcttt tgtctcagtt acagttattg gctcaccaaa tttaacaggg tcaccatact
    10201 ttccagtagt aggatcatag gtataccaac cattaaaatg ctctcctgct ttaatcgtcg
    10261 gaatcccaac ttctcctaga gattctccat cttttataat ttgatgatga acttgcatac
    10321 ctgaagctgt caggaaatca taatcagttc cgtcattatt ttgaaaatgg taagttaacc
    10381 taggaacttc tgtattatcc tcttgaacaa ttgcataaat ggagaatgaa tctgttttaa
    10441 aagcaacatc actgctagtg ttcttagttt ctgcagtatc tttagatttt aatacttctg
    10501 tttgaccatc atctttaaag tgaacaactt taaggttttc atctgaagct tctaatggct
    10561 tatcatagtt aacctctact tttactggag cttggggttc tgcttcttta ccatttgact
    10621 caatagtaat gtcatagagt ttgaaatttt tgatttcact atcttgttta gcaacctctg
    10681 tcaatgcttt tttcttataa tctttaaaag tagctgaatt gtcttttaat tccgtcacct
    10741 ttaaatcagc atttttagga atcttagctt ctttggtcaa agtcactgtt acagtatagt
    10801 ctgcacctct aaacatcaat ggttcttcac gataagcagc ttcctcagaa gatgatgttt
    10861 ctgttacact agaagcagga gtctggggct tgctctgctc aacacttgat tgagaactag
    10921 atgttgatga agttacctgg ctagaatttt tattttctaa agtaatcccc acatcatctg
    10981 tcttagtttc ttcaactgtt attgctggta gaattaaaaa ataagtcgtt aaaaaagttg
    11041 ttaggatcat caatgaccac atgataattt tccactcttt agggtgtttt ttctttttta
    11101 atgattcgat tataaaagtt tgacacttct ttagcatttt tgcatcctcc ctaaccttaa
    11161 ttgatactac taatcttacc tagaggccat attctgaaag aaattttacc tacaatttgt
    11221 tcttctgaaa catctcctac agaagtactt cgagaatcaa ttgaagtttt tcggttgtct
    11281 cctaatacaa aaattttttt atcaggtact tgatatgggt attttatatt actattaccg
    11341 agtgctttat gaataacata tggttctttc aacttatgtt gattcacgta aacatcccct
    11401 tgagaatcaa tattaaccca gtctcctgac tctgcaataa cccgcttgac taggacctta
    11461 ttattgtagt aaaacgcgac aacgtctcca gttttaaaat ttgaaccttt tactgtaaag
    11521 actacatcac ctgcacttaa agtcttattc attgaatgtc cgtagattct taatacaggc
    11581 aaccataaaa ccgcaattaa aatggctatt gaggcaaccg ccatcaagat gtatatggta
    11641 tttttaatga cactccaaaa gtgtttctga taagttacgc gatccagttc ttgagataat
    11701 ttatctgaac taatctgtct tttcattgtc tacgcctctc ttatctacta aattctttac
    11761 gttttctaaa taggtatctg cagctaactg agcagtctca aaaataccac taagcattaa
    11821 ggaagcttcg gcaatagaac cagctttgct aattttgatt tttttatcat ctagcgcttc
    11881 tttaagttgc tgaatctctt tctcttgttt ttcaataaga agttgctgtt ctaacataat
    11941 ttctagcaag tcttttcttt ttaatttttt taaatcttcc atcgcgatta cttccttaac
    12001 tgaaccttaa attatcgttt agatattata tcaaagttct aacctttaaa ctcatttttt
    12061 gtcctgtgtt ttttctcaaa aaagtctatg ctaaattaac atttttgata attttttgaa
    12121 aaatctcatc gaagtcattt tctttttgaa agctcgaatt ctaggcatta aaaagccata
    12181 tatcaaattg atatatggct ttttttatta tttaaaacaa aagaatcaat aggacaatag
    12241 cgtcaattta gtgacataat ctattacaga ttaagttctt tttgaataat ataatccaac
    12301 ttttcaactg ttttttccca tgtgaaatgt tctttaattc ttttagcaat attctgttgt
    12361 agtttctctc ttaatgcctt atcttttact aataaatcaa gagattcatg gagtgactga
    12421 gtattttctt ccatgatgat tcctaactca gggctatcaa taacttcaac tgttccaccg
    12481 cgatctgttg caataatagc actcgaaagt agaccagctt ctaaaataga ggttggtaat
    12541 ccctctggat acattgatgg gtaaacaaag atatctgtct gtgccattaa agacatagtc
    12601 tgttcaaagt ttaatttccc caaaaagtta atctgtttgg actgatattt ctctttcaaa
    12661 tgtgctaatt caggtccgtc tcctgcaatc tgtaaataaa cattttcaga gtactgtgac
    12721 atcgaaaatg cttctaagag caattcaatg cctttttctt taataattct accagcataa
    12781 gtgatgaaaa tatcatcagc agatttttca aggtaagccg tgtcagcaaa atcagaacct
    12841 agactttcag ataccgaatt ataaataact cctttagctt ctatattaaa atgttttaac
    12901 cactcaacgc ttctcttaga taccgcataa aaatctggac gatagtgctt aacacgcgct
    12961 gtgagaagat gttcatagat agctccaaag aaatctaaaa aacgattatt gacagaaaaa
    13021 tgacttgacc catggtctaa aacaatacta ggtaaacggt gcttctttgc aaaagatagc
    13081 ccttctagcg ttgttaactg aaaacgtgta ttacaaatca caaaatcaat attttcatct
    13141 gaaacatgtt tcatcagcgt gttgtattct cgatttttgt taataatagg atagcgctgc
    13201 ttgacaatat ttttggtcgg taaacggtaa atttttctac ccttgtcttc atctataatc
    13261 ggtaaatcat catgattagt tgttacaata acaacacggt agccacgctt aaccaaatct
    13321 gctgtcattt tatctgtata acgttcaata cctccgagga agggtagata atatcctgag
    13381 aaaacagcaa ctgtttttac cttattttcc atatttatcc actttcatca ataagccatc
    13441 ttttaagcct ttaatcatag caactaattt tttgctcttt tgctcttctg ctaccaacac
    13501 tcgaacaaat tcatttcgca taaatactaa atatttgtgc cgcttcttct taccatattt
    13561 ttttataata taaatcgcat tgcgtatcat gtaatatttt cgaaatggtg aatgattcaa
    13621 tacatgaaaa acatggccaa attttttaac tcgtgaagag tgtccaattt cgtgtaaaag
    13681 accaataaaa ttaacctgat aagtcttata tcccatctct gacagacgat aattcatttc
    13741 agagtcaaca aaatcaataa acatctcttc tgcaaagcca gatgtttctt caaaaacgct
    13801 cgttttcatt aaagcagccg aagtaataca ctcttcaatt tctttatagt caaattcttg
    13861 cataactaaa tcttcacggt tcatatcttg atacaaacaa gataacatac cgaccttagg
    13921 taaaagaagg taattttcat aattatctat caaatcacct aggacaaccg aatcttgatc
    13981 taaagtcaag aaccaatcaa attcttgtgc tactgcaaat tgaccgatac agttcaaagc
    14041 atatgcaatt cctttatttt ctgttaaata atcaacagtt aggtgcccct cttcattata
    14101 atcggccact aattgagaaa tttcttcctt atttttcgag ccattatcta cgatgtagat
    14161 atggcttact tgaggataaa ttgctcgaat gttctgatct aagcgttcaa tattggggtt
    14221 aaaggtgaca atacccgcta aatatttcat gttctatgct cttttctaaa atctctaaat
    14281 aactgaatga ctggtgcttt ggttataaaa acgataccga catagatagt tactgctact
    14341 aaactttgaa tgacataatt taccaatgat actgacattt gagtattgat ataataaagt
    14401 acagctccac taagagtagc agcaattaaa tagcgcagca ttcctcttgt taattcttta
    14461 aaagtaaata catctcttaa agagatagct tgatagaggg agacaataaa ttcagtaata
    14521 actgtagaga taatagctcc catagcacct aaaattggta ttaaaagtat attaagcaca
    14581 acatttgcca caagtccaat aactgcagac attgtgtaag cttttgtacg tcttgaagcc
    14641 agtagatact gtgtccctaa agcgttacca taagaaatgc aaatgatcat caaagactca
    14701 acagccatta gcggaccgac agcttcatac cctttcccaa aaaagaagac ggcaaatgtc
    14761 gacgaaactc ctaccactcc cgcaatcatt aacattgaga tagcattgct taaatcaaca
    14821 agtttcaaaa gtaaagcctt agcttgcttt tcttttcctg aagaaaatag actggagagc
    14881 cttggtaaga aaactcctcc gattgctgaa acaatggtaa ataaaatacg aataatttta
    14941 tcagattgat caaaatatcc agcactgacc acactatcca ttgctcccag catactcttg
    15001 ttaagagaga gataaatctg catggtgatt tggggtaaaa ataacattaa agttggtctt
    15061 agatgtatta agatatctaa tctactaaat ggtatcttga tgatttcatg ctttaaatga
    15121 tgccagactg ttaaatttcc gagtaatgat gcaaatgcaa ttaaaaacat ataaagtgct
    15181 aggtcatctg gcgtcttcac aactaagaag gtcaaaacaa ctgtaataag cttaacaatg
    15241 gtattacgaa tgacaattat cttaaagtct tcaactccaa taaaatacca cgaaatatca
    15301 gttgcagtcg ctaataaatt aataccttga attaaataga ataattgcca ttcgtttgta
    15361 agtaagacaa atccaaagaa taataacgtt gctatagata cagaggcaag ttttaataca
    15421 actaattccc aaaatatctt acttctctta tacttatttg actgcacaaa agctatctca
    15481 cgattaccgt acaaagatat ccctaaagca ccaaataaag taaagtacat gacaatggag
    15541 ctcgtatagg cattaatacc gatgccttga ggagatagta cacgggaaac ataaggaact
    15601 gttactaaag gtaacaacaa agtcaataac tggtatgagg tattataaaa catatttttc
    15661 aaaagtttca ttagtttctc ctcataatta aactaataac agtatcaaat aacaataagg
    15721 catttgatac aattaccaag ccagttaacg catacggcac cactttcctt cctgcctcat
    15781 ttagacgcgc tggaatactt gatgcaaaaa cttgtaccaa aagcaatgta acagtaaagt
    15841 aacgtccttg aactccttca atgtagcttg ttggcgctcc acgatattct aacatgataa
    15901 aaaagacacc aagaattccc acaaagaaac tagctaagaa aagaaatccc atattttttg
    15961 acttaacaaa tttttcttca gaaaaccata ctagaaagac catgagatac catactgatg
    16021 atacccaagt gggaataaaa ttctgacttg gatatggaga ggcaagccag gaagtaatgt
    16081 cattacttaa aatattgata ccagcataat tatagccaaa tgtcattaca taacgtctta
    16141 aaacagcaag gacaccacca tgtcttgcgg tcagaacata aactatagca agtgacaaaa
    16201 aggttagaca tagccaaaat agctttttag atttaagacc ttgaaataaa tgtgatagct
    16261 tctgattata gcccattaat ttaggattgt taacctgcaa taataccggt attactagca
    16321 ataaaaacag ccagtaattc tgcttacatg caacaatgac taaaagaatc aatcctataa
    16381 gagcaatact acgattttta gtcaacgatt tatcaactaa aagatttatc aaaagcgata
    16441 aaaatgctcc aaatacaata tatccagtcg agtcataaga tagactagca aatgaattta
    16501 gaactacggg actgaggaaa atagcaaagt agattaactt tccttttttc acataccgga
    16561 taatgagggc aaccaaggtg attgagacaa aggaactaaa taaccttgct actgtaatca
    16621 ttacccccat agaaggatac acaaatttgc ctaaaaataa gccaactgct ggcacaagat
    16681 gaccccaaaa aacaaagtta tgtttagaat agtttatctc acgaggcaaa tgattaccat
    16741 caacaaaatt ggctttattt aaatagtatt tttcaaatct agtaccatct ctataggagg
    16801 tcgattgtcg tctcatacct gaatcaatat tatattctcc atagtaagaa gtatcaataa
    16861 tgttatttga aatacgacca gacacagcta aatggtattg tccatcaggt tcattgaaaa
    16921 atggcattaa taggctcaat acaatagtaa tcgttaagcc taatataaaa tatgttttat
    16981 gcaagttctt gtctagaaaa aacttttcaa atagacgaac ttttttgagg ttcatattaa
    17041 ctccttttta tatcttttaa taaaaacacc aagtcacgaa tgcgatttga aaagctatca
    17101 tagaaccgtt ttttagtcat catttgttta agtattgcta caaaatcttt attttgagca
    17161 tttttataac gttgctgatt aaaatccaga taagctttag cttcccttaa ttgactagag
    17221 ctactttttt ctaaaaatgg tacaaggatg atatagtaac tcaaaacaaa atacagttct
    17281 tgtaaatgcc tttcgtaact tgagctcata ttcaagatat tcccacttgc attctcagca
    17341 tgtcgcttat gttcaccaac acttctgttt aaattgtaac ctgaaccaat gacaccagat
    17401 acccctgcaa aaagattatc atgtgacaaa attaaattat cactgtaaaa agtcattact
    17461 tgatcaacaa acgacttgcg taaacaaaaa gtccaacctt gacgatagtt atgatatgaa
    17521 aagtctaatg gatacttaga aattgtttcc tcaccattga acttaaaatt attgggaata
    17581 gtagcctctt ctgataactt tttgatatca atatcggcgc taagaaggtt tatatcttgg
    17641 cgttcttcca taatatcaac ttgcatctta tttttatgat gataccatgt gtcatcttga
    17701 tcactaaaaa agacatagtc aacctcatag gctaacacat caataagtaa ttgacgaaaa
    17761 tttaaacgcc aacctaaatt tttatcatta ctagttattg accaaccatc taaacggtgc
    17821 tcttttatat agtcttcaac cacctttaca gtatcatcag tagagccatc gtccctcatt
    17881 aagacatagt ctggtctcaa cgtttgattt ctaattgaat ctagctgatc gtggatgaaa
    17941 ccttgaccat tatatgttgc catcaaaact gctgttttta ccatattatt ccttactttt
    18001 taaacgcaaa taataaatct ctcatacgtc cactattaga gttatagagt cgccaatttt
    18061 taatcacaaa agcactatta tgaattaatg acaatttaga agcttccaat ctttcttttt
    18121 caaaggctaa gcgttgattt aaagcgacat cattttgata tttgttgaca accttctgca
    18181 tggtttcgta aaaatcaaca aatgttttta atcctgtgat atgttgtgtt ttaggcgact
    18241 tccaattcaa aacaaatttg ctattaccac ttgcgtttcc tccatatcga atatgaactc
    18301 ctacgctttc gttaaggtta taaccatttc caataatact ggcagttgtc tcgataagat
    18361 tatcgtgcga aatattagct ccaaaagtcc aattatcagc gatagcttgc gcaaaatctc
    18421 tcttcatagc aatggtccat ccgggacgga atgtttgata atcatatact ttaggatact
    18481 gactgattct atcactagaa gaaaaagtta gaaaatgtgg aacactggct tctgtagaca
    18541 tcgttttgat atcaacgtct gcactcaaaa cctcaatttg agggttatct gacataatgg
    18601 caaactgtcg ttcgttttta tcaagatacc aaatatcatc ttgatcacta aaaaagacat
    18661 agtcaacctc ataggctaac acatcaataa gtaattgacg aaaatttaaa cgccagccta
    18721 agtttttgtc gtttttaaca attttccagc cttctaactc atgttttgcg atatagttat
    18781 tgacgacatt gactgtttca tccgttgaac aatcatccct caataataca taatctggtt
    18841 ttaatgtctg ttggcgaatt gaatcaagtt gttcagatat aaatttttcg ccattgtatg
    18901 tcgccattaa aactgctgtc tttaccatat tactcctcaa atgcttgtac tgtttttaat
    18961 attccagatt caagagaaac ttctcgcttc catcccaacg cttctaattt ggccatagtg
    19021 agacgtgttc tccccatttt gctataacct gcactcatag ttttaggaat atcgaatact
    19081 actgaaatat ttttatcaga aaagagatca actaactttt gtgccaaatc cttaatcata
    19141 atgggttgat cttcatttgc tacattgtaa gcttgaccaa cttcaccatt taataaaata
    19201 gtaaataacc ctgatacagc atctgctaaa taacagaaag cacgctctgc tgtaccacta
    19261 ctttttaaaa cgatatcttt gccatcaatg acattcgata aaagatcatt cataatacga
    19321 ccatcgttac caagctccat accaggacca aatgaatgtg caatacgagc aatagtaaag
    19381 ggtaccttat attgatcata gtaactttgc agtagagttt cagccatacg tttgctttct
    19441 gggtaacaag ctctagtagc taaaatatca aatccaccat aagcttcttc atcaatcact
    19501 tctttaatag atgttccata tacttcacgc gttgataaga acaagaagtt ttcaattgtt
    19561 ttttcctttg caaagtctaa taaattcaat gtaccaattg tattagcttt tatgatgcta
    19621 acaggattac tcaaaatagc agttggacta gcattgcttg ctgcatggat aatgtaatcc
    19681 acttttccat catactctag gcgttcttct acatcataag gaattaaggt gaaataatct
    19741 ttcccaacta aatctgaaaa tttatcacgg gctttttcaa tgtttctagc tgtcgcaata
    19801 atttgcgttt ggtaatttct tgtctcattg agataggcta gtaaaaatac catataactc
    19861 gctaacatac tattagaacc acttattaaa acgcctttat tagcaattct ttcccaatta
    19921 atataatttt ggttactaat aagtgctaaa tcttcttgta caattttatt actaaattta
    19981 atcatcatta catcccataa atttgttcat tttctctagc atcatacaaa gctttaaaca
    20041 tataaaaatc atctggtgtt gttatcttga tattttcata tggtccctca acaatagtga
    20101 gttcacgatt gtacataccc atcaaagtac tagaatcaat tgcatcagta atccctttgc
    20161 taatggcatc acgttcaaca gaaaggatat ctgatagata aaaactttgt ggcgctttag
    20221 caatgaagga acgagtacga tcaacaactt cagatatttt agaagaatca ttgactagaa
    20281 caactgtctc tttagcacga actgaagtta ccgcacttcc tgtttctttt actgactgta
    20341 tattagcatt aataacttct tcattaatca atggacgtac tccgtcatga attaaaacaa
    20401 cggcatcatc tgtagccagc tgttcagcgg cttcaagccc cttaaaaatt gacatttggc
    20461 cagtttctcc acctgcaaca actgctttta ctttcgtaag gttgaaacgc tctaccagat
    20521 tattcatata atctagccag tctgatacac agacaactac tacagcatca atagcttctg
    20581 tattttggaa aatatcaatt gtgtgtacaa taatgggttt accatgcact tctaggaatt
    20641 gctttggttt acctttggta ttcatgcgac gaccaacacc acctgcaaaa ataactccaa
    20701 tattcatcat taaccttctt tccagtacca agtgtaataa ctatgacttt ctctcttact
    20761 ttctggaggt aattccatat aactaccccc atataattga ttcagataag catgctcatt
    20821 tttaattttt cgggttttaa tgttttcaaa cataacatct tcatactcag aaaaaatttc
    20881 tcttggaaaa tattctttca agcgatatct tgaatcggta taacccatgt aaggaacctc
    20941 ttgattatta tatgtttcca tcacttgatc aacaatttca gcacgttttt tagcttggcc
    21001 gtgataagcg atgaatcggg gtagccccaa aattagcctt gcatttgcat aaagctttcg
    21061 ggaaccgcta gcaaaattgg ggtaagtcgt acacatcaaa tttgcagcag cagagtgaac
    21121 ttttttaaag aatctttgac gttcttctgc actctctggt aatcggtcaa gtgggaagat
    21181 atcaataaaa atccctgtcc aaggatgaat tctatctgtt ttacttaagt acattgactt
    21241 tgtgtgatac agcttggcaa atggccatag atttttttca acagaatgat gtagcaattt
    21301 aaaatcgcta ttattagcat cagctaatgc tttcatcaaa cgctcatact ggcttctttc
    21361 tagcatcaaa tcaatatcgt catcccatgg aatgaatcct ttatgtctca ttgctcccag
    21421 taggctgcct ccgcctaaag aatattcaat tttattatca cgcgcaactt tatcaatata
    21481 agctaacatt tcaagttgaa cttctcttat ttctgagacc gtcatttctt tcataattac
    21541 ctctgtctaa cagttaactc acattctttt aaatacaaat cttatagctg taatatagaa
    21601 aataaataaa gctactgcaa aaagaatcac tagggtcatg ggtttgaaag ttttctttgt
    21661 tttttgacct tttggagtgt ttgcaagtgc attgtattct tgtttgttgt actgcatgtc
    21721 cattata
//
LOCUS       DATROQ010000029        21494 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.029, whole genome shotgun sequence.
ACCESSION   DATROQ010000029 DATROQ010000000
VERSION     DATROQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21494
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.029"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            30..647
                     /gene="eda"
                     /locus_tag="VBC94_000962"
     CDS             30..647
                     /gene="eda"
                     /locus_tag="VBC94_000962"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO6979241.1"
                     /translation="MLNQLKNNYFFAVIRGKSSEDALEIAKHAILGGIRNIEVTFSTP
                     EASKVIKQLSDDFKNNKEIIVGAGTVMTTELAKEAIDAGAKFLVSPHFDSDIANLANE
                     NKVYYFPGCATATEIVVARKYKCQIIKLFPGGVVGSGFIKDIHGPIPDVDLMPSGGVS
                     VSNVVEWRKAGAVAVGVGSALSSKVATEGYDSVTKIAKQFVSALD"
     gene            664..2064
                     /gene="uxaC"
                     /locus_tag="VBC94_000963"
     CDS             664..2064
                     /gene="uxaC"
                     /locus_tag="VBC94_000963"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEO6979242.1"
                     /translation="MAFNTETFMLKNQAAIQLYEEVKRQPIFDYHCHLDPKDIFEDHI
                     FDNIVDLWLGGDHYKWRLMRANGISEAEITGPASNLEKFKAFARTLERAYGNPVYHWS
                     AMELKNIFGVNEILTESNAEEIYHRLNHFLKEHKISPRRLIADSKVMFIGTTDHPLDT
                     LEWHKKLAADESFKTVVAPTFRPDEAFIEHRHFVDFITKLGDITQKEITDFSTFIAAM
                     EERIAYFAQNGCRASDISFTEIVFEQTDELELNDLFNKVCEGYIPNQSEISKWQTAVF
                     MELCRLYKKYGFVTQVHFGALRNNHSTIFEKLGADVGVDSLGDQVALTVNMNRLLDSL
                     VKKDSLPKMIWYNLNPAYNIAVANTLANFQANELGVRSYLQFGAGWWFADTKLGMISQ
                     MNALAEQGMLANFIGMLTDSRSFLSYQRHDYFRRILCTYLGEWIEEGEVPEDYQALGS
                     MAKDIAYQNAVNYFKN"
     gene            2082..3128
                     /gene="uxuA"
                     /locus_tag="VBC94_000964"
     CDS             2082..3128
                     /gene="uxuA"
                     /locus_tag="VBC94_000964"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEO6979243.1"
                     /translation="MEMSFRWYGEDDPVTLENIGQIPTMKGIVTAIYDVPVGEVWSRE
                     RIQQLKEKVEAAGLKISVIESVPVHEDIKLGRPTRDLLIDNYIQTVKNLAAEGIDTIC
                     YNFMPVFDWTRTDLAYQYPDGSTALIFDETVSKKMDPVNGELSLPGWDASYSKEEMKA
                     IMDAYAEIDEEKLWENLTYFIKRIIPEAEAVGVKMAIHPDDPPYSIFGLPRIITGLEA
                     IERFVKLYDSKSNGITLCVGSYASDPQNDVLEISRRAFELDRVNFVHARNIKLGDGKS
                     FKESAHPSEYGSIDMYEVIKLCHEFGFEGAIRPDHGRMIWGETGRPGYGLYDRALGAT
                     YVSGLYEAVIKGSK"
     gene            3252..4091
                     /locus_tag="VBC94_000965"
     CDS             3252..4091
                     /locus_tag="VBC94_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO6979244.1"
                     /translation="MSKTITFTNKVVVITGAGGVLCGYMAKEFAKAGAKVALLDLNQE
                     AAQTFADEIVEEGGIAKAYKANVLSKENLEEVHQAVLEDLGPTDILVNGAGGNNPKAT
                     TDNEFHELDLPSETKTFFELDEAGISFVFNLNYLGTLLPTQVFAQDMVGREGANIINI
                     SSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVGIRCNAIAPGFLVTNQNRSLLF
                     TEDGQPTARAEKILNNTPMGRFGEASELIGGLFFLADEKSSSFVNGVVLPIDGGFAAY
                     SGV"
     gene            4244..5056
                     /locus_tag="VBC94_000966"
     CDS             4244..5056
                     /locus_tag="VBC94_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6979245.1"
                     /translation="MVDTNHKIAFCIDSDGCAMDTMTYKHQLFFGPIAADIFGISEQE
                     RTSFLKEWDRVNLYSRTRGVNRFVGLVMGLEFAGVEHIDKLKEWVETTDSLSNNSLEL
                     ALANDFSRDLEKALEWSNEVNRQIKNYEGDSLAFSGVLESLERLSQFGNVYVVSSANK
                     EAVWQEWEEQGLLKYVDDIYCQDRGKKEDVIATLIASGYCRETILMVGDSLGDLAAAE
                     QNGIAFYPVLVGKEVKSWEILREDIGEAFAKGQFEQQRQKESINTFWANLDN"
     gene            5072..6862
                     /locus_tag="VBC94_000967"
     CDS             5072..6862
                     /locus_tag="VBC94_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEO6979246.1"
                     /translation="MTHLVDLTKKPFNLNQEAIEWIEKTINEMTLDEKIGQLFFNMGA
                     SRSEEYLTDVLDRYHIAAVRYNRGSSSEIYDQNLILQTKSKLPMLIAANTEAGGDGAV
                     TDGTKVGDEIKVAATNDPKYAYEMGRIAGMEASAVGCNASFSPIVDLTRNWRNPIIAS
                     RNWGANVDQIISLSKEYMKGIMQYNIVPFAKHFPGDGIDERDHHLSFASNPMSKEEWM
                     STFGRIYGELADAGLPGVMAGHIHLPNVEKEMHPERDLDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMVGMTASMPRRDLLPTAIEAGCDLFLFFNDPDEDIQWMKEGYEKG
                     ILTEERLHDALRRTLGLKAKLGLHNYEGRRQELFMPKDKAMALINTLESQKIADEVAD
                     KAVTLVKDKQKDIFPVNPERYRHILLVNVEGYKGGFGAMIAGNKQRASDYMKELLEAR
                     GHEVTVWESTEERIMKLPQEERAAAIANVYAQKQPIANLTEHYDLIINLVDVNAGGTT
                     QRIIWPAAKGTPDQPFYVHEIPSIVISVQHAFALADMPQVGTYINAYDGLPSTISAVV
                     AKLAGESEFTGVSPVDAYCGLIDTHLWRQC"
     gene            complement(6920..8005)
                     /locus_tag="VBC94_000968"
     CDS             complement(6920..8005)
                     /locus_tag="VBC94_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO6979247.1"
                     /translation="MSKLNRIRHHLHSVQAELAVFSDPVTVNYLTGFFCDPHERQMFL
                     FVYEDRDPILFVPALEVSRAKQSVPFPVFGYIDSENPWQKIASNLPSFSVSKVLAEFD
                     NLNVTKFQGLQTVFDGHFENLTPYIQNMRLIKSRDEIEKMLVAGEFADKAVQVGFDNI
                     SLNNTETDIIAQIEFEMKKQGINKMSFDTMVLTGNNAANPHGIPGTNKIENNALLLFD
                     LGVETLGYTSDMTRTVAVGKPDQFKKDIYHLCLEAHQAAIDFIKPGVLASEVDAAARN
                     VIEKAGYGQYFNHRLGHGLGMDVHEFPSIMAGNDMEIQEGMCFSVEPGIYIPDKVGVR
                     IEDCGYVTKTGFEVFTKTPKELLYFEG"
     gene            8215..9219
                     /gene="ccpA"
                     /locus_tag="VBC94_000969"
     CDS             8215..9219
                     /gene="ccpA"
                     /locus_tag="VBC94_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEO6979248.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILARGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPIVLAGTVDLEHQLPS
                     VNIDYKAAAVDVIDILAGNHKDIAFVSGPLIDDINGKVRLAGYKEGLKKNGLNFKEGL
                     VFEANYRYAEGFALAQRVINAGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPIAQYTRPNLTSISQPVYDLGAVSMRMLTKIMHKEELEEKEIVLNHGIVKRGTTNN
                     "
     gene            9351..10817
                     /locus_tag="VBC94_000970"
     CDS             9351..10817
                     /locus_tag="VBC94_000970"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEO6979249.1"
                     /translation="MTNELIMQAFEWYLPSDGNHWKKLEESISDLKKLGISKIWLPPA
                     FKGTSSDDVGYGVYDLFDLGEFDQNGTIRTKYGRKEEYLKLIKSLKANGIKPFADIVL
                     NHKANGDHKEKFQVIKVNPENRQEALSEPYEIEGWTGFDFPGRQGEYNDFKWHWYHFT
                     GLDYDAKNNETDIFMIVGDNKGWADDDLIDDENGNFDYLMYNDIDFKHPEVIKNLQDW
                     AKWFIETTGIEGFRLDAVKHIDSYFIQTFINDIRTKIKPDLEVFGEYWKSDQTSMKDY
                     LEATQFQFSLVDVTLHMNFFDASHQNRDFDMRTIFDDSLVIDNPEYAVTFVENHDTQS
                     GQALESRVEDWFKPLAYGLILLRQQGTPCLFYGDYYGIQGEFGQPSFKEVIDKMAELR
                     QNYVFGEQVDYFTHSNCIGWTCLGDEEHNSCLAVVLTNGDQGWKHMEVGEIYAGKTFV
                     DYLGNCEQEVVIGDDGWGDFLVASASISAWVPKIEEAN"
     gene            10862..11860
                     /locus_tag="VBC94_000971"
     CDS             10862..11860
                     /locus_tag="VBC94_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6979250.1"
                     /translation="MKVLLYLEAEEYLKKSGIGRAIKHQEKALQIAGIDYTTNPTDDF
                     DLVHMNTYGIRSWLLMSKAKKTGKKVIMHGHSTEEDFRNSFIGSNLVSPLFKWYLCRF
                     YQKADAIITPTDYSKQLIKAYGIKKPIFVLSNGIDLSRYQRSEKKEAAFRHYFHLSKD
                     DKVVMGAGLYFMRKGIDQFVEVAAKMPDIRFIWFGETNKWVIPRKVRQIVTKQHPSNV
                     TFAGYIKGDVYEGAMSASDAFFFPSREETEGIVVLEALASHQHVVLRDIPVYHGWVTE
                     DSVELATDVDGFVEKLDKVLSGKSDKIKEGYRVAESRSIERIAHELASVYQKVMEL"
     gene            11862..13196
                     /locus_tag="VBC94_000972"
     CDS             11862..13196
                     /locus_tag="VBC94_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO6979251.1"
                     /translation="MRIGLFTDTYFPQVSGVSTSIRTLKEGLEKEGHEVYIFTTTDRN
                     VKRFEDPTIIRLPSVPFISFTDRRVVYRGLISAYRIAKDYELDIIHTQTEFSLGLLGK
                     LVAKALRIPVVHTYHTQYEDYVGYIAKGKLIKPSMVKYIMRTYLSDLDGVICPSRIVL
                     NLLDGYGVKIPKQVIPTGIPVENYRREDISEETIKNLRTELGLADNDTMLLSLSRVSF
                     EKNIQAILMHLSAVVDENPHVKLVIVGDGPYLSDLKELVHSLELENSVIFTGMVEHSQ
                     VAIYYKACDFFISASTSETQGLTYIESLASGRPIIAQSNPYLDDVISDKMFGTLYKKE
                     SDLADAILDAIAETPKMTQEAYEQKLYEISAENFSKSVYAFYLDFLISQKASVKEKVS
                     LTIGNKDSHSTLRFVRKAVYLPKKVFTFTGRASKKVVKAPKRRISSIRDFLD"
     gene            13653..15596
                     /gene="thrS"
                     /locus_tag="VBC94_000973"
     CDS             13653..15596
                     /gene="thrS"
                     /locus_tag="VBC94_000973"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEO6979252.1"
                     /translation="MIKITFPDGAIREFESGITTFEIAQSISNSLAKKALAGKFNGQL
                     IDTTRAIEEDGSIEIVTPDHEDALGVLRHSAAHLFAQAAKRLFPDLCLGVGPAIHDGF
                     YYDTDNKSGQISNDDLPRIEEEMKKIVKENHPCIREEISKEEALELFKDDPYKVELIS
                     EHAEDGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNAMMQRVYGT
                     AWFDKKDLKAYLKRREEAKERDHRKLGKELDLFMVNPEVGQGLPFWLPNGATIRRELE
                     RYIVDKEIASGYQHVYTPPMASVEFYKTSGHWDHYREDMFPTMDMGDGEEFVLRPMNC
                     PHHIEVYKHHVHSYRELPIRIAELGMMHRYEKSGALTGLQRVREMTLNDAHIFVTPEQ
                     IKDEFLKALNLIAEIYEDFNLTDYRFRLSYRDPEDKHKYYDNDEMWENAQAMLKEAMD
                     DFGLDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPIMIHRGGISTMERFTAILIETYKGAFPTWLAPQQVSVIPISNEAHIDYAWEVARA
                     LKDRGIRAEVDDRNEKMQYKIRAAQTQKIPYQLIVGDKEMEEKAVNVRRYGSKATETK
                     SIEEFVESILADIARKSRPDEVK"
     gene            15817..16521
                     /locus_tag="VBC94_000974"
     CDS             15817..16521
                     /locus_tag="VBC94_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6979253.1"
                     /translation="MRTVLVVQGDDETIELLRSYLEGALYKVVMASDGEEAFSLFQQH
                     QIDLAIIDITLPKIDGYELTRLIRQDSQIPIIMLAAKTTDMDRILGLNIGADDFITKP
                     FNSLEVLARINSQLRRYYEFNSLAKPKNQFIKIGELELDEEHVELTKNGKHIKLTATE
                     FKILHILMSSPGKIYTKTQLYEKINGRYLEGDETTIMVHISNIRDKIEDDSKYPKYIK
                     TLRGVGYKIEKPQYLV"
     gene            16619..17638
                     /locus_tag="VBC94_000975"
     CDS             16619..17638
                     /locus_tag="VBC94_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEO6979254.1"
                     /translation="MAKTALNIPFTVEGIENYYQNIKMILSLADKWSIPKDDLLLFLK
                     GLKAIDDIKVGSPIFKYFWTSLSLQAPLKALEFVLEQAKMPTELSGELSETQYLVAQF
                     SDELAPHDDFWIALSQVIYDSFPGNSLAEDTVLNRKLHQFRYLISSQQAQYVRRYFKD
                     VGMTDRDALVNYLSCLREPDSIAYYESARLHNKRRRNGEIFGFPDDEPVINSKLLISF
                     HTEFIIDDKGNFLNEIDAEVITRNGIINGASFNYAFKNNTRHKELDVDPVKLDPKFRN
                     DMTRGYRSPNLSRRKWFFFKEEDYDCSYFNKKGYYAFGRRSAKQSVDKQVKYLKKAVQ
                     KMRIN"
     gene            17805..18371
                     /locus_tag="VBC94_000976"
     CDS             17805..18371
                     /locus_tag="VBC94_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO6979255.1"
                     /translation="MIKKNKVFLGVLLVLVVILGGGVLFYQSQFQKTTNQALAIAYKD
                     AKVAKKDVIHQKIDKEFENFRGSYEIEFNTKSAEYSYHVDAKTGQILERDMDNNGFSK
                     STSQSSSSSSQKSHKISQEEAKKIAFKDANIEESEVSNLKIKEEIENGKSVYDIDFVD
                     LKNKNEVDYQIDAETGKIIERSRDHMND"
     gene            complement(18420..19070)
                     /locus_tag="VBC94_000977"
     CDS             complement(18420..19070)
                     /locus_tag="VBC94_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6979256.1"
                     /translation="MSSVLTLTNILFLLQGFGLTLYISFISILLSMFFGTLLAIMRNS
                     KNPIWKLIASIYIEFVRNVPNLLWIFIIFLVFQMKSVSAGITSFTIFTSAALAEIIRG
                     GLNGVDKGQTEAGLSQGFTYLQVFIIIIFPQAFRKMLPAIISQFVTVIKDTSLLYSVI
                     AIQEIFGKSQILMGRYFEAGQVFTLYAIITAVYFITNFIISSFSRKLSKRWEQATE"
     gene            complement(19082..19777)
                     /locus_tag="VBC94_000978"
     CDS             complement(19082..19777)
                     /locus_tag="VBC94_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6979257.1"
                     /translation="MTLLNISPFAISRWGAFFNHFDLFFKGFLYTLGISFGALLLALI
                     LGILSGGLSTSKSKVGKLISRIYVEVFQNTPLLVQMVFVYYGLAIISNGHIMISAFFT
                     AVLCVGLYHGAYISEVIRSGIEAVPKGQTEAALAQGFTANQTMQLIILPQAIRTILPP
                     MTNQVVNLIKNTSTVAIISGADIMFLAKAWAYDTTNYIPAFAGAAIFYFVICFPLASW
                     ARKQEELNKKTYS"
     gene            complement(19790..20590)
                     /locus_tag="VBC94_000979"
     CDS             complement(19790..20590)
                     /locus_tag="VBC94_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6979258.1"
                     /translation="MLRRKRLTLYLLSCIFIFLLFYPNSTSANQLSEIKKSGVLKVGV
                     KQDVPNFGYYNAETNQYEGMEIDIAKKIAKSLGVKPVFVPTTAQTREPLMDNGQIDIL
                     IATYTITPERKANYNISKAYYHDEIGFLVRKNSHIKTIKELDGKHIGVAQGATTKVNL
                     EKYAKEHKLKFSYAQLGSFPELAISLYANRIDAFSVDKSILSGYLSPHTTILKEGFNT
                     QEYGIATSKQDKVLIPYVNKLLVSWEKDGSLKHIYQKFKLKPAKVKKE"
     gene            complement(20602..21357)
                     /locus_tag="VBC94_000980"
     CDS             complement(20602..21357)
                     /locus_tag="VBC94_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6979259.1"
                     /translation="MSLISYKNVNKYYGDYHALRQINLEIEPGQVVVLLGPSGSGKST
                     LIRTMNALESIDDGSLVVNGHELANISSKELVNLRKEVGMVFQHFNLYPHKTVLENIT
                     LAPIKVLKQSKKEAMEIAEKYLKFVNMWERKDSYPSMLSGGQKQRIAIARGLAMHPKL
                     LLFDEPTSALDPETIGDVLSVMQKLANDGMNMVVVTHEMGFAREVADRIIFMADGEIL
                     VDTTDVQDFFDNPTEPRAKQFLRNIINHTSTDL"
BASE COUNT         7042 a         3309 c         4412 g         6731 t
ORIGIN      
        1 aagataacgg ttacattttg ggaggtatta tgttaaatca attgaaaaac aactatttct
       61 ttgctgttat tcgagggaaa tcatctgagg atgcgctcga aatcgcaaag catgctattt
      121 tgggtggtat tcggaatatt gaagtaactt tttcgactcc tgaagcaagc aaagtcatca
      181 aacaattatc agatgacttt aaaaataata aagaaattat tgtgggggcc ggaacggtca
      241 tgacaactga acttgctaaa gaggctattg atgctggagc taaatttttg gttagcccac
      301 actttgattc agatattgct aatttagcta atgaaaacaa ggtttattat ttcccaggct
      361 gtgcaacggc aacagaaatc gtggtagcta gaaagtataa atgtcagatt ataaagcttt
      421 ttccaggtgg agttgtgggt tcaggtttta tcaaagatat tcatggtcct attccagatg
      481 ttgatttaat gccatcaggt ggcgtctctg tttcaaatgt agttgaatgg agaaaagcag
      541 gagcagttgc ggttggtgtt ggctcagcat tatctagtaa agtagccaca gaaggctatg
      601 atagtgttac taaaatagct aaacagtttg tttctgcact tgattagtga gtaagagaag
      661 aatatggcgt ttaatactga aacatttatg ttaaaaaatc aagcagctat acagctgtat
      721 gaagaggtca agagacaacc tatttttgat tatcattgcc acttagatcc aaaggacatt
      781 tttgaagatc atatttttga taatattgtg gatttgtggc ttggtggtga ccactataaa
      841 tggcgtctta tgagggctaa tggtatttct gaagcagaaa ttacaggtcc agcatccaac
      901 cttgagaaat ttaaagcatt tgctagaaca ctagaaagag catacggtaa tcctgtttat
      961 cattggtctg ctatggaatt gaaaaatatc tttggtgtta atgagatttt gacagaatca
     1021 aatgctgagg aaatttatca tcgtttaaat catttcttga aagagcataa aataagtcct
     1081 agacgtttaa ttgcagatag caaagttatg tttattggta ctacagacca ccctcttgat
     1141 actctcgagt ggcataaaaa gttggctgct gatgagagtt ttaaaacagt tgttgctccg
     1201 acatttaggc cagatgaagc ttttattgaa catcgacatt ttgttgattt tattactaaa
     1261 ctgggtgaca ttactcaaaa agagattaca gatttttcaa catttatagc agcaatggaa
     1321 gaacgcattg cgtattttgc tcaaaatggt tgtagggcta gcgatatcag ttttacggaa
     1381 atcgtctttg aacaaactga tgaattggag ttgaacgatt tatttaataa agtttgtgaa
     1441 ggctatatcc caaatcaatc agaaatcagc aaatggcaaa cagctgtttt tatggaactt
     1501 tgccgattat ataaaaaata tggttttgta acacaggtgc attttggagc tttgagaaat
     1561 aatcattcta ctatttttga aaaattggga gctgacgtgg gtgtggattc acttggtgac
     1621 caagtggctt taactgttaa tatgaaccgt ttattggatt ccttggttaa aaaagatagt
     1681 ctgcctaaaa tgatttggta taatctcaat ccagcttaca atatagcagt agctaacact
     1741 ttggcaaatt ttcaagctaa cgaacttgga gtgagatctt accttcaatt tggtgcaggt
     1801 tggtggtttg cagatactaa attagggatg attagtcaaa tgaatgcctt agcagagcaa
     1861 ggcatgttgg cgaattttat tggtatgtta acagattcta gaagttttct atcttatcaa
     1921 cgtcatgact attttcgacg tattttatgt acctacttag gagaatggat tgaagaagga
     1981 gaagttccag aagattatca agctttagga agtatggcta aagacatcgc ttatcaaaat
     2041 gcagttaatt attttaagaa ctaaaaagga agagtgaaat catggaaatg tcatttaggt
     2101 ggtatggtga agacgacccc gttactttag aaaatattgg tcaaattcca acaatgaaag
     2161 ggattgttac agcgatatac gatgtaccag taggagaagt ttggtcacgt gagcgcattc
     2221 aacagctgaa agaaaaggta gaggcagcag ggcttaaaat ttcggttatc gaatcagtac
     2281 ctgttcatga agatatcaaa ttgggacgtc caacacgtga tctcttgatt gataattata
     2341 ttcaaaccgt caaaaatttg gctgcggaag gtattgatac tatttgttat aattttatgc
     2401 cagtatttga ctggacacgc actgatttag catatcaata tccagatggt tccacagctt
     2461 tgatatttga tgagacggtt tctaaaaaaa tggatccagt aaacggtgaa ttatctttgc
     2521 caggatggga tgccagctat agtaaagaag aaatgaaggc tatcatggat gcctatgctg
     2581 aaattgatga ggaaaaactt tgggaaaatc tcacttattt tattaaacgt attattcctg
     2641 aagcggaagc cgtaggcgtt aaaatggcca ttcatccgga tgacccacca tattctattt
     2701 ttggtttacc tcgcattatt actgggttag aggctattga gcgttttgtg aaattgtacg
     2761 atagtaaaag taatggcatc actttatgtg tgggttctta cgcttctgat ccccagaatg
     2821 atgttttaga gatttcgcga cgtgcttttg agttagatcg tgtaaacttt gttcatgctc
     2881 gcaatattaa attaggtgat ggaaagtcat ttaaagaatc ggctcatcca tcagaatacg
     2941 ggtcaataga catgtatgaa gttattaagt tatgccatga atttgggttt gagggtgcta
     3001 ttcgacctga ccacgggcgt atgatttggg gagaaacagg tcgtccagga tatggcctat
     3061 acgatagagc tttaggagcc acttacgttt ctggtttata cgaagctgtg attaaaggtt
     3121 caaaataagg cgatgcttcc aaatttctcc tgttccaatc tcctgaaaga gaaatttgga
     3181 aagttattat cataatatga ttgtacttag ctaagagtag aatacttata gaagttaatg
     3241 agaggaataa gatgtcaaaa acaattacat ttaccaataa agttgtcgtc ataacaggtg
     3301 caggcggtgt tctctgtggt tatatggcta aagaatttgc gaaagcaggc gctaaagtgg
     3361 cattattaga tttgaatcaa gaagctgctc aaacatttgc tgatgaaatt gtagaagaag
     3421 gtggtatcgc taaggcttat aaagctaacg ttttgtctaa agaaaacctt gaagaagtgc
     3481 atcaagccgt tttagaggat cttggaccaa ctgatatttt agttaatggt gcaggaggca
     3541 acaatcctaa agctacaact gataatgaat ttcacgaatt agacttacct tcagaaacaa
     3601 aaaccttctt tgagttagat gaagctggta ttagctttgt ttttaactta aattatttag
     3661 gaacattatt gccaacgcaa gtatttgccc aagatatggt tggacgtgaa ggagcgaata
     3721 ttattaatat ctcttcaatg aatgctttca cccctctgac caaaatacca gcttattctg
     3781 gagctaaagc agctattagt aacttcacac aatggctagc tgttcatttc tcaaaagttg
     3841 gtatccgttg taatgctatt gcacctggtt tcttggtaac aaatcagaac cgaagcttgt
     3901 tatttactga ggacggtcag ccaactgcgc gtgctgagaa aattcttaat aatacaccaa
     3961 tgggaagatt tggagaagca agtgagctga ttggcggatt gttcttttta gcagatgaaa
     4021 aatcatcaag ttttgttaat ggcgttgtcc ttccgattga tggtggattt gctgcttact
     4081 ctggtgttta atttacaaga taatagccac ttaacacata agagattatt gagttgagtg
     4141 gttttttaag gaaatttgtt taatgatctt accactaaat gatgggtagg ttgctaattg
     4201 gaaaatcctc ataagttaga taaatttaga aactggagat aatatggtag atacaaatca
     4261 caaaatcgct ttttgcatag attctgatgg ttgcgcaatg gatacaatga catataagca
     4321 tcaactcttt tttggtccaa ttgctgcaga tatatttggg atatcagaac aagaaagaac
     4381 aagtttttta aaagagtggg atagagtcaa tctttattca agaactcgtg gagttaatcg
     4441 gtttgtggga ttggttatgg gccttgagtt tgccggagta gaacatattg ataaattaaa
     4501 agaatgggtc gaaacaacag actcattgtc caataattcc ttagagttag cgcttgccaa
     4561 cgattttagt agagatttag agaaggcttt agaatggtcc aatgaagtga atcgccaaat
     4621 caaaaattat gagggagatt ctttagcgtt ttcaggtgtg ttagaaagtt tagaacgatt
     4681 aagtcaattt ggtaatgttt atgtcgtgag ttctgctaac aaggaagctg tttggcaaga
     4741 gtgggaagaa cagggattgc taaagtatgt tgatgatatc tattgtcaag atagaggtaa
     4801 aaaagaagat gttatcgcca ctttaattgc aagtggttac tgccgtgaaa ctattttgat
     4861 ggtaggagat tccctgggtg atctggctgc agcagaacaa aatggtattg cattttaccc
     4921 agtacttgtt ggtaaggaag tcaaatcatg ggaaattctt agagaagata ttggtgaagc
     4981 ttttgcaaaa ggtcaattcg aacagcaaag gcaaaaagaa tctattaata ccttttgggc
     5041 taatttagat aactaataga aaggataggc tatgacacat ttagttgact taacaaagaa
     5101 acccttcaat ctaaatcaag aagcaattga gtggattgaa aaaacaatta atgagatgac
     5161 attggatgag aaaattgggc aacttttttt caatatggga gctagtcgtt cagaagaata
     5221 tttaacagat gttttggatc ggtatcatat tgctgctgtt cgctataata gaggtagttc
     5281 aagtgaaatc tatgatcaga atttaatttt acagacaaag tcgaaattgc cgatgttgat
     5341 tgctgcaaat acagaagctg gtggagacgg tgctgtcacg gatggaacta aagttggcga
     5401 tgaaataaaa gtggctgcta caaatgatcc gaaatatgct tatgaaatgg gaagaatcgc
     5461 agggatggag gcatctgctg tgggttgtaa tgcttcattt tctccaattg tagatttgac
     5521 acgtaattgg cgtaatccta tcatagctag tcgaaattgg ggtgctaacg ttgatcaaat
     5581 tatttcactt tcaaaagagt atatgaaagg aatcatgcaa tataatattg tacctttcgc
     5641 taaacatttt ccaggggatg gcattgatga gcgtgatcat catttgtcat ttgcttccaa
     5701 tcctatgagt aaagaggagt ggatgtcgac atttggccgc atttatggtg aattggctga
     5761 cgcaggttta ccaggagtca tggcaggtca catacattta ccaaacgttg aaaaagaaat
     5821 gcatccagag cgagaccttg atgatatgtt accagcttca ttgaataaaa cacttttaga
     5881 tgagttattg agaggagagt taggttataa tggagctatt gtaacggacg cttctcatat
     5941 ggttggaatg actgcttcaa tgccaaggag agatttgttg ccaacggcga ttgaagcagg
     6001 ttgtgattta ttcttattct ttaatgaccc tgatgaagac attcaatgga tgaaagaagg
     6061 ttacgaaaaa ggtattctaa cagaggaacg tctccatgac gctttacgtc gtactctcgg
     6121 tctaaaagcg aaattaggct tgcataacta tgagggacgt cgccaagaac tctttatgcc
     6181 taaagataag gctatggctt taatcaacac attagagtcg caaaaaatag ctgatgaagt
     6241 tgctgataaa gcagtaactc tggtaaaaga taaacaaaaa gatatctttc cagtaaatcc
     6301 agaacgttat cgtcatatct tacttgttaa cgttgaagga tataagggag gttttggtgc
     6361 gatgattgct ggaaacaagc aacgtgcatc ggattacatg aaagagttac ttgaggcacg
     6421 tggccatgaa gtaactgtct gggagtctac cgaagagcgt attatgaaat tgccacaaga
     6481 ggagcgggca gcagctatcg ctaatgttta cgcacaaaaa cagccaatcg caaatctaac
     6541 agaacactat gacttgatta ttaacttggt agatgtcaat gctggaggaa caacacaacg
     6601 tattatttgg ccagcagcta aaggcacccc tgatcagcct ttttacgttc atgaaatacc
     6661 atctattgtg atttcagtac aacatgcatt tgctttagca gatatgccac aggtagggac
     6721 ttatattaat gcttatgatg ggcttcctag cacgataagt gcagtagttg cgaaattggc
     6781 tggagaatca gaatttacag gagtttcacc agtagatgct tactgtggtt taattgatac
     6841 acatttatgg cgccaatgct aatagaaaca agatgatgta cttttatgta acataaaaaa
     6901 agagcttaaa gctctttttt tatccttcaa aatagagtag ttccttgggt gttttggtaa
     6961 atacttcaaa gccggtttta gtcacataac cgcagtcttc aattcgcaca ccaactttat
     7021 ctggtatata aatacctggt tcaacagaga agcacatgcc ttcttgaatc tccatatcgt
     7081 taccagccat aatagatgga aattcatgga catccatacc taatccatga ccaaggcggt
     7141 ggttaaagta ttgtccataa ccagcctttt caataacatt tcttgctgct gcgtcaactt
     7201 cagaagcaag gactcctggc ttaataaaat caatagccgc ttgatgtgct tcaagacata
     7261 aatggtaaat atcttttttg aattgatcag gtttccccac tgctactgta cgggtcatat
     7321 ctgacgtata acctagtgtt tctacaccta aatcaaataa taacaaagca ttattttcga
     7381 ttttattagt tcctgggata ccatgtggat ttgctgcatt attgccagtc aaaaccatag
     7441 tgtcgaaact cattttattg ataccttgtt ttttcatttc aaattcgatt tgagcgataa
     7501 tatcggtttc agtgttgttt agcgagatat tgtcaaatcc aacttgaaca gctttatcag
     7561 caaattctcc agcgactaac attttctcaa tttcatctcg tgatttaatt aggcgcatgt
     7621 tttgaatata aggtgttagg ttttcaaaat gaccatcaaa aacggtttgg agaccttgga
     7681 atttagtaac attaagatta tcaaattcgg ctagaacttt agaaacagaa aaggatggta
     7741 agttgctagc tattttttgc caaggatttt ctgaatctat gtaaccaaag acgggaaatg
     7801 gtacactttg ttttgcacgc gatacttcta gtgctggtac aaataaaata ggatcgcgat
     7861 cttcatagac aaataaaaac atttgtcttt catggggatc gcaaaagaaa cctgttaaat
     7921 agttgactgt cacaggatca gaaaagacag caagttcagc ttggactgaa tgtaagtgat
     7981 ggcgtatacg attaagtttt gacataataa cccttcttcc taaaaggaac ttagtctagt
     8041 taggctagtt tcttatgcct attatttttc caaaaaaata ggaaaaaatc aagtttagtt
     8101 agtattttga aagtttttca taaaaaagct ttataaaagc ttgaaagtgt ttccaaaagg
     8161 tgataaaata tcagtgaaag cgtcattttc aaatagaaag gaagttaaat caatatgaat
     8221 acagatgata cgattacgat ttatgatgtt gcccgtgaag caggtgtttc gatggcaact
     8281 gttagtcgtg tggtaaatgg aaataaaaat gtcaaggaaa atacacgtaa gaaagtcctt
     8341 gaagttattg atcgtcttga ctatcgtcca aatgctgtgg cgcgtggttt agcaagtaaa
     8401 aaaacgacaa ctgttggtgt tgtcattcct aatatcgcaa atagctactt ttcaatttta
     8461 gcgcgtggta ttgatgatat tgcagcaatg tacaaatata acattgtcct tgcttcaagt
     8521 gatgaagacg atgataaaga ggttaatgtt gttaatactt tgtttgctaa gcaagtagac
     8581 ggtattattt ttatgggaca ccacttaact gaaaagattc gtgcagagtt ctctcgttca
     8641 cgcacaccga ttgttttagc aggtactgtg gaccttgagc atcaattacc aagtgttaat
     8701 atcgattaca aagcagcagc agttgatgtt attgatattt tagctggcaa tcataaagac
     8761 attgcttttg tatcaggacc acttattgac gatattaatg gcaaggttcg tttggcagga
     8821 tacaaagaag gtctcaagaa aaatggctta aactttaaag aggggttggt ttttgaagcc
     8881 aactaccgtt atgctgaagg ctttgcatta gctcaacgtg tgattaatgc tggagcaaca
     8941 gcagcatatg ttgctgagga tgagttagcg gcaggtttgt taaatggtct ttttgaggca
     9001 ggtaaacgtg ttcctgaaga ttttgaaatt atcacaagca atgactcgcc aatcgcacaa
     9061 tatacacgtc ctaacttaac ttctatcagt caaccggtct atgatttggg tgctgttagc
     9121 atgcgtatgc taacaaaaat tatgcacaaa gaagagttgg aagaaaaaga aatagtgctt
     9181 aatcacggga ttgttaaacg tggcacaaca aataattagt gttagagtat gttatttgaa
     9241 aaatagtggt agaatggtag tagcttagcg cttaactttc tgatacttaa catgatatat
     9301 tgagaggatt agggtagctt agcttcctaa tcttttttta gaggtgtaat atgacaaatg
     9361 aattgataat gcaagctttt gagtggtatt tacctagtga tgggaatcac tggaagaaat
     9421 tagaggagtc tatatcagac cttaaaaaac ttggaattag taaaatctgg ttaccaccag
     9481 catttaaggg aactagcagt gatgatgtag gatatggtgt ttatgatctc tttgatttag
     9541 gagaatttga ccagaatgga acaattagaa caaaatatgg taggaaagaa gagtatctaa
     9601 agcttattaa gtcgttaaag gcaaatggca ttaaaccgtt tgcagatatc gttcttaacc
     9661 ataaagccaa tggtgatcat aaagaaaaat ttcaagtcat caaagtcaat cctgaaaatc
     9721 gtcaagaagc attaagtgaa ccctatgaga ttgaaggatg gacgggattt gatttcccag
     9781 gtagacaggg tgagtacaat gattttaaat ggcactggta ccactttact ggtttagatt
     9841 acgatgctaa aaataatgaa acagacatct ttatgattgt aggcgataat aaaggttggg
     9901 cagacgatga tttgattgat gatgaaaatg gtaatttcga ttatctcatg tacaatgaca
     9961 ttgattttaa gcatcctgaa gttataaaaa acttacaaga ttgggctaag tggtttattg
    10021 aaactacagg cattgaaggt tttcgtttgg atgctgtgaa acatatcgat tcttacttta
    10081 ttcaaacttt cattaatgat attcgtacaa aaataaaacc cgatttagag gtctttggcg
    10141 aatattggaa atctgaccaa acatcaatga aggactattt agaggcaaca caatttcaat
    10201 tttctcttgt tgatgtcacc ttgcatatga atttttttga tgccagtcac caaaaccgtg
    10261 attttgatat gcgaacaatt tttgatgata gtcttgttat tgataatcct gagtatgctg
    10321 ttacattcgt agaaaatcac gatacgcaaa gtggacaagc tctagaaagt agagtggaag
    10381 attggtttaa acctttagct tatggcttga tacttttacg ccaacaaggg actccatgtc
    10441 ttttttatgg tgattactat ggcattcaag gagaatttgg ccaaccatcc tttaaagagg
    10501 ttattgataa aatggctgag ctacgccaaa actatgtatt tggcgaacaa gtcgattact
    10561 ttacgcattc aaattgtatt ggttggactt gtcttgggga tgaggaacat aattcttgtt
    10621 tagctgtggt cctaacaaat ggtgatcagg gatggaaaca tatggaagta ggggaaattt
    10681 atgcaggcaa aacttttgtt gattatttag gcaattgtga acaagaggtt gttataggag
    10741 atgatggttg gggtgatttt ttagttgcat ctgcgtctat ctcggcttgg gtccctaaaa
    10801 tagaggaagc taactgataa atcaggtgaa aaaaaaggta aaatgagata taataagacc
    10861 tatgaaagtt ttactgtatt tagaggctga agaatacttg aaaaagtcag gtatcggtcg
    10921 tgctatcaaa catcaagaga aagctcttca aattgctggg atagattata ccactaatcc
    10981 tacagatgac tttgaccttg ttcatatgaa tacctatggt attagaagtt ggcttttgat
    11041 gagtaaagct aaaaaaactg ggaaaaaagt aattatgcat ggccattcca ctgaagagga
    11101 ctttagaaat tcttttattg gctcaaattt agtatccccg ctttttaaat ggtacctttg
    11161 ccgtttttat caaaaagcag atgccattat aaccccaact gattattcta aacaactaat
    11221 aaaagcatat ggcatcaaga aaccaatttt tgttttatct aacgggattg atctaagtcg
    11281 ctatcagagg tcagaaaaga aagaagctgc atttcgtcac tattttcact taagtaagga
    11341 tgataaagtt gttatggggg ccggtcttta tttcatgcgt aagggaattg accaatttgt
    11401 tgaagtagct gcaaaaatgc ctgatattcg ttttatttgg tttggagaaa caaataaatg
    11461 ggttattcct cgaaaggtta ggcaaatcgt taccaagcaa catccctcaa atgttacttt
    11521 tgctggttac ataaaaggtg atgtttatga aggtgccatg agtgctagcg atgctttctt
    11581 ctttccgagt cgtgaagaga cagaagggat agtagtttta gaggctttag ctagtcatca
    11641 gcatgtggtt ttaagagata tccctgtgta tcacggttgg gtgaccgaag acagtgttga
    11701 attagcgact gatgttgatg gttttgttga aaagttggat aaagtcttat ctggtaagag
    11761 tgataagatt aaagaaggtt atcgtgtggc tgagagtcga agtatcgaac gaattgccca
    11821 tgaattggca agtgtttatc aaaaagtaat ggagttgtaa gatgcgtata ggtctattta
    11881 cagatacata ctttccccaa gtttcgggag tttcaacaag tatccgaact ttaaaggaag
    11941 ggttggagaa ggaaggacac gaagtttata ttttcacaac taccgaccgt aatgtcaaac
    12001 gttttgaaga ccctacaatt attcgtttac caagtgtccc ttttatttct tttacagatc
    12061 gtcgagtagt ttatagaggt ttaatttctg cttataggat agctaaagac tacgaacttg
    12121 atattattca tactcaaaca gaattcagtt taggactttt aggaaaatta gttgcaaaag
    12181 ctttaagaat acctgttgtg catacttatc atactcaata tgaggattac gttggttata
    12241 tcgcaaaagg caaattgatt aagccaagta tggtcaaata tatcatgcgt acctacctaa
    12301 gtgatttgga tggtgtcata tgtccttctc gaattgttct caatcttcta gatggttacg
    12361 gtgtcaaaat tccaaaacaa gttattccga caggtattcc tgttgagaac tatcgtcgag
    12421 aggatatctc tgaagaaaca atcaagaatc tacgaacgga gcttggcctt gcggataatg
    12481 atacaatgct attgagttta tcacgggtat cttttgaaaa aaatattcaa gcaatcttga
    12541 tgcatttatc agctgtcgtt gatgaaaatc ctcacgtaaa attagtgata gttggagatg
    12601 gtccttatct ttcagatcta aaagaacttg ttcactcttt agaacttgaa aattcagtta
    12661 tttttacagg tatggttgag catagccaag tggctatcta ctataaagca tgtgatttct
    12721 ttattagtgc ttcaacaagt gaaacgcaag gattaactta cattgagagt ctggcgagtg
    12781 gacgtccaat cattgcgcaa agcaatcctt atctagatga tgttatttca gataaaatgt
    12841 ttggaactct gtacaagaag gaaagtgatt tggcagatgc tatcctagat gcaatagcag
    12901 agacacccaa aatgactcaa gaagcttatg aacagaaatt atatgagatt tctgctgaga
    12961 atttcagcaa gtctgtatat gctttttacc tagactttct gatttcacaa aaagctagtg
    13021 tgaaagaaaa agtctctctg acgataggaa ataaggatag ccattctact cttcgttttg
    13081 tcagaaaagc ggtatattta cctaagaaag tttttacctt tacaggaaga gcctctaaaa
    13141 aagtggtaaa agcacctaaa cgtcgtattt catctattcg agatttttta gattgagacg
    13201 aataaaatta gtaaatgtct tgaaattata aaaaatattg ttataataaa aaatgaagac
    13261 atgttaggtt ctaatagatt tcaagagcga gcactggttg gtggaaagag tgcaattaga
    13321 gaacttagta ctacttcctt gattttatgt aaacataaaa gggtggttcc cttatcgcta
    13381 ctagaggtct gacacaagta tactcgagtt tttgtgttgg ataaattgag gtggaaccac
    13441 gtagtcttta cgtccttttg gatgtaaagg ctcttttttt ataaaagaag tggttgcata
    13501 aaagaggcag attgcttttc taatgttata cttattatcc ctatctagga taatgtggaa
    13561 ttcaatcaaa ggtcaatccc tttgtttatt agtgaattaa tgtgtttagg aattggtatc
    13621 accctaacag ttattaagaa aaggagaaca agatgattaa aatcactttc ccagatggtg
    13681 caattcgtga atttgaatct ggcatcacaa catttgaaat tgcacaatca atttctaact
    13741 ctttagctaa aaaagcttta gctggtaaat ttaatggtca acttattgat acaacacgtg
    13801 ctatcgaaga agatggtagt attgagattg taacacctga ccacgaagat gctttaggag
    13861 ttcttcgtca ctcagcagcg catttatttg ctcaagctgc aaaacgcctt tttccagact
    13921 tatgtttagg tgtaggtcca gctattcacg atggttttta ctacgatact gataataaat
    13981 caggtcaaat ttcaaatgat gatcttcctc gtattgaaga agaaatgaaa aaaattgtta
    14041 aggaaaatca cccttgtatt cgtgaagaaa tcagtaaaga agaagcgtta gaacttttta
    14101 aagatgaccc ttataaagtt gaattaatta gtgaacatgc tgaagatggt ttaactgttt
    14161 atcgtcaagg tgaatttgta gacctttgtc gtggacctca tgtcccatca acaggacgta
    14221 ttcaagtctt tcatcttcta aatgtcgctg gggcttactg gcgtggtaac agtgataatg
    14281 ctatgatgca gcgtgtctat ggtactgctt ggtttgataa aaaagatttg aaagcatatc
    14341 ttaaacgccg tgaagaagct aaggaacgtg accaccgcaa attaggtaaa gaacttgatt
    14401 tatttatggt taatcctgaa gtaggtcaag gattgccatt ctggttgcca aatggagcaa
    14461 ctattcgccg cgaattggaa cgttacattg ttgataagga gattgcttca ggttatcaac
    14521 acgtttacac acctccgatg gcatctgttg aattttacaa gacttctggg cactgggacc
    14581 actatcgtga agatatgttc ccaacaatgg atatgggaga tggggaggaa ttcgttcttc
    14641 gtccaatgaa ctgcccacac catattgaag tttataaaca tcatgttcac tcatatcgtg
    14701 aattgccaat tcgtatcgct gaacttggta tgatgcaccg ttatgaaaaa tcaggggccc
    14761 tcacaggatt gcaacgtgta cgcgaaatga cactcaacga tgcccatatt ttcgttacac
    14821 ctgagcaaat taaagatgaa tttcttaaag ctcttaatct catagcagaa atttatgaag
    14881 attttaactt aacagattac cgtttccgct tatcttatcg tgacccagaa gataaacata
    14941 agtattatga taacgatgag atgtgggaaa atgcacaagc tatgctaaaa gaggctatgg
    15001 atgattttgg attagattat tttgaagcag aaggcgaagc agccttttac ggtcctaaat
    15061 tggatatcca ggtgaaaact gctcttggta atgaagaaac attatctact atccaactag
    15121 atttccttct tccagaacgc tttgatctta aatacatcgg tgcagatggt gaagaacatc
    15181 gtccaattat gattcaccgc ggtggtattt caacaatgga gcgatttaca gctattttaa
    15241 ttgaaactta caaaggtgcc tttccaactt ggcttgctcc acaacaagtt tctgttattc
    15301 caatttcaaa tgaagcccac attgattatg cttgggaagt ggctcgtgcc cttaaagatc
    15361 gtggtattcg tgctgaagtg gatgaccgta acgaaaagat gcaatataag attcgtgcgg
    15421 cgcaaaccca aaaaattcca tatcaattga ttgttggtga taaggaaatg gaagaaaaag
    15481 ccgtaaatgt gcgtcgctat ggaagtaaag cgactgaaac aaaatctata gaggaatttg
    15541 tagaaagtat tttagctgat attgcaagaa aatctagacc tgacgaagtt aaataaattt
    15601 tattagtcat tataaaaatc attaataaat gatgtgaact gattactgta tgatgcagaa
    15661 tcagtttttt agatgctaat tttttcttga tgtttaatta taagctaatt atagataaat
    15721 aatctcatat aacattcaca aaattagatt ttttggtata attaatatga ttgacacata
    15781 aaggaaaata gtattaataa aaagggggat ggagcattgc gaacagtatt agtagtgcaa
    15841 ggtgatgacg aaacaataga gttattaagg tcgtatttag agggagctct ttataaggtt
    15901 gtgatggcta gtgatggtga ggaggctttt tctttatttc agcaacatca gattgattta
    15961 gcaatcatag atattacgct acctaaaata gatggatatg agttgacgcg actcattcgt
    16021 caagatagtc agattcctat tattatgcta gcagctaaga caacagatat ggacagaata
    16081 ttaggattga atattggagc agatgatttt atcacgaaac cgtttaattc tttggaggtt
    16141 ttagctcgaa ttaattcgca actgcgtcgt tactatgaat tcaatagtct tgccaaacct
    16201 aaaaatcaat ttataaagat aggggaattg gaactagatg aggaacatgt tgaactaact
    16261 aaaaatggta aacatatcaa gttgactgct acagaattta agattcttca tatcttaatg
    16321 tcatcaccag gaaagattta tactaaaaca caactctatg agaagattaa tggtaggtat
    16381 ttagaaggtg atgagacaac gattatggtg catatttcta atatccgtga caaaatcgaa
    16441 gatgatagta aatatcctaa atatataaaa acacttagag gagtagggta taaaatcgaa
    16501 aaaccgcagt atcttgttta aaaacataat ggctagtgag agccattttt tagtgaagat
    16561 atttcttggt tttaagcttt cctttttgct ataataagta aaattggagg ggagagatat
    16621 ggcaaagacc gctcttaata ttccttttac agttgaaggg atagaaaatt attatcaaaa
    16681 tataaaaatg atactgtctt tagcagataa atggagcata cctaaagatg atctattact
    16741 ctttttaaag ggactcaagg ctattgatga tataaaagtt ggctctccta tttttaagta
    16801 tttttggact tctttaagtt tacaagcacc cctcaaagct ttagaattcg tgttagagca
    16861 ggctaaaatg ccaactgaat tgtcaggaga attatcagaa acacaatact tggtcgcaca
    16921 gttttcagat gagttagctc cgcatgatga tttctggata gccttatcac aagttattta
    16981 tgattctttt cctgggaata gtctagccga agatactgta ttaaatagga aactccatca
    17041 attccgctat ttgatttcta gtcagcaagc acagtatgtt cgacgatact ttaaagatgt
    17101 tggtatgact gatagagatg ctttagtaaa ctacctatca tgtttaagag aaccagatag
    17161 tatcgcctac tatgaatctg ctcgcttgca taataaacgt cggagaaatg gtgaaatctt
    17221 tggtttccca gatgatgagc cggttattaa tagtaagctt ttaatttcat tccatacgga
    17281 atttattatt gatgacaaag gaaacttttt aaacgagatt gacgcagaag taatcacacg
    17341 taatggcatt atcaacggtg ctagctttaa ttatgctttt aaaaataata cacgtcataa
    17401 agaattagat gttgatcctg taaagttaga cccaaaattt cgaaacgata tgactagagg
    17461 ttatagatcg ccaaatctat ctagaaggaa atggtttttc tttaaagaag aggattacga
    17521 ttgtagttat ttcaataaaa aaggatatta cgcatttgga aggcgtagtg ctaagcaatc
    17581 ggttgataaa caagtcaaat acttgaagaa ggctgtgcag aaaatgcgca tcaactgata
    17641 catatactat taaaagatac catgatacta tggtatcttt taatttgcgg tattaatgtc
    17701 aaatggtttt tattgaccac atatttttat aaacgagtgg tcaaaattga aaaagtgtct
    17761 agcttatgtt aaaatacaat tgaataatag aaagaaggtg ctgtatgatt aagaaaaata
    17821 aagttttttt aggagttctt ttagtccttg ttgttatcct tggaggtggt gtattatttt
    17881 accaatcaca attccaaaag acaactaatc aagcgttagc tattgcttat aaagatgcta
    17941 aagtggctaa gaaggatgtc atacatcaaa aaattgacaa agaatttgaa aactttagag
    18001 ggagctacga aattgaattc aataccaaaa gcgcagaata cagctatcat gtagatgcta
    18061 agactggaca aatccttgag cgggacatgg ataataatgg cttttcaaaa tcgacttctc
    18121 agtcaagttc atcgtcaagt caaaaatctc ataaaataag tcaagaagaa gccaaaaaga
    18181 ttgcatttaa agatgctaat atagaggaat cagaagtcag taatcttaag atcaaagaag
    18241 aaattgaaaa tggaaaatca gtttatgata ttgactttgt ggacctgaaa aataaaaatg
    18301 aagttgatta tcaaattgat gcagaaactg gaaaaatcat tgaacgttct agagatcata
    18361 tgaatgatta atttaaataa gatataaaaa aacgaaggtc taaaaggcct tcgttttaat
    18421 tactcagtag cttgctccca acgttttgaa agtttacgtg aaaaacttga gatgataaag
    18481 tttgtaatga aatagacagc agttataata gcgtagagtg taaaaacttg tccagcttcg
    18541 aaatatcttc ccattagaat ttgacttttt ccgaagattt cttgaatagc tataacagaa
    18601 taaagtagtg atgtatcttt tataaccgtt acaaactgag aaataatggc tggcaacatt
    18661 ttgcgaaaag cttgtggaaa aataataata atgaaaactt gcaaataagt gaatccttga
    18721 gaaagtccag cttctgtctg tcctttatca actccattta aacctccccg aataatttct
    18781 gctaaagcag cggaggtaaa aattgtaaac gaagtaatac cagcgctgac tgatttcatc
    18841 tggaatacta agaaaatgat aaaaatccaa agtagatttg gaacatttct gacaaattcg
    18901 atatagatac tagctattaa tttccagata ggatttttgc tatttctcat aattgctaat
    18961 agagttccaa aaaacattga tagtaaaatg gaaataaaag atatatataa ggttaaaccg
    19021 aacccttgaa ggagaaatag tatatttgtt agtgttaaga ctgatgacat atcaaacctc
    19081 cttaagaata agttttctta tttaattctt cttgttttct tgcccagctt gctagtggga
    19141 aacaaataac aaaatagaag atagcagctc ctgcaaaagc tgggatataa ttagtcgtat
    19201 cataagccca ggccttagct aaaaacatga tatcggctcc agaaatgatt gctacggtag
    19261 aggtattctt gattaggtta acaacttggt tggtcattgg tggtaaaatt gttctgatag
    19321 cttgaggcaa aatgataagt tgcatggttt gattagctgt gaatccttga gctaaagcag
    19381 cctctgtttg tcctttaggg acagcttcaa ttcctgagcg aataacttca gaaatatagg
    19441 caccatggta taggccaacg cacaaaacag cggtgaaaaa agcactaatc ataatatgac
    19501 cattactaat gattgctagt ccatagtaga caaaaaccat ttgaaccaaa agaggtgtgt
    19561 tttgaaaaac ttcaacatag atacgtgata tcaacttacc aactttactt ttagaagttg
    19621 ataaaccacc agagagaatt cctaatatca gagcaagtag gagtgcacca aacgaaatac
    19681 ctagagtata taagaagcct ttaaagaata ggtcaaaatg gttaaagaaa gcgccccacc
    19741 ttgaaatggc aaaaggactg atgtttaata aagtcataaa aaatcctccc tattcttttt
    19801 ttactttagc gggtttaagc ttaaattttt gataaatgtg ttttaaacta ccatcttttt
    19861 cccatgatac cagtaactta ttaacgtagg gaatcaagac tttgtcctgt ttactggttg
    19921 caataccgta ttcttgcgta ttgaaccctt cttttaatat ggttgtatga gggctaaggt
    19981 agccgctaag gatagattta tcaactgaaa aagcatcaat acgattagcg tacagcgata
    20041 tcgctaattc tgggaaactc cctaattgag catagctaaa ttttagcttg tgttctttag
    20101 catatttttc taaattgact tttgtagtgg caccttgagc cacaccaatg tgtttaccat
    20161 ccaattcttt tatagttttg atatgactat ttttacgcac caaaaatcca atttcatcgt
    20221 gataataagc tttagaaata ttatagttag cttttctttc gggcgtgata gtgtatgttg
    20281 cgattagaat atcaatttgc ccattgtcca tcagaggctc tctggtttgt gcagttgtcg
    20341 gtacaaaaac tggtttgacg cctaaggatt tggcaatttt tttggcaata tcaatttcca
    20401 ttccttcgta ttgattcgtc tcagcattat aataaccaaa gttaggcaca tcttgcttga
    20461 ctccaacttt aaggacaccc gattttttga tttctgataa ttgattagct gacgttgagt
    20521 tgggataaaa taacaggaaa atgaaaatac aggataatag atagagcgtt aatctttttc
    20581 gtctcagcat atttcgtcct cctaaaggtc agtactggta tggttgataa tgtttcttaa
    20641 aaattgtttg gcacgtggtt ctgtgggatt atcaaaaaag tcttgcacat ccgtagtatc
    20701 tactaaaatt tccccgtcag ccataaaaat aatacgatca gcaacttctc gtgcgaatcc
    20761 catttcatga gttacaacga ccatattcat tccgtcattt gccaattttt gcatcactga
    20821 aagaacgtct ccgatggttt cgggatcaag ggcagaggta ggctcatcaa agaggaggag
    20881 ttttggatgc atggcaagcc ctctagcaat agcaattctt tgtttttgac cacctgatag
    20941 catagatggg taggaatctt ttctttccca catattgaca aattttaaat atttttcagc
    21001 aatttccata gcttcttttt tagattgttt taatacttta ataggagcta atgtaatatt
    21061 ttctaacacg gttttgtggg gataaaggtt aaaatgttga aaaaccatac caacttcttt
    21121 acggaggttg accaattctt tagatgaaat atttgctaat tcatgtccat tcacgaccaa
    21181 actaccatcg tcaatagatt cgagagcgtt cattgttcta atgagtgttg atttacctga
    21241 tccagaaggt cctaatagaa caaccacttg ccctggctct atttccaaat taatctgacg
    21301 aagggcgtga taatctccat aatacttgtt aacattctta taagatatga gtgacataag
    21361 aacctcctaa aataacagtt tattgttagg ttacctaaca ttatagataa gtgtatcacc
    21421 tgtttgtgag cttgtcaata aattcagaat attaaagagt aggattgatt taaacattgc
    21481 tatttttaga gcgg
//
LOCUS       DATROQ010000030        20477 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.030, whole genome shotgun sequence.
ACCESSION   DATROQ010000030 DATROQ010000000
VERSION     DATROQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20477
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.030"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..951
                     /locus_tag="VBC94_000981"
     CDS             <1..951
                     /locus_tag="VBC94_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEO6979260.1"
                     /translation="NTIYNTLRTDKGYKVYEGYLYEITGEECEEALDLVIPKNIVFAD
                     TDTCGYTFLLNEDGTVYDDVTFYKFDDKYWLASHKALDSYLDNINFDYTVTDISDEYK
                     MLQIEGRYSGEIAQSFYEYDISTLNFRTLIEMTYKGEKGYLARFGFSGEFGYQFFLPS
                     SIFATFVSDVCEGIAEYGDELDRYLRFEVGQPITDIYQQEEYSLYEIGYSWNLDFTKE
                     EFRGRDSLLEHIRSATVKSVGFSTKEKLASGTPVLFDDQIVGKIFWIADEKDSSENYL
                     GLMIVNQTYAHSGVTFVTEDGQILKTQSSPYCIPESWNKE"
     gene            948..3143
                     /locus_tag="VBC94_000982"
     CDS             948..3143
                     /locus_tag="VBC94_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEO6979261.1"
                     /translation="MSVYVSGIGIISSLGKNYSEHKQHLFDLKEGISKHLYKNHDSIL
                     ESYTGSITSDPEVPEQYKDETRNFKFAFTAFEEALASSGVNLKAYHNIAVCLGTSLGG
                     KSAGQNALYQFEEGERQVDASLLEKASVYHIADELMAYHDIVGASYVISTACSASNNA
                     VILGTQLLQDGDCDLAICGGCDELSDISLAGFTSLGAINTEMACQPYSSGKGINLGEG
                     AGFVVLVKDQSLAKYGKIIGGLITSDGYHITAPKPTGEGAAQIAKQLVTQAGIDYSEI
                     DYINGHGTGTQANDKMEKNMYGKFFPTTTLISSTKGQTGHTLGAAGIIELINCLAAIE
                     EQTVPATKNEIGIEGFPENFVYHQKREYPIRNALNFSFAFGGNNSGVLLSSLDSPLET
                     LPARENLKMAILSSVASISKNESLSITYEKVASNFNDFEALRFKGARPPKTVNPAQFR
                     KMDDFSKMVAVTTAQALIESNINLKKQDTSKVGIVFTTLSGPVEVVEGIEKQITTEGY
                     AHVSASRFPFTVMNAAAGMLSIIFKITGPLSVISTNSGALDGIQYAKEMMRNDNLDYV
                     ILVSANQWTDMSFMWWQQLNYDSQMFVGSDYCSAQVLSRQALDNSPIILGSKQLKYSH
                     KTFTDVMTIFDAALQNLLSDLGLTIKDIKGFVWNERKKAVSSDYDFLANLSEYYNMPN
                     LASGQFGFSSNGAGEELDYTVNESIEKGYYLVLSYSIFGGISFAIIEKR"
     gene            3148..4359
                     /locus_tag="VBC94_000983"
     CDS             3148..4359
                     /locus_tag="VBC94_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylJ protein"
                     /protein_id="HEO6979262.1"
                     /translation="MSKFLFAPFMMNLGESQRLSKLANCLYEQGHDIHILGDNYYPFL
                     FNNDAYRYHHCVEDKSVYNSKRYDAFFSLSTDFNFLSEEEIERICSIERDLLRQEKFD
                     AVLTGYRLSIVTSCRLESIPLIWIISGATHISEIVENSEGILPNGKISKASKPQTKDF
                     IKRVITTYSTNVKTWNNYIKKYGGKPFNNALELFTGDLNLVTDYSLFYEFDKDSSYKT
                     IGPILIDNVGFSKCSQINQDNKTVLLSFGTSFKRDWVESFLKTLPRHYHYLLTSCGEA
                     INIPGSYIEIVDFIDFKTIDKEIYFAIIHGGQGTVYAMAAQGIPFIGIPFFNEQFWNI
                     KKFSKEKCAYLVKKPDVREIRKAISCLERDYDIYKSYMIRLSQGIKEEAEASLQKAEA
                     EIESFIRKRQS"
     gene            4328..4903
                     /locus_tag="VBC94_000984"
     CDS             4328..4903
                     /locus_tag="VBC94_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylK protein"
                     /protein_id="HEO6979263.1"
                     /translation="MNPLSEKDKVNYIMIYKNRQSTLENNLSGDRLMRYQQANRQVSK
                     DKLLTGSYFVYQCLKKLGFTNKICQESFSSVSSYLIGLPKGKISYSNSGDYHILTYAP
                     SGSTGVDIEKYKDRSEQTYQNYLGESVSSDMDSKLLFYKAWLQKEISYKCGKSIDITY
                     QTMIDGYIYGYAFDNTFKVEAVYLKELLPDN"
     gene            5246..5587
                     /locus_tag="VBC94_000985"
     CDS             5246..5587
                     /locus_tag="VBC94_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979264.1"
                     /translation="MSEFLSAKPIGHNDSSNTILRIIENTKYIHHYDGKVVVEIPPQK
                     EPIKVFFMDSRLHYLEFSDICRYVTVVLKNSTVDNITNLGLDNHIVYDNGDDMVDPLP
                     SIQHGLFLNDD"
     gene            5621..6136
                     /locus_tag="VBC94_000986"
     CDS             5621..6136
                     /locus_tag="VBC94_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979265.1"
                     /translation="MTFYKKTDKFGFRKSKVCRSLCGALLGTVAVVSLATASTEIHAD
                     EATTSPTTVTKVPQPVQADTTALNTSKTHSTQATTTPVEAKENKVVKSETVQSESRVM
                     PRDKVVERPETVKASVNSDVSQPITTTPPTINEKTVEIPNLAQDTKKVAPKVTVTPEV
                     VNQPKQLPKYE"
     gene            6188..8845
                     /locus_tag="VBC94_000987"
     CDS             6188..8845
                     /locus_tag="VBC94_000987"
                     /inference="COORDINATES: protein
                     motif:HMM:NF017709.1,HMM:NF018035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6979266.1"
                     /translation="MTKPQGAVAEKATPAVPKPQKVRVIVEFNKESILDYATEQKKTV
                     AQLNQADVEKKLQSIKQEQDKVLKNIEKSVHFDSSKVKRYDAIINGVALDIQAQEIEK
                     LKTIADVRRVYVSQEYVQTKPLLSSSGQLIGLPEVWNNSQYKGEGTVVAVIDSGVDFK
                     HQALKIKEPNRAKYNKTSIEKLIHEKNLKGKFYSEKVPYGYNYYDYNDNLKDSYGVMH
                     GMHVTGIVGANDDNQKLYGVAPNAQILAMKVFSDDQQNPTTFTDVWLKALDDAILLKA
                     DVVNMSLGTPAGFVHEGKDYPELEVIARACKAGIVIAVAAGNEGNITDGNTYGVKPLA
                     ENYDTALIANPALDDNTLAVASMENLKKHAHVLKFKDKKSGTEVTEVINLHVAPNASK
                     TIIGLAVDLGAEAPSELSKHFDLSGKIAMLEIPEDNKSNGFLEKVQAITKLNPAAILL
                     YNNAKVKDDLGSQLLVESEAAKFNIARITRSTYNNIKNNSNKIITILTERQAIDNSLA
                     GQLSSYSSWGPTPDLRLKPEITAPGGHIFSTVEDNQYADKSGTSMAAPQVAGAAAVLK
                     QYITDKKIPVDNAADFIKLLLMNTAQPIINKQSKDGKTPYFVRQQGSGAMNLAKALVT
                     TVVATVTGTNDNNADGKLELRELKEKKFKARILLRNFGKTNKTYIISSEAIADPVDEK
                     GFRTQNSEHLVSKKADAVTRKVTVEAGKTLAVDLDVDYSDAEALTRNNFLEGYLNLKD
                     TEGVADLHLPFLGFYGSWTEQKAIDAFEGISEIGNGDKKRRVQFYVNKETNKTSSTFT
                     TNGMLSLPIYNNTVFFSPNSPFYDKAGVRIAALRNMEYVQYSIIDPDTNKEVRVLGRS
                     HDVRKLYRLDYRNSFAMMPDSIWDGKIKD"
     gene            8891..12208
                     /locus_tag="VBC94_000988"
     CDS             8891..12208
                     /locus_tag="VBC94_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979267.1"
                     /translation="MNNKGVGGDGVQIYQYYIKMDNNKPYLSPKDKTTVEKLEDRWKK
                     ITFKVQDTGIGLKDVYLQSVKYVGGGNNNLDLITPPGFKKEDKKVEKPKLDRPPGIDL
                     PAPTSMRSFDYSTPPGTKPSKPKDSLSTPPGFPDLNTPPDEAPKDSKKDAIEDKSGAI
                     KYAKSLQLSFVDDPILASKVNGKILQVESDGKLVIPRNALSANQFDDTSLKIYRNNNR
                     NKEITITTDYFADTKYVNITAVDYLSNTTFEQLATGETVDYHAIVFSSFAAIKDKGGK
                     IYVNDKLQETSRIALKDKSVKIGIELPNDVRHIDSLSVRRLNEVKTVDNILKNDEQDI
                     NLSKTYQLKYNPTNRRLEFTINNINSSSEIMTTFKDGKMPELVEQKDVSLDINDMDMS
                     KFKTIRLGRKDSEFKGQLIAKTGTVELDMFFKQSQDPASIIKKIYLIQNGVPNELKKF
                     DSSFGLTESQIDGYYIYKDAINLKFKLTSGASLKVVYKGQEDPYSHQKEDMTKKGEQL
                     SHSTQANENTAKVTFANIDWSHYSKVTVNGKEVGKGSELPLTKGWTTFVLHKTENSLN
                     VKSLIMETGSVSKKVQQLPLSPRLSKNKHMRDMLLTMQKDSAYYETSDSLVLRINLTA
                     DTKLNFNAVKGASALTENMMMRQFAVAGPQDDPVSEHKYPSVFLLTPALLETASEATL
                     NGKEITASGIIGHIKDGDKSKHVEVKMVNENGDMLGTPVIIQGKDLTNRTKPLMSGRR
                     VLYAGKQYEFRAKLPLSRFNTWIRVEVVTEAGEKASIVRRMFFDQSVPELNTAVAKRD
                     LTSDTALIHIVAKDDSLKLKLYQDDSLLESVDKTGLYSFRNGVEITKDMTVPLEFGDN
                     IIKLSAVDLSNYRRNETLHIYRNRFDVKASQMTADKGAKVTVDMLMKHLVVPEMAGAY
                     TLTIDEAPNTNESGMLTNAKVSIHYVNGGVDKVDVPIKVVDLEAIRKAEEAHKADEAR
                     KAEEARKAEEAHKAEEVRKAEEAHKVEEARKAEEGHKTQEAPIVEEGYKVNNVHQTDT
                     TVKASDLPKTKTVSAVHMARTDNKQITSHQTHVEKQIKNTLPSTGDSKRGYYITGMAI
                     VMLSVLFSLAKKFKSKY"
     gene            12392..13477
                     /locus_tag="VBC94_000989"
     CDS             12392..13477
                     /locus_tag="VBC94_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M13"
                     /protein_id="HEO6979268.1"
                     /translation="MQRKSYLKSMSVLTLTACLISGYVVKDIAMLHAVSASEKKANNV
                     SPRENLYRAVNDNWLANTKLKQGQTSVNSFSEIEDKLKQLLVSDMAKMASGKIETTND
                     EQKKMVAYYKQGMDFKTRDKNGLKPLKPVLQKLEAVSSMKDFQSLAHDFVMSGFVLPF
                     GLTVETNARDNSQKQLVLRQAPALLESPDQYKKGNKEGEAKLSAYRTSAMALLKQAGK
                     SNIEDRKLVKQAIAFDRLLSEKTQVDQSKITAESETAAGRYNPESMETVHNYAKEFDF
                     KELIEKLVGPTNKAVNVEDKTYFKQVNDVINSKQLANMKAWMMISMLVDQSDFLGEQN
                     RQAASAFKNVASGLTQIESKEKMLTPN"
     gene            13498..14316
                     /locus_tag="VBC94_000990"
     CDS             13498..14316
                     /locus_tag="VBC94_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEO6979269.1"
                     /translation="MGDYYGKKYFGEAAKKDVEHMAKKIINVYKTRLKNNTWLSENTK
                     AMAIKKLDNMRLMIGYPEDYPDLYRQYQFDSKASFFENNDNYRKLSNKKTFEEFNQSN
                     QREHWQMSANAVNAYNDPNTNSIVFPAAIFQSPLYDKTKTVSQNYGAIGAIIGHEISH
                     SFDINGMKYDEKGNLHDWWTKEDLKHYKKKTQAMIDQWDGLKADGGKVDGKLTLAENI
                     ADNGGVMASLEALKTEKIQTIKNFLNHGQVFGVKKQPKNKVSPQFSQMFMHHMN"
     gene            14313..14423
                     /locus_tag="VBC94_000991"
     CDS             14313..14423
                     /locus_tag="VBC94_000991"
                     /inference="COORDINATES: protein motif:HMM:NF013590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979270.1"
                     /translation="MRANIPVRNFQEFYDAFGVKKGDSMYLKPEKRLTLW"
     gene            14455..15486
                     /locus_tag="VBC94_000992"
     CDS             14455..15486
                     /locus_tag="VBC94_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6979271.1"
                     /translation="MKNITKLSTVALSLLLCTACAASNTSTSKTQSHHPKQTKLTDKQ
                     KEEPKNKEAADQEMHPQGAVDLTKYKAKPVKDYGKKIDVGDGKKMNIYETGQGKIPIV
                     FIPGQAEISPRYAYKNLIERLSKKYKIYTVEPLGYGLSDIPTKPRTLENITKEIHTGL
                     NKIGVKNFYLAAHSLGGMYSLNYAKNYPEEVRGFIGMDTSTPWMEGEQKTKYDPESAK
                     WAMKLPDVDDKTNEQYLSIAKKINGNKSQRDEDERLGDNLGKLKNVKFPKGMPTKYFL
                     ADQSVEGIKLRQADFKGLKNWDQQHIDLSEDPKDASVEILKGSHNIFQTQYEHMAKAI
                     DGFIQSHQK"
     gene            15521..16540
                     /locus_tag="VBC94_000993"
     CDS             15521..16540
                     /locus_tag="VBC94_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO6979272.1"
                     /translation="MNKITKLSTVALALMLCVGCSANKDNQKTKTEQPKQEKSIAKQK
                     QEREAEKAMIAKGAIDLTKYKAKPIEDYGQKIDVGDGKKMNIYTMGQGKIPLVVFPGQ
                     AEISPRYAYKNLLDRLGKKYKIYTVEPLGYGLSDMTDKPRTIENITKEIHTALEKVGV
                     KNFYLMAHSLGGIYGLNYAKTYSNDVKGFIGMDTSTPNMEGDGTTGESSASTLKWAES
                     IPEVDKKTNEQYLSIGAKKSGNKTERDEDRRVSDNLHKMEKVKFPKGMPAKYFLANES
                     AEDIKMRKKIFPKLKSWEQQHIDLSEDPKDVSVETLDASHLIYHTQYKHMAKAIDEWI
                     QSHEK"
     gene            16575..17636
                     /locus_tag="VBC94_000994"
     CDS             16575..17636
                     /locus_tag="VBC94_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6979273.1"
                     /translation="MNKITTLSTIALTLMLCVGCSANKDNQKTKTEDPKQENVTANPK
                     EDKATAAKRMKLTKELEKYNKGTESFISNKQYQKYIKTPIKEYGQKVEVKGKKMNVYT
                     VGEGKVPIVFIPGQGTVTAKHQYHNLISNLSKTHKVVVVEPFGSGLSDVIDQPRNLAN
                     ITSDIHEALQKVGITGKYVIASHSIGGVYALKYISTYPKEVLGLIGLDTSTPGMEGGK
                     QVDFAAPVLKELPKIPKVSDDINAQFFAIGHKILNNSNMKEEAKNSSNMINESANYKI
                     PKGIPAMYLLARESDLGLQLRSLDIPIKGSWQEQHKELSEDPKEVSIHTLDGTHQIYD
                     TNLKEVIDYTNQFMKTFEK"
     gene            17760..18989
                     /locus_tag="VBC94_000995"
     CDS             17760..18989
                     /locus_tag="VBC94_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6979274.1"
                     /translation="MNLWTLSLAYLKRQKMKTVTLFLVFLTIGTCLISLMSIQHSLEK
                     NILTKQGKSIYLTSKEKAYWPEQAYEALKKAKMVESVEASLSIDVGSRLKSVSPYNSS
                     KEENQVTLAGYQSTEDLRAFQTKALVLKKGSHLAADNTKQVLVPLKLAQKNHLSVGNK
                     LRLGKENVTIAGIYDANSAKSKNTFNPNIDNTLIAQATLVRKISKQKGYQTVAVRLSD
                     KRLVDTVIQNIKQWPLDFGKLDVQTAKEFYGDSYRNIETLHRLVGRIILIVSLVAMAI
                     LVVMLTFWINNRIKETGILLAIGKTKFEIIGHYLIEVLLVAGAAFTLSIIGGVFLGKT
                     FAAGLLSQVNGGVSSQIVQNSSLIIDRIDNLAVSVGVMDVFRLYAQGALICLFAVVLS
                     SYSILKLQPKQILSRMS"
     gene            18999..19793
                     /locus_tag="VBC94_000996"
     CDS             18999..19793
                     /locus_tag="VBC94_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6979275.1"
                     /translation="MNLFKRSFLYVSRKKRKSITLFVCLWLVASTLISGIAVKNAGLT
                     AKKTFSRQTGSILHISSDSTDLVGDGYGSGEIPEKAIVNIASNPNVKRVNNNLMAYAG
                     LTSEKMVTRPNDKEQYKEQVLQVHGNSYSDTDPKYTAGMISLKGGRHITPKDQRVIIV
                     HQAFAKTNHLKVGDKLMFSKDPVRNSKDKTLVEATIVGIYKGKTVQKSNYPYEMLENT
                     AFSDTQLIKDLYGYSKGKAFYRQTTVYPKKNADIETLKSFIKKQSY"
     gene            19894..20022
                     /locus_tag="VBC94_000997"
     CDS             19894..20022
                     /locus_tag="VBC94_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000962057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6979276.1"
                     /translation="MLVVSIILVMMALLFWIGGRTHETGILLAIGKSKAIIIVSTQ"
     gene            20031..20351
                     /locus_tag="VBC94_000998"
     CDS             20031..20351
                     /locus_tag="VBC94_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6979277.1"
                     /translation="MIAIPAFALSVLISQFIGQKLADSLVYQASRTVRADFMKGFGNM
                     QLGADSDTDMFMQTIKTLNVQVKPEEVWLVILVGLAIVLFAIITTSLIIARYKPRGIL
                     SKLS"
BASE COUNT         7373 a         3174 c         3903 g         6027 t
ORIGIN      
        1 aatactattt ataatacatt gagaacagat aaaggttata aagtttatga ggggtattta
       61 tatgaaatta ctggtgaaga atgtgaagaa gccttagacc ttgtgattcc taagaatatt
      121 gtatttgcag atacagatac ttgtggctac acttttttac tcaatgaaga tggaacagtt
      181 tatgatgatg tgactttcta caaatttgat gataaatatt ggttggctag tcataaagct
      241 ttggattctt atttagacaa catcaatttt gactataccg taacagatat ttctgacgag
      301 tataaaatgc tgcaaattga aggaagatat tcgggagaaa ttgctcagtc attttatgaa
      361 tatgatattt caacacttaa ttttcgtact ttgatagaga tgacttataa aggtgagaaa
      421 ggttatcttg ctagatttgg tttttctgga gaatttggct atcaattttt cctaccatct
      481 tctatttttg ctacttttgt ttcggatgtc tgtgaaggta tagcagagta tggggatgaa
      541 cttgatagat atttaaggtt tgaagtggga caacccatta ctgatattta tcaacaagaa
      601 gaatattctt tatatgaaat aggttattct tggaatctag atttcacaaa ggaagaattt
      661 agaggtcgcg atagcttgtt agagcacatc agatcagcaa cagttaaaag tgttggattc
      721 tcaacgaagg aaaaactcgc ttcaggaaca ccagtgctat ttgatgacca aattgttgga
      781 aagatttttt ggatagcaga cgagaaagac tcttcggaaa attacctagg tttgatgatt
      841 gttaaccaaa catatgctca ttcaggagtt acttttgtaa cagaagatgg ccaaattttg
      901 aaaacacaat caagccctta ttgtatccca gaaagttgga acaaagaatg agcgtatatg
      961 ttagtggaat aggaattatt tcttctttgg gaaagaatta tagcgagcat aaacagcatc
     1021 tcttcgactt aaaagaagga atttctaaac atttatataa aaatcacgac tctattttag
     1081 aatcttatac aggaagcata actagtgacc cagaggttcc tgagcaatac aaagatgaga
     1141 cacgtaattt taaatttgct tttaccgctt ttgaagaggc tcttgcttct tcaggtgtta
     1201 atttaaaagc ttatcataat attgctgtgt gtttagggac ctcacttggg ggaaagagtg
     1261 ctggtcaaaa tgccttgtat caatttgaag aaggagagcg tcaagtagat gctagtttat
     1321 tagaaaaagc atctgtttac catattgctg atgaattgat ggcttatcat gatattgtgg
     1381 gagcttcgta tgttatttca accgcctgtt ctgcaagtaa taatgccgta atattaggaa
     1441 cacaattact tcaagatggc gattgtgatt tagctatttg tggtggctgt gatgagttaa
     1501 gtgatatttc tttagcaggc ttcacatcac taggagctat taatacagaa atggcatgtc
     1561 agccctattc ttctggaaaa ggaatcaatt tgggtgaggg cgctggtttt gttgttcttg
     1621 tcaaagatca gtccttagct aaatatggaa aaattatcgg tggtcttatt acttcagatg
     1681 gttatcatat aacagcacct aagccaacag gtgaaggggc ggcacagatt gcaaagcagc
     1741 tagtgactca agcaggtatt gactacagtg agattgacta tattaacggt cacggtacag
     1801 gtactcaagc taatgataaa atggaaaaaa atatgtatgg taagtttttc ccgacaacga
     1861 cattgatcag cagtaccaag gggcaaacgg gtcatactct aggggctgca ggtattatcg
     1921 aattgattaa ttgtttagcg gcaatagagg aacagactgt accagcaact aaaaatgaga
     1981 ttgggataga aggttttcca gaaaattttg tctatcatca aaagagagaa tacccaataa
     2041 gaaatgcttt aaatttttcg tttgcttttg gtggaaataa tagtggtgtc ttattgtcat
     2101 ctttagattc acctctagaa acattacctg ctagagaaaa tcttaaaatg gctatcttat
     2161 catctgttgc ttccatttct aagaatgaat cactttctat aacctatgaa aaagttgcta
     2221 gtaatttcaa cgactttgaa gcattacgct ttaaaggggc tagaccaccc aaaactgtca
     2281 acccagcaca atttaggaaa atggatgatt tttccaaaat ggttgccgta acaacagctc
     2341 aagcactaat agaaagcaat attaatctaa aaaaacaaga tacttcaaaa gtaggaattg
     2401 tatttacaac actttctgga ccagttgagg ttgttgaagg tattgaaaag caaatcacaa
     2461 cagaaggata tgcacatgtt tctgcttcac gattcccgtt tacagtaatg aatgcagcag
     2521 ctggtatgct ttctatcatt tttaaaataa caggtccttt atctgtcatt tcgacaaata
     2581 gtggagcgct tgatggtata caatatgcca aggaaatgat gcgtaacgat aatctagact
     2641 atgtgattct tgtttctgct aatcagtgga cagacatgag ttttatgtgg tggcaacaat
     2701 taaactatga tagtcaaatg tttgtcggtt ctgattattg ttcagcacaa gtcctctctc
     2761 gtcaagcatt ggataattct cctataatat taggtagtaa acaattaaaa tatagccata
     2821 aaacattcac agatgtgatg actatttttg atgctgcgct tcaaaattta ttatcagact
     2881 taggactaac cataaaagat atcaaaggtt tcgtttggaa tgagcggaag aaggcagtta
     2941 gttcagatta tgatttctta gcgaacttgt ctgagtatta taatatgcca aaccttgctt
     3001 ctggtcagtt tggattttca tctaatggtg ctggtgaaga actggactat actgttaatg
     3061 aaagtataga aaagggctat tatttagtcc tatcttattc gatcttcggt ggtatctctt
     3121 ttgctattat tgaaaaaagg tgataaaatg agtaaatttt tatttgctcc tttcatgatg
     3181 aatttaggag agagccaaag attatcaaaa ctggctaatt gtttatatga acaaggacat
     3241 gatattcata tccttggaga caattattat ccatttttat ttaataatga tgcttatcgc
     3301 tatcaccact gtgtagaaga taaatctgtt tataatagta agcgatatga tgctttcttt
     3361 tctctttcaa cagactttaa ttttttatca gaagaagaaa ttgaacgcat ctgttctatt
     3421 gaaagagact tgttacgcca agaaaaattt gatgctgtat tgacaggtta ccgtctatct
     3481 attgttacaa gttgtcgttt agaaagcata ccattaattt ggattatttc aggggcaacg
     3541 catatcagtg aaattgttga aaactcagaa ggaatattgc ctaatgggaa gataagtaaa
     3601 gctagtaagc ctcaaacaaa ggattttatc aaaagagtaa tcactactta ttcaacaaat
     3661 gttaaaacgt ggaataacta tattaaaaaa tatggtggca aaccttttaa taatgcttta
     3721 gaattattta ctggtgatct aaacttggta acagattact ctttgtttta tgaatttgat
     3781 aaagattcgt cctataaaac gataggacct attttgatag ataatgtagg tttttcaaaa
     3841 tgcagccaaa ttaatcaaga caataagact gtactgctca gctttggtac ttcatttaaa
     3901 cgagattggg tggaatcttt tttaaagaca ttaccaaggc attatcatta tttactgaca
     3961 agctgtggtg aggctataaa cattccagga agttatattg agatagttga ttttattgat
     4021 tttaaaacca tagataaaga gatttatttc gctattatac acggtgggca aggaacagtt
     4081 tatgcgatgg ctgcacaagg tattcccttt ataggtatcc ctttctttaa cgaacaattt
     4141 tggaatatta agaagttcag taaagaaaag tgcgcttatt tggtaaagaa acctgatgta
     4201 agagagatac gtaaagcaat tagctgtctt gaaagagact atgatatcta taaaagttat
     4261 atgataagac taagccaagg aattaaagaa gaagcagagg catctttaca aaaagcggag
     4321 gcagaaattg aatcctttat cagaaaaaga caaagttaac tatattatga tttataaaaa
     4381 taggcagtct acattggaga ataatttatc tggcgatcgg ttgatgagat atcaacaagc
     4441 aaatagacag gtaagcaaag ataaactgtt aacagggagt tattttgtgt accaatgctt
     4501 aaaaaagtta ggttttacca ataagatttg tcaggaatct ttttctagtg ttagtagtta
     4561 tttaattggt ttgccaaagg ggaaaattag ttactctaat tctggtgact atcatattct
     4621 aacctatgct cccagtggtt caactggggt tgatattgag aaatacaaag atagatcaga
     4681 acaaacctac caaaactacc taggagaatc agttagtagt gatatggatt ctaaattatt
     4741 attttataag gcatggttac aaaaagaaat ttcctataaa tgtgggaaat ctatagatat
     4801 tacctatcaa acaatgatag atggctatat ttatggttat gcttttgata atacttttaa
     4861 agtagaagcg gtttacttaa aagaattatt accagataac taactcgcaa catgttgttc
     4921 ctacatatgc atctataaaa agcactttga agtatttcaa gggcttttta taataccttg
     4981 ggtagtcttt aacaattatg gttagagttg atatgataaa ctgatcattt ccacatttta
     5041 tgagaaaatc agaaaataga ggtgtttttt tgtgataata attagaaata ataaaaactg
     5101 aaatattttc taatgtaaaa aaagataaaa gaacttattg ctgaaaacga ttttaactta
     5161 acatttgcca ttgacaagta cttagaggag tgctaaaatt tcgttagctg gttaacaaac
     5221 cggttaagta atgtgaggtt cttttatgag tgaattcctt tcagccaaac ctattggtca
     5281 taatgattca tctaatacta ttttaagaat tatagagaat acgaaatata ttcatcacta
     5341 tgatggaaaa gttgttgtcg aaattcctcc tcaaaaagag cctatcaaag tcttttttat
     5401 ggattcacgt cttcattatt tagaattttc agacatatgt cggtatgtaa ctgttgtttt
     5461 gaaaaatagt accgttgata atataaccaa tttagggcta gataatcata ttgtttatga
     5521 caatggtgat gatatggttg atccgttgcc gagtattcag catggattat ttcttaatga
     5581 tgattaaaga tgtagacaaa aatttagaaa cgaggaagcc atgacatttt ataaaaagac
     5641 ggacaaattt ggatttagga aaagtaaagt gtgccgcagc ctatgcggtg ctttattggg
     5701 tacagtagca gttgttagtc tagctacagc aagtacagag attcacgctg atgaagcaac
     5761 aacctcacct accactgtaa cgaaagtacc tcagccagtt caagcagaca caacagctct
     5821 aaatacctct aagactcaca gtacacaagc aactacaact cctgttgaag ctaaggagaa
     5881 taaggttgtc aaaagtgaaa ctgttcaatc tgagagccga gtaatgccta gagataaggt
     5941 agtagaaagg ccagaaacag taaaagctag tgttaactca gacgttagcc aacctattac
     6001 aaccacaccc ccaacaatta atgaaaaaac agttgagatt cctaacttag ctcaagatac
     6061 taagaaggta gctccaaaag taactgttac accagaagtt gttaatcagc caaagcagtt
     6121 gcctaaatac gagtaagaaa actcagagaa aggcaataag agtacagaag caactaaggc
     6181 agttactgtg actaaaccac agggggcggt tgctgagaaa gctactccgg ctgtgccaaa
     6241 gcctcaaaaa gtaagagtaa ttgttgaatt taataaggaa tctatcttag attatgcgac
     6301 agaacagaaa aaaacagttg cacagttaaa tcaagctgat gttgagaaaa aattacaaag
     6361 catcaagcaa gagcaagata aagttcttaa gaatattgaa aaaagtgttc actttgattc
     6421 aagcaaagtg aagcgttacg atgctattat taatggtgtc gcacttgata ttcaagctca
     6481 agaaattgaa aaattaaaaa cgattgctga tgttagaagg gtctatgtct cacaagaata
     6541 tgttcagaca aaacctttac tatcttcctc agggcaactt attggtttgc cagaagtttg
     6601 gaacaactcc cagtataaag gtgagggaac tgttgttgca gttattgatt caggggttga
     6661 tttcaaacac caagcactaa aaatcaaaga accaaatcgt gctaagtata ataaaactag
     6721 tattgaaaaa ctgattcatg aaaagaatct caaaggaaaa ttctattccg aaaaagtacc
     6781 ttatggttat aactattatg actataatga taacctcaag gatagctacg gtgtaatgca
     6841 tggtatgcat gtaacaggta tcgtaggagc taatgatgat aatcaaaaat tgtatggtgt
     6901 tgcaccaaat gctcaaattt tagcgatgaa ggtattttca gatgaccaac aaaatcccac
     6961 aaccttcaca gatgtgtggc taaaagctct agacgatgct attttactaa aagctgatgt
     7021 tgttaatatg agtcttggaa cacctgctgg atttgtacat gaggggaaag actatcctga
     7081 acttgaagtt attgcaagag cgtgtaaggc aggaatcgtc attgcagtag cagcaggtaa
     7141 tgaaggaaat atcactgacg gtaatactta tggtgttaag ccgctagcag aaaattatga
     7201 tacagccttg attgccaacc cagccctcga tgataatacc ttggctgttg cctctatgga
     7261 gaaccttaaa aagcatgctc atgttttgaa atttaaagat aaaaaatctg gaacagaagt
     7321 tacagaagtc attaatcttc atgttgcccc aaacgcttca aaaactatta ttggattggc
     7381 tgtagatttg ggagcagaag ctccttcgga attgtcaaaa cattttgatt taagtggtaa
     7441 aatcgccatg cttgaaattc cagaagataa taaatctaat ggatttttag aaaaagtaca
     7501 ggcgattact aagttaaatc cagctgctat cttgctttac aataatgcga aggttaaaga
     7561 tgatttaggc agtcaattat tagttgaatc agaagcagct aaatttaata ttgctcgcat
     7621 taccagaagc acgtataaca atatcaaaaa taatagcaat aaaattatta ctattttaac
     7681 agagcgtcaa gctattgata atagtttagc aggacaatta tcttcttatt cttcttgggg
     7741 acctacacca gacttacgtc taaaacctga gattacagct ccaggtggtc atattttctc
     7801 aactgtggag gataatcaat atgctgataa atcaggaaca tcaatggctg cacctcaggt
     7861 agctggggca gctgcagttt taaaacaata cattacagat aaaaaaattc ctgttgataa
     7921 tgctgctgat tttattaaat tactcttgat gaacactgca cagccaatta tcaataaaca
     7981 atcaaaagat ggcaaaacac cttactttgt gcgccaacaa ggtagcggag caatgaatct
     8041 tgccaaagct cttgttacga cagtcgttgc aactgtaaca gggactaacg ataataatgc
     8101 agatggtaag ttggagttac gtgaattaaa agagaaaaaa tttaaagcac gcattctact
     8161 tcgtaacttt ggaaaaacta ataaaacgta tatcatctca tcagaagcga ttgcggaccc
     8221 agttgatgaa aaaggatttc gtacgcaaaa ttcagaacac ctagtatcaa aaaaagctga
     8281 tgcagttaca aggaaagtta ctgtagaagc aggtaagaca ttagctgttg accttgatgt
     8341 ggattattct gatgcagagg ctttaactcg caataatttc ttggaaggtt atcttaactt
     8401 aaaagatact gaaggtgtag ccgatttaca cttaccattt ttaggttttt acggttcatg
     8461 gacagagcaa aaagctattg atgcttttga aggcatttct gaaattggta acggtgacaa
     8521 aaaacgtcgc gttcaattct atgttaataa agaaaccaac aagacctcat caacctttac
     8581 aactaacggc atgttgagtc ttccaatcta taacaataca gtatttttct ctcctaatag
     8641 tcctttctat gacaaagcag gagttcgtat tgcagctttg cgtaatatgg agtatgttca
     8701 atattctatt atagatccag ataccaataa agaagttcgt gtacttggta gatcacacga
     8761 cgttagaaaa ctttatcgcc tagattatcg taatagtttt gccatgatgc cagattctat
     8821 ttgggatggt aagattaaag attaaattgc aaaaggggat aaacaataca tttatcaaat
     8881 taaagtacag ttgaataata aaggtgtcgg tggcgatggt gtccaaattt atcaatacta
     8941 tatcaaaatg gacaacaata aaccttactt aagtcccaaa gataagacta ctgtagagaa
     9001 gttagaagat cgctggaaaa aaattacttt caaagttcag gatactggca ttggtttgaa
     9061 agacgtttat cttcaatctg ttaagtatgt tggtggtggc aataataatt tagaccttat
     9121 cacacctcca ggatttaaaa aagaagataa aaaagttgaa aaaccaaaat tagaccgtcc
     9181 accaggaatt gatttaccag caccaacttc aatgagaagt tttgattatt caaccccacc
     9241 gggaactaag ccaagcaaac ccaaagatag tttatcaact cctccaggtt tcccagattt
     9301 aaacacgccg ccggatgaag caccaaagga tagtaaaaaa gacgctattg aagataaatc
     9361 aggagcaatt aaatatgcta agtctcttca acttagcttt gttgatgacc ctattttagc
     9421 tagcaaagta aatggcaaaa tattacaagt cgaatctgat ggcaaattag tcattcctag
     9481 aaatgctttg tcagctaatc aatttgatga cactagtctt aaaatttatc gtaataataa
     9541 tcgcaataaa gaaattacta tcacaacaga ttattttgca gatacaaaat atgtcaatat
     9601 cacagcggtt gactatttga gcaatactac ttttgagcaa ttagctactg gtgaaacagt
     9661 agattaccat gccattgtat tttcaagctt tgctgctatt aaagacaagg gtggtaagat
     9721 ttatgttaac gataaattgc aagaaacttc tcgtatagcg cttaaagata aatctgttaa
     9781 gattggtatt gaattaccaa atgatgtcag acatattgat agtttatctg ttcgtcgttt
     9841 gaatgaggtt aaaactgttg ataatatctt gaaaaatgat gaacaagaca ttaatctcag
     9901 caaaacttac caattaaaat acaacccgac aaatcgtcgt ctagagttta ctattaataa
     9961 cattaactca agttcagaaa tcatgaccac tttcaaagat ggaaagatgc cagaattggt
    10021 tgaacaaaaa gatgtttctt tggatataaa cgatatggac atgagtaagt ttaaaactat
    10081 tcgacttgga cgaaaggatt ctgaatttaa gggacaactt attgcaaaaa ctggaacagt
    10141 tgaattagat atgtttttca aacaatctca agacccagct tcaattatta aaaaaatata
    10201 ccttatccaa aatggtgttc caaatgaatt gaaaaaattt gactctagtt ttggtttaac
    10261 tgaaagtcag atagatggat actatattta taaagatgca attaacctta aatttaaatt
    10321 aaccagtggt gcaagtctta aagttgttta taaagggcaa gaagatccat atagtcatca
    10381 gaaagaagat atgactaaaa aaggtgaaca gctcagtcat tcaactcaag ccaatgaaaa
    10441 tacagcaaaa gtaacctttg ctaatattga ctggtcacat tatagtaagg ttactgtgaa
    10501 tggaaaagaa gttggtaaag gtagtgagtt acctttaact aaaggatgga caacatttgt
    10561 attacataaa acagaaaatt cattaaatgt taaaagtttg attatggaga cgggtagtgt
    10621 aagtaagaaa gttcaacaac ttcctttaag tcctagatta tctaaaaata agcatatgag
    10681 ggatatgcta cttactatgc aaaaagattc agcgtattac gaaacaagtg acagtctagt
    10741 ccttcgaatt aatctcactg cagatactaa acttaatttt aatgctgtta aaggagcgag
    10801 tgctcttact gaaaatatga tgatgagaca gtttgcagtt gctggaccac aagatgatcc
    10861 tgttagtgaa cataaatacc catcagtatt tctcttaact cctgccttat tggaaactgc
    10921 tagtgaggca actctaaatg gtaaggaaat cacagcatct ggtattatcg gtcacatcaa
    10981 ggatggtgat aaaagcaagc atgttgaagt caaaatggtg aatgaaaatg gagacatgct
    11041 aggaacccct gttattattc aaggtaaaga cttgactaat cgaacaaaac cattaatgag
    11101 tggacgtaga gtactttatg ccggtaaaca atatgagttc cgggctaaat taccacttag
    11161 tcgttttaac acttggatta gggttgaagt ggtaacagaa gcaggagaga aagcaagtat
    11221 tgttcgtcgc atgttctttg accaatcagt tccagagctt aacacagcag ttgctaaacg
    11281 tgatttgact tctgatactg ctcttatcca catcgttgcc aaagatgact ctctaaaact
    11341 aaaattatat caagatgatt cattacttga atctgttgat aaaaccggtc tttatagttt
    11401 tagaaatggt gtagaaatca ctaaagatat gacagtacca ctagaatttg gagataatat
    11461 tattaagtta tctgctgttg acttatcaaa ttatcgtcgt aatgagaccc ttcatatcta
    11521 tagaaaccgt tttgatgtta aagcaagcca aatgacagct gacaaaggag ctaaagtaac
    11581 tgtggatatg ttgatgaagc acttagttgt tccagaaatg gcaggagctt atacattaac
    11641 aatcgacgaa gctccaaaca caaatgaatc aggaatgtta acaaacgcta aagtatcgat
    11701 tcattatgta aatggtggtg ttgataaagt tgatgttccg attaaagtag ttgacttaga
    11761 agctattcgt aaagctgaag aagcacataa agctgacgaa gcacgtaaag ctgaagaagc
    11821 acgtaaagct gaagaagcac ataaagctga agaagtacgt aaagctgaag aagcacataa
    11881 agtcgaagaa gcacgtaaag ctgaagaggg acataaaacc caagaagcac ctatagttga
    11941 agaaggctac aaagttaata acgttcatca aactgatact acagttaaag cgtctgattt
    12001 accaaagact aagacagttt ccgcagttca tatggctaga acagacaata aacagataac
    12061 ttcacatcag acacatgttg aaaaacaaat taaaaataca ttgccatcca ctggtgacag
    12121 caaacgtggt tattatatca ctggaatggc tatcgttatg ctgagtgtat tatttagttt
    12181 agctaaaaag tttaaaagca aatattaatg ataaaataaa atctatttag caacattaat
    12241 tgtgatggat aaaaggtttt atctcttaaa tcaaaagtag tagctcatta gctactactt
    12301 ttttgtataa gtgtaaaaaa gtgaattctt aacaagaatt taacacccta cttgctatac
    12361 taaaagtgaa taagaaagag aggtgtcatt tatgcaaaga aaatcctatt taaaatccat
    12421 gagtgttctt actttaacag cttgtcttat atcaggatat gtggttaaag atattgctat
    12481 gttacatgca gtatctgcca gtgagaagaa agcaaataat gtcagtccga gagaaaatct
    12541 ctacagggct gtcaatgata attggctagc caatacaaaa ctcaaacaag ggcagactag
    12601 tgttaatagt ttttcagaaa ttgaggataa attaaagcaa ctgttagtgt ctgatatggc
    12661 taaaatggcc tcaggaaaga ttgaaacaac caatgatgaa cagaaaaaaa tggttgcata
    12721 ctataaacaa ggtatggact ttaaaacaag agataaaaat ggtctcaaac ctctaaaacc
    12781 agttttacaa aaacttgaag cagtctcttc aatgaaagac tttcaaagtt tggcccatga
    12841 ttttgtgatg agtggttttg ttttaccatt tggtttgact gtggaaacca atgctcgaga
    12901 taatagccaa aagcaattgg tgcttcgtca agcacccgca ttacttgaat cacctgacca
    12961 atataagaag ggcaataaag aaggtgaggc taaattatca gcttaccgta cttcagcaat
    13021 ggctttgctt aaacaagctg gaaaaagtaa cattgaagat agaaaactag ttaaacaagc
    13081 tatagcattt gatagactct tatcagaaaa aacgcaagtt gatcaaagta aaatcacagc
    13141 tgaaagtgag acagctgcgg ggcgatataa ccctgaaagt atggaaacgg ttcacaatta
    13201 cgccaaggaa tttgacttta aagaattgat tgaaaaacta gttgggccaa cgaataaggc
    13261 agtcaatgta gaagataaaa cttattttaa acaggttaat gatgttataa atagtaaaca
    13321 attagccaat atgaaagcat ggatgatgat ttctatgcta gttgatcaat cagattttct
    13381 aggagaacaa aatcgtcaag cagcgagtgc ttttaagaat gttgcgtctg gtttgactca
    13441 gattgaatcg aaagaaaaaa tgcttacacc caattagaat atacttttgg ccatattttg
    13501 ggtgattatt atggtaagaa atattttggt gaggcagcta aaaaagacgt cgaacatatg
    13561 gctaagaaaa tcattaatgt ctataaaaca cggttaaaaa acaacacttg gttatcagaa
    13621 aatacaaaag caatggccat taagaaactt gataacatga gattaatgat tggctatcca
    13681 gaagattatc ctgatcttta tcgtcagtac caatttgata gtaaagcaag cttctttgaa
    13741 aacaatgata actacagaaa attatcgaac aagaaaacat ttgaagaatt taaccagtct
    13801 aatcaacgtg aacattggca aatgagtgcc aatgctgtaa atgcttataa tgatcctaat
    13861 accaattcca tagtctttcc agcagcgatt tttcaatcac cactctacga taaaactaaa
    13921 acagttagtc aaaattatgg agctatcgga gcaattattg gtcatgaaat ttcacactca
    13981 tttgatatta atggtatgaa atatgacgag aaagggaatc ttcacgattg gtggactaaa
    14041 gaagatttaa aacattataa gaaaaaaaca caagctatga ttgaccaatg ggatggcctt
    14101 aaagcagatg gcggtaaagt tgatggtaaa ttaactttag cagaaaatat tgcagataat
    14161 ggtggtgtta tggcatctct agaagctctt aagactgaaa aaatccaaac tataaagaat
    14221 tttttgaatc atgggcaagt atttggcgtc aaaaagcaac caaagaacaa agtaagtcct
    14281 caattcagtc agatgttcat gcaccatatg aattgagagc taacatccca gtacgtaatt
    14341 tccaagaatt ttatgatgcc tttggtgtta aaaaaggcga ttcaatgtat ctaaaaccag
    14401 aaaaacgttt gacactttgg taaagctatg ttttccagac aataaggaga atgcatgaag
    14461 aatataacaa agctatcaac tgttgcttta agcctactac tttgtacggc gtgtgctgca
    14521 tcaaacacgt ctacatctaa aacacagtct catcatccta aacaaactaa actcacagat
    14581 aagcaaaaag aagaacccaa aaacaaagaa gctgctgatc aagagatgca tccccaaggc
    14641 gctgttgatt tgacaaaata taaggcaaaa ccggtcaaag attatggtaa aaaaatcgat
    14701 gttggtgatg gcaagaaaat gaacatttat gaaactggtc agggaaaaat tccaattgtt
    14761 tttattcctg gtcaagctga gatttcgcca cgctatgctt ataagaattt aattgagcgg
    14821 ttaagtaaaa aatataaaat ttatacagta gaaccattag gctatggtct cagtgatatt
    14881 cctactaaac cacgtacgct agaaaacatt acaaaagaaa ttcacacagg cctaaataaa
    14941 attggagtta aaaatttcta ccttgcagct cactcacttg gcggtatgta ttctttaaat
    15001 tatgctaaaa attatccaga agaagtcaga ggatttattg gtatggatac cagtacacct
    15061 tggatggaag gggaacaaaa aacaaagtat gaccctgaaa gtgctaaatg ggcaatgaaa
    15121 ttaccagatg ttgatgataa gactaacgaa cagtatctgt caattgcgaa aaaaattaac
    15181 ggcaataagt ctcaacgaga tgaagatgag cgcttaggtg ataatcttgg taaattgaag
    15241 aatgtaaaat tccctaaagg gatgccaact aagtattttc ttgctgatca atctgtggag
    15301 ggaatcaagc tacgtcaagc agattttaaa ggtttgaaaa attgggatca gcagcatatt
    15361 gatttatctg aagatccaaa agatgcttcc gttgaaattc ttaaaggtag tcacaatatt
    15421 ttccaaacac aatatgaaca tatggctaaa gctattgatg gatttatcca atcccatcaa
    15481 aagtaatcgt tgaatgaaat acctaagtca aggagaaaaa atgaataaaa taacgaaatt
    15541 atcaaccgtt gccctcgctt tgatgctttg cgttggatgt tctgccaata aagataatca
    15601 aaaaactaaa actgagcagc ctaagcaaga aaagtcaatc gctaagcaaa aacaagagag
    15661 agaagcagaa aaagctatga ttgctaaagg tgctattgac ctaactaaat ataaagcgaa
    15721 accaattgaa gattacggac aaaaaattga tgtcggtgac ggtaaaaaaa tgaacatata
    15781 tacaatgggt caagggaaaa ttccgctagt agtcttccct ggtcaagctg aaatttcacc
    15841 acgttatgct tacaaaaact tgcttgatag attagggaaa aaatataaaa tttatacggt
    15901 tgaaccgctc ggttatgggc ttagtgatat gacagataag ccacgtacga ttgaaaatat
    15961 taccaaagag attcatacag ctcttgaaaa agtcggtgtc aaaaatttct atcttatggc
    16021 tcactcgtta ggtggcatat acggtctaaa ttatgctaag acatattcta acgatgtcaa
    16081 aggtttcatt ggcatggata caagcacacc aaatatggaa ggtgatggca caactggaga
    16141 atcttcagct tcaaccttaa aatgggcaga atccattcca gaagttgata agaaaaccaa
    16201 tgaacaatac ttatcaatcg gtgctaaaaa atcaggtaat aaaacagagc gcgatgaaga
    16261 taggcgtgtt agtgacaatc ttcataaaat ggaaaaagtt aagttcccta aaggtatgcc
    16321 agctaagtat ttcttagcta atgagtcagc agaagatata aaaatgcgta aaaaaatatt
    16381 ccctaagctt aaaagctggg aacaacaaca tattgactta tctgaagatc caaaagacgt
    16441 ttctgttgag acactagatg ctagccactt gatttatcat acgcaatata aacatatggc
    16501 gaaggctatt gacgagtgga ttcaatcgca tgaaaaataa ttattccccc caattatttt
    16561 actaaggaga aaagatgaat aaaataacga cattatcaac catcgccctg actttaatgc
    16621 tttgcgttgg atgttctgcc aataaagata atcaaaaaac taaaactgag gatcctaagc
    16681 aagaaaacgt aactgcaaat ccgaaggaag acaaggcaac agcagccaag cgaatgaagt
    16741 taacgaaaga acttgaaaaa tataataaag gcacagagag ctttattagt aacaaacaat
    16801 atcaaaaata tatcaaaaca cctatcaaag aatatggtca aaaggttgaa gtcaaaggca
    16861 agaagatgaa tgtctatact gttggagaag gtaaagtacc aattgtcttt attcctggtc
    16921 aagggacggt gactgccaaa catcagtacc ataatttgat tagtaattta agtaaaactc
    16981 ataaggttgt ggtagttgag ccatttggtt caggtttaag tgatgtcatt gaccaaccac
    17041 gaaatctagc taatattacc tcagatatcc acgaagcttt acaaaaagtt ggtattaccg
    17101 gaaaatatgt aattgcctct cattcaattg gtggtgttta tgcccttaaa tacatcagta
    17161 cttatccaaa ggaagtgcta ggattaatag gtttagatac atcaacgccg ggtatggaag
    17221 gtggtaagca agttgatttc gctgctccag tgttaaaaga attacctaaa attcctaaag
    17281 tatccgacga tattaatgca caattttttg ctattggaca taagatactt aataattcta
    17341 atatgaaaga agaagcgaag aattcttcta atatgattaa cgaaagtgcc aactataaga
    17401 ttccaaaggg aattcctgct atgtatttat tagcacgtga aagtgactta gggcttcaat
    17461 tacgtagttt agatatacca attaaaggca gttggcaaga gcaacataag gagttatcag
    17521 aagatccaaa agaagtctct attcatacac tagacggaac tcaccaaatt tatgatacta
    17581 accttaaaga agtaattgat tatactaacc aatttatgaa aacatttgag aaataattgt
    17641 ttttagaaac agtgctataa aatcactaat tgatagataa cgtagaagtc gatagagaat
    17701 atctaacgac ttctatcgtt attctagaga ttattaagat agaaaaagga gggaaaatag
    17761 tgaacctatg gacattaagt ttagcttatt tgaaacgtca aaaaatgaaa acagtaacct
    17821 tgttcttagt ttttctaact ataggcacat gtttaatcag tctgatgagt attcagcatt
    17881 ctttggaaaa aaatatccta acaaaacaag ggaaaagcat ttatttaacc tccaaagaaa
    17941 aggcttattg gcctgaacaa gcatacgaag ctttgaaaaa agcaaaaatg gttgagtcag
    18001 tagaggcaag tttaagcatt gatgttggta gccgtttaaa atctgtatca ccatataact
    18061 cttctaaaga agagaatcaa gttacattag caggttatca aagtacagaa gatttacgag
    18121 cttttcagac aaaagcattg gtcttaaaga aaggaagcca tttagcggcc gataatacaa
    18181 aacaagtact agttccattg aagttagcac aaaagaatca tttaagtgtg ggtaacaaat
    18241 tgagattagg caaagaaaat gttactattg caggaattta tgatgcgaat agtgctaagt
    18301 ctaaaaatac ttttaatccc aatattgata atacattgat tgctcaggca acattggtta
    18361 ggaagattag taaacaaaaa ggttatcaga cggttgcagt gcgcttaagt gataaacgtt
    18421 tagtggatac agttatccaa aacatcaaac aatggccgct tgatttcggg aaattagatg
    18481 ttcaaacagc taaagagttt tatggagatt cttatcgtaa tatcgagaca ttgcatcgtc
    18541 tagtgggacg aatcattctt attgttagtc tcgtggcgat ggcaatatta gttgtcatgc
    18601 taacattttg gattaataac cgcattaaag agacgggaat tttactcgct atcggaaaaa
    18661 ctaagtttga aatcataggt cattatctga tagaagtact gttagtagca ggtgctgcct
    18721 tcacactatc tattattggt ggtgtctttc ttggaaaaac atttgcagct ggccttttat
    18781 cacaagttaa tggtggtgtg agttcacaaa ttgtacaaaa tagtagtcta attattgata
    18841 gaattgataa cctcgctgtt tctgttggag taatggatgt ctttagactc tatgctcagg
    18901 gtgctttaat ttgcttattt gcagttgtat tatctagtta ttctattctt aaactacaac
    18961 caaagcaaat attgtcacga atgtcatagg aggttaatat gaatttattt aaacgttcct
    19021 ttttatatgt tagccgtaaa aagcgtaaga gtatcacttt attcgtatgt ttatggctag
    19081 ttgcttctac attaattagc ggtattgcgg taaaaaatgc tggtcttact gctaagaaga
    19141 cattttcaag gcaaactgga tcaattttgc atatttcatc ggatagtact gaccttgttg
    19201 gtgatggtta cggatctgga gaaatccctg aaaaagcaat agttaacata gcaagtaatc
    19261 caaatgttaa gcgcgttaat aataatttga tggcttatgc tggactaacc tcggaaaaaa
    19321 tggttacacg accaaatgat aaagaacaat ataaagaaca agttttacaa gttcatggta
    19381 atagttactc cgatacagat cctaaataca cagcggggat gatttctctt aagggagggc
    19441 gacatatcac gccgaaagat cagcgagtta taattgttca tcaggctttt gcgaagacaa
    19501 atcatcttaa agttggcgat aagttaatgt tttcaaaaga ccccgttaga aacagtaagg
    19561 ataaaacatt agttgaagca actattgttg gcatatataa aggtaaaacg gtacaaaaat
    19621 caaattaccc ttatgagatg ctagaaaata cagccttttc agatacgcaa ttgattaagg
    19681 atttatacgg ctattctaaa ggtaaggcct tttatcgcca aacaactgta tatcctaaaa
    19741 agaatgctga tatagaaact cttaaaagtt ttatcaaaaa acaatcctat tgattggaat
    19801 aaatatcaac tgacaattac agcaaccttg ctaacaagtt atgcaaaaag tatagatgtc
    19861 ttgaatcaat taattacttc tctacaaggc ggcatgttag tagtatctat tatcctagtg
    19921 atgatggctc tgctattctg gattggtggt agaacccatg agacaggtat tttattagct
    19981 attggtaagt ctaaagcaat tatcattgtc agtacgcagt agaagtacta atgattgcta
    20041 ttccagcctt tgctttatca gtacttatca gtcaatttat tggtcaaaaa ttagcagact
    20101 ccttagtgta tcaggcctct agaactgtca gagcagactt tatgaaaggg tttgggaata
    20161 tgcagcttgg ggctgatagt gatacagata tgtttatgca aacgattaag acgttaaatg
    20221 tacaagtgaa gcctgaggaa gtctggttag taattctagt tggtttagca atcgtcttat
    20281 ttgctattat tactactagt ttaattattg ctcgctacaa accaagggga atcttatcaa
    20341 aactgtccta aagtgccata agtataccga ccaatagtga catctatatt aacggttaaa
    20401 atgaatatgc tctgctattg aatacatagc tcaagaaaga gaaatctatg ctcgaattaa
    20461 aaaatattgc ttatcgt
//
LOCUS       DATROQ010000031        20241 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.031, whole genome shotgun sequence.
ACCESSION   DATROQ010000031 DATROQ010000000
VERSION     DATROQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20241
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.031"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            101..1030
                     /locus_tag="VBC94_000999"
     CDS             101..1030
                     /locus_tag="VBC94_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO6979278.1"
                     /translation="MNSLDNLLSHNLEDNRHLLPYEHMHTEVRNGYPDILFHWHPELE
                     ISYVHEGTARYHIDYDFFNSQSGDIILIRPNGMHSIHPIENKEHITDSIKFHLDLIGY
                     SIVDQVSLRYLQPLQTSSFKFIQCIKPSMTGYNDIKNCLFDIFNISKEENRHFELLLK
                     AKLNELLYLLYYHQYVIKKHTDDTYRKNERIRDLIDYINNNYQQNLTIEFLADYMGYS
                     KTHFMTVFKQHTGTSCTEFIIQVRLNKASEHLINSTTAIIDIANSVGFNNLSNFNRQF
                     KRYYHTTPRQYRKQFTNKKDGSTLVYRNINTKK"
     gene            complement(1087..2619)
                     /locus_tag="VBC94_001000"
     CDS             complement(1087..2619)
                     /locus_tag="VBC94_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEO6979279.1"
                     /translation="MENHKNEFQFSLESILGFVWRGIVVGLIAGFVVSIFRLAIEKIF
                     LVVMELYKSAHYQPIILLSITVTSIIAAVIIGFFIKSDPDIKGSGIPHVEGELKGMLS
                     PDWFSIVWKKFIAGILAISSGLMLGREGPSIQLGAMTGKGIAQYLNASRMEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLVWITALVASLVANFVSLNIFGLTPVLA
                     LPSELPSLNLNFYWIFLLMGLFLGILGFIYEWVILRFHVIYDYLGKLFHLPSHLYGIL
                     AVIFILPIGYYFPQLLGGGNGLIVSLPRSNLSLMMLGLFFLIRFLWSMLSYSSGLPGG
                     IFLPILALGSLAGAFFAVGMQYFGIISHQQISLFVVLGMAGYFGAISKAPLTAMILVT
                     EMVGDLKQLMAIGIVTMVSYIVMDLLKGEPIYEAMLAKMTFNPKDKVMTPTLIELTVS
                     DKISGKYVRDLELPENVLITTQIHHKTSAVVSGNTILNAGDTIFLVVNESEIKEVREQ
                     LM"
     gene            complement(2728..3801)
                     /locus_tag="VBC94_001001"
     CDS             complement(2728..3801)
                     /locus_tag="VBC94_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6979280.1"
                     /translation="MRRVYSFLGGIILVILILFGLTTYLEKKSSSTPNSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMHTKIKQGGTTYDIAVPSDYMIDKMIKEN
                     LLVKLDHSKIANWDAIGARFKNLSFDPKNKYSIPYFWGTVGIVYNDQLVKTPPKHWDD
                     LWRPEFRNKIMLVDSAREVIGVGLNSLGYGLNTKNISELKAASKKLDALTPNVKAIVA
                     DEMKGYMIQGDAAIGVTFSGEAREMLDGNKHLHYVVPSEGSNLWFDNIVIPKTVKHRK
                     EAYAFINFMMEPKNAAQNAEYIGYATPNLKAKALLPADIKNDKAFYPPDKTIDHLEVY
                     NNLGQKWLGIYNDLYLQFKMYRK"
     gene            complement(3794..4570)
                     /locus_tag="VBC94_001002"
     CDS             complement(3794..4570)
                     /locus_tag="VBC94_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6979281.1"
                     /translation="MKKFANIYLAFVFIILYIPIIYLIFYSFNKGGDMNSFTGFTFSH
                     YGELFQDSRLMLILVQTFFLAFLSALLATIIGTFGAIWIYQVRRRHQTSILSLNNILL
                     VAPDVMIGASFLLVFTVIGLQLGFTSVLLSHVAFSIPIVVLMVLPRLKEMNDDMINAS
                     YDLGASTWQMLKEVMLPYLSSGIISGFFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARRGISLEINALSTIVFLFSILLVIGYYYISKEKGEKNA"
     gene            complement(4567..5361)
                     /locus_tag="VBC94_001003"
     CDS             complement(4567..5361)
                     /locus_tag="VBC94_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6979282.1"
                     /translation="MKKTSSLFSIPYMAWLFLFVLAPVALIAWNSFFDINGHFTLANY
                     QTFFSSGTYLKMSFNSVLYAGIVSFITLLISYPAAYLLTKLKHKQLWLMLIILPTWIN
                     LLLKAYAFMGIFGQQGGINAFLTFIGIGPKQILFTDFSFLFVAAYIELPFMLLPIFNA
                     LDDIDQNLIYASDDLGANAWQTFQKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLITQNKGMGSTIGVILILVMVAIMWLTKERKA"
     gene            complement(5345..6499)
                     /locus_tag="VBC94_001004"
     CDS             complement(5345..6499)
                     /locus_tag="VBC94_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979283.1"
                     /translation="MTNPIIAFKNVSKVFEDSNTVVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLEASTGDIYLDGKRINDVPTNKRDVHTVFQNYALFPHMTVFENVAF
                     PLKLKKMDKKEIQKRVQETLKMVRLEGFEKRAIQKLSGGQRQRVAIARAIINQPKVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILSGKMIEDYLVEFNGKRFEAVDGGMRPNESVQV
                     VIRPEDLQITLPDEGKLQVKVDTQLFRGVHYEIIAYDDLGNEWMIHSTRKAIEGEVIG
                     LDFTPEDIHIMRLNETEEEFDARIEEYVDTDDHEDGLINAIEEERNEENL"
     gene            complement(6548..7450)
                     /gene="murB"
                     /locus_tag="VBC94_001005"
     CDS             complement(6548..7450)
                     /gene="murB"
                     /locus_tag="VBC94_001005"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEO6979284.1"
                     /translation="MIKTIQKELEGLDIRFDEPLKKYTYTKVGGPADYLAFPRNRLEL
                     SRIVKFANSQNIPWMVLGNASNIIVRDGGIRGFVIMFDKLSTVTVNGYVIEAEAGANL
                     IETTRIARYHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILLSAQVLTPQGELKT
                     IEARNMQFGYRHSVIQESGDIVISAKFALKPGDHLMITQEMDRLTYLRELKQPLEYPS
                     CGSVFKRPPGHFAGQLISEAHLKGQRIGGVEVSQKHAGFMVNIAEGSAQDYENLIEHV
                     INTVESTSGVHLEPEVRIIGESLL"
     gene            complement(7594..8082)
                     /gene="folK"
                     /locus_tag="VBC94_001006"
     CDS             complement(7594..8082)
                     /gene="folK"
                     /locus_tag="VBC94_001006"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEO6979285.1"
                     /translation="MTTVYLSLGSNIGDRETFLKQALFSIDHLQKTKVAQISAIYETA
                     AWGNTNQEDFFNICCQVETDLAPFELLDYCQEIEKRLKRVRHEHWGPRTIDIDILLFG
                     NQVINQEDLVVPHPYMTKRAFVLVPLLEIAPQLSLPNGSKLEDYLEKLNLGEVHYLKP
                     VE"
     gene            complement(8079..8441)
                     /gene="folB"
                     /locus_tag="VBC94_001007"
     CDS             complement(8079..8441)
                     /gene="folB"
                     /locus_tag="VBC94_001007"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEO6979286.1"
                     /translation="MDKIYLNKCRFYGYHGAFSEEQTLGQVFQVDAVLSLDLAKASQT
                     DDLIDTVHYGEVFDCIKNHVENEQYQLIEKLAGVIVEDIFLQFHPVQAITLKITKDNP
                     PINGHYESVGIELERKRV"
     gene            complement(8443..9246)
                     /gene="folP"
                     /locus_tag="VBC94_001008"
     CDS             complement(8443..9246)
                     /gene="folP"
                     /locus_tag="VBC94_001008"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEO6979287.1"
                     /translation="MKIGQYDITGKACIMGILNVTPDSFSDGGSYTTIDSALNQVGEM
                     LEQGVAIVDIGGESTRPGAVFVTAEEEIKRVVPMIKAIREVYPDLLLSIDTYKTEVAQ
                     AALDAGVHILNDVWSGLYDGKMLSLAAERNVPIILMHNQEEAVYQDIKKEVCEFLLER
                     AERALEAGVSKDNIWIDPGFGFAKTEEQNLELLKGLEQVCDLGYPVLFGISRKRTVNY
                     LLGGNREVTERDMGTAALSAWAIAKGCQIVRVHNVEVNKDIVTVISQLV"
     gene            complement(9250..9813)
                     /gene="folE"
                     /locus_tag="VBC94_001009"
     CDS             complement(9250..9813)
                     /gene="folE"
                     /locus_tag="VBC94_001009"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEO6979288.1"
                     /translation="MPNQEKMEKAIYQFLEALGENPDREGLKDTPKRVAKMYIEMFSG
                     LNQDPKEQFTAVFSENHEEVVIVKDIPFYSMCEHHLVPFYGKAHIAYLPNDGRVTGLS
                     KLARAVEVASKRPQLQERLTAQVAQALEDALAPKGIFVMIEAEHMCMTMRGIKKPGSK
                     TITTVARGLYKDDRYERQEILSLIQKG"
     gene            complement(9832..11094)
                     /locus_tag="VBC94_001010"
     CDS             complement(9832..11094)
                     /locus_tag="VBC94_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEO6979289.1"
                     /translation="MTYQEALEWIHSKLAFGIKPGLERMRWMLEQLGNPQNNLSAIHV
                     VGTNGKGSTTSYLQHIFTNSGYQVGTFTSPYIVDFRERISIDGQMIPESDFIKLVETV
                     RPVVERLHLETNLEPATEFEVITVLMFYYFGNSCPVDIVIIEAGMGGYYDSTNMFKAL
                     AVTCPSIGLDHQEVLGRTYVDIAEQKVGVLKKGVPFVYANDRQDVEEVFQIKAKETHS
                     QTYRLHNDFYIKEEENYFNYIGPQANIDHIQLQMPGHHQVSNASIAITTSLLLRDKYP
                     KLTLQTIKDGLEMTKWVGRTELIFPNVMIDGAHNNESVDALVQVIKKYQQKNVHILFA
                     AINTKPIESMLESLSSIAPVSVTSFDYPKSINLDKYPKAYTRVSDWKKWLHDINLTSD
                     KDFYVITGSLYFISQVRQELLLIKTTTT"
     gene            complement(11096..11983)
                     /gene="rarD"
                     /locus_tag="VBC94_001011"
     CDS             complement(11096..11983)
                     /gene="rarD"
                     /locus_tag="VBC94_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEO6979290.1"
                     /translation="MRKDNLGIILGLSAYVLWGLLSLYWKLLSGIEAYSTFAYRIIFT
                     VLTMLIYMLVSGRKTVYLKDLKGLVNNKKSFWTMFVASILISINWLVYIFAVTHGHAT
                     EASLGYYMMPIISILLSVLVLREHLARVVSLAILIAIMGVGILVYQTGHFPLISLTLA
                     LSFGFYGLLKKSISLSSDFSMLVESSFIAPFALIYIVFFAKDFLTDYNILQLVLLSLS
                     GIITAVPLLLFAEAIKRAPLNIIGFIQYINPTIQLLLALFIFKETIVSGEVIGFIFIW
                     LAILVFSIGQVHTMLKKGK"
     gene            complement(11970..12836)
                     /locus_tag="VBC94_001012"
     CDS             complement(11970..12836)
                     /locus_tag="VBC94_001012"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEO6979291.1"
                     /translation="MRIIVPATSANIGPGFDSIGVALSKYLSIEVLEESTEWLVEHNL
                     VNIPKDHTNLLIQTALHVKSDLAPHRLKMFSDIPLARGLGSSSSVIVAGIELANQLGN
                     LALSQKEKLEIATRLEGHPDNVAPAIFGDLVISSIVKNDIKSLEVMFPDSSFIAFIPN
                     YELKTSDSRNVLPQKLSYEDAVASSSVANVMVASLLKGDLVTAGWAIERDLFHERYRQ
                     PLVKEFGVIKQISTQNGAYATYLSGAGPTVMVLCSKEKEQPIVTELSKLCLDGQIQVL
                     NIERKGVRVEKR"
     gene            complement(12838..14121)
                     /locus_tag="VBC94_001013"
     CDS             complement(12838..14121)
                     /locus_tag="VBC94_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEO6979292.1"
                     /translation="MTIKIALLGFGTVAKGIPYLLKENQHKLLSLEGEDIVIDKVLVR
                     DNESRQRFINQGFTYNFVTEINTILQDPQIDIVVELMGGIEPAKTYLSQALGFGKHIV
                     TANKDLIALHGKELMDLADARGLALFYEGAVAGGIPILRTLSHSFASDKMTRLLGILN
                     GTSNFMLTKMFEEGWSYEQALKKAQELGYAESDPTNDVEGIDTAYKATILSQFGFGMP
                     IDFDDVNYKGISSIRSEDVEVAQEMGFAIKLVTDLRETPTGISVDVSPTLISQEHPLA
                     AVNHVMNAVFIESIGIGQSLFYGPGAGQNPTATSVLADIIDISRSIRSQIKIKPMNTY
                     HCPCRLSMQSDIFNEYYLAISLRNAEDSDTLGRYFEQENIGLKNVIEKALDDKQQEIY
                     VLTDEVSQEKITQFIEEFPESGVIQLINVFKVIGG"
     gene            14298..15185
                     /locus_tag="VBC94_001014"
     CDS             14298..15185
                     /locus_tag="VBC94_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO6979293.1"
                     /translation="MAHTPTSHRKPRKRSPWLAIASVFFLLIALIGIFLFFNNRSKQE
                     IKTKTNASSHRKIVTSIKKKKWVKQKTPVKIPILMYHAVHVMDPSEAASANLIVAPDI
                     FESHIKRLKKEGYYFLAPNEAYRALNENALPEKKVIWITFDDGNADFYTKAYPILKKY
                     KVKATNNIITGFVQEGRESNLNVQQMLEMKQNGMSFQGHTVTHPNLSLLTPELQTQEM
                     TLSKLFLDQKLSQDTLAIAYPSGRYNPTTLDIASQYYKLGLTTNEGVATKDNGLLSLN
                     RVRILPTTSDDDLIKTINQ"
     gene            complement(15208..16755)
                     /locus_tag="VBC94_001015"
                     /pseudo
     CDS             complement(15208..16755)
                     /locus_tag="VBC94_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
     gene            complement(16898..18274)
                     /locus_tag="VBC94_001016"
     CDS             complement(16898..18274)
                     /locus_tag="VBC94_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEO6979294.1"
                     /translation="MAYKTIYPYTNEVLHEFDNISDSDLEQSLDIAHALYKTWRKEDN
                     VEERQNQLHKVADLLRKDRDKYAEVMTKDMGKLFTEAQGEVDLCADIADYYADNGQKF
                     LKPVPLESPNGEAYYLKQAVGVLLAVEPWNFPFYQIMRVFAPNFIVGNTMLLKHASIC
                     PASAQAFEDLVREAGAPEGAFKNIFASYDQVSNLISDPRVAGVCLTGSERGGASIAAE
                     AGKNLKKSSMELGGNDAFLILDDADFDLLSKTIFFARLYNAGQVCTSSKRFIVMADKY
                     DEFVNMVVETFKSAKWGDPMDSETTLAPLSSAGAKDDVLKQIKLAVDHGAEVVFGNDT
                     IDHPGNFVMPTVLTNITKANPIYNQEIFGPVASIYKVDTEEEAIALANDSSYGLGSTV
                     FSSDPEHAKKVAAQIETGMTFINSGWTSLPELPFGGIKNSGYGRELSQLGFDTFVNEH
                     LVFTPNSD"
     gene            complement(18348..19355)
                     /locus_tag="VBC94_001017"
     CDS             complement(18348..19355)
                     /locus_tag="VBC94_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEO6979295.1"
                     /translation="MLFKEKIPGLILCFIIAIPSWLLGLYLPLIGAPVFAILIGIIVG
                     SFYQNRQLFNKGIAFTSKYILQTAVVLLGFGLNLMQVMKVGISSLPIIIMTISISLII
                     AYVLQKLFKLDKTIATLIGVGSSICGGSAIAATAPVINAKDDEVAQAISVIFLFNILA
                     ALIFPTLGNFIGLSDHGFALFAGTAVNDTSSVTATATAWDAINHSNTLGGATIVKLTR
                     TLAIIPITIVLSIYHMKQTQKEQSVSVTKIFPKFVLYFILASLLTTIVASLGFSLRIF
                     EPLKVLSKFFIVMAMGAIGINTNVSKLIKTGGKSILLGAACWLGIIIVSLTMQAILGT
                     W"
     gene            complement(19463..20149)
                     /locus_tag="VBC94_001018"
     CDS             complement(19463..20149)
                     /locus_tag="VBC94_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979296.1"
                     /translation="MKKFLKVFLTVLVLILIVGAGGLYFWNNRQSLEGKWRTVSLEKQ
                     VEKEIEQQLGSQAADMGISAADLVKGANMHMNVKNDEAKITVTAQIDEVKFHQAIKTF
                     IDKALEKQLKDQGLTYNDLSEAGKKIFDETKITDKQIDQQIDRSFQSAAQAVGGKYNT
                     NTGEMTLPVMDGKVHRLTSVIKVSHINKKANAFYGNIVKNGEKTAYKKEGSKLILGNE
                     KSYPFMKVTK"
BASE COUNT         6875 a         4060 c         3017 g         6289 t
ORIGIN      
        1 ttacactatc ctttcaaaaa ataatactat cctacgaaac ccattttttg atagttttct
       61 gttataataa tactatccta cgattttaga aagaaaaacg atgaactcac ttgataattt
      121 actatcacat aatttagaag ataaccggca tttattaccc tatgaacaca tgcatactga
      181 ggttagaaat ggttaccctg acattttatt tcactggcat ccggaacttg aaatttcata
      241 tgtccacgag gggaccgcac gttatcacat cgattatgat ttttttaata gccaatctgg
      301 tgatattatc ttgatccgtc caaatggtat gcattctatt catccgattg aaaataaaga
      361 gcatatcaca gatagcatta aatttcactt agatttaata ggatattcta ttgtagacca
      421 agttagcctt cgttatttac aaccgttgca gactagctct tttaagttta ttcaatgtat
      481 caaacctagt atgacaggat ataatgatat caaaaattgt ttgtttgata tttttaatat
      541 ttcaaaagaa gaaaatcgac attttgagct actcctaaaa gctaaattaa acgaactctt
      601 gtatttactc tattatcatc aatatgtcat taaaaaacat actgatgata cctacagaaa
      661 aaacgagaga attcgtgatc tgattgatta tattaataat aattaccagc aaaatcttac
      721 cattgaattt ttagctgatt atatggggta tagtaagaca cattttatga ccgtatttaa
      781 acagcatact ggaacatcct gtacagaatt tatcatacaa gttcgtctta ataaagctag
      841 tgagcatctt attaactcca ctacggccat tattgatatt gccaacagcg ttggttttaa
      901 taatctatca aattttaatc gccaatttaa acgctattat catacaactc ctcgccagta
      961 ccgcaaacaa tttaccaaca aaaaagacgg tagtacgctt gtctatagaa atataaatac
     1021 aaaaaaatag aaactagtta aatccatgac tagtttctat tttttattat ttaagcgtta
     1081 attagtttac atcagctgct cacgtacttc tttaatttcc gattcattca caactaaaaa
     1141 aatggtgtcg ccagcattca agatagtatt tcctgatact acagccgatg tcttatgatg
     1201 aatttgagtc gttattagca cattttctgg taattctaaa tctcggacat actttcctga
     1261 tattttatct gaaacagtta attcaatcaa tgtaggtgtc attactttat cttttggatt
     1321 gaaggtcatt ttagccaaca tcgcttcata aataggttct ccctttaata aatccatgac
     1381 aatataagat accattgtta caattccaat agccattaat tgttttaaat cgccaaccat
     1441 ttctgtaacc aaaatcatag ctgttagtgg tgcttttgag attgcaccaa aatagcccgc
     1501 cattcctaga acaacaaata aagatatctg ctgatgagat attataccaa agtactgcat
     1561 acctactgca aaaaaagcac ctgctaaaga tcctaaagct agaattggta aaaaaattcc
     1621 tcctggcaag ccactactgt aactcaacat actccataaa aaacgaatga ggaaaaataa
     1681 tcctaacatc atcagactaa gattactcct aggcagtgat acaattaagc catttccacc
     1741 ccccaataat tgtgggaaat agtaaccaat aggaagaata aaaattaccg ctaatatacc
     1801 atataggtga gatggtagat gaaatagttt acctaaataa tcatagatca catgaaatct
     1861 caaaattacc cactcataga taaagcctaa gatacccaaa aagagaccca tgagtaaaaa
     1921 aatccaataa aagttgagat ttaaagatgg taattctgat ggtaaagcta aaactggggt
     1981 taaaccaaag atattaagtg agacaaagtt tgccactaaa cttgctacca aagctgttat
     2041 ccaaactaag cgagaaaaat gatggtaaat ttcttcaact acaaataaaa ggcctgcaat
     2101 aggagcattg aaggctgctg aaagacctgc tgctgcacca ctagcaatta aaacacgttt
     2161 ttccattcga ctagcattga gatactgtgc aattccctta cctgtcatag ctcctaactg
     2221 aatactggga ccttcgcgac caagcataag tcccgaagaa atagctaaaa tacccgcaat
     2281 gaatttcttc catacaatac taaaccagtc tggcgatagc attcctttaa gttcaccttc
     2341 aacatgcggg atacctgaac ctttaatatc aggatcagat ttgataaaaa aacctattat
     2401 cactgcagcg ataatactag taaccgtaat gctcaataaa ataattggtt gataatgggc
     2461 acttttataa agttccataa caactaaaaa aattttttca atcgctaatc gaaatatgct
     2521 gacaacaaat cctgctatta agcctactac tattcctctc caaacaaatc ccagaattga
     2581 ttcaagtgaa aactgaaatt catttttatg attttccata ttttcctcct tttcttacaa
     2641 ttattttaac aaaaatatca caaactttct aggaactctt ggtaactaaa aatacctctc
     2701 atcagagaag tacttttagt taatagatta cttacgatac attttaaatt gtaagtacaa
     2761 atcattataa ataccaagcc atttttgacc caagttatta taaacttcta aatgatcaat
     2821 tgttttatca ggaggataaa atgctttatc atttttgata tctgctggta acagcgcttt
     2881 tgctttgaga ttaggggtcg cataaccaat atattctgca ttttgtgccg catttttagg
     2941 ttccatcatg aaattaataa aggcataagc ttctttgcga tgctttactg ttttggggat
     3001 tacaatatta tcaaaccaaa ggttcgatcc ttcactagga acaacgtaat gcaaatgctt
     3061 gttcccatca agcatttctc gtgcttctcc agagaatgta acaccaatcg ctgcatctcc
     3121 ctgaatcata tagcctttca tctcatcagc aacaatagcc ttaacgtttg gtgttagagc
     3181 atctaacttt ttagaagcgg cttttaattc tgatatgttc tttgtattca atccatatcc
     3241 caaggaattt aaaccaactc caataacctc acgtgcacta tcaactagca tgattttatt
     3301 tcgaaactct ggacgccata aatcatccca atgtttagga ggagttttaa ctaattgatc
     3361 attataaaca atcccaacag ttccccaaaa ataaggaatc gaatatttat ttttaggatc
     3421 gaaactcaaa tttttaaacc tagctccaat agcatcccaa ttagctatct ttgaatgatc
     3481 aagtttaacc agaaggtttt cttttatcat tttgtcaatc atataatcac taggtacagc
     3541 aatatcatat gtcgtcccac cctgcttaat tttggtgtgc atagcctcat tagagtcaaa
     3601 cgtttcgtat tgtacttcaa ttccagtttc ttttgtaaat ttttttaata aggctggatc
     3661 aatatagtct ccccagttat aaataactaa cttatctgag ttaggtgttg aactactctt
     3721 cttttctaag taagtggtta aaccaaagag gatcaggata acaagaataa taccgcctaa
     3781 aaaggaataa actctacgca ttcttttctc ccttctcttt cgaaatatag taataaccaa
     3841 ttaccaataa aatactaaat aaaaagacaa tagttgacaa ggcattaatt tccaatgata
     3901 taccgcgacg tgcgcgtgaa tagatttcta ctgaaagcgt tgaaaagcca ttacctgtaa
     3961 caaagaaagt aaccgcaaaa tcatctagcg agtaagtgaa agccataaag aaacctgaaa
     4021 taatacccga cgacaaatag ggtaacataa cttctttaag catttgccaa gttgaagctc
     4081 ctaaatcata ggaagcatta atcatatcat cattcatttc ttttaaacga ggtaagacca
     4141 ttaaaacaac aataggaata gagaatgcaa catggctcaa taatacagaa gtgaaaccta
     4201 actgtaaacc aataacagta aatactaaga gaaaacttgc accaatcata acatctggtg
     4261 ctactaaaag gatattatta agcgataaaa tagaggtttg atgtctacgt cttacctgat
     4321 aaatccaaat agctccaaag gtaccaataa ttgtagctaa taaggcgcta agaaaagcca
     4381 aaaagaatgt ttgcactaaa attagcatta agcggctatc ctgaaacagt tctccatagt
     4441 gactaaaggt gaatccagta aaagaattca tatccccacc tttattgaaa gaataaaaaa
     4501 tcaaataaat aataggaata taaaggataa taaaaacaaa tgctaaatag atatttgcga
     4561 attttttcat gctttcctct cctttgttaa ccacataata gcaaccatca ctaaaattaa
     4621 aataacccct atggttgagc ccataccttt attttgtgtg attaaaaaat gttgttctat
     4681 ggctgtccct aaagtaataa ctcgattacc accaataaga cgcgtaagca taaagagact
     4741 taaactagga ataaagacag attgtacacc tgctcttaca ccattcagag atagaggaaa
     4801 tataaccttc tgaaaagttt gccatgcatt tgcacccaag tcatcacttg cataaatgag
     4861 attttgatca atatcatcca aggcattaaa aatgggtaat aacataaacg gtaattcaat
     4921 gtaagctgct acaaaaagaa atgaaaaatc tgtaaataga atctgttttg ggccgatacc
     4981 tataaaggtt aagaaggcgt taattccccc ttgttgacca aatatcccca taaaagcata
     5041 ggcttttaac aaaagattaa tccatgttgg caaaataatt agcattagcc ataattgctt
     5101 gtgttttaat tttgtaagca aataagcagc tggatagcta atcaaaagcg ttatgaaact
     5161 aacgatgcct gcatatagga ccgagttaaa gctcatttta agataggttc ctgagctaaa
     5221 aaacgtctgg taattagcta aagtaaagtg cccattaata tcaaagaacg agttccaagc
     5281 aatcaaagca acaggtgcta atacaaataa gaataaccaa gccatataag gaattgaaaa
     5341 tagactagag gttttcttca tttctctcct cctcgatagc attgattaaa ccatcttcat
     5401 gatcgtcagt gtctacatat tcttcaatgc gcgcatcaaa ttcttcttct gtttcattaa
     5461 gacgcataat atgtatatct tctggagtga aatctaaccc aataacttca ccctcaatag
     5521 ctttacgagt tgaatgaatc atccattcat tacctaaatc atcataagca ataatttcat
     5581 agtggacacc tcgaaaaagt tgtgtgtcca ctttaacttg aagttttcct tcatcgggta
     5641 gggtaatttg aagatcttct ggacgaataa ctacttggac tgattcatta gggcgcatac
     5701 cgccatcaac agcctcaaag cgcttaccgt taaattcaac caaataatcc tcaatcatct
     5761 tgccagataa gatatttgat tcacctataa aagttgctac aaaatgattg ataggttcat
     5821 cgtagatatc tactggtgtt cctgattgaa caatttcacc ttcattcatg acgaaaatcc
     5881 aatcactcat tgccaacgct tcttcttgat catgtgtgac aaaaacaaag gtaattccta
     5941 aacgttgttg cagttcgcgc aattcatatt gcatctctgt acgcaatttc aaatccaatg
     6001 ctgataaggg ttcatctagt aagactactt taggttgatt gatgatagca cgcgctatcg
     6061 caacacgctg gcgttgtcct cctgataatt tttgaatagc tcgcttttca aacccttcta
     6121 aacgaaccat ttttagagtt tcttgaacac gtttttgaat ttctttctta tccatttttt
     6181 tcaactttaa tggaaaagca acgttttcaa aaaccgtcat atgtggaaac aaagcatagt
     6241 tttggaaaac ggtatgaaca tcccgtttat tagtgggaac atcattgatg cgtttcccat
     6301 ctaagtaaat atcgcctgta ctcgcctcta gcaaacctgc aataatattt aagatagttg
     6361 atttaccact tcctgaggcc cctaataagg tgtaaaattt accttcttca agttcaaaat
     6421 taatatcttt taagacaacc gtgttgctgt cttcgaatac ctttgataca tttttaaatg
     6481 caatgattgg gttagtcaat tctcataaat cctccatttt tctgactaaa gtaagtacta
     6541 aactacttta taatagcgac tcaccgataa tacgaacttc tggttccaaa tggacacctg
     6601 aagttgattc aacggtgtta ataacatgtt caataagatt ttcataatct tgagctgaac
     6661 cttctgcaat gttaaccata aaaccagcat gtttttgact aacctctact ccaccaatac
     6721 gttgaccttt aagatgtgct tcgctaatga gttgccctgc aaaatgtcct ggaggacgtt
     6781 tgaacacaga accacaagaa gggtattcta aaggttgttt cagctcacgg agataggtta
     6841 aacgatccat ttcctgagta atcatgagat gatctcctgg ttttaaggca aacttagctg
     6901 atataacaat atctccactc tcttgaatga cagaatgacg ataaccaaat tgcatattcc
     6961 tagcttcaat cgttttgagt tcaccctgtg gtgtcaaaac ttgtgcagac aataaaatat
     7021 gagcaatttc accaccgtaa gcgccagcat tcataaagac tgctccacct acacttccag
     7081 gaattccgca agcaaactca aagccagtta aactatgata acgtgcaata cgagttgttt
     7141 caatgagatt agctcctgct tctgcttcaa taacataacc gttgactgta acagttgaca
     7201 gtttatcaaa catgataaca aaaccacgga taccaccatc tctaacgata atattactcg
     7261 catttcctaa aaccatccaa ggaatatttt gactgttagc aaacttaaca atacgcgata
     7321 gttcaaggcg attccttggg aatgcaagat aatcagcagg tcctcctact ttagtataag
     7381 tatatttctt taaaggctca tcaaagcgaa tatcgagacc ttctaattct ttttgtattg
     7441 ttttaatcat aacctttatc tcaattctgt tttattgtgt tttatagaca caaaaaatcg
     7501 gcataaaacc gatttctatt ataccaaaaa tataggttgt tgacgataca ttatcacaat
     7561 ttctgtgtat tttaatttta aaatcctaac tgactattcg actggtttta aataatgaac
     7621 ttctcctaaa ttcaattttt cgagatagtc ttctagctta gaaccattag gtaaacttag
     7681 ttgaggtgct atttcgagta acggaactag aacaaatgct cgcttcgtca tatacggatg
     7741 gggtactact aaatcttctt ggttaatcac ttgattacca aaaagtaaga tatcaatatc
     7801 tattgtcctt ggaccccagt gctcatgtct cacgcgcttt aagcgttttt caatttcttg
     7861 acaataatcc aataattcaa aaggtgctaa gtctgtctct acctgacaac atatattaaa
     7921 aaaatcttct tggttagtat tcccccaagc agctgtttca taaatagcgg aaatttgcgc
     7981 gacttttgtc ttttgcaaat gatcaattga aaagagagcc tgcttcagaa aagtttctct
     8041 atcaccaata ttactaccta aactcaagta aacggttgtc atactcgctt gcgctccaat
     8101 tcaattccaa ctgattcata gtgcccatta attggtggat tgtcttttgt aatctttaat
     8161 gtaatagctt gtactggatg aaactgtaag aaaatatctt caacaataac acctgctaat
     8221 ttctctatta attgatactg ttcgttttca acatgatttt taatacagtc aaaaacttca
     8281 ccataatgaa ccgtatcaat taaatcatca gtttgtgatg cttttgctaa atcaagtgat
     8341 agaacagcat caacttgaaa aacttgcccc aatgtttgtt cttccgaaaa agcaccatga
     8401 taaccataaa accgacattt atttaaataa atcttatcca tattacacca actgactaat
     8461 tactgtcaca atgtccttat ttacttcaac attgtgaaca cgtacaatct gacacccctt
     8521 tgctattgcc catgccgata aagcagcagt ccccatatca cgttcagtta cttccctatt
     8581 accacctaga agataattaa cagtacgttt tcgagagatc ccaaataaca caggataacc
     8641 taaatcacac acttgttcca aaccttttaa tagctctaaa ttttgctcct ctgttttagc
     8701 aaaaccaaag ccaggatcta tccaaatatt atcctttgaa acacctgctt ctaaagcacg
     8761 ctctgctctt tcaagtaaga attcacaaac ttcttttttg atatcttgat aaacagcttc
     8821 ttcctggtta tgcatcaaaa taattggaac atttcgttct gctgctaaag atagcatttt
     8881 accatcatat aaacctgacc aaacgtcatt taaaatatgt acacctgcat caagggctgc
     8941 ttgagctacc tctgttttat aagtatctat tgataacaaa agatcagggt agacctctct
     9001 aatagcttta atcattggaa ctacacgttt aatttcttct tcagcagtca caaatactgc
     9061 acctggccgc gttgattctc ccccaatatc tacaattgcc accccttgtt caagcatctc
     9121 accaacttga tttaaagctg aatcaattgt ggtataagaa ccaccatctg agaaggaatc
     9181 aggcgttaca tttaaaatcc ccatgataca agctttacct gtaatatcat attgtcctat
     9241 tttcatcttt tatccttttt gtattagcga tagaatttct tgtctttcat atcgatcgtc
     9301 cttgtataaa ccacgagcta ccgttgtaat cgtcttactt cctggttttt taatgccacg
     9361 cattgtcatg cacatatgct ctgcttctat cataacaaaa atacctttag gcgctaaagc
     9421 atcctctaaa gcttgtgcaa cttgtgctgt taaacgttcc tgaagttgag gacgcttact
     9481 cgccacttca actgcacgtg caagcttact aagtccagta acacgtccat cgtttggtaa
     9541 ataagcaata tgagctttac cataaaaagg aaccaaatga tgttcacaca tggaatagaa
     9601 tggaatatcc ttgacaatca caacctcttc atgattttca gaaaagactg cagtgaattg
     9661 ctcctttgga tcttgattaa gtcctgagaa catttctata tacatttttg ccacacgctt
     9721 cggtgtatct ttcaaccctt ctcgatcagg attttctcct aaagcctcta aaaattgata
     9781 aatagctttt tccatttttt cttgattggg cattaattta tcctatcctt tttaagttgt
     9841 tgtagttttg attaatagta gttcctgacg aacttgagaa ataaaataaa gggagccagt
     9901 aatcacatag aaatctttat ctgatgtcag atttatatca tgtagccatt tcttccaatc
     9961 actaactcta gtgtacgctt taggatattt gtctaagttt attgatttag ggtaatcaaa
    10021 gctagtaacc gaaaccggag cgatactaga caatgattct aacatactct ctatcggttt
    10081 agtattaatt gcagcaaata gaatatgcac gtttttctgc tgatattttt ttataacttg
    10141 aacgagagca tcgacacttt cattattatg agcgccatca atcattacat ttggaaagat
    10201 taattctgta cggcctaccc attttgtcat ttcaagccca tctttaatgg tctgtagggt
    10261 taactttgga tatttgtctc ttaataataa actggtagtt atagctatgc tagcattgga
    10321 aacttggtga tgtcccggca tttgcaactg aatatgatca atatttgctt gaggacctat
    10381 atagttaaaa taattttcct cttcttttat ataaaagtca ttgtgtaaac gataagtctg
    10441 gctgtgggtt tccttggctt ttatttgaaa gacttcttca acatcctgac gatcattagc
    10501 atatacgaat ggaactcctt tttttaaaac acctactttt tgttcagcta tatctacata
    10561 agttcgtcct aaaacctcct gatgatctaa tccaattgac ggacaagtaa ccgctaaagc
    10621 cttgaacatg ttggtagaat cataataacc gcccattcca gcctcaataa tgacgatatc
    10681 aactggacag ctattgccaa aataatagaa catcaaaact gttataactt caaattctgt
    10741 agctggttct aggttggtct caaggtgcaa ccgttcaaca actggacgaa cagtctcaac
    10801 aagtttgata aaatcagact ctggaatcat ttgaccatca atgctaatac gctctctaaa
    10861 gtcaacaata tatggtgatg taaatgttcc aacttgataa ccagaatttg taaaaatatg
    10921 ttgtaaataa ctagttgttg atcctttacc attcgttcca acaacatgaa tggcagatag
    10981 gttgttttga gggtttccta actgctctag catccatctc atacgttcca aacctggttt
    11041 aataccaaaa gctaatttgc tatgaatcca ttctagcgct tcttgataag tcatcttatt
    11101 tacctttctt taacatcgta tgtacttgtc caatactaaa aactaaaata gcaagccaga
    11161 taaaaataaa acctattacc tccccactaa cgatagtttc tttaaaaata aacaatgcta
    11221 gcaataattg gatggttgga ttgatgtatt ggataaaacc gataatattt agaggagccc
    11281 tcttaatggc ttctgcaaat aataaaagag gaactgctgt aatgattcct gaaagtgaga
    11341 gtaaaactaa ttgtaatata ttataatcag ttaaaaaatc tttggcgaaa aacactatat
    11401 aaatgagtgc aaacggagca ataaaactac tttctaccag catcgaaaaa tcgctagata
    11461 aagaaattga tttttttaat aacccataaa aaccaaaaga aagcgctaga gttagtgaaa
    11521 ttaaaggaaa atgaccagtt tgataaacta aaattccaac tcccatgatt gcaatcaaaa
    11581 ttgcaaggct aactactctt gccaggtgtt cacgtaaaac aaggaccgac aacaaaattg
    11641 aaataatcgg catcatataa taaccaagac ttgcctcagt agcgtgacca tgcgtgactg
    11701 caaaaatata gaccaaccaa ttaattgaaa ttaaaatact ggctacaaac attgtccaaa
    11761 atgacttttt attattaact aagcctttta agtcttttaa atagactgtt tttctacccg
    11821 aaacgagcat ataaataagc atagttaaaa cagtgaatat aatcctataa gcaaacgtac
    11881 tatatgcttc aattccagat aataatttcc aataaagaga taaaagcccc cataaaacat
    11941 aggctgacaa gccaaggata attcctaaat tatcttttct caactctcac tccttttcgc
    12001 tcaatattaa gtacttgaat ctgtccatct aaacataatt tacttaactc tgtgacaatt
    12061 ggttgttctt tttctttcga acacagaacc attaccgtcg gtcctgctcc agacaaataa
    12121 gtagcatacg caccgttttg agtgctgatt tgttttataa ctccaaattc cttcaccaaa
    12181 ggttgacgat atctttcatg aaagaggtcc ctttctatag cccatccagc agttaccaaa
    12241 tcacctttca acaacgacgc aaccatcaca ttagcgacgc tgcttgaagc aactgcatcc
    12301 tcataactca atttttgagg tagtacattt cgactatcac ttgtttttaa ttcataattt
    12361 ggaataaaag cgataaagga actatctgga aacatcactt ccaaactttt aatatcattt
    12421 ttcactattg atgaaataac taaatcacca aaaatggccg gtgctacatt atcaggatgt
    12481 ccttctaatc gggtagctat ctctaatttt tctttttgag aaagagccaa atttcctaat
    12541 tgattggcta attctattcc tgcaacaatt acagaggaag aagaacctaa acctctggcc
    12601 aaaggaatat ctgaaaacat ttttaagcgg tgtggtgcca aatccgactt aacatgcaaa
    12661 gctgtttgaa taaggaggtt agtatgatct tttggaatat ttactaaatt gtgttcaact
    12721 aaccactctg tactttcttc taatacttca atgcttaaat actttgataa cgcaactcct
    12781 attgaatcaa agcctggacc aatattcgca ctagttgcag gtacaattat cctcatttca
    12841 ccctcctatt actttgaaaa cattgattaa ctgaatgaca ccactctcag gaaactcctc
    12901 aataaattga gttattttct cttggctaac ttcatctgtt aatacataga tttcttgttg
    12961 tttatcatcc aatgcttttt caataacatt tttcaaacct atattttctt gctcaaagta
    13021 ccttccaagt gtatcactat cttcagcatt tctcaaagaa atagctagat agtactcatt
    13081 gaagatatca gactgcattg acaacctaca cggacaatga taagtattca taggcttaat
    13141 ttttatctgt gatcgaatac tacgactaat atcgatgata tcggctaaaa cagaggttgc
    13201 tgttggattt tgtcccgcac ctggtccata aaaaagagac tgaccaatcc ctattgattc
    13261 aatgaatact gcattcatca catgattaac tgcagctaag ggatgctctt gagaaattag
    13321 tgtcggagaa acgtctacac ttataccagt tggagtttca cgaagatcag ttaccaactt
    13381 aatggcaaag cccatctcct gagctacttc aacgtcctct gagcgaatac tagaaatccc
    13441 cttataatta acatcatcaa aatcaatagg cataccaaat ccaaattgac ttaagatagt
    13501 ggctttgtag gcagtatcaa taccttcaac atcatttgta ggatcacttt cggcataacc
    13561 taactcttgt gcctttttta gagcttgttc ataagaccat ccctcttcaa acatttttgt
    13621 taacatgaag ttggaggtac cgttgagaat tcctaataaa cgtgtcattt tatctgaggc
    13681 aaacgaatgc gatagggtcc ttaaaatagg aatgcctcca gcaactgctc cctcatagaa
    13741 taaagctaga cctctagcgt ctgctaaatc catcaactct tttccgtgta aagcaatgag
    13801 atctttattg gctgtcacaa tatgtttacc aaatcctaat gcttgactca aataagtttt
    13861 agctggctca atccccccca ttaattccac tacaatatca atttgtggat cttgaagaat
    13921 agtatttatc tctgtcacaa agttataagt aaatccctga ttgatgaaac gctggcggct
    13981 ttcattatct cttactaata ctttatcaat tacaatatct tcgccttcta aagaaagtag
    14041 cttatgttga ttttctttta gcaaatatgg aataccctta gcaaccgttc caaaacctaa
    14101 taaagctatt ttaatagtca tgaatttctc cttagaaatt gtctctaact tcaagacaaa
    14161 cattggctat attataacaa attaccaata ttttttatag ggtaaacgcg tcttgtccta
    14221 tgaaagttct taaaaagaga atattatttc agaaaagatg atacaataaa agtactttaa
    14281 aagttaagga gaaataaatg gcacacacac ctactagtca tcgtaaacca cgaaaacgtt
    14341 ccccatggtt agctattgct tctgtctttt ttttgttaat agctttaatc ggcatatttc
    14401 tattcttcaa caatcgttca aagcaagaaa taaaaacaaa aacgaacgcc tctagtcatc
    14461 gaaaaattgt cacatctatc aaaaaaaaga aatgggttaa gcaaaagact cctgttaaaa
    14521 tacccatttt aatgtaccat gctgttcatg ttatggaccc ttctgaagcc gccagtgcta
    14581 acttaattgt ggcacctgac atttttgaaa gtcatataaa acgtctcaaa aaggaaggat
    14641 attacttctt agcccctaac gaggcatatc gtgcactaaa cgaaaatgct ttacctgaaa
    14701 aaaaagtcat ttggattact tttgatgatg gaaatgccga tttctacact aaggcatacc
    14761 ctatactcaa aaagtacaag gttaaagcca ctaataatat catcactggc tttgttcaag
    14821 aaggacgaga atctaacctc aacgttcaac aaatgctaga aatgaagcaa aacggcatgt
    14881 cattccaagg acatacggtt actcacccca atctttctct cttaactcca gaactacaaa
    14941 cccaagaaat gacactatca aaactgtttt tagatcaaaa actctctcaa gatactcttg
    15001 cgattgctta tccatcagga cgctacaatc ctacaacttt ggatattgct agtcaatatt
    15061 ataaactagg gcttacaaca aacgaaggag ttgcaacaaa agataacggt ttgctttctc
    15121 ttaatcgagt tcgtatctta ccaactacta gcgatgatga tttgatcaaa acgattaacc
    15181 aataaaaaga agccttctgg cttcttttta ttcttcataa gatttaaatg gttcattttt
    15241 acgtggataa cgatctggag aaatcgataa gcccatttct tttttctcat ggaaaagctc
    15301 cactaatagc gctagcatca tcaaaatcat tacgaatgaa aatggcagag cgacaataat
    15361 taaaacgttc tgcagcgcta acagaccacc agacaagagt aagacaatag caatcactgc
    15421 caacactagc ccccaaatta ctttagttcg attttttgga tttaaattac catcttctgt
    15481 caacatagcc aaaacgtacg ttgcagaatc tgctgatgta ataaaaaatg aaaaaattaa
    15541 aatgatagca atagtggata aaagccaacc taaagtgtag tgattaaaag tagcaaacaa
    15601 tacttcctct gttgcaaact ttgtcaaatt aatacccagt tgttgtacct gagttgataa
    15661 tgtgccaaat gctgcaaacc atacaaaact caataatgtc ggtattaaaa gaactaccgt
    15721 taaaaactcc cgaatgctac gccctttaga aatacgagcg atgaaaactc caacaaaagg
    15781 agaccaggaa atccaccacg cccaataaaa aatcgtccaa ttatcaatcc aagaacgttt
    15841 agtattgtca aaagcagctg cacgaaagct cattccaaag aaattttgta aataagagcc
    15901 taaagactct gttagggtat caaaaatacg aaccgttggt cccaatacaa taactaaagc
    15961 taagagggct accgctaaaa tcaaatttaa gttcgataaa attttaacac cttttcctaa
    16021 acctgataaa gctgacataa caaataaagc tgttgtaata aggataataa caatttgaac
    16081 aaatgcatta ttgggaacac ccaataaaaa acttaaccct ccgttgattt gagcagctcc
    16141 aaatccaagt gtcgtagcaa ctccaataac tgtagcaaca actgtggtga tatcaacaat
    16201 ttttcctagt caaccatctg tcttatcacc gaaaagaggc tttaaagtga cagacaagag
    16261 gtatttctct tgctttcgaa atccaaaata agctagagcc aaagcaacca atgcatatac
    16321 tgcccaagca tgtatccccc agtgaaaaaa tgtaaaacga aatgcatcag ctaatgctgt
    16381 ttgcgattcc ttaggtgcgc caggtgtcga aatagcaaaa tgcgataagg ggtcagctgc
    16441 tccgtagaag accaaaccaa tccccatacc tgctgaaaac atcattgcta tccatgatac
    16501 agttgaatat tcaggacgtg aatgaggtcg ccctaaacgt atttgtccaa gaggactcat
    16561 aataaaaaag acacacaaag caagaattga agtaactaac aacaagtaca accaaccgaa
    16621 attggtagat acctgttcac gcaagatttg tgtccaagat tgaaaaccac gaggaacaat
    16681 aattcctaat aagactaaaa taagcgatac aattagtgaa cctgtaaaca caggcgtaat
    16741 atgtttttta ctcaaaattt cctcctatac taaaatagta tagcatactt taataaatac
    16801 tcctctcatt ttaatcacct gtatcaagac ctctccttgt ctcctcaaca aaaaagaacc
    16861 agataacatt atctggttct tgtgtttgac ttagtgatta atcactattt ggtgtaaata
    16921 ccaaatgttc gttgacaaag gtatcaaatc caagttggct caattcacga ccatatcctg
    16981 aatttttaat acctccaaac ggtaattccg gtaatgatgt ccaccctgaa ttaataaatg
    17041 tcatccctgt ttcaatctga gccgccactt ttttagcatg ttctggatca gaagagaaaa
    17101 cagtacttcc taaaccataa ctagaatcat tagctaaagc aatagcttct tcttcagtat
    17161 ccactttata gatagaggct acggggccga aaatttcttg attatagatt ggatttgctt
    17221 tagtgatatt cgttaaaaca gttggcatga cgaagtttcc tggatgatct atagtgtcat
    17281 ttccaaaaac tacctcagca ccgtggtcta ccgccaattt aatttgtttt aaaacatcat
    17341 cctttgcacc tgcagatgac agtggagcca aggttgtttc cgaatccatt gggtcacccc
    17401 atttagcaga cttaaaggtc tcaactacca tatttacaaa ttcgtcatac ttatcagcca
    17461 taacaatgaa acgttttgaa gatgtacata cttgacctgc attatataaa cgcgcaaaga
    17521 atattgtttt actaagtaaa tcaaaatcag catcatctaa aataagaaaa gcatcgttcc
    17581 cacctaattc catagacgat tttttcaaat ttttaccagc ctcagctgct attgatgccc
    17641 caccacgctc agaaccagtt aaacacacac ctgcaacacg aggatctgat ataaggtttg
    17701 aaacttgatc atacgaagca aaaatatttt taaacgctcc ttctggagct cctgcttctc
    17761 ggactaaatc ctcaaatgct tgagctgatg caggacaaat agaagcatgt tttaaaagca
    17821 tagtattacc cactataaag ttaggggcaa atacacgcat aatctgataa aatgggaaat
    17881 tccatggctc gacagcaagt aaaacaccga cagcttgttt caaatagtaa gcctctccat
    17941 ttggactttc taacggaaca ggttttaaaa acttttgccc attatcagca taatagtcag
    18001 ctatgtctgc acacaagtcc acttctcctt gtgcttcagt gaaaagtttt cccatatctt
    18061 tagtcataac ttcagcgtac ttatcacggt ccttacgtaa taaatctgct actttatgca
    18121 attgattttg gcgttcttca acattatctt ctttgcgcca tgtcttgtag agggcatgcg
    18181 ctatgtctaa tgactgttcg agatcactgt cactgatatt atcgaattca tgcaatactt
    18241 cgttagtgta cggataaatt gttttatatg ccataatgtt ccctcctttt gatatcctca
    18301 ttataacatt tatgaaatcg attacaaata tttagcttta gacaagatca ccaagttcca
    18361 aggatagctt gcattgttaa actaactata ataatgccta accaacacgc tgcacctaaa
    18421 agaattgatt ttcctccagt tttaattagt ttgctgacat tagtatttat accaattgcc
    18481 cccattgcca taacaataaa aaatttagat aaaaccttaa gtggctcaaa gatacgtaac
    18541 gaaaaaccta atgaagcaac tatcgttgtt agcaaagaag ctaagataaa ataaagcaca
    18601 aacttcggaa atatcttagt aacacttaca gattgttctt tctgcgtttg tttcatatga
    18661 taaatagata atacaatcgt aattggaatg atggctaatg ttcgtgtcaa tttaacaata
    18721 gtagctccgc ccaaagtatt gctgtgattg atagcatccc atgcagtagc agttgctgta
    18781 actgacgagg tatcattgac agcagtccct gcaaataatg caaaaccgtg atcagagaga
    18841 ccaataaaat tccccagcgt tggaaaaatt aaagccgcta agatattgaa taaaaaaata
    18901 accgaaatag cttgtgctac ttcatcatct ttagcattaa taactggtgc agtcgctgca
    18961 attgcggatc caccacaaat agatgaacca acacctatta aagtagcaat cgttttatct
    19021 aacttaaata acttttgcaa aacataagca ataatcaatg aaatggaaat cgtcataata
    19081 atgatgggta acgatgatat accaactttc atcacttgca tgaggtttag tccaaaaccc
    19141 aataagacaa ctgctgtttg taaaatatat ttagacgtaa aagcaatacc cttattaaac
    19201 aattgtcgat tttgataaaa ggatccaaca attattccaa tcaagatagc aaagactggt
    19261 gctcctatta aagggagata aagcccaagc aaccaagatg gtatagcaat aataaagcat
    19321 aatattagtc caggaatttt ttccttaaat aacatgatag tcctcctcac attgactatt
    19381 atatcacaaa ctttaaaacg tggcaggaaa accgaaagag gaccagaata tgctctgatc
    19441 ctctttcatt actaattcac aattatttag ttactttcat aaatggatat gatttttcgt
    19501 tacctaaaat caattttgag ccctcttttt tataggctgt tttttcacca tttttcacaa
    19561 tattaccata gaacgcattt gcctttttat tgatatgcga tactttaata acacttgtta
    19621 agcgatgtac tttaccatcc ataactggca atgtcatttc accagtatta gtattatatt
    19681 ttcctccgac agcttgtgca gcactttgga aacttctatc aatttgttgg tcaatttgtt
    19741 tgtcagtgat ttttgtttca tcaaaaatct ttttacctgc ttcacttaaa tcattgtaag
    19801 tcaaaccttg atcttttaat tgtttttcaa gagctttatc aataaaagtt tttatagctt
    19861 gatggaattt tacctcatca atttgagcag tgactgttat ctttgcctca tcgtttttga
    19921 cgttcatatg catattagcg cccttaacca aatcagctgc actaatcccc atatccgctg
    19981 cctgacttcc aagttgttgt tctatttctt tctcaacttg cttctctaaa gaaactgtac
    20041 gccatttccc ctctagtgac tgacgattat tccagaaata gagaccgcct gcacctacaa
    20101 ttagaataag tactagtaca gttaaaaaaa cttttaaaaa ctttttcatg ataaaaactc
    20161 cttatgataa actacattaa tcataacata atatcacaac tccttcaatt aaaatagtaa
    20221 aataattata taaaattttg t
//
LOCUS       DATROQ010000032        20034 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.032, whole genome shotgun sequence.
ACCESSION   DATROQ010000032 DATROQ010000000
VERSION     DATROQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20034
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.032"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..118
                     /locus_tag="VBC94_001019"
     CDS             <1..118
                     /locus_tag="VBC94_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001920755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6979297.1"
                     /translation="KGNKLPATGENATPFFNVVALTIMSSVGLLSVSKKKED"
     gene            complement(624..773)
                     /locus_tag="VBC94_001020"
                     /pseudo
     CDS             complement(624..773)
                     /locus_tag="VBC94_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            830..1123
                     /locus_tag="VBC94_001021"
     CDS             830..1123
                     /locus_tag="VBC94_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEO6979298.1"
                     /translation="MSTVAVRVDDQLKDDATELFQSLGLDMSTAVKMFLIQSVKTQSI
                     PFEIKNKSSVSDEEFQNLVETKLKGIRVKASDPESVNAFFGDEDFSEYEEYFK"
     gene            1120..1308
                     /locus_tag="VBC94_001022"
     CDS             1120..1308
                     /locus_tag="VBC94_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, toxin component, MazF
                     family protein"
                     /protein_id="HEO6979299.1"
                     /translation="MSDIEVYSVLVSRIEYSDGTGSKVRPAVVVKFNDEVIKTLRFTT
                     KYENKSDNIRSQYLEVID"
     gene            1461..1832
                     /locus_tag="VBC94_001023"
     CDS             1461..1832
                     /locus_tag="VBC94_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEO6979300.1"
                     /translation="MKKKLLGLMILAISTVFLVACSTNSLNVKYYKVYDGEKMLVLEI
                     NDESGIYHNEGNFVVTNIDTKSKSFVLKGGSAKYTVLYDVKSDGKLNVDLGNFYYNAS
                     GKETYYKEGSKALEKALRDKE"
     gene            2203..2517
                     /locus_tag="VBC94_001024"
     CDS             2203..2517
                     /locus_tag="VBC94_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO6979301.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            2533..2919
                     /locus_tag="VBC94_001025"
     CDS             2533..2919
                     /locus_tag="VBC94_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO6979302.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            2948..4333
                     /locus_tag="VBC94_001026"
     CDS             2948..4333
                     /locus_tag="VBC94_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6979303.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSMMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            4336..4488
                     /locus_tag="VBC94_001027"
     CDS             4336..4488
                     /locus_tag="VBC94_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6979304.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            4511..5716
                     /locus_tag="VBC94_001028"
     CDS             4511..5716
                     /locus_tag="VBC94_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO6979305.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            5759..5980
                     /locus_tag="VBC94_001029"
     CDS             5759..5980
                     /locus_tag="VBC94_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979306.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            6097..6594
                     /locus_tag="VBC94_001030"
     CDS             6097..6594
                     /locus_tag="VBC94_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEO6979307.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            6569..7075
                     /locus_tag="VBC94_001031"
     CDS             6569..7075
                     /locus_tag="VBC94_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6979308.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            7059..9506
                     /locus_tag="VBC94_001032"
     CDS             7059..9506
                     /locus_tag="VBC94_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6979309.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            9509..11686
                     /locus_tag="VBC94_001033"
     CDS             9509..11686
                     /locus_tag="VBC94_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO6979310.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            11683..12564
                     /locus_tag="VBC94_001034"
     CDS             11683..12564
                     /locus_tag="VBC94_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEO6979311.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYTKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRT
                     AQQQYDVTQHIPLSEAQAGDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADL
                     TSPYWQQHLVGAGRIKQ"
     gene            12561..13493
                     /locus_tag="VBC94_001035"
     CDS             12561..13493
                     /locus_tag="VBC94_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6979312.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
     gene            13738..13854
                     /locus_tag="VBC94_001036"
     CDS             13738..13854
                     /locus_tag="VBC94_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO6979313.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            13870..15789
                     /gene="tet(M)"
                     /locus_tag="VBC94_001037"
     CDS             13870..15789
                     /gene="tet(M)"
                     /locus_tag="VBC94_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO6979314.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            15908..16075
                     /locus_tag="VBC94_001038"
     CDS             15908..16075
                     /locus_tag="VBC94_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO6979315.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(16135..16488)
                     /locus_tag="VBC94_001039"
     CDS             complement(16135..16488)
                     /locus_tag="VBC94_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6979316.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            16993..17415
                     /locus_tag="VBC94_001040"
     CDS             16993..17415
                     /locus_tag="VBC94_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6979317.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            17412..17642
                     /locus_tag="VBC94_001041"
     CDS             17412..17642
                     /locus_tag="VBC94_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6979318.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            18103..18306
                     /locus_tag="VBC94_001042"
     CDS             18103..18306
                     /locus_tag="VBC94_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO6979319.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            18388..19605
                     /locus_tag="VBC94_001043"
     CDS             18388..19605
                     /locus_tag="VBC94_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO6979320.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
BASE COUNT         6631 a         3438 c         4097 g         5868 t
ORIGIN      
        1 taaaggaaat aaactaccag caacaggtga gaatgcaact ccattcttta atgttgtagc
       61 tttgacaatt atgtcatcag ttggtttatt atctgtttct aagaaaaaag aggattaatc
      121 ttttgaccta aaatgtcact aaacttttca ccatttattg gtgtgaacac attaataaag
      181 ttatgtatct ctcttcaaca aacttaatta aagtgtttca atttttcgag tttaattctt
      241 gaaaaatcct atcgagatta ttaatttcga taggattttg attttgtgta agcgtccaat
      301 aacgaggtat cttccatttt cttccctatt ccgtaagcat ccaatatacc ttgttattgg
      361 acgcttacta taaaactgaa gaaatgatcc tatttagttg aaatttgtaa atgaagtgta
      421 tcattagagc ctaagatgtt aatcctaggc tctcttttga tgatttaaaa tttttaggtg
      481 taatcaaatg taaggttaga tgcaaaattt tctaaaatga ctgtttgtct tttcaagtaa
      541 ttaaatgtat ttaagggtaa tttataaaaa taaaaggagt gtgtcaataa tgttgtgtaa
      601 acacactggc aaagctggga ttgttaatca aataggcttt caagtgtgtc tgtacattgg
      661 ccaaaacctt tgtggattcg ttgttcttgc ttgaagttgt aatcattaaa tagtgtgact
      721 agatagtatt ctagagcctc ttcattggga aagacgattt ccttttttgt ttgcgttgta
      781 ttgcatttag tgatacaata gttatacaaa aatgatttga ggtgatatta tgtcaacagt
      841 agcagtaaga gttgatgatc aattaaaaga tgatgctaca gaactttttc aatccttagg
      901 gttggatatg agcacagcag taaaaatgtt tttgattcaa tctgttaaaa ctcaaagtat
      961 tccttttgaa ataaaaaata aatcatctgt ttctgatgaa gaatttcaaa atttggtaga
     1021 aacaaaatta aagggaatac gagttaaggc tagtgatcca gaaagtgtaa atgcgttttt
     1081 tggagatgaa gatttttcag aatatgagga atattttaaa tgagtgatat tgaggtatat
     1141 tctgtattag tttctcgtat tgaatattct gatggtacag gttcaaaggt tcgtcctgct
     1201 gtagtagtca aatttaatga tgaagtaata aaaactttaa gatttactac taaatatgaa
     1261 aataagtctg acaatataag aagtcagtat ttagaagtta ttgattaggc aaaagcaaat
     1321 ttaaataggc gttcttggat agatactatt caatattatg atgttgagaa taaagggttc
     1381 aatattaaaa ttataggtaa gttaagcgat agagatgttg agaggttaaa agattttctc
     1441 atagcaaagg aagtttagtt atgaaaaaga aattattagg attgatgatt ttagctatat
     1501 ctacagtatt tttagttgct tgttcgacta attcgttaaa tgtaaagtat tataaagtat
     1561 atgatggtga aaaaatgtta gttttagaaa ttaatgatga atcaggaatt tatcataatg
     1621 aaggaaactt tgtagtaact aatattgata ctaaaagtaa aagttttgta cttaaaggtg
     1681 ggtctgctaa gtatactgtt ttatatgatg ttaaaagtga tggtaaattg aatgttgatt
     1741 tagggaactt ttattataat gcttctggta aggagacata ttataaagaa ggtagcaagg
     1801 ctttagaaaa agcattaaga gataaagaat aaagttagga ctattttcct aacttatttt
     1861 taacaaaaaa atagcataaa aatctagtta tccgcataaa aactggactt atcacacttt
     1921 atcaaggtca aaaccactca atttactact aatttactac ttatgaatga gctttgatac
     1981 gacgatttat ccttgaaaag tgaagatata aagatacttc caataaaatt tgaatattta
     2041 ataggtagac acttcaaaaa atgaggtgtc tattttttta cccgattttg aaaggaagtg
     2101 aacttatgaa aacaaaaaat caagaatcaa aaggtcgttc cccactcttt aagaccatca
     2161 aacattcatt cagccaataa aaaagaaagg ataggtaaaa atatggaact taaatttgtg
     2221 attcccaaca tggaaaaaac attcggcaat ttagaatttg ctggcgagga taaagtcgtt
     2281 cagcgaagaa tcaacggacg gctaactgtc ttatcaagaa gctataatct ctattctgat
     2341 gttcaaagag cagatgatat tgtggtggtg cttcctgctg aagctggcga aaaacatttc
     2401 ggctttgagg aacgtgtgaa gttagtcaat ccacgtatta ccgcagaggg ctacaaaatc
     2461 ggcactcgtg gttttacaaa ttacctttta catgctgacg acatgataaa agaataaaga
     2521 aagagaggaa aaatgatgag attagcaaat ggcattgtat tagataaaga cacgactttt
     2581 ggagaattga aattctctgc tctacgtcgt gaagtgagaa tccaaaatga agacgggtcg
     2641 gtttcagatg aaatcaagga acgtacctat gacttaaaat ccaaaggaca aggacgcatg
     2701 attcaagtaa gtattcctgc cagcgtgcct ttgaaagagt ttgattataa cgcacgggtg
     2761 gaacttatca atcccattgc ggacaccgtt gctactgcca cctatcaagg agcagatgtt
     2821 gactggtata tcaaggcaga cgatattgtg ctgacaaagg attctagttc attcaaagct
     2881 caaccacaag caaagaaaga accgacacaa gacaaatagt cgctaggtag aaaggagact
     2941 ttttcgcatg aaacagcgtg gtaaaaggat tcgcccatct ggtaaagatt tagtctttca
     3001 ttttacgata gcgtcactcc tgcctgtttt cctgctggtt gtcggactgt ttcatgtgaa
     3061 gacaatccag cagatcaact ggcaggattt taacctatca caagcagata agattgacat
     3121 tccctattta attatcagtt tcagtgtcgc aattcttatc tgcttgctgg tagcgtttgt
     3181 attcaaacgg gttcgctatg atacggttaa acaactttac caccgtcaaa aactggcaaa
     3241 gatgatactt gaaaacaagt ggtatgaatc tgaacaggtc aaaacagagg gtttctttaa
     3301 agatagtgct ggtcgtacaa aggaaaagat aacctacttc cctaaaatgt attatcgact
     3361 taaaaatggc ttgatacaga tacgggtgga aatcacgctg ggaaaatatc aagaccaact
     3421 cttacacttg gaaaagaaat tagagagtgg cttgtactgt gagctgacgg ataaagagtt
     3481 aaaggattcc tatgtggaat atactttgct ctatgacacc atagccagtc gtatttctat
     3541 tgatgaagta gaagctaaag atggtaaact tcgcttaatg aaaaacgtat ggtgggaata
     3601 tgataagctc cctcatatgt tgattgctgg tggtacaggt ggcggtaaaa cttactttat
     3661 actgacactg attgaagcct tgcttcatac agattcaaaa ctgtatattc ttgacccgaa
     3721 aaatgctgat cttgcggact taggttctat gatggcaaat gtctactata gaaaagaaga
     3781 cttgctttct tgcattgaaa cattctatga agaaatgatg aaacgtagtg aggaaatgaa
     3841 gcagatgaag aactataaga ctggcaaaaa ttatgcttac ttaggtctcc cggcacactt
     3901 cttaatcttt gatgaatacg tcgctttcat ggaaatgctg ggaacaaaag aaaacaccgc
     3961 agttatgaat aagctgaaac agattgtcat gttaggtcgt caagctggct tctttctaat
     4021 actggcttgt caacgtccag acgcaaaata tttaggcgac ggaatccgtg atcagtttaa
     4081 tttcagagtg gctttaggtc gtatgtctga aatgggctat ggcatgatgt ttggcagtga
     4141 cgtacaaaag gatttcttct taaagcgaat caaaggtcgt ggctatgttg atgtaggaac
     4201 aagtgtcata tcagagtttt atactcccct tgtaccaaaa ggatatgatt tcttggagga
     4261 aattaaaaag ttatccaaca gcagacagtc cacgcaggcg acgtgcgaag cggaagtcgc
     4321 aggtgtggac tgatcttgct ggctggtgtg gcaatagcca cgccagcact taaccccccg
     4381 tatctaacag gggggtacaa atcgacagga aacagtcaaa aaaacattag aaaatccttt
     4441 ggttacaagg gatttacaaa atttcagcgt atgtcaaatg ggctttaaaa gttgacatac
     4501 gcctttttga ttggagggat ttttactgaa tgaacaaact tggttacagc atttaaaaga
     4561 aaaacgcttg gcttatggac tatctcaaaa ccgtttagct gttgcgactg gtattacaag
     4621 gcagtatcta agcgatattg aaacaggaaa agtcaagcca tcagaggatt tacagcagtc
     4681 cctttgggaa gctctggaac gcttcaatcc cgacgctccc cttgaaatgc tgtttgatta
     4741 tgtaagaatt cgctttccga caacagacgt acagcaggtg gtcgaaaaca tcttacaact
     4801 gaaactgtcc tattttcttc atgaggacta tggtttctat tcttattcag agcattatgc
     4861 tttaggcgac atattcgtcc tttgctccca tgaactggac aaaggagttc tggtggaatt
     4921 gaaaggtcgt gggtgcagac aatttgaaag ctatcttctg gcacaacaaa gaagctggta
     4981 tgagttcttt atggacgttt tggtggctgg cggtgtgatg aaacgccttg accttgccat
     5041 taacgataag acagggattt taaatatccc tgtactcact gaaaagtgcc aacaggaaga
     5101 atgtatctcc gtcttccgca gttttaaaag ctatcgcagt ggcgaactgg tacgcaaaga
     5161 ggaaaaggaa tgtatgggaa acaccctcta tatcggttca ttacaaagtg aagtttattt
     5221 ctgtatctat gaaaaggact acgagcagta caagaaaaat gatattccca ttgaagacgc
     5281 agaagtaaaa aaccgttttg agattcgatt gaaaaatgag cgtgcctatt atgcagtccg
     5341 tgatttactc gtctatgaca atccagagca taccgccttt aaaattatca atcggtatat
     5401 ccgttttgta gataaagacg attccaaacc tcgttctgat tggaaactga atgaagaatg
     5461 ggcttggttt attgggaaca atcgtgaacg attaaaacta accacaaaac cagagcctta
     5521 ctccttccaa aggacgctga actggctatc tcatcaagtt gccccgacct taaaggttgc
     5581 gattaaactt gatgaaatca accagacgca ggttgtaaaa gacattctcg accatgcgaa
     5641 actgacagac cgacacaagc agattttgaa gcaacagtca gtaaaagaac aggacgtgat
     5701 aacaacaaaa aaataactca aatacaaatt cattgaatat agagaggaga acatttttat
     5761 gaattttgga caaaaccttt ataactggtt tctatcaaac gctcaatcac tggtgctttt
     5821 agcaatcgtt gtgattggct tgtatcttgg cttcaagcgt gagtttagca aactgattgg
     5881 ctttttaatt attgcgatta ttgcggttgg cttagtcttc aacgctgctg gagtaaaaga
     5941 cattttacta gagctattca atcgcattat tggtgcttaa ataaaaccgt tcttttgtgg
     6001 aatataagtg gttttcttat gttccgcaaa ggaatggtac accaaacgaa gtgcggtagg
     6061 gatttttgaa tctctacaaa gaaaggacgt gaatatatgg acgatatgca agtctatatt
     6121 gcgaatttag gcaaatacaa tgagggcgaa ttggtcggtg cgtggtttac ctttcccatt
     6181 gactttgagg aagtcaaaga gaaaatcggc ttgaatgatg aatatgagga atacgccatt
     6241 catgactacg agttaccctt tacggttgac gaatacactt ccattggcga actcaatcga
     6301 ctatgggaaa tggtatcgga attacccgaa gaattacaat cggagctatc tgctctgctc
     6361 actcattttt caagcattga agaactaagc gaacatcaag aggatattat cattcattcc
     6421 gattgtgatg atatgtatga cgtggcacgc tactacattg aagaaacggg tgctttaggc
     6481 gaagtaccag ctagtcttca aaactatatt gattatcaag cctatggtcg ggatttagac
     6541 ctttcaggaa cgtttatctc aaccaatcat gggatttttg aaatcgtcta ttaaatctgt
     6601 cggtacatta ctactggcag attttctatt ttacggggtg gctcaatcag ctacccctat
     6661 tttttatgaa aggattgatt acatgaagaa aatacgaagc tataccagta tctggtctgt
     6721 ggaaaaggta ctgtattcta tcaatgattt tagacttccg tttcccataa cctttacgca
     6781 aatgacatgg tttgtcgtgt cactctttgc agtgatgata cttggcaact tgccccctct
     6841 ttccatgata gagggagcat ttctcaaata ctttgggatt cctgtggctt tcacatggtt
     6901 tatgtctaca aaaacttttg atggtaaaaa gccttatgga tttttgaagt ctgtcattgc
     6961 ttatgcactg cgaccaaagc tgacctatgc aggaaaaaaa gtaacgcttg gcagaaacca
     7021 gccacaagaa gccattacag cagttaggag tgaattttat ggcatatcca attaaataca
     7081 ttgaaaacaa tctcgtctgg aataaagacg gggaatgtta tgcttactat gagcttgttc
     7141 cttacaatta ctcatttcta agtccagaac agaaaataca agtgcatgat tctttcagac
     7201 agcttatcgc acaaaatcgt gatggcaaaa ttcatgcttt acaaatcagt acagaatcca
     7261 gcatacgttc tgcacaagag cgttccaaaa atgaagtcac tggcaagctc aaagcggttg
     7321 cctatgacaa aatcgaccaa cagacagacg ctttaatatc catgattggc gaaaatcaag
     7381 tgaactaccg tttctttatc ggctttaagt tgcttctcaa cgatcaggag ttttctatga
     7441 aaagtcttac cgttgaagca aaaaatgctt tgtctgattt tgtctatgat gtgaaccata
     7501 agctgatggg cgattttgtt agtatgagta atgatgaaat cctgcgtttt cagaagatgg
     7561 aaaagctctt agaaaataaa atctctcgtc gtttcaaaat ccgcaggtta gataaggacg
     7621 acttcggcta tctgattgaa cacctttacg gacagacagg cactgcctat gaagagtatg
     7681 agtaccatct atcaaagaaa aagctggata atgaaacgct gattaaatac tatgacttga
     7741 ttaagcctac tcgctgtttg gtggaagaaa aacagcgata tttgaaaatc cagcaggaag
     7801 atgaaaccgt ctatgtagct tactttacca ttaacagcat tgtcggagaa ctggacttcc
     7861 cgtcctctga aatcttctac taccagcaac agcaatttac attcccgatt gatacgtcaa
     7921 tgaatgtgga aattgtagcg aatcgtaaag ccctatctac tgtccgcaat aaaaagaaag
     7981 aactgaaaga cttggataac cacgcttggc aaagtgataa tgaaaccagc tccaatgtgg
     8041 cggaagctct ggaaagtgtg aatgagctgg aaaccaattt agaccaaagc aaggaatcta
     8101 tgtacaagct gtcttatgtg gtaagggtat cagcaaatga tcttgacgaa ctcaaacgtc
     8161 gttgtaatga agtgaaagat ttttatgacg atttaagcgt aaaactggta cgaccatttg
     8221 gggatatgct cggcttacat gaagaatttt tacctgccag caagcgttat atgaatgatt
     8281 atattcaata cgtgacctct gatttcctcg ctggtttagg ttttggtgct actcaaatgc
     8341 tgggggaaaa tgaggggatt tatgttggct acagcttaga tactggacgc aatgtctatc
     8401 tgaaacctgc tcttgccagt caaggggtta agggttcagt aaccaatgcg ttagcgtcgg
     8461 cttttgttgg ttcgctgggt ggtggtaaat cctttgcgaa taaccttatc gtctattatg
     8521 cggtgcttta tggggcacaa gcagtgattg tagacccaaa agcagaacgt ggcagatgga
     8581 aagaaacctt gccagagatt tcccatgaaa tcaatatcgt cactctgact tctgatgaga
     8641 aaaacaaagg cttacttgac ccttatgtga ttatgaaaaa tcccaaagat tctgaatcac
     8701 tggctattga tattctgaca ttccttacgg ggatttcctc tcgtgatggg gaacgcttcc
     8761 caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtacgaggg ttgatgaaag
     8821 tgattgagga attacgggtt gagaatacgc cactaagtac cagtatagcc gaccatatcg
     8881 aaagttttac agactatgac tttgcacatt tattattcag taatggttat gtggagcagt
     8941 ctatcagctt agaaaaacaa ctgaacatta tacaggttgc ggacttggta cttcccgaca
     9001 aggaaacttc ctttgaggaa tataccacta tggagctttt atccgttgct atgctgattg
     9061 tcattagtac ctttgcttta gactttatcc atacagaccg aagcattttc aagattgtag
     9121 atttagacga agcatggagc tttttacagg tagcacaagg aaaaacacta tctatgaagc
     9181 tggttcgggc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa aatacagacg
     9241 acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgcattt cgttccactg
     9301 accttaacga gattaaaaag accttagcct tttttggtgt agacccagag gacgaaaaca
     9361 atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat ttatatggtc
     9421 gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc tttgatacca
     9481 gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atagtaaaca gaataaaatc
     9541 aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca cacttgtgat
     9601 tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct tggtagatga
     9661 tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc aactggattt
     9721 ttatgtggat aatagctggg gctggcttcc gtggaactgg tcggacggga ttggaaaaca
     9781 ggtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtt tgtatgtttc
     9841 caatgcgaca ggttacttag tacaggaagc ctattcctta gacttcattt ccgctacagc
     9901 agattccatt ggtaagaata tgcagacctt agctggtgtg agtgcaaacg gattttcaac
     9961 agagggtttc tatgttggat tcctcttact cttgattttg gttcttgggg tttatgttgc
    10021 ctatacggga ctgataaaga gagaaaccac aaaggcaatt catgccatta tgaattttgt
    10081 gctggtgttt atcctatcgg cttcctttat tgcctacgct cccgactaca ttaaaaaaat
    10141 caatgacttt tcatcagaca tcagtaatgc cagtttatca cttggcacga agattgtcat
    10201 gccccattcc gatagtcaag gcaaggacag cgtggactta atcagagata gcctgttttc
    10261 catacaggtt cagcaaccgt ggctactgct tcaatacaac agttcagaca ttgaaagtat
    10321 cggtattgac cgtgtggaaa gcctgctctc caccagccca gattccaaca atggcgaaga
    10381 cagagaaaaa attgttgcgg aagaaattga agacagaagc aataccaatc taaccattac
    10441 aaagaccatt aaccgtttag gtacagtctt cttcctattt gtcttcaata ttgggatttc
    10501 catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt ttatcatcta
    10561 tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg atggtatgtc
    10621 aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa tcacattgat
    10681 tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg gttatccgtt
    10741 ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttca agctgggcga
    10801 tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa gtcgtgtgat
    10861 gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga aacttggacg
    10921 ttccatgact actctagggg ctgggtctgc cattgttaca ggtaaaaaag gacagtcggg
    10981 ttcggggagt tctgcaagga cacaagcaga tcactcccga ccagacggaa aggaaaaatc
    11041 aacacttgga aaacgtatcg gtcaaaccat cggtacagta gctgatacca aagacagaat
    11101 ggtagacact gctagtggtt tgaaagaaca ggttaaagat ttgccgacca atgcaagata
    11161 tgcagtatat caaggaaaat ccaaagtaaa agagaatgtc cgtgatttaa ccagtagtat
    11221 ttctcaaacc aaagcggaca gagccagtgg acgcaaggaa cagcaggaac aaaggcgaaa
    11281 aaccattgcg aagcgtcgct ctgaaatgga acaggtcaaa cagaaaaaac agcctgcttc
    11341 ttctgttcat gaaagaccga ctacaagaca agaacaatat catgatgaac agacctcaaa
    11401 acagtctaat attcagactt catataagga atctcaacaa gccaaacaag agcgtccagc
    11461 agttaagtcc gatttttcaa gtccaaaagt ggaacgccaa ggcaataccg ttcaagaaaa
    11521 aaccgttcaa aagccagcaa cttcaaccac tacagcagat agaacttcac aacgtccaat
    11581 cacaaaagaa cgtccgtcta ctgttcaaag agtaccacta caaaatacaa gaagtagacc
    11641 accaatcaaa accgccacca ttaagaaagt cggtaagaaa ccatgaagtt gaaaacttta
    11701 gtgattggtg gttctggatt attcttgatg gtcttctcac tgcttctgtt tgttgccatt
    11761 ttattttcag atgaacagga cagcggaatt tccaatattc attatggagg tgtgaatgtt
    11821 tccgcagaag tgctggctca taagcctatg gtagaaaaat ataccaaaga atatggcgtt
    11881 gaagaatatg tcaacatact tcttgcgatt atacaggtgg aatcgggcgg tactgcggaa
    11941 gatgttatgc agtcctcgga atccctcggt cttccaccta attcattgag tacagaagaa
    12001 tccattaagc aaggtgtgaa gtatttcagt gaattattag ccagtagcga aaggctcagt
    12061 gtagatttag aatcggttat ccagtcctac aattatggtg gtggtttctt agggtatgtg
    12121 gctaatcgtg gaaataaata tacctttgaa ctggctcaaa gtttctcaaa agagtattca
    12181 ggtggcgaaa aagtgtctta ccccaatccc atagccatac ctatcaatgg gggctggcga
    12241 tacgtttacg gtggagcttc cccgactact tcttttgatt gtagcggact gacacaatgg
    12301 acgtatggaa aagctggaat taacttacca cgaaccgcac aacagcaata tgatgtgacc
    12361 cagcatatcc cactatcgga agcacaagct ggcgatttgg ttttctttca ttctacctat
    12421 aacgctggct cttatattac tcatgttggg atataccttg gcaataaccg tatgtttcat
    12481 gcaggcgacc caatcggtta tgccgactta acaagcccct actggcaaca gcatttagtg
    12541 ggagcaggac gaatcaaaca atgagaaagg aagatttaat gatgaaattt agaaaaaatc
    12601 agaataaaga aaaacagata ccaaaggaaa agaaacctcg tgtctataag gtcaatcctc
    12661 ataaaaaggt tgtgattgcc ttgtgggtac ttttagggct tagtttcagc tttgcgatat
    12721 tcaagcactt tacagctata gatactcata ctattcacga aacaactatc atagaaaagg
    12781 aatacgttga tactcatcat gtagaaaatt ttgtagagaa ctttgcgaaa gtctactatt
    12841 catgggagca atccgataag tccattgata atcgaatgga aagtctaaaa ggctatctga
    12901 cagatgaact tcaagctctc aatgttgata cagtacgcaa agatattcct gtatcgtctt
    12961 ctgtaagagg atttcagata tggacggtag agccaactgg cgacaatgag tttaatgtaa
    13021 cctacagtgt agaccagctc attacagagg gagaaaatac aaagaccgtc cactctgctt
    13081 atatagtgag tgtctatgta gatggttctg gaaatatggt actggttaag aatccgacca
    13141 ttaccaacat acctaagaaa tcaagttata aaccaaaagc cattgaaagt gaggggacgg
    13201 ttgattccat tacaaccaat gaaatcaatg agtttttaac gacgttcttc aagctctatc
    13261 ctacagcgac agccagtgaa ctttcctact atgtgaatga cgggatatta aaaccaatcg
    13321 gaaaagagta catctttcaa gaactggtaa atcctattca caatcgtaag gataatcaag
    13381 tcacggtatc gctgacagtg gagtatatcg accagcagac caaagcaacg caggtatctc
    13441 aatttgattt ggtacttgaa aagaacggga gtaattggaa gattgtaaaa taacaaatat
    13501 tggtacatga ttacagatac tttgtaatca tgtactcttt ttgataaaaa attggagatt
    13561 cctttacaaa tatgctctta cgtgctatta tttaagtgac tatttaaaag gagttaataa
    13621 atatgcggca aggtattctt aaataaactg tcaatttgat agcgggaaca aataattaga
    13681 tgtccttttt taggagggct tagttttttg tacccagttt aagaatacct ttatcatgtg
    13741 attctaaagt atccagagaa tatctgtatg ctttgtatac ctatggttat gcataaaaat
    13801 cccagtgata aaagtattta tcactgggat ttttatgccc ttttgggttt ttgaatggag
    13861 gaaaatcaca tgaaaattat taatattgga gttttagctc atgttgatgc aggaaaaact
    13921 accttaacag aaagcttatt atataacagt ggagcgatta cagaattagg aagcgtggac
    13981 aaaggtacaa cgaggacgga taatacgctt ttagaacgtc agagaggaat tacaattcag
    14041 acaggaataa cctcttttca gtgggaaaat acgaaggtga acatcataga cacgccagga
    14101 catatggatt tcttagcaga agtatatcgt tcattatcag ttttagatgg ggcaattcta
    14161 ctgatttctg caaaagatgg cgtacaagca caaactcgta tattatttca tgcacttagg
    14221 aaaatgggga ttcccacaat cttttttatc aataagattg accaaaatgg aattgattta
    14281 tcaacggttt atcaggatat taaagagaaa ctttctgccg aaattgtaat caaacagaag
    14341 gtagaactgt atcctaatat gtgtgtgacg aactttaccg aatctgaaca atgggatacg
    14401 gtaatagagg gaaacgatga ccttttagag aaatatatgt ccggtaaatc attagaagca
    14461 ttggaactcg aacaagagga aagcataaga tttcataatt gttccctgtt ccctgtttat
    14521 cacggaagtg caaaaaacaa tatagggatt gataacctta tagaagtgat tacgaataaa
    14581 ttttattcat caacacatcg aggtccgtct gaactttgcg gaaatgtttt caaaattgaa
    14641 tatacaaaaa aaagacaacg tcttgcatat atacgccttt atagtggagt actacattta
    14701 cgagattcgg ttagagtatc agaaaaagaa aaaataaaag ttacagaaat gtatacttca
    14761 ataaatggtg aattatgtaa gattgataga gcttattctg gagaaattgt tattttgcaa
    14821 aatgagtttt tgaagttaaa tagtgttctt ggagatacaa aactattgcc acagagaaaa
    14881 aagattgaaa atccgcaccc tctactacaa acaactgttg aaccgagtaa acctgaacag
    14941 agagaaatgt tgcttgatgc ccttttggaa atctcagata gtgatccgct tctacgatat
    15001 tacgtggatt ctacgacaca tgaaattata ctttctttct tagggaaagt acaaatggaa
    15061 gtgattagtg cactgttgca agaaaagtat catgtggaga tagaactaaa agagcctaca
    15121 gtcatttata tggagagacc gttaaaaaat gcagaatata ccattcacat cgaagtgccg
    15181 ccaaatcctt tctgggcttc cattggttta tctgtatcac cgcttccgtt gggaagtgga
    15241 atgcagtatg agagctcggt ttctcttgga tacttaaatc aatcatttca aaatgcagtt
    15301 atggaaggga tacgctatgg ttgcgaacaa ggattatatg gttggaatgt gacggattgt
    15361 aaaatctgtt ttaagtatgg cttatactat agccctgtta gtaccccagc agattttcgg
    15421 atgcttgctc ctattgtatt ggaacaagtc ttaaaaaaag ctggaacaga attgttagag
    15481 ccatatctta gttttaaaat ttatgcgcca caggaatatc tttcacgagc atacaacgat
    15541 gctcctaaat attgtgcgaa catcgtagac actcaattga aaaataatga ggtcattctt
    15601 agtggagaaa tccctgctcg gtgtattcaa gaatatcgta gtgatttaac tttctttaca
    15661 aatggacgta gtgtttgttt aacagagtta aaagggtacc atgttactac cggtgaacct
    15721 gtttgccagc cccgtcgtcc aaatagtcgg atagataaag tacgatatat gttcaataaa
    15781 ataacttagt gtattttatg ttgttatata aatatggttt cttgttaaat aagatgaaat
    15841 attttttaat aaagatttga attaaagtgt aaaggaggag atagttatta taaactacaa
    15901 gtggatattg tgtcctgtat gtggaaataa aacacgatta aagataaggg aagatactga
    15961 attaaaaaaa ttccccctct attgtccgaa atgcagacaa gaaaatttaa ttgaaataaa
    16021 gcagttcaaa gtaactgtga ttacagagcc agacgcaaag acgcagagcc gataaaatga
    16081 gattaataca atctcatttt atcggctctt tccgttatgt atggattctt ttaattagtc
    16141 ttcgatgttt cttgcttcgt tgataccgct ggctaaagat tccattaagg atagttcttt
    16201 gtctgtaaag ctatccatgt atttctctat ctgtaatcgt cgggtgcttt ttaccaagtt
    16261 attagcaggt aagaaaaatt catcaacgga aacatgaagt aacgatacaa ggtcataaag
    16321 aacttgtatg ctggggtgtt gccctttatt ttcaatatta gttaagtacc gtgggtcaat
    16381 ttcaatcaat gctcccactt gttcacgagt taaacctcgt ttcaatcgag cttctttaat
    16441 ggctaaacca aaggctctaa aatcatattt atcttctttt ttacgcatag tagaccacct
    16501 ctatacattt tattattcct actgaattaa aaacaggtat agaaaaacgt gttatatggt
    16561 ttataggttt atatttaata aaaagcacta ctaaacgcca ataaaaaaaa ccgttatatg
    16621 gtagtgctat ttacgctgtt aaaatattgt atattacttc caaatggcgg tttgttggag
    16681 gtcaacgtcg ccatgaagta catcatatac aataaatttc cttacattgg gttcttgtca
    16741 aaaaaagtcg tctatctgca atagataagt acgtccacca atgtggtttt ataaatcata
    16801 tagatagaat aacagaagca tgtaaacaga gaaataaatc tgtttatatg cttttttggc
    16861 tattcagaac ttttttacaa agtttattta tcagtaatgc aacaaatccc cctttcacat
    16921 tgggactaag agtgaaagga gataaacgag caaggctcac ttcctttcct agacagaaag
    16981 ggggtgagaa acatgaaacc atcttctttt cagaccacaa tagaaaatca gtttgactat
    17041 atctgtaaac gtgctatgga agacgagcga aagaattata tgctttatct ttcaaggatt
    17101 gcaaagcgtg aggtgtcctt ttcggatgtt ggcgattatc ttgttagcca gtttgcgaca
    17161 acagataact attcaactga ctttcagatt tttacactca atgggttatc agtaggcgtt
    17221 gaaaatgatt tgttgagtga agcattacgt gagttgccag acaagaaacg tgaaattcta
    17281 ctgctgtttt actttatgga catgagcgat tcagaaattg cagacctgtt gaaattgaac
    17341 cgttctactg tctatcggca tagaaccagt ggactagcct taattaaaaa gtttatggag
    17401 gaatttgaag aatgaaaaca caatatccta tgattccctt tcctctcatt gtaaaggcaa
    17461 cagatggcga taccgaagcg attaaccaga ttctacatca ttacagaggg tacataacga
    17521 agcgttccct acgacttatg aaagatgaat atggcaatca aagtatggtc gttgatgaag
    17581 tcttacgtgg aagaatggaa accagactga ttacaaagat tttgtcattt gaaattaagt
    17641 aatatcctct ctcctttcgt ggaagcgtgc taaaccattc cacgcttccc gaacagggag
    17701 gtttgttatt ccaccaaagc atattgagct ttcaatgtgt tttgataggc taacgagcca
    17761 ttgttctttg aaaactgaat aaaagtaatc gaatacgttt cgataagaaa agagccaacg
    17821 gaactaaccg ccatgaccta tcttataaag atagcgagcg attcatgtta gtgatccgag
    17881 aagcaatctt tagcaggatt gcctgcaacg acattcttat cgtgataatg atactcccat
    17941 acagtcaata gtccgagcgt gataaaaccg tcgcaggcaa tgagtatggc tacatgagaa
    18001 ccatgcaggg gtggaactcc cgtgagcttt gctaaagctg ttcgattgct ggtaaaacaa
    18061 cttttatgaa atccaaataa gtgatttgga aaggaggatt ttatgaagca gactgacatt
    18121 cctatttggg aacgttatac cctaaccatt gaagaagcgt caaaatattt tcgtattggc
    18181 gaaaacaagc tacgacgctt ggcagaggaa aataaaaatg caaattggct gattatgaat
    18241 ggcaatcgta ttcagattaa acgaaaacaa tttgaaaaaa ttatagatac attggacgca
    18301 atctagcgtc gccaaagggt cttgtatatg ataaaatagt attaagtcgt atcaaggctc
    18361 tttccataaa ggaaaggagc aaatgccatg tcagaaaaaa gacgtgacaa taaaggtcga
    18421 atcttaaaga ctggagagag ccaacgaaaa gacggaagat acttatacaa atatatagat
    18481 tcatttggag aaccgcaatt tgtttactcg tggaaacttg tggctacaga ccgagtacca
    18541 gcaggaaagc gtgattgtat ctcacttaga gagaaaatcg cagagttaca gaaagacatt
    18601 catgatggta ttgatgttgt aggaaagaaa atgacactct gccagcttta cgcaaaacag
    18661 aacgctcaaa gaccaaaggt tagaaaaaac actgaaactg gacgcaaata tcttatggat
    18721 attttgaaga aagacaagtt aggtgtaaga agtattgaca gtattaagcc atcagacgct
    18781 aaagaatggg ctattagaat gagtgaaaat ggttatgctt atcaaaccat caataactac
    18841 aaacgttctt taaaggcttc attctatatt gctatacaag atgattgtgt tcggaagaat
    18901 ccatttgact ttcaactgaa agcagttctt gatgatgata ctgtccctaa gaccgtacta
    18961 acagaagaac aggaagaaaa actgttagcc tttgcaaaag ctgataaaac ctacagcaaa
    19021 aattatgatg aaattctgat actcttaaaa acaggtcttc gtatttcaga gtttggtggt
    19081 ttgacacttc cagatttaga ttttgagaat cgtcttgtca atatagacca tcagctattg
    19141 agagatactg aaattgggta ctacattgaa acaccaaaga ccaaaagtgg cgaacgtcaa
    19201 gttcctatgg ttgaagaagc ctatcaagca tttaagcgag tgttagcgaa tcgaaagaat
    19261 gataagcgtg ttgagattga tggatatagt gatttcctct ttcttaatag aaagaactat
    19321 ccaaaagtgg caagtgatta caacggcatg atgaaaggtc ttgttaagaa atacaataag
    19381 tataacgagg ataaattgcc acacatcact ccacatagtt tgcgacatac attctgtacc
    19441 aactatgcaa atgcaggaat gaatccaaag gcattacagt acattatggg acatgctaat
    19501 atagccatga cgctgaacta ttacgcacat gcaacattcg attctgcaat ggcagaaatg
    19561 aaacgcttga ataaagagaa gcaacaggag cgtcttgttg cttagtagta caaatgaatt
    19621 tactacttat ttaccacttc tgacagctaa gacatgagga aatatgcaaa gaaacgtgaa
    19681 gtatcttcct acagtaaaaa tactcgaaag cacatagaat aaggctttac gagcatttaa
    19741 gaaaatataa aaagataatt agaaatttat actttgtttt tatttaaaaa atgaacttca
    19801 aaatgcaaag tcttaatttt acttagcacc taatttttag ttaaataatt tgtatcccag
    19861 caataaaaag tatttttacc gaaatcaaag taagaaccaa aaagaagatg gggttactct
    19921 gtcttagttt ttcttggaaa gtgttatctg ttaattcaat gaaatcaagc gaggaaatga
    19981 gatagctttt atttaattga tgagttgctt tttccagatt atttggagtt attt
//
LOCUS       DATROQ010000033        19510 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.033, whole genome shotgun sequence.
ACCESSION   DATROQ010000033 DATROQ010000000
VERSION     DATROQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19510
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.033"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            144..1226
                     /gene="hrcA"
                     /locus_tag="VBC94_001044"
     CDS             144..1226
                     /gene="hrcA"
                     /locus_tag="VBC94_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEO6979321.1"
                     /translation="MCKSANFRKTERGDVFVITQRQNDILNLIVELFTQTHEPVGSKA
                     LQRTIDSSSATIRNDMAKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQD
                     IYHVIKAFDFEAFKLEDMLQKASHILSEMTGYTSVILDVEPARQRLTGFDVVQLSNHD
                     ALAVMTLDESKPVTVQFAIPRNFLTRDLIAFKAIVEERLLDGSVMDIHYKLRTEIPQI
                     VQKYFVTTDNVLQLFDYVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLR
                     QGLKEGEMASVQVADSQEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLV
                     NIIGKVLAAKLGDYYRYLNSNHYEVH"
     gene            1229..1801
                     /gene="grpE"
                     /locus_tag="VBC94_001045"
     CDS             1229..1801
                     /gene="grpE"
                     /locus_tag="VBC94_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEO6979322.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEEIVEEVIEEIP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            1982..3811
                     /gene="dnaK"
                     /locus_tag="VBC94_001046"
     CDS             1982..3811
                     /gene="dnaK"
                     /locus_tag="VBC94_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEO6979323.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVEEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIQSNSGLTDEEIDKMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKKAQESGNLDDMKAKLEALNEKAQ
                     ALAVKLYEQAAAAQQAAQGAEGAQSADSSSKGDDVVDGEFTEK"
     gene            4100..5215
                     /gene="dnaJ"
                     /locus_tag="VBC94_001047"
     CDS             4100..5215
                     /gene="dnaJ"
                     /locus_tag="VBC94_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEO6979324.1"
                     /translation="MNNTEFYDRLGVSKDASQDEIKKAYRRMSKKYHPDINKEAGAEE
                     KYKEVQEAYETLSDTQKRAAYDQYGAAGANGGFGGFDGGGFGGFEDIFSSFFGGGGMR
                     NPNAPRQGDDLQYRVNLSFEEAIFGAEKEVSYNRESSCHTCSGSGAKPGTSPVTCQKC
                     HGSGVINVDTQTPLGTMRRQVTCDVCQGSGQEIKEKCPTCHGTGHEKKTHKVSVKIPA
                     GVETGQQIRLTGQGEAGFNGGPYGDLFVIINVLPSQQFERNGSTIYYTLNIGFVQAAL
                     GDTIDIPTVHGAVEMSIPAGTQTGKTFRLRGKGAPKLRGGGQGDQHVTVNIVTPTKLN
                     DAQKEALHAFAEASGDKMVHPKKKGFFDKVKDALDVD"
     gene            5333..6580
                     /locus_tag="VBC94_001048"
     CDS             5333..6580
                     /locus_tag="VBC94_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEO6979325.1"
                     /translation="MVTKVEEIRSYLIASIQNGKLAPGDRLPSIRQLANQFSCNKDTV
                     QRVLMELRFDNYIYAKPRSGYYVFDSHQEEVEEGVSLPNSEIANIAYDDFRLCLNETL
                     IGREDYLFNYYYRQEGLLDLSKAVAKLMEETGVYVPLDDIVITAGTQQALFILTQVTF
                     PNRKSRVLIEEPTYPRMIELIKTQNLPYETIPRGTHGIDFQRLEEIFQTQSIKFFYVI
                     PRMHNPLGTSYNPVEMKRLIEMAEKYDVYIVEDDYMSDFASQSPLHYYDTHGRVIYLK
                     SFSKAIFPALRLAAICLPQALKSTFMAYKKLMDYDTNLILQKALALYIENGLYAKNSQ
                     YLKYRYQKDLANSKSILADHPNLPPYSLHHDSVLFDCSKLDNFKILRQYGDTLENYFC
                     QKSHQSLLQVKNDSCLKQFLGSL"
     gene            6651..7427
                     /gene="truA"
                     /locus_tag="VBC94_001049"
     CDS             6651..7427
                     /gene="truA"
                     /locus_tag="VBC94_001049"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEO6979326.1"
                     /translation="MTRYKAQISYDGSAFSGFQRQPNCRTVQEEIERTLKRLNSGNDV
                     IIYGAGRTDAGVHAYGQVIHFDLPQARDVEKLRFGLDTQCPDDIDIVKVEQVSDDFHC
                     RYDKHIKTYEFLVDIGRPKNPMMRNYATHYPYPVIIELMQEAIKDLVGTHDFTGFTAS
                     GTSVENKVRTIFDAKIQFEASKNLLIFTFTGNGFLYKQVRNMVGTLLKIGNGRMPISQ
                     IKTILQAKNRDLAGPTAAGNGLYLKEIIYEDEECFSNFRK"
     gene            7390..8148
                     /locus_tag="VBC94_001050"
     CDS             7390..8148
                     /locus_tag="VBC94_001050"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO6979327.1"
                     /translation="MKTRNVLAISGNDIFSGGGLHADLATYVVNKLHGFVAVTCLTAM
                     SDKGFEVIPIEASILKQQLESLKDVEFGSIKLGLLPNVETAQVVLEFVKSKQECPVVL
                     DPVLVCKENHDLEVSQLREQLIAFFPYADVITPNLVETQLLTGLSIENLDQMKIAAEK
                     LYDMGAKHVVIKGGNRLNAEEATDLYYDGERFETYVFPVVDANNTGAGCTFASSIASQ
                     LAMGKNVEDAVKMSKGFVYQAIKASDKYGVVQHF"
     gene            8158..8622
                     /locus_tag="VBC94_001051"
     CDS             8158..8622
                     /locus_tag="VBC94_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6979328.1"
                     /translation="MRKEKTSQLVQLAIVTAISIVLGMFISIPTPTGFLTLLDAGIFF
                     AAFYFGKKEGAVVGALAGFLIDLLKGYPNWMFFSLLIHGAQGYLAGLPGRRRLLGLIS
                     ATLVMVLGYAIASGLMYGWGAVLPDIPGNIMQNMVGMVVGFALNKSLERVKK"
     gene            8619..9188
                     /locus_tag="VBC94_001052"
     CDS             8619..9188
                     /locus_tag="VBC94_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEO6979329.1"
                     /translation="MILDYNKLKQETKAIVVDIIERSALKKGQIFVLGLSSSEVSGGL
                     IGKNSSSEIGEIIVEVILKELHSRGIYLAVQGCEHVNRALVVEAELAERQQLEVVNVV
                     PNLHAGGSGQVAAFKLMTSPVEVEEIVAHAGIDIGDTSIGMHIKRVQVPLIPISRELG
                     GAHVTALASRPKLIGGARAGYTSDPIRKF"
     gene            complement(9225..10067)
                     /locus_tag="VBC94_001053"
     CDS             complement(9225..10067)
                     /locus_tag="VBC94_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEO6979330.1"
                     /translation="MITLEKFIDHLNVEEVLFTFFTKLISILLLIIAFVIVRQVINYL
                     FEKTVNRSLAFSRQKVARQKTLAKLSHNVLNYTLYFFLFYWILSILGVPISSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILLENQFDVGDIINVGAVSGTVTNVGIRTTQIHDFD
                     GTLHFIPNRNITIVSNKSRSNMRAQIDIPLFVHTNLDQISDIVTKINEEYVSKHPAIV
                     GEPTVFGPTTNANGQFVYRINIFTQNGAQFDIYAEFYKLYQKAILEEGIDLPTYNFSN
                     NHSR"
     gene            10224..11507
                     /locus_tag="VBC94_001054"
     CDS             10224..11507
                     /locus_tag="VBC94_001054"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEO6979331.1"
                     /translation="MSTSFENKATNRGIITFTISQDEIKPALDQAFNKVKKDLNVPGF
                     RKGHMPRTVFNQKFGEEALYENALNLVLPKAYEAAVAELGLDVVAQPKIDVVSMEKGQ
                     DWKLTAEVVTKPEVKLGDYKDLSVEVDASKEVSDEEVDAKVERERNNLAELTVKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEEQLVGSKAGQTVDVNVT
                     FPEDYQAEDLAGKDAKFVTTIHEVKTKEVPALDDELAKDIDDEVETLDELKAKYRKEL
                     ESAKEIAFDDAVEGVAIELAVANAEIVELPEEMVHDEVHRAMNEFMGNMQRQGISPEM
                     YFQLTGTTEEDLHKQYQADADKRVKTNLVIEAIAAAEGFEATDEEIEKEITDLASEYN
                     MEADQVRGLLSADMLKHDIAMKKAVDVITSSATVK"
     gene            11696..12280
                     /locus_tag="VBC94_001055"
     CDS             11696..12280
                     /locus_tag="VBC94_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEO6979332.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILEQRGRDNEMYFSDLVNDIQT
                     YLGKSDSAIRESLPFFYSDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     APKRKKKRVNAFMDGDEDAIDYNDDDPEDEDFTEETPSLEYDEENPDDEKSEVESYDS
                     EINEIIPDEDLDEDVEINEEEEEEEEEEEEEEEV"
     gene            12553..14157
                     /locus_tag="VBC94_001056"
     CDS             12553..14157
                     /locus_tag="VBC94_001056"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEO6979333.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLKKE
                     RRGEYLGATVQVIPHVTDALKEKIKRAATTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAG
                     QSIKNKLAQFCDVAPEAVIESLDVDHIYQIPLNMQAQNMDQIVCDHLKLETPAADMTE
                     WSAMVDKVMNLEKKVKIALVGKYVELPDAYLSVVEALKHSGYVNDVAIDLKWVNAAEV
                     TEDNIKELVGDADGIIVPGGFGQRGSEGKIEAIRYARENDVPMLGVCLGMQLTCVEFA
                     RNVLNLHGANSAELDPKTPFPIIDIMRDQIDIEDMGGTLRLGLYPCKLKAGSRAAAAY
                     NNQEVVQRRHRHRYEFNTKFREQFEAAGFVFSGVSPDNRLMEVVELPEKKFFVAAQYH
                     PELQSRPNHAEELYTAFVTAAVENMK"
     gene            14266..15192
                     /locus_tag="VBC94_001057"
     CDS             14266..15192
                     /locus_tag="VBC94_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6979334.1"
                     /translation="MKKIRLSKFIKMIVVILFLISVAASFYFFHVAQVRDDKSFISNG
                     QRKPGNSLYAYDKSFDKLLKQKIEMTNQNIKQVAWYVPAAKKTHKTVVVVHGFANSKE
                     NMKAYGWLFHKLGYNVLMPDNIAHGESHGQLIGYGWNDRENIIKWTEMIVDKNPSSQI
                     TLFGVSMGGATVMMASGEKLPSQVVNIIEDCGYSSVWDELKFQAKEMYGLPAFPLLYE
                     VSTISKIRAGFSYGQASSVEQLKKNNLPALFIHGDKDNFVPTSMVYDNYKATAGKKEL
                     YIVKGAKHAKSFETEPEKYEKRISSFLKKYEK"
     gene            15239..15324
                     /locus_tag="VBC94_001058"
     tRNA            15239..15324
                     /locus_tag="VBC94_001058"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15273..15275,aa:Leu,seq:aag)
     gene            15387..15833
                     /locus_tag="VBC94_001059"
     CDS             15387..15833
                     /locus_tag="VBC94_001059"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEO6979335.1"
                     /translation="MSKVRGFELVSQFSNKELLPKRETAHAAGYDLKVAKKTVIEPGE
                     ITLVPTGIKAHMQPGEVLYLYDRSSNPRKKGIVLINSVGVIDGDYYNNQVNEGHIFAQ
                     MQNITDQAVILEEGERIVQAVFAPFLLADDDQATGMRTGGFGSTGK"
     gene            15995..17359
                     /gene="radA"
                     /locus_tag="VBC94_001060"
     CDS             15995..17359
                     /gene="radA"
                     /locus_tag="VBC94_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEO6979336.1"
                     /translation="MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKN
                     ARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
                     KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFGNAKRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQESFIGE
                     IGLTGEIRRVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDVIGVTTVSQVLKA
                     VFST"
     gene            17495..17992
                     /locus_tag="VBC94_001061"
     CDS             17495..17992
                     /locus_tag="VBC94_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEO6979337.1"
                     /translation="MTTYFDNFLKTNQAYADLHGTAHLPIKPKTKVAIVTCMDSRLHV
                     AQALGLALGDAHILRNAGGRVTADVLRSLVISQQQLGTREIVVLHHTDCGAQTFTNEA
                     FAAQLQRDLGVDMHGHDFLPFNDIEESVREDVAKLHASPLIPNDVVISGAIYDVDTGR
                     MVEVK"
     gene            18146..19465
                     /locus_tag="VBC94_001062"
     CDS             18146..19465
                     /locus_tag="VBC94_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEO6979338.1"
                     /translation="MKKYDVIVLGFGKAGKTLAAKLATQGKSVAMVEEDDKMYGGTCI
                     NIGCIPTKTLLVSASKNHDFQEAMTTRNEVTSRLRAKNFAMLDNKDTVDVYNAKARFI
                     SNKVVELTGGADKQELTADVIIINTGAKSVQLPIPGLADSQHVYDSTAIQELAHLPKR
                     LGIIGGGNIGLEFATLYSELGSKVTVIDSQSRIFAREEEELSEMAQDYLEEMGISFKL
                     SADIKSVQNEDENVVISFEDEKLSFDAVLYATGRKPNTEGLALENTDIKLTERGAIAV
                     DEYCQTSVENIFAVGDVNGGPQFTYISLDDSRIVLNYLNGDKDYSLKNRGAVPTSTFT
                     NPPLATVGLDEKTAKEKGYQVKSNSLLVSAMPRAHVNNDLRGIFKVVVDTETNLILGA
                     RLFGAESHELINIITMAMDNKIPYTYFQKQIFTHPTMVENFNDLFNF"
BASE COUNT         6405 a         3032 c         4129 g         5944 t
ORIGIN      
        1 aatgacggaa atcattgatt tgtatgattt aacaagatat gataagtgat attagcactc
       61 tttgaataag agtgctaatt tttgatgttt tttccttgac tatcttttaa aaaggagtat
      121 aataaaatca tagttagcag tcgatgtgta agagtgctaa tttcagaaag accgagaggg
      181 gtgatgtttt tgtgattaca caacgtcaaa atgatatttt gaatttaatc gttgagttat
      241 ttacacaaac tcatgaacca gtagggtcta aagctttaca gagaacgatt gattctagta
      301 gcgccactat tcgtaatgat atggcaaaac tagagaagtt ggggttattg gaaaaagcac
      361 acacttctag tggacggatg ccgagtccag ctggttttaa gtattttgta gagcactccc
      421 ttagactaga tagtattgat gagcaagata tttatcatgt tataaaagct tttgactttg
      481 aagcattcaa attagaagac atgcttcaaa aagctagtca tattttatcg gagatgacgg
      541 gctatacatc cgttatttta gatgtggagc cagctcgtca acgtttgaca gggtttgatg
      601 ttgttcagct tagcaaccat gatgctctag cagtcatgac tttagatgaa tctaagccag
      661 tgacagtgca gtttgctatt ccaagaaact ttttgacacg tgacttgata gcatttaaag
      721 ctatcgtaga ggagcgcttg ctggatggta gtgtcatgga tattcattat aagttgagga
      781 cagaaattcc tcagatcgtt cagaagtatt ttgtaactac ggataatgtt ttacaactat
      841 ttgattatgt tttttcagag ctgtttttgg aaacggtatt tgtagctggc aaggtaaatt
      901 ctttaacata ttcagatcta tcaacgtatc agtttttgga taatgagcag caagtagcaa
      961 tttctttgag gcaaggtctg aaagagggtg agatggcttc tgtccaagtg gcagatagtc
     1021 aagaagccgc acttgcagat gtgtcagttt taactcacaa atttttgatt ccttaccgtg
     1081 gatttggact cctgagtttg attggtccta ttgatatgga ttatcgtagg tctgttagtt
     1141 tggtaaacat tatcggaaag gtattggctg ctaaattagg tgattattat cgatacctaa
     1201 attctaacca ttatgaagtt cactgataat ggcagtattc aataagttat ttaaaaggag
     1261 gcactcagtg tcagaagaaa ttaaaaaaga cgatttacag gaagaagtgg aagccactga
     1321 aactgaagag atagttgagg aagttattga agaaattcct gaaaaatcag agttagaact
     1381 cgcaaacgag cgtgctgatg agtttgaaaa taaatacttg cgtgctcatg cagaaatgca
     1441 gaatatacag cgtcgctcta gtgaagaacg tcaacaactt caacgctatc gttctcagga
     1501 tctggcaaaa gcaatcttac catcgcttga caatctagaa cgtgccttgg ctgttgaagg
     1561 gttgacagat gatgttaaaa aaggtttaga aatgactcgt gatagcctta ttcaagcact
     1621 aaaggaagaa ggcgtagaag aagtagaagt cgactcattt gaccacaact ttcatatggc
     1681 agttcagacc ctcccagccg atgatgaaca tccagccgat agcattgcag aagtctttca
     1741 aaagggatat aaattgcacg agcgtctgct aagaccagcg atggtagttg tctataacta
     1801 aggtaaatga gttttcgttt ttgtccgtaa tgacagtaaa ctagatagca agttagaagc
     1861 tattcagctt gctgattaaa ctatagtgat tgcttagaat tggaagtaaa ataattcgag
     1921 tgcttactaa gataaattga aataaaaagt aataaagtat tataaaataa gaggtattaa
     1981 catgtctaaa attattggta ttgacttagg tacaacaaac tcagcagtag cagttcttga
     2041 agggactgaa tcaaaaatca ttgctaaccc agaaggcaat cgtacaactc cttcagtagt
     2101 atcattcaaa aatggtgaga ttatcgtggg tgatgctgca aaacgtcaag cggtaacaaa
     2161 tccagatact gttatctcta tcaaatcaaa gatgggaact tctgaaaaag tttctgcaaa
     2221 tggtaaagaa tatactcctc aagaaatttc agcaatgatt cttcaatacc ttaaaggtta
     2281 tgctgaagac tatcttggag aaaaagtaga aaaagcagtt attactgttc cagcttactt
     2341 caacgatgca caacgtcagg caactaaaga cgctggtaaa attgcaggtc ttgaagtaga
     2401 acgtatcgtt aacgaaccaa cagcagccgc acttgcttat ggtatggaca agactgacaa
     2461 ggatgaaaaa atcttagttt ttgaccttgg tggtggtaca tttgacgtat caatccttga
     2521 attaggtgat ggtgtcttcg acgttcttgc aacagcaggt gataacaaac ttggtggtga
     2581 cgactttgac cagaaaatta ttgatttctt ggtagaagaa ttcaagaaag aaaatggtat
     2641 tgatctttct caagacaaaa tggctcttca acgcttgaaa gatgctgctg aaaaagctaa
     2701 aaaagacctt tcaggtgtaa ctcaaactca aatttcatta ccgttcatca ctgctggttc
     2761 tgctggtcct cttcacttgg agatgagctt atcacgtgct aaatttgacg atctcactcg
     2821 tgaccttgtt gaacgtacga aaactccagt tcgtcaagct ctttcagatg caggcttgtc
     2881 attgtcagaa attgatgaag ttatcctcgt tggtggatca acacgtatcc cagcagttgt
     2941 tgaagctgta aaagctgaaa ctggtaaaga accaaataaa tctgttaacc ctgatgaagt
     3001 ggttgccatg ggtgctgcta tccaaggtgg tgttatcact ggggatgtga aagacgttgt
     3061 acttcttgac gtaacaccat tgtcacttgg tattgaaaca atgggtggtg tcttcactaa
     3121 attgatcgac cgcaacacaa ctatcccaac atctaaatca caagtcttct caacagcagc
     3181 agacaaccaa ccagccgttg atatccatgt tcttcaaggt gaacgcccaa tggcagcaga
     3241 taacaaaaca ctcggtcgct tccaattgac tgatatccca gctgcacctc gtggaatccc
     3301 acaaattgaa gtaacatttg atatcgataa aaatggtatt gtatctgtta aagctaaaga
     3361 tctcggtact caaaaagaac aacacattgt tatccaatct aattcaggat taactgatga
     3421 agaaattgat aaaatgatga aagatgctga agcaaatgct gaagcagatg caaaacgtaa
     3481 agaagaagtt gatcttaaaa atgaagttga ccaagccatc tttgcaacag aaaaaactat
     3541 taaagaaact gaaggtaaag gctttgatac agaacgcgat gcagcgcaat cagcacttga
     3601 tgagttgaaa aaagctcaag aatcaggtaa ccttgacgac atgaaagcta aacttgaagc
     3661 tcttaacgaa aaagcacaag ctcttgcagt taaactttac gaacaagcag ctgcagcaca
     3721 acaagcagct caaggggctg aaggtgcaca atcagctgat tcatcaagca agggtgatga
     3781 tgttgtagat ggcgaattca ctgagaaata attattaata ttgttcagat tcatttgaat
     3841 ataagcatga aaactatact agcatagtaa agttcttcgt gataggggtt gctcaataat
     3901 ctagataagt ttcagattac ataagctaat ttcgctatca ctaaataaaa acatattaat
     3961 aataaatagg cggggcgcct cgctccgtct gttttattaa gtgtcatata tatgttaact
     4021 atttagagct gtaactgggc aagaataatt gttaatctct tcaagtgtag tatatgaaca
     4081 aaatataaag gattagataa tgaacaatac agaattttat gatcgtcttg gcgtttcaaa
     4141 agatgcttct caggacgaaa taaaaaaagc ttaccgtagg atgtctaaga aataccatcc
     4201 agatattaat aaggaagcgg gagcagagga aaaatataaa gaggttcaag aagcttatga
     4261 aaccttgagt gatacacaaa aacgtgctgc ctatgatcaa tatggcgctg ctggggcaaa
     4321 tggcggtttt ggtggcttcg atggtggtgg ctttggtggt tttgaagaca tcttctcaag
     4381 cttttttggt ggaggaggga tgcgcaatcc taatgcacca cgtcaaggag atgacctaca
     4441 atatcgtgtt aatttaagtt ttgaagaagc tatctttgga gctgaaaaag aagtatctta
     4501 taatcgggag tcatcatgtc atacttgttc aggaagtgga gctaagccag gtactagccc
     4561 tgtgacttgt caaaagtgtc atggttcggg tgtgattaat gttgatacac aaacacctct
     4621 aggaacaatg cgtcgccaag tgacttgtga tgtttgtcag ggtagtggtc aagaaatcaa
     4681 agagaaatgt ccaacatgtc atggaactgg tcatgagaag aagacgcata aagtatcagt
     4741 taaaatacct gcaggagttg aaactgggca acaaattcgt ttaacaggac aaggagaagc
     4801 tggcttcaat ggtggtcctt atggtgatct ctttgttatt attaacgttc tcccaagcca
     4861 acagtttgag cgtaatggat caacaattta ttatactttg aatattggct ttgtccaagc
     4921 tgctttgggt gatacaattg atatacctac tgtccatgga gcggtagaaa tgtcaattcc
     4981 tgctggtact cagacaggaa aaacgttccg tcttcgcgga aaaggtgccc ctaaacttcg
     5041 agggggtgga caaggtgatc agcatgttac tgttaacatc gtaactccta ctaaattgaa
     5101 tgatgctcaa aaagaggcat tgcacgcttt tgcagaggca agtggagata agatggtaca
     5161 tccgaagaag aaaggtttct ttgataaagt caaagatgct ttagatgttg attaaaaaat
     5221 aaaagtctag aatcgaaagt attctagact tttttgtagt tattgctaga gggattgctt
     5281 gaacctaaat tttacagtac aatgaatcta atattgtagg gggacaattt ttatggtaac
     5341 caaagttgaa gagattcgct catatttgat agcttctata caaaatggta agttggctcc
     5401 aggagatcgc ctaccatcta taagacagtt agctaatcaa ttttcctgta acaaagatac
     5461 agtgcaacga gttttgatgg aattgcgttt tgataattat atctatgcaa agcctaggtc
     5521 aggctattac gtctttgatt ctcatcaaga ggaagttgaa gaaggggtta gtttaccaaa
     5581 ctctgagatt gcaaatatag cttatgatga ttttagattg tgtttgaatg agacccttat
     5641 tggtagggaa gattaccttt tcaattatta ctatcgtcaa gaaggtcttc ttgatttaag
     5701 taaagcagtg gctaaattaa tggaagaaac aggggtctat gttccccttg atgatattgt
     5761 tattacggct ggtactcaac aggcattatt tattttgaca caggttacct ttccaaatcg
     5821 aaaatctcga gttttaatag aagaaccgac ttatcctcgt atgattgaac taatcaaaac
     5881 acaaaattta ccctatgaaa ctattcctcg aggtactcat ggaattgatt ttcagcgctt
     5941 agaggagatt ttccagacac aatcaattaa gtttttttat gttatacctc gcatgcataa
     6001 tcctttggga acatcctata atccggtaga gatgaaaaga ttaatagaga tggcagagaa
     6061 gtatgatgtt tatattgtgg aagatgacta tatgtctgat tttgcaagtc agtcaccatt
     6121 acattattat gatactcacg ggcgtgttat ttatctaaaa tctttttcaa aggctatttt
     6181 ccctgcttta agattagctg cgatttgttt accacaagct ttaaaatcaa catttatggc
     6241 ttacaagaag ttgatggatt atgatactaa tctgatttta caaaaagcat tagcgcttta
     6301 tattgaaaat ggcctttatg ctaagaatag tcaatatttg aaatatcgtt atcagaaaga
     6361 ccttgcaaat tcaaaatcta ttttagctga tcaccctaat ctacccccat atagtttaca
     6421 tcacgatagt gtattatttg attgttcgaa actcgataac ttcaaaatat tacggcaata
     6481 cggcgatact ttggaaaatt atttttgtca aaaatcgcat caatctctct tacaagtaaa
     6541 aaatgattcc tgcttaaagc agttcttggg atcgttgtag tataattgta gggggacagt
     6601 ttaaaataga tattatagtt ttcataaatg aaaggtggta aaataaaccc atgacaaggt
     6661 ataaggcaca gatttcttat gatggttcag ctttttcagg cttccaaaga caaccaaatt
     6721 gtaggacagt tcaagaagaa attgagagaa ctcttaagcg cctaaatagc gggaacgatg
     6781 tgataatcta tggtgctggt cgcacagatg ctggtgttca tgcttatgga caagttattc
     6841 attttgattt accacaggcg cgtgatgtag aaaaattacg ctttggttta gatacacagt
     6901 gtccagacga tattgatatt gttaaagtag agcaagtgtc tgatgatttt cattgtcgat
     6961 atgataaaca cattaaaact tatgagtttt tagtagatat cggtaggccg aaaaatccta
     7021 tgatgagaaa ctatgcaaca cattaccctt atcctgtcat tattgagctt atgcaagaag
     7081 ctattaaaga tttagtaggc acccatgact ttacaggctt tactgcttca ggtacgagtg
     7141 tagaaaataa agtacgtact atttttgatg cgaaaattca gtttgaagcg tctaaaaatc
     7201 ttttaatttt tacctttaca ggaaatggtt ttttgtataa acaggtgcgt aatatggttg
     7261 gtaccctgct aaaaattggt aatggacgca tgcctattag ccaaatcaaa acaattttac
     7321 aggctaaaaa tcgggattta gcaggtccaa ctgcggcagg aaatggttta tatttgaagg
     7381 agattattta tgaagacgag gaatgtttta gcaatttcag gaaatgatat tttctctggc
     7441 ggcggcttgc atgcagattt agcaacttac gttgttaata aattacatgg ttttgtggca
     7501 gtaacatgct taacagcaat gtcagataaa gggtttgaag tcattcctat agaggcatct
     7561 atactaaagc agcaactaga aagtttaaaa gatgtagagt ttgggtctat taaactagga
     7621 ttgcttccca atgtagaaac agcacaagtt gttttggaat ttgtaaaatc aaaacaagaa
     7681 tgtccagtag tcttagaccc agtcttagtt tgtaaagaaa atcatgattt agaagttagc
     7741 caattacgtg agcagctaat tgcgtttttc ccatatgctg atgtgattac tcctaattta
     7801 gtagagactc aattattgac gggtctttca attgaaaact tagatcaaat gaaaattgct
     7861 gctgagaaac tttacgatat gggggctaaa catgttgtta tcaaaggggg caaccgttta
     7921 aatgctgaag aggctacaga cctttactat gatggtgaac gttttgaaac ctatgttttt
     7981 cctgtagtag atgccaataa tacaggtgct ggatgtacat ttgcgtcaag tattgccagc
     8041 caattagcta tgggtaaaaa tgtagaagat gcagtcaaaa tgtctaaagg tttcgtctat
     8101 caagctatta aagcatcgga taagtacgga gttgtccaac atttttagga gattttaatg
     8161 aggaaagaga aaactagtca attggttcaa ttggcaattg taacagctat atctattgta
     8221 ttggggatgt ttataagtat tccgacacca acaggttttt tgacattact agatgctgga
     8281 atattctttg ctgcctttta ctttggcaaa aaagaagggg ctgtggtagg tgccttagct
     8341 ggttttttaa ttgatttatt gaaaggttat cccaattgga tgttctttag tttattgatt
     8401 catggggcgc aagggtattt agcgggttta ccaggtagaa gacgtctttt aggtcttatt
     8461 agtgcaactc ttgttatggt attaggttat gctatagcta gtggattgat gtatggttgg
     8521 ggagctgttt taccagatat tcctggcaat attatgcaaa atatggttgg tatggtcgtt
     8581 ggttttgctc ttaataagtc tctagaaaga gtgaagaaat gatactagat tataataagt
     8641 taaagcagga aactaaagct attgtagttg atattattga gcgtagtgct cttaaaaaag
     8701 gtcaaatttt tgtactaggt ctatcatcta gtgaggtttc tggtggttta attggaaaaa
     8761 attctagttc tgaaattggt gaaattatcg ttgaagttat cctcaaagag ttacattcac
     8821 gcggcatcta tttagcagtt caaggttgtg aacatgttaa tagggcgttg gttgttgaag
     8881 cagaattggc tgaacgtcaa cagttggaag tcgttaatgt tgttcctaat ctacatgctg
     8941 gcggtagtgg tcaggttgca gcttttaagt tgatgacatc accagttgag gttgaagaaa
     9001 ttgtggcaca tgctggaatt gatattggag atacttctat tggaatgcat atcaagagag
     9061 tacaagttcc cttgattcca attagtcgtg agttgggagg ggcacacgtt acagcactag
     9121 ctagtcgtcc taaattaatt ggcggtgcta gagcaggata tacgagcgat ccaatccgta
     9181 aattttaaaa taaacctctg ctatgcagag gtttattgtt tatctcaacg agagtggtta
     9241 ttggagaaat tgtatgttgg taagtcaata ccttcttcaa gaatagcttt ttgataaagt
     9301 ttgtaaaatt cagcatagat gtcaaactga gcaccgtttt gcgtgaagat attaatacga
     9361 taaacaaatt gaccattagc attggttgta ggaccaaaaa cagtaggctc tccaacgata
     9421 gcaggatgtt ttgaaacata ctcttcattg atttttgtta caatatcaga aatctgatca
     9481 aggtttgtat gaacaaataa aggaatatca atttgagcac gcatattgct acgagactta
     9541 ttgctgacaa ttgtaatatt acgattagga atgaagtgta aggttccatc gaaatcatga
     9601 atttgtgtgg tgcgtatccc aacattagta acagtgcctg atacagcacc aacattaata
     9661 atatcaccaa catcaaattg gttttctagc agaataaaaa agccattgac aacatctgat
     9721 aagaagcctt gagcaccaag tccaatagca accccagcaa taccagcccc agctaataaa
     9781 ctagaaattg gaacgcccaa tatacttaaa atccaataga atagaaagaa ataaagggta
     9841 tagtttaaaa cattgtgaga aagtttagct aatgtttttt gacgagctac tttttgtcta
     9901 gaaaaggcga gtgagcgatt aacagtcttt tcgaaaagat aattgatgac ttgcctaacg
     9961 ataacgaatg caatgattaa taataagatt gatattagtt tggtgaagaa agtaaataaa
    10021 acttcctcta cattaagatg gtcaataaat ttttctaaag taatcatagt aatagtctag
    10081 taaaaaaaaa gccatagagc aagtatttta aaagcttatc gttaagcagt ttaaagcttc
    10141 ttttttgcat aaaaactttg taaaacgttt taaattatgg taaactaaac tgtatctaaa
    10201 taattttaag gagaaatgac ttaatgtcta catcatttga aaataaagct acaaaccgtg
    10261 gtattatcac atttacaatt agtcaagatg agatcaagcc agctcttgat caagcattca
    10321 acaaagttaa aaaagatttg aacgttccag gattccgtaa aggacatatg cctcgtacag
    10381 ttttcaacca aaaatttggt gaagaagcac tctacgaaaa tgcattgaat ttagtgttgc
    10441 caaaggctta cgaagcagct gttgcagaac ttggtttaga cgttgttgct caaccaaaaa
    10501 ttgatgttgt atcaatggaa aaaggtcaag attggaagtt gacagctgaa gttgttacaa
    10561 aaccagaagt taaacttggg gattacaaag acctttcagt agaagttgat gcttcaaaag
    10621 aagtttcaga tgaagaagtt gatgctaaag ttgaacgtga gcgtaacaac cttgcagaat
    10681 tgactgttaa agatggtgaa gcagcacaag gtgatacagt tgttattgac tttgtaggtt
    10741 cagttgatgg tgttgaattt gatggtggta aaggagataa cttctcactt gaacttggtt
    10801 caggtcaatt tatcccagga tttgaagaac aattagtagg ttctaaagct ggtcaaactg
    10861 tagacgttaa tgtaacattc ccagaagatt atcaagcaga agatcttgct ggtaaagatg
    10921 ctaaatttgt tacaacaatt catgaagtaa aaactaaaga agttccagca cttgatgatg
    10981 aacttgcaaa agatattgat gatgaagttg aaacacttga tgagttgaaa gcgaaatatc
    11041 gcaaagagct agaatcagca aaagaaattg catttgatga tgcagttgaa ggtgtagcaa
    11101 tcgaattagc tgttgccaat gcagaaattg ttgaattacc agaagaaatg gtccatgatg
    11161 aagtacatcg tgctatgaat gagttcatgg gtaatatgca acgtcaagga atttcaccag
    11221 aaatgtactt ccaattaaca ggtacaactg aagaagacct tcataagcaa taccaagcag
    11281 atgctgacaa acgcgttaaa actaaccttg ttattgaagc aattgcagct gcagaaggct
    11341 ttgaagcaac agatgaagaa atcgaaaaag aaatcacaga tcttgcttca gaatataata
    11401 tggaagctga ccaagtacgc ggtctacttt cagcagatat gcttaaacat gatattgcaa
    11461 tgaaaaaagc ggttgacgtt atcactagct cagctactgt aaaataattg cttattaaat
    11521 tttgaaaaga ttgggcttcc cagtcttttt atttgtgatt attcttttga cgaatagcta
    11581 aaaatagcgt aaaatagtaa atagcgaaaa tttgaaaata tgacttatct attttctatc
    11641 tataaagaat attgctaaac aaaatgatag tcttacacaa ttaaggagaa taactttgga
    11701 attagaagta tttgctgggc aagaaaaaag tgaactttcg atgattgaag ttgctcgcgc
    11761 tattttagag caacgtggtc gtgataacga aatgtacttc agcgatttgg taaatgatat
    11821 tcaaacctac cttggaaaat ctgactcagc aatccgtgag tcattgccat ttttctattc
    11881 agatttgaat acagacggaa gttttattcc acttggcgaa aacaaatggg gacttcgttc
    11941 atggtatgct atcgatgaaa ttgacgagga aatcatcact cttgaagaag atgaagatgg
    12001 cgctccaaaa cgtaagaaga aacgtgtcaa tgctttcatg gatggtgatg aagatgctat
    12061 tgattacaat gatgatgatc cagaagatga agatttcaca gaagaaacac caagtttaga
    12121 atatgatgaa gaaaaccctg acgatgaaaa atcagaggta gaatcgtatg attctgagat
    12181 taatgaaatc attcctgatg aagatttaga tgaggatgta gaaatcaacg aagaagagga
    12241 agaagaggaa gaagaggaag aagaggaaga agaggtttag atggggattt tgtatttgac
    12301 aaatagtcag atatagttta aactattatt cgggcacctc ttatgaggtc agataaagct
    12361 ccctagtgat agggggctat tttgtttttg ggaaagatac agaatggttc cagatagttt
    12421 tctgagtagg cccttgatca gatagtttct actagttttg actagagaag ctggtattat
    12481 ttatcaatca tgtgttttgg gttttacccg taaactaact gttagattaa catttaattt
    12541 aaggagtttt taatgacaaa gtatattttt gttacaggtg gtgtggtctc ttctattggt
    12601 aaggggattg ttgcagcaag tcttgggcgc ttactaaaaa accgtgggct aaaggtgaca
    12661 attcaaaaat ttgatccata tattaatatt gacccaggga caatgagccc ttatcaacat
    12721 ggggaagttt atgttacaga tgatggtgca gaaactgacc ttgaccttgg acactacgag
    12781 cgttttattg atattaactt aaacaagtat tcaaatgtta caacgggtaa gatttatagt
    12841 gaagttctta aaaaagaacg ccgtggagaa taccttggtg caactgttca agttatccca
    12901 cacgttacgg atgctttgaa agaaaaaatc aaacgtgctg cgacaactac agattcagat
    12961 gttattatta ctgaagttgg cggtactgtt ggagatattg agagccttcc gtttttagaa
    13021 gcacttcgtc aaatgaaggc agatgttggt tctgataatg ttatgtacat tcatacaacg
    13081 ttattaccat acctaaaggc tgccggcgaa atgaaaacaa aaccgactca acattctgtt
    13141 aaagagttac gtggcctagg tatccaacct aatatgcttg ttatccgtac agagcaacca
    13201 gcaggtcaaa gtattaagaa taaattggca caattttgtg atgttgcacc agaggctgtt
    13261 atagagtccc ttgatgttga ccatatttat caaattccac ttaacatgca agctcaaaat
    13321 atggatcaaa ttgtttgtga tcacttgaag cttgagacac ctgctgctga tatgacagaa
    13381 tggtcagcaa tggttgataa agtaatgaac cttgagaaaa aagttaaaat tgcattggtt
    13441 ggtaaatatg ttgaattacc tgatgcttac ctttcagttg tggaggccct taagcactca
    13501 gggtacgtca acgatgttgc tattgaccta aaatgggtaa atgctgctga agtgacagaa
    13561 gataatatta aagaacttgt cggagatgca gatggtatca tcgttcctgg tggatttggc
    13621 caaagaggta gtgagggtaa aattgaagca attcgctatg cacgtgaaaa tgatgttcca
    13681 atgcttggtg tttgtctcgg aatgcagcta acttgtgtcg aatttgcgcg caatgtttta
    13741 aaccttcatg gagctaattc agcagagctt gaccctaaaa caccgttccc aatcattgat
    13801 atcatgcgtg atcaaatcga tattgaagat atgggtggta cacttcgcct tggtctatat
    13861 ccatgtaaat tgaaagcagg ttcaagagct gcggcagctt ataataatca agaagttgtc
    13921 caacgtcgtc accgtcaccg ttatgaattt aatacaaaat tccgtgagca atttgaggct
    13981 gctggatttg tcttctcagg ggtatcaccg gataatcgtt tgatggaagt tgttgagtta
    14041 ccagagaaaa aattctttgt tgcagcacaa taccatccag aactacaatc acgtcctaac
    14101 catgctgaag agctttacac agcttttgta acagcagcag ttgaaaatat gaaataactt
    14161 tatcgcctcc cagtattagg aggcgatttt atttttttag atagtttcac tatcaaagta
    14221 ttattagctc ttttcttttt ctaaaaataa ggtataatag gttgcatgaa aaagattcga
    14281 ttatcaaagt ttattaaaat gattgttgtt attttgtttt taattagtgt agcagctagt
    14341 ttttattttt tccacgttgc ccaagttcga gatgataaat cctttatttc aaatggtcaa
    14401 cgtaagcctg gaaactcttt atatgcttat gataaatcct ttgataagct attaaagcaa
    14461 aaaatagaaa tgacaaacca aaatataaag caagttgctt ggtatgttcc tgctgctaag
    14521 aaaactcata agacagttgt tgtcgttcat ggttttgcga atagcaaaga gaatatgaag
    14581 gcatatggtt ggctgtttca taagttagga tacaatgttc ttatgcctga caacattgca
    14641 catggtgaaa gtcatgggca gttgataggc tatggctgga acgaccgcga gaacattatc
    14701 aaatggacag aaatgatagt ggataagaat ccatcaagcc aaattacttt atttggtgtt
    14761 tcaatgggtg gagcaacagt catgatggct agtggtgaaa aattacctag tcaggttgtt
    14821 aatatcattg aagattgtgg ttattctagt gtttgggatg aattaaaatt tcaggctaaa
    14881 gagatgtatg gtttaccagc cttcccactc ttatatgaag tttcaacaat ttctaaaatc
    14941 agagcaggtt tttcgtatgg acaagcaagt agtgtcgaac aattgaaaaa gaataattta
    15001 ccagccctct ttattcatgg tgataaggat aattttgttc caacaagtat ggtttatgac
    15061 aactataaag ctacagcagg taagaaagag ctttatattg taaaaggggc aaaacatgcg
    15121 aaatcttttg aaacagagcc agaaaaatat gagaaacgta tctctagttt tttgaaaaaa
    15181 tatgaaaaat aatattgaca acaatatgat aagttgatat aatttattat gttgttaagc
    15241 ggggatggcg gaattggcag acgcgcaaga ctaaggatct tgtgaccgct tttggtcgtg
    15301 agggttcaag tccctctctc cgcagactag tcgagttcat ttggaactcg actttttgca
    15361 tatttaagat aaagatggag tatcacatgt caaaagtacg tggctttgaa ttagttagcc
    15421 aatttagcaa taaagagtta ttaccaaaac gagaaacagc tcacgcagct ggctatgatt
    15481 taaaagtagc taaaaaaacg gttattgaac ctggtgaaat tacattggtc ccgaccggta
    15541 ttaaagcaca catgcaacca ggggaggtcc tgtaccttta tgatcgttca tcgaatccac
    15601 gtaaaaaagg gattgtccta ataaattcag ttggtgttat tgatggtgac tactataata
    15661 accaagtcaa tgaaggacat atttttgcac agatgcaaaa tattactgat caggctgtta
    15721 ttttagaaga aggggaacgg attgttcagg ctgtttttgc cccattctta ttggcagatg
    15781 acgatcaggc tacaggaatg cgtacaggtg gttttggtag tactggaaaa taaaattatt
    15841 ttacgaataa tgttagaaaa ggctagactt acttcttagc cttttttgat gagagtaaac
    15901 ttatattttc tctaacttgg tgtgatataa tgagtgtact agttatttgg taccaagcta
    15961 tcatattaat ttagaagtta taaaggaggc ctgagtggct aagaaaaaat cagtcttcac
    16021 ctgtcaagag tgtggttacc aatcacctaa gtatttggga cgttgtccaa attgctcagc
    16081 ctggtcttca tttgtagaag aggttgaagt tcaagaagtt aaaaatgcac gagttagttt
    16141 gaacggtgaa aagtcccgac cgacgaagtt aaaagatgtt tcctcaatta actattctag
    16201 aactaagact gatatggatg aattcaaccg tgttctagga ggcggtgttg taccaggaag
    16261 tttggttctg attggaggag accctgggat aggtaaatct actttattac tacaagtatc
    16321 aactcagcta gctaataaag gtacagtatt atatgtctca ggtgaggaat ctgcagaaca
    16381 gattaagtta cgaagtgagc gtctaggcga tatcgataat gaattttacc tttatgccga
    16441 gactaatatg caaagtattc ggtcggagat tgagaagatt aaaccagatt ttcttattat
    16501 tgattctata caaactatta tgagtcccga agtatcaagt gtccaaggct ctgttagcca
    16561 agttcgcgaa gtgaccgctg agttgatgca gttagctaaa actaataata ttgctacttt
    16621 tattgtaggt catgtgacta aggagggaac attagcaggc cctcgaatgc tagaacatat
    16681 ggtggatacg gttctttatt ttgaagggga gcgtcatcac acgttccgta ttttacgggc
    16741 agtcaaaaac cgttttggtt cgaccaatga aataggtatc tttgaaatgc agtcgggtgg
    16801 tctagttgaa gttttgaatc caagtcaagt ttttcttgaa gaacgtttag acggtgcaac
    16861 aggatctgct attgttgtta ctatggaagg aacaaggcct attttagcag aggttcaagc
    16921 tttagtgact ccaactgttt ttggaaatgc taagcgcaca accacaggtc ttgactttaa
    16981 tcgtgtcagt cttattatgg cagttctaga aaaacgttgt ggtttattgc ttcaaaatca
    17041 agatgcctat ttaaaatcag cgggtggtgt taaattggat gaaccagcaa ttgatttagc
    17101 tgttgctgta gctattgctt ccagttacaa agaaaaaccc acaaatccac aagaatcatt
    17161 tattggagaa attggactca ctggtgagat tcgtcgcgtg acacgtattg agcaacgtat
    17221 taatgaggct agtaaattgg gctttactaa aatttacgca ccaaagaatt ctctagctgg
    17281 gattgaaata cctaaaggca ttgatgttat tggtgtgaca actgttagtc aggtcttaaa
    17341 agcagtgttt tcaacataat gattagattc ggttcagccg aatctttttt ggtggcgaga
    17401 gacatttcgt gaaaaataat gggcaaaatg ataatataaa agggttggta tttagtaaat
    17461 atcgaatgat gttacactaa caaaggagat ctctatgacc acttattttg ataacttttt
    17521 aaaaaccaat caagcctatg ctgacttaca tggaacagct catctaccta tcaagccaaa
    17581 gacaaaggtt gctattgtaa cctgtatgga ttctcgttta cacgttgctc aagctctagg
    17641 gctagcttta ggagatgctc atattttacg taatgctggt ggacgagtta cagccgatgt
    17701 tctacgttct ttagttattt cgcaacaaca attaggaaca cgagaaatcg ttgttctaca
    17761 ccatacagat tgtggagcac agacgtttac taacgaagct tttgctgctc aattacagag
    17821 agatttgggt gttgatatgc atggacatga ttttcttcct tttaatgata ttgaagaaag
    17881 cgttcgtgaa gatgtggcaa aattacatgc ttctccatta atcccaaatg atgttgttat
    17941 ttctggtgct atttatgatg tagatactgg tcgtatggtg gaagttaaat agaaaaaatc
    18001 ctgtccttag cgatgggttt ttttatttta gaaaactaga tttaaaggta acagttaggc
    18061 ttatcatgcc ttatgctgga attcagacag aggaaacatc ttttgctata ctgaaactat
    18121 attataaaag cggaggaaat ttaaaatgaa aaaatatgat gtcattgttc ttggttttgg
    18181 taaagcaggt aaaacattag cagcaaaatt agcaacacaa ggtaaaagcg ttgcaatggt
    18241 cgaagaagac gataaaatgt atgggggaac atgtatcaac attggatgta ttccgacaaa
    18301 aacgctttta gtatcagcta gcaaaaatca cgattttcaa gaagctatga ctactagaaa
    18361 tgaagttaca agtcgtttac gtgctaaaaa ttttgctatg ttggataata aagacactgt
    18421 ggatgtttat aatgctaaag ctcgctttat atcaaataaa gtggtcgaat taacaggagg
    18481 agctgacaaa caagaactta cagctgatgt tattattatc aatactggtg ctaagtcagt
    18541 acaattacca attccaggat tagctgattc tcaacatgtt tatgattcaa cggcaatcca
    18601 agaattggct catcttccta aacggttagg tatcattgga ggcggtaata ttgggcttga
    18661 atttgcgact ttatattccg aattagggag caaagtgaca gttattgata gtcaatctcg
    18721 tatctttgcg cgtgaggaag aggaattatc agagatggct caagattatt tagaagagat
    18781 ggggatatct tttaaattat cagccgatat caaatctgtg caaaatgaag atgaaaacgt
    18841 tgttattagc tttgaggatg aaaaattaag ctttgatgca gtattatatg caacaggacg
    18901 taagccaaat acagaaggtt tagcacttga aaatacagat attaaattga cagaacgtgg
    18961 agctattgct gttgatgaat attgccaaac ttctgttgag aatatttttg cagttggtga
    19021 tgttaatggc ggccctcaat tcacatatat ttctttagat gactcacgta ttgtcttaaa
    19081 ttatttgaat ggtgataagg attattctct aaaaaatcga ggagcagtac cgacttcaac
    19141 ctttacaaat cctcctttag caacggtagg tctagatgaa aagaccgcta aggaaaaagg
    19201 atatcaagtt aaatctaata gccttcttgt ttctgccatg ccacgtgcac atgttaataa
    19261 tgaccttaga ggtattttta aagttgtggt agatactgag acaaatctta ttttgggagc
    19321 tcgcttattt ggtgcagaat cccatgaatt aattaatatc atcacaatgg caatggataa
    19381 taaaatccca tatacttatt tccaaaaaca aattttcact cacccaacaa tggtagagaa
    19441 ttttaatgat ttgtttaact tttaagaata tttttaaaaa gtaaggcaat tttgctttac
    19501 tttttttatc
//
LOCUS       DATROQ010000034        18880 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.034, whole genome shotgun sequence.
ACCESSION   DATROQ010000034 DATROQ010000000
VERSION     DATROQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18880
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.034"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            34..996
                     /locus_tag="VBC94_001063"
     CDS             34..996
                     /locus_tag="VBC94_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO6979339.1"
                     /translation="MKKLLTTPVMGCTLVILSLTSLFVGVKSIPLEQITHLNQSQVDI
                     FLASRLPRTISILISGASLSVCGLLMQQLTQNKFVSPTTSGTMDWAKLGVVVTLIFFK
                     NTSIFIQLCIASGFAILGSLLFVTILKMITFKDNIFIPLIGLMLGQIVAAATVFLGTH
                     FQVLQSVNSWLQGNFSIMTSHRYEILYLALPCLFLVYFFAHQFTIVGLGESFAKNLGI
                     AYEKMIYFGLVLVSIMTSLVIIIVGALPFLGLIVPNLISLTKGDHMSSTILETSLLGA
                     CIVMICDLFGRLVIFPYEVSIGVTLGVLGSAFFLISIIRNEGKS"
     gene            993..1967
                     /locus_tag="VBC94_001064"
     CDS             993..1967
                     /locus_tag="VBC94_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO6979340.1"
                     /translation="MIKDLEIPKLLILLILLIATIILFLIYGIPTDANEFLIIYILKT
                     RYQKLIALILVGICIGSSSLIFQTLTNNRLLTPSIIGLDSLYVLIQTGLMYLIGAQRV
                     IKFSSFSSFLLSLLLMVGFAYLLFTILFRNKKQSLNFVLLAGLIFNTLFSSISSFIQA
                     IMDPNDFMILQNQLFASFNAINTKILWISFIIIVVSFVINWPFIKELDVLLLGKENAI
                     SLGISYQKLTTRIFLWLALMVAIATALVGPITFLGLLVAHITYHSFHTFRHQILVPIA
                     IVICIFTLVLGQHLVQNLLHLTVQLSVLLNLIGGSYFIFTLIKGRKNL"
     gene            1964..2725
                     /locus_tag="VBC94_001065"
     CDS             1964..2725
                     /locus_tag="VBC94_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6979341.1"
                     /translation="MIQINNLHKFYGQKEILKDINISIPKGKVTAILGPNGSGKSTLL
                     SCISRLEPYDNGEIFLDKVPLAHYSSNDLAKTLAILRQSNHLTLKIKVSDLIGFGRFP
                     YSKGRLSQKDKAVIESVISYMDLNDIADEFINNLSGGQIQRAFIAMTMAQDTQYICLD
                     EPLNNLDMKYAVQMMDLIKRYAYEFNKTIVIIIHDINFATHYADNVVALKEGQVVSCG
                     TVEDVMQEKILSHLFDMPIRIETVDGKPIILFCTT"
     gene            2787..3815
                     /locus_tag="VBC94_001066"
     CDS             2787..3815
                     /locus_tag="VBC94_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6979342.1"
                     /translation="MTKKLIIAILALCTILTTSQAVLAKEKSQTVTIKNNYSVYIKKE
                     KRDKPDNKKQISETLKVPLKPKKVVVFDMGALDTITALGAEKSVIGIPKAKNALSLLP
                     NNVKSVYKAKRYQDVGSLFEPNFEAIARMQPDVVFLGARMASVDNIEKLKEAAPKAAL
                     VYAGVDSKKVFDKGVAERVTMLGKIFDQNKKAKTFNKDIAQAVLKLQKTIEKKGKPTA
                     LFVMANSGELLTQSPSGRFGWIFSVGGFKAVNENEKLSSHGTPVSYEYIAEKNPNYLF
                     VLDRGATIGQGASSKELFNNDVIKATDAVKNKRVHEVDGKDWYINSGGSRVTLRMIKD
                     VQNFVDNR"
     gene            3953..4795
                     /gene="dprA"
                     /locus_tag="VBC94_001067"
     CDS             3953..4795
                     /gene="dprA"
                     /locus_tag="VBC94_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEO6979343.1"
                     /translation="MNHFELFKLKKAGLTNLNINNIINYLKKNSLTSLSVRNMAVVSK
                     CKNPTFFIENYKQLDLKKLRQEFKKFPVLSILDSNYPLELKEIYNPPVLLFYQGNIEL
                     LSKPKLAVVGARQASQIGCQSVKKIIKETNNQFVIVSGLARGIDTAAHVSALKNGGSS
                     IAVIGSGLDVYYPTENKKLQEYMSYNHLVLSEYFTGEQPLKFHFPERNRIIAGLCQGI
                     VVAEAKMRSGSLITCERALEEGREVFAIPGNIIDGKSDGCHHLIQEGAKCIISGKDIL
                     SEYQ"
     gene            4890..7010
                     /gene="topA"
                     /locus_tag="VBC94_001068"
     CDS             4890..7010
                     /gene="topA"
                     /locus_tag="VBC94_001068"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEO6979344.1"
                     /translation="MATTTKTSTKKTSKKKSATAKKNLVIVESPAKAKTIEKYLGRNY
                     KVVASVGHIRDLKKSSMSIDFENNYEPQYINIRGKGPLINDLKKEAKKAKKVYLASDP
                     DREGEAISWHLAHILDLDKEDRNRVVFNEITKDAVKNAFVEPRQINMDLVDAQQARRV
                     LDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENEIKAFQPEEYWTIDGSFKK
                     GTRKFNATFYGLDGKKFKLSNNEDVKTVLKRIKTDEFLVEKVEKKERRRNAPLPYTTS
                     SLQQDAANKINFRTRKTMMIAQQLYEGLSLGTAGHQGLITYMRTDSTRISPLAQNEAT
                     EFITNRFGANYSKHGNKVKNASGAQDAHEAIRPSSVNHTPESIAKYLDKDQLKLYTLI
                     WNRFIASQMTAAVFDTMKVNLTQNGVTFIANGSQVKFDGYMAVYNDTDKNKMLPDMEE
                     GESVKKVNTNPEQHFTQPPARFSEASLIKTLEENGVGRPSTYAPTLETIQKRYYVKLA
                     AKRFEPTELGEIVNSLIVEFFPDIVDVTFTAEMEGKLDEVEIGKEQWQKIIDEFYKPF
                     EKELAKAETEMEKIQIKDEPAGFDCELCGSPMVIKLGRYGKFYACSNFPECHNTKAIT
                     KEIGVICPICQKGQVIERKTKRNRIFYGCDRYPECEFTSWDKPIGRTCPKSNDFLVEK
                     KVRGGGKQVVCSNEKCDYQEEKIK"
     gene            7147..7848
                     /locus_tag="VBC94_001069"
     CDS             7147..7848
                     /locus_tag="VBC94_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979345.1"
                     /translation="MSYIEMKHSYKRYQTGETEIVANNDISFSIERGELVVILGASGA
                     GKSTVLNILGGMDSNSEGEVLIDGKNIANYTIRELTRYRRYDVGFVFQFYNLVPNLTA
                     LENVELASEIVPKALDAQQALENVGLGHRINHFPAQLSGGEQQRVAIARAIAKKPKLL
                     LCDEPTGALDYQTGKQVLAILQKMAQSKETTVIIVTHNTALAPIANRVIHMHDSKISD
                     IVINENPSDIQNIEY"
     gene            7860..10490
                     /locus_tag="VBC94_001070"
     CDS             7860..10490
                     /locus_tag="VBC94_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6979346.1"
                     /translation="MGKTFWKDIYRSITTSKGRFSSILLLMMLGSFAFIGLKVSAPNM
                     QRTAQNYLAHHHVMDITVFNSWGLDKHDQTVLESLKGSQVEFSYFVDTTPQQNSKSYR
                     LYSNTKTISTFDLVKGRLPLNKSEIALSFQERKKYAIGDKINFKQDKNKLFSNTGPLT
                     IVGFVNSTEIWSKTNLGSSQTGDGDLDSYGVLDKTAFHSPVYTMARVTFKDLRLINPF
                     SISYKEKVAKYQEKVSRKLNIHNKIRYTKTKKESLRKIDEEEKSLLKAQKQINRLDND
                     SLAMPLSQRQAIQMKIKQDRLSLLKRTKELLKLRHNTQIMESPQIIVYNRTTFPGGQG
                     YNTFDSSTNSTSKISNLFPIILYLVAALVTLTTMTRFVEEERTNAGILKALGYSDRQV
                     IFKFIIYGFIAGTLGTTLGIIGGHYLLPRIISDIISKDLTIPNTQYHLFLNYSLLAFV
                     FSLLSIVLPVFVITRRELKEKAAFLLLPKPPAKGSKIALEYINWIWKKLSFTQKVTAR
                     NIFRYKQRMIMTIFGVAGSVALLFSGLGIQSSLKQTVNEHFGRIMPYDILLTYNTNAS
                     PPKILELLSKDSKIDKYQPIHLENLDESIPGQINKQSISLFITDKKQLLPFIYLQEAT
                     TNKSLHLNNKGIIISKKLAQFYHVNTGDFIHLSHSQTLPSRKLKITGVVNANVGHYIF
                     MTKQYYRTIFKKEAKDNAFLVKLTKHKIANNLAEKLLEINGVESLTQNALQLASVEAV
                     VRSLDGSMTILVVVSLLLAIVILYNLTNINLAERKRELSTIKVLGFYNEEVTLYIYRE
                     TIILSTIGVILGTISGTYLHRQMMLLIGSDQILFGEKVSPTTFIIPISVVVIILISLG
                     FIVNHQLKKLNMLDALKSVD"
     gene            10596..11474
                     /locus_tag="VBC94_001071"
     CDS             10596..11474
                     /locus_tag="VBC94_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6979347.1"
                     /translation="MRKSIFEETAKGKVSRHVLLTILLALILLFTADFLTYIIIELII
                     SLDFWKLEWDSDIVQLIILLATSISTVMLFMWVKFKEKRPISSLGLFKEGALTELSRG
                     WLVGSVLFGACLILTIISGSGVIETVRFTADKLIWFLIFAVGWQVQSASVEILCRGWL
                     LPVSATKYSKIVSVSISSIFFGLLHSANNHVSLISIFNLCLFGLFLSLYVILKGNIWG
                     ACGIHGAWNCVQGSVFGIEVSGEPMLSNSLVHVKTYGADWISGGKFGVEGSMITSIVL
                     IVACYWLYQKSNFPYD"
     gene            11581..12171
                     /locus_tag="VBC94_001072"
     CDS             11581..12171
                     /locus_tag="VBC94_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO6979348.1"
                     /translation="MPQNPTSNPEKKTYLLSELVDCFKGKAVPSKAEAGDIRIINLSD
                     MSPLGIDYHNLRTFQDEQRSLLKYLLQEGDVLIASKGTVKKVAIFEEQDYPVVASANI
                     TILRPTQHIRGYYLKLFFDSEEGQQALENANKGKAVMNISTKELLNIAIPSIPLFRQD
                     YLIQRYKQGLNDYKRKIARAEQEWERIQNDIRQQLS"
     gene            12205..13476
                     /locus_tag="VBC94_001073"
     CDS             12205..13476
                     /locus_tag="VBC94_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEO6979349.1"
                     /translation="MNEIKCPHCGTAFAINESEYHQLLEQIRGDAFDKEVSERLEKER
                     LILGEQAKNQLQEVVVEKDKEIAKLQYKVKQFLIEKDNLLKDNEYQLAEQLNQKDMML
                     RDLENQIDRLRLEHENSLQEALTKVERERDAIQNQLHIQEKEKDLALASVKSDYEVQL
                     KAANEQVEFYKNFKAQQSTKAVGESLEHYAETEFNKVRHLAFPNAYFEKDNTLSSRGS
                     KGDFIYREKDENDLEFLSIMFEMKNESDDTIKKHKNEDFFKELDKDRREKSCEYAVLV
                     TMLEADNDYYNTGIVDVSHKYPKMYVIRPQFFIQLIGILRNAALNTLKYKQELALMKE
                     QNIDITHFEEDLDIFKNAFAKNYNSASKNFQKAIDEIDKSIKRMEAVKAALTTSENQL
                     RLANNKLDDVSVKKLTRKNPTMKAKFDALKD"
     gene            13489..13920
                     /locus_tag="VBC94_001074"
     CDS             13489..13920
                     /locus_tag="VBC94_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6979350.1"
                     /translation="MKIRTATLDDSEKLVPLYQELGYAISLSEIQSILKVILTHSDYG
                     FLIAEDNGKLLAFVGYHKLYFFEKSGTYYRILALVVNEKHRRKGIASQLINHVKQLAK
                     TDGSEVLALNSSLKEYRQEAYHFYENLGFKKVSTGFSYYLD"
     gene            14012..14896
                     /gene="truB"
                     /locus_tag="VBC94_001075"
     CDS             14012..14896
                     /gene="truB"
                     /locus_tag="VBC94_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEO6979351.1"
                     /translation="MITGIINLKKEAGMTSHDAVFKLRKILHTKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTESGKIYEGEITLGYATSTEDSSGEVISRTPLTQSDLSEDVV
                     DHAMKSFTGPITQVPPMYSAVKVNGKKLYEYARSGEEVERPKRQITISEFRRTSPLYF
                     EKGICRFSFYVSCSKGTYVRTLAVDLGIKLGYASHMSFLKRTSSAGLSITQSLTLEEI
                     NEKYKQEDFSFLLPIEYGVLDLPKVNLTEEDKVEISYGRRILLENEADTLAAFYENRV
                     IAILEKRGNEFKPHKVLL"
     gene            14909..15841
                     /locus_tag="VBC94_001076"
     CDS             14909..15841
                     /locus_tag="VBC94_001076"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEO6979352.1"
                     /translation="MKIHYINDYKDIQAKEDCVLVLGYFDGLHLGHKALFDKAKKIAT
                     EKNLKIVVLTFNETPRLTFARFQPELLLHLTSPEKRSEKFQEYGVDELYLMNFTSHFS
                     KVSSDLFIKKYIYGLRAKAAVVGFDYKFGHNRTSGDYLARNFKGPVYIIDEISEGGEK
                     ISSTRIRQLITEGNVEKANQLLGYEFSTCGMVVHGDARGRTIGFPTANLAPINRTYLP
                     ADGVYISNVLINGKYYRAMTSIGKNITFGGTELRLEANIFDFDGDIYGETIEIFWLKR
                     IREMVKFNGIDDLVKQLKKDKEIALNWKKDSQTL"
     gene            15884..16297
                     /locus_tag="VBC94_001077"
     CDS             15884..16297
                     /locus_tag="VBC94_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEO6979353.1"
                     /translation="MITLFLSPSCTSCRKARAWLSKHEVAFEEHNIITSPLNKEELLQ
                     ILSFTENGTEDIISTRSKVFQKLAIDVDELSTSSLMELISENPSLLRRPIILDKKRMQ
                     IGFNEDEIRAFLPRDYRKQELKQATIRAEIEGKHD"
     gene            16290..16571
                     /locus_tag="VBC94_001078"
     CDS             16290..16571
                     /locus_tag="VBC94_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEO6979354.1"
                     /translation="MISSNYSYPLDPSWNTEDITKVLRFLNQVEHAYENSVKVDDLLD
                     SYKEFKKFVKSKAQEKQIDREFQRTSGYSTYQAVKAAQQQAKGFISLGR"
     gene            16561..17325
                     /locus_tag="VBC94_001079"
     CDS             16561..17325
                     /locus_tag="VBC94_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEO6979355.1"
                     /translation="MDAKFDFAKQLVYKAGQFIKSEMQNTFDVEEKSRFDDLVTSLDK
                     KTQKLLIQEIIQHYPDDNILAEEDDVRSPIAQGNVWVLDPIDGTVNFIVQKDNFAVML
                     AYYEEGVGQFGIIYDVMADILYSGGGHFDVYANDKKIVPFQECPLERCLLGVNSAMYA
                     ENDCGIAHLASETLGVRIYGGAGISMAKVMQGKLLAYFSYIQPWDYAAAKIMGETLGF
                     TLLTLDGEEPNYSTRQKVMFLPKSKLNLIQSYLTKK"
     gene            17393..18703
                     /locus_tag="VBC94_001080"
     CDS             17393..18703
                     /locus_tag="VBC94_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEO6979356.1"
                     /translation="MKLPNEFIEKYQTILKDEAEAFFDSFEQKPISAYRTNPLKEKQL
                     DFPNAIPSTPWGHYGKISGKSIEHTTGLVYSQEPAAQIVAQIAEPQEGMKVLDLAAAP
                     GGKTTHLLSYLNNTGLLVSNEISNKRSKILVENVERFGARNVIVTNESSQRLAKCFNS
                     FFDLIVFDGPCSGEGMFRKDPQAIQYWHKDYPTECAQLQRDILKEAIKMLAHGGILVY
                     STCTWSPEENEEVVNWLLQEYDYLELVDIPKLNGMVEGINVPQVARMYPHHFQGEGQF
                     VAKLRDTRSKEAQKIKPKAQKINKMQLQLWQQFAQDHLKIDLNGVLDVFGDQLYLLPN
                     GLPDLSKLKIARNGLHLGTFKKNRFEPSFALGMALSEHDLVQSIEIDIEQFEVYVSGN
                     VVKLDKTVPNGWYQILVKGNGLGFAKVTNNTLKNYYPKGLRFQT"
BASE COUNT         6528 a         3082 c         3359 g         5911 t
ORIGIN      
        1 ttgaacactt tatctttcat tttaaggagt tttatgaaaa agctattaac cactcctgta
       61 atgggttgta ctttggttat actttctcta acgtccctat ttgttggtgt taaatctatc
      121 ccactggagc aaataacaca ccttaatcaa tcacaggtag atattttttt agcgagtcgc
      181 ttaccaagaa ccattagtat cctaatatct ggtgcaagtt tgtcagtctg tggcctgcta
      241 atgcaacaac tcacccaaaa caagttcgtt tcaccgacaa cttcagggac tatggattgg
      301 gcgaaattag gagtagttgt tacgctgatt ttctttaaaa atacctctat ttttattcaa
      361 ctatgtattg cttcaggttt tgccatttta ggatcgctgc tttttgtaac tattttaaaa
      421 atgattactt ttaaggataa tatttttata cctcttatcg gactgatgct tggacaaatt
      481 gtagcagcag caacagtatt tttaggaaca cattttcaag tactacaatc tgtcaacagt
      541 tggcttcaag gtaatttttc catcatgact agccatcgct acgaaattct ttacttagca
      601 ttgccatgtt tgtttttagt gtattttttt gctcatcagt ttaccattgt tgggcttgga
      661 gaaagcttcg ctaaaaattt agggattgct tatgaaaaga tgatttactt tggtttagtt
      721 ttggtttcaa ttatgacatc cctagtcatt attatcgtag gagctttacc tttcttaggt
      781 ttaatcgtac ctaatctaat tagcttaact aaaggggatc atatgtcctc aactatctta
      841 gagacatcgc ttttaggggc ttgtatcgtc atgatatgtg atctttttgg tcgcttagtt
      901 atttttcctt atgaagtctc aataggagtt acgctaggag tattgggtag tgcctttttc
      961 ttgatttcaa ttataagaaa tgagggaaaa tcatgattaa ggatttagag ataccaaaat
     1021 tacttatttt actgatacta ctaattgcta ccattatttt gtttttaatt tatggtattc
     1081 caactgacgc aaatgaattt ttgattatct atatcctaaa aacacgttat caaaaattga
     1141 ttgcattaat tttggttggt atctgcattg gtagttctag tcttattttt caaactctaa
     1201 ccaataaccg acttttaact ccgtccatta ttggtttaga tagcttatat gttcttatcc
     1261 aaacaggatt aatgtattta atcggagctc agcgtgtcat aaaattttca tcttttagca
     1321 gcttcctcct ttcattatta ttaatggttg gattcgctta cttattattt acgatacttt
     1381 ttcgaaataa aaaacagtct ttgaattttg ttctattagc tggactcata tttaatacat
     1441 tattttcttc tatctcatcc tttattcaag ccattatgga ccccaacgat tttatgattt
     1501 tacaaaatca gctctttgct agtttcaatg ctattaatac caagatcctt tggatttctt
     1561 tcattatcat tgtcgttagc tttgtcatta attggccttt tataaaggaa ttggatgtct
     1621 tactattggg taaagaaaat gcgatatcac tcggcatctc atatcaaaag cttactacta
     1681 gaattttttt atggttagca ttgatggtag ctatagcaac agcattagtt gggccgatta
     1741 cttttttagg cttacttgta gcacatatta cctatcacag ttttcatacc tttagacatc
     1801 aaatattagt tcctatagcc attgttatct gtatctttac acttgtctta gggcagcatc
     1861 tcgttcaaaa tctattacac ctcactgttc aactaagtgt tctcctcaac cttatcgggg
     1921 gaagttattt tatattcacc ttaatcaaag gaaggaaaaa cttatgattc aaataaacaa
     1981 tttacataag ttctatggcc aaaaggaaat attaaaagac attaatattt cgattcctaa
     2041 aggaaaagta acagctattt taggtccaaa tggatctgga aagagtacat tgctgagttg
     2101 tatcagtcga cttgaacctt atgataatgg tgaaattttc cttgataaag taccattagc
     2161 ccactacagt tcaaatgact tagccaaaac attagccata ttaagacaat ctaatcactt
     2221 aacactcaag ataaaggtga gtgacttgat aggattcggt agatttcctt atagtaaagg
     2281 acggttaagt caaaaagata aagctgtcat agaatcagtt atatcttata tggatttaaa
     2341 cgatatagct gacgagttca ttaataacct cagcggaggt caaattcaaa gagcttttat
     2401 cgcaatgaca atggcacaag atactcaata tatttgctta gatgagccac tcaacaattt
     2461 agatatgaaa tatgctgttc aaatgatgga tcttatcaaa cgatatgcgt atgagtttaa
     2521 taaaactata gtgattatta ttcacgacat caattttgca actcactacg ctgacaatgt
     2581 tgtcgcttta aaagaaggac aagtggtgtc ttgtgggact gtcgaagatg ttatgcaaga
     2641 aaaaatcctt tctcatttat ttgatatgcc tatccgtatc gaaactgttg atggtaaacc
     2701 aattatcctt ttttgtacaa cgtaaataaa gagatttatg taggataaca tttttagttt
     2761 ttatctattt taggagattt tatttaatga caaaaaaact tattattgct atattagcac
     2821 tatgcactat cttaaccact tctcaagctg ttttagctaa agaaaaatca caaactgtta
     2881 ccataaaaaa caactattcg gtctatatta aaaaagaaaa aagagacaag ccggataata
     2941 aaaagcaaat cagcgagaca cttaaagttc ctttaaaacc caaaaaagta gttgtttttg
     3001 atatgggagc tttggatact atcacagctt taggagctga aaaatctgtt attggtatcc
     3061 cgaaggctaa aaatgctcta agtttattgc ccaataacgt caaatctgtt tataaagcta
     3121 agagatacca agacgtagga agtctcttcg aaccaaactt tgaagctatt gctcgtatgc
     3181 aacctgatgt ggttttccta ggagcacgta tggcttctgt tgataatatt gaaaaattaa
     3241 aggaggctgc acctaaagca gcattagtat atgctggagt cgactcaaaa aaagtatttg
     3301 acaaaggagt tgctgagcgt gtcacaatgt tagggaaaat cttcgaccaa aataaaaagg
     3361 caaaaacctt taataaagat atcgcacaag ctgttcttaa attgcagaaa actattgaga
     3421 aaaaaggtaa acctacagct ctatttgtaa tggcaaacag cggtgaactt ttaactcaat
     3481 caccttctgg tcgttttggt tggattttct ctgtaggtgg atttaaagca gtcaatgaaa
     3541 atgaaaaact aagttcacat ggtactcccg tatcttatga atacatcgct gaaaaaaatc
     3601 ctaactatct ctttgtttta gatcgtggag cgactattgg acaaggagct tcatcaaaag
     3661 aactttttaa taacgatgtt attaaagcaa ctgatgctgt caaaaacaaa cgtgttcatg
     3721 aggtagatgg aaaagattgg tatatcaatt caggcggaag ccgagtaaca ctccgtatga
     3781 ttaaagatgt acagaacttt gttgataatc gttaattttt tgtcaaaggc taggaaaaat
     3841 cctagtctta tttttttgaa aaaatagcta ttatcaatgc ttcagagtat agattgatct
     3901 tatcacttta ttttgtctat tattttcgaa tatagtcaat ggaggtaacc ttatgaatca
     3961 ttttgaacta tttaagctta aaaaagctgg actaacaaat cttaatatca acaatattat
     4021 caactatctc aaaaagaata gtttaacttc tctatctgtt cgcaatatgg ccgtagtatc
     4081 aaaatgtaaa aatcctactt tctttataga aaattataaa cagctagacc ttaaaaaact
     4141 tcgacaagaa ttcaaaaaat ttccagttct atcgattttg gattctaact atcctttaga
     4201 gttaaaagaa atatataatc caccagttct acttttttat cagggtaata ttgaacttct
     4261 atctaaacct aaattagctg tagtgggggc aagacaggca tctcagatag gttgtcagtc
     4321 tgttaaaaag attatcaagg agactaacaa tcaatttgtt atcgtaagtg gtttagcgcg
     4381 tggcattgat acagcagcac atgttagtgc tttaaaaaat ggcggcagta gtatagctgt
     4441 tatcgggagt ggtttagatg tttattatcc aacggagaat aagaaacttc aagaatatat
     4501 gtcatataat catctcgtat tatcagaata ttttaccgga gaacaaccct tgaaatttca
     4561 tttccccgaa cgtaaccgta ttattgcagg gctgtgtcaa ggtattgtgg ttgccgaagc
     4621 taagatgaga tctggaagtt taattacctg tgaaagagca ttagaagagg gacgagaagt
     4681 ttttgccatt cccggaaata ttatcgatgg caaatcagat ggatgccacc atcttatcca
     4741 agaaggagct aaatgcatta tttcaggaaa agatatcctt tctgaatatc agtagtgttt
     4801 tgctttctac tataggagaa gttttctatt gacagtttaa gaaaagtgag ttattatgct
     4861 ataagtttac gatatagaaa gtgataccta tggcaactac gacaaaaacg tcgaccaaaa
     4921 aaacaagtaa aaaaaaatca gctactgcta aaaaaaatct agttattgtg gagtctcctg
     4981 caaaagcaaa gactatcgaa aaatacttag gacgtaacta taaagttgta gcctcagtag
     5041 gtcatatccg tgatttaaaa aagtcaagta tgtccattga ttttgagaat aattacgaac
     5101 cacaatatat taatattcgt ggtaaaggac ctctcattaa tgatttaaaa aaagaagcta
     5161 aaaaggctaa aaaagtttac ctcgcgagtg acccggaccg tgaaggagaa gctatttcct
     5221 ggcatttagc gcatatttta gatttagaca aagaagacag aaaccgtgtt gttttcaatg
     5281 aaatcacaaa agatgctgtt aaaaatgctt ttgtggaacc tcgtcagatt aacatggatc
     5341 tcgttgatgc tcagcaagca cgacgtgttt tagatcgcat tgttggttat tctatctcac
     5401 ctattttatg gaaaaaagta aaaaaagggc tatcagctgg acgtgtacaa tcagttgctc
     5461 ttaaactaat cattgatcgt gaaaatgaga ttaaggcttt ccaaccggag gagtattgga
     5521 ctattgacgg ttcctttaaa aaaggaacac gtaaattcaa tgccactttc tacggtttag
     5581 acggaaagaa attcaaatta tcgaataatg aagacgtcaa aacagttcta aaacgtatta
     5641 aaactgatga attcttagtt gaaaaagttg aaaaaaaaga gcgtcgtcgt aatgcaccat
     5701 taccgtatac aacttcttca ttgcaacaag atgcagctaa taaaatcaat tttcgaactc
     5761 gtaaaaccat gatgattgcg caacagctgt acgaaggact tagcttaggc acagcaggtc
     5821 atcaaggtct gattacctat atgcgtactg attctacacg tattagtccg ttagcacaaa
     5881 atgaagcaac agaatttatt actaaccgtt ttggtgcaaa ttattctaaa catgggaata
     5941 aagttaaaaa tgcttctgga gcacaagacg ctcacgaagc tattcgccca tctagtgtta
     6001 atcatacacc cgaaagtatt gctaagtatt tagacaaaga tcaactaaaa ctttacactc
     6061 ttatctggaa ccgttttatt gcaagccaaa tgacagcagc agtctttgac acaatgaaag
     6121 ttaatttaac gcaaaatggt gttactttta ttgctaatgg tagccaagtc aagtttgatg
     6181 gttacatggc tgtttataac gatactgaca aaaataaaat gttaccagat atggaggaag
     6241 gagaaagtgt taaaaaggtt aatacgaatc ctgaacaaca cttcactcaa cctcctgcaa
     6301 ggttttcaga agcaagtctc attaaaacac ttgaagaaaa cggtgtaggt cgtccttcaa
     6361 cttatgcccc aacgcttgag actattcaaa aacgttatta tgttaagctt gcagctaaac
     6421 gcttcgaacc aactgaactt ggtgaaattg ttaatagcct tattgtagaa tttttcccag
     6481 atattgttga tgttaccttc actgccgaaa tggaagggaa actagacgaa gttgagattg
     6541 gtaaagagca gtggcaaaag attatcgatg aattttataa accatttgaa aaagaacttg
     6601 caaaagcaga aactgaaatg gagaaaattc aaataaagga tgaacctgct ggatttgatt
     6661 gcgagctatg tggatcacca atggtaataa aactaggacg ttatggaaag ttttatgcgt
     6721 gtagcaattt ccctgaatgt cataacacaa aagctatcac taaggaaata ggtgttattt
     6781 gtcctatctg tcaaaaagga caagttattg agcgaaaaac aaaacgcaat cgtatctttt
     6841 atggctgtga ccgctaccca gaatgtgagt ttacatcttg ggacaaacct attggacgaa
     6901 cttgccccaa atcaaatgat ttcttagtgg agaaaaaagt acgcggtggt ggtaaacaag
     6961 ttgtttgctc aaatgaaaaa tgcgactacc aagaagagaa aattaaataa agtggaatct
     7021 gagtgtttag ctcagatttt taaaaaaatt attattttac ataacttaat ttatgtattt
     7081 atttttatgg gagaattaat aaaaaagtgc tataatatag gtgtattcat agtgtgaggt
     7141 gccgttatgt cctatattga aatgaagcat tcctataagc gttatcaaac aggtgaaacc
     7201 gaaattgttg ctaacaatga tattagtttc agtattgaaa gaggtgagtt ggtggttatt
     7261 cttggtgcat ccggcgctgg caaatctact gttctcaata tactaggagg gatggatagt
     7321 aatagtgaag gtgaagtttt aattgatggt aagaatattg ctaattatac catcagggaa
     7381 ttgactcgtt accgtagata tgatgtcggg tttgtttttc aattctataa cttagtccct
     7441 aatctaacag cactagagaa tgtagaattg gcttcagaaa ttgttccaaa ggcattagat
     7501 gcccaacagg cattagaaaa cgtaggtttg ggacaccgta tcaatcattt tccagcacag
     7561 ctttccggtg gcgaacagca acgcgttgca atagcacgag ctattgccaa aaaacctaaa
     7621 ctgttacttt gtgatgaacc tactggtgct ttagattacc aaactggaaa acaagtacta
     7681 gccatcctgc aaaagatggc gcaatcaaaa gaaacaactg tgatcattgt tactcataat
     7741 actgccttag cgccaattgc aaatcgcgtg atccatatgc atgattcaaa aatcagtgat
     7801 attgtaataa acgagaatcc aagtgatatt cagaacatag aatattagag gttatacaga
     7861 tgggaaagac attttggaaa gatatctatc gatcaattac gacttcaaaa ggtagatttt
     7921 catcaatcct cttacttatg atgttaggct catttgcttt tataggactg aaggtctcag
     7981 cacctaatat gcaaagaaca gcacaaaatt accttgctca tcatcatgtt atggatatta
     8041 ccgttttcaa ttcttgggga cttgacaaac atgatcaaac tgttttagaa agcttaaaag
     8101 gatcacaagt tgaattttct tactttgtag atacaacccc tcaacagaat agtaaatctt
     8161 atcgattgta ttccaatact aaaacgatat caacctttga tcttgttaag ggacgtctcc
     8221 ctttaaataa atcagaaatt gctctctcat ttcaggaacg aaaaaaatat gcaataggtg
     8281 ataaaattaa ttttaaacaa gataaaaaca aactgttttc aaatacaggt cctctaacta
     8341 ttgtaggttt tgttaattct accgagattt ggtctaagac aaaccttggt agttctcaaa
     8401 caggggacgg cgacttagat agttatggcg tattagataa gacagcattt cactctccag
     8461 tatatacaat ggcaagggta acttttaaag atcttaggtt gattaaccct ttctcaataa
     8521 gttacaaaga aaaagtagcg aaataccaag aaaaggtatc gcgaaaatta aatattcaca
     8581 ataaaataag atataccaaa acaaaaaaag agagcctacg taaaatcgat gaggaggaaa
     8641 aaagcctact aaaagctcaa aaacaaatca atcggttaga taatgatagc ctagcgatgc
     8701 cactatctca aagacaagct attcaaatga aaattaaaca agaccgacta tctcttttga
     8761 aacgaacaaa agagcttcta aaattgagac ataacaccca aataatggaa tcgcctcaaa
     8821 ttattgtcta caaccgtact acctttcctg gaggacaagg atataacact tttgactcca
     8881 gtacaaatag cacttctaaa atcagtaatc ttttccccat tattttatat ttagtcgcag
     8941 cattagtaac cttaacaaca atgactaggt tcgtcgagga agaaagaacc aacgcaggca
     9001 tattaaaagc actaggctat agtgaccgcc aagttatctt taaatttatt atttatggtt
     9061 ttatagcagg aacattaggg actactttgg gtatcattgg agggcattat cttttacctc
     9121 gtattatttc tgatatcatt tccaaagacc tgactattcc aaacacacag tatcatcttt
     9181 tcttaaatta cagtttacta gcctttgttt tttcattatt aagcattgtt cttccagttt
     9241 ttgttatcac tcgacgtgag ttgaaagaaa aagcagcatt tttattatta ccaaaaccac
     9301 cagctaaagg atctaaaata gctttagagt atatcaattg gatttggaaa aaactgtcct
     9361 tcacacaaaa agtaactgct agaaatattt ttcgttacaa acaaaggatg attatgacca
     9421 tatttggagt tgctggatca gtagcgctct tattttcagg tctaggaata caatcctcct
     9481 taaagcaaac cgtcaatgaa catttcggtc gtattatgcc ctatgatata ttgctaacat
     9541 ataacacaaa tgcttctccc ccaaaaatac ttgaactact ttcaaaagat tcaaaaatag
     9601 acaaatacca accaatacac cttgaaaatc ttgacgaatc cattcctgga caaattaata
     9661 aacagtcaat ttccctattc ataacagaca aaaaacaatt actacctttt atttatttac
     9721 aagaagcaac aactaataag tcattgcact tgaataataa agggattatt atttctaaaa
     9781 aattagctca attttatcat gttaataccg gtgattttat ccatttatct cattcacaaa
     9841 cacttccttc tagaaaatta aaaataacag gagttgtcaa tgcgaatgtt ggtcactata
     9901 tttttatgac aaaacaatac tatcgaacta tttttaagaa agaagctaaa gataacgctt
     9961 ttttggttaa gttaaccaaa cataaaatcg caaataactt agcagaaaaa cttttagaaa
    10021 ttaatggagt tgagtctctt acgcaaaatg ctcttcagct ggctagtgta gaagccgttg
    10081 tgcgttcttt agatggctct atgactattt tagttgtcgt atctttattg ttagccattg
    10141 taattctcta taatctcact aatattaatt tagcagaacg taagcgggag ctatcaacta
    10201 taaaggtatt aggtttttat aatgaagaag taactttata catttaccgc gaaaccatta
    10261 ttttatcaac catcggtgtg attctaggca ccattagcgg tacttattta caccgtcaaa
    10321 tgatgttgct aattggttca gatcaaatac tttttggtga aaaagtatca ccaactacat
    10381 ttataatacc aataagcgta gtagtcatca ttctaataag tctaggtttt atagttaacc
    10441 atcaattaaa aaaactcaat atgttagatg ccttgaaatc agtagattag tttataaaaa
    10501 agattatctt ttctaatctt ttcgataacg attgacagca agtaatctta attgtataat
    10561 ataacagtat aaagttagat aaaagtgaga tatttatgag aaagtctatt tttgaggaaa
    10621 ctgcaaaagg taaagtatca aggcatgtcc ttttaactat attattggct ttgatattac
    10681 tatttacagc agatttcctt acctatatta ttattgaatt gattatctcg cttgattttt
    10741 ggaaactaga gtgggatagc gatatcgtcc agttaatcat tttactagca acttcaatta
    10801 gtacggtaat gttatttatg tgggtgaaat ttaaagagaa gcgtccaatt agttctctag
    10861 ggctatttaa agaaggagcc ctaacagaat tatctagagg atggttagtc gggagcgttc
    10921 tctttggagc ctgcttaata ttaacaataa tttcaggctc tggagttatc gaaactgttc
    10981 gttttacagc tgataaattg atttggtttc ttatttttgc tgttggatgg caggtacaaa
    11041 gcgcatctgt tgaaatattg tgtcgtggat ggttacttcc ggtgagtgcc acaaagtata
    11101 gtaagatagt ttctgtcagt atcagttcaa tctttttcgg tcttctgcat agcgctaata
    11161 atcatgtaag ccttatctcg attttcaatc tgtgtctctt tggactattt ctatcgctct
    11221 atgttatttt aaaaggtaac atctgggggg cgtgtggtat tcatggcgcc tggaactgtg
    11281 tacaagggag tgtctttgga atcgaagtta gtggagagcc aatgttgtct aactccctag
    11341 tccatgttaa aacctatggt gctgactgga ttagcggtgg aaaatttggt gtggaaggaa
    11401 gcatgattac cagtattgtt cttatcgtag catgttattg gctttatcaa aaatcgaatt
    11461 ttccttacga ctaatagatt taatctaaat aagcttctct tactgaaagc ttatttttta
    11521 ttttagtatt aaaaaactat gttttgtgat ataatatttt aaagacgaaa ggagtctatt
    11581 gtgccacaaa atccaacaag taacccagag aaaaaaactt atttattatc tgaattggta
    11641 gactgcttta aaggtaaagc tgtgccttca aaagcagaag caggtgatat ccgtattatt
    11701 aatttgagtg atatgtctcc tttaggaatt gactaccata acttaagaac attccaagat
    11761 gaacaacgat cgcttttaaa atatctactt caggaaggcg atgttttaat cgcttcaaaa
    11821 ggaactgtca agaaagttgc tatttttgaa gagcaagatt accctgtcgt tgcctctgct
    11881 aatattacaa tactaaggcc tacacaacac attagagggt actacttaaa gctttttttt
    11941 gattcagaag aaggtcaaca ggcacttgag aatgctaaca aaggaaaagc tgtgatgaat
    12001 attagcacaa aggaactgtt aaatattgca ataccaagta tcccgctttt tagacaggat
    12061 tatcttatcc aacgttataa acaggggctc aacgattaca aacgtaagat agcacgtgca
    12121 gagcaggagt gggaacgtat ccaaaatgac atcagacaac aacttagcta gaaccaaata
    12181 caaaacatca taggaagagt tatcatgaac gaaatcaaat gccctcattg tggaacagct
    12241 tttgccatca acgagtctga ataccatcaa ttactagaac aaattcgtgg agatgctttt
    12301 gacaaagaag taagtgaacg gttggaaaaa gaacgtctaa tattagggga gcaagcaaaa
    12361 aatcaattac aggaagttgt tgtagaaaaa gacaaggaga tagctaaact tcagtacaaa
    12421 gtcaaacaat ttcttataga aaaagacaat cttctcaaag acaatgagta ccaactcgct
    12481 gagcaattaa atcaaaaaga catgatgctt cgcgaccttg aaaaccaaat cgatagacta
    12541 cgtttagagc atgaaaatag cttgcaagag gcgctaacaa aagtcgaacg agaaagagat
    12601 gcaatacaaa atcagttgca cattcaagaa aaagaaaaag atttagcttt agcttcagta
    12661 aaaagtgatt atgaagtaca actaaaggca gccaatgaac aagtagaatt ctataaaaac
    12721 ttcaaagctc aacagtctac taaagcagta ggagaaagtt tagaacatta tgctgaaaca
    12781 gaatttaata aagtgcgaca tttggccttt cctaatgctt attttgagaa ggacaataca
    12841 ttatcaagtc gtggctcaaa gggggacttt atctatcgag aaaaggatga aaatgacctt
    12901 gagtttttaa gtatcatgtt tgaaatgaaa aatgagtctg atgatactat caagaagcat
    12961 aaaaatgaag attttttcaa agaattagat aaagatcgtc gtgaaaaatc ttgcgaatac
    13021 gcagttttag taactatgct tgaagcagac aatgactatt ataatactgg aattgttgat
    13081 gttagtcaca aataccctaa aatgtacgtt atacgtccac aattttttat ccaattaatt
    13141 ggtattctaa gaaatgcagc actcaatacc ttaaaatata aacaagagct tgctttgatg
    13201 aaagaacaaa atattgacat cacacatttt gaagaagatt tagatatttt caaaaatgca
    13261 tttgctaaaa attataattc tgcaagcaaa aatttccaga aagcaatcga tgaaatagat
    13321 aaatctatta aacgtatgga agctgttaag gctgctttaa caacgtctga aaatcaacta
    13381 cgtcttgcaa ataataaatt agacgatgtt tctgtcaaga aattaacaag aaaaaatcca
    13441 acaatgaaag caaaattcga tgctctaaaa gactaagagg ttatgattat gaagattaga
    13501 actgctacac tagacgatag tgaaaaatta gtccctttgt atcaagaatt aggatacgca
    13561 atatcattat cagagattca atctatttta aaggttattc ttactcattc agattatggc
    13621 tttttgatag ctgaggataa tggcaaacta ctcgccttcg ttggatatca taaactttac
    13681 ttttttgaaa aaagtgggac ttactatcgt attttagcac tagtagttaa tgaaaagcac
    13741 cgtcgtaaag gcatcgctag tcaacttatt aatcacgtta aacagctagc taaaacagat
    13801 ggaagtgaag tactggcttt aaatagcagt ttaaaagaat atcgtcagga agcttatcat
    13861 ttttatgaaa acctagggtt caagaaggtt agtacaggct tttcatacta tttagattag
    13921 gtataaatcc ttatagcact ataccaaaag agcgtaagtt tttacgctcc tttctttgtg
    13981 ttaccgctta tttactgata taatatataa catgattaca ggaataatta atttaaagaa
    14041 agaagctgga atgacatcac acgatgctgt attcaagctt cgaaagatat tacatacaaa
    14101 aaaaatagga cacggcggaa ctcttgatcc agatgttgtt ggggttttac caattgcggt
    14161 tggaaaagcc actcgtgtta ttgaatatat gacagaaagt ggcaaaattt atgaaggaga
    14221 aatcacttta ggatatgcca caagtacaga agattctagt ggagaggtca tttcaagaac
    14281 tccactaact caatccgatc ttagcgagga tgttgtggat catgcgatga aaagttttac
    14341 cggtccaatt acacaagttc ctcctatgta ttcagcagtt aaagtaaatg gtaaaaaact
    14401 ctatgaatat gctcgttcag gagaagaagt agagcgacct aaacgacaaa taacaatttc
    14461 tgaatttaga cgcacaagcc cattgtactt cgaaaaaggc atctgccgtt ttagtttcta
    14521 tgtctcatgc agtaaaggta cctatgttag gacgttagct gttgatctag gaataaaact
    14581 aggatatgct agccatatgt ctttcctaaa aagaacctca tctgcaggat tatctattac
    14641 acaatcatta acgcttgaag aaattaatga aaaatataag caagaagatt tttcattttt
    14701 acttccaatt gaatatggtg ttttagatct cccaaaagtt aacctaactg aggaagataa
    14761 agttgaaatc tcatacggac gtcgtatctt attagaaaat gaagcagata ccttagcagc
    14821 tttttatgaa aatagagtta tcgctatact agaaaaacgt ggcaatgaat ttaaaccaca
    14881 caaagtattg ttataaagaa agtcaatcat gaaaatccat tacattaatg attataaaga
    14941 tattcaagct aaagaagatt gcgtcttagt tttgggctat tttgatggct tacatctggg
    15001 acataaagcc ctctttgata aagctaaaaa aatcgcgacc gaaaaaaatt tgaaaattgt
    15061 cgtcttaacg tttaatgaga cacctcgtct aaccttcgct cgcttccaac ctgaattatt
    15121 gctacatctt acctctccag aaaaacgttc tgaaaaattt caagagtatg gcgtcgatga
    15181 gttatattta atgaatttta ccagtcattt ttcaaaagta tcttctgacc tttttattaa
    15241 gaaatatatt tatggtctca gagcaaaagc tgctgttgtc ggttttgatt ataagttcgg
    15301 tcataatcgt accagtggtg attatctagc gcgtaatttc aagggaccag tctatattat
    15361 tgatgaaatt tctgaaggtg gtgaaaaaat ctcttctaca cgtattcgtc aattgattac
    15421 agagggtaat gttgagaaag ctaaccaact tttgggttat gagttttcaa cctgtggcat
    15481 ggtagtacat ggagatgcta gaggacgaac tatagggttc ccaactgcta atctagctcc
    15541 tattaatcgc acttacttac ctgcagacgg tgtttatatt tccaatgttc tgattaatgg
    15601 aaaatattat agagcaatga cgagcattgg aaaaaatatc acttttggtg gtacagaact
    15661 tcgtttagaa gctaatatct ttgattttga tggcgacatt tatggagaaa ctattgaaat
    15721 tttctggtta aaaagaatca gagaaatggt aaagtttaat ggtattgacg atcttgtaaa
    15781 acaacttaaa aaagataaag aaattgcttt aaattggaaa aaagatagtc aaactcttta
    15841 attttttgta taatagtctc tagtaaatac aagaggcggt tatatgatta ccttattttt
    15901 atcacccagc tgtactagtt gtcgaaaagc gcgtgcttgg ctaagtaagc atgaggttgc
    15961 ttttgaagag cataacatta ttacaagtcc actcaataaa gaagaactat tacaaattct
    16021 atcgtttaca gaaaatggta cagaagacat tatttccaca cgttcaaaag tctttcaaaa
    16081 acttgctatt gatgtagacg aattatcaac gtcttcatta atggaattaa tttcagaaaa
    16141 tccaagtctt ttacgacgcc caattatttt agacaaaaaa cgtatgcaga ttggttttaa
    16201 tgaggatgaa attcgtgcct tcttaccgcg tgactatcgt aaacaagaac tgaaacaggc
    16261 cactattcgt gctgaaattg agggaaaaca tgattagtag taattatagt tatcctcttg
    16321 atccttcatg gaatacggaa gatattacta aggtacttcg ctttttaaat caagttgaac
    16381 acgcctatga aaatagtgtt aaagttgatg atttgctaga tagctataaa gaatttaaaa
    16441 aatttgttaa atccaaggcg caggaaaaac aaattgaccg tgaattccag agaactagcg
    16501 gttattccac ttatcaagct gttaaagcag cacaacaaca agcgaaagga tttatttcac
    16561 ttggacgcta aatttgattt cgctaaacaa ttagtgtata aagctggtca attcattaaa
    16621 tctgagatgc aaaatacttt tgatgttgag gaaaagtctc gatttgatga tttagtgaca
    16681 agtcttgata aaaaaacaca aaaattatta attcaagaaa ttattcaaca ttacccagat
    16741 gataatatac ttgccgaaga agatgatgtg cgctcaccca ttgcacaagg taatgtttgg
    16801 gttttagacc ctattgatgg aactgttaat tttattgttc aaaaagataa ctttgctgtt
    16861 atgttagctt attacgaaga aggcgttgga caatttggta tcatctacga cgttatggct
    16921 gatattcttt acagcggggg aggacatttt gatgtctatg ctaacgataa aaaaattgtt
    16981 ccttttcaag agtgtccttt agaacgttgc ttacttggag taaattctgc tatgtatgca
    17041 gaaaatgatt gcggtattgc ccatttagct tctgaaacct taggtgttcg tatctatggt
    17101 ggtgctggaa ttagcatggc aaaagtcatg cagggcaaat tattagctta tttttcgtat
    17161 attcaacctt gggattatgc tgcagcaaaa atcatggggg agacattagg atttacctta
    17221 ttaacgcttg acggggaaga acccaactat tcaacaagac aaaaagtaat gttcctacca
    17281 aagtcgaaat taaatcttat ccaatcctac cttaccaaaa aataaactta aaaccttcta
    17341 attaaataga aggttttttt gcacgcccgt gattatgtag tataatagaa acatgaaatt
    17401 accgaatgaa tttattgaaa aatatcagac tatacttaaa gatgaagcag aagctttttt
    17461 tgacagtttt gaacaaaaac ccatatcggc atatcgcacc aatcctctca aagaaaagca
    17521 acttgatttt ccaaatgcca tcccaagtac cccttggggg cattatggga aaatatcagg
    17581 aaaatcaata gaacacacaa ctggccttgt ttattctcaa gaacctgctg ctcagatagt
    17641 ggctcagatt gctgaaccac aagaaggaat gaaagtgcta gatttagcag ctgcacctgg
    17701 tggaaaaaca acacaccttc tatcatattt aaacaatact ggtctattag ttagtaatga
    17761 aatttcaaat aaacgtagta aaattttagt cgaaaatgtc gaacgttttg gtgctagaaa
    17821 tgttatcgtg actaatgaaa gctcccaacg actagccaaa tgttttaatt ctttttttga
    17881 tcttattgtt tttgacggtc cgtgttctgg tgagggaatg ttccgcaaag atcctcaagc
    17941 aatacaatat tggcacaaag attaccccac tgaatgtgca cagttacaaa gagatatttt
    18001 aaaagaagca atcaaaatgc tagctcatgg tggtatactt gtatactcta cttgtacatg
    18061 gtcaccagaa gagaacgaag aagtggttaa ttggctcctt caagagtatg attacttaga
    18121 acttgttgat attcctaaac taaatgggat ggttgaaggg attaatgtac cacaagttgc
    18181 aaggatgtac cctcatcatt ttcaaggtga aggacaattc gttgcaaaac tgagagatac
    18241 tagatctaag gaagcacaga aaattaagcc aaaagcacag aaaataaata aaatgcagtt
    18301 acaattgtgg caacaatttg cacaagacca tttaaagata gacttgaacg gtgttttaga
    18361 tgttttcggc gaccaactct atcttctacc taatggtctc cctgatttgt ctaaattaaa
    18421 aatagcacgc aatggtctcc atctaggcac tttcaagaag aatcgttttg agccatcgtt
    18481 tgctttagga atggcactta gcgaacatga cctagtacag tctattgaaa ttgatataga
    18541 acagtttgag gtgtacgtat ctggaaatgt agtcaaacta gacaagactg ttccaaacgg
    18601 ttggtatcaa attcttgtaa aaggcaatgg attaggtttt gcaaaagtga caaataatac
    18661 tcttaaaaat tattacccta aaggactacg atttcagaca taactgttat tgaatacttt
    18721 taataaaaaa ggaaaaagaa atacttttca aaccctagaa acttatggta ctatgggaca
    18781 gtgatgtgtg tttttactat ttgtaaatca ttaaacactc aatgatttta gtaatatata
    18841 tcaactacta aatatcaaaa tttattcaaa agaataggaa
//
LOCUS       DATROQ010000035        18150 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.035, whole genome shotgun sequence.
ACCESSION   DATROQ010000035 DATROQ010000000
VERSION     DATROQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18150
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.035"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..659
                     /locus_tag="VBC94_001081"
     CDS             <1..659
                     /locus_tag="VBC94_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEO6979357.1"
                     /translation="VLVMGLMKILSDDWTSFIFILALILLALRFYNNDSRHNFLLTTS
                     LLLLFLIFMLNPYIIAAVVFAVLYVLINHFSQVKKKNRYALIQFKNHQLDVKTTRNQW
                     LGTDQHESDFYAFEDINIIRISGTDTIDLTNVIVSGQDNVIIIQKVFGDTKVLVPLDV
                     AVKADISSVYGSVQYFDFEEYDLRNESIKLSQEEEYYLLKRVKLVVNTIAGKVEVSRK
                     "
     gene            656..1675
                     /locus_tag="VBC94_001082"
     CDS             656..1675
                     /locus_tag="VBC94_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6979358.1"
                     /translation="MKKHHYFLAFFYGSVIIFAICFVIIDSLGVNLVHLYQTSRLWLI
                     EQLIFSIFFLSLAVTILLLLTWFLLDDNSKRQINHNLRRILNNQSINVTDDGTEISTN
                     IQRLSKKMNLMTASLQSKENSRILKSQEIVKQERKRIARDLHDTVSQDLFAASMVLSG
                     IAQNVSQLDVDQVGSQLLAVEEMLQHAQNDLRILLLHLRPVELENKTLSEGFKMILKE
                     LTDKSDIEVVYHESILTLPKKIEDNIFRIGQEFISNTLKHSQASRLEVYLNQTENELQ
                     LKMIDNGIGFDMDSVYDLSYGLKNIEDRVEDLAGNLQLLSQPGKGVAMDIRLPLVNQS
                     EDKNG"
     gene            1668..2309
                     /locus_tag="VBC94_001083"
     CDS             1668..2309
                     /locus_tag="VBC94_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6979359.1"
                     /translation="MDKIKIVLVDDHEMVRLGLKSFLNLQADVEVIGEASNGLEGIKK
                     ALELRPDVVVMDLVMPEMDGVEATLALLKDWPEAAILVLTSYLDNEKIYPVIEAGAKG
                     YMLKTSSAAEILNAIRKVARGEQAIENEVDKKIKAHDKCPALHEGLTARERDILNLLA
                     KGYDNQRIADELFISLKTVKTHVSNILGKLNVADRTQAVVYAFQHHLVPQDDE"
     gene            2351..3751
                     /locus_tag="VBC94_001084"
     CDS             2351..3751
                     /locus_tag="VBC94_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEO6979360.1"
                     /translation="MDAKTKYKAKKIKAVFFDIDDTLRVKDTGYMPPSILKVFKALKD
                     KGIVVGIASGRARYGVPKEVQDLNADYCVKLNGAYVKDKDKNIIFHRPIPAEYVEQYK
                     KWADTVGIKYGLAGRHEAVLSDRDDLVNDAIDIVYSDLEVNPDFNKEHDIYQMWTFED
                     KGDSLHLPEPLDEHLRLIRWHDHSSDVVLKGTSKALGVSKVVEHLGLKPENILVFGDE
                     LNDLELFDYAGLAVAMGVSHPEAQKKADFITKKVEEDGILYALEELGLIEKELTFPQV
                     DIENTEGPVAVIKTNHGDMTVKLFPDHAPKTVANFIGLAKQGYYDGIIFHRIIPDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNVRGALSMANAGPNTNGSQFFIVQNTKIP
                     YAKKELERGGWPTPIAELYAGQGGTPHLDRRHSVFGQLVNQSSFEVLDEIAAVETGSQ
                     DKPLEDVVILTIEVKE"
     gene            3753..4109
                     /locus_tag="VBC94_001085"
     CDS             3753..4109
                     /locus_tag="VBC94_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEO6979361.1"
                     /translation="MKIGDKLKGTVTGIRPYGAFVSLEDGRTGLIHISEIKTGYIDNI
                     YDVLSVGDEVYVQVIDVDEFTQKASLSLRTLEEERHHIQHRHRFSNNRLKIGFKPLEE
                     NLPSWVEEGLAYLNNK"
     gene            complement(4155..4931)
                     /locus_tag="VBC94_001086"
     CDS             complement(4155..4931)
                     /locus_tag="VBC94_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEO6979362.1"
                     /translation="MTNEKGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQ
                     KMVPETMRDAITNESVIVGEEKSVDDIIEEVLKDIDFYEESGGGITLSGGEIFAQFEF
                     AKAILKRAKSLGIHTAIETTAYTRHEQFIDLIQYVDFIYTDLKHYNSLKHQEKTMVKN
                     ASIIKNIHYAFANGKTIVLRIPVIPNFNDSLEDAEEFACLFDRLDIRQVQLLPFHQFG
                     QNKYRLLNRQYEMEEIAALHPEDLLDYQAIFSKYNIHCYF"
     gene            5052..5807
                     /locus_tag="VBC94_001087"
     CDS             5052..5807
                     /locus_tag="VBC94_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6979363.1"
                     /translation="MNRLENIISLVSQYQKIDVNTLSELLQVSKVTIRKDLDKLEGKG
                     LLHREHGYAVLNSGDDLNVRLSFNHKTKKEIAALAANMVSDNDTILIESGSTCALLAE
                     NICQTKRNVTILTNSCFIANYLREYDSCQIVLLGGEYQSSSQVTVGPLMKKMISLFHV
                     SLAFVGTDGFDPKLGFMGRDLMRSEVAQEMANAADEVIILTDSSKFNQTALVEQLPLS
                     TVSQVITDKHPNSDIANLFQEAEITIQSLLQVP"
     gene            5926..6909
                     /locus_tag="VBC94_001088"
     CDS             5926..6909
                     /locus_tag="VBC94_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEO6979364.1"
                     /translation="MKLERQKLLAKVAYLYYMEGKSQSEIANELGIYRTTISRMLAKA
                     REEGLVRIEISDFNPEIFQLESYFKSKYHLKDIEIVSSRKDSDTSEIEKDLAHVAAAM
                     IRKKIKENDKVGIAWGRTLSKVVEAMRPHPVSQVSFVPLAGGPSHINARYHVNTLVYE
                     MSRRFQGSCTFINATLVQENANLAKGILTSKYFEGLMDNWEKLDVAIVGVGGKPKSNE
                     QQWLDLLNQDDFQCLDEEAAVGEITCRFFNHSGDSVNQHLAKRTIGITLEQLQKVPNR
                     IAVAHGNYKAAALLAVLKKGYINHLVTDFSTALNILRLDKDTFVDTMYQKS"
     gene            7113..7436
                     /locus_tag="VBC94_001089"
     CDS             7113..7436
                     /locus_tag="VBC94_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEO6979365.1"
                     /translation="MEIIVADQIIMGLILNAGDAKQHIYQALKLAKEGNFAESKIEIE
                     LADSALLEAHNLQTQFLAQEAGGTRTDISALFIHSQDHLMTSITEINLIKEIIDLRQE
                     LHIQK"
     gene            7453..7773
                     /locus_tag="VBC94_001090"
     CDS             7453..7773
                     /locus_tag="VBC94_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6979366.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKIAADKEGIEAHIEAYSQGKIAD
                     YAKDLDVALLGPQVSYTLDKSKSICDEYGVPIAVIPMADYGMLDGVKVLKLALSLLET
                     RKEA"
     gene            7775..9076
                     /locus_tag="VBC94_001091"
     CDS             7775..9076
                     /locus_tag="VBC94_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6979367.1"
                     /translation="MSKFDSQKIITPIMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     LGQLPFKGLNQAIANVFGPEWTEPFMQVYSGTFAIMGLISCFAIAYAYAKNSGVEPLP
                     AGVLSLSSFFILMKSSYIPVKGEAIADAISKVWFGGQGIIGAIIIGLVVGAIYTWFIQ
                     HHIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYLIAKVTTGGTFIEMIYDIIQVP
                     LQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKSLLAANRLTL
                     DNGAHIVTQQFLDSFLILSGSGITFGLVIAMLFAAKSKQYKALGKVAAFPAIFNVNEP
                     IVFGFPIVMNPVMFLPFILVPVLAALIVYGAIAVGFMQPFSGVTLPWSTPAIISGFMV
                     GGWQGALVQIVILAISTAVYFPFFKIQDNITYKNECEMERG"
     gene            9257..11713
                     /locus_tag="VBC94_001092"
     CDS             9257..11713
                     /locus_tag="VBC94_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEO6979368.1"
                     /translation="MTKQLLTQKYTNDVQQNSQKHFGYLTERMYSYRDKVLDKKPFID
                     AERAILVTEAYQKHQEKPNVLKRAYMLQNILEKMTIYIDDETMIVGNQASSDKDAPIF
                     PEYTLEFVVNELDLFEKRDGDVFYITEETKEQIRNIAPFWENNNLRARAGVMLPEEVQ
                     VYMETGFFGMEGKMNSGDAHLAVNYQKLLEEGLIGFEKKARKAKADLDLTKPESIDKY
                     HFYDSILITIEAVKTYAERFAILAKKQAKTANTKRRQELLDIASICERVPYYPAETFA
                     EAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDSIVERLTNLWI
                     KTITINKVRSQAHTFSSAGSPLYQNVTIGGQTRHKEDAVNPLSFLVLKSVAQTHLPQP
                     NLTVRYHANLDKSFMNEAIEVMKLGFGMPAFNNDEIIIPSFIKKGVSEEDAYDYSAIG
                     CVETAVPGKWGYRCTGMSYINFPKVLLITMNNGIDPASGKRFAPSYGHFTQMTSYKEL
                     KEAWDKTLRYLTRMSVIVENAIDISLEREVPDILCSALTDDCIGRGKHLKEGGAVYDY
                     ISGLQVGIANLSDSLAALKKLVFEEKRLTTLEVWQALQSDYAGPRGEEIRQMLINEAP
                     KYGNDDDYADSLVRECYDVYVEEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGT
                     LATPDGRHAGTPLAEGCSPSHNMDKKGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTL
                     AKEEDKQKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAF
                     FNVLSKATQDDIIARTEHAL"
     gene            11723..12391
                     /locus_tag="VBC94_001093"
     CDS             11723..12391
                     /locus_tag="VBC94_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO6979369.1"
                     /translation="MEFLLDTLNLEAIKKWHHILPLAGVTSNPTIAKKEGDIHFFQRI
                     RDVREIIGCEASLHVQVVAKDYQGILDDAAKIRQETDDDIYIKVPVTPDGLAAIKTLK
                     AEGYNITATAIYTSMQGLLAISAGADYLAPYFNRMENLDIDATQVIKELAQAIERTGS
                     SSKILAASFKNASQVTKALSQGAQSITAGPDIFESVFAMPSIAKAVNDFADDWKASQH
                     SEHI"
     gene            12459..13547
                     /locus_tag="VBC94_001094"
     CDS             12459..13547
                     /locus_tag="VBC94_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEO6979370.1"
                     /translation="MKVFASPSRYIQGKDALFQSIEHIKSLGQTPLILCDDVVYNIVG
                     ERFLSYLQDDLLPHRVSFNGEASDNEINRVVAVAKEKNSDLIIGLGGGKTIDSAKAIA
                     DKVNLPVIIAPTVASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVIAQAPK
                     RLLASGIADGLATWVEARAVLQKNGIAMAGGRQTLAGVAIAQACERTLFNDSLQALAA
                     CDVKVVTKALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALSGDIHHLTHGEKVAY
                     GTLTQLFLENRPKEEIDRYINLYQAIGMPTTLAELHLGDATYEELLKVGQQATIEGET
                     IHEMPFKISAEDVAAALLAVDRYVSNHQ"
     gene            complement(13699..14625)
                     /gene="cysK"
                     /locus_tag="VBC94_001095"
     CDS             complement(13699..14625)
                     /gene="cysK"
                     /locus_tag="VBC94_001095"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEO6979371.1"
                     /translation="MSKIYNSITDLIGNTPIIQLHHIVPEGAAEVYVKLESFNPGSSV
                     KDRIALAMIEDAEQKGILKAGDTIVEPTSGNTGIGLAWVGKAKGYNVIIVMPETMSIE
                     RRKIIQAYGAQLVLTPGSEGMKGAIAKAKEISAEQNAWLPLQFNNQANPEIHEKTTGR
                     EIIETFGEKGLDAFIAGVGTGGTITGVSRALKKVNPDVAIYAVEADESAILSGEQPGP
                     HKIQGISAGFIPETLATDSYDHIIRVTSNDAIETGRIIGGLEGFLAGISASAAIYAAI
                     EVAKQLGKGKKVLALLPDNGERYLSTSLYDFE"
     gene            complement(14716..15360)
                     /locus_tag="VBC94_001096"
     CDS             complement(14716..15360)
                     /locus_tag="VBC94_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEO6979372.1"
                     /translation="MNYKTIKSDGIVEEEIKKSRFICHLKRVESEEEGRNYITQIKKA
                     HYKANHSCSAMVIGEKGDIKRSSDDGEPSGTAGIPMLTVLEKQGLTNVVAVVTRYFGG
                     IKLGTGGLIRAYSGSVANAIKEIGVVEVKEQIGIRIQLTYPQYQTFDNFLKEHHLQEF
                     ETEFLEAVTCKIYVDPKEFEHTITNLTEFYQGKALLTEEGSQIVEIPFKAETDK"
     gene            15416..16705
                     /locus_tag="VBC94_001097"
     CDS             15416..16705
                     /locus_tag="VBC94_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6979373.1"
                     /translation="MENYLGRLWTKAQLSEQLRKIAINLPSFIKKGSDYICTRCSSSV
                     AKNCQLPTGNYYCRECIVFGRVTSNENLYYFPQKTFSKTNSLKWKGGLTPYQNEVSEE
                     LLKGISSKENLLVHAVTGAGKTEMIYHSIAKVIDTGGSVCIASPRIDVCLELHKRLSN
                     DFRCAITLMHGESPSYQRSPLTIATTHQLLKFYHAFDLLIVDEVDAFPYVDNPILYHG
                     VKQALKKNGTSIFLTATSTTELERKVARKELKKLHLARRFHANPLVIPEMVWVSGIQK
                     SLQTQKLPPKLYQLINKQRQTRYPLLLFFPHIAEGQVFTEMLRQAFPMEKIGFVSSKS
                     TSRLKLVQDFRDNKLSILVSTTILERGVTFPSVDVFVIQANHHLFTKSSLVQISGRVG
                     RALERPEGLLYFLHDGKSKSMHQAIKEIKNMNHIGGF"
     gene            16705..17371
                     /locus_tag="VBC94_001098"
                     /pseudo
     CDS             16705..17371
                     /locus_tag="VBC94_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
     gene            17448..18002
                     /gene="raiA"
                     /locus_tag="VBC94_001099"
     CDS             17448..18002
                     /gene="raiA"
                     /locus_tag="VBC94_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEO6979374.1"
                     /translation="MIKYSIRGENIEVTEAIREYVETKLSKVEKYFNEEQELDTRVNL
                     KVYREKTAKVEVTILIDSITLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKYRE
                     KIPASQVFTTEFEAEPDEEAVSQRIVRTKNVNLKPMDVEEALLQMELLGHDFFIYTDA
                     EDNTTNVLYKREDGELGLIEAKGN"
BASE COUNT         5991 a         2909 c         3535 g         5715 t
ORIGIN      
        1 ttgtcttagt tatgggatta atgaaaattt tatcagatga ctggacaagt tttattttta
       61 ttctcgcttt gattctttta gccttaagat tttataacaa cgatagtcgt cataattttc
      121 tgttaacgac aagtttgtta ttactatttt taatctttat gcttaaccct tacattattg
      181 cagccgttgt ttttgcagtt ttgtatgttc taatcaatca tttctcgcaa gttaagaaaa
      241 aaaatcgtta tgctttgatt caatttaaga accatcaatt agatgttaag acaacacgaa
      301 accaatggtt aggtactgat cagcacgaaa gtgactttta tgcttttgaa gatattaata
      361 ttataagaat ctctgggact gatacaattg atttaacaaa tgttattgta tctgggcagg
      421 ataatgttat tataattcaa aaagtttttg gtgataccaa agtattggtt cctttagacg
      481 ttgccgtaaa ggctgatatt agttctgttt acggtagtgt acagtacttt gattttgagg
      541 agtatgatct aagaaatgaa tctataaaat tgtcacaaga ggaagaatat taccttttga
      601 aacgtgtcaa attagttgta aacactatcg ctgggaaagt tgaggttagt cgtaaatgaa
      661 aaagcaccac tactttttag ctttcttcta tggaagtgtt attatatttg ccatttgttt
      721 tgtcattatt gatagtttgg gtgttaattt ggtgcacctc tatcaaacgt ctcgtttgtg
      781 gctgattgaa caactgattt tctctatttt ctttctatct ttagcggtaa ctatcctttt
      841 attgctaact tggtttttat tagacgataa tagtaaacgt caaattaacc ataatttacg
      901 tcgcatttta aataaccaat caataaatgt taccgatgat ggtactgaaa tatcgaccaa
      961 tattcaacgg ctctctaaaa aaatgaattt aatgacagca agtctacaat ctaaggaaaa
     1021 tagtcgtatt ttaaaaagcc aagaaattgt gaaacaagaa cgtaagcgta ttgcacgtga
     1081 tttacacgac acagttagtc aggatttgtt cgcagcttcc atggttttgt cagggattgc
     1141 acagaatgtt tctcagctag acgttgatca ggttggctcg caattattag cagtagagga
     1201 aatgcttcag catgcacaaa atgatttgag aattctgcta cttcatttga ggccagttga
     1261 attagaaaat aagaccctat ctgaaggatt caaaatgata ttgaaagaat tgaccgataa
     1321 gagcgacatt gaagttgttt atcacgaatc aattctaacc ttacccaaaa agattgaaga
     1381 caatattttt agaattggtc aagaatttat cagtaatact ttaaagcatt cacaagccag
     1441 tcgtctcgaa gtgtacctca atcaaacaga gaatgaattg cagttaaaaa tgattgataa
     1501 tggtattgga tttgatatgg attctgtcta tgatttaagt tatggattaa aaaatattga
     1561 agatcgagta gaagatttag caggaaattt acagcttctc agtcagcctg gtaaaggagt
     1621 tgctatggat ataagattac ctttagtaaa tcagagtgag gataaaaatg gataaaataa
     1681 aaattgtact tgttgatgac catgagatgg ttcgtctcgg tttaaaaagt tttttaaact
     1741 tacaagctga tgttgaagta ataggtgaag cctcaaatgg tttagaaggc ataaaaaaag
     1801 cactggagtt acgtccagac gttgttgtta tggatttagt aatgcctgaa atggacggtg
     1861 tagaagcaac cttagcattg ttgaaggatt ggccagaagc tgctatttta gtcttaacat
     1921 cttacttgga caatgaaaaa atttaccctg ttattgaagc aggtgccaaa ggctatatgt
     1981 taaagacctc aagtgcagca gagattctta atgccattcg taaagttgca aggggagaac
     2041 aagctattga aaatgaagta gataagaaaa ttaaggctca tgataaatgt ccagctttac
     2101 atgagggctt aacagcaagg gaacgtgata tccttaattt attagccaaa ggttatgaca
     2161 atcaaaggat tgctgatgaa ttatttatct cgttaaagac tgttaaaact catgtttcta
     2221 atattttggg aaaactaaat gtggcagatc gaactcaagc ggttgtctat gctttccaac
     2281 accatttggt accacaagat gatgaataag tgatataata gagacactat gttaatgaaa
     2341 gtggttagtc atggacgcaa aaactaaata taaagcaaaa aaaataaagg cggtgttttt
     2401 tgatattgat gacacgcttc gtgttaaaga tacaggttat atgccaccat cgatcttaaa
     2461 agtttttaaa gctttgaaag ataaaggaat tgttgtaggt attgcttcag gaagggcacg
     2521 atatggcgtt ccaaaagaag ttcaagattt aaatgccgat tattgtgtaa aattaaatgg
     2581 agcttatgtt aaggacaagg ataagaatat catcttccat cgtcctattc ctgctgaata
     2641 tgtagagcaa tataagaaat gggcagacac tgttggtatt aagtacggtt tagctggtcg
     2701 tcatgaggca gttttatcag atcgtgatga tttggtaaat gatgcgattg atattgtgta
     2761 ttctgatctc gaagttaatc cagatttcaa taaagaacat gatatttatc agatgtggac
     2821 gtttgaagat aaaggagata gccttcatct acctgaaccg ttagatgaac atcttcgctt
     2881 gatacgttgg catgatcatt cttcagatgt tgtgttaaaa ggaacatcga aggctttagg
     2941 tgtttctaaa gttgttgaac atttgggctt aaagccagag aatatcttgg ttttcggaga
     3001 tgagctcaat gatttagaac tctttgatta tgctggacta gctgtggcga tgggagtgtc
     3061 acacccagaa gctcaaaaaa aagcagattt tatcacaaaa aaagtagaag aagatggcat
     3121 tttgtatgcc ctggaggaac taggtttgat agaaaaagaa ttaacatttc cacaagtaga
     3181 tattgaaaat acagaaggtc ctgtggctgt cattaaaaca aatcacgggg atatgactgt
     3241 aaaactcttc ccggatcatg ctcctaaaac ggtagcaaac tttattggtt tagctaaaca
     3301 aggttattat gatggtatta tcttccatcg tattattcca gattttatga ttcagggtgg
     3361 tgatccaact ggtacaggta tgggtggtga atctatctat ggggaaagtt ttgaagacga
     3421 attttccgaa gaattatata atgttcgcgg agctctttca atggctaatg ctggaccaaa
     3481 tacgaatggc agccaatttt tcatcgtcca aaatacaaaa attccttatg cgaaaaagga
     3541 attagagcga ggtggttggc caacaccaat tgctgagttg tatgctggac aaggtggtac
     3601 accgcatcta gatcgtcgcc acagtgtatt tggacaatta gtaaaccaaa gttcttttga
     3661 ggtacttgat gagattgcgg cagtagagac tggttctcaa gacaaaccat tggaagatgt
     3721 tgttatttta acgattgagg ttaaagaata aaatgaagat tggcgataag ttaaagggaa
     3781 cagtcactgg cattaggcct tatggagcct ttgtttctct tgaggatggt aggactggac
     3841 ttatccatat ttctgaaatt aaaacaggtt acattgataa tatttatgat gttcttagtg
     3901 taggtgatga agtctatgtt caggttatag atgtagatga atttacacaa aaagcaagtt
     3961 tatcgcttcg gacccttgaa gaagaaagac atcatattca gcatcgtcat cgattttcta
     4021 acaatcgtct taagataggt ttcaaaccac ttgaggaaaa tttacctagt tgggttgagg
     4081 aaggtctagc ctacttgaat aataaataaa caaaaaccta gatttgatta atgacaagtc
     4141 taggttttaa atatttaaaa gtagcagtga atattatact tgctaaaaat agcttgataa
     4201 tcgagtagat cctctgggtg tagtgcagcg atctcttcca tttcatattg acgattaagt
     4261 aatcgatatt tgttttgtcc aaattggtgg aaaggtaaga gttgcacttg acggatatct
     4321 aggcgatcaa ataaacaagc aaattcttca gcatcttcta atgaatcgtt aaaattaggt
     4381 atgacaggaa tacgtagcac aatcgtttta ccgttggcaa aagcataatg aatgtttttg
     4441 atgattgaag cattcttaac catcgtcttt tcttggtgtt tgaggctatt gtaatgtttt
     4501 aaatcggtgt aaataaaatc aacgtattgg ataaggtcta tgaattgttc atgtctagta
     4561 taagcagttg tttcaatagc cgtgtggatt cccagtgatt tagcacgttt taaaattgcc
     4621 ttggcaaatt caaattgtgc aaatatttca ccgccagata gcgttatgcc cccaccagat
     4681 tcttcgtaaa aatcaatatc ttttaaaacc tcttcaataa tatcatcaac cgatttttct
     4741 tcgcccacga tgacgctttc attagtaatg gcatctctca ttgtttctgg aaccattttt
     4801 tgcgactcgg gattagcaca ccaaggacat ctaagaggac aaccctttaa gaaaacggtt
     4861 gttcgaatac ctggaccatc gtgaatacta aaatgttgaa tgttgaacac aatacctttt
     4921 tcatttgtca tattattttc ctcaatttca ttttaagtat accttttata cgtaacaaag
     4981 tcaattacga atgaaagttt attgaacttg tttaataaat aaaaaatgat aaaatagaaa
     5041 ggaggtgttt catgaataga ttagaaaata ttattagttt agtatcccag taccaaaaaa
     5101 tagatgttaa tacactctcg gagttgttgc aagtttctaa agtaaccata cgtaaagatt
     5161 tagataaact agaaggcaaa gggcttttgc atagagagca cggttatgca gttttaaaca
     5221 gtggggatga tcttaatgtt aggttatctt ttaaccataa aactaaaaaa gaaattgctg
     5281 ccttagctgc aaatatggtg tctgacaatg acactatctt aatagaatca ggctctactt
     5341 gtgctctttt agcggaaaat atctgtcaga caaagcgaaa tgtcactatt ttgaccaact
     5401 cttgctttat tgctaactat ttacgagagt atgattcatg ccaaattgtc ttacttggtg
     5461 gtgagtatca atcaagttca caggttacgg taggtcctct catgaaaaag atgatttcac
     5521 tttttcatgt ctcgttagct tttgttggga ctgatggttt tgatccaaaa ctaggattta
     5581 tgggtagaga tttaatgcgt tctgaagttg cacaagagat ggcgaatgct gccgatgaag
     5641 tcattatttt aacagattct agtaagttta atcagactgc attggtggaa cagcttccgt
     5701 tgtcaactgt atcacaagtt attacagata aacatccaaa ttcggatatt gctaacttgt
     5761 tccaagaagc agagattacc attcaatctt tattacaagt accataaaag aggctatttt
     5821 tcttacattt tgcacatttg tggcgataat agataaagtg tcatgtcaaa ttgcacagat
     5881 gtaacctttt tgttaaactg aagcaaatgc tgaaaggtta tgagtatgaa attagagcgt
     5941 caaaaattgt tagctaaagt agcttacctt tactatatgg aagggaaaag ccaatctgag
     6001 attgccaatg aattaggaat ttatcgtaca acaataagtc gtatgttagc caaagcgaga
     6061 gaagaaggtc ttgttagaat cgaaatttca gattttaatc cagaaatttt tcagttagaa
     6121 tcctatttta aaagtaagta tcaccttaaa gatattgaga ttgtttcttc gcgaaaagat
     6181 agcgatactt cagagataga gaaggattta gcacatgtag ctgcagctat gattcgaaaa
     6241 aaaattaaag agaatgacaa ggtggggatt gcttggggga ggacacttag taaagtagtt
     6301 gaagcaatgc gtcctcatcc ggtttctcaa gtttcttttg ttcctttggc cggaggtcca
     6361 agtcatatta atgcacgcta tcacgttaat actctggttt acgaaatgtc aaggcgtttc
     6421 caaggaagtt gtactttcat taatgcaact ctagttcaag aaaacgctaa tttagcgaaa
     6481 ggaatattaa cttcaaaata ttttgagggg ctaatggata attgggaaaa acttgatgtg
     6541 gcaattgtgg gcgttggtgg taaacctaaa agcaatgaac aacagtggct tgatctttta
     6601 aatcaagatg attttcaatg cttggatgaa gaagcagctg taggtgaaat tacttgtcgt
     6661 ttttttaatc attcaggtga ttctgtgaat caacatttag ctaaaaggac aataggaatt
     6721 accttagaac aactacagaa agttcctaat agaatagcgg ttgctcatgg caattataag
     6781 gctgcggcac tcttagccgt tttaaaaaag gggtatatta atcatttagt tactgatttt
     6841 tccacagcct taaatatttt aagattagat aaagacacct ttgtggatac tatgtatcaa
     6901 aagagctagg atttattcct agctctttta ataatgtttc tattaccaag tgaattgtta
     6961 tagaataagc acaaaagtgc gctattatca taaagggcac aaggtctatt gacgacgatt
     7021 aagggacatg ttacatttgt tttgcgaaag aaaattagaa ccttttgtaa gaaagtttaa
     7081 acttataaaa aatcattaaa gcggaggtgc ttatggaaat catagtcgca gaccaaatta
     7141 ttatggggct cattttgaat gctggggatg ctaaacaaca tatctatcaa gccttaaaac
     7201 ttgcaaaaga aggaaatttt gcagaaagta aaattgaaat agaactagca gatagtgcac
     7261 tcctagaagc tcataatctt caaacacaat tccttgcaca agaagcagga gggacaagga
     7321 cagatatctc agcactcttt attcattctc aggatcattt gatgacaagt attactgaga
     7381 ttaacttgat taaagaaatt attgatttac gccaggaatt acatattcaa aaataagtaa
     7441 aggggatcaa atatgataaa aattggttta ttctgtgcag caggtttttc aacaggtatg
     7501 ttagtaaaca atatgaaaat cgcagcggat aaagaaggga tagaggcaca tattgaagct
     7561 tattcacaag gaaaaattgc agattatgct aaagatttag atgttgcatt acttggtcca
     7621 caagttagct atactctaga taaatcaaaa tcaatctgtg atgaatacgg tgtaccgata
     7681 gcagttattc caatggcaga ttatggtatg ttggatggtg taaaagtttt gaaacttgct
     7741 ttgagtttat tggaaacaag aaaagaggct tgatatgtca aagtttgata gtcagaaaat
     7801 aattactccg attatgaagt ttgtcaatat gcgagggatt attgcactca aagatggcat
     7861 gctagcaatt ttaccactaa cagttgttgg gagtctcttt ttaatattag ggcagcttcc
     7921 atttaaaggt cttaatcaag ccatagctaa tgtttttgga ccagaatgga cagaaccatt
     7981 tatgcaagtt tattcaggaa cttttgcgat tatgggcttg atttcctgtt ttgcaattgc
     8041 ttatgcttat gctaaaaata gtggtgtaga accgttacct gctggcgttt tatcactttc
     8101 gtctttcttt attttaatga aatcatctta tattcctgta aaaggagaag caatagcgga
     8161 cgctatttct aaggtttggt ttggcggtca ggggattatt ggagcaatta ttattggctt
     8221 agtagttggt gctatctata catggtttat ccaacatcat attgttataa aaatgccaga
     8281 gcaagtacca caggcaatag caaaacaatt tgaagctatg attccagctt ttgttatctt
     8341 tcttttatcg atgattgttt atttgattgc taaggtaaca actggcggta cctttattga
     8401 gatgatttat gatatcattc aagtaccgtt gcaaggctta acaggctcac tttatggagc
     8461 aattgggatt gctttcttta tttcattctt atggtggttt ggtgtccatg gccaatctgt
     8521 ggtgaatggt attgtgactg ctttgttact atccaattta gatgccaata agtctttatt
     8581 agcagcaaat cgcttaacat tagataatgg cgctcacatt gtaacacagc agtttttgga
     8641 tagtttcttg attttatcgg gctcaggaat aacttttgga ctagttattg cgatgctttt
     8701 tgcagcaaaa tcaaaacaat acaaggcact tgggaaagtg gccgcttttc ccgcaatttt
     8761 caatgttaac gagccaatcg tctttggctt tccaatcgta atgaatcctg tgatgtttct
     8821 gccttttatt ttagtaccag ttttggcagc tttaattgtt tatggagcta ttgcggttgg
     8881 ttttatgcaa ccattctcag gtgttacttt accatggagt actccagcaa ttatttctgg
     8941 attcatggta ggtggttggc aaggtgcact cgtccaaata gtcattttag ctatctcaac
     9001 tgctgtttac ttcccattct ttaaaatcca agataatatt acttacaaaa atgaatgtga
     9061 aatggaaagg ggatagctag cagataagag gttgaggtgt gtaatgcacc ttaacctttt
     9121 tctaaaaatg cacatttgtg caactttaga tgtagcattc tattctttat tttcttcttg
     9181 ttattttagg atttaggtgc tattatattt acgaagaaaa actttttagt ttcgaaagaa
     9241 acaagcggag gctgttatga ccaaacaatt actcactcaa aaatatacaa atgatgttca
     9301 acaaaattct caaaagcatt ttggctattt gacagagcgc atgtattctt atcgcgataa
     9361 ggttcttgat aagaaaccat tcatagatgc agaacgtgct atcttggtaa ctgaagccta
     9421 tcaaaagcat caggaaaaac ctaatgtttt aaagagagct tacatgttgc aaaatatttt
     9481 ggaaaaaatg acaatctata ttgacgatga gacaatgatt gtcggtaacc aagcttcatc
     9541 tgataaagat gctcctattt ttccagaata cactctagaa tttgttgtaa atgaactaga
     9601 cctttttgaa aaacgagatg gtgacgtctt ttacattaca gaagaaacca aagaacaaat
     9661 tagaaatatt gcacctttct gggaaaataa taatttacgg gctcgtgcgg gcgttatgct
     9721 tccagaagaa gttcaagttt atatggagac aggcttcttt gggatggaag gtaagatgaa
     9781 ttctggggat gctcacttag ctgttaacta tcagaagtta ctagaagaag ggcttattgg
     9841 ctttgaaaaa aaagcaagaa aagctaaagc tgacttagat ttaacaaagc ctgagagtat
     9901 tgacaaatat catttctatg attctatttt aattacaata gaagctgtta aaacctatgc
     9961 agagcgtttt gctatcctag caaaaaaaca agctaaaaca gcaaatacta agagacgtca
    10021 ggaattgtta gatatcgcta gtatttgtga acgagtgcca tattacccag ctgaaacgtt
    10081 tgcagaagct gttcaatcgg tttggtttat tcagtgtatt cttcaaatag aatcaaacgg
    10141 tcattctcta tcttatggac gctttgatca atacatgtat ccatatgtta aagctgactt
    10201 agaagctgga cgtgagactg aggattcgat cgttgagcgc ctaactaacc tatggattaa
    10261 aacaataact attaataagg ttcgtagcca agctcatacg ttttcgtctg ctggtagtcc
    10321 tctttaccaa aatgtaacga ttggaggaca aacccgacat aaggaagatg ctgttaatcc
    10381 tttatcattt ctagtactaa aatcagtcgc tcagacacac ttaccacagc ctaatctaac
    10441 ggttcgttat catgctaacc tagacaagtc attcatgaac gaagctattg aagttatgaa
    10501 attaggtttt ggtatgccag cttttaataa tgatgaaatt attattccat catttattaa
    10561 aaaaggagtt tccgaagaag atgcctatga ttattcagcg attggttgtg ttgaaacagc
    10621 agttcctggg aaatggggtt atcgttgtac cggcatgagc tatatcaact tccctaaagt
    10681 gttattaatt acaatgaata atggcattga cccggcttct gggaagagat ttgcgccttc
    10741 ttatgggcat ttcacccaga tgacctcata taaagaactt aaagaagctt gggacaaaac
    10801 acttcgttac ttaactcgaa tgagtgttat tgttgaaaat gctattgata ttagtctcga
    10861 aagagaagta cctgatattc tttgttcagc tttaactgat gattgtattg gacgaggtaa
    10921 gcatttaaaa gaaggtggtg cagtatatga ctacatatct ggtctacaag ttggaatcgc
    10981 taatctttca gactcacttg ctgcgcttaa aaaattagta tttgaagaaa aacgcctaac
    11041 cacattagaa gtatggcaag cattgcagag tgattacgca ggtccgcgtg gtgaagaaat
    11101 tcgtcaaatg ttgatcaatg aagcaccaaa atatggaaat gatgatgact acgcggatag
    11161 tttagtacgt gagtgttatg atgtttatgt tgaagaaatt gctaaatatc ccaacacacg
    11221 ctatggacgt ggaccaatcg gtggtattcg ctattcagga acgtcctcta tttcagcaaa
    11281 tgtaggtcaa ggacgaggta ctttagcaac tccagatggt agacatgcag gaactccatt
    11341 agctgaaggt tgttcaccat ctcataatat ggacaaaaag ggacctacct ctgttttgaa
    11401 atcagtttcg aagttaccaa ctgatgagat tgtcggcggt gtactcctta accaaaaagt
    11461 caaccctcaa accttggcaa aagaagaaga taaacaaaaa ttaattgcct tacttagaac
    11521 attcttcaat cgccttcatg gttaccatat ccagtataat gttgtctcac gtgagacttt
    11581 gattgatgct cagaagcacc ctgaaaaaca ccgcgatttg attgtgcgtg tagcaggtta
    11641 ttctgcattc tttaatgtcc tatctaaggc aactcaagat gatattattg cacgtacaga
    11701 acatgctctt taggagatta ttatggaatt tttacttgac actttaaatt tagaagctat
    11761 aaaaaaatgg catcatatac tgccactagc aggtgtaact tcaaatccca ccattgctaa
    11821 gaaagaagga gatatacatt ttttccaacg cattcgtgat gttcgagaaa ttattggatg
    11881 tgaagcaagt ttgcatgttc aagtagtggc caaagattat caagggatac tggatgatgc
    11941 tgctaaaata cgccaagaaa cggatgatga catttatatc aaggttccag ttacgccaga
    12001 tggattagca gcaattaaaa cgttaaaagc agaaggttat aatattacag ccacagcaat
    12061 atacaccagt atgcaaggtc ttttggctat atcagctgga gcagactacc tagcacctta
    12121 ctttaaccga atggagaacc ttgatattga tgcgacacaa gttattaagg aattagcgca
    12181 agcaatagag aggacaggtt cttctagcaa aattttagca gctagtttta aaaacgcttc
    12241 acaagtaacg aaggccttat cacagggtgc tcaatcaatc accgcgggac cagatatttt
    12301 tgaatcagtc tttgcgatgc catctattgc aaaagctgtt aacgactttg cagatgattg
    12361 gaaagccagt caacactcag aacatattta acagagaacg ccaacttttg aggctgtatc
    12421 tttaacataa aattttttaa agaagaaaga ggttgagaat gaaagttttc gcaagtccat
    12481 cgcgctatat acaaggaaag gatgctttat tccaaagtat tgaacatatc aaatctttag
    12541 gtcagactcc acttatcctt tgtgatgatg ttgtttataa tatcgttgga gaaaggtttt
    12601 tatcttatct acaagacgat ttgttaccac atcgtgtctc ctttaacgga gaagcatctg
    12661 ataatgaaat caatcgtgtc gttgctgttg caaaagaaaa gaatagtgac cttattattg
    12721 gacttggggg tggaaaaact attgactctg caaaggcaat tgcagataaa gttaatttac
    12781 cagtgatcat tgcaccaaca gtagcttcaa cagatgcccc aacatcagcg ttatcagtaa
    12841 tctatacaga tgaaggagct tttgaaaaat atatttttta ttcaaagaat ccagacttag
    12901 ttttggtcga tactcaagtc attgctcaag cgccaaaacg tttactagct tctgggattg
    12961 cagatggctt agctacttgg gttgaagcga gagcagtctt acaaaaaaat gggatagcga
    13021 tggctggcgg tagacagact ttagcgggcg tggcaattgc tcaagcatgt gagcgtactt
    13081 tatttaatga tagtttacaa gctttagcgg cttgtgatgt caaagtggtt acaaaagctt
    13141 tagaaaatgt tattgaagct aataccttac ttagtggttt aggttttgaa agcgcaggat
    13201 tagctgcagc tcatgctatt cataatggtt ttactgcctt gagtggagat attcatcact
    13261 taacccatgg tgaaaaagtt gcctatggaa cgttgacaca acttttcctc gaaaatagac
    13321 caaaggaaga aattgatcgc tacattaatc tttaccaagc gattggaatg ccaactacac
    13381 ttgcagaact ccatttgggt gatgcaacat atgaagaact tttaaaagtt ggtcagcaag
    13441 caacaattga aggtgaaaca atacatgaaa tgccattcaa aattagtgca gaggatgtcg
    13501 ctgcagccct gttagcagtt gaccgctatg tttcaaatca tcaataaaaa caatagaacc
    13561 aagtgaaatt gcttggttct atttgtcgtt ttaatatttt aactaaatag ggcttactag
    13621 tatttaaaga gatataaact aaaaaagatc agacaacata tctaatcttt tttagttatc
    13681 gtttactgaa gattaaagtt attcaaaatc atacaggctt gttgataagt aacgttcgcc
    13741 attatcaggg agaagagcta aaaccttttt cccttttcct agttgtttgg ccacttcaat
    13801 agcagcataa atagcagctg aagcagaaat tcctgcgagg aaaccttcta gtccgccgat
    13861 gatacgtcct gtttcaatag catcgttaga tgtaacgcga atgatatgat cataagaatc
    13921 tgtagccaat gtttctggaa tgaaacctgc agaaatccct tgtattttat gaggcccagg
    13981 ttgctcacca gaaaggattg cagattcatc agcttctaca gcataaatag cgacatcagg
    14041 attgactttt tttaatgcac gagagacacc agtaattgtc ccaccagttc ccacacctgc
    14101 gataaaagca tctaaacctt tttcaccaaa agtctcgata atttccctac cagtcgtttt
    14161 ttcgtggatt tctggattgg cttgattatt aaattgcagg ggtagccaag cattttgctc
    14221 tgcagaaatt tcttttgcct tagcaatagc tcccttcatc ccttcactac caggtgttag
    14281 tactaattga gcaccatagg cttgaatgat tttacgacgt tcaattgaca tggtttctgg
    14341 cataacaata ataacattat accctttagc ttttcctacc caagccaaac cgatacctgt
    14401 attaccagat gtaggttcta cgattgtatc tccggcttta agaatccctt tttgctctgc
    14461 atcttcaatc atagctagag cgatacgatc tttgacagat gaacctgggt taaaggattc
    14521 taacttaacg tacacctctg cagctccttc tggaacaata tgatgtaatt gaataatggg
    14581 tgtatttccg attaagtctg taatagaatt ataaattttt gacataagct atacctcttc
    14641 atgacattgg ttaagaatat tataaaactc acattgatta aagtaaaata tcaattcttt
    14701 atcaagtgat agctattatt tatcggtttc agccttaaag ggaatttcaa caatttgact
    14761 cccttcttca gttaacaaag cttttccttg gtagaactct gtcaaattag ttattgtgtg
    14821 ttcaaattcc ttagggtcaa cgtatatttt acaggttaca gcttctaaaa attctgtttc
    14881 aaactcctga aggtggtgct cttttaggaa gttgtcaaaa gtttgatatt gaggataagt
    14941 taattgaata cgaataccaa tttgttcttt aacttctact accccgatct ctttgatggc
    15001 attagctacg ctaccagagt aggcacgaat aagcccccct gtgcctaatt tgatacctcc
    15061 aaaataccgt gtaacgacgg caacaacatt tgtcaggcct tgcttttcaa gtacggttag
    15121 catagggata cctgctgtac cactaggttc accatcatca ctagatcgtt ttatatcccc
    15181 tttttcgcca ataaccatag ctgaacacga atgattggct ttgtagtgcg ctttcttgat
    15241 ttgcgtaata taattacgcc cctcctcttc ggactctact cgctttagat gacaaataaa
    15301 tcgcgatttt ttaatttctt cttcgacaat tccgtctgat tttatcgttt tatagttcat
    15361 agagaaattt tacaaaaatt cttcaaattt agcaaaaaaa tccgaatgaa ataatatgga
    15421 aaattattta ggtagacttt ggacaaaagc acaattatct gagcaactta gaaaaattgc
    15481 aattaacttg ccatctttta taaagaaagg aagcgactat atttgtactc gatgctctag
    15541 ttctgttgcc aaaaattgcc aattaccaac tggaaattat tattgtagag aatgtatagt
    15601 ttttggaaga gttactagta atgaaaattt atattatttc ccacaaaaaa cattttctaa
    15661 aacgaattct ttaaaatgga aagggggact gacaccttat caaaatgagg tttcagaaga
    15721 gttgttgaaa ggtatttcaa gtaaggaaaa tcttcttgtt catgctgtga caggggctgg
    15781 taaaacagaa atgatatacc actctatcgc caaagtaatt gatactggag ggagcgtttg
    15841 tattgccagt cctagaattg atgtttgttt agaattgcat aagcgacttt caaacgattt
    15901 tagatgtgcc ataacattaa tgcatggaga atctccttct tatcagagat cccctttaac
    15961 tattgccaca acacatcaat tgttaaaatt ttatcatgct tttgatttgc taattgttga
    16021 tgaagttgat gcttttccat atgtcgacaa cccgatacta taccatggtg taaaacaggc
    16081 cttaaagaaa aacggaacaa gtatttttct gactgcaact tcaacgactg agttagaaag
    16141 aaaagttgca agaaaagaac taaaaaaatt acatctagct aggagatttc atgccaatcc
    16201 gttagtgata cccgagatgg tttgggtaag tggtatccag aaaagcttgc agacacaaaa
    16261 actacctccc aaattatatc agctgataaa taaacagcga caaacgagat atcctttgtt
    16321 attatttttc ccccatattg cagaaggtca ggtattcacc gaaatgttaa gacaagcctt
    16381 tcctatggaa aaaattggtt ttgtttcaag taagagcaca tctaggttaa agttagtgca
    16441 agattttcga gataacaaat tgtctatcct agtatcaact accattctag agagaggagt
    16501 aactttccct agcgtcgacg tttttgttat acaagcaaat catcatttgt ttacgaaaag
    16561 ttccttggta caaatttcag gaagagttgg aagagcatta gaaagacccg aagggctcct
    16621 ttacttcctt catgatggga aaagtaaatc gatgcaccaa gccataaagg agattaaaaa
    16681 catgaatcat ataggaggat tttaatgact tgtttgttat gtcatgaaat agatttatcg
    16741 caattaactt ttgtggaact tatacttcta aaaccaaagc aaaacgtaat ttgtcaaacg
    16801 tgcaagggta gttttgaagc acttagtagg gagatgggtt gccaaacatg ttgcaagcaa
    16861 atcctccgaa aacaatgtca agattgtatt tattggggta aaaaaggtat agaggtaaat
    16921 catttttccc tttatagata caatgaagca atgaaaaaaa tatttttctc tttttaaatt
    16981 tcaaggggat tatttgttga aagatgtttt tacaaaagaa ataaaggctg ctctaaaaag
    17041 gtataagggc tacactatag tgccagttcc cttgagtcat gaaggatacc aaaataggca
    17101 atttaatcaa gtgattgctt ttctacaatc ggcaaatata ccttataaaa atattctttc
    17161 taaaaaagat ggaggtaagc aatcggctaa taataaagaa gaaagactca aacaagttca
    17221 gcaatttaca ttaaaaaatg aggctgagtt aagggataac cttttaattg ttgatgatat
    17281 ttatacaaca ggcgcaacaa tagcacaaat caggaaacta ttagaagaaa aaggtataaa
    17341 aaatataaaa agtttttcat tagcacgcta aaatcttgca aattttcatg aaagcggtta
    17401 taataagaat atagaaagcg ttaacaacgc aagaaagagg tactcatatg attaaataca
    17461 gtattcgtgg tgaaaacatc gaagtaacag aagctattcg tgagtatgtt gagaccaaac
    17521 tctctaaagt tgaaaaatac tttaatgaag aacaagaatt agatactaga gtcaacctca
    17581 aagtatacag agagaagacc gcaaaagttg aagtaacaat cttaatagat tctattactt
    17641 tacgagcaga agatgtttca caagatatgt atgggtcaat tgacttagtt gttgataaaa
    17701 ttgagcgtca aattcgtaaa aataaaacta aaattgcgaa aaaatatcgt gaaaaaattc
    17761 cagcaagtca agttttcaca acagaatttg aggctgagcc agatgaagaa gcagtaagcc
    17821 aaagaattgt acgtactaag aatgttaatc taaaaccaat ggatgttgag gaagcattgt
    17881 tgcaaatgga acttttaggt catgatttct ttatctatac agatgcagaa gataatacga
    17941 caaatgtcct ttacaagcgt gaagatggcg aactaggttt aattgaagct aaaggaaatt
    18001 aactttgtaa cataatttaa aaaatctcag agttttttct gagatttttt taaaacctat
    18061 tgacaacaaa taaaaaatta tagtatacta aatgagttct taagcgaacc taatattgta
    18121 ttatcatttt ataaacaact taaaaaaagt
//
LOCUS       DATROQ010000036        18138 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.036, whole genome shotgun sequence.
ACCESSION   DATROQ010000036 DATROQ010000000
VERSION     DATROQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18138
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.036"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            114..3347
                     /gene="rexB"
                     /locus_tag="VBC94_001100"
     CDS             114..3347
                     /gene="rexB"
                     /locus_tag="VBC94_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEO6979375.1"
                     /translation="MKLLYTDINHDMTEILVNQAAHAAEAGWRIFYIAPNSLSFEKER
                     AVLETLPQEASFAITITRFAQLARYFTLNQPNQKESLNDIGLAMIFYRALASFEDGQL
                     KVFGRLKQDASFISQLVDLYKELQTANLSILDLKYLHSPEKFEDLLAIFLVVSDLLRE
                     GEYDNQSKIAFFTEQVRSGQLDVDLKNTILIVDGFTRFSAEEEALIKSLSSRCQEIII
                     GAYASQKAYKTNFTNGNIYSAGVDFLRYLATTFQTKPEFILSKWESKSGFEMISKNIE
                     GKHDFTNSSHILDDTAKDCITIWECINQKDEVEHVARAIRQKLYQGYRYKDILVLLGD
                     VDSYKLQLSKIFEQYDIPYYFGKAETMAAHPLVHFMDSLSRIKRYRFRAEDVLNLFKT
                     GIYGEIGQDDLDYFEAYISYADIKGSKKFFTDFVVGAKKFDLGRLNTIRQSLLTPLES
                     FVKTKKQDGIKTLNQFMFFLTQVGLSDNLSRLVGQMSENEQEKHQEVWKTFTDILEQF
                     QTIFGQEKLNLDEFLSLLNSGMMQAEYRMVPATVDVVTVKSYDLVEPHSNQFVYALGM
                     TQSHFPKIAQNKSLISDIERQLINDANDTDGHFDIMTRENLKKNHFAALSLFNAAKQA
                     LVLTIPQLLNESEDQMSPYLVELRDIGVPFNHKGRQSLKEEADNIGNYKALLSRVVDL
                     YRSAIDKEMTKEEQTFWSVAVRYLRRQLTSKGIEIPIITDSLDTVTVSSDVMTRRFPE
                     DHPLKLSSSALTTFYNNQYKYFLQYVLGLEEQDSIHPDMRHHGTYLHRVFEILMKNQG
                     IESFEEKLNSAINKTNQEDVFKSLYSEDAESRYSLEILEDIARATATILRQDSQMTVE
                     SEEERFELMIDNTIKINGIIDRIDRLSDGSLGVVDYKSSAQKFDIQKFYNGLSPQLVT
                     YIDAISRDKEVEQKPPIFGAMYLHMQEPRQDLSKIKNLDDLVTKNHQALTYKGLFSEA
                     EKEFLANGKYHLKDSLYSEAEIAILQAHNQSLYKKASETIKSGKFLINPYTEDAKTVD
                     GDQFKSITGFEADRHMARARALYKLPAKEKRQGFLTLMQQEEENDDL"
     gene            3337..6960
                     /gene="addA"
                     /locus_tag="VBC94_001101"
     CDS             3337..6960
                     /gene="addA"
                     /locus_tag="VBC94_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEO6979376.1"
                     /translation="MTFKPFLNPEDIAVIQTEEKNSDKKQKRTPEQIEAIYTFGNNVL
                     VSASAGSGKTFVMVERILDKLLRGVPIESLFISTFTVKAAGELKERLEKKINESLKSA
                     ESDDLKQFLTQQLVGIQTADIGTMDAFTQKIVNQYGYTLGISPIFRILQDKNEQDVIK
                     NEVYADLFSDYMTGKNAASFIKLVKNFSGNRKDSKAFREMVYKVYAFSQSIDNPKRWM
                     QTVFLKGAQTYTDFEAIPDQEVSSLLNVMQTTANQLRDLTDQEDYKQLTAKGVPTANY
                     KKHLKIIENLVHWSQDFNLLYGKKGLANLARDITNVIPSGNDVTVAGVKYPIFKQLHN
                     RIVGLKHLEVIFKYQGESLFLLELLQSFVLDFSEQYLQEKIQENAFEFSDIAHFAIQI
                     LEENHDIRQIYQDKYHEVMVDEYQDNNHTQERILELLSNGHNRFMVGDIKQSIYRFRQ
                     ADPQIFNDKYKAYQDNPSQGKLIILKENFRSQSEVLDSTNSVFTHLMDEEVGDILYDE
                     SHQLKAGSPRQQERHPNNKTQVLLLDTDEDDIDDSDSQQYDISPAEAKLVAKEIIRLH
                     KEENVPFQDITLLVSSRTRNDGILQTFDRYGIPLVTDGGEQNYLKSVEVMVMLDTLRS
                     IDNPLNDYALVALLRSPMFGFNEDDLTRIAIHDVKMAFYHKVKLSYHKEGHHSDLITP
                     ELSSKIDYFMKTFQTWRDFAKWHSLYDLIWKIYNDRFYYDYVGALPKAEQRQANLYAL
                     ALRANQFEKTGFKGLSRFIRMIDKVLENENDLADVEVALPQNAVNLMTIHKSKGLEFK
                     YVFILNIDKKFSMVDITSPLILSRNQGIGIKYVADMRHELEEEILPAVKVSMETLPYQ
                     LNKRELRLATLSEQMRLLYVAMTRAEKKLYLVGKASQTKWADHYDLVSENNYLPLASR
                     ETFVTFQDWLLAVHETYKKQELFYDINFVSLEELTDHHIGMVNPSLPFNPDNKAENRQ
                     SEDIVHAISVLESVEQINQTYKAAIELPTVRTPSQVKKFYEPILDIEGVDVMETITKT
                     SVDFKLPDFSTSKKQDPAALGSAVHELMQRIEMSSHVKMEDIQKALTEVNAETSIKAA
                     IQIEKINYFFQENSLGKYIQEEVEHLHREAPFAMLKEDPESGEKFVVRGIIDGYLLLE
                     NRIILFDYKTDKFVNPLELKERYQGQMALYAEALKKSYEIERIDKYLILLGGKQLEVV
                     KMD"
     gene            6973..7890
                     /locus_tag="VBC94_001102"
     CDS             6973..7890
                     /locus_tag="VBC94_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6979377.1"
                     /translation="MIVEQKFGNGFTWINIEAEQLRTETSEIQAKYLDSEIITYALDD
                     YERAFMECSHIKGKEVLTIIFNTIDLKQKESYYETVPMTFCLSHDRLITVTRSRNSYM
                     LELLQKYLDRNPDVSPKKFLFAALTLITKQYFNVVSKIDREKDILNRQLREQTTNKRL
                     LAMSDLETGSVYLLTAANQNALVLEQLDVHPSQRFNSEVEKEQLSDALIEAHQLVSMT
                     QLNSQVLSQLSSTFNNVLNNNLNENLTGLNIISINLAIIAAITGFFGMNIPLPLTESR
                     SSWLIVIATSVLLWVIIAQILKRVFNLRK"
     gene            complement(7922..9298)
                     /gene="mnmE"
                     /locus_tag="VBC94_001103"
     CDS             complement(7922..9298)
                     /gene="mnmE"
                     /locus_tag="VBC94_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEO6979378.1"
                     /translation="MSITKEFDTIAAISTPLGEGAIGIVRISGTDALKIASKIYRGKD
                     LSAIQSHTLNYGHIVDPDKNEILDEVMLGVMLAPKTFTREDVIEINTHGGIAVTNEIL
                     QLILRHGARMAEPGEFTKRAFLNGRVDLTQAEAVMDLIRAKTDKAMDIAVKQLDGSLK
                     TLINNTRQEILNTLAQVEVNIDYPEYDDVEEMTTTLMREKTQEFQALMENLLRTARRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREEKAIVTDIEGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRDTDDIVEKIGVERSKKALEEADLVLLVLNSSEPLTLQDRSLLELSKESNRI
                     VLLNKTDLPQKIEVNELPENVIPISVLENENIDKIEERINDIFFDNAGMVEHDATYLS
                     NARHISLIEKAVDSLKAVNEGLELGMPVDLLQVDMTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     gene            9426..11336
                     /locus_tag="VBC94_001104"
     CDS             9426..11336
                     /locus_tag="VBC94_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6979379.1"
                     /translation="MIILQGNKIERSFSGDVLFDNINIQVDQRDRIALVGRNGAGKST
                     LLKILVGEEAPTKGEINKKRDLSLSYLAQDSRFQSENTIFQEMLQVFDSLREVEKRLR
                     ELELQMGQVSGSDLEQLMKTYDILSEEFREKGGFTYESDIKAILNGFKFNSDMWEMPI
                     SELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIDTIAWLENYLVNYQGALIIVSHD
                     RYFLDKVATVTYDLTTHSLDRYVGNYSKFMDLKAEKIATEEKNFEKQQKEIAKLEDFV
                     QRNIVRASTTKRAQARRKQLEKMERLDKPNVEQKSANMTFHAGKVSGNVVLTLENAAI
                     GYEGVSLSEPIDLDVKKFDAIAIVGPNGIGKSTLIKSLVGQIPFIKGEAKLGANVETG
                     YYDQSQSNLTKTNTVLDELWNAFSTTPEVEIRNRLGAFLFSGDDVKKSVSMLSGGERA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIEFDGTLLFVSHDRYFINRVAT
                     KVLEISDKGSTLYLGDYDYYLTKKAELEELARLNEEEVSASKTEIDVTSDYETQKANQ
                     KEFRKITRRVAEIEARLEVLENDENNINGLMLETNDIGKLSDLQKELESIQEEQLLLM
                     EEWENLNMRLDL"
     gene            11486..12454
                     /locus_tag="VBC94_001105"
     CDS             11486..12454
                     /locus_tag="VBC94_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEO6979380.1"
                     /translation="MVTLSKEQHLDMFLKMQRIRDVDMKFNKLVRRGFVQGMTHFSVG
                     EEAASVGAIQDLTDSDIIFSNHRGHGQTIAKGIDIGGMFAELAGKATGTSKGRGGSMH
                     LANLEKGNYGTNGIVGGGYALAVGAALTQQYEGTDNIVIAFSGDSATNEGSFHESVNL
                     AAVWNLPVIFFIINNRYGISTDITYSTKIPHLYMRADAYGIPGHYVEDGNDLMAVYEK
                     MHEVINYVRSGNGPAIVEVESYRWFGHSTADAGVYRTKEEVDSWKAKDPVKRYRAYLI
                     ENEIATEEELAAIEAQVIKEVEEGVKFAEESPFPDMSVAFEDVFVD"
     gene            12529..13527
                     /locus_tag="VBC94_001106"
     CDS             12529..13527
                     /locus_tag="VBC94_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEO6979381.1"
                     /translation="MSETKVMALREAINVAMSEEMRKDEKVFLMGEDVGVYGGDFGTS
                     VGMLEEFGAKRVRDTPISEAAIAGSAIGAAQTGLRPIVDLTFMDFVTIAMDAIVNQGA
                     KTNYMFGGGLSTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGLKVVAPGTVNESKAL
                     LKSSILDNNPVIFLEPKALYGKKEEVNMDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVMQAAEEVAEEGINVEVVDPRTLIPLDKELIIDSVKKTGKLILVNDAYKTGGFTGEI
                     ATMVAESEAFDYLDHPIVRLASEDVPVPYSRVLEQGILPDVAKIKDAIYKVVNKGK"
     gene            13654..15042
                     /locus_tag="VBC94_001107"
     CDS             13654..15042
                     /locus_tag="VBC94_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEO6979382.1"
                     /translation="MAVEIIMPKLGVDMQEGEILEWKKQVGDVVNEGDVLLEIMSDKT
                     NMEIEAEDSGVLLKITHGNGDVVPVTETIGCIGAEGEEVTEASSSENTSVEENATQVT
                     SEPEKVEETSEPSVPAATSGEKVRATPAARKLAREMSIDLALVSGTGANGRVHREDVE
                     SFKGAQPRITPLARRIAEDQGVDIAEITGSGIRGKIVKNDVLAAMSPQAAEAPVKTKA
                     TPTTEEKQLPEGVEVIKMSAMRKAISKGMTNSYLTAPSFTLNYDIDMTEMMALRKKLI
                     DPIMAKTGLKVSFTDLIGMAVVKTLMKPEHRYLNASLINDAQEIELHNFVNIGIAVGL
                     DDGLIVPVVHNADQMSLSDFVIASKDVIKKTQEGKLKSAEMSGSTFSITNLGMFGTKT
                     FNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRIVDGMNGAKFMVDL
                     KNLMENPFGLLI"
     gene            15102..16859
                     /gene="lpdA"
                     /locus_tag="VBC94_001108"
     CDS             15102..16859
                     /gene="lpdA"
                     /locus_tag="VBC94_001108"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEO6979383.1"
                     /translation="MAFDVIMPKLGVDMQEGEILEWKKNEGDTVNEGDVLLEIMSDKT
                     NMEIEAEDTGVLLKIVHQAGDVVPVTEVIAYIGEEGEEVGTSSPSADATITAEDGQSV
                     SGPAAPSQETVAAATPKEELAADEYDIVVVGGGPAGYYAAIRGAQLGGKIAIVEKTEF
                     GGTCLNVGCIPTKTYLKNAEILDGLKVAAGRGINLASTNYAIDMDKTVAFKNSVVKTL
                     TGGVRGLLKANKVEIFNGLGQVNPDKSVVIGDKVIKGRNVVLATGSKVSRINIPGIES
                     PLVLTSDDILDLREIPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAMDKE
                     VSLELQKILAKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVADKALLSIGRVPQMN
                     GLENLEPELEMERGRIKVNAYQETSIPGIYAPGDVNGTRMLAHAAYRMGEVAAENALG
                     GNKRKAHLDFTPAAVYTHPEVAMVGMTEEQAREQYGDILVGKNSFTGNGRAIASNEAH
                     GFVKVIAEPKYKEILGVHIIGPAAAELINEASTIMENELTVYDVAQSIHGHPTFSEVM
                     YEAFLDVLGEAIHNPPKRK"
     gene            16957..17946
                     /locus_tag="VBC94_001109"
     CDS             16957..17946
                     /locus_tag="VBC94_001109"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO6979384.1"
                     /translation="MKYIVNTSNDPAYNVALEAYAFQKLTDIDEIFILWINEPAIIIG
                     RHQNTIQEINKEFIDKNGIHVVRRLSGGGAVYHDLNNLNYTIISNNTQEGAFDFQTFS
                     KPVIDTLAKLGVKAEFTGRNDLEINGQKFAGNAQAYYKGRMMHHGCLLFDVDMSVLGQ
                     ALKVSKDKIESKGIKSVRARVTNIVDHLSDKITVQEFSDAILAQVKEEYPEMDEYVLS
                     DAELSEIQAMRDNQFATWDWTYGKAPEYTIERGVRYPAGKITTYANVENSTIKSVKIF
                     GDFFGVKPVDDIEKMLEGVRYDYKDVLAALKTVDTSQYFSRMTPEEITKAIVD"
BASE COUNT         6051 a         2903 c         3588 g         5596 t
ORIGIN      
        1 taactaaata tcttccccta gatttgaagc tatttttggt ataatattaa taaactgtaa
       61 agaataagtt gaagaaggtt tatttctcag ttgacttata agataggaga ttcatgaaat
      121 tactttatac tgatattaat catgatatga cagaaatctt agtcaatcag gccgcgcatg
      181 ctgctgaagc tggatggcgt attttttata ttgctccaaa ctctttatct tttgaaaagg
      241 agcgtgcagt attagagacc cttcctcaag aagcctcttt tgctattaca attactcgct
      301 ttgctcaatt ggcacgttat tttactttaa accagcctaa tcaaaaagaa agtttaaacg
      361 atattggatt agcaatgatc ttttatcgtg ctttagcaag ttttgaagat ggacaattaa
      421 aagtttttgg acgattgaag caagatgctt cctttatcag tcaattggta gacctataca
      481 aagaattgca gacagcaaat ctgagtattt tagatttaaa atatttacat tctcctgaaa
      541 aatttgaaga tctattagct atttttctag tggtttcaga tcttttaaga gaaggagaat
      601 acgacaatca atctaaaatt gcctttttta cagaacaagt aaggtcagga cagctagatg
      661 tggatttaaa gaacactatc cttatagtcg atggttttac ccgtttctca gctgaagaag
      721 aagctttaat taaaagtctg agttcacgtt gtcaagagat tatcatcgga gcttatgcta
      781 gccaaaaagc gtataagact aactttacca atggaaatat ttattctgca ggggttgatt
      841 ttttaagata tctagcaacg acatttcaaa caaaaccaga atttatcttg tctaagtggg
      901 aaagtaagtc tggttttgaa atgatttcga aaaatatcga aggtaaacat gattttacaa
      961 atagtagtca cattttagac gacactgcta aagattgtat aacaatttgg gagtgcatta
     1021 atcaaaaaga tgaagtggaa catgtggcta gagctatacg tcagaaactt tatcagggat
     1081 atcgctacaa agatatttta gttttactag gagatgtaga tagttataaa ctacaattaa
     1141 gtaagatttt tgaacagtat gatattcctt attatttcgg taaagctgaa acaatggctg
     1201 cgcacccttt agttcatttt atggattcct tgtcgcgaat aaaacgttat cgatttagag
     1261 ctgaagatgt tttaaatctt tttaagacag gtatttatgg agagattggt caagatgatt
     1321 tagattattt tgaagcttat atctcatatg cagatattaa aggttctaag aagttcttta
     1381 cagattttgt tgtgggggct aaaaaatttg atttagggcg tcttaacact attcgtcagt
     1441 ccctccttac tccactggaa agttttgtta aaacgaaaaa acaagacggt ataaaaacac
     1501 tcaaccaatt catgtttttc cttactcagg taggtttgag tgacaattta tcgcgattag
     1561 ttggtcaaat gtctgaaaat gaacaagaaa aacatcaaga agtttggaaa acatttacag
     1621 atattttaga gcagtttcaa acaatatttg gtcaagaaaa attaaattta gacgaatttt
     1681 tatcgttatt aaattcaggt atgatgcaag ccgagtatcg tatggtccct gctactgttg
     1741 atgtggttac tgttaagtct tacgacttgg tagaaccaca tagtaatcaa tttgtctatg
     1801 ccctaggcat gacacaatca cattttccaa agattgctca aaacaaatct ttaatatctg
     1861 atattgaacg tcagcttata aatgatgcca atgacactga tggtcatttt gacattatga
     1921 cgcgagaaaa tttgaagaaa aaccattttg ctgctctctc tctttttaat gctgctaaac
     1981 aagcattagt gttgactata ccacaattat tgaatgagtc tgaagatcag atgtcaccat
     2041 atcttgtcga actaagagat ataggtgttc cttttaatca taaaggacgc caatcgttaa
     2101 aagaagaagc tgataatatt gggaattaca aagctttatt atcgcgtgtt gttgacttgt
     2161 atcgctctgc tattgacaaa gagatgacta aagaagaaca aactttttgg tcagttgctg
     2221 ttcgttattt gcgtcgtcaa ttaacttcta aagggatcga gataccaata attacagata
     2281 gtcttgatac tgtgacagtt tcatcagatg tgatgaccag acgcttccca gaagatcacc
     2341 cacttaaatt atcctcatct gctttgacaa ctttttataa taatcaatat aagtattttt
     2401 tacaatatgt gttaggtttg gaagaacaag actcaattca tccagatatg cggcaccacg
     2461 gaacgtatct tcatcgtgtt tttgaaattc tgatgaaaaa ccaagggata gaatcttttg
     2521 aagagaaact caactctgca attaataaaa ccaatcaaga agatgttttc aaatccttgt
     2581 attcagaaga tgcagaaagt cgttattctt tagaaatttt agaagatatc gcgcgtgcaa
     2641 ctgcaaccat attacgacaa gatagtcaga tgactgttga aagtgaagaa gaacgatttg
     2701 aactaatgat tgataatact ataaaaatta acggtattat tgaccgtatc gatcgtttat
     2761 ctgatggttc tttgggtgtc gtggactata aatcaagcgc tcaaaaattc gatatccaaa
     2821 aattttataa cggtctaagt ccacaattag tcacctacat tgatgctatc agtcgtgaca
     2881 aggaagttga acaaaaacca ccgatttttg gagctatgta cttacatatg caagaaccta
     2941 gacaagactt gtctaaaatt aaaaatttag atgatttagt aacaaaaaat catcaagctt
     3001 taacttataa aggattattt tcagaagccg aaaaagaatt cttagcaaat gggaaatacc
     3061 atctcaaaga ttccctttat tcagaggctg aaattgctat tttacaagca cataatcaat
     3121 cgctttacaa aaaagcatct gagactatta agtcaggtaa gtttttgatc aatccatata
     3181 cagaagatgc taaaacagtt gacggtgatc agtttaagag tattacgggg ttcgaggcgg
     3241 ataggcatat ggcacgtgcc agagcgcttt ataaattacc tgctaaggaa aaacgtcaag
     3301 gtttcttaac attaatgcag caagaggagg aaaatgatga cctttaagcc tttcttaaat
     3361 ccagaagata ttgctgttat tcagacagaa gaaaaaaatt ctgataaaaa acaaaagcga
     3421 acgcctgaac aaattgaagc aatttatact tttggtaaca atgtcctggt atcagcttcg
     3481 gctggttctg gaaagacttt tgtaatggtt gagcggattt tagataaact tttaagaggg
     3541 gttccgattg agtctttgtt tatctcaaca tttacagtaa aagcggcagg tgagctcaaa
     3601 gaacgtttag aaaagaaaat aaacgagtct ttgaaatccg cagaatcaga tgatttaaaa
     3661 cagtttctca cccagcaact tgtcggcatt caaacagctg atattggtac tatggatgct
     3721 tttacgcaaa aaatagttaa ccaatatggt tatactttgg gcatatcacc tatatttagg
     3781 attttacaag ataaaaatga gcaagacgtc ataaagaatg aggtatatgc tgatttattt
     3841 tctgactaca tgactgggaa gaatgcagct tcattcataa aattagtcaa aaatttctca
     3901 ggtaatcgta aagatagcaa agcttttcga gaaatggttt ataaagttta tgcttttagc
     3961 cagtccatcg ataaccccaa gcgctggatg caaacagttt ttctaaaagg tgcacaaact
     4021 tacactgatt ttgaagctat tccagatcaa gaagtttcaa gccttttaaa tgtaatgcaa
     4081 acaactgcta atcaattacg tgatttaacg gatcaagaag attataagca attaactgct
     4141 aaaggggttc cgacagcaaa ttacaaaaaa caccttaaaa taattgaaaa tttagtccat
     4201 tggtctcaag actttaattt gctgtatgga aaaaaaggat tggctaattt ggcacgtgat
     4261 atcactaatg tgattccttc aggcaatgat gttacagttg ctggcgttaa atatcctatc
     4321 tttaaacaat tgcataatcg tatcgttgga cttaagcact tagaagttat ttttaagtat
     4381 caaggagaat ctcttttctt gttagaatta ttgcaatcct ttgttttaga tttctcagaa
     4441 caatatttac aggagaagat tcaagaaaat gcttttgaat tttctgatat tgcccatttt
     4501 gcaattcaaa tattggaaga aaatcatgat attaggcaaa tttatcaaga taagtatcat
     4561 gaagtaatgg ttgatgagta tcaggacaat aaccataccc aagagagaat attagaatta
     4621 ttgtctaatg gacataatcg tttcatggtt ggagacataa aacaatcaat ttaccgtttt
     4681 cgtcaggctg atccacaaat ttttaatgat aaatacaaag cttaccaaga caatccaagt
     4741 caagggaaat taattattct gaaagagaat tttcggagtc aatcagaggt tttagacagt
     4801 acaaatagtg tgtttacaca tcttatggat gaagaagtag gagacattct ttatgatgaa
     4861 agtcaccaat taaaagctgg tagtccaaga cagcaagaaa gacatccgaa taataaaaca
     4921 caagttttac tattagatac tgatgaagat gatatagatg acagtgatag tcaacaatat
     4981 gatattagcc cagctgaagc aaagttagtc gcaaaagaaa ttattcgatt gcataaagaa
     5041 gagaatgttc cctttcaaga tatcaccctc ttagtttctt caaggacaag gaatgatggt
     5101 atcttacaaa cttttgaccg ttatggtatt cctttggtaa ctgacggtgg ggagcaaaat
     5161 tacttaaaat cagtcgaagt gatggtaatg cttgatactt tgcgatcaat tgataatccc
     5221 ttaaatgatt atgctctagt tgctcttttg agatctccca tgtttggctt taatgaagat
     5281 gatttaacaa gaattgctat tcatgatgtc aaaatggctt tttatcataa agtgaaattg
     5341 tcttatcata aagaaggtca tcatagtgat ttaattactc cagaattatc ttctaaaata
     5401 gattacttta tgaagacatt ccagacttgg cgtgattttg ctaaatggca ttcgctttat
     5461 gatttaatat ggaaaatata taatgaccgt ttttattatg attatgtagg agctctgcct
     5521 aaagctgagc aacgtcaagc caatctatat gccttggcac ttcgagcaaa tcagtttgaa
     5581 aaaacaggtt tcaagggttt atcacgtttt attcgaatga ttgataaagt tttagaaaat
     5641 gaaaatgatt tggcagatgt tgaggtggca ttaccgcaaa atgctgttaa cctaatgact
     5701 atacacaaat caaagggtct tgagtttaag tatgtattta ttttaaatat tgataagaaa
     5761 ttttcaatgg tagacataac ttcccctctt attttaagcc gaaatcaagg aattggtatt
     5821 aaatatgttg ctgatatgcg acatgaactc gaagaagaga tactgcctgc agtaaaggtt
     5881 agcatggaaa cacttcctta ccaacttaat aaaagagagt tacgcttagc tactttatca
     5941 gagcaaatgc gtttacttta cgtagctatg acacgtgcag agaaaaaatt atatctggtt
     6001 ggtaaggcta gtcaaaccaa atgggctgat cattatgact tagtatctga aaataattat
     6061 ctcccattag ctagtcgtga gacttttgtg acctttcaag attggttact ggctgttcat
     6121 gaaacctata agaaacaaga gttgttttat gacatcaact tcgtatcatt agaggaatta
     6181 acagatcacc acatagggat ggttaatcct agtctacctt tcaatcccga taataaggca
     6241 gagaatcgtc agtctgaaga tatcgtgcac gcgattagcg tgttagaatc tgttgaacag
     6301 attaatcaaa cttataaagc ggctattgag ttaccgacag taagaacacc aagtcaagtc
     6361 aaaaaattct atgagcctat tctagatata gaaggcgttg atgtgatgga aactattact
     6421 aaaacatcag tagattttaa gttacctgat ttttctacaa gtaaaaagca agacccagca
     6481 gctttaggat ctgctgtaca tgaattaatg caacgcatag agatgtcatc tcatgtcaaa
     6541 atggaagata ttcaaaaagc cttgacagaa gtgaatgctg aaacaagtat caaagcggct
     6601 attcagattg aaaaaatcaa ctattttttc caagaaaact cactcggtaa atatatacaa
     6661 gaagaagtag aacatctaca tcgagaggca ccttttgcca tgttaaaaga agatccggag
     6721 agtggtgaga aattcgttgt acgtggtatt attgatgggt atctactatt agaaaatcgg
     6781 ataattctat ttgattataa gactgataaa tttgtcaatc ctctggaatt aaaagaacgc
     6841 tatcaggggc aaatggcttt atatgcagaa gctttaaaaa aatcttacga gatagaaagg
     6901 atagacaaat acttaattct tttaggtggt aaacagctag aagtggtaaa aatggattaa
     6961 agaaaggaaa aaatgatagt cgaacaaaaa tttggaaatg gttttacttg gattaacatt
     7021 gaagcagagc aattgcgaac agaaacatcg gaaattcaag caaaatattt ggatagtgag
     7081 attattacat atgctctgga tgattatgaa cgagccttta tggaatgttc tcatattaaa
     7141 ggaaaagaag tcttgacgat tatttttaat actattgatc taaagcagaa agaaagttat
     7201 tatgagaccg ttccgatgac cttttgctta tcacacgacc gtttgataac ggtgacacgg
     7261 tctcgaaata gttatatgct agaactattg caaaaatatc tagaccgtaa tccagatgtc
     7321 tctcctaaaa aatttttatt tgcagcttta actttgatta caaaacaata ttttaatgtt
     7381 gtatcgaaaa ttgatagaga aaaggatatt ctcaaccgtc aattgagaga acaaacaacc
     7441 aataagcgct tattagccat gagtgaccta gagacaggct ccgtttatct tttgacagct
     7501 gccaatcaaa atgcattggt attagagcaa ttagatgttc atcctagtca gcgatttaat
     7561 tctgaagtgg aaaaagaaca gttatcagat gctctcattg aagcgcatca gttagtcagt
     7621 atgacacaat taaacagtca ggtactttca caattatcat ctacttttaa taatgtctta
     7681 aataataact tgaacgaaaa cttaacaggt ttgaatatca tttcgattaa tttagcgatt
     7741 attgcagcaa ttactggctt ttttgggatg aatattccat tacctcttac agaatcacgg
     7801 agttcatggt taattgttat tgctacttcg gtcttacttt gggttattat tgctcaaatt
     7861 ttaaaacgag tttttaactt aagaaaataa aaatgttcag tgcgtaagca ccgaacattt
     7921 tttatttacc taagcagaat tggctgaaga gctgagttat tagctcatct ggagctgcat
     7981 ccccagtgat ttctccaagt atttcccaag ttctagtcat atcgacttgt aagagatcaa
     8041 caggcattcc taattccaag ccctcattga cagcttttaa actatcaacg gctttttcaa
     8101 ttagtgagat atggcgagca ttcgatagat aagtagcgtc atgttctacc ataccagcat
     8161 tgtcaaagaa aatatcgtta atacgttctt cgattttatc gatgttttca ttttctaaaa
     8221 ccgaaattgg aataacattt tcaggtaatt cgttaacttc tattttttga ggaagatccg
     8281 ttttattgag taatacgata cgattacttt ctttacttag ttctagaagg gagcggtctt
     8341 gtaatgttag tggttcggaa ctattgagaa ccaacaacac aaggtcggct tcttctaaag
     8401 ccttctttga acgttcaaca ccaatttttt caacgatatc atctgtatca cggataccag
     8461 cagtgtcaac tagttttaaa ggaactcctt taatattgac gtattcttca ataacatcac
     8521 gagttgttcc ttcaatatct gtcacaatcg ccttttcttc acgtaagagg ttatttagta
     8581 agctggattt accgacattt ggacgaccaa tgattgctgt tgaaagtcct tctcgaagaa
     8641 tttttccacg tcgggcagtt cgaagtaaat tttccataag tgcttgaaac tcttgtgttt
     8701 tttcacgcat cagtgttgta gtcatctctt caacgtcatc atattcaggg taatcgatat
     8761 taacttcaac ttgggctaga gtatttagaa tttcttgtcg agtgttattg attagtgtct
     8821 ttaaagaacc atctaattgc ttaacggcaa tatccattgc tttatctgtt ttagctctaa
     8881 taagatccat aacagcttca gcctgagtca agtcaactcg accgtttaaa aaagcacgtt
     8941 tagtaaattc tcctggttca gccattcgtg ctccatgtcg taaaattaac tgaagaattt
     9001 cgttagtaac tgcaattcct ccatgcgtat taatttcaat aacatcttct cttgtgaatg
     9061 tttttggagc aagcataacg cctaacataa cttcatccaa gatttcattt ttatctgggt
     9121 ccacaatatg accatagttt aaggtatggg attggatagc ggatagatct tttccacgat
     9181 aaatttttga tgctatcttt aaggcatctg taccagatat acggacaata ccaatagctc
     9241 cctcaccgag aggagttgaa atagctgcga tggtatcaaa ttcttttgta atagacataa
     9301 agtctctctc tttcttggga attactaaca ttcattgtaa cctaaagccc taaaaggtgc
     9361 aatcatttga ttaaaagaga agatttccat aatggtttta tttgaaggta tgctataata
     9421 aaactatgat tatactacaa ggaaacaaaa ttgagcgctc attttcaggt gatgtgcttt
     9481 ttgataatat taatattcaa gttgatcaac gtgatcgtat tgctcttgtt ggacgtaatg
     9541 gtgctggcaa atcaacgctt cttaaaattt tagttggtga agaggctccg acaaaaggag
     9601 aaataaataa aaagcgtgat ttgagtttgt cctacttggc acaagatagt cgttttcaat
     9661 ctgaaaatac aattttccaa gaaatgttac aagtttttga ttcattaaga gaagtagaga
     9721 agcgcttacg tgaattggaa ttacaaatgg gacaagtttc aggaagtgac ctggaacaat
     9781 tgatgaaaac atacgatatt ttgtcagaag agtttcgtga aaaaggtggc tttacctatg
     9841 aatcagatat aaaggctatt ttaaatggct ttaagtttaa cagtgatatg tgggaaatgc
     9901 ctattagtga attgtctggt gggcaaaata cacgattagc gcttgcaaaa atgctacttg
     9961 aaaagccaga attacttgtt ttagatgaac caacgaacca tttagacatt gatacgattg
    10021 cttggttaga aaattatctt gtaaattatc aaggagccct tattattgtc agccatgacc
    10081 gctatttttt agacaaggta gctacagtaa cttatgattt aacgacacat tctctagacc
    10141 gctatgtagg caattattcg aaatttatgg atcttaaggc agagaaaata gcgactgaag
    10201 aaaagaattt tgaaaagcaa caaaaagaaa ttgctaagtt agaagatttt gtacaacgta
    10261 acattgttag ggcttcaacc actaaacgag cacaagctcg tcgtaaacaa ttagaaaaga
    10321 tggaacgact cgacaaaccg aatgtagagc aaaaatcagc taatatgact tttcatgctg
    10381 ggaaagtctc agggaatgtt gttttaacac ttgagaatgc agctattggt tatgaaggtg
    10441 tttctttatc ggagccaatc gatttggacg tgaagaaatt tgatgctatt gcaattgtag
    10501 gtccaaatgg tatcgggaaa tcaactttaa tcaaatcgct tgttgggcaa attcctttta
    10561 tcaaaggaga agcaaaatta ggagcaaatg tcgaaactgg gtattacgat caaagtcaaa
    10621 gtaatttaac aaaaacgaat actgttttag atgagttatg gaatgctttt tcaacaactc
    10681 cagaagttga aattagaaac cgtttaggag cttttctttt ttcaggtgat gatgttaaaa
    10741 aatcagtttc aatgctatcc ggtggtgaac gagcgcgttt gctattggca aaattatcta
    10801 tggaaaataa taatttcctc atattagatg agccaacaaa ccacttggat attgatagta
    10861 aagaagtctt agaaaatgca ctcatcgaat ttgatggcac attacttttt gttagtcatg
    10921 atcgctattt tattaatcgt gtggcaacta aggttctcga aatttctgat aagggaagta
    10981 ctctctatct aggagattat gattattacc tcactaagaa agccgaacta gaggaattag
    11041 cccgtttaaa tgaagaagaa gtgagtgctt caaaaacaga aatcgacgtt acatcagatt
    11101 atgagacaca aaaagcaaat caaaaggaat ttaggaaaat tacccgtcgc gttgcagaaa
    11161 ttgaggcacg tttggaagtt cttgaaaatg atgaaaacaa tatcaatggt ttaatgttag
    11221 aaaccaatga catcggaaaa ctctctgatt tacaaaaaga gctggaatca atccaagaag
    11281 aacaattgct attaatggaa gaatgggaaa atctaaatat gagactagat ttgtgagaaa
    11341 atttcaaaaa caatagtttg tgaaaaaaag tacattttaa attgagatgc cttttacgaa
    11401 aacaaagaaa gcggttcaaa ttcttgcatc ttgtgaaaaa aacttataca atgattaagt
    11461 aataaagatt tttttggagg ttagaatggt aactctatct aaagaacaac acttggatat
    11521 gtttctaaaa atgcaacgta ttcgtgatgt tgatatgaaa tttaacaaat tggttcgtcg
    11581 tggtttcgtc caagggatga ctcacttctc tgttggtgaa gaagctgctt cagtaggtgc
    11641 tatccaagat ttaacagatt cagatatcat tttctcaaac caccgtggac acggacaaac
    11701 tattgcaaaa ggtattgata ttggtggaat gtttgctgaa cttgctggta aagcaacagg
    11761 aacatcaaaa ggtcgtggag gctcaatgca cttagcaaac cttgaaaaag gtaactatgg
    11821 aacaaacggt atcgtcggtg gaggatatgc gcttgcggta ggtgctgccc ttacacagca
    11881 atatgaagga actgataaca ttgttatcgc cttttcgggt gactcagcga ctaacgaagg
    11941 ctcattccat gagtcagtta acttagctgc tgtttggaac ttaccagtta tcttctttat
    12001 catcaacaac cgttatggta tctcaacaga catcacatac tcaactaaaa ttcctcacct
    12061 ttacatgcgt gctgatgcgt acggtattcc agggcattat gttgaagatg gtaatgatct
    12121 tatggctgtt tacgagaaaa tgcatgaagt tatcaattac gtgcgttcag gaaatggacc
    12181 agctattgta gaggttgaat cataccgttg gtttggtcat tcaactgctg atgctggggt
    12241 ataccgtact aaagaagaag ttgattcatg gaaagctaaa gatccagtaa aacgctaccg
    12301 tgcatactta attgaaaatg aaatcgctac tgaagaagag cttgcagcta ttgaagcaca
    12361 agtgattaaa gaagttgaag aaggggttaa attcgctgaa gaaagtccat tccctgatat
    12421 gtctgttgca tttgaagacg tttttgtgga ctaaaataac ctaaagtaga tgagatttat
    12481 ctcgttctat tattcaattg attaaaaaac ggatattcgg aggaaataat gagcgaaact
    12541 aaagttatgg ctttgcgcga agcgattaat gtcgctatga gcgaagaaat gcgcaaagat
    12601 gaaaaagtat ttttaatggg tgaagatgta ggtgtatatg gaggagactt cggtacatct
    12661 gttggtatgt tagaagagtt tggtgctaag cgcgttcgcg acacaccaat ctcagaggct
    12721 gcgattgcag gttcagctat cggtgctgca caaacaggtt tgcgtcctat cgttgatttg
    12781 acattcatgg atttcgtaac aatcgcaatg gatgctatcg ttaaccaagg tgctaaaacg
    12841 aattatatgt ttggtggagg tctatcaact ccagttactt tccgtgtggc atcagggtca
    12901 ggtattggtt cggctgcaca acactcacaa tcattagaag cttggttaac acacattcca
    12961 ggtctaaaag ttgttgcacc tggtactgtt aatgagtcaa aagcactttt gaaatcttct
    13021 attcttgaca ataacccagt tatcttctta gagccaaaag ctctctatgg taagaaagaa
    13081 gaagttaata tggatcctga tttctatatt ccacttggta aaggggatat taagcgtgaa
    13141 ggtactgact taacaattgt ttcttatggc cgtatgcttg agcgtgttat gcaagctgct
    13201 gaagaagttg ctgaggaagg tattaacgtt gaggttgttg atccacgtac acttattccg
    13261 cttgataaag aattaattat cgattctgtt aagaaaactg gtaaattgat cttggttaac
    13321 gatgcttata aaactggtgg tttcactggt gaaattgcaa ctatggttgc tgaaagtgaa
    13381 gcatttgatt accttgatca tcctatcgtg cgtcttgcaa gtgaagatgt tccagtaccg
    13441 tattcacgtg tacttgaaca aggaatctta cctgatgtag caaaaattaa agatgctatc
    13501 tacaaagtag ttaacaaagg taaataattc ataaaacaaa gagggactga ggaatgagtg
    13561 aaaacttagc ctctttcctt gtttataggt tttaatttat atcgttagat gaaacatata
    13621 tttgacgata tagtttagtg tgaaaggaaa actatggctg tcgaaattat tatgcctaaa
    13681 ctcggggttg acatgcaaga aggcgaaatc cttgagtgga aaaaacaggt aggtgatgtt
    13741 gttaatgaag gtgacgtcct acttgaaatt atgtcagata aaacaaatat ggagattgaa
    13801 gcagaagact caggtgtttt actaaaaatc acacatggga atggagatgt tgttcctgta
    13861 actgagacaa ttggctgtat cggtgctgaa ggtgaagaag taacagaagc atcttcttct
    13921 gaaaatacat cagttgaaga aaacgcgaca caagtaactt cagaaccaga aaaagttgaa
    13981 gaaacatcag aaccaagtgt gccagcagct acaagtggtg aaaaagtacg tgccactcct
    14041 gcagctcgta aattagcaag agagatgtct attgaccttg cactagtatc tggaacaggg
    14101 gcaaatggtc gcgttcacag agaagatgtt gaaagcttca agggtgctca accacgtatt
    14161 acaccattgg cacgtcgaat tgctgaagat caaggagttg atatagcaga aatcactggc
    14221 agtggtattc gtggtaaaat tgttaaaaat gatgttttgg ctgctatgtc accgcaagca
    14281 gcagaggctc ctgtaaaaac gaaagctaca ccaacaacag aagaaaaaca attaccagaa
    14341 ggtgttgaag tcattaagat gtctgcaatg cgtaaggcta tttctaaagg tatgactaac
    14401 tcttacctca ctgctccaag ctttacactt aactatgata ttgatatgac tgaaatgatg
    14461 gctcttcgca agaaattaat tgatccaatc atggctaaaa caggcttgaa agtcagcttt
    14521 actgatttga ttggaatggc cgttgttaag acattgatga aaccagagca tcgataccta
    14581 aatgcttcat taattaacga tgcacaagaa attgagcttc ataattttgt taatattgga
    14641 atcgcagttg gtcttgatga tggtttgatt gttcctgttg ttcacaatgc tgatcaaatg
    14701 tcactttctg actttgttat agcatctaaa gatgttatta agaaaactca agaaggtaaa
    14761 ttaaaatcag ctgaaatgtc aggttcaaca ttctcaatta ctaacttagg tatgtttggt
    14821 actaagacgt ttaacccaat tatcaatcaa ccgaactcag caatccttgg agtaggtgct
    14881 actattccga ctccaacagt ggttgatggt gaaattgtag ctcgaccaat catggcaatg
    14941 tgtttaacaa ttgaccaccg tattgttgat ggaatgaatg gtgctaagtt tatggttgat
    15001 cttaagaact taatggaaaa cccatttggt ttacttattt gattccgtag gtcttaattt
    15061 gatataataa gttattcgaa aaacgaaaga ggaaataaaa aatggctttt gatgtaatta
    15121 tgccaaaact tggggttgac atgcaagaag gcgaaatcct tgagtggaaa aaaaatgaag
    15181 gtgacaccgt taatgaaggt gatgtccttc ttgaaatcat gtctgataaa accaatatgg
    15241 aaattgaagc agaagacact ggtgtacttt taaaaattgt acatcaggca ggagatgttg
    15301 ttcctgtcac tgaagtgatt gcctatatcg gagaagaagg tgaagaagta ggtacgtcat
    15361 caccttcggc tgatgcaact atcacagctg aagatggtca atcagtttct ggacctgctg
    15421 ctccttcaca agagacagtt gcagcagcaa ctcctaaaga agaacttgca gctgatgagt
    15481 acgatatcgt agtcgtaggt ggtggccctg caggttatta cgcagcaatc cgcggtgctc
    15541 aacttggagg aaaaattgcc attgttgaga aaactgaatt tggcggtacg tgtttgaatg
    15601 ttggttgtat ccctacaaaa acatatctta aaaatgctga aatcctagat ggtttgaaag
    15661 tagcagctgg acgtggtatc aaccttgctt caactaacta tgcaattgat atggataaga
    15721 ctgttgcctt taaaaattct gtagttaaga cattgactgg tggtgtacgt ggtctcttga
    15781 aagcgaacaa agttgaaatt ttcaatggac ttggacaagt aaaccctgat aaatctgttg
    15841 ttattggcga taaagttatc aaaggtcgta acgtcgtcct tgcgactggt tctaaggtat
    15901 ctcgtattaa tattccaggt attgaatcac cattagtatt aacatcagat gatattcttg
    15961 atttacgtga aattcctaag tcacttgctg ttatgggtgg tggtgtcgta ggtattgagc
    16021 ttggtcttgt atgggcttca tatggtgttg acgtaactgt tattgaaatg gctgatcgca
    16081 tcattccagc aatggataaa gaagtatcgc tagaacttca aaaaatcctt gctaagaagg
    16141 gaatgaagat taaaacatca gttggtgttt ctgaaattgt tgaagccaat aatcaattaa
    16201 cgcttaaatt aaacaacggc gaagaagttg ttgctgataa agctcttctt tcaattggac
    16261 gtgttccaca aatgaatggt cttgaaaatc ttgagcctga acttgagatg gaacgtggac
    16321 gtattaaagt taatgcttat caagaaactt caattccagg tatctatgca ccaggtgatg
    16381 ttaatggaac tcgtatgtta gctcatgctg cataccgtat gggtgaagta gctgctgaaa
    16441 acgcactcgg cggtaataag cgtaaagcac atctagattt cactccagca gctgtttata
    16501 ctcatcctga agtagcaatg gtggggatga cagaagagca agcacgtgaa caatatggtg
    16561 acatattagt tggtaaaaat agctttacag gcaatggtcg tgctatcgct tcaaacgaag
    16621 cacatggttt tgtaaaagta attgctgaac ctaagtataa agagattctt ggtgttcaca
    16681 ttattggtcc agctgcagcg gaattgatta atgaagcttc tacgattatg gaaaatgagt
    16741 tgactgtcta tgatgtagca caatctatcc atggtcaccc aacattctct gaagttatgt
    16801 acgaagcttt cttagatgtt ctcggtgaag ctattcacaa ccctccaaaa cgcaaataat
    16861 tttgtttaat atatttgaga ttgagctttt atagcttcaa tctcttttca cgtatgtatt
    16921 gtctgaatat tataaaatat tagttaggaa tttaaaatga aatatatcgt taacacgtct
    16981 aatgatccgg cttacaatgt tgctttagaa gcttacgcct ttcaaaagtt gacagatatc
    17041 gatgaaatct ttattctttg gattaatgaa ccggctatca ttattggacg ccatcaaaat
    17101 acaattcaag aaattaacaa agagttcatt gataaaaatg gcatccatgt tgtccgtcgt
    17161 ttgtcaggag gcggagcagt ttatcatgac ttgaataatt taaactacac tattatttct
    17221 aataatacac aagaaggtgc ttttgatttc caaacttttt caaaaccagt tattgatact
    17281 cttgctaaat taggtgttaa agcagaattt actggtcgta atgatttaga aatcaatgga
    17341 caaaaatttg caggaaatgc tcaagcttat tacaaaggtc gtatgatgca tcatggttgt
    17401 cttttatttg atgttgatat gtcagtctta gggcaagcac tcaaagtatc taaagacaaa
    17461 attgaatcta aagggataaa atctgtacgc gcacgtgtta caaatattgt tgaccatctt
    17521 tcagataaaa ttacagtaca agaatttagc gatgccattt tagctcaagt gaaggaagaa
    17581 tatccagaga tggatgaata tgttttgagt gatgccgagt tatcagaaat ccaagctatg
    17641 cgcgataatc aatttgctac atgggattgg acgtatggta aagcacctga atacaccatt
    17701 gaacgtggtg tccgctatcc agctggaaaa attacgactt atgctaatgt tgaaaattca
    17761 acgataaaat ctgtcaaaat ctttggtgac ttctttggag tgaaacctgt agatgatatt
    17821 gaaaaaatgt tggaaggtgt ccgctacgat tacaaagatg ttttagcagc gcttaaaaca
    17881 gttgatacaa gtcaatactt ctcacgtatg acacccgaag agattacaaa agctattgta
    17941 gactaaaatc aaaaaaccta gtcaaagtct ttgcctttgg ctaggttttt tgtatggtta
    18001 agtggcctta tagtacgacg ggcatgtcgt acatctgagg tgcaagtcct ctgtgggcac
    18061 ccgctaccgg tgaacccaat agcgactccc aagcttgact atcgtgaggt agcggggaga
    18121 ggaagggata gcgaaatc
//
LOCUS       DATROQ010000037        17997 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.037, whole genome shotgun sequence.
ACCESSION   DATROQ010000037 DATROQ010000000
VERSION     DATROQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17997
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.037"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..668
                     /locus_tag="VBC94_001110"
     CDS             <1..668
                     /locus_tag="VBC94_001110"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEO6979385.1"
                     /translation="IYSTKDENVIRTVYKDQATMLNGARKETIDGKGALNNQISSLIF
                     EKLNMAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVTAGSFSKRFGVEEGRVLETP
                     IVEFYYKNDDLNDPFINDEHMKFLGIVNDEEIAYLKGETRRINELLKDWFAQIGLNLI
                     DFKLEFGFDKDGKIILADEFSPDNCRLWDAEGNHMDKDVFRRDLGSLTDVYQVVLEKL
                     IAL"
     gene            791..4516
                     /locus_tag="VBC94_001111"
     CDS             791..4516
                     /locus_tag="VBC94_001111"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEO6979386.1"
                     /translation="MNKRIFVEKKADFDIKSASLVKELTHNLQLTSLKDLRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDCLLTETEITAELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLLGSDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPSLDFFETYKADDFAAYKAEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQATYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRDGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRPSDVDTFIA
                     ACNKENIDAVVVATITAKPNLVMTWDGETIVDLERRFLDTNGVRVVVDAKVVDKDLTV
                     PEVRTTSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFDQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFETIQAQHKIT
                     AASAVKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADSVKIG
                     QTQAAFTLTVNGNDLAGASLLSVFEGKLEEVYPTEFEQADALEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEAAIADSVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNKKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLVGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQALFASAVKYFTGK"
     gene            4750..6204
                     /locus_tag="VBC94_001112"
     CDS             4750..6204
                     /locus_tag="VBC94_001112"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEO6979387.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNPSFMGKVKE
                     ALSTVKGGFAYLLMTEDKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDSGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEETSFYIQKVK"
     gene            6232..7254
                     /locus_tag="VBC94_001113"
     CDS             6232..7254
                     /locus_tag="VBC94_001113"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEO6979388.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVSRTERAGVMGALGGFG
                     GVFDLSKTGVREPILVSGTDGVGTKLMLAIKYQQHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYIATGKNNPVKLEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQLLDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVQAALPLIKEELVNGIGHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            7421..7969
                     /gene="purN"
                     /locus_tag="VBC94_001114"
     CDS             7421..7969
                     /gene="purN"
                     /locus_tag="VBC94_001114"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEO6979389.1"
                     /translation="MKIAVFASANGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAVVDLLDKHEIDLVCLAGYMKIVGETLLSAYEGRIINIHP
                     AYLPEFPGAHGIKDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPDVLDSLGIKRK"
     gene            7992..8744
                     /locus_tag="VBC94_001115"
     CDS             7992..8744
                     /locus_tag="VBC94_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6979390.1"
                     /translation="MKLVKNLEIVESIFGDWDETIIWSCVQGIMGEVFVDSLDQPKSS
                     LAKLGRKSSFGFLAGQPTLFLLEVCSGEDIILVPQHKGWSDLIESTYGQNAHSFKRYA
                     TKKDTLFERSRLEKFVTQLPNGFELRVIDEKVYNSCLEKEWSQDLVANYATYQYYKKQ
                     GIGYVIYYQGNIIAGASSYSTYKNGIEIEVDTHPDFRRRGLATIVAAQLILTCLDKGI
                     YPSWDAHTRTSLNLSEKLGYEFSHEYIAYEID"
     gene            8764..10311
                     /gene="purH"
                     /locus_tag="VBC94_001116"
     CDS             8764..10311
                     /gene="purH"
                     /locus_tag="VBC94_001116"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEO6979391.1"
                     /translation="MTKRALISVSDKSGIIDFAKELKNLGWDIISTGGTKVALDDAGV
                     ETIAIDDVTGSPEMMDGRVKTLHSNIHGGILARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDVTNDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     SQTTFKTRQRLAAKAFRHTAAYDALIAEYFTAQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAEKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEVTALEFAWKAIKYVKSNGIIITNDHMTLGLGVGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQESIDAANKHGLTMIFT
                     GVRHFRH"
     gene            10504..11403
                     /locus_tag="VBC94_001117"
     CDS             10504..11403
                     /locus_tag="VBC94_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEO6979392.1"
                     /translation="MNKWLVKASSLVVLGGMVLSAGSRVLADTYVRSIDNGRITTGFN
                     GYPGHCGVDYAVPTGTIIRAVADGTVKFAGAGANFSWMTDLAGNCVMIQHADGMHSGY
                     AHMSRVVARTGEKVKQGDIIGYVGATGTATGPHLHFEFLPANPNFQNGFHGRINPTSL
                     IANVATFSGKTQASAPSIKPLQSAPVQNQSSKLKVYRVDELQKVNGVWLVKNNTLTPT
                     GFDWNDNGIPASEIDEVDANGNLTADQVLQKGGYFIFNPKTLKTVEKPIQGTAGLTWA
                     KTRFANGSSVWLRVDNSQELLYK"
     gene            11550..12854
                     /locus_tag="VBC94_001118"
     CDS             11550..12854
                     /locus_tag="VBC94_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO6979393.1"
                     /translation="MKMNKKVLLTSTMAASLLSVASVQAQETDTTWTARTVSEVKADL
                     VKQDNKSSYTVKYGDTLSVISEAMSIDMNVLAKINNIADINLIYPETTLTVTYDQKSH
                     TATSMKIETPATNAAGQTTATVDLKTNQVFVADQKVSLNTISEGMTPEAATTIVSPMK
                     TYSSAPALKSKEVLAQEQAVSQVAANEQVSPAPVKSITSEVPAAKEEVKPTQTSVSQL
                     TTVSPASVAAETPAPVAKVAPVRTVAAPRVASAKVVTPKVETGASPEHVSAPAVPVTT
                     TSPATDSKLQATEVKSVPVAQKAPTATPVAQPASTTNAVAAHPENAGLQPHVAAYKEK
                     VASTYGVNEFSTYRAGDPGDHGKGLAVDFIVGKNQALGNEVAQYSTQNMAANNISYVI
                     WQQKFYSNTNSIYGPANTWNAMPDRGGVTANHYDHVHVSFNK"
     gene            13101..13799
                     /locus_tag="VBC94_001119"
     CDS             13101..13799
                     /locus_tag="VBC94_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEO6979394.1"
                     /translation="MPHLSKEAFKKQIKNGIIVSCQALPGEPLYTESGGVMPLLALAA
                     QEAGAVGIRANSVRDIKEIQEVTNLPIIGIIKREYPPQEPFITATMTEVDQLATLDIA
                     VIALDCTLRERHDGLSVAEFIQKIKRKYPKQLLMADISTFEEGKNAFEAGVDFVGTTL
                     SGYTDYSRQEEGPDIELLNKLCQAGIDVIAEGKIHTPKQANEINHIGVAGIVVGGAIT
                     RPKEIAERFISGLS"
     gene            13846..15162
                     /locus_tag="VBC94_001120"
     CDS             13846..15162
                     /locus_tag="VBC94_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6979395.1"
                     /translation="MSIKKSVIGFCLGAAALSMFACVDSSQSVMAAEKDKVEITWWAF
                     PTFTQEKAKDGVGTYEKKVIKAFEKKNPNIKVKLETIDFTSGPEKITTAIEAGTAPDV
                     LFDAPGRIIQYGKNGKLADLNDLFTDQFIKDVNNKNIIQASKSGDKAYMYPISSAPFY
                     MAFNKKMLKDAGVLKLVKEGWTTSDFEKVLKALKNKGYTPGSFFANGQGGDQGPRAFF
                     ANLYSAPITDKEVTKYTTDTKNSVKSMKKIVEWIKKGYLMNGSQYDGSADIQNFANGQ
                     TAFTILWAPAQPKTQAKLLESSKVDYLEVPFPSEDGKPDLEYLVNGFAVFNNKDENKV
                     KASKKFITFIADDKKWGPKDVIRTGAFPVRTSFGDLYKGDKRMMKISKWTQYYSPYYN
                     TIDGFSEMRTLWFPMVQSVSNGDEKPADALKDFTQKANDTIKKAAK"
     gene            15250..16137
                     /locus_tag="VBC94_001121"
     CDS             15250..16137
                     /locus_tag="VBC94_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6979396.1"
                     /translation="MRTNKLKMRETMIAYAFLAPILLFFLIFVFAPMVMGFVTSFFNY
                     SMTQFTFIGLANYNRMFHDSIFMKSLINTVIIVIGSVPVVVFFSLFVAANTYEKNVFS
                     RSFYRCVFFLPVVTGSVAVTVVWKWIYDPMSGILNYILKSGHVIEQNISWLGDKHWAL
                     LAIIIILLTTSVGQPIILYIAAMGNIDNSLCEAARVDGANEMQVFWQIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIALISFAQFKILGNDVEY"
     gene            16147..16977
                     /locus_tag="VBC94_001122"
     CDS             16147..16977
                     /locus_tag="VBC94_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO6979397.1"
                     /translation="MKKKTFSAYNFLTALILCLLTVLFIFPFYWIMTGAFKSQPDTII
                     IPPQWWPKAPTLENFKALTVQNPALRWLWNSVFISIMTMFLVCCTSSMAGYVLAKKRF
                     YGQKILFSLFIAAMALPKQVVLVPLVRIINFMGIHDTLWAVILPLVGWPFGVFLMKQF
                     SENISTELLESAKIDGCGEIRTFINVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            16990..17433
                     /locus_tag="VBC94_001123"
     CDS             16990..17433
                     /locus_tag="VBC94_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEO6979398.1"
                     /translation="MIYDHLLNLTHYKDINPNLDLAIDYLLSHDLRNLDIGTYHISPE
                     VILMVQSNQLSESFDHIFEYHKKYLDIHYVIEGYEVIKLGKGDKVEVEEYLGDIGFVK
                     CSEETSFDLRDNYIAFFFPEEAHQPNGMGSLGNYVKKGVLKVLMA"
     gene            17453..>17997
                     /locus_tag="VBC94_001124"
     CDS             17453..>17997
                     /locus_tag="VBC94_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEO6979399.1"
                     /translation="MKKANQLIAAIFDLNNPFMQGCNVVFDLALLNLLFMITCLPLVT
                     IGAAKISLYRTLWQKLEGDQTNLLILYIKHLKKEWFQGMLLGLVELSILVVIIFDLTI
                     LHYQIGFIVPFLKIICYAFLLLTVMTSIYLFPMAARYEMSLLDTVKKSFIMACLNLKW
                     TGVLMFLLIMTWFIMVQSSLL"
BASE COUNT         5477 a         3211 c         3929 g         5380 t
ORIGIN      
        1 atatttatag tacaaaagat gaaaatgtga ttagaacggt ctataaagac caagctacta
       61 tgttgaatgg agctcgtaag gagactattg atggcaaggg agcactaaat aatcagattt
      121 catctcttat ttttgagaaa ttaaatatgg ctggtgtggt gacacattat attgagcaaa
      181 tttcaaaaaa cgaacaattg aataaaaaag ttgatattat tccacttgag gtggtattac
      241 ggaatgtgac agcaggctca ttttcaaaac gttttggagt ggaagaagga cgcgttcttg
      301 aaactccaat tgtggaattt tactacaaaa atgatgatct gaatgatcct tttatcaatg
      361 atgagcatat gaagtttttg ggtattgtta atgatgaaga aattgcttat ctgaaaggag
      421 agactcgccg tattaatgaa ctcttgaaag attggtttgc tcagattggg cttaacttga
      481 ttgacttcaa gttggagttc ggatttgaca aggatggcaa aatcatcttg gctgatgagt
      541 tctcacctga caactgccgt ctatgggatg ctgaaggaaa tcatatggac aaggatgttt
      601 ttcgtagaga tcttggtagc ttgaccgatg tctatcaagt tgtacttgag aagttgatag
      661 cattgtaatt agaagaggaa ggaattgaat ccgttcggga aactcagaaa aaagattggg
      721 cttactgaaa tctcaatttc agatatcctc ctatttttca gtcgctccct tgtcggactt
      781 ggtatcttat atgaataaac gtatttttgt tgagaaaaag gctgactttg acattaaatc
      841 ggctagtctt gtgaaagagt tgacgcataa tctacaactg acttctttga aggatttgcg
      901 tattgtgcag gtctatgatg tcttcaattt ggctgaggat ttgctggcgc gtgctgagaa
      961 gcatattttc tctgagcagg tgacagactg tcttttgacg gaaactgaaa tcactgcgga
     1021 gcttgataag gttgccttct ttgccattga ggcgcttcct ggtcaatttg accaacgtgc
     1081 tgctagttcg caagaagctt tgctattgct tggaagtgac agtcaggtta aggtcaatac
     1141 agcccagctt tacttggtca ataaggatat tgcagaagca gagcttgaag ccgttaagaa
     1201 ctatcttttg aatcctgttg attcacgttt caaggacatt accttgccgc ttgaagagca
     1261 ggctttctct gtgtctgata agacaatccc tagtcttgat ttctttgaaa cttataaggc
     1321 tgacgatttt gcggcctata aggcagagca gggcttggct atggaggtcg atgatcttct
     1381 cttcatccaa gattatttca aatcaatcgg gcgtgtgcca actgaaactg agttgaaagt
     1441 tttggatact tactggtcag accactgccg tcacacaacc tttgaaactg agttgaaaaa
     1501 catcgacttt tcagcttcta aattccaaaa acaattgcag gcgacttatg acaaatatat
     1561 cgccatgcgt gatgaacttg gtcgttctga aaagccgcag acacttatgg atatggcgac
     1621 tatctttggt cgttatgaac gtgccaatgg acgactggat gatatggaag tctcagatga
     1681 aatcaatgcc tgctcagttg aaattgaagt agatgttgat ggtgtaaaag agccttggct
     1741 cctcatgttt aagaatgaga ctcacaatca cccaacagaa attgagccat ttggtggcgc
     1801 agcaacttgt atcggtggtg ccattcgtga cccgttgtca ggacgttcat acgtttatca
     1861 ggctatgcgt atttcaggcg caggcgatat tacgactccg attgcggaaa cacgtgctgg
     1921 taaattgccg caacaggtta tttctaaaac tgcggcgcac ggctattctt catatggtaa
     1981 ccagattggg cttgcgacaa cttatgtgcg tgagtacttt caccctggct ttgtagccaa
     2041 acgcatggag cttggagctg tggttggtgc tgcacctaag gaaaatgtgg ttcgtgaaaa
     2101 accagaagca ggcgatgtgg tcatcttgct cggtggtaaa acaggtcgtg acggtgtcgg
     2161 cggtgcgaca ggttcatcta aggttcaaac ggttgaatct gtggaaacag ctggcgcaga
     2221 ggtacaaaaa gggaatgcca tcgaagaacg taagattcaa cgccttttcc gtgatggtaa
     2281 tgtcactcgc ctaatcaaga aatcaaatga cttcggtgca ggtggtgtct gtgttgccat
     2341 cggtgaattg gctgacggtc ttgaaatcga tttggacaag gtaccgctta aataccaagg
     2401 tcttaatggt actgaaattg caatctcaga atcacaagag cgtatgtcag tcgttgttcg
     2461 tcctagtgac gtggatacct tcatcgcagc ctgcaacaag gaaaatatcg atgccgttgt
     2521 ggttgctact attactgcaa aaccaaacct tgtcatgact tgggatggtg aaacaattgt
     2581 tgacttggaa cgccgtttcc ttgataccaa cggtgtccgt gtcgttgttg atgctaaagt
     2641 cgttgacaaa gacttgacag ttccagaggt acgcacaaca tcagcagaga cacttgaagc
     2701 ggatacgctt aaggtcttgt ctgacctcaa ccacgctagt caaaaaggtc tccaaactat
     2761 ctttgactca tctgttggtc gctcaaccgt taaccaccca atcggtggcc gttaccaaat
     2821 cacaccaaca gaaagctctg ttcaaaaatt gccagttcaa cacggtgtca caacaactgc
     2881 atctgttatg gctcaaggtt acaatcctta tattgcagaa tggtcacctt accacggtgc
     2941 tgcctatgct gtcattgaag cgacagctcg cttggtagca acgggtgctg actggtctcg
     3001 tgcacgtttc tcttaccaag agtatttcga gcgtatggat aaacaggcag agcgttttga
     3061 tcagccagta tcagctcttc ttggttctat tgaggctcag attcaacttg gattgccatc
     3121 aatcggcggt aaggactcta tgtctggtac tttcgaagaa ttgacagtac cgccaacctt
     3181 ggtagctttc ggtgtgacaa cagcggacag ccgcaaggtt ctctctcctg agtttaaagc
     3241 ggctggtgag aatatctact acattccagg tcaagctatt tcagaagata ttgattttga
     3301 ccttatcaag gctaacttta gccagttcga gactattcaa gctcaacata agattactgc
     3361 tgcttcagct gttaaatacg gtggtgtcct agaaagtctt gctctcatga cttttggtaa
     3421 ccgtatcggg gcctctgttg aaattgcaga gcttgacagt agcttgacag ctcaactcgg
     3481 aggctttgtc tttacatcag ctgaggaaat tgctgactcg gtgaaaatcg gtcaaactca
     3541 ggcagccttt acacttactg tcaatggaaa tgaccttgct ggggcaagcc ttctatcagt
     3601 cttcgaaggc aaattggaag aggtttaccc aacagagttt gaacaggcag acgctcttga
     3661 agaagttcct gctgtggtat cagatactgt tatcaaggct aaggaaacaa ttgaaaaacc
     3721 agtggtttac attccagtct tccctggtac caactcagaa tatgattcag ctaaggcctt
     3781 tgaacaggtt ggagctagtg tcaacttggt accatttgta accttgaatg aggctgctat
     3841 tgctgactca gttgacacaa tggtcgctaa tattgctaag gcaaatatca tcttctttgc
     3901 tggaggattc tcagcagcgg atgaaccaga tgggtctgct aagtttatcg tcaatatctt
     3961 gcttaacaag aaggttcgcg cagctattga cagcttcatc gaaaaaggtg gccttatcat
     4021 cggtatctgt aatggtttcc aagcccttgt taaatcaggt cttcttccat acggaaactt
     4081 cgaggaagct ggtgagacaa gtccaaccct cttctataac gatgccaacc agcacgttgc
     4141 caagatggtt gagactcgta tcgcaaatac caactcacct tggttggtag gagttgaggt
     4201 cggcgatatt catgccatcc cagtttcaca cggtgaaggt aaatttgttg tcagcgcttc
     4261 tgaatttgca gagctaagag acaatggtca aatctggagc caatatgtgg actttgacgg
     4321 acaaccatct atggattcta aatacaatcc aaatggctct gtcaatgcca tcgaagggat
     4381 taccagcaag aatggtcaaa tcatcggtaa gatggggcac tcagaacgct gggaagacgg
     4441 cctcttccaa aatatccctg gtaacaaaga ccaagccttg tttgctagtg cagtaaaata
     4501 ttttacagga aaataatttt ttcaaatttt ttcataaaac agataaggaa acaagagcga
     4561 agcgagctta actgtatctt tctgtcgtag tggtatttgg gagccgttga ggctaagact
     4621 caatgattag cttgaaaata gcttgataga ttttctattt tgatgttggc attggtgacg
     4681 atagatccaa aattgagaaa atcatcaaag ctacttaaga tgagatacaa aaagaattag
     4741 gaaaaaagaa tgacatacga agtaaaatct ctaaatgaag aatgtggagt ctttggtatc
     4801 tgggggcatc ctcaggcagc tcaagtcact tactttgggc ttcatagcct tcaacatcgc
     4861 ggtcaagaag gtgctggtat tgtttcgaat gataatggga aactctatgg ttatcgaaat
     4921 gttgggctcc tttctgaagt ttttaagaat caatctgaat tagataattt aactgggaat
     4981 gcggctattg gacatgttcg gtatgctact gcaggttctg cagatattcg caatattcag
     5041 ccttttcttt ataaatttca tgacgggcaa tttgctttat gccataatgg taatttgaca
     5101 aatgctattt ctttaaggaa agaattagaa aagcaaggtg caattttcaa tgcctcctca
     5161 gatactgaaa ttttgatgca cttgattcgt cgaagccata acccaagttt tatggggaag
     5221 gtaaaagaag ctttaagcac tgtaaaggga ggcttcgcct atctactgat gacagaagat
     5281 aaattaattg ctgctcttga ccctaatgcc tttcgtcctt tgtcaattgg acaaatgcaa
     5341 aatggtgctt gggttatttc cagtgagacc tgtgcttttg aggtggtagg cgcaaaatgg
     5401 gttagagatg ttgaacctgg cgaagttatc cttatagacg atagtggtat tcaatgtgat
     5461 cgttatactg atgaaacgca actggcaatt tgttcgatgg aatatgtcta ttttgcaagg
     5521 ccggattcaa ctatacatgg tgttaatgtt catacggctc gaaaaaatat gggaaagcgt
     5581 cttgcacaag aatttaaaca ggatgctgat attgtaattg gtgtcccaaa ttcgtcctta
     5641 tcggctgcta tgggctttgc tgaagaatcc ggattaccaa atgagatggg tcttgtaaaa
     5701 aatcagtata cgcagcgaac ctttattcaa ccgacacaag aattaaggga acaaggtgtt
     5761 cgaatgaaac tatcagcggt atctggtgtt gtcaaaggaa agcgcgttgt tatgattgat
     5821 gactcaattg taagaggaac gacttctaga aggattgtcg gattattaag agaagcagga
     5881 gctagtgaag tacatgttgc tatagctagt ccagaattaa agtatccttg tttttatggt
     5941 attgatattc aaacaagacg agaattaatt tcagcaaatc acgctgctga cgaagtgtgt
     6001 gatattattg gcgcagatag tctgacctat ctctcactag atgggttgat tgaatcaatt
     6061 ggactcgaaa caaaagcacc aaatggtggt ttatgcgtag cttactttga tggacattat
     6121 ccaacaccgc tttatgatta tgaagaagaa tacctcagaa gtttagaaga agaaacaagt
     6181 ttttatattc aaaaagtgaa ataagctctg ctaattatat aaggagaaaa catgtctgaa
     6241 aaaaatgctt atgcaaaatc tggtgttgat gttgaagctg gttatgaagt ggttgaaaga
     6301 ataaaaaaac acgtttctcg tacagaacgt gcgggagtca tgggagctct aggtggcttt
     6361 ggtggggtgt ttgacttgag taaaacagga gttagagagc ctattttggt ttcaggaaca
     6421 gacggtgttg gcacaaaact catgcttgcg attaaatatc aacagcatga caccataggc
     6481 caagattgtg ttgccatgtg tgtcaatgat attattgcag caggtgctga gccattatat
     6541 tttttagatt atattgcgac aggaaaaaat aacccagtca aacttgaaga agttgtttct
     6601 ggcgttgcag aaggttgtgt tcaagcagga gcagcactca ttggaggtga gaccgctgaa
     6661 atgcctggga tgtatggcga agatgattat gaccttgctg gctttgctgt tggtgtggct
     6721 gaaaaatctc agctccttga tggttccaaa gtaaaggaag gcgatattct tttgggactt
     6781 gcttcaagtg gtatccactc aaacggttat tcgctggtgc gtcgtgtctt tgctgactat
     6841 actggtaaag agctgcttcc agagcttgaa ggtaaacaac tcaaggatgt ccttcttgag
     6901 ccaactcgta tctatgttca agcagctctg ccattaatca aggaagaact agtcaacggt
     6961 atcggccaca tcacgggtgg tggctttatc gagaatgttc ctcgtatgtt tgcggatgat
     7021 ttggctgcgg aaatcgatga ggataaggtg ccagtacttc cgattttcaa ggcgcttgaa
     7081 aaatatggtg acatcaagca cgaagaaatg tttgaaatct tcaatatggg tgtcggtctt
     7141 atgctagctg ttagtcctga aaatgttaac cgcgtcaaag agctcttgga cgaaccagtt
     7201 tatgaaatcg gtcgtatcat caagaaagca gacgatagtg tggtgattaa ataagtcact
     7261 aaattttata agaaaggagt gtttagttag tttgcctaac tgaacacggg attaaatttc
     7321 tgggaaaaaa gataaaacga cttagacgca agcgtcgttg ttcgttttcc tatttttcta
     7381 gaaatttttc ggcaagccga atcatccctt tgtatcgaat atgaaaatcg ctgtttttgc
     7441 ttctgctaat ggttccaact ttcaggtcat agcagagcag tttccagtta gttttgtctt
     7501 ttcagatcat cgtgatgcct atgttttaga gcgtgctcag aacttagcca ttccaagctt
     7561 cgcttttgaa ctcaaagagt ttgagaataa ggcggcttac gagcaagccg tcgttgattt
     7621 gttggacaaa cacgagattg acttggtctg tctggcgggc tacatgaaga ttgtcggaga
     7681 aaccttgctc tcagcctatg aagggcgtat catcaatatt cacccagcct atctgcctga
     7741 atttccaggt gctcacggta tcaaggatgc ttgggaggca ggtgttgacc agtctggtgt
     7801 gaccatccat tgggtggact ctggtgtgga taccggtcag gtcatccaac aagtgcgcgt
     7861 gccacgccta gcagacgata gcctagaaag ctttgaaacc cgcatccatg aaaccgaata
     7921 tcaactctat ccagatgttt tggatagttt gggcataaag agaaaatagt aaaatttgtt
     7981 ataataagct aatgaaacta gtgaaaaatt tagagatagt agagtctatt tttggtgatt
     8041 gggacgagac cataatctgg tcgtgtgtcc aaggtattat gggagaagtt tttgtagata
     8101 gtttagacca accaaaatct tctttggcaa aattgggaag gaaatcatct tttggctttc
     8161 tagcaggtca gcctacttta tttctactag aagtttgctc tggggaggat attatccttg
     8221 ttcctcagca taaaggatgg tcagatttga ttgaatcaac ttatggccag aatgcccatt
     8281 cttttaagcg ttatgcgacg aaaaaagata ctttgtttga gcgaagtagg ttagagaagt
     8341 ttgttactca gttgccaaat ggttttgaat tacgtgttat tgatgagaaa gtttacaact
     8401 cttgcttaga aaaggaatgg tcacaggatt tagtagcaaa ttacgctact tatcagtact
     8461 ataaaaaaca aggtataggt tatgttatat attatcaggg gaatatcatt gcaggagctt
     8521 catcttattc aacctataaa aatgggatcg agatagaagt agatacgcat ccagattttc
     8581 gtcggcgtgg tttagcaaca atagttgcag ctcagttgat actaacttgt ttagataagg
     8641 gaatttatcc tagctgggat gctcatacaa ggacttcatt gaatttatct gaaaaactcg
     8701 gttatgaatt ttctcatgaa tacatagcct atgaaataga ttgaagtaag aaaaggagaa
     8761 ctcatgacta aacgtgcttt aatctcagtt tctgacaaat caggaattat tgactttgca
     8821 aaagaattga aaaacttggg ttgggatatt atctcaactg gtgggactaa ggttgccctt
     8881 gacgatgctg gtgttgagac cattgctatt gacgatgtaa ctggctcccc agaaatgatg
     8941 gatggtcgtg ttaagaccct ccactcaaac attcacggtg ggattttggc tcgtcgcgac
     9001 gctgacagcc accttcaggc tgctaaggac aacaacatcg agttgattga cctcgtggtt
     9061 gtcaacctct atcccttcaa ggagactatc cttcgcccag acgtgaccaa cgatttggcg
     9121 gtggaaaaca tcgacatcgg cggtccatca atgcttcgct cagctgctaa aaaccacgct
     9181 agcgtaaccg ttgtggttga tccagctgac tatgccactg ttttgggaga attggctgac
     9241 gctagtcaga cgacatttaa aactcgtcaa cgcttggcag ctaaggcctt ccgtcatacg
     9301 gcagcctacg acgctttgat tgctgagtac ttcacagctc aagtgggaga ggctaagcct
     9361 gaaaaattga ccatcactta tgatcttaaa caggctatgc gttacggaga aaatccacag
     9421 caagacgctg atttctacca aaaagcctta ccaacagact actcaatcgc ttcagctaaa
     9481 cagctcaatg gaaaagaatt gtccttcaat aatatccgtg atgctgatgc agcaatccgt
     9541 attatccgcg atttcaaaga cagtccaacg gttgttgccc tcaaacacat gaacccatgt
     9601 ggtatcggac aggctgatga tattgagaca gcttgggatt acgcttatga agctgatcca
     9661 gtttcaatct ttggtggaat tgttgtcctt aaccgtgaag ttgacgcagc gacagctgag
     9721 aagatgcacc ctatcttctt ggaaatcatc atcgcaccat catactcaga agaagcgcta
     9781 gctattctca caaataaaaa gaaaaacttg cgtatccttg agttgccgtt tgatgcccaa
     9841 gctgccagcg aagtggaagc tgagtacact ggcgtagttg gtggactttt ggtgcaaaac
     9901 caagacgttg tggctgaaaa tccatctgac tggcaagtgg tgacagaccg ccagccaaca
     9961 gaacaagagg tgactgccct tgagtttgcc tggaaggcta tcaagtatgt taagtctaac
    10021 gggattatta ttactaacga tcacatgacg cttggactgg gtgtaggtca aaccaaccgt
    10081 gtcggctcag tcaagattgc tatcgagcag gctaaggacc accttgacgg tgccgttcta
    10141 gcatcagatg ccttcttccc atttgcggac aacattgaag aaatagctgc cgcaggcatc
    10201 aaagcaatca tccagccagg tggttcagtt cgtgatcaag aatctattga cgccgcaaac
    10261 aaacatggct tgaccatgat cttcacaggc gtgagacatt ttagacatta aaaaatagta
    10321 gtttaggttt tcttaagtta actcaggtat actacgtata tcctaaaaac ttttcttttt
    10381 attaaacctc ctaagtactc atcaaggtga gtactttttt tgatattttg atttaaattt
    10441 tcgaataagt taattgcaag tgtttgagca cttcattata ttaattctat ttggaggttt
    10501 ttcttgaata aatggttagt taaggcaagt tccttagttg ttttaggtgg tatggtttta
    10561 tctgcgggtt cccgagtttt agcggatact tatgtccgtt caattgataa tggtagaatt
    10621 acaacaggtt tcaatggtta tcctggacat tgtggggtgg attatgctgt tccgactgga
    10681 acgattatta gggcagtggc agatggtact gtgaaatttg caggagctgg agccaacttt
    10741 tcttggatga cagacttagc aggaaattgt gtcatgattc aacatgcgga tggaatgcat
    10801 agtggttacg ctcatatgtc acgtgtggtg gctaggactg gggaaaaagt caaacaagga
    10861 gatatcatcg gttacgtagg agcaactggt acggcgacgg gacctcacct tcattttgaa
    10921 tttttaccag ctaaccctaa ttttcaaaat ggtttccatg gacgtatcaa tccaacgtca
    10981 ctaattgcta acgttgcgac ctttagtgga aaaacgcaag catcagctcc aagcattaag
    11041 ccattacaat cagctcctgt acagaatcaa tctagtaaat taaaagtgta tcgagtagat
    11101 gaattacaaa aggttaatgg tgtttggtta gtcaaaaata acaccctaac gccgactggg
    11161 tttgattgga acgataatgg tataccagca tcagaaattg atgaggttga tgctaatggt
    11221 aatttgacag ctgaccaggt tcttcaaaaa ggtggttact ttatctttaa tcctaaaact
    11281 cttaagactg tagaaaaacc catccaagga acagctggtt taacttgggc taagacacgc
    11341 tttgctaatg gtagttcagt ttggcttcgc gttgacaaca gtcaagaact gctttacaaa
    11401 tagtttgagg tattgattca ttgttttaaa tgacagtttt gttactaact aagtacaatt
    11461 tctttaaacc gtctgaaaat aattttatag tccagtaaag tgtgatatta tagtctcgga
    11521 ctaataaaaa ggaaatagga attgaagcaa tgaaaatgaa taaaaaggta ctattgacat
    11581 cgacaatggc agcttcgcta ttatcagtcg caagtgttca agcacaagaa acagatacga
    11641 cgtggacagc acgtactgtt tcagaggtaa aggctgattt ggtaaagcaa gacaataaat
    11701 catcatatac tgtgaaatat ggtgatacac taagcgttat ttcagaagca atgtcaattg
    11761 atatgaatgt cttagcaaaa attaataaca ttgcagatat caatcttatt tatcctgaga
    11821 caacactgac agtaacttac gatcagaaga gtcatactgc cacttcaatg aaaatagaaa
    11881 caccagcaac aaatgctgct ggtcaaacaa cagctactgt cgatttgaaa accaatcaag
    11941 tttttgttgc agaccaaaaa gtttctctca atacaatttc ggaaggtatg acaccagaag
    12001 cagcaacaac gattgtttcg ccaatgaaga catattcttc tgcgccagct ttgaaatcaa
    12061 aagaagtatt agcacaagag caagctgtta gtcaagtagc agctaatgaa caggtatcac
    12121 cagctcctgt gaagtcgatt acttcagaag ttccagcagc taaagaggaa gttaaaccaa
    12181 ctcagacgtc agtcagtcag ttaacaacag tatcaccagc ttctgttgcc gctgaaacac
    12241 cagctccagt agctaaagta gcaccggtaa gaactgtagc agcccctaga gtggcaagtg
    12301 ctaaagtagt cactcctaaa gtagaaactg gtgcatcacc agagcatgta tcagctccag
    12361 cagttcctgt gactacgact tcaccagcta cagacagtaa gttacaagcg actgaagtta
    12421 agagcgttcc ggtagcacaa aaagctccaa cagcaacacc ggtagcacaa ccagcttcaa
    12481 caacaaatgc agtagctgca catcctgaaa atgcagggct ccaacctcat gttgcagctt
    12541 ataaagaaaa agtagcgtca acttatggag ttaatgaatt cagtacatac cgtgcgggag
    12601 atccaggtga tcatggtaaa ggtttagcag ttgactttat tgtaggtaaa aaccaagcac
    12661 ttggtaatga agttgcacag tactctacac aaaatatggc agcaaataac atttcatatg
    12721 ttatctggca acaaaagttt tattcaaata caaatagtat ttatggacct gctaatactt
    12781 ggaatgcaat gccagatcgt ggtggcgtta ctgccaacca ctatgaccac gttcacgtat
    12841 catttaacaa ataatataaa aaaggaagct atttggcttc ttttttatat gccttgaata
    12901 gactttcaag gttcttatat aatttttatt aaattgagga gattaagcta taagtctgaa
    12961 actactttca cgttaaccgt gactaaatca aaacgttaaa actaaaatct aagtctgtaa
    13021 agattattga aaacgcttta aaaatagata taataaggtt tgtagatatc taaaattaaa
    13081 aaagataagg aagtgagaat atgccacatc taagtaaaga agcttttaaa aagcaaataa
    13141 aaaatggcat tattgtgtca tgtcaagctt tgcctgggga gcctctttat actgaaagtg
    13201 gaggtgttat gcctctttta gctttggcag ctcaagaagc aggagcggtt ggtataagag
    13261 ccaatagtgt ccgcgacatt aaggaaattc aagaagttac taatttacct atcatcggca
    13321 ttattaaacg tgaatatcct ccacaagaac catttatcac tgctacgatg acagaggtgg
    13381 atcaattagc tactttagat attgcagtaa tagccttaga ttgtacactt agagagcgtc
    13441 atgatggttt gagtgtagct gagtttattc aaaagataaa aaggaaatat cctaaacagt
    13501 tgttaatggc tgatataagt acttttgaag aaggcaaaaa tgcttttgaa gcaggagttg
    13561 attttgtggg tacaactcta tcgggataca cagattacag ccgccaagaa gaaggaccgg
    13621 atatagaact ccttaataag ctttgtcaag ccggtataga tgtgattgcg gaaggtaaaa
    13681 ttcatactcc taagcaggct aatgaaatta atcatatagg tgttgcagga attgtagttg
    13741 ggggtgctat cactagacca aaagaaatag cggagcgttt catctcagga cttagttaaa
    13801 agtgttactc aaaaatcaaa ataaaaataa aaaaggggaa tagttatgag tatcaaaaaa
    13861 agtgtgattg gtttttgcct cggagctgca gcattatcaa tgtttgcttg tgtagacagt
    13921 agtcaatctg ttatggctgc cgagaaggat aaagtcgaaa ttacgtggtg ggcttttcca
    13981 acctttactc aagaaaaggc taaggatgga gtaggtactt atgagaaaaa agtcatcaag
    14041 gcttttgaaa agaaaaatcc taatataaaa gtaaaactag agacaattga tttcacatct
    14101 ggacctgaaa aaatcactac agcaattgaa gcagggacag cacctgatgt gctttttgat
    14161 gcaccagggc gaattattca atatggtaaa aatggtaaat tagcagattt gaatgattta
    14221 tttacagacc aatttattaa ggatgtcaat aataagaaca tcattcaagc ttctaagtct
    14281 ggcgataaag cctacatgta tccaataagt tctgccccat tttatatggc gttcaataaa
    14341 aaaatgctta aagatgcagg agttttgaaa cttgtaaaag aaggttggac tactagtgat
    14401 tttgaaaaag tactaaaagc actaaaaaat aaaggctata caccaggttc attctttgca
    14461 aacgggcaag gaggagatca aggaccacgt gcattttttg ctaatcttta tagtgctcca
    14521 ataacagata aagaagtaac aaaatatacc actgacacta aaaattctgt aaaatcaatg
    14581 aaaaaaatag ttgaatggat taagaaaggc tacttgatga atgggtctca gtatgatggc
    14641 tcagctgaca ttcaaaactt cgccaatgga caaactgctt tcactatcct atgggctcca
    14701 gctcaaccaa aaactcaagc aaaattatta gagtcaagta aagtggatta ccttgaagtg
    14761 ccattcccat cagaagatgg aaaaccagat ttagaatacc ttgttaatgg ttttgcggtc
    14821 tttaataata aagatgaaaa caaagtaaaa gcctctaaga aatttatcac ttttattgct
    14881 gatgataaaa aatggggacc aaaagatgtt atacgtacag gtgctttccc agttagaaca
    14941 tcatttgggg atctttataa aggtgataaa cgtatgatga agatttcaaa atggactcaa
    15001 tattattcac catattacaa cactatcgat ggattttctg aaatgagaac cttatggttc
    15061 ccaatggttc aatctgtatc caatggtgat gaaaaaccag cagatgcttt gaaagacttt
    15121 actcaaaaag caaatgatac cattaaaaaa gcagctaaat aattaaggag actccttttc
    15181 tatggtcaat cacatattgg gatagaagag gagtttttag tcaagttaag atgtaaagga
    15241 gagtaccgta tgcgtactaa taagttaaag atgagggaaa cgatgatagc ttatgctttt
    15301 ctagcgccta ttctcttatt tttcttaatt tttgtttttg caccgatggt tatgggcttt
    15361 gtcactagtt tttttaatta ttctatgaca cagttcactt ttattgggtt agctaattac
    15421 aatcgtatgt ttcatgattc tattttcatg aaatccttga taaatacagt aatcattgtg
    15481 attggctctg tgccagtagt ggttttcttc tcactttttg ttgctgccaa tacttatgaa
    15541 aaaaatgttt tttcacgctc tttctaccgt tgtgtcttct tcttgccagt agtaactggt
    15601 agtgttgcag tcacagttgt ttggaaatgg atttatgacc ctatgtccgg aattttaaat
    15661 tacatcttga aatcaggtca tgtcattgaa cagaatatta gctggcttgg tgataagcac
    15721 tgggcattac ttgctattat tattattttg ctaacgacct cagttggtca acctattatt
    15781 ctatatattg cggcgatggg aaatattgat aattctcttt gcgaagctgc gcgtgttgat
    15841 ggagccaatg aaatgcaagt tttttggcaa attaaatggc cgagtctctt accaactaca
    15901 ctctatattg cagttataac aacaattaat tcatttcaat gttttgcctt aatccagctc
    15961 ctaacatctg gtggaccaaa ttattcaaca agcactttga tgtattatct ttatgaaaaa
    16021 gcctttaaac tatcagaata tggctatgct aatactatgg gagtattcct agcagtaatg
    16081 attgctttaa taagttttgc tcaatttaag atcttaggaa atgatgtgga atattaggag
    16141 gaaaacatga aaaagaaaac attcagtgct tataactttt taacggctct tatcctttgt
    16201 cttttgacag tgctttttat ctttccattt tattggatta tgacaggagc ttttaaatct
    16261 caacctgata ctattatcat ccccccacaa tggtggccca aagcaccgac cttagaaaat
    16321 tttaaagctt taactgtaca aaacccagct ttgagatggc tttggaatag tgtctttatc
    16381 tcaattatga caatgttcct agtttgctgt acatcgtcaa tggcaggcta tgttttagct
    16441 aagaaacgat tttacggtca aaaaatattg ttctcccttt ttattgctgc tatggcttta
    16501 cctaaacaag ttgttttagt gccattagtt agaattatca attttatggg gatacatgat
    16561 accttatggg cggttatttt acctcttgtt ggttggcctt ttggggtctt cttgatgaaa
    16621 cagttttctg aaaatatttc aacagaatta ctggaatcag ctaaaattga tggttgtgga
    16681 gagattcgaa catttattaa tgtagcattt ccaattgtca aacctggatt tgctgcccta
    16741 gctattttca catttatcaa tacttggaat gactatttta tgcaattagt tatgctaact
    16801 tctagaaata atctgaccat ttctcttggg gtagctacta tgcaggctga aatggcaaca
    16861 aactatggct taattatggc aggtgcagcc ttagctgctg ttcctattgt gacagtattc
    16921 ttagttttcc aaaaatcctt tactcagggg attacaatgg gagctgttaa aggataggta
    16981 ggatttatta tgatttacga tcatttattg aatttaacgc attataaaga tattaatcct
    17041 aatttagatt tagcgattga ttatctacta agtcatgatt taaggaattt agatataggc
    17101 acctatcata ttagcccaga agtgatactc atggtccaat caaatcaact tagtgagtca
    17161 tttgatcata tttttgaata ccataaaaaa taccttgata tacattatgt cattgaagga
    17221 tatgaagtta tcaaattggg aaaaggtgat aaggtagagg tagaggaata cttaggcgat
    17281 ataggatttg tcaagtgctc agaagagact tcttttgatt tgagagataa ttatatagcc
    17341 ttctttttcc cagaagaagc tcatcagcca aatggtatgg gaagtctagg taattatgtc
    17401 aaaaaagggg tattgaaagt gttaatggcg taagaaatac agggagtata ttgtgaaaaa
    17461 agcaaatcaa ttaatagcag cgatttttga tcttaacaac ccctttatgc aaggttgcaa
    17521 tgttgttttt gatctggcat tgctaaacct tctctttatg ataacttgcc taccattggt
    17581 aactattggt gctgctaaaa ttagtttgta tcgaacgttg tggcaaaaat tagaaggtga
    17641 tcagacaaat cttttaatat tgtatataaa acacctaaaa aaagagtggt tccaaggtat
    17701 gcttttaggt ttggttgaac taagtatttt ggttgtgatc atatttgatt taacaattct
    17761 tcattatcaa ataggtttca ttgtgccttt cttgaagata atttgttacg cctttttgtt
    17821 attgactgtg atgacgagca tatacctatt tcctatggct gcccgctatg aaatgtctct
    17881 tttagatacc gtgaaaaaga gctttataat ggcatgttta aacttgaaat ggactggtgt
    17941 attgatgttt ttattaatca tgacttggtt tattatggtg cagtctagtc tcttatt
//
LOCUS       DATROQ010000038        17734 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.038, whole genome shotgun sequence.
ACCESSION   DATROQ010000038 DATROQ010000000
VERSION     DATROQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17734
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.038"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            74..283
                     /locus_tag="VBC94_001125"
     CDS             74..283
                     /locus_tag="VBC94_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEO6979400.1"
                     /translation="MKVLFDVQNLLKKFGIYVYIGKRLYDIEVMKIELQRLYDNGLIS
                     RDDYLKAELILRREHRLELEKENKK"
     gene            280..1248
                     /locus_tag="VBC94_001126"
     CDS             280..1248
                     /locus_tag="VBC94_001126"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEO6979401.1"
                     /translation="MSKKLLGIDLGGTTIKFGILTLEGEVQEKWAIETNTLENGRHIV
                     SDIVESLKHRLSLYGLTKDDFLGIGMGSPGAVDRTSKTVTGAFNLNWADTQEVGSVIE
                     KEVGIPFFIDNDANVAALGERWVGAGANNPDVVFVTLGTGVGGGVIADGNLIHGVAGA
                     GGEIGHMIVDPENGFTCTCGNKGCLETVASATGVVRVARQLAEQYEGSSAIKAAIDNG
                     DTVTSKDIFIAAEDGDKFANSVVERVSRYLGLAAANISNILNPDSVVIGGGVSAAGEF
                     LRSRVEKYFVTFAFPQVKKSTKIKIAELGNDAGIIGAASLANQQAS"
     gene            1260..1640
                     /locus_tag="VBC94_001127"
     CDS             1260..1640
                     /locus_tag="VBC94_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO6979402.1"
                     /translation="MDMSVILIIVILLAFVAWASWNYWRVRRAAKFLDNESFQKEMSR
                     GQLIDIREAGAFHRKHILGARNIPASQFKVALSALRKDKPVLLYDASRGQSIPRIVLL
                     LRKEGFNQLYVLKDGFNYWTGRVK"
     gene            1872..3713
                     /gene="typA"
                     /locus_tag="VBC94_001128"
     CDS             1872..3713
                     /gene="typA"
                     /locus_tag="VBC94_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEO6979403.1"
                     /translation="MTNLRTDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELEER
                     AMDSNDIEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPSEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSMSDDPSDQEKTMAPIFDTIIDHIPAPVDNSEEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERKEIQEAKAG
                     DLIAVSGMEDIFVGETVTPTDAIEPLPVLRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVQCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGVLVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLADDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            3758..4003
                     /locus_tag="VBC94_001129"
     CDS             3758..4003
                     /locus_tag="VBC94_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEO6979404.1"
                     /translation="MFYLIVALLIALYYFFMAPKTVRNTLNAIGLVGLVALLLVLAVM
                     SFIKILQLPGELYIGLIMIPLGYTAFKETLNLSEKKK"
     gene            4133..5488
                     /locus_tag="VBC94_001130"
     CDS             4133..5488
                     /locus_tag="VBC94_001130"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEO6979405.1"
                     /translation="MKTITTFENKKVLVLGLARSGEAAARLLAKLGAIVTVNDGKPFD
                     ENPTAQSLLEEGIKVVCGSHPLELLDEDFCYMIKNPGIPYNNPMVKKALEKQIPVLTE
                     VELAYLVSESQLIGITGSNGKTTTTTMIAEVLNAGGQRGLLAGNIGFPASEVVQAADD
                     KDILVMELSSFQLMGVKEFRPHIAVITNLMPTHLDYHGSFEDYVAAKWNIQNQMSSSD
                     FLVLNFNQGISKELAKTTKATIVPFSTTEKVDGAYVQDKQLFYKGENIMSVDDIGVPG
                     SHNVENALATIAVAKLAGISNQVIRETLSNFGGVKHRLQSLGKVHGISFYNDSKSTNI
                     LATQKALSGFDNTKVILIAGGLDRGNEFDELIPDITGLKHMVVLGESASRVKRAAQKA
                     GVTYSDALDVRDAVHKAYEVAQQGDVILLSPANASWDMYKNFEVRGDEFIDTFESLRG
                     E"
     gene            5491..6567
                     /locus_tag="VBC94_001131"
     CDS             5491..6567
                     /locus_tag="VBC94_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEO6979406.1"
                     /translation="MGKKIVFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     EQINQSGLDITFHSIATGKLRRYFSWQNMLDVFKVGVGVLQSIAIIAKLRPQALFSKG
                     GFVSVPPVVAARLLKVPVFVHESDLSMGLANKIAYKFATIMYTTFEQSKDLIKTKHIG
                     AVTKVMDCKKSFENTDLTSIKEAFDPNLKTLLFIGGSAGAKVFNDFITQTPELEEKYN
                     VINISGDSSLNRLKKNLYRVDYVTDLYQPLMNLADVVVTRGGSNTIFELVAMKKLHLI
                     IPLGREASRGDQLENAAYFEEKGYALQLPESELNINTLEKQINLLISNSESYEKNMSQ
                     SSEIKSQDEFYQLLIDDMAKVTKG"
     gene            6571..7707
                     /locus_tag="VBC94_001132"
     CDS             6571..7707
                     /locus_tag="VBC94_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEO6979407.1"
                     /translation="MPKKKSDTPEKEEVVLTEWQKRNLEFLKKRKEDEEEQKRINEKL
                     RLDKRSKLNISSPEEPQNTTKIKKLHFPKISKPKIEKKQKKEKIVNSLAKTNRIRTAP
                     IFVVAFLVILVSVFLLTPFSKQKTITVSGNQHTPDDILIEKTNIQKNDYFFSLIFKHK
                     AIEQRLAAEDVWVKTAQMTYQFPNKFHIQVQENKIIAYAHTKQGYQPVLETGKKADPV
                     NSSELPKHFLTINLDKEDSIKLLIKDLKALDPDLISEIQVISLADSKTTPDLLLLDMH
                     DGNSIRIPLSKFKERLPFYKQIKKNLKEPSIVDMEVGVYTTTSTIESTPVKAEDTKNK
                     STDKTQTQNGQVAENSQGQTNNSNTNQQGQQIATEQAPNPQNVN"
     gene            7980..9353
                     /gene="ftsA"
                     /locus_tag="VBC94_001133"
     CDS             7980..9353
                     /gene="ftsA"
                     /locus_tag="VBC94_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEO6979408.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFIANEMNVIGVSNVPSSGVKD
                     GIIIDIEAAATAIKEAVKQAEEKAGITIDKINVGLPANLLQIEPTQGMIPVPNESKEI
                     KDEDVESVVKSALTKSITPEREVISLIPLEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPTTILHNLRKTVERAGIKVEHVVIAPLALAKSVLNEGEREFGATVIDMGGGQTTVAS
                     MRNQELQYTNIYSEGSDYVTKDISKVLRTTVEIAEALKFNFGQANVEEASTSDTVQVN
                     VVGNEEPVEITESYLSQIISGRIRQILEHVKQDLGRGRLLDLPGGIILVGGGAIMPGV
                     VEVAQQIFGTRVKLHVPNQVGIRNPMFANVISIVDYVGMMSEVDIIAQHAVTGDEMLR
                     HKPVDFDYKEKTNTMSTMPYSEPLTSSMEDSNLEPIRARENAQEPTEPKTNIGERIRG
                     IFGSMFD"
     gene            9375..10655
                     /gene="ftsZ"
                     /locus_tag="VBC94_001134"
     CDS             9375..10655
                     /gene="ftsZ"
                     /locus_tag="VBC94_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEO6979409.1"
                     /translation="MVFSFDTASVQGAVIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEVLTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVITRPFGFEGNKRSNFAIEGIQELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERITEAARKAIYSPLLETTIDGAEDVIVNVTGGMDMTLTEA
                     EEASEIVSQAAGKGVNIWLGTSIDMDMKDEIRVTVVATGVRKDKTNQVSGFTTSAPTN
                     QAPSERQSTSNSNFDRRGNFDMTESREMPTQQNQPHAQNQQQSSAFGNWDLRRDNISR
                     PTEGELDSKLSMSTFSENDDMDDELETPPFFKNR"
     gene            10661..11335
                     /locus_tag="VBC94_001135"
     CDS             10661..11335
                     /locus_tag="VBC94_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEO6979410.1"
                     /translation="MMNLQENKTAIFDNVSKLALKAGRAPESVHIVAVTKYVNCQTTE
                     ALIRTGVNHIGENRVDKFLEKYQALKDEKLTWHLIGSLQRRKVKDVINYVDYFHALDS
                     VKLAAEIQKRAQKPVKCFLQVNISREDSKHGFTIEQIDDALNLISRYDKIELIGIMTM
                     APLKATKEEISSIFEETESLRKRLQARNIERMPFTELSMGMSRDYDIAIQNGSTFVRI
                     GTSFFK"
     gene            11347..11952
                     /locus_tag="VBC94_001136"
     CDS             11347..11952
                     /locus_tag="VBC94_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEO6979411.1"
                     /translation="MALKDRFDKIISYFDTDDVSENEVHEVQERTSVQRDSRAATAQE
                     ASQRSHMTNSAEEEMIGSRPRTSTYNPNRQERQRVQRDNAYQQATPRIQNKDSVRQQR
                     EQVTIALKYPRKYEDAQEIVDLLIVNECVLIDFQYMLDAQARRCLDYIDGASRVLYGS
                     LQKVGSSMFLLTPANVMVDIEEMNIPKTGQETSFDFDMKRR"
     gene            11955..12209
                     /locus_tag="VBC94_001137"
     CDS             11955..12209
                     /locus_tag="VBC94_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEO6979412.1"
                     /translation="MILVLLILLKTIRVYSYILLAYALLSWLPGAYDSWIGRLVVQIV
                     DPIIRPLRRFNLQFLGLDFTVIVAFFLLNMLSRFLILLFT"
     gene            12211..12999
                     /locus_tag="VBC94_001138"
     CDS             12211..12999
                     /locus_tag="VBC94_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEO6979413.1"
                     /translation="MTLDDIYQHFRPEEYAFINKIDHLAQYVENTYSFITTEFLNPRE
                     FKILESVLERRGSHYYTSGQYFQTEYVKVIIAPEYYQLDMVDFNLSLIEIKYNAKFNH
                     LTHAKIMGTLLNYLGVKRSILGDILVEEGCAQVLVDSQMTNHLVHSVTKIGTASVQLA
                     EVPLSKLLTPKQDIQKLTVIASSLRLDKILATILKISRTQSTKLIEADKVKVNYAIVN
                     RVSEQLVEGDLISVRGYGRFTLNHNLGLTKNQKYKLEVDKMIHN"
     gene            13009..13779
                     /locus_tag="VBC94_001139"
     CDS             13009..13779
                     /locus_tag="VBC94_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEO6979414.1"
                     /translation="MPLTALEIKDKTFSSKFRGYSEEEVNEFLEIVVDDYEDLIRRNR
                     EQEQYIKDLEEKIAYFNEMKESLSQSVILAQETAERVKISAQDEASNLMGKATFDAQH
                     LIDEAKLKANQILRDATDDAKRVAIETEDLKRQSRVFHQRLLSELEGQLKLANSSAWE
                     ELLKPTAIYLQNSDASFKEVVEKVLDEDDALPVVDDTESFDATRQFSPDEMEELQRRV
                     EESNKQLEESGLLDTNNFQMEEPINLGETQTFKLNIED"
     gene            14064..16856
                     /locus_tag="VBC94_001140"
     CDS             14064..16856
                     /locus_tag="VBC94_001140"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEO6979415.1"
                     /translation="MKLKETLNLGQTAFPMRAGLPNKEPQWQEAWDQADIYKKRQALN
                     EGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFRAPYVPGWDTHGLPIE
                     QVLAKKGVKRKEMDLAEYLEMCRDYALSQVDKQRDDFKRLGVSADWENPYITLTPDYE
                     ADQVRVFGAMADKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTVTASRGLTVGPDMEYVVVAPVGSERKYLLAEVLVDSLAAK
                     FGWENFEIVTHHTGKELNHIVTEHPWDTEVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLDVVVTVDSRGLMMENAGPDFEGQFYDKVTPLVKEKLGDLLLASEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKTNFQPEWGKKRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFAEYGSIVWWQRDAKDLLPAGY
                     THPGSPNGLFEKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAVLSQGFVLDGKGEKMSKSLGNTILPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILKQTSETYRKIRNTLRFLIANTSDFNPKQDAVAYENLGAVDRYMT
                     IKFNQVVDTINKAYAAYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSPERRR
                     MQTVFYDILVKLTKLLTPILPHTAEEIWSYLEHEEEEFVQLAEMPVAQTFSGQEEILE
                     EWSAFMTLRTQAQKALEEARNAKVIGKSLEAHLTIYASQEVKTLLTALNSDIALLMIV
                     SQLTIADEADKPADSVSFEGVAFTVEHAEGEVCERSRRIDPTTRMRSYGVAVCDASAA
                     IIEQYYPEAVAQGFEA"
     gene            complement(16926..17228)
                     /locus_tag="VBC94_001141"
     CDS             complement(16926..17228)
                     /locus_tag="VBC94_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEO6979416.1"
                     /translation="MRLINTTSSHPELVRNQLQNTDAKLVEVYSAGNTDVVFTTAPKH
                     YELLISNKYRAIKDEELEAIREFFLKRKIDQSIIIQEQMKSLHTAKLIEISYPTTA"
     gene            complement(17292..>17734)
                     /locus_tag="VBC94_001142"
     CDS             complement(17292..>17734)
                     /locus_tag="VBC94_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6979417.1"
                     /translation="FGEKIDNVDYRSRFGVYAIIPNPTHDKIILVQAPNGAWFLPGGE
                     IEENENHLEALTRELIEELGYSATIGHYYGQADEYFYSRHRDTYYYNPAYIYEVTAYH
                     KEQAPLEDFNHLAWFPIQEAKEKLKRGSHRWGVQAWEKNHHSRK"
BASE COUNT         5905 a         2816 c         3586 g         5427 t
ORIGIN      
        1 acgtcataat aacaattagc aagcggttgc ggattttctt gaaaattgaa tggttttctg
       61 ttaaaattca tatatgaaag ttttattcga tgtgcagaat cttttgaaaa aatttggtat
      121 ttatgtttat attggtaaac gtctttatga tatcgaagtt atgaagattg aattgcaacg
      181 tctctacgat aatggtctga taagtagaga tgactattta aaagcagaac tgattttaag
      241 aagagagcac agactagaat tggagaagga aaataaaaaa tgagtaagaa attattggga
      301 attgacctcg gaggaacgac cattaaattt ggtatcttga cgcttgaggg agaagtacaa
      361 gaaaaatggg caattgagac caatacttta gaaaacggaa gacatatcgt ttctgatatc
      421 gttgaatctc tcaaacatcg tttgagcctc tatggattaa caaaagatga ctttctcggt
      481 atcggtatgg gttctccagg agctgttgat agaactagta aaacagtaac aggtgctttt
      541 aatctaaatt gggctgatac tcaagaagta ggttcggtta ttgaaaaaga agttggaatt
      601 ccatttttta ttgataacga tgctaatgtt gcagcacttg gtgaacgctg ggtaggtgct
      661 ggtgccaata atcccgatgt tgttttcgta accctcggaa caggagtagg tggaggtgtt
      721 atcgcagatg gtaacctcat ccatggtgtt gcaggagcag gtggagaaat tgggcatatg
      781 attgttgatc cagaaaatgg atttacgtgc acatgtggta acaaaggctg tcttgagaca
      841 gttgcatcag cgacaggtgt tgttagagta gcacgtcaac tcgcagaaca atatgaaggt
      901 tcgtctgcca ttaaagcagc gattgacaac ggtgatactg ttacaagtaa agatattttt
      961 atagcagcag aagatgggga taaatttgct aattctgttg ttgaacgtgt atcacgttac
     1021 cttggactgg cagcagctaa tatttcaaat attttaaacc ctgattctgt ggttattggt
     1081 ggcggtgtct cagcagcagg tgaattttta cgtagtcgcg ttgagaaata ctttgtcaca
     1141 tttgctttcc cacaagttaa aaagtcaact aaaattaaga ttgctgaact aggtaatgat
     1201 gctggtatta ttggtgcagc aagcttagcc aatcaacaag caagttaagg agataagata
     1261 tggatatgtc tgttatttta attatcgtta ttcttctggc ttttgttgct tgggcatctt
     1321 ggaattattg gagggttcgt agggcagcta aatttttaga taacgagtct tttcaaaaag
     1381 aaatgtcacg aggacagtta attgatatcc gagaagctgg tgcttttcat agaaagcata
     1441 ttctcggagc acgtaacatt ccagcaagtc aatttaaagt tgccttatcg gctttacgca
     1501 aagataaacc tgtcctctta tatgacgcaa gccgtggtca gtctattcca aggattgttt
     1561 tgttactcag aaaagaaggt tttaatcaac tttacgtgtt aaaggacggt ttcaattact
     1621 ggacgggtag agttaagtaa tatactaagg agagagttac tattaataac tctcttctct
     1681 ttttgtagaa atgaattaaa ttgaaaaaag tagaaaataa atgacataaa taatgaaatg
     1741 atgaaaagtt ttcctatcac aaataggcag ttaatatgaa aacatttaca cttgtgtaaa
     1801 ttctgttttt taagaaaaat tgtgttataa ttcataagtt aacagaatta cattataaaa
     1861 tagaggaaaa catgacaaat ttaagaacag atatccgtaa cgttgcgatc attgcccacg
     1921 ttgaccacgg taaaacaact ctcgttgatg aattattaaa acaatcacat actcttgatg
     1981 agcgtaaaga gcttgaagaa cgtgcaatgg attcaaatga tatcgagaaa gaacgtggta
     2041 tcaccattct tgcaaaaaat acagccgtag catacaacga tgttcgtatc aatattatgg
     2101 acacacctgg tcacgcggac tttggtggtg aagttgagcg tattatgaaa atggttgatg
     2161 gtgttgtttt agtcgttgat gcttacgaag gaacaatgcc acaaacacgt tttgttttga
     2221 agaaagctct tgaacaaaac ttaattccaa tcgttgttgt aaataaaatt gataagccat
     2281 cagctcgtcc atcagaggtt gttgatgaag ttcttgaact cttcattgag ctcggtgctg
     2341 atgatgacca actagatttc cctgttgttt atgcttcagc catcaatgga acatcttcaa
     2401 tgtcagatga tccttcagat caagaaaaaa caatggcacc gatttttgat actatcattg
     2461 atcacattcc agccccagtt gacaactcgg aagaaccact tcaattccaa gtttctcttc
     2521 ttgattacaa tgattttgta ggacgtattg gtattggacg tgttttccgc gggactgtca
     2581 aagttggaga tcaagttact ctttcaaaac ttgatggtac aactaaaaac ttccgcgtaa
     2641 caaaactttt tggtttcttt ggacttgaac gtaaagaaat ccaagaggct aaagcgggtg
     2701 atttaatcgc tgtttctggt atggaagata tcttcgttgg tgagacagta actccgacag
     2761 atgctattga accactacca gttttacgta ttgacgagcc aacacttcaa atgactttct
     2821 tggtgaataa ttcaccattt gcaggtcgcg aaggtaaatg gattacgtca cgtaaggttg
     2881 aagaacgtct tttagcagaa ttacaaacag acgtttcttt gcgtgttgac ccaacagatt
     2941 cgccagataa atggacggtt tcagggcgtg gagaattaca tttatctatc ctcattgaaa
     3001 caatgcgtcg tgagggatat gaacttcaag tatcacgtcc agaagttatc atcaaagaaa
     3061 ttgatggtgt tcaatgcgag ccgtttgagc gtgttcaaat tgatactcca gaagaatatc
     3121 agggtgctat tatccaaagt ttgtcagagc gtaaaggtga tatgcttgat atgcagatgg
     3181 ttggtaatgg tcaaacgcgt ttgattttct tgattcctgc acgtggtttg attggttatt
     3241 caacagagtt tctttcaatg acacgtggat atggtatcat gaatcatact tttgaccagt
     3301 atctaccggt tgttcaagga gaaattggtg gtcgtcatcg tggtgtcttg gtttctattg
     3361 aaaatggtaa agcaactaca tattcaatta tgcgtattga agaacgtggg actatctttg
     3421 taaatccagg tacagaagtt tatgaaggaa tgattgttgg tgagaattct cgtgataatg
     3481 atctcggagt caatattaca actgctaaac aaatgacaaa tgtccgttca gcaactaaag
     3541 atcaaactgc agtcatcaag acgcctcgta tccttacttt agaagagtca cttgaatttt
     3601 tagcagatga tgagtacatg gaagtaactc ctgagtctat tcgtcttcgt aaacaaattt
     3661 taaataaagc agcacgtgac aaagctaata aaaagaaaaa atcagctgaa taatatccaa
     3721 agtatttctt atttatgatt aataagaaag gagttctatg ttttacttaa ttgttgccct
     3781 tttgattgcc ttatattact tttttatggc accaaaaaca gtacgtaata ctttgaacgc
     3841 tattggttta gtaggtttgg tagccttatt acttgtcttg gcagttatga gttttattaa
     3901 aatccttcaa ttgccaggtg aattgtatat tgggcttata atgattcctc taggatatac
     3961 agcatttaaa gaaacattga atttgtcaga aaagaagaaa tagcttatga acttaggatt
     4021 ttgtctccta agttcttttg ttttgagaaa ttagagacat atcctttacc ttgaaaaagc
     4081 cactgattaa aggtaaaata atagggataa taatgataga atgggacgag taatgaaaac
     4141 aataacaaca tttgaaaata aaaaagtttt agtccttggt ttagcacgat ctggagaagc
     4201 cgctgcacgt ttgttagcta agttaggagc aatagtgaca gttaatgatg gcaaaccatt
     4261 tgatgaaaat ccaacagcac agtctttgtt ggaagagggt attaaagtgg tttgtggtag
     4321 tcatccttta gaattgttag atgaggattt ttgttacatg attaaaaatc caggaatacc
     4381 ttataacaat cctatggtca aaaaagcatt agaaaaacaa atccctgttt tgactgaagt
     4441 ggaattagca tacttagttt cagaatctca gctaataggt attacaggct ctaacgggaa
     4501 aacgacaacg acaacgatga ttgcagaagt cttaaatgct ggaggtcaga gaggtttgtt
     4561 agctgggaat atcggctttc ctgctagtga agttgttcag gctgcggatg ataaagatat
     4621 tctagttatg gaattatcaa gttttcagct aatgggagtt aaggaatttc gtcctcatat
     4681 tgcagtaatt actaatttaa tgccaactca tttagattat catgggtctt ttgaagatta
     4741 tgttgctgca aaatggaata tccaaaatca aatgtcttca tctgattttt tggtacttaa
     4801 ttttaatcaa ggtatttcta aagagttagc taaaactact aaagcaacaa tcgttccttt
     4861 ctctactacg gaaaaagttg atggtgctta cgtacaagac aagcaacttt tctataaagg
     4921 ggagaatatt atgtcagtag atgacattgg tgtcccagga agccataacg tagagaatgc
     4981 tctagcaact attgcggttg ctaaactagc tggtatcagt aatcaagtta ttagagaaac
     5041 tttaagcaat tttggaggtg ttaaacaccg cttgcaatca ctcggtaagg ttcatggtat
     5101 tagtttctat aacgacagca agtcaactaa tatattggca actcaaaaag cattatctgg
     5161 ctttgataat actaaagtta tcctaattgc aggaggtctt gatcgcggta atgagtttga
     5221 tgaattgata ccagatatca ctggacttaa acatatggtt gttttagggg aatcggcatc
     5281 tcgagtaaaa cgtgctgcac aaaaagcagg agtaacttat agcgatgctt tagatgttag
     5341 agatgcggta cataaagctt atgaggtggc acaacagggc gatgttatct tgctaagtcc
     5401 tgcaaatgca tcatgggaca tgtataagaa tttcgaagtc cgtggtgatg aattcattga
     5461 tactttcgaa agtcttagag gagagtaact atggggaaaa aaattgtttt tacaggtggt
     5521 ggtacggtag gtcatgtaac actaaaccta atattaatcc ccaaatttat caaagatggt
     5581 tgggaagtac attatattgg tgataaaaat ggcatagaac atgaacaaat taatcagtct
     5641 ggacttgata tcacatttca ctctatagct actgggaaac tacgtcgcta tttttcatgg
     5701 caaaatatgt tagatgtctt taaagtaggg gttggcgttc ttcaatcgat tgctattatt
     5761 gctaaactta gaccccaagc cttattttca aaagggggat ttgtttcagt acctcctgta
     5821 gtagctgcac gcctattaaa agttcctgtt tttgttcatg aatcagattt atcaatgggg
     5881 ttagcaaata agattgctta taaatttgcg actattatgt atacgacttt tgaacaatct
     5941 aaagatttaa tcaaaactaa gcatattggt gctgtaacaa aagtcatgga ttgtaaaaag
     6001 tcgtttgaaa atactgactt aactagtata aaagaagcct tcgatccaaa cctaaagacc
     6061 ttactattta ttggaggatc agcaggtgca aaagtattca atgattttat tactcaaaca
     6121 cctgagctgg aagaaaaata taatgttatc aatatttcag gcgattcttc tctcaatcga
     6181 ttgaagaaga acctttatcg cgtggattat gttacagacc tttatcagcc tttaatgaat
     6241 ttagcagatg tggttgttac acgaggtggc tctaatacta tttttgaatt agtagctatg
     6301 aaaaaacttc atcttattat ccctcttgga cgtgaagcga gccgaggtga ccaattagag
     6361 aatgctgctt attttgaaga aaagggctac gcattgcagt taccagaatc tgaattaaac
     6421 ataaatacac ttgagaaaca gataaattta ttaatttcta acagtgagag ttacgaaaaa
     6481 aacatgtcac aatcatctga aataaaatct caagatgaat tttatcaatt attaattgat
     6541 gatatggcaa aagtaacaaa aggataaaac atgcctaaga agaaatcaga taccccagaa
     6601 aaagaagaag ttgtcttaac ggaatggcaa aagcgtaacc ttgaattttt aaaaaaacgc
     6661 aaagaagatg aagaagaaca aaaacgtatt aacgaaaaat tacgcttaga taaaagaagt
     6721 aaattaaata tttcttctcc tgaagaacct caaaatacta ctaaaattaa gaagcttcat
     6781 tttccaaaga tttcaaaacc taagattgaa aagaaacaga aaaaagaaaa aatagtcaac
     6841 agcttagcca aaactaatcg cattagaact gcacctatat ttgtagtagc attcctagtc
     6901 attttagttt ccgttttcct actaactcct tttagtaagc aaaaaacaat aacagttagt
     6961 ggcaatcagc atacacctga tgatattttg atagaaaaaa cgaatattca aaaaaacgat
     7021 tatttctttt ctttaatttt taaacataaa gctattgaac aacgtttagc tgcagaagat
     7081 gtatgggtaa aaacagctca gatgacttat caatttccca ataagtttca tattcaagtt
     7141 caagaaaata agattattgc atatgcacat acaaagcaag gatatcagcc tgtcttggaa
     7201 actggaaaaa aggctgatcc tgtaaatagt tcagagctac caaagcactt cttaacaatt
     7261 aaccttgata aggaagatag tattaagcta ttaattaaag atttaaaggc tttagaccct
     7321 gatttaataa gtgagattca ggtgataagt ttagctgatt ctaaaacgac acctgacctc
     7381 ctgctgttag atatgcatga tggaaatagt attagaatac cattatctaa atttaaagaa
     7441 agacttcctt tttacaaaca aattaagaag aaccttaagg aaccttctat tgttgatatg
     7501 gaagtgggag tttacacaac aacaagtact attgaatcaa cccctgtgaa agcggaagat
     7561 acaaaaaata aatcaactga taaaacacaa acacaaaatg gtcaggttgc ggaaaatagt
     7621 caaggacaaa caaataactc aaatactaat caacaaggac aacagatagc aacagagcag
     7681 gcacccaacc ctcaaaatgt taattagcga taaaaaaaga ctggatttat cagtcttttt
     7741 tatacatatc aagtcagtca tagattgcta gtaaaaattg atttcttgaa aaaaatttaa
     7801 gtttgttata agttgcgaat ggtttaaata agttagaaat atttcaattt gatgttgctt
     7861 aataaaaaaa cgtaatttgg taacaattct gcttttttta tggtataata ttttctgaat
     7921 aattctgttt ttggcagatg tagaatagaa aagaatggaa agatagcgag gtcgagtgaa
     7981 tggctagaaa tggctttttt actggtttgg atataggaac aagctcgatt aaagttttag
     8041 ttgcagaatt tattgcaaat gaaatgaatg taattggggt tagcaacgtc cctagttccg
     8101 gtgtaaaaga tggtataatt attgatattg aggcagcagc aactgcaatc aaagaggcgg
     8161 taaaacaagc tgaggagaaa gcaggcatca ccattgacaa aatcaatgtt ggattaccag
     8221 caaatcttct ccaaattgaa ccaactcaag gaatgattcc tgttcccaat gaatcaaaag
     8281 aaattaagga tgaagatgtc gaaagtgttg ttaaatcagc attgactaaa agcattactc
     8341 ctgaaagaga agttatttca ttgattccgc ttgaattcat tgtagatggt ttccaaggga
     8401 ttagagaccc tagagggatg atggggattc gccttgagat gcgtggtctt atctatactg
     8461 gaccaacaac tatccttcat aatcttcgta aaactgttga acgagcaggt ataaaagtag
     8521 agcatgttgt gattgcccct ctagccttag caaaatcagt tctaaacgaa ggtgaacgtg
     8581 agtttggtgc aactgtgatt gatatgggag gcggacaaac aacagtcgct tctatgcgta
     8641 atcaagaatt gcaatatact aatatttatt ctgaaggtag cgattatgtc accaaagata
     8701 tttctaaagt gttacgtaca acagtagaaa ttgccgaagc attgaaattc aactttggac
     8761 aagctaatgt tgaagaagca agtacttctg atacagttca agttaatgtt gttggtaacg
     8821 aggaacctgt tgaaattaca gaaagctatc tttcgcaaat tatttcagga cgtattcgtc
     8881 aaattttaga acatgtcaaa caagacttgg gtagaggtcg tttacttgat ttacctggtg
     8941 gtattattct tgttggaggt ggtgcaatta tgccaggagt tgtcgaggtt gctcaacaaa
     9001 tttttggaac tagagttaaa ttgcacgttc caaaccaagt aggtattcgc aatccaatgt
     9061 tcgctaatgt tattagtatc gttgattatg taggaatgat gtcagaagtt gacattatcg
     9121 cacaacatgc tgtaactggc gacgaaatgc taagacataa accagtcgac tttgattata
     9181 aagaaaaaac aaatacaatg tcgacaatgc cttattctga gccgttaacc tcttctatgg
     9241 aagattctaa tttagaacca atccgagcaa gagaaaacgc tcaagagcct actgagccta
     9301 aaactaatat tggtgagcgc atcagaggaa tttttggaag tatgtttgac taaaacaata
     9361 agtgaggaat aaaaatggta ttttcatttg atacagcatc agtacaaggt gcagttatta
     9421 aagttattgg tgttggaggc ggtggcggta acgctattaa ccgcatgatt gacgaaggtg
     9481 tcgctggcgt tgagtttatt gcagcaaata ctgatattca agccttaagt agctcaaaag
     9541 ctgaaactgt cattcaactt ggtccaaaat taactcgcgg acttggtgca ggaggacaac
     9601 ctgaagttgg tcgtaaagca gctgaagaaa gtgaagaagt tttaacagaa gcacttactg
     9661 gcgctgatat ggtatttatc acagcaggta tggggggcgg atctggtaca ggtgcggctc
     9721 cagttattgc acgtattgca aagagtttag gagcacttac agtagcggtt atcacacgtc
     9781 cttttggctt tgagggtaat aaacgttcta acttcgctat cgaaggtatt caagaattaa
     9841 gagaacaagt tgatacactg cttatcattt caaataataa tcttcttgaa attgtagata
     9901 agaagacacc attgcttgaa gctcttagtg aagcagataa tgttttacgt caaggtgttc
     9961 aaggtattac agatttgatt actaacccag gtttaattaa ccttgacttt gcagatgtta
    10021 aaacagttat ggcaaataaa ggtaatgcac tgatgggaat tggtatcggt tctggtgagg
    10081 aacgtattac tgaagctgct cgaaaagcaa tttattcacc acttcttgaa acaacgattg
    10141 atggagcaga agacgttatt gtcaatgtta ccggcggtat ggatatgaca cttactgaag
    10201 cagaggaagc atcagagatt gttagccaag cagcaggtaa aggtgtcaat atttggttag
    10261 gcacttcaat tgacatggat atgaaagacg aaatccgcgt aacagttgtt gcaacaggtg
    10321 tgcgtaaaga taaaactaat caagtatctg gttttacaac atcagctcca actaatcaag
    10381 caccttcaga acgtcaaagt acttcaaatt caaactttga tcgtcgtggc aactttgata
    10441 tgacagagtc tcgcgaaatg ccaactcaac aaaatcagcc tcatgcacaa aatcagcaac
    10501 aaagttctgc ttttggtaat tgggaccttc gtcgtgataa tatttcacgt ccgacagaag
    10561 gtgaacttga tagcaagttg tctatgtcaa cgttttcaga aaatgatgat atggatgatg
    10621 aacttgaaac acctccattc tttaaaaacc gttaattaaa atgatgaatt tacaagaaaa
    10681 taaaacagcg atttttgaca atgttagtaa attagcacta aaagcaggtc gcgctcctga
    10741 atcagttcat atcgtagctg taacaaaata tgttaactgt caaacaacag aagcgcttat
    10801 tagaacaggt gttaatcata tcggtgaaaa tcgtgttgat aaatttcttg aaaaatatca
    10861 agcattaaaa gatgaaaagc ttacatggca tctcattggt agtttacaac gtcgaaaagt
    10921 taaagatgtc attaattatg ttgattattt tcatgcttta gattctgtta agttagcagc
    10981 tgagattcaa aaacgtgctc aaaaaccagt taaatgtttt ctacaagtta atatatcacg
    11041 agaagacagt aagcatggtt ttactattga gcaaatagat gatgcactga acttaatttc
    11101 tcggtacgat aaaattgaac ttatcggcat catgactatg gctcccttaa aagcaactaa
    11161 agaggaaata tcatcaattt ttgaagagac tgagagtctt aggaaaaggc ttcaagctag
    11221 gaacatagaa cgaatgccgt ttacagaatt aagcatgggc atgagtagag attatgatat
    11281 tgctattcaa aatggatcaa catttgtaag gataggaact tcattcttta aataatggag
    11341 gggaatatgg cattaaaaga tagatttgac aaaattattt cttattttga cactgacgat
    11401 gtaagtgaaa atgaagtaca cgaagtacaa gagagaactt cagtgcaaag agattccaga
    11461 gcagctacag cacaggaagc ttctcagcgt agtcatatga ctaactccgc agaggaagag
    11521 atgattggta gtcgtccgag aacttctacc tataatccta atcgtcagga gagacaaagg
    11581 gtacagagag acaatgctta tcagcaagct actcctagga ttcaaaataa agactcagtt
    11641 cgacaacaga gagaacaagt aacaattgct ctgaagtatc cccgtaagta tgaggatgca
    11701 caagaaattg ttgatttact tatcgttaat gaatgtgtct tgattgattt tcaatatatg
    11761 cttgatgcgc aagcacgacg ttgcttggat tatattgatg gtgcaagtag agtattatat
    11821 ggttcattac aaaaggttgg gagttcaatg tttctattaa caccagccaa tgttatggtc
    11881 gatattgagg agatgaatat tccaaagact ggtcaagaga cgagttttga ttttgatatg
    11941 aagagacgat aaatatgata ttagtactat taatattatt aaaaacaatt agggtctatt
    12001 cttatatctt actagcctat gccttgttat cttggcttcc gggagcatac gattcatgga
    12061 ttggtagact agtggtacag attgttgatc cgattattcg cccactcaga agatttaatc
    12121 tacaattttt aggtctagat tttacagtaa tagtagcatt ctttttacta aatatgttga
    12181 gtagattttt aatcttgctt tttacataag atgacgttag atgatattta tcagcatttt
    12241 cgaccagaag agtatgcgtt tatcaataaa atagaccatt tagctcaata tgtcgaaaac
    12301 acctattcgt ttattaccac tgaatttcta aatcctaggg aatttaaaat actcgaaagt
    12361 gttctagaga ggcgaggtag tcattattac acttctggtc agtattttca aacagaatat
    12421 gttaaagtaa taatagcacc ggaatactat cagttagata tggttgattt taaccttagc
    12481 ctaattgaaa taaagtataa tgctaaattt aatcacctta cccatgctaa aattatggga
    12541 actttactga attacttagg tgtaaaacga tcaattttag gggatatcct agttgaagaa
    12601 ggctgtgctc aggttttggt cgatagtcaa atgacaaatc accttgttca ttcagttaca
    12661 aaaattggaa ctgctagtgt acaattagct gaagttccct tgtccaaact tctaactccc
    12721 aaacaagata ttcaaaaact aactgttatt gcttccagtt tacgtcttga taaaatttta
    12781 gcaacgattt taaagatttc acgaacacag tcgacgaaat tgattgaggc agataaagtc
    12841 aaggtcaatt atgcaatcgt caatcgagtt tctgaacaat tagtagaggg ggatttgatt
    12901 agtgttagag gatatggtcg atttacgttg aaccataacc tagggttaac taaaaatcaa
    12961 aaatataagt tagaagtaga caaaatgata cataactaag gagaaaacat gccacttaca
    13021 gcacttgaaa ttaaagataa aacattttca tcaaaatttc gcggttatag cgaagaagaa
    13081 gttaatgaat ttttagagat tgttgttgac gattacgaag acttgattag acgtaatcgt
    13141 gagcaagagc aatacattaa agatttagaa gagaaaatcg cttacttcaa cgaaatgaag
    13201 gaatcgttaa gtcaatcagt tattttagct caagaaactg ctgaacgtgt gaaaatttca
    13261 gcacaggatg aagcatctaa cctaatgggg aaagctacat ttgatgctca acatttaatt
    13321 gatgaggcta aattaaaagc aaatcaaatc cttcgtgatg cgacagatga tgctaagcgt
    13381 gtcgctatag aaacagaaga tttgaaacgt caatcacgtg ttttccacca acgcttactt
    13441 tctgaattag aaggtcagtt aaaattagct aactcatctg cttgggaaga attgttgaaa
    13501 ccaacagcta tttatcttca aaattctgat gcttctttca aagaagttgt tgaaaaagtt
    13561 cttgatgaag atgatgcttt accagtggtg gatgacactg agtcatttga tgctactcgt
    13621 caattctcac cagatgaaat ggaagaatta cagcgtcgtg ttgaagaaag taacaaacaa
    13681 cttgaagagt caggtttatt agatactaat aatttccaaa tggaagaacc gatcaattta
    13741 ggtgaaactc aaacttttaa attaaatatt gaagattaag aaaaacaagg ttttaatatt
    13801 atattatcag cgagtaggag atggtgagag ccctacaatc cctaatattg aaattatcat
    13861 tttcaatcat tctacctgaa ctattattta agtaaggtag aaggcttaac ccaccttacg
    13921 ggttgttgag ggagtaagaa atagctattt tctgctctga attaaggtgg taccacgagc
    13981 tttcgtcctt tatgtgatga aagctttttt atttagcaat ttaaaaaatt gtgataacta
    14041 ttactatgag ataaggagtt agtatgaaat taaaagaaac cttgaattta ggacaaacag
    14101 cttttccaat gcgtgcaggg cttccaaata aggaacctca atggcaagaa gcatgggatc
    14161 aagctgatat ttataaaaaa cgtcaagcat tgaatgaagg aaaaccagcc tttcaccttc
    14221 atgacggacc tccctatgct aatgggaata ttcatgtagg acatgcacta aataaaattt
    14281 ctaaagacat tattgtacgt tcaaaatcaa tgtcaggttt tcgagctcct tatgtgcctg
    14341 gttgggatac tcatggactc ccaattgagc aagtattagc taaaaaaggg gttaaacgta
    14401 aagaaatgga cttggctgaa taccttgaaa tgtgtcgtga ttatgctctc agccaagttg
    14461 acaaacaacg tgatgatttt aaacgtctgg gcgtttctgc tgattgggaa aatccttata
    14521 ttacactgac accagattat gaagcagatc aagtacgtgt tttcggtgct atggcagata
    14581 aaggatatat ctatcgtggt gctaaaccag tgtattggtc atggtcatca gagtctgccc
    14641 ttgctgaggc tgaaatcgaa tatcatgata ttgattcgac atcactctac tatgccaata
    14701 aagttaaaga tggtaaggga attcttgata cagataccta tatcgtcgtt tggacgacaa
    14761 caccatttac tgtaacagct tcacgcggtt taacagtagg accagatatg gagtatgttg
    14821 tagttgcacc agtaggtagt gagcgtaaat accttcttgc agaggttctt gtagatagtc
    14881 tcgctgctaa gtttggctgg gaaaactttg aaattgtgac tcatcacact ggtaaagaac
    14941 ttaatcacat tgttacagaa catccatggg atacagaagt agaagagttg gttatccttg
    15001 gagaccatgt tacaacagat tctggtacag gtattgtcca cacggctcct ggttttggtg
    15061 aagatgacta taacgttggt attgctaatg gacttgatgt tgtagttacc gtagatagtc
    15121 gtggtttgat gatggaaaat gctggtcctg attttgaagg tcaattctat gacaaagtga
    15181 caccacttgt aaaagaaaaa ttgggagatc ttcttttagc cagcgaagtt atcaaccact
    15241 catatccatt tgactggcgt acgaaaaagc caatcatttg gcgtgcggtt ccacaatggt
    15301 ttgcctctgt ttcgaaattc cgtcaagaaa tcctcgatga aattgaaaag acaaatttcc
    15361 aacctgagtg gggtaaaaaa cgtctttata acatgatccg tgaccgtggc gactgggtaa
    15421 tctcacgtca acgtgcttgg ggtgttccac ttccaatttt ttatgcagaa gatggtacag
    15481 ctatcatgac aaaagaagtg actgatcacg ttgcagattt atttgctgaa tacggctcaa
    15541 tcgtttggtg gcaacgtgat gctaaggacc ttctaccagc aggttatact catccaggtt
    15601 cacctaatgg tctctttgag aaagaaacag acatcatgga tgtttggttt gattctggtt
    15661 catcatggaa tggagtaatg aatgctcgtg aaaacctttc ctatccagca gatctttatc
    15721 ttgaaggttc tgatcaatac cgtggttggt tcaactcatc actaattaca tcagttgctg
    15781 ttaatggtca cgctccatac aaagcagtct tatctcaagg ttttgtactt gatggtaaag
    15841 gtgagaaaat gtctaaatct ctaggaaata caattcttcc gagtgatgtt gaaaaacaat
    15901 ttggtgcgga aatcttgcgt ctgtgggtaa cttctgttga ttcaagtaat gatgttcgta
    15961 tttcaatgga tatcttaaaa caaacatcag aaacttatcg taagatcaga aatacattgc
    16021 gtttcttgat tgcaaatact tccgatttta atcctaaaca agatgcagta gcctatgaaa
    16081 atttaggtgc cgttgatcgt tatatgacta ttaaatttaa tcaagttgtg gatacaatca
    16141 acaaagctta tgctgcttat gattttatgg caatttataa agctgtcgtt aacttcgtaa
    16201 cagttgatct atcagcattt taccttgatt ttgcaaaaga tgtggtttac attgaagcgg
    16261 ctaacagtcc agaacgacgt cgtatgcaaa ctgttttcta cgatatttta gttaaactta
    16321 caaaactttt gactccgatt ctaccacata ctgctgaaga aatttggtca taccttgaac
    16381 atgaggagga ggaatttgtt caattagcag aaatgcctgt tgcacagaca ttctctggtc
    16441 aagaagaaat tcttgaagag tggtcagcat tcatgacgtt acgaacacaa gctcaaaaag
    16501 ctctcgaaga agcacgtaac gctaaagtca ttggtaaatc tctagaagca catttaacaa
    16561 tttatgctag tcaagaagtg aaaacgttat taacagcgtt aaatagcgat attgcactct
    16621 tgatgattgt ctcacaatta acaattgctg atgaagcaga caaaccagct gactcagtat
    16681 catttgaagg tgttgcattt actgttgaac acgctgaagg tgaagtctgc gaacgctcac
    16741 gccgtattga tccaaccaca aggatgcgtt cgtatggtgt agctgtttgt gatgctagtg
    16801 cagcaattat tgagcaatat tatcctgaag cagtagctca aggttttgaa gcttaataaa
    16861 aattatttaa tgctatattt gtttagtagt catcaaaaaa gaagccttga ttaggcttct
    16921 tttaattatg ctgttgttgg atatgaaatt tcaattaact tggcagtatg gagtgatttc
    16981 atctgctctt gaataataat agattgatct attttacgct ttaagaagaa ctcacgaata
    17041 gcttctaatt cttcatcttt gatagcacga tatttgtttg aaattaataa ttcataatgt
    17101 ttaggtgctg ttgtaaaaac gacatcggtg ttgccagctg aatagacttc cactaatttt
    17161 gcatctgtat tttggagttg atttcgaact agttcaggat gactactggt tgtattaatt
    17221 agtctcataa ctattttccc cctgattctt attaggttta ttataacact atttaggaag
    17281 aaaggtatga attatttcct agaatggtga tttttttccc aagcttggac accccatcta
    17341 tggcttcctc gttttagctt ttctttagct tcttggatag gaaaccaggc tagatggttg
    17401 aaatcttcta gtggcgcttg ctccttatga taggcagtaa cttcataaat ataggcggga
    17461 ttatagtagt aagtatcacg gtgtctagag taaaaatatt catcagcttg accatagtaa
    17521 tggccaattg ttgcagaata acctaattct tcaattaact cacgagttaa agcttctagg
    17581 tgattttcat tttcttcaat ttccccacct ggaagaaacc aagcaccatt aggtgcctgt
    17641 actaaaataa ttttatcatg agttggattt ggaataatag catagacacc aaatcgtgac
    17701 ctatagtcta cattatctat tttttcaccg aaag
//
LOCUS       DATROQ010000039        15566 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.039, whole genome shotgun sequence.
ACCESSION   DATROQ010000039 DATROQ010000000
VERSION     DATROQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15566
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.039"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            146..571
                     /gene="rplK"
                     /locus_tag="VBC94_001143"
     CDS             146..571
                     /gene="rplK"
                     /locus_tag="VBC94_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEO6979418.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGEPNKTKVATITR
                     AQVQEIAETKMPDLNAANLESAMRMIEGTARSMGFTVTD"
     gene            675..1364
                     /gene="rplA"
                     /locus_tag="VBC94_001144"
     CDS             675..1364
                     /gene="rplA"
                     /locus_tag="VBC94_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEO6979419.1"
                     /translation="MAKKSKNLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     SYNLNIDVKKADQQIRGAMVLPAGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KIQGGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDDAKLVDNFKAFNDVIVKAKPATAKGTYITNLSITTTQ
                     GVGIKVDPNSL"
     gene            1753..3369
                     /gene="nikA"
                     /locus_tag="VBC94_001145"
     CDS             1753..3369
                     /gene="nikA"
                     /locus_tag="VBC94_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HEO6979420.1"
                     /translation="MRRNILLSITCLLMVTLTACHSQDSKSHKLNSDKLTLAWGEDFG
                     DVNPHRYNPDQFVIQDMVYEGLVRYGDNGKIEPALAKSWSISQDGKTYTFKLRNAKYS
                     DGSNFNAANVKRNFDSIFSKSNRGNHNWFNLTNQLENYRALNQSTFEIKLKQAYSATL
                     YDLSMIRPIRFLSDSAFPKGDDTTKKNVKKPIGTGQWVVKSKKQNEYITFKRNENYWG
                     KKPKLKEVTVKVIPDAQTRALAFESGDVDLIYGNGIIGLDTFAQYTKDKKYVTAISQP
                     MSTRLLLLNAKESIFQDKKVRQAMNHAIDKVSIAKNTFRGTEKPADTIFSKSTSHSDA
                     KLNPYSYNVDKANQLLDQAGWKMGKDKVREKDGKALTLRLPYIATKATDKDLVTYFQG
                     EWRKIGINVSLIAMEEDDYWANAKKGNFDMMLTYSWGAPWDPHAWMSALTAKADHGHP
                     ENIALENLATKTEMDRLIKSALVDPKEENVDRDYKKVLELLHDEAVYIPLTYQSVISV
                     YRKGDFKTMRFAPEENSFPLRYIEKNNVSK"
     gene            3356..4300
                     /locus_tag="VBC94_001146"
     CDS             3356..4300
                     /locus_tag="VBC94_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6979421.1"
                     /translation="MYLSSSIIKKILSAFLALFFISLLTFILIKLSTVNPAENYLRLS
                     KISVSPEALKEAEHYLGLDKPLWKQYWLWFQKALTGDFGYSYVLRLPVLDLVLQRFLA
                     TLFLGTSAFLLIVTISTPLGVWAGLHESARSDHLIRFLSFSSVSMPNFWVAYLLMLLF
                     SAKLNLLPVSGGNDLQSLILPSITLSFSTVGQYIALIRKAISQENRSLNVENARLRGV
                     KERYIVTHHLLRNALPAIMTALSLTWVYLLTGSIIVEEIFSWNGIGRLFVTSLRTSDL
                     PVIQACMLIFGTLFLANNFMTQCFMNWVDPRLRKSREK"
     gene            4300..5106
                     /locus_tag="VBC94_001147"
     CDS             4300..5106
                     /locus_tag="VBC94_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6979422.1"
                     /translation="MSKRIYFSSSLLVLLVISAIFAPILSSFDPQYVDLSQKLLAPNN
                     VHLLGTDQLGRDVLSRLLYGARYSLFLAIIISLLELTIGMFVGLIVGWYQGKLENLFL
                     WIANIILAFPSFLLSLATVGILGHGLGNLIFAIVFVEWVYYAKLMTNLVKSAKKEPYV
                     INAQIMGLSVWHILRKHIFPFVYQPILVMVLMNIGNIILMISGFSFLGIGVQPNVTEW
                     GMMLHDARGYFRTATWMMLSPGIAIFLTVFSFNTLGDAIDKKDWKRQWNS"
     gene            5094..5882
                     /locus_tag="VBC94_001148"
     CDS             5094..5882
                     /locus_tag="VBC94_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979423.1"
                     /translation="MEQLEIRKLSLQIGEVPVLRDFSCKIDMGESLTIIGESGSGKTL
                     LAKLLVGHIPQGMTVRGNIFFKGVDLGKLTVKQWQKLRGRDIAYLVQNPMSMFNPFQK
                     IEAHILETILSHEKCSKRVALSKALEWMKRLNLDDAISLLKKSPFELSGGMLQRIMLA
                     TILSLDPQVIILDEPTSAVDCHNCSTISAILQELQNNGKTLITVTHDYQLARDLGGQL
                     LVISEGEVVEQGQTQAILSNPQHNYTKALTVQMEYEGDILNVGL"
     gene            5869..6549
                     /locus_tag="VBC94_001149"
     CDS             5869..6549
                     /locus_tag="VBC94_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979424.1"
                     /translation="MLVCNHVGKTFGRQEVLKDCHFHLKRGEIIGIMGKSGSGKSSLA
                     RLIIGLDSPTCGSIYFQGKIYTPKDGKAQIILVFQDALSSVNPYFSIEEILNEAFYGK
                     KTTFELCQILEAVGLDGTYLKYKARQLSGGQLQRVCIARALLLKPKIIIFDESLSGLD
                     PVTQIKMLRLLQKIKRRYELSFIMISHDPKICQAICNRVFLIKNGYLVEDNEFLKRAC
                     STNCLTNL"
     gene            6670..7398
                     /locus_tag="VBC94_001150"
     CDS             6670..7398
                     /locus_tag="VBC94_001150"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEO6979425.1"
                     /translation="MEPKYQRILIKLSGEALAGDKGVGIDIPTVQSIAKEIAEVHNSG
                     VQIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQYGVDTRVQT
                     AIPMQTVAEPYVRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEAEAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHVEVIKRGLKIMDATASTISMDNDIDLVVFNMN
                     ETGNIKRVVLGEQIGTTVSNKASE"
     gene            7414..7971
                     /gene="frr"
                     /locus_tag="VBC94_001151"
     CDS             7414..7971
                     /gene="frr"
                     /locus_tag="VBC94_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEO6979426.1"
                     /translation="MTKEIVTKAQERFEQSHQSLSREFAGIRAGRANASLLDRIQVEY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERAINESDLGINPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKIAIRNIRRDAMDEAKKQEKNKEITEDDLKSLEKDI
                     QKATDDAVKHIDEMTANKEKELLEV"
     gene            8089..8943
                     /gene="cvfB"
                     /locus_tag="VBC94_001152"
     CDS             8089..8943
                     /gene="cvfB"
                     /locus_tag="VBC94_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEO6979427.1"
                     /translation="MNTLLATVITGLVTDENKDFYFIQKDGFTFALSKSEGEHHIGEM
                     VKGFAYTDMQQKARLTTKETFATRDHYGWGTVTEVRKDLGVFLDTGLPDKQVVVSLDV
                     LPELKELWPKKGDRLYVCLDVDKKDRLWALPADPEVFQRMATPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYSEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKKIKQ
                     DQLGTELL"
     gene            9081..9590
                     /gene="msrA"
                     /locus_tag="VBC94_001153"
     CDS             9081..9590
                     /gene="msrA"
                     /locus_tag="VBC94_001153"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEO6979428.1"
                     /translation="MERAIFAGGCFWCMVQPFEELDGIESVLSGYTGGHVENPTYKEV
                     CSKTTGHTEAVEIIFNPEKISYADLVELYWAQTDPTDAFGQFEDRGDNYRPVIFYENE
                     EQRQIAQKSKDKLQASGRFDRPIVTSIEPADTFYPAEDYHQAFYRTNPARYALSSARR
                     HAFLEENWH"
     gene            9587..9802
                     /locus_tag="VBC94_001154"
     CDS             9587..9802
                     /locus_tag="VBC94_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEO6979429.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADYAFDETTFPKHSSDFETV
                     SRYLEDEASFSFNLTDFDDIWEDYLNH"
     gene            9959..11731
                     /locus_tag="VBC94_001155"
     CDS             9959..11731
                     /locus_tag="VBC94_001155"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEO6979430.1"
                     /translation="MRYTNGNFEAFARPRKPEGVDKKSAYIVGSGLAGLAAAVFLIRD
                     GQMDGQRIHIFEELPLSGGSLDGVKRPDIGFVTRGGREMENHFECMWDMYRSIPSLEV
                     PDASYLDEFYWLDKDDPNSSNCRLIHKQGNRLESDGDFTLGTHSKELVKLVMETEESL
                     GAKTIEEVFSKEFFESNFWTYWATMFAFEKWHSAIEMRRYAMRFIHHIGGLPDFTSLK
                     FNKYNQYDSMVKPIISYLESHNVDVQFDSKVTNISVDFKNGQKLAKAIHLTVGGEAKT
                     IDLTPNDFVFVTNGSITESTNYGSHDTVAKPNTDLGGSWNLWENLAAQSDEFGHPKVF
                     YKDIPKESWFVSATATIKDPAIEPYIERLTHRDLHDGKVNTGGIVTVTDSNWMMSFAI
                     HRQPHFKEQKENETIVWIYGLYSNVEGNYIKKPIEECTGREITEEWLYHLGVPEMKIH
                     DLSDKQYVSTVPVYMPYITSYFMPRVKGDRPDVIPQGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYTFLNIERGVPEVFNSAFDIRVLLQSLYYLNDKKSVEDMDLPIPAL
                     MRKVGMKKIRGTYLEELLREAHLL"
     gene            11855..12841
                     /locus_tag="VBC94_001156"
     CDS             11855..12841
                     /locus_tag="VBC94_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEO6979431.1"
                     /translation="MQEYSIEITLQHPDDMMSLFGSNERHLKLIEENLDVIIHARTER
                     VQVLGDSEEAVETARLTIEALLVLVNRGMTVNTSDVVTALSMAQNGSIDKFVALYEEE
                     IIKDSYGKPIRVKTLGQNIYVDSVKNHDVVFGIGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKEQTSRLMEREIIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDVSQIDLPKNVKS
                     GLIDAVEKLRNIKKIDFIHLSAKDVVRHPVVAEIINAYSDSESSHKLQSDKD"
     gene            complement(12932..13735)
                     /locus_tag="VBC94_001157"
     CDS             complement(12932..13735)
                     /locus_tag="VBC94_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HEO6979432.1"
                     /translation="MDHFTKLWQDFSKLPNVVAIALGGSRSGDSFDQSSDYDLYVYCA
                     ATPDITSRKRILNKHCHYIELNNHYWELEDNGTLNDGTDIDILYRNIDNFLSDLEDVV
                     EHHNSRIGYTTCFWHNLINCQILYDPENQLQSLKERFEVSYPSQLQKQIIIQNRNLLT
                     GKLPSYDKQIIKALKRQDFVSTHHRTTAFLDSYFDIIFALNKLTHPGEKRMISYAKKN
                     ATLLPKHFEENIIKLCHHNSNEHTVKETLNDIIMHLDVMLKENFQHFIG"
     gene            13927..14403
                     /locus_tag="VBC94_001158"
     CDS             13927..14403
                     /locus_tag="VBC94_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO6979433.1"
                     /translation="MKQDYISYIRSKVGHETIFLTYSGGILTDGKGRVLLQLRADKNS
                     WGIIGGRMELGESSVDTLKREFFEETGLRVEPIRLLNVYTNFQDSYPNGDKAQTVGFI
                     YEVSCPKPVNIEGFHNEETLQLDYFSKEDVKNITIVNEQHQLILDEYFSQTFQMGR"
     gene            14725..15531
                     /locus_tag="VBC94_001159"
     CDS             14725..15531
                     /locus_tag="VBC94_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6979434.1"
                     /translation="MKITLHGVAETLLITLYIRAKDAMAKHPILNDQKSLAIVEQIEY
                     DFDKFDNSEASFYATLARIRVMDREIKKFIRENPNSQILSIGCGLDTRFERVDNGQIR
                     WYNLDLPEVMEIRKLFFEEHERVTNIAKSALDETWTREVNPQNAPFLIVSEGVLMFLK
                     EDDVETFLHILTNSFSQFMAQFDLCHKEMINKGKQHDTVKYMDTEFQFGITDGHEIVD
                     LDPKLKQINLINFTDEMSKFELGTLRSLLPTIRKFNNCLGVYEYKASEKK"
BASE COUNT         5182 a         2327 c         3089 g         4968 t
ORIGIN      
        1 aatttaaaat ataatgaaat taatggctta aaactattgc ttttatttat gattatagta
       61 taatggtaag gtatgttata aacatgcctg tgggaggtaa aaatttctaa ttaccgccaa
      121 aaccacaaca ggaggatttt taaaaatggc taaaaaagtt gaaaaacttg taaaacttca
      181 aattcctgct ggtaaagcaa ctccagctcc accagttgga ccagcacttg gtcaagcagg
      241 aatcaacatc atgggattca ctaaagagtt taacgctcgt acagctgatc aagctggtat
      301 gattattcca gttgttatct cagtatatga agacaaatca tttgatttca tcactaaaac
      361 accaccagct gctgttcttt tgaaaaaagc tgcaggtgtt gaaaaaggat caggtgaacc
      421 taacaaaact aaagttgcaa caattactcg tgcgcaagta caagaaattg ctgaaactaa
      481 aatgccagat ttaaacgctg caaaccttga gtctgcaatg cgtatgatcg aaggtactgc
      541 tcgttctatg ggattcactg ttactgacta atcagaaaca cccacataac ccgcaagact
      601 tcatcgtagt ttgatgagtg acgtgggaga ttttaaatca aatcgattga ccacattaca
      661 aggagaattt taaaatggct aaaaaaagta aaaacttacg tgctgctctt gagaaaattg
      721 acagcactaa agcatacagc gtagaagaag ctgtagctct tgcaaaagaa actaactttg
      781 ctaaatttga tgcaactgtt gaagtttctt ataaccttaa cattgacgtt aaaaaagctg
      841 accaacaaat ccgtggtgct atggttttac cagcaggtac tggtaaaact tcacgtgttc
      901 ttgtttttgc tcgtggtgct aaagctgaag aagctaaagc agcaggtgca gactttgtcg
      961 gtgaagatga cttagttgct aaaatccaag gtggatggct tgactttgat gttgttatcg
     1021 caacaccaga tatgatggca cttgttggac gtcttggacg tgtccttgga cctcgtaact
     1081 tgatgccaaa ccctaaaact ggtacagtaa ctatggacgt tgctaaagca gttgaagagt
     1141 ctaaaggtgg taaaattact taccgtgctg ataaagcagg taacgtccaa gcccttattg
     1201 gtaaagtttc atttgatgat gctaaattag ttgataactt caaagcattt aatgatgtta
     1261 ttgttaaagc taaaccagct actgcaaaag gaacttacat cacaaacctt tcaattacaa
     1321 caacacaagg tgttggtatc aaagttgatc ctaactcact ttaatcaata aaaaactagg
     1381 aattccttcc tagttttatt tattctatat acttaactag ttaagtatat attttaataa
     1441 tcaaaggata ttatatgtgt ttcttttgaa aaatagcttt ttatctgtat taaccttctt
     1501 aaagactaat gaatgtgcga tgtgttttaa gtaacagatg agaataagga aaaatgaaat
     1561 tggcgatatg gtatgtgact attatgatta ttctttagca ataatctatt aaaatgagtt
     1621 cgcagggaat aagtaaattt tgtatcgttg tagatttttt attttaagat tcttgacaag
     1681 acattactaa aatgatttaa ttaagtttgc ttaaaaaaat aattaactaa acttaaaaga
     1741 aagttggtaa aaatgagacg taacatatta ttgagcatta cttgtttatt gatggtgact
     1801 ctgactgcat gccattcaca ggatagcaaa agtcataaac taaacagtga taaactcacg
     1861 cttgcttggg gagaagactt tggtgatgtt aatccgcatc gctataaccc tgatcagttt
     1921 gttatccagg atatggttta tgaggggttg gtacgatatg gtgacaatgg gaaaattgaa
     1981 ccagctttag cgaaaagctg gagcattagt caagatggaa aaacctacac ttttaaattg
     2041 agaaatgcta aatattctga tggcagtaac tttaatgctg cgaatgtcaa acgtaatttt
     2101 gatagtattt tttcgaagtc taatagaggt aatcataatt ggtttaattt aacgaatcaa
     2161 ttggaaaatt atcgtgcctt gaatcaaagt acttttgaaa ttaaactgaa acaagcttat
     2221 agtgctactt tatatgatct ctctatgatt cgaccaattc gctttttatc tgatagcgct
     2281 ttccctaaag gtgatgatac aactaagaag aatgtaaaaa aaccaattgg aacgggtcaa
     2341 tgggttgtga aaagtaaaaa gcaaaatgaa tatattacct tcaaacgtaa tgagaattac
     2401 tggggaaaga aaccaaaatt aaaagaagta accgtaaaag ttatacccga tgctcagacg
     2461 cgtgctttag cattcgaatc aggtgacgtt gatttgatct atgggaatgg cattattggt
     2521 ttagacactt ttgcccaata tacgaaggac aagaaatatg tcacagctat ttctcaacct
     2581 atgtcaacaa gacttttgtt gctaaatgct aaggaaagta ttttccaaga taaaaaagtt
     2641 cgtcaggcaa tgaatcatgc tattgacaag gtttctattg ctaagaacac ctttagagga
     2701 acagaaaagc cagcagacac tattttttca aaatcaacat ctcactcaga tgctaagtta
     2761 aatccttata gctataatgt tgataaagca aatcagctat tggatcaagc tggttggaag
     2821 atgggaaaag ataaggttcg tgaaaaagat ggtaaagctc taaccttacg tcttccttat
     2881 atcgccacta aggcaactga taaagacctg gtaacttatt tccaaggaga atggcgtaaa
     2941 atcggtatta atgtttctct tattgctatg gaagaagatg attattgggc taatgctaaa
     3001 aagggtaatt ttgatatgat gttaacctat tcttggggag caccatggga ccctcatgct
     3061 tggatgtcgg cattaactgc caaggcagat catggacatc cagaaaatat agctttagaa
     3121 aatctagcaa ctaaaactga aatggataga cttattaagt cagctctagt agatcctaag
     3181 gaagaaaatg ttgatagaga ctataagaag gttcttgaat tattgcatga tgaggctgta
     3241 tatatcccat taacttatca atcggtgatt tctgtttata ggaaaggtga ttttaaaacc
     3301 atgcgttttg cgccagaaga aaattcattc ccattacgct atatagaaaa aaacaatgta
     3361 tctaagtagt tcgatcatca aaaaaatatt gtcagctttt ttggcattgt ttttcatatc
     3421 acttctaaca tttattctta tcaaactatc aacagttaat ccagcagaaa attaccttcg
     3481 tttatcaaaa ataagtgtta gtccagaagc gctaaaagaa gcagagcact atttaggttt
     3541 agataagcca ttatggaagc agtactggct gtggttccaa aaagcattaa caggagattt
     3601 tggatattct tatgtgctca ggttgccagt attagatttg gttttacaac ggtttttggc
     3661 tactttattt ttaggcacga gtgccttctt gctaattgtt accatttcta ccccgttagg
     3721 tgtgtgggct ggcttacatg aatctgctcg gagtgatcac ttgattcgat ttttgagttt
     3781 ttctagtgtg tctatgccaa atttttgggt tgcttacttg ttgatgctgc tattttcggc
     3841 aaaattaaat cttctgcctg tttcaggagg taacgattta caaagtttaa ttttgccaag
     3901 tattacctta agtttttcta ccgtaggtca atacatagcc cttattcgaa aagctattag
     3961 tcaagaaaat agaagtttaa atgtggaaaa tgcccgatta agaggagtaa aagaacgtta
     4021 tattgtcaca catcacctcc ttagaaatgc cttacctgcg ataatgacag cacttagctt
     4081 gacttgggtt tacttattaa caggatcaat tattgttgaa gaaatttttt catggaatgg
     4141 aataggacgt ttatttgtga ccagtctaag aacttcagat cttccagtaa tacaagcttg
     4201 tatgctaatt tttggaacct tatttttagc taataatttt atgacacagt gttttatgaa
     4261 ttgggttgat cctcggttac gaaagtcaag ggagaaataa tatcaaaaag aatttatttt
     4321 tcgagtagct tactggtatt gttagtgatt agtgccattt ttgcccctat tctatctagc
     4381 tttgatccac aatacgtaga tttatcacaa aaattattgg ctcccaataa tgttcatttg
     4441 ttagggactg accaattggg tagagatgta ttatctcgct tgctttatgg tgctagatat
     4501 tcactgtttt tagctattat tattagctta ttggaattaa cgattggtat gtttgtgggt
     4561 cttattgttg gttggtatca aggaaagtta gaaaacctct ttttatggat agctaatatt
     4621 attttagctt ttccgagctt tttgctctct cttgcaaccg tggggatctt aggtcatggt
     4681 ctagggaatt taatttttgc aattgtcttt gtggaatggg tttactatgc taaattaatg
     4741 accaatttag ttaagagtgc aaaaaaagag ccttatgtga taaatgcgca aattatggga
     4801 ctttcagtct ggcatatttt aagaaaacat atttttcctt ttgtgtatca gccaatcctc
     4861 gttatggtcc ttatgaatat aggaaatatt attttaatga tttctggctt ttctttctta
     4921 gggattggtg tgcaacctaa tgtcacagaa tggggaatga tgttgcacga tgctagagga
     4981 tattttagga cagctacttg gatgatgtta tctcctggaa ttgcgatttt tttaacagta
     5041 ttttccttta atactttagg cgatgccata gataaaaaag attggaaacg acaatggaac
     5101 agttagaaat aagaaaattg tcattacaga taggagaggt ccctgtacta agagatttta
     5161 gttgtaaaat agatatggga gaatcgctaa ctattattgg tgagagtggt tcagggaaaa
     5221 ccctcttggc aaaattattg gttggtcaca ttccacaagg tatgacagtt agaggaaaca
     5281 tattttttaa gggagttgat ttaggtaaac taactgtaaa gcagtggcag aaattaaggg
     5341 gacgagatat tgcctattta gtgcaaaatc ccatgtctat gtttaatcct tttcaaaaaa
     5401 ttgaagcgca tattttggaa acaatcctta gtcatgagaa atgttcaaag agagtagcct
     5461 tatctaaagc gttagaatgg atgaaacgtt taaatttaga tgatgcgata tccctgttaa
     5521 aaaaatcccc ttttgagctt agtggaggaa tgctacaaag aattatgtta gccactatat
     5581 tatctcttga tcctcaggta atcatattag atgagccgac ctcagcggtt gattgtcata
     5641 attgttctac tatatcagct attttgcagg aattgcaaaa taatggaaaa accttgatta
     5701 ctgtaacgca tgattatcag ttagctagag acctgggggg acaattatta gttataagtg
     5761 agggagaagt tgttgaacag ggacaaaccc aagctatttt aagcaatcct caacataact
     5821 atacgaaagc actgacagtg caaatggagt atgaaggaga tattcttaat gttggtttgt
     5881 aatcatgtcg gaaaaacatt tggacgacaa gaggttttaa aggactgtca ttttcatctt
     5941 aaaagaggag aaataattgg tataatgggt aaaagtggca gtggtaaaag tagccttgcc
     6001 cgactcatta taggactaga tagtcctact tgtggttcga tatattttca aggaaaaatt
     6061 tacacaccta aagatggtaa ggcgcagatt atccttgttt ttcaagatgc gcttagttcg
     6121 gtcaatccat attttagtat tgaggaaatt ttgaatgaag ctttttatgg aaaaaaaaca
     6181 acttttgaat tatgtcaaat attagaagcg gtgggtttag atggaactta tctaaaatat
     6241 aaagctagac aacttagtgg tggtcagcta cagagagttt gtatcgctag agctttactc
     6301 ttaaagccta aaattattat ttttgatgaa tccttaagtg gattagaccc agtaactcaa
     6361 attaaaatgc tacgtttatt acaaaaaata aaacgccgtt atgaattaag ttttataatg
     6421 atttctcatg accctaaaat ttgtcaagct atctgtaacc gggtctttct gataaaaaat
     6481 ggttatttag tagaagataa tgaattcttg aagagagcgt gttctactaa ctgtttgacc
     6541 aatctctgaa ttattatgtt tttcttattt taggagttga taaagaaatc aacttactag
     6601 tgtcattgct ctattaaaat tttgcctttt atggtaaaat aataaagaca aaataaggag
     6661 aacatagtag tggaacctaa atatcaacgt atattaatta agttatctgg tgaagcattg
     6721 gcgggagata agggtgtcgg tattgacatt cctaccgttc aatctattgc aaaagaaatt
     6781 gccgaagtac acaattcagg tgttcaaata gcacttgtca ttggtggagg taatctttgg
     6841 cgtggagaac ctgcagcaga agcagggatg gatcgtgttc aagctgatta tacagggatg
     6901 ttagggacag taatgaatgc tcttgtaatg gctgatagcc ttcaacaata tggggtagat
     6961 actagagttc aaacagctat tccaatgcaa actgttgctg agccttacgt acgtggtcgt
     7021 gctttacgtc atctagagaa gaatcgtatt gttgtctttg gagcgggtat tggttcgcct
     7081 tatttctcaa ctgatacaac agcagcgctt cgtgctgctg agattgaggc agaagcaatt
     7141 ttaatggcta aaaatggagt agatggtgtt tataatgctg atccaaagaa agatgccaat
     7201 gctgtcaaat ttgatgaatt aactcacgtt gaagtaataa aacgagggtt aaaaatcatg
     7261 gatgcaaccg catcaactat ttcaatggat aatgatattg accttgttgt tttcaatatg
     7321 aatgagactg gtaatattaa gcgtgttgtt cttggagaac aaatcggaac aactgtttca
     7381 aacaaagcat ctgaatagga gtgtataaaa aatatgacta aggaaatcgt tacaaaagct
     7441 caagagcgtt ttgagcaatc acaccaaagt ttatcaagag aatttgcagg gattcgtgca
     7501 ggacgtgcta atgctagctt gcttgatcgt attcaagttg agtattatgg agcgccaacc
     7561 ccacttaacc aattagcttc tattactgta cctgaagctc gtgttctttt aatttcacca
     7621 tttgataaat catcaatcaa agatattgag cgtgctatta acgaatcaga tttaggtatt
     7681 aatcctgcaa atgatggttc agttattcgt cttgttattc cagctttgac agaagaaaca
     7741 cgtcgtgatt tagctaaaga agtgaaaaaa gtcggagaaa atgctaagat tgctatccgt
     7801 aatattcgcc gtgatgctat ggatgaagct aagaagcaag aaaagaataa agaaattaca
     7861 gaagatgact tgaaatctct tgaaaaagat attcaaaagg ctactgatga tgctgtaaaa
     7921 catattgatg agatgactgc taataaagaa aaagaattgt tggaagttta atagtaacgt
     7981 ttgctatctt tattatgatt ggcaagtaat aaaatgatta tggaggtaac tgatagaatc
     8041 aaaaccagat ggatttggtt tgtcggttac ctttttaaaa ggagaaaaat gaatacatta
     8101 ttagcgacag tcataacagg cttagtaact gatgaaaaca aagattttta ctttatccaa
     8161 aaagatggct ttacctttgc attatcaaag tcagaaggag agcatcacat tggtgaaatg
     8221 gttaaaggat tcgcctatac agatatgcaa caaaaagcac gtcttacaac taaagaaaca
     8281 tttgcgactc gagatcatta tggttggggg acagttactg aggtacgtaa agacttggga
     8341 gtatttcttg atactggtct tccagataag caagtggttg tttctttgga tgttcttcct
     8401 gaattgaaag aactttggcc taaaaaaggt gatcgcctct acgtttgctt agatgttgat
     8461 aaaaaagatc gtctctgggc tctccctgca gatcctgaag tgtttcaacg aatggcaacc
     8521 cctgcatata acaatatgca aaatcaaaat tggccagcaa tcgtttatcg cttaaaattg
     8581 tcaggaactt ttgtttatct accagaaaat aatatgctcg gctttattca ccctagtgaa
     8641 cgttacagtg aacctcgttt agggcaagtg ctggatgcac gagttattgg cttcagagaa
     8701 gttgatagaa ccttaaattt atcattaaag cctcgttctt ttgaaatgtt agaaaatgac
     8761 gcacaaatga ttttaacgta tttagaaagt aacggaggat tcatgacttt aaatgataaa
     8821 tcttctccag aggaaattaa agctactttt ggaatttcca aaggtcagtt taaaaaagca
     8881 cttggaggac tcatgaaagc taaaaagatc aaacaagatc agctaggtac agaattatta
     8941 taaacttttg aacttttaaa gttttctcac gaggtcgttt tagttatagc tagaattaat
     9001 ggctaattat agaaaaaagc tgagaggaaa ttttggcttt ttttgttata atgtatctat
     9061 taaaaagaat ggagaacgat atggaacgtg cgatttttgc tggaggttgt ttttggtgta
     9121 tggtacaacc atttgaagaa ttagatggta ttgaatctgt tttatctgga tacactggag
     9181 gacatgttga gaatccaact tataaagaag tttgtagtaa aactactgga catacagaag
     9241 ctgttgaaat tatttttaat cctgaaaaaa tctcttatgc agatttagtt gagttatatt
     9301 gggcacaaac agatccaaca gacgcttttg gtcagtttga agatcgtggg gataattatc
     9361 gtcctgtcat cttttatgag aatgaagagc aacgtcagat tgctcaaaaa tcaaaagata
     9421 agctacaagc ttcgggtaga tttgatagac caattgtaac cagcattgag cctgctgata
     9481 ctttttaccc agcagaagac tatcatcaag cattctatcg gactaaccct gctcgttacg
     9541 ccttgagtag tgccagaaga catgcatttc tagaggagaa ctggcattga gaaaatcatt
     9601 ttacagttgg ttgatgacac aaagaaatcc aaaatcaaat gaaccagtag caatcctagc
     9661 agattacgct tttgatgaaa ccacttttcc aaaacatagt tcggattttg aaacagttag
     9721 tcgctattta gaagatgagg ccagtttttc ctttaattta acagattttg atgatatttg
     9781 ggaagattat ctcaatcatt aacaataata tagagattgt attttcaatc tcttttttag
     9841 gttatagaaa ttggacacat tgtctaagac tgtctgaaaa gcaggttttt taatggcaat
     9901 cgttttcaca aaggagtaga atatagtcat ctaaaatata gccctaggga ggttgtttat
     9961 gagatataca aatggaaatt ttgaagcctt tgcaagacct cgaaaacctg aaggtgtgga
    10021 taaaaaatcc gcttatattg ttggttctgg tttagcagga ttagctgccg ctgtcttttt
    10081 aatacgtgac ggtcaaatgg atggtcaacg tattcatatt tttgaagaac tacctctttc
    10141 tggaggatca cttgacggtg tcaaacgacc tgatatcggt tttgtaacgc gtggtggtcg
    10201 tgaaatggaa aatcacttcg aatgtatgtg ggatatgtac cgttccatcc cctctctcga
    10261 agttccagat gcttcttatc tagatgaatt ttattggctt gacaaggatg atcccaattc
    10321 atctaactgt cgcctcattc ataaacaggg gaatcgctta gaatctgatg gtgattttac
    10381 actcggaaca cattccaaag agttagttaa gctagtcatg gagactgaag agtctttagg
    10441 tgctaagacg attgaagaag ttttttcaaa agaatttttt gaaagtaatt tttggactta
    10501 ttgggctact atgtttgcct ttgagaaatg gcattcagcg attgaaatgc gtcgatatgc
    10561 tatgcgcttt atccatcata ttggtggtct gcctgatttc acttcattaa aatttaataa
    10621 atataatcaa tatgattcta tggtgaaacc aatcatcagt tatttagagt ctcataatgt
    10681 agatgttcaa tttgatagca aggtaactaa tatctccgta gactttaaga acggtcagaa
    10741 gcttgcaaaa gccattcatc tcacagttgg tggtgaagca aaaacaattg atcttactcc
    10801 aaatgatttt gtttttgtaa cgaatggttc tatcactgaa agtacgaact atggtagtca
    10861 tgatactgta gctaaaccta ataccgattt gggaggttct tggaatcttt gggaaaactt
    10921 agctgctcaa tctgatgaat ttggacatcc taaagtcttc tacaaagata ttccaaaaga
    10981 gtcatggttt gtttctgcta ctgcaacaat aaaagatcca gctattgaac cttatataga
    11041 gcgtttaaca catcgtgacc tccatgatgg taaagtaaat accggaggca ttgttacagt
    11101 tacagattct aattggatga tgagttttgc tatccatcgc caaccgcatt tcaaagagca
    11161 aaaggaaaat gaaacaattg tttggattta tggactctat tcaaatgtgg aaggtaatta
    11221 tattaaaaaa ccaattgaag aatgtactgg tcgagaaatc acagaagagt ggttatatca
    11281 cttaggtgtt cctgaaatga agattcatga tttatctgat aaacaatatg taagtacagt
    11341 tcctgtttat atgccttaca ttactagtta tttcatgcct cgagttaaag gtgaccgacc
    11401 agatgttatc ccacaaggat ctgttaacct agccttcatt ggtaattttg cagaatctcc
    11461 atccagagat acagtattta caacggaata ttctattaga actgctatgg aagctgttta
    11521 tactttctta aatattgaac gtggtgttcc agaagtcttt aattcagcat ttgatattcg
    11581 tgtcttgttg caatctttat actatctcaa tgataagaaa tctgttgaag atatggattt
    11641 accaatacct gccttaatgc gtaaagtagg catgaaaaaa attagaggaa cttatttaga
    11701 agaattactt cgagaagctc atcttttata atgtaaaata aaatcgagaa aacttgtttt
    11761 tctcgatttt ttagttttac aacgactaaa atcaccaaag aaaatgaaat tttgttataa
    11821 tgtagtcact tactgtacga aaaggaagaa taatttgcaa gaatactcta tcgaaattac
    11881 tttacaacat ccagatgata tgatgtcact ctttggcagc aatgagcgcc atttaaaatt
    11941 aatcgaagaa aatttagatg ttatcattca tgctagaact gaacgtgttc aggtattagg
    12001 tgactctgaa gaagcagtag aaaccgctcg gttaaccatt gaagcattat tagtgcttgt
    12061 taaccgcggg atgactgtga atacttcaga tgttgtaaca gccttatcca tggcacaaaa
    12121 tggttcaatt gataaattcg ttgcccttta tgaggaagaa attattaagg atagctatgg
    12181 taagcctatc cgtgttaaga ctttagggca aaatatatac gttgatagcg ttaaaaatca
    12241 tgatgttgtt tttggtattg ggcctgctgg aacgggaaaa acttttttag cagtcactct
    12301 agcagtaacg gcactaaagc gtggtcaagt taaacgaata atcttaacga gaccagcggt
    12361 tgaagctggt gaaagccttg gtttcttgcc tggagatctc aaagagaaag ttgatcctta
    12421 tttgagacca gtttatgatg ccctatatca aattttaggt aaagagcaaa caagtcgctt
    12481 aatggagcgt gaaatcatcg aaattgctcc attggcttac atgcgtggtc gtactttaga
    12541 tgatgcattt gtcatattag atgaagcgca aaacaccact ataatgcaaa tgaaaatgtt
    12601 cctaacacgt cttggcttca attctaaaat gattgtaaat ggcgatgtta gccaaattga
    12661 cttaccaaag aatgttaaat ctggtttgat tgatgctgtt gagaaattac gaaatatcaa
    12721 aaagattgac tttattcatt tatcagcaaa ggatgttgta agacaccctg tcgttgctga
    12781 aataataaat gcttattcag attcagaaag tagtcacaag ctacaatctg ataaggactg
    12841 atgtttgcca ctaggagacg ttgttagaga gtgtgtcaat atgtaaaaaa cgcctaatgc
    12901 tttaggcgtt tttatgatgg tgtacttcaa cttaacctat aaagtgttga aaattttctt
    12961 taagcatgac gtcgagatgc attattatat cgtttaatgt ttctttaaca gtgtgttcgt
    13021 tggagttgtg atgacatagt ttaatgatat tttcttcgaa atgtttagga agcaatgtag
    13081 cattcttctt agcataggaa atcattcttt tttcgccagg atgtgtcaac ttattaagtg
    13141 caaaaataat atcaaagtag gaatctaaga aagcagtagt tctatggtga gtactaacaa
    13201 agtcttggcg tttaagggct tttataattt gtttatcgta agagggaagc ttgccagtta
    13261 ataagttacg attttgaatg ataatttgtt tttgtaactg actgggataa gaaacttcga
    13321 atctctcttt gagtgattgt aattgatttt caggatcata gagtatttgg caattgatga
    13381 ggttatgcca aaaacaagta gtgtatccaa ttcgagaatt gtggtgttca acgacatctt
    13441 ctaagtctga taaaaagtta tctatgttac gatagagaat atcaatatca gttccgtcgt
    13501 ttaaagtacc attatcttca agctcccagt aatgattgtt aagttcaatg taatgacagt
    13561 gcttgttaag gatacgttta cgactagtga tatcaggagt tgctgcacaa taaacataca
    13621 aatcataatc ggaagattgg tcaaaactgt caccagagcg tgagcctccg agtgctatag
    13681 caacaacatt tggaagttta gagaaatctt gccagagttt tgtgaaatgg tccataaatg
    13741 ctctcctaaa aatgtaaaat attgatgtgt ctccattcta cagggctatc tctttttagt
    13801 caataattaa tagtaagtta ataaaagagg taacggttct aatattcaag aagtgtatat
    13861 gagtgatttg aaaaggagat attttatgct acaatacgta catattagtt taaacaaggt
    13921 gatagaatga aacaagatta tatttcctac attaggtcaa aagttggaca tgagactatc
    13981 tttttaacat attctggtgg tattttgaca gatggtaagg gacgtgtctt actccaatta
    14041 agagctgata agaattcttg gggaattatt ggtggacgta tggagttagg tgagtcatca
    14101 gtagatacat tgaaaagaga atttttcgaa gaaactggat tgagagtgga accaatcaga
    14161 ctgttaaatg tttatactaa ctttcaagac tcttatccca atggcgataa agcacaaacc
    14221 gtcggtttta tttatgaagt aagctgtcca aaaccagtta atatagaagg ttttcataat
    14281 gaggaaacat tgcaattgga ctatttttct aaagaagacg tcaaaaatat tacgatcgta
    14341 aatgaacaac accaattaat tttagatgaa tatttttcac aaacattcca aatggggcgt
    14401 taaaaggatg ggaaatacta ggtgtgactt taaagaactt ctaactttta ttgatatgtt
    14461 gttaggcatt cttgtagaaa caaatgttaa gaaaataagg agttctcatt tgtatatttt
    14521 ataattatta aaaagaaaaa cagtagcttg caataattta gtgactgttt ttttagaaag
    14581 cataagaagt acagaaaaag aaaattatgg aatattggat ggtggggata tgctttactt
    14641 acggtattgg aatgatatac ttaaagaaat ccagttttag aaatccaaat ttctaagtaa
    14701 aatagcataa aggagagtag atgaatgaag ataacattac acggcgttgc tgaaacgtta
    14761 ttaatcacct tatatattag ggctaaagat gcgatggcta aacatccgat acttaatgat
    14821 caaaaatcct tagcaattgt tgaacagata gaatatgatt ttgataaatt cgataattca
    14881 gaagcttctt tttatgcaac attagctaga attcgcgtta tggatagaga aatcaaaaaa
    14941 tttattagag aaaatccaaa tagtcaaatc ctttcaattg gttgtggact tgatacaagg
    15001 tttgaaagag tcgataatgg acaaattagg tggtataacc ttgatttgcc agaggttatg
    15061 gagataagaa aattattttt tgaagagcat gaaagagtta ctaatatagc aaaatcagcc
    15121 ctagatgaaa cttggacacg ggaggtaaat ccccaaaatg ccccttttct aatcgtgtca
    15181 gaaggtgttt taatgtttct aaaagaagat gacgtagaga cttttcttca tatcctgaca
    15241 aattcattta gccaatttat ggcacaattt gatttgtgtc ataaggaaat gattaataaa
    15301 ggaaagcaac atgatacagt aaagtatatg gatacagaat ttcagtttgg tatcacagat
    15361 ggtcatgaga ttgtggattt agaccctaaa ttaaagcaaa taaatctgat taactttaca
    15421 gatgagatga gcaaatttga gttaggcaca cttcgctctt tacttccaac aattcgtaaa
    15481 tttaataatt gtttaggtgt gtacgaatat aaagcatctg agaaaaagta acaaaaatct
    15541 atttatataa agagacattc taaatg
//
LOCUS       DATROQ010000040        15475 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.040, whole genome shotgun sequence.
ACCESSION   DATROQ010000040 DATROQ010000000
VERSION     DATROQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15475
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.040"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(244..726)
                     /locus_tag="VBC94_001160"
     CDS             complement(244..726)
                     /locus_tag="VBC94_001160"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEO6979435.1"
                     /translation="MTKEVVVESFELDHTIVKAPYVRLISEEVGPVGDIITNFDIRLI
                     QPNENAIDTAGLHTIEHLLAKLIRQQINGLIDCSPFGCRTGFHMIMWGKQDATEIAKV
                     IKSSLEAIAGGVTWEDVPGTTIESCGNYKDHSLHSAQEWAKLILSQGISDNAFERHIV
                     "
     gene            915..2522
                     /locus_tag="VBC94_001161"
     CDS             915..2522
                     /locus_tag="VBC94_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEO6979436.1"
                     /translation="MFNIILAMVCALIGLIIGYVAISMKMKSSKEAAELTLLNAEQDA
                     VDLRGKAEIEAEHIRKAAERESKAHQKELLLEAKEEARKYREEIEKEFKSDRQELKQM
                     EARLTDRASSLDRKDENLSNKEKMLDSKEQSLTDKSRHINEREQEIATLETKKVEELS
                     RIAELSQEEAKDIILADTEKDLAHDIATRIKEAEREVKDRSNKIAKDLLAQAMQRLAG
                     EYVTEQTITTVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVVLSGFDPI
                     RREIARMTLESLIQDGRIHPARIEELVEKNRLEMDQRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSYGQNVLRHSVEVGKLAGILAGELGENVDLARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPIVVNTIASHHGDVEPDSVIAVIVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIANGFEGVQNAFALQAGREIRIMVQPGKVSDDQVVIMSHKVRE
                     KIEQNLDYPGNIKVTVIREMRAVDFAK"
     gene            2651..3547
                     /locus_tag="VBC94_001162"
     CDS             2651..3547
                     /locus_tag="VBC94_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979437.1"
                     /translation="MAKDIIKVDHIFKSIGQKTILEDISFSIASNQCVALIGPNGAGK
                     TTLMSTLLGDISISSGALTIFNLPAHHNRLKYKVAILPQENVLPSKFTVRELIDFQRC
                     LFPEVLPMSLILDYLQWSDAHLQQFTETLSGGQKRLLAFVLTLVGKPQLLFLDEPTSG
                     MDTSTRQRFWELVATLKKEGDTIVYSSHYLEEVEHTADRILVLHKGKLLRDTTPFAMK
                     QEKTEKLFTVPLSYQKLLPTYLITECEAKSDSITFVTGEAETVWKILADNGCPIEAIE
                     MTNRTLLNRIFETTKEVKHENL"
     gene            3534..4274
                     /locus_tag="VBC94_001163"
     CDS             3534..4274
                     /locus_tag="VBC94_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6979438.1"
                     /translation="MRIFKALLKMEFLLTKRQLANLIMAIGMPVAFFLFFSGFMGEGL
                     TKAIEAIYVRNYMITMAGFSSLSFAFFTFPFSMKDDQLSNRMQLLRHSPVPMWQYYLA
                     KIIRILFYYCLAITVVFLTGHILRQVSMPIEQWMQSFLLLLGGATCFIPFGLLVSYFK
                     NTELMSMVANICYMSLAVLGGMWMPITMFPKWLQGLSKLTPTYHLTQVILSPFANSFA
                     VFSLIILIGYGIIMLVIAYLLSQKRHSI"
     gene            4271..5356
                     /locus_tag="VBC94_001164"
     CDS             4271..5356
                     /locus_tag="VBC94_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6979439.1"
                     /translation="MKNKKNNYDLMYFIPLVFLIYPIGGILYYHYPFWTLFFTLAFVG
                     AYLYSIIIRGESKYHMIAWFTMLTYIFYMTVFINSGFIWYIYFLSNLLVYRFRDKLKS
                     FRFISFACTLATMVFLCFFKASDFGDRIMFLIVPIFCIGYMWIAIENRNSEEQREKIA
                     EQNQYINILSAENERNRIGRDLHDSLGHTFAMMTLKTELALKLLEKRNYDKVQKELSE
                     LNHISHQSMSEVRQIVSNLKYRTVVEEIDELYRLFQLSNIKLTVVNKLETSQLSPVTQ
                     STITMILKELSNNIVKHAEADSVELSLVRQGATINIEMIDNGCGFTNLDGDELHSIQE
                     RLTIVEGTLTILSRSKPTHIQVILKEV"
     gene            5358..5948
                     /locus_tag="VBC94_001165"
     CDS             5358..5948
                     /locus_tag="VBC94_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6979440.1"
                     /translation="MKLLVAEDQSMLRDAMCQLLLMEESVSTIDQAGNGEEAIAILSN
                     KAIDVAILDVEMPILSGLDVLEWVRKYQNVKVIIVTTFKRSGYFQRAIRSNVDAYVLK
                     DRSVADLMKTIQKVLSGGKEYSPELMENVISNPLSKQEIKILSLIAQGKTSKEIACTL
                     FLSNGTVRNYTSNIFDKLGVNNRLEALKIAREKDWL"
     gene            5998..6702
                     /locus_tag="VBC94_001166"
     CDS             5998..6702
                     /locus_tag="VBC94_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEO6979441.1"
                     /translation="MQKFKERFDTLSDAILAIAMTILVLEIKTPTTMGDIGDFTRNIG
                     LFIVSFVVVFNFWYERAQNSLDAQKTNDEIIALDIIEHLGICLIPLFTKFMISFENHN
                     FAVMAYGLLTLLVGLTSDIIRIRLASYDLVTIPSELKERVIKVMTTFAIRSVVVRFII
                     IILAYFLPEVGIFAYLVIPLFMFWRRSRAGKYYTKKGIEAPSYIKLFIQIGERVPEDF
                     TNSSSGISGVKRLYKK"
     gene            6869..7498
                     /gene="gmk"
                     /locus_tag="VBC94_001167"
     CDS             6869..7498
                     /gene="gmk"
                     /locus_tag="VBC94_001167"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEO6979442.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFDYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEALIKEGQMLEYAEYVGNYYGTPLSYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFIFLTPPDLEELEERLVGRGTDSPEVIAQRIERAKEEIALM
                     REYDYAVVNDQVSLAAERVKRVIEAEHYRVDRVIGRYTNMVKETDKKLS"
     gene            7521..7835
                     /locus_tag="VBC94_001168"
     CDS             7521..7835
                     /locus_tag="VBC94_001168"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEO6979443.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGEKATQDFKSV
                     KSTLRALEEIESGNVVIHPDPSAKRASVRARIEAERLAKEEEERKIKEQIAKEKEDGE
                     KI"
     gene            7909..10299
                     /locus_tag="VBC94_001169"
     CDS             7909..10299
                     /locus_tag="VBC94_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEO6979444.1"
                     /translation="MARKLAQVIVDIPLMQTDKPFSYAIPKDLEDLVQVGARVHVPFG
                     RGNRLLQGFVVGFRDDDELETKDIAGVLDFEPVLNQEQLDLADQMRHTVFSYKISILK
                     SMLPSLLNSQYDKLLLATDTLPSEDREDLFGHKTEIVFSSLSSQDAKKAGRLIQKGFI
                     EVQYLAKDKKTIKTEKIYKINRTLLEESQIAARAKKRLELKEFLLENPQPGRLTALNK
                     QFSSPVVNFFREEGIIEVIEKEASRSDNYFKGILKTDFLDLNQEQAKVVKIVVDRIGK
                     DQNKPFLLEGITGSGKTEVYLHVIDNVLKLGKTAIVLVPEISLTPQMTNRFISRFGKR
                     VAIMHSGLSDGEKFDEWRKIKSGQAKVVVGARSAIFAPLENIGAIIIDEEHESTYKQE
                     SNPRYHARDVALLRAEYHKAVLLMGSATPSIESRARASRDVYKFLELKHRANPKARIP
                     QVEIIDFRNFIGQQEVSNFTPYLLDKIRDRLDKKEQVVLMLNRRGYSSFIMCRNCGYV
                     DQCPNCDISLTLHMATKTMNCHYCGFEKPIPRTCPNCNSKSISYYGTGTQKAYEELLK
                     VIPDAKILRMDVDTTRQKGGHESILKRFGNHEADILLGTQMIAKGLDFPNVTLVGVLN
                     ADTSLNLPDFRSSERTFQLLTQVAGRAGRAEKEGEVVIQTYNPNHYAIQLAQKQDFEA
                     FYQYEMNIRRQLGYPPYYFTVGLTLSHKDEEWLIRKSYEVLSLLKQGFSDKVKLLGPT
                     PKPIARTHNLYHYQIIIKYRFEDNLELVLNRLLDITQDKENRDLRLAIDHEPQNMM"
     gene            10346..11281
                     /locus_tag="VBC94_001170"
     CDS             10346..11281
                     /locus_tag="VBC94_001170"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEO6979445.1"
                     /translation="MTKLLFMGTPDFSATVLKGILADGKYDVLAVVTQPDRAVGRKKE
                     IKMTPVKEVALENNIPVYQPEKLSGSPELEQLMTLGADGIVTAAFGQFLPTKLLESVG
                     FAINVHASLLPKYRGGAPIHYAIINGEKEAGVTIMEMVAKMDAGDMVSKASVEITDED
                     NVGTMFDRLAVVGRDLLLDTLPGYLSGDIKPIPQNEEEVSFSPNISPDEERIDWNKSS
                     RDIFNHVRGMYPWPVAHTLLEGNRFKLYEVTMSEGKGSPGQVIAKTKNSLTVATGDGA
                     IELKSVQPAGKPRMDIKDFLNGVGRNLEIGDKFGE"
     gene            11271..12593
                     /gene="rsmB"
                     /locus_tag="VBC94_001171"
     CDS             11271..12593
                     /gene="rsmB"
                     /locus_tag="VBC94_001171"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEO6979446.1"
                     /translation="MANDWKKSARGLALMTLEEVFDKGAYSNIALNKSLKKSRLSDKD
                     RALVTEIVYGTVARKITLEWYLSHFIVDRDKLELWVYHLLLLSLYQLLYLDNIPDHAI
                     VNDAVTIAKNRGNKKGAEKLINAVLRRVSSETLPEIASIKRQNKRYSVAYSMPVWLVK
                     KLIDQYGETRALAIMESLFERNKASLRVTDLSQKQTIKETLNVRDSHIAETALVADSG
                     NFASTSFFQDGLITIQDESSQLVAPTLKVSGNDQVLDACSAPGGKTSHIASYLTTGAV
                     TALDLYDHKLELVMENAKRLGLSDKIKTKKLDASKAHEYFPEDTFDKILVDAPCSGIG
                     LIRRKPDIKYNKANQDFEALQEIQLSILSSVCQTLRKGGIITYSTCTIFEEENFQVIE
                     KFLENHPNFEQVELSHTQEDIVKRGCISISPEQYHTDGFFIGQVKRIL"
     gene            12631..13368
                     /locus_tag="VBC94_001172"
     CDS             12631..13368
                     /locus_tag="VBC94_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEO6979447.1"
                     /translation="MEISLLTDIGQRRSNNQDFINQFENKAGVPLIILADGMGGHRAG
                     NIASEMTVTDLGSDWAETDFSELSEIRDWMLVSIETENRKIYELGQSDDYKGMGTTIE
                     AVAIVGDNIIFAHVGDSRIGIVRQGEYHLLTSDHSLVNELVKAGQLTEEEAASHPQKN
                     IITQSIGQANPVEPDLGVHLLEEGDYLVVNSDGLTNMLSNADIATVLTQEKTLDDKNQ
                     DLITLANHRGGLDNITVALVYVESEAV"
     gene            13368..15323
                     /gene="pknB"
                     /locus_tag="VBC94_001173"
     CDS             13368..15323
                     /gene="pknB"
                     /locus_tag="VBC94_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEO6979448.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLARDLILDNEEVAIKVL
                     RTNYQTDQIAVARFQREARAMAELTHPNIVAIRDIGEEDGQQFLVMEYVDGFDLKKYI
                     QDNAPLSNNEVVRIMNEVLSAMSLAHQKGIVHRDLKPQNILLTKKGTVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSILAENKSVPQALENIVIKATAKKLTDRYKTTYEMGRDLSTALSST
                     RHREPKLVFNDTESTKTLPKVTSTVSSLTTEQLLRNQKQAKTTEKITPDSASNDKTKS
                     KKKASHRLLGTIMKLFFALCVVGIIVFAYKILVSPTTIRVPDVSNKTVAQAKMTLENS
                     GLKVGAIRNIESDSVSEGLVVKTDPAAGRSRREGAKVNLYIATPNKSFTLGNYKEHNY
                     KDILKDLQGKGVKKSLIKVKRKINNDYTTGTILAQSLPEGTSFNPDGNKKLTLTVAVN
                     DPMIMPDVTGMTVGEVIETLTDLGLDADNLVFYQMQNGVYQAVVTPPSSSKIASQDPY
                     YGGEVGLRRGDKVKLYLLGSKTTNNSSSTPIDSSASSSTGTTTSDSVSSSTDASTSDS
                     SSTSTSSSTLPSDSTTNTGTANNPLTQ"
BASE COUNT         5141 a         2466 c         3125 g         4743 t
ORIGIN      
        1 tgatagttta gattattaat ataaagagca aaaccagttt tttgtctaca ccctaaaagt
       61 tagaaaatca gtctaacgat tagggtgttt tttgtattcg catttagatg tgtacgactg
      121 ctatgaggat attagggaaa aattaaaagg attttgctct ctatgataca gaacaaaatc
      181 ctttgtttaa attaataaga caacttttgg aattccaaaa gttgactttt attttttatt
      241 agatcagaca atgtggcgct caaaagcatt gtcagaaatc ccttgggaaa gaattaattt
      301 cgcccattct tgagcagagt ggagactgtg gtctttataa tttccacaag attcaatggt
      361 tgttccagga acgtcttccc aagtgacccc accagcaata gcctctagac tagatttgat
      421 gactttagca atttcagtag catcctgttt tccccacata atcatgtgaa aaccagttct
      481 acaaccaaaa ggagaacaat caatcaaacc attaatttgt tggcggatta acttcgccag
      541 aaggtgctcg atagtatgta aaccagcagt atcaatagca ttttcattag gctgaataag
      601 gcggatatca aagttagtaa tgatgtctcc gaccggtcca acttcttctg aaattaaacg
      661 aacgtagggt gctttaacga tggtgtgatc taattcaaaa ctctctacga caacttcttt
      721 tgtcataagt tttctccttt tgtattacat tctagagact aaaggagatg ttgaaaattt
      781 ctcctaatcc ctttaaataa cattctaatc atatcataaa tgtctataat tttaaattgt
      841 gaaaacgcct tttttatgat aaacttaaaa gtggactttc tatatgaaat ctagaaaaaa
      901 attgaggtat aaacatgttt aatattatat tagcaatggt ttgtgcactc attggtttga
      961 ttattggtta tgtggctatt tcaatgaaaa tgaaatcatc aaaagaagct gcagaactaa
     1021 ctcttttaaa tgcagaacaa gatgccgttg atttacgtgg taaagcagaa attgaagcag
     1081 aacacatccg taaagcagcg gaacgtgaaa gtaaagctca ccaaaaagaa ttacttttag
     1141 aagcaaaaga agaggctaga aaatatcgtg aagagatcga aaaggaattt aaatctgata
     1201 gacaagagtt gaaacagatg gaggcacgtt taacagatcg tgcatcatct cttgatcgta
     1261 aagatgaaaa tctatctaac aaagaaaaaa tgttagatag caaagaacaa agtctaaccg
     1321 ataaatctag acatattaat gagcgcgaac aggaaattgc tactttagaa acaaaaaaag
     1381 tagaagaatt gtcacgcatc gcagagctta gtcaagaaga agctaaagat atcattttag
     1441 ctgataccga aaaagattta gctcatgata ttgcgacacg tattaaagaa gcagaacggg
     1501 aagtaaaaga tcgatcaaac aagatagcaa aagacttgct agcccaagct atgcaacgtt
     1561 tagctggtga atatgttact gaacaaacaa ttacaactgt tcatctccct gatgataata
     1621 tgaagggacg tatcattggt cgtgaaggac gtaatattcg tactctggaa agtttgacag
     1681 gtattgatgt tatcatagat gatacgccag aagtcgttgt cttatcaggt ttcgacccca
     1741 ttcgtcgtga aattgctcgt atgactttag aatctttgat tcaagatgga cgtatccatc
     1801 cagctcgtat tgaagagttg gttgaaaaaa atcgtttgga aatggatcaa cgtatccgtg
     1861 agtatggtga ggcagcagct tatgagattg gtgcaccaaa tctgcatcct gatttgataa
     1921 aaattatggg acgtttgcag ttccgtacat catatggtca aaatgtactt cgtcattcag
     1981 ttgaagttgg taaacttgca ggtattttag ctggagaact aggagaaaat gttgatttag
     2041 ctcgtcgggc tggcttcctc catgatatgg ggaaagctat tgatcgcgaa gttgaaggca
     2101 gtcacgttga aattggtatg gaatttgctc gtaagtacaa agagcatccg attgttgtta
     2161 atactattgc aagccaccat ggtgatgtag aacctgacag cgttattgca gtaattgttg
     2221 ctgctgcaga cgcccttagt tcagcacgac caggtgcgcg taatgagtca atggaaaatt
     2281 atatcaaacg tctgcgtgat ttagaagaaa ttgctaatgg ctttgaggga gtgcaaaatg
     2341 cctttgcact tcaagccgga cgtgaaattc gtatcatggt acaacctggt aaagtttctg
     2401 atgatcaagt agttattatg tcgcataagg tacgtgaaaa aatcgaacaa aacttggatt
     2461 atccaggtaa tattaaggta acagttattc gtgaaatgcg agctgttgat tttgcaaaat
     2521 agtcaaaagg agagcttgtt agctctcctt ttgttataga gttgacattt gtcatatttt
     2581 gaaatgacaa aaatatctaa aaagtttcct tctgattttt tatactatga ttatagtaaa
     2641 ggagaaccaa atggcaaaag atataattaa ggttgatcat atatttaaaa gcatcgggca
     2701 aaaaacgatt ttagaagata tatcatttag tatagcttct aaccaatgtg tagcattgat
     2761 tggacctaat ggtgcaggga aaacaactct gatgtcaact ttattaggtg atattagtat
     2821 ttcttcagga gcattgacta tttttaatct gcctgcacac cacaataggt taaaatataa
     2881 ggtggccatc ttaccacaag aaaatgtatt gccctcaaag tttacagtga gagaactcat
     2941 tgatttccaa aggtgtttat ttccagaagt gctgccaatg tctctgattt tagactatct
     3001 acagtggagt gatgcgcact tgcaacaatt tacagagact ttatctggag ggcaaaaacg
     3061 acttttagct tttgttttaa ctttggttgg taagccccaa ttactatttt tagatgaacc
     3121 tacttccgga atggatactt ccacacgtca acgattttgg gagctggttg cgacactaaa
     3181 aaaagaaggt gacacaattg tctattctag tcattatctc gaagaggtag aacatacagc
     3241 tgataggatt ttagtacttc ataaaggaaa gttattacgc gatacaaccc cctttgccat
     3301 gaagcaagaa aaaaccgaaa agttattcac cgttccgctt agttatcaaa aattattacc
     3361 tacctatttg attacagagt gtgaagccaa gagtgatagt ataacgtttg ttactgggga
     3421 ggctgaaact gtatggaaaa tactggcaga taatggttgt cctattgaag ctattgagat
     3481 gaccaataga actttgttaa atcgtatttt tgagactact aaggaggtaa aacatgagaa
     3541 tctttaaagc attattgaaa atggaatttc ttttgacaaa acgtcaactt gccaatctca
     3601 ttatggctat aggtatgcca gtggcttttt ttctattttt ttcaggattt atgggcgaag
     3661 gactcactaa agccatcgaa gctatctacg ttcgtaatta tatgataacg atggcaggat
     3721 tctcaagtct tagctttgct ttttttactt ttccattttc tatgaaagat gatcaactct
     3781 caaaccgaat gcaattatta agacactcac cagtaccaat gtggcaatat tatctagcaa
     3841 aaattattcg tattttattt tactattgcc tagcaataac tgttgttttc ctaacagggc
     3901 atattttaag gcaagttagt atgccaatag aacaatggat gcagagcttt ttattgctat
     3961 taggaggagc aacatgtttt attccttttg gattgctcgt ctcctacttt aaaaatactg
     4021 aactaatgtc aatggtagcc aatatttgtt atatgtcatt agctgttctt ggtggcatgt
     4081 ggatgccaat tacaatgttc ccaaaatggt tgcaaggttt atcaaagtta acccctactt
     4141 accatttgac tcaagttatc cttagtccgt ttgctaatag ttttgctgtt ttttctctta
     4201 ttattctgat aggctatggt attataatgt tagttatcgc ttatctacta tctcagaaga
     4261 ggcattcaat ttgaaaaata aaaaaaataa ctatgattta atgtatttta ttccgttagt
     4321 atttttgatt taccccatag gagggattct ctattatcac tacccttttt ggactttatt
     4381 ttttacgcta gcttttgtag gagcatattt atactctatt attatcaggg gcgaatcaaa
     4441 gtatcacatg attgcctggt ttacaatgtt gacatatatt ttctacatga cagtttttat
     4501 aaatagtggt ttcatctggt atatttactt tctgagtaat ttactcgtct atcgctttag
     4561 agataaattg aagtctttta gattcattag ttttgcttgc actctagcca caatggtatt
     4621 tttatgcttc tttaaggcat cagactttgg tgatagaatc atgtttctaa ttgttcctat
     4681 tttttgtatc ggttacatgt ggattgctat tgaaaataga aatagcgaag aacaaagaga
     4741 aaaaattgca gagcaaaatc agtatatcaa tattttgtca gctgaaaatg aacgtaaccg
     4801 tatagggcgt gatttgcatg atagtttagg acatacattt gctatgatga cactaaagac
     4861 agaactagcg ttaaagttgt tagaaaaaag gaactatgat aaggtacaaa aagaactatc
     4921 agaattaaat catattagtc atcaatcgat gagtgaggtc cgtcaaattg tttctaacct
     4981 caagtatcgt acagtagtgg aagaaattga tgagttgtac cgcttgtttc aattatctaa
     5041 catcaagtta acagttgtta ataagttaga gacgtcacaa ctaagtccag taacacaatc
     5101 tacaatcaca atgatattaa aggaattatc caataatatt gttaagcatg ctgaagctga
     5161 tagcgtggag ttgagtttag taaggcaagg tgctactata aatattgaga tgattgataa
     5221 tggttgtggt tttacaaacc ttgacggaga tgagttgcat agtattcaag agagattaac
     5281 tatcgttgaa gggacactaa cgattttgtc ccgaagtaaa ccaactcata ttcaggtgat
     5341 attgaaagag gtataagatg aaattacttg ttgctgagga tcagtccatg ctacgagacg
     5401 ctatgtgcca attacttttg atggaagaaa gtgtttctac tattgatcaa gctggaaatg
     5461 gtgaagaagc tattgccatt ttatccaata aagccataga tgttgcaata ttggatgttg
     5521 agatgccgat attatcaggt ttagatgtct tagagtgggt tagaaaatac cagaatgtta
     5581 aggtcataat tgtaacaacc tttaagcggt caggttattt ccaaagggct attcgttcaa
     5641 atgtagatgc ttatgttttg aaagaccgct cagtagctga tttgatgaaa acgattcaaa
     5701 aagttttatc tgggggtaaa gaatattcgc ctgaattaat ggaaaacgtt atatctaatc
     5761 ctttgagtaa gcaagaaata aaaattcttt ccctaattgc tcaagggaag accagcaaag
     5821 aaattgcatg cactttattt ttatcgaatg gtacagttcg taattacacg tcaaatattt
     5881 ttgataaatt aggagttaat aatcgtttgg aggcacttaa aatagcacga gaaaaggatt
     5941 ggctgtgata gtttttttcc agtcgagatg ccataatgta ttaagaggga tatatgtatg
     6001 caaaaattta aagaacgttt tgatacgctt agtgatgcaa ttttggccat tgctatgacg
     6061 attttagttt tggagataaa aactccgact acgatgggtg atattggtga ctttacacgg
     6121 aacataggtt tatttattgt tagctttgtt gttgttttta atttttggta cgaaagggca
     6181 caaaatagcc tagatgctca aaagactaat gatgaaatta tagctcttga tatcattgaa
     6241 cacctaggta tttgtttgat tcctctcttt actaaattta tgatttcttt tgagaatcat
     6301 aattttgctg ttatggctta tggactctta acgttattag ttggtctcac atcagacatt
     6361 attcggattc gtttggcaag ctatgattta gtgaccattc ctagtgaatt gaaagagcgg
     6421 gttataaaag tcatgacaac ttttgccatt cgttcagttg tggttcgctt tatcattatt
     6481 atcctcgctt attttctacc tgaggtaggg atatttgcat atttagtcat accgctcttt
     6541 atgttttggc gtcgctctag agcaggaaaa tactatacta aaaagggcat tgaggcaccc
     6601 tcttatatca agctttttat ccaaattgga gagagagttc ctgaggattt tactaattca
     6661 agtagtggaa taagtggggt aaaaagactc tataaaaaat aataggagac cttgaaaaaa
     6721 aggtttcttt ttgttaaact aatgagaaga aactttattt agggagcaat ttatgaattg
     6781 ctataaaagt taatagttaa ggtaagaacc agattgttct ggtcttttta acacttaaca
     6841 cccgatctat taatttgagg agtgtgtaat gtctgaacgt ggtttgctaa ttgttttctc
     6901 aggaccatct ggtgttggta aggggacagt taggcaagaa attttttcaa cccctgacca
     6961 taaatttgat tattccgttt caatgactac tcgtccacaa cgcccaggcg aagttgatgg
     7021 agttgattat ttcttccgta cacgtgaaga gtttgaagcg ttgattaaag aaggtcaaat
     7081 gcttgagtat gcggagtatg ttggtaacta ctatggaact cctttaagtt atgtcaacga
     7141 gactttagac aaagggattg atgtttttct tgaaatagaa gttcagggag cgcttcaagt
     7201 taaatcgaag gttccggacg gtgttttcat tttcttaaca ccaccagatt tagaggaatt
     7261 agaagagcgt ttagtaggac gtgggacaga tagtccagaa gtgattgccc aacgtattga
     7321 acgtgccaaa gaagaaattg ctttgatgag ggagtatgac tatgctgttg tcaatgatca
     7381 agtgtcatta gcagcagaac gtgttaaacg tgtcattgaa gcagaacatt atcgtgtgga
     7441 tcgtgttatt gggcgttata ccaacatggt caaagaaact gataaaaaat taagttaaaa
     7501 atagaaaaga gaattgcctt atgatgttaa aaccttctat cgacactttg ttggataaag
     7561 ttccttcaaa atattcttta gttattttac aagctaagcg tgctcatgaa ttggaagctg
     7621 gtgagaaagc cactcaagat tttaaatctg tgaaatcaac acttcgcgct ttagaagaaa
     7681 tcgagtcagg taatgttgta attcacccag atccatcagc aaaacgtgcc tcagttcgcg
     7741 ctcgtatcga agctgaacgc cttgctaaag aagaagaaga acgtaaaatc aaagagcaaa
     7801 ttgctaaaga aaaagaagac ggcgaaaaaa tttaagcaag tcaaaagtga gaggttgaaa
     7861 cattctgtat cagcctcttt tcatatttta gagaattagg gagggtttat ggcaagaaaa
     7921 ttagcacagg ttattgttga tattcctctg atgcagacag ataaaccatt ttcgtatgcc
     7981 attcctaaag atttggaaga tttggtacaa gtaggagcac gcgttcatgt tccttttgga
     8041 agagggaatc gtcttttgca aggctttgtt gtcggtttca gagatgatga tgaattagag
     8101 actaaggata ttgcgggggt cctagatttt gaacctgttt taaatcagga acaactagac
     8161 ttagctgatc agatgcgcca tactgtcttt tcttataaaa tatctatttt gaaatcaatg
     8221 ttaccaagtc ttttgaattc gcaatatgat aaactcttgt tagcaacaga cacattacca
     8281 tcagaagata gagaagatct atttggtcat aaaacggaaa ttgtcttttc aagtttgtct
     8341 tctcaagatg ccaaaaaagc aggcagatta attcaaaaag gtttcataga agttcaatat
     8401 ttagccaaag ataagaaaac aataaaaaca gaaaagattt acaaaatcaa cagaactctg
     8461 ttagaagaga gtcaaatagc agcaagagcg aaaaagagac tggagttgaa agaattcctg
     8521 ttagaaaatc ctcaaccagg gcgcctcaca gctttaaaca aacaattttc atctcctgta
     8581 gttaattttt tcagagaaga aggaatcatt gaggtcattg aaaaagaagc tagtagatct
     8641 gataattatt ttaaaggaat cctaaaaacg gattttttgg atttaaatca ggaacaagct
     8701 aaagtagtta aaatagttgt tgaccgaatt ggtaaggatc agaataaacc cttcttgttg
     8761 gaggggatta cagggtctgg taagacagag gtatatcttc atgtcattga taacgtttta
     8821 aagttgggaa aaacagcgat tgttctcgtt ccagaaattt ctttaactcc ccagatgacg
     8881 aatcgtttta tttcccgttt tggaaagcga gttgctatta tgcactcggg cttgtcagat
     8941 ggtgaaaaat ttgatgagtg gcgaaagata aaatcaggtc aagctaaagt tgtcgttgga
     9001 gctcgttcag caatttttgc tcctttggaa aatatcggag ccatcattat agatgaagaa
     9061 catgaatcaa cttataagca agaatctaac ccaaggtatc atgcgcgcga tgtggccttg
     9121 ctacgtgcag aatatcataa agctgtgtta ctaatgggat cagcaactcc aagtatcgaa
     9181 agccgagcta gagctagtcg ggatgtttat aaatttttag aattaaaaca tcgtgctaat
     9241 cctaaagcac gtattcctca ggttgaaatt attgatttta gaaattttat tggtcaacaa
     9301 gaagttagta atttcacacc atatttacta gataaaatcc gagatagatt agataagaaa
     9361 gagcaagtgg ttcttatgct caatcgtaga ggttattcta gttttattat gtgtcgaaac
     9421 tgcggctacg ttgatcaatg tcctaattgt gacatttctc tgactttaca tatggctaca
     9481 aagaccatga attgtcatta ttgtgggttt gaaaagccta ttccaagaac atgtccaaat
     9541 tgcaatagta aaagtatctc ttactatggt actggaaccc aaaaagctta tgaagaactt
     9601 ttgaaagtta tcccagacgc aaagatttta cggatggatg ttgatacgac aaggcaaaaa
     9661 ggcggtcatg agtctatttt gaagcgtttc ggaaatcatg aagctgatat tttattggga
     9721 acacagatga ttgctaaggg attggatttt ccaaatgtta ctttagttgg agtactaaat
     9781 gcagatactt ctctgaattt acctgatttt cgatcatcag agcgaacttt tcagctatta
     9841 actcaagtgg ctggacgtgc tgggcgtgct gaaaaagaag gagaggttgt cattcaaact
     9901 tataatccta atcattatgc tattcaatta gcccaaaagc aagattttga agctttttat
     9961 caatatgaaa tgaatatccg tcgacaactt ggttatcctc cttattattt tacagttggt
    10021 ttaacattat cgcataaaga tgaagagtgg ttaatacgaa aatcttatga agtattaagt
    10081 ttattgaagc aaggctttag tgataaagta aagttgttag gtccaacgcc aaaaccaatt
    10141 gcaagaacac ataaccttta tcactaccaa attattatca agtatcgatt tgaggacaac
    10201 cttgaacttg ttttgaatcg cttattagac atcactcaag acaaggagaa tagagatctt
    10261 cgcttagcaa tagaccacga accgcaaaat atgatgtaga atagtgctat gatataattg
    10321 agaaaagaaa gacgaggaaa aagcaatgac aaaattatta ttcatgggta cccctgattt
    10381 ttcagcgacg gttttaaaag gtattttagc agatggtaag tatgatgttt tagctgttgt
    10441 gacgcaacca gatcgtgctg taggtcgtaa aaaagaaatt aaaatgaccc ctgtaaaaga
    10501 agtggccttg gaaaacaaca ttcctgtgta tcagccggaa aaactatctg gaagtccaga
    10561 attagagcaa ctaatgaccc taggtgcaga tggtattgta acagctgctt ttggtcaatt
    10621 cttaccaaca aagcttttgg aatcggtagg ttttgcaatt aatgtacatg cttctctatt
    10681 accaaaatat cgtggtgggg caccaattca ttatgccatt attaatggcg aaaaagaagc
    10741 tggggttacc atcatggaga tggttgcaaa gatggatgca ggggatatgg tatcaaaagc
    10801 ttccgttgag ataactgatg aagacaatgt agggacaatg tttgatagac tcgccgttgt
    10861 tggtcgtgac cttttattag atacgcttcc aggttatcta tcaggtgata ttaagccgat
    10921 accacaaaat gaagaagagg taagtttttc tcctaatatt agtccagatg aagagagaat
    10981 tgattggaat aaatcatcgc gcgacatttt taaccatgtt cgagggatgt atccatggcc
    11041 agttgcacat acccttttgg agggaaatcg ttttaaactt tatgaagtaa caatgtcaga
    11101 aggaaagggc tcaccgggtc aagtgatcgc taagacaaag aactcattga cagtagcaac
    11161 tggtgacgga gctattgaac tcaaatcagt tcaaccagca gggaaacctc gtatggatat
    11221 aaaagacttt ttaaatggtg ttggtcgtaa tttagaaata ggagataaat ttggcgaatg
    11281 attggaaaaa gagtgcgcgt ggtttagctt taatgacttt agaagaagtt tttgataaag
    11341 gagcttattc taatatcgca cttaataaga gtttaaaaaa atctagacta tcagataaag
    11401 atcgtgcctt agtaacagaa attgtctatg gaactgtggc tagaaaaata acgctagaat
    11461 ggtatctatc acactttata gttgatcgtg ataagcttga attatgggta tatcatctct
    11521 tgctactgag tctttatcaa ctactctatc tggacaacat cccagatcac gctattgtta
    11581 atgatgctgt cactattgct aagaatcgag gcaataaaaa gggtgctgaa aagttaatta
    11641 atgccgtttt acgaagagta agttcggaga cgctccctga aattgcatca ataaaacgcc
    11701 aaaacaaacg ttactctgtt gcttattcca tgccagtgtg gctcgtaaaa aaattaattg
    11761 accaatatgg tgagacaaga gctttagcaa ttatggagag tttatttgag cgaaataaag
    11821 ccagtcttcg tgtgactgac ttatcgcaaa aacaaactat taaagaaact ttaaatgtta
    11881 gagattcaca tattgcagag acagctttag ttgcagactc aggaaatttt gcatcgacat
    11941 catttttcca agatggcctc attactatcc aagatgaatc tagtcaatta gttgctccaa
    12001 cactcaaagt ctcgggtaat gaccaagtat tggatgcatg tagtgcacca ggaggaaaaa
    12061 caagtcatat cgcctcatat ttgactacag gggcagtaac tgctttagat ttgtatgatc
    12121 ataaattgga attggttatg gaaaatgcaa aacgcctagg tctgtcagat aagataaaaa
    12181 ctaaaaagtt agatgcaagt aaagctcatg agtattttcc tgaggatact tttgacaaaa
    12241 ttttagtaga cgcaccttgt tcaggtatcg ggctaattcg ccgtaaacca gatattaagt
    12301 acaataaagc taatcaagac tttgaagccc tccaagagat tcaattgtct atcctatcta
    12361 gcgtttgtca aacgctacgt aaaggtggta taataaccta tagcacttgt accatttttg
    12421 aggaagaaaa ttttcaagtt attgaaaaat tcttagaaaa ccaccccaat tttgagcaag
    12481 tagaactgag tcatacacaa gaggatattg taaagagagg ctgtattagt ataagtcctg
    12541 aacaatatca tacagatggc ttttttattg gacaagtcaa acgtatcttg tagtgtctag
    12601 gaggaaactg acatcgtcag aaaaaaatat atggaaatat ctcttttaac agatattggg
    12661 caacgtcgtt ccaataatca agattttatt aatcaatttg aaaataaagc cggcgtccca
    12721 ctaatcatct tagcagatgg tatgggagga catcgtgcag gaaatattgc tagcgagatg
    12781 actgtaactg atctaggaag tgattgggcg gagacagatt tttctgagtt atcagaaatt
    12841 cgtgattgga tgcttgtctc tattgaaact gagaatcgca aaatatatga attaggtcag
    12901 tcagatgatt acaaaggtat gggaactacc attgaagcag ttgccattgt aggagataat
    12961 attattttcg cacatgtagg agattcacgt atcggtatcg ttcgtcaagg ggagtatcat
    13021 ttattgacga gtgatcattc tcttgttaat gaattggtaa aagccggcca attaacagaa
    13081 gaagaagctg ctagtcatcc tcaaaaaaat attattacgc aatcaattgg tcaagccaat
    13141 ccagtcgaac ctgatttagg agttcatcta cttgaagaag gtgactacct tgtagttaat
    13201 agtgatggac taactaatat gttgagtaat gccgatattg ctacagtact aacacaagaa
    13261 aaaaccttag atgataagaa tcaagattta atcactctag caaaccatag aggaggtctt
    13321 gataatatca ctgtggcact agtttacgtt gaaagtgagg cggtttaatg attcagattg
    13381 gcaaattatt tgctggtcgt tatcggatcc ttaaatcaat cggacgaggt ggaatggcgg
    13441 atgtttatct agctcgagat ctaattttag ataatgaaga ggtcgcaata aaagtattgc
    13501 gaaccaatta tcaaacagat cagattgcgg ttgctcgctt ccagagagaa gcacgagcaa
    13561 tggctgagct gacacatcct aatattgtcg ctattcgtga tataggtgaa gaagacggac
    13621 agcagttctt agtaatggaa tatgttgatg gattcgattt aaaaaagtat attcaagaca
    13681 atgcacccct atcaaataat gaagttgttc gaatcatgaa tgaagtcctt tcagccatgt
    13741 cactggcaca tcagaaaggt atcgtacacc gtgatttaaa acctcaaaat attttattaa
    13801 ctaaaaaagg gactgtaaaa gttacagatt tcggtattgc ggtagcgttt gctgaaacta
    13861 gtttaacgca aacgaattct atgttaggta gtgttcatta tttatcgcca gagcaggcgc
    13921 gtggttcaaa agcgactgtt cagagtgata tttatgccat gggaatcatg ctttttgaaa
    13981 tgttaactgg ccatattcct tatgatgggg atagtgcagt tactattgcc ctacagcact
    14041 tccaaaaacc actaccatca atccttgcag aaaataaaag cgttccacaa gctttggaaa
    14101 acattgtcat taaagcaaca gctaaaaagc taacagatcg ctataagaca acttatgaga
    14161 tgggacgtga tttatcaact gctctaagct cgacacgtca ccgtgaacca aaacttgttt
    14221 ttaatgatac tgagagtact aagacactac caaaagtaac atctacagta tcatctttaa
    14281 cgacagaaca actgttgcgt aatcaaaaac aggccaaaac aacagaaaaa ataactcctg
    14341 attctgcgag taacgataaa acgaagtcta aaaagaaggc ttctcatcga cttcttggta
    14401 caatcatgaa attattcttt gcactctgtg ttgtaggaat aattgtattt gcttataaaa
    14461 ttttagtaag tcctacgact attcgtgttc cagatgttag caacaaaaca gtagctcaag
    14521 caaaaatgac attggaaaat tctggattga aagtaggagc tattcgtaat attgagagtg
    14581 atagtgtttc agaaggcctt gtcgtaaaaa cagaccctgc tgcaggacgt tctcgtagag
    14641 aaggggctaa agttaatctt tatattgcca ctccaaacaa gagtttcact ctaggtaatt
    14701 ataaggaaca taattacaaa gatatcctca aagatcttca aggcaaaggt gttaaaaaat
    14761 ctttaatcaa ggttaagcgt aaaattaata atgattatac tactggtaca atcttagcac
    14821 agtcgcttcc agaaggaaca tcttttaatc ctgatggtaa taagaaatta acacttacgg
    14881 ttgctgttaa tgatccaatg attatgcctg atgtaacagg tatgacagtt ggagaagtaa
    14941 tagaaacctt gacagacctc ggattagatg cagataatct tgtcttctat caaatgcaaa
    15001 atggcgttta tcaagcagta gtaacacctc catcttcaag taagatagca agtcaggatc
    15061 cttattacgg aggagaagtt ggattaagga gaggagataa agttaagctg tatcttcttg
    15121 gttctaaaac aacaaataac tcatcatcta cgccaataga ttcttcggct tcaagctcaa
    15181 caggaacaac tacctcggat tctgtaagct catcaacgga tgctagcact tctgatagta
    15241 gtagcacttc tacaagtagt tcaacactac caagtgatag tacaactaat acaggaacag
    15301 ctaacaatcc attaactcaa taaaattaaa aagaagatgt taaagtcttc tttttaattt
    15361 gctatggtag agaccatgcc tagaaaggtt ctgccaatat gccaaatttt tctctcaata
    15421 atggtgcaaa gtatagggct ttttgataaa atacgtcata tagagtaatg gaagg
//
LOCUS       DATROQ010000041        15396 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.041, whole genome shotgun sequence.
ACCESSION   DATROQ010000041 DATROQ010000000
VERSION     DATROQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15396
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.041"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            150..752
                     /locus_tag="VBC94_001174"
     CDS             150..752
                     /locus_tag="VBC94_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO6979449.1"
                     /translation="MKFLELNKKRHAVKHFNDKPVDFKDVRTAIEIATLAPSANNIQP
                     WKFVVVQEKKSALAEGLPESNCNQINQAQYVIALFTDTDLGQRSRKIARIGRRSLPDD
                     LIGYYMETLPPRYALYSEKQTGEYLSLNAGIVAMNLVLALTDQGISSNMILGFDKAIT
                     NDVLEIDKRFRPEILITVGYSDEKVEPSYRLPVDHIIEKR"
     gene            799..2205
                     /gene="pepV"
                     /locus_tag="VBC94_001175"
     CDS             799..2205
                     /gene="pepV"
                     /locus_tag="VBC94_001175"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEO6979450.1"
                     /translation="MTIDFRAEVDKRKDALMDDLINLLRINSERDDSQADAEHPFGPG
                     PVKALEFFLEMAERDGYETKNVDNYAGHFTFGQGEEELGIFGHLDVVPAGSGWDTDPY
                     EPVIKDNRLYARGSSDDKGPTMACYYALKIIKELGLPTSKKVRFVVGTDEESGWGDMD
                     YYFEHVGLPKPDFGFSPDAEFPIINGEKGNITEYLHFSGENKGAVRLHSFSGGLRENM
                     VPESATARFTSHLDQTTLGASLADFAGKHNLKAELSVEDGQYIATVYGKSAHGSIPQE
                     GVNGATYLALYLSQFDFEGPARAFLDVTANIIHEDFSGEKLGVAYEDDCMGSLSMNAG
                     VFQFDETNDDNTIALNFRYPQGTDAKTIQTKLEKLNGVEKVTLSDHEHTPHYVPMDDE
                     LVSTLLAVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPGDENTMHQANEYMPLE
                     NIFRSAAIYAEAIYELIK"
     gene            2302..2886
                     /locus_tag="VBC94_001176"
     CDS             2302..2886
                     /locus_tag="VBC94_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEO6979451.1"
                     /translation="MTDLEKIIKAIKSDSQNQNYTENGIDPLFAAPKTARINIVGQAP
                     GLKTQEAILYWKDKSGDRLRQWLGVDEETFYHSGKFAVLPLDFYYPGKGKSGDLPPRK
                     GFAEKWHPLILKEMPNVQLTLLVGQYAQKYYLGSSAHKNLTETVKSYKDYLPDYLPLV
                     HPSPRNQIWLKKNPWFEKDLIVDLQKIVADILKD"
     gene            2901..3635
                     /locus_tag="VBC94_001177"
     CDS             2901..3635
                     /locus_tag="VBC94_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979452.1"
                     /translation="MRDNHLHTHFSYDCQTAFEDYINGFTGEFITTEHFDLSNPYTGQ
                     DDVPDYSAYCQKIDYLNQKYGNRFKKGIEIGYFKDRESDILDYLKNKEFDLKLLSIHH
                     NGRYDYLQEEALKVPTKELLADYFNRMEFAIGRVEAHVLDHFDYGFRKLNLDVEDLKP
                     FETQLKRIFRKMLSKGLAFELNTKSLYLYGNEKLYRYALEILKQLGCKQYSIGSDGHI
                     PEHFCYEFDRLQCLLKDYQIDENHLI"
     gene            3804..7562
                     /locus_tag="VBC94_001178"
     CDS             3804..7562
                     /locus_tag="VBC94_001178"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEO6979453.1"
                     /translation="MKRKDLFGDKQTQYTIRKLSVGVASVATGVCIFLHSPQVFAEEV
                     SVSPATTAIAKSNINQVDNRQSTNLKDDINSNSETVVTPSDMPDTKQLVSDETDTQKG
                     VTEPDKATSLLEENKGPVSDKNTLDLKVAPSTLQNTPDKTSQAIGAPSPTLKVANQAP
                     QIENGYFRLHLKELPQGHPVESTGLWIWGDVDQPSSNWPNGAIPMTNAKKDDYGYYVD
                     FKLSEKQRKQISFLINNKAGTNLSGDHHIPLLRPEMNQVWIDEKYGIHTYQPLKEGYV
                     RINYLSSSGNYDHLSAWLFKDVATPSTTWPDGSNFVNQGLYGRYIDVPLKTNAKEIGF
                     LILDESKTGDAVKVQPNDYVFRDLANHNQIFVKDKDPKVYNNPYYIDQVQLKDAQQTD
                     LTSIQASFTTLDGVDKTEILKELKVTDKNQNAIQISDITLDTSKSLLIIKGDFNPKQG
                     HFNISYNGNNVTTRQSWEFKDQLYAYSGNLGAVLNQDGSKVEASLWSPSADSVTMIIY
                     DKDNQNRVVATTPLVKNNKGVWQTILDTKLGIKNYTGYYYLYEIKRGKDKVKILDPYA
                     KSLAEWDSNTVNDDIKTAKAAFVNPSQLGPQNLSFAKIANFKGRQDAVIYEAHVRDFT
                     SDQSLDGKLKNQLGTFAAFSEKLDYLQKLGVTHIQLLPVLSYFYVNEMDKSRSTAYTS
                     SDNNYNWGYDPQSYFALSGMYSEKPKDPSARIAELKQLIHDIHKRGMGVILDVVYNHT
                     AKTYLFEDIEPNYYHFMNEDGSPRESFGGGRLGTTHAMSRRVLVDSIKYLTSEFKVDG
                     FRFDMMGDHDAAAIELAYKEAKAINPNMIMIGEGWRTFQGDQGKPVKPADQDWMKSTD
                     TVGVFSDDIRNSLKSGFPNEGTPAFITGGPQSLQGIFKNIKAQPGNFEADSPGDVVQY
                     IAAHDNLTLHDVIAKSINKDPKVAEEDIHRRLRLGNVMILTSQGTAFIHSGQEYGRTK
                     RLLNPDYMTKVSDDKLPNKATLIEAVKEYPYFIHDSYDSSDAINHFDWAAATDNNKHP
                     ISTKTQAYTAGLITLRRSTDAFRKLSKAEIDREVSLITEVGQGDIKEKDLVIAYQTID
                     SKGDIYAVFVNADSKARNVLLGEKYKHLLKGQVIVDADQAGIKPISTPRGVHFEKDSL
                     LIDPLTAIVIKVGKVAPSPKEELQADYPKTQSFKGSKTVEKVNRIANKTSITPVVSNK
                     TDSYLTNEANLPKTGDKSSKILSVVGISILASLLALLGLSLKRNRHLK"
     gene            7697..8614
                     /locus_tag="VBC94_001179"
     CDS             7697..8614
                     /locus_tag="VBC94_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEO6979454.1"
                     /translation="MKNWEKPPLVMAGLVLGLLALGNLLEGYGTYFRYCLGLVALVFW
                     IFLIKGILKNKKESRKELSNPLIASVFTTFFMAGMILSTYILLFRSLGIWVAVLSKGV
                     WWLSFIALIIHMAIFSWKYLRHFSMANLFPSWSVLYVGIGVASLTAPISGQFTIGKIV
                     FWYGFIATLVLLPFLFIKAYKIGLPSAVKPNITTICAPMSLITAGYVNSFVSPNRGLL
                     LLLIVMAQFLYFFILFQVPKLLIGDFTPGFSAFTFPLVISATSLKLSIQHLSLPVDIQ
                     GLVHFEIGTTTLIVMIVMVRYIFFLRRTI"
     gene            complement(8656..8832)
                     /locus_tag="VBC94_001180"
     CDS             complement(8656..8832)
                     /locus_tag="VBC94_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEO6979455.1"
                     /translation="MVKKFAFAKGIATGVVATAATLAGAAFAIKKTIIEPEEEKIAFI
                     EENRKKAARKRVSH"
     gene            8943..9833
                     /gene="miaA"
                     /locus_tag="VBC94_001181"
     CDS             8943..9833
                     /gene="miaA"
                     /locus_tag="VBC94_001181"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEO6979456.1"
                     /translation="MRKIKLIAVVGPTAVGKTALGIELAKTFNGEIISGDSQQVYQKL
                     DIGTAKASKEEQEQAYHHLIDVREVNENYSVYDFVKEAKVAIDTIISKGKIPIIVGGT
                     GLYLQSLFEGYHLGGEVNQETLKAYREKLESLSDEDLFEKLTEQSIVIPQVNRRRAIR
                     ALELAKFGNDLQNSESPYDVLLIGLNDDRQVLYDRINRRVDLMIDNGLLDEAKWLYDN
                     YPSVQASRGIGYKELFPYFSKQIPLEEAVDKLKQNTRRFAKRQLTWFRNRMNVEFIMV
                     GEENYQQKIKRKVSDFLSSK"
     gene            9924..11162
                     /gene="hflX"
                     /locus_tag="VBC94_001182"
     CDS             9924..11162
                     /gene="hflX"
                     /locus_tag="VBC94_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEO6979457.1"
                     /translation="MIETKEEQERVILVGVELQDTENFEMSMEELASLAKTAGANVVN
                     HYYQKRDKYDSKSFIGSGKLEEIKAIVEADEIDTVVVNNRLTPRQNSNLEAELGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQISDIERQLKIVEKNRETVRERRVDSTTFKIGLIGYTNAGKSTIMNVL
                     TDDKQYEANELFATLDATTKQIYLQNQFQVTLTDTVGFIQDLPTELVAAFKSTLEESR
                     HVDLLLHVIDASDPNHEEHEKVVMEILKDLDMIDIPRLAIYNKMDVTEQLNATTFPNV
                     RIAAKKQGSRDLLRRLIVDEIRHIFDEFSIRVHQNQAYKLYDLNKIALLDTYTFEEEY
                     ENITGYISPKQKWKLEEFYD"
     gene            11155..11802
                     /locus_tag="VBC94_001183"
     CDS             11155..11802
                     /locus_tag="VBC94_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEO6979458.1"
                     /translation="MIDYIDLALTYGGFTSLDKVYLEKKLDGLSKQQRLDFITPPPSV
                     INAYFAEIYQKQGPEAATDYYFDLSKALGLFPKHLSFDEEKPFIRLNLSGKSFGFAYL
                     NDQEEASVFSEVKEVITPQLLLEIAQIFPQYKVYQDRSGIRMAKIDFDETESQNITPE
                     TSLLGNVLQLKKDIIKITSFNQEELLELVKTKSGEYYYSSQGRESVIYIDTKSTN"
     gene            11844..12773
                     /locus_tag="VBC94_001184"
     CDS             11844..12773
                     /locus_tag="VBC94_001184"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEO6979459.1"
                     /translation="MEIQFLGTGAGQPAKARNVSSLVLKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKVKKIFITHMHGDHVFGLPGFLSSRAFQANEEQTDLDIYGPVGIKSFV
                     MTGLRTSGSRLPYRIHFHEFDESSLGKIMETDKFTVYAEKLDHTIFCMGYRVVQKDLE
                     GTLDAEALKLAGVPFGPLFGKVKNGENVTLEDGREIIAKDYISEPKKGKVITILGDTR
                     KTDASIRLALGADVLVHESTYGKGDERIAKSHGHSTNMQAADIAKQANAKRLLLNHVS
                     ARFMGRDCWQMEEDAKTIFSNTHLVRDLEEVGI"
     gene            12775..13536
                     /locus_tag="VBC94_001185"
     CDS             12775..13536
                     /locus_tag="VBC94_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO6979460.1"
                     /translation="MARTILITGASGGLAQAIINQLPQDDHLIVTGRSREKLEKLYGK
                     RPNTLCLSLDITNDNAVTNMIEKIYVEFGQIDILINNAGFGSFKEFWDYSDEEVKDMF
                     AVNTFATMSIARQIGHKMSLVKSGHIVNIASMAGLIATSKASVYGASKFAVVGFLNAL
                     RLELAEKNVYVTSVNPGPIKTGFFAQADPSGDYLASIGRFVLTPEKVAKKVVSILGKN
                     KRELNLPFILAFAHKYYSLFPKTADYFARKVFNYK"
     gene            13533..>15396
                     /gene="recJ"
                     /locus_tag="VBC94_001186"
     CDS             13533..>15396
                     /gene="recJ"
                     /locus_tag="VBC94_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEO6979461.1"
                     /translation="MISAKYSWVLNNQKPDAGFFEASKKEKISEAVASLIYSRGIKTS
                     AELHHFLQTNLENLHDPYLLNDMDKAVNRIRRAIENNETILVYGDYDADGMTSASIMK
                     EALDMMGAEVQVYLPNRFTDGYGPNQSVYKYFIEQQDVSLIITVDNGVAGHEAIAYAQ
                     NQGVDVVVTDHHSMPADLPCAYAIIHPEHPDANYPFPYLAGCGVAFKVACALLETIPT
                     EMLDLVAIGTIADMVSLTDENRIMVKAGLEVMKDSERIGLQELISLSNIDLKTLNEET
                     IGFKIAPQLNALGRLDDPNPAIELLTGFDDEESQAIAQMIDQKNEERKEIVQTIFDQA
                     MQMLDQTKPVQVLAKENWHPGVLGIVAGRILERTGQPVIVLNIEDGIAKGSARSVEAL
                     DIFQAFDQHRELFIAFGGHSGAAGMTLEESKVGDLSQVLCDYISKKQLDMSQKKTLTI
                     DSELRFDELSLDTVRDFEKLAPFGMDNKKPVFLLKDFKVSQARVMGQNGAHLKLKLEQ
                     DGQALDLVAFNMGSQLQEFQQAQHLELAVTLSVNQWNGATTLQLMLEDARVDGIQLFD
                     IRSKASSLPHGVPILSQEEQSKEVILLTVPDHPQELKQMTQGKQFDAIYFKNEIP"
BASE COUNT         5220 a         2361 c         3035 g         4780 t
ORIGIN      
        1 tcattagata ttgtaccaca agtttgtttg ttttttatgg tagttttcaa accggtttca
       61 cttttttatc aattttgcct tcaaaagctg aaacgcattt taaaaagtga tagaatagta
      121 gggaaaatca aatagaatag gaggttttta tgaaatttct agaattaaat aagaagcgcc
      181 atgctgtgaa acattttaat gataagccag tcgactttaa agatgttaga actgctattg
      241 aaattgcgac attagcgcca tctgctaata atattcagcc ttggaagttt gttgttgtcc
      301 aagaaaaaaa atctgctcta gcagaaggtt taccggaaag taattgtaac caaattaatc
      361 aagcgcagta tgttattgct ttatttacag atacagattt aggacagaga tcacggaaaa
      421 tagctcgtat tggtcgtaga tctcttcctg atgatttaat tggttattat atggaaactc
      481 taccaccacg ctatgcttta tatagtgaaa aacaaactgg agagtatctc tctttaaatg
      541 caggtatcgt tgctatgaat ttagtacttg ccttgacaga tcaaggtatt agttctaata
      601 tgattttagg gttcgataaa gcgattacca atgatgtctt agagatagat aaacgtttta
      661 gaccagaaat attaattact gttggttata gtgatgaaaa ggttgagcct agttatcgtt
      721 tgccagttga tcatattatt gaaaaacgtt aatttttaag cacaaactac ataatatttt
      781 atagaaggag ttgccgtaat gacaattgat tttagagcag aagtagacaa acgcaaagac
      841 gctttgatgg acgatttaat aaatcttctt cgtattaact cggagcgtga tgattcacaa
      901 gctgatgcag agcatccatt tggtcctgga cctgtaaaag ccttggaatt tttccttgag
      961 atggctgagc gagatggcta cgaaacaaaa aacgtagata attatgcggg acactttaca
     1021 tttggtcaag gcgaagaaga actaggtatt tttgggcatt tagatgttgt tcctgcaggc
     1081 agcggttggg atacagaccc ttatgaacca gttatcaagg ataaccgttt atatgctcga
     1141 gggtcatcag acgataaggg accaacaatg gcatgttatt atgctctaaa aattattaaa
     1201 gagcttgggc taccaacatc taagaaagtt cgttttgttg taggaactga tgaagaatct
     1261 ggatggggag atatggatta ctacttcgaa catgttggtc ttccaaaacc tgattttggt
     1321 ttctctccag atgcagaatt tccaattata aatggtgaaa aaggtaatat cactgaatat
     1381 ttacattttt caggagaaaa taagggagct gttagactgc atagcttttc tggcggactt
     1441 cgcgaaaaca tggttccaga atcagccaca gcacgtttca caagtcattt agatcagaca
     1501 acacttggag ctagtctagc tgattttgca ggtaaacata atttgaaagc agaattatct
     1561 gtagaagatg ggcagtatat agctacagtt tatggtaaat ctgctcatgg ttcaatacca
     1621 caagaaggtg ttaatggggc gacttattta gctctttatc taagtcaatt tgattttgaa
     1681 ggtcctgctc gtgctttctt agatgtcaca gccaacatta ttcacgaaga cttctcaggt
     1741 gaaaaacttg gagtagctta tgaagatgac tgtatgggat cgttgagcat gaatgcaggt
     1801 gtcttccagt ttgatgaaac taatgatgat aatactatcg ctcttaattt ccgttaccca
     1861 caagggacag atgctaaaac tatccaaact aagcttgaga aacttaacgg agttgaaaaa
     1921 gtgactcttt ctgaccatga acacacacca cactatgtac ctatggacga tgaattagta
     1981 tcaaccttac tagctgtcta tgaaaagcaa actggtctta aaggacatga acaggttatt
     2041 ggtgggggga catttggtcg cttacttgaa cggggtgttg catacggtgc catgttccca
     2101 ggagatgaaa acactatgca tcaagctaat gagtacatgc ctttagaaaa tattttccgt
     2161 tcggctgcta tctacgcaga agctatctat gaattaatca aataaaataa tccttaaact
     2221 aaatatgtga tcaatgataa agggtggtga agacatgaaa gtgtctttgc ctcttttcat
     2281 aaggttagat ttggagactt tatgactgac ttggaaaaaa ttattaaagc aataaaaagt
     2341 gattcacaga atcaaaatta tacagaaaat ggtattgatc ctttgtttgc tgctcctaaa
     2401 acagctagga tcaatattgt tggacaagca cctggtttaa aaactcaaga agcaatactc
     2461 tattggaaag ataaatctgg agatcgtcta cgccagtggc ttggagttga tgaagagaca
     2521 ttttaccatt ctggaaaatt tgctgttttg cctttagatt tttattaccc aggcaaagga
     2581 aaatcaggag atttaccccc tagaaaaggt tttgcggaga aatggcaccc tcttatttta
     2641 aaagaaatgc ctaatgttca attgaccttg ctagttggtc aatatgctca gaaatattat
     2701 cttggaagct ccgcacataa aaatctaaca gaaacagtta aatcttacaa agactatcta
     2761 cccgattatt tacccctggt tcacccatca ccgcgaaatc aaatttggct aaagaagaat
     2821 ccatggtttg aaaaagatct aatcgttgat ttacaaaaga tagtagcaga tattttaaaa
     2881 gattaaggat aggagttagt atgagagata atcatctaca cacgcatttt tcctatgatt
     2941 gtcaaacggc atttgaggac tatattaatg gttttacagg tgaatttatc acaacagaac
     3001 attttgattt atcaaatcct tacaccggtc aagacgatgt tcctgattat agtgcttatt
     3061 gtcaaaaaat agattatctt aatcagaaat atggaaatcg atttaaaaaa ggaattgaaa
     3121 tcggttattt taaagatagg gaatcagata ttttagatta tttaaaaaat aaagaatttg
     3181 atttaaaact attgtcaatc catcataatg gtaggtatga ttatctgcaa gaagaagctc
     3241 tgaaagtacc aacaaaggag cttttagcag attactttaa tcgtatggaa tttgccatag
     3301 gccgtgtgga agcgcacgtt ttagatcact ttgattatgg ttttcgtaag ttaaacttag
     3361 atgtagaaga tttaaaaccg tttgaaacgc aattgaagcg cattttcaga aagatgttat
     3421 ctaaggggtt agcttttgaa ctaaatacca aatcccttta tctatatggg aatgaaaaac
     3481 tttatcgcta tgctttagag atactaaaac agcttggttg taaacaatac tctataggct
     3541 ctgacggtca tattcctgaa catttttgtt atgaatttga tagacttcaa tgtctgctaa
     3601 aggactatca aattgatgaa aatcatttga tatgaggaaa tttttgataa aaaagctagg
     3661 caatattgct tagctttttt gtaatgtcat tgatagtttt agtgaaaatt tcaaaaaaat
     3721 aaagaaatca cttacttgtt gcaagcgctt gcgtaaattg ttatgatttt attggtaaca
     3781 attcattaaa aaaggagaat gatatgaaaa gaaaagactt atttggtgat aaacaaactc
     3841 aatacacgat tagaaagtta agtgttggag tagcttcagt tgcaacaggg gtatgtattt
     3901 ttcttcatag tccacaggta tttgctgaag aagtaagtgt ttctcctgca actacagcga
     3961 ttgcaaagtc gaatattaat caggttgaca accgacaatc tactaattta aaagatgaca
     4021 taaactcaaa ctctgagacg gttgtgacac cctcagatat gccggatacc aagcaattag
     4081 tatcagatga aactgacact caaaaaggag tgacagagcc ggataaggcg acaagcctgc
     4141 ttgaagaaaa taaaggtcct gtttcagata aaaatacctt agatttaaaa gtggcaccat
     4201 ctacattgca aaatactccc gacaaaactt ctcaagctat aggtgctcca agtccgacct
     4261 tgaaagttgc taatcaagct ccacagattg aaaatggtta ctttaggtta catcttaaag
     4321 aattgcctca aggtcatcct gtagaaagca ctgggctttg gatatgggga gatgttgatc
     4381 aaccgtctag taattggcca aatggtgcta tccctatgac taatgctaag aaagatgatt
     4441 acggttatta tgttgatttt aaattatctg aaaaacaacg aaaacaaata tcttttttaa
     4501 ttaataacaa agcaggaaca aatttaagcg gcgatcatca tattccatta ttacgacctg
     4561 agatgaacca agtttggatt gatgaaaagt acggtataca tacttatcag cccctcaaag
     4621 aagggtatgt ccgtattaac tatttgagtt catctggtaa ctatgaccac ttatcagcat
     4681 ggctctttaa agatgttgca accccctcaa caacttggcc agatggtagt aattttgtga
     4741 atcaaggact atatggaagg tatattgatg taccactgaa aactaatgcc aaagagattg
     4801 gttttctaat cttagatgaa agtaagacag gagatgcagt gaaagttcaa cccaacgact
     4861 atgtttttag agatttagct aaccataacc aaatttttgt aaaagataag gatccaaagg
     4921 tttataataa tccttattac attgatcaag tgcagctaaa ggatgctcaa caaactgatt
     4981 taacaagtat tcaagcaagt tttacaactc tagatggggt agataaaact gaaattttaa
     5041 aagaattgaa agtgacagat aaaaatcaaa atgctataca aatttctgat atcactctcg
     5101 atactagtaa atctctttta ataatcaaag gcgactttaa tcctaaacaa ggtcatttca
     5161 atatatctta taatggtaac aatgtcacga caaggcaatc ttgggaattt aaagaccaac
     5221 tttatgctta tagtggaaat ttaggtgcag ttctcaatca agatggttca aaagttgaag
     5281 ccagcctctg gtcaccgagt gctgatagtg tcactatgat tatttatgac aaagataatc
     5341 aaaacagggt tgtagcgact accccccttg tgaaaaataa taaaggtgtt tggcagacga
     5401 tacttgatac taaattaggt attaaaaact atactggtta ctattatctt tacgaaataa
     5461 aaagaggtaa ggataaggtt aagattttag atccttatgc aaagtcatta gcagagtggg
     5521 atagtaatac tgttaatgac gatataaaaa cggctaaagc agcttttgta aatccaagtc
     5581 aacttggacc tcaaaattta agttttgcta aaattgctaa ttttaaagga agacaagatg
     5641 ctgttatata cgaagcacat gtaagagact tcacttctga tcaatctttg gacggaaaat
     5701 taaaaaatca acttggtacc tttgcagcct tttcagagaa actagattat ttacagaaat
     5761 taggagttac acacattcag cttttaccgg tattgagtta tttttatgtt aatgaaatgg
     5821 ataagtcacg ctcaacagct tacacttcct cagacaataa ttacaattgg ggctatgacc
     5881 cacagagcta ttttgctctt tctggaatgt attcagagaa accaaaagat ccatcagcac
     5941 gtatcgccga attaaaacaa ttaatacatg atattcataa acgtggcatg ggggttatac
     6001 ttgatgtcgt ctataatcac actgcaaaaa cttatctctt tgaggatata gaacctaatt
     6061 attatcactt tatgaatgaa gatggttcac caagagaaag ttttggaggg ggacgtttag
     6121 gaaccactca tgcaatgagt cgtcgtgttt tggttgattc cattaaatat cttacaagtg
     6181 aatttaaagt tgatggtttc cgttttgata tgatgggaga tcatgatgcg gctgcgattg
     6241 aattagctta taaagaagct aaagctatta atcctaatat gattatgatt ggtgagggct
     6301 ggagaacatt ccaaggcgat caaggtaagc cggttaaacc agctgaccaa gattggatga
     6361 agtcaaccga tacagttggc gtcttttcag atgatattcg taatagcttg aaatctggtt
     6421 ttccaaatga aggtactcca gctttcatca caggtggccc acaatcttta caaggtattt
     6481 ttaaaaatat caaagcacaa cctgggaatt ttgaagcaga ttcgccagga gatgtggtgc
     6541 agtatattgc tgcacatgat aaccttacct tgcatgatgt gattgcaaaa tcaattaata
     6601 aagaccctaa ggtagctgaa gaagatattc atagacgtct gcgtttagga aatgtaatga
     6661 ttttaacatc tcaagggaca gcattcattc attctggtca agagtatggt cgtacgaagc
     6721 gtttacttaa ccctgattac atgacaaaag tttcagatga caaattgcct aataaagcaa
     6781 cacttattga agctgttaaa gaatacccat attttattca tgattcatat gattcttcag
     6841 atgccattaa tcattttgat tgggcagcag ccacagataa taacaaacac ccaatttcaa
     6901 cgaaaacaca ggcctataca gcaggtttaa tcacattaag gcgttcaaca gatgctttcc
     6961 ggaaattgag caaagcagaa attgatcgtg aggttagctt gattacagag gtaggtcaag
     7021 gtgatattaa agaaaaagat ttggttattg cttaccaaac aatagattct aaaggcgata
     7081 tttacgcagt atttgttaat gctgatagta aagctagaaa cgttttacta ggtgaaaaat
     7141 ataaacacct tttaaaaggg caagtaattg ttgatgctga tcaagcgggg attaaaccaa
     7201 tctcaactcc tagaggtgtt cattttgaaa aagatagttt gctgattgat ccattaacag
     7261 caattgtgat taaagttggc aaagttgctc ctagccctaa ggaggaattg caagcagatt
     7321 atcccaaaac acaatctttc aagggatcta aaacggtaga aaaagtaaat agaatagcta
     7381 ataagacctc aataactcct gtagtttcta ataagaccga ttcatatctg acaaatgaag
     7441 ctaatttgcc aaaaactgga gataagtcat caaaaatact aagtgtagta ggaataagca
     7501 ttctagcaag tctacttgct ctactaggtc tctctttaaa gaggaatcgt cacttaaaat
     7561 aaaagcaaac tttagaagag cttaaaaaag aagttagtca atgggctagc ttcttttcat
     7621 taagttaata aaatcactta tcagcaaaat ctaattgtgt tataatggat gtaaacgttt
     7681 ttttatgagg tatatgatga aaaattggga aaaaccacca ttagtgatgg cgggattagt
     7741 attggggcta ttagctttgg ggaaccttct agagggatat ggcacctatt ttcgctattg
     7801 tttagggcta gtagcacttg ttttttggat ttttttaatc aaaggaatat taaaaaataa
     7861 aaaagaaagc aggaaggagt taagcaatcc tttgattgct tctgtcttta ctactttttt
     7921 tatggcaggt atgattttat cgacctatat cttattattt agaagtttag gaatatgggt
     7981 agcagttctg tcaaaaggtg tatggtggtt gtcatttatt gcgttaatca ttcatatggc
     8041 tattttttca tggaaatatt tgagacactt ttcgatggct aatctttttc cttcttggtc
     8101 tgtcctctat gttggtatag gggtagctag cctgacagca ccaattagtg gacaattcac
     8161 tattgggaaa atagtttttt ggtatggttt tattgcaact ttagtattat taccatttct
     8221 attcattaaa gcatataaaa tcgggctacc ttcggcagtt aagcctaata taactactat
     8281 ttgcgctcca atgtcgctaa tcacagcggg atatgttaat agttttgtgt caccaaatag
     8341 aggtttatta ttgctattaa tcgtaatggc acaatttctt tattttttca ttctttttca
     8401 ggtgcctaaa ttactgattg gtgattttac accaggtttc tcagccttta ctttccccct
     8461 tgttatctca gctacctcac ttaaattaag tattcagcat ctatcattac cagttgatat
     8521 acaggggcta gtccactttg aaattgggac aacaactctt attgttatga tagtaatggt
     8581 acgttatatt ttctttttaa ggcgaacaat ttaatataaa ataactccct tactagctag
     8641 taagggagtt attgtttaat gagagacacg tttacgtgca gcttttttac gattttcttc
     8701 gataaaagct attttttctt cctcaggttc aataattgtt tttttgatag caaatgcagc
     8761 acctgcaaga gtagcagctg ttgcaacgac tcctgttgct attcctttag caaatgcgaa
     8821 ttttttaacc ataataattc tccaatctat gatgaagact ctgcttctat ctatgatata
     8881 attatggtaa cgaaatacta gttaaatttc aagtaaaaac ctttatagaa ttgagaagta
     8941 tcatgagaaa aatcaagctt atagcagttg ttggtccaac tgcggttggg aagacagcat
     9001 taggaattga attagctaaa actttcaatg gcgaaatcat tagtggcgat agtcagcaag
     9061 tttatcagaa gttagatatc ggaacggcta aagctagtaa agaagagcaa gaacaagctt
     9121 atcatcactt gattgatgtt cgtgaagtaa atgagaatta ttcggtttat gattttgtca
     9181 aagaggcaaa agtagctatt gatacgatta tttctaaggg gaaaatccct attattgttg
     9241 gaggtactgg cctatattta cagagtttgt ttgagggcta tcatttagga ggcgaagtca
     9301 atcaagaaac tcttaaggcc tatcgtgaaa aattagagtc tctaagtgat gaagatttat
     9361 ttgaaaagtt gacagaacaa tccattgtta taccgcaagt taatcgtcga cgtgccatcc
     9421 gtgcattaga attggcgaag tttgggaatg atttgcaaaa tagtgaaagt ccgtatgatg
     9481 ttttattaat tggattgaat gatgatcgtc aggtgctcta tgatcgtatc aatagacgag
     9541 ttgatttgat gatagacaat ggtctcttag atgaagctaa atggctatac gataactacc
     9601 ctagtgttca agcaagtaga ggaattggct ataaagaatt atttccttac ttttcaaagc
     9661 aaataccatt agaagaagct gttgataaac taaaacaaaa taccagacgt tttgcaaaac
     9721 gccaattaac atggtttaga aaccgtatga atgtcgaatt tattatggtt ggtgaggaaa
     9781 attatcaaca aaaaatcaag cgaaaagtca gtgatttttt atcatcaaag taaataaagc
     9841 cgtttctcgc atttaaaggg cattatgtta taataaagat tacgtattta gaacaagaaa
     9901 aaaatattga aaggagatta taggtgatag aaactaaaga agagcaagaa cgtgttattt
     9961 tagttggggt agaacttcaa gatactgaga attttgaaat gtccatggaa gaacttgcta
    10021 gcttagctaa gacagcagga gcaaatgttg ttaatcatta ttatcaaaaa agagataaat
    10081 atgatagtaa atcatttatc ggatctggta aattagagga gataaaagct attgtagaag
    10141 cagatgagat tgataccgtt gtggttaata atcgtttgac tccgcgacaa aactctaatt
    10201 tggaagctga acttggtgtt aaagttattg accgtatgca acttatttta gatatctttg
    10261 ctatgcgtgc tagaagtcat gaaggaaaat tacaggttca tctagcgcaa ttgaaatata
    10321 tgttaccacg cttagttgga caaggtatta tgcttagtcg tcaagcagga ggaatcggaa
    10381 gccgaggtcc aggagaatct cagttagagt tgaatcgtcg ttccattcgt catcaaattt
    10441 ctgatatcga aagacaattg aagattgtag aaaaaaatcg cgagacagtt cgagaaagac
    10501 gtgttgattc gacaaccttt aaaattggat taattggata tacaaatgct ggcaagtcaa
    10561 ctattatgaa tgttttgacc gatgataagc aatatgaagc caatgaatta tttgcaacgt
    10621 tagacgctac aaccaagcag atctatttac aaaatcaatt tcaagtcact ttaacagata
    10681 ctgttggttt tattcaagat ttacctacag agttagttgc ggctttcaag tctaccttag
    10741 aagaaagtcg acatgttgat ttattacttc atgttattga tgctagtgac cctaatcatg
    10801 aagaacacga aaaagttgtt atggagattc ttaaagactt agatatgata gatattcctc
    10861 gtttagcgat atacaacaag atggatgtca ctgagcagct gaatgcaaca acttttccta
    10921 atgtgagaat tgcagccaaa aaacaaggga gtagggattt attacgccgt cttattgtgg
    10981 atgaaatacg acatatcttt gacgaatttt cgattagagt gcatcaaaat caagcatata
    11041 agctctatga tctcaataaa atagcattgc ttgacaccta cacctttgaa gaagaatacg
    11101 agaatattac aggctatatt tctcctaaac aaaaatggaa gctggaagaa ttttatgatt
    11161 gattatattg atttggcttt gacttatggt ggttttacga gcttagataa agtttatcta
    11221 gagaaaaaac tagatggcct ctctaagcaa caacgacttg attttatcac tccacctcct
    11281 agtgtgatta atgcttattt tgctgaaata tatcaaaagc aagggccaga agcggcaact
    11341 gattactatt ttgatctttc caaagccttg ggcttgtttc caaaacattt atcgtttgat
    11401 gaagaaaaac cttttattcg tttgaattta tcaggaaaat catttggttt tgcttaccta
    11461 aatgatcaag aagaagcgag tgttttttct gaagttaaag aagtcattac accgcagtta
    11521 ctcttagaaa ttgcacagat ttttcctcaa tataaagtct atcaagatcg gtctggtatc
    11581 agaatggcaa aaatagattt tgacgaaaca gaatctcaaa atattacacc agaaacaagt
    11641 ttactaggga atgtcttaca acttaaaaaa gatattatta aaataactag tttcaatcaa
    11701 gaagagctat tagagttggt taaaacaaag tcaggtgaat actattattc tagtcaagga
    11761 cgtgagtcag ttatttatat tgatactaaa tctactaatt aaatttatta agttatggaa
    11821 ggattataaa aaagtcaaca agtatggaaa ttcaattttt aggaactgga gctggtcaac
    11881 cagcgaaagc tcgtaatgtt tcaagtttag tcttgaaatt attagatgaa attaacgagg
    11941 tttggatgtt tgattgtgga gagggtacac agcgtcaaat tttagaaacc acgattaaac
    12001 ctcgtaaagt caaaaaaatt tttatcacgc acatgcacgg agaccatgtt tttggtcttc
    12061 caggtttttt atccagtcgg gcttttcaag ccaatgaaga acagacagat ttagatattt
    12121 atggaccggt cggtataaaa agttttgtta tgactggctt aaggacatca ggttcacgtt
    12181 taccttatcg aattcatttt cacgagtttg atgaatcatc actcggaaaa attatggaaa
    12241 cagataaatt taccgtttat gcagaaaagc tagatcatac tatcttttgt atgggatatc
    12301 gtgttgtgca aaaagacctt gagggcactt tagatgctga agcattgaaa ctagcaggtg
    12361 ttccatttgg tccccttttt gggaaagtca aaaatggtga gaatgttacc ttagaagatg
    12421 gtagagaaat tattgctaaa gattatattt ctgaaccgaa aaagggtaaa gtaatcacta
    12481 ttttgggaga tactagaaaa acagatgcaa gtattaggtt agctttgggc gctgacgttt
    12541 tagtgcacga atctacttat ggtaagggtg atgagcgaat tgctaaatcg catgggcatt
    12601 caaccaatat gcaagcagct gatatcgcta aacaagcaaa tgctaaacga ctattattaa
    12661 atcatgttag tgctcgtttt atggggcgag attgttggca aatggaagaa gatgcaaaga
    12721 cgattttttc aaatacccat cttgttagag atttggaaga agtaggtata taaaatggca
    12781 agaacgatac tgattacagg agcttctggt ggtttagcac aggcaattat taaccaatta
    12841 cctcaagacg accatttaat tgtcacaggg agatcaagag aaaaacttga aaagctttat
    12901 ggaaagagac caaacacctt atgtttatca ctagacataa ctaatgacaa tgctgtaacg
    12961 aatatgattg aaaaaattta tgttgaattt ggtcaaattg atattttaat taataatgct
    13021 ggatttggtt cttttaaaga attttgggat tatagtgatg aagaagtcaa agacatgttt
    13081 gctgtgaata catttgcaac gatgtctata gctcgtcaga taggacataa gatgtctctt
    13141 gtaaaatctg gtcatatcgt taatattgca agcatggctg gcctcatagc aacaagtaaa
    13201 gctagcgttt atggggctag taagtttgct gtcgttggat ttttgaatgc tcttcgttta
    13261 gaattagcag aaaaaaatgt ttatgtgaca agtgtaaacc ctggaccaat caaaacagga
    13321 ttttttgctc aggctgatcc aagtggtgat tatttagcaa gtataggtcg ttttgttcta
    13381 acaccagaaa aagtagctaa aaaagtagtt tctattcttg gaaaaaataa gagagaatta
    13441 aatcttcctt ttatattagc ttttgcgcat aaatattata gtttatttcc aaaaacagct
    13501 gattattttg ccagaaaggt attcaattat aaatgatatc tgctaagtat agttgggtcc
    13561 tcaataacca aaagccagac gctggctttt ttgaggcttc aaaaaaagaa aagataagtg
    13621 aagcggtggc ttccttaatt tattcgcgag gtattaagac atcagcggaa ttacatcatt
    13681 ttttacaaac taatttggaa aatctccatg atccttattt gttaaatgat atggataaag
    13741 ctgttaatcg tattagacga gctattgaaa ataatgaaac tattttagtt tatggagact
    13801 atgatgctga tggaatgaca tcagcatcaa tcatgaaaga agcattagat atgatgggag
    13861 ctgaagtcca ggtttatctc ccaaatcgct ttacagatgg ttacggtccc aatcaaagtg
    13921 tttataaata ttttattgaa caacaagatg tttcattaat cattacggtt gataatggtg
    13981 tggcagggca tgaagcaatt gcatatgctc aaaatcaagg tgtggacgtg gtagttactg
    14041 atcatcacag tatgccagca gatttacctt gtgcttatgc tattattcac ccagaacatc
    14101 ctgatgctaa ttatccattt ccttatttag cagggtgtgg tgttgctttc aaagtagctt
    14161 gtgctctctt agaaacaatc ccgacagaaa tgttggattt ggttgctatt gggacgattg
    14221 cagatatggt tagcctaact gatgaaaatc gtatcatggt aaaagctgga cttgaagtta
    14281 tgaaagatag tgaaagaata ggcttacagg agctaatatc tttatcaaat attgatttaa
    14341 aaacactaaa tgaagaaact attggattta aaattgctcc acaattaaac gctttaggaa
    14401 gacttgatga ccctaatcca gctattgaat tactaacagg gtttgatgat gaagaatctc
    14461 aagcaatagc ccaaatgatt gatcaaaaaa atgaagagcg caaagaaatc gttcaaacta
    14521 tctttgacca agctatgcaa atgcttgatc aaacaaagcc tgtacaagtt ttagcaaaag
    14581 aaaattggca tccaggggtt ttaggtattg tggcgggtcg tattttagag agaactggac
    14641 aacctgttat tgttttgaat attgaagatg gtattgctaa aggaagtgcc agaagtgtag
    14701 aagcacttga tatttttcaa gcttttgacc agcatcgaga attattcatt gcttttggag
    14761 gtcacagcgg agcagcaggc atgactttag aggaaagtaa ggtaggtgat ctttctcaag
    14821 ttttatgtga ctatatctct aaaaaacaat tggacatgag tcagaaaaaa actctgacaa
    14881 ttgatagtga gctacgtttt gacgaactta gtttagacac tgttagagat tttgaaaagt
    14941 tagcgccatt tggaatggat aataaaaaac cagtcttttt gttaaaagat tttaaagtga
    15001 gtcaagccag agtcatgggg caaaacggtg ctcatctgaa attaaaacta gaacaagatg
    15061 gacaagcact tgatttggta gcatttaata tggggagtca actacaagag tttcaacaag
    15121 cacaacattt ggagttagct gtcactttat ctgttaatca atggaatggt gccacaaccc
    15181 tacaattaat gttggaggat gcgcgtgtag atggtattca attatttgat attagaagca
    15241 aagcaagtag cttacctcat ggagtgccaa tcctgtctca agaagagcag agcaaagaag
    15301 ttattctttt gactgttccc gatcatccac aagaattaaa acaaatgact caaggtaaac
    15361 agtttgatgc tatctatttt aaaaatgaga taccaa
//
LOCUS       DATROQ010000042        15114 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.042, whole genome shotgun sequence.
ACCESSION   DATROQ010000042 DATROQ010000000
VERSION     DATROQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15114
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.042"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            4..837
                     /locus_tag="VBC94_001187"
     CDS             4..837
                     /locus_tag="VBC94_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEO6979462.1"
                     /translation="MLATVLLTLSGCANWIDKGQSITSVGSTALQPLVEAAADEFGKT
                     NLGKTINVQGGGSGTGLSQVQSGAVQIGNSDLFAEEKDGIDAKKLKDHQVAVAGLAVI
                     VNKKVNVKNLTTHQLRDIFAGKIKNWKEVGGQDLDISIINRAASSGSRATFDNTIMGN
                     VAPIQSQEQDSNGMVKSIVSQTPGAISYLAFAYVDKSVGTLKLNGFAPTAKNVTTDNW
                     KLWSYEHMYTKGNETGLTKEFLDYMKSDKVQSSIVQHMGYISINDMKVVKDAEGKVTP
                     K"
     gene            883..1800
                     /gene="pstC"
                     /locus_tag="VBC94_001188"
     CDS             883..1800
                     /gene="pstC"
                     /locus_tag="VBC94_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO6979463.1"
                     /translation="MKNQELAKKLTSPSKNSRLEKFGKGITFLSLALIVFIVAMILIF
                     VAQKGLSTFFVDGVKLTDFLFNTKWEPSAKSFGALPMIAGSFIVTILSAIIATPFAIG
                     AAVFMTEISPKYGSKILQPAVELLVGIPSVVYGFIGLQIIVPFVRSIFGGTGFGILSG
                     VCVLFVMILPTVTFMTVDSLRAVPRHYKEASLAMGATRWQTIWRVILNAARPGIFTAI
                     VFGMARAFGEALAIQMVVGNSAILPTSLTTPAATLTSVLTMGIGNTVMGTVQNNVLWS
                     LALVLLIMSLAFNTVIKLITREGKKNYER"
     gene            1790..2677
                     /gene="pstA"
                     /locus_tag="VBC94_001189"
     CDS             1790..2677
                     /gene="pstA"
                     /locus_tag="VBC94_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO6979464.1"
                     /translation="MNAKKADKLATTILYSIAAIIVTILASLLIFILVRGLPHVSWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSLGAGIYLSEYAKKGRLTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFQYGFSIISGALALTVFNLPQMTRSVEDSLQNVH
                     HTQREAGLALGISRWETVIYVVVPEALPSIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNVLSVTSPISIFRQAETLAVHIWKVNSEGTIPDATQVSAGSAAVLLVVILIF
                     NLSARSIGKKLHSKLTSSN"
     gene            2689..3492
                     /gene="pstB"
                     /locus_tag="VBC94_001190"
     CDS             2689..3492
                     /gene="pstB"
                     /locus_tag="VBC94_001190"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6979465.1"
                     /translation="MAEYNWDERHIITFPEENSALTTKDLHVYYGEKEAIKGIDMQFE
                     KNKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGQIMYEGIDVNAQDINVYEMRKH
                     IGMVFQRPNPFAKSIYKNITFAYERAGVKDKKFLDEVVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAIAVKPEILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGDLIEYDKTNNIFQNAKCQSTSDYVSGRFG"
     gene            3504..4262
                     /gene="pstB"
                     /locus_tag="VBC94_001191"
     CDS             3504..4262
                     /gene="pstB"
                     /locus_tag="VBC94_001191"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6979466.1"
                     /translation="MTQPILQVSDLSVYYNKKKALKEVSMDFYPNEITALIGPSGSGK
                     STLLRAINRMGDLNPEVTLTGAVMYNGHNVYSPRTDTVELRKEIGMVFQQPNPFPMSV
                     FENVVYGLRLKGIKDKATLDEAVETSLKGASIWDEVKDRLHDSALGLSGGQQQRVCIA
                     RTLATKPKIILLDEPTSALDPISAGKIEETLHGLKDQYTMLLVTRSMQQASRISDRTG
                     FFLDGNLIEYGNTKEMFMNPKHKETEDYITGKFG"
     gene            4296..4949
                     /gene="phoU"
                     /locus_tag="VBC94_001192"
     CDS             4296..4949
                     /gene="phoU"
                     /locus_tag="VBC94_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO6979467.1"
                     /translation="MLRSKFDEELDKLHNQFYAMGIEAIGQIKKTVRAFVSHDRELAK
                     EVIEDDVTLNNFETKLEKKSLEIIALQQPVSQDLRTVITVLKATSDVERMGDHAAAVA
                     KATIRMKGEERIPAVELEINNMGKAVKNMLEEALTAYINGDDEKAYEVAAMDEIVDDY
                     FRDIQKMVVETIQKHPDVAFAAKEYFQVLMHLERIGDYGKNICEWIVYLKTGKIIEL"
     gene            5095..7644
                     /locus_tag="VBC94_001193"
     CDS             5095..7644
                     /locus_tag="VBC94_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEO6979468.1"
                     /translation="MKTVEHFVTKFVPENYNLFLDINRQTKTFSGNVAVSGEALDNNI
                     SFHQKGLTIKSVLLDNQPLDFQLDEDNEAMHIQLHETGSMVLVFEFSGHITDNMTGMY
                     PSYYTVNGIKKEVISTQFESHFAREVFPSIDEPEAKATFDLSLKFDQKEGEIALSNMP
                     EINAEQRQETGLWTFDTTPKMSSYLLAFALGELHGKTTHTKNGTLVGSYATKAHQLNE
                     LDFSLDIVVRVIEFYEDYFGVRYPIPQSLHVALPDFSAGAMENWGLVTYREVYLLVDE
                     NSSVSSRQQVALVVAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIDYIEPKLN
                     IFEDFQTGGLPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRW
                     LGDTDFAAGLKIYFEKHQYQNTIGRDLWNALSQTSGKDVAAFMDSWLEQPGYPVMAAK
                     IEEDELILTQKQFFIGEHEDKSRLWQIPLNSNWEGIPEILTEETVVIPNFSQLAEKNK
                     ENGALRFNTENTAHYITNYQGQLLEHIISDLPLMDNISKLQIVQERHLLAESGMISYS
                     SLIPLVSLLSQETSYLVNSAIKSVIDGLSLFVQEDSQDEFDFKEFVNKLSAFNFNRLG
                     FEKREGEGDDSEMVRHLSLSLALYSDNEHAIEEAHHIFKAHENNIAAIPAAIRLLVLT
                     NEMKHFESKELSHLLLETYSTTTDGNFKRQLASALSHTTDSKTLKKLLSDWKNKDIVK
                     PQDLAMSWYATFLKNSFTQESVWEWAQENWEWIKATLGGDMSFDKFVIYPSSSFKTEE
                     RLEQYKNFFEPQLSDMAISRNISMGIKEISARVLLITKQKEEVINTIKKYI"
     gene            7806..8486
                     /locus_tag="VBC94_001194"
     CDS             7806..8486
                     /locus_tag="VBC94_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6979469.1"
                     /translation="MIKILLIEDDLSLSNSVFDFLDDFADVMQIFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESIDDKGQGFDLGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNDNSLIYGDIRVDMSTNSTFVNQTEVELLGKEFDLLVY
                     FLQNQNVILPKSQIFDRIWGFDSDTTISVVEVYVSKVRKKLKGTLFSENLQTLRSVGY
                     ILKHVETD"
     gene            8470..9783
                     /locus_tag="VBC94_001195"
     CDS             8470..9783
                     /locus_tag="VBC94_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6979470.1"
                     /translation="MSKPIKISKFKKNVSDSHFIHFFTVFSGIFLVMTVIILQVMRYG
                     VYSSVDSSLKYISTHPKNYINMVMSRTAAYLDNSNIASVKLKPGGQTVANTDIILFTS
                     EEEVINYFDAFSNYQFLKPNKKNLGGISELTLTNIFGQDETYHAVTVKVNNPAYPNVT
                     YMTAIVNIDQLVNAKERYEKIIIFVMTTFWIISIGASIYLAKWAQKPIIENYERQKAF
                     VENASHELRTPLAVLQNRLETLFRKPNATILENSENIASSLDEVRNMRILTTNLLNLA
                     RRDDGIKPELAVIKPTLFDSIFENYDLIAQENGKNFTGHNMIQDSFKTDKTLLKQLMT
                     ILFDNAIKYTDNDGSIDFTISETDKYLFLEIADNGPGISEEDKVRIFDRFYRVDKART
                     RQQGGFGLGLSLAQQIVNSLRGNITVIDNKPRGSIFKIKLPKVKG"
     gene            9872..10204
                     /locus_tag="VBC94_001196"
     CDS             9872..10204
                     /locus_tag="VBC94_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEO6979471.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     SGVSRQAVYDNIKRTEKILEAYEMKLHMYSDYIVRSQIFDDILEKYTDDAFLQEKISI
                     LSSIDNRD"
     gene            10222..11787
                     /gene="ffh"
                     /locus_tag="VBC94_001197"
     CDS             10222..11787
                     /gene="ffh"
                     /locus_tag="VBC94_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEO6979472.1"
                     /translation="MAFESLTERLQGVFKNIRGKKKLSEKDVQEVTKEIRLALLEADV
                     ALPVVKTFIKHVRERAVGHEIIDTLDPTQQIVKIVNEELTDLLGAETSEIEKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEDNARPMMIAADIYRPAAIDQLKTLGSQINVPV
                     FDMGTNHSAVEIVTKGLEQARENRNDYVLIDTAGRLQIDATLMQELHDVKAIAQPNEI
                     LLVVDSMIGQEAANVAEEFNRQLSISGVVLTKIDGDTRGGAALSVREITGKPIKFTGT
                     GEKITDIETFHPDRMASRILGMGDLLTLIERASQEYDEKRSMELAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMLPGMANNPAMKNFKVDENEIARKRAIVSSMTPEERENPN
                     LLNPSRRRRIAAGSGNTFVDVNKFIKDFNQAKQMMQGVMSGDMNKMMKKMGIDPNNLP
                     KDMPGMDGMDMSNLEGMMGQNGMPDLSSLGGDMDFSQMFGGGLKGKVGAFAAKQSMKR
                     MANKMKKAKKKRK"
     gene            11854..12540
                     /locus_tag="VBC94_001198"
     CDS             11854..12540
                     /locus_tag="VBC94_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEO6979473.1"
                     /translation="MYLALIGDIINSKQILERETFQQSFQQLMTELSDVYGEELISPF
                     TITAGDEFQALLKQSKKVFQIIDHIQLALKPVNVRFGLGTGNIITSINSNESIGADGP
                     AYWHARSAINHIHDKNDYGTVQVAICLDDEDQNLELTLNSLISAGDFIKSKWTTNHFQ
                     MLEHLILQDNYQEQFQHQKLAQLENIEPSALTKRLKASGLKIYLRTRTQAADLLVKSC
                     TQTKGGSYDF"
     gene            12530..13303
                     /locus_tag="VBC94_001199"
     CDS             12530..13303
                     /locus_tag="VBC94_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEO6979474.1"
                     /translation="MISDFLRDNPILTLLFCAHFLADFQWQSQSLADSKSHSWRGLWR
                     HLLIVFLPLAALMILIPETTLLNLSIWGSHIVIDSIKKLSYPWVEEGHFQKAAFIIDQ
                     LAHYTCIIVFYHALPTYLPPNHWLLPIKHFIVIALVFIIITKPINIVFKIFFNKFQAK
                     ELSSLLTQEKTKIMKEKSEDHEETIEGAGAMIGNLERLIMAILLISGQYAAIGLVFTA
                     KSIARYDKISKSQVFAEYYLIGSLFSIISVLITHWLLLV"
     gene            complement(13336..14931)
                     /locus_tag="VBC94_001200"
     CDS             complement(13336..14931)
                     /locus_tag="VBC94_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6979475.1"
                     /translation="MSSFNRKKLKFLGISLATLTATTVTLVACGNESKNSGDNKVINW
                     YIPTEISTLDISKNTDAYSNLAIGNSGSNLLRIDKEGKPKPDLAKKVSVSSDGLTYTA
                     TLRDNLKWSDGSKLSAEDFVYTWRRIVDPKTASEYAYLATESHLLNADKINSGDIKDL
                     NKLGVTAKGNQVTFKLTSPCPQFKYYLAFSNFMPQKQSYVEKVGKDYGTTSKNQIYSG
                     PYLVKDWNGSNGKFKLVKNKYYWDSKHVKTNSVIVQTIKKPDTAVQMYKQGQIDFAEI
                     SGTSAIYNQTGSVKALTNQKIRQALNLATDRKGVVKAAVDTGSTPAESLVPKKLAKLP
                     NGEDLSKYTAPGYTYNTSKAQKLFKEGLAEVGQSSLKLTITADSDSPAAKNAVDYVKS
                     TWESALPGLTVEEKFVTFKQRLEDAKNENFDVVLFSWGGDYPEGSTFYGLFTTNSAYN
                     YGKFSSKEYDNAYQKAITTDALKPGDAANDYKTAEKALFDQSYYNPVYYLGKKGLQNS
                     KLKGLVRNSTGLNVDFTYAYKTE"
BASE COUNT         5015 a         2629 c         2753 g         4717 t
ORIGIN      
        1 accttgctag ccaccgtctt attgacgctg tctggttgtg ctaattggat tgataaaggt
       61 caatcaatca ccagcgttgg ttcaaccgcc ttacagccac ttgtagaagc tgcagcagat
      121 gagtttggta agacaaacct cggtaagact atcaatgtcc aaggaggagg ctcaggaaca
      181 ggcttatcac aagtacagtc tggagctgtt caaattggta atagcgactt atttgcagaa
      241 gaaaaagatg gtattgatgc taagaaattg aaagaccatc aagtcgcagt agctggatta
      301 gctgttattg ttaataaaaa agttaatgtt aaaaatctaa ccacccacca acttcgtgat
      361 atttttgcag gaaaaatcaa gaattggaaa gaagtgggag gacaagattt agatatttcc
      421 atcatcaatc gagcagcaag ttctggatcc cgtgcaacct tcgataatac tattatggga
      481 aatgttgctc ctattcaaag ccaagaacaa gattcaaatg gtatggtgaa atcaattgtt
      541 tctcaaacac ctggtgcgat ttcatacctc gcttttgcat acgttgataa atctgtcgga
      601 acattaaaac taaatggttt tgcgccaaca gctaaaaatg ttactaccga caactggaaa
      661 ctttggtcat atgaacatat gtatacaaaa ggtaatgaaa caggtctaac aaaagaattc
      721 ttagactaca tgaaaagtga taaagttcaa agtagtatcg tacaacatat gggatatatc
      781 tctattaacg acatgaaagt tgttaaagat gctgaaggta aagtaacccc aaaataaaac
      841 ttaatcacat ctagataaag catcttgatc ctaggaggaa ttatgaaaaa tcaagaatta
      901 gctaaaaaat taacatctcc ttcaaaaaac tctcgattag aaaaatttgg taaaggaata
      961 acttttcttt cgcttgccct aatcgtcttc attgttgcga tgattttgat ttttgttgct
     1021 caaaaaggac tatctacatt ttttgttgat ggcgttaaat taacagactt cttatttaat
     1081 acaaaatggg aaccaagtgc taagtcattc ggtgctcttc caatgatagc tggatcattt
     1141 attgttacta ttctatcagc tatcattgct acaccatttg ctatcggtgc tgccgtattt
     1201 atgactgaaa tctcacctaa atatggttct aaaattttac aacctgcagt tgaattacta
     1261 gttgggattc cttctgttgt ttacggtttt attggtttac agattattgt tcccttcgtt
     1321 cgctctattt ttgggggaac aggttttggc attttatctg gggtctgtgt cctcttcgtc
     1381 atgattctac caactgttac tttcatgaca gtagatagcc ttagagctgt gccacgtcac
     1441 tataaagaag caagtttagc catgggggca actcgttggc aaacaatttg gcgtgttata
     1501 ttaaacgctg cacgtccagg aattttcact gcaattgttt ttggtatggc acgtgcattt
     1561 ggtgaagctc tagcaattca gatggttgtc ggaaactctg caattttacc aacatcattg
     1621 acaacaccag cagcaacatt aacgtctgtt ctaacaatgg gtattggtaa tactgtaatg
     1681 ggaactgtac aaaacaatgt cctctggtcg ctagccttag tacttttaat tatgagttta
     1741 gcatttaata cagttattaa gttaattaca agggaaggaa agaaaaacta tgaacgctaa
     1801 aaaggctgac aaattagcaa ctacaatact ctatagtatt gcagctatca tcgttacaat
     1861 cctcgcctct cttttaattt ttattcttgt tcgcggattg ccacacgtat cttggtcatt
     1921 tttaactggg aaatcatcat catatgaagc aggaggcggt attggaatac aattatacaa
     1981 ttcctttttc ctacttattg ttactctgat tatttctatt cctctatctt taggagctgg
     2041 aatatacctc tcagaatatg ctaaaaaagg acgtttaaca aactttgtta gaacatgtat
     2101 cgaaattttg tcatctcttc cttctgttgt agttggtctg tttggttacc taatcttcgt
     2161 tgttcaattt caatatggct tctcaattat ctcaggtgcc ttagcactta ctgtttttaa
     2221 cttaccccaa atgacacgta gtgttgaaga tagccttcaa aatgtacacc acacacagcg
     2281 tgaagcagga ttggccctag gtatttctcg ttgggaaacg gtcatttacg tcgttgtccc
     2341 tgaagctctg ccaagtattg taactggtgt tgtgctagct tcaggacgta ttttcggtga
     2401 ggcagcagca cttatttata cagctggtca atctgctcca gcacttgatt ggtcaaattg
     2461 gaacgtcctt agtgttacaa gtccaatctc aattttccgt caagcagaaa ctttagctgt
     2521 tcatatttgg aaagttaata gcgaagggac aatccctgat gcaacacaag tatctgccgg
     2581 tagcgccgca gtcttattgg ttgttattct tattttcaac ctttcagctc gctcgatcgg
     2641 taagaaactt cattcgaaat taacctcgtc taattaaaag gagtagtaat ggcagaatat
     2701 aattgggacg aacgccatat cattaccttc cctgaagaaa attcagctct caccacaaaa
     2761 gatttacacg tttactacgg tgagaaagaa gctatcaagg gaattgatat gcaatttgaa
     2821 aaaaataaaa ttacagcctt aatcggccca tctggatgtg gaaaatcaac atatcttcgt
     2881 agcttaaatc gtatgaacga tacgattgat attgcacgcg tgactggaca aatcatgtat
     2941 gaaggaattg atgttaacgc tcaagatatc aatgtttacg agatgcgaaa acatatcgga
     3001 atggttttcc aacgtccaaa cccgtttgca aaatcaattt ataaaaacat tacctttgca
     3061 tatgagcgtg ctggtgtaaa agataaaaaa ttcttagatg aagttgtcga aacctcacta
     3121 aaacaagctg ctctatggga tcaagtaaaa gatgaccttc ataaatcagc attcacttta
     3181 tctggcggtc aacagcagcg attatgtatt gcacgtgcta tcgcagttaa accagaaatt
     3241 ctcttaatgg atgaacctgc ttcagcactc gatccgattg caactatgca actagaagag
     3301 acaatgtttg aactcaagaa aaattacact ataattatcg taacacataa tatgcaacaa
     3361 gcagcacgtg ctagtgatta tactgcattt ttctatcttg gagacttaat cgaatacgat
     3421 aaaacgaata acattttcca aaatgctaaa tgtcaatcta ctagcgatta tgtttctggt
     3481 cgatttggat agtgaggtaa aagatgacac aacctatttt acaagtcagt gatttgtctg
     3541 tttactataa taagaaaaaa gctttaaaag aagtctcaat ggacttctat cctaatgaaa
     3601 ttacagcctt aattggtccg tctggttctg gaaagtcaac actcttacga gctattaacc
     3661 gtatgggaga cttaaatcct gaagtcactc taacaggagc agtgatgtat aacggacata
     3721 atgtttatag cccgcgcact gataccgtag aacttcgtaa agaaattggg atggtttttc
     3781 agcagcctaa tccatttcct atgtcagtat ttgaaaatgt tgtttatggt cttagactca
     3841 aaggaataaa agacaaagct acgttagatg aagccgtaga gacttcattg aagggcgcgt
     3901 ctatctggga cgaggtcaaa gatagattac acgactctgc tcttggttta tctggtggtc
     3961 aacaacaacg tgtttgtatt gccagaaccc ttgctaccaa gcctaaaatt atccttctag
     4021 atgagccaac ctctgcactt gacccaattt ctgctggtaa aattgaagag actttacatg
     4081 gtctaaaaga tcaatatacc atgttacttg ttacacgttc tatgcagcaa gcctcacgta
     4141 tttctgatcg tacaggtttc ttcttagacg gtaacttaat tgaatatggc aatacaaaag
     4201 aaatgtttat gaatcctaaa cataaagaaa ctgaagatta cattacaggt aaattcggtt
     4261 aataccatat aataagatca gaaagagaat acaatatgtt aagatcaaaa tttgatgaag
     4321 aattagataa actgcataac caattctatg caatgggaat tgaagctatc ggacaaatca
     4381 aaaaaacagt tcgtgctttt gtcagtcatg atcgagagtt agctaaagaa gtgatagaag
     4441 atgatgtaac gcttaacaac tttgaaacga agttagagaa aaaatcattg gaaattattg
     4501 cactccagca gcctgtatca caagacttac gcaccgttat taccgtttta aaagctacta
     4561 gtgatgttga gcgtatggga gaccatgcag cagcagttgc aaaagcaacc atccgtatga
     4621 agggcgagga aagaatccca gcagtagaat tagaaatcaa taatatggga aaagcagtta
     4681 aaaacatgct tgaagaagcc ttaacagctt atatcaatgg tgatgatgaa aaagcttacg
     4741 aagtagcagc aatggatgaa atagttgatg attacttccg tgatatccaa aaaatggtag
     4801 ttgaaacaat tcaaaagcat cctgacgtag catttgccgc caaagaatac ttccaagtat
     4861 tgatgcactt ggagcgaatt ggagattatg gcaaaaatat ctgtgaatgg attgtttatt
     4921 taaaaactgg aaaaattatc gagttatgat ataattattg cttgtagagt ggtaattgta
     4981 agtatagttc gtgcgataga aaagctgtaa ctatcttgtt acagcttttc tatcttttct
     5041 agcccataca ggactagtaa tgtttcaaat gtttctgaat tagttaggag ttatatgaaa
     5101 actgtagaac attttgttac caaatttgtc cctgaaaact ataacctttt cttggatatt
     5161 aatcgtcaaa ctaaaacctt ttcaggtaac gttgctgtgt ctggcgaagc cttagataac
     5221 aatatctctt ttcaccaaaa agggttaacc attaaatctg tcttattaga taatcaacca
     5281 ttagatttcc aactagatga agacaatgaa gctatgcata ttcaattgca tgaaactggt
     5341 agcatggtat tagtctttga attcagcggt cacattaccg ataatatgac aggtatgtat
     5401 ccatcttact atacagttaa tggcatcaaa aaagaggtta tctccacaca atttgaaagt
     5461 cattttgctc gtgaagtatt cccaagtatt gacgaaccag aagcaaaagc aacctttgat
     5521 ttgagcctaa aattcgatca aaaagaagga gagattgctc tttcaaatat gccggaaatc
     5581 aatgctgaac aacgtcaaga aacaggttta tggacatttg acaccacacc taaaatgtcc
     5641 tcatatttat tagcttttgc cttgggggaa ttgcatggta aaacaacaca taccaaaaac
     5701 ggcactcttg ttggctcata tgccacaaaa gcccatcagt taaatgaatt agatttttct
     5761 ttagatatcg ttgtgcgtgt tattgagttt tatgaggact actttggtgt acgttatcct
     5821 attcctcagt cactccacgt tgctctacct gatttttctg ctggagctat ggaaaattgg
     5881 ggattagtaa catatcgtga agtctatctt ttagtagatg agaactctag tgtttccagc
     5941 cgtcagcaag tagcattagt tgtagcacat gaaatagctc accagtggtt tggtaacctc
     6001 gttactatga aatggtggga tgatttatgg ttaaatgaat catttgctaa tatgatggaa
     6061 tatgtatcaa ttgattatat tgaaccaaaa cttaatatat ttgaagactt tcaaacaggt
     6121 ggtctaccat tagctcttaa gcgtgatgcc acagatggcg tccaatctgt tcacgttgag
     6181 gtcaatcatc ctgatgaaat taacacactt tttgatccag ctatcgttta tgcaaaaggt
     6241 agccgcctca tgcacatgtt acgtcgctgg ttaggcgata cagattttgc cgcaggacta
     6301 aaaatttatt ttgaaaaaca tcaatatcaa aatactattg gtcgtgacct ttggaatgca
     6361 ctttctcaaa cctcaggaaa agatgttgca gctttcatgg attcttggtt agagcaacca
     6421 ggataccctg tgatggcagc caaaatagaa gaagatgagc tcattttaac tcaaaaacaa
     6481 ttctttattg gtgaacacga agataagtct cgcttgtggc aaattcctct taatagtaat
     6541 tgggaaggaa tcccagagat attaaccgag gaaacggtag taatccccaa ttttagtcag
     6601 ttagctgaaa agaacaaaga aaatggtgca ctgcgtttca atactgaaaa tactgcacat
     6661 tacattacta actatcaagg tcaactctta gagcatatca tttctgatct tcctcttatg
     6721 gataacattt ctaaactaca aattgtacaa gaacgtcatt tacttgctga aagtggtatg
     6781 atttcatata gtagtttaat acctttagtc tctcttttga gccaagagac atcatatctt
     6841 gttaactctg ctattaagag tgttattgat ggtttatcct tattcgtcca ggaagatagc
     6901 caagatgaat tcgactttaa agaatttgtt aataaattat cagcatttaa ttttaataga
     6961 cttggctttg aaaaaagaga aggagaagga gatgatagcg aaatggtccg tcatctatcc
     7021 ctaagccttg ctctctactc agataatgaa catgctattg aagaagctca ccatattttt
     7081 aaagcccacg aaaataatat cgctgctata ccagctgcaa ttcgcctgtt agttcttacc
     7141 aatgaaatga agcatttcga atccaaagaa cttagccact tgttattaga gacttatagt
     7201 actactaccg atgggaattt caaacgacaa ctagcgagtg ctttatctca tactacagat
     7261 agcaagacac tcaagaaatt attgtcagat tggaaaaata aagatattgt gaaaccacaa
     7321 gacttagcta tgagttggta tgctactttc cttaaaaatt cttttacaca agaaagtgtc
     7381 tgggagtggg cccaagaaaa ctgggaatgg atcaaagcaa ctctaggtgg tgatatgagt
     7441 tttgataaat ttgtgattta tccctcaagt agtttcaaaa cagaagaacg ccttgaacaa
     7501 tataaaaact ttttcgagcc tcaattaagc gatatggcga ttagccgtaa tatttcaatg
     7561 ggaattaaag aaatttctgc tcgtgtcctt ttaatcacga agcaaaaaga agaagttatc
     7621 aatactatca aaaaatatat ctaataaact caaatagaga ttgatggatt attcaatctc
     7681 tatttctttg tctgtgtcat gaaattagtt gacataaaac atactttatt taggatttat
     7741 taagcaaagt ttggtattct aatattaata gttcagataa aatatgtaca tcgaaaggat
     7801 atactatgat aaaaatttta ttaattgaag atgacttgag tctttccaat tccgtttttg
     7861 atttccttga tgactttgcc gatgtcatgc aaatttttga tggagaagag ggtctttacg
     7921 aagccgaaag tggagtctat gatctgattc ttctagatct catgttacct gaaaaaaacg
     7981 gcttccaagt gttgaaagag ctacgtgaaa aaggtattac aacccctgta ttaataatga
     8041 ccgctaaaga aagcattgat gataagggac aaggtttcga cttaggtgct gatgattatc
     8101 taacaaaacc tttttaccta gaagaactaa aaatgcgaat tcaggctctt ttaaagcgtt
     8161 ctggaaaatt taatgataac agcctaattt atggtgatat tcgcgtagac atgtctacta
     8221 actcaacctt tgttaatcaa acagaagttg agctcttagg taaagagttt gatcttttag
     8281 tctatttcct tcaaaatcaa aatgttatct taccgaaatc tcaaatcttt gatcgtattt
     8341 ggggatttga tagtgataca acgatttcag ttgtagaagt ctatgtctct aaggttcgga
     8401 aaaaattaaa aggaacactt ttttcagaaa accttcaaac tttgcgtagt gtaggatata
     8461 ttttaaagca tgtcgaaacc gattaagata agtaaattca aaaaaaatgt ctcagatagt
     8521 cattttatcc attttttcac agtttttagt ggtatttttc tggttatgac tgttatcatc
     8581 ctccaagtca tgcgctatgg tgtttattct tctgttgata gtagtttaaa gtatatcagt
     8641 acccatccta aaaattacat caatatggtt atgtcacgta ctgctgccta cctagataac
     8701 tccaatatcg caagtgtcaa gttaaaaccc ggcggtcaga cagtagctaa tactgatatt
     8761 attcttttta catcagaaga agaagtcatt aactattttg acgcattttc aaactatcag
     8821 tttttaaaac ctaataaaaa aaatctaggt ggcattagtg aattaacttt aaccaacatc
     8881 tttggtcaag atgagacata tcatgctgtc accgtaaaag ttaataaccc tgcttatcct
     8941 aatgtgactt acatgacagc tattgttaat attgatcaat tagtaaatgc taaagaacgc
     9001 tatgaaaaaa ttattatatt tgtcatgact actttttgga ttatttcaat tggagcaagt
     9061 atctacttag ctaaatgggc acaaaaaccg attatagaaa actacgaaag acagaaggct
     9121 tttgtcgaaa atgctagcca cgagctgcgt actcccctag ctgtcttaca aaatcgccta
     9181 gaaacgctat tcagaaaacc gaatgccacc attttagaaa atagtgaaaa tatcgcttca
     9241 agtctagatg aagtacgaaa tatgcgtatt ttaaccacta atcttttaaa cttagcaaga
     9301 cgtgatgatg gaatcaaacc agaattagct gttattaagc ctacactttt tgatagtatc
     9361 tttgagaatt atgatctaat cgcccaagag aatggtaaaa attttactgg acataatatg
     9421 atccaagata gtttcaaaac agataagaca ctgctaaaac aactcatgac tattttattt
     9481 gataatgcta taaaatatac tgacaatgat ggtagcattg actttaccat atctgaaact
     9541 gataagtatc tctttttaga aatcgctgat aatggaccag gaatttcaga ggaggataaa
     9601 gtcagaattt ttgatcgttt ttaccgtgtt gacaaagcaa gaacacgaca acaaggaggg
     9661 tttggcctcg gcctttcttt agctcaacaa atcgttaact cactgcgagg taatattaca
     9721 gtaattgata ataaacctag aggcagtatt tttaagataa aactaccaaa agtaaaaggt
     9781 taggccatgt tagtcctaac cttttaaatt ttaaagtggt cttgcatact tgatatagca
     9841 agctgatagg aaatttggta taataaaagc tatggaaatt gaaaaaacaa atcgtatgaa
     9901 tgcccttttt gagttttatg cggcattatt aacagataaa caaatgaatt atatcgagtt
     9961 atactacgcc gatgactaca gccttgctga aatagctgaa gaatccggtg ttagccgaca
    10021 agctgtctat gataatatca aacgaactga aaaaattctt gaggcgtacg aaatgaaact
    10081 acatatgtat tcagattata ttgtccgtag tcagattttt gatgacattc tagaaaaata
    10141 taccgatgat gcctttttac aagaaaaaat ttccattcta agtagcattg ataatagaga
    10201 ttaaatgagg agaaataatt tatggctttc gaaagtttaa cagagcgcct gcaaggagtt
    10261 tttaaaaata ttcgtggtaa gaaaaagtta tctgaaaaag atgtgcaaga agtcaccaag
    10321 gagattcgtc tagctctttt agaagctgac gttgctcttc cagttgttaa aacctttatt
    10381 aaacatgttc gtgaacgtgc tgttggacat gaaatcattg atacactaga tcctacgcaa
    10441 caaattgtta agattgtcaa tgaagagcta acagatttat taggggctga gacctctgaa
    10501 attgaaaaat cccctaaaat tccaaccatc attatgatgg ttggtctaca aggtgctggt
    10561 aaaacaactt ttgccggtaa gttagcaaac aaactaatca aagaagacaa tgctcgtcct
    10621 atgatgatag cagctgatat ttatcgacca gctgctattg atcagttaaa aacccttggt
    10681 agccagatta atgttcctgt ctttgatatg ggaactaacc attcagcagt tgagattgtc
    10741 actaaaggtt tagaacaggc tcgtgaaaat cgtaatgact atgttcttat tgatacagcc
    10801 ggacgcctac aaattgatgc aacccttatg caggaattgc atgatgtcaa agccatagct
    10861 caacctaatg aaatactcct tgtcgttgac agcatgattg gacaggaagc tgcaaatgtc
    10921 gctgaagaat ttaaccgtca gctcagcatt agtggtgttg ttctgacaaa gattgacggt
    10981 gatacacgcg gtggtgctgc cctttcagta cgcgaaatta ctggaaaacc aattaaattt
    11041 acagggacag gggaaaaaat cactgatatt gaaactttcc atcccgatcg tatggcaagc
    11101 cgtatattgg ggatgggaga cctgctaaca cttattgagc gcgctagtca agaatacgac
    11161 gaaaaacgtt caatggaatt agcggagaaa atgcgtgaaa acacctttga cttcaacgat
    11221 tttattgatc aattagatca agttcaaaat atggggccaa tggaagatct ccttaaaatg
    11281 cttccaggaa tggctaacaa tccagcaatg aaaaacttca aagtggatga aaacgaaatt
    11341 gctcgcaaaa gagccattgt gtcatcaatg actccagaag agagagaaaa tccaaatctc
    11401 ctcaatccaa gtcgtagacg tcgtattgct gctggatcag gtaatacttt cgttgatgtt
    11461 aataaattca ttaaagattt taaccaggca aaacaaatga tgcaaggcgt gatgtccggt
    11521 gatatgaata aaatgatgaa aaaaatggga attgatccta ataacctccc taaagatatg
    11581 cctggaatgg acggaatgga tatgtctaat ttagaaggta tgatggggca aaatggcatg
    11641 cctgacttat ctagtttagg tggcgatatg gacttcagcc aaatgtttgg tggtggctta
    11701 aaaggaaaag taggagcctt tgcagctaaa caatcaatga aacgtatggc taataaaatg
    11761 aagaaagcta agaagaaacg caaataaaaa tgtctattta aatagacatt tttatttttg
    11821 tctgctataa tagacaaaaa ggtggtgata tttatgtatt tagcattaat cggtgatatc
    11881 attaattcaa aacagatact tgaacgtgaa actttccaac agtcttttca gcaactaatg
    11941 accgaactat ctgatgtata tggtgaagag ctgatttctc cattcactat tacagctggt
    12001 gatgaatttc aagctttatt gaaacaatca aaaaaggtat ttcaaattat tgaccatatt
    12061 caactagctc taaaacctgt taatgtaagg ttcggcctcg gtacaggaaa cattataaca
    12121 tccatcaatt caaatgaaag tatcggtgct gatggtcctg cctactggca tgctcgctca
    12181 gctattaatc atatacatga taaaaatgat tatggaacag ttcaagtagc tatttgcctt
    12241 gatgatgaag accaaaacct tgaattaaca ctaaatagtc tcatttcagc tggtgatttt
    12301 atcaagtcaa aatggactac aaaccatttt caaatgcttg agcacttaat acttcaagat
    12361 aattatcaag aacaatttca acatcaaaag ttagcccaac tggaaaatat tgaacctagt
    12421 gcgctgacta aacgccttaa agcaagcggt ctgaagattt acttaagaac gagaacacag
    12481 gcagccgatc tattagttaa aagttgcact caaactaaag ggggaagcta tgatttctga
    12541 ttttttaagg gataatccaa tcctaacttt attattttgc gcacattttt tagctgactt
    12601 tcagtggcaa agtcaatcct tagcagatag taaatcacat agttggaggg gactctggag
    12661 acatctcttg attgttttcc tccccctagc agctttaatg atattgatac cagaaactac
    12721 tttgttaaat ttaagcatat ggggaagtca tattgttatt gatagtataa agaaattgag
    12781 ttacccttgg gtagaggaag ggcatttcca aaaagcagct tttattatag accaactagc
    12841 ccactatact tgcattatcg ttttttacca tgcgcttcct acatatttac cgcctaatca
    12901 ttggcttttg cctatcaagc actttattgt catagctctg gtttttatca ttatcaccaa
    12961 acccattaat attgtgttta aaattttctt taataaattt caagctaaag aattatcatc
    13021 attattaaca caagaaaaaa caaaaataat gaaagaaaaa tctgaagatc atgaagaaac
    13081 aattgaagga gcaggagcta tgattggtaa tttagagcgt ctaattatgg ctatattact
    13141 aatctcagga cagtatgctg ctataggtct tgtttttact gcaaaatcca ttgcacgtta
    13201 cgataaaatt tctaaaagtc aggtttttgc cgaatattat ttaattggat cattgttctc
    13261 aattataagc gttttgataa ctcattggtt acttttggta tagaaaagcg ctagaaatat
    13321 cctaacgctt ttcttttatt ctgtcttata agcataagta aaatcaacat tcaaaccagt
    13381 tgaatttctt accaacccct ttaatttaga attttggagt ccttttttcc ctaggtaata
    13441 aactgggtta taatatgatt gatcaaataa agctttttca gcagttttgt aatcattagc
    13501 ggcatctcct ggtttcaagg cgtcagtagt aattgccttt tgataggcat tgtcatactc
    13561 tttagaagaa aatttaccat aattatacgc agaattagtc gtgaataacc catagaaagt
    13621 tgatccttct ggataatctc ctccccatga gaagagcacg acatcaaaat tttcattttt
    13681 tgcatcttca aggcgttgct tgaaagtaac aaatttttct tcaacagtta gaccaggcaa
    13741 agcactctcc catgtagatt tcacatagtc aacagcattt ttagctgctg gagagtccga
    13801 atcagctgtg attgttaact tgagagatga ctgaccaacc tcagcaagac cttctttaaa
    13861 aagtttttga gctttacttg tattatacgt ataacctggg gcagtatact tggaaagatc
    13921 ctcaccatta ggtaatttgg ctaatttttt agggaccaga gattcagcag gagttgaacc
    13981 tgtatcaact gccgctttaa caacaccttt acgatcagtc gctaaattca aagcttgcct
    14041 aattttctga tttgtcaatg ctttaaccga accagtttgg ttataaatag cagaagttcc
    14101 tgatatttca gcaaaatcaa tctgaccttg cttgtacatt tgaactgcag tatctggttt
    14161 tttgatagtt tgaactatga cactatttgt tttaacatgt ttagaatccc aataatattt
    14221 atttttgact aatttaaatt taccatttga accattccaa tccttaacaa ggtaaggacc
    14281 tgagtaaatt tgattttttg atgttgtacc ataatcttta ccaacttttt caacatacga
    14341 ttgtttttga ggcataaaat tagagaaggc taaataatat ttaaattgag ggcatggact
    14401 tgtcaattta aaggtcactt gattgccttt agctgttaca ccaagtttat ttaaatcttt
    14461 aatatctcca gaattaatct tatcagcatt taaaaggtgc gattctgtcg ccaagtaagc
    14521 atattcagat gcagttttag gatcaactat tctccgccat gtgtaaacaa aatcttcagc
    14581 gcttagtttg cttccatctg accattttaa attatcacga agtgtagcag tataagtaag
    14641 accatcagaa gaaacggata cttttttagc taaatcgggt tttggtttgc cctctttatc
    14701 aattctaagt aaattgctgc ctgaattacc tatagctaag tttgaataag catctgtatt
    14761 ctttgaaatg tccaatgtac tgatttcagt aggaatatac caattaataa ctttattatc
    14821 tccagagttc tttgactcat taccacaggc aaccagggta actgtggtgg ctgttagagt
    14881 cgccaaagat atacccaaaa acttaagttt ttttctatta aaactagaca taataccagt
    14941 cctcctaaac ctttttttat aagacatagt agatctaata ttgagaaaac tagtatattt
    15001 taaagtagaa tttaactttg gttattaact tgcaaatata attaaaaata attgagtaca
    15061 ctttattacc ttttcagata ccgataaaat caatgaaatt atgaatcagt tttc
//
LOCUS       DATROQ010000043        14975 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.043, whole genome shotgun sequence.
ACCESSION   DATROQ010000043 DATROQ010000000
VERSION     DATROQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14975
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.043"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            212..1354
                     /locus_tag="VBC94_001201"
     CDS             212..1354
                     /locus_tag="VBC94_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6979476.1"
                     /translation="MWSEKHKSGKVNFIERYKDPYTSRWKRTSVLMEKDTPRIRKEAQ
                     RILDAKIADIVRKLQTSDMLFTDLLDQWWGFYQQEIKRSSIASLRGNIRDIRESFGID
                     IKVVNIDPKYVQTYLDNLDCSRNKKERNKSMLNLIFDYAVSLDIIKDNPARRAKLPRI
                     KKTLEDWKKIEEKYLEEEEIKRLLKELFRRPSTYRLGLLSEFMSLNGCRIGEAISIEP
                     DNIDFKNKTLQLHGTYDRTNGYMNGEKTSPKTLASYRETIMTKREMEILQEMEFINEL
                     EKNTNKRYRDMGYIFTTKNGIPVQINSFNLALKKANKRLKHPINKNITSHIFRHTLVS
                     RLAENNVSLKAIMDRVGHADAKTTVQIYTHITKKMKSNVADIMENY"
     gene            complement(1443..1718)
                     /locus_tag="VBC94_001202"
     CDS             complement(1443..1718)
                     /locus_tag="VBC94_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEO6979477.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFAAVADYLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            complement(1821..2423)
                     /locus_tag="VBC94_001203"
     CDS             complement(1821..2423)
                     /locus_tag="VBC94_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEO6979478.1"
                     /translation="MKQEKTGRNLNFWKWAFLLLLAINLSFTAVIASRLIQVREPNTG
                     KISTGVQDKVKVGTFTTNKSQLNKTIALYLKQYQTKKMNYKIYAASSSILFEGSYQLL
                     GYEVPLYIYFEPYRLTNGAVQLKVTSFSVGTLPLPEKDVLQYIKSSYKLPNFVDIKPK
                     KSVININLQDLKNKEGIYLKATAIDLVNDNFSFDIFKKKP"
     gene            complement(2398..3237)
                     /locus_tag="VBC94_001204"
     CDS             complement(2398..3237)
                     /locus_tag="VBC94_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO6979479.1"
                     /translation="MNKKKILTGLSFFLVSLLLSFGIFSLIIPKSNPKLTKKDFLTKK
                     VIPLNYVALGDSLTEGVGDTTSQGGFVPLLSESLHNRYSYQVTSVNYGVSGNTSQQIL
                     KRMTTDPQIEKDLEKADLLTLTVGGNDVLAVIRKELSHLSLNSFEKPAEAYKERLKEI
                     LAKARQDNPKLPIYVLGIYNPFYLNFPQLTKMQTVIDNWNKATKEVVDASENVYFVPI
                     NDRLYKGINGKEGITESSNSQASITNDALFTGDHFHPNNIGYQIMSNAVMEKINETRK
                     NWP"
     gene            complement(3230..4066)
                     /locus_tag="VBC94_001205"
     CDS             complement(3230..4066)
                     /locus_tag="VBC94_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6979480.1"
                     /translation="MSKIKIVTDSSITIEPELIKELDITVVPLSVMIDGTLYSDNDLK
                     AQGEFLNLMRGSKELPKTSQPPVGVFAEIYEKLMNEGAEHIIAIHLTHTLSGTIEASR
                     QGANIAGADVTVIDSTFTDQCQKFQVVEAAKLAKEGADLDTILARVEEVRQKSELFIG
                     VSTLENLVKGGRIGRVTGLLSSLLNIKVIMELTNHELVPIVKGRGLKTFSKWLDNFVE
                     SAQTRKIAEIGISYCGKADMANNFKEKLAVLGAPISVLETGSIIQTHTGEDAFAVMVR
                     YE"
     gene            complement(4179..5837)
                     /gene="recN"
                     /locus_tag="VBC94_001206"
     CDS             complement(4179..5837)
                     /gene="recN"
                     /locus_tag="VBC94_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEO6979481.1"
                     /translation="MLLEISIKNFAIIEEISLNFETGMTVLTGETGAGKSIIIDAMNM
                     MLGARASVEVIRHGANKAEIEGFFSIEKNQSLVQLLEENGIELADELIIRREIFQNGR
                     SVSRINGQMVNLSTLKAVGHYLVDIHGQHDQEELMKPNMHILMLDEFGNTEFNVIKER
                     YQSLFDAYRQLRKRVLDKQKNEQENKSRIEMLEFQIAEIESVALKSDEDQTLLKQRDK
                     LMNHKNIADTLTNAYLMLDNEEFSSLSNVRSAMNDLMALEEFDREYKDLSTNLSEAYY
                     VIEEVTKRLGDVIDDLDFDAGLLQEIENRLDVINTITRKYGGDVNDVLDYFDNITKEY
                     SLLTGSEESSDALEKELKILEHDLIESANQLSLERHKLAKQLENEIKQELTELYMEKA
                     DFQVQFTKGKFNKEGNEIVEFYISTNPGEGFKPLVKVASGGELSRLMLAIKSAFSRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIHKIGSHGQVLAISHLAQVIAIADYQYFIEKIS
                     SDSSTVSTVRLLSYEERVEEIAKMLAGNNVTDTARTQAKELLGS"
     gene            complement(5849..6322)
                     /locus_tag="VBC94_001207"
     CDS             complement(5849..6322)
                     /locus_tag="VBC94_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEO6979482.1"
                     /translation="MKKSERLNLIKQIVLNHAVETQHELLRRLEAYGVTLTQATISRD
                     MNEIGIIKVPSAKGRYIYGLSNENDPIFTTAVAKPIKTSILSISDKLLGLEQFININV
                     IPGNSQLIKTFIMSHCQEHIFSLTADDNSLLLIAKSEADADHIRQSMIAMLEKKD"
     gene            complement(6309..7136)
                     /locus_tag="VBC94_001208"
     CDS             complement(6309..7136)
                     /locus_tag="VBC94_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEO6979483.1"
                     /translation="MAKERVDVLAYKQGLFDTREQAKRGVMAGLVINVINGERYDKPG
                     EKVADDTELKLKGEKLKYVSRGGLKLEKALQVFEISVADKLTIDIGASTGGFTDVMLQ
                     SGARLVYAVDVGTNQLVWKLRQDHRVRSMEQYNFRYAQKEDFKEGLPEFASIDVSFIS
                     LNLILPALKEILVDGGQVVALIKPQFEAGREQIGKNGIVKDKLVHEKVLTTVTNFTKD
                     YGYTVKHLDFSPVQGGHGNIEFLMHLQKCQDPQNLVLDQIQDVIEKAHKEFKKNEEE"
     gene            complement(7129..8001)
                     /locus_tag="VBC94_001209"
     CDS             complement(7129..8001)
                     /locus_tag="VBC94_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO6979484.1"
                     /translation="MVTIEKIDEAIHRHYKQTHSVVSPDLIKAILYSVDGGGKRIRPR
                     ILLEILEGFGVELIDGHYDVAAALEMIHTGSLIHDDLPAMDNDDFRRGRLTNHKKFDE
                     ATAVLAGDSLFLDPFDLVVKAGFKADVTVRLIELLSMSAGSFGMVGGQMLDMKGENKV
                     LSIDDLSLIHINKTGRLLAYPFVAAGILAEKSEEVKGKLHQAGLLIGHAFQVRDDILD
                     VTASFEELGKTPNKDIVAEKTTYPNLLGLDKSQEILDDTLKKAQAIFQNLEKKANFNA
                     RKIIDIIEGLRLNG"
     gene            complement(8001..8216)
                     /locus_tag="VBC94_001210"
     CDS             complement(8001..8216)
                     /locus_tag="VBC94_001210"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEO6979485.1"
                     /translation="MSDKKTFEENLQELETIVSRLETGDVALEDAIAEFQKGMLISKE
                     LQKTLKEAEETLVKVMQADGTEVEMDT"
     gene            complement(8194..9534)
                     /locus_tag="VBC94_001211"
     CDS             complement(8194..9534)
                     /locus_tag="VBC94_001211"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEO6979486.1"
                     /translation="MSDYLSVSTLTKYLKLKFDKDPYLERVYLTGQVSNFRRRPNHQY
                     FSLKDDKSVIQATMWSGQFKKLGFELEEGMKVNVVGCVQLYEPSGSYSIIVEKAEPDG
                     IGALAIQFEQLKKKLSQAGYFDDRHKQLLPQFVRKIGVVTSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGEGAAQEIAQTIALANEKKDLDLLIVGRGGGSIEDLWAFNEECVV
                     EAIFESRLPVISSVGHETDTTLADFVADRRAATPTAAAELATTVTKIDILSWITEREN
                     RMYQSSLRLIRTKEERLQKSKQSVIFRQPERLYDGFLQKLDNLNQQLTYSMRDKLQTV
                     RQKQGLLHQKLQGIDLKQRLHTYQERVVQSRRLLSSTMTSQYDSKLARFEKAQDALIS
                     LDSSRIVARGYAIIEKNHTLVSTTNGINEGDHLQVKMQDGLLEVEVKDVRQENI"
     gene            complement(9660..10496)
                     /locus_tag="VBC94_001212"
     CDS             complement(9660..10496)
                     /locus_tag="VBC94_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEO6979487.1"
                     /translation="MIVGEQEARALIKPRPKSSHKGDYGSVLLIGGFYPYGGAIIMAA
                     LACVKTGAGLVTVATQSCNIPSLHSQLPEVMAFDSDDYKWLEKSIVQSDVIVIGPGLG
                     VSESSRKILNRTMEKIQSHQSVIIDGSALTLLSEGAFPQTKAKNLVLTPHQKEWERLS
                     GIAVSQQTKENTQTALKSFPKGTILVAKSSHTRIFQDLDEKEIIVGGPYQATGGMGDT
                     LCGMIAGMLAQFKEASPLDKVSVGVYLHSAIAQELSKEAYVVLPTTISDEIPKEMARL
                     SK"
     gene            complement(10493..11347)
                     /locus_tag="VBC94_001213"
     CDS             complement(10493..11347)
                     /locus_tag="VBC94_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEO6979488.1"
                     /translation="MTELIDGKALSQKMQAELGRKVERLKEQHGIIPGLAVILVGDNP
                     ASQVYVRNKERSALEAGFKSETLRLSESISQEELIDIIHQYNEDKSIHGILVQLPLPQ
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPMMVPCTPAGIMEMFREYHVDLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRNLSEVTKEADILIVAIGQGHFVTKD
                     FVKEGAVVIDVGMNRDENGKLIGDVVFEQVAEVASMITPVPGGVGPMTITMLLEQTYQ
                     AALRSVSL"
     gene            complement(11486..13180)
                     /locus_tag="VBC94_001214"
     CDS             complement(11486..13180)
                     /locus_tag="VBC94_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO6979489.1"
                     /translation="MNYKEIYQEWLENDSLGKDIKSDLEAIKGDESEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAIARGIAVSYDVRYQSKEFA
                     ELTCSIMAANGIKSYIYKGIRPTPMCSYAIRALGCVSGVMVTASHNPQAYNGYKAYWK
                     EGSQILDDIADQIANHMDAITDYQQIKQIPFEEALASGLASYIDESIEEAYKKEVLGL
                     TINDTNIDKSVRVVYTPLNGVGNLPVREVLRRRGFENIYVVPEQEMPDPDFTTVGYPN
                     PEVPKAFAYSESLGKSVDADVLLATDPDCDRVALEVKDSKGEYIFLNGNKIGALLSYY
                     IFSQRCALGNLPHHPVLVKSIVTGDLSKVIADKYNIETVETLTGFKNICGKANEYDIS
                     KDKTYLFGYEESIGFCYGTFVRDKDAVSASMMVVEMTAYYKERGQTLLDVLQTIYDEF
                     GYYNERQFSLELEGAEGQERISRIMEDFRQNPILQVGEMTLENSIDFKDGYKDFPKQN
                     CLKYYFNEGSWYALRPSGTEPKIKCYLYTIGCTEADSLSKLNAIESACRAKMNSTK"
     gene            complement(13467..14201)
                     /locus_tag="VBC94_001215"
     CDS             complement(13467..14201)
                     /locus_tag="VBC94_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6979490.1"
                     /translation="MVEPIISIQGLHKSFGKNEVLKGIDLDIHQGEVVVIIGPSGSGK
                     STFLRTMNLLEVPTKGTVTFEGIDITDKKNDIFKMREKMGMVFQQFNLFPNMTVLENI
                     TLSPIKTKGLSKLDAQTKAYELLEKVGLKEKANAYPASLSGGQQQRIAIARGLAMNPD
                     VLLFDEPTSALDPEMVGEVLTVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDAGII
                     VEQGTPKKVFEQTKEIRTRDFLSKVL"
     gene            complement(14194..14886)
                     /locus_tag="VBC94_001216"
     CDS             complement(14194..14886)
                     /locus_tag="VBC94_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6979491.1"
                     /translation="MNFSFLPQYWSYFNYGVMVTIMISTCVVFFGTIIGVLIALVKRT
                     NLHFLTILANFYVWVFRGTPMVVQIMIAFAWMHFNNLPTISFGVLDLDFTRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPSGQIEAAYSLGIRPKNTLRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPVAPLLFAAFYYLMLTTILSALLKPM
                     EKYLGKGVKIDG"
BASE COUNT         4793 a         3015 c         2261 g         4906 t
ORIGIN      
        1 taacaaaacc aagtaaagga atagagtata ttttctactc taatggatta acagtcccag
       61 aaaatacaat aaatataaaa aaagcaaatt aaaaaaagac ctacacagcg ccggcaagca
      121 aacgtgtagg tcaagttggt aatagtaaaa acctgcttta agtaggctct ttactgtacc
      181 cattttaaca aaaaaaatga ggtaaaaaac aatgtggtca gaaaaacata aaagcggaaa
      241 agtgaatttt atcgaaagat ataaagatcc gtatacaagc agatggaaac gtacatcagt
      301 actgatggaa aaagatactc ctcgaattag aaaagaagct caaagaatat tagatgctaa
      361 aatagctgac atcgtaagaa agttacaaac ctccgacatg ctatttacag accttcttga
      421 ccaatggtgg ggattttacc aacaagagat aaaacgctcc tctatagcct ctctgagagg
      481 caatataaga gatattagag aaagctttgg aattgatatt aaggttgtca atatcgaccc
      541 taaatatgtc caaacctact tggataactt agattgctcc aggaacaaaa aagagcgaaa
      601 caagtccatg cttaatctta tctttgatta tgctgttagt ctagatatta tcaaagataa
      661 tcctgcaagg cgtgctaaac ttccgcggat aaagaagact ttggaagatt ggaaaaagat
      721 tgaagaaaaa tatcttgaag aagaagaaat caaacgacta ttaaaagaat tatttagaag
      781 acctagcact taccgtctgg gattactatc agaatttatg agtcttaacg gatgccgtat
      841 cggtgaggct attagcatag agccggataa tattgatttt aaaaacaaaa ctttacagtt
      901 gcatggtact tatgatcgta ccaatggtta catgaatgga gaaaagacct ctccaaaaac
      961 gcttgcgtcc tatcgcgaaa ccattatgac taagcgagaa atggaaattt tacaagagat
     1021 ggaatttatt aatgagctcg aaaaaaatac aaataaacga tatagagaca tgggatatat
     1081 ctttacaacc aagaatggta ttcctgtaca aataaattcc tttaatttag cattgaaaaa
     1141 agcaaacaaa agacttaagc atccaatcaa taaaaatatc actagtcaca tcttccgcca
     1201 cacattagtc agtcgtcttg ctgaaaataa cgtatcttta aaggcaatca tggatcgtgt
     1261 aggacacgct gacgcaaaaa ctacagtaca aatttataca cacataacca agaaaatgaa
     1321 atcaaatgta gctgatatca tggaaaacta ctaatttcat gccccaaata tgccccaaaa
     1381 ataaaaaaaa gaaccttgtc gctatcaata aacattgata tgacaaggtt tttctagacg
     1441 aattatttaa cagcgtcttt aagagcttta ccagctttaa atgctggaac ttttgatgct
     1501 gcaatttcga tttctgcacc agtttgtggg ttacgacctt tacgagctgc acgttcacga
     1561 acttcaaagt taccaaaacc gattaattgt actttttcac cttcagcaag gtaatctgct
     1621 acagctgcaa aaactgcatc tactgctgct gctgaatctt ttttagttaa ttctgtagct
     1681 tctgctactt ttgcaattaa atcttgtttg ttagccatgt taacaaatcc tccaaataaa
     1741 ttctaaactt atacaacata agttttaaca gattatatga tatctaaaaa aaggcttaag
     1801 gtcaagtatt taacgtcatt ttagggcttt ttcttaaaaa tatcgaaact aaaattatcg
     1861 tttactaaat caattgcagt cgctttcaaa taaatacctt ctttattttt taaatcttgc
     1921 aaattaatat taataaccga ttttttaggt tttatatcta caaagtttgg caatttataa
     1981 gatgatttta tatattgtaa cacatctttt tctggaagcg gaagagttcc tactgaaaat
     2041 gaggtcactt taagttgaac agcgccattt gttaaacgat aaggttcaaa atagatgtat
     2101 aaaggcactt cataacctaa taattgataa gagccttcaa atagtataga agatgaagca
     2161 gcataaatct tataattcat cttcttagtt tgatattgtt ttaaataaag tgcaattgtc
     2221 ttattcagtt gcgacttatt ggtcgtaaaa gtacctactt ttactttatc ttgtacccca
     2281 gtcgaaattt ttcctgtatt aggctcacgt acttgaatta aacgacttgc aatcactgct
     2341 gtgaaggaaa ggttaattgc caacaataag agaaatgccc atttccaaaa atttaaatta
     2401 cggccagttt tttcttgttt catttatttt ctccataacg gcgttagaca tgatttgata
     2461 gccaatatta ttgggatgaa aatggtctcc agtaaagaga gcatcattag tgatacttgc
     2521 ctgactattt gatgactctg taataccctc tttaccattt attcccttat aaaggcggtc
     2581 attaattggg acaaaataaa cattttctga agcatcaact acttcttttg tagctttatt
     2641 ccaattatca ataacggttt gcattttagt taattgtgga aagtttaggt aaaaaggatt
     2701 ataaatgcct aaaacataaa taggcaattt aggattatct tgtcttgctt ttgcaagaat
     2761 ttctttcaaa cgttccttat atgcttctgc tggtttctca aaggaattta gtgataaatg
     2821 actgagctct ttacgaataa cagccaagac atcattacca ccaacagtta gcgtcaataa
     2881 atcagctttc tctaaatctt tttcgatttg aggatctgtc gtcatacgtt ttaaaatttg
     2941 ttgactagta ttcccagaca caccataatt aacagaagtc acttggtaag agtatcgatt
     3001 atggagtgat tctgatagca gtgggacaaa accaccttga gaggttgtat cccccacacc
     3061 ttcggtcaga gaatctccaa gagcaacata gttaagtggg ataactttct ttgttaggaa
     3121 gtcttttttt gttaatttag gatttgattt tggaatgatc aaggaaaaaa tcccaaaaga
     3181 taaaagaaga cttaccagaa aaaaagaaag tccggtaagt atttttttct tattcataac
     3241 gaaccataac agcaaatgca tcctcaccag tatgagtttg gatgattgag ccagtttcta
     3301 atacggatat aggagcacct agaacagcta gtttttcttt aaagttatta gccatatcag
     3361 ctttaccaca gtaggagatg ccaatttcag caatttttcg tgtttgggca ctttcaacaa
     3421 aattatctaa ccatttgctg aaggtcttta gaccgcgacc tttaacaatc ggtactaatt
     3481 catggtttgt tagctccata attactttta tgttaagcaa tgaacttagg agacctgtta
     3541 cacgtccaat acgaccacct tttactaaat tttcaagggt cgatacgcca ataaataatt
     3601 ctgacttctg gcgtacttct tccacacgag ccaaaattgt atctaaatca gctccctctt
     3661 tagctaattt cgcggcttct acaacctgga atttttgaca ctggtctgta aaagtagaat
     3721 caataactgt aacatctgca ccagcaatat tagctccctg gcgtgatgct tcaatagttc
     3781 ctgatagtgt atgcgttaaa tgaattgcaa tgatatgttc cgcaccttca ttcattaatt
     3841 tttcataaat ttctgcaaaa acacctacag gtggttgact tgtttttggc aattccttac
     3901 ttccacgcat taggttcaag aattcccctt gtgcttttaa gtcattgtct gaatacaagg
     3961 taccatctat cataactgac aagggaacaa ctgtaatgtc aagctcttta ataagttctg
     4021 gttcaatggt aatggaagaa tccgttacaa ttttgatttt actcatatcc tatcctattc
     4081 tatactttaa tatttcaata cccttattat aacaaaattt aagtgtatca acattaattt
     4141 ctcctaacaa tgttagcttt ttaaagacgt taccaaagct aactaccaag taattcttta
     4201 gcttgagtac gtgcagtatc agttacattg tttcctgcca acattttagc gatttcttct
     4261 acacgttctt catagcttaa aagccttacc gttgaaactg ttgaagagtc actggaaatt
     4321 ttttcaataa aatattggta atccgctatt gcaataacct gagctaaatg agaaattgct
     4381 aaaacttgtc catgactacc aatcttgtga attttttgag caatagcctg ggccacgcga
     4441 ccagaaactc ctgtatccac ctcatcaaat acaatggaag ttttatcttc cttacgtgaa
     4501 aaagcagatt taatagctaa catcaatcga gataactctc ctcctgaagc cacttttacc
     4561 aatggcttaa agccctctcc gggattcgtt gaaatataaa attcaacgat ttcgttccct
     4621 tctttattaa attttccttt tgtaaattga acttggaaat cagctttctc catataaagc
     4681 tctgttaatt cttgtttgat ttcattttcc aattgtttag ctagcttatg acgctctaaa
     4741 cttaattgat ttgctgattc aatcaaatcg tgttctaaaa tctttaattc tttttctaaa
     4801 gcatctgagg actcttcact acctgtcaat agactatatt ctttagtaat gttatcaaaa
     4861 tagtctaaaa catcattcac atcaccgcca tattttcgcg ttattgtgtt aataacatct
     4921 agtctatttt caatttcttg tagtaaacca gcgtcaaaat ctaaatcatc gataacgtca
     4981 cctaaacgtt tagtaacttc ttcaataacg tagtaagctt ctgaaagatt ggtggaaaga
     5041 tctttatatt ctcgatcaaa ttcttctaaa gccataaggt cattcattgc agaacgaaca
     5101 ttcgataaac ttgaaaactc ttcgttatct aacataagat atgcatttgt caaagtatct
     5161 gcaatattct tatgattcat taatttatca cgttgcttga gtagcgtttg gtcttcatct
     5221 gatttaaggg ctacagactc aatttctgct atttgaaatt ctagcatttc aatacgtgat
     5281 ttattctctt gttcattttt ttgcttatcc aatacacgtt tacgaagctg acgataagca
     5341 tcaaaaagac tttgataacg ctcttttatg acattaaatt ctgtattacc aaattcatct
     5401 aacatcagaa tgtgcatgtt aggtttcatt agttcctctt ggtcatgctg accatgaatg
     5461 tctaccaaat aatgccccac agcttttagt gttgacaaat tcaccatttg cccattaatt
     5521 cgactaacgc tgcgcccatt ttgaaagatt tctcggcgaa taattaattc atctgctaat
     5581 tcaatgccat tttcttccaa taattggact aatgattgat ttttttcaat agagaaaaat
     5641 ccttcaattt ctgctttgtt agcaccatgg cgaatcactt caacactagc acgggctcct
     5701 aacatcatat tcatagcatc aataatgata gatttccctg caccagtctc accagttaaa
     5761 acagtcattc ctgtttcaaa attaagtgaa atttcttcga taatagcgaa attcttaata
     5821 gaaatttcta aaagcataag gtcctctact aatctttttt ctccaacatt gcaatcattg
     5881 attgacgaat gtgatcagca tctgcttctg attttgcaat caaaaggaga ctattatcgt
     5941 cagctgtcaa actaaaaata tgttcttgac aatgtgacat tatgaaggtt ttaattaatt
     6001 gactgttacc tggtatgaca ttaatattga taaattgctc taaacctagt agcttatctg
     6061 atattgataa aatacttgtt ttaataggct ttgccacagc agttgtaaag atagggtcgt
     6121 tttcatttga caaaccgtaa atatagcgac cttttgctga tggcactttt ataatgccaa
     6181 tttcattcat atcacgtgaa atagttgctt gagttagagt taccccataa gcttccaatc
     6241 tgcgtaacaa ctcatgttgt gtttcaacag catggttaag aacaatttgc ttaattaaat
     6301 ttaaacgttc actcttcttc atttttctta aattccttat gtgctttttc tataacatct
     6361 tgtatttggt caagcacaag attttgtgga tcttgacact tttgcaaatg cattaaaaac
     6421 tcaatatttc catgtccacc ttgaacgggc gaaaaatcaa gatgtttaac cgtatatcca
     6481 taatctttcg tgaaattggt cactgttgtc aaaacctttt catgaaccaa cttgtctttg
     6541 acaataccat ttttaccaat ttgctcacga cctgcttcaa attgtggttt aattaatgcc
     6601 actacttgtc caccatccac taaaatttct tttagagctg gtaaaatcaa attaagagag
     6661 ataaatgaga catctatcga tgcaaattca ggcagtccct ccttgaaatc ttctttttgg
     6721 gcatacctaa aattatattg ttccatagaa cgaacacgat gatcctgacg taacttccaa
     6781 actaattgat ttgttcctac atctactgcg taaactaaac gcgctcctga ttgtagcata
     6841 acatcagtaa aaccacccgt agaggcgcca atatctatag ttagcttatc tgcaactgaa
     6901 atttcaaaaa cttgtaaagc tttttctaat ttcaatccac ctctactaac atattttagt
     6961 ttttcacctt ttagttttaa ttcagtatcg tctgcaacct tttcgcctgg tttatcataa
     7021 cgttctccat tgataacgtt aatcaccagt cctgccataa caccacgttt cgcttgctct
     7081 cgtgtatcaa aaagtccctg tttataggca agaacatcta ccctctcttt agccattcaa
     7141 ccgtaatccc tctattatat ctattatttt tctagcatta aagttagctt ttttctctag
     7201 attttgaaaa attgcctgag cttttttcaa agtatcgtca agtatttcct gtgacttatc
     7261 caaacccaat aaatttggat aagttgtctt ttctgctaca atgtctttat ttggtgtctt
     7321 acccaattct tcaaaactag cagtcacatc taaaatatca tcacgtactt gaaaagcatg
     7381 accgattaaa aggccagctt gatgcagttt tccttttact tcttccgact tctcagctaa
     7441 aatacctgct gcaacaaagg gataagctaa caggcgtccc gttttattaa tatgaatcaa
     7501 gcttaaatcg tcaatagata aaactttgtt ttctcccttc atatctaaca tttgtccacc
     7561 aaccatgcca aaactacccg cagacataga caataactct attaacctaa cagtaacatc
     7621 agctttaaaa ccagctttta caactaaatc aaatgggtct aaaaaaaggg aatctccagc
     7681 taaaaccgct gtagcttcat caaacttttt atggtttgtt aagcggccac gacgaaaatc
     7741 atcattatcc atcgccggta aatcatcatg aattaaagag ccagtgtgaa tcatctccaa
     7801 agcagcagcc acatcataat gaccatctat tagttcaact ccaaatccct ctaaaatttc
     7861 taaaaggatg cgtggacgaa tacgtttccc acctccatca actgagtaga ggatagcctt
     7921 aattaaatca ggagaaacga cactatgtgt ctgtttgtaa tgacgatgaa tagcttcatc
     7981 aattttttca atagtaacca tcaagtatcc atttctactt ccgttccgtc agcttgcatc
     8041 acttttacaa gcgtctcttc agcttctttt aaagtttttt gtaactcttt tgaaataagc
     8101 attccttttt gaaattcagc aatggcatct tctaaggcaa catcacctgt ttctaaacgt
     8161 gagacaattg tctctaattc ctgcaaattt tcttcaaatg ttttcttgtc tgacatcctt
     8221 cacctcaact tctaaaagac catcttgcat cttaacttgc aaatgatctc cttcatttat
     8281 tccattagta gtagatacta aagtatggtt tttttcgata atagcgtaac cacgcgctac
     8341 aattctagaa ctatccaatg atataagcgc atcttgagcc ttttcaaaac gtgctagctt
     8401 gctgtcatac tgactagtca ttgtacttga taatagtcgt ctactttgca caacacgctc
     8461 ttggtaagta tgaagccgct gttttaaatc tatgccttgc agtttttgat gaagcaaacc
     8521 ttgcttttgt cttactgttt gtagtttatc acgcatagaa tatgttaact gctgatttag
     8581 gttatctaat ttttgaagga aaccatcata tagacgctct ggttgcctaa aaataactga
     8641 ttgctttgat ttttgcaacc tctcctcttt tgtcctgata agacgaaggc tagactgata
     8701 cattctattt tcacgttcag taatccaaga caagatatca attttagtaa ccgttgtagc
     8761 aagctcagct gctgcagttg gagttgctgc tctgcgatcg gctacaaaat ctgctaaagt
     8821 tgtatcagtt tcatgtccga cactagaaat aacgggaaga cgagattcaa aaatagcttc
     8881 tacaacacat tcttcattaa atgcccataa gtcctcaata gaacctcccc cacggccaac
     8941 aattaataga tccaaatcct ttttctcgtt agctaaagca atagtttgtg ctatctcctg
     9001 agctgcgccc tctccttgaa cttttgtggg gaaaagtaat atttccactc ctgggaatcg
     9061 acgagatact gttgtaataa tatcccgaat aacagctcca cttggactag tcacaactcc
     9121 tatttttcta acaaattgag gaagtaattg cttatgacga tcatcaaagt aacctgcttg
     9181 agagagtttc ttctttagtt gttcaaattg gatagcaaga gctcctatac catccggttc
     9241 tgctttttca acaataatag agtaggagcc gcttggttca taaagttgaa cacagccaac
     9301 tacattaacc ttcatccctt cctctaattc aaaacccagt tttttgaatt gacctgacca
     9361 cattgtcgcc tgaatgactg atttatcatc ttttaaagag aagtattgat ggtttgggcg
     9421 acgacgaaaa ttagacactt gcccagttaa ataaaccctc tctaaataag ggtctttgtc
     9481 aaattttaat tttaaatact tagtcagtgt cgacacggat aaatagtctg acatagcacc
     9541 tcctcatgag agttttatta acttcattat accaaaaaaa cttaaaaaat acctatttgc
     9601 tctacaaata gctcgataaa gcaaaagaag tgtcatgcta acacctctct acttattttt
     9661 tatttagata gtctagccat ttcttttgga atctcatcac taatcgttgt cggtagaaca
     9721 acgtaggctt ctttagataa ttcttgagca attgccgaat gtagataaac tcccactgat
     9781 accttatcta gaggagaggc ttctttaaat tgcgctaaca tacctgcaat cataccacac
     9841 aaagtatccc ccatccctcc agtcgcctgg taaggacctc ctactataat ttctttttcg
     9901 tctaaatctt gaaaaatacg cgtatgcgaa ctcttagcga ctaagatcgt ccctttggga
     9961 aaagatttaa gagcggtttg ggtattttct tttgtctgtt gcgatacagc aatacctgac
    10021 aatcgctccc attctttttg atgaggtgtc aacactaaat tcttagcctt tgtttgcgga
    10081 aacgcacctt ctgataacag agtcaaggct gatccgtcaa tgatgacact ttgatgtgat
    10141 tgaatcttct ccatggtccg gttcaaaatt tttcgagatg attctgatac tcctaatcca
    10201 ggaccaatta caataacatc actttgaaca attgattttt ccaaccattt gtaatcatca
    10261 ctatcaaacg ccattacctc tggtagttga ctatgcaaag aggggatatt gcaactttgg
    10321 gttgctacag taactaatcc tgcaccagtt ttgacacagg ccaaagctgc cattataata
    10381 gcacctccat agggataaaa acctcctatc agaagaacac taccataatc acctttatgg
    10441 cttgatttag ggcgtggctt aattaacgcc ctcgcttctt gttcaccaac aatcatagac
    10501 tcacacttct aagagctgct tgataagttt gttccaaaag cattgtaatc gtcataggtc
    10561 ctacgccccc aggaacaggt gttatcatac tagcaacttc tgccacttgt tcaaatacaa
    10621 cgtctccaat caatttacca ttttcatcgc gattcatacc aacatcaatc acaacagcac
    10681 cttctttaac gaagtctttt gtaacaaagt gcccctgacc aatcgcaaca ataaggatat
    10741 cagcctcctt tgttacctca gaaaggtttc gagttcttga atgtgtcaaa gtaactgttg
    10801 cgtttttatc aaggagaagt tgtgccattg gcttaccaac aatattggac cttccgatga
    10861 taacagcatg ttttccctct aaatccacat ggtactctct aaacatctcc ataataccag
    10921 ctggcgtaca aggtaccatc atcggtcgtc ctgaccaaag atgacccgta ttcattgggt
    10981 ggaaaccatc aacatctttc ttaggatcta ttgctaaaat aattttttta tcgttaatat
    11041 gttgtggtaa cggaagttga actaaaatac catggatact tttatcttca ttgtattgat
    11101 ggataatatc aatcagttct tcttgagaaa tggattcaga taatcgtaaa gtctcacttt
    11161 taaaaccagc ttctaatgca gagcgttctt tatttcgaac gtacacttgg ctagctggat
    11221 tatccccaac aaggattaca gctaaaccag gtatgatacc atgctgttcc tttaaccttt
    11281 caacttttcg ccctaactca gcctgcattt tttgtgataa ggctttacca tctattaatt
    11341 cagtcattga tactccttct tcatccgttt tttctatatt atatcaaaaa atagcgatta
    11401 tgctatttta tatcgctatt cattttgtat caattttaat caatagaaaa ggcaatgaat
    11461 ccctcatcgc ctttttacat atgacttatt tagtactatt cattttagca cgacaagccg
    11521 actcaattgc attaagtttc gataaactat ctgcttctgt acaaccaatc gtataaaggt
    11581 aacattttat cttaggttct gtccctgacg gccttaaagc ataccatgaa ccctcattaa
    11641 aataatattt taaacaattt tgctttggaa aatccttata accatccttg aaatcaatag
    11701 aattctccaa tgtcatctca cctacttgta atattgggtt ctgtctaaaa tcctccataa
    11761 tacgactaat acgttcttgc ccctcagcac cctctaactc aagagaaaat tggcgctcgt
    11821 tgtaatagcc aaattcatcg taaatggttt gcaaaacgtc taaaagtgtt tgccctcgtt
    11881 ctttataata ggcagtcatt tctactacca tcattgaagc actcacagca tctttatcac
    11941 gtacaaaagt gccatagcaa aaaccaatac tttcttcata gccaaagaga taagttttat
    12001 cctttgagat atcatattca ttagcttttc cacaaatatt tttaaatcct gttaaagttt
    12061 caacagtttc aatattatat ttatctgcaa taacttttga tagatcacca gttacaatgg
    12121 attttaccaa tacaggatga tgtggcaaat tccctaaggc acatcgttgt gaaaaaatat
    12181 aataggaaag aagtgcccct atcttattac catttaagaa aatatattct cccttactat
    12241 ctttgacttc caatgctact cggtcacaat ctggatctgt ggcaagtaag acatctgcat
    12301 caactgactt tcctagagat tctgaatagg caaatgcttt aggaacttca gggtttggat
    12361 agccaactgt tgtaaaatca ggatcgggca tttcctgctc aggtactaca taaatatttt
    12421 caaaaccacg gcgtcttaaa acttcgcgca caggtaaatt tcctacgcca tttaaaggag
    12481 tataaactac tcggactgac ttatcaatgt tagtatcatt aatagttaaa ccaagcactt
    12541 cttttttata tgcttcttca atactctcat caatataact tgccaaacca cttgccagag
    12601 cctcttcaaa gggtatttgc ttaatttgct gataatcggt tatagcatcc atatgattgg
    12661 caatttggtc agcaatatca tctaaaatct gagatccttc tttccaatat gccttataac
    12721 cattataagc ttgaggatta tgactagcag taaccatcac acccgaaaca catcctagag
    12781 cacgaatagc atatgagcac attggtgttg ggcgaatccc tttataaata taagacttaa
    12841 taccatttgc tgccataatg gaacaagtta attctgcaaa ttccttagat tgataacgga
    12901 catcataact aactgcaatt ccacgtgcaa tagcttcagg gccatgatca ataatcgtat
    12961 tagctaatgc ttgtgctgct ttccccacca tataagtatt catacgattg gttcctgctc
    13021 caagtttacc tctcaatccc gccgttccaa attctaatgt tttgtaaaaa cgatcctgaa
    13081 tttcagattc atctccttta atagcttcta aatctgactt aatatcttta ccgagtgagt
    13141 cgttttctaa ccactcttga taaatctctt tataattcat atgagacatc ctattctaag
    13201 accaaaatgg taaaaatcag ctccatgata agagatttta gttcttcagg agctaactga
    13261 atagtatcaa attaacctta taaagtcaac ttattttata aattaactac acttaatata
    13321 tttaaagcat ctaaattttt ttcatcaccc tatcattctt aatctacatt atatatagac
    13381 ttaccaactt ccaaacaata cagtaacaat ttactcataa caatggaata ataaaagtct
    13441 aaaacaagtt gttttagaca atgaaattat aatactttac ttaagaagtc tcttgtgcgg
    13501 atttcttttg tctgctcaaa tactttctta ggggtccctt gctcaacaat aatccctgca
    13561 tccataaaaa tgacacgatc cgctacttca cgtgcaaaac ccatttcatg agtgacaata
    13621 accatcgtca taccagattt agctaaatct tgcataacag tcaagacttc acctaccatt
    13681 tcaggatcaa gagctgaagt aggttcatca aaaagaagga catcaggatt cattgcaaga
    13741 cctcttgcaa tagcaatccg ttgttgttgt cctccagata agcttgctgg ataagcatta
    13801 gccttctctt tgagtccaac tttttcaagt agctcgtatg cttttgtctg agcatcaagc
    13861 ttagaaagtc cctttgtctt aataggtgat aaagtaatat tttctagtac agtcatattg
    13921 ggaaatagat tgaactgttg aaaaaccatg cccatttttt cgcgcatttt aaaaatatca
    13981 ttctttttgt ctgttatatc aatcccttca aaagtcactg ttccctttgt tggtacttcc
    14041 aagagattca ttgttcttaa aaatgttgac ttaccagagc cagaagggcc aataataacc
    14101 accacttctc cttgatgaat atccaagtca atgcctttta aaacctcatt tttcccaaaa
    14161 cttttatgaa gtccttgtat tgaaataatt ggctcaacca tctattttta cccctttccc
    14221 aagatatttc tccattggtt ttaacaaagc tgagagaatc gttgtcaaca ttaaatagta
    14281 aaatgctgca aataataacg gtgcaactgg tgagtaagta gccgttacaa ctgattgtgc
    14341 tccgttccat aattccatga caccaatagt ttgaaggaga gcactatctt taataattgt
    14401 aataaattca ttccctagag caggtaaaat atttttaaaa gcttggggta agataacata
    14461 gcgaagtgta tttttaggtc gaatccccaa cgagtaagct gcttctattt gtccagatgg
    14521 tacagcctca atccctgcac gtacaatttc cgaaatatag gcaccactat ttaaggaaat
    14581 gataatgata ccaggtaaaa gtcgtgtaaa atctaaatct aaaacaccaa agctaattgt
    14641 tggtaaattg ttaaaatgca tccatgcgaa agcaatcata atttgaacta ccatcggtgt
    14701 cccacgaaat acccatacat agaaattagc taatattgtg agaaaatgta aattagtacg
    14761 ctttactaaa gcaattaaca cgcctataat agttccaaaa aaaacaacac atgttgaaat
    14821 cataatggtt accatcacac cataattaaa ataggaccaa tattgtggta aaaatgaaaa
    14881 attcattgac atctcctaaa aaagtaattg ccaatagtct atcacacaaa gaataaaaat
    14941 acaagtttaa ataaatattt ttgtttttat tctat
//
LOCUS       DATROQ010000044        14102 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.045, whole genome shotgun sequence.
ACCESSION   DATROQ010000044 DATROQ010000000
VERSION     DATROQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14102
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.045"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            78..2642
                     /locus_tag="VBC94_001217"
     CDS             78..2642
                     /locus_tag="VBC94_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GBS Bsp-like repeat-containing protein"
                     /protein_id="HEO6979492.1"
                     /translation="MRPIVRKKMYKKGKFLVIAGIVTILGGSAILGQDVKAEQAEAVT
                     STISEKTDSSQTISDISKLTLPVNSSEAMKNSAEPLIKTGFATSVSSNPREIAATPVK
                     TFDASSKVVVKASTAEHSANQTNSNVNQVANDSEVITQQNSTKQLPTVTYSAHVQDIG
                     WQKSVDNATVSGTVGQEKQVEAIKLSIKAPEGITGKLSYKTYVKGQGWQPSVESGQVS
                     GTVGQSRPIEALSINLTDNLQKLYDVYYRVHVQDIGWMAWAKNGAYAGTLGMSKRLEA
                     YKVKFTLKGQSVLTPTILKEERPVLNYQVKVGQNGWQSNKLEGQMAGTLGESKALDGV
                     KFTLSTLKYGDILYRTHVQDKGWGEILGSQTSKDYQGKRLEAIQLDLSGNLKQNYDIY
                     YRAHVQDKGWMAWQKNNSVAGTVGESKRLEALEVKLVAKASDFSSQTNHSFLEMKHPG
                     LSYQTYLQKDGWKPTVLEGQLGGSIGLSKSIKAIKLNLGSTALGNIEYRTFLNGSGWQ
                     TVVNSGRESNVPNESQQVEAIQMRLTGLLSKTYSVMYRVHMQDYGWQDWTYNNDIAGS
                     TNQGKRIEAIEIKLVETAKMPITVQTTYKGTGNYNVRVTNVISPGNLKIAIWSDKNNQ
                     DDLKWYTVTPKNHEARLTFNVTNHRDNGKYFIHVYQENASNQKQLLVKTSLQVAHSNY
                     NTPYFSQRDGRWASRRYGMATLGETGCVPTSLAMILSSLKGETVLPTQIADYLYHKTV
                     EFNRGVQGTTSRGILKAAKEWGVTATALGTQANLVQALKDGYHVLAAVQNNVFVLHGS
                     HEIVLKGYNNGLTHVSDPYTPSLSGWYPISQLWKEQSYYSEDRIDIGAPFVKVTDA"
     gene            2765..3283
                     /locus_tag="VBC94_001218"
     CDS             2765..3283
                     /locus_tag="VBC94_001218"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEO6979493.1"
                     /translation="MDLNNYIASIENYPQEGITFRDISPLMADGKAYSYAVREIVQYA
                     ADKDIDMIVGPEARGFIVGCPVAYALGIGFAPVRKPGKLPREVISADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVVAGCAFLVELDGLNGRKAIE
                     GYDTKVLMNFPG"
     gene            3401..4081
                     /locus_tag="VBC94_001219"
     CDS             3401..4081
                     /locus_tag="VBC94_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEO6979494.1"
                     /translation="MTYLEQYQSGQLTLPSALFFHFKSIFKTADDFLVWQFFYLQNTT
                     NLSDLTPSRIATSLDKTVADINRSISNLTSQGLLDVKTIELNHEIEIIFDTSPVFAKL
                     DKLFEEDNQVIIDNKTADSNRLKDLVGDFERELGRLLSPFELEDLQKTLQEDQTDPDI
                     VRAALREAVFNGKTSWNYINAILRNWRREGLTTLRQIEERKQAREDNQMKDLAISDDF
                     KNAMNLWE"
     gene            4185..4868
                     /locus_tag="VBC94_001220"
     CDS             4185..4868
                     /locus_tag="VBC94_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEO6979495.1"
                     /translation="MDLQLSKRLQKVANYVPKGARLLDVGSDHAYLPIFLLQMGYCDF
                     AIAGEVVNGPYQSALKNVSEHGLTSKIDVRLANGLSAFEEADNIDTIAICGMGGRLIA
                     DILNNDIDKLQHVKTLVLQPNNREDDLRKWLAANDFEIVAEDILTENDKCYEILVVKH
                     GHMNLTAKELRFGPFLLSNNTTVFKEKWQNELNKLTFALNSIPNSKMEERAILEDKIQ
                     DIKEVLDES"
     gene            4858..5646
                     /locus_tag="VBC94_001221"
     CDS             4858..5646
                     /locus_tag="VBC94_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEO6979496.1"
                     /translation="MKARELIDVYETYCPQELSMEGDISGLQIGSLDKEIKTVMVALD
                     VRETTVAEAIERQVDLLIVKHAPIFRPLKDLVATPQNKIYIDLLKSDIAVYVSHTNID
                     IVPNGLNDWFCELLDIQYPDILSETSNGYGIGRIGDVRPQSFEFFAWKIKDVFGLDSV
                     RLVSYDKSNPEIQRVAICGGSGQSFYKEAIAKGADVFVTGDIYYHTAQEMITNGLLAI
                     DPGHHIEVLFVSKIATMIEQWKLEKGWDISVLESKAPTNPFYHM"
     gene            5655..6758
                     /locus_tag="VBC94_001222"
     CDS             5655..6758
                     /locus_tag="VBC94_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEO6979497.1"
                     /translation="MKKIAIIGAGAVGATLAYYLSKEKDIQVTVFDYGVGQATKAAAG
                     IISPWFSKRRNKAWYRMARLGADFYSKLVTDLQKDGFETKFYQQTGVFLLKKDESQLE
                     SLFALADKRRLESPLIGDLQILNKSEANTHFPELDGYEQLLYASGGARVEGADLTRIL
                     LEASGVNVIKDEVHFTITDNGFRVQGIDFDKLVLASGAWLAKILDEHNYQVDIRPQKG
                     QLRDYYFSNINTGKYPVVMPEGELDIIPFDNGKVSVGASHENDMAFDLNIDFKVLDKF
                     EEQAIGYFPQLKKADTTSERVGIRAYTSDFSPFFGPVPCMEGAYAASGLGSTGLTVGP
                     LIGYELCQLILNKENQLNLEDYDITKYVKKISR"
     gene            6817..7686
                     /gene="rfbA"
                     /locus_tag="VBC94_001223"
     CDS             6817..7686
                     /gene="rfbA"
                     /locus_tag="VBC94_001223"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEO6979498.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSVLMLA
                     GIKEILIISTPQDLPRFEDMLGDGSELGISLSYAEQPSPDGLAQAFIIGEEFIGDDHV
                     ALVLGDNIYHGPGLSAMLQRAASKESGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PAQPKSNYAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRMQNVQVANLEEIAYRMGYITREQVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            7686..8279
                     /locus_tag="VBC94_001224"
     CDS             7686..8279
                     /locus_tag="VBC94_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEO6979499.1"
                     /translation="MTEQFFDKELTCRPIEAIPGLLEFDITVRGDNRGWFKENFQKEK
                     MIPLGFPESFFEADKLQNNISFNKKNTLRGLHAEPWDKYVSIADEGRVIGTWVDLREG
                     DSFGNVYQTIIDASKGIFVPRGVANGFQVLSDKAAYTYLVNDYWALELKPKYAFVNYA
                     DPNLGIQWENLEEAEVSEADKNHPLLKDVKPLKKEDL"
     gene            8486..9532
                     /gene="rfbB"
                     /locus_tag="VBC94_001225"
     CDS             8486..9532
                     /gene="rfbB"
                     /locus_tag="VBC94_001225"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEO6979500.1"
                     /translation="MTEFKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGN
                     RANIEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLEDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGNGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLY
                     GEGKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKD
                     AYDHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKD
                     AVEAKYAKTQEVIK"
     gene            complement(9584..12802)
                     /gene="hylB"
                     /locus_tag="VBC94_001226"
     CDS             complement(9584..12802)
                     /gene="hylB"
                     /locus_tag="VBC94_001226"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_192884539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEO6979501.1"
                     /translation="MEIKKKHRIMLYSALILGTILVNNSYQAKAEELTKTTSTSQIRD
                     TQTNNIEVLQTESTTVKETSTTTTQQDLSNPTASTATATATHSTMKQVVDNQTQNKEL
                     VKNGDFNQTNPVSGSWSHTSAREWSAWIDKGNTADKSPIIQRTEQGQVSLSSDKGFRG
                     AVTQKVNIDPTKKYEVKFDIETSNKAGQAFLRIMEKKDNNTRLWLSEMTSGTTNKHTL
                     TKIYNPKLNVSEVTLELYYEKGTGFVTFDNISMKAKGPKDSEHPQPVTTQIEKSVNTA
                     LNKNYVFNKADYQYTLTNPSLGKIVGGILYPNATGSTTVKISDKSGKIIKEVPLSVTA
                     STEDNFTKLLDKWNDVTIGNYVYDTNDSNMQKLNQKLDETNAKNIEAIKLDSNRTFLW
                     KDLDNLNNSAQLTATYRRLEDLAKQITNPHSTIYKNEKAIRTVKESLAWLHQNFYNVN
                     KDIEGSANWWDFEIGVPRSITGTLSLMNNYFTDAEIKTYTDPIEHFVPDAEYFRKTLV
                     NPFKALGGNLVDMGRVKIIEGLLRKDNTIIEKTSHSLKNLFTTATKAEGFYADGSYID
                     HTNVAYTGAYGNVLIDGLTQLLPIIQETDYKISNQELDMVYKWINQSFLPLIVKGELM
                     DMSRGRSISREAASSHAAAVEVLRGFLRLANMSNEERNLDLKSTIKTIITSNKFYNVF
                     NNLKSYSDIANMNKLLNDSTVATKPLKSNLSTFNSMDRLAYYNAKKDFGFALSLHSKR
                     TLNYEGMNDENTRGWYTGDGMFYIYNSDQSHYSNHFWPTVNPYKMAGTTEKDAKREDT
                     TKEFMSKHSKDAKEKTGQVTGTSDFVGSVKLNDHFALAAMDFTNWDRTLTAQKGWVIL
                     NDKIVFLGSNIKNTNGIGNVSTTIDQRKDDSKTPYTTYVNGKTIDLKQASSQQFTDTK
                     SVFLESKEPGRNIGYIFFKNSTIDIERKEQTGTWNSINRTSKNTSIVSNPFITISQKH
                     DNKGDSYGYMMVPNIDRTSFDKLANSKEVELLENSSKQQVIYDKNSQTWAVIKHDNQE
                     SLINNQFKMNKAGLYLVQKVGNDYQNVYYQPQTMTKTDQLAI"
     gene            13019..13501
                     /locus_tag="VBC94_001227"
     CDS             13019..13501
                     /locus_tag="VBC94_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO6979502.1"
                     /translation="MTKLATICYIDNGKELLLLHRNKKENDVHEGKWISVGGKLEAGE
                     TPDECAKREILEETHLTVKKMDFKGVITFPEFTPGHDWYTYVFKVTDYEGELISDDES
                     REGTLEWVPYDQVLSKPTWQGDYEIFKWILEDVPFFSAKFVYDEHQNLIEKTVNFYEK
                     "
     gene            13491..13952
                     /locus_tag="VBC94_001228"
     CDS             13491..13952
                     /locus_tag="VBC94_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6979503.1"
                     /translation="MKNRDFRVRDQLETFGVRVSALIIENQKLLLIYAPHLYKYYLPG
                     GALQVGEDSNKAVAREVLEEIGLHSQVGDLAYIVENQFNIKRHHYHSVEFLYFVNLLG
                     QAPESIKEGTHKRHFVWLPIKELTKIDCNPNFLAQDLIEWPGHVVHKIVQN"
BASE COUNT         4574 a         2215 c         2844 g         4469 t
ORIGIN      
        1 aatattatta aaatataata aaattaagaa aataatggaa tttttgataa agaatattaa
       61 aggtgaggat aaaatctatg agacctatcg ttagaaaaaa gatgtacaaa aaaggtaaat
      121 ttttggtcat tgcaggtatc gttactattt taggtggtag tgcaattctt ggtcaagatg
      181 tcaaggcaga acaggcagaa gcagtaactt caacgatctc agagaaaact gatagcagtc
      241 agactatttc ggatatttcc aagttaacgt tacctgttaa ttcctcggag gctatgaaaa
      301 atagtgcaga gcctttaata aaaacaggct ttgctacatc tgtttcatct aatcctagag
      361 agatagcagc taccccagtc aaaacctttg atgctagttc aaaagttgtt gtaaaagcta
      421 gtactgctga gcactcagca aatcaaacaa attctaatgt taatcaagta gctaatgata
      481 gtgaagttat tactcaacaa aattcaacaa agcaattacc aacagtgact tactctgctc
      541 atgttcaaga tattggatgg caaaagtcgg ttgataatgc aacagtttca ggaacagtag
      601 gacaggaaaa gcaggttgag gcaataaaat tgtctattaa agcccctgaa ggaataacag
      661 gaaaacttag ctataaaact tatgttaaag gacaaggttg gcaaccttca gtagagagtg
      721 gtcaagttag tggtactgtt ggtcagagta gacctattga agcactaagc attaacctta
      781 ctgataattt acaaaaatta tatgatgtct attatcgtgt tcatgttcaa gatatcgggt
      841 ggatggcttg ggctaaaaat ggagcttacg ctgggacttt gggaatgtca aagcgccttg
      901 aagcttacaa agtaaagttt acccttaaag gacaatctgt tttaacacca actatactta
      961 aggaagagag acctgtctta aattatcagg ttaaggtagg ccaaaatggt tggcaatcta
     1021 ataaacttga aggacaaatg gcgggaactt taggagaatc caaagcccta gatggagtaa
     1081 aattcactct tagcaccttg aagtatgggg acattctcta tcgtactcat gtacaagata
     1141 aaggttgggg agaaatttta ggtagtcaaa caagtaagga ttatcaagga aaaagactag
     1201 aagctattca attggactta agtgggaatt taaaacaaaa ttatgatatt tattatcgcg
     1261 ctcacgtcca agataaaggt tggatggctt ggcaaaaaaa taatagtgta gcaggtacag
     1321 tcggagaatc gaaacgttta gaggctttgg aagtaaaact agtagctaaa gcttcagatt
     1381 ttagttccca aacaaatcat tcattcttag aaatgaagca tcctggatta agttatcaaa
     1441 cttatcttca aaaagatggt tggaaaccaa cggttttgga aggccaatta ggaggtagta
     1501 ttggactttc aaaatctata aaagccatca aacttaactt aggtagtaca gctctaggaa
     1561 atatagaata tagaactttt cttaatggtt caggttggca aactgttgtt aattcaggga
     1621 gagaaagtaa tgtaccaaat gaatctcaac aagtagaagc tattcagatg cgattgacag
     1681 gtctgctatc gaaaacctat agtgtgatgt atcgagttca catgcaagat tatggttggc
     1741 aggattggac ttataacaat gacatcgctg gtagtactaa ccaaggaaaa cgaattgaag
     1801 caatcgaaat taaattagtt gagactgcta aaatgcctat aactgtccaa acgacctata
     1861 agggaactgg aaactataat gtacgagtga caaatgttat tagtcctggg aatttaaaaa
     1921 ttgctatttg gtcagataaa aacaatcagg atgaccttaa gtggtatacc gtcacaccca
     1981 aaaatcatga agctagattg acatttaatg ttacaaatca tagagacaat ggtaaatact
     2041 ttatccatgt ttatcaggaa aatgcttcta atcaaaaaca attattggta aaaacttctc
     2101 tacaagtcgc acattcaaat tataatactc cttactttag ccaacgtgat ggaaggtggg
     2161 caagtcgaag atatggtatg gcaactttag gagaaactgg ttgtgtacca acttctctag
     2221 ctatgatttt gtcttctttg aaaggagaaa ctgttttacc aacgcaaatt gcagattacc
     2281 tttaccataa gacagttgaa tttaatcgtg gtgttcaagg aacaacttct agaggaatct
     2341 taaaagcagc caaagaatgg ggtgtaacag caactgcatt aggaactcaa gctaaccttg
     2401 tacaagcgtt gaaggatggt tatcatgtcc tagcggcagt acaaaataat gtttttgttc
     2461 ttcatggaag tcatgaaatc gttctcaaag gatataataa cggactgaca catgtctctg
     2521 acccttacac accgtccttg tctggttggt atccaatttc acaactttgg aaagaacaga
     2581 gctattatag tgaggataga attgatattg gagcaccttt tgtcaaggta acagatgcct
     2641 aatttttaaa accatatgca aaactagcat atggttttaa agtttccagt actattttat
     2701 ttaattttat gatataatga agagtaaaac attttagggg tttcccctta gaaagacaag
     2761 tgaaatggat ttaaataatt acatcgcttc aattgaaaac tatcctcaag aaggaattac
     2821 tttccgtgat atctccccat taatggctga tggtaaagca tacagctatg ctgttcgtga
     2881 aattgtacaa tatgctgctg ataaagatat tgatatgatt gttggaccag aagctcgtgg
     2941 ctttattgtt ggttgcccag ttgcttatgc tcttggtatt ggttttgctc cagtccgtaa
     3001 accaggtaaa ttaccacgcg aagttatttc agctgactat gaaaaagaat atggtcttga
     3061 tacattgaca atgcacgcag atgctattaa accaggtcaa cgtgttttaa ttgttgatga
     3121 ccttttagct actggtggaa cagtaaaagc gacaattgaa atgattgaaa aacttggtgg
     3181 cgttgtcgca ggctgtgctt tcctagttga acttgatgga ttaaatggtc gtaaagctat
     3241 tgaaggttat gatactaagg tattgatgaa ctttccagga taaggcctat tcaagataga
     3301 tgaatgataa gggcgcctct aagtgctatg ataatagttg agaggtgctt tttgttacac
     3361 atttcataat tttaggacga tagagaggat ttgaaaggtt atgacttatt tggaacaata
     3421 tcagtcaggg caattgactc taccaagtgc tttatttttt cactttaaat ctatttttaa
     3481 gacagcagac gattttttag tttggcaatt tttctattta caaaatacaa ctaatttatc
     3541 cgatctaaca ccgagtcgta ttgcgacatc acttgataaa acagtggcag atatcaatcg
     3601 ttctatatcg aatttaacaa gtcaaggttt actagatgtc aaaacaattg agttgaatca
     3661 cgaaatcgag attatctttg atacaagccc tgtgtttgct aaattagata aacttttcga
     3721 agaggataat caagtaataa tagacaataa aaccgctgat tcgaatcgtc tgaaagattt
     3781 agtaggagac ttcgaacgtg aattaggtcg cttattaagt ccttttgaat tagaagattt
     3841 acaaaagaca ctgcaagaag atcagacaga tcctgatatt gttagggcag ctcttagaga
     3901 agcggttttc aatgggaaaa caagttggaa ttatatcaat gctatccttc gcaactggcg
     3961 gcgtgaagga ttgacgacac ttcgacaaat tgaagagaga aaacaagcac gtgaagataa
     4021 ccaaatgaaa gatttagcga tttcggatga ttttaaaaat gcaatgaatt tatgggaata
     4081 agtttactag gaggagacag aatggttgat acattaggtc tgctcctttt cttttgatgt
     4141 taatgatgtt atacttttaa cagagaaaaa ctgaaggaaa tacaatggat ttacaattat
     4201 caaaacgatt acaaaaagtt gcaaactatg ttccaaaagg agctcggtta ttagatgtag
     4261 gaagtgatca tgcttactta cctatttttt tgttgcagat gggatactgt gattttgcca
     4321 ttgctggtga agtggtgaat ggaccgtatc agtctgcgtt gaaaaacgtt tcagaacatg
     4381 gtctcacttc aaagatagat gttcgcttag caaatggttt gtcagctttt gaagaagcag
     4441 ataatattga tacgatagct atctgtggaa tgggtggtcg tttgattgct gatattttga
     4501 ataacgatat agacaaactt cagcacgtta aaacactagt cttacaacct aataatcggg
     4561 aagatgattt aagaaaatgg ttagctgcaa atgattttga aatagtagca gaagatatct
     4621 tgactgaaaa tgacaaatgc tatgaaattt tagttgttaa gcatggtcat atgaatttaa
     4681 ctgctaaaga attgcgattt ggtccatttt tattatccaa taacacaaca gtttttaaag
     4741 aaaaatggca aaatgagctt aataaattaa cttttgcttt aaactctatt cccaatagta
     4801 aaatggaaga acgtgctatt ttggaagata aaatacagga tataaaggag gttcttgatg
     4861 aaagctagag aacttattga tgtatatgaa acttattgtc cacaggaatt atctatggaa
     4921 ggagatattt caggtcttca aattggatct ttagataaag aaataaagac agtcatggta
     4981 gctttagatg ttcgagagac tacagtagct gaagcaattg aacgtcaagt agatcttctt
     5041 attgtaaagc atgctcctat ttttcgtcca ttaaaggatt tggtagcaac accgcaaaat
     5101 aagatttata tagaccttct taaatcagac attgctgttt atgtgagcca tactaatata
     5161 gatatcgttc ctaatggact taacgactgg ttttgtgaat tattagatat tcaatatccc
     5221 gatatattat ctgagacaag taatggttat ggtattggtc gcattggtga tgttagaccc
     5281 cagagttttg aattttttgc atggaaaatt aaagatgtgt ttggtttaga tagcgttcgt
     5341 ttagtttcat atgataaatc taatcctgaa attcaacgag ttgctatttg tgggggtagt
     5401 gggcaaagtt tctacaaaga agctattgca aaaggagcag atgtttttgt aacgggagat
     5461 atttattatc atactgcaca agaaatgatc acaaatggtt tgcttgcgat tgacccaggc
     5521 catcatattg aggttttatt tgtttctaaa atagctacta tgattgagca gtggaaactt
     5581 gaaaaaggct gggatatttc agttttagag agtaaagctc caacaaatcc cttttaccat
     5641 atgtaggagc tattatgaaa aagatagcaa ttattggtgc aggagcagtc ggagcgacac
     5701 tagcctatta cttatcaaaa gaaaaggata ttcaggttac tgtttttgat tatggagttg
     5761 gacaagcaac taaggctgct gcggggatta ttagcccatg gttttcaaaa cgtcgtaata
     5821 aagcctggta tcgcatggcg aggttggggg ctgattttta ttcaaagcta gtcacggatt
     5881 tacaaaaaga tggttttgaa acaaaatttt atcagcaaac tggtgttttc ttattaaaaa
     5941 aggacgagag tcagttagag tcattgtttg cactagcaga taaacgtcga ctagaatctc
     6001 ctctaatagg tgatttacaa atactaaata aaagtgaagc aaatactcac tttcctgagt
     6061 tagatggcta tgaacagtta ctttatgcga gtggaggagc gcgagtagaa ggggcggact
     6121 taacacgaat attgttagaa gcaagtggtg ttaatgtcat caaggatgaa gttcacttta
     6181 cgataactga taatggcttt cgtgttcaag gaattgattt tgataaactt gttttagcga
     6241 gtggagcctg gctagctaag atattagatg agcacaacta tcaggtagat attcgccctc
     6301 aaaaagggca attgcgagac tattatttta gcaatattaa tacaggaaaa tatcctgttg
     6361 taatgccaga aggcgagcta gatattatcc catttgataa tggtaaagtc tctgtaggtg
     6421 caagtcacga aaatgatatg gcttttgatt taaacattga ttttaaagtc ttagacaagt
     6481 ttgaagagca agctattggc tattttccgc aattaaaaaa agcagatacg acttctgaac
     6541 gtgttggaat tcgtgcctat acaagtgatt tttctccatt ttttggccct gtcccttgta
     6601 tggagggtgc atatgctgca agtggattag gatcaactgg cttaacagtg ggcccattaa
     6661 ttggttatga attatgtcaa ttaatcttaa ataaagaaaa tcagctaaat ttagaggatt
     6721 acgatattac gaaatacgtg aaaaagatta gtagatgata gcgtttaaaa aaagtgttat
     6781 aatatgtagg attaatataa ataaggagac cttactatga aaggtattat tttagcggga
     6841 ggttcaggta ctcgcttgta cccattaact cgtgctgcat caaaacaatt gatgccaatt
     6901 tatgataaac caatgattta ttatccattg tcagttttga tgttggcagg aataaaagaa
     6961 atactgatta tttctactcc acaagatttg cctcgctttg aagatatgct tggagatggt
     7021 tcagaactag gaatctctct ttcttatgct gaacaaccaa gcccagatgg gttagcacag
     7081 gccttcatta tcggtgaaga atttattggc gatgatcacg ttgccttagt attaggtgat
     7141 aatatttacc atggaccagg tttatctgca atgctacaac gtgcagctag taaagaaagt
     7201 ggagcaaccg tctttggcta tcaagtaaaa gatccagaac gttttggtgt tgtcgagttt
     7261 gatactgata tgaatgccat ttctattgaa gaaaaaccag cgcaacctaa gtctaattat
     7321 gctgtcacag gtctttattt ctatgataac gatgtggttg aaatagctaa aaatattaag
     7381 ccaagtcctc gtggagaact tgaaattact gatgttaaca aagcctacct tgatagaggt
     7441 gatttatcag ttgagttaat gggacgtgga tttgcgtggt tagacactgg aactcacgaa
     7501 agtcttcttg aagcagcaca atatattgaa acagtacaac gcatgcaaaa cgttcaagtt
     7561 gctaaccttg aggaaattgc ttaccgcatg ggctacatca ctcgtgaaca agtccttgag
     7621 ttggcacaac cacttaagaa aaatgagtat ggtcaatacc tacttcgttt gattggagaa
     7681 gcctaatgac tgaacaattt tttgataaag aattaacttg tcgcccaatt gaagccattc
     7741 ctggattgtt ggaattcgat attaccgttc gtggagacaa ccgtggatgg ttcaaagaga
     7801 actttcaaaa agaaaaaatg ataccgcttg gtttcccaga aagcttcttt gaggcagaca
     7861 aactacaaaa taatatttcg tttaacaaaa aaaatacttt gcgaggtctc catgcagagc
     7921 cttgggataa atatgtttcg atcgctgatg aaggacgtgt gatcggtact tgggttgacc
     7981 tccgtgaagg tgacagtttt ggtaacgttt accaaacgat tatcgatgcc tcaaaaggta
     8041 tttttgttcc acgcggcgtt gctaatggtt tccaagttct ttcagataaa gcagcttata
     8101 cttatctcgt taacgattat tgggcacttg aactcaaacc aaaatatgct ttcgttaact
     8161 atgcagatcc aaatctaggc attcagtggg aaaaccttga agaagcagaa gtctcagaag
     8221 cagataagaa tcacccactt ctcaaagatg taaaaccttt gaagaaggaa gatttgtaat
     8281 tttcaacttc ttaaaaggca ttaaccttag caagtaaaaa cgaattagca atgttgtttg
     8341 acgcaagggt gaaaggaaac atgcaaaagg cctgttccct cagggatttg aaatattgat
     8401 ttcttaggca tgacattcca tagaactact tctaacagaa cagtatcctt caaacagcaa
     8461 aaagaaaaaa tttttcggag aaaaaatgac tgaatttaaa aacattatcg taactggtgg
     8521 agctggtttc atcggatcaa actttgtaca ctatgtttac aataaccacc cagatgttca
     8581 tgtaactgtc cttgataaat tgacatatgc aggtaaccgt gctaacattg aagccattct
     8641 tggtgatcgt gttgagttag ttgttggtga catcgctgac gctgaattgg tagacaaatt
     8701 ggctgccaaa gcggatgcta ttgttcacta tgcggctgag agtcacaacg ataactcatt
     8761 ggaagatcca agtccattta tccatacaaa ctttatcgga acttacactt tgcttgaagc
     8821 agctcgtaaa tacgatatcc gtttccacca cgtgtcaact gatgaagttt atggagatct
     8881 tccacttcgt gaagaccttc caggaaatgg tgaaggacca ggtgaaaaat tcactgctga
     8941 aacaaaatac aatccatcat caccttactc atcaactaag gcagcttctg accttatcgt
     9001 taaggcatgg gtacgttcct tcggcgtaaa agcgaccatt tcgaactgtt caaataacta
     9061 tggaccatac caacacatcg aaaaattcat tccacgtcaa attacaaata tcttggcagg
     9121 aatcaaacca aaactttatg gtgaaggtaa aaacgtccgt gactggatcc acacaaatga
     9181 ccattcaaca ggtgtctggg ctattttgac taagggtcgt attggtgaaa cataccttat
     9241 tggtgccgac ggtgagaaaa acaacaagga ggttcttgaa cttatccttg agaaaatggg
     9301 tcaaccaaaa gacgcttacg atcacgtaac tgaccgtgct ggtcatgatc ttcgttacgc
     9361 tatcgattct acaaaattgc gtgaagaact cggctgggaa ccacaattta caaacttttc
     9421 agaaggtttg gaagaaacta ttaaatggta tacagaaaat gagacatggt ggaaagcaga
     9481 aaaagatgct gtagaagcca agtatgctaa aactcaagaa gtcattaagt aattgaacca
     9541 aaaaacctat cattttgatg ataggttttt tatgtacggc tagttagata gctaattggt
     9601 ctgtttttgt catggtttga ggttggtaat agacattttg atagtcatta ccaacttttt
     9661 gtactaggta aagtcccgct ttattcattt tgaattgatt gttaatgaga ctctcttgat
     9721 tatcgtgttt gataacagcc caagtttgac tgtttttatc atagataact tgttgttttg
     9781 aactattttc tagtaattct acttctttgc tgttggctaa tttatcaaaa cttgtgcgat
     9841 caatgtttgg aaccatcatg taaccatagc tatcaccttt gttgtcatgc ttttggctta
     9901 tagtgataaa aggattgcta acgattgagg tatttttaga agtacgatta atgctgttcc
     9961 aagtacctgt ttgctctttg cgttcaatat caatagtgct atttttaaag aagatataac
    10021 caatattgcg accaggttct tttgattcta aaaagacact ttttgtatct gtaaattgtt
    10081 gagaacttgc ttgttttaaa tcaatagttt ttccattgac gtatgtagta taaggtgttt
    10141 tagaatcgtc ttttcgttga tcaattgttg tagaaacatt tccaatgccg ttagtattct
    10201 tgatgttgct acctaaaaag acaatcttat catttaagat aacccaacct ttttgtgctg
    10261 ttaaggtgcg atcccagtta gtaaaatcca tagcggcaag agcaaagtga tcattaagtt
    10321 tgacggaacc aacaaagtca gatgttcctg taacttgacc ggttttttct ttagcgtctt
    10381 tgctatgttt gctcatgaat tccttagtgg tatcttcacg cttagcatct ttttcagttg
    10441 ttccagccat tttataagga ttgacggttg gccaaaaatg attactataa tgagattgat
    10501 cactattata aatatagaac ataccatctc cggtatacca accacgtgta ttttcatcat
    10561 tcattccttc atagttgagg gtacgtttag aatgtaatga aagcgcgaaa ccaaagtctt
    10621 tcttggcatt ataataagct aagcggtcca tgctattaaa ggttgataaa ttacttttta
    10681 aaggtttagt agcgactgta ctgtcattaa gcagcttatt catgttggca atatcggaat
    10741 acgatttgag gttattgaag acattgtaga atttatttga agtgataatc gttttaatag
    10801 ttgatttgag gtctaagttt cgctcttcat tagacatgtt agccaatctg aggaaacctc
    10861 tgagaacttc aactgcagcc gcatgcgaag aagctgcctc tctactaatt gagcgtccac
    10921 gactcatatc cattaactca ccttttacaa ttaaaggtaa aaatgattga ttaatccatt
    10981 tataaaccat atcaagttct tgattagaga ttttatagtc agtttcttga atgataggca
    11041 gcaattgtgt caaaccatct atcagaacat taccataggc gccagtataa gcaacatttg
    11101 tatggtcgat gtaagaaccg tcagcataga agccttcagc tttagtagca gtagtaaaaa
    11161 gattttttag agaatgagaa gttttttcga taatagtatt gtctttacga agtaaacctt
    11221 caatgatttt aacgcgcccc atatctacta gattaccacc aagggcttta aatggattga
    11281 caagcgtttt acggaaatat tctgcatcag gaacaaagtg ttcaattggg tcggtataag
    11341 tttttatttc agcgtcagtg aagtagttat tcatgagaga tagggtacct gtaattgagc
    11401 gaggaacacc gatttcaaaa tcccaccaat tggcagagcc ttctatatct ttattaacat
    11461 tgtagaagtt ttgatgaagc caagccagac tctcttttac agtacgaata gctttttcat
    11521 ttttgtaaat agtagagtgt ggattggtga tttgtttagc taaatcttcc aaacgacgat
    11581 aagtagcggt taactgtgct gaattattga gattatctaa atctttccaa aggaaagtgc
    11641 gattagaatc cagtttgata gcttcgatgt ttttggcgtt agtttcatct aatttctgat
    11701 taagcttttg catgttcgaa tcattagtat cgtaaacata attaccaata gtcacgtcgt
    11761 tccatttgtc gaggagtttt gtaaagttat cttctgttga agctgtaact gataacggta
    11821 cttctttaat tattttgcca gatttatcag atattttaac agttgttgaa ccagtagcgt
    11881 ttggatacaa tattccacca acaattttcc caagagacgg attggttaga gtatattggt
    11941 agtcagcttt attaaaaacg taatttttgt ttaaagccgt attaacgctt ttttcaattt
    12001 gtgttgtgac gggttgtgga tgctctgagt ctttagggcc ttttgctttc attgatatat
    12061 tatcaaaagt aacaaaacca gttccttttt cataataaag ttcaagtgtc acctcggaga
    12121 catttaactt tgggttatat atctttgtta aggtatgttt gttagtagta ccgctggtca
    12181 tctcagaaag ccaaagtcgc gtattgttgt cttttttctc cataatacga aggaaagctt
    12241 gtccagcctt gttacttgtt tcaatatcaa acttaacctc atatttttta gtgggatcaa
    12301 tgttcacttt ttgtgttaca gcacctctaa agcctttgtc gctggataga cttacttggc
    12361 cttgttcggt acgttggata ataggtgatt tatcagcagt atttccttta tcaatccaag
    12421 cagaccattc cctagcgctt gtatgtgacc agcttccaga tacagggtta gtttgattaa
    12481 aatctccgtt tttcaccagc tccttatttt gagtttgatt atctactact tgtttcattg
    12541 tgctatgagt ggctgttgca gttgcagttg aagctgtggg gttagacaga tcttgttgtg
    12601 tggttgtggt gctagtctcc ttgacagtgg tactttcagt ctggagaact tcaatattat
    12661 tagtttgagt atctcttatt tgggacgttg aggtagtttt ggtaagctct tcagctttag
    12721 cttggtaact attgttaacc aatattgttc ctaaaataag ggctgaataa agcataatac
    12781 gatgtttctt tttgatttcc atttttactc cttagggttt aaaattgtaa accggttttt
    12841 cctaattata gcatgcttta aaggtttatg ataaagaacc tttaaaatgt catcaaagtg
    12901 aaaagtaaat aactggtaaa caaaataatt tgtttaatca tttgtgtgct actttgatag
    12961 taaattgcct aaataaaggc tattttttgt tataatatga gaatgagagg agtttgaaat
    13021 gactaaatta gcaacaattt gttacattga caatggcaag gaattacttc tattgcatcg
    13081 caacaaaaaa gagaatgatg ttcatgaggg aaagtggatc tcggtaggcg gtaaactgga
    13141 ggcaggtgaa actccggatg aatgtgctaa gcgtgaaatt ctagaagaaa cccatttaac
    13201 agtaaaaaaa atggatttta aaggagtcat cacttttcct gaatttacgc caggtcatga
    13261 ttggtatacc tatgtcttta aagtaacaga ttatgaagga gagttaattt cagatgatga
    13321 atcacgagaa ggaaccttag aatgggtacc atatgatcaa gttctctcta agccaacgtg
    13381 gcaaggagat tacgaaattt ttaaatggat tttagaagat gttcctttct tttctgctaa
    13441 gtttgtttat gatgaacacc aaaatttaat tgaaaagacg gttaattttt atgaaaaata
    13501 gagattttag ggttagagac cagctcgaaa cattcggagt cagagtttct gctttaatca
    13561 tagaaaatca aaaactgcta ttaatatatg ctcctcatct ctataaatac tatttgccag
    13621 gtggggcgct tcaagttgga gaagatagca ataaggctgt tgcgagagaa gtacttgagg
    13681 aaataggtct tcacagccaa gttggtgatt tagcctatat tgttgagaat cagtttaata
    13741 ttaagagaca tcattatcac tcagttgaat ttttatattt tgttaatctc ttgggtcagg
    13801 cgccagaatc tatcaaagaa ggaacgcata agcgacattt tgtatggctt ccaataaaag
    13861 aattaacaaa aattgattgt aatcctaatt ttttagcaca ggatttgata gaatggccag
    13921 gtcatgttgt gcataaaatc gttcaaaatt aactttttac tctcttgttt tgagtttaat
    13981 ttaattcaac gtgttataat aaatgagaat taaaattaga attcatcttg aaaggatata
    14041 tgaatgaatc aaccatcaga aaaagaattt aaaaatagtt tgttttttaa atggatctta
    14101 aa
//
LOCUS       DATROQ010000045        14013 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.046, whole genome shotgun sequence.
ACCESSION   DATROQ010000045 DATROQ010000000
VERSION     DATROQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14013
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.046"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            173..1045
                     /locus_tag="VBC94_001229"
     CDS             173..1045
                     /locus_tag="VBC94_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6979504.1"
                     /translation="MLKLDLKTKIKEAILIAFGVALYTFGFVKFNMANHLAEGGISGV
                     TLIIHALFGVNPALSSLLLNIPLFILGARILGKKSLLLTIYGTVLMSFFMWFWQQIPV
                     TVPLKNDMMLVAVAAGILAGTGSGLVFRYGATTGGADIIGRIVEEKSGIKLGQTLLFI
                     DAIVLTSSLVYINLQQMLYTLVASFVFSQVLTNVENGGYTVRGMIIITKESESAAATI
                     LHEINRGVTFLRGQGAYSGREHDVLYVALNPSEVRDVKEIMADLDPDAFISVINVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(1072..1380)
                     /locus_tag="VBC94_001230"
     CDS             complement(1072..1380)
                     /locus_tag="VBC94_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO6979505.1"
                     /translation="MPSEKEILDALSKVYSEEVIQADDYFRQAIFELASQLEKEGMNS
                     LLATKIDSLINQYVLTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(1468..3159)
                     /locus_tag="VBC94_001231"
     CDS             complement(1468..3159)
                     /locus_tag="VBC94_001231"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEO6979506.1"
                     /translation="MDTKHLIASEIQKVVPYMEQSAILSLLETPKNSSMGDLAFPAFS
                     LAKTLRKAPQIIASDIAEQIKSDQFEKVEAVGPYVNFFLDKAAISSQVLKQVLSDGSA
                     YATQNIGEGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLANIFDKIGYHPVKINHLGD
                     WGKQFGMLIVAYKKWGNEEAVRAHPIDELLKLYVRINAEAEIDPSVDEEAREWFRKLE
                     ANDPEATELWQWFRDESLLEFNRLYDKMNVTFDSYNGEAFYNDKMDEVLELLESKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSIYVVGNE
                     QSAHFKQLKAVLKEMDYDWSDDMTHVPFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKDKVAQAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKANFNPSNSDNYSLNDVESWEIIKLIQDFPRIIVRAADNFEPSIIA
                     KFAINLAQCFNKYYAHTRILDEDAEISSRLALCYATATVLKESLRLLGVDAPNEM"
     gene            3381..3818
                     /gene="argR"
                     /locus_tag="VBC94_001232"
     CDS             3381..3818
                     /gene="argR"
                     /locus_tag="VBC94_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO6979507.1"
                     /translation="MNKIERQKRIKRLIQSGQIGTQEEIKLHLKNEGIDVTQATLSRD
                     LREIGLLKLRSPEGKLYYSLSTATSNRFSPALRSYILKVSRASFMLVLNTNLGEASVL
                     ANFIDEKGLPEILGTMAGADTLLVICQNEDIAKVFEKELSVGL"
     gene            3875..6451
                     /gene="mutS"
                     /locus_tag="VBC94_001233"
     CDS             3875..6451
                     /gene="mutS"
                     /locus_tag="VBC94_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEO6979508.1"
                     /translation="MAKPTISPGMQQYLDIKENYPDAFLLFRMGDFYELFYDDAVKAA
                     QILEISLTSRNKNAEKPIPMAGVPYHSAQQYIDVLVELGYKVAIAEQMEDPKKAVGVV
                     KREVVQVVTPGTVVESTKPDSANNFLVAIDSQDQQTFGLAYMDVSTGEFQATLLTDFE
                     SVRSEILNLKAREIVVGYQLTDEKNHLLTKQMNLLLSYEDERLNDIHLIDEQLTDLEI
                     SAAEKLLQYVHRTQKRELSHLQKVVHYEIKDYLQMSYATKNSLDLLENARTSKKHGSL
                     YWLLDETKTAMGTRMLRTWIDRPLVSMNRIKERQDIIQVFLDYFFERNDLTESLKGVY
                     DIERLASRVSFGKANPKDLLQLGQTLSQIPRIKMILQSFNQPELDIIVNKIDTMPELE
                     SLINTAIAPEAQATITEGNIIKSGFDKQLDNYRTVMREGTGWIADIEAKERAASGIGT
                     LKIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGEMLEAREQS
                     SNLEYDIFMRVRAQVESYIKRLQELAKTIATVDVLQSLAVVAENHHYVRPKFNDEHQI
                     KIKNGRHATVEKVMGVQEYIPNSIYFDSQTDIQLITGPNMSGKSTYMRQLALTVIMAQ
                     MGGFVSADEVDLPVFDAIFTRIGAADDLISGQSTFMVEMMEANQAVKRASDKSLILFD
                     ELGRGTATYDGMALAQSIIEYIHDRVRAKTMFATHYHELTDLSEQLTRLVNVHVATLE
                     RDGEVTFLHKIEPGPADKSYGIHVAKIAGLPIDLLDRATDILSQLEADAVQLIVSAPQ
                     EAVTADLNEELDPGKQQGQLSLFEEHSNAGRVIEELEAIDIMNLTPMQAMNAIFDLKK
                     LL"
     gene            complement(6508..6711)
                     /locus_tag="VBC94_001234"
     CDS             complement(6508..6711)
                     /locus_tag="VBC94_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO6979509.1"
                     /translation="MTQGTVKWFNSEKGFGFISSETGTDVFAHFSEIKVDGFKTLEEG
                     QKVTFDIQDGQRGPQATNINLVK"
     gene            6937..8910
                     /gene="mutL"
                     /locus_tag="VBC94_001235"
     CDS             6937..8910
                     /gene="mutL"
                     /locus_tag="VBC94_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEO6979510.1"
                     /translation="MSKIIELPDILANQIAAGEVVERPSSVVKELVENAIDAGSSQIT
                     IEVEESGLKKIQITDNGEGMTSEDAVLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     IASISLMTIKTATEQGKQGTLLVAKGGNIEKQEVVSSPRGTKILVENLFFNTPARLKY
                     MKSLQSELAHIIDIVNRLSLAHPEVAFTLINDGKEMTKTSGTGDLRQAIAGIYGLNTA
                     KKMIEISNADLDFEISGYVSLPELTRANRNYITLLINGRYIKNFLLNRSILDGYGSKL
                     MVGRFPIAVIDIQIDPYLADVNVHPTKQEVRISKERELMSLISSAISESLKQYDLIPD
                     ALENLAKTSTRSVDKPIQTSFSLKQSGLYYDRTKNDFFIDADTVSEPIANFTNLDKSD
                     GSVDNDVKNSVNQGATQSPNIKYASRDQADSENFIHSQDYLSSKQSLNKLVEKLDSEE
                     SSTFPELEFFGQMHGTYLFAQGNGGLYIIDQHAAQERVKYEYYREKIGEVDNSLQQLL
                     VPFLFEFSSSDFLQLQEKMSLLQDVGIFLEPYGNNTFILREHPIWMKEEEVESGVYEM
                     CDMLLLTNEVSVKKYRAELAIMMSCKRSIKANHTLDDYSARHLLDQLAQCKNPYNCPH
                     GRPVLVNFTKADMEKMFKRIQENHTSLRDLGKY"
     gene            8942..10174
                     /locus_tag="VBC94_001236"
     CDS             8942..10174
                     /locus_tag="VBC94_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6979511.1"
                     /translation="MKEFFALPKQLQLRELLRFISITVGSAIFPFMAMYYVQYFGNLV
                     TGILIIITQLSGFVATLYGGHLSDAIGRKKVVIVGSLLATIGWAITIAANVPNHITPH
                     LTFVGILIIEIAHQFYSPAYEAMTIDLTNEQNRRFVYTIGYWLVNIAVMLGSGIAGIF
                     YDHHFFELLIVLLIISAICCLVVYFKFDETKPQEGTFKHDKGVLGTFKNYSQVLVDKA
                     FVVYTLGAIGSSVVWLQVDNYFSVNLKQNFEVVSVLGHTITGAKMLSLAVFTNTLLIV
                     LLMTTINKFIENWPLKRQLILGSLICGFGMLFNISLNTFGAILIAMTFFTFGEMIYVP
                     ASQVLRAEMMVEGKIGSYSGFLAIAQPVASVLAGAMVSLSYFTGKIGVQITLTIFMLA
                     GLVLILYATKMKNIEIGK"
     gene            10176..10766
                     /gene="ruvA"
                     /locus_tag="VBC94_001237"
     CDS             10176..10766
                     /gene="ruvA"
                     /locus_tag="VBC94_001237"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEO6979512.1"
                     /translation="MYDYIKGKLSKITAKFIVVETAGLGYVIYVANPYSFSGYVNQEV
                     TIYLHQVIRDDAHLLFGFHTENEKEIFLNLISVSGIGPTTALAIIAVDDNEGLVSAID
                     NSDIKYLTKFPKIGKKTAQQMILDLSGKFVEASGESATSRKVSSEQNSNLEEAMEALL
                     ALGYKATELKKVKAFFEGTNDTVEQYIKSSLKMLMK"
     gene            10789..11340
                     /locus_tag="VBC94_001238"
     CDS             10789..11340
                     /locus_tag="VBC94_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEO6979513.1"
                     /translation="MKRCSWVNLDNPLYVAYHDKEWGRAVHDDHVLFELLCLETYQSG
                     LSWETVLNKRQEFRQVFHHYNIEKVAAMSDADLEIILQNPRVIRHRLKLFSTRQNARS
                     IILIQKEFGSFDRSIWSFVDNKVQVNSVNNYNDVPASTTLSERLSKDLKKRGFKFVGP
                     TCLYSFIQAAGMVNDHENICDFK"
     gene            11429..12688
                     /locus_tag="VBC94_001239"
     CDS             11429..12688
                     /locus_tag="VBC94_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEO6979514.1"
                     /translation="MRAEIIAVGTEILTGQIVNTNAQFLSEKMAELGIDIYFQTAVGD
                     NEERLLSLLDIASQRSQMVILCGGLGPTEDDLTKQTLAKFLGKSLTVDLLASQKLDRF
                     FASRPQFARTPNNERQAQLVEGSIPLQNLTGLAVGGIVTSKGVQYMVLPGPPSELKPM
                     VMEQVVPILSNNGTKLYSRVLRFFGIGESQLVTILEDIIKNQTDPTIAPYAKIGEVTL
                     RLSTKAENQNEADFKLDSLEKEILALKTLDNRKLKDLLYGYGDNNSMARTVLELLKVQ
                     NKTITAAESLTAGLFQSQLAEFSGASQVFNGGFTTYSMEAKSQLLGIPKKKLQEYGVV
                     SHFTAEAMAQQARQLLKADFGIGLTGVAGPDELEGYPAGTVFIGIATPEGVSSIKVSI
                     GGKSRSDVRHISTLHAFDLVRRALLKI"
     gene            12762..13901
                     /gene="recA"
                     /locus_tag="VBC94_001240"
     CDS             12762..13901
                     /gene="recA"
                     /locus_tag="VBC94_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEO6979515.1"
                     /translation="MAKKTKKAEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIVEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYSSVRLDVRGNTQIKGTGEHKDHNVGKETKIKVVKNKVAPPFREAF
                     VEIMYGEGISRTGELIKIASDLDIIQKAGAWYSYNGEKIGQGSENAKKYLADNPAIFD
                     EIDHKVRVHFGMTEDDSPVQSELVEEKNEADDLVLDLDNAIEIEE"
BASE COUNT         4526 a         2202 c         2867 g         4418 t
ORIGIN      
        1 caaaagcctt tgtttccgtt gcagaaaatg ttcatattat tggacgattc gttgatgatg
       61 attaactgta tatatgactt ttggtcaacc gccactaagg aaacattatg atgttattaa
      121 gctaagttgt ttaaacaact ttagcttttt tgaataagga aggattaagc ctatgttgaa
      181 actggattta aaaacaaaga taaaagaagc tattttaatt gcctttggtg tggctctcta
      241 tactttcggc tttgtcaaat ttaatatggc taatcatttg gctgaaggag gtatttccgg
      301 tgtcactttg attatccatg cacttttcgg agttaatcca gcactttcat cacttctcct
      361 taatatcccg ttatttatat taggggctag gattttaggg aaaaaatcct tattattaac
      421 tatatatggt acagtgttaa tgtctttttt catgtggttt tggcaacaaa tccctgtaac
      481 agtccctttg aaaaacgata tgatgttagt tgcagtagct gctggaattt tagcaggaac
      541 tggtagtggt ttagtattta gatatggtgc aactacaggt ggtgcagata ttattggtcg
      601 tattgtagag gaaaaatctg gaataaaatt agggcaaacc ttattgttta ttgatgctat
      661 agttctaacg tcttcacttg tttatattaa tttacaacag atgctatata cgttagtagc
      721 tagctttgta tttagtcaag tgttaacaaa tgttgaaaat gggggctaca ctgttcgtgg
      781 aatgatcatt attaccaaag aatcagaatc tgcagcagca accattttgc atgaaattaa
      841 taggggtgta acttttttac ggggccaagg tgcctactca ggtagagagc atgatgtctt
      901 gtacgtagct cttaacccaa gtgaagtgcg tgatgtgaaa gaaatcatgg ctgatttaga
      961 cccagatgct tttatttctg ttataaatgt agatgaagtt attagctcag attttaaaat
     1021 tcgacgaaga aattacgata aataaaaata caggctgtct ggcctgtatt tttattttgg
     1081 tcctccactt aagaaactac caagcataat agctgagata gcagttccct tgtaatggct
     1141 agcttttgtt tttaccaagc gactaagatc aaatatgctt ttaggagcat caaattgatg
     1201 tgttaagacg tattgattga taagtgagtc aatttttgta gctaggagtg agttcatccc
     1261 ctctttttca agttgactag cgagctcgaa aatagcttga cgaaaataat catctgcttg
     1321 tattacttct tcgctataaa ctttactaag agcatctaat atttcttttt ctgatggcat
     1381 ggcattctcc tgtaacttat tgtgctatta ttataatata aaaaattaag actgtcataa
     1441 atttgacagt cttagtaatg tcatttctta catttcattc ggagcatcaa ctcctaggag
     1501 gcgaagagat tcttttaata ctgtagcagt tgcatagcaa agtgctagac gactactgat
     1561 ttcagcatct tcatctaaaa tacgtgtgtg ggcgtaatat ttattgaagc actgagctaa
     1621 attgatagcg aattttgcaa taatagaggg ctcaaaatta tctgctgcac gcacaataat
     1681 acgaggaaaa tcttggatga gtttgatgat ttcccagctt tcaacatcgt tgagactgta
     1741 attatcgcta ttagatgggt taaagttagc tttacgtaag attgattgaa tacgagcatg
     1801 tgcatattgt acatatggcc cagtttcacc ttcaaatgat accattgctt ctaagtcaaa
     1861 gtcataacca tttgtacgat cagttttcaa atcgtaaaat ttaatagcac ctacaccaac
     1921 tgcttgagca actttatctt tatctgctag gttagggttt ttagcttcaa tctgagatgc
     1981 cgcacgatta atagcttctg caacagtagg ttcaaggaga attacatttc ctttacgagt
     2041 tgataatttt gctcctcctt ttgttacaag gccaaaagga acatgagtca tatcatcgga
     2101 ccaatcgtag tccatttctt taagaacagc ttttagttgt ttaaagtgtg cagactgttc
     2161 gtttcctaca acgtagattg attttgcaaa atcgtaggta cgtttacggt aaagtgcagc
     2221 agctaagtca cgtgtaatgt agagagttgc tccatcggat tttttaatga gggcaggatg
     2281 ttcaataccg tatttttcta ggttaacaac ttgtgcacct ttagattcga ctaggaggtt
     2341 tttagattct agcagttcaa gcacttcatc cattttgtcg ttgtagaatg cctcgccgtt
     2401 atagctatca aatgtaacat tcattttatc gtaaagacgg ttgaattcta ataatgactc
     2461 gtcacggaac cattgccata attcagttgc ttcaggatcg ttagcctcaa gtttacgaaa
     2521 ccactcacgt gcctcttcat caacgctagg gtctatctcg gcttcagcat taatacggac
     2581 ataaagtttt aaaagttcat caatgggatg agcacggaca gcctcttcat tgccccattt
     2641 tttataggca acgattaaca ttccaaattg tttaccccag tcgccaaggt gattaatttt
     2701 aacaggatga tagccaattt tatcaaaaat attagctaaa ctatcaccaa taactgttga
     2761 acgaaggtgt ccaattgaga atggcttagc aatatttgga ctagacatat caatggcaac
     2821 atttcgtcct tcaccaatat tttgagtagc ataggcagaa ccatcagata agacttgttt
     2881 taaaacttga cttgagattg cggctttatc aaggaaaaag ttaacgtaag gtccaacagc
     2941 ttccaccttt tcaaattggt cgcttttaat ttgttcagca atgtcactag caattatttg
     3001 aggtgctttg cgtagagttt tagccaaaga gaatgctggg aaagctaaat ctcccatgct
     3061 cgaatttttt ggggtttcta ataaagaaag aatggccgat tgttccatat atggaacaac
     3121 tttttgaatt tcactcgcaa ttagatgttt tgtatccatg gtatctccta ttttaattag
     3181 taatatagtc accctatttt aacataatta tgaaaaatag acgagaactt gttgattaaa
     3241 ttcataatgc attctgtaaa gaactagcct actttatagc ttttttacag aatgattacg
     3301 atatcactac taagtgaaaa agacaatttt ttttgatata atttggtaag cataattcta
     3361 actaaaaagg attgaataac atgaataaaa tagaacgcca aaaacgtatt aaacgtttaa
     3421 tccaatcagg gcagattggc acacaagaag aaattaaatt acatttaaaa aatgaaggaa
     3481 tcgatgtcac gcaagcgact ctatcaagag acttgcgtga gattggtctt ttaaaattgc
     3541 gtagcccaga aggtaagcta tattatagtt tatcaacagc tacttcaaat cgatttagcc
     3601 cagccttacg ctcctatatt ttgaaagtat cgcgagcaag ttttatgtta gttttaaata
     3661 ctaatttagg ggaggctagc gttttagcaa actttattga tgagaaaggg ctgccagaga
     3721 ttttaggaac aatggcagga gcagacacct tgttggtgat ttgtcaaaat gaagatattg
     3781 ccaaagtttt tgaaaaagaa ctatcagtag gtttataaat ataatgattg aggagctgag
     3841 catggcagcc agccccgtta gttaggagtg agaaatggcc aagccaacga tatcaccggg
     3901 aatgcaacag tatctggata taaaagagaa ttatccagat gcttttttgc tttttagaat
     3961 gggtgatttt tatgaattat tttatgatga tgcggtaaaa gcagcacaaa tcctggaaat
     4021 tagcttgact agtcgaaata agaacgcaga aaagccaatc ccaatggcag gagttcccta
     4081 tcactcagct caacagtata ttgacgtttt agttgaatta ggttacaaag tagccattgc
     4141 tgagcagatg gaagatccta aaaaagctgt gggagtggtc aagcgtgagg tagtgcaagt
     4201 tgttacccca ggaacggttg tggagtcaac gaagccggat agtgctaata atttcttagt
     4261 agcgattgat tcgcaagatc aacaaacatt tggtctagca tatatggatg tctcaactgg
     4321 agagtttcag gcaacccttt taacagattt tgagtccgtc cgtagtgaaa tactaaattt
     4381 aaaagctcgt gagattgtag taggatatca attgacggac gaaaaaaatc acctactgac
     4441 gaagcagatg aacttgcttt tatcatacga agacgaacga cttaatgaca ttcatttgat
     4501 tgatgagcag ttaactgatt tggaaatatc tgctgcggaa aaacttttac aatatgtgca
     4561 tagaacacaa aagcgtgaac ttagtcattt acagaaagta gttcattatg aaataaagga
     4621 ctatttacaa atgtcatatg caacgaaaaa tagtctagat ttactggaga atgctagaac
     4681 aagcaagaag cacggaagtc tttactggtt gttagatgag actaaaacag cgatgggaac
     4741 tcgaatgctg agaacttgga ttgacaggcc tttggtaagt atgaatcgaa tcaaggaacg
     4801 tcaagatatt attcaagtgt ttcttgatta tttttttgag agaaacgatc tcacagaaag
     4861 tttaaagggt gtatatgata ttgaacgcct agcaagtcga gtatctttcg gaaaagccaa
     4921 ccctaaagat ctattgcaac tcggacagac cttatcacaa attcctcgga ttaaaatgat
     4981 tttacagtcc ttcaatcaac ctgagcttga catcattgtc aacaaaattg acactatgcc
     5041 tgaattagaa agtttaatta atacggcgat agccccagaa gcacaggcta ctatcactga
     5101 gggaaacatt atcaagtctg gatttgataa gcaattggat aattatcgaa cagtgatgcg
     5161 tgaaggtaca ggttggattg ctgatattga agctaaggaa agagcagcaa gtggtatcgg
     5221 tacccttaaa attgattata ataaaaaaga cggctattac ttccatgtga ccaattccaa
     5281 tttatcacta gtaccggagc attttttccg taaagcgaca ttaaaaaatt ctgaacgcta
     5341 tggaacagca gaactagcca aaattgaagg tgaaatgctc gaagctcgcg agcaatcttc
     5401 aaatttagaa tatgatattt ttatgcgtgt tcgtgctcaa gtagaatctt atattaaacg
     5461 tcttcaagag ttagcaaaga ctattgcaac cgttgatgtt ctacagagtt tggcagtagt
     5521 tgcagaaaat catcactatg ttcgtcccaa atttaatgat gaacatcaga ttaagattaa
     5581 gaatgggcgt catgcaaccg ttgaaaaagt gatgggagtg caagaatata ttcccaatag
     5641 tatctatttt gatagtcaga cagatatcca gttgattaca ggaccaaata tgagtggtaa
     5701 gtcgacctat atgcgccagt tagctctgac agttattatg gcacaaatgg gaggttttgt
     5761 atcggcagac gaagttgatt tgcctgtatt tgatgcaata tttactagga ttggtgctgc
     5821 tgatgactta atttctgggc aatcaacctt tatggtagaa atgatggaag cgaatcaagc
     5881 tgtaaaacga gccagtgata aatctttgat tctttttgat gaattaggtc gagggacagc
     5941 cacttatgat ggtatggcat tagctcaatc gattatagaa tatattcatg accgtgttag
     6001 ggcaaaaaca atgtttgcaa ctcattacca tgagttgaca gatttatctg aacaattgac
     6061 aaggcttgtc aatgtacacg tggctacttt agagagagat ggagaagtta ccttcttaca
     6121 taaaattgaa cctggacctg cggacaagtc ttatgggata cacgtcgcaa aaatagctgg
     6181 tttaccaatt gacttattgg atagggcaac tgatatttta tcacagttgg aagctgatgc
     6241 agtacagttg atcgtatcag ccccccaaga agctgttact gctgacttaa atgaggaact
     6301 agatcctggg aagcaacaag gacaattatc gctttttgaa gaacattcaa atgcaggtag
     6361 ggttattgag gagttagaag cgatagatat aatgaatcta actccaatgc aagctatgaa
     6421 tgctatattt gatttaaaga aattattata aaatagcctt taggcaacaa aaaagagact
     6481 aaataattca tttaggctct tttttaatta tttaacaaga ttgatatttg ttgcttgtgg
     6541 tccacgttga ccatcttgaa tatcaaatgt tactttttgt ccttcttcaa gagttttgaa
     6601 gccatcaact ttaatctctg aaaaatgagc gaagacgtct gttccagttt cgctagaaat
     6661 aaagccaaaa cctttttctg agttaaacca tttcactgta ccttgtgtca tttgtttatt
     6721 acctttctaa aaatgttact gtaaaaaata aaaaaggtaa aaatgttaaa caaaaataca
     6781 acttcaaaac ttattacgct ccctaagtct aacaggtaat aggagtgatg tcaactaaaa
     6841 attgtgttat tgtcaaatta atattttgaa tatggtataa tggctaagat atcacaaaaa
     6901 aagttacaaa aaatcataaa aaaaggaggg atgaacttgt ctaaaattat tgaattaccg
     6961 gatatattag ctaaccagat tgctgctggt gaggttgttg aaaggcctag tagcgtcgtt
     7021 aaagaattag ttgaaaatgc catagatgca ggaagtagcc aaattaccat agaggttgaa
     7081 gaatctggtc ttaaaaagat tcaaataacg gataacggtg aagggatgac tagtgaggat
     7141 gccgttctca gtttacgacg tcacgctaca tccaaaatca aaagccagag tgaccttttt
     7201 cgtattcgga ccttaggttt tcgtggtgaa gctctaccat caatcgcttc gattagttta
     7261 atgacaataa aaacagctac agagcaaggg aaacaaggga cgttacttgt tgctaaaggg
     7321 ggaaacattg aaaaacagga agttgtttca agtcctcgtg gaacaaaaat tttagttgaa
     7381 aatttattct ttaatacgcc tgccagattg aaatacatga agagtttaca gtctgaacta
     7441 gctcatatta ttgatattgt taatagactc agtctagcgc atccggaagt tgcttttaca
     7501 ctgataaatg atggtaaaga aatgactaaa actagtggga ctggcgattt gagacaggcg
     7561 attgcaggta tttatggttt aaatactgca aaaaaaatga ttgagatttc aaatgcagat
     7621 ttagattttg aaatttcggg ttatgtaagt ttgcctgaat tgacacgagc taaccgaaac
     7681 tatattaccc tattaattaa cggtcgttac attaaaaatt ttcttttaaa ccgctcaatt
     7741 ctagatggtt atggttcaaa gttgatggtt ggaagatttc caattgctgt cattgatatt
     7801 cagatagatc cttatctagc agatgtcaac gttcatccga ctaagcaaga ggtgagaatt
     7861 tctaaggaac gagaattaat gagtttaata agctcagcaa tttctgagag tctgaaacaa
     7921 tatgatttga ttccggatgc tttagagaat ttagcgaaaa ctagtacccg aagtgtagat
     7981 aagcctatac aaacaagctt ctcactaaaa cagtctggtt tgtattacga taggactaaa
     8041 aatgactttt ttatagatgc agatactgta tctgaaccta ttgctaactt tacaaacctt
     8101 gacaaaagtg acggttcagt tgacaatgat gtaaaaaact ctgttaatca aggagcaacg
     8161 cagtctccta atataaagta tgctagtaga gatcaagctg atagtgagaa cttcattcat
     8221 tctcaggact accttagtag taaacagtca cttaacaaac ttgttgaaaa attagattcg
     8281 gaagaaagtt cgacttttcc agagctcgaa ttttttggtc aaatgcatgg gacctactta
     8341 tttgctcaag ggaatggtgg gttgtatatt attgatcaac atgcagctca agagcgtgta
     8401 aaatacgagt attaccgtga aaagataggt gaggttgata atagtcttca acaattgtta
     8461 gtaccatttc tatttgaatt ttcaagttca gattttcttc aattacaaga aaaaatgtca
     8521 ttattacaag atgttggtat tttcttggaa ccgtatggta ataatacttt cattttaagg
     8581 gaacatccaa tttggatgaa agaagaagag gtagagtctg gcgtttatga aatgtgtgat
     8641 atgttgttgc tcactaatga agtatcagtg aaaaaatatc gcgcagagtt ggctattatg
     8701 atgtcttgca agcgatctat taaagcaaac catacgctag acgattactc agcacgtcat
     8761 ttattagatc agttagcgca atgtaaaaat ccttataatt gccctcatgg aagacctgtt
     8821 ttagttaatt ttacaaaagc tgatatggag aaaatgttta aacgaatcca agaaaatcat
     8881 acaagcctca gggatttagg aaaatattga taattacttg ttagggaaga agggagaaaa
     8941 agtgaaagag ttttttgctt tgccaaaaca gctacagtta agagaattat tgcgttttat
     9001 ttccattacg gttggttcag ctatttttcc ttttatggca atgtattatg ttcaatattt
     9061 tggaaatcta gttacaggaa ttttgatcat cataactcaa ctatcagggt ttgtagcgac
     9121 cttatatgga ggccatttgt cagatgctat agggaggaaa aaagttgtga ttgtaggttc
     9181 actgctggca accattggtt gggctataac tatagctgca aatgttccaa atcatattac
     9241 gccacacttg acctttgtag gtatccttat cattgaaata gctcaccaat tttattcccc
     9301 tgcctatgaa gctatgacca ttgatttgac gaacgaacag aatcgtcgct ttgtttacac
     9361 tataggttat tggttggtta acatcgctgt aatgctaggt tctgggattg ctgggatttt
     9421 ctatgatcac catttttttg aattattaat agttcttctt attatttcag cgatttgttg
     9481 tttggtggtc tacttcaagt ttgatgagac aaaaccacag gagggaacct tcaagcatga
     9541 taagggagtt ttaggaactt ttaaaaatta tagtcaggtt ttagtggata aagcgtttgt
     9601 tgtttatact ttaggagcaa ttggttccag tgttgtctgg ctccaggttg ataattattt
     9661 ctcggtcaat cttaagcaaa attttgaagt agtgtctgtg ttaggacata caatcacagg
     9721 tgctaaaatg cttagtttag cagtctttac gaacactcta ttaattgtct tactaatgac
     9781 aactataaat aaattcatag agaattggcc tttgaaacgt caactaatat taggaagctt
     9841 gatttgtggt tttggaatgc tttttaatat ttctctaaac acttttggag ctattttgat
     9901 tgccatgacg tttttcacct ttggcgaaat gatttatgtt ccagctagtc aggttctaag
     9961 ggcggagatg atggtggagg gaaaaattgg atcatattct ggatttttag ctatcgcaca
    10021 acctgttgca agtgtattag caggtgccat ggtttcgctc agctatttta caggaaaaat
    10081 aggtgttcaa ataacattga ccatttttat gttagcaggt ctggttttaa tcttatacgc
    10141 taccaaaatg aaaaatattg agataggaaa atagaatgta cgattatatt aaaggaaaat
    10201 tgagtaaaat aacggctaaa tttattgttg tggaaacagc tggattggga tacgtgatat
    10261 atgtggcaaa tccctactct ttttcaggtt acgttaatca agaagttacc atctacttac
    10321 atcaagtcat tcgcgatgat gcacatcttt tatttggttt tcatacggaa aatgaaaaag
    10381 aaatttttct taatttaatt tcagtatcag gaattggtcc aacgaccgct ttagcaatta
    10441 ttgcagtcga tgacaatgaa ggtctcgtaa gtgccattga taatagtgat attaagtatt
    10501 tgactaaatt tccaaagata ggaaaaaaaa cagcacaaca gatgatcctt gatttgtcag
    10561 ggaagtttgt agaagcttct ggtgagagtg cgactagtcg taaggttagc tctgagcaga
    10621 atagcaatct tgaagaagct atggaagctt tgttagcgtt ggggtataag gcgactgaat
    10681 tgaaaaaagt aaaagccttt tttgaaggta cgaacgatac tgtggagcaa tacatcaaat
    10741 ctagtttgaa gatgttgatg aaataattta gaagtaatga ggtttcacat gaaacgatgt
    10801 tcttgggtga atttagataa tccattatat gtagcttacc atgataaaga gtgggggaga
    10861 gctgttcatg acgatcatgt actctttgag ttactatgtt tagaaactta tcaatcgggt
    10921 ttatcttggg aaacagtatt aaataaacgc caggagttca gacaagtttt tcatcactat
    10981 aatattgaaa aggtagctgc aatgtcagat gcggatttag agataatctt acaaaatcct
    11041 agggttattc gtcatcgtct aaaacttttc tcaacacgac aaaatgctag gtcaattata
    11101 ttaattcaga aagaattcgg aagttttgat cgctccattt ggtcttttgt agataataaa
    11161 gtacaggtta actcagttaa caactacaac gatgtgcctg caagtactac cttatccgaa
    11221 cgcttatcta aggatttaaa aaaacgcggt tttaaatttg ttggtccaac ttgcctctat
    11281 tcttttattc aggccgcggg tatggttaat gaccatgaaa atatatgtga tttcaagtaa
    11341 tatcaatgtg atatatttta agtagcactt ctgctacttt ttttgctaaa atgtagtcat
    11401 tatttcgaat agataagagg gtgaagttgt gagggctgaa attatcgctg taggaacaga
    11461 aattttaaca ggacagatcg ttaatacaaa cgcacaattt ttgtcggaga aaatggcaga
    11521 attgggaatt gatatttatt tccagacagc tgtaggagat aatgaggaac gtttattatc
    11581 gcttttagat atagctagtc agcgcagtca aatggttatc ctatgtggtg gacttggacc
    11641 tacagaggat gatttaacga aacaaacttt ggctaaattt ttaggtaaat cattaacagt
    11701 ggatttatta gctagtcaaa aattggatcg tttttttgca agtcgtcctc agtttgctag
    11761 aacgccaaat aatgaacgac aagctcaatt ggttgaaggc tcaatacctc tacaaaacct
    11821 tactggtcta gctgttggtg gtatcgttac atctaaggga gtccaatata tggttttacc
    11881 aggtccgcct agtgaattaa agccaatggt tatggagcag gtggtaccta ttttgtcaaa
    11941 taatggcaca aaattatatt cacgtgtatt gagattcttt ggtatcggtg agagtcagtt
    12001 agtcacaatc ttagaagaca taattaagaa tcagacagat ccaactatag caccatatgc
    12061 aaagattggt gaggtaactt taaggttatc aacgaaagct gaaaatcaga atgaagccga
    12121 ctttaaatta gatagcttag aaaaagaaat acttgctctt aagacattag ataaccgtaa
    12181 attaaaagat ttattatatg gctatggtga taataatagt atggcacgta ctgttttaga
    12241 attattgaaa gtacaaaata aaacgattac tgccgcagag agcttaacag cagggttatt
    12301 tcaatctcag ttagcagaat tttctggtgc ttcgcaagtt tttaatggtg gctttacgac
    12361 ctatagtatg gaagcaaaat cacaacttct cggcatccct aagaaaaaat tgcaagaata
    12421 tggagttgtc agtcatttta cagctgaggc aatggctcag caagctcgcc agcttttaaa
    12481 agcagatttt ggtatcggtt taacgggagt tgctggtcct gatgagttgg aaggataccc
    12541 tgcaggtaca gtttttattg ggattgcaac ccctgaaggc gtttcttcta ttaaagtatc
    12601 gattggaggg aagagccgct cagatgtccg tcatatcagt actttacatg cttttgacct
    12661 ggtgcgtagg gctttattaa agatataata tttgttataa ttagtagaac gatttctaaa
    12721 tttgaatcaa cctagattca tagaaatata aggagaagac attggctaaa aaaacgaaaa
    12781 aagcagaaga aattactaag aaattcggtg atgaacgtcg aaaagcctta gatgatgctt
    12841 tgaaaaatat tgaaaaagat tttggtaagg gcgcagtgat gcgcttaggt gagcgagctg
    12901 aacaaaaggt tcaagtaatg agttcaggtt ctcttgcttt agatatcgct ttaggagcag
    12961 gtggatatcc taaaggacgt atcgtcgaaa tttatggacc agagtcatct ggtaaaacaa
    13021 cggttgccct tcatgccgta gctcaagccc aaaaggaagg tgggattgct gcctttattg
    13081 atgctgagca cgctcttgac ccagcctatg ctgcggctct tggagttaat attgatgagt
    13141 tactcttgtc tcaacctgac tcaggtgagc aaggtcttga aattgcaggt aaattgattg
    13201 attcaggcgc agttgattta gttgttgtcg attcagttgc agcccttgtt cctcgtgctg
    13261 agattgatgg tgatattggt gacagccacg ttggtttgca agctcgtatg atgagtcaag
    13321 caatgcgtaa attgtcagca tccattaaca agacaaagac aatcgcaatt tttatcaacc
    13381 agctaaggga aaaagtaggc gttatgtttg gtaaccctga aacaacacct ggtggtcgtg
    13441 ctttgaaatt ttattcatcg gttcgtctag atgttcgtgg taatactcaa atcaagggga
    13501 ctggtgagca taaagatcat aatgttggta aagagacaaa aatcaaagtt gttaaaaata
    13561 aagttgctcc accattccgt gaagcttttg tcgaaattat gtatggtgaa ggtatttctc
    13621 gcacgggtga gttaattaaa attgctagtg atttggatat tatccaaaaa gcaggtgcat
    13681 ggtactcata taatggtgag aaaattggac aaggttctga aaatgccaaa aaatatttag
    13741 cagataatcc agcaattttt gatgaaattg accataaagt gcgcgtgcac tttggaatga
    13801 ctgaagatga ttcgccagtt cagtctgagc tcgttgaaga aaaaaatgaa gctgatgatc
    13861 ttgttttaga tttggacaat gctattgaaa ttgaggaata aaatagcaac tatacatctt
    13921 gaacttgaat aaatgtaact aaaataagtt ctgacagtgg ttggaactta tttttatact
    13981 aaaaataagc ttaaaaatcc ctaaaatcta ttt
//
LOCUS       DATROQ010000046        14010 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.047, whole genome shotgun sequence.
ACCESSION   DATROQ010000046 DATROQ010000000
VERSION     DATROQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14010
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.047"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            56..826
                     /gene="sufC"
                     /locus_tag="VBC94_001241"
     CDS             56..826
                     /gene="sufC"
                     /locus_tag="VBC94_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEO6979516.1"
                     /translation="MSVLEIKNLHVSIEDKEILKGLNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTAGEILFDGEDILELEVDERARLGLFLAMQYPSEVPGITNAEFIR
                     AAMNAGKADDDKISIRQFITKLDEKMELLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGEGFGAMIITHYQRLLNYITPDKVHV
                     MMDGKVVLSGGPELAVRLEKEGYAQIAEELGLEYKEEV"
     gene            863..2125
                     /gene="sufD"
                     /locus_tag="VBC94_001242"
     CDS             863..2125
                     /gene="sufD"
                     /locus_tag="VBC94_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEO6979517.1"
                     /translation="MSKEAILNFLQAKGEPTWLQELRLKAFEKIEELELPVIERVKFH
                     RWNLGDGTILENDYTANIPDFTELGNNPKLVQIGTQTVLEQVPMELIEKGVVFTDFYS
                     ALEEIPEVIERYFGKARPFEEDRLAAYHTAYFNSGAVLYIPDNVEITQPIEGLFYQDS
                     QSKVPFNKHILLIVGKNAKVSYLERFESIGDGTERTSANISVEVIAQAGSQIKFASID
                     RLGENVTTFISRRGRHSSDATIDWALGVMNEGNVVADFDSDLIGDGSHANLKVVAASS
                     GRQVQGIDTRVTNYGCNSVGHILQHGVILERGTLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDKARSDANPILLIDENDVTAGHAASIGQVDPEDLYYLMSRGLNQKTAEQLVIRGF
                     LGTVIAEIPVKEVRDEMIAVIDTKLEKR"
     gene            2127..3359
                     /locus_tag="VBC94_001243"
     CDS             2127..3359
                     /locus_tag="VBC94_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6979518.1"
                     /translation="MTLLDSYKLKQDFPILNQLVNDEPLIYLDNAATTQKPNQVLETL
                     RDYYQNDNANVHRGVHTLAERATAQYETAREKARQFLNAKSSKEILFTRGTTTGLNWV
                     AKFAESILERGDEVLISIMEHHSNIIPWQQACERTGAKLVYAYLKDGSLDLEDFYNKL
                     SSKTKFVSLAHISNVLGCITPVKAIAERVHQVGAYMVVDGAQSAPHMAIDVQDLDCDF
                     FALSGHKMLGPTGIGVLYGKESILDKMPPVEFGGEMIDFVYEHSATWKELPWKFEAGT
                     PNIAGAIAFGEALDYLTDVGMDEIHQYEQSLVSYVLPKLQAIDGLTIYGPSDAESHVG
                     VIAFNLEGLHPHDVATAMDYEGVAVRAGHHCAQPLINHLGIHSAVRASFYFYNTKEDC
                     DKLVDAIQKTKEFFNGTL"
     gene            3346..3789
                     /locus_tag="VBC94_001244"
     CDS             3346..3789
                     /locus_tag="VBC94_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEO6979519.1"
                     /translation="MALSKLDNLYMAVVADHSKHPHHHGFLEGVEQVQLNNPTCGDVI
                     SLSVKFDGNIISDIAFAGNGCTISTASSSMMTDAVIGKTKEEALQLADVFSKMVQGNQ
                     NPKQEKLGDAEFLAGVSKFPQRIKCATLSWNALRKAIERDNQAEL"
     gene            3889..5307
                     /gene="sufB"
                     /locus_tag="VBC94_001245"
     CDS             3889..5307
                     /gene="sufB"
                     /locus_tag="VBC94_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEO6979520.1"
                     /translation="MSEINEKVEPQPIDLGEYKFGFHDDVKPIYSTGKGLNEAVIREL
                     SAAKGEPEWMLDFRLKSLETFNKMPMQTWGADLSDIDFDDIIYYQKASDKPARDWDDV
                     PEKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEYDKLGIVFTDTDSALKEY
                     PELFKKYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRATAKKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANKGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKDSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSEAEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(5378..6565)
                     /locus_tag="VBC94_001246"
     CDS             complement(5378..6565)
                     /locus_tag="VBC94_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO6979521.1"
                     /translation="MPKLIVSFLCILLSLTCVNSVQAEEHKDIMQITREAGYDVKDIN
                     KPKASIVIDNKGHILWEDNADLERDPASMSKMFTLYLLFEDLAKGKTNLNTTVTATET
                     DQAISKIYEISNNNIHAGVAYPIRELITMTAVPSSNVATIMIANHLSQNNPDAFIKRI
                     NETAKKLGMTKTHFYNPSGAVASAFNGLYSPKEYDNNATNVTTARDLSILTYHFLKKY
                     PDILNYTKYPEVKAMVGTPYEETFTTYNYSTPGAKFGLEGVDGLKTGSSPSAAFNALV
                     TAKRQNTRLITVVLGVGDWSDQDGEYYRHPFVNALVEKGFKDAKNISSKTPVLKAVKP
                     KKEVTKTKTKSIQEQPQTKEQWWTKTDQFIQSHFVSILIVLGTIASLCLLAGIVLLIK
                     RSR"
     gene            complement(6718..7953)
                     /locus_tag="VBC94_001247"
     CDS             complement(6718..7953)
                     /locus_tag="VBC94_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO6979522.1"
                     /translation="MKKIITSILLLSCIFFMPTISAESFNASAKHALAVDLDSGKILY
                     EKDANKPAAIASLTKIMTVYMVYKEIDNGNLKWNTKVNISDYPYQLTRESDASNVPLE
                     KRRYTVKQLVDAAMISSANSAAIALAEHISGTESKFVDKMTAQLEKWGIHDSHLVNAS
                     GLNNSMLGNHIYPKSSQNDENKMSARDIAIAAYHLVNEYPSILKITSKSVAKFDKDIM
                     HSYNYMLPDMPVFRPGITGLKTGTTELAGQSFIATSTESGMRLLTVIMHADKADKDKY
                     ARFTATNSLLNYITNTYEPNLVLAKGAAYKGKEASVRDGKEQSVIAVAKNDLKVVQKK
                     NITKQNQLKINFKKELTAPITKKENLGKAYYVDLNKVGKGYLIKEPSVHLVAKDSIER
                     SFFLKVWWNHFVRYVNEKL"
     gene            8195..9853
                     /locus_tag="VBC94_001248"
     CDS             8195..9853
                     /locus_tag="VBC94_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6979523.1"
                     /translation="MAKQKNNWRRVGVGVLTLASVATLAACGSKSASQDSNGAINWAI
                     PTEINTLDLSKVTDTYSNLAIGNSSSNFLRLDKDGKTRPDLATKVDVSKDGLTYTATL
                     RKGLKWSDGSKLTAKDFVYSWQRLVDPKTASQYAYLAVEGHVLNADKINEGQEKDLNK
                     LGVKAEGDDKVVITLSSPSPQFIYYLAFTNFMPQKQEVVEKYGKDYATTSKNTVYSGP
                     YTVEGWNGSNGTFTLKKNKNYWDAKNVKTKEVRIQTVKKPDTAVQMYKRGELDAANIS
                     NTSAIYQANKNNKDVTDVLEATTAYMEYNTTGSVKGLDNVKIRRALNLATNRKGVVQA
                     AVDTGSKPAIAFAPTGLAKTPDGTDLAKYVAPGYEYNKTEAAKLFKEGLAESGLTKLK
                     LTITADADAPAAKNSVDYIKSTWEAALPGLTVEEKFVTFKQRLEDSRKQNFDIVVSLW
                     GGDYPEGSTFYGLFKSDSQNNDGKFANKDYDAAYNKAISEDAMKPAESAKDYKEAEKI
                     LFEQGAYNPLYFRSGKGLQNPKLKGVIRNTTGLSIDFTHAYKNN"
     gene            9968..10882
                     /locus_tag="VBC94_001249"
     CDS             9968..10882
                     /locus_tag="VBC94_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6979524.1"
                     /translation="MIKYILKRVAILLVTLWVVITLSFFLMQILPGTPYNNPKLTEEM
                     ITLLNKQYGLDKPVWQQYLTYLWNVLHGDFGTSYQSVNQPVSRMISLRLGVSVHLGVQ
                     ALVFGVLGGILVGAISARHKNDKVDGILSVIATLGISMPSFIIGILLLDYFGFKWNLL
                     PLSGWGTFSQTILPSLALGLPTLASVSRFFRSEMIETLNSDYVQLARSKGMTIRQVTR
                     KHAYRNSMIPILTLIGPLAAGLLTGSALIEQIFSIPGIGQQFVTSIPTKDYPVIMGTT
                     IVYAVMLMVAILITDVVISIVDPRVRLQ"
     gene            10892..11923
                     /locus_tag="VBC94_001250"
     CDS             10892..11923
                     /locus_tag="VBC94_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6979525.1"
                     /translation="MADKNRTFKLVGAGSSSTQEKIEKPALSFMQDAWRRLKKNKLAV
                     VSLYLLALLLTFSLASNLFVTQKDANGFDSKKVTTYRNLPPKLSSNLPFWNGSIKYAG
                     NTESTDAYKSQNVPEKVKYALGTDSLGRSVAKRIIVGIRISLLVAIAATFIDLIIGVT
                     YGLVSGFAGGRLDTLMQRVVEVISSIPNLVIVTMLGLVLGNGITAIIISIAFTGWTSM
                     SRQVRNLTLSYREREFVLAARSLGESPIKIAFKHILPNISGIIIVQIMMTIPSAIMYE
                     AVLSAINLGVKPPTASLGSLISDAQENLQYYPYQVILPALALVMISLAFILLGDGLRD
                     AFDPKSSDE"
     gene            11936..12985
                     /locus_tag="VBC94_001251"
     CDS             11936..12985
                     /locus_tag="VBC94_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979526.1"
                     /translation="MEKETILSVNNLHVDFHTYAGEVKAIRDVNFELKKGETLAIVGE
                     SGSGKSVTTRTLIGLNAKNSEISGNVQFKGRNLVELSEEEWTKVRGNEISMIFQDPMT
                     SLDPTMKIGMQIAEPMMIHQKISKKDALKLALELMKDVGIPNAEEHINDYPHQWSGGM
                     RQRAVIAIALAADPEILIADEPTTALDVTIQAQILNLMKKIQAERDSSIVFITHDLGV
                     VAGMADRVAVMYAGKIVEFGTVDEVFYNPQHPYTWGLLNSMPTTETESGSLESIPGTP
                     PDLLNPPKGDAFAARNEFALDIDHEEEPPYFKVSETHFAATWLLDERSPKVLPPLPIQ
                     KRWEKWNEIEGRKAQ"
     gene            12982..13914
                     /locus_tag="VBC94_001252"
     CDS             12982..13914
                     /locus_tag="VBC94_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6979527.1"
                     /translation="MTENRKKLVEVKNVSLTFNKGKANEVRAIDNVSFDIYEGEVFGL
                     VGESGSGKTTVGRSILKLYDISDGEITFNGEVISHLKGKALHSFRKDAQMIFQDPQAS
                     LNGRMKIRDIVAEGLDIHKLAKSKSDRDSKVQALLDLVGLNKDHLTRYPHEFSGGQRQ
                     RIGIARALAVEPKFIIADEPISALDVSIQAQVVNLMQKLQREQGLTYLFIAHDLSMVK
                     YISDRIGVMHWGKLLEVGTSDDVYNKPIHPYTKSLLSAIPEPDPESERQRVHQPYNPA
                     IEQDGQERQMHEITPGHFVLSTPQEAEEYKKQIL"
BASE COUNT         4508 a         2305 c         2825 g         4372 t
ORIGIN      
        1 ttaaaccata gagaaatatc acatattctc tgttaaccaa gaaaaggagt gtacaatgtc
       61 tgtacttgaa atcaaaaatc ttcatgtttc tattgaagat aaagaaattt taaaaggtct
      121 taatttaacg ctgaaaacag gagaaattgc tgctatcatg ggtccaaatg gtactgggaa
      181 gtcgacactt tcagcagcta ttatgggaaa tcctaattat gaagttacag caggtgaaat
      241 tctttttgat ggtgaagata ttttagagct agaagtggat gaacgtgctc gtttgggtct
      301 atttcttgcg atgcaatacc cttcagaggt tccagggatt actaatgctg aatttatccg
      361 agcagctatg aatgcgggaa aggctgatga tgataaaatt tccatccgcc aatttattac
      421 taagttagat gaaaagatgg aactacttgg catgaaagaa gaaatggcgg aacgttatct
      481 taacgaaggt ttttcaggtg gtgagaagaa gcgcaatgaa attttacaac tcctaatgtt
      541 agagcctaag tttgccctct tggatgagat tgactctggc ctggatattg atgctcttaa
      601 agtggtatca aagggagtca atgaaatgcg tggtgaaggt tttggtgcta tgattattac
      661 acactatcaa cgccttttga attacattac accagataaa gtacacgtta tgatggacgg
      721 gaaggtagta ttgtcaggag gaccagaatt agcggttcgc cttgagaaag aaggttatgc
      781 tcaaatcgct gaagagcttg gtttagagta taaagaagaa gtctaatttg tcacttcaac
      841 tagaagagaa aaggagttac aaatgtctaa agaagctata ttaaattttt tacaagccaa
      901 aggtgagcct acttggttac aagaattacg actaaaggct tttgaaaaaa tagaagaact
      961 agaattacca gtaattgaac gtgtaaaatt tcatagatgg aatttaggtg atggaactat
     1021 tttagagaat gactatacag ctaacattcc agatttcaca gagttaggaa acaatcccaa
     1081 gttggttcaa attgggaccc aaactgtcct tgaacaagtt cctatggaat taatagagaa
     1141 aggcgttgtc tttacagatt tctattcggc tttggaagaa attccagaag tcatagagcg
     1201 ctactttggg aaagcacgtc cttttgaaga agatagactt gctgcctatc acacagctta
     1261 cttcaacagt ggtgcagtgc tttatattcc agataatgtt gaaattactc aaccaatcga
     1321 aggtcttttt tatcaagata gtcaatctaa agtaccattt aataagcata tccttcttat
     1381 tgttggaaaa aatgcaaaag ttagttactt agagcgattt gagtcaattg gtgatgggac
     1441 agaacgaaca agtgctaata tttctgtaga agtaattgcc caagcaggta gccaaattaa
     1501 atttgcctca attgatcgcc tcggagaaaa tgtgacaacg tttataagtc gtcgtggtcg
     1561 tcacagtagc gatgcaacaa tagattgggc ccttggggtg atgaatgagg gcaatgttgt
     1621 tgctgacttt gattctgatt taattggtga tggaagccac gctaatttaa aagtagttgc
     1681 tgcttcgtca gggcgtcaag ttcaaggaat tgatacgcgt gttacaaact acggctgtaa
     1741 ttcagtagga catattttac agcatggggt tattttagag cgtgggactc ttacttttaa
     1801 cggtatcgga catattataa aaggtgcaaa gggagcagac gcccaacaag aaagccgagt
     1861 tctcatgtta tcagacaaag cacgttcaga tgctaatcct atccttttga ttgatgaaaa
     1921 tgatgttaca gctggtcacg cagcctcaat tggtcaggtt gacccagagg atttatatta
     1981 tttgatgagc cgtggcttga accaaaaaac agcagagcaa ttagttattc gtggattttt
     2041 aggaacagtc attgctgaaa taccagtaaa agaagttcgc gatgaaatga ttgccgttat
     2101 tgatacaaaa ctagaaaaac gttaatatga cattactaga tagttataaa ctaaagcaag
     2161 atttcccaat tttaaatcaa ttagttaatg atgagccgct tatctattta gataatgcag
     2221 caacgacaca aaaaccgaat caagttttag aaactttgcg agattactac caaaatgaca
     2281 atgcaaatgt ccatcgtggt gttcatactt tagcagaacg agcgactgct cagtacgaga
     2341 ctgctcgtga aaaagcaagg cagtttttaa atgctaaatc aagtaaagaa attttattca
     2401 cacgtggcac cacaacagga ttaaattggg tagctaagtt tgccgaaagt attttagaac
     2461 gaggtgatga ggtacttatt tccattatgg agcatcattc taatattatc ccatggcaac
     2521 aagcttgcga gagaactggt gctaaattgg tttatgccta tctaaaagat ggttctttag
     2581 atttagaaga tttctataat aaattatcct ctaaaactaa atttgttagt ttggcacata
     2641 tctcaaatgt acttggctgt attacaccag taaaagcaat agcagaacgt gtgcatcaag
     2701 ttggggcata catggttgtt gatggagctc aatcagcgcc acatatggcg attgatgttc
     2761 aagaccttga ttgtgatttc ttcgccttat ctggccataa gatgcttggt cctactggta
     2821 ttggtgttct ttatgggaaa gaaagcattt tagataagat gccacctgtc gaatttggtg
     2881 gtgaaatgat tgattttgtc tatgagcata gtgcaacatg gaaggaattg ccctggaagt
     2941 ttgaagctgg aacgcctaat attgctggag cgatcgcttt tggagaagct ttagattatt
     3001 taacggatgt tggtatggat gagattcatc aatatgaaca aagtttagtt tcttatgtgt
     3061 tgcctaaatt acaggctatc gatggtttaa ctatttacgg acctagtgat gctgaatctc
     3121 atgtaggcgt tattgccttc aatttagaag gtttacaccc acatgatgtg gcaactgcaa
     3181 tggattacga aggagtggct gttcgtgcag gtcatcattg tgcacaaccc cttattaatc
     3241 atcttggtat ccattcggcg gttcgagcaa gtttctattt ctataatacc aaagaggatt
     3301 gcgacaaatt ggtagacgct atccaaaaaa ctaaggagtt tttcaatggc actctctaaa
     3361 ttagataacc tctacatggc ggtggtagct gatcattcaa aacaccctca tcatcatgga
     3421 tttttagaag gcgttgaaca agtgcagtta aacaatccta catgtggaga tgttatttcc
     3481 ttatctgtta aatttgatgg gaatattatt tcagatattg catttgctgg taatggttgt
     3541 acaatttcta cggcttcatc aagcatgatg acagatgcag ttattgggaa aacaaaagaa
     3601 gaagcattgc agcttgctga tgtattctcc aaaatggttc aaggcaatca aaatccaaaa
     3661 caagagaaac ttggggatgc agagtttcta gctggggtat caaaatttcc acaacgtatc
     3721 aaatgtgcta cgctttcgtg gaatgcttta agaaaggcta ttgagagaga taatcaggca
     3781 gaactctgac aaccatagtt gaaggaatta catttaaaca acttttactg acataaggta
     3841 gtctagtaga agaatgtctt aaatttaaat attaaagaaa ggataagtat gtccgaaata
     3901 aatgaaaaag tagaaccaca gccaatagat cttggggaat ataaatttgg atttcatgac
     3961 gatgtaaagc caatttattc tacgggaaaa ggtctaaatg aggctgttat tcgtgagtta
     4021 tctgcagcta agggtgaacc tgagtggatg ttggactttc gtctaaaatc cttggaaacg
     4081 tttaataaaa tgccgatgca gacctgggga gcagatttat cagatattga ttttgatgat
     4141 attatttatt atcaaaaagc atctgataaa cctgcgcgtg attgggatga tgttccagaa
     4201 aaaatcaaag aaacttttga aagaattggg attccagaag ctgaaagagc ctatcttgca
     4261 ggagcatcag cacaatatga atcagaagta gtttatcaca atatgaaaga agaatatgat
     4321 aagctgggta ttgtttttac ggatactgac tctgcactta aagagtaccc agagctattc
     4381 aaaaaatatt ttgctaaact tgtccctcca acagataata aattagctgc tctgaactct
     4441 gctgtatggt caggtggaac atttatttat gttcctaaag gtgttaaggt ggatattcca
     4501 cttcaaactt acttccgtat taataatgaa aatactggac aatttgaacg tactctcatt
     4561 attgttgatg agggagcaag tgttcactat gttgaaggtt gtaccgcccc aacttattct
     4621 tcaaatagtt tacatgcagc tatagttgaa atttttgcac ttgatggagc ttatatgcgc
     4681 tatacgacta ttcaaaattg gtccgataat gtctataatt tagtgacaaa acgtgctacc
     4741 gctaaaaaag atgcaacagt tgagtggata gatggaaatc taggagctaa aacaacaatg
     4801 aaatacccat cggtttacct tgatggtgaa ggagcacgtg gcacgatgtt gtctattgct
     4861 tttgcaaaca aaggacaaca ccaagatacg ggtgcaaaga tgattcataa tgccccccat
     4921 actagttcat ccattgtctc taaatcaatt gctaagggtg ggggaaaagt tgattatcga
     4981 ggtcaagtga catttaataa agattccaaa aaatcagtgt cacatataga atgtgacacc
     5041 atattgatgg atgatatttc aaaatcagat accataccgt ttaatgagat tcataattca
     5101 caggttgctt tagagcatga agcaaaggtg tctaagattt ctgaagagca actgtactac
     5161 ttgatgagtc gaggtttatc tgaagctgaa gcaacagaaa tgattgttat ggggtttgtt
     5221 gagcccttta cgaaagaatt accaatggaa tatgcggtag agttaaatcg tttaatttcc
     5281 tatgaaatgg aaggttcagt tggttaattg aagatttcaa gagataataa aaagccccta
     5341 tgttattgat gaaggggctt ttatattagc acagttatta tctagagcgc tttataagta
     5401 atactatccc agctaaaaga caaaggctag cgatggtgcc cagaacaatt aaaatagata
     5461 caaaatgtga ttggataaat tgatctgttt ttgtccacca ctgttctttt gtttgaggct
     5521 gttcttgaat agatttagtt ttggttttag taacttcttt tttaggttta acggctttta
     5581 atacaggagt tttagaagaa atatttttag cgtctttaaa acctttttct acaagagcgt
     5641 tgacaaacgg atgacgatag tactctccgt cttggtctga ccaatcgcca actcctaaaa
     5701 ccacagttat caagcgagta ttctggcgtt tagctgtaac taaggcatta aaagcagcgc
     5761 tagggctaga accagttttt aagccatcta ctccttctaa tccaaattta gcgccggggg
     5821 tagagtagtt ataagttgta aatgtttctt cataaggagt tccgaccatg gccttgactt
     5881 caggatattt tgtatagttc agtatatcag ggtatttttt aaggaaatga taggttaaaa
     5941 ttgatagatc acgtgcagtc gtaacgttag tagcattgtt atcgtattct tttggggagt
     6001 aaagtccatt aaaagcactc gctaccgccc cactggggtt ataaaagtga gtttttgtca
     6061 taccgagttt cttggcggtt tcattgattc gtttaataaa ggcgtcagga ttgttttgtg
     6121 ataagtggtt agcaatcata atagttgcta cattagatga cgggacagcc gtcatagtaa
     6181 tcagttcacg aataggataa gcaaccccag catgaatatt gttattacta atttcataaa
     6241 tcttacttat ggcttggtct gtttctgttg cagtcactgt ggtgttgagg tttgtttttc
     6301 ctttagctaa gtcttcaaat agtaaatata aagtaaacat tttagacatg ctagcgggat
     6361 cacgttctaa atcggcgtta tcttcccaca aaatatgacc tttattgtca ataacgatag
     6421 acgctttagg tttattaata tctttaacat catatccggc ttctcgggta atttgcataa
     6481 tatctttatg ttcttcagct tgcacagagt ttacacaagt cagggataat aaaatgcaga
     6541 ggaaagatac gattaattta ggcataggta actcctgaaa actttttatt aatattataa
     6601 caaaaaatca ctaataaaaa agacatttca ttagtgattt ttaattttat atattctcct
     6661 aaacctagct aaagaaactg ctgtgcagta actccttgtg tcaaagggag attactatta
     6721 aagtttttcg ttaacgtagc gcacaaaatg attccaccac actttgagga agaaactgcg
     6781 ctcaatacta tcttttgcca ctaaatgaac gctaggttcc tttatgagat agccttttcc
     6841 aaccttatta aggtcaacgt aataagcttt ccctaggttc tctttttttg taataggagc
     6901 agtaagctct tttttaaagt taatttttaa ctgattttgt ttagtgatat ttttcttctg
     6961 tactactttc aaatcgtttt tagcaacagc gatgaccgat tgttcttttc cgtctctcac
     7021 acttgcttct ttacctttat atgcagctcc tttagctaat acaaggttag gttcgtaggt
     7081 gtttgtgata tagttcaaga gagagttagt tgctgtaaag cgagcatatt tgtctttatc
     7141 ggccttatca gcatgcataa taacagtgag tagtctcatt ccactttcag tagatgtagc
     7201 aataaaagat tggccagcta attccgttgt cccagttttc aaacctgtaa tacctggtct
     7261 aaagacaggc atatctggta gcatgtagtt ataagaatgc ataatatctt tatcaaattt
     7321 agcaacggac ttactagtaa tcttaagaat ggaaggatat tcgttgacca aatggtaggc
     7381 agcaatagca atatcacgtg cactcatttt attttcgtcg ttttgtgacg attttggata
     7441 aatgtgattg cctaacatac tattatttaa gccagaagca ttgactaggt ggctatcatg
     7501 aattccccac ttttccaatt gagcagtcat tttatcaaca aatttacttt cagttcctga
     7561 aatatgttca gctaaagcaa tggctgcact gttagcacta gaaatcatgg cagcgtccac
     7621 gagttgttta acagtatagc gccttttttc taaaggaaca ttactagcat cagattcgcg
     7681 tgttagttga taagggtagt cagatatatt tactttggta ttccacttga ggttaccgtt
     7741 atcaatttct ttatagacca tataaacggt cattatttta gtcaaggaag caatagcagc
     7801 gggtttgtta gcatcttttt catacaagat ttttcctgaa tctaaatcaa ctgctaaggc
     7861 atgtttagcg gaagcattaa aagattcagc agagatggtt ggcataaaaa aaatgcaact
     7921 aagtaataga atagaagtaa taattttttt caaaataaca cctttagaaa ttttttaaac
     7981 tttgtccctc ctattatacc atatcactat gtcatactag gaattattat tttcagataa
     8041 ttgaatgaag aaaacgtttg ttataaaaat gtaaaaaaat gtaaaaaaat gaaagttaaa
     8101 aaattatctg aaaattttgc gtttttgtct tttcttaaga attttttgtg gtataatgtt
     8161 agtcactaga taaatgaatt aggagaacaa agctatggca aaacagaaaa ataactggcg
     8221 ccgtgttgga gttggtgtcc ttacacttgc ttcagttgcg actcttgctg catgtggaag
     8281 taaatcagct tcccaggatt ctaatggagc gattaattgg gctattccaa cagaaatcaa
     8341 tacactagat ttatctaaag ttacagacac ttactcaaat ctagctattg gtaactctag
     8401 tagtaatttc cttcgcttag ataaagatgg aaagacaaga ccagacttgg ctactaaagt
     8461 tgatgtttca aaagatggct taacttatac agctacatta cgtaaaggct tgaagtggtc
     8521 agatggcagt aaacttactg caaaggattt tgtttattca tggcaacgtt tagttgatcc
     8581 taaaacagct tcacaatatg cttaccttgc tgttgaaggg catgtgctta atgccgataa
     8641 aatcaacgaa ggacaagaga aagacttgaa taagctaggt gttaaggcag aaggcgatga
     8701 caaagttgtt attactttat ctagtccgtc tccgcaattc atctactacc ttgcattcac
     8761 taacttcatg ccacaaaaac aagaagttgt tgaaaaatat ggaaaagatt acgcaactac
     8821 ttcaaaaaat acagtttact caggaccata tactgttgaa ggttggaatg gttcgaatgg
     8881 tactttcacg ctgaagaaaa acaaaaatta ttgggacgct aaaaatgtaa aaacaaaaga
     8941 agttcgcatc cagactgtta aaaaaccaga taccgccgtt caaatgtata aacgtggtga
     9001 gttagatgca gctaatatct caaatacttc tgctatttat caagctaata aaaataataa
     9061 agatgtcaca gatgttctag aagcgaccac tgcctatatg gaatataata ctactggttc
     9121 tgtgaaaggg cttgataatg ttaagattcg tcgcgcctta aacttagcaa ctaaccgtaa
     9181 aggagttgtt caagcagccg ttgatacagg ctcaaaaccg gcaattgctt ttgcacctac
     9241 tggtttagcc aaaacaccag atggaactga tttggcaaaa tatgttgctc caggttatga
     9301 atataataaa actgaagcag caaaactctt taaagaaggt ttggctgaat caggcttgac
     9361 taagctaaaa ttaacaatta cagcagatgc tgatgctcct gctgccaaaa actctgttga
     9421 ctatatcaag tctacttggg aagctgctct tccaggactt actgttgaag aaaaatttgt
     9481 aacctttaaa caacgcttag aagacagtag aaaacaaaac tttgacattg tagtttctct
     9541 ttggggtggg gattatccag aaggatcaac tttctacggc ctctttaagt cggattcaca
     9601 aaataacgat ggaaaatttg ctaacaagga ctatgatgct gcttataaca aggcaatttc
     9661 tgaagatgcg atgaaaccag cagaatcagc gaaggactat aaagaagcag agaaaatttt
     9721 atttgagcaa ggtgcttata acccactcta cttccgtagt ggtaaaggtt tacaaaatcc
     9781 aaaattaaaa ggtgttattc gtaatactac aggtttgtca atagacttta cacatgctta
     9841 taaaaataat taaattactt tacactaaac ctttgcaaga attggtaatt gccagttctt
     9901 gcttttggtt aagtaaagaa agtgaaagag tttgctattt ttagcaattc taagaaaagg
     9961 aaatgatatg attaaatata ttttaaagcg tgttgctatt ttgttagtaa cgctatgggt
    10021 tgtcataacc ctatcgttct ttctgatgca gatccttcca ggaacaccct acaataatcc
    10081 aaaactcact gaagaaatga tcacattgct taataaacag tatggtttag acaagcctgt
    10141 atggcaacaa tatttgactt acctttggaa tgtacttcat ggtgattttg gaacgagtta
    10201 tcaatcagtg aatcagccag tatcacgtat gattagcttg agattaggag tttcagtaca
    10261 tctaggtgtt caggctctcg tatttggtgt tttaggcggt attttggtag gagctatttc
    10321 ggcacgtcat aagaatgata aagtcgatgg tatcttaagt gttattgcaa cgctagggat
    10381 ttctatgcct tcatttatca ttggtatctt attattagac tattttggat ttaagtggaa
    10441 cttgttgcct ttatcaggtt gggggacttt ctctcaaaca attttacctt ctttagctct
    10501 aggactccct acattagcat ctgtttcacg cttcttccgt agtgaaatga ttgaaacttt
    10561 aaattctgat tatgtgcaac tagctcgttc aaaaggtatg acaattcgtc aagtaactcg
    10621 aaaacatgct taccgtaatt ctatgattcc aattttaact ctaattggcc ctttggcggc
    10681 gggtctgtta actggttctg cactaattga gcagattttc tcgattccag gaattgggca
    10741 gcagtttgtt acttcgattc caacaaaaga ctatcctgtt attatgggga caaccattgt
    10801 ttatgcagta atgttgatgg ttgctatctt gataacagac gttgttatta gtatcgtaga
    10861 cccacgtgtt cgcttgcagt aaggagatgt tatggcagat aaaaacagaa catttaaact
    10921 tgtaggtgca ggatcttcta gcacacaaga aaaaattgaa aagcctgctc tttcgtttat
    10981 gcaagatgcg tggcgtcgct tgaaaaaaaa caaattagca gtagtttcac tctatttatt
    11041 agctctttta cttacttttt cgttagcctc aaatttattt gtaactcaga aggatgctaa
    11101 tgggtttgat tcgaaaaaag taacgacata tcgcaactta ccacctaaat tgagttcaaa
    11161 ccttcctttt tggaatggta gcattaagta cgctggaaat acagaatcta cagacgctta
    11221 taagtctcaa aatgtccctg aaaaagttaa gtatgcttta ggtacagatt ctctagggag
    11281 aagtgttgct aagcgcatca tcgtaggtat tcgaatttcc ttacttgtag ctattgcagc
    11341 tacattcatt gatttaatta tcggtgttac atacggtctt gtttcaggtt ttgctggagg
    11401 acgccttgat acattaatgc aacgtgtcgt agaggtaatt tcatcaattc caaacttagt
    11461 tattgttaca atgttaggtt tggtccttgg taacggtatt acggctatta ttatctctat
    11521 tgcttttacc ggatggactt ctatgtctcg tcaggtaagg aatttaaccc tatcatatcg
    11581 agaaagagag tttgtgcttg ctgcacgttc acttggggaa agtccaatta aaatagcctt
    11641 caaacatatt ttacctaaca tttctggtat tatcattgta caaattatga tgacaatccc
    11701 aagtgctatt atgtatgaag cggtactttc agcaatcaat ttaggtgtta aaccaccgac
    11761 agcttcatta ggatcattga tttcagatgc acaagaaaat ttacaatatt atccatatca
    11821 agttatcttg ccagctttag cattggtaat gatttcattg gcatttattt tgcttggtga
    11881 tgggttgcga gatgcttttg atccaaaatc tagcgatgaa taaggaggta taatcatgga
    11941 aaaagaaact attttaagtg ttaataatct tcatgttgac ttccacacat atgctggaga
    12001 agtaaaagca attcgtgatg tcaactttga attaaaaaaa ggtgagactc ttgcaatcgt
    12061 tggtgaatct ggttcaggaa aatctgtaac taccagaact ttaattggtt taaatgctaa
    12121 aaattcagag atatcaggga atgttcaatt taaggggcgt aaccttgttg aactatcaga
    12181 agaagagtgg actaaggtac gcgggaatga gatttctatg attttccaag accctatgac
    12241 tagtttggat ccaacgatga aaattggtat gcaaatagcg gagccaatga tgattcatca
    12301 aaaaatttca aaaaaggatg ctttaaaatt agctcttgag ctaatgaaag atgtaggtat
    12361 cccaaatgct gaagagcata ttaacgatta cccacatcag tggtctggag gaatgcgtca
    12421 acgtgcggtt atagcgattg ctttagctgc tgatcctgaa attcttattg ctgatgaacc
    12481 aacgactgct ttagatgtaa caattcaagc acaaatttta aacttaatga aaaaaattca
    12541 agcagaacga gactcctcca ttgttttcat aactcatgac ttaggggtcg ttgcagggat
    12601 ggcagaccgt gtagcagtta tgtatgcagg gaaaatcgtt gaatttggaa ctgttgatga
    12661 agtcttttat aatccacaac atccatatac ttggggatta ttgaattcaa tgccgacaac
    12721 cgaaacagaa tcaggtagtt tagagtcaat tccgggaaca ccaccagatt tgttgaaccc
    12781 acctaaagga gatgcatttg cagctcgtaa tgaatttgca ttagatattg atcatgaaga
    12841 agaaccaccg tacttcaaag tttcagaaac acatttcgct gctacatggc ttttagatga
    12901 aagatcacct aaggtgcttc ctccattacc gatccaaaaa cgttgggaaa aatggaacga
    12961 gattgaaggg aggaaagccc aatgactgaa aatcgaaaaa aattagttga agtcaaaaat
    13021 gtttctttga ccttcaataa aggaaaagct aatgaagtga gagcaattga taatgttagt
    13081 tttgacattt atgaaggtga agtgtttgga ttagttgggg aatctggatc aggaaagaca
    13141 accgttggac gttcaatttt aaaactttat gatatttctg atggcgaaat tacctttaat
    13201 ggagaagtga tctcacatct gaaaggtaaa gcattacata gttttcgtaa agacgctcag
    13261 atgatttttc aggatcctca ggctagctta aacggccgta tgaagattcg tgatatcgta
    13321 gcagaaggtt tagacataca taaattagct aaatcaaaat cagatcgtga tagtaaagtt
    13381 caggccctat tagatcttgt agggttaaat aaagatcatt taacacgtta tccgcatgaa
    13441 ttttcaggtg gacaacgaca acgtatcggg attgcacgtg ctttagcagt agagcctaaa
    13501 tttatcattg ctgatgaacc aatatctgcc ttagatgttt caatccaagc acaagttgtt
    13561 aatttaatgc aaaaattgca aagagaacaa ggattgactt atttgtttat cgcacatgat
    13621 ttgtctatgg taaaatacat ttcagatcgt atcggtgtta tgcattgggg gaaactgtta
    13681 gaagttggaa catctgatga tgtttataac aaaccaatcc acccttatac gaagagtcta
    13741 ttatcagcta ttccagaacc agatccggag agtgaacgtc aacgtgttca ccagccgtat
    13801 aatccagcta ttgagcaaga cggacaagaa cgtcaaatgc acgagattac acctggtcat
    13861 tttgttttat ctacacccca agaagctgaa gaatataaaa aacaaatatt ataaaagatg
    13921 gctagcccat cttttatttt tttactaata aaataaattt aggtatggaa ttttaaactc
    13981 tatgttattg gtgttagcca acaggaaact
//
LOCUS       DATROQ010000047        13899 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.048, whole genome shotgun sequence.
ACCESSION   DATROQ010000047 DATROQ010000000
VERSION     DATROQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13899
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.048"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..2055
                     /locus_tag="VBC94_001253"
     CDS             <1..2055
                     /locus_tag="VBC94_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO6979528.1"
                     /translation="FNSLMGNMGGYNSENKRYLINGREVTPEEFSQYRQTGKLPGQEL
                     NNQNTPTNQVSADSVLTKLGTNLTDQARQHLLDPVIGRNKEIQETAEILARRTKNNPV
                     LVGDAGVGKTAVIEGLAQAIINGDVPAAIKNKEIISIDISSLEAGTQYRGSFEENIQN
                     IIKEVKETGNIILFFDEIHQILGAGSTGGDSGSKGLADILKPALSRGELTVIGATTQD
                     EYRNTILKNAALARRFNEVKVNAPSAQDTFNILMGIRNLYEQHHNVVLPDSVLKAAVD
                     LSIQYIPQRSLPDKAIDLIDMTAAHLAAQHPVTDLKSLEKEIAEQRDKQEKAVNTEDF
                     EEALKVKTRIEELQNQIDNHTEGQKVTATINDIAMSIERLTGVPVSNMGASDIERLKE
                     LGNRLKGKVIGQNDAVEAVARAIRRNRAGFDDGNRPIGSFLFVGPTGVGKTELAKQLA
                     FDMFGSKDAIVRLDMSEYNDRTAVSKLIGATAGYVGYDDNSNTLTERIRRNPYSIVLL
                     DEIEKADPQVITLLLQVLDDGRLTDGQGNTINFKNTVIIATSNAGFGNEAFTGDSDKD
                     LKIMERISPYFRPEFLNRFNGVIEFSHLSKDDLSEIVDLMLDEVNQTIGKKGIDLVVD
                     ENVKSHLIELGYDEAMGVRPLRRVIEQEIRDRITDYYLDHTDVKHLKANLQDGQIVIS
                     ER"
     gene            complement(2148..3092)
                     /gene="mmuM"
                     /locus_tag="VBC94_001254"
     CDS             complement(2148..3092)
                     /gene="mmuM"
                     /locus_tag="VBC94_001254"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HEO6979529.1"
                     /translation="MGRFKELLESKKTLILHGALGTELESRGCDVSGKLWSAKYLIED
                     PAAIQTIHEDYIRAGADIVTTSTYQATLQGLAQVGVSESQAEDLIRLTVQLAKAAREQ
                     VWKSLTKEEKSERIYPLISGDVGPYAAFLADGSEYTGLYDIDKQGLKNFHRHRIELLL
                     DEGVDILALETIPNAQEAEALIELLAEDFPQVEAYMSFTSQDGKTISDGSAVADLAKA
                     IDVSPQVVALGINCSSPSLVADFLQAIAEQTNKPLVTYPNSGEVYDGASQSWQSSPDH
                     SHTLLENTSDWQKLGAQVVGGCCRTRPADIADLSAHLK"
     gene            complement(3097..4473)
                     /locus_tag="VBC94_001255"
     CDS             complement(3097..4473)
                     /locus_tag="VBC94_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO6979530.1"
                     /translation="MENHRYEDEGKFQRKMTSRHLFMLSLGGVIGTGLFLSSGYTIAQ
                     AGPLGAVLSYLIGAVVVYLVMLSLGELAVAMPVTGSFHTYATKFISPGTGFTVAWLYW
                     ICWTVALGTEFLGAAMLMQRWFPNVPAWAFASFFALVIFGLNALSVRFFAEAESFFSS
                     IKVIAIIIFIILGLGAMFGLVSFEGQHKAILFTHLTANGAFPNGIVAVVSVMLAVNYA
                     FSGTELIGIAAGETDNPKEAVPRAIKTTIGRLVVFFVLTIVVLASLLPMKEAGVSTAP
                     FVDVFDKMGIPFTADIMNFVILTAILSAGNSGLYASSRMLWSLANEGMLSKSVVKINK
                     HGVPMRALLLSMAGAVLSLFSSIYAADTVYLALVSIAGFAVVVVWLAIPVAQINFRKE
                     FLKENQLEDLSYKTPFTPVLPYITIILLLISIVGIAWDSSQRAGLYFGVPFIIFCYIY
                     HKLRYKKW"
     gene            complement(4603..5253)
                     /locus_tag="VBC94_001256"
     CDS             complement(4603..5253)
                     /locus_tag="VBC94_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HEO6979531.1"
                     /translation="MSYFRNYWYRFGAILFIILAVILLVFRPDWSMLHYLLYFYFMAL
                     LAHQFEEYQFPGGASPIINYVVYDEEELMDCFPGNTQSIMLVNTIAWLLYIASIAFPQ
                     AYWLGLGVMFFSLTQLLGHGFQMNIKLKTWYNPGLATTVFLLVPIACAYIYQASAEGM
                     LTWGDWLGGFIMLIVCVLTSIIAPVQLLKDKETNYIISPWQMDRFHKVVNFVRIKK"
     gene            complement(5265..5914)
                     /locus_tag="VBC94_001257"
                     /pseudo
     CDS             complement(5265..5914)
                     /locus_tag="VBC94_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645479.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
     gene            6006..6554
                     /locus_tag="VBC94_001258"
     CDS             6006..6554
                     /locus_tag="VBC94_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO6979532.1"
                     /translation="MTESVTVNKKRIKQITQTQEDILTAFGQLLEEYPYEKIQISQIA
                     KKSGYARRTFYRHFDSRDDLLTLFIERLTLNLFKQLGQLEQPTFSQVFQNFFSYWSDY
                     KSLLLILRKNDLLPQFQQSWFRHIDLIELGRGDLSSNTYAQRFAIGGIFSVLIEWIHQ
                     DCQTSIEELTQVSFDIINHLKN"
     gene            complement(6616..7212)
                     /locus_tag="VBC94_001259"
     CDS             complement(6616..7212)
                     /locus_tag="VBC94_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEO6979533.1"
                     /translation="MAEEFLNTHNASILLSAANKSHYPQDDLPEVALAGRSNVGKSSF
                     INTLLGRKNLARTSSKPGKTQLLNFYNIDDKLRFVDVPGYGYAKVSKTERAKWGKMIE
                     EYLVTRDNLRVVVSLVDFRHDPSADDIQMYEFLKYYEIPVIIVATKADKIPRGKWNKH
                     ESSIKKKLNFDKKDHFIVFSSVDRTGLDESWDTILSEL"
     gene            complement(7223..8449)
                     /gene="clpX"
                     /locus_tag="VBC94_001260"
     CDS             complement(7223..8449)
                     /gene="clpX"
                     /locus_tag="VBC94_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEO6979534.1"
                     /translation="MAGNRNNDMNVYCSFCGKSQDEVKKIIAGNGVFICNECVALSQE
                     IIKEELAEEVLADLAEVPKPKELLEILNQYVVGQDRAKRALAVAVYNHYKRVSYTESS
                     DDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENIL
                     LKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVA
                     SVPPQGGRKHPNQEMIQINTKNILFIVGGAFDGIEDLVKQRLGEKVIGFGQTSRKIDD
                     NASYMQEIISEDIQKFGLIPEFIGRLPVVAALELLTAEDLVRILTEPRNALVKQYQTL
                     LSYDGVELEFDQDALLAIADKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVRI
                     TKAAVEGTDKPVLETA"
     gene            complement(8667..9161)
                     /locus_tag="VBC94_001261"
     CDS             complement(8667..9161)
                     /locus_tag="VBC94_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO6979535.1"
                     /translation="MTKQIIAIWAEDEDHLIGVNGGLPWRLPKELHHFKETTMGQALL
                     MGRKTFDGMNRRVLPGRETIILTKDEQFQADGVTVLNSVEQVIKWFQEHNKTLFIVGG
                     ASIYKAFLPYCEAIIKTKVHGKFKGDTYFPDVNLSEFKVISRDYFEKDEQNAHAFTVT
                     YFEK"
     gene            complement(9241..10080)
                     /locus_tag="VBC94_001262"
     CDS             complement(9241..10080)
                     /locus_tag="VBC94_001262"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEO6979536.1"
                     /translation="MTKADLLFKDNITKIMSEGVFSEQARPRYKNGEMANSKYITGAF
                     AEYDLSKGEFPITTLRPIPIKSAIKEIFWIYQDQTNDLAVLNDKYGVTYWNDWEVGHT
                     GTIGQRYGAVVKKHNIISKLLKQLEDNPWNRRNVISLWDYEAFEETEGLLPCAFQTMF
                     DVRRVNGELYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWRVGKFFYFINNL
                     HIYDNQFEQAQELLKRQPSECNPKLVLNVPDGTDFFDIKPDDFALVDYDPIKPQLRFD
                     LAI"
     gene            10129..11406
                     /locus_tag="VBC94_001263"
     CDS             10129..11406
                     /locus_tag="VBC94_001263"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEO6979537.1"
                     /translation="MRNKTLKVKAFFHIFSFFMVKWNLAKMTFIQKGLPMKIGIDKIG
                     FATSQYVLEMTDLAIARQVDPEKFSKGLLLDSLSITPVTEDIVTLAASAANDILSDED
                     KETIDMVIVATESSIDQSKAASVYVHQLLEIQPFARSFEMKEACYSATAALDYAKLHV
                     EKHPDSKVLVIASDIAKYGIKSTGESTQGAGSIAMLISQNPSILELKEDHLAQTRDIM
                     DFWRPNYSDVPYVNGMFSTKQYLDMLKTTWKVYQKRFNTSLSDYAAFCFHIPFPKLAL
                     KGFNKILDNNLDEQKKAELQENFEHSITYSKKIGNCYTGSLYLGLLSLLENSQNLKAG
                     DQIAFFSYGSGAVAEIFTGQLVDGYQNKLQSDRMDQLNKRQKITVTEYEKLFFEKTIL
                     DENGNANFNTYRTGTFSLDSICEHQRIYKKINN"
     gene            11408..12691
                     /locus_tag="VBC94_001264"
     CDS             11408..12691
                     /locus_tag="VBC94_001264"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEO6979538.1"
                     /translation="MTKISWTGFSKKSPEERIHYLEEQDFLADSSLEIVTNQDLLSLS
                     LANQMAENVIGRIALPFSLVPDVLVNGKVYQVPYVTEEPSVVAAASFAAKIIKRSGGF
                     LTTVHNRKMIGQVALYDVQDSQHTKESILNQKQQLLEIANAAHPSIVKRGGGACDLTI
                     EIKEDFLIVYLMVDTKEAMGANMVNTMMEALSSPLEDISKGKSLMSILSNYATESLVT
                     ATCRVDLRFLSRQKEEAIKLAQKMTMASQLAQVDPYRASTHNKGIFNGIDAIVLATGN
                     DWRAIEAGAHTYAVKDGQYRGLSRWSYKVDDNCLEGTLTLPMPVATKGGSIGINPSVH
                     LAHDLLGRPNAKELASIILSIGLAQNFAALKALVSTGIQAGHMKLQAKSLALLAGAKE
                     EQISEVVKQLLDSKHMNLETAQKIVNKLTKSKKRI"
     gene            12783..13232
                     /locus_tag="VBC94_001265"
     CDS             12783..13232
                     /locus_tag="VBC94_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEO6979539.1"
                     /translation="MNKNQYPKWEELPELDLYLDQVLLYVNQLINPKTITNDKLLTAS
                     MINNYVKHNYISKPIKKKYNRRQVARLIVITAFKQVFAIQEISQTLELLTADNHSEEA
                     YNGFAACMNKEEVHDLPPVVISACQTLNLYQETQKLVLELGGKNKNA"
     gene            13225..13881
                     /locus_tag="VBC94_001266"
     CDS             13225..13881
                     /locus_tag="VBC94_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEO6979540.1"
                     /translation="MLKEMSIKLSPQLSFGEELANSITHAVGALLMLILLPITAVYSH
                     NHFGLQAALGTSIFVTSLFLMFLSSSIYHSMTYNSLQKYVLRMIDHSMIYIAIAGSYT
                     PVALSLIGGWLGYLIIFLQWGITLFGILYKIFAPKINDKFSLVLYLIMGWLVIFIFPA
                     IITKTGPAFWGLLLAGGICYTIGALFYARKRPYDHMIWHLFILLASILQYIGIVYFML
                     "
BASE COUNT         4618 a         2763 c         2448 g         4070 t
ORIGIN      
        1 tttaattctc taatgggaaa tatggggggc tataacagtg agaataagcg atacttaatc
       61 aatggacggg aagttactcc tgaagaattc agtcaatatc gtcaaactgg aaaactcccc
      121 ggtcaagagc taaataacca aaacactcct acaaaccaag tctctgcaga tagtgttttg
      181 actaaattag gaactaattt aaccgatcaa gcacgtcagc atttgctaga tccagttatt
      241 ggacgaaaca aagaaattca ggaaactgca gaaattctag ctcgtcgtac taaaaataat
      301 cctgttctgg ttggtgatgc aggtgttggt aaaacagctg ttattgaagg tttggcacaa
      361 gcaattataa atggtgatgt ccctgctgct atcaaaaata aagaaatcat ttcgatagat
      421 atttctagcc ttgaagctgg cacccaatat aggggtagtt ttgaagaaaa tattcaaaat
      481 atcataaaag aagttaagga aacaggaaat attattctct tctttgatga aatacaccaa
      541 atattaggag ccggttcaac tggtggtgat agtggctcta aggggctcgc agatatactt
      601 aagcctgcac tttcacgtgg tgagttaacg gttataggtg caactactca agatgaatat
      661 cgtaacacca ttctcaagaa tgctgcctta gcacgtcgct ttaacgaagt taaagtcaat
      721 gcaccttctg cacaagatac tttcaatatc cttatgggga taagaaacct ctatgagcaa
      781 catcataatg ttgttttacc tgactctgtt ttgaaagcag ctgtagattt atctattcaa
      841 tacatcccac aaagaagtct ccctgataaa gcaattgacc ttattgatat gactgctgca
      901 cacctagctg cgcaacatcc cgtaactgac cttaaatcct tagaaaaaga aatcgctgag
      961 caaagagaca agcaagaaaa agctgtcaat actgaagatt ttgaagaagc tcttaaggtc
     1021 aagacacgca ttgaagagtt acaaaaccag attgataatc ataccgaagg acaaaaagtc
     1081 actgcaacta tcaacgatat cgctatgtca attgagcgtc taactggagt tcctgtatca
     1141 aatatgggtg ctagtgatat tgaacgctta aaagaacttg gcaatcgtct aaaaggtaaa
     1201 gtcattggtc aaaatgatgc tgttgaggca gttgcgagag ctattcgccg aaatcgtgct
     1261 ggtttcgatg atggtaatcg tccgattggt agctttctat ttgttggacc aactggagtt
     1321 ggtaaaaccg agctagcaaa gcaattagct tttgatatgt tcggatcaaa ggatgctatc
     1381 gttagacttg atatgtctga atataatgat cgtacagcag tttctaagct aattggagcg
     1441 actgctggtt acgttggtta tgacgacaat agtaatacac taacagaacg tatccgtcga
     1501 aatccttatt ctattgttct tcttgatgaa atcgaaaaag ctgacccgca agttatcacg
     1561 cttctccttc aagttctaga tgatggccgt ttaactgatg gtcaaggaaa cactattaac
     1621 tttaaaaata ctgttatcat tgcaacctca aatgcaggtt ttggaaacga agcatttaca
     1681 ggtgacagcg ataaagactt gaaaattatg gaacgaattt ctccatattt ccgtccagaa
     1741 tttctaaatc gtttcaatgg tgttattgaa ttctctcacc taagcaaaga tgacttaagc
     1801 gaaattgtag atttgatgct tgatgaagtt aaccaaacaa ttggcaaaaa aggaattgac
     1861 cttgtggtag atgaaaatgt taaatcacac ttaattgaac tgggttatga cgaagcaatg
     1921 ggagtacgtc cattgcgccg tgtcatcgag caagaaattc gagatcgcat cacagactac
     1981 tatctcgatc atacagacgt taaacaccta aaagctaatt tgcaagatgg ccaaatcgtc
     2041 atttctgaaa gataaaaaca aaacccgttt ggaagaatcc aagcgggttt tctagtggat
     2101 tgaaaaagga aatcaaactt accctaacct tcttcaagat actttactta ttttaaatgt
     2161 gcagagaggt cagcaatatc agctggtcta gttcggcaac aacctccgac aacttgggcg
     2221 ccaagttttt gccagtcact tgtattttca agtagggtgt gtgaatgatc agggctagat
     2281 tgccaagatt ggcttgcccc atcataaacc tctccagagt ttggataggt cacaagaggc
     2341 ttgttagtct gctctgctat tgcttgcaag aaatcagcaa ctaacgatgg acttgaacag
     2401 ttaatgccca gcgccacgac ctgtgggcta acatcaatag ctttcgccaa gtctgcaaca
     2461 gcactaccat ctgagatggt cttaccatct tgagaggtga atgacatata agcctcaacc
     2521 tgagggaaat cctcggcaag tagctcaata agagcctcag cctcctgagc gtttggaatg
     2581 gtttcaagtg ccagaatgtc aacaccctca tctaaaagaa gctcaatacg atggcgatgg
     2641 aagtttttaa gaccttgctt atcaatatcg tagagtccag tgtactcaga gccatccgct
     2701 aaaaaggcag cgtagggacc aacatcccca gaaatcaaag gatagattct ctctgatttt
     2761 tcttcttttg tcaaactttt ccaaacttgc tcacgcgcag cttttgccaa ctgaacagtc
     2821 aagcgaatca aatcttctgc ctgactttca gagacaccaa cttgagctag tccttgtaaa
     2881 gtcgcctgat aagttgatgt cgtcacaata tcagcgccag cacgaatata atcttcatga
     2941 atggtctgaa tagctgctgg atcctcaatc agatatttgg ctgaccaaag tttcccagaa
     3001 acatcacaac ctctactctc aagctcggtc cccaaggcac catgtaggat gagagttttt
     3061 ttactctcta aaagttcttt aaatcttccc ataagatcac catttcttgt aacgtagttt
     3121 gtgataaata tagcagaaaa taataaatgg aacaccgaag taaagcccag cacgttgtga
     3181 gctatcccaa gcaatcccta caatcgaaat caagagcaaa atgattgtga tataagggag
     3241 aactggtgta aaaggtgttt tgtaagacaa atcttctagt tgattctctt tcaagaattc
     3301 cttgcggaaa ttgatttgtg cgactggaat ggctagccat acgacaacaa cagcaaagcc
     3361 cgcgattgaa accaaggcta gataaactgt gtctgcagcg taaatacttg aaaagagcga
     3421 cagcactgct cctgccattg acaagagaag agcacgcatt gggacaccgt gtttattgat
     3481 tttcacaaca gattttgaca acataccttc attggcaagg gaccagagca tacggcttga
     3541 tgcgtagaga cctgagttac cagcagacag gatggctgtc aagataacga agttcatgat
     3601 atccgccgta aaagggattc ccatcttgtc aaagacatca acgaatggtg ctgtggatac
     3661 gcctgcctct ttcattggca atagcgaagc taggacaaca attgtcagta caaagaaaac
     3721 aaccaagcga ccgattgtcg ttttaatagc ccttggtaca gcttctttgg gattatccgt
     3781 ttcaccagcc gcaataccaa ttaactcagt accagagaag gcatagttaa cagccaacat
     3841 gactgagaca actgcaacga taccatttgg aaaggcacca ttggcagtca gatgagtgaa
     3901 gagaatagcc ttgtgctgac cttcaaagga aactagacca aacatagcac ctaagcccaa
     3961 gataataaag ataatgatag caataacctt aatacttgag aagaaagact ctgcttctgc
     4021 aaaaaagcgt acgctaagag catttaaacc aaaaatcaca agggcaaaaa aggaagcaaa
     4081 tgcccaagcc ggcacatttg ggaaccagcg ctgcatcagc atggcagcac ctaaaaattc
     4141 agtccccaag gcgaccgtcc aacaaatcca atatagccaa gcaacagtaa aacctgttcc
     4201 aggactgata aacttagtgg cataagtgtg gaatgacccc gtcaccggca tggcaaccgc
     4261 caattcccca agtgatagca tgaccaaata aaccacaacg gcaccaatca aataagacag
     4321 cacagctcca agcggaccag cctgtgcaat ggtataacct gaactcaaga aaagcccagt
     4381 cccgataaca ccacctagcg ataacataaa gagatgacga ctggtcatct tacgctggaa
     4441 tttaccttca tcttcataac gatgattttc cataacaacc tccaagaaac taatactttt
     4501 atcctaccac aatctagtcg tattggggaa taggtattag ctatcatata tgataaagaa
     4561 aaactattac aaaaaatcag cgaatcataa atagcggtaa ccttattttt ttatccttac
     4621 aaaattaacg accttatgaa aacggtccat ttgccaagga ctaataatat aattggtctc
     4681 cttatccttc aatagctgta caggtgcaat aatgctagtt agtacacaga caatcaacat
     4741 gataaaacca cctagccaat ctccccaagt gagcattcct tctgcactag cttgatagat
     4801 gtatgcgcaa gctattggta ctaggagaaa tactgtcgtt gctagaccag gattatacca
     4861 agttttaagt ttaatattca tctgaaaacc atgacccaag agctgcgtta gactaaagaa
     4921 catgactcct aatccaagcc aataagcttg aggaaaagca atactagcaa tgtaaagcaa
     4981 ccaagcaata gtattaacca acataataga ctgagtattg cctggaaaac aatccatcag
     5041 ctcttcttca tcataaacaa catagttaat gataggtgat gccccaccgg gaaactgata
     5101 ttcttcaaat tgatgcgcta gaagtgccat aaagtaaaaa tacaatagat agtgaagcat
     5161 tgaccagtca ggtctaaaaa caagcaatat tactgctaaa ataataaata aaattgctcc
     5221 aaaacgatac cagtaatttc taaaatatga catgataata cctcctattt aaactgtgtt
     5281 tcacgaaaat gtgttaaaaa tggttttgag cgtgaaagat caacatcact aaatgcgtaa
     5341 tttgaatgtc gacctaaacg cttatagatt ggggaacgaa aggctataaa gtagttaaga
     5401 acaatccata tcagcctaaa aaccaatcaa gtccagaata caacttccaa tcatgagcta
     5461 aataatagac agaaacaggg acaagaccta ctaaggtcgt aagaagtcct ggattatacc
     5521 acttcttcaa ggaaagatta aaaaagaaaa gatgcatggc aagttctgca aatgcaaaaa
     5581 tcatgactgc aagtgttaaa aatggtatat tgggacaaaa aatcggcaaa agataaacaa
     5641 tcactgcaaa ccactgattc ccgaaaaagg ctgatagatg gtttaagtcc cacttactag
     5701 tatcagtttc aaccgattta agttcaacta gcatagtgat aagcggaaaa cctccaggga
     5761 aaccaaattc ttcgaaaaag tgtaattgaa taaaaagtcc cgaaataagt agtgactgca
     5821 ctcgtaaatc ccaatttcca aaaaataatg ctaaggcaaa aagagaagca agatagggac
     5881 aaaagaggta ccattttgtt atcaatgctt tcataagatg acctccaaga tttgtgacat
     5941 ttgataccat atagtataat caagtgaacc tataatatca atacctttgg aggttttatg
     6001 cacaaatgac tgaaagtgtg acagttaata aaaagagaat aaaacaaatc actcaaacac
     6061 aagaggatat cttaacagct tttggccaac ttttggaaga gtatccctat gagaaaattc
     6121 agattagcca aattgccaag aaatctggct atgctagaag gaccttttac cgacactttg
     6181 acagtcgaga tgacctactt acccttttca tcgaaagact aacattaaat ctctttaagc
     6241 aacttggtca attggagcaa cctactttta gccaagtttt ccaaaatttt tttagttatt
     6301 ggtctgatta taaatccctt cttctcattc ttagaaagaa tgacctactc cctcaatttc
     6361 aacaatcctg gttccgacat attgatttga tagagttagg tcgtggtgat ttatcttcca
     6421 atacctatgc tcagcgattt gcgattggtg gtattttcag tgttctaatt gagtggattc
     6481 atcaggactg ccaaacaagt atcgaagaat taactcaagt ttccttcgat attatcaatc
     6541 atttaaaaaa ttaaaaagag ctttgcagta gtctcaacag ctactgcaaa gctctttttg
     6601 tcataaactc tcaaattata actcagataa aatagtatcc catgattcat caagacctgt
     6661 gcggtcaaca gatgaaaaga cgatgaaatg gtccttttta tcaaagttta atttcttctt
     6721 aatagacgac tcatgtttat tccacttgcc acgaggtatt ttatctgcct ttgtcgctac
     6781 aataattact ggaatctcat agtacttaag aaattcatac atttgaatat cgtccgcact
     6841 tggatcatga cgaaaatcaa ctaaactaac aacaacacga aggttatcac gtgttacaag
     6901 gtactcttca atcatcttgc cccacttagc acgctcagtt ttagacacct tagcgtagcc
     6961 atatccaggg acatcgacaa aacgcagttt gtcatcaatg ttataaaagt tgagcagttg
     7021 tgtcttacct ggcttactcg acgtacgcgc taggttttta cgaccaagaa gcgtgttgat
     7081 aaagcttgat ttaccaacat ttgaacgacc agcgagagca acttctggga gatcatcctg
     7141 aggataatgt gacttgttag ctgcactcaa taaaatagac gcattatgag tatttaaaaa
     7201 ttcttctgcc atctctatct ccctaagccg tctctaaaac aggcttgtca gtaccctcaa
     7261 cagcagcctt tgtgatacga acttttgtta catcttcttg gcttggaatt tcaaacatga
     7321 tatcaagcat cgtttcttca ataatagaac gtaaaccacg tgcaccagtc ttgcgctcga
     7381 tagccttatc agcgatagcc aatagagcat cctggtcaaa ttccaattct acaccatcat
     7441 aagataataa ggtttggtat tgtttaacca aagcattgcg tggttctgtc agaatacgaa
     7501 ccagatcttc tgcagtaaga agttctaacg ctgcaactac tggtaaacgg ccaataaact
     7561 ctggaatcag tccaaacttt tgaatatcct cagaaattat ctcttgcata taagaagcgt
     7621 tgtcatcaat tttacggctt gtctgtccaa aaccaataac tttttcgcct aaacgttgct
     7681 tcacaaggtc ttcaatacca tcaaaagcac caccgacaat aaaaaggatg ttcttggtat
     7741 taatttgaat catttcttgg ttaggatgtt tacgcccacc ctgtggggga acacttgcta
     7801 ccgtaccctc aataatttta agaagggctt gctgtacacc ttcaccagac acatcacgtg
     7861 tgatagaaac attttcgcct ttcttagcaa ttttatctat ttcatcaacg tagataatac
     7921 cacgctcagc acgttcgaca ttataatcag cagcttgaat caatttaaga agaatattct
     7981 caacatcttc tccaacgtat cctgcttcgg tcaatgaagt cgcatctgca atagcaaacg
     8041 gtacattaag gcttttagcc agtgtttgtg ctaagaaggt ttttcctgag ccagttggac
     8101 caatcatcaa aatgttggat ttttgcaaat ctacatcatc gtcactactc tcggtataac
     8161 taacacgctt gtaatgattg tagacagcaa ctgctaaagc acgtttagca cgatcttgcc
     8221 ctacaacata ttgatttaat atttctaata gttccttagg ttttggtact tctgctaaat
     8281 cagccagtac ttcctcagct aattcttcct taataatttc ttgtgataag gccacacatt
     8341 cattacaaat gaaaacacca ttacctgcaa taattttttt tacttcatct tggcttttgc
     8401 cacaaaatga acaatagaca ttcatatcgt tattacgatt cccagccatt ttttcctcaa
     8461 cttttctatt catttaattt aaaataatgt tccataaaag gcatggattg tgttgacaag
     8521 gttagtgaaa gcattcatca aaaaaactgt tcctaatcca tatctttttt tctttatagc
     8581 aggaattgca cataaaacac aaatcacagc taaaaatgcg gtaaagaccc caaaaaggct
     8641 acgttgcatt gaatagtatt ccttccttat ttttcaaaat atgtcacagt aaaagcatga
     8701 gcattctgct catccttttc aaaataatct ctagagataa ctttaaactc agatagatta
     8761 acatcaggaa aataggtatc acctttgaat tttccatgaa ctttagtttt tatgattgct
     8821 tcacaataag gcagaaatgc tttataaata cttgcaccac ctacaataaa taaggtctta
     8881 ttatgttcct gaaaccattt tataacttgt tcaacactat ttaggactgt cactccatct
     8941 gcttggaatt gttcatcttt tgttaagatg attgtctctc taccaggtaa aacacgacgg
     9001 ttcattccat caaaggtctt tcgtcccata agcaaagcct gccccatggt cgtttctttg
     9061 aagtgatgta actctttagg aagcctccat ggtaaaccgc cattaactcc aatcaaatgg
     9121 tcttcatctt cagcccaaat ggcaattatt tgctttgtca tgactttttt cctcactaac
     9181 ttatctctag atattctaac aaattttacc tcaaattgct tgttaaaaaa catgagatag
     9241 ttagattgct aaatcaaaac gtaattgagg tttaattggg tcataatcaa ctaacgcaaa
     9301 atcatctggt ttaatatcaa aaaagtctgt tccatctgga acattcaata ctagtttggg
     9361 gttacactca gacggttggc gttttaagag ttcctgagct tgttcaaact ggttatcata
     9421 aatatgaaga ttattgataa aatagaaaaa cttcccaact ctccaaccaa aatgctttgc
     9481 aatcatcatc tgaagtgcta catactgcat agcattaata tgatgtgcaa ctaacatgtc
     9541 attagaacgt tgtgttaatg tggcatccaa ataaagctca ccattaacgc gtctcacatc
     9601 aaacatcgtt tgaaaagcac aaggtagtag gccttctgtt tcttcaaatg cttcatagtc
     9661 ccataatgaa ataacattac gacgattcca agggttatct tcaagctgtt ttaagagttt
     9721 tgatataata ttatgttttt taacaactgc accgtagcgc tgacctatcg tgccagtatg
     9781 gccaacttcc caatcattcc agtatgtcac accatattta tcattcaaaa ctgctaaatc
     9841 atttgtctga tcttgataaa tccagaaaat ttctttaata gcagatttta ttggaattgg
     9901 acgtagagtt gtaataggaa attcaccctt tgataaatca tactctgcaa aggcacctgt
     9961 aatatactta gaattagcca tttccccatt tttatatcta ggtctggctt gctcgctaaa
    10021 tacaccttca gacataattt tcgtgatatt gtctttaaat aataaatcgg cttttgtcat
    10081 gagatacctc caaagtctta ttttatcaga ttttaaaaag atattctagt gagaaacaag
    10141 actttgaagg ttaaagcttt ttttcatatt tttagcttct ttatggtaaa atggaattta
    10201 gcaaaaatga cttttattca gaaaggttta ccaatgaaaa taggaattga taagattggt
    10261 tttgcaacca gtcaatatgt ccttgaaatg actgatttgg ctattgctcg ccaagttgac
    10321 cctgaaaaat tcagcaaggg attattgtta gattcactta gtattacgcc agttacagaa
    10381 gatatcgtta cacttgcagc ttctgcagct aatgatattt tatctgatga ggacaaagaa
    10441 actattgata tggtgattgt cgctacagaa tcaagtatcg atcagagtaa ggcggcttct
    10501 gtctatgtac atcaactttt agagattcaa cctttcgcaa ggtcattcga gatgaaagaa
    10561 gcatgctata gcgcaactgc tgctcttgat tacgctaaat tacatgtaga aaaacatcca
    10621 gattctaaag tattagtgat agcttcagat attgctaaat atggcattaa atctactgga
    10681 gaatccactc aaggtgcagg cagtatcgct atgcttatta gtcaaaatcc atccattcta
    10741 gagctaaaag aggaccatct agcgcaaacc cgtgatatta tggacttttg gcgaccaaat
    10801 tattctgatg ttccttatgt taatggcatg ttttcaacaa aacaatatct agatatgcta
    10861 aaaactactt ggaaagtata tcaaaaacgt tttaatacta gtctaagtga ctatgctgct
    10921 ttttgtttcc atattccttt tccaaaatta gctttaaagg gctttaataa aattctagat
    10981 aataacttag atgaacaaaa gaaagctgaa ctacaagaaa attttgagca ctctattacc
    11041 tatagtaaga aaattggaaa ttgctatact ggctcattgt atctaggact tctatcgtta
    11101 ctagaaaata gtcaaaattt aaaagcaggt gatcaaattg ccttcttttc atacggtagc
    11161 ggagctgttg ctgaaatttt taccggtcag ttagtagacg gctaccaaaa taaacttcag
    11221 agcgatcgta tggatcaact caataaacgt caaaaaatta cagtaactga gtatgagaaa
    11281 ctcttctttg aaaaaacaat cctagacgaa aatggaaatg ctaacttcaa cacctaccgg
    11341 actggcactt tttctctaga ctctatttgc gaacatcaac gtatctacaa gaaaattaat
    11401 aattaatatg acaaaaattt cctggacagg attttcaaaa aaatcccctg aagaacgtat
    11461 tcattacctt gaagaacaag attttttagc tgattcttcc ctagaaatag tgacgaatca
    11521 ggacttactc tctttatcac tagccaatca aatggctgaa aatgtcatcg gtcgaatagc
    11581 cttaccattt tcacttgtcc cagatgtttt ggttaacggt aaagtctatc aagtcccata
    11641 tgtcactgaa gaaccgtcag ttgttgctgc tgcaagtttt gctgctaaaa ttataaaacg
    11701 atctggtggt tttctgacaa ccgtccataa tcgtaaaatg attggacaag tagcccttta
    11761 tgatgtacaa gatagtcaac acactaaaga atctatatta aatcaaaaac agcaattatt
    11821 agaaatagcc aacgctgctc atccatcaat agtcaagcgt ggaggcggtg cttgcgacct
    11881 aactattgag attaaagaag actttttgat tgtttacctt atggttgata caaaagaagc
    11941 catgggtgct aatatggtta acaccatgat ggaagcatta tcttcgccat tagaagacat
    12001 ttcaaaaggc aaaagcttaa tgtcaatttt atctaactat gctaccgaat ctttagtgac
    12061 tgctacatgc cgagttgatt taagatttct aagtcgccaa aaagaggagg ccatcaagtt
    12121 agctcaaaaa atgacgatgg caagtcaatt agctcaagta gacccttatc gtgctagcac
    12181 acataataaa ggtattttta atggcattga tgctatcgtc ttagctactg gaaatgattg
    12241 gcgagctatt gaagctggag cgcataccta tgctgtcaaa gatgggcaat atcgaggatt
    12301 atctagatgg tcttataagg ttgatgataa ttgcctagaa ggtactctga cgctaccgat
    12361 gcctgttgca acaaaaggtg gatccattgg tattaaccct agtgtccacc tagctcacga
    12421 tctactaggt cgtcctaatg ctaaagagtt ggcaagtatt attctttcga ttggactagc
    12481 tcaaaatttt gcggctctta aggcattggt tagcacagga attcaagctg gtcacatgaa
    12541 acttcaagca aagtcgttgg cactacttgc tggagctaaa gaagaacaaa tttcagaagt
    12601 cgttaaacag cttctagact ctaaacacat gaatctagag acagcacaaa aaattgtcaa
    12661 taaacttact aaaagtaaaa aaagaatttg agaaaaatca tctcaaattc tttttttact
    12721 tgacttgaat ttccataagt gtataatcta gttataaaga ctagattata tggagtttta
    12781 aaatgaataa aaatcaatac ccaaaatggg aagaattacc tgaactagat ttgtatcttg
    12841 accaagtact tctctatgtg aatcaattaa ttaatcctaa aacaataact aacgataaac
    12901 tactcaccgc ttcgatgatc aacaattatg tcaaacataa ttatatatcc aaacctataa
    12961 aaaaaaaata caatcgccgt caagtagcac gattgattgt tattacagct tttaaacaag
    13021 tttttgctat tcaagaaatc agtcaaacat tagaacttct gacagctgac aatcattctg
    13081 aagaagcata taacggattt gctgcttgta tgaacaaaga agaggttcac gatttaccac
    13141 cagttgtcat tagtgcctgt cagacgttga acctttatca agaaacacaa aaattagttt
    13201 tagaactagg aggaaaaaat aaaaatgctt aaagaaatgt ctatcaaatt aagtcctcaa
    13261 ctttcctttg gcgaagagct tgcaaatagt atcactcacg ctgtaggagc tttgcttatg
    13321 ctaatcctat taccaatcac cgctgtctat agtcacaacc acttcggatt acaagcagca
    13381 cttggtacct caatattcgt cacttcttta tttttgatgt ttttatcatc aagtatttac
    13441 cactccatga cttataacag tcttcaaaaa tatgttctac gcatgattga tcactctatg
    13501 atttatattg ctattgcagg tagttatact cctgtcgcat tgtcacttat tggcggctgg
    13561 ttaggatatc tcatcatttt tttacaatgg gggattacat tatttggtat cctttataaa
    13621 atcttcgctc ccaaaatcaa tgataaattt agccttgtac tttacctcat tatgggatgg
    13681 ctggttatct ttattttccc tgctattatc acaaaaactg gaccagcttt ttggggatta
    13741 ctcttggctg gtggtatttg ctatactatc ggtgcactct tttatgctcg caaacgtcct
    13801 tatgatcaca tgatttggca cctttttatt ctattagctt ctattttaca atacattgga
    13861 attgtctact tcatgctata aaatttaact acattttat
//
LOCUS       DATROQ010000048        13874 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.049, whole genome shotgun sequence.
ACCESSION   DATROQ010000048 DATROQ010000000
VERSION     DATROQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13874
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.049"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            63..653
                     /locus_tag="VBC94_001267"
     CDS             63..653
                     /locus_tag="VBC94_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6979541.1"
                     /translation="MANMYYTENPNVEHDIHELNVKLLGESFSFLTDAGVFSKRMIDY
                     GSQVLLNSLHFEKNKSLLDLGCGYGPLGISLAKVQGVKATMIDINTRALELAKKNATR
                     NGVVVEVFQSNIYENISKTFDYIISNPPIRAGKQVVHSIIEESICYLNTGGSLTIVIQ
                     KKQGAPSAKAKMLDTFGNCDILKKDKGYYILRSEKV"
     gene            979..1368
                     /locus_tag="VBC94_001268"
     CDS             979..1368
                     /locus_tag="VBC94_001268"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEO6979542.1"
                     /translation="MGNIELKKLAVKASENAYVPYSKFPVGAALRTAEGKIFTGCNVE
                     NISYGLANCAERTAIFKAVSEGYKDFSEIAIYGNTEEPISPCGACRQVMVEFFNKNAK
                     VTLIAKNGKTVETTVGELLPYSFVDLK"
     gene            1433..2482
                     /locus_tag="VBC94_001269"
     CDS             1433..2482
                     /locus_tag="VBC94_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO6979543.1"
                     /translation="MNKKIAGIGLASIAVLSLAACGHRGASKSGGKSDSLKVAMVTDT
                     GGVDDKSFNQSGWEGMQAWGKKNGLKKGAGFDYFQSASESDYATNLDTAVSSGYKLIF
                     GIGFSLHDAIDKAADNNKDVNYVIVDDVIKGKDNVASVVFADNESAYLAGIAAAKTTK
                     TKTVGFVGGMESEVITRFEKGFEAGVKSVDKSIKIKVDYAGSFGDAAKGKTIAAAQYA
                     SGADIVYQVAGGTGAGVFSEAKSRNESLKEADKVWVLGVDRDQAAEGKYTSKDGKASN
                     FVLASSIKEVGKSVELIATKTSKGKFPGGNVTTYGLKDGGVDIATTNLSDDAVKAIKE
                     AKAKIISGDIKVPSK"
     gene            2627..4162
                     /locus_tag="VBC94_001270"
     CDS             2627..4162
                     /locus_tag="VBC94_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979544.1"
                     /translation="MTQNVIEMKEITKKFGDFVANDHINLTVEKGEIHALLGENGAGK
                     STLMNMLAGLLEPTDGQIFINGQPVTIDSPSKSSQLGIGMVHQHFMLVEAFTVAENIV
                     LGNETTQNGVLDIKTAAKEIKELSEKYGLSVNPNAKISDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPSEIKELMTIMKSLVKEGKSIILITHKLDEIRAVADKVTVIRRGK
                     SIETVPVAGASSQQLAEMMVGRSVSFRTEKKEANPTDIILSVKDLVVEENRGGVLAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVTSGQIVIKGKDVTKFSSRQITEL
                     SVGHVPEDRHRDGLVLDMTMAENLALQTYYKEPLSHKGILNFAKIKEYARQLMTEFDV
                     RGAGEHVLARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DKGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVKPDQTNKQELGILMAGGKIEKEE
                     RDV"
     gene            4155..5216
                     /locus_tag="VBC94_001271"
     CDS             4155..5216
                     /locus_tag="VBC94_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6979545.1"
                     /translation="MSKKAQKIAVPLISVVLGIILGAIIMLIFGYDPLWGYEGLFQTA
                     FGSIKNIGEIFRAMGPLILIALGFSVASRAGFFNIGLPGQALSGWIAAGWFALSHPDM
                     PRPAMILCTIIIGIVAGGITGAIPGILRAYLGTSEVIVTIMMNYIVLYSGNAIVQRVF
                     PKSIMRTSDSSVYVSANASYQTDWLSSLTNNSRINIGIFIAIIAVVLVWFLLNKTTLG
                     FEIRSVGLNPNASEYAGMSAKRTIILSMIISGAFAGLGGVVEGLGTFENVFVQPSSLA
                     IGFDGMAVSLLAANSPIGILFAAFLFGVLSVGAPGMNIAGIPPELIKVVTASIIFFVG
                     VHYIIEYVIKPKKQMKGGK"
     gene            5218..6174
                     /locus_tag="VBC94_001272"
     CDS             5218..6174
                     /locus_tag="VBC94_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6979546.1"
                     /translation="MSLTTIFALLFSSMLIYATPLIFTSIGGTFSERGGIVNVGLEGI
                     MVIGAFSGVVFNLEFASVFGDATPWISVLVGGLVGLIFSVIHAVATVNFRADHIISGT
                     VLNLMAPSLAVFLIKVLYNKGQTDNIQESFGKFNFPILSDIPFVGDIFFKGTSLVGYI
                     AILFSFLAWFILYKTRFGLRLRSVGEHPQAADTLGINVYLMRYSGVLISGFLGGIGGA
                     VYAQSISVNFAATTILGPGFISLAAMIFGKWNPIGAMLASLFFGLSQSLAVIGSHLPL
                     LSNIPTVYLQIAPYVLTIIVLAAFFGQAVAPKADGINYIKTK"
     gene            6380..7750
                     /locus_tag="VBC94_001273"
     CDS             6380..7750
                     /locus_tag="VBC94_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO6979547.1"
                     /translation="MSKIVVVGTNHAGTAAIKTMLSNYGEANEIVTFDQNSNISFLGC
                     GMALWIGEQIDGPEGLFYSDKEQLESMGAKVYMNSPVLNIDYDKKEVTALVDGKEHVE
                     SYEKLILATGSQPIIPPIKGVEIQEGSREFKATLENLQFVKLYQNSEEVIEKLAKPGI
                     NRVAVVGAGYIGVELAEAFQRIGKEVTLVDVADTCMGGYYDRDFTDMMSKNLEDHGIR
                     LAFGQAVQAVEGDGKVERLVTDKETFDVDMVILAVGFRPNTELGAGKLDTFRNGAWVV
                     DKKQETSVKDVYAIGDCATIWDNSRDDINYIALASNAVRTGIVAAHNACGTELEGAGV
                     QGSNGISIYGLNMVSTGLTLEKAKQAGYNAVETGFNDLQKPEFIKHNNHEVAIKIVYD
                     KDSRVILGCQMVSHEDVSMGIHMFSLAIQEKVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(7871..8860)
                     /locus_tag="VBC94_001274"
     CDS             complement(7871..8860)
                     /locus_tag="VBC94_001274"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO6979548.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPALF
                     DKAVGDAEDLSHALAFTSPKKIYAATYADCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALADKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVQLEQWLQENRDIDEQGLVDLF
                     ISVRDAAYSIINKKGATYYGIAVALARITKAILDDENAVLPLSVYQEGQYGDVKDVFI
                     GQPAIVGAHGIVRPVNIPLNDAELQKMQASAEQLKDIIDEAWKNPEFQEASKN"
     gene            9099..11558
                     /gene="gyrA"
                     /locus_tag="VBC94_001275"
     CDS             9099..11558
                     /gene="gyrA"
                     /locus_tag="VBC94_001275"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEO6979549.1"
                     /translation="MQDKNLVDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVDFQDNYDGSEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGESIDAVKLVMDNPDVTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYETGKGSIVLRSRTEIETTSNGKERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRIEGITAVRDESSREGVRFVIEVRRDASANVILNNLFKLTSLQTNFSFNMLAI
                     EKGVPKILSLRQIIDNYIEHQKEVIVRRTQFDKAKAEARAHILEGLLVALDHLDEVIT
                     IIRNSETDTIAQAELMSRFELSERQSQAILDMRLRRLTGLERDKIQSEYNDLLALIAD
                     LADILAKPERVVTIIKEEMDEVKRKYADARRTELMIGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGIQGTGVNNDDFVRELVSTSTHDTVLFFTNLGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLDEGETIQTIINARKEDVANKYFFFTTQQGIVKRTS
                     VSEFSNIRQNGLRAINLKENDELINVLLIDENEDVIIGTRTGYSVRFKVNAVRNMGRT
                     ATGVRGVNLREGDKVVGASRIVNGQEVLIITEKGYGKRTEASEYPTKGRGGKGIKTAN
                     ITAKNGPLARLVTINGNEDIMVITDTGVIIRTNVANISQTGRSTMGVKVMRLDQEAKI
                     VTVALVEQEIEDKSNIEDTKE"
     gene            11565..12308
                     /locus_tag="VBC94_001276"
     CDS             11565..12308
                     /locus_tag="VBC94_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEO6979550.1"
                     /translation="MRNKKKSHGFFNFVRWLLVVLLIIVGLALVFNKPIRNAFIAHQS
                     NHYQISRVSKKTIEKNKKSKTSYDFSSVKSISTESILSAQTKSHNLPVIGGIAIPDVE
                     INLPIFKGLGNTELSYGAGTMKENQIMGGPNNYALASHHVFGLTGSSKMLFSPLEHAK
                     KGMKVYLTDKSKVYTYTITEISKVTPEHVEVIDDTPGKSQLTLVTCTDPEATERIIVH
                     AELEKTGEFSTADESILKAFSKKYNQINL"
     gene            12324..12737
                     /locus_tag="VBC94_001277"
     CDS             12324..12737
                     /locus_tag="VBC94_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6979551.1"
                     /translation="MKLKAVHHIAIIVSDYEKSKDFYVNKLGFEIIRENHRPERHDYK
                     LDLRCGDIELEIFGNRLDDPEYETPPQRIGRPNWPREACGLRHLAFYVPDVEAYKVEL
                     ENLGIFVEPIRYDDYTGKKMTFFFDPDGLPLELHE"
     gene            12890..13852
                     /locus_tag="VBC94_001278"
     CDS             12890..13852
                     /locus_tag="VBC94_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEO6979552.1"
                     /translation="MSIKKLLFAGLAFILFTLASPAYAASDVQKVIDETYVQPDYVLG
                     YSLNQEQRAQTLQLLNYDESRDTKVKTLNTSSYAKIMNIADDASIQLYSSVKIKKLGS
                     NDTLAVNIVTPENITKVTEDMYRNAAVTLGIEHATISVAAPIKVTGESALAGIYYSLE
                     KNGASVSSENKQLAQEELSTLSGINAENKGKEGYDADKLNVALTDIKSAVAKGGSDLS
                     KDDIRKIVEETLKNYHLDNAVTENQINLIVNFAVNLSQSNVIKNSDFTNTLNNLKDNI
                     VSKAGSKFKNINVNFNANKAVESGKGFLANIWQQIVNFFQQFGS"
BASE COUNT         4532 a         2111 c         2846 g         4385 t
ORIGIN      
        1 attgaaaacc gtttcaaaat atggtaaaat aatatggcga aattggaggg ttagaattat
       61 ttatggctaa tatgtattat acagagaatc ctaatgttga acatgatatt catgagctaa
      121 atgtcaaatt attaggagaa tcattcagtt ttttaactga tgcaggagtt ttctctaaaa
      181 gaatgataga ttatggtagt caagtccttc taaatagttt acattttgaa aaaaataaat
      241 ccttgttaga ccttgggtgt ggatacggtc cgctgggaat ttctctagcc aaagtgcaag
      301 gagttaaagc aactatgatt gacattaata cgagagcatt ggaattagct aagaaaaatg
      361 caacacgcaa tggagtagta gttgaagtct ttcagtcaaa catttatgaa aatatttcta
      421 aaactttcga ctatattata agcaacccac ctattcgtgc tggaaagcaa gttgttcatt
      481 ctattatcga agaaagtatc tgttacctta atacaggcgg tagccttacc attgtcattc
      541 aaaagaaaca aggcgctcca agtgcaaaag ctaaaatgct agatactttt ggtaattgcg
      601 atattttaaa aaaagataag ggatattata tattgagaag tgaaaaagtc taaatgatat
      661 ccctaaagcg taaaaataat aaatagttcg gataataact atcgtattat tttttggaaa
      721 ggttttctct aaaaatgttt tctttaaatt ccgtataaga taaaaagaat tctgcttttt
      781 attctgaaaa ttataaaaaa ctacttacat tatctaaaaa aaatggtaaa atagattcag
      841 ctaaaatacc aagactaatg aaactacaag acttggttac tcaataataa atacttctgt
      901 gtttgataag aagataataa acgtgttcta aaaattttgc cagtaaacat tatgaagcga
      961 aaggtgcagc tactaaaaat gggaaatata gagttgaaaa aattggctgt aaaagcgagt
     1021 gaaaatgcat acgttcctta ctcgaaattt ccagtaggcg cagcattgcg tacagcagaa
     1081 ggaaaaatat ttacaggatg taacgttgaa aatatcagtt atggattagc aaactgtgct
     1141 gagcgtactg caatttttaa agctgtttca gaaggttata aagatttttc ggaaattgct
     1201 atttatggaa atactgaaga accaatttct ccttgcggtg catgccgtca agtgatggtg
     1261 gaatttttta ataagaatgc taaagttaca ttgattgcta aaaatggtaa gacggtcgag
     1321 acgacggtcg gtgagttgtt accatactcg tttgttgatt taaaataatt taaatcagtt
     1381 gctcttttaa gcatttagct taaatattaa gttttttagg agggttcatt tcatgaacaa
     1441 aaaaattgcc gggatcggct tggcttcgat tgcagtactt agtttagctg catgtggaca
     1501 tcgtggtgct tctaaatctg gtggtaaatc agatagcttg aaggttgcaa tggtaacaga
     1561 taccggtggt gttgatgata aatcatttaa ccaatctggt tgggaaggta tgcaagcttg
     1621 gggcaagaag aatggcctta aaaaaggagc tggttttgac tatttccaat cggcaagtga
     1681 atctgattat gcaactaact tagatacagc tgtgtctagt ggttataaat tgattttcgg
     1741 tattggattt tctcttcatg atgctattga taaagcagca gacaataaca aagatgttaa
     1801 ttacgtcatc gttgatgatg ttattaaagg gaaagataat gttgcaagtg ttgtctttgc
     1861 ggataatgaa tcagcttact tagcaggtat tgcagccgct aaaactacca aaacaaaaac
     1921 agttggcttt gtaggtggta tggaatctga ggttattacc cgttttgaaa aaggttttga
     1981 agcaggtgtc aaatcagttg ataaatcaat taaaattaaa gttgactatg ctggttcatt
     2041 cggtgatgct gctaagggta agacaattgc agccgcacaa tatgcttctg gcgcagatat
     2101 tgtttatcaa gtagctggtg gaactggtgc aggtgtattc agtgaagcaa aatcacgcaa
     2161 tgaatcttta aaagaagctg acaaagtttg ggtattaggt gtagaccgtg accaagcagc
     2221 agaaggtaag tatacttcta aagatggtaa ggcttctaac tttgttttgg cttcatcaat
     2281 taaagaagtt ggaaaatcag ttgaattgat tgctactaaa acaagtaaag gtaaattccc
     2341 aggtggtaat gtaacaactt atggtttgaa ggatggtgga gttgatatag caactacaaa
     2401 cctttcagat gacgccgtta aagcgattaa agaggctaaa gctaaaatta tttcaggaga
     2461 tatcaaagtt cctagcaaat aatatcttta agaactttca aaaaagcgat tagaaatcgc
     2521 ttttttgaag ctgagataaa cttatatctc agtaaaatcc atcagtcgtt aacgattggt
     2581 ggattttact gaactataaa tgaatttttc tgaaagggag aaccacatga cacagaatgt
     2641 cattgaaatg aaagaaatca ctaagaaatt cggtgatttt gtggcaaatg atcatatcaa
     2701 tttgacggtt gaaaaaggtg agatacatgc tctgcttggc gaaaacggtg cagggaaatc
     2761 aactttaatg aacatgttag caggtttact agaaccaaca gacggtcaaa tttttattaa
     2821 tggtcagcct gtaactattg attcaccttc caaatcgtca caattgggga ttggtatggt
     2881 acatcagcat ttcatgcttg ttgaagcatt tactgtagct gaaaatattg ttctaggaaa
     2941 tgaaaccacc caaaatggtg ttttagacat taagacagca gctaaagaaa taaaggaact
     3001 atcagagaaa tatggtttgt cagtaaatcc aaatgctaaa atatctgata tttctgtagg
     3061 agcacagcag cgtgttgaaa ttttgaagac actttatcgc ggtgcagata ttttaatttt
     3121 tgatgagccg acggcggtgc ttacaccatc agaaataaaa gaattgatga caattatgaa
     3181 gagccttgtt aaagaaggca aatccattat actaattacc cataaattgg atgaaattcg
     3241 ggcagttgct gataaagtta ctgtgattcg ccgtggtaaa agtattgaaa cagtccctgt
     3301 tgcaggcgca agttctcaac aactggcgga aatgatggtt ggacgatctg tttccttcag
     3361 aactgaaaaa aaggaagcta atccgactga cataatcctt tctgtaaagg atttagttgt
     3421 agaagaaaat agaggcggcg ttctagctgt taaaaatctg tcgttagatg ttcgagcagg
     3481 tgaaattgta ggtatagctg gtattgatgg taatggacaa agtgaattaa ttcaagctat
     3541 tacaggttta agaaaggtaa cttctggtca aattgtaatt aaaggtaaag atgtcacaaa
     3601 attttcaagt cgacaaatta cagaattaag tgtaggtcat gttccagaag atcgtcatag
     3661 agatggtttg gttctagata tgacgatggc agaaaattta gctttacaaa cttattataa
     3721 agagccattg agtcataaag gtatccttaa ttttgctaaa attaaagagt atgctcgtca
     3781 attaatgaca gaatttgatg tgcgtggtgc tggtgaacat gttttagcaa gaggattttc
     3841 tggtggtaac caacaaaaag ctattattgc tcgtgaagtg gatcgcgatc cagacctact
     3901 cattgttagc caaccgactc gtggtctaga tgttggtgct atcgagtata ttcataaacg
     3961 tttgattgaa gagcgcgata aagggaaagc ggtcttagta gttagctttg agttagatga
     4021 aatcttaaac ttatcagatc gtattgcagt tattcatgat ggtaaaattc aaggaattgt
     4081 taaacccgac caaaccaaca agcaagaatt gggtattttg atggctggtg gaaaaattga
     4141 aaaggaggag agagatgtct aagaaggcgc aaaaaattgc agttcctctg atttcagttg
     4201 tactaggaat cattctagga gctattatta tgctgatatt tggttatgat ccactttggg
     4261 gttatgaggg attgttccaa acagcattcg gtagtataaa aaatattggt gaaattttcc
     4321 gtgcaatggg accattaatt ttaattgctc taggtttctc agttgccagt cgtgcaggtt
     4381 tctttaatat tggacttcca gggcaagcct tatcaggatg gattgccgca ggttggttcg
     4441 ctctttcaca cccagatatg ccacgtccag caatgattct ttgtactatt attattggta
     4501 tcgttgctgg gggaatcact ggagctattc caggtattct cagagcatac cttggaacaa
     4561 gtgaagttat tgtaactatt atgatgaact atattgttct ttattcagga aatgctattg
     4621 tacaacgtgt tttcccaaaa agcattatgc gtacaagtga ctcgagtgta tatgtttctg
     4681 ccaatgcatc ataccagaca gattggttat ctagtttaac gaataattca cgtatcaata
     4741 ttggtatctt cattgctatt attgcagttg tgttagtttg gttcctatta aataaaacaa
     4801 cgcttggatt tgaaattcgt tcagttggtt taaatccaaa tgctagtgag tacgctggta
     4861 tgtctgcaaa gcgtacaatt attctatcaa tgattatttc tggagctttt gcaggacttg
     4921 gtggtgttgt tgaaggacta ggtacatttg agaatgtatt tgtacagcca tcctcgttag
     4981 ctattggctt tgacggtatg gcagtaagtc tactcgcagc gaatagtcca ataggtattt
     5041 tatttgcagc atttctattt ggtgttctaa gtgttggtgc gcctggtatg aacattgcag
     5101 gtattccacc tgagttaatt aaagttgtaa cagcttcaat catcttcttc gtaggtgtcc
     5161 attacattat tgagtatgtt attaaaccta aaaaacaaat gaaaggtggt aagtaaattg
     5221 agtttaacaa cgatttttgc attgctattt tcatcaatgc taatttacgc aacacctctt
     5281 atctttacaa gtattggggg aaccttctct gaacgtggtg gtatcgtcaa cgttggttta
     5341 gaaggaatta tggtaattgg agctttctca ggcgttgtat ttaatttaga gtttgcttct
     5401 gtttttggtg atgcgactcc atggatttct gtactagttg gtggtttagt tggtttaatt
     5461 ttttctgtta tccatgcagt tgcgacagtt aattttcgtg cagatcatat tatcagcgga
     5521 accgttctta atttaatggc accttcatta gctgttttct tgattaaggt tctttataat
     5581 aaaggtcaaa ctgataatat tcaagaatca tttggaaaat ttaatttccc tatactatca
     5641 gatattcctt ttgttggtga tatcttcttt aaaggaacaa gcttagtcgg ttatattgct
     5701 attcttttct cattccttgc ttggtttatt ctatataaaa ctcgctttgg attaagatta
     5761 cgctcagttg gagaacatcc tcaagcagca gacaccttag gtattaatgt ttatcttatg
     5821 cgttactctg gtgtcttgat ttcaggtttc cttggtggaa taggaggagc tgtatatgct
     5881 caatctattt ctgttaactt tgcagcgaca actatattag gaccgggctt tatctcctta
     5941 gcggcgatga tttttggtaa gtggaatcca attggtgcaa tgcttgctag tctattcttt
     6001 ggtttatcgc aaagtttagc agttatcggt agccatttac cacttttgag caatattcca
     6061 acagtttatc tacaaattgc tccatatgtc ctcactatca ttgtattagc agctttcttt
     6121 ggacaagcag tagctcctaa ggcggatggt attaattata ttaaaacgaa ataaataaaa
     6181 agaaactagt ataagctagt ttctttttat tgtttgtgaa tttgtgaaaa aaatactttg
     6241 tgaaaatttc acataaatta taatacaata acaataaaat ataaaaaaat agaaaattga
     6301 gtcattattc tagtaagagt ttgtgaataa tgttataatg tgttttgtta ttaattattt
     6361 ataaaggaga actcttaata tgagtaaaat cgttgtcgta gggacaaacc acgctggtac
     6421 agcagcaatt aaaacaatgc ttagtaatta tggtgaagcc aatgaaattg taacatttga
     6481 tcaaaattcc aatatctctt tcttaggatg tggtatggct ctttggattg gtgaacagat
     6541 tgatggtcca gaaggattgt tctactctga taaagagcag ttggaaagta tgggagctaa
     6601 ggtttacatg aactctccag tacttaatat tgactacgat aaaaaagaag ttacagcttt
     6661 agtagatgga aaagaacatg ttgaaagtta tgaaaaactt attttagcaa ctggttcaca
     6721 acctatcatt ccaccaatta aaggtgttga aattcaagaa ggatctcgtg aatttaaagc
     6781 tactcttgaa aaccttcaat ttgtgaaact ttatcaaaat tcagaagaag ttattgaaaa
     6841 gttagctaaa cctggtatca atcgtgttgc tgttgttggg gctgggtata ttggtgtaga
     6901 attagcagaa gctttccaac gtattggaaa agaagtaaca cttgttgatg ttgctgatac
     6961 ttgtatgggt ggttactatg atcgtgactt tactgatatg atgagtaaaa accttgaaga
     7021 ccatggtatt cgtcttgctt tcggacaagc tgttcaagca gttgaaggtg atggtaaagt
     7081 agaacgtctt gttactgata aagaaacatt tgatgttgat atggtgatct tagctgttgg
     7141 attccgtcca aatacagaac tcggtgcagg taaattagat acattccgta atggcgcttg
     7201 ggtagttgat aagaagcaag aaacaagcgt gaaagatgtt tatgctatag gtgattgtgc
     7261 gacaatttgg gataattctc gtgatgatat caattatatt gctcttgctt caaacgccgt
     7321 tcgtactggt attgtagcag ctcacaatgc atgtggtaca gagcttgaag gtgctggtgt
     7381 ccaagggtca aatggtatct caatctatgg ccttaacatg gtgtcaactg gtttgacttt
     7441 agaaaaagca aaacaagctg ggtacaatgc agttgaaaca ggctttaatg accttcaaaa
     7501 accagaattt atcaaacaca ataatcatga agtagctatt aagattgtct atgataaaga
     7561 tagccgagtt atccttggtt gccaaatggt ctcacacgaa gatgtatcta tgggaattca
     7621 tatgttctca cttgctattc aagaaaaagt tactattgaa aagcttgctt tgaccgatat
     7681 cttcttctta ccacatttta ataagccata taactatatt acaatggctg cattaggcgc
     7741 taaagattaa tttaaaatta aaaaggttaa gcgaaatgct tagcctttta tttcatcctt
     7801 taacaaggtc aaaaatgtaa cggcaaagaa aaagctaaga tattcctatc ctagcttttt
     7861 ttcaactatg ttagtttttt gatgcttctt ggaattctgg gtttttccaa gcttcgtcaa
     7921 taatatcttt taattgttca gctgaggctt gcattttttg aagttcagca tcatttaatg
     7981 ggatattaac tggacgaacg ataccatgtg cacctacgat tgcaggctga ccgataaaga
     8041 catctttaac atcaccgtat tggccttctt gatatacaga taatggaaga actgcatttt
     8101 catcatcaag gatagcttta gtaatacgag caagtgcgac agcaataccg taatatgtag
     8161 cacctttttt gttgatgatt gagtatgcag cgtcacgaac tgaaataaac aaatcaacaa
     8221 gtccttgttc atcaatatcg cgattttctt gtaaccattg ttcaagttgg acaccagcaa
     8281 cgttagcatg tgaccaaacg gcaaattctg agtcaccgtg ttcacccatg atgtatgcgt
     8341 gaactgaacg agcatcaaca ccaattttat ctgctaaagc ttgacggaaa cgtgcagagt
     8401 caagtgaagt acctgaacca ataacgcgtt ccttagggaa acctgagaat ttccaagttg
     8461 aatatgtaag tacatctaca gggttagccg ctactaagaa gataccgttg aaacctgatt
     8521 caacaacttg agttacgatt gatttgttaa ttgctaagtt tttaccaaca aggtcaagtc
     8581 gagtttcacc tggtttttga ggtgcgccag cagtaatgac aacaaggtca gcatctgcgc
     8641 agtctgcata agtagctgcg tagatttttt taggtgatgt aaatgcaagg gcatgtgaaa
     8701 gatcttcagc atcaccaaca gctttatcaa ataaagctgg gatttcaatg atacctaact
     8761 cttgcgcaat accttggtta acaagtgcaa aagcgtaaga agaacctacg gcaccatcac
     8821 caacgagaat aactttttta tgttgtttag ttgcagtcat ttctaaacat ctccttaaat
     8881 atttttggga agtttcccat acacctatat tctatcattt tcaatgaaag aagtcactaa
     8941 taaaatgtga aaaaatttgc aaacaaattg aaatatcata gaaaactatt gaaaacgctg
     9001 agtgtcttga tatttttgat accgatttat catcgcaatt ttacctaaat tatggtacaa
     9061 tgtaagagac ttacagtgaa gaaaaggggc attttttaat gcaagataaa aatttagtag
     9121 atgttaatct aactagtgaa atgaaaacga gttttatcga ttacgccatg agtgtcattg
     9181 ttgctcgtgc acttccagat gttagagatg gtttaaaacc tgttcatcgt cgtattttat
     9241 atggtatgaa tgaactaggt gtgacacctg ataaacctca taagaaatca gcacgtatta
     9301 ctggtgatgt tatgggtaaa taccatccac atggtgattc atctatttac gaagcaatgg
     9361 tgcgtatggc acaatggtgg tcatatcgtc atatgctcgt tgatggtcat ggaaactttg
     9421 gttcaatgga tggggatggt gctgccgcac agcgttatac agaagcacgt atgagtaaaa
     9481 ttgctcttga gatgcttcgt gatatcaata aaaatacagt tgattttcaa gataactatg
     9541 atggcagtga acgtgaacct cttgttttac cagcgcgttt tcctaattta ttagtcaatg
     9601 gtgcaactgg tattgctgta ggtatggcaa caaatattcc accacataat ctaggtgagt
     9661 ctatagatgc tgtcaaattg gttatggata accctgatgt tactacacgg gagttaatgg
     9721 aagttattcc tggtccagat tttcctactg gtgccttggt gatgggacgt tcaggtattc
     9781 accgtgccta tgaaactggg aagggatcaa ttgtcttacg ttcacggact gaaattgaaa
     9841 caacttcaaa tgggaaagag cgtattgttg tcacagagtt tccatatggc gttaataaaa
     9901 caaaagtaca cgagcatatt gtacgtttag cacaagaaaa gcgcattgaa ggtattactg
     9961 ctgttcgtga tgaatcaagt cgtgaaggcg ttcgttttgt tattgaagtt cgtcgtgatg
    10021 cttcagcaaa tgtgatttta aataatcttt tcaaattaac tagcttgcag acaaatttta
    10081 gcttcaatat gcttgctatt gaaaaaggtg ttccaaaaat tttgtcatta cgacaaatca
    10141 ttgataacta tattgaacac caaaaggaag ttattgttcg tcgtactcag tttgacaaag
    10201 ctaaagcaga agcccgtgct catatcttag aaggtttact tgtggcgctt gatcacttgg
    10261 atgaagttat cacaattatt cgtaatagtg agacagacac cattgctcaa gcagagttga
    10321 tgtcacgttt tgaattatcg gagcgtcaga gtcaagctat tttagatatg cgtcttcgtc
    10381 gtttaactgg attagaacgc gataaaatcc aatcagaata caatgactta ctagctttaa
    10441 ttgctgattt agcagatatc ttagctaagc ctgaacgtgt tgttaccatt ataaaagaag
    10501 agatggatga ggttaagcgt aagtatgctg atgcccgtcg tacagaatta atgattggtg
    10561 aagttttatc acttgaagat gaagacctta ttgaggaaga agatgttctt attactctct
    10621 caaacaaggg atatatcaag cgtctcgctc aagatgagtt tagagcacaa aaacgtggag
    10681 gacgcggtat tcaagggact ggagttaata acgatgattt tgttcgtgaa ttggtttcaa
    10741 caagtactca cgatactgtg cttttcttta caaatttagg gcgtgtgtat cgtttgaaag
    10801 catatgaaat tccagaatat ggacgtacag ctaaaggcct ccctatcgtt aaccttttaa
    10861 aacttgacga aggtgagaca attcaaacaa ttattaacgc tagaaaagaa gatgtcgcta
    10921 ataaatattt ctttttcaca actcagcaag gtatagtgaa acgtacaagt gtttctgaat
    10981 ttagtaacat tcgtcaaaat ggacttcgtg ctattaattt gaaagagaat gacgagttga
    11041 ttaacgtcct cttgattgac gaaaatgaag atgttattat cgggacacgt acaggctact
    11101 ctgttcgctt caaagtgaac gctgtacgta atatgggacg cacagcgact ggggtacgag
    11161 gcgttaatct tcgtgaaggt gataaagttg tgggagcttc tcgtatcgtt aatggacaag
    11221 aagtactaat catcactgaa aaaggttatg gcaagagaac agaagcttcg gaatatccta
    11281 caaaaggccg tggtggtaaa ggaataaaaa ccgctaatat tactgctaaa aatgggccat
    11341 tagcacgctt agtgacgatt aatggaaatg aggacatcat ggtcattaca gatacaggtg
    11401 ttattattcg aaccaatgtt gctaatattt ctcaaactgg tcgttctacc atgggtgtta
    11461 aagtgatgag acttgatcaa gaagctaaaa tcgtcactgt tgcgctagtg gaacaagaaa
    11521 ttgaagataa gtctaatata gaggatacaa aagaatagga agttatgcgt aataaaaaaa
    11581 aatcacatgg tttctttaac tttgttagat ggttattggt tgtattgtta attattgttg
    11641 ggttggcttt ggtgttcaat aaaccaattc gcaatgcttt catagctcat caatcaaatc
    11701 attatcaaat ttcacgagtt agtaaaaaaa caattgaaaa aaataagaaa tctaaaacaa
    11761 gttatgactt ttcatcagtc aaatctatta gtacagaatc gattttatct gctcaaacga
    11821 aatcacataa tttgcctgta ataggaggga ttgctatacc agatgtcgaa atcaatctcc
    11881 caattttcaa aggactgggc aatacagagt tgagttatgg tgcaggaacg atgaaagaaa
    11941 accagattat gggaggccca aataactatg ccttagctag tcaccatgtc tttggtttga
    12001 caggttcttc taagatgctc ttttcacctt tagaacatgc taagaaaggt atgaaagttt
    12061 acctcactga taaaagcaaa gtatacactt acactattac tgaaatttca aaggtaacac
    12121 cagaacatgt tgaggttatt gatgatacac ctggcaagag ccaactcact ttagtgacct
    12181 gtacggatcc agaagccaca gaacgtatta ttgtgcatgc tgaattagaa aaaacaggtg
    12241 aattttcaac agcagatgag tcgatattaa aagcattttc gaaaaaatat aatcaaatta
    12301 atctctagaa ggtagttaga attatgaaat taaaggctgt tcatcatatt gcgataattg
    12361 tttctgacta tgaaaagtca aaagacttct atgttaataa gctaggattt gaaattattc
    12421 gtgaaaacca tcgtccggag cgtcatgatt ataaattaga tttacggtgc ggtgatattg
    12481 agcttgaaat atttggaaat cgtcttgatg atccagaata tgaaactcct cctcagagaa
    12541 taggtaggcc gaattggcca cgtgaagcat gtggtttacg acaccttgcc ttttacgttc
    12601 cagatgtgga agcgtacaag gtagaactgg agaatttagg aatttttgta gagcctattc
    12661 gttacgatga ctatactggc aaaaagatga cctttttctt tgatccagat ggtttacctt
    12721 tagaattaca tgaataataa acaaagttgc gtggatacgc aactttgttt aatttactga
    12781 ataattttat attcaagaag ttttttaaag aaataagtaa gttaatcatg gtataatgta
    12841 aggtatgaaa agaattgaaa aggaattatt ttgaaatgag gatactaaaa tgagtattaa
    12901 aaagctattg tttgcaggcc ttgcttttat tttatttacc ctagcgagtc cagcatatgc
    12961 tgcatcagat gttcagaaag ttattgatga aacttatgtt caacctgatt atgtcttagg
    13021 ttattcgcta aatcaagaac agcgtgcaca gacattacaa cttctgaatt atgatgaatc
    13081 tagagacacg aaagttaaaa cgctaaatac tagctcatat gctaaaataa tgaatatagc
    13141 tgatgatgca agcatccaat tatattcatc tgttaaaata aaaaaattag ggtctaacga
    13201 taccttagca gtaaacattg ttacacctga gaatattact aaagttacag aagatatgta
    13261 tcgcaatgct gcagttacat taggtattga gcatgcaacg atatcagtgg ctgctccaat
    13321 aaaagtaact ggggagagtg ctcttgcagg tatctattat tcattagaga aaaatggagc
    13381 aagtgtatca tcagaaaata aacaactagc acaagaagag ttaagtactt tatctggtat
    13441 taatgctgag aataagggta aggaaggtta tgacgccgac aagcttaatg tagctttaac
    13501 ggatattaag tcagccgtag ctaaaggagg atctgatctt tcaaaagatg atattcgaaa
    13561 aattgttgag gaaacattga aaaattatca tcttgataac gctgtaacgg aaaatcaaat
    13621 aaatttaatt gttaattttg cagttaatct ttcacaaagt aatgtcatca aaaatagtga
    13681 tttcacgaac accttgaata atttgaaaga caacattgtt tcgaaagcag gatctaaatt
    13741 taaaaatatc aatgtcaatt ttaatgctaa taaagctgtt gaatcaggta agggattttt
    13801 agctaatatt tggcaacaaa ttgttaattt tttccagcag tttggtagtt aataaaaaag
    13861 tctagcttat aagc
//
LOCUS       DATROQ010000049        13871 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.050, whole genome shotgun sequence.
ACCESSION   DATROQ010000049 DATROQ010000000
VERSION     DATROQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13871
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.050"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            93..798
                     /locus_tag="VBC94_001279"
                     /pseudo
     CDS             93..798
                     /locus_tag="VBC94_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
     gene            1064..2296
                     /gene="arcA"
                     /locus_tag="VBC94_001280"
     CDS             1064..2296
                     /gene="arcA"
                     /locus_tag="VBC94_001280"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEO6979553.1"
                     /translation="MTQTHPIHVFSEIGKLKKVMLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRNEGVEVLYLENLAAESLTNQEIREQFIDEYIGEANVRGRA
                     TKKAIRELLLNIKDNKELIEKTMAGIQKSELPEIPSSEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGNGVSLNHMFSETRNRETLYGKYIFTHHPEYGGKVPMVYDREETTRI
                     EGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHLD
                     TVFTMVDYDKFTIHPEIEGDLRVYSVTYENQDLHIEEEKGDLADLLAKNLGVEKVELI
                     RCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKINGSELV
                     RGRGGPRCMSMPFEREDL"
     gene            2302..2732
                     /locus_tag="VBC94_001281"
                     /pseudo
     CDS             2302..2732
                     /locus_tag="VBC94_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772116.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            2748..3761
                     /gene="argF"
                     /locus_tag="VBC94_001282"
     CDS             2748..3761
                     /gene="argF"
                     /locus_tag="VBC94_001282"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO6979554.1"
                     /translation="MTQVFQGRSFLAEKDFSREEFEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTIKENFGKLEGITLVY
                     CGDGRNNVANSLLVAGTLMGVNVHIFSPKELFPAEEIVKLAEGYAKESGAHVLVTDNV
                     DEAVKGADVFYTDVWVSMGEEDKFKERVELLQPYQVNMELIKKANNDNLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            3824..5251
                     /gene="arcD"
                     /locus_tag="VBC94_001283"
     CDS             3824..5251
                     /gene="arcD"
                     /locus_tag="VBC94_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEO6979555.1"
                     /translation="MEKEKKLGLLPLTMLVIGSLIGGGIFDLMQNMSSRAGLVPMLIA
                     WVITAIGMGTFVLSFQNLSEKRPDLTAGIFSYAKEGFGNFMGFNSAWGYWLSAWLGNV
                     AYAALLFSSLGYFFKFFGNGNNIISIIGASIVIWVVHFLILRGVNTAAFINTVVTFAK
                     LVPVIIFLISALLAFKFNIFSLDIWGNGLHQSIFNQVNSTMKTAVWVFIGIEGAVVFS
                     GRAKKHSDIGKASILALFTMISLYVLISVLSLGIMSRPELANLKTPAMAYVLEKAVGH
                     WGAILVNLGVIISVFGAILAWTLFAAELPYQAAKEGAFPKFFAKENKNKAPINSLLVT
                     NLCVQAFLITFLFTQSAYRFGFALASSAILIPYAFTALYQLQFTLREDKSTPGHQKNL
                     IIGILATIYAIYLIYAGGFDYLLLTMIAYTLGMILYIKMRKDDKLPIFVRYEKISAIV
                     ILALCLLCIIEIMTGQIDIAAILAG"
     gene            5272..6228
                     /gene="arcC"
                     /locus_tag="VBC94_001284"
     CDS             5272..6228
                     /gene="arcC"
                     /locus_tag="VBC94_001284"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO6979556.1"
                     /translation="MVKEHQKIVVALGGNAILSTDASAKAQQEALINTSKSLVKLIKE
                     GHDVIVTHGNGPQVGNLLLQQADSDSEKNPAMPLDTCVAMTEGSIGFWLQNALNNELQ
                     EQGIDKEVATVVTQVIVDEKDQAFTNPTKPIGPFLSEEDAKKQAQETGSKFKEDAGRG
                     WRKVVPSPKPVGIKEASVIRRLVDSGVVVISAGGGGVPVIEDANTKALKGVEAVIDKD
                     FASQTLSELVDADLFIVLTGVDNVFVNFNKPNQEKLEEVTVSQMKQYITENQFAPGSM
                     LPKVEAAIAFVENKPEARAIITSLENIDNVLAENAGTQIVVN"
     gene            complement(6348..7373)
                     /gene="trpS"
                     /locus_tag="VBC94_001285"
     CDS             complement(6348..7373)
                     /gene="trpS"
                     /locus_tag="VBC94_001285"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEO6979557.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQNEGSYTLFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTLFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPAGFLVYPVAQAADITAFKANLVPVGTDQKPMIE
                     QTREIVRSFNHAYNCQVLVEPEGIYPENDAAGRLPGLDGNAKMSKSLNNGIFLADDMD
                     TVKKKVMSMYTDPNHIKVEEPGQIEGNMVFHYLDVFGRDEDQKEITAMKEHYQKGGLG
                     DVKTKRYLLDILERELSPIRERRLEYAKDMGQVYQMLQKGSEKAQAVAASTLDEVKSA
                     MGLNYFK"
     gene            complement(7481..8173)
                     /locus_tag="VBC94_001286"
     CDS             complement(7481..8173)
                     /locus_tag="VBC94_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO6979558.1"
                     /translation="MRRDDIIMPQDGRKMAVLLGFIGGFIDVYSHMQFHSLVATQTGN
                     IILMVANLGQNDFHFFITKLFSIFFFSIGFLVGIVIKERAVTAFWRLYALCPLLLSTM
                     FIPFVENVNHNIWVALLAFSTGILMLTFSGSRIESEPYTILMTSGNYRKMLQFWYEYI
                     VNRQRTAMQKRRAINYSLVVLAFIIGALVAAIVYDIFAYRAILGVTITLLIIMIHYTI
                     EIIKNDLTLHNV"
     gene            8417..9289
                     /locus_tag="VBC94_001287"
     CDS             8417..9289
                     /locus_tag="VBC94_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6979559.1"
                     /translation="MSKKIKNFILLNIGSLIVAFGFNAFFLHNHIVTGGISGLAVSFH
                     EVFGWDSAFFIMIINIPLLLLCYFGLGKQTFLKTVYGSWIFPVFIKLTQSVPTLTHNP
                     LLAALFGGVIVGCGLGIVFWSDSSTGGTGIIIQFLGKYTPISLGQGVILIDGLVTIVG
                     FLAFDSDTVMFSIIGLITISYIINAIQTGFTTLSTVLIVSQEHQKIKTYINTVADRGV
                     TEIPVKGGYSGTNQIMLMTTIAGYEFAKLQEAIAEIDETAFITVTPTSQASGRGFSLQ
                     KNHGRLDEDILMPM"
     gene            9355..10974
                     /locus_tag="VBC94_001288"
     CDS             9355..10974
                     /locus_tag="VBC94_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6979560.1"
                     /translation="MLTVSDVSLRFSDRKLFDEVNINFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHIALGPDERLSVLRQNHFDYEDERVIDVVIMGNETLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNISEELHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKESSELAARLQADRNAKAEEKIKQLQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFVNFKAEREMGNDLLTVENLSVTIDGEKI
                     LDNISFILRPGDKTALIGQNDIQTTALIRALMGDIEYEGTIKWGVTTSRSYLPKDNSR
                     DFASGESILEWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKDFKESIIFASHDHEFIQTLANHIIIL
                     SKNGVIDRIDETYDEFLENTEVQAKVAQLWK"
     gene            11097..13676
                     /locus_tag="VBC94_001289"
     CDS             11097..13676
                     /locus_tag="VBC94_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEO6979561.1"
                     /translation="MTINHYKLRIPYYTISFLLPFIIIVCILFTKNIYWGSPTTILAS
                     DSFHQYVIFNQALRNILHGSNSLFYTFTSGLGLNFYALSSYYLGSFLSPIVYFFNLKN
                     MPDAIYLLTICKVGLIGLSMFVTLCKRHCKVNRVLLLVISTCYSLMSFSISQIEINMW
                     LDVFILIPLVVLGVDQLLWERKPILHFLSLTALFIQNYYFGFMTTIFTSLYFIVQITR
                     NTDSKVAFKQFLHFTFLSLLAGMTSSIMILPTYFDLTTHGEKLTKVSKMFTENSWYMD
                     LFAKNMIGAYDTTKFGSIPMIYVGLLPLLLSLLYFTIKEIPRRTRLAYGFSLIFVIAS
                     FYITPLDLFWQGMHAPNMFLHRYSWVLSVLICLLAAECLEYLDNISWKKILGVNLILI
                     SGFIITFLFKKHYHYLNLELLLLTLTFLSAYIILTISFVSKQIPKLVFYPFLIGFVVL
                     EMTLNTFYQLNSLNDEWVFPSRQGYAKYNHSISKLVRKTERNNSTFFRTERWLGQTGN
                     DSMKYNYNGISQFSSIRNRSSSQVLDRLGFKSDGTNLNLRYQNNTLIADSLFGVKYNL
                     TEYPFDKFGFIRKAQDKQTILYENQFASQLAILTNQVYQDKPFTVNTLDNQTTLLNQL
                     SGLKETYFEHLIPNSVSGQTTLNKQVFVRKNKQGNTEITYNITIPKNSQLYVSMPFIN
                     FNNEENKIVQISVNNGPFVPNTLDNAYSFFNIGSFAENSRIKVKFQFPHNDQVSFPIP
                     HFYGLKLEAYQKAMTVINKRKVKVRTDHNKVIANYTSPNRSSLFFTIPYDRGWKAYQN
                     NKGIKIFKAQKGFMKINIPKGKGKVTLIFIPYGFKFGVGLSITGIVLFTVYYLKFGKN
                     KIG"
     gene            complement(13744..13817)
                     /locus_tag="VBC94_001290"
     tRNA            complement(13744..13817)
                     /locus_tag="VBC94_001290"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13781..13783),aa:Asn,seq:gtt)
BASE COUNT         4560 a         2364 c         2374 g         4573 t
ORIGIN      
        1 ttcgtagaaa gaacgttttt cgttgaattt tgaaaacgct tttgagaaaa tgtaattgtt
       61 ctgatatctt ttaaaaaaat taagaggcct tcatgactaa atttattgtt gattcatcgt
      121 actggaattt gtttccaact tctaaaattg gagtaatctt aatcaaagat tatcacatgg
      181 acaggaacct tgaaacagaa cttaaacagt tactgtctga tagtcattct ttagcaaaaa
      241 aatatttgca agagaaagaa ttcagtcaaa acaaagttat ccaaacttat cgcaaagcat
      301 atcaaacctt taaaactaaa aaaggtgcac gttcaagtat cgaagcatta ttgaagcgaa
      361 ttaattcagg aaacgaaatt actagtataa atcctctagt tgatatctat aatgccgcta
      421 gccttaggtt tggattacct attggtgctg aggatagtga tacatttaga ggtgatttaa
      481 aactaactat taccaatggt ggtgatgaat tttacttgat tggcgaagat tttaacatac
      541 caactctttc aggtgagctt gcctatgttg atgatgttgg agcagtttgc cgctgtttta
      601 attggcgtga tggaaaacgt actatgatta ccgataatac tcagaacgct tttttagtta
      661 tcgaattaat tgataataga tagagaaatt atttttaaag aagcgttgga ctttatcgcc
      721 accaatacaa atcgctttct aaaagctaaa acacagacca tcatattaga taaagaacac
      781 agtgaaataa cgctataaca ttaaaacgaa aagtaaaaat tttacttttc gttatttttt
      841 aggttaataa tttctcactt tccaacgata aaagaaagaa attgtgtaga aatcgctttc
      901 atttttataa atttaaattt ttttggatat aatcgtgaca ttcttaacac ttttaatgcg
      961 aataacttcc ctattttata ttcaaaagaa agtagaatgt aagtgtagat atagagataa
     1021 atcctgtttc aatgcaaatc tctaactaag aaaggagtaa aatatgactc aaacacatcc
     1081 aattcatgtt ttctcagaaa ttggaaaact taaaaaagtt atgctacacc gtccaggtaa
     1141 agaaatagaa aatctgatgc ctgattattt agagagactc ttgtttgatg atatcccatt
     1201 tttggaagat gcacaaaaag aacacgatgc gtttgcacaa gcacttcgca acgaaggagt
     1261 cgaagtactt taccttgaaa atcttgctgc cgagtcgcta acaaaccaag aaattcgtga
     1321 acagtttatc gatgagtaca ttggcgaagc aaatgttcgc ggtcgtgcta ctaaaaaagc
     1381 tattcgagaa ttattactca atatcaaaga taataaagaa cttatcgaaa aaactatggc
     1441 aggtatccaa aagtctgagc tacctgaaat cccatcttct gaaaaaggtc taactgatct
     1501 tgtcgaatct aattatccat ttgccataga tccgatgcca aatctttact ttacacgtga
     1561 tccatttgca actattggga atggagtttc attaaaccat atgttctctg aaacacgtaa
     1621 tcgcgaaact ttatacggta aatatatctt tacacatcac ccagagtacg gtggaaaagt
     1681 ccctatggtc tatgatcgtg aagaaacaac tcgtattgaa ggtggagatg agctcgtcct
     1741 ttctaaagat gtccttgccg tgggtatctc tcaacgtact gatgctgcct ctattgaaaa
     1801 actcttagtc aatattttta aacaaaattt aggttttaaa aaggttttag catttgaatt
     1861 tgcaaataat cgtaaattta tgcaccttga cacagtcttt acaatggttg actatgacaa
     1921 gtttacaatt cacccagaaa ttgaaggcga tcttcgcgtt tactctgtaa cttacgaaaa
     1981 ccaagatctt catattgaag aagaaaaagg tgatttggca gatcttctag cgaaaaacct
     2041 aggagttgag aaagtggaat tgattcgttg cgggggtgac aacttagtcg ctgctggacg
     2101 tgaacaatgg aatgatggtt caaatacatt aactatagca ccaggtgtcg ttgtcgttta
     2161 caacagaaat acaatcacaa atgcaatctt agagtccaaa ggattgaaat taattaagat
     2221 taatggaagt gaattagttc gtgggcgtgg tggaccacgc tgcatgtcaa tgccatttga
     2281 acgtgaagac ctttaaaatt tatgaagcta ttagtcaatc acttgcctta ccaaagagct
     2341 gcaagcctat atattcgctt ttctgttttc gttatcgaaa gaaatataaa aatgaaagaa
     2401 gaattcgatg ataatgacga gcaagatact atttatgctg ttctttatga tggaaacagc
     2461 cagtttcaac aggacgcttt cttcctgaaa ctcaaacaga agctcgtttg actcgaattg
     2521 cgactttaaa aggttatcgt ggtaacggct atggtaccaa aattattata gcactagaaa
     2581 attatgcaaa agaaaatggt tatcactatc taactataca tgctgaattg acagcaaaag
     2641 atttctacca gacacttggg tatcaagcta ctgggaatat ttatatggaa gacggggaag
     2701 cctgtcaaac actcgaaaaa tatttaattt aaaggagaca ttaacatatg acacaagtat
     2761 ttcaaggacg tagtttctta gcagaaaaag atttttctcg tgaggaattt gaatatctta
     2821 ttgatttttc agctcattta aaagacctta aaaaacgtgg tgttcctcat cattatcttg
     2881 aaggtaaaaa tattgctctc ttatttgaaa aaacatctac tcgtactcgc gcagccttta
     2941 caactgcagc aattgaccta ggcgctcatc cggaatacct tggtgcaaat gatattcaac
     3001 ttggtaaaaa agaatcaaca gaagatactg ctaaggtttt aggacgtatg tttgatggta
     3061 ttgaattccg tggttttagc caaagaatgg ttgaagagct tgctgaattt tctggagtac
     3121 ctgtctggaa tggtttaaca gatgaatggc atccaacaca aatgctagct gactacctta
     3181 ctatcaaaga aaacttcggt aaacttgaag gtattactct tgtttactgt ggtgacggac
     3241 gtaacaatgt tgccaactcg cttttagtgg ctgggacttt gatgggggtc aatgtacaca
     3301 tcttttctcc aaaagaactt ttccctgctg aagagattgt taaattggct gaaggatatg
     3361 ccaaagaatc tggggctcac gttctcgtta ctgataatgt agacgaagct gtaaagggag
     3421 cagacgtctt ttacactgat gtctgggtat cgatgggaga agaagataag ttcaaagaac
     3481 gcgttgaact tcttcaacca tatcaagtaa acatggaact gattaaaaaa gctaataatg
     3541 ataatcttat cttcttacac tgcttacctg cattccatga tacaaatacc gtttatggca
     3601 aagacgtcgc tgaaaaattt ggggtcaagg aaatggaagt tactgatgaa gtcttccgta
     3661 gcaaatatgc tcgtcatttc gaccaagctg aaaatcgtat gcacactatt aaagctgtaa
     3721 tggctgcaac ccttggaaat cttttcattc caaaagttta aattaaacga ctatagggag
     3781 ttgacatata gtcaattccc tattttagga aatgaggtat aaaatggaaa aagaaaaaaa
     3841 attaggtctt ttaccactaa caatgcttgt cattggctct cttatcggtg gcggaatctt
     3901 tgatttaatg caaaatatga gttccagagc cggtttggta ccaatgctta ttgcttgggt
     3961 aattactgct atcgggatgg gaactttcgt tttaagtttt caaaatttat ctgaaaaaag
     4021 gccggatcta acagctggaa tctttagtta cgctaaagag gggtttggaa actttatggg
     4081 atttaactct gcatggggtt attggttatc agcttggctt ggaaatgttg cctacgctgc
     4141 actcttattc agttcactcg gttatttctt taaattcttt ggtaatggaa ataatatcat
     4201 ctcaattatt ggagcaagta tagttatttg ggttgtccat ttcttaattt taagaggtgt
     4261 taatacagct gcatttatta ataccgtagt tacctttgca aaattagtac ctgttattat
     4321 tttcttaatt tcagcgttat tagctttcaa atttaacatt tttagtcttg atatctgggg
     4381 aaatggatta catcaatcaa ttttcaacca agtcaattca actatgaaaa ccgctgtttg
     4441 ggtatttatt ggtattgagg gcgccgttgt cttctcaggt cgtgctaaaa aacactctga
     4501 tattggtaaa gcaagtatcc tagcattatt cactatgatt tcactttatg tattgatttc
     4561 tgttttatca cttggtatca tgtcacgtcc agaacttgca aacttaaaaa caccagctat
     4621 ggcttacgtt ctagaaaaag ctgttggtca ctggggtgct atcttagtta accttggtgt
     4681 tatcatttca gtatttggcg ctattcttgc ttggacttta tttgcagcag aattaccata
     4741 tcaagctgct aaagaaggtg cttttcctaa attttttgca aaagaaaata aaaacaaagc
     4801 tccaatcaac tcactcttag tcactaatct ttgtgtacaa gcattcttaa tcacgttctt
     4861 attcacacaa agtgcttatc gttttggttt cgcattagca tcatctgcta tcttaattcc
     4921 ttatgctttt acagcactat atcaattaca attcacactc cgtgaggata agtcaactcc
     4981 aggacatcaa aagaatttaa ttatcggtat cctcgctaca atctatgcta tttaccttat
     5041 ctacgctggt ggttttgatt acttactttt gacaatgatt gcttatactc taggtatgat
     5101 tctctatatt aaaatgagaa aagatgacaa gcttcctatc tttgtgagat atgaaaaaat
     5161 tagtgccatt gtcattctag cgctttgctt actatgtatt attgaaatta tgactggaca
     5221 aattgacatt gctgcaatcc tagcaggtta gtcattagaa agagggataa attggtaaaa
     5281 gaacatcaaa aaatcgtagt tgccttaggt ggtaacgcta ttctatcaac agatgcttca
     5341 gcaaaagcac aacaagaagc attaatcaac acatcaaaat cactggtcaa actgattaaa
     5401 gaaggccatg atgtcattgt tactcatgga aatggtcccc aagttggtaa cttattgcta
     5461 cagcaagcag attcagattc tgaaaagaat ccagcaatgc ctttggatac ttgtgttgcc
     5521 atgacagaag gaagtattgg tttttggtta caaaatgctc ttaacaatga attacaagaa
     5581 caaggcattg acaaagaggt tgcaacagtg gtaactcaag ttattgtaga tgaaaaagat
     5641 caagctttca ctaatccaac aaaacctatt ggcccattct tatctgaaga agatgccaag
     5701 aaacaagccc aagaaacagg ttcaaaattt aaagaagatg ctggtcgtgg atggcgtaaa
     5761 gtcgttcctt caccaaaacc ggttggtatc aaagaagctt ctgttattag acggttagtg
     5821 gactctggtg tggttgttat tagcgcaggt ggcggaggtg taccagttat tgaagatgct
     5881 aatacaaaag cattaaaagg ggttgaagct gttattgaca aagactttgc cagtcaaact
     5941 ttatcagaac ttgttgatgc cgacttattc atcgtcttaa ctggcgttga caacgttttt
     6001 gttaatttta ataaacctaa tcaagaaaaa ttagaggaag ttactgtttc acaaatgaaa
     6061 caatatatca ctgaaaatca atttgcccct ggcagtatgc taccaaaggt agaagctgcg
     6121 attgcttttg ttgaaaataa accagaagcg cgtgctatca ttacttcact tgaaaatatt
     6181 gataatgtct tagcagaaaa tgcagggaca caaattgttg taaattaaac aatgtagatt
     6241 accattacat acactcctta ttaataacaa agtaatgtca ctccttaggc attcctataa
     6301 taaaagagag ctagatatat ctagctctct tttattatag gaatatatta tttaaaatag
     6361 ttcaaaccca tggctgattt aacctcatcc aaagtagagg ctgcaacagc ttgagctttc
     6421 tcactacctt tttgtaacat ttgataaact tgtcccatat ctttagcata ttcaaggcga
     6481 cgttctctaa taggtgataa ttcacgctct aagatatcaa gtagataacg ttttgtttta
     6541 acatctccaa gtccaccttt ttgatagtgt tctttcatag cagtaatctc tttttggtct
     6601 tcatctcttc caaaaacatc aaggtaatgg aataccatat taccttcgat ttgtcctggt
     6661 tcttctactt taatatggtt tggatcagta tacatgctca taactttttt cttaactgta
     6721 tccatatcat ctgctaaaaa gattccatta tttaatgact tagacatttt tgcattacca
     6781 tctaaccctg gcagacgacc tgctgcatca ttttctggat agattccctc tggttcaact
     6841 aaaacctgac aattataagc atgattgaaa gatctaacta tttcacgtgt ttgttcaatc
     6901 attggttttt ggtcagtccc tacaggaaca aggttagctt taaaagcggt tatatcagct
     6961 gcttgtgcaa caggataaac taagaatcct gctggaatag actcaccaaa acctttttga
     7021 gcaatttctg tttttacagt tggattacgt tccaaacgtg caagtgaaac caagttcata
     7081 taatacatac ttaattctgc taactcagga atttgacttt gaatgaataa agtcgacttg
     7141 tttggatcca gcccaacagc taaataatca agtgcaacat taccaattga ttcaacaata
     7201 gtttgcggat ctttagcatg gtctgtcagt gcttgttggt ctgctaaaaa gacaaataaa
     7261 gtatagctac cctcattttg aagtagaact ctatttttta agctacctac ataatgtcca
     7321 atatgcaact ttcctgtcgg tctatcacct gttaagataa ttggttttgt catgtttact
     7381 ccttaatata ccaaagatat catttcatat agttatctta tcatgtcaca agccctattg
     7441 caattcatcc ctacaagaaa cattcacctt attaagatga ttaaacatta tgtaacgtta
     7501 aatcgttctt aataatttca atcgtataat gaatcataat aattaaaagc gttatcgtca
     7561 cacctaaaat agctctatat gcaaagatat cgtaaactat tgcagctacc aatgctccaa
     7621 taataaatgc tagaactacc agtgaataat tgatagctcg tcttttttgc atagccgttc
     7681 tctgacgatt aacaatatac tcataccaaa attgtagcat tttccgataa ttaccagaag
     7741 tcattaagat agtgtatggt tcagattcaa tgcgacttcc tgaaaatgtc aacattaaaa
     7801 tacctgtgct aaaggctaat aaagctaccc agatattgtg gtttacattt tcaacaaaag
     7861 gaataaacat cgtagaaagt aaaaggggac ataatgcata tagacgccaa aaagctgtta
     7921 ctgcacgttc tttaatcaca ataccaacca aaaaaccaat tgaaaaaaag aaaatagaaa
     7981 ataatttagt gataaaaaag tgaaaatcat tttgtccgag atttgccacc atcaagataa
     8041 tattacctgt ctgtgttgca acaagggagt gaaactgcat gtgactataa acgtcaataa
     8101 aaccaccaat aaaacccaat aatacagcca tttttcttcc atcttgtggc attataatat
     8161 catctctgcg cattatagca aattctcctt cttgtattat catgttttct attatatacc
     8221 aataaaatag gcattcaact caaaaatatc gagaaatttc ttttctaaat ttacttgact
     8281 accaagtctc gctgttagat aataggtaat aataagtttt gataaaataa aaatgttatg
     8341 actgagttgt caatattatt tattgacagc tttctctttt tatcatgtta ttattactaa
     8401 agttaaggag cttatcatgt ctaaaaaaat taagaatttt atactattaa atattggatc
     8461 tctaattgtt gcatttggtt tcaacgcttt ctttcttcat aatcatattg tcacgggggg
     8521 gattagtggt ttggcagtta gttttcatga agtatttggt tgggattctg ctttttttat
     8581 tatgattatc aatattccat tgctccttct ttgctacttt ggcttaggta aacaaacctt
     8641 tttaaaaact gtctatggtt cttggatttt tcctgttttt attaagttaa cacaaagtgt
     8701 accaactttg acccacaacc cactcctcgc agcacttttt ggaggtgtta ttgtaggatg
     8761 tggtttgggg attgtttttt ggagcgactc ttcaactggt ggaacgggga ttatcattca
     8821 attcttagga aaatatactc ctataagcct tggacaaggg gttatattga ttgatggact
     8881 tgttacaatt gttggtttcc tagcttttga cagtgatacg gttatgtttt ctattattgg
     8941 gttgataact attagttata ttattaatgc tatccaaact ggatttacaa ccttaagcac
     9001 tgtcttaatc gtttctcaag agcaccaaaa aattaagaca tatatcaata ctgtcgcaga
     9061 tagaggagta acagaaattc ccgttaaagg gggatattct ggaactaatc aaatcatgct
     9121 tatgacaact attgctggtt atgagtttgc taaattacaa gaggcaatag cagaaattga
     9181 cgaaacagcc ttcataacag taactccaac atcacaagct tctggacgtg gatttagtct
     9241 tcaaaaaaat catggacgtc ttgatgaaga cattcttatg ccaatgtaat cgattgataa
     9301 ttaaagcttt ttctagtaga atagcagata gaatatacac aagaggtaac aaaattgctt
     9361 acagtttcag atgtttcact acgttttagt gatcgcaaat tatttgatga agttaacatt
     9421 aactttacag caggaaatac ctatggtctt atcggagcaa atggcgctgg taaatcaact
     9481 ttccttaaaa ttttagctgg tgatattgaa ccaacaacgg gacatattgc acttggccca
     9541 gatgaacgcc tttctgtact tcgccaaaat cattttgatt atgaagatga gcgcgtcatt
     9601 gatgtcgtta tcatgggtaa tgaaacactt tatagtatca tgaaagaaaa ggatgctatc
     9661 tacatgaaag aagatttttc tgatgaagat ggtgttcgcg ctgcggaatt agaaggagaa
     9721 tttgctgaac tcggtggatg ggaggctgaa agtgaggctt ctcaattact ccaaaatcta
     9781 aatatttcag aagagcttca ttatcaaaat atgagtgaat tagctaatgg agacaaagtt
     9841 aaagttctcc ttgcaaaagc tctctttggt aaaccagatg tacttctcct tgacgagcct
     9901 accaatggac ttgatatcca gtctattact tggttagaag atttcttgat tgactttgaa
     9961 aatacagtta ttgtagtttc ccacgaccgt catttcttga ataaagtatg tacacatatg
    10021 gctgaccttg attttggaaa aattaaactg ttcgtcggca actatgattt ctggaaagaa
    10081 tcatcagaat tagcagctcg tttacaagct gatcgtaatg ctaaagcaga agaaaaaatt
    10141 aaacaacttc aagaattcgt tgcacgattt tcagcgaatg cttcaaaatc aaaacaagca
    10201 acttctcgta aaaaaatgct tgataaaatt gaacttgaag agattgtacc atcaagccgt
    10261 aaatacccat tcgttaactt taaagccgaa cgcgaaatgg gaaatgattt attgaccgtt
    10321 gaaaatctct ctgtcactat tgatggggaa aaaatccttg ataatatcag tttcattctt
    10381 cgtcctggtg ataaaactgc acttattgga caaaatgata tccaaactac tgcacttatt
    10441 cgagcattga tgggggatat cgaatatgaa ggaacaatca aatggggagt aacaactagt
    10501 cgttcttacc ttccaaaaga caattcacga gactttgctt ctggcgagtc aatcttagaa
    10561 tggcttcgtc aatttgctag taaagaagaa gacgataata ccttcttacg tggcttccta
    10621 ggtcgtatgc ttttctctgg agatgaggtt aacaaatctg ttaatgtctt atctgggggt
    10681 gaaaaagtac gcgttatgct ttcgaaatta atgctattaa aatctaatgt tcttgttctt
    10741 gatgatccaa caaaccactt agatttggaa tctatttcaa gtctcaatga tggactaaaa
    10801 gatttcaaag aatctattat ttttgcaagt catgaccacg agtttattca aactttagcg
    10861 aaccatatta ttattctttc taaaaacggt gttatcgatc gtatcgatga aacttacgat
    10921 gaatttcttg aaaataccga agtacaagct aaggtcgctc aactctggaa ataatccttt
    10981 ggcatatctc taacctaata aacgtgtcaa gattgaaaat cttaaaacta tattaatcat
    11041 ttaaataaaa aggttggaac aaatagcgtc cagccttttt atcttaggag atatatatga
    11101 caattaacca ctataagctt cgtatacctt actatacaat aagctttcta cttcccttca
    11161 taatcatagt ctgtatcctc tttactaaga atatttattg gggaagtcca acaaccatat
    11221 tagcaagcga tagctttcat caatatgtta tctttaatca agcattaaga aatattcttc
    11281 atggttcaaa cagcttattc tataccttta caagcggttt aggtttaaat ttttatgctc
    11341 tgagtagtta ctatttggga agttttttat ccccaatagt ttatttcttt aacctaaaaa
    11401 atatgcctga tgctatctac cttttaacaa tatgtaaagt agggttgata ggtttgtcaa
    11461 tgtttgtcac tctttgtaaa cgtcattgta aagttaatcg tgtcttactt ttagtaattt
    11521 ctacttgcta tagcttaatg agtttctcaa ttagccaaat tgaaattaat atgtggctag
    11581 atgtatttat tctaattccc cttgttgttt taggagttga tcagctacta tgggaaagaa
    11641 aacctatact tcattttctc tctctgacag ccttattcat ccaaaactat tattttggtt
    11701 ttatgactac catctttact agcctttact tcatcgtaca aataacccgt aatactgata
    11761 gtaaggttgc atttaaacaa tttcttcatt ttaccttttt atctctttta gctggtatga
    11821 ctagtagcat tatgattttg ccaacttatt ttgatttaac tactcatggt gaaaagttga
    11881 caaaggtatc gaagatgttt acagaaaatt cttggtacat ggatttgttt gctaaaaata
    11941 tgattggagc ctatgataca acaaaatttg gctctattcc tatgatttat gtaggtttac
    12001 ttcctttact cctttcttta ctttacttta caataaagga aattcctcga aggactagat
    12061 tagcatatgg attttcacta atatttgtta ttgctagttt ttatataaca cctctagatt
    12121 tattttggca agggatgcat gctccaaata tgtttcttca tcgctactct tgggtacttt
    12181 cagtacttat ctgtctacta gctgctgaat gccttgagta tttagacaac atttcttgga
    12241 aaaagatttt aggggtcaat ttaattttaa taagtggttt cataatcacg tttttattta
    12301 aaaagcacta tcattacctt aatcttgaat tacttctttt aaccttaaca ttcctctcag
    12361 cttatattat tctaactatt agttttgtca gtaagcaaat ccctaaatta gttttttatc
    12421 cctttcttat tggttttgtt gtgttagaga tgacattaaa caccttttac caactaaata
    12481 gtcttaacga tgagtgggta tttccatcta ggcaaggata tgctaaatat aatcacagca
    12541 ttagcaaact tgtcagaaag actgagagaa ataactctac attcttccgt acagagcgct
    12601 ggttaggaca aaccggcaac gactctatga aatataatta taatggtatt tcacaatttt
    12661 catctattag aaatcgttct tcaagtcaag tgcttgatag acttggcttt aaatcagatg
    12721 gtacaaattt aaatttacgt tatcaaaata atactttgat cgcagatagt ctatttggtg
    12781 taaagtacaa ccttacagaa tatccttttg ataaatttgg ttttatcaga aaagctcaag
    12841 ataaacaaac cattctttac gaaaatcagt ttgccagtca attagctata ctaacgaatc
    12901 aagtatatca agataaacca tttactgtga atactttgga taatcagaca actcttttaa
    12961 atcaattaag tggtttgaaa gagacgtatt ttgagcatct tattccaaat agcgtttctg
    13021 ggcaaactac tcttaataaa caagtatttg ttagaaagaa taaacaaggg aatactgaaa
    13081 ttacctataa cataactatc cctaaaaaca gtcagttata tgttagtatg ccgtttataa
    13141 attttaataa tgaggaaaat aaaattgttc aaatatccgt taataatgga ccatttgtac
    13201 ctaatactct tgataatgct tattcatttt ttaatattgg ttcctttgct gaaaacagtc
    13261 gtatcaaagt aaaatttcaa tttccacaca atgatcaagt ttctttccca attccccact
    13321 tttatgggct caagttagaa gcttatcaaa aagctatgac tgttattaat aaacgaaaag
    13381 taaaagtaag aactgatcac aataaagtta ttgctaatta tacaagtcct aatagaagtt
    13441 ctcttttctt cactattccc tatgatagag gttggaaagc ttatcaaaat aataaaggaa
    13501 taaaaatttt caaagctcag aaaggtttta tgaaaataaa tattcctaaa ggaaaaggca
    13561 aagtaacatt aatttttatc ccttacggct ttaaatttgg ggtaggccta tctattactg
    13621 gaatagtatt atttactgtc tattatttaa aatttggaaa aaataaaata ggatgagagt
    13681 actctcatcc tattttatta acaaaaaaac gccgacaaat caacgtttag atgtgtctta
    13741 tcactccggc agcaggactc gaacctgcga catcatgatt aacagtcatg cgctactacc
    13801 aactgagcta tgccggataa ataaaaaact gcaaagcagt tatcaatagt ccgtacggga
    13861 ttcgaacccg t
//
LOCUS       DATROQ010000050        13819 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.051, whole genome shotgun sequence.
ACCESSION   DATROQ010000050 DATROQ010000000
VERSION     DATROQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13819
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.051"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(52..954)
                     /locus_tag="VBC94_001291"
     CDS             complement(52..954)
                     /locus_tag="VBC94_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6979562.1"
                     /translation="MDIFNQLPDSVLQWFAIFISIIIEALPFVLLGTILSGIIEVFIT
                     PDIVNKFLPKNKFLRVLFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSIRFLILRFVGATIVAIALGVMLAFLVDDNILKEDAKPTHFH
                     DYSDKKWYQKIFLALAHAIDEFFDTGRYLVFGTLIASAMQIYLPTRVLTTIGHSPITA
                     ILVMMLLAFILSLCSEADAFIGASLLSTFGIAPVMAFLLIGPMIDIKNLMMMVNSFKT
                     RFIVQFISVSSLIIIIYCLFVGVI"
     gene            complement(960..1088)
                     /locus_tag="VBC94_001292"
     CDS             complement(960..1088)
                     /locus_tag="VBC94_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979563.1"
                     /translation="MAITHKRQDDLESMFASFAKVPKPKKVDSDSKPEQKDDSQKD"
     gene            1221..2264
                     /locus_tag="VBC94_001293"
     CDS             1221..2264
                     /locus_tag="VBC94_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEO6979564.1"
                     /translation="MIELGITTFGETTILEETNQSYSHPERIRQLVAEIELADQVGLD
                     VYGIGEHHREDFAVSAPEIILAAGAVRTNNIRLSSAVTILSSNDPIRVYQQFSTIDAL
                     SNGRAEIMAGRGSFIESFPLFGYDLADYDDLFNEKMDMLLAINSATNLDWKGHLTQTV
                     NERPIYPRALQRQLSIWVATGGNVDSTIRIAEQGLPIVYATIGGNPKAFRQLVHIYKE
                     VGSRNGHKPEQLKVAAHSWGWIEEDNQTAIDRYFFPTKQTVDNIAKGRPHWSEMTKEQ
                     YLRSVGPEGAIFVGSPEVVAHKIIGLVEALELDRFMLHLPVGSMPHKDVLNAIKLYGE
                     EVAPIVRKYFEKN"
     gene            2368..4530
                     /locus_tag="VBC94_001294"
     CDS             2368..4530
                     /locus_tag="VBC94_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEO6979565.1"
                     /translation="MENLNITQIAIDLGIKASQIEKVLELTDEGNTIPFIARYRKEMT
                     GNLDEVQIKSIIDLDKSMTALSDRKTTVLAKIEEQGKLTQELKKAIEEATKLADVEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNKDNLEEEAQNYLTDGFETTTKALSGAVD
                     ILIEAFSEDNKLRSWTYNEIWNYSSITAVVKDESLDEKQVFKIYYDFSEKISKLHGYQ
                     VLALNRGEKMGVLKVNFEHNLEKMFRFFAVRFKETSQYIDDLIVQTVKKKIVPAMERR
                     IRTELSEGAEDGAISLFSENLRNLLLVSPLKGKMVLGFDPAFRTGAKLAVVDQTGKLM
                     TTQVIYPVPPANQAKIEQSKIELAKLIKEFNIEIIAIGNGTASRESEAFVAEVLQDFP
                     DVSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLAENLDFVVETVVNQVGVNVNTASPALLAHVSGLNKTISENIVKYR
                     EENGQIKSRAEIKKVPRLGAKAFEQAAGFLRIPNAKNFLDNTGVHPESYEAVKKLLDQ
                     LTIKELDDLAKEKLQHLDLIATAESIGVGQETLKDIIEDLLKPGRDLRDDFEAPVLRH
                     DVLDVSDLKVGQELQGTVRNVVDFGAFVDIGVHEDGLIHQSRLIKRKRDKKTRKMPPL
                     PHPSKYLSVGDIVTVWVVEVDAERGRIGLSLIKPDGLD"
     gene            4493..4951
                     /locus_tag="VBC94_001295"
     CDS             4493..4951
                     /locus_tag="VBC94_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEO6979566.1"
                     /translation="MDLVLSSQTDLTNYVKKVSIEDFGCQFLHTAHWNNRLRTTGGRF
                     FPNDRHLDFNPKIYREFGIDIFRKIVRHELCHYHLYIQGKGYRHRDKAFKELLEKVDG
                     LRYTPRVTAVKVNYHHYSCQSCGQVYRRRRRVNLRKFACGYCHGRLIESF"
     gene            5032..5295
                     /locus_tag="VBC94_001296"
     CDS             5032..5295
                     /locus_tag="VBC94_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEO6979567.1"
                     /translation="MKSSFYKQRKGKLVCGVVAGLADKYNWDLALSRVLIALILYFTK
                     FGLLLYILLAVFLPYKEDIIETRRQGPRRRKDAEPVDDDGWFW"
     gene            5523..6458
                     /locus_tag="VBC94_001297"
     CDS             5523..6458
                     /locus_tag="VBC94_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEO6979568.1"
                     /translation="MAVTVQMLVDRLKLNVIYGDEHLLSKRITTADISRPGLEMTGYF
                     DYYAPERLQLVGMKEWSYLMAMTGHNRYQVLREMFQKETPAIVVARDLEIPEEMYEAA
                     KDTGIAILQSKAPTSRLSGEVSWYLDSCLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDIMSLYGAS
                     AVKDSSQVQLAIYLENFETGKVFDRLGNGNEEIELSGVKVPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKQMGFDATQTFEDRLTHLISQNEVNDD"
     gene            6451..7224
                     /locus_tag="VBC94_001298"
     CDS             6451..7224
                     /locus_tag="VBC94_001298"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEO6979569.1"
                     /translation="MINPVAIQLGPFSIRWYAICIVSGMLLAVYLAMKEAPRKNIKSD
                     DILDFILMAFPLSIVGARIYYVIFEWAYYSKHPVEIIAIWNGGIAIYGGLITGAILLV
                     IFSYRRLINPIDFLDIAAPGVMIAQAIGRWGNFINQEAYGRAVKNLNYLPNFIKNQMY
                     IDGAYRVPTFLYESLWNFLGFVIIMSIRHRPRTLKQGEVACFYLVWYGCGRFIIEGMR
                     TDSLYLAGLRVSQWLSVILVIIGIVMIIYHRREQHISYY"
     gene            7239..7637
                     /locus_tag="VBC94_001299"
     CDS             7239..7637
                     /locus_tag="VBC94_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEO6979570.1"
                     /translation="MIEIAVLIIAIAFVVLVLGILFVLKKVSETIEETKQTIKVLTSD
                     VNVTLYQTNEILAKANVLVDDVNGKVSTIDPLFVAIADLSESVSDLNLQARHIGQKAS
                     SATSSVTKAGSALAIGKAASKIFRKKGDKK"
     gene            7634..8065
                     /locus_tag="VBC94_001300"
     CDS             7634..8065
                     /locus_tag="VBC94_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO6979571.1"
                     /translation="MSKLFKTLVISAASGAAAAYFLTTKKGKELRKNAEKFYGEYKEN
                     PEEYHQIAKDKASEYSNLAVDTFKDYKGKFESGELTTEDIVSAVKEKSGEVVDFANDF
                     VNQAKSKFSDEDTAKKEDKAPETKVEDIVIDYKENTEDKEK"
     gene            complement(8106..8381)
                     /locus_tag="VBC94_001301"
     CDS             complement(8106..8381)
                     /locus_tag="VBC94_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEO6979572.1"
                     /translation="MSSPLKQEQTHESKTEQAQPKFQVFQEFDHSAKLRELVFFARIA
                     LFSITTVLISFLLLALSLTPVWSFLLAMLASLAVTSAVSSFIWSLRQ"
     gene            8724..9650
                     /locus_tag="VBC94_001302"
     CDS             8724..9650
                     /locus_tag="VBC94_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO6979573.1"
                     /translation="MEKIILTATAESIEQVKQLLAIGIDRIYVGEENYGLRLPHSFSD
                     DELREIAKLVHDAGKELTVACNALMHQEMMDNIKPFLELMKEINVDYLVVGDAGVFYI
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVETVLAREIPSEELFKMSENLEFPAE
                     ILVYGASVIHHSKRPLLQNYYNFTHITDEKTRERGLFLAEPGDPESHYSIYEDKHGTH
                     IFINNDINMMTKVTELVEHHFTHWKLDGIYCPGDNFVAIAEIFVETARLIENGTFTQD
                     QAFLFDERIRKLHPKGRGLDTGFYDFDPSTVK"
     gene            9780..11066
                     /locus_tag="VBC94_001303"
     CDS             9780..11066
                     /locus_tag="VBC94_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO6979574.1"
                     /translation="MSNVKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGINYAHARDAKVYVAANMVTHEGNELGAGPWFRELRDMGLDAVIVSDP
                     ALIVICATEAPGLEIHLSTQASSTNYETFEFWKEMGLTRVVLAREVTMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGQERQSL
                     KGEIPEPFSMSAVDMCMIEHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFEAIKEDLIDELWKVAQRELATGFYYHTPTENEQLFGARRKIPQYKFVGEVV
                     SFDNAKMEATIRQRNVIMEGDRVEFYGPGFRHFECFIEGLRDAEGNKIDRAPNPMELL
                     TITLPNPVKKGDMIRACKEGLVNLYQNDGTSKTIRA"
     gene            11193..11405
                     /locus_tag="VBC94_001304"
     CDS             11193..11405
                     /locus_tag="VBC94_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEO6979575.1"
                     /translation="MSEFTFEIVEKLLVLSENEKGWTKELNRVSFNGAPAKFDLRTWS
                     PDHTKMGKGITLSNEEFKVILDAFRK"
     gene            11529..12326
                     /locus_tag="VBC94_001305"
     CDS             11529..12326
                     /locus_tag="VBC94_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979576.1"
                     /translation="MIRHSFKSKMLLLVFILTIIVNYLSATGLLTGNSQKSLSDRYQT
                     LLTPAPLAFSIWSVIYLLTFLVILRAIFSKSQSYQDNFASIFPYFLGLLLVNNIWTVF
                     FTSNLIGLSTIIIFAYCILLVIIIKILSKNKSKLLLRITFGIHAGWLLVASLVNLAVY
                     LVKIDFNYPLPKVYIAIIALIFITVLSLYLARVLQNAYLILSVFWAWLMVFKAHLEGN
                     FQHLYFSFQILSLIAAIILGVSFFIIGYYNVKERYKQKRTSPYSRVL"
     gene            complement(12428..13768)
                     /locus_tag="VBC94_001306"
     CDS             complement(12428..13768)
                     /locus_tag="VBC94_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001073016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEO6979577.1"
                     /translation="MNQNHSVKRSLSEVNQSVEVPHNSSFWNTLRAFLGPGALVAVGY
                     MDPGNWITSVIGGATYRYLLLFVVLVSSLMAMQLQQMAGKLGIVTRQDLAQATASRLP
                     KPLRYLLFIIIELALIATDLAEVIGSAIALHLLFGWPLLLSIMITILDVFLLLLLMKL
                     GVQKIEAFVSVLILTILIIFTYLVVLSQPDLDAMFKGFLPHHELFNISHEGKNSPLTL
                     ALGIIGATVMPHNLYLHSSLSQTRRVDYHNKSSIKKAVRFMTLDSNIQLSLAFVVNSL
                     LLVLGASLFYGHANDISAFSQMYLALSDKTITGAVASSFLSTLFAVALLASGQNSTIT
                     GTLTGQIVMEGFLHFKLPQWLIRLCTRLLTLLPIFAIALLVGGEENTLDQLIVYSQVF
                     LSLALPFSIFPLIYFTSQKSIMGEHANTKWNTYLAYLIAIILTLLNLKLIMDLL"
BASE COUNT         4642 a         2108 c         2748 g         4321 t
ORIGIN      
        1 ttaaatacat gcttaattca aaataaccag caagaataag aaatcgaatc attatattac
       61 ccccacaaat aaacaataga taataataat aagagaagat acactaatga attggacaat
      121 aaaccttgtt ttaaaagaat ttaccatcat cattaaattc ttaatatcaa tcatgggtcc
      181 aattaaaagg aaagccatca ctggcgcgat tccaaaggtt gataataaag aagcacctat
      241 aaaagcgtca gcttcactac acaatgacaa gatgaaagca agcaacatca taactaagat
      301 ggctgtgata gggctgtgtc cgatagtagt aagaactcga gttggtaaat aaatttgcat
      361 agctgaagca attaaagtgc caaatactag atagcgtcct gtgtcaaaaa attcatcgat
      421 ggcatgagct aaggctagaa agattttttg ataccacttc ttatcagaat aatcatgaaa
      481 atgagtaggt ttagcgtctt cttttagaat attatcatct actaaaaagg caagcatgac
      541 tcctaaagca atggctacaa tggtagcacc tacaaagcgt aatatcagga agcgtataga
      601 atttccaaat gctgagtagg tcgcaaataa aacaatagga ttaataatag gagcagtagc
      661 aagaaacgga actgccgtgt atgaaggcac tttcttttca aggaatcgat taataatagg
      721 aatgatacca cattcgcaag aaggaaagac aaagccaacg aaggttccaa ataagactct
      781 gagaaattta tttttcggta agaacttatt gactatatca ggtgttataa agacttcaat
      841 aattcctgat aaaatagttc caagcaaaac aaaaggaagt gcctcaataa taatggaaat
      901 gaaaatggca aaccattgaa ggacactatc cggtaattga ttaaagatat ccatagaact
      961 taatctttct gagaatcatc tttctgttca ggttttgaat cagaatcaac ttttttaggt
     1021 tttggtactt tagcaaaact agcgaacata ctttctaagt catcttgacg tttgtgcgta
     1081 atagccatta atggcacctc tttctttata taataaccta ttatactata ttagcttatt
     1141 tttaacataa aaaaacaaat cgattggaaa tgatactata tagtgataaa atgttattag
     1201 tagattgatg aggtaagatt atgattgaat taggtattac aacattcgga gagacaacaa
     1261 tccttgaaga aacaaaccaa agctattcac atcctgagag gattcgccaa ttagttgctg
     1321 agattgaact agctgatcaa gttggtttag atgtatatgg tattggagag caccatcgtg
     1381 aagattttgc ggtctctgca cccgaaatta tcctagcagc aggagcggtt agaactaata
     1441 atatccgttt atctagtgca gtaacgattc tctcttccaa tgatcctatt cgcgtctatc
     1501 agcaattttc aacgattgac gcactttcaa atggtagagc agaaattatg gcagggcgtg
     1561 gttcctttat tgagtctttt ccattgtttg gatacgattt agcggattat gatgatttat
     1621 ttaatgaaaa aatggatatg ttgttagcaa ttaactcagc gacaaatctc gattggaaag
     1681 gtcatttgac acaaacagtt aatgagcgac caatttatcc aagagcatta caaagacagt
     1741 tatcaatatg ggtggcaaca ggaggaaatg ttgattctac aattcgtatt gcagaacaag
     1801 gtttgccaat tgtttatgca actattggtg ggaatcccaa agcctttcgt caattggtcc
     1861 atatttataa agaagttggt agtcgtaatg gacacaaacc agaacaacta aaagttgctg
     1921 ctcactcttg gggatggatt gaagaggata atcaaaccgc tattgaccgt tattttttcc
     1981 ctacgaaaca gaccgtcgat aatattgcta agggacgccc tcattggtct gaaatgacta
     2041 aagagcagta tttacgttca gtaggtccag aaggtgctat ttttgtagga agtcctgaag
     2101 tggttgcaca taaaattata ggacttgttg aagcgcttga gttagaccgt tttatgttac
     2161 atctaccagt tggctctatg cctcacaaag acgtcctaaa tgccatcaaa ctttatggtg
     2221 aggaagtagc accaattgtt cgaaaatatt ttgaaaagaa ttaaagcaga cctggtctgt
     2281 tttttattgt tagattataa aaacgctatc ttgaaatcgc tttttaaaac tcttcgtaag
     2341 tcaaaaatat gctataataa aagcgttatg gaaaatttaa atattacaca aattgctatt
     2401 gatttaggta ttaaagcgtc acaaatcgaa aaggttttag aattaacaga tgaaggaaat
     2461 actattcctt ttattgcccg ttatcgtaaa gaaatgacag gtaatttgga tgaagttcaa
     2521 atcaaatcaa ttattgacct tgataagagt atgacagctc tttcagatcg taaaactacg
     2581 gtcttagcca aaattgaaga acaaggaaag ttgactcaag aactcaaaaa ggctattgaa
     2641 gaagcaacta aactagcaga tgttgaggaa ctctacctac cttataaaga aaaacgtcgt
     2701 acgaaagcta cgattgcacg tgaagcaggt ctttttcctt tagcacgtct tattttacaa
     2761 aataaggata accttgagga agaagctcaa aactatctta cagatggttt tgagacaact
     2821 acgaaggccc tttctggagc agtagatatt cttatcgaag ctttttctga ggataataaa
     2881 ttacgttcat ggacatataa tgagatctgg aactacagct caatcactgc agttgttaaa
     2941 gatgagtcac ttgatgaaaa acaggtcttc aaaatctact atgacttttc tgaaaaaata
     3001 agtaaactcc atggctatca agttttagca ttgaatcgtg gtgagaaaat gggtgttctt
     3061 aaggttaatt ttgagcataa ccttgaaaaa atgtttcgct tttttgctgt tcgatttaaa
     3121 gaaactagcc aatatataga tgaccttatt gtacagactg tcaagaaaaa aattgttcca
     3181 gctatggaac gccgtatccg tacagaacta agtgaaggag cagaagatgg tgcaatttca
     3241 ctgttttcag agaatcttcg taacttattg ttagtctcac cgcttaaggg gaaaatggtg
     3301 ttaggttttg accctgcctt tcgtacagga gcaaaattag cagtagtaga ccaaactgga
     3361 aaattaatga caacacaagt gatttatcca gttcctccag ctaatcaagc aaaaatcgaa
     3421 caatcaaaaa tagagttagc aaaacttatc aaagaattca atatagaaat tattgctata
     3481 gggaatggaa cagcgagtcg tgaatcagaa gcatttgttg cagaagtttt acaagatttt
     3541 ccagacgtat cctatgtgat tgttaacgaa agtggtgcct ctgtttattc agcctcagaa
     3601 ttggcacgtc atgaatttcc agacttaaca gttgaaaaac gttcggctat ctcaattgca
     3661 agacgtctgc aagacccgtt agcagaacta gtcaaaattg atccaaaatc tattggtgtg
     3721 ggtcaatatc aacatgatgt tagtcaaaaa aaattagcag aaaatcttga ttttgttgtt
     3781 gaaactgtag ttaaccaagt aggtgttaat gttaatacag caagtccagc cttgttagct
     3841 cacgtttcag gtttaaataa aacaatatct gaaaatattg ttaaatatcg tgaagagaat
     3901 gggcaaatta agtcacgtgc tgaaattaag aaagtaccca gattaggagc aaaagctttt
     3961 gagcaagctg ctggattctt acgtattcct aatgctaaaa atttcttgga taatacaggc
     4021 gttcaccctg agtcttatga agctgttaaa aagttgcttg accaactgac tatcaaagaa
     4081 ttagatgatc ttgcaaaaga gaagttacaa caccttgact taattgcaac agcagaaagt
     4141 attggggtag gtcaagaaac attaaaagac attatagaag atcttttgaa accaggtcgt
     4201 gatttgcgtg atgattttga agcgcctgtt ttgagacatg atgttttaga tgtcagtgat
     4261 ttaaaagttg gacaggaatt gcaaggaact gttcgaaacg tcgttgattt tggtgctttt
     4321 gttgatattg gtgttcatga agatggtcta atccaccagt ctcgccttat taagcgaaaa
     4381 cgcgataaaa aaactagaaa aatgcctcct ttaccacatc caagcaaata tctctctgtt
     4441 ggtgatattg taactgtttg ggttgtagag gtagatgcgg agcgtggcag aattggactt
     4501 agtcttatca agccagacgg acttgactaa ttatgttaag aaggtgtcta tagaagattt
     4561 tggatgtcag tttttacata ctgcacattg gaataatcgc ttaagaacaa ccggaggacg
     4621 cttctttcca aacgatagac accttgactt taatcccaaa atttatcgtg agtttggtat
     4681 tgatattttt cgtaagattg taagacatga gctttgccat tatcatctct atatacaagg
     4741 taaaggatat cgacataggg ataaagcttt taaagagtta ctagaaaaag tcgatggctt
     4801 acgatatacc cctagagtta cggctgttaa agtgaattat catcattact cttgccaatc
     4861 ttgtggtcaa gtataccgac gtaggagacg tgttaattta cgcaagtttg cttgtggtta
     4921 ttgtcatggt cgcttgatag agagttttta aaatcagtcg taaggcctat ttaattttac
     4981 tgtcaaagta gttataatta aaatatagag tagatagaaa gaaggatagg tatgaaatca
     5041 agcttctaca aacaacgaaa aggtaagttg gtctgcggtg ttgtcgccgg tctagctgat
     5101 aagtataact gggatttagc tctttcacgg gtgttgattg cactgatttt gtattttacc
     5161 aaatttggtc ttttgctata tattttatta gctgttttct taccctataa agaagatatt
     5221 attgaaacaa gaagacaagg tcccagaaga cgtaaagatg ctgaacctgt tgatgatgac
     5281 ggttggttct ggtaattcat ataaaggaga tttgctagac gaatctcctt ttttcttagc
     5341 tttatttaga ccattttttc aaaatatctt ctttttttca gaaaaacgcg ttagcgctta
     5401 cttttagtaa aaagtatgat ataatgtggg tgtaaattaa tgaaagtgtt tagtaccttt
     5461 gctcaacttt aaattgtagg gtatagcaat agggatattc acttttataa gaaggagtaa
     5521 gtatggcagt tactgttcaa atgttagttg accgcttaaa gttaaatgtc atctatggtg
     5581 atgaacatct attatctaag cggattacaa cagctgacat ttcacgtcct ggtctagaaa
     5641 tgacagggta ttttgattat tatgcaccag agcgtttgca acttgttggt atgaaagagt
     5701 ggtcatattt aatggctatg acaggacata atcgttatca agtgttacgt gagatgttcc
     5761 aaaaagaaac accagctatt gttgtagcac gtgatttaga aattccagaa gagatgtatg
     5821 aggcggctaa agataccggt attgctattc ttcaaagtaa ggcccctaca agtcgtttat
     5881 caggtgaagt ttcttggtat ttagattctt gcttagctga gaggacaagt gttcatggtg
     5941 tcttgatgga tatctatggt atgggtgttc tgattcaagg agattccggt attggtaaga
     6001 gtgagacagg actagagcta gttaagcgag gacatcgttt ggtagcagat gaccgtgttg
     6061 atgtttatgc taaagacgaa gagacgcttt ggggagagcc tgctgagata ttacgtcatt
     6121 tacttgaaat tagaggtgtg ggaatcattg atattatgag cctatatgga gctagtgcgg
     6181 ttaaagattc atcgcaagtg caattagcta tataccttga aaactttgaa actggaaaag
     6241 tttttgatag actcggaaat ggcaatgaag agatcgaatt atctggagtt aaggttcctc
     6301 ggatacgtat tcctgttaaa acaggccgaa acgtgtcagt agttattgaa gcagcggcta
     6361 tgaatcacag agctaaacaa atggggtttg atgctaccca gacttttgag gatcgtttaa
     6421 cacatttgat tagtcagaac gaggtcaatg atgattaatc cagtagcaat acaattaggc
     6481 cccttttcta ttcgctggta tgctatttgc atagtgtctg gtatgttgtt agcagtttac
     6541 ttagcaatga aagaggcccc tcgtaagaac attaagtcgg atgatattct agatttcatt
     6601 ctcatggctt ttccactatc cattgttgga gctcgtattt attatgttat cttcgaatgg
     6661 gcttattact ctaagcaccc agtagagatt atcgccattt ggaacggtgg tattgctatt
     6721 tacggagggt taattaccgg agccattctc cttgtgattt tttcatatcg tcgcttgatt
     6781 aatcccattg attttctgga tattgctgct ccaggagtga tgattgccca agccataggt
     6841 agatggggga attttattaa tcaagaagca tacggtaggg cagtaaaaaa tctgaattac
     6901 ttaccaaact ttataaaaaa tcaaatgtat attgatggag cttaccgagt acctacattt
     6961 ttatatgagt ctctttggaa cttcctaggt tttgtcatca tcatgtctat tcgtcatcga
     7021 ccgcgcactt taaaacaagg tgaagtggca tgtttttatt tagtttggta tggatgtgga
     7081 cggttcatta ttgaagggat gaggacagat agcctttatt tggcgggttt gcgcgtgtca
     7141 caatggttat cagttatttt agttattatc ggaattgtca tgattattta tcatcgtcgt
     7201 gaacaacata tttcatatta ttaaacagag gaggcattat gatagaaatt gctgtattaa
     7261 ttattgccat tgcgtttgtt gttttagttt taggaatact tttcgttctg aaaaaagtat
     7321 cagaaacgat tgaggaaacc aagcaaacta ttaaagtatt aacaagcgat gttaacgtga
     7381 ctctttacca aactaatgaa attttagcca aagctaatgt tttagttgat gatgttaatg
     7441 gtaaagtatc tactattgat cctctttttg tagctattgc tgacttatca gaatctgtat
     7501 ctgatttaaa cctacaggca agacacattg gacaaaaggc aagtagtgca acctcaagtg
     7561 ttactaaggc agggtcagct ttagctattg gaaaagcggc ttcaaaaatt tttcgtaaaa
     7621 aaggagacaa aaaatgagta aattatttaa aactttagtt atcagtgctg catcaggtgc
     7681 agcagctgct tattttttaa caacaaaaaa aggaaaagag ctaaggaaaa atgcagaaaa
     7741 attctatgga gaatataaag aaaatccaga agaatatcat caaatagcta aagataaagc
     7801 aagtgaatat tcaaatttag ctgttgatac ttttaaagat tataaaggta aatttgaatc
     7861 aggtgaattg acaacagagg atatcgtctc agccgttaag gaaaaaagcg gagaagtagt
     7921 tgactttgct aatgattttg tcaatcaagc taaatcaaaa ttctcagacg aggatactgc
     7981 taaaaaagaa gataaggctc ctgaaacaaa agtagaagat attgtcattg attataaaga
     8041 aaacacagaa gataaagaaa aataaaaaag aagctactat aagtagcttc ttttttatga
     8101 gatttttatt gtctgagtga ccaaatgaat gaagagactg cagatgtaac tgctagactt
     8161 gccagcatcg ctagtaagaa actccacaca ggtgttaaac ttagtgctag taataagaaa
     8221 gagattagca cagtggttat actgaagaga gcgatacgag caaaaaagac tagctcacgc
     8281 aacttagcac tgtgatcaaa ttcttgaaat acttgaaatt ttggttgagc ttgctctgtt
     8341 ttgctttcgt gagtttgctc ttgcttaagt ggtgatgaca tagctttctc ctttgatact
     8401 agcatatcat atcacaaata atagagtttg tcactagttt tttgatacta tgagttagaa
     8461 taaaggtttt ttctcaaaaa aacaaaaaca ttgcccatat tagtagtaga atactaaaag
     8521 tattttttac tactttcctt acttttttaa attggaaagg aaaaaaatag atataatttc
     8581 gtatagtatt tcaatactat gtttttttct tatctgatag tgcttaacct ctaaagtgat
     8641 ataattttcg aaaagaatat tttttattta ttcttgtcaa gaaatatgct taaaaatgct
     8701 gaaagtttgt tataatacta attatggaaa aaataatttt gacagcgaca gctgaatcta
     8761 ttgaacaagt aaagcaattg ctagcaatag gaattgaccg catctacgta ggggaagaaa
     8821 attacggtct tcgtttacca cattcatttt cagacgatga attgcgtgaa atcgctaaat
     8881 tggtacatga tgctggtaaa gaattgacag tagcttgtaa tgcactcatg catcaagaaa
     8941 tgatggataa tatcaaacca tttttggagt tgatgaaaga aattaatgtg gattatttgg
     9001 tagttggtga tgcaggagta ttttatatca acaagcgtga tggttataac ttcaaactta
     9061 tctacgacac ttctgtattc gttacatcaa gtcgtcaagt taatttttgg ggacaacatg
     9121 gagcagtaga aacggtttta gcacgtgaaa taccttctga agaacttttt aagatgtctg
     9181 agaaccttga atttcctgct gagattttag tttatggggc ttctgtaatt catcactcta
     9241 agcgtccgtt attgcaaaac tattataatt ttactcatat cactgatgaa aaaacacgtg
     9301 agcgtggtct ttttttagca gaaccaggtg atccagaaag tcactattct atttacgaag
     9361 ataagcatgg gactcatatt tttattaaca atgatatcaa tatgatgact aaagtgactg
     9421 aactagttga acatcacttt actcactgga aattagatgg tatttattgt ccaggagata
     9481 attttgttgc tattgctgaa atttttgtcg aaacagctcg tttgattgaa aatggaacat
     9541 tcactcagga tcaggctttc ttgtttgatg aacgaattag aaaactgcac cctaaaggac
     9601 gtggtttaga tactggattt tatgattttg acccatcaac agtaaaataa gatttatttg
     9661 ctacgtgaaa gttttttatc tcaaagcgta gccaattgca ctaagaactg catatcgatt
     9721 ggtatgttaa ttaagataga aaataattct tttatcttga aaatagaagg aaatataaga
     9781 tgtctaatgt aaaaaaacgc cctgaggttt tatcacctgc aggaacacta gaaaaattaa
     9841 aagttgctat tgattatgga gcagatgctg tatttgttgg aggtcaagcg tatggtcttc
     9901 gaagtagagc tggtaacttt tctatggaag agttacaaga gggaataaac tatgctcatg
     9961 cgagagatgc taaagtttat gtagcggcta atatggttac tcatgaaggt aatgagcttg
    10021 gggcaggtcc gtggtttcgt gaattacgtg atatgggact agatgcagtc attgtttcag
    10081 atcctgctct tattgttatt tgtgctacag aagcaccagg tttagaaatt catttgtcaa
    10141 ctcaagcctc ttccacgaac tatgaaactt ttgaattttg gaaagagatg gggcttactc
    10201 gtgtcgtatt agcacgtgag gttactatgg cagagttggc tgaaatcagg aagaggacag
    10261 atgttgagat agaagcattt gttcatggcg cgatgtgtat ttcatactca ggacgatgtg
    10321 ttctatcaaa ccatatgagc catcgtgatg ctaatcgtgg cggttgctct cagtcatgtc
    10381 gttggaaata tgacctctac gatatgccat ttggacaaga acgtcaatcg ttaaaaggcg
    10441 agattccaga acctttctca atgtcagctg tggatatgtg tatgattgag catattccag
    10501 atatgattga aaatggcgta gatagtttaa aaatagaagg acgtatgaaa tccattcatt
    10561 atgtttctac agtaactaat tgctataaag ctgctgtaga tgcctatatg gaaagtccag
    10621 aagcttttga agctattaaa gaagacttga ttgatgaact ttggaaggtt gcacaacgcg
    10681 aattagcaac aggtttttac taccatacac caactgaaaa cgaacaactc tttggagctc
    10741 gtcgtaaaat tcctcaatac aaatttgttg gggaagtggt ttcatttgac aatgctaaaa
    10801 tggaggctac aattcgtcag cgtaatgtta ttatggaagg agatcgcgta gaattctatg
    10861 gtcctggctt ccgtcacttt gaatgtttta ttgaaggtct gcgcgatgct gaaggaaata
    10921 aaatagaccg tgctccaaat ccgatggaat tattaaccat aacattacca aatccagtaa
    10981 aaaaagggga tatgattcgt gcttgtaaag aaggattagt gaacctttat caaaatgatg
    11041 gtactagcaa gactataaga gcttagatac ttttataact attttgtcat tttacatggt
    11101 caatattact aaaaattgtt aaaatagaag aagttcattt aaatagaaca aaaagctata
    11161 aaaataacga atagtataag tggaggtata aaatgtcaga atttacattt gaaattgttg
    11221 agaagttatt ggtcctatct gaaaatgaaa aaggttggac aaaagagttg aatcgtgtta
    11281 gtttcaacgg agcgccagct aaatttgatt tgcgtacatg gagtccagat catacaaaaa
    11341 tgggtaaagg tatcacttta tctaatgaag aatttaaagt cattttagat gcttttcgta
    11401 aataaataaa aagtggtttc aaaaaccgct ttttttgtcg agacaagcga gtattcagta
    11461 aacatgttga ttaaaaaaat agaattttta aactaaaaga attataatgg aggtaaaagg
    11521 agtcctctat gattagacat tcatttaaaa gtaagatgtt attgttagtc tttatcttaa
    11581 caataatagt gaattattta tccgcaacag gtttgttaac agggaacagt caaaagagtc
    11641 tgtctgatcg ttatcaaacg ctcttgacac cagcaccatt agccttttca atttggtcag
    11701 tcatctattt attgaccttt ttagtaatat taagagctat attcagtaag agtcaatctt
    11761 accaagataa ctttgctagc atttttccat actttttggg tcttctatta gttaataata
    11821 tatggactgt attttttacg agcaatttga ttggcctatc aacaataata atttttgctt
    11881 actgtattct tctggttatc attatcaaga tactaagtaa aaataaatca aaattactgc
    11941 ttagaataac atttggaatt catgcaggat ggttgctagt tgcgtcattg gtcaatttag
    12001 cggtctactt agttaaaatt gattttaatt acccattacc aaaggtatat atagctatca
    12061 ttgccttgat ttttataact gttttaagtc tttacctggc tagggtatta caaaatgctt
    12121 acttaatctt atccgttttt tgggcttggt tgatggtttt taaagcacat ttagaaggaa
    12181 attttcaaca cctatatttt agtttccaaa ttttatcttt gattgcagca attatccttg
    12241 gcgtatcgtt tttcatcatc ggttattata atgtcaagga gaggtacaag caaaaacgta
    12301 ctagtcctta ctcacgggtt ctttaaaaga aatacaaaaa gctccatcat tatgtacttt
    12361 cctctatcat tagatgtatg tctcatgata gaggttcagt tcattagtgg ggcttttttg
    12421 tattgtatta aagtaaatcc atgattaatt tcaaattgag taatgttaaa atgatagcga
    12481 taaggtatgc taaataggta ttccatttag tattagcatg ctctcccata attgattttt
    12541 gcgatgtgaa ataaatcaat ggaaagattg aaaatggtaa agctaaggaa agaaaaactt
    12601 gtgaatagac gattagttga tctaacgtat tttcttcgcc tccaactagt agggcgatag
    12661 caaaaattgg aagaagagtt aataatctag tacaaagcct tattaaccat tgaggtaatt
    12721 tgaagtgaag gaaaccttcc ataacgattt gtcctgttaa agtacctgta attgttgaat
    12781 tttgaccact tgctagtagg gcaactgcaa aaagggtaga aaggaaagaa ctggctactg
    12841 caccagtaat agttttatca gataaggcaa gatacatttg agaaaaggca ctgatatcat
    12901 tagcatgccc ataaaataga ctagctccta aaactaatag tagcgaattc acgacaaatg
    12961 caagtgatag ctgaatgtta gaatctaagg tcatgaaacg tacagctttt tttattgaag
    13021 atttattatg ataatccacg cgtctagtct gagataaaga agaatgtaaa taaagattat
    13081 gtggcatgac tgttgcgccg ataatgccaa gagcgagggt taaaggacta tttttccctt
    13141 catgtgatat gttaaaaagc tcatggtgtg gtaaaaatcc tttgaacata gcatctaaat
    13201 caggttgtga taaaacaact aaatatgtaa agattatcaa aattgtcaat atgagaacag
    13261 aaacaaaggc ttcaattttt tggacgccta acttcattaa gagtaaaagt aagaatacat
    13321 ccaaaatagt aatcatgata gacagtaaca ggggccagcc aaataataag tgaagagcaa
    13381 tggcagagcc aatgacttct gctaaatcag tagcgataag ggctaactca atgataataa
    13441 atagcaggta acgaagcggt tttgggagtc gagaagctgt tgcttgtgct aagtcctgtc
    13501 gtgtaacaat accaagcttg ccagccattt gttgtaattg catcgccatc aaagaagata
    13561 ctaaaacaac aaacaatagt aagtaacgat aggtagctcc accaataacg ctagtaatcc
    13621 agttacctgg atccatataa ccaactgcaa ccaaagctcc aggtccaaga aaagctctta
    13681 aagtgttcca aaaagagcta ttgtgaggaa cctcaacgct ctgattaact tcggataagg
    13741 atcttttgac ggaatgattt tgattcatgt aatactatac tttctagtaa tctcaagcca
    13801 ttataacaaa tagaaaata
//
LOCUS       DATROQ010000051        13523 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.052, whole genome shotgun sequence.
ACCESSION   DATROQ010000051 DATROQ010000000
VERSION     DATROQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13523
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.052"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            14..2086
                     /locus_tag="VBC94_001307"
     CDS             14..2086
                     /locus_tag="VBC94_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO6979578.1"
                     /translation="MKKKIILKSSVLGLVAGTSIMFSSVFADQVGVQVIGVNDFHGAL
                     DNTGTANMPDGKVTNAGTAAQLDAYMDDAQKDFKQTNPNGESIRVQAGDMVGASPANS
                     GLLQDEPTVKTFNAMNVEYGTLGNHEFDEGLAEYNRIVTGKAPAPDSNINNITKSYPH
                     EAAKQEIVVANVIDKVNKQIPYNWKPYTIKNIPVNNKSVNVGFIGIVTKDIPNLVLRK
                     NYEQYEFLDEAETIVKYAKELQAKNVKAIVVLAHVPATSKDDIAEGEAAEMMKKVNQL
                     FPENSVDIVFAGHNHQYTNGLVGKTRIVQALSQGKAYADVRGVLDTDTQDFIETPSAK
                     VIAVAPGKKTGSADIQAIVDQANTIVKQVTEAKIGTAEVSGMITRSVDQDNVSPVGSL
                     ITEAQLAIARKSWPDIDFAMTNNGGIRADLLIKPDGTITWGAAQAVQPFGNILQVVEI
                     TGRDLYKALNEQYDQKQNFFLQIAGLRYTYTDNKEGGEETPFKVVKAYKSNGEEINPD
                     AKYKLVINDFLFGGGDGFASFRNAKLLGAINPDTEVFMAYITDLEKAGKKVSIPNNKP
                     KIYVTMKMVNETITQNDGTYSIIKKLYLDRQGNIVAQEIVSDTLNQTKSKSTKINPVT
                     TIHKKQLHQFTAINPMRNYGKPSNSTTVKSKQLPKTNSEYGQSFLMSVFGVGLIGIAL
                     NTKKKHMK"
     gene            complement(2123..2533)
                     /locus_tag="VBC94_001308"
     CDS             complement(2123..2533)
                     /locus_tag="VBC94_001308"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO6979579.1"
                     /translation="MIKPIVRDTFFLQQKSQMASRADVSLAKDLQDTLHANQNYCVGM
                     AANMIGSLKRVIIINVGITNLVMFNPVLVAKSDPYETEESCLSLVGCRSTQRYHHITI
                     SYRDINWKEQQIKLTDFPAQICQHELDHLEGILI"
     gene            complement(2603..3952)
                     /gene="gdhA"
                     /locus_tag="VBC94_001309"
     CDS             complement(2603..3952)
                     /gene="gdhA"
                     /locus_tag="VBC94_001309"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEO6979580.1"
                     /translation="MTSGKTYVASVLEKVKKQNEHEEEFLQAVEEVFESLVPVFDKYP
                     QYIEENLLERLVEPERVISFRVPWVDDKGQVQVNRGYRVQFSSAIGPYKGGLRFHPTV
                     TQSIVKFLGFEQIFKNSLTGLPIGGGKGGSNFDPKGKSDNEVMRFTQSFMTELQKYIG
                     PDLDVPAGDIGVGGREIGYLYGQYKRLNGYQNGVLTGKGLTYGGSLARTEATGYGAVY
                     FAKEMLAARGQDLTGKVALVSGSGNVAIYATEKLQELGATVVAVSDSSGYVYDPDGID
                     LETLKQIKEVERARIVKYTEKHPKANFTPADQGSIWSIKADLAFPCATQNELDEEDAK
                     LLVENGVLAVTEGANMPSTLGAIKVFQKAGVAFGPAKAANAGGVAVSALEMAQNSSRR
                     AWTFEEVDQELQRIMKTIFVNASKAADEFGDSGNLVLGANIAGFLKVAQAMSAQGIV"
     gene            4120..4629
                     /locus_tag="VBC94_001310"
     CDS             4120..4629
                     /locus_tag="VBC94_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEO6979581.1"
                     /translation="MKWFPLVGGIIALLAGFYLFINPVTVVATLGWFIALFIFIYGIS
                     NLVRYLQADSYHRSPWYLVQSILAIIFGIILLSSSAMSLSNTVMTILAYWLLVSGILR
                     LLGGFQMQKINFPGAKSYKRSAYIALILGIILLVQPTLSAAIIGRFVAFILIAIGASA
                     IYTFTRINY"
     gene            complement(4670..6439)
                     /locus_tag="VBC94_001311"
     CDS             complement(4670..6439)
                     /locus_tag="VBC94_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979582.1"
                     /translation="MRKKSIFLRLWSYLTRYKATLFLAIFLKVLSSFMSILEPFILGL
                     AITELTANLVDMAKGVSGAELNVPYIAGILIIYFFRGVFYELGSYGSNYFMTTVVQKS
                     IRDIRHDLNRKINKVPVSYFDKHQFGDMLGRFTSDVETVSNALQQSFLQIINAFLSII
                     LVVVMVLYLNVPLAMIIIACIPVTYFSAQAILKRSQPYFKEQAKILGELNGFVQEKLT
                     GFNIIKLYGREEASSQEFRDITDNLRHVGFKASFISGIMMPVLNSISDFIYLIIAFVG
                     GLQVIAGTLTIGNMQAFVQYVWQISQPVQTITQLAGVLQSAKSSLERIFEVLDEEEEA
                     NQVTEKLSHDLTGQVSFHGVDFHYSPDKPLIRDFNLDVEPGQMIAIVGPTGAGKTTLI
                     NLLMRFYDVSEGAITVDGHDIRHLSRQDFRQQFGMVLQDAWLYEGTIKENLRFGNLEA
                     SDEDIVAAAKAANVDHFIRTLPGGYNMVMNQESSNISLGQKQLLTIARALLADPKILI
                     LDEATSSVDTRLELLIQKAMKKLMEGRTSFVIAHRLSTIQEADNILVLKDGQIIEQGN
                     HQKLLADKGFYYELYNSQFSNSK"
     gene            complement(6444..8183)
                     /locus_tag="VBC94_001312"
     CDS             complement(6444..8183)
                     /locus_tag="VBC94_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979583.1"
                     /translation="MAYIWSYLKRYPNWLWLDLLGAMLFVTVILGMPTALAGMIDNGV
                     TKGDRTGVYLWTFIMFIFVVLGIIGRITMTYASSRLTTTMIRDMRNDMYAKLQEYSHH
                     EYEQIGVSSLVTRMTSDTFVLMQFAEMSLRLGLVTPMVMIFSVVMILITSPSLAWLVA
                     VAMPLLVGVILYVAIKTKPLSERQQTMLDKINQYVRENLTGLRVVRAFARENFQSQKF
                     QVANQRYTDTSTGLFKLTGLTEPLFVQIIIAMIVAIVWFALDPLQRGAIKIGDLVAFI
                     EYSFHALFSFLLFANLFTMYPRMVVSSHRIREVMDMPISINPNTEGVTDTKLKGHLEF
                     DNVTFAYPGETESPVLHDISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYDVTLGKIL
                     VDGVDVRDYNLKSLRQKIGFIPQKALLFTGTIGENLKYGKADATIDDLRQAVDISQAK
                     EFIESHQEAFETHLAEGGSNLSGGQKQRLSIARAVVKDPDLYIFDDSFSALDYKTDAT
                     LRARLKEVTGDSTVLIVAQRVGTIMDADQIIVLDEGEIVGRGTHAQLIENNAIYREIA
                     ESQLKNQNLSEGE"
     gene            complement(8250..8723)
                     /locus_tag="VBC94_001313"
                     /pseudo
     CDS             complement(8250..8723)
                     /locus_tag="VBC94_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(8720..10588)
                     /locus_tag="VBC94_001314"
     CDS             complement(8720..10588)
                     /locus_tag="VBC94_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6979584.1"
                     /translation="MSDFLVDGLTKSVGDKTVFSNVSFIIHSLDRIGIIGVNGTGKTT
                     LLDVISGELGFDGDRSPFSSANDYKIAYLKQEPDFDDSQTILDTVLSSDLREMALIKE
                     YELLLNHYEESKQSRLEKVMAEMDSLDAWSIESEVKTVLSKLGITDLQLSVGELSGGL
                     RRRVQLAQVLLNDADLLLLDEPTNHLDIDTIAWLTNFLKNSKKTVLFITHDRYFLDNV
                     ATRIFELDKAQITEYQGNYQDYVRLRAEQDERDAASLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFQNLKNDLHQTSDTSDLEMTFETSRIGKKVINFENVSFSYPDKSILKD
                     FNLLIQNKDRIGIVGDNGVGKSTLLNLIVQDLQPDSGNVSIGETIRVGYFSQQLHNMD
                     GSKRVINYLQEVADEVKTSVGTTSVTELLEQFLFPRSTHGTQIAKLSGGEKKRLYLLK
                     ILIEKPNVLLLDEPTNDLDIATLTVLENFLQGFGGPVITVSHDRYFLDKVANKIIAFE
                     DNDIREFFGNYTDYLDEKAFNEQNNEVISKKESTKTSREKQSRKRMSYFEKQEWATIE
                     DDMMILENTITRIEDDMQTCGSDFTRLSDLQKELDAKNEALLEKYDRYEYLSGLDT"
     gene            complement(10599..11807)
                     /locus_tag="VBC94_001315"
     CDS             complement(10599..11807)
                     /locus_tag="VBC94_001315"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEO6979585.1"
                     /translation="MRLNYLPSEFQKALPILKKIKKAGYEAYFVGGSVRDVLLDRPIH
                     DVDIATSSYPEETKQIFKRTVDVGIEHGTVLVLEKGGEYEITTFRTEEVYVDYRRPSQ
                     VNFVRSLEEDLKRRDFTVNAFALNEDGEVIDLFHGLDDLDNHLLRAVGLASERFNEDA
                     LRIMRGLRFSASLNFDIETTTFEAMKKHAPLLEKISVERSFIEFDKLLLAPYWRKGML
                     ALIDSHAFNYLPCLKNRELQLSAFLSQLDKDFLFETSEQAWASLILSMEVEHTKTFLK
                     KWKTSTHFQKDVEHIVDVYRIREQMGLTKEHLYRYGKTIIKQAEGIRKARGLMVDFEK
                     IEQLDSELAIHDRHEIVVNGSTLIKKLGIKPGPQMGDIISQIELAIVLGQLINEEEAI
                     LHFVKQYLMD"
     gene            complement(11992..12840)
                     /locus_tag="VBC94_001316"
     CDS             complement(11992..12840)
                     /locus_tag="VBC94_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6979586.1"
                     /translation="MKLALITDTSAYLPEAIENHEDVYVLDIPIIIDGKTYIEGQNLT
                     LDQYYDKLAASKELPKTSQPSLAELDDLLCQLEKEGYTHVLGLFIAAGISGFWQNIQF
                     LIEEHPNLTIAFPDTKITSAPQGNLVRNALMCSREGMDFDVIVNKIQSQIEKIEGFIV
                     VNDLNHLVKGGRLSNGSAIIGNLLSIKPVLHFNEEGKIVVYEKVRTEKKALKRLAEIV
                     KEMTADGEYDIAIIHSRAQDKAEQLYNLLAKAGLKDDLEIVSFGGVIATHLGEGAVAF
                     GITPKN"
     gene            complement(12840..13220)
                     /locus_tag="VBC94_001317"
     CDS             complement(12840..13220)
                     /locus_tag="VBC94_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEO6979587.1"
                     /translation="MEVIREQEFVNQYHYDARNLEWEEENGTPKTNFEVTFQLANRDE
                     AAKVTSIVAVLQFVIVRDEFVISGVISQIAHIQGRLINEPSEFSQDEVENLAAPLLEI
                     VKRLTYEVTEIALDRPGVTLEFNS"
     gene            complement(13292..>13523)
                     /locus_tag="VBC94_001318"
     CDS             complement(13292..>13523)
                     /locus_tag="VBC94_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979588.1"
                     /translation="LDHLSAPLLKLFLKEKNVKGLSKMKRSDLDQAIAIAFTEEELGK
                     LFVVRGLALTEKGLAALSNNQEVIDRHPKKKF"
BASE COUNT         4355 a         2735 c         2094 g         4339 t
ORIGIN      
        1 acaggagatt taaatgaaaa agaaaattat tttgaaaagt agtgtccttg gtttagtcgc
       61 tgggacttct attatgttct caagcgtatt cgctgaccaa gtcggcgtcc aagttatagg
      121 cgtcaatgac tttcatggtg cacttgacaa tactggaaca gcaaatatgc ctgacggaaa
      181 agttactaat gctggcactg ctgctcaatt agatgcttat atggatgatg ctcaaaaaga
      241 tttcaaacaa actaacccta atggtgaaag cattagagtt caagctggtg atatggttgg
      301 agcaagtcca gctaactcag ggcttcttca agatgaacca accgttaaaa catttaatgc
      361 aatgaatgtt gagtatggca cattaggtaa ccatgaattt gatgaaggtt tggcagaata
      421 caatcgtatc gttactggaa aggcccctgc tccagattct aatataaata atattacgaa
      481 atcataccca cacgaagctg caaaacaaga aattgtagtg gcaaacgtta ttgataaagt
      541 taacaaacaa atcccttaca attggaaacc ttacactatt aaaaatattc ctgtaaataa
      601 caaaagtgtg aacgttggct ttatcggaat cgttaccaaa gacatcccaa accttgtctt
      661 acgtaaaaat tatgaacaat atgaattttt agatgaagct gaaacaatcg ttaaatacgc
      721 caaagaatta caagctaaaa atgtcaaggc tattgtagtc cttgctcatg tacctgcaac
      781 aagcaaggat gatattgctg aaggtgaagc agcagaaatg atgaaaaaag tcaatcaact
      841 cttccctgaa aatagcgtag atattgtctt tgctggacac aatcatcaat atacaaatgg
      901 tcttgttggt aaaactcgta ttgtacaagc gctctctcaa ggaaaagcct atgctgatgt
      961 acgtggtgtc ctagatactg atacacaaga tttcattgaa accccttcag ctaaagtaat
     1021 tgcagttgct cctggtaaaa aaacaggtag tgccgatatt caagccattg ttgaccaagc
     1081 taatactatc gttaaacaag taacagaagc taaaattggt actgccgagg taagtggcat
     1141 gattacgcgt tctgttgatc aagataatgt tagtccggta ggcagcctca tcacagaggc
     1201 tcaactagca attgctcgaa aaagctggcc agatatcgat tttgccatga caaataatgg
     1261 tggcattcgt gctgacttac tcatcaaacc agatggaaca atcacctggg gagctgcaca
     1321 agcagttcaa ccttttggta atatcttaca agtcgtcgaa attactggta gagatcttta
     1381 taaagcactc aacgaacaat acgaccaaaa acaaaatttc ttccttcaaa tagctggtct
     1441 gcgatacact tacacagata ataaagaggg cggggaagaa acaccattta aagttgtaaa
     1501 agcttataaa tcaaatggtg aggaaatcaa tcctgatgca aaatacaaat tagttatcaa
     1561 tgacttttta ttcggtggtg gtgatggctt tgcaagcttc agaaatgcca aacttctagg
     1621 agccattaat cccgatacag aggtatttat ggcctatatc actgatttag aaaaagctgg
     1681 taaaaaagtg agcattccaa ataataaacc taaaatctat gtcactatga agatggttaa
     1741 tgaaactatt acacaaaatg atggtacata tagcattatt aagaaacttt atttagatcg
     1801 acaaggaaat attgtagcac aagagattgt atcagacact ttaaaccaaa caaaatcaaa
     1861 atctacaaaa atcaaccctg taactacaat tcacaaaaaa caattacacc aatttacagc
     1921 tattaaccct atgagaaatt atggcaaacc atcaaactcc actactgtaa aatcaaaaca
     1981 attaccaaaa acaaactctg aatatggaca atcattcctt atgtctgtct ttggtgttgg
     2041 acttatagga attgctttaa atacaaagaa aaaacatatg aaataataaa aataggtttg
     2101 gataaaatat ccaaacctat ttttatatca atatgccttc caaatgatct aactcgtgtt
     2161 gacaaatttg agctggaaaa tctgtcaact tgatttgctg ttctttccaa ttgatatcac
     2221 gataagaaat cgtaatgtga tgatatcgtt gtgtacttct gcaacctacg agagaaaggc
     2281 aagattcttc tgtctcatat ggatccgatt ttgcaactaa tacagggtta aacattacta
     2341 aatttgtaat acccacattg atgataatga cacgcttcag actaccaatc atattggctg
     2401 ccatacctac acaataattt tgattagcat gcaaagtatc ctgtaaatct tttgctaagc
     2461 tcacatctgc tctactagcc atttgtgatt tttgttgtaa aaagaaagta tctctaacaa
     2521 ttggttttat catttatttt aacctaactc tattatgata atactagtat accataggtt
     2581 agctaaggac tatgataaga gattaaacaa taccttgtgc agacatagct tgagcaactt
     2641 ttaaaaagcc tgcaatgtta gcacctagaa ccaggttccc actatcgcca aattcatcag
     2701 ccgctttgga agcattcaca aaaatagttt tcataatacg ttgcaattct tgatcaactt
     2761 cttcaaaagt ccatgcccta cgactagaat tttgtgccat ttcaagcgct gaaacagcta
     2821 ctcctcctgc attggcagct ttagcaggac caaaagcaac ccctgccttt tggaaaactt
     2881 taatagcacc caaggtagat ggcatatttg ctccttctgt tactgccaag acaccatttt
     2941 caactaataa cttagcgtct tcttcgtcta attcgttttg agtagcacac gggaaagcta
     3001 aatctgcttt aatagaccag atactacctt gatctgctgg tgtgaaatta gcttttggat
     3061 gtttctccgt atatttaaca atacgtgctc tttcaacctc ttttatctgt ttcaatgttt
     3121 caaggtcaat accgtctgga tcataaacat aacctgaaga atctgacaca gctacaacag
     3181 ttgcacccaa ctcttgtaat ttttcagtag cataaatagc cacgttacca gatcccgaaa
     3241 ccagtgcaac tttgccagtt aaatcttgac cacgcgctgc cagcatttct ttagcaaaat
     3301 agactgcccc ataaccagtt gcttctgtac gagccaaaga gccaccataa gtcagtcctt
     3361 ttccggttaa aaccccattt tgataaccat ttaaacgctt atattgacca taaagataac
     3421 caatttcacg tccacctact ccaatatcgc cagctggcac atctaaatct ggtccaatgt
     3481 atttttgaag ttcggtcata aaactttgtg tgaaacgcat cacttcatta tctgatttcc
     3541 cttttggatc aaagttcgac cctcctttgc caccaccaat tggtagacct gtcagtgaat
     3601 tcttaaaaat ttgttcaaat cctaaaaatt tgacaataga ttgtgttact gtagggtgaa
     3661 aacgtaaacc acctttataa ggaccaatag ctgatgaaaa ttgtacacga tatcctcggt
     3721 taacttgaac ttggccttta tcatctaccc atggaactct aaaagaaatc acgcgctcag
     3781 gctcaaccaa acgttctaaa agattttctt caatgtactg agggtattta tcaaaaactg
     3841 gtactagtga ctcaaaaacc tcttcaaccg cttgtagaaa ttcttcctca tgctcatttt
     3901 gcttcttcac tttctctaaa acacttgcta catatgtctt tccgcttgtc atttattttc
     3961 ctccgtattg ttttaatata gttagcatta taaattgtca gaattttctt gtcaactatt
     4021 tgctatggta tcattattag gagcaatttt aaaggtaatt atttgacttt tactgacttt
     4081 cgttctataa tgagacaaat atttgattgg agtatctata tgaaatggtt cccacttgtt
     4141 ggaggtataa ttgccttatt agctggcttc tatctcttta taaatcctgt aacggttgtt
     4201 gcaactctgg gttggttcat tgctttattt atctttattt atggcatttc aaatttagta
     4261 cgctacttgc aagctgattc ttatcaccgt agcccttggt atctggttca gagtatcctt
     4321 gctattattt ttggtatcat tttactatcc agctcagcaa tgtcactttc aaatactgtc
     4381 atgactattt tagcatactg gctactggta tctggtattt tacgactttt gggtggtttc
     4441 caaatgcaaa aaattaattt cccaggtgcc aaatcctata aacgttcggc ttatattgca
     4501 ctcattttag gtattatttt actggttcaa cctaccttat cagcagccat cattggaaga
     4561 tttgtagcct ttatattaat agcaattgga gcttcggcta tttacacttt tactcgtatt
     4621 aattattaaa aggacatgtt tcgtgtcctt tttttatcta ttgatgacgt catttactat
     4681 ttgaaaattg gctattatat aattcgtaat aaaatccttt atctgctagg agtttctggt
     4741 gattaccttg ttcgatgatt tgaccatcct ttagaactaa aatgttgtct gcctcttgaa
     4801 tcgttgatag acggtgtgca atgacaaaac tagttcttcc ttccatcaat tttttcatag
     4861 ctttttgaat aagcaattcc aaccttgtat caacagatga agtcgcttca tctaaaatta
     4921 aaattttagg atctgctaat aaagcacgtg caatagtcaa cagttgcttt tgtcctaagg
     4981 agatattgct tgattcttga ttcatcacca tattgtagcc accaggtaac gttcgaataa
     5041 agtggtcaac attcgcagct ttggcagcag caactatatc ctcatcactg gcttcaagat
     5101 taccgaatct aagattttct tttattgtac cttcatataa ccaagcatct tgcaacacca
     5161 tgccaaattg ttgccggaaa tcttggcgtg ataggtgtct aatatcgtga ccatctacgg
     5221 tgatagcacc ttcactaaca tcgtagaatc tcatgagtaa gtttataagt gttgtcttac
     5281 ctgcacctgt tggccctaca attgcaatca tttgccctgg ttctacatct aagttaaagt
     5341 cgcggataag tggcttatcc ggagaatagt gaaaatcgac accatgaaag ctgacttgac
     5401 cggttaagtc atggctcaat ttttcagtga cttgattggc ttcctcttct tcatctaaga
     5461 cttcaaaaat acgttctaag gaagattttg cactttgcaa aacacctgct aattgtgtaa
     5521 tggtttgaac gggttgacta atttgccaaa catactgcac aaatgcctgc atgttaccaa
     5581 ttgttaaggt accagcaata acttgtaaac cgccaacaaa tgcaatgata agataaataa
     5641 aatcagagat actattcaat actggcatca taattcctga aatgaagcta gctttgaaac
     5701 ctacatgacg tagattatcc gttatatcac gaaattcttg actagacgcc tcttctctac
     5761 catataattt tatgatattg aaaccagtta atttctcttg tacaaaacca ttcagttctc
     5821 ccagaatctt tgcctgttct ttaaagtaag gttgggatct ttttagtatc gcttgtgcac
     5881 taaaataagt tacaggaata caagcaatga taatcatggc taatggtaca tttagataaa
     5941 gtaccataac gactactaga ataatactta aaaaagcatt gataatctgt aaaaagctct
     6001 gttgtaaagc atttgagacc gtttcaacat cacttgtaaa acgtcccaac atatctccaa
     6061 attggtgctt atcaaaatac gatacaggaa ctttattgat tttacgattc aaatcatgac
     6121 ggatatcacg gatacttttt tgaacgacag tagtcataaa ataatttgag ccataagaac
     6181 ctaattcata gaaaacacct ctgaaaaaat aaataatcag aataccagca atataaggaa
     6241 cgttcaattc tgccccagaa actcccttag ccatatcaac aaggttagca gtcaactctg
     6301 ttatcgctaa ccctaaaata aaaggctcca gaatactcat aaaactagat aaaactttca
     6361 aaaaaatcgc taagaaaaga gtagctttgt agcgagttag gtaagaccat aatctcaaaa
     6421 aaatagattt ttttctcata caatcactct ccttctgata agttttggtt cttcagttgt
     6481 gactcagcga tttcacgata aatagcatta ttttctatta attgagcgtg ggtaccacga
     6541 ccgacaattt cgccttcatc aaggacaata atctgatcag catccataat cgtacccacc
     6601 ctttgagcaa ctatcaaaac cgtagaatca ccggttactt cttttagacg cgctcttaaa
     6661 gtagcgtctg tcttataatc gagagcagaa aatgaatcat caaaaatata taaatctgga
     6721 tctttaacaa cagccctagc aatggataac cgttgttttt gacccccaga aagattgctc
     6781 ccaccttcag ctaaatgcgt ttcaaaggct tcttggtgac tctcaataaa ctctttagct
     6841 tgagaaatat caaccgcttg tctaagatca tcaatagtag catcagcttt tccatatttt
     6901 aaattctctc ctattgtccc tgtaaataaa agagcttttt gggggataaa tccaatcttt
     6961 tggcgaagtg atttaaggtt ataatctctt acatcaactc catctactaa gatttttcca
     7021 agtgtcacat cataaaaacg tggaatcaaa ttaacaagag aagattttcc tgaacctgtt
     7081 gaaccaataa aagcaattgt ttctccaggc ttagctttaa aagaaatatc atgcaaaacg
     7141 ggactctctg tttctcctgg ataagcgaat gttacattat caaattctaa atgcccttta
     7201 agtttcgtat ccgtaacacc ttcggtatta ggattaatag aaatcggcat atccattacc
     7261 tctctaatac gatggctaga taccaccata cgaggataca tagtaaaaag attggcaaat
     7321 agcaaaaatg aaaagagagc atggaagcta tattcgataa aagcaactaa atcccctatt
     7381 ttaatagcac ctctttgtaa gggatccaaa gcaaaccaaa cgatagccac aatcattgca
     7441 ataataattt gaacgaaaag tggttctgtt agccctgtta atttaaaaag accagttgaa
     7501 gtatctgtgt aacgttggtt agcgacttga aatttttgtg attgaaaatt ctctcttgca
     7561 aaggccctaa caacgcgtaa ccctgttaaa ttttcacgaa catattgatt gattttatca
     7621 agcatagtct gttgtctttc agataaaggt tttgttttta tagctacata taaaatgact
     7681 cctaccaaaa gaggcatcgc aaccgctaca agccaagcca aagatggact cgtaattagt
     7741 atcataacca cgctaaaaat cattaccata ggagttacta ggcctaaacg taaagacatt
     7801 tcagcaaatt gcatcaaaac aaaagtatcg cttgtcatac gtgtcactag tgaagataca
     7861 cctatctgtt catattcatg atgggagtat tcttgaagct tagcatacat atcattacgc
     7921 atatctctaa tcattgttgt cgttaagcga ctagatgcgt aagtcatcgt aatacgccca
     7981 ataataccta gtacaacaaa tataaacatg atgaacgtcc acagataaac tccagtccga
     8041 tcaccttttg taacgccatt atcaatcata cccgctaagg ctgtgggcat tcctaggata
     8101 accgtcacaa aaagcatagc tcctagtaaa tcaagccata accaatttgg gtaccttttc
     8161 aaataagacc aaatatatgc catatatgtc tccttttctt tcctcttatc tcaaaataat
     8221 taatccatgt tcactaatcc actttcaact tataaatctt taatcatcca aatatccata
     8281 gctgtatgac cttgatcatt tgctattggt tgcgataagg cacaatatcc catatgctta
     8341 taaaccgcag ttgccctact caaaatactg gctgtctcta aataaagttg tctataccca
     8401 atttttcgag cttctacttc aatcattttc actaaatcag tagcaagccc cttaccacgg
     8461 aaacgttctg caatgtacac cttctgcatc tctgcaatta gatttttcag cggaccaaag
     8521 ccgcttcagc caataatctc atctctctcc tcaatgacaa agaatcctga cttctctatt
     8581 ttttcgtagt atgaggtcaa gtgatctaag tgagggtctg aatatgctgt accaggttta
     8641 tctaaatcat aggcgcgtaa actctgtcga attaattgtg taactgcttg gtcatcattt
     8701 ttaataatcg gacggataat catgtgtcta acccactaag gtactcataa cggtcatact
     8761 tttctagaag tgcttcattt tttgcatcta attccttttg taaatcagat aaccttgtaa
     8821 aatcactacc acatgtttgc atatcatctt ctatacgagt gatagtattt tccaatatca
     8881 tcatatcgtc ttcaattgtc gcccattctt gtttttcaaa gtaagacatt cttttacgac
     8941 tttgcttttc acgacttgtc ttggtactct cttttttact gataacttca ttattttgct
     9001 cattaaatgc tttttcatct aaataatcag tataattacc aaaaaattca cggatatcgt
     9061 tatcttcaaa tgcaataatt ttattagcca ctttatctaa aaagtaacga tcgtgactaa
     9121 ctgtaatcac aggaccacca aagccttgta aaaaattttc aagaacagtt aatgtagcaa
     9181 tatctaagtc atttgtcggc tcatcaagta gtaacacgtt aggcttttca atcaggattt
     9241 ttaaaaggta aagtcgtttt ttctcaccac ctgataattt tgcaatttgt gttccatgtg
     9301 tcgaacgtgg aaagagaaat tgttccaata gttctgtcac acttgttgta ccgacactag
     9361 ttttaacctc atctgcaacc tcttgcaaat aattaataac acgttttgag ccatccatat
     9421 tatgaagttg ttgtgaaaag taacctacac gtatcgtttc accaatagag acattacccg
     9481 aatccggctg taaatcttga acaattaaat taagtaaggt tgactttcca acaccattat
     9541 ctccaacgat gccaatacgg tctttatttt gaattaacaa attaaagtct ttcaagatag
     9601 atttatctgg gtaagaaaaa gagacatttt caaaattaat aacctttttc ccaattcgac
     9661 ttgtttcaaa tgtcatttcc aaatcgcttg tatcgcttgt ttggtgtaaa tcgtttttta
     9721 gattttgaaa acgattaata cgagcctgtt gtttcgttgc acgagcttgt ggctgagtac
     9781 gcatccaagc tagttcctgt ttataaagct gtttcttttt atgtaaacta gcagcatcac
     9841 gctcgtcttg ttctgcacga agtcggacat aatcctgata attgccttga tattctgtaa
     9901 tctgtgcctt atctaattca aaaatacgtg ttgcaacatt gtctagaaaa taacgatcat
     9961 gagttataaa aagcactgtc tttttactat ttttcaaaaa attcgttaac catgcaatag
    10021 tgtcaatatc taagtggtta gtaggttcgt ctaagagcaa taaatctgca tcatttaata
    10081 atacttgcgc taattgaaca cgtcttcgta atcctcctga taattcacca accgacaact
    10141 gcaaatcagt aatacctaat ttggataata ctgttttgac ttcgctctca atagaccaag
    10201 catctaaaga atccatttct gccattactt tctctagacg tgattgctta ctttcttcgt
    10261 agtgattaag caataattca tattctttaa ttaaagccat ctctcttaag tcagaagaaa
    10321 gtacggtgtc caaaattgtc tgagaatcat caaagtctgg ttcttgtttt aaataagcaa
    10381 tcttataatc attagctgat gaaaaagggg aacgatcacc atcaaaacct aattcacccg
    10441 aaataacatc taatagtgtt gtctttccag ttccattgac accaataatc ccaatacggt
    10501 ctaaactatg gatgataaat gaaacattac taaagaccgt cttatcacca accgacttag
    10561 tcaatccatc tactaaaaaa tcgctcatat aatcctctct aatccatcaa gtactgctta
    10621 acaaaatgta aaatagcctc ttcttcatta atcagttgtc ctaaaacaat ggctaattca
    10681 atttgagaga taatatctcc catctgtgga ccaggtttta ttcctaattt cttgattaag
    10741 gtgctaccat tgacaactat ctcatgccta tcatggatag ctaactcact atctagttgt
    10801 tctatttttt cgaaatcaac catcaagcct cttgctttgc gaataccttc cgcttgtttt
    10861 attatagttt ttccataacg ataaagatgt tctttagtca atcccatttg ttcacgaata
    10921 cgataaacat ccactatatg ctcaacatct ttttgaaagt gagttgatgt cttccatttt
    10981 tttaaaaaag tctttgtgtg ttcaacttcc atactcaaga tgagtgatgc ccaagcttgt
    11041 tctgatgtct caaataaaaa atctttatct aactgggaca aaaaggcgct taattgtaat
    11101 tccctatttt ttaagcatgg tagataatta aaagcatgac tatctattag tgccagcatt
    11161 cctttacgcc aataaggtgc taatagaagt ttatcaaatt caataaaaga acgctcaaca
    11221 gatattttct ccaataatgg cgcgtgcttt ttcattgcct caaaagttgt tgtttcgata
    11281 tcaaaattta gactagcaga aaaacgcaaa cctcgcataa ttcgaagagc atcttcatta
    11341 aaacgctcac tggcgagtcc tactgctcta agaaggtgat tatctaaatc atccaatcca
    11401 tgaaagagat caataacttc tccatcttca tttaaagcaa aggcattaac tgtgaaatct
    11461 cgacgtttta gatcttcttc tagcgaccta acaaaattga cctgactcgg acgcctataa
    11521 tcaacataaa cctcctcagt cctaaaagtg gtaatctcat attcaccacc tttttccaat
    11581 actaagactg ttccatgttc aataccaaca tcaactgtcc ttttaaaaat ctgctttgtt
    11641 tcttctggat aagatgacgt tgctatatca acatcatgaa ttggacggtc aagtaaaaca
    11701 tcccgaacac taccacctac aaagtatgct tcataaccgg ctttttttat cttttttaat
    11761 attggtaaag ccttctgaaa ctcagaaggc aaataattta atctcatcat actattccaa
    11821 atctaaacga actaaagtcg cattattatt tcttataaat ggattcagaa acaaaaagaa
    11881 ctgcctaagg gacactccct acctactcaa attatcaaaa aagaatctcc taacttcttc
    11941 tttgatatct gcaataagga tcaacctcca agcaactctt tttatcacac atcaattttt
    12001 tggggtaata ccaaaggcaa ccgcaccttc accaagatga gttgcaataa cgccaccaaa
    12061 tgatacaatt tctaaatcgt ctttaagacc agctttagct aacaaattat ataattgttc
    12121 tgccttatct tgtgctctag agtgaataat agcaatatca tattcaccat ctgctgtcat
    12181 ttctttgaca atctcagcca agcgttttaa ggcttttttc tctgtacgca ctttctcata
    12241 gacaacgatt ttaccttctt cattaaaatg taagaccggc ttgatactta aaaggttacc
    12301 aatgatagct gaaccgtttg acaatctacc accttttact agatgattca aatcattaac
    12361 aacaataaat ccttcaattt tttcaatttg tgattgtatc ttgttaacta tgacatcaaa
    12421 atccatccct tcacgtgaac acatcaaagc atttctaact aaattgccct gtggtgctga
    12481 agttatttta gtatccggaa aagcaattgt caaatttgga tgttcttcaa taagaaactg
    12541 aatattctgc cagaaacctg atatccccgc tgcaatgaat aaaccaagga cgtgtgtata
    12601 cccttctttc tccaattgac ataagagatc atctagttca gctaagctag gctgacttgt
    12661 tttaggtaat tcttttgatg ccgctagctt atcataatat tggtctaatg tcagattttg
    12721 tccctcaata taagttttac catcaatgat aattggaata tctaaaacat agacatcttc
    12781 atgattttcg atagcctcag gcaagtaagc tgaggtatcc gtaataagtg ctaatttcat
    12841 taactattaa actccaatgt tactcctggt ctatctaaag caatttctgt aacttcataa
    12901 gtcagacgtt taacgatttc taaaagagga gctgctagat tctcaacttc atcttgacta
    12961 aactcactag gctcattgat aaggcgccct tggatatgag ctatttgtga aatgacacca
    13021 gaaataacaa actcatcacg tacaataaca aattgtaaca ctgctacaat gctcgttact
    13081 tttgctgctt catctctatt agctaattgg aaagtaactt caaaatttgt ttttggggta
    13141 ccattttcct cttcccattc taaattgcgc gcatcatagt ggtattgatt gacaaattct
    13201 tgttcacgaa tgacttccat gtattttctc cttgatagac aatatgtatt attatagcat
    13261 aaatttgtag attcttaaag caggtgggaa tctagaactt cttcttagga tgtctatcaa
    13321 tcacttcttg attgttactc aaggctgcta aacctttttc tgtaagtgcc aaccctctga
    13381 caacaaacaa cttccctaat tcctcttctg taaaagcaat agctattgcc tgatctaaat
    13441 cagagcgttt catctttgac aagcctttta cgtttttttc ttttaagaat agttttaaaa
    13501 gtggagcaga taagtgatct aaa
//
LOCUS       DATROQ010000052        13476 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.053, whole genome shotgun sequence.
ACCESSION   DATROQ010000052 DATROQ010000000
VERSION     DATROQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13476
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.053"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            92..793
                     /locus_tag="VBC94_001319"
     CDS             92..793
                     /locus_tag="VBC94_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEO6979589.1"
                     /translation="MRVITVKNDIEGGKIAFTLLEEKMKTGAQTLGLATGSSPITFYE
                     EIVKSNLDFSNMVSINLDEYVGIAASNDQSYSYFMHKHLFDAKPFKENNLPNGLAKDL
                     KKEIKRYDAVINANPIDFQILGIGRNGHIGFNEPGTPFDITTHVVDLAPSTIEANSRF
                     FNSIDDVPKQALSMGIGSIMKSKTIILVAYGIEKAEAIASMIKGPITEDMPASILQKH
                     DDVVIIVDEAAASKL"
     gene            complement(865..1821)
                     /locus_tag="VBC94_001320"
     CDS             complement(865..1821)
                     /locus_tag="VBC94_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO6979590.1"
                     /translation="MGLLVDGKWVDQWYDTASTGGKFVRTVTQFRHWVTKDGSAGPSG
                     DAGFKAESGRYHLYVSLACPWASRVLIMRKLKNLESHISISIVNPLMLENGWTFQEYK
                     GVIPDMINQSQYLYQIYQASQSDYTGRVTVPVLWDKKCHTIVNNESSEIMRMLNTAFN
                     HITGNTDDYYPDSLQGQIDEMNNFIYPKINNGVYKAGFATSQNVYQKEVETLFTALDQ
                     LEKHLSDNHYLVGEQFTEADIRLFTTLVRFDTVYYGHFKCNLKALHDYPHLWHYTKRI
                     YNLPGIAETINFDHIKKHYYGSHKTINPTGIIPAGPNLDWTI"
     gene            1917..2636
                     /locus_tag="VBC94_001321"
     CDS             1917..2636
                     /locus_tag="VBC94_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6979591.1"
                     /translation="MRLDKLLGQAGFGSRNQVKKLIRSRQVSVDGQIVTKDNVIVDSG
                     LQSIFVGKERVCLKESSYYLLYKPSGVVSAVRDSEHKTVIDLISEKDKVEGLYPIGRL
                     DRDTEGLLIITNNGPLGYRMLHPKHHVAKTYYVEVNGFLERDAITFFEEGVVFDDGTK
                     CKPAELTIDTANNDKSTARITITEGKFHQVKKMFLAYGVKVIYLRRISFGDLRLDMNL
                     KPGQYRRLRDSEAAILKRYLD"
     gene            2709..3860
                     /locus_tag="VBC94_001322"
     CDS             2709..3860
                     /locus_tag="VBC94_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6979592.1"
                     /translation="MNSKLSLKLTILSISIFLMSHLAIAPAIPKLYAFYHTQNPHIGL
                     ASVESLVTIPAMMITIFVILSNFVVTKLGKKNTVLLGLCLILMSGFISFFTSNFSLAM
                     ASRLLLGIGIGLYNSLSISIITDLYEADERASMIGLRTASLNIGKALTTFIVGLVLAI
                     GVNYIYLVYLLVIPVFFFFWKNVPEVENQTHTLKASTTFDTKAALLMLITFLVGIAYI
                     GATVKIPTLLVTKYHYATSFSSNMLTLLAFSGILVGSVFGKLVKAFQEKTLLIMILAM
                     GIGNVLFALANNQIIFIVASILIGASFVGTMSSVFFYISKNYAKEHNNFITSLALTAG
                     NIGVILTPLILTKLPSQLHLEPFITPFLITSGLMVINVFVYLVLMSKNK"
     gene            3912..4859
                     /locus_tag="VBC94_001323"
     CDS             3912..4859
                     /locus_tag="VBC94_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEO6979593.1"
                     /translation="MLIAKDKQGNLINLLESHPGKGQYFCPTCCSAVRLKAGRIMRRH
                     FAHISLKNCQFHHENESNEHLQLKAKLYMSLSRENETMLEHHLPEINQIADLFVNETL
                     ALEVQCSRLSEQRLRERTKAYLQADFQVRWLLGEKLWLKHRLTNLHKQFLQFSQSIGF
                     HIWELDLRLEVLRLKYLIYEDLRGHVYYLSKTCPLSGDVLAFLKWPYQSKNLNFYKVK
                     QDRNIRDYVRQQLRYGNQFWLRKQEKAYLSGQNLLTQELMMFFPQIQPPRVDTDFCQI
                     TNSLTSFYQNFTNYYQKNKNNLDQTLYPPVFYDKIVTKN"
     gene            4875..6680
                     /gene="pepF"
                     /locus_tag="VBC94_001324"
     CDS             4875..6680
                     /gene="pepF"
                     /locus_tag="VBC94_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO6979594.1"
                     /translation="MSDNRSHIEEKYQWDLTTVFATDELWETEVVELTQAIDDAKGFS
                     GHLLDSSQSLLEITEVELELSRRLEKVYVYASMKNDQDTTVAKYQEFQAKATALYAKF
                     SETFSFYEPELLQLSESDYQSFLLEMPDLQKYDHFFEKIFANKPHVLSQNEEELLAGA
                     SEIFGAAGETFEILDNADMVFPVVKNAKGEEVELTHGNFISLMESSDRTVRKEAYQAM
                     YSTYEQFQHTYAKTLQTNVKSQNFKARVHHYQSARQSALSANFIPEEVYETLIKTVNH
                     HLPLLHRYMKLRQKVLGLDDLKMYDVYTPLSQMDMSFTYDEALKKSEKVLAIFGEAYS
                     ERVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRENQPYVYGDYSIFLAEIASTTNENILTETLLKEVKDDKNRFAILNHYLDGFK
                     GTIFRQTQFAEFEHAIHVADQEGQVLTSEYLNNLYAELNEKYYGLTKEDNHFIQYEWA
                     RIPHFYYNYYVFQYATGFAAANYLAERIVNGNPEDKEAYLNYLKAGNSDYPLNVIAKA
                     GVDMTSADYLDAAFRVFEERLVELENLVAKGVHND"
     gene            6875..7501
                     /locus_tag="VBC94_001325"
     CDS             6875..7501
                     /locus_tag="VBC94_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEO6979595.1"
                     /translation="MKKLTFIWDLDGTLIDSYVPIMEALEETYRHFGLIFDKELIHEY
                     ILQESVGQLLVNLSEEEQIPHEKLKAYFTKEQESRDSKIHLMPYAKEILEWTKEQDIP
                     NFMYTHKGASTHSVLETLQISHYFDEILTGVSGFERKPHPQGINYLVKRYSLDKSMTY
                     YIGDRPLDLEVAQNAGIKSINLRLENSKENYNISSLKDIISLDFTRLD"
     gene            7575..8282
                     /locus_tag="VBC94_001326"
     CDS             7575..8282
                     /locus_tag="VBC94_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEO6979596.1"
                     /translation="MVQSYSKNANHNMRRPVVKEEIVQYMRQHQKQNNGCLAELEAFA
                     KQENIPIIPHETATYFRFLMQTLQPKHILEIGTAIGFSALLMAENAPEAKITTIDRNE
                     EMIALAKENFAKYDNHNQITLLEGDAVDVLQTLDKSYDFVFMDSAKSKYIVFLPQVLK
                     HLDVGGVVVLDDIFQGGDIAKPIDEVRRGQRTIYRGLQRLFDSTLQHPDLTATLVPLG
                     DGLLMIRKNADHIVLED"
     gene            8343..9272
                     /gene="prsA"
                     /locus_tag="VBC94_001327"
     CDS             8343..9272
                     /gene="prsA"
                     /locus_tag="VBC94_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEO6979597.1"
                     /translation="MKTRSKLAAGFLTLMSVATLAACSGKTSNGTNVVTMKGDTITVS
                     DFYDQVKTSKAAQQSMLTLILSRVFDTQYGDKVSDKKVSEAYNKTAKGYGNSFSSALS
                     QAGLTPEGYKQQIRTTMLVEYAVKEAAKKELTEANYKEAYKNYTPETSVQVIKLDAED
                     KAKSVLKDVKADGADFAKIAKEKTTATDKKVEYKFDSAGTSLPKEVMSAAFKLDKNGV
                     SDVVSTVDSTTYKTSYYIIKVTDKTEKKSDWKSYKNRLKEVILKDKTSDRSFQNKVIS
                     KALEKANVKIKDKAFAGILSQYATTSGSSSLKK"
     gene            9514..9999
                     /locus_tag="VBC94_001328"
     CDS             9514..9999
                     /locus_tag="VBC94_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEO6979598.1"
                     /translation="MATFQIKEKMFSLGGKFTITDLTGLPCYHVEGSLFPLPKTFKVF
                     DEEEHLISQIEKKVLSFLPKFNVTLANGNHFTIKKDFSFLKPHYTIEDLDMEVKGNFW
                     DMDFQLLKDNQVIANISQQWFRMTSTYQVEVYSETYNDLTISLVIAIDYVKELEKNAS
                     N"
     gene            10015..12633
                     /gene="alaS"
                     /locus_tag="VBC94_001329"
     CDS             10015..12633
                     /gene="alaS"
                     /locus_tag="VBC94_001329"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEO6979599.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHSVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPEWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDATAKVQALS
                     DSLRDLQKENVELKEKAAAAAAGDVFKDIQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKGLAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAENL"
     gene            12715..13431
                     /locus_tag="VBC94_001330"
     CDS             12715..13431
                     /locus_tag="VBC94_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEO6979600.1"
                     /translation="MVGLGTVINVILIIVGGFVGLFLKNFLKESLQKSLMQAMGVAVL
                     FISISGVLEKMMLVEKSHLISNHTNMMIITLALGTVLGELLSLDSYIDRFGDYLKQKT
                     GSGNDIKFVEAFVTSTCTVCIGAMAVVGSIQDGIAADHSILFAKGMLDMIIIAIMTVS
                     LGKGALFSALPVALLQGSLTIVAFFMGSLLNPSSLDYLNLVGNMLIFCVGVNLLFNLN
                     IKVINMLPAIILAILWGSFI"
BASE COUNT         4495 a         2168 c         2627 g         4186 t
ORIGIN      
        1 taacgaaatt aagaagtttt tattgacaaa aattggtata taccatataa tataatcata
       61 aaggtctata ccaaataaaa ggagagtaaa catgagagtt attacagtta agaacgacat
      121 tgagggggga aaaattgcat ttactctttt agaagagaaa atgaaaactg gtgctcaaac
      181 tttaggattg gcaacaggaa gtagcccaat caccttttac gaagagattg taaaaagtaa
      241 ccttgatttt tcaaatatgg tttctattaa ccttgatgaa tatgttggaa ttgccgcaag
      301 taatgatcaa agttactctt attttatgca taaacatctt tttgatgcta aaccttttaa
      361 ggaaaataac cttccaaacg gcttggctaa agatttgaaa aaagaaataa aacgatacga
      421 tgctgtcata aatgctaatc caattgattt tcaaattctt ggaattggtc gtaacggtca
      481 tattggtttt aatgaaccag gtacaccttt tgatattaca acacatgtgg ttgacttagc
      541 tccaagtaca attgaagcta atagtcgttt ctttaatagt attgatgatg taccaaagca
      601 agccttatca atgggtatag gttctattat gaaatcaaaa acaatcatat tagtggcgta
      661 tggtattgaa aaagcagaag cgattgcaag tatgattaaa ggacctatca cagaagatat
      721 gccagcaagt atcctccaaa aacatgatga tgttgtgatt attgtggatg aagcagcggc
      781 atcaaagttg taaagtaaat aataaaaaag taaggatgaa tctcaatgag aaaaaattca
      841 ttcttacttt tttattatgc tttattaaat tgtccagtct aagttaggtc cagctggaat
      901 aataccggta gggttaatgg ttttatggct accgtaatag tgttttttga tatgatcaaa
      961 attaattgtc tcagcaattc caggtaggtt gtaaatacgt tttgtataat gccataaatg
     1021 tgggtaatca tgtagcgctt ttaaattaca tttaaaatga ccataataaa cggtgtcaaa
     1081 gcgaactaaa gttgtgaaaa ggcgaatatc agcttcggta aattgttctc caacaagata
     1141 atggttatca gataagtgtt tttctaactg atctaaagct gtgaataagg tttcaacttc
     1201 cttttgataa acgttctgag aagtagcaaa accagcttta tagacaccat tatttatttt
     1261 tggataaata aaattattca tttcgtcaat ttgtccttgt aaggaatcgg gatagtaatc
     1321 gtcagtattt ccagtaatat gattaaatgc agtatttaac atgcgcataa tttcagatga
     1381 ttcattgtta acgattgtat ggcatttttt atcccaaaga acagggactg tcacgcgtcc
     1441 tgtataatca ctttgacttg cttgatagat ttggtaaagg tattggcttt ggttaatcat
     1501 atcaggaata acgcctttat attcttgaaa agtccaacca ttctccaaca ttaaagggtt
     1561 aacaatggag atagagatat gagattctag atttttaagc tttctcatga ttaatacacg
     1621 tgatgcccaa gggcaggcta gtgaaacgta gagatgatat cgtccagatt ctgctttaaa
     1681 acctgcatcc ccactaggcc ctgctgaacc atctttagta acccaatgac ggaattgtgt
     1741 aacggttcgg acgaatttac caccagttga tgcagtatca taccattgat caacccattt
     1801 gccatctact aaaagtccca tgataatctc ctttgttttt ctattactaa ttagtagtat
     1861 aaagatttta aaaggaatta gcaaatgttt aacttacagt atggtaaact aagggtatgc
     1921 gtttagataa attattaggt caggcaggat ttgggtctag aaatcaggtt aagaaattaa
     1981 ttcgttctag gcaagtttct gtagatggtc aaattgttac aaaggataat gttattgtag
     2041 atagtggtct acaatcgatt tttgtaggaa aagagagagt ctgcctaaaa gaaagttctt
     2101 attaccttct ttataagcca tcaggagttg tttcagcagt tagagattca gaacataaaa
     2161 cagtcataga cttaattagc gaaaaagata aggtggaggg attgtatccg attggtcgtc
     2221 tagatcgtga tacggaaggg cttttaataa tcacgaataa tggtccactg ggttaccgaa
     2281 tgttacatcc aaaacaccac gttgcaaaaa cgtattatgt tgaagtaaat ggtttcttgg
     2341 aaagagatgc tattaccttt tttgaagaag gagttgtatt tgacgatgga acaaagtgta
     2401 aaccagctga attaacgatt gatacggcta ataatgacaa aagtaccgca agaataacta
     2461 tcacagaagg gaaatttcac caagtaaaaa agatgtttct tgcatatggc gttaaagtta
     2521 tatacttgag gcgtatttct tttggtgact tgaggcttga tatgaatttg aaaccaggtc
     2581 agtatcgtag attgagagat tcagaggcag ctatcttaaa aaggtattta gattagtgaa
     2641 acgactcact tgaccttgct tagtattctt catatactta taagtgatta ttttaaagga
     2701 gaaaactatt gaattcaaaa ctttcattga aattgactat tttatcgatt tctatctttt
     2761 tgatgtctca cttagcaatt gctcctgcaa ttcctaaatt gtatgccttt taccatactc
     2821 aaaaccctca tattggacta gcttcagtag agagtttagt tactattcca gctatgatga
     2881 ttactatttt tgttattttg agtaattttg tagtcacaaa acttggcaaa aaaaacacgg
     2941 tcttacttgg tttatgttta atcttaatgt ctgggtttat ctcatttttt acaagtaatt
     3001 tttcacttgc gatggctagt cgactacttt tgggtattgg tattggtctc tataattcgc
     3061 tatcaattag tatcataacg gatttatatg aggctgatga gagagccagt atgattggtc
     3121 ttagaacagc atcacttaat atcggtaaag ccttaactac atttattgtt ggattggttt
     3181 tagctattgg tgtgaattat atttatttgg tttaccttct ggttattcct gttttctttt
     3241 tcttttggaa aaatgttcca gaggttgaaa atcaaacaca tacccttaaa gctagtacaa
     3301 catttgatac taaagcggct ttgttgatgc tcatcacgtt cttagtaggt attgcttata
     3361 taggagcaac tgtcaaaatt ccaaccttac tagtgacaaa atatcactac gcgacgagct
     3421 tctctagtaa tatgttaact ctccttgctt ttagtggtat tttagttgga tctgtatttg
     3481 gaaaattagt caaagcattc caagagaaga cgctattaat catgattcta gctatgggaa
     3541 taggtaatgt gctatttgct ttagctaata atcagatcat ttttatcgta gctagtattt
     3601 taataggagc tagttttgtt ggcaccatgt cttctgtatt cttttatatt tcaaaaaact
     3661 atgcaaaaga acataataac tttatcacaa gccttgcctt aacagctggt aatataggag
     3721 ttattttgac accgcttatt ttaacgaaac ttccaagcca gctacatctg gaacctttta
     3781 taacaccatt tttaataaca agtgggctga tggtgattaa tgtctttgtt tacctagttt
     3841 taatgtcaaa aaataaataa aaagttatcc gaaaggatag cttttttcga atagtaaaag
     3901 taggagaaaa aatgttaatt gctaaagaca aacaaggaaa tttaattaat ctactcgagt
     3961 ctcatccggg aaagggacag tatttttgtc caacttgttg ttcagctgta aggttaaaag
     4021 caggtcgaat catgagaaga cactttgcac atattagtct aaaaaattgt cagtttcatc
     4081 atgaaaatga gtctaatgag cacttgcaat taaaagcaaa gttatatatg agtttaagta
     4141 gagagaatga aactatgctc gagcatcatt tgccagaaat taatcagatt gcagatttat
     4201 ttgtgaatga gacattggct ttagaagttc agtgtagccg actatcagag cagcgactca
     4261 gggagcgaac aaaagcatat ctacaggcag actttcaagt gagatggtta ctaggggaaa
     4321 aactctggtt aaagcatcgt ttaacaaatc tacataaaca atttttacaa ttttctcaaa
     4381 gtatcggatt tcatatttgg gagttagatt tgaggttaga agtactgaga ctaaaatatt
     4441 tgatttacga agatttacgc ggacatgtct attacttaag taaaacttgc cctcttagtg
     4501 gagacgttct agcatttttg aagtggccat accaatcaaa aaatctaaat ttttataagg
     4561 ttaaacaaga cagaaatatc agagattatg tgcgtcagca gttacgctat ggcaatcaat
     4621 tttggttacg aaaacaagaa aaggcctacc tttctggtca gaatctatta acccaagagc
     4681 taatgatgtt ttttcctcaa atacagccac cacgtgtaga tactgatttt tgtcaaataa
     4741 caaattctct gacatcgttt tatcagaatt ttacaaatta ttatcaaaaa aataagaata
     4801 acttggacca aacactttat ccccctgtat tttatgataa aatagtcact aagaattaaa
     4861 ctggaggata caaaatgtca gacaatcgct cacatattga agaaaaatac caatgggatt
     4921 taactactgt ctttgcgact gatgagttat gggaaactga agtggtagag cttactcaag
     4981 ccatcgatga cgcaaagggt ttttcaggtc atctgttgga ctcaagccaa tctttattgg
     5041 agattacgga ggttgaacta gagttatctc gccgtttaga aaaagtttat gtctatgctt
     5101 caatgaaaaa tgaccaagac accactgttg cgaaatatca agaatttcaa gcgaaagcga
     5161 cagcgctcta tgcaaaattt agtgaaacat tttcgtttta cgagccagaa ctattgcaac
     5221 tttcagagtc tgattaccag tcattccttt tagaaatgcc tgacttgcaa aaatatgatc
     5281 atttctttga gaaaatattt gcaaataaac ctcatgtctt atctcaaaac gaagaagaat
     5341 tgttagctgg tgctagtgag atttttggag cagctggtga gacatttgaa atccttgata
     5401 atgcagatat ggtcttccca gttgtaaaaa atgcaaaagg tgaggaagtt gagcttacac
     5461 atggtaattt catttccctt atggaatcta gcgataggac ggttcgaaaa gaagcatatc
     5521 aagctatgta tagcacatat gaacaattcc agcatactta cgctaaaaca ttacaaacaa
     5581 atgtgaaatc tcaaaatttt aaggctcgtg tgcatcatta tcaatcagca cgccaatcag
     5641 ctctatctgc caattttatt ccagaagagg tctacgaaac tctaattaag acagttaatc
     5701 atcatttacc gctcttacat cggtatatga agttgcgtca aaaagttctt gggctagatg
     5761 atttgaagat gtatgacgta tatacacccc tttctcaaat ggatatgagt tttacttatg
     5821 atgaagctct aaaaaaatca gagaaggttc tggctatttt tggcgaagca tattctgaac
     5881 gtgttcatcg tgcttttact gaacgttgga ttgatgtcca tgttaacaaa ggaaagaggt
     5941 caggcgctta ttcaggtgga tcttatgaca ctaatgcttt catgcttctt aactggcaag
     6001 atactttaga caatctttat acgctggtgc atgaaactgg acatagtcta cactcgacgt
     6061 ttactcgtga aaatcaacct tatgtttatg gtgattattc tattttccta gctgaaattg
     6121 catcaacaac aaatgaaaat atattaacag aaaccctttt gaaagaagtt aaagatgata
     6181 aaaatcgctt tgcaattctc aatcattatt tggacggttt caaaggaaca atttttagac
     6241 aaacacagtt cgctgaattc gaacatgcca ttcatgtagc tgatcaggag gggcaagtac
     6301 taactagcga atacttaaac aacctttacg ctgagcttaa tgaaaaatat tatggtttga
     6361 ctaaagaaga taatcatttt attcaatatg aatgggcaag aattccacac ttttactaca
     6421 attattatgt tttccaatat gcaaccggct tcgcagcagc aaattattta gcagagcgta
     6481 ttgtaaacgg taatccggag gataaagagg cctacctaaa ttaccttaaa gctgggaatt
     6541 cagattaccc tctaaacgtc attgctaaag ccggtgttga tatgactagt gcagactatc
     6601 tggatgcagc ttttagagta tttgaagagc gtttagtgga actggaaaat cttgtagcaa
     6661 aaggcgtcca taatgattaa gttgaaaata tagctattga agtaaaacca gtcaactaga
     6721 ctggtttttg atagtgatca gttattttat agtttgtttt gagagtggtt ttggtaaagt
     6781 tagttagtgg aaaactcaat aggtggaaat acctacccaa gctatgattt ttatgagacc
     6841 aaaactgcta ttgtcaaaaa agaagggaaa taggatgaaa aagcttactt ttatttggga
     6901 tttagatggg acattaatag attcgtatgt accaattatg gaagctcttg aagaaaccta
     6961 tcgtcatttt ggcttaatat ttgataaaga attaatccat gaatatattt tacaggaatc
     7021 agtggggcaa ttattggtaa acctttcaga ggaagagcaa atacctcatg aaaaactgaa
     7081 agcatatttt acaaaagaac aagaaagtcg agattctaaa atacatttaa tgccatatgc
     7141 aaaagagatt ttagaatgga ccaaagaaca agatattccc aattttatgt atacacataa
     7201 aggagcaagt acgcattcag tgttggaaac cttgcagatc tctcattatt ttgatgaaat
     7261 tttaactggt gtttcgggat tcgagcgaaa accacatcca caagggatta attatttagt
     7321 taaacgatat tctttagata aatcaatgac ttattacata ggagatcgtc cactagattt
     7381 ggaggttgct caaaatgctg gtataaaatc cataaactta aggttagaga attccaaaga
     7441 aaactataat atttcaagtc tcaaagatat aatatcactt gatttcactc gtttggatta
     7501 aatcattctc agagttagac attttttgct ataataaaaa agttattatt taaaatagaa
     7561 aagaggagcc aaaagtggtt caatcttaca gtaaaaatgc taatcataat atgcgccgtc
     7621 cagtagttaa agaagaaatt gttcaatata tgcgtcagca ccaaaaacaa aataatggct
     7681 gtttagcaga gttagaagca tttgctaaac aggaaaatat ccccattata cctcatgaaa
     7741 cagcgactta ttttcgtttt ttgatgcaaa cattacaacc taagcacatt ttagaaatcg
     7801 gaacagctat tggtttttcg gcccttttga tggcagaaaa cgctcctgaa gcaaagataa
     7861 caacaatcga tcgtaatgaa gagatgattg ccttggctaa ggaaaacttt gcaaaatatg
     7921 ataatcacaa tcagataacc cttttggaag gcgatgctgt ggatgtgtta caaacattgg
     7981 ataaatctta cgattttgta tttatggatt ctgctaagtc taaatatatc gtttttttac
     8041 ctcaagtatt aaagcattta gatgttggag gagtagttgt tttagatgat attttccaag
     8101 gtggagatat tgcaaaacct atcgatgaag tgagaagggg tcagcgaact atctaccgag
     8161 gtctgcaacg tttatttgac tcgactttgc aacatcctga tttaactgct actttggtac
     8221 ctttgggaga tggtttattg atgattcgta aaaatgcaga tcatattgta ctagaagatt
     8281 aaggcatttt taagaaatta tgatataata atggagttaa tgacaaaaag gagttataaa
     8341 tcatgaaaac aagatcaaaa cttgcagcag gctttttgac acttatgtca gttgccacat
     8401 tagctgcctg ctcaggtaaa acatcaaatg gaacaaatgt tgttacaatg aagggcgaca
     8461 ctattacagt ctctgatttt tatgatcaag taaaaacatc aaaagctgca caacaatcaa
     8521 tgcttacatt gatcctctca cgtgtttttg atacacagta tggtgataaa gtttcagata
     8581 aaaaagtatc agaagcttat aataagacag ctaaaggcta tggtaattca ttttcaagcg
     8641 cactttcaca agcaggtttg actccggaag gttacaaaca acaaattcgc acaactatgc
     8701 ttgtggaata tgctgtaaaa gaagcagcta agaaagaatt aacagaagca aactataaag
     8761 aagcatataa aaactatact cctgaaactt ctgtacaagt aatcaaattg gatgcagaag
     8821 ataaagctaa atctgtcctt aaagatgtaa aggctgatgg agctgatttt gcaaagattg
     8881 caaaagaaaa aacaacagct actgataaaa aagttgagta taaatttgat tctgcaggga
     8941 caagcctccc taaagaagtt atgtcagcag cctttaagct agataaaaat ggtgtttcag
     9001 atgtggtttc aacggttgat tcaacaactt ataaaacaag ttactacatc attaaagtaa
     9061 ctgataagac agagaaaaaa tctgattgga aatcttacaa aaatcgctta aaagaagtta
     9121 ttcttaagga taaaacaagc gatagatcct tccaaaataa agtgatttca aaagccttag
     9181 aaaaagctaa cgttaagatt aaagataaag catttgcagg catcttatca caatatgcta
     9241 caacaagtgg ttcatcatca cttaaaaaat agttgaccaa gtcttaaaaa gactgtataa
     9301 ttaatactat tgagaatgaa atttcaacga ttagatacag aaaagtgacg gttgctgcga
     9361 gtcatggata atcagaaaaa gatgctactc aatggggtaa aactaaaaat aaatgagaat
     9421 gacaagttca ttgaatgaag gtggtaccgc ggttttcgcc cttcgtaatg gacttgtctt
     9481 ttgctatttg tcaactttcg aggaggaaga gatatggcta cttttcaaat taaagaaaaa
     9541 atgttttctc taggcggtaa atttactatt acagacctga ctgggcttcc ctgttatcac
     9601 gttgaaggaa gtttgttccc tctacctaag acttttaaag tctttgacga agaggaacat
     9661 ctcattagtc agattgagaa gaaggtttta agttttttac caaagtttaa tgtgacatta
     9721 gccaatggga atcatttcac gattaagaaa gatttctcat ttttgaaacc tcattacacc
     9781 attgaagacc ttgatatgga agttaaggga aatttttggg atatggattt ccaactctta
     9841 aaagacaatc aagttatagc aaacatatct caacagtggt ttcgtatgac ttcaacttat
     9901 caggtagaag tttacagtga aacttacaac gatttgacaa tttcgcttgt catagcgatc
     9961 gactatgtca aagagttaga aaaaaatgca tcaaactaat tttaggagag aataatgaaa
    10021 gaattatcgt ctgcacaaat ccgccaaatg tggttggatt tctggaaatc caaaggacat
    10081 tccgttgagc cttcagctaa cttggttcct gttaatgacc caacgcttct ttggatcaac
    10141 tcaggtgttg caaccttgaa aaaatatttt gatggttcag tgattccaga aaatccacgt
    10201 attaccaatg cacaaaaatc aattcgtact aatgatattg aaaatgttgg taaaacagca
    10261 cgtcaccata ctatgtttga aatgcttggt aacttctcaa ttggagacta tttccgtgat
    10321 gaagctattg agtggggatt tgaactcttg acaagtcctg aatggtttga cttccctaaa
    10381 gacaagctct acatgactta ttacccagat gacaaggatt catataaccg ttggattgct
    10441 tgtggcgttg aaccaagtca cttggtgcca atcgaggata acttctggga aatcggtgct
    10501 ggtccttcag gtccagatac tgagattttc ttcgaccgtg gtgaagattt tgatccagaa
    10561 aatattggac ttcgtctttt ggctgaagat atcgaaaacg atcgttacat cgaaatctgg
    10621 aacattgttc tctcacaatt caatgctgac ccagccgtac cacgttcaga atacaaagaa
    10681 ttgccaaaca aaaacattga tacgggtgcg ggtcttgaac gtcttgcagc tgttatgcaa
    10741 ggggcaaaaa caaactttga aactgacctt ttcatgccta tcatccgtga agtagagaaa
    10801 ttgtcaggta aaacttacga tccagatggc gacaacatga gcttcaaggt tatcgctgat
    10861 cacatccgtg cgctttcatt tgccatcggt gatggtgcgc ttcctggaaa tgaaggtcgt
    10921 ggttatgttc ttcgtcgtct tctccgtcgt gcggttatgc acggtcgccg tcttggcatc
    10981 aacgaaactt tcctttacaa attggttccg actgttggac aaatcatgga aagctactat
    11041 ccagaagtgc ttgaaaaacg tgattttatt gagaaaatcg ttaaacgtga ggaagaaaca
    11101 tttgctcgta ctatcgatgc tggtagcggt cacttagatt cattgcttgc gcagcttaag
    11161 gctgaaggta aagacacact tgaaggtaaa gatatcttca aactttatga tacttatgga
    11221 ttcccagttg aattgacaga ggaattggca gaagatgcag gctataagat tgaccacgaa
    11281 ggctttaagt cagccatgaa agaacaacaa gaccgtgcgc gtgcagctgt tgttaagggt
    11341 ggttcgatgg ggatgcaaaa tgaaacccta gctggtattg ttgaagaatc acgattcgaa
    11401 tacgacacat atagtcttga atcaagtctt tcagttatca ttgctgataa tgaacgtact
    11461 gaggctgttt cagaaggtca agcccttctt gtctttgctc aaacaccatt ctatgctgaa
    11521 atgggtggac aggttgctga cacaggtaga atcaaaaatg ataagggtga cacagttgct
    11581 gaggttgttg atgttcaaaa agcaccaaat ggtcaacctc tacacactgt aaacgttttg
    11641 gcatcacttt cagttggaac aaactacaca cttgaaatca acaaagagcg tcgtttggct
    11701 gttgagaaaa accacacagc aactcacttg ctccatgcag cccttcacaa tgttatcggt
    11761 gaacacgcaa ctcaggctgg ttcattgaac gaagaagaat tcttgcgctt tgattttact
    11821 cactttgaag cagtaagcaa tgaggaactt cgtcacattg aacaagaagt taatgagcaa
    11881 atttggaacg ctcttacaat cacaacgact gaaactgacg ttgaaaccgc aaaagagatg
    11941 ggagcaatgg cactttttgg tgagaaatat ggtaaagtgg ttcgtgtggt tcaaattggt
    12001 aattattctg ttgaactttg cggtggaact cacttaaata attcttcaga aatcggtctc
    12061 ttcaagattg tcaaagaaga aggtattggt tcaggcactc gtcgtattat tgcagttact
    12121 ggtagacaag cttttgaagc ttatcgtaac caagaggatg cactaaaaga gatcgctgct
    12181 actgtaaaag ctccgcaatt gaaagatgca acagctaaag tacaagctct tagcgattca
    12241 cttcgtgatc ttcaaaaaga aaatgtagag cttaaagaaa aagcagcagc agcagcagct
    12301 ggtgatgtct ttaaagatat tcaagaagct aagggcgtgc gcttcattgc tagtcaagtt
    12361 gatgttgcag acgcaggtgc acttcgtaca tttgctgata actggaaaca aaaagactac
    12421 tctgatgtgc ttgttctcgt agcagctatt ggtgagaagg ttaatgtcct tgttgcaagc
    12481 aaaactaaag atgtccacgc tggtaacatg atcaaaggat tggcaccaat tgtagcaggt
    12541 cgtggtggtg gtaaaccaga catggctatg gcaggtggta gcgatgcaag taaaattgca
    12601 gagctgctag cagcagttgc agaaaattta taagaagtac ctctcattta gttagtaaat
    12661 aaaacaggca aagggcagaa catcgttctg ttcctttgtg tatataggag aaatatggtt
    12721 ggtttaggaa cagttatcaa tgttattctt attatagttg gaggctttgt aggtttattt
    12781 ttgaaaaatt ttttgaaaga atcacttcaa aagagcctca tgcaagccat gggagtcgca
    12841 gttttattta tttcaatatc aggtgttttg gagaaaatga tgttagtcga aaagtcacat
    12901 ctcatctcta atcatacaaa tatgatgatt attactcttg ctctaggtac ggtacttgga
    12961 gagttgctat cactagattc gtatattgat agatttgggg actatcttaa gcaaaaaaca
    13021 ggaagtggta acgatattaa atttgtggaa gcattcgtca cctcaacttg tactgtttgt
    13081 attggagcaa tggccgttgt tggtagcatt caagatggta tagcagcaga tcattcgatt
    13141 ttatttgcta agggaatgtt agacatgatt attattgcaa ttatgacagt gtctttaggg
    13201 aaaggggctc tattttctgc acttcctgtc gctttactac aaggtagcct tacgattgtt
    13261 gcttttttca tggggagcct acttaatcca tcatctttag attaccttaa cttagtagga
    13321 aatatgctta tattctgtgt cggtgttaat ttactcttta acctaaatat taaagttatc
    13381 aatatgttgc cagcaattat tttagctata ttatggggaa gttttatata aaagataacc
    13441 aagttttaac tcaaacttgg tttttcttta gacata
//
LOCUS       DATROQ010000053        12967 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.054, whole genome shotgun sequence.
ACCESSION   DATROQ010000053 DATROQ010000000
VERSION     DATROQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12967
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.054"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            134..574
                     /locus_tag="VBC94_001331"
     CDS             134..574
                     /locus_tag="VBC94_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6979601.1"
                     /translation="MENPLQKARILVNQLEKYLDRYAKEYDVEHLAGPQGHLVMHLYK
                     HPDKDMSIKDAEEILHISKSVASNLVKRMEKNGFIAIVPSKTDKRVKYLYLTHLGKQK
                     ATQFEIFLEKLHSTMLAGITKEEIRTTKKVIRTLAKNMAMEDFD"
     gene            621..2438
                     /locus_tag="VBC94_001332"
     CDS             621..2438
                     /locus_tag="VBC94_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979602.1"
                     /translation="MFNIFKRLNGKEWLMIVASTILVCLAVWMDLKTPEYMSDITRML
                     QTKGTKLEALWDPGLKMLAFSFGSFLVAVMVGFLGARTAASFTSRLRSDIFNHVMDYS
                     DAEIKRFSIPSLLTRTTNDLTQLQILIVMGMQVVTRGPIMAIWALTKIGGKSDQWTGA
                     VGVAVIIVMILLSVLLLLAFPRQRQIQGFTDALNATTREALSGVRVIRAYNAEKYQTD
                     KFKRENDDLTKLNLFVYRLMSLMNPVMTVVSSGLTLAIYWIGSYLINDISVPKVTGTS
                     VSVLAAKSAITDRVDLFSNMVVFSSYAMQVVMGFMMMVAIFIIMPRALVSARRINEVL
                     TLAPTVTFKENSHIQSAPKGQIEFQDVSFHYSKDSEAVIEHVSFKADTGQTVAFIGST
                     GSGKSTLVNLVPRFYDATEGSIKLDGIDIKEYSHNDLNNKIGYIPQKAVLFSGTVRSN
                     MIFGESGREELSDEQIWQALELAQAKEFIEAKEKGLDTEVAQGGSNFSGGQKQRLAIA
                     RALARHPEILIFDDSFSALDYKTDHILRHVLKEKTAAMTKLIVAQRISTIMDADLILV
                     LDQGKVVGQGTHKELLSNNEVYQEIAYSQLSKEELENEN"
     gene            2428..4182
                     /locus_tag="VBC94_001333"
     CDS             2428..4182
                     /locus_tag="VBC94_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979603.1"
                     /translation="MKTKSQKTSFFSNLSPYLKGYKLLFALAIVLATLSSLTTVIGPD
                     RLKEMTDLMVKGLAGNIDLAKIGHIAGQLAVLYVLGALVSYLASFIVTTIIQKFAQGM
                     RNAIADKINKIPLSYFDSHTQGDTLSRVTNDVDLMTQSFNQSLVTMVASTVLLVGSLI
                     MMFKTDWHLATTAIISVFSGFALSSIIMVKSQPLFKKQQVNLANISGYVEEIYSGHNI
                     VTSHNARQQAKSDFQLLNDKLHSSMWKSQFFSGIMMPLMQFIGNFGYVMVCVVGAMLA
                     IKGTITMGTIVAFMTYVRIFTQPIGQIAQGITQLQSASAAMGRVFEFLNETNMQEDKH
                     KVLNLPKFKGHVSFEHVKFGYSDNKTIIHDFTSEAQPGQKVAIVGPTGAGKTTIVNLL
                     MRFYDVNSGSISIDDINIQDMKRSQVHDAFSMVLQDTWLFEGTIKENLIFNQTTISDD
                     EVQEAAKAVGIHHFIMTLPKGYDTVLDDAVTLSVGQKQLLTIARALLKNSPLLILDEA
                     TSSVDTRTEELIQKAMDKLMEGRTSFVIAHRLSTIKNADLILVMKDGNIIEQGNHDVL
                     MSQSGFYADLYNSQFESA"
     gene            4290..4916
                     /locus_tag="VBC94_001334"
     CDS             4290..4916
                     /locus_tag="VBC94_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO6979604.1"
                     /translation="METNIYGDYGRYLPLILEDFSQRIQLENDKAKVETGYKLYEHII
                     GRIKTSDSMIEKCRRKQLPVTVDSALKVIRDSIGVRIICGFVNDIYQIVERIKTFDDC
                     RIVVEKDYIQHVKPNGYRSYHVILEIDTPYPDCLGNSEGKYYMEIQLRTIAQDSWASL
                     EHQMKYKHDIENPERIVRELKRCADEIASVDLTMQTIRQLIESGTKKE"
     gene            4918..5604
                     /locus_tag="VBC94_001335"
     CDS             4918..5604
                     /locus_tag="VBC94_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6979605.1"
                     /translation="MRVLIAEDEEQMSRVLSTAISHQGYVVDVAYDGQTAIDLANQNA
                     YDVMVMDVMMPVKTGVEAVKEIRQSGNKSHIIMLTAMAEIDDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMSRRLEDFTPNVLSLGRVTLSVGEQELQCENTIRLAGKEAKMLAFF
                     MLNHDKELSTQQLFEHVWGADKDQEDVDEGYVFIYVSYLRQKLQAIGANLEIIGQEGS
                     SYKLSEINGG"
     gene            5604..6833
                     /locus_tag="VBC94_001336"
     CDS             5604..6833
                     /locus_tag="VBC94_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6979606.1"
                     /translation="MFRNLRLRFIGIAALAILVVLFSVVGVLNSANHYQTKNEIYRVL
                     TILADNNGRIPNKLEFSKELGDDLSTDAIFQFRYFSARTDAKGNVTSFDSRNIFEVSD
                     RQIKNYAKRIVSQNSHSGHITYNFSTYSYLLKKVGKNDYLVVFLDTTNQYLDNQRLLQ
                     LSIWMSLVSFIVFMVIVSVLSGRVILPFVANYEKQRRFITNAGHELKTPLAIISANNE
                     LVEMMSGESEWTKSTNDQIQRLTGLINGMVSLARFEEQPDISMVDLDFSHITKDAAED
                     FKGPIIKDGKDFIMSIQPGIHVKAEEKSLFELVTLLVDNANKYCDPMGTVTVKLSRSS
                     RLRRAKLEVSNTYKNGKDIDYSKFFERFYREDESHNNKKSGYGIGLSIVTSLVHLFKG
                     SIDVNYKHDTITFVIYI"
     gene            6949..7827
                     /gene="mvk"
                     /locus_tag="VBC94_001337"
     CDS             6949..7827
                     /gene="mvk"
                     /locus_tag="VBC94_001337"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEO6979607.1"
                     /translation="MKEKFGIGKAHSKIILMGEHSVVYGYPAIAIPLKNIEVTCLIEE
                     APQLIALDMTDPLSTAIFAALDYLGKTSSKIAYHIESQVPERRGMGSSAAVAIAAIRA
                     VFDYFDEDLEADLLECLVNRAEMIAHSNPSGLDAKTCLSENTIKFIRNIGFSTVPMHL
                     NAYLVIADTGIHGHTKEAVDKVKSSGEAVLPFLKELGYLAEASEDAIHKSDSKQLGSL
                     MTKAHQSLKQLGVSSLEADHLVEVAISCGALGAKMSGGGLGGCIIALVKEKREAERLS
                     QQLEREGAVNTWTEKV"
     gene            7809..8753
                     /gene="mvaD"
                     /locus_tag="VBC94_001338"
     CDS             7809..8753
                     /gene="mvaD"
                     /locus_tag="VBC94_001338"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEO6979608.1"
                     /translation="MDGKSISVKSYANIAIIKYWGKADAEKMIPATSSISLTLENMYT
                     ETRLTALGKDAKKDEFYISGVLQNDHEHDKMSAILDRFRQNRSGFVKIETTNNMPTAA
                     GLSSSSSGLSALVKACNDFFGTNLSQSQLAQEAKFASGSSSRSFFGPVAAWDKDSGDI
                     YKVHTNLDLAMIMLVLNDKRKPISSREGMKICTETSTTFNEWVRQSEQDYQDMLVYLK
                     NNDFQKVGQLTERNALAMHSTTKTATPAFSYLTEETYKAMDVVKKLREKGHECYYTMD
                     AGPNVKVLCLRQDLEALAAILEKDYRIIVSTTKELADG"
     gene            8746..9738
                     /locus_tag="VBC94_001339"
     CDS             8746..9738
                     /locus_tag="VBC94_001339"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEO6979609.1"
                     /translation="MVKVQTGGKLYIAGEYAILYPGQVAILKNVPIYMTALATFADNY
                     SLYSDMFNYTASLQPDKQYSLIQETILLMEEWLINFGKNIKPIHLEITGKLERYGLKF
                     GIGSSGSVVVLTIKAMAALYEIEMPSDLLFKLSAYVLLKRGDNGSMGDIACIAYEHLI
                     SYSAFDRRAVSKMIETKPLEQVLEAEWGYRITKIQALLEMDFLVGWTMQPSISKEMIN
                     IVKSTITQRFLDDTKYQVVQLLSAFKEGDKEAIKRCLEEISLLLFNLHPSIYTDKLQK
                     LKEASKGLDIVTKSSGSGGGDCGIAISFNKNDNQTLIKRWESAGIELLSKETLS"
     gene            9735..10730
                     /locus_tag="VBC94_001340"
     CDS             9735..10730
                     /locus_tag="VBC94_001340"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEO6979610.1"
                     /translation="MTNRKDDHIKYALKYQSHYNSFDDIELIHSSLPKYNVNDIDLST
                     HFAGQSFEFPFYINAMTGGSEKGKAVNHKLAQVAQATGIVMVTGSYSAALKNDEDDSY
                     PTTDLYPDLKLATNIGLDKPVPAAESTVKAMNPIFLQVHVNVMQELLMPEGEREFHMW
                     RSHLKEYVDNIQCPLILKEVGFGMDLQSIKDAYDIGITTVDISGRGGTSFAYIENQRG
                     RDRSYLNTWGQTTAQSLINAQSMMDKMDILASGGIRHPLDMVKCLVLGAKAVGLSRTV
                     LELVERYPVDDVIAILNSWKEDLRMIMCALNCKKITDLRQVNYILYGQLKEANAK"
     gene            10798..11016
                     /locus_tag="VBC94_001341"
     CDS             10798..11016
                     /locus_tag="VBC94_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEO6979611.1"
                     /translation="MIKLYYKDAYDTERCQVFENPNDFIRVLMGCVTVPDNYPITKMT
                     YKDKDIAYSGTYGDLFKTFIEFDWSTLD"
     gene            complement(11062..11916)
                     /locus_tag="VBC94_001342"
     CDS             complement(11062..11916)
                     /locus_tag="VBC94_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO6979612.1"
                     /translation="MTTFTAKFIDQEWEVPVESGRYHMIVGEFCPYAQRPQIARQLLG
                     LDKHISISFVDDVPSDIGLIFSQPEQVTGAKSLRDIYHLTDPTYQGPYTIPILIDKTD
                     NRIVCKESADLLRLFTTDFSDLHQEDAPVLFSQETASLIDNDIKDINKNFQSLMYKLA
                     FLDKQADYDTYSKEFFTFLDQKEHLLGQRPFLLGDNLSEVDIHFFTPLVRWDIAGRDL
                     LLLNQKALEDYPNIFSWAKTLYNDFNLKTLTNPQSIKNNYYLGKFGRAVRHHTIVPTG
                     PNMAKWEK"
     gene            complement(12017..12931)
                     /locus_tag="VBC94_001343"
     CDS             complement(12017..12931)
                     /locus_tag="VBC94_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO6979613.1"
                     /translation="MKSAYIFFNPKSGKDEQALAKEVKSYLIEHDFQDDYVRIITPSS
                     VEEAVALAKKASEDHIDLVIPLGGDGTINKICGGVYAGGAYPTIGLVPAGTVNNFSKA
                     LNIPQERNLALENLLNGHVKSVDICKVNDDYMISSLTLGLLADIAANVTSEMKRKLGP
                     FAFLGDAYRILKRNRSYSITLAYDNNVRSLRTRLLLITMTNSIAGMPAFSPEATIDDG
                     LFRVYTMEHIHFFKLLLHLRQFRKGDFSQAKEIKHFHTNNLTISTFKRKKSAIPKVRI
                     DGDPGDQLPVKVEVIPKALKFIIPNSLP"
BASE COUNT         4228 a         2005 c         2600 g         4134 t
ORIGIN      
        1 tttttatttg acgaatgata ggtgtttttt agaaatatta atgtaaaaat agttctcacg
       61 gtaactttta attgacaatt aataaaggtt gttttaaaat agttctcacg ataactatta
      121 aaggagtatt attatggaga atcctcttca aaaagcacga atacttgtta atcaacttga
      181 aaagtattta gatcgttatg caaaagagta tgatgttgaa catttagcgg gtccacaagg
      241 acacttagtg atgcatctct ataaacatcc tgacaaggat atgtctataa aagatgctga
      301 agaaatttta cacatttcta agtctgtagc gtctaatttg gtaaaacgga tggaaaaaaa
      361 tggatttatt gcgattgttc catctaagac agataagcgt gtgaaatacc tttatttgac
      421 tcatctaggt aaacaaaagg ctacacaatt tgaaattttc ttagaaaaat tgcacagtac
      481 catgttagca ggtattacta aagaagagat acgtactacc aaaaaggtta ttagaacatt
      541 agctaaaaat atggcgatgg aagattttga ttaactattt taggataata ggataaatat
      601 gaaagtaaaa ggagtttgcg atgtttaata ttttcaagcg actcaatggt aaagaatggt
      661 tgatgattgt ggcgagtact atattagtgt gtctagcggt ttggatggac ctaaagactc
      721 cagaatatat gtctgatatc acacgtatgt tgcaaactaa aggtacaaag ttagaggctc
      781 tttgggatcc tggactaaaa atgcttgctt tttcatttgg tagtttttta gtagcagtaa
      841 tggtaggttt tttaggtgct aggacagcag ctagttttac tagtcgatta cgttcagata
      901 tttttaacca tgtgatggat tattctgatg cagaaattaa gcgattttct atcccctctc
      961 tattaacgcg cacaactaac gacttgactc agctacagat ccttattgta atgggaatgc
     1021 aggttgttac ccgtggacca attatggcaa tatgggcctt gactaaaatt gggggtaaaa
     1081 gcgatcaatg gactggggca gttggagtag cggttattat cgttatgata ttacttagcg
     1141 ttctactctt acttgctttt ccgagacaac gtcaaatcca aggttttaca gatgcgctta
     1201 atgcaactac tagagaggca ctctctggtg ttagagttat tcgtgcctac aatgctgaaa
     1261 agtatcaaac agataagttc aaacgtgaaa atgacgattt aacaaaattg aacctttttg
     1321 tttatagatt gatgtcgctt atgaatcctg tgatgacagt ggtttcatct ggattgactt
     1381 tagctattta ttggattggc tcttatttga ttaatgatat ttctgtgcct aaagttacag
     1441 gaacaagtgt atctgtattg gctgctaaat cagctatcac tgatcgagtg gatctttttt
     1501 caaacatggt tgttttttca tcttatgcta tgcaagttgt tatgggattt atgatgatgg
     1561 tagctatttt tatcatcatg cctcgtgctt tagtatcggc tagacgtatt aatgaggtac
     1621 taactttagc cccaacagta acttttaaag aaaatagtca tatccaaagt gcaccaaaag
     1681 gtcaaattga gtttcaagat gtgtcatttc attatagtaa agattctgaa gcggttattg
     1741 aacatgtatc tttcaaagca gatactggtc agacagtggc atttattggg tctacaggtt
     1801 ctggaaaatc cactttagtc aatttagttc cacgttttta tgacgccaca gaggggtcaa
     1861 ttaaacttga tggcattgat ataaaggaat actctcacaa tgatttgaat aataaaattg
     1921 gctatattcc acagaaagct gttctcttta gtgggacagt acgttctaat atgatttttg
     1981 gagagagtgg cagggaggaa cttagtgatg aacaaatttg gcaagcactc gaattagcgc
     2041 aagctaaaga atttatagaa gctaaagaaa aaggattaga tacagaagtc gctcaaggtg
     2101 gtagtaactt ttcaggagga caaaaacaac gcttggcaat cgcacgtgct ttagcacgtc
     2161 accctgaaat tctcattttt gatgactcat tttcagcgtt agattataaa acagatcata
     2221 ttttacgcca tgtgctgaaa gaaaaaacag ctgctatgac gaaattaatt gtcgctcaac
     2281 gcatttcaac tatcatggat gctgatctca ttttagtgct agatcaaggt aaagttgttg
     2341 ggcaaggaac acataaggaa ttgttatcaa ataatgaagt atatcaagag atagcctatt
     2401 ctcaattgtc taaggaggaa ttggaaaatg aaaactaaat cacaaaaaac gtcttttttc
     2461 tctaatttat ctccgtacct caaaggttac aaacttcttt ttgcgttagc aattgtgtta
     2521 gcaactttat caagcttgac aacagttata ggaccagacc gtcttaaaga gatgacggat
     2581 cttatggtaa aaggcttggc tggtaatatt gacttagcca aaatcggtca tattgcaggg
     2641 caattggcag tattatatgt tttaggtgca ttagtatcct atctagcaag ttttattgtg
     2701 acgactatta ttcaaaaatt tgctcaaggt atgcgcaatg ctattgctga taaaatcaat
     2761 aaaatacctt tgagttattt tgatagccat actcaaggtg atactttatc tcgtgttaca
     2821 aatgatgtgg atttgatgac acagtctttt aaccaaagtt tagttactat ggttgcatct
     2881 acagtgttat tggttggttc attaattatg atgttcaaaa cagattggca tttagcaaca
     2941 acggcgatta tatcggtatt ttcaggattc gctctttctt caatcattat ggtgaagagt
     3001 cagcctttat ttaaaaagca acaggtgaat ttggctaata tatctggata tgtagaagaa
     3061 atttactcag gacacaacat tgttactagt cataatgcaa gacagcaggc taagtcagat
     3121 tttcaactat taaatgataa acttcacagt tccatgtgga aatcacaatt tttctctgga
     3181 attatgatgc ctctgatgca gtttattggt aattttggtt atgtgatggt gtgtgtggtt
     3241 ggtgccatgt tagctatcaa aggaacaatt actatgggta caattgttgc ttttatgacc
     3301 tatgttcgta tttttacaca gcctattggt cagattgctc aaggaattac acaattacag
     3361 tctgctagtg cagccatggg acgtgttttt gaatttttaa atgaaacaaa tatgcaagag
     3421 gataaacaca aagtgttaaa tcttcctaag tttaaggggc atgtgtcctt tgaacatgtc
     3481 aagtttggtt atagtgataa caagactatt attcatgact ttacttcgga ggcacagcct
     3541 ggtcagaaag ttgctattgt aggaccaact ggcgcaggta aaacgacaat tgttaattta
     3601 ttgatgcgtt tttatgatgt taattcaggt agtatttcca tagatgacat caatatccaa
     3661 gacatgaaac gtagtcaagt tcatgatgct ttctcgatgg ttcttcaaga tacatggttg
     3721 tttgagggaa ctattaaaga aaatttgatt tttaaccaga cgacaatcag tgatgatgag
     3781 gttcaagaag ctgctaaggc agtcggtata catcatttta taatgacact acctaaaggg
     3841 tatgatactg tccttgatga cgctgtgaca ttgtcagttg gacaaaagca attattaacc
     3901 attgctcgag ctcttttgaa aaattctccc ttacttatct tagatgaagc aacttcatct
     3961 gttgatacac gtacagaaga gttaattcaa aaagctatgg acaaacttat ggaaggaaga
     4021 acatcatttg ttatcgcaca tcgtttatca acgattaaaa acgcagactt gattttggtc
     4081 atgaaagatg ggaatatcat tgagcaaggc aatcatgatg ttttgatgtc tcaaagtggt
     4141 ttttatgctg atctgtataa ttctcaattc gaatcagctt aacataaaca ttttagaagg
     4201 aaatgggaag aaaaaattct cattcctttt tagtttgcct taagtttcta tgatataatg
     4261 gagcttaaga gagaatagga aggtttctta tggaaacaaa tatttatgga gattatggtc
     4321 gatatctacc attaatttta gaagatttca gtcagcgtat ccaattagaa aatgataaag
     4381 ctaaggttga aacaggctat aaattgtacg aacatatcat tggtcgtatt aagacatcag
     4441 atagtatgat agagaaatgt cgtcgtaaac agttacctgt aacagtagat tctgcactaa
     4501 aagttattag agatagtatt ggagtccgta ttatttgtgg ttttgttaac gacatttatc
     4561 aaattgtaga acgtattaag acatttgatg attgtcgtat tgtagttgaa aaagactata
     4621 tccagcatgt taagccaaat gggtatcgct cttaccatgt gattttagaa attgatactc
     4681 cctatccgga ctgtttgggt aattcagaag gtaaatatta catggagatt cagttgcgta
     4741 caattgcgca agattcttgg gctagtttag aacatcagat gaaatacaag catgatattg
     4801 aaaatcccga acgaattgta agagaattaa aacgttgtgc tgatgaaata gcatctgttg
     4861 atttaacgat gcaaacgatt cgtcaattga tagagagtgg aacaaagaag gaataaaatg
     4921 agagtattaa ttgcagaaga tgaagaacag atgtcacgtg tccttagtac ggcaattagt
     4981 catcaaggtt atgttgttga tgtcgcctac gatggtcaga ctgctattga tttagctaat
     5041 caaaatgcat atgatgtcat ggttatggat gtcatgatgc ctgttaaaac aggtgttgag
     5101 gctgtcaaag aaattagaca atcaggaaat aaatctcaca ttattatgct aacagccatg
     5161 gcagaaattg atgatagagt aacaggtcta gatgctggtg cagatgatta tttaacaaaa
     5221 cccttctctc taaaagaatt attagcaagg cttcgctcta tgtcccgaag attggaagat
     5281 ttcacaccta atgtattgtc attgggaaga gtaaccttat cagtcggtga acaagagttg
     5341 caatgtgaaa acactattcg tctagcgggt aaagaagcta aaatgttagc atttttcatg
     5401 ttgaaccatg ataaagaatt atctacacaa caacttttcg aacatgtctg gggagcagat
     5461 aaggaccaag aagatgtaga tgagggatac gtctttatct atgtttctta tttgagacaa
     5521 aaattgcagg caattggtgc taacttggag attattggtc aagaaggtag tagctataaa
     5581 ttaagtgaaa taaatggtgg ctaatgttta gaaatttacg acttcgtttt atcggaatag
     5641 ctgcgctagc tattcttgtt gtcttatttt cagtagtcgg tgtcctcaac tctgctaatc
     5701 actatcaaac gaagaatgaa atttatcgtg tgttaactat ccttgcagac aacaatggac
     5761 gtataccgaa taaacttgaa tttagtaaag aattaggtga tgacctatca acggacgcta
     5821 ttttccaatt ccgttatttc agtgcgcgaa cagatgcaaa aggtaacgtg actagttttg
     5881 attctagaaa tatctttgaa gtaagcgata ggcaaataaa aaattatgcc aagcgtatcg
     5941 tatcacaaaa tagtcattca ggtcatatca cctataattt tagtacttat tcttatctac
     6001 taaaaaaggt tggtaaaaat gattatttag tggtattttt agatacaacc aaccaatatc
     6061 ttgataatca aagattattg caactttcaa tatggatgtc cttggtaagt tttatcgtct
     6121 tcatggtaat tgtttcagtt ttatcaggga gagttatctt accatttgtt gctaactatg
     6181 aaaagcaaag gcgttttatt accaatgcag gacatgaact aaaaactcca cttgctatca
     6241 tctccgctaa taatgaattg gtggaaatga tgagtggtga atcagagtgg actaaatcta
     6301 caaatgatca aatccaacgt ttaacgggtc taattaatgg catggtcagt cttgcccgct
     6361 ttgaagaaca gcctgatata agtatggttg acttagactt ttcacatatt acaaaagatg
     6421 ctgcagaaga ttttaaaggt ccaatcatta aagatggtaa agactttata atgagtatcc
     6481 aaccaggtat tcatgtcaaa gctgaagaaa aatcattatt tgaattggtt acattattgg
     6541 ttgataatgc taataagtat tgtgatccta tggggactgt gacagttaag ttatcacgtt
     6601 caagtcgttt gagacgagct aagcttgaag tttcaaatac ttataagaat ggtaaagaca
     6661 tagactatag caagtttttt gaacgatttt atcgagagga tgagtcacat aataacaaaa
     6721 aatcgggata tggtattggt ctttctatcg taactagttt ggtgcatcta tttaaaggaa
     6781 gtatcgatgt gaattataaa catgatacca ttacctttgt catttacatt taagattaaa
     6841 gggactgcaa ttgtagttcc tttaatttat gttattatca cgttaaagtc gcttttttta
     6901 aggatttata ttaaaatagt aaggtaatag aattgtaaag agaaaaagat gaaagaaaag
     6961 tttggtatag gaaaggctca tagcaagatt atcttgatgg gtgagcactc agtagtatat
     7021 ggttatccag ccattgctat tcctttaaaa aatattgaag taacttgcct tattgaagag
     7081 gctcctcaat taatagcatt agatatgact gatcctttgt cgacagccat ttttgctgcc
     7141 ttggactatt taggtaaaac ttcttctaaa attgcatatc atattgagtc acaagtccca
     7201 gaaagacgtg gtatgggatc atcagctgca gttgctattg cagctattcg tgcagttttt
     7261 gattattttg atgaagattt ggaggcagat ttgttagaat gccttgttaa tcgagcagaa
     7321 atgatagcac attcaaatcc gagtggatta gatgcaaaaa cttgtcttag tgaaaatact
     7381 attaaattta tccgcaatat tggtttttca acagtaccca tgcatttgaa tgcttattta
     7441 gttattgctg atacaggaat tcatgggcat actaaagaag cagtggataa ggtgaaatca
     7501 tctggcgagg cagtactacc ctttttaaaa gaattaggtt atttagcaga ggcatcagag
     7561 gatgctattc ataaaagtga ttccaaacaa cttggtagct tgatgactaa agcacatcaa
     7621 agtttaaaac aattgggtgt ctcaagttta gaagctgatc atctggtaga ggtagctatt
     7681 agttgtggtg ctctaggcgc taaaatgagt ggcggcggat taggtggttg tattatcgca
     7741 cttgtaaaag aaaaaagaga agctgagcgc ttaagtcagc aattagaaag agaaggagct
     7801 gtcaacacat ggacggaaaa agtataagtg ttaagtctta tgctaacatt gctattatca
     7861 aatattgggg aaaagcagat gctgaaaaaa tgattccagc taccagtagt atttctctaa
     7921 cacttgaaaa catgtacact gaaacacgtt tgactgccct tgggaaggat gctaagaaag
     7981 atgaatttta catctctggt gttcttcaaa atgatcatga acatgacaaa atgtcagcta
     8041 tcttggatcg ctttcgacaa aataggtccg gctttgtaaa gattgaaaca actaataata
     8101 tgccaacagc tgcaggctta tcatcgtcat caagtggttt atcagcctta gtaaaggcct
     8161 gcaatgattt ttttggtact aaccttagcc aatcacagct agctcaagaa gccaagtttg
     8221 cttcaggctc ctcttcacgc tctttttttg gacctgttgc tgcttgggat aaagatagtg
     8281 gtgatatcta taaagttcac actaatttag atttagctat gattatgctg gtcttaaatg
     8341 acaaaagaaa gccgatttca agccgtgaag gtatgaaaat ctgcaccgaa acttcaacta
     8401 cttttaatga atgggtaagg caatctgaac aagattatca agatatgtta gtatacctaa
     8461 aaaataatga ttttcaaaag gtaggtcaac tgacagagcg taatgcactt gcaatgcact
     8521 caacaactaa aacagcaact ccagcctttt catatttgac agaagagaca tataaagcta
     8581 tggatgttgt gaagaaactt cgcgaaaaag gtcatgaatg ttattatacg atggatgcgg
     8641 gtccaaatgt taaagtactt tgcttacgtc aagaccttga ggcattagct gctatcttgg
     8701 agaaagatta tcgtattatt gtttcaacga caaaggaatt agcagatggt taaggtgcaa
     8761 acaggtggca aattatatat tgcaggtgaa tacgcgatac tgtatcctgg gcaagtagct
     8821 attttaaaga acgttcctat ttatatgact gcattagcta cttttgcaga taactacagc
     8881 ctttattctg atatgtttaa ctatacggca agcttacagc cagataagca atatagtctc
     8941 attcaggaaa cgatattgct tatggaagaa tggttgatta attttggcaa aaatataaaa
     9001 ccgattcacc tcgaaattac gggaaaatta gagcgctatg gacttaaatt tggtataggc
     9061 tctagcggaa gtgttgtcgt tttgactatt aaagctatgg ctgcgcttta tgagatagag
     9121 atgccaagcg acttgctttt taaattatct gcctatgttc tcttaaaaag aggtgataat
     9181 ggctcaatgg gagatattgc ttgtattgct tatgagcatt taatttctta ttcagctttt
     9241 gatcgtaggg ctgtttcaaa aatgattgaa acaaaacctt tggaacaagt attggaagct
     9301 gaatggggtt atcgtattac caaaatacaa gctttattgg agatggattt tttagtggga
     9361 tggacaatgc aaccgtccat ttcaaaagaa atgataaata tagttaaatc aactattacg
     9421 cagcgttttt tagatgatac aaagtatcaa gttgtccagt tactttcagc ctttaaagaa
     9481 ggagataaag aagcgattaa gagatgttta gaagaaatta gcttgctatt atttaatctt
     9541 catccatcta tttacaccga taaacttcaa aagcttaaag aggcaagcaa aggcctagat
     9601 atagtcacaa aatcttcagg atctggagga ggcgattgtg gtatagccat atcctttaat
     9661 aaaaatgata atcaaactct aatcaagcgt tgggagagtg ctggtattga actacttagt
     9721 aaggagacac tgtcatgaca aatcgaaaag atgatcacat aaaatatgct cttaaatatc
     9781 agtcgcatta taatagtttt gacgatattg agttaataca ttcatcctta ccaaaatata
     9841 atgtcaacga tatcgattta tcaacacatt ttgctggtca aagctttgaa tttccttttt
     9901 atatcaatgc tatgacaggc ggtagtgaaa aagggaaagc agtcaatcat aaattggcac
     9961 aggttgctca agctacaggg attgttatgg tgacaggttc atattcggca gctctcaaaa
    10021 acgatgaaga tgattcctac ccaacaacag acttgtatcc tgatttaaaa ctggcgacaa
    10081 atatagggtt agacaagcct gtaccagcag cagagtcaac cgttaaggct atgaacccca
    10141 tttttttaca agttcatgtt aatgtgatgc aagaactttt gatgcctgaa ggagagcgtg
    10201 aatttcacat gtggcgttct cacttaaaag aatacgtcga caatatccaa tgcccactta
    10261 ttttaaaaga ggttggtttt gggatggatt tacaatcaat aaaggatgct tacgatatag
    10321 gtatcacaac tgtagatatt tcggggcgtg gggggactag ttttgcttac atcgaaaatc
    10381 aacgtggacg cgaccgttcg tatctcaata cctgggggca aacaacagct caatccttaa
    10441 taaacgctca atctatgatg gacaaaatgg atatcctagc atctggtggc attcgtcatc
    10501 ctttagatat ggtaaaatgt ttagtgttgg gtgctaaagc tgttggactt tctagaacag
    10561 ttttagaatt agttgagcgt tatcctgtag atgatgttat tgcaattctc aattcttgga
    10621 aagaagattt aagaatgata atgtgtgcac tgaattgtaa aaaaataact gatttacgac
    10681 aagtcaatta tatattgtat ggtcaattga aggaagccaa tgctaaatga tattattttt
    10741 gtgaagtaga tatagcttcc tgtcaaagtt tccttgtaaa acgtcagagg taataagatg
    10801 attaaactgt attataaaga tgcttacgac actgaacgat gccaagtttt tgaaaatcca
    10861 aatgatttta ttcgagtttt aatgggatgt gttactgttc ctgacaatta tcctattacg
    10921 aaaatgactt ataaagataa agatattgct tacagtggta cttatggaga tctcttcaag
    10981 acttttattg aatttgattg gtccactttg gactagtata gctaaaaagc taaggctgtg
    11041 aattgcctta gctttttagc tttatttttc ccattttgcc atatttggac ctgttgggac
    11101 gatggtatga tgacgaacag ctcttccaaa tttgcctaag taatagttat tttttatgga
    11161 ttgtggattg gttaatgttt ttaaattaaa gtcattgtat aaagttttag cccaagaaaa
    11221 aatatttggg tagtcttcta gcgctttctg attaagtagt agaagatcac gcccagcaat
    11281 atcccatcgg actaatggtg taaagaaatg aatatcaacc tcacttagat tatctccgag
    11341 caagaaagga cgttgtccta gtaagtgttc tttttgatct agaaaagtga agaattcctt
    11401 actataagta tcataatctg cttgtttatc aagaaaagct aacttataca ttaagctttg
    11461 gaagttctta ttaatgtctt taatgtcatt atcaataagt gaagcagttt cttggctgaa
    11521 aaggacagga gcatcctctt gatgcaaatc agagaaatct gtagtaaaaa gtcgaagcaa
    11581 atctgcagat tccttacaga caatacgatt atcggtcttg tcaattaaaa ttgggatagt
    11641 ataaggacct tggtaggtcg ggtcagtcaa atgataaata tcacgaagcg acttagcacc
    11701 agtgacctgt tctggttggc taaaaataag tcctatatca cttggaacat catccacaaa
    11761 actgatagaa atatgtttat ccaaaccaag cagttggcgc gcaatctgcg gacgttgtgc
    11821 ataaggacaa aattccccaa caatcatatg gtagcgacct gattctactg ggacttccca
    11881 ctcttggtca ataaatttag cagtaaatgt tgtcatcgta tacttccttt caatgatatt
    11941 tatcgcttta agtataacaa aaaagaacct agacagatag tatttactct aatacttatg
    12001 tctaggtcct tttatattat ggtaatgagt taggaatgat aaactttaag gctttaggga
    12061 tgacttcaac ttttacaggt aattggtcac ctggatcgcc atcaatacga actttaggga
    12121 tagctgattt tttacgttta aaagttgaaa tagtcagatt attggtatga aaatgtttta
    12181 tctcctttgc ttgactaaaa tctcccttac gaaattgtct tagatgcaac aataatttga
    12241 agaagtgaat atgttccata gtgtagactc taaaaagtcc atcatcaatt gttgcttctg
    12301 gagagaaggc tggcataccg gcaatcgaat tggtcatggt aataaggagt aaccgcgtgc
    12361 gtaaagaccg tacattatta tcgtaagcaa gagtgataga atatgatcga ttacgcttta
    12421 atatacggta ggcatcacct aagaaagcaa aagggcctaa tttacgcttc atttcagaag
    12481 tgacattagc agcaatatca gcaaggagac caagtgttaa actgctaatc atgtaatcgt
    12541 cattaacttt acagatatct acggacttta catgaccgtt tagaaggttt tcaagagcga
    12601 gattcctctc ttgcgggatg ttcaaagctt ttgaaaagtt attgacggtc ccggcaggta
    12661 ctagtccgat agtaggataa gcaccaccag cgtagacacc accacagatt ttattaatag
    12721 tgccatctcc ccctaaagga ataaccaagt cgatatggtc ttcagatgct ttttttgcta
    12781 aggcgacagc ttcctcaaca gatgaaggtg taataatacg aacataatcg tcttgaaaat
    12841 catgttctat taaataagat tttacttctt ttgccaatgc ttgttcatct ttaccagatt
    12901 ttggattaaa aaagatataa gcacttttca tatgtccacg ccttctctct aagatttaga
    12961 tgattat
//
LOCUS       DATROQ010000054        12831 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.055, whole genome shotgun sequence.
ACCESSION   DATROQ010000054 DATROQ010000000
VERSION     DATROQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12831
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.055"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..842)
                     /locus_tag="VBC94_001344"
     CDS             complement(<1..842)
                     /locus_tag="VBC94_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6979614.1"
                     /translation="MKTRRTPLEKKVKYIISVWAKKFGLLHTLKSISREKYAEKISAS
                     LLYGILSSVAVNFFFQPGHVYSSGATGLAQVISAVSKHWFSFEIPVALAFYAINIPLL
                     ILSWRKIGHKFTIFTFITVTVSSIFIQLMPQITLTTDPLINAIFGGLIMGAGVGFSFK
                     SRISSGGTDIISLTIRKKTGRDVGSISFIINGIILLFAGLLFGWKYALYSMVTIFVSS
                     RVTDAIFTKQKKMQAMIVTSKPDCVIKRIHRDLHRGVTCINDAEGTYNHEKKAVLITI
                     LTRE"
     gene            complement(832..2583)
                     /gene="aspS"
                     /locus_tag="VBC94_001345"
     CDS             complement(832..2583)
                     /gene="aspS"
                     /locus_tag="VBC94_001345"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEO6979615.1"
                     /translation="MKRSMYAGRVRSEHIGTSITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEEVAASVMATAESLRSEFVIEVSGVVTAREQANDNLPTGEVELKVQELSV
                     LNTSKTTPFEIKDGIEANDDTRMRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDNLEF
                     IDVETPMLTKSTPEGARDYLVPSRVNQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSDQEIQDIVEGMIAKVMKDTKGLEVSLPFPRMAY
                     DDAMNNYGSDKPDTRFDMLLQDLTEIVKEVDFKVFSEASVVKAIVVKDKADKYSRKNI
                     DKLTEIAKQYGAKGLAWLKYVDNTISGPVAKFLTAIEGRLTEALQLENNDLILFVADS
                     LEVANETLGALRTRIAKELELIDYSKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     AETAHELEGDLAKVRAVAYDIVLNGYELGGGSLRINQKDTQERMFKALGFSAESAQEQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SEQQLEELSLTVESYEN"
     gene            complement(2676..3956)
                     /locus_tag="VBC94_001346"
     CDS             complement(2676..3956)
                     /locus_tag="VBC94_001346"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEO6979616.1"
                     /translation="MKLQKPKGTQDILPGESAKWQYVENVIRNLFKQYHYDEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFHDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PTKMYYIGSMFRYERPQAGRLREFHQVGVECFGSNNPTTDVETIAMGHHLFEDLGIKN
                     VKLHLNSLGSPESRQAYRQALIDYLTPIREQLSKDSQRRLNENPLRVLDSKEPEDKLA
                     VENAPSILDYLDESSQAHFDAVCHMLDALNIPYIIDTNMVRGLDYYNHTIFEFITEIE
                     DNELTICAGGRYDGLVSYFGGPETPAFGFGLGLERLLLILDKQGISLPIENTIDLYIA
                     VLGSEANLAALDLAQSIRHQGFKVERDYLGRKIKAQFKSADTFNAKVIMTLGSSEVDS
                     KEVGLKNNQTRQEVKVSFENIKTDFSSVLKQLGL"
     gene            4176..4358
                     /gene="rpmF"
                     /locus_tag="VBC94_001347"
     CDS             4176..4358
                     /gene="rpmF"
                     /locus_tag="VBC94_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEO6979617.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            4374..4523
                     /gene="rpmG"
                     /locus_tag="VBC94_001348"
     CDS             4374..4523
                     /gene="rpmG"
                     /locus_tag="VBC94_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6979618.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            4803..5429
                     /locus_tag="VBC94_001349"
     CDS             4803..5429
                     /locus_tag="VBC94_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEO6979619.1"
                     /translation="MRYFMIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIY
                     LGQFLGSVSLIFLSLLFAFVLNYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKD
                     GLRKDNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVTLLTFLVMIYLLVFS
                     AQKLAQVPSVGETLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
     gene            5441..5779
                     /gene="cadX"
                     /locus_tag="VBC94_001350"
     CDS             5441..5779
                     /gene="cadX"
                     /locus_tag="VBC94_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEO6979620.1"
                     /translation="MKKDSICQVDVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLANQNILDTRREGKIIYYFIKDE
                     EIRDFFNQLG"
     gene            6128..7243
                     /locus_tag="VBC94_001351"
     CDS             6128..7243
                     /locus_tag="VBC94_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO6979621.1"
                     /translation="MSDDLISIQSSCLSIAKTFANFNIQRKRFMISTETEMDTFHKKD
                     DIDVWVGGKSYNPARSFRTRTINELTVIDFEEMFDILWLMLGDNLIKSFEVNVCGILF
                     ELGGNDIPSTFRQENIDPLINKWWYDNVSTEIIPNLIKKLKENPLFNIGFMVNDILER
                     MYKENIPKSYLTSVPLVISQKGRTTYSFSMTGGQQIDGVKFTQIYEDYMKLLSQGKDI
                     AELYQKYSKEELANLGINIYQSNDIERTEERTFDEIISWVSNPYATRPIQERHTIQLE
                     PTRFSLEDKKRIEEAAAQGLSEIDLIDLVDLYDINLDNTSVNRHIVGLLTNNTQVTYY
                     FQEQLNKELLSMAHALDNVQQAFIKLLSEEEIRKFAL"
     gene            7563..8012
                     /locus_tag="VBC94_001352"
     CDS             7563..8012
                     /locus_tag="VBC94_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6979622.1"
                     /translation="MSNKVQERRERKIKEAIKAKNWDEVTRLLQQEQSNAERRDRYHN
                     RRIKDETIASKNAKKSVRYDVIASSDLNPEEALILEELRQAIREAKASLSEIDSKIVE
                     MIAEQGSSYKETARYITEHYKKMSDVTVKSHYCKALKKLAPLLKAYR"
     gene            8214..8534
                     /locus_tag="VBC94_001353"
     CDS             8214..8534
                     /locus_tag="VBC94_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979623.1"
                     /translation="MAFKMKTTKGGYTTTLADKIISQKQPIYSLSTELEPQQRFEEGK
                     PTGEIVAYKAWFVQEGQDPFQVKFEETIELPAFQSMIQFDTLQACEVKYNIYFKANGI
                     KEVR"
     gene            8534..9634
                     /locus_tag="VBC94_001354"
     CDS             8534..9634
                     /locus_tag="VBC94_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6979624.1"
                     /translation="MTSQSRDVLNQSYLQSYLLQSLNMALGALMQGETSYTNSFNIVI
                     QADGFIFVPRLPCAYILDDDLYKKIFLIANASLYPQYTLLKQNATYFVPLETDDLHTQ
                     RGLFFPWKKGISERLVIPDLDKFSASLPQGKIPIMKYFELNLDKVNHWAIAGNSGSGK
                     SYALTYFLSVLKHMSDLIIIDPKFDTPSRWARENHVAVIHPVENRSKSDFVSQVNEQL
                     SQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQISLLG
                     RATKIHLFLVSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPIG
                     HGTGIIQVIDNEHPYQVLPLLCPTYYTKRGIL"
     gene            9631..10236
                     /locus_tag="VBC94_001355"
     CDS             9631..10236
                     /locus_tag="VBC94_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979625.1"
                     /translation="MTNKTYFMRSFNLILYLFLFAPICSAFGWLFNYLSVQLTNITFV
                     NLDTVKSITDIISSVCHGFALISLLLALFLVGLEIIQRLKTDSLWNYIQSVYHTFLLR
                     HFLFQRERVQKISNLEHQTVTTINPIHNGFNRAVRKCIVDIRKDTITIFLKVPRDQQG
                     QKILKEMEAQLKEEIVSQHADYYFSSPIRVRNQLWFTGQKR"
     gene            10442..11047
                     /locus_tag="VBC94_001356"
     CDS             10442..11047
                     /locus_tag="VBC94_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEO6979626.1"
                     /translation="MAKEQRSTKWTFLFYEESAPENYLNILEELHIPFILSPWHDKDV
                     NRQTGEFKKSHKHGAFFFDSLKSYSQVSNIISDKLNGPAHVEVVMSPTGLFDYFTHAE
                     NPDKTPYNIEDIEVGCGFNLEKFLMEMNSSDFIHEVVDIIEENDFTEFEELVWYARAN
                     NTNLLGLIIERTYFFAKYLDSRRYNPNRLHNSNTEEKENNE"
     gene            11040..11288
                     /locus_tag="VBC94_001357"
     CDS             11040..11288
                     /locus_tag="VBC94_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6979627.1"
                     /translation="MNNEKLEQIEQLLQTLIKLIQIKEQNIPLKIIQQRELLKQLNIS
                     PNTLKTWEQKGLKRLEPPIEGTRTVFYLLDDIINFLQS"
     gene            11378..12643
                     /locus_tag="VBC94_001358"
     CDS             11378..12643
                     /locus_tag="VBC94_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6979628.1"
                     /translation="METETIIHNGKKVIKKIKKDKSISYTLKGTFLGKDIKTGKQVTT
                     TITAKTLKQLDRAIIQARLEFEKNGSTREKVIVIDTLEDLAEEWFKSYKTWVNSHNTL
                     NRVRGYLDNYIIPKFGDYKPDKIESADIQLWLNDLAKKSKESIESGIKRADKGSAKDF
                     GAVIHKLKDIFDYGITNYGLITNPVSTVKIPPKPKSNKQRIMVLHDDGLVIWLNYLES
                     LDNNRANRRFKLICNTLLASALRINELLALTIDDLDFENSSINVSKTLMWKTANKKDG
                     TKGEVICKATPKTDAGNRSVPVPPPIIEKLRDFHHEMNKYLELHNRHRTKLIFPTIYG
                     NYMCDRNERTTLKKRLVGLGLPNYGFHLFRHTHASMLLNAGTNWKELQVRMGHKSIST
                     TMDTYAELAPQRKLEAVGIYLEKIAELTQ"
BASE COUNT         4357 a         2396 c         2123 g         3955 t
ORIGIN      
        1 tcttcacgtg ttaatatagt tattaaaact gcttttttct catgattata agtaccttca
       61 gcatcattga tacaggttac accacgatgt aaatcacgat ggatacgttt aatgacgcaa
      121 tcaggcttac tggtaacaat catcgcttgc atcttttttt gctttgtaaa aatggcatct
      181 gtaactctac ttgatacaaa aattgtcacc atcgagtaca aagcgtactt ccaaccaaat
      241 aaaagacctg caaataataa aataatacca ttaataataa aagaaatact acctacatct
      301 ctgcctgttt tctttctaat tgtcaaactg ataatatctg ttcccccact agaaattcta
      361 gacttaaagc taaaaccaac accagctccc ataattaagc caccaaagat agcattgatt
      421 aagggatcag ttgtaagggt gatttgaggc attagttgaa tgaagataga gcttacagtt
      481 actgtgataa acgtaaaaat agtaaattta tgtccaatct tacgccaaga taatatcagc
      541 aaagggatat taatggcata aaaagctaaa gcaactggta tttcaaaaga aaaccagtgt
      601 ttactgacag ctgaaataac ctgagctaaa ccagttgctc cacttgaata aacatgtcca
      661 ggttgaaaaa agaaattaac agcaacactg gatagaatac catacagtaa tgaagccgaa
      721 attttctctg catatttttc tcgtgagata ctttttaaag tgtgtaagag accaaatttt
      781 ttagcccata cactgataat atattttacc tttttttcta aaggggttcg tctagttttc
      841 ataactttct acagtaagtg ataattcttc taattgttgt tctgaaacga gactaggagc
      901 ttgagtcatt ggatcactag ccttattgtt tttagggaat gcaataactt ctcgaatatt
      961 gtccttgcct gctaacaaca taacaaagcg gtcaaggcct attgccaatc caccatgtgg
     1021 tgggaagccg taatccatag cttcaagtaa gaaaccaaac tgttcctgag ctgattcagc
     1081 gctaaatcct aaagccttga acatacgctc ttgtgtatct ttttggttaa tacgtaggct
     1141 accaccgcct aactcatatc cattcaaaac aatatcatag gcaactgcac gtactttagc
     1201 caaatcccct tccaactcat gcgctgtctc cgctgttggc aaggtgaatg gatgatgagc
     1261 cgacatatat cgtccctctt cttctgacca ctcaaacatt ggccaatcaa caacccatag
     1321 gaaattaaat ttagaataat caatgagttc aagttctttt gcaatgcgtg tacgaagcgc
     1381 tcctaaagtc tcgttagcta cttcaagact atctgctaca aatagaatta agtcattatt
     1441 ctctagctgc aaagcttctg tcaacctacc ttcaatagct gtcaagaact tagcaaccgg
     1501 acctgaaata gtattatcta catattttag ccatgctaaa cctttggcac cgtattgttt
     1561 tgcaatttct gttaatttat caatattttt tcgagagtac ttgtccgcct tgtctttgac
     1621 aacaatcgct ttaactactg aagcttctga gaacaccttg aagtcaactt cttttacaat
     1681 ttctgtcaag tcttgtaaaa gcatatcaaa ccttgtatca ggcttatcag acccgtagtt
     1741 attcattgca tcatcatatg ccatccttgg gaatggtaaa gaaacctcta aacctttagt
     1801 gtctttcatt actttagcaa tcataccctc aacaatgtct tgaatttctt gatcactaag
     1861 gaatgatgtc tctaagtcaa cttgtgtaaa ctcaggttga cgatctccac gtaaatcctc
     1921 atcacgaaaa catttgacaa tttgataata acgatcaaat cctgcattca tcaatagctg
     1981 ttttgtaatt tgagggcttt gtggaagggc ataaaaatgt ccttggttga cacgtgatgg
     2041 aaccaaatag tctcgtgccc cctctggagt tgattttgtt agcatcggag tttcgacatc
     2101 aataaactct aagttgtcaa ggtaattacg gattgagtgt gttactttcg cacgtaattt
     2161 gaaattttca agcatttccg gacgacgaag atccaagtaa cggtaacgca tgcgtgtatc
     2221 atcatttgcc tcaataccat ctttaatttc aaatggtgtt gttttcgagg tattaagaac
     2281 cgataattct tgcactttta attcaacttc tcctgttggt aaattatcat tagcttgttc
     2341 tcgtgctgtc actacaccgc taacctctat aacaaattca ctacggagac tttctgcggt
     2401 tgccataact gaggctgcta cttcttcagg attaataact aattgcatga ttccctcgcg
     2461 atcgcgaaga tcaataaaaa taaggcctcc aaggtcacga cgacgaccaa cccatccttt
     2521 taaagtaatc gatgttccta tgtgctcact acgaacacgc ccagcataca tactacgttt
     2581 catctatttt ctccatttaa aatattcatt ccaacctatt ataacaaaaa gcacctaaaa
     2641 accccaccca ttaaggagag tttttaggaa ctgttttaca atcctaattg tttaagcaca
     2701 gatgaaaaat ctgttttaat gttttcaaaa ctaactttaa cttcttgacg tgtttgatta
     2761 ttttttaaac cgacctcttt cgaatctact tcactgctac ctaatgtcat aatcacttta
     2821 gcattaaaag tatctgcaga cttaaattgt gctttgattt tacgacccaa ataatcacgc
     2881 tctactttaa aaccttgatg acgtattgat tgtgccaaat ctaaggcagc tagattagcc
     2941 tctgatccta atacagctat atacagatct atagtatttt ctattggtaa agaaatccct
     3001 tgcttatcta atattaagag caagcgttct aatcctaatc caaaaccaaa tgctggtgtt
     3061 tctggtccac caaaatagga tactaagccg tcataacggc ctccagcaca gatcgttaat
     3121 tcattatctt caatctcagt gataaactca aaaatagtat ggttataata atcaagacca
     3181 cgaaccatat tagtatcaat gatgtatgga atatttagag catcaagcat atgacacaca
     3241 gcatcaaagt gggcttggct tgactcatct aaataatcta aaatagaagg agcattttca
     3301 actgctagtt tgtcttctgg ctccttagag tctagtacgc gcaatggatt ttcattcaaa
     3361 cgacgttgac tatccttaga caattgttca cgtatgggtg ttaagtaatc aataagagct
     3421 tggcgatagg cttgacgact ttctggactt cctaaactat taagatgaag tttaacgttt
     3481 ttgataccta aatcttcaaa tagatgatgt cccattgcga ttgtttcaac atcagttgtt
     3541 ggattatttg atccaaagca ctcaacgcct acttgatgaa attctcgtaa acgaccagcc
     3601 tgtgggcgct catatcggaa cattgatcca atataataca ttttggttgg tttttgaact
     3661 tctggtgcaa atagtttatt ttcaacataa gaacgtacaa caggtgctgt tccttctgga
     3721 cgaagcgtaa tgtgacgatc tcctttatca tgaaaatcat acatctcttt tgtaacgata
     3781 tccgttgtat ccccaactga gcgactaata acctcgtaat gttcaaacat tggtgtacga
     3841 atttcatcat aatgatattg tttaaagaga ttgcgtataa cattttcaac gtattgccac
     3901 tttgctgatt ctccaggtaa gatatcctga gtcccttttg gtttttgaag tttcataagt
     3961 ttacctcttc gattagtttt ctctttaaac attctaccat tttttcttat attttaaaca
     4021 agcatatatc atttgcttta gtttgatagt attttcacca tgaaattgta aaaataaaaa
     4081 gttttgtcaa gtaactttac tttacaaaaa agatatgtta tcatagtgaa gttgatgaaa
     4141 atcaaaactt acacatcgaa aggaagaata agaaaatggc agtacctgca cgtcacactt
     4201 caaaagcgaa gaaaaacaaa cgtcgtacac actacaaatt gacagctcca tctgtacaat
     4261 tcgacgaaac tactggagat tactcacgtt cacaccgtgt atcactcaaa ggatactaca
     4321 aaggacgtaa aatcgctaaa gctaacgaag ctaaataata gaagggagat accatgcgcg
     4381 taaatattac acttgaacat aaagaatctg gtgaacgctt gtaccttact tcaaaaaaca
     4441 aacgtaacac tccagaccgt cttcaattga aaaaatactc accaaaactt cgtaaacacg
     4501 ttgttttcac tgaggttaag taagggtttc agtacacgtc aatagacgtc aaaatattga
     4561 ttttaagcga tttttgattt tttgaaactc attatatcgc actacaaatc aataaaaact
     4621 ctacctttta ctctaccttt tttagataaa tatatcatct ggatattgaa gttaagccct
     4681 gctatcattt cgatggcagg gctttttaat atttgtacac taacttctta tctatatttt
     4741 ggtggatttt aagttgacat ctacaaagtt taatgttaga atacattcaa aaatatattt
     4801 gaatgaggta ttttatgatt caaaatgttg ttacttcaat aatcctgtat tctgggacag
     4861 ccgtagactt acttattatc ctaatgttat tttttgccaa aagaaaaagc agaaaagaca
     4921 tcattaacat ctatttagga caatttctag gctctgttag tctaatattc ctaagtttgc
     4981 tttttgcatt tgtcttaaat tatattccta gtaaagagat tttaggttta ctcggtttga
     5041 ttccaatttt cctaggccta aaagttttgc ttttaggaga ttctgatgga gaagctattg
     5101 caaaagatgg tttgcgaaaa gacaataaaa acctgatttt tctagtcgct atgattactt
     5161 ttgcaagttg tggcgctgac aatattggtg tctttgtccc atattttacc accttaaatt
     5221 tagcgaattt gatagtgact ttacttactt ttctagtcat gatttatctc ttggtttttt
     5281 ctgcacaaaa attagcacaa gtcccttctg ttggagaaac tttggaaaaa tatagcagat
     5341 ggtttattgc cgttgtctat ttaggattgg ggatgtatat cctgattgaa aacaacagct
     5401 ttgacatgct atgggctgtg ttaggctagg agaaaaaatt atgaaaaaag atagtatctg
     5461 tcaagtggat gttataaatc aacaaaatgt tacaaccgca acgaactacc ttgaaaagga
     5521 aaaagtccaa aaatcacttc gcattttatc aaaatttacc gataataaac agataaatat
     5581 catcttttat ctccttgccg tcgaagaact ctgtgtctgc gatatagcct gtttattaaa
     5641 tctcagtatg gcatctgcct cccaccatct tcgtaaacta gccaatcaaa acatcttgga
     5701 cactagaaga gaggggaaaa ttatatatta ttttataaaa gatgaggaaa tcagagattt
     5761 ttttaatcaa ctaggataac aactatttct actactttct acttttccat gattataggg
     5821 ggattatata tgaaattttg aagaaaatta ttcacaagaa atacctactc gtatcaaaaa
     5881 tttaagaaaa aaatacaata ttacacaaag cgaactaggc aatgcaggtc aagttagtca
     5941 agttgaaagt ggtaagcgac caattacgag ttcgatgtta gtttatttaa atgctctaac
     6001 cgcttcgagc tatacgtata tcgtttttgg agagttagat gagtttattg agaatctgtt
     6061 tcattatttt ttcagttcta ttttatatag agatttagag gctgtagatg aaaaactata
     6121 ttctttcatg agtgatgatt taatttcaat ccaatcaagt tgtctaagta ttgccaagac
     6181 atttgcaaat ttcaatattc aaagaaaaag atttatgatt tctactgaaa ctgaaatgga
     6241 tacttttcac aagaaagatg acattgacgt atgggttggt ggaaaaagtt ataaccccgc
     6301 tagaagtttt agaacccgta ccatcaatga attgactgtc attgattttg aagaaatgtt
     6361 tgatattctt tggttgatgt taggggataa tttaattaaa tcatttgaag ttaatgtctg
     6421 tggtatccta tttgaattag gcggaaatga cataccctca acatttaggc aagaaaacat
     6481 tgatcctctc atcaataaat ggtggtatga taatgtttcg acagaaatta tccctaatct
     6541 aataaaaaaa ctcaaagaaa atcccttgtt taatatcgga tttatggtaa atgatatatt
     6601 agaaagaatg tataaagaga atattccaaa atcttacctt acatccgtcc cattagttat
     6661 ttctcaaaaa ggaagaacaa cctattcgtt tagtatgact ggtggtcaac aaatagatgg
     6721 agtgaaattc acacagatat atgaggacta tatgaaatta ctcagtcaag gtaaggatat
     6781 cgcagagtta tatcaaaaat attctaaaga agagttggca aatctaggca ttaatattta
     6841 tcaatccaat gatatagaaa ggactgagga aagaactttt gatgaaatta tcagttgggt
     6901 ttccaaccct tatgcaacaa gaccaattca agaaaggcac actattcaat tagagccaac
     6961 aagattttca ctagaggata agaaaagaat tgaagaagct gcagctcaag gactaagcga
     7021 aatcgacctt attgatttag ttgacctata tgatattaat ttagacaata caagcgtcaa
     7081 tcgccatatt gtggggttat tgactaataa cacccaagta acatactatt tccaagaaca
     7141 attaaataag gagttgctgt caatggctca cgctttagat aacgtacaac aggcctttat
     7201 taaattatta agtgaagagg agatacgaaa atttgctctt taaagactag gtgaatcgcc
     7261 tagtcttttt tgcgcctcgt tttacaaatt agtctattga agtaaaaaaa gaagtaaaaa
     7321 ataaaagaac attacgcctt atactgaata tatcaagaaa aactcttgat ataaatttta
     7381 atagtccaaa ttgatggcca tcaaaggaca gattgaaaca aatatgatga catgacgata
     7441 aaggtcatgt cttgcataga attgtttcgc tgtttctttg ttgtgccttt tggtatactc
     7501 agcgttcaat atttgctcat tttgtttcct tctgtccaaa ggacagaaag gagctcatca
     7561 ttatgagtaa taaagtacaa gaacgtcgtg agcgtaaaat caaagaagct attaaagcta
     7621 aaaattggga tgaagtaact cgtttgcttc aacaagaaca aagtaacgca gaacgtcgtg
     7681 accgatacca caatagacga atcaaagacg aaacaatagc tagcaaaaat gccaaaaagt
     7741 ctgtacgtta tgatgtcatt gcaagttctg atttgaatcc cgaagaagct ttaatcctag
     7801 aggagttaag acaagctatt cgtgaagcta aagcatccct atcagaaatt gacagtaaga
     7861 ttgttgaaat gatagcggaa caaggttcaa gctataaaga aacggctcgc tatatcactg
     7921 aacattataa gaaaatgagt gatgtaactg ttaaatccca ttattgtaaa gcgctaaaaa
     7981 aattagctcc tttattaaaa gcttaccgct aatccctcaa atccagtgtc tctgtcactg
     8041 gattttttgt aaaaaaaaat taaaaattca acaaataact ttgcgtttag ctgtctcatt
     8101 ctccttagta atgaggaggt ggtcagataa cctaaaaaac aaagtccagc tcatgaccca
     8161 aaccgagcct gtcgcattca cactatctct tattcataag gaggatttgc cctatggcgt
     8221 ttaaaatgaa aaccacaaaa ggtggttata caactacatt ggctgataaa attatcagtc
     8281 aaaaacaacc gatttactca cttagtaccg aacttgaacc acaacaacgg tttgaagaag
     8341 gaaaaccaac cggagaaatc gtagcttata aagcatggtt tgtacaggaa gggcaagacc
     8401 ctttccaagt aaaatttgaa gaaacgattg agcttccagc ttttcaatcc atgattcaat
     8461 ttgacacact acaagcctgc gaagtaaaat ataacatcta cttcaaagca aatggtatca
     8521 aggaggttcg gtaatgactt cacaatctcg tgatgtgctg aaccaatctt acctgcaatc
     8581 ttacctgctc cagagcctaa acatggctct gggggcttta atgcagggag aaaccagcta
     8641 caccaattct tttaatattg ttattcaagc agacggcttt atctttgtcc ctcgtctacc
     8701 atgtgcttat atccttgatg atgacttgta caagaaaatc tttctgattg ccaatgcttc
     8761 actttatcca caatatacgc ttctaaaaca aaacgctacc tattttgtcc ctctagaaac
     8821 agatgactta cacactcagc gtggattatt tttcccttgg aaaaagggaa tttcagaacg
     8881 tttagtcatt ccagatttgg ataagttttc agctagcttg ccacagggga aaatccctat
     8941 catgaagtac tttgaactca atttagataa ggttaatcac tgggctattg ctggaaattc
     9001 aggttctggg aaatcgtacg ctctcactta tttcttgagt gtactaaagc atatgtctga
     9061 cttgattatc attgacccta aatttgatac cccaagtcga tgggcaagag aaaatcacgt
     9121 tgctgttata caccctgttg aaaaccgttc aaaatcagat tttgtctcac aggtcaatga
     9181 acaattaagt cagtgtgcaa ctctcattca gaaacgacaa gctatcttgt atgataatcc
     9241 caatcatcaa tttacccatc taactattgt tattgatgaa gtcctcgctc tatcagaggg
     9301 agtcaataag aatatcaaag aagccttttt ctccctactc tcacagattt ccttattagg
     9361 acgtgctacc aaaatccatc tgtttttggt aagccagcgt tttgaccaca atactattcc
     9421 aatttcagtg agggaacagc taaacgtatt gttgcaaatt ggaaatatca atcagaaaac
     9481 tactcaattt ttatttccag acctagaccc agagggtatt gttattccaa taggacatgg
     9541 gacaggtatc attcaagtta tcgataatga acatccctac caagttctgc ccttactctg
     9601 tccaacctac tacactaaac gaggtattct atgaccaata aaacctactt tatgagaagt
     9661 tttaatctca tactatattt atttctattt gcacctatct gttctgcatt tggatggtta
     9721 ttcaattatc tttccgttca attaacaaat atcacgtttg tcaatttaga tacggtcaaa
     9781 agtattacgg atatcattag ttccgtttgt catggatttg cacttatttc tcttctactt
     9841 gccttatttt tggttggatt agaaataata caacggttga aaacagatag tctttggaac
     9901 tatatccaat ctgtctatca cacttttctg ttgcgacact tcctgtttca gcgtgaacga
     9961 gtacagaaga taagcaatct tgaacatcag acggttacta ctatcaaccc aatccataac
    10021 ggatttaacc gagctgttcg taagtgtatc gtagatatac gaaaagatac catcactatt
    10081 tttctaaaag tccctagaga ccaacaaggg caaaaaatcc taaaagagat ggaagcacaa
    10141 ttaaaagaag aaatcgtcag ccaacacgca gactattatt tctcctctcc tattcgagtt
    10201 cgtaaccaat tgtggttcac tggacaaaaa cgttaatccc ttggggctgt ggcttgcgtt
    10261 aagcaaaagc cagacagccc ctttttatat tcttttctat catgacctat tggggtgact
    10321 acgaccccca ataggtcaat aatcaataat cacaaaaaat tcaatttttt caaaaaaact
    10381 ttgcatacga cctgtcattt ctccttagta atagaaccca aaagaaatga ggtaactgcc
    10441 tatggcaaaa gaacagcgct caaccaaatg gacttttctc ttctacgagg aaagcgcccc
    10501 agagaactac ttgaatatat tagaggaact ccatattccg tttattctta gtccttggca
    10561 cgataaggac gttaatagac agacgggaga gttcaagaaa tcgcacaaac acggtgcttt
    10621 cttctttgat tcactgaaaa gctattcaca agtatcaaat atcatcagtg acaaactaaa
    10681 cggtccagca catgtggagg tagttatgtc tcccactggt ttatttgact actttaccca
    10741 tgcggaaaat ccagataaga ccccctacaa cattgaggat attgaagtcg gttgcggctt
    10801 taatttagaa aaattcttga tggagatgaa ctcctcggat tttatacatg aagtagttga
    10861 tattatcgaa gagaacgact ttaccgaatt tgaagaattg gtctggtatg cccgagcaaa
    10921 caataccaat ttattagggc ttatcattga acgtacatat ttcttcgcta aatacctaga
    10981 ttcacgaaga tacaatccta atcgactcca taattcaaac acagaggaga aagagaataa
    11041 tgaataacga aaaattagaa caaattgaac aattattgca aacacttatc aaactcattc
    11101 aaatcaaaga acagaacata ccactaaaga taatacaaca gcgagaattg ctaaaacagc
    11161 taaatatctc cccaaacaca ctaaaaactt gggaacaaaa aggattaaaa agattagaac
    11221 ctcctataga gggaactcga accgtcttct atctactaga tgatattatc aactttttac
    11281 aatcctaatt ctgaaaattt gtgctaaaat agttttatct aaaagaaaga gtggtaacta
    11341 ttttagccta cttcaatatc catgaagaaa ggcaaatatg gaaactgaaa caattattca
    11401 taatggcaaa aaagtcatta aaaaaatcaa aaaagataag tccatctcat acactttgaa
    11461 ggggacattt ctgggaaaag atatcaaaac tggtaagcaa gtcactacta ccattacagc
    11521 caagacacta aaacagttag atagagcaat cattcaagca cgactagaat ttgaaaaaaa
    11581 tggctcaaca cgagaaaaag ttatcgtcat tgatacactg gaagatttag ctgaagaatg
    11641 gtttaaatca tataaaacct gggttaactc gcataatact ctgaacagag taagaggtta
    11701 tcttgataat tatattatcc ctaaatttgg agattacaaa ccagataaaa ttgaatctgc
    11761 ggatatccag ctttggttaa acgatttggc aaagaaatca aaagagtcca ttgaatctgg
    11821 tataaagcgt gctgataaag gtagtgctaa agattttggt gctgttatcc ataagttgaa
    11881 agatattttt gactatggaa ttacaaacta tggactaatc actaatcctg ttagcactgt
    11941 taagatacca cctaagccaa aatcaaataa gcaacgcatc atggtattac atgatgatgg
    12001 tcttgtaata tggcttaatt atcttgaaag tttagataac aatagagcca atcgtagatt
    12061 taagttaatt tgtaatactc tattagcctc cgctttgcga ataaatgaat tacttgcatt
    12121 gacgattgat gacctagatt ttgaaaattc ttctattaac gtcagtaaaa cattgatgtg
    12181 gaaaaccgca aataaaaaag atgggacaaa aggggaagtt atctgtaaag ctacaccaaa
    12241 aactgatgca ggaaatcgct ctgtccctgt ccctcccccc ataatagaaa aactcagaga
    12301 tttccaccac gaaatgaaca agtatcttga attgcacaac agacatagaa caaaactgat
    12361 tttccctaca atctatggga actatatgtg tgacagaaac gagagaacaa ctcttaagaa
    12421 aaggttagta ggtcttggat taccaaacta tggtttccat ttattccgac atactcatgc
    12481 ttcaatgcta cttaatgctg gaacaaattg gaaagaactc caagtcagaa tgggacataa
    12541 atccatttca actacaatgg atacttacgc tgaattagca ccacaacgaa agttagaagc
    12601 tgtggggatt tatcttgaaa agattgctga gctaacgcaa taactactct accattcact
    12661 ctacctttta ttatgtagca ttctagaaac attgatgtac caacgtttct aacagctaaa
    12721 atagaaatta tttaactctt actgaagtta aataattgat tataaaaaag cctatgaaat
    12781 caatatttct gggcttttta tttttaaaaa taaaatctga caaaaaagta t
//
LOCUS       DATROQ010000055        12349 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.056, whole genome shotgun sequence.
ACCESSION   DATROQ010000055 DATROQ010000000
VERSION     DATROQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12349
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.056"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..464)
                     /locus_tag="VBC94_001359"
     CDS             complement(<1..464)
                     /locus_tag="VBC94_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6979629.1"
                     /translation="MIAKEFESFLLNHLDHYLIPAEDVAIFVDTHNADHVMLLLASNG
                     FSRVPVITKEKKYVGTISISDIMGYQSKGQLTDWEMAQTDIVEMVNTKIEPINEAATL
                     TAIMHKIVDYPFLPVISDQNDFRGIITRKSILKAINSLLHDFTDEYTITPKN"
     gene            complement(461..982)
                     /locus_tag="VBC94_001360"
     CDS             complement(461..982)
                     /locus_tag="VBC94_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO6979630.1"
                     /translation="MAIRKLVVMSDSHGDRDIVKDIKNHYLGKVDAIFHNGDSELPSS
                     DPIWEGIHVVTGNCDYDSGYPEVLVTKIDNAVIVQTHGHLHQINFTWDKLDLLAQQED
                     ADICLYGHLHRADAWKNGKTIFINPGSVLQPRGPINEKLYAVVTITDSKVLVEYYTRQ
                     HQPYPNLTKEFSR"
     gene            complement(964..1938)
                     /locus_tag="VBC94_001361"
     CDS             complement(964..1938)
                     /locus_tag="VBC94_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEO6979631.1"
                     /translation="MTKTIFESKTEGNWFLGSFQAFNYFTCFGNDESYEAIQDVFHRL
                     LSTLKVEGLQLHVVQMTSDFQLLAFLVDMINQEYSRHIKVTQHKGAILVSEDDQLFLV
                     HLPKEGTSLEKFFDLKNDNNFGDTILIATHNEGKTKEFRELFGKLGLKVENLNDYPDL
                     PEVEETGMTFEENARLKAETISKLTGKMVISDDSGLKVDALGGLPGVWSARFSGPDAT
                     DARNNAKLLHELAMVFDKERRSAQFHTTLVVSAPNKESLVVEAEWPGYIGTEPKGENG
                     FGYDPLFIVGEGSRTAAELSAQEKNNLSHRGQAVRKLMEVFPKWQLEN"
     gene            complement(1935..2729)
                     /gene="racE"
                     /locus_tag="VBC94_001362"
     CDS             complement(1935..2729)
                     /gene="racE"
                     /locus_tag="VBC94_001362"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEO6979632.1"
                     /translation="MDSRPIGFLDSGVGGLTVVKEMFRQLPEEEVIFIGDQARAPYGP
                     RPAQQIREFTWQMVNFLLTKNVKMIVIACNTATAVAWQEIKEKLDIPVLGVILPGASA
                     AIKSTNLGKVGIIGTPMTVKSDAYRQKIQALSPNTAVVSLACPKFVPIVESNQMSSSL
                     AKKVVYETLSPLVGKLDTLILGCTHYPLLRPIIQNVMGAEVKLIDSGAETVRDISVLL
                     NYFEINHNWQNKHGGHHFYTTASPKGFKEIAEQWLSQEINVERIVL"
     gene            complement(2904..3143)
                     /locus_tag="VBC94_001363"
     CDS             complement(2904..3143)
                     /locus_tag="VBC94_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEO6979633.1"
                     /translation="MSTTIWILLIIVALFGGLVGGIFIARKQIEKEIGEHPRLTPDAI
                     REMMSQMGQKPSEAKVQQTYRNIVKHAKTAIKTKK"
     gene            complement(3301..3843)
                     /locus_tag="VBC94_001364"
     CDS             complement(3301..3843)
                     /locus_tag="VBC94_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979634.1"
                     /translation="MSKKTTQMVSYTSILVAFAIMIPIIMPAKIIIGPASFTLASHVP
                     LFLSIFISVPVAILVALGTGLGFLLAGFPIVIVLRALSHIGFALIAAFLIKSKPSLLM
                     SKWQTLLFAVAINIIHGLLEFITVYIITMTSNSSSTYLWSLFSLIGLGSLLHGLVDFY
                     IALFIWKWMTQKLGKTLRIR"
     gene            complement(3905..4426)
                     /locus_tag="VBC94_001365"
     CDS             complement(3905..4426)
                     /locus_tag="VBC94_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEO6979635.1"
                     /translation="MKAQERRENILTTLKGTKEAISASTLAKIFSVSRQVIVGDIALL
                     RAQQCDIISTPKGYLMSSALSTHQFTARLVCQHGIEQTEEELEIILRYQGIIMNVEVE
                     HPIYGMLTAPLNIQSQKDIDNFTAKLKVSNAELLSSLTDGLHTHMISCQDQSVFDQIC
                     EALKKAGILYIDK"
     gene            complement(4494..5183)
                     /locus_tag="VBC94_001366"
     CDS             complement(4494..5183)
                     /locus_tag="VBC94_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEO6979636.1"
                     /translation="MNDNVIYTQSDSGLNQFFAKIYGLVGIGVGLSAAVSAIMLYMFP
                     QNMIAIMQKMPGLYFGAIILELVLVFVASGAARRNTPAALPLFLIYSALNGFTLSFII
                     ARYTQTTVLQAFITSAAVFFAMALIGAKTKKDLSGMRKALMAALIGILIASLVNLFIG
                     SGGMSYIMSIVCVIIFSGLIAYDNQMIKYVYNSQGGQVADGWAVSMALSLYLDFINLF
                     LNILRLFARND"
     gene            complement(5206..5709)
                     /locus_tag="VBC94_001367"
     CDS             complement(5206..5709)
                     /locus_tag="VBC94_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6979637.1"
                     /translation="MKKYQDDKDFMDLVGHLIDHPRFQKLEAIVQHHHSTRLEHSINV
                     SYTSYKIAKKFGWDASSTARGGLLHDFFYYDWRVTKFNKSHAWVHPRIAVRNARKLTD
                     LNAREEDIILKHMWGATIAPPRYKESYIVTMVDKYWAVREASRPLKRIFKKPIRFSRK
                     FLGSHNH"
     gene            complement(5747..6490)
                     /locus_tag="VBC94_001368"
     CDS             complement(5747..6490)
                     /locus_tag="VBC94_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEO6979638.1"
                     /translation="MTTIITSKSNNLIKKTKKLLQKKYRKSSYLIEGWHLFEEAEKYG
                     AQFLNIFVTETAIDRLRKPERAIVVTDDVLKELTDSQTPQGIVAEIAFQETRWTDIKK
                     GRFLVLEDVQDPGNLGTMVRTADAANFDAVFLSQKSADLYNQKTLRSMQGSHFHLPVF
                     RVEIEQFVNFCKAEGITMIATTLSEQSVNYKNLPKYDYFALIMGNEGQGISKTMTEEA
                     DVLAHIEMPGQAESLNVAVAAGVVIFSLI"
     gene            6522..6800
                     /locus_tag="VBC94_001369"
     CDS             6522..6800
                     /locus_tag="VBC94_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEO6979639.1"
                     /translation="MKKVHLIVSGRVQGVGFRYATYSLALEIGDIYGRVWNNDDGTVE
                     ILAQSTDSNKMTQFIQKIRKGPSKWSKVTYVDIKLDNFDDFNDFKMSN"
     gene            6885..7817
                     /gene="yidC"
                     /locus_tag="VBC94_001370"
     CDS             6885..7817
                     /gene="yidC"
                     /locus_tag="VBC94_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO6979640.1"
                     /translation="MKKTLKRILFSSLSLSMLLLLTGCVSVDKAGKPYGVIWNTLGVP
                     MANLITYFAQHQGLGFGVAIIIVTVIVRVVILPLGLYQSWKASYQAEKMAYFKPLFEP
                     INERLRNAKTQEEKLAAQTELMTAQRENGLSMFGGIGCLPLLIQMPFFSAIFFAARYT
                     PGVSSATFLGLNLGQKSLILTVIIAILYFVQSWLSMQGVPDEQRQQMKTMMYLMPIMM
                     VFMSISLPASVALYWFIGGIFSIIQQLVTTYVLKPKLRRKVEEEYTKNPPKAYKANNA
                     RKDVTNSTKATESNQAIITSKKTNRNAGKQKRRG"
     gene            complement(8017..8682)
                     /locus_tag="VBC94_001371"
     CDS             complement(8017..8682)
                     /locus_tag="VBC94_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6979641.1"
                     /translation="MINWDAIFNLELAVKAFPSVIQGLPYTIGLSLVGFILGAIVGFF
                     VALMKMSHFRLLRYLANIHISLMRGIPLMVLLFLIYFGLPFIGIQLDAVTASIVGFTM
                     MSSAYISEIIRAALLAVDHGQWEAARALGLKTPTIYRGIIIPQATRIALPSLSNVLLD
                     MVKGSSLTAMITVPDIFNNAKIVGGTYSDYMTAYILVALIYWVICTLYAIIQDWWEKR
                     LLY"
     gene            complement(8706..9563)
                     /locus_tag="VBC94_001372"
     CDS             complement(8706..9563)
                     /locus_tag="VBC94_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6979642.1"
                     /translation="MKKTILLGLVGLSAMTLAACSNGQSNKETTWDNIKKDGVLKVAT
                     PATLYPTSYYDDHKKLTGYEIDMMKAIAKKLKIKVKFVEVGVAESFTSVDSGKVDVAV
                     NNFDTTPERLKKYNFSQPYKYSVGGMIVRADGSSKITAKDLSDWKGKKAGGGAGTQYM
                     KIAKQQGAEPVIYDNVTNDVYLRDVSTGRTDFIPNDYYTQVIAVKYVTKQYPDIKVKM
                     GDVKYNPTEQGIVMSKKDKSLKTKIDAAIKDMKKDGSLKKISEKYYAGQDLTKEPAGT
                     DKIPVIKVK"
     gene            complement(9724..11184)
                     /locus_tag="VBC94_001373"
     CDS             complement(9724..11184)
                     /locus_tag="VBC94_001373"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEO6979643.1"
                     /translation="MVFKDATAMVQAIKQHKISSQELVEQAIYKIEEQNVSVNAVVSK
                     QYNEARQAAKYANESNAPFAGVPILLKDLGQNQKGQLSTSGSQLFKHYHAKQTDYLVQ
                     SFEKLGFIILGRTNTPEFGFKNISDGQLHGNVNLPFDHSRNAGGSSGGAAAAVSSGMV
                     PIAGASDGGGSIRIPASFNGLIGLKPSRGRIPVGPSSYRGWQGASSHFALTKSVRDTK
                     RLLYYLQSYQVESPFPLKKLSKESLFEFSVSKPLKIAVLMDSPLKTKVSSEAKAAIKE
                     AADFLSQKGNHLELVEQPLDGIHSMKTYCMMNSVETAAMFDDIEKSLGRSMEFSDMEL
                     MTWAMYQSGQRVLAKDYSKLLDSWDQFAATMARFHENYDLILTAATNQPAPFHGQFDL
                     DETLQKQLRHMGEFSVSEQQDLIWKMFEDSMAWTPFTHQPNLTGQPSLAIPTHLTKEG
                     LPLGVQLTAAKGREDLLLAVAELFEKEKQFKGPVYH"
     gene            complement(11288..11770)
                     /gene="greA"
                     /locus_tag="VBC94_001374"
     CDS             complement(11288..11770)
                     /gene="greA"
                     /locus_tag="VBC94_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEO6979644.1"
                     /translation="MAEKTYPMTQVEKDQLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQIQILETKIRYAEIIDSDAVAKDEVAIGKTVLVQEVGTND
                     KDTYHIVGAAGADIFSGKISNESPIAHALIGKKTGDLATIESPAGSYQVEIISVEKTN
                     "
     gene            complement(11843..>12349)
                     /gene="mltG"
                     /locus_tag="VBC94_001375"
     CDS             complement(11843..>12349)
                     /gene="mltG"
                     /locus_tag="VBC94_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO6979645.1"
                     /translation="TTLEGLVEDMISTMNTKIAPYYNTIKAKNMSVNDVLTLSSLVEK
                     EGSTDEDRRKIASVFYNRLSAGQALQSNIAILYAMGKLGDKTSLAEDAQINTSIKSPY
                     NIYTNTGLMPGPVDSPSISAIEATIKPASTDYLYFVADVKTGNVYYAKDFETHKANVE
                     KYINSQIN"
BASE COUNT         3936 a         2423 c         1943 g         4047 t
ORIGIN      
        1 ttgtttttag gagtgatagt atattcgtct gtaaaatcat gtaggaggct attaatagct
       61 tttaaaatcg atttacgggt tataatgcct ctaaaatcat tttgatcact gataacaggt
      121 aaaaatggat aatctacaat cttatgcata attgctgtca gagtagctgc ctcattaatc
      181 ggctcgattt ttgtattaac catctcaaca atatcagtct gtgccatctc ccaatctgtt
      241 aattgacctt tagattggta gcccataatg tctgagatac taatagtacc tacatatttt
      301 ttctctttag tgataactgg tacacgtgaa aaaccattac ttgctaatag taacataacg
      361 tggtctgcat tgtgtgtatc cacaaaaata gcaacgtctt cagctggaat tagatagtgg
      421 tccaaatgat ttagcaaaaa cgactcaaat tctttagcaa tcatcttgaa aattcctttg
      481 tcaagttagg atagggttgg tgttggcgag tataatactc taccaaaacc ttactatctg
      541 ttatagttac aacagcgtaa agcttctcat ttattggtcc tcttggctgc aaaacactac
      601 ctggattgat aaaaatagtt ttaccatttt tccaagcatc tgcacgatga agatgaccat
      661 ataaacaaat atccgcgtct tcttgctgag ctaataaatc caatttatcc caggtaaaat
      721 taatctgatg gagatgacca tgggtttgaa caataacagc attatctatt tttgtaacca
      781 aaacttccgg ataacctgaa tcataatcac aatttcctgt gaccacatga atgccttccc
      841 atataggatc tgagctagga agttcggaat ccccattgtg aaaaatagca tccacttttc
      901 ccaaataatg attctttata tcttttacaa tgtcacgatc tccgtgagaa tcactcatta
      961 caactaattt tctaattgcc attttggaaa tacctccatc aatttacgga cagcttgccc
     1021 acgatgtgat agattatttt tttcttgtgc gctcaattca gctgctgtcc tactaccttc
     1081 tccgacaata aagagcggat cataaccaaa gccattttcc cccttaggtt ccgtaccaat
     1141 ataacctggc cactctgctt caactaccaa actttcttta tttggagctg acacgactaa
     1201 ggttgtatga aactgtgctg aacgtctttc tttgtcaaat accatggcta attcatgcaa
     1261 taatttggca ttattacgag catctgtcgc gtcaggacct gaaaaacgag cagaccatac
     1321 ccctggcaaa ccacctaaag catctacttt taatccggaa tcatctgaaa ttaccatttt
     1381 tccagttaat tttgaaatag tctctgcttt aagtcgagca ttttcttcaa aggtcatacc
     1441 agtctcttca acttctggta aatctgggta atcatttaaa ttttcaactt tgagtcctaa
     1501 tttcccaaat aattctctaa attccttcgt ttttccttca ttgtgagtcg caattaatat
     1561 ggtatcccca aaattattat cattctttaa atcaaagaat ttttctaaag aagtgccctc
     1621 tttaggtaag tgaactaaga acaactgatc atcttcacta actaagatgg cccctttatg
     1681 ttgagtcact ttgatatgcc ttgaatactc ttgattaatc atatcaacga ggaatgctaa
     1741 taattgaaaa tccgaagtca tctgcacaac gtgaagctgt agaccttcaa ctttcaaagt
     1801 tgataataga cgatgaaaaa catcttggat agcttcgtaa gattcatcat taccaaaaca
     1861 agtaaaataa ttaaaagctt ggaaactacc taaaaaccaa ttgccttcag ttttactttc
     1921 aaaaattgtt tttgtcataa tacgatacgc tccacattta tttcttgact aagccattgt
     1981 tctgcaattt ctttaaaacc ttttgggctg gcggttgtgt aaaagtgatg accaccgtgt
     2041 ttattttgcc aattatggtt tatctcaaaa tagttcaata aaacagaaat atcacgaacg
     2101 gtttctgcgc cactatcaat taatttaacc tcagccccca taacattttg aatgatggga
     2161 cgtaataggg gataatgcgt gcaacctaaa attaaagtat ctaatttacc aactaatggg
     2221 gacaacgttt cataaaccac ctttttggct aaactagaag acatctgatt tgattccaca
     2281 attggaacaa atttcggaca agcaagggat accacagcag tatttggaga caaagcttga
     2341 attttttgac gataagcatc tgatttaaca gtcatgggag tacctataat accaactttc
     2401 cctaaattag ttgatttgat agctgcgcta gctcctggta aaataacgcc taaaacaggg
     2461 atgtctagtt tttctttaat ttcttgccag gcaactgcag ttgctgtatt acaagctata
     2521 acaatcatct taacattttt agtcaataag aagttaacca tctgccaggt aaactctcta
     2581 atctgttgag caggtctagg accatacgga gctctagcct gatctccaat gaagattact
     2641 tcctcttctg gaagttgacg gaacatttcc ttaacaaccg ttaaaccacc tacaccagaa
     2701 tctagaaagc caataggcct actatccatt tttattcctc caatattagt gactaaaaga
     2761 ggctggacaa cagtcctagc ctctctatac aataaccaat cagtcaaatc tacacttgac
     2821 caagtttgcc caaatcttgt ctagaaatct gatggactat cactcactat ttcatcacta
     2881 aacgcttcaa tgctaaaaca ctcttatttt ttagttttga tagctgtttt tgcgtgtttt
     2941 acaatattac ggtatgtttg ttgaacttta gcttcactag gtttttgtcc catttgactc
     3001 atcatttcac gaatagcatc cggtgttaaa cgagggtgct ctccaatctc tttttcaatt
     3061 tgtttacgag caataaaaat gcctcccacg agaccaccaa acaaagcgac gataattaat
     3121 aaaatccaaa tagttgttga catctattca ttctcctcat ttatttacat gactcattat
     3181 agcaaaaaaa gaaagatttt acaaatatat gtaaacaaaa tcaaagcctc tatcgtcctt
     3241 gtcacgtcaa tacattatta aattttcact agagcatggt taagcacata gtcgagtcct
     3301 ttatcttatc ctaagtgttt tccccaactt ttgtgtcatc catttccaaa tgaagagagc
     3361 tatgtaaaaa tctaccaatc catgtagcaa agagcctagt cctatcaaag aaaatagtga
     3421 ccataaataa gttgaactac tgttagaagt cattgtgatt atataaacag taataaattc
     3481 gagcagacca tgaattatgt taatagctac agcaaataac aatgtttgcc atttactcat
     3541 caataaagag ggctttgact taatcaagaa agccgcaatt aatgcaaatc cgatatgtga
     3601 taaagctcta agaacgatta caatcggaaa accagctagt agaaatccaa gtcctgtccc
     3661 taaagctacc aatattgcaa ctggaacaga tataaaaata cttaaaaata aaggtacatg
     3721 gcttgctaag gtgaaagaag caggcccaat aattatttta gcaggcatga ttataggaat
     3781 catgattgca aaagctacta aaatagaagt ataagaaacc atttgtgttg ttttttttga
     3841 catttttatc tcctttgaag ctattataca aaataacctt acaggtgtaa agacacctgt
     3901 aaggttattt gtctatgtat aaaattcctg ctttttttag agcctcacat atttggtcaa
     3961 aaactgactg atcttgacaa gaaatcatat gtgtatgtag accatctgtc aaagaggata
     4021 gcaattctgc attggaaact tttaatttag cagtaaaatt atcaatgtct ttttggcttt
     4081 gaatgtttaa gggagcagtc agcatcccat aaatagggtg ctctacctcc acattcataa
     4141 tgattccttg ataccgcaaa ataatttcca gttcttcttc tgtctgttct atgccatgct
     4201 gacaaaccaa tctagctgtg aattgatgag tacttagagc actggacata aggtatcctt
     4261 ttggggtaga aataatatca cattgttgtg cacgcaataa ggcaatatct ccaacaatca
     4321 cctgcctact aacactaaaa atctttgcta atgtgcttgc cgatatagct tcttttgttc
     4381 cttttaaagt cgttaaaata ttttctcgac gctcctgtgc cttcatataa caaattcctt
     4441 ctttggtgtt atttcacaat taaaaactgg ctagaatatc taaccagtta gcctcaatca
     4501 tttcgtgcaa aaagacgaag aatatttaag aatagattaa taaaatcaag ataaaggctt
     4561 agtgccatcg aaactgccca accatctgcg acttggccgc cttggctatt gtagacatat
     4621 ttaatcattt ggttatcgta agcaatcaat ccagaaaaga tgatgacaca tacgatactc
     4681 atgatataac tcataccccc actaccgata aataaattaa caagacttgc aattaaaatc
     4741 ccaattaaag cagccattaa cgctttacgc atacctgata aatctttttt agtcttagct
     4801 ccaatcaaag ccattgcaaa gaaaacagcc gcagatgtta taaaagcttg aagaactgta
     4861 gtttgcgtgt accttgcgat aatgaaactt aaagtaaacc cattcaaagc tgaataaatc
     4921 aaaaataatg gtaatgcagc tggtgtgttt cgacgcgccg ctccactagc aacaaagact
     4981 aaaaccaatt ctaagatgat tgctccaaaa tacaaccctg gcattttttg catgatggca
     5041 atcatatttt gtggaaacat atacagcata atcgcagaaa cagcagctga caaacctact
     5101 cctatcccta caagaccgta aatcttagca aaaaattgat tcagccctga atcagattgt
     5161 gtgtaaatga cgttatcatt catatgtatt ttcctattct aaattttaat gattatgact
     5221 acctaagaat ttgcgactaa aacggatagg tttcttaaaa atacgtttga gtggacgaga
     5281 agcctctcgt actgcccaat acttatcaac catagtcaca atataacttt ctttataacg
     5341 tggcggtgca atagtcgctc cccacatgtg cttcaaaata atatcttctt ctctagcgtt
     5401 taaatcagtt aattttcgcg cattacgaac cgctatcctt ggatgaaccc aggcatggct
     5461 cttgttaaat ttcgttacac gccaatcata ataaaagaaa tcatgtagta aaccacctcg
     5521 agcagtacta ctcgcatccc aaccaaactt cttagcaatt ttataacttg tataactaac
     5581 atttatagaa tgctctaagc gagttgagtg atgatgttgg acaatagctt cgagtttttg
     5641 gaaacgtgga tggtcaataa ggtgaccgac caaatccatg aagtccttat cgtcttgata
     5701 ctttttcatt ataatcaccc ctctggggat attataacat atgaacttaa attaagctga
     5761 aaatgactac tcccgcagct actgctacat taagactttc tgcttgtcca ggcatttcaa
     5821 tatgtgctaa aacatctgct tcttctgtca tagttttgga aattccctgt ccctcatttc
     5881 ccatgattaa agcaaaataa tcatatttag ggagattctt ataatttacc gattgttcag
     5941 ataaagttgt cgcaatcata gttatccctt ctgctttaca aaagttaaca aactgctcta
     6001 tttctaccct aaatactggt aaatgaaagt gactgccttg catagaacga agtgtttttt
     6061 ggttatacaa atcagcagat ttctgagata aaaaaacagc atcaaaattc gctgcatctg
     6121 cagttcgaac cattgttcct aggtttcctg gatcctgcac atcttctaat acaagaaaac
     6181 gtcctttttt aatatctgtc catcttgtct cttgaaaggc aatctcagct acaatccctt
     6241 gaggagtctg tgagtcagtt aattctttca aaacatcatc tgtaacaaca atagctctct
     6301 cgggttttct caaacgatct attgccgttt ctgtcacaaa aatatttaaa aattgtgcac
     6361 catatttttc agcttcctca aataaatgcc agccttcaat aagatatgaa gatttccgat
     6421 attttttttg taacaacttt ttcgtttttt taatgagatt atttgatttt gaagttataa
     6481 tagtagtcat aaacctatta tatcagaaaa gaggtcaagt tatgaaaaaa gttcatttaa
     6541 ttgtctcagg gcgcgttcaa ggagtcggat ttcgctatgc aacttattcc ttagctttag
     6601 aaataggtga tatttacggt cgcgtttgga ataacgatga tggaactgtt gaaattttag
     6661 cacaatccac agattcaaat aaaatgacac aatttattca gaaaattcga aaaggacctt
     6721 caaaatggtc taaagtaact tatgttgata ttaaactaga taacttcgat gattttaatg
     6781 actttaaaat gagtaattaa aaatcattct gcggaagtat tgtttaattc ttaaaaaaaa
     6841 agtaaaatag ataggtatat taaaaattag aggaactaaa aacattgaaa aaaacattga
     6901 aacgtatctt attctctagc ttatctttgt ctatgttatt attattgaca ggatgtgtta
     6961 gcgttgataa ggctggaaaa ccctatggag ttatctggaa tactcttgga gtgcctatgg
     7021 ccaacctcat tacttatttc gctcaacacc aagggcttgg ttttggtgtc gctatcatta
     7081 ttgtaaccgt catcgtacgt gtcgttatct taccacttgg actttatcag tcttggaaag
     7141 ctagctatca agctgaaaaa atggcgtact ttaagccttt atttgaacct attaatgaac
     7201 gactacgcaa tgctaaaaca caagaagaaa aattagcagc tcagacagaa ttaatgacag
     7261 ctcaacgtga gaatggcctt agcatgtttg gcggtattgg ctgcttgcca ttactcattc
     7321 agatgccatt tttctcagct attttctttg ctgcacgcta tactcctgga gtctctagtg
     7381 caaccttctt gggacttaac ttaggtcaaa agagtctgat acttactgtc attatcgcca
     7441 ttctttattt tgtacaatct tggttgtcaa tgcaaggtgt tccagatgaa caacgtcagc
     7501 aaatgaaaac aatgatgtat ctgatgccaa taatgatggt attcatgtct attagtttac
     7561 ctgctagtgt tgcgctttat tggtttattg gtggtatttt cagtattatc cagcagctag
     7621 ttacgactta tgtacttaag cctaaactac gccgaaaagt agaagaagaa tatactaaaa
     7681 atcctcctaa agcctacaaa gctaataacg cacgaaaaga tgttacaaat tcaactaaag
     7741 ccactgaatc taatcaagca attattacat ctaaaaaaac aaatcgtaac gccggaaaac
     7801 aaaaaagacg gggataaaac aagcatacta aataatggtg tgttgtccta aacagttaaa
     7861 gattattatg taattcaagt aaaaaagact tagttctatg tcctacatag ctaagtcttt
     7921 ttagttttgc ctaacttttc atataaaaag caccctaaaa gtcagataaa aatctagctt
     7981 ttaaagtgca agtaatctag ctaacagact accttattaa taaagtaatc gtttttccca
     8041 ccagtcttga ataattgcat aaagcgtaca aattacccaa tagataaggg ctactaagat
     8101 ataggcggtc atatagtcgg agtaggttcc cccaactatt ttagcattat taaagatatc
     8161 aggtaccgta atcatcgcag tcaatgagga acccttgacc atatctagca aaacattact
     8221 taaagaaggg agtgcaattc ttgttgcttg cggaataata atgcctctat aaatcgtcgg
     8281 tgtttttaac cctaaagctc tagcagcttc ccactgacca tgatcaacag ctaaaagagc
     8341 tgctctgata atttctgata tatatgcact agacatcatg gtaaaaccta cgatagaagc
     8401 agtcacagca tctaactgta tccctataaa aggcaaacca aagtagataa gaaagagtag
     8461 aaccattaaa ggaatcccac gcattaagga aatatgaata ttagctagat acctcagtaa
     8521 gcggaaatga gacatcttca ttagagctac aaagaatcct acgatagctc ctaaaataaa
     8581 gccaaccaag gataatccaa tggtatatgg taacccctga ataacacttg gaaaagcctt
     8641 aacagctagt tccaaattaa aaatggcatc ccagtttatc acttatctac tcctctcgaa
     8701 attttttatt taactttaat tactggaatt ttatccgttc cagcaggttc ttttgtgaga
     8761 tcttgaccag cataatattt ttcagagatt tttttcaaag aaccgtcttt cttcatatcc
     8821 ttgatagctg catcaatttt tgttttgagg ctcttatctt ttttactcat cacaatacct
     8881 tgctcagtcg gattatactt cacatcaccc atttttactt tgatatctgg atattgtttt
     8941 gttacatatt ttacagcaat gacttgtgtg tagtaatcat tcggaatgaa atctgtacgt
     9001 ccagttgata catctcgaag gtaaacgtca ttagttacat tgtcataaat aacaggttca
     9061 gcaccttgct gttttgcaat tttcatatat tgcgttccag cgccaccacc agctttttta
     9121 cctttccagt cagataaatc ttttgctgta atctttgaag aaccatctgc acgtacaatc
     9181 atacctccaa cagaatattt ataaggttga gagaaattgt attttttcaa acgctcggga
     9241 gttgtgtcaa agttattgac agcaacatcc accttaccac tatctacaga agtaaatgat
     9301 tcagcaactc caacttcaac aaacttaact tttatcttaa gttttttagc aatcgctttc
     9361 atcatatcaa tctcataacc agtcaatttt ttatggtcat cataataact tgttggataa
     9421 agcgttgcag gggtagcaac cttaaggaca ccatcttttt taatattgtc ccatgttgtt
     9481 tctttgttag attgaccatt tgagcaagct gctaatgtca ttgcagataa accgactaaa
     9541 ccaagtaaaa ttgtcttttt cataagtact cctgtagatt aaattatatt aaaatggttt
     9601 ggcgtactct atcgaaaatt aaatttaatg aaaattaata cataaattta cgataataat
     9661 acaaccattg ttaagagtac catattgtat ccgttttcgc aaaaatattg tttacttttc
     9721 aaattaatgg taaacaggcc ctttgaattg cttctctttt tcaaacaact ctgcaacagc
     9781 aagcaataaa tcttctctcc ctttagccgc tgtcaattgt acacctaaag gtagcccctc
     9841 tttagttaga tgagttggta tggctaaaga aggttgccct gtcaagtttg gctgatgtgt
     9901 aaaaggtgtc caggccattg agtcttcaaa cattttccaa attaaatcct gttgttcact
     9961 tactgagaat tctcccatat gcctcaattg tttctggagt gtctcgtcta aatcaaattg
    10021 tccatgaaaa ggagctggtt gattagttgc tgctgttaaa attaaatcat aattttcatg
    10081 aaacctcgcc atggtagctg caaattgatc ccatgaatct aagagtttac tgtaatcttt
    10141 agccaatacc ctttgtccac tctgatacat agcccaggtc attaattcca tatcgctaaa
    10201 ttccattgaa cgccctaaac tcttttcgat gtcatcaaac attgctgctg tttcaacgct
    10261 attcatcata caataagttt tcatagaatg aataccatct aaaggttgtt ctactaattc
    10321 taaatgattc cctttttgag acagaaaatc tgcggcttct ttaatagctg ctttagcctc
    10381 acttgatacc tttgttttta agggagaatc catcaatact gctattttta ggggtttact
    10441 aacgctaaac tcgaaaagac tctctttgct gagttttttc aggggaaatg ggctttctac
    10501 ttgatatgat tgtaaataat ataatagtcg ttttgtatct ctaactgact ttgttaaagc
    10561 aaaatgacta gaagcccctt gccaacctct ataggatgaa ggtccaactg gtatacgtcc
    10621 tcgactcggt tttaaaccaa tcaaaccatt aaaacttgct ggaattctga ttgaccctcc
    10681 tccatcacta gctcctgcaa ttggaaccat tcctgaacta acagctgcgg ctgcaccacc
    10741 actagagcct ccagcatttc ttgaatggtc aaatggaaga ttaacattcc cgtgaagttg
    10801 accatcacta atattcttaa aaccaaattc aggggtattt gttcttccta aaataataaa
    10861 ccctaatttt tcaaaggact gcactaaata gtccgtttgt ttagcatgat agtgtttaaa
    10921 taattgtgaa cctgaggttg agagttgtcc tttttgattc tgtcccaaat ctttcagaag
    10981 aataggaact cctgcaaaag gagcatttga ttcatttgcg tatttggctg cttgacgcgc
    11041 ctcattatat tgcttgctga caacagcatt aacgcttaca ttctgttcct cgatcttgta
    11101 gattgcttgc tctactaatt cttgcgacga aatcttatgc tgttttatcg cttgtaccat
    11161 agcggtagca tctttaaaaa ccatataaac acctcataaa atgtattttt attataagat
    11221 aacaaaaaat acttaaaaaa aatagaataa cgtctagact ctagatatta ttctatgatt
    11281 tatactatta atttgttttt tcaacactaa tgatttcaac ttgatagcta ccagcaggtg
    11341 attcaattgt agctaagtct cctgtttttt taccaattaa agcatgagca attgggcttt
    11401 cattcgaaat ttttcctgaa aaaatgtcag ctccagcagc accaacgata tggtaagtat
    11461 ctttatcatt tgtaccaact tcttggacaa ggacagtttt accaattgca acctcatctt
    11521 tagcaactgc atcactatca ataatttcag cataacgaat ttttgtttct aaaatttgaa
    11581 tttgaccctc aacaaatgct tgttcgtctt ttgctgcatc atattcgcta ttttctgata
    11641 agtcaccata agaacgagca atcttgatac gctctacaac ttctggtcta cgaactaatt
    11701 ttaactcttc aagttctttt tcaagttgat ctttttccac ttgtgtcatt gggtaagttt
    11761 tttctgccat tttatttctc ttttcttttc attttttcaa gtcaaagata taaacatgat
    11821 gactcgcacc atgtttataa gcttagttaa tttgactatt gatatatttt tcaacgttag
    11881 ccttgtgtgt ttcaaaatct ttagcgtagt atacatttcc ggtcttcaca tcagcgacaa
    11941 aataaaggta atctgttgaa gcaggtttta tagtcgcttc aattgctgag atacttggac
    12001 tatcaactgg tccaggcatc aaaccagtat tagtatagat gttgtatggt gatttaattg
    12061 aagtattaat ttgagcatct tcagctaaac ttgttttatc acctaatttc cccatagcat
    12121 acaaaattgc aatgttactt tgaagagctt gaccggctga taatcggtta taaaagacac
    12181 ttgctatttt acggcgatct tcgtctgttg atccctcttt ttcaacaaga gatgataatg
    12241 tcaaaacatc atttacagac atattcttcg ctttaatagt gttatagtaa ggtgctatct
    12301 tagtattcat tgttgaaatc atatcttcaa caagtccttc taaggttgt
//
LOCUS       DATROQ010000056        11717 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.057, whole genome shotgun sequence.
ACCESSION   DATROQ010000056 DATROQ010000000
VERSION     DATROQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11717
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.057"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            152..223
                     /locus_tag="VBC94_001376"
     tRNA            152..223
                     /locus_tag="VBC94_001376"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:184..186,aa:Gln,seq:ttg)
     gene            232..303
                     /locus_tag="VBC94_001377"
     tRNA            232..303
                     /locus_tag="VBC94_001377"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:265..267,aa:Glu,seq:ttc)
     gene            366..884
                     /gene="rimM"
                     /locus_tag="VBC94_001378"
     CDS             366..884
                     /gene="rimM"
                     /locus_tag="VBC94_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEO6979646.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVTDFVEERFKKGQVLALFDEK
                     NQFVMDIEIASHRKQKNFDIIKFKGMYHINDIEKYKGFTLKVAEDQLSDLKDGEFYYH
                     EIIGLDVYEGEELIGKIKEILQPGANDVWVVERHGKRDLLLPYIPPVVLEVNLSNQRV
                     QVELMEGLDDED"
     gene            871..1626
                     /gene="trmD"
                     /locus_tag="VBC94_001379"
     CDS             871..1626
                     /gene="trmD"
                     /locus_tag="VBC94_001379"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEO6979647.1"
                     /translation="MMKIDILTLFPEMFAPLEHSIVGKAKERGLLEINYHNFRENAEK
                     SRHVDDEPYGGGQGMLLRAQPIFDTIDKIDAQKARVILLDPAGRTFDQDFAEELSKED
                     ELIFICGHYEGYDERIKSLVTDEVSLGDFVLTGGELAAMTMVDATVRLIPEVIGKETS
                     HQDDSFSSGLLEYPQYTRPYDYLGMTVPDVLMSGHHENIRKWRLEQSLRKTLERRPDL
                     LENYAMTDEERLILEKIKTEIERTDTVNEQNNL"
     gene            1607..2599
                     /locus_tag="VBC94_001380"
     CDS             1607..2599
                     /locus_tag="VBC94_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6979648.1"
                     /translation="MNKTIYDITIVGGGPVGLFAAFYAGLRGVSVKIIESLSELGGQP
                     AILYPEKKIYDIPGYPVITGRELIDKHIEQLERFKDSIEICLKEEVLSFEKVDDVFTI
                     QTDKDQHLSRAIVFACGNGAFAPRLLGLENEENYADNNLFYNVTKLEQFAGKHVVICG
                     GGDSAVDWANELDKIAASVAIVHRRDAFRAHEHSVDILKASGVRILTPYVPIGLNGDS
                     QRVSSLVVQKVKGDEVIELPLDNLIVSFGFSTSNKNLRYWNLDYKRSSINVSSLFETT
                     QEGVYAIGDAANYPGKVELIATGYGEAPVAINQAINYIYPDRDNRVVHSTSLIK"
     gene            2736..3806
                     /locus_tag="VBC94_001381"
     CDS             2736..3806
                     /locus_tag="VBC94_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO6979649.1"
                     /translation="MTNTVTPKETAGSFINKVLGGTATAIVVALIPNAILATFLKPFL
                     SYGLAAEFLHIVQVFQFFTPIMAGFLIGQQFKFTPMQQLAVGGAAYIGSGAWAYTEVI
                     QKGVATGSFQLRGIGDLINMMLTAALAVLAVKWFGNKFGSLTIILLPIIIGTGVGYLG
                     WKLLPYVSYVTTLIGQGINSFTTLQPIAMSILIAMAFSMLIVSPISTVAIGLAIGLNG
                     MSASAASMGVASTTAVLVWATMKANKSGVPIAIALGAMKMMMPNFLKHPVMAIPMLMT
                     ATVSSLTVPLFKLVGTPASSGFGLVGAVGPIASFEAGASMLIVILSWLVIPFAVGFVS
                     HKICKDILKLYKDDIFVFEGQN"
     gene            3819..4742
                     /locus_tag="VBC94_001382"
     CDS             3819..4742
                     /locus_tag="VBC94_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEO6979650.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNHDVILLDNWADHIMAIKENGL
                     KVTGDTEDLVKLPIMKPTDATEEADLIILFTKAMQLPNMLQDIKKIIGKKTKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLKGPGHAHLEGVGSVNLQSIDPNNQEAGHRV
                     TELLNEAKLQATYDENVLPNIWRKACVNGTMNSTCALLDCTIGQLFASEDGVNMVHEI
                     IHEFVTVGKAEGVELDEEEITKYVMDTSVKAAHHYPSMHQDLVQNQRLTEIDFLNGAV
                     NKKGENLGIDTPYCRLITQLIHTKENVLSIK"
     gene            4954..6588
                     /locus_tag="VBC94_001383"
     CDS             4954..6588
                     /locus_tag="VBC94_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEO6979651.1"
                     /translation="MMKKGQVNDTKQSYSLRKYKFGLASVILGSFIMVTSPVFADQTT
                     SVQVNNQTGTSVDANNSSNETSASSVITSNNDSVQASDKVVNSQNTATKDITTPLVET
                     KPMVEKTLPEQGNYVYSKETEVKNTPSKSAPVAFYAKKGDKVFYDQVFNKDNVKWISY
                     KSFGGVRRYAAIESLDPSGGSETKAPTPVTNSGSNNQEKIATQGNYTFSHKVEVKNEA
                     KVASPTQFTLDKGDRIFYDQILTIEGNQWLSYKSFNGVRRFVLLGKASSVEKTEDKEK
                     VSPQPQARITKTGRLTISNETTTGFDILITNIKDDNGIAAVKVPVWTEQGGQDDIKWY
                     TAVTTGDGNYKVAVSFADHKNEKGLYNIHLYYQEASGTLVGVTGTKVTVAGTNSSQEP
                     IENGLPKTGVYNIIGSTEVKNEAKISSQTQFTLEKGDKINYDQVLTADGYQWISYKSY
                     SGVRRYIPVKKLTTSSEKAKDEATKPTSYPNLPKTGTYTFTKTVDVKSQPKVSSPVEF
                     NFQKGEKIHYDQVLVVDGHQWISYKSYSGIRRYIEI"
     gene            6732..7817
                     /locus_tag="VBC94_001384"
     CDS             6732..7817
                     /locus_tag="VBC94_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6979652.1"
                     /translation="MTLRELTIEEFKEHSGNYDSQSFLQTPEMAKLLEKRGYDVRYLG
                     YQVENKLEIISLSYIMPVTGGFQMKIDSGPVHSNSKYLKQFYKALQGYAKSNGVLELI
                     VEPFDDYQLFTSSGVPSNQGNDNLIEDFTSSGYHHDGLTTGFTGKYLSWHYVKNLEGV
                     TSETLLSSFSKTGRALVKKAMSFGIKVRVLKRDELHLFKEITTSTSNRRDYMDKSLDY
                     YQDFYDSFEGKAEFVIATLNFREYDHNLQIKAEALENKLKLLDERFRENADSPKYHRQ
                     RSEIINQLASFETRRQEVQSFIQKYDNQDVVLAGSLFVYSLKETVYFFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRGSTFYNLLGI"
     gene            7842..7967
                     /locus_tag="VBC94_001385"
     CDS             7842..7967
                     /locus_tag="VBC94_001385"
                     /inference="COORDINATES: protein motif:HMM:NF014445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEO6979653.1"
                     /translation="MLRFKQNFNGYIIRKMGTFNYYPSPFKYKGIQLLKKVLKRN"
     gene            8032..8841
                     /locus_tag="VBC94_001386"
     CDS             8032..8841
                     /locus_tag="VBC94_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6979654.1"
                     /translation="MLDIERKRDIIKLQTISIQGGTMLKSKRKEIILSRLEQNKSVTL
                     DELTSILETSESTVRRDLDELESEGFLKRVHGGAELPYSLGQELSNQEKSIKNVQKKL
                     DIARQTAKLIAKQDVIFIDAGTTTELLIDFLPHEQLTVVTNSIHHAAKLVDRGIKTII
                     IGGAVKHSTDASIGQVAINQIRQITVDKAFLGMNGIDEVYLTTPDLEEAAIKEAIINN
                     SQQTFILMDSSKIGQVTFAKVKEINDINLVTNKTDSELMTIIKEKMKVIQV"
     gene            8838..9749
                     /gene="pfkB"
                     /locus_tag="VBC94_001387"
     CDS             8838..9749
                     /gene="pfkB"
                     /locus_tag="VBC94_001387"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEO6979655.1"
                     /translation="MIYTVTLNPSIDFIVRLDTLLLGSVNRMTSDDKYVGGKGINVSR
                     ILKRLKIDNTATGFIGGFTGHFVEDGLVSEGIKTDFVSVNEDTRINVKVKAKIETEIN
                     GGGPRITNEQLHRLEKLLSRLTPEDTVVFAGSAPASLGNKVYNTLIPIAKKTGAEVVC
                     DFEGQTLLDALAYQPLLVKPNNHELADIFGVELEGLPDIEKYAHKILDKGAKNVIVSM
                     AGDGALLVTPEASYFAKPIKGEVKNSVGAGDSMVAGFTGEFVKSKNPVEALKWGVACG
                     TATTFSDDLATAEFIQDIYNKVEVEKL"
     gene            9746..11710
                     /locus_tag="VBC94_001388"
     CDS             9746..11710
                     /locus_tag="VBC94_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6979656.1"
                     /translation="MKIQDLLKKEVMIMDLKATSKEAAIDEMITKLVDTGVVTNFAIF
                     KDGIMKREAQTSTGLGDGIAMPHSKNAAVKEATVLFAKSASGVDYEALDGQPTDLFFM
                     IAAPDGANDTHLAALAELSKYLLKEGFADQLRQAKTPDDIIATFDSNSISQETVAPQT
                     VQSTSKGSDYIVAVTACTTGIAHTYMAEEALKKKAAEMGVGIKVETNGASGVGNKLTS
                     SDIARAKGVIIAADKAVEMDRFDGKPLVSRPVADGIKKSEDLINIILDNKAQTYHAKN
                     QNDKQSGESDGKSGLGSAFYKHLMGGVSQMLPFVIGGGIMIAIAFLFDNILGVPKDQL
                     SNLGSYHEIAALFKNIGGAAFAFMLPVLAGYIAYSIAEKPGLVAGFVAGSIASSGLAF
                     GKVPFAEGGKATLALAGVPSGFLGALVGGFLAGGVILLLRKLLSGLPKSLEGIKSILL
                     YPLLGVLITGFLMLLVNIPMAAINTALNTFLQGLSGSSAVLMGLLVGGMMAVDMGGPV
                     NKAAYVFGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFNNEERQSGL
                     TNIVMGLSFITEGAIPFGAADPARAIPSFIVGSALTGALVGLAGIKLMAPHGGIFVIA
                     LTSNPLLYILFILIGAIVSGVLFGLFRKTK"
BASE COUNT         3901 a         1748 c         2399 g         3669 t
ORIGIN      
        1 aagtttttga atatatttca aaaatgaaac ataaaatact ttgatagact ctatatgagc
       61 tttattaaaa gggataaaat cgaaatttaa aatttaattc gaaaagatat tgacagtaat
      121 ctagaatttc gatatactag attttgtctt ttggggtata gccaagcggt aaggcaaggg
      181 actttgactc cctcatgcgt tggttcgaat ccagctaccc cagtttcaca tggtccgttg
      241 gtcaaggggt taagacaccg ccttttcacg gcggtaacac gggttcgaat cccgtacgga
      301 ctatcgtggc cagttgcatt ttagcggctg gtttttcttt tattttcaaa aatatcggag
      361 ttactatgga atactttaac gttggaaaaa ttgtcaatac acaaggatta caaggcgaaa
      421 tgcgtgtatt aagcgtgact gactttgtag aagaacgctt taaaaaaggt caagtgttag
      481 ctttattcga tgaaaagaat cagtttgtta tggatattga aattgctagt catcgtaagc
      541 agaagaattt tgacattata aaattcaaag gaatgtatca tattaatgat attgaaaaat
      601 ataaaggttt cacactgaag gtggcagagg atcaactgag cgaccttaaa gatggtgaat
      661 tttattatca tgaaattatt ggcttagatg tctatgaagg ggaagagctg attggaaaaa
      721 tcaaagaaat tttacaacca ggagcaaatg acgtttgggt tgtggaacgt catggaaaac
      781 gtgatctttt attaccctat atcccgccag tagtattaga agtgaacctt tctaatcagc
      841 gtgttcaagt tgaattgatg gaaggtttag atgatgaaga ttgatatttt aactttattt
      901 cctgaaatgt ttgcccctct ggaacattct atagttggta aggcaaaaga aagaggactt
      961 cttgaaatta attatcacaa tttcagagaa aatgctgaga agtctcgaca tgttgatgat
     1021 gagccttacg gtggggggca agggatgctt ttacgtgctc aacctatttt tgatacaatt
     1081 gataaaatag atgctcaaaa agcacgtgtt attttgctag acccagcggg cagaacattt
     1141 gatcaggatt ttgcagaaga actatccaaa gaagatgaat taatttttat ttgtggtcac
     1201 tatgaaggtt atgatgaacg tataaaatca ttagtaacag acgaagtatc tttgggagat
     1261 tttgttctta cgggaggaga attggcggct atgacaatgg tcgatgctac cgttcgtctg
     1321 attccagaag ttataggtaa agaaacaagt catcaggatg attcattttc atcgggccta
     1381 ttagaatatc ctcagtatac gcgcccatat gattatttag gcatgacagt tcctgatgtt
     1441 ctcatgagtg gtcatcatga aaatatccga aaatggcgtt tagaacaaag tttgcgaaaa
     1501 acactagaac gtcgaccaga cttgttagaa aattatgcaa tgacggatga agagagactt
     1561 atcttagaga aaattaaaac agaaatagaa agaacagata cagtaaatga acaaaacaat
     1621 ttatgatatc actattgtag gaggcggacc agtaggtctc tttgctgctt tttatgctgg
     1681 actacgcgga gtatccgtta aaattattga aagcctttcg gaattaggag gccagccagc
     1741 tatcctttat cctgagaaaa aaatctatga tattcctggt tatccggtga ttactggacg
     1801 tgaattaatt gataaacata ttgaacaatt agagagattt aaagactcta ttgaaatctg
     1861 tttaaaagaa gaagttttat catttgaaaa agtagatgat gttttcacaa ttcaaacaga
     1921 taaagatcaa catttgtcac gtgctattgt ttttgcttgt ggcaacggtg cttttgctcc
     1981 gcgtttacta ggtttggaaa atgaagaaaa ttatgcagat aataatttat tctataacgt
     2041 tacaaagtta gaacagtttg caggtaaaca tgttgttatt tgtggtggag gcgactcagc
     2101 tgttgattgg gccaatgaat tagataagat tgcagctagt gtagcgattg ttcatcgtag
     2161 agatgctttt agagcgcatg agcatagtgt ggatatacta aaggcatcag gtgttagaat
     2221 tttaacccct tatgtcccaa ttggtttaaa tggagatagt cagagagtta gcagcttggt
     2281 cgttcaaaaa gttaaaggtg atgaagtaat tgaattacct ttagacaact tgattgttag
     2341 ttttggcttt tctacttcta ataaaaattt acgttattgg aatttggatt ataaacggtc
     2401 tagtatcaat gtttcttcac tttttgagac gactcaagaa ggcgtgtatg ctattggaga
     2461 tgctgctaat tatccaggaa aggtagaatt gattgcaaca ggatatgggg aagcaccagt
     2521 cgctataaac caagctatca attatattta tcctgatcga gataaccgtg tagtccattc
     2581 aacatcattg ataaaataac tataaataaa actgataagt ttataaaaaa ggattatata
     2641 tattgttaaa aaaataacaa aacttcctat ttattttgtg atataattaa ctttgtcgtt
     2701 atgaatgata ttaaataaat gaagcgaggt attttatgac aaatacggta acaccaaaag
     2761 agacggctgg ctcttttatc aataaagtgt taggtgggac agcaactgca atcgttgtag
     2821 cactcatccc aaatgctatc ctagccactt ttttgaaacc atttttatct tacggacttg
     2881 ctgcagagtt cttacacatc gtgcaggtat ttcaattttt cacaccaatt atggcaggat
     2941 ttttgattgg tcaacagttt aaattcaccc caatgcaaca gcttgctgtt ggaggtgctg
     3001 catatattgg ttctggtgcg tgggcttaca cagaagttat tcaaaaagga gttgctactg
     3061 gtagtttcca attgcgtggg attggtgatc tcatcaatat gatgctaaca gcagctttag
     3121 cagttttagc agttaagtgg tttggtaata aatttggttc cctaaccatt atcttgttac
     3181 ctattatcat aggtacaggc gtaggctatt taggatggaa attactccct tacgtttcat
     3241 atgtgacaac acttattggt caaggaatta attcttttac tactttacaa ccaattgcga
     3301 tgtcaattct aattgcgatg gctttttcaa tgcttattgt aagtcctatt tcaacagtag
     3361 cgattggtct agcgattggt cttaatggta tgtcagcaag tgcagcttca atgggagttg
     3421 cttcaacaac agcagtgcta gtttgggcaa cgatgaaggc taataagtct ggagttccaa
     3481 ttgctattgc tttaggtgca atgaagatga tgatgccaaa tttcctaaag catccagtaa
     3541 tggcgattcc aatgcttatg actgctacag taagttctct aacagtccca ttgtttaaat
     3601 tggtggggac accagcttcg tctggttttg ggcttgttgg tgcagtaggt ccgattgctt
     3661 catttgaagc aggagcaagt atgttgattg ttatcttatc ttggctcgtt attccttttg
     3721 cagtaggttt tgtatcacac aaaatttgta aagacattct gaaactttat aaagacgata
     3781 ttttcgtttt tgaaggtcaa aattaatttg gaggttgtat gttagtttat attgctggtt
     3841 caggcgctat gggatgtcgt tttggatatc aaatttctaa aacaaatcat gatgttattt
     3901 tacttgataa ttgggcagac catattatgg ctattaaaga aaatggcttg aaggtaacag
     3961 gtgatacaga ggaccttgtt aaacttccta tcatgaagcc aactgatgcg acagaagaag
     4021 cagatttaat tattttgttt actaaagcaa tgcagttgcc aaatatgctt caagatatta
     4081 aaaaaattat tggtaagaaa acaaaggtct tatgtctttt aaatggatta ggacatgaag
     4141 atgtcattcg tcaatatatc cctgaacaca atattttgat gggagtaaca gtttggactg
     4201 cagggttgaa aggaccaggg catgctcatc tggaaggagt tggttctgta aatcttcaaa
     4261 gtatcgatcc taacaatcaa gaagcaggtc atcgcgttac tgaattgctt aatgaagcaa
     4321 aattacaggc tacttatgac gaaaatgttt tgccaaatat ttggagaaaa gcttgtgtta
     4381 acggaacgat gaattcaact tgcgccttgc tagattgtac aattggtcaa ctctttgcta
     4441 gtgaagacgg cgtaaatatg gtacatgaaa ttatccatga atttgttact gttggaaaag
     4501 cagaaggtgt agagcttgat gaagaagaaa taacaaagta cgttatggat acatctgtta
     4561 aagcagccca tcattaccct tcaatgcatc aagacttagt tcaaaatcag cgcttaactg
     4621 aaattgattt cttaaatggc gcagtgaata aaaaaggtga aaacctaggc attgatacac
     4681 cttactgtcg attaatcaca caattaattc acactaaaga aaatgtactt agcataaaat
     4741 aataatcaac cgggagaata gtgcccggtt ttttgatatg cttaatgata atgattttgt
     4801 tatactaaga ataataagag ttgggtattt ttttcgagaa gtatttacag tataaaatat
     4861 tacatttgta ttacaaatat ttctaaaata tgttgacatg ttacaaaaag gatattataa
     4921 tagtagtgta aataaataag gaagtaggtg tttatgatga aaaaaggaca agtaaatgat
     4981 actaagcaat cttactctct acgtaaatat aaatttggtt tagcatcagt aattttaggg
     5041 tcattcataa tggtcacaag tcctgttttt gcggatcaaa ctacatcggt tcaagttaat
     5101 aatcagacag gcactagtgt ggatgctaat aattcttcca atgagacaag tgcgtcaagt
     5161 gtgattactt ccaataatga tagtgttcaa gcgtctgata aagttgtaaa tagtcaaaat
     5221 acggcaacaa aggacattac tactccttta gtagagacaa agccaatggt ggaaaaaaca
     5281 ttacctgaac aagggaatta tgtttatagc aaagaaaccg aggtgaaaaa tacaccttca
     5341 aaatcagccc cagtagcttt ctatgcaaag aaaggtgata aagttttcta tgaccaagta
     5401 tttaataaag ataatgtgaa atggatttca tataagtctt ttggtggcgt acgtcgatac
     5461 gcagctattg agtcactaga tccatcagga ggttcagaga ctaaagcacc tactcctgta
     5521 acaaattcag gaagcaataa tcaagagaaa atagcaacgc aaggaaatta tacattttca
     5581 cataaagtag aagtaaaaaa tgaagctaag gtagcgagtc caactcaatt tacattggac
     5641 aaaggagaca gaatttttta cgaccaaata ctaactattg aaggaaatca gtggttatct
     5701 tataaatcat tcaatggtgt tcgtcgtttt gttttgctag gtaaagcatc ttcagtagaa
     5761 aaaactgaag ataaagaaaa agtgtctcct caaccacaag cccgtattac taaaactggt
     5821 agactgacta tttctaacga aacaactaca ggttttgata ttttaattac gaatattaaa
     5881 gatgataacg gtatcgctgc tgttaaggta ccggtttgga ctgaacaagg agggcaagat
     5941 gatattaaat ggtatacagc tgtaactact ggggatggca actacaaagt agctgtatca
     6001 tttgctgacc ataagaatga gaagggtctt tataatattc atttatacta ccaagaagct
     6061 agtgggacac ttgtaggtgt aacaggaact aaagtgacag tagctggaac taattcttct
     6121 caagaaccta ttgaaaatgg tttaccaaag actggtgttt ataatattat cggaagtact
     6181 gaagtaaaaa atgaagctaa aatatcaagt cagacccaat ttactttaga aaaaggtgac
     6241 aaaataaatt atgatcaagt attgacagca gatggttacc agtggatttc ttacaaatct
     6301 tatagtggtg ttcgtcgcta tattcctgtg aaaaagctaa ctacaagtag tgaaaaagcg
     6361 aaagatgagg cgactaaacc gactagttat cccaacttac ctaaaacagg tacctataca
     6421 tttactaaaa ctgtagatgt gaaaagtcaa cctaaagtat caagtccagt ggaatttaat
     6481 tttcaaaagg gtgaaaaaat acattatgat caagtgttag tagtagatgg tcatcagtgg
     6541 atttcataca agagttattc cggtattcgt cgctatattg aaatttaaag ctaaatcttt
     6601 ttagatttag ctttactaat attatgtgtg ttggaaaaaa cagtggagat ttacgtcaaa
     6661 attatattat ttggatagta tcgaatagat agaatgtgga gaaaagtagg aaatagttaa
     6721 gaaaggtatt tatgacgctt cgagaattaa caatagaaga atttaaagaa cattcaggaa
     6781 attatgattc acaatcattt ttacaaacac ctgagatggc taaactttta gaaaaacgcg
     6841 gctatgatgt taggtatttg ggatatcaag tagaaaataa actagagata atcagtttat
     6901 cttatattat gccagtcact ggtggttttc aaatgaaaat tgattcagga ccagttcatt
     6961 caaattctaa gtatctaaaa caattttata aagcattgca aggctatgcc aaatccaacg
     7021 gtgttctaga attaatagtt gagccttttg atgattacca attattcact agttcgggag
     7081 ttcctagtaa tcagggaaat gataatctga ttgaagattt taccagttca ggttatcacc
     7141 atgatggttt aacaactggt tttactggta aatatttatc ttggcactat gttaaaaatt
     7201 tagaaggtgt cacttctgaa acgttactat cttcattctc taagacagga cgagctttgg
     7261 ttaagaaagc aatgtctttt ggaatcaagg ttcgcgttct taaacgtgat gagctacatt
     7321 tatttaaaga gataacaact tctacgtcaa atagacgtga ttatatggat aagtccttag
     7381 attattatca agatttttac gatagctttg aaggcaaggc tgaatttgtg attgccactt
     7441 taaattttag agaatacgac cataacttgc aaataaaagc tgaagcattg gaaaataagc
     7501 ttaaattgct tgatgaacgt tttagggaaa atgcagactc tcctaagtat cataggcaac
     7561 gctctgaaat aattaatcaa ctggctagtt ttgaaacacg acgacaagaa gttcagtcat
     7621 ttattcaaaa gtatgataac caagacgttg ttttagctgg tagtctattt gtttatagtc
     7681 ttaaggagac agtttatttc ttttcaggat cctacacaga atttaataaa ttttatgcac
     7741 cagcagttct tcaggaatat gttatgcaag aggctctcaa aagaggttct actttctata
     7801 acctcctagg tatttaggga acttttgatg gttcagatag tattcttcgc tttaaacaga
     7861 attttaatgg ttatatcatt cgtaaaatgg gaacttttaa ctattatcca agtcctttta
     7921 aatacaaagg aattcagctt cttaaaaagg ttttgaagag aaattaaagt atttgtaaaa
     7981 gctaatcttt agattaggct tttttagttt attgatagtt tttggaagaa aatgcttgac
     8041 attgaaagaa aaagagatat aattaaattg caaacgattt caattcaagg agggactatg
     8101 ctaaagtcaa agagaaaaga aattatctta tcacgacttg agcaaaataa gagtgttact
     8161 ttagatgaat taacgtctat cttagagact tctgaatcta cagttcgcag ggatttagat
     8221 gaacttgaat ctgaaggttt tttaaaaagg gtgcatggtg gtgctgaatt accatactca
     8281 cttggccagg aattaagtaa tcaagaaaaa tctatcaaaa acgttcaaaa aaaattggat
     8341 attgctagac aaactgcaaa gctgatagca aagcaagatg ttatatttat tgatgccgga
     8401 actacgacag aattattaat tgattttctc cctcacgagc aattaacggt ggtgacgaat
     8461 tctattcatc atgctgcaaa gttagttgat agagggataa aaacaatcat tatcggagga
     8521 gcggttaaac attcaacaga tgctagtatt ggccaggtag ccattaatca gattaggcag
     8581 ataacggttg acaaagcttt tctggggatg aatggtatag atgaagttta tttaactaca
     8641 ccagatttag aagaagctgc tattaaagaa gctattatta ataatagtca acaaaccttt
     8701 attctaatgg attcatctaa aattggacaa gttacatttg ctaaagtgaa agagataaat
     8761 gacattaatt tagtaactaa taagactgat tcagaactta tgacaataat aaaagagaaa
     8821 atgaaggtga ttcaagtatg atttatacag taacattaaa tccatcgatt gattttattg
     8881 ttcgtctaga tacactattg ttgggtagtg ttaaccgtat gactagcgat gataaatatg
     8941 taggtggtaa aggaattaat gttagtcgca tattaaaacg cttaaaaatt gacaatactg
     9001 ctacaggttt tatcggtgga tttacaggac attttgttga agatggactt gtatcagaag
     9061 gtatcaaaac tgactttgtt tctgttaatg aagatacacg tatcaacgtt aaagtaaaag
     9121 caaaaattga aactgaaatc aatggcgggg gtcctaggat aacaaatgaa caacttcatc
     9181 gtctagagaa acttctttcg agactaacac ctgaggacac agtagttttc gcaggttcag
     9241 caccagctag ccttggaaat aaggtctata atacattgat tccaattgct aaaaagacag
     9301 gagcggaagt tgtttgcgat tttgaaggtc agacactttt agacgctctt gcttatcagc
     9361 ctcttttagt aaaaccgaat aaccatgaat tagcagatat ttttggcgta gagctagaag
     9421 gtctgcctga tatcgaaaaa tatgcacata agattctaga taaaggagct aagaatgtta
     9481 tcgtttctat ggcaggggat ggtgcattgt tagtaactcc agaagcttca tattttgcaa
     9541 aaccgattaa gggtgaagtg aaaaattccg ttggggctgg tgattcaatg gtagctggct
     9601 ttacaggaga atttgttaag tcaaaaaatc cagtagaagc actaaaatgg ggcgtagctt
     9661 gtggtacagc aaccactttc tcagatgatt tagcaacagc tgagtttatc caagatattt
     9721 ataataaagt tgaggtagaa aaattatgaa aatccaagac ctactaaaaa aagaagttat
     9781 gattatggat ttaaaagcaa ccagtaaaga ggctgcaatt gatgaaatga ttacaaaatt
     9841 agttgacaca ggtgttgtga caaactttgc tatttttaaa gatggtatca tgaagcgtga
     9901 ggcacaaaca tcaactggat taggtgatgg tatcgctatg ccacatagta aaaatgctgc
     9961 cgttaaagaa gctactgtcc tttttgcaaa atctgcatca ggtgttgatt acgaagcact
    10021 tgatggacag ccaactgatt tattctttat gattgctgcg ccagatggtg ctaatgatac
    10081 gcatttagct gccttggcag aactatctaa atatctttta aaagaagggt tcgctgatca
    10141 attacgtcaa gcaaaaacgc cagatgacat tattgctact tttgatagta atagcatttc
    10201 tcaagaaaca gtagctccac aaacagtaca atcaacaagt aaaggttcag attatattgt
    10261 tgcagtaaca gcttgcacta cgggaattgc ccacacgtat atggcagaag aagctttaaa
    10321 gaaaaaagca gctgaaatgg gtgtaggtat taaagttgaa acaaatggcg cttctggtgt
    10381 aggaaataag ttgacttcaa gtgatattgc aagggctaag ggtgttatta ttgcagcaga
    10441 taaggctgta gagatggatc gttttgatgg caaacctttg gtatcacgtc cggtagctga
    10501 tggtattaaa aaaagtgaag acttaattaa cattattctc gataataagg cacaaaccta
    10561 tcatgcaaaa aatcaaaatg ataaacaatc aggtgaatct gatggaaaat caggcttagg
    10621 ctctgctttt tataaacacc ttatgggtgg tgtatcacaa atgttaccat tcgttattgg
    10681 tggtggtatt atgattgcga ttgctttcct ttttgataac atacttggtg taccaaaaga
    10741 ccaattaagt aatttaggtt cataccatga aattgcagcc ttattcaaaa atatcggtgg
    10801 cgcagcattt gcatttatgt taccagtatt ggcaggttat atcgcttatt caatagctga
    10861 aaaaccagga ctagtagctg gcttcgtagc tggttcaatt gcatcaagtg gactggcatt
    10921 cggtaaagta ccttttgcag aaggtgggaa agcaacctta gcattagcag gtgtgccatc
    10981 aggtttctta ggggctttag taggtggttt cttagctggt ggagttatcc tactacttag
    11041 aaaattactt tcaggtctcc caaaatcgct tgaaggtatt aaatcaattc ttctataccc
    11101 attgttaggt gttttgatta caggtttctt gatgttgctc gtaaacattc caatggcagc
    11161 tatcaacaca gctcttaaca ctttcttaca aggtttatct ggtagttcag cagtcttgat
    11221 gggcttacta gttggtggta tgatggctgt agatatgggt gggcctgtta ataaagcagc
    11281 ttatgttttt ggtacaggta ctcttgcagc aactgttgca aatggcggct ctgttgttat
    11341 ggcagctgta atggctggag gaatggttcc accgttagca gtatttgtgg caactttatt
    11401 atttaaagat aaatttaata atgaagaacg tcaatctggt ctaacaaata tcgttatggg
    11461 gttatctttt atcactgaag gtgctattcc gtttggtgct gctgacccag ctcgtgcaat
    11521 ccctagcttt attgtaggtt cagccttaac tggagcactt gttggtttag ctggtatcaa
    11581 gttgatggct cctcatggag ggatttttgt catcgcatta acaagtaatc ctttacttta
    11641 tattctattt attctaattg gagcgatagt ctcaggtgta ctctttggct tgttccgtaa
    11701 aacaaaatag ttaatct
//
LOCUS       DATROQ010000057        11312 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.058, whole genome shotgun sequence.
ACCESSION   DATROQ010000057 DATROQ010000000
VERSION     DATROQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11312
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.058"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            29..832
                     /locus_tag="VBC94_001389"
     CDS             29..832
                     /locus_tag="VBC94_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO6979657.1"
                     /translation="MKKIIYLGLACVSILTLSGCESIERSLKGDRYVDQKLAENSSKE
                     ATEQLNKKTKQALKADKKAFPQLDKAVAKNEAQVLIKTSKGDINIKLFPKYAPLAVEN
                     FLTHAKEGYYNGLSFHRVIKDFMIQSGDPNGDGTGGKSIWNSKDKKKDSGNGFVNEIS
                     PYLYNIRGSLAMANAGADTNGSQFFINQSQQDHSKQLSDKKVPKVIIKAYSEGGNPSL
                     DGGYTVFGQVISGMETVDKIASVEVTKSDQPKEKITITSIKVIKDYKFK"
     gene            complement(884..1717)
                     /locus_tag="VBC94_001390"
     CDS             complement(884..1717)
                     /locus_tag="VBC94_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO6979658.1"
                     /translation="MFTKFFEGLLTYHFLQNAFITAIVIGIVAGAVGCFIILRSMSLM
                     GDAISHAVLPGVAISFILGINFFIGAIVFGLLSSIIITYIKENSVIKGDTAIGITFSS
                     FLALGIILIGLANSTTDLFHILFGNILAVQDSDKYMTIIVGLIVLTLITIFFKELLLT
                     SFDPVLAKSMGMRVSFYHYLLMILLTLVAVTAMQSVGTILIVALLITPAATAYLYVKS
                     LRTMLFLSSALGAVASVLGLYIGYTFNIAAGSSIVLTSTFMFLLAFLFSPKQSLFKKK
                     Q"
     gene            complement(1719..2435)
                     /locus_tag="VBC94_001391"
     CDS             complement(1719..2435)
                     /locus_tag="VBC94_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO6979659.1"
                     /translation="MIISKHLSVSYDNNLVLEDINLRLEGSGIIGILGPNGAGKSTLM
                     KALLGLVDSTGESGIGGDLLPLMGRVAYVEQKTNIDYQFPITVGECVSLGLYKERGLF
                     KRLSKTDWEKVSRVIDQVGLRGFENRPINALSGGQFQRMLMARCLVQEADYIFLDEPF
                     VGIDSISEQIIVNLLKKLSKAGKLILVVHHDLSKVDHYFDQVIILNRHLIACGPIDQA
                     FTRENLSAAYGDAILLGQGD"
     gene            complement(2606..3532)
                     /locus_tag="VBC94_001392"
     CDS             complement(2606..3532)
                     /locus_tag="VBC94_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEO6979660.1"
                     /translation="MKKWLVIVSCFVAFLGLGACANKQDQAKNEKLKVVVTNSILADI
                     TKNIAGNKIDLHSIVPVGKDPHEYEPLPADVEKTTQADLVFYNGINLETGGNAWFTKL
                     IKNAKKKENKDYFAVSDGVDVIYLNGQSGKGKEDPHAWLNLENGIIYSKNIAKQLIAK
                     DPKNKATYEKNRDAYVAKLEKLDKEAKSKFNAIPANKKLIVTSEGCFKYFSKAYGVPS
                     AYIWEINTEEEGTPDQITSLVKKLKQVRPSALFVESSVDKRPMKSVSRESGIPIYAEI
                     FTDSIAKKGQKGDSYYAMMKWNLDKIAEGLAK"
     gene            3699..4346
                     /locus_tag="VBC94_001393"
     CDS             3699..4346
                     /locus_tag="VBC94_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEO6979661.1"
                     /translation="MTPNKEDYLKCIHELGEKNNKISNKKIAEMMQVSAPAVSEMIKK
                     MISEQLIVKDKDLGYYLTKQGLLVVSDLYRKHRLVEVFLVNHLHYTADDIHEEAEVLE
                     HTVSTTFVDQLEKLLDFPQFCPHGGTIPKKGEFLVEINQMTLDQISQLGTYVISRVHD
                     DFQLLKYLEQHRLHINDTIELTQIDPYAKTYHITYNDENLTIPERIASQIYVSAT"
     gene            complement(4386..5075)
                     /locus_tag="VBC94_001394"
     CDS             complement(4386..5075)
                     /locus_tag="VBC94_001394"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEO6979662.1"
                     /translation="MKIGIIAAMEEELKLLVENLEDKSQETVLSNVYYSGRYGEHELV
                     LVQSGVGKVMSAMSVAILVESFKVDAIINTGSAGAVATGLNVGDVVVADTLVYHDVDL
                     TAFGYDYGQMSMQPLYFHSDKTFVSTFEAVLSKEEMTSKVGLIATGDSFIAGQEKIDV
                     IKGHFPQVLAVEMEGAAIAQAAQATGKPFVVVRAMSDTAAHDANITFDEFIIEAGKRS
                     AQVLMAFLKAL"
     gene            complement(5085..5354)
                     /locus_tag="VBC94_001395"
     CDS             complement(5085..5354)
                     /locus_tag="VBC94_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979663.1"
                     /translation="MGKPLLTDDMIERSNRGEKVSGQTILDQETKIISTEDGMEQLTD
                     ENGKHIYKSRRIENAKRNEFQRKLNLVLFILLILLALLFYAIFKL"
     gene            complement(5354..5908)
                     /locus_tag="VBC94_001396"
                     /pseudo
     CDS             complement(5354..5908)
                     /locus_tag="VBC94_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632172.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            complement(5929..7308)
                     /gene="glmU"
                     /locus_tag="VBC94_001397"
     CDS             complement(5929..7308)
                     /gene="glmU"
                     /locus_tag="VBC94_001397"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEO6979664.1"
                     /translation="MSNYAIILAAGKGTRMKSDLPKVMHKVSGITMLEHVFRSVQAIE
                     PSKIVTVIGHKAELVRDVLGDKSEFVMQTEQLGTGHAVMMAEEELATSKGHTLVIAGD
                     TPLITGESLKNLIDFHVNHKNVATILTADAANPFGYGRIIRNSDDEVTKIVEQKDAND
                     FEQQVKEINTGTYVFDNQSLFEALKDINTNNAQGEYYLTDVIGIFKEAGKKVGAYKLR
                     DFDESLGVNDRVALATAEKVMRHRIARQHMVNGVTVVNPDSAYIDIDVEIGEESVIEP
                     NVTLKGQTKIGKGTLLTNGSYLVDAQVGNDVTITNSMVEESIISDGVTVGPYAHIRPG
                     TSLAKGVHIGNFVEVKGSQIGENTKAGHLTYIGNAEVGCDVNFGAGTITVNYDGQNKF
                     KTEIGSNVFIGSNSTLIAPLEIGDNALTAAGSTITDNVPIDSIAIGRGRQVNKEGYAN
                     KKPHHPSQK"
     gene            complement(7552..7965)
                     /locus_tag="VBC94_001398"
     CDS             complement(7552..7965)
                     /locus_tag="VBC94_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6979665.1"
                     /translation="MVKALETYIVTNGNGRQAVDFYKDVFQADLVNMMTWEEMDPNCL
                     EDRKDLIINAQLIFDGIRLQISDENPDFVYQAGKNVTAAIIVGSVEEAREIYEKLKKS
                     AQEVQLELQETFWNPAYANLVDQFGVMWQISTELN"
     gene            complement(7978..8355)
                     /locus_tag="VBC94_001399"
                     /pseudo
     CDS             complement(7978..8355)
                     /locus_tag="VBC94_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625774.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(8444..9400)
                     /locus_tag="VBC94_001400"
     CDS             complement(8444..9400)
                     /locus_tag="VBC94_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6979666.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIEGIDLVAILSTVRSLETAKDLA
                     KEYNMSLATSEYKAVLDNEEIDTVYIGLPNHLHFDYAKEALLAGKHVICEKPFTLEAS
                     QLEELVSIANTRQLILLEAITNQYLPNFDLVKEHLSNLGDIKIVECNYSQYSSRYDAF
                     KRGEIAPAFNPEMGGGALRDLNIYNLHLVIGLFGEPITAQYLPNIERGIDTSGVLVLD
                     YGHFKTVCIGAKDCSAEVKSTIQGDKGSIAILGPTNTMPKISLTMNGQESHVYQLNGD
                     RHRMHDEFVIFEGIISNLDFKRAAQALEHSRTVMKVLDMVTK"
     gene            complement(9397..9849)
                     /locus_tag="VBC94_001401"
     CDS             complement(9397..9849)
                     /locus_tag="VBC94_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEO6979667.1"
                     /translation="MKAQELWNKFKSLNPSIGDEIDAWQFGAEPDLLAKLVVDGTKTA
                     TASAYDLYELDEEGLPQVGSYDIILDSNDEAICIIEITKVDIVPFKDVSADHAFKEGE
                     GDKTLEWWRKAHIDFFKPYFEEFGLMFSEDSRIVLEEFQVVYPKEINE"
     gene            complement(9846..10091)
                     /locus_tag="VBC94_001402"
                     /pseudo
     CDS             complement(9846..10091)
                     /locus_tag="VBC94_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233822.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3977 family protein"
     gene            complement(10088..10786)
                     /locus_tag="VBC94_001403"
     CDS             complement(10088..10786)
                     /locus_tag="VBC94_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEO6979668.1"
                     /translation="MTKVVLVTGCASGIGYAQAQYFLKQGYQVYGVDKSDKPNLNGNF
                     NFIKLDLSSDLSPLFTMVPTVDILCNTAGILDAYKPLLEVSDEELEHLFDINFFVTVR
                     LTRHYLRRMVEKKSGIIINMCSIASFIAGGGGAAYTSSKHALAGFTRQLALDYAKDCI
                     QIFGIAPGAVQTAMTASDFEPGGLAEWVASETPIGRWTKPSEVAELTGFLASGKARSM
                     QGEIVKIDGGWSLK"
     gene            complement(10779..11015)
                     /locus_tag="VBC94_001404"
     CDS             complement(10779..11015)
                     /locus_tag="VBC94_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEO6979669.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGDAENYVQLFDTLEVNGKVLQAT
                     PYFLINVTGEGEGFSMWAPTPCDVLAEDWIEVND"
     gene            complement(11105..>11312)
                     /locus_tag="VBC94_001405"
     CDS             complement(11105..>11312)
                     /locus_tag="VBC94_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979670.1"
                     /translation="LNQLSISYELVENGPVLLTEQADNFIVGIEGVRTKIMFLTNKKK
                     TKYYLLIMDDQKLFYPMKTIVAFR"
BASE COUNT         3693 a         2301 c         1611 g         3707 t
ORIGIN      
        1 atcttaataa cttaatcaag gagtatgtat gaaaaaaatt atttatcttg gtttagcctg
       61 tgtcagtata ttaactttaa gtggttgcga gtcaattgag cgttctctaa aaggagaccg
      121 ttatgttgac caaaaactag ctgaaaactc ttcaaaggaa gctactgaac aactaaacaa
      181 aaaaactaag caagccttaa aagctgataa aaaagctttt cctcaattgg acaaggctgt
      241 tgccaaaaac gaggcacaag tgcttataaa aacctctaaa ggtgatataa atatcaagtt
      301 atttccaaaa tatgctcctt tagctgttga aaattttcta acacatgcaa aagaaggata
      361 ctataatggg ttaagtttcc acagagttat caaagatttt atgatacaat ctggtgaccc
      421 taatggagac gggaccggtg gaaaatctat atggaatagc aaagataaaa agaaagattc
      481 tggaaatggt tttgttaatg aaatatcccc ttacctctac aatatcaggg gaagtttagc
      541 aatggcaaat gctggagctg atactaatgg cagccaattc tttatcaatc aaagccagca
      601 agatcattcc aaacaactgt cagataaaaa agttcctaag gtgattatta aagcctattc
      661 agagggagga aatccaagtt tagatggtgg ttataccgtc tttggacaag taatctctgg
      721 catggaaact gtggataaaa ttgcttcggt agaagttaca aaatcagatc aaccaaaaga
      781 aaaaattact attacaagta tcaaagttat taaagactac aaatttaaat aattctatca
      841 aaaatgagac tagaacattt tctagtctca ttttttaaca acactattgt ttctttttaa
      901 acaaactttg tttaggagaa aataggaaag caagtaaaaa cataaaagta gatgttaaaa
      961 caatacttga tcctgcggca atattaaatg tataaccgat atataacccc agaacagatg
     1021 ctactgctcc taatgcagat gataaaaaca acattgttct gagactttta acataaagat
     1081 atgctgttgc agctggtgta attaaaagtg ctacgatcaa tattgttccc acactctgca
     1141 tagctgttac tgcaactaat gttaacaaaa tcatcaataa ataatgataa aaagaaactc
     1201 tcattcccat tgattttgct aaaacaggat caaaagaagt caataataat tctttaaaga
     1261 agatagtaat cagagtcagt acaattaatc ccacaataat agtcatatat ttatcactat
     1321 cttgtactgc caaaatattc ccaaaaagaa tatgaaagag gtcagttgta ctattagcaa
     1381 gtccaattaa aatgatacct aaggctaaaa aggacgaaaa agtaattcca atagcagtat
     1441 ctccttttat gacactattt tctttaatat aagtaatgat aatcgaagac aacaaaccaa
     1501 agacaatagc acctataaaa aagttaatcc ctaaaataaa agatatagca acacctggta
     1561 gaacagcgtg agaaatagca tcacccatca aagacattga gcgcaagata ataaaacagc
     1621 ctactgcacc cgctacaata ccaatcacaa ttgcagttat aaaagcattt tgcaaaaaat
     1681 gataagtcaa taagccttca aaaaatttcg taaacattct agtctccttg tcctaacaaa
     1741 atagcatcac cgtaagcagc acttaaattc tcacgggtaa atgcctgatc tattggtcca
     1801 caagcaatta gatgacggtt caaaataatt acttgatcaa aataatgatc aactttactc
     1861 aaatcgtgat ggacgactaa aattaatttt ccggccttac ttaatttttt tagtaaatta
     1921 actatgattt gttcactaat ggaatcaatc cctacaaacg gctcatctaa aaaaatgtaa
     1981 tccgcttctt gaacgagaca tcttgccata agcatacgtt gaaattgtcc acctgataag
     2041 gcattaattg gtctattttc aaaccctctc aaaccaactt gatcaatcac tcgggatact
     2101 ttttcccagt cagtttttga tagtcgcttg aagaggcctc gttccttata taatcctaaa
     2161 gaaacgcatt ctccaacagt tatagggaat tggtaatcaa tattagtttt ttgttcaaca
     2221 tatgctactc tccccattaa aggtagcaaa tctcccccaa tccctgattc tccagtagaa
     2281 tcaaccaaac caagaagagc tttcataagc gtagatttcc ccgccccatt aggccctaaa
     2341 ataccaatga ttccggagcc ttctaagcgt aaattaatat cttctagcac taaattatta
     2401 tcataactta cgcttaagtg tttactaata atcatttttc ctccaaaagt agaatggagc
     2461 tggttataaa accagactcc acttctctgt atttattaaa gctaagtcag tttttagatt
     2521 aggatttcga cattgttaca actcagcctt aagcgagaat actacatcat tgatttcatt
     2581 atgatttcaa tgtcgattct cactcttatt ttgctaaacc ttctgcaatc ttatcaagat
     2641 tccatttcat catagcgtag tagctgtcac ccttttgacc ttttttagca attgagtctg
     2701 tgaagatttc tgcgtaaatt gggatacctg attctcttga aacagatttc ataggacgct
     2761 tgtcaacact tgattcaacg aataaagctg aaggtcttac ttgttttaat ttcttaacta
     2821 agctagtaat ttgatctggt gtaccttctt cttcagtgtt aatttcccaa atgtatgctg
     2881 atggaactcc ataagctttt gagaaatatt taaagcaacc ttcactagta acaattaatt
     2941 ttttattagc tggaatggca ttaaatttag attttgcttc tttatccaat ttttctaatt
     3001 tagctacata agcatctctg tttttctcat aagtagcttt gttttttgga tctttagcaa
     3061 ttaattgctt agcaatattt tttgagtaaa taataccatt ttcaaggttc aaccaagcat
     3121 gtgggtcttc tttacctttt ccgctttgac catttagata aataacatca actccatcgc
     3181 taaccgcaaa gtaatctttg ttttctttct tcttagcgtt ctttattaat tttgtaaacc
     3241 aagcattacc gcctgtttca agattaattc cgttgtaaaa aacaaggtct gcttgagttg
     3301 ttttttcaac gtctgctggt aatggctcat attcatgagg atctttacca acaggtacaa
     3361 tactatgtaa gtcgattttg ttaccggcta tattttttgt tatatctgcc aaaatagagt
     3421 tggttacaac tacttttaat ttttcgtttt tggcttgatc ctgcttgtta gcacaggcac
     3481 ctaagcctaa aaatgctaca aaacatgata caataactaa ccactttttc atcaaaatgc
     3541 tccttttaaa attaatataa gttatgtatt aaattaagtt aacttaattt tgatattagc
     3601 ataacctatt ataaagtatt tgtcaaatac tttttcagta ttttttgata taatggtctt
     3661 agaaaaatag attttaagaa gtataagaaa gactgaatat gactccaaat aaagaagatt
     3721 acctaaaatg cattcatgaa ttaggtgaaa aaaacaacaa aatttccaat aagaaaatcg
     3781 ctgaaatgat gcaagtctcg gctcctgccg tgtcagaaat gattaaaaaa atgataagtg
     3841 aacaactgat tgttaaagat aaagatttag gatattatct taccaaacaa ggtttattag
     3901 ttgtttctga cctttaccgt aaacatcgtc tagtagaggt atttttagtt aaccatttgc
     3961 actatactgc tgatgatatt catgaagaag cggaagtttt agaacatact gtatcaacaa
     4021 cttttgttga tcaattagaa aagttgttag actttccaca attttgtcca cacggtggga
     4081 ctattcctaa aaagggagag tttttagtag aaattaacca aatgacattg gaccaaatta
     4141 gtcagcttgg cacatatgtt atcagtcgtg ttcatgatga ttttcagcta ctgaaatacc
     4201 tagaacaaca ccgtttgcat atcaacgata ccattgaatt gacacaaatt gacccttatg
     4261 caaaaactta tcacatcacc tataacgatg aaaatctaac aatccctgag cgtatcgcaa
     4321 gtcaaatata tgtatctgct acataaataa aaaagtccgt gtattcatta gatacacggg
     4381 cttttttata atgctttcaa aaatgccatt aatacttggg cagagcgttt accagcttca
     4441 atgataaatt catcaaatgt gatattggca tcatgagctg cagtatcact catagcacgt
     4501 accaccacaa agggctttcc tgtagcttga gcagcttggg caattgctgc accttccatc
     4561 tcgacagcta aaacctgtgg aaaatgcccc ttaataacat caatcttttc ctggccagca
     4621 ataaaactat ccccagttgc aattaaacca actttactag tcatttcttc tttagataaa
     4681 actgcttcaa aagtggaaac aaaagtctta tccgaatgaa aataaagtgg ctgcattgac
     4741 atttgtccat agtcataacc aaaggcggtt aaatctacat cgtgataaac taatgtatct
     4801 gcgacaacga cgtctcctac attcaatcct gttgctactg caccagctga tcctgtatta
     4861 ataatagcat caactttaaa gctttctact aaaatagcaa cactcatcgc tgacatgact
     4921 ttcccaacac cagactgtac taaaacaagc tcatgttcac cataacgtcc actgtaatat
     4981 acatttgata atactgtctc ttgagattta tcctcaagat tttccacaag gagttttaac
     5041 tcttcctcca tggctgcaat aattccaatt ttcatgatat ttccttataa tttaaaaatt
     5101 gcataaaaca agagagccaa caaaataagc aaaatgaata aaactaaatt caacttacgt
     5161 tgaaattcat ttcgttttgc attctcaatc cggcgactct tgtagatatg tttaccgttc
     5221 tcgtcagtta gttgctccat gccatcttct gtagaaataa ttttagtttc ttgatctaag
     5281 attgtttgac ctgaaacttt ttcacctcga tttgaacgtt caatcatatc atctgttaac
     5341 aatggttttc ccattaagaa tgacctccca tttttaaacc gtaatattgt aaagcaatga
     5401 tcgttttcgc atcttgaatc atgccttttt ctaccatttg catacaatcc tcataactta
     5461 actctagaag ctcaaggacc tcatcatcat cttgtggcct aggattttca actttttgta
     5521 aatcagtagc tagatagagg acgattttct cattacaaaa accaatagca gtgtagaaac
     5581 tataaagaat ttcaagattc cctgtgtagc cagtctcttc ttctaattcc cgaagggcag
     5641 cttcttcctt agaacctgac tcacccgtct caagtttccc tgctggaatt tcgtaagaaa
     5701 taccttcaat agccttacga tactgtttca ctaaaacaat tttatgctca ggagttaccg
     5761 ccaaagtagc aacagctcct ccatgaaaaa caagttcacg tttggattat cccaatccat
     5821 ttggcaactc tacatcatca acagctactt tgataatttg tccatcaaaa acagtttgac
     5881 gattaattgt tttttcttca aaatccataa gaacctccca aataagtgtt acttttgtga
     5941 aggatgatgt ggttttttat tggcatatcc ttctttattg acttgacgtc ctcttccaat
     6001 agctatgcta tcaattggaa cattatctgt gatggtagat ccagctgctg ttaaagcatt
     6061 atcaccaatt tcaagtggtg caatcagagt agaattactt ccgataaaga cattacttcc
     6121 gatttctgtt ttaaatttat tttgtccatc ataattgaca gtaatcgtcc ctgcgccaaa
     6181 gttaacatca caacctacct ctgcattgcc gatataagtt aaatgacctg ccttcgtatt
     6241 ctcacctatt tgagaaccct ttacttcaac gaaattacca atatgaacac cttttgcaag
     6301 acttgttcct ggtctaatat gcgcataagg gcctacggtt actccatcag aaataatgga
     6361 ttcttcaacc atcgaattag tgatggtaac atcatttccg acttgagcat ctactaaata
     6421 actaccattg gttaataaag tacctttacc aatcttcgtc tgtcctttta aagtcacatt
     6481 aggctcaatt acagattctt ctccaatctc aacatcaata tcaatataag cggaatcagg
     6541 attaaccact gtgaccccat taaccatatg ttgacgtgca attcggtgac gcatcacttt
     6601 ttcagcagtt gcaagtgcta cacgatcatt aactcctaga ctttcgtcaa agtcacgaag
     6661 tttataggca cctacctttt tacccgcttc tttgaaaatt ccaataacat cagttagata
     6721 atactctcct tgagcgttat ttgtattaat gtctttaagt gcttcaaata gactttgatt
     6781 atcaaaaaca taagttccag tattaatctc tttaacctgc tgctcaaagt cattagcatc
     6841 cttttgttct acaattttag taacttcatc atctgaatta cggataatac gaccatatcc
     6901 aaatggattt gcagcatcgg ctgttaagat tgttgcaacg tttttatgat tgacatgaaa
     6961 atcaattaag ttttttaaac tttctccagt aatcaggggt gtatcacctg caataactag
     7021 tgtatgtcct tttgatgtcg ctaattcttc ttccgccatc attacagcat ggccagttcc
     7081 tagctgttct gtttgcatta caaactctga tttgtcacct aaaacatcac gtacaagctc
     7141 cgccttatgt ccaataacag taacaatttt acttggctca atagcttgca cactacggaa
     7201 aacatgctca agcatggtaa taccagatac tttatgcata acttttggta aatcagattt
     7261 catgcgagtt cccttacccg cagctaaaat aatggcataa tttgacatat taattcctct
     7321 atctctgaat ataattcaac ctattatatc ataatgcctt caacttatca ctaatacccc
     7381 tttaaatccc tattacaata gactttagct aaaatagctt acttcaatta ggaataaaat
     7441 agctaccttt tggtagctat tcaatacatg aaatccgtta attttttctt ctctgtttac
     7501 aatcagtatt tttataaatc tatcattgca atgctcagat ggtaaaaacg attagtttaa
     7561 ctctgttgaa atttgccaca tcacaccaaa ttggtctact aagtttgcgt aagcaggatt
     7621 ccagaaagtt tcttgtaact ctaactgaac ttcttgtgca ctcttcttta atttttcata
     7681 aatttcacgc gcctcctcta cgctacccac aataatagcg gctgtaacat ttttaccagc
     7741 ttgatacacg aaatctggat tttcatccga gatttgaaga cgaatgccat caaaaataag
     7801 ctgagcatta atgattaaat ccttgcggtc ttcaagacag ttggggtcca tctcttccca
     7861 tgtcatcata ttcactaaat ccgcttgaaa gacatcttta taaaaatcaa ccgcttgtcg
     7921 cccatttcca tttgttacga tgtatgtttc tagtgcttta accataagtt ctccttttta
     7981 ttttacttca cggattgcaa agtaattatt gtcattatcg gaaaaattaa aaactctcat
     8041 actccccata tcaacaatag gatttgtcat aacctcattt gctgttaatt cttgatatgt
     8101 actgtcaata tcagtagttt caaataaaat agagggagtt gctaaatcta gctcaggact
     8161 catttgagct ataattgctt tgtcatgtaa gacgaagcta acttcactat ccaacttcgg
     8221 tgcaactata taggttacat aatctccttg tgtctatttt tcaacacgct caaaacccac
     8281 cttgtttttc caaaaatctg ctgatgcttc aacattgcta acgtagagca ttacctgtcc
     8341 aattgatgaa atcattttat tatctcactt tcggttttat tactaattag tataacatac
     8401 agtatttaaa ataagtaaaa aatgactcaa aagagtcatt tacttatttc gttaccatat
     8461 ctagaacttt catcaccgtt ctactatgtt ctaaagcttg agcagcacgc ttaaaatcta
     8521 aattgctaat aataccttca aaaataacaa attcatcgtg catgcggtgc ctatcaccat
     8581 ttaattgata aacatgactt tcttgtccat tcatagttaa tgaaatttta ggcatagtat
     8641 tagttggccc taaaatcgct atagacccct tatcaccttg aatagtagat tttacttctg
     8701 cactacaatc ttttgctcca atacagactg ttttaaagtg cccatagtct aggactagaa
     8761 ctcctgaagt atcaatacca cgctctatat ttggtaaata ctgtgctgtt ataggttctc
     8821 caaaaagtcc aattactaaa tgtaaattat aaatattaag gtctctcaac gcacctccac
     8881 ccatttcagg attgaaagct ggtgctattt ccccacgttt aaaggcatca taacgcgatg
     8941 aatattggga ataattacat tctacaattt taatatctcc aaggttgctt aaatgttctt
     9001 ttacaaggtc aaaattaggt aaatattgat ttgtaattgc ttcgagtaat attagttgcc
     9061 tagtattggc aatagataca agttcttcta attgacttgc ttctaatgta aatggcttct
     9121 cacaaatgac atgttttcct gccaagagtg cctccttagc atagtcaaaa tgcaagtgat
     9181 tcggcagtcc aatatagact gtgtcaatct cctcattgtc caagacagcc ttatattcac
     9241 tcgtagctag agacatgtta tattccttag ctagatcttt agctgtctcc aaacttctaa
     9301 ctgttgataa gatagcaact agatcaatac cttcaatttt ctgcaagaca ggcaaaactt
     9361 ccttaacaat catacctgtc cccaaaaccg ctaatttcat tcgttaatct cctttgggta
     9421 aaccacttgg aattcctcta aaactatcct agagtcctca ctaaacatta aaccaaactc
     9481 ctcaaaataa ggtttaaaga aatcaatgtg agcttttctc caccattcta aagttttatc
     9541 tccttctcct tctttaaaag catggtctgc agagacatct ttaaaaggga caatatcaac
     9601 tttagttatt tcaataatac agatagcttc atcattgcta tccaaaataa tatcatatga
     9661 accaacttgt ggtaaccctt cctcatctaa ttcatataaa tcataggcag aggccgttgc
     9721 agtttttgtc ccatctacaa ctaactttgc cagaagatct ggttctgctc caaattgcca
     9781 tgcgtctatc tcatcaccga ttgaaggatt caaagattta aacttattcc ataattcttg
     9841 tgccttcata ttacctccga agcaattccc acaacaaact taactgcgtt actcattttt
     9901 ctttgtcttt taaatccttc tttaacatct aaaatataaa cagtttgacc aatccaaaca
     9961 cgtaaataaa cagattttat taaaatatca cctgatattc ctttaacttc ccgctcagta
    10021 ccatcagatg cttcaaattc cgtacgtaat aaccaagtat taccacaacc aaattcaatc
    10081 tattttctca tttcaaactc cagccaccat caattttgac aatttcacct tgcattgatc
    10141 tcgcttttcc tgaagcaaga aatccagtta attcagcaac ttcacttggt ttagtccaac
    10201 gaccaattgg cgtctcacta gctacccatt ctgctaatcc accaggttca aaatcactag
    10261 cagtcatagc agtttgtaca gcacctggcg ctataccaaa aatttgaata caatcttttg
    10321 cgtaatccaa agccaattga cgtgtgaatc ccgcaagggc atgtttagaa ctagtatatg
    10381 cagctccacc gccacccgct ataaaacttg ctattgagca catattgata ataatacctg
    10441 acttcttttc caccatacgt ctaagataat gacgtgttag cctaacagtc acgaaaaaat
    10501 taatatcaaa taggtgttcc agttcttcat cacttacttc tagaagtggc ttgtaggcat
    10561 ctagaatacc tgcagtatta cacaaaatat caacagtcgg taccattgtg aatagtgggg
    10621 ataaatcaga ggataaatcc aactttataa agttaaaatt accatttaag ttcggtttat
    10681 ccgacttatc cacaccatat acttgatacc cttgtttcaa aaaatattga gcctgtgcat
    10741 aaccaattcc actagcacag cccgtcacta aaacaacttt agtcattgac ctcgatccaa
    10801 tcctctgcta aaacatcaca tggtgttggt gcccacatgg aaaaaccttc accttcacca
    10861 gtaacattaa taagaaaata aggtgtagcc tgtagtactt tcccattaac ttcaagggta
    10921 tcaaataact gaacataatt ttcagcgtcc ccccaacctg tacgtacata ttttttcttt
    10981 gcttttaaac ctggtaaaat ttcttcaaat gtcatattgt tctcctttat tgtctttaac
    11041 cattgatata tcaaggttta ttgcttattt aaaatcataa tttaaagttt cttcgccttt
    11101 tagttcatct aaaggctact attgttttca taggataaaa tagtttctgg tcatccatta
    11161 ttagtaaata atatttcgtc tttttcttgt tggtcaagaa cattattttt gttcttactc
    11221 cttcaattcc cacaataaag ttatctgctt gctccgtcaa caaaacaggt ccattttcca
    11281 ctaattcata agaaatactt agttgattta gc
//
LOCUS       DATROQ010000058        11284 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.059, whole genome shotgun sequence.
ACCESSION   DATROQ010000058 DATROQ010000000
VERSION     DATROQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11284
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.059"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            156..866
                     /locus_tag="VBC94_001406"
     CDS             156..866
                     /locus_tag="VBC94_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6979671.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYETATAFDGREALVQYAEF
                     QPDLIILDLMLPELDGLEVAKEVRKTSHIPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTENIETAVAEESAQNASSDITIGELQILPDAFIAKKRGEEIE
                     LTHREFELLHHLATHIGQVMTREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPGR
                     PEYILTRRGVGYYMKSYE"
     gene            859..2208
                     /gene="vicK"
                     /locus_tag="VBC94_001407"
     CDS             859..2208
                     /gene="vicK"
                     /locus_tag="VBC94_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEO6979672.1"
                     /translation="MNNSAANIRSFELALLFLLVFVAVYFVYLAVRDFKMSKDIRLLN
                     WKVRDLIAGNYSDSILIQGDADLVELGESLNDLSDVFRMAHDNLEQEKNRLASILTYM
                     TDGVLATDRSGKIVMINETAQQQFNLAYDEALSMNIVDMLGSGSPYSFQDLVSKTPEV
                     VLNRRDENGEFVTLRIRFALNRRESGFISGLVAVSHDATEQEKEERERRLFVSNVSHE
                     LRTPLTSVKSYLEALDEGALNEEVAPSFIKVSLDETNRMMRMISDLLSLSRIDNEVTH
                     LDVEMTNFTAFMTSILNRFDQIRNQKTVTGKVYEIVRDYPLKSIWVEIDTDKMTQVID
                     NILNNAVKYSPDGGKITVNLRTTKTQMILSISDQGLGIPKKDLPLIFDRFYRVDKARS
                     RQQGGTGLGLSIAKEIVKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVTYEEWEDVED"
     gene            2212..3021
                     /locus_tag="VBC94_001408"
     CDS             2212..3021
                     /locus_tag="VBC94_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6979673.1"
                     /translation="MSEIGFKYSILASGSTGNCFYIETPQKRLLIDAGLTGKKVTSLL
                     AEINRKPEDLDAILVTHEHSDHIKGVGVLARKYHLDIYANEQTWKVMDERNMLGKVDV
                     SQKHVFGRGKTLTFGDLDIESFGVSHDAVDPQFYRMMKDDKSFVMLTDTGYVSDRMAS
                     LIENADGYLIESNHDIEILRSGSYPWTLKQRILSDKGHLSNEDGSETMIRTIGNRTKH
                     IYLGHLSKENNIKELAHMTMENNLMRADFGVGTDFSVHDTSPDSATPLTRI"
     gene            3024..3392
                     /locus_tag="VBC94_001409"
     CDS             3024..3392
                     /locus_tag="VBC94_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEO6979674.1"
                     /translation="MKKIFYISLGFISLGIGIAGIVLPVVPTTPLVLLSAFCFSRSSE
                     KFDIWLRQTKVYKYYAADFVESRSIAPARKKSMIWQIYILMGISIYFAPLMWLKLGLL
                     IGTIVGTYVLFYVVPDKIDE"
     gene            3567..4253
                     /locus_tag="VBC94_001410"
     CDS             3567..4253
                     /locus_tag="VBC94_001410"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEO6979675.1"
                     /translation="MKELRSKLEKDYGIVFANQELLDTAFTHTSYANEHRLLNISHNE
                     RLEFLGDAVLQLLISQYLFTKYPQKAEGDLSKLRSMIVREESLAGFSRLCGFDHYIKL
                     GKGEEKSGGRNRDTILGDLFEAFLGALLLDKGVEVVHAFVNKVMIPHVEKGTYERVKD
                     YKTSLQELLQSHGDVKIDYQVTNESGPAHAKEFEVTVSVNQENLSQGIGRSKKAAEQD
                     AAKNALATLQ"
     gene            4261..7800
                     /gene="smc"
                     /locus_tag="VBC94_001411"
     CDS             4261..7800
                     /gene="smc"
                     /locus_tag="VBC94_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEO6979676.1"
                     /translation="MFLKEIEMQGFKSFADKTKVEFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSAKSLRGGKMPDVIFAGTENRKPLNYAQVSVTLDNSDHFIENIADEVRVER
                     RIFRNGDSEYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEAIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQSKLEQTQGNLDRLEDIIYELDMQVQPLEKQASIAKRFLVL
                     DEERQGLHLSILIEDILQHQSDLTTVEEKLLTVREELATYYQQRQSLEDENQSLKQKR
                     HHLSEEIEVKQLALLDVTKLKSDLERQIDLIRLESSQKAEKKEEAGQRLAELEVKAKD
                     CNDQITQKNIELTTLSEKIAQIRSEIVSIESSLERFSTNPDQVIEKLREDFVTLMQEE
                     ADTSNALTALLADIENQKQASQAKSQEIQEVSKNLEVLKSNAKVALERFEAAKKNVRQ
                     LLSHYQDLGQTLQNLEGEYKNQQSILFDHLDEIKSKQARISSLESILKNHSNFYAGVK
                     SVLQAKDQLGGIIGAVSEHLSFDKHYQTALEIALGGSSQHIIVEDESAAKRSIAFLKK
                     NRQGRATFLPLTTIKPRELAQHYLSKLQSSQGFLGIASELVTYDQRLSNIFKNNLGLT
                     AIFDTVDNANVAARQLNYQVRLVTLDGTELRPGGSYSGGANRQNNTVFIKPELDNLKK
                     ELKQAQSKQLIQEKEVAALLEQLKEKQETLAQLKNDGEQARLEEQRADIEYQQLSEKL
                     ADLNKLYNGLQLSSGALEQTTSENEKNRLEKELEQFAIKKEELTTSIAQIKEDKDSIQ
                     EKVNNLTTLLSEAQLEERDLLNEQKFERANCTRLEITLSEIKRDISNLQTLLSHQDSQ
                     LDKEELPRIEKQLLQANKRRENDEEKLVSLRFELEDCEAALDDLAASLAKEGQKNESL
                     IRQQAQLESQCEQLSQQLMIFSRQLSEDYQMTLDEAKVKANVLEDILMAREQLKSLQA
                     KIKALGPVNIDAIAQFEEVHERLTFLNTQRDDLVHAKNLLLETITDMDDEVKTRFKST
                     FEAIRHSFKETFVQMFGGGSANLILTEGDLLSAGVDISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKSSQFIVVTHRKGT
                     MSAADSIYGVTMQESGVSKIVSVKLKEAQEMTN"
     gene            7891..8688
                     /locus_tag="VBC94_001412"
     CDS             7891..8688
                     /locus_tag="VBC94_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6979677.1"
                     /translation="MIKLVATDMDGTFLDENGTYDKKRLANVLKKFKEQGIVFTAASG
                     RSLLSLEQLFADFRDQMAFIAENGSAAVLFNRLAYEQHLSREQYLDIIDHLSKSPYME
                     NNEYVLSGKDGAYILSDANPDYIEFITHYYDNLQKVSHFEDVDDIVFKVTANFTEETV
                     RQAEEWVNQAIPYATAVTTGFKSIDIILSSVNKRNGLEHLCEQYGIRAEEVLSFGDNI
                     NDLEMLEWSGKAIATENARPEVKEIADCIIGHHNDQAVMAYLESMVD"
     gene            8688..9512
                     /locus_tag="VBC94_001413"
     CDS             8688..9512
                     /locus_tag="VBC94_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6979678.1"
                     /translation="MLDIKILALDLDGTLFTTDKKVSEENKVALKAAREKGIKVVITT
                     GRPLKAIGNLLEDLELVSDEDYSITFNGGLVQQNTGKILAKTAMTRQEVEDIHEELYQ
                     VGLPTDILSEGTVYSIANKGHHSQYHLANPLLEFIEVDDLEQVPKDVVYNKIVSVIDA
                     TYLDQQIAKLPDRLKVDYEMFKSRDIILELMPKGVHKAVGLELLTKHLGLDSSQVMAM
                     GDEANDLSMLEWAGLGVAMANGIPEAKATAKATTICNNDESGVAEAVGKYILSEEN"
     gene            9512..11122
                     /gene="ftsY"
                     /locus_tag="VBC94_001414"
     CDS             9512..11122
                     /gene="ftsY"
                     /locus_tag="VBC94_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEO6979679.1"
                     /translation="MGLFDRLFGHKKKDKELEIEASESVVLENEDSVIDKEESSNFSK
                     ESTLNRTSEVPVAEDDSFLELERDTALSESHQPVTSEIHPLESEDTSEIPVKEDDSFL
                     ELEDRAKTKVADTSEVGNVVPDSTTLSDNVSAKSEASFSDEEQSSDSQASEQFSETPL
                     QEEMSSGKTEVQTESEDTSAADAFLADYYAKRKAIEKEISSNSLSTDESELSEAQEVL
                     SQSQADTIKAESQEEKYNRSLKKTRTGFSARLNAFLSNFRRVDEEFFEELEEMLILSD
                     VGVNVATQLTEDLRYEAKLENAKKSEDLKRVIVEKLVEIYEKDGIYNEAINFQEGLTV
                     MLFVGVNGVGKTTSIGKLAHQYKSQGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVVT
                     GEKKADPASVVFDGMEKAVAQGVDVLLIDTAGRLQNKENLMAELEKIGRIIKRVVPDA
                     PHETLLALDASTGQNALSQAKEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVK
                     FIGFGEKIDDIGEFNSEDFMRGLLEGIL"
BASE COUNT         3851 a         1700 c         2373 g         3360 t
ORIGIN      
        1 taataggatg tattgaaagt ttccaattaa ctaggaaatc tgttataatt agtgataata
       61 gtggtgcttt aggcccatat tatcatattt tcggtcactt ttatttttca gtttatggat
      121 ggaagtggga gaccagaaag taatagaggt aaaatatgaa aaaaatactt atcgttgatg
      181 atgaaaaacc aatttcagat attatcaaat ttaatttaac caaagaagga tatgaaacgg
      241 cgacagcttt cgatggccgt gaagctttag tacaatatgc agaatttcaa ccagatttaa
      301 tcattttgga tttaatgctc cctgaactag atggtttaga agtggctaag gaagttcgta
      361 aaaccagcca tattcctatt attatgcttt ccgcaaaaga cagtgagttt gacaaagtca
      421 ttggtttaga aatcggtgca gatgattatg ttactaaacc tttttcaaat cgtgaacttt
      481 tggcacgggt caaagcacat ttacgacgta ctgaaaatat tgaaacagct gtagcagaag
      541 aaagtgctca aaatgcttct tcagatatta ctattggtga gttgcaaatt ttaccagacg
      601 cctttattgc taagaaacgt ggcgaagaaa ttgaattaac gcaccgagaa tttgaattac
      661 ttcaccattt agcgacacat ataggacaag tcatgacacg cgaacattta ttggaaacgg
      721 tttggggata tgattatttt ggagatgtcc gtacagttga tgtgacggtg agacgtttac
      781 gtgaaaagat tgaagatact cctggtcgtc cagaatatat tttgactcga cgtggtgtag
      841 gttattacat gaagtcttat gaataatagt gctgcaaata ttaggtcgtt tgagctagcc
      901 ttactttttt tactagtttt tgttgctgtc tattttgttt atctagcggt tagagatttt
      961 aaaatgtcta aagatatccg gcttttgaat tggaaagtca gagatttgat agcaggaaat
     1021 tattcagata gtattctaat tcaaggtgat gcagatttgg tagaattagg ggaaagtcta
     1081 aatgatttat ctgatgtctt ccgaatggca catgataact tagaacaaga aaagaatcgc
     1141 ttagcgagta tcttaactta tatgactgac ggtgtgctgg ctacagatcg ttcaggaaaa
     1201 atcgttatga ttaatgagac agcccaacag cagttcaatc tagcatatga tgaagcttta
     1261 tcaatgaata ttgttgatat gttagggtcg ggaagtccat attcgttcca agatttagtc
     1321 tctaagacgc cagaggtagt tcttaataga cgtgatgaaa atggtgaatt tgttacctta
     1381 cggattcgat ttgctttgaa ccgtcgtgaa agtggtttta tttccggttt agttgcagta
     1441 tcacacgatg ctactgagca agagaaagaa gagcgtgaaa gaagactttt tgtatctaat
     1501 gtttctcatg aacttcgtac acctttgaca tctgtaaaat cttatttaga agctttagat
     1561 gagggagcgc tcaatgaaga agttgctcca agcttcatta aggtatctct agatgaaaca
     1621 aatcgtatga tgcgtatgat atcagattta ttaagtttat cgcgcattga taatgaagta
     1681 acgcatttag atgttgaaat gacgaatttt acagctttca tgacctcaat tttgaatcga
     1741 tttgatcaga ttagaaatca aaaaacagtc acaggaaaag tttatgaaat tgtcagagat
     1801 tatcctctta agtcaatttg ggtggaaatt gatacagata agatgactca agtgattgat
     1861 aacattttaa ataatgcagt caagtattca ccagatggtg gtaagattac agttaatcta
     1921 cgcacaacta aaacgcagat gattttatca atatcagacc aaggcttagg tattcccaaa
     1981 aaagatttac ctctcatttt tgatcgtttt tatcgtgttg ataaggcgag aagtcgtcaa
     2041 cagggtggga ctggacttgg tttgtcaatt gcaaaagaaa ttgttaagca gcataaggga
     2101 tttatttggg ctaagagtga gtatggtaaa gggtctactt ttacaatcgt cttgccttat
     2161 gataaagatg ctgtaactta tgaagaatgg gaggacgttg aagattaaaa catgtcagaa
     2221 ataggattta aatatagtat cttagcttca ggttcaactg gaaattgttt ttatattgag
     2281 acaccccaaa aacgtctttt aattgacgct ggtttaacag gcaagaaagt gactagcctt
     2341 cttgcagaaa ttaatcgtaa accagaagat ttagatgcta tactagttac ccatgaacat
     2401 tctgaccaca ttaaaggtgt tggcgtattg gctaggaaat atcaccttga tatttatgcc
     2461 aatgagcaga cgtggaaagt tatggatgag cgtaacatgt taggcaaagt tgatgttagt
     2521 caaaagcatg tttttggacg cggtaaaacc ttaactttcg gggatttaga tattgagagc
     2581 tttggtgtaa gtcatgatgc tgtggatccc caattttacc gtatgatgaa agatgataag
     2641 agctttgtga tgttgacaga tacaggatat gttagcgacc gaatggcaag cttgattgag
     2701 aatgcagatg gttatttgat cgagtcaaat cacgacattg agattctacg ttcgggttct
     2761 tatccttgga cacttaagca acgtatttta tcagataaag gacatttatc taatgaagat
     2821 ggttcagaaa ccatgattcg cactataggt aaccgtacta aacacatcta tttaggacac
     2881 ttgagtaagg aaaataatat taaggaactt gctcacatga ctatggaaaa caatttaatg
     2941 agagctgact ttggtgtggg cacagatttt tctgttcacg atacttcacc tgattcagct
     3001 accccactga cgaggattta agattgaaaa agatttttta cattagttta ggatttatta
     3061 gtttagggat aggcattgca ggtattgtct tgccagtagt tccaacgact ccattagtat
     3121 tgctttcagc tttttgcttt tcgagaagct ctgaaaaatt tgatatttgg ttgcgtcaaa
     3181 ctaaagttta taaatactac gctgcagatt ttgttgaaag cagaagtatt gccccagcgc
     3241 gtaaaaagtc aatgatttgg caaatttata tcttgatggg gatttcaata tattttgctc
     3301 ctctaatgtg gctcaaactg ggattattga ttggaactat tgtaggaact tatgtgcttt
     3361 tttatgtagt acctgataaa attgatgaat agttaacaag agactaggtg tgtatcctag
     3421 tcttttgttg aatattcaaa tatgtaatta aggaaacgtt ttctttggaa gataagttta
     3481 acttccaaaa tgtttgatta tttgtcaact gtggtataat ggtatttgtt taatatttta
     3541 tctatgattg aaatcaagga gaaaaaatga aagaattaag atcaaagctg gaaaaagact
     3601 atggtattgt ttttgctaat caagaattat tagatacagc ttttacccat acatcatacg
     3661 caaatgagca tcgcctccta aacatttcac ataatgaacg tttggaattt ttaggagacg
     3721 ctgttctaca attacttatt tcacagtatc tttttacaaa gtaccctcaa aaggcagaag
     3781 gtgatttatc aaaattacgt tcaatgattg ttcgtgagga aagtcttgca ggtttttcac
     3841 gtctatgtgg ctttgatcac tatattaaac ttggaaaagg agaagaaaaa tctggtggac
     3901 gtaatcgtga cactatttta ggagatttat ttgaggcttt tttaggagct ttattattgg
     3961 ataaaggtgt tgaagtggtt catgcctttg ttaacaaggt aatgatccct cacgttgaaa
     4021 aagggaccta tgagcgtgtt aaagattaca agacaagctt gcaagaatta cttcaatctc
     4081 atggggatgt aaaaatagat tatcaagtta ctaatgaaag tggtccagca catgcaaagg
     4141 aatttgaggt gactgtatct gtcaaccaag aaaatttgag ccaaggaatt ggacgatcaa
     4201 agaaagctgc tgaacaagac gctgccaaaa atgctttagc aacgttacaa tgaggaaatc
     4261 atgtttttaa aagaaattga aatgcaaggc tttaagtcct ttgcggataa aactaaggta
     4321 gaatttgatc aaggagttac tgctgttgta ggtccaaatg gatcaggtaa aagtaatatc
     4381 acagagagtt tacgttgggc attgggagaa tcaagcgcaa aaagtttacg aggaggtaag
     4441 atgccagacg tcatatttgc tggtacagaa aatcgtaagc cccttaatta tgcgcaagtg
     4501 agtgttactt tagataacag cgaccacttt atcgaaaata tagctgacga agttcgagtt
     4561 gaacgtcgta tttttcgaaa cggagatagt gaatatttaa tagatggacg caaagttcgc
     4621 ttacgtgata ttcatgattt atttatggat actggattag ggagagattc tttttctatt
     4681 atttcccaag gacgcgttga agctattttt aatagtaaac cagaagagcg ccgtgccata
     4741 tttgaagaag ccgctggtgt tctgaaatac aaaacacgta agaaagaaac acaatccaaa
     4801 ttagaacaaa ctcaagggaa cttagatcgc ttagaggata ttatttatga attggacatg
     4861 caagtccaac cattagaaaa acaagcaagt attgctaagc gttttttagt attagatgag
     4921 gaacgacaag gactccactt atctattttg attgaagata ttttacaaca tcaatcagat
     4981 ttaactactg ttgaggaaaa gctattgaca gtaagagaag agttagcaac ttattaccaa
     5041 caacgtcagt cccttgaaga tgaaaatcaa tcgttaaaac agaaacgtca ccatttatct
     5101 gaagaaattg aggttaaaca gttagctctt ttagacgtta caaagttaaa gtcagatttg
     5161 gaacgccaaa ttgatctgat tcgtttagag tcaagccaaa aggctgaaaa gaaagaagag
     5221 gcaggtcaac gcttagcaga gttagaagtt aaagctaaag attgtaatga ccagattacc
     5281 caaaaaaata tagagttaac tactctgtcg gaaaaaatag cccaaataag atcagaaatt
     5341 gttagtatag aaagttcctt agagcgtttt tcaactaatc cggatcaggt tattgaaaaa
     5401 cttcgagaag actttgttac cttgatgcaa gaagaagccg acacttcaaa cgctttaaca
     5461 gctttactag ctgatataga gaatcaaaaa caagcttctc aagctaaatc tcaagaaata
     5521 caagaagtta gcaaaaattt agaagtatta aaaagtaacg caaaggtagc attggaacga
     5581 tttgaggctg caaagaagaa tgttcgtcag ttattatctc actaccaaga tttaggtcaa
     5641 accttgcaaa atctagaagg tgagtataag aatcagcagt caattttatt tgatcattta
     5701 gatgaaatca aatcaaagca agcacgtatt tcgagtttag aatctatttt gaaaaatcat
     5761 agtaatttct atgctggagt gaagtcagtt ctacaggcta aggaccagct tggaggtatc
     5821 ataggtgctg ttagtgagca tcttagcttt gacaaacatt accaaacagc attggagata
     5881 gctttgggag gtagcagcca acacatcatt gttgaagacg agagtgctgc caaacgttca
     5941 attgcttttt taaagaaaaa ccgccaaggt agagcgacat ttctgccttt aactacaata
     6001 aaaccaagag aattagcaca acattatctc tcaaaattac aaagttctca agggttttta
     6061 ggaatagcta gtgaattggt aacctatgat caacgcttgt caaacatttt taaaaataac
     6121 ttagggttaa ctgctatttt tgatacggtt gataatgcta atgtagctgc aagacaattg
     6181 aattatcaag tacgtttagt aaccttagat ggaacagagt tacgccctgg agggtcgtac
     6241 tcagggggag caaatcgtca aaataatact gtttttatca agcctgaatt ggataatcta
     6301 aaaaaagaat tgaaacaggc acaaagtaaa cagttaattc aagaaaaaga agttgccgca
     6361 ttgttagagc aactcaaaga gaaacaagaa accttagcac aacttaagaa tgatggagaa
     6421 caagctcgtc ttgaggaaca aagagctgat atagagtatc aacaactatc agaaaagctt
     6481 gctgatttaa ataaactata taatggcttg caattaagca gcggggcgtt ggaacaaact
     6541 acttctgaaa atgaaaaaaa tcgtttagaa aaagaacttg aacaatttgc tattaaaaaa
     6601 gaagagttga caacttcaat tgctcaaatt aaggaagata aagattcaat tcaagaaaaa
     6661 gtcaataatc taacaacatt attgtcagag gcacagctag aagaacgtga tttgctaaac
     6721 gagcagaaat ttgaacgtgc caactgtaca cgtctagaga ttaccttgtc tgaaataaaa
     6781 cgtgacatta gtaatttaca aacgttacta agccatcagg acagtcaatt agacaaagaa
     6841 gagcttccac gtatagaaaa acagttgcta caagcaaata agagaagaga aaatgacgaa
     6901 gaaaagcttg tgagtctccg ttttgaattg gaggattgtg aagctgcttt agatgattta
     6961 gctgcttctc tggctaaaga aggacaaaaa aatgaaagcc ttattcgtca gcaagctcaa
     7021 ttagagtcac aatgtgaaca attatctcaa caattgatga tattctcacg tcaattatca
     7081 gaagactatc agatgacatt ggatgaagcg aaggtaaaag caaatgtctt ggaagatatt
     7141 ttgatggcgc gtgaacaatt gaaatctcta caagctaaaa ttaaagcttt aggacctgtt
     7201 aatattgatg caattgctca atttgaagaa gttcatgaac gattgacctt ccttaacaca
     7261 cagcgagacg acttagttca tgccaaaaat ttattactag agacgattac agatatggat
     7321 gatgaagtta agactcgttt taaatcgaca tttgaagcca ttcgtcatag tttcaaagaa
     7381 acctttgtac agatgtttgg tggaggttct gcgaacctta ttctgacaga aggtgattta
     7441 ttatcagctg gggttgatat ttcggttcaa cctcctggta aaaaaatcca gtctttaaac
     7501 ttgatgtcag gtggtgaaaa agcattatca gcgttagcat tattatttgc cattatccgt
     7561 gttaagacaa ttccatttgt catcttggat gaagttgagg ccgctttaga tgaagcaaat
     7621 gttaagcgat ttggcgatta tcttaatcga tttgataaat caagtcaatt tatcgttgtt
     7681 actcaccgaa aagggactat gtctgctgct gatagtatct atggggtaac gatgcaggaa
     7741 tcaggcgttt ccaaaattgt ttctgtaaaa ttaaaagaag ctcaagaaat gaccaattag
     7801 aaaacagaag gggatccctt ctgttttttg gattttcagc cttattttat gttacaatag
     7861 taaatgtaaa ccctttagtt aggagattcc atgataaaat tagtagcaac cgatatggac
     7921 ggtacattct tagatgaaaa cggtacctat gataaaaaac gtttagcaaa tgtgctaaaa
     7981 aaatttaaag aacaaggaat tgtctttacg gcagcaagtg ggcgttcctt attatcactt
     8041 gaacaattat ttgctgactt tcgtgatcag atggctttta tagcagaaaa cggaagtgca
     8101 gcagtcttat ttaatcgctt ggcatatgaa cagcacttga gtcgagaaca atacttagat
     8161 attattgacc atttgagtaa gagcccttat atggagaata atgaatatgt cttatctggc
     8221 aaggatggtg catatattct aagtgatgcc aatccagatt atattgaatt catcactcat
     8281 tattatgata atttgcaaaa agtttctcac tttgaagatg ttgatgatat tgttttcaaa
     8341 gtaacggcta actttactga agagactgtt agacaagctg aggagtgggt aaatcaagct
     8401 ataccgtatg ccacagcagt gacaacaggt tttaaatcca ttgatattat tctaagctca
     8461 gttaataaac gaaatggttt agagcacctc tgtgaacaat acggtattag agcagaagaa
     8521 gtcttatctt ttggtgacaa tattaatgat ttagaaatgc tagagtggtc aggaaaagct
     8581 atcgcaacag aaaatgctag accagaagtt aaagagatag cagattgtat tataggtcat
     8641 cacaatgatc aagctgtaat ggcttattta gaaagtatgg tggattaatg ttggatatta
     8701 aaatacttgc cttagattta gatggcactt tatttacaac agataagaaa gtttcagaag
     8761 aaaataaagt ggccttaaaa gcagctagag aaaaaggtat aaaagttgtt attacaacag
     8821 ggcgtccttt aaaagcaatt ggtaatttgt tagaggacct agagttagta tctgatgaag
     8881 attacagcat tacttttaac ggtggtttag ttcaacaaaa tacagggaaa atcttagcta
     8941 aaactgcaat gacacgacaa gaagtggaag atatccatga ggagttgtat caagtgggtt
     9001 tgccaacaga tatcttaagt gagggaacgg tatatagtat tgctaacaag ggacaccatt
     9061 ctcaatatca tttagccaac ccactcttag aatttataga agttgatgat ttagagcaag
     9121 tgccaaagga tgtagtttat aataaaattg ttagtgttat tgatgctaca tatcttgatc
     9181 aacaaattgc taaacttcct gatagactta aagttgacta tgaaatgttt aaatcacgtg
     9241 atatcatttt agaattgatg cctaaaggtg ttcataaagc agtcggctta gaactactga
     9301 caaaacacct tgggttagat agtagtcaag ttatggcaat gggagatgaa gcaaatgatt
     9361 tatcaatgct agagtgggca ggcttaggag ttgcaatggc taatggtatt ccagaagcta
     9421 aggcaacagc aaaagcgaca actatttgta ataacgatga gtctggagtt gccgaagcag
     9481 ttgggaaata tattttaagt gaggaaaatt aatgggatta tttgaccgcc tatttggaca
     9541 taagaaaaaa gataaagaac tagaaattga agcatcagaa tctgttgttt tagaaaatga
     9601 agattcagtt attgataaag aggaaagcag caacttttcg aaggagtcaa ctttgaatcg
     9661 aacctctgaa gtccctgtcg cagaagatga ttctttctta gaattagaaa gagatacagc
     9721 attgtcagaa tcacatcaac cagttacctc tgaaattcat cctttagagt cagaagatac
     9781 ctctgaaatt cctgtaaaag aagacgatag ttttttagaa ctagaagata gggctaagac
     9841 aaaggtggca gatacttctg aggtaggaaa tgtagttcct gattccacga ctctttctga
     9901 caatgtttct gctaaatctg aggcatcttt tagtgatgag gagcaatcaa gtgatagtca
     9961 ggcttcagag cagttttcag aaactcccct tcaagaagag atgtctagtg gaaaaactga
    10021 ggtacaaaca gagtcagagg atacatctgc ggctgatgct tttttagcag attactatgc
    10081 taaacgtaaa gcaattgaga aagaaatctc gtctaattcg ctgtcaacag atgagtcaga
    10141 actttctgag gcacaggaag ttttatcgca atcacaggca gacactataa aagctgagag
    10201 tcaagaggaa aaatataatc gtagcctgaa aaaaacacgc acaggtttta gtgcacgcct
    10261 taatgctttc ctttctaact tccgtcgtgt tgacgaagag ttctttgaag aattagaaga
    10321 aatgttaatc ttatctgacg ttggggtaaa tgttgcaaca cagttaactg aagaccttcg
    10381 ttatgaagca aaattagaga atgccaagaa atcagaggat ttaaaacgtg ttatcgttga
    10441 aaaattagtt gaaatctacg aaaaagatgg tatttataat gaggctatca atttccaaga
    10501 aggtttaacc gtaatgcttt ttgtaggtgt taatggtgtt ggaaaaacga catctatagg
    10561 taagttagct catcaataca agtctcaagg gaaaaaagtt atgttagtag cagctgatac
    10621 tttccgtgca ggtgctgtgg ctcaactagt agaatgggga cgccgtgttg atgtaccagt
    10681 tgtcactgga gaaaaaaaag cagatccggc tagcgttgtc tttgatggca tggaaaaagc
    10741 cgttgctcaa ggcgttgatg tcttgttaat tgatacagca ggacgtttgc aaaataaaga
    10801 aaaccttatg gcagagttgg aaaaaattgg acgtattatt aagcgtgtcg taccagatgc
    10861 tccccacgaa actttgcttg ctttagatgc atctacagga caaaatgctt taagtcaagc
    10921 taaagagttc tcgaaaatta caccattaac tggccttatt ctgactaaaa ttgatggtac
    10981 tgctaagggt ggtgtggtac tggctattcg tcaagaacta gatattcctg ttaaattcat
    11041 tggtttcggc gaaaaaatcg atgatattgg tgaatttaat tcagaagatt tcatgcgtgg
    11101 ccttctggaa ggaattttat aagaaaaaag aactttcatt tagaaagttc tttttatagt
    11161 acgacgggca tgtcgtacat ctgaggtgca agtcctctgt gggcacccgc taccggtgaa
    11221 cccaatagcg actcccaagc ttgactatcg tgaggtagcg gggagaggaa gggatagcga
    11281 aatc
//
LOCUS       DATROQ010000059        11170 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.060, whole genome shotgun sequence.
ACCESSION   DATROQ010000059 DATROQ010000000
VERSION     DATROQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11170
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.060"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            27..419
                     /locus_tag="VBC94_001415"
     CDS             27..419
                     /locus_tag="VBC94_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO6979680.1"
                     /translation="MKLYVQLMVILTFSFAGEVLSTIFNLPIPGSIIGLILLFLALKY
                     KIIRLRHIDTVGNFLLANMTILFLPPAVGLMEHFQDIKPYLFGIAIIILGALFLNILT
                     IGLVSQWIKKRYEGDYPEIGGKNGNFNE"
     gene            400..1095
                     /locus_tag="VBC94_001416"
     CDS             400..1095
                     /locus_tag="VBC94_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEO6979681.1"
                     /translation="MATLTNNPIFGIMLTVWAYYIGIRIFRKYPSPATTPLLLATILL
                     IAFLKLTHISYKDYYNGGSFLTMLITPSTVVLAIPLYRTFHLMKHHIKSISISIILAS
                     VINTVFTAIVAKFFGMKYFLAISLFPKSVTTAMAVGITSKAGGLATITLVVVVITGIL
                     TSVLGPIFLKLLKIEDPVAIGLALGGTGHAIGTGQALKYGQVQGAMAGLAIGITGICY
                     VIVSPLVAGLILK"
     gene            1164..1787
                     /locus_tag="VBC94_001417"
     CDS             1164..1787
                     /locus_tag="VBC94_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEO6979682.1"
                     /translation="MIPLKKITVGTIVCLSFLGLTACSSSNTQQTSTSKSNVSQHKNI
                     KADHEELRLKFNKVKLGVKANNFKGGTSLAELKQLFGGEPNEKFDTPAGNVTLKGYRW
                     NVDDISITIQLLNDSSIVRSISNFKFIRDANITTKDYNSLKNGMSYNKVKELLGEPDD
                     ISQAVSSDKEELQAAWISGIQSSDSDPGINLTFENDKLTNKQQHGLK"
     gene            complement(2056..2127)
                     /locus_tag="VBC94_001418"
     tRNA            complement(2056..2127)
                     /locus_tag="VBC94_001418"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2092..2094),aa:Arg,seq:ccg)
     gene            complement(2200..3402)
                     /gene="rpsA"
                     /locus_tag="VBC94_001419"
     CDS             complement(2200..3402)
                     /gene="rpsA"
                     /locus_tag="VBC94_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEO6979683.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIDGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVVEESAAAARKEVFSNIEVGSVVTGKVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVTVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AAGDVIEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSAGQEVTVKVL
                     EVNSDAERVSLSMKALEERPAQAEGEKEEKRQSRPRRPRRQEKRDYELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(3528..3599)
                     /locus_tag="VBC94_001420"
     tRNA            complement(3528..3599)
                     /locus_tag="VBC94_001420"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3565..3567),aa:Gln,seq:ttg)
     gene            complement(3605..3685)
                     /locus_tag="VBC94_001421"
     tRNA            complement(3605..3685)
                     /locus_tag="VBC94_001421"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3649..3651),aa:Tyr,seq:gta)
     gene            complement(3746..3976)
                     /locus_tag="VBC94_001422"
     CDS             complement(3746..3976)
                     /locus_tag="VBC94_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001870505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEO6979684.1"
                     /translation="MSKKDKKIEIQILDAQVTVNGTKVDGYQLVIGKKIVGQIAELDS
                     KFAVIKGEQVSGFYKNLDQAMGNIIEEYNLNR"
     gene            complement(4059..5081)
                     /locus_tag="VBC94_001423"
     CDS             complement(4059..5081)
                     /locus_tag="VBC94_001423"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEO6979685.1"
                     /translation="MTVNLDWDNLGFAYRKLPFRYISHFKDGKWDDGKLTDDATLHIS
                     ESSPALHYGQQAFEGLKAYRTKDGSIQLFRPDQNAERLQRTADRLLMPHVPTDKFIAA
                     VKSVVRANEEFVPPYGTGATLYIRPLLIGVGDIIGVKPAEEYIFTVFAMPVGSYFKGG
                     LTPTNFIVSKEYDRAAPNGTGAAKVGGNYAASLLPGKYAHEKQFSDVIYLDPATHTKI
                     EEVGAANFFGITKDNQFITPLSPSILPSITKYSLLYLAKERFGMEAIEGDVFVDELDK
                     FTEAGACGTAAVISPIGGIQNGDDFHVFYSETEVGPATRKLYDELVGIQFGDVEAPEG
                     WIYKVD"
     gene            complement(5195..7654)
                     /gene="parC"
                     /locus_tag="VBC94_001424"
     CDS             complement(5195..7654)
                     /gene="parC"
                     /locus_tag="VBC94_001424"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEO6979686.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGFRKSAKSVGNVMGNFHPHGDSSIYDAMVRMSQDWKNRETLIEMH
                     GNNGSMDGDPAAAMRYTEARLSEIADYLLQDIDKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGATGISAGYATDIPPHNLAEVIDAVVYMIDHPKAKLDKLMEFLPGPDFPTGAI
                     IQGKDEIRKAYETGKGRVAVRSRTAIETLKGGKKQIIVTEIPYEVNKSVLVKRIDDVR
                     VNNKVPGIAEVRDESDRDGLRIAIELKKEADETIVLNYLFKYTDLQVNYNFNMVAIDD
                     YTPKQVGLSRILTSYIAHRREIIIARSKFDKEKAEKRLHIVEGLIRVLSILDEVIALI
                     RASENKADAKENLKVSYEFSEAQAEAIVTLQLYRLTNTDIVTLREEEEELRQQITMLK
                     AIISDERTMYNVMKRELREVKKKFANTRRSELQELAETIEIDTASLIIEEDTYVSVTR
                     GGYVKRTSPRSFNASTVDELGKREDDELIFVSNAKTTQHLLMFTNLGNLAYRPVHELA
                     DIRWKDVGEHLSQNLVNFASNEEIIYAELVDDFTKETYFAVTSLGQIKRFERQEISPW
                     RTYKSKTAKYAKLKNVDDYVVTVAPIQLEDVILVTYNGYVLRFSINDVPVVGSKAAGV
                     KAMNLKDSDHIVSAFIANTTSLYLLTHRGSLKRMAIDVIPTTSRANRGLQVLRELKSK
                     PHRVFKAGPVYLEDSSFEFDLFSSVSNHEGDTFVLEIMSKTGKVYDVDLSQWSFSERT
                     SNGSFVSDKISDEEVFSVKIK"
     gene            complement(7788..9737)
                     /gene="parE"
                     /locus_tag="VBC94_001425"
     CDS             complement(7788..9737)
                     /gene="parE"
                     /locus_tag="VBC94_001425"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEO6979687.1"
                     /translation="MTKQDITVTNYGDDAIQVLEGLDAVRKRPGMYIGSTDGTGLHHL
                     VWEIVDNAVDEALSGFGNRIDVIINKDGSITVTDHGRGMPTGMHAMGKPTVEVIFTVL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEIIRDGAIYRQRFENGGKPVTTLK
                     KIGTAPKSKSGTSVSFMPDQSVFSTIDFKFNTIAERLKESAFLLKNVTLTLTDNRSEE
                     AEHLEFHYENGVQDFVEYLNEDKETLTPIMFFEGEEQEFHIEVALQYNDGFSDNILSF
                     VNNVRTKDGGTHETGLKSAITKSMNDYARKTGLLKEKDKNLEGSDYREGLSAILSILV
                     PEEHLQFEGQTKDKLGSPLARPIVDGIVSEKLTYFLMENGDLASNLIRKAIKARDARE
                     AARKARDESRNGKKSKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTAKAKMADIIKNEEINTMIHTIGAGVGPDFNLDDINYDKVIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEEGHVYIALPPLYKMSKGKGKKEIVEYAWTDIEL
                     EELRQKFGKGSLLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLM
                     GDKVPPRRQWIEDNVKFTLEENTVF"
     gene            9851..10489
                     /gene="plsY"
                     /locus_tag="VBC94_001426"
     CDS             9851..10489
                     /gene="plsY"
                     /locus_tag="VBC94_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEO6979688.1"
                     /translation="MNIIIMIIIAYLLGSIQTGLWIGKYFYQVNLRQHGSGNTGTTNT
                     FRILGVKAGIVTLTIDILKGTLATLIPIILGVTTVSPFFIGFFAIIGHTFPIFAQFKG
                     GKAVATSAGVLLGFAPSFFLYLLVIFLLTLYLFSMISLSSITVAVVGILSVLIFPLVG
                     FILTDYDWIFTTVVILMALTIIIRHQDNIKRIRKRQENLVPFGLNLSKQKNK"
     gene            complement(10555..>11170)
                     /locus_tag="VBC94_001427"
     CDS             complement(10555..>11170)
                     /locus_tag="VBC94_001427"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEO6979689.1"
                     /translation="LPNHYYNKINTFMDAVYESGIVYPPRDKVFNAIQITPLENVKVV
                     IIGQDPYHGPQQAQGLSFSVPDNLPAPPSLQNILKELAEDIGSRSHHDLTSWAQQGVL
                     LLNACLTVPEHQANGHAGLIWEPFTDAVIKVVNQKETPVVFILWGGYARKKKSLIDNP
                     IHHIIESPHPSPLSAYRGFFGSRPFSRTNHFLEEEGINEIDWLN"
BASE COUNT         3456 a         2232 c         1796 g         3686 t
ORIGIN      
        1 gtagactata atggaaggag tttttcatga aattatacgt tcaattaatg gttattttaa
       61 ccttttcatt cgctggtgag gttctatcta ctatattcaa cttacctata ccaggtagta
      121 ttattggatt aatattacta ttcctagcgt taaaatacaa aatcatcagg ctcaggcata
      181 ttgatactgt ggggaatttt ctattagcaa atatgaccat tttattttta ccaccagcag
      241 ttggactgat ggagcacttt caagacataa agccctatct ttttgggata gctatcatta
      301 tcttaggagc gctatttctc aatattctaa ctattggact ggtatcccaa tggatcaaaa
      361 agagatatga aggagattat ccagaaattg gaggtaaaaa tggcaacttt aacgaataac
      421 cctatctttg gtattatgtt aaccgtatgg gcgtattaca ttggcataag gatttttaga
      481 aaatatcctt cccctgcaac aactccactt cttttggcga ctattttatt aattgctttt
      541 ttaaaattaa cacatatctc ctataaagat tactacaatg gtggttcatt tctaacgatg
      601 ttaattacgc cttcaacggt tgtattggct attcctctct atagaacctt tcatttaatg
      661 aagcatcaca ttaaaagtat tagcattagc atcatcttag cttctgttat caacacagtt
      721 tttaccgcaa tagttgctaa gttttttggt atgaaatatt tcttagcaat ctcacttttc
      781 ccaaagtccg taactacagc tatggccgtt ggaattacaa gtaaagcagg tggattagct
      841 actatcactc tggtcgtagt tgttattaca ggcatcttaa caagcgtttt ggggcctatt
      901 tttctaaaat tattaaaaat agaagatcct gttgctatag ggctcgcgct aggaggaact
      961 gggcatgcta taggaactgg acaagcctta aaatatggcc aagttcaagg cgccatggct
     1021 ggactagcaa ttggtatcac aggaatttgc tatgttattg tcagtccttt agttgcaggc
     1081 ttaatattga aatgaggaaa agaaaagtaa acccaaaata attatgttat aatatttaaa
     1141 aatagaattt aggataaata attatgatac cgttaaaaaa aataactgtt ggaacgattg
     1201 tctgtctctc atttttgggt ttaacagcat gctcttcttc aaatactcag caaacctcta
     1261 cttcaaaatc aaatgtatca caacataaaa atatcaaagc tgaccacgaa gaattacgtt
     1321 tgaaatttaa taaagtaaag ttaggggtta aagcaaataa ctttaaagga ggcactagtc
     1381 tcgctgaatt aaaacaatta tttggtggcg aaccaaatga aaaatttgat acaccagcgg
     1441 gaaatgtcac tcttaaaggc tatcgttgga atgtcgatga tatctccatc actatacaat
     1501 tattaaacga tagtagcatt gttcgttcaa ttagtaattt taagtttatt cgtgatgcca
     1561 acatcacaac aaaagattat aatagtttaa aaaatggcat gtcatacaat aaagtaaaag
     1621 aacttctagg agaacctgat gatatttctc aagcagtttc ttcagataaa gaggaactac
     1681 aggctgcatg gattagtggc atccagtcgt cagacagtga cccgggaatt aaccttacat
     1741 ttgaaaatga taaactcact aacaaacaac agcatggttt aaaataaaaa ggcattaacg
     1801 cctttttatt aataccttaa ttacttatta gaaacatttt attacacaat ggtttaatct
     1861 tagtacctta acttagcccc ttaatggtca tactaactaa ttatgatact aacttacaca
     1921 gatttcaaac tgattctccc ttatttgtca accctagttt tacttatcag aaatgaggag
     1981 aatcagtctg tgaggcaagg ttggagataa actaatataa agcctataac ataaagcttt
     2041 atggagcatc tattatccat cttaagggaa tcgaacccct atcccaagaa ccggaatctt
     2101 gtgtgatatc cattacacta aagatggtag tacatgttac attatagcat actttatcaa
     2161 caaaaacaaa aagacttgct ttagcaagtc ttttaattat tataattcaa tatcaccgaa
     2221 taagtcagcc atagagaatc cagtttgtgt ttctggaagc tcatagtcgc gtttttcttg
     2281 acgacgtgga cgacgtggac gtgattgacg tttttcttct ttttcacctt ctgcttgcgc
     2341 tggacgctct tcaagagctt tcattgaaag tgatacacgt tctgcatctg aattaacttc
     2401 aagaacttta acagtaactt cttgtcctgc agataatacg tctttaggat tttcaacacg
     2461 tttgtgtgaa atttgtgaaa tgtgtacaag tccatcaatg ccaggtaaca cttcaacaaa
     2521 agcaccaaag tcagtcaagc gttttacttt accttcaata acatcaccag cagcaagttt
     2581 ttgttcaacg ccatcccatg ggccaggtgt agttgcttta agtgaaagtg atacacgacc
     2641 agcttcttca tcaattgaaa gtactttaac ttcaacttct tcaccaacag ttacaactga
     2701 tttaggtgaa acattgcgct catgtgataa ttcagttacg tgtacaagtc catcaacacc
     2761 accaaggtca atgaaagcac caaagcttgt taaacgagca actttaccag ttacaactga
     2821 acctacttca atgtttgaga aaacttcttt acgtgcagca gctgctgatt cttcaacaac
     2881 ttcacgacgt gaaaggatga agcgattttc tgctgcatca acttctttga ttttagcatc
     2941 aaattcttgt ccaacaaatt tttcagtgtt acgtacaaaa cgagtatcaa tcattgaagc
     3001 tgggatgaaa ccacgaagtc cttcaaattc aactgaaagt ccgcctttaa cagcacgtgt
     3061 acctttaaca gtaacaactt caccttcacg accaacaagt ttgtcccatg ctttacgagc
     3121 ttccaagcgt tttttagata caaggaaagt tactgtatca gtatctttac ctacaacttg
     3181 acgaagaaca agtacttcaa ctgtgtcgcc agctttaaca aaatcattga tatcagcatc
     3241 acgatcgtta gtcaattcac gaagtgtcaa gacaccttca acaccagtac catcgataac
     3301 aacgtttgct tgaccgttat caactgttaa aacttccgca gtgacaacgt caccaggatt
     3361 cacttcgcta acactgttta gcaaatcttc aaattcattc attctaaaaa atcctccaac
     3421 aaaagctagc cgagctagct tttgataaca atatattttc tcaaggtaac ccgcaaggac
     3481 aacaaatatc tctttgacgg tgtcaaacaa aagtttgata cctttgactg gggtagctgg
     3541 attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa
     3601 ataatggaaa gagagggatt cgaacccccg aacccgaagg agcggattta cagtccgcca
     3661 cgtttagcct cttcgctatc tttccgtaac aacactgact attctaccac agatgttgtt
     3721 agcacgcaag ctttttaaat aaatattagc gattaagatt atattcttca ataatatttc
     3781 ccatagcttg atctaaattt ttatagaatc cactcacttg ctcaccttta atcacagcaa
     3841 atttagaatc tagctctgca atttgaccta caattttttt tccaataacg agttggtatc
     3901 catcaacctt agttccatta actgttactt gagcgtctaa aatttgaatt tcaattttct
     3961 tatctttttt actcactatt aactccaata tctcttttat tgttttttat tgtacacaaa
     4021 atactaaaag cttgcaagag caagctttta gtatagactt aatctacttt ataaatccac
     4081 ccttctggtg cttcaacatc accaaactgg atccctacta actcatcata tagtttacgt
     4141 gtcgctggac caacttctgt ctcactgtag aagacatgga agtcatctcc gttttgaatt
     4201 cctccaattg gtgaaataac tgcggctgta ccacatgcgc cagcttctgt gaatttatct
     4261 aattcgtcga caaagacatc tccttcaatc gcttccatac caaacctttc tttagcaagg
     4321 taaagcaatg aatatttagt aattgaaggt aaaattgaag gactcaaagg ggtgataaac
     4381 tgattatctt tagtaattcc aaagaaatta gctgctccaa cctcttcaat tttagtatgt
     4441 gtcgctggat caagatagat aacatctgaa aattgtttct catgagcgta tttcccagga
     4501 agaagactag cagcatagtt accaccaact tttgcagctc ctgtaccatt cggtgctgct
     4561 ctatcatatt ctttggaaac aataaagtta gttggtgtta aaccaccttt aaaataacta
     4621 cctactggca ttgcaaatac cgtaaaaata tattcttcag caggtttaac tccaataata
     4681 tctcccacac caattaataa gggacgaatg tataatgttg cccctgtacc atacggtgga
     4741 acaaactcct cattagcgcg tacaacagat ttaaccgctg caataaattt gtctgtcgga
     4801 acatgtggca ttaaaagacg atctgctgtt ctttgaagtc gttctgcatt ttgatctgga
     4861 cgaaataact gaatagagcc atctttagtt ctataagctt tcaatccttc aaaagcttgt
     4921 tgcccatagt ggagggcagg agaactttct gaaatatgta gcgttgcatc atctgtcaac
     4981 ttgccatcat cccatttccc atctttaaaa tgcgaaatgt atcggaatgg taattttctg
     5041 taagcaaaac ctaaattatc ccaatctaaa tttactgtca taaggtacct ctttctttgg
     5101 gtttctaact attgtacgct attatcatta ttttttcaat attatttgta aaattcagat
     5161 aaattaccaa actagtaaaa acacaaatat gtttttactt aattttaact gagaatactt
     5221 cttcgtcaga aatcttatca gatacaaaag agccattact tgtacgttca gaaaaactcc
     5281 attgacttaa atcaacatcg taaacttttc ctgttttaga cataatctca agtacgaaag
     5341 tatctccttc atgattcgat actgaactaa ataaatcaaa ttcaaaagat gaatcctcta
     5401 aatatacagg tcctgcttta aaaacgcgat gcggtttgga tttcaattca cgaagaactt
     5461 gaagtcctcg attagcacga ctagttgtgg ggataacatc aatggccatt cgtttaagac
     5521 ttcctctatg agttaataaa tataaacttg tggtgttagc tataaatgca gatacgatgt
     5581 gatcactatc ttttaaattc atcgctttta cacctgcggc tttactacca acgactggta
     5641 catcatttat actaaatctc aacacataac cattataagt gacaagtata acatcctcta
     5701 attggatagg agcaacagtc acaacgtaat catctacatt ttttaactta gcatatttag
     5761 ctgtttttga cttatatgtc cgccatggac tgatttcttg acgttcaaaa cgctttattt
     5821 gccctaaact agtgacagca aaataagttt ctttagtaaa atcatctact aattcagcat
     5881 aaataatttc ttcatttgaa gcaaagttaa caagattttg tgataaatgt tcaccaacgt
     5941 ctttccatcg aatatcagct aattcatgaa ctggacgata agcaagatta ccaaggttag
     6001 taaacatcaa taaatgttgg gtagtctttg cattgctcac aaatatcaat tcgtcatctt
     6061 ctcgcttacc aagttcatca acggttgatg cattgaaaga gcgagggctg gtgcgtttta
     6121 catatccgcc tctagtaaca ctgacgtatg tgtcctcttc gataattaaa cttgcagtat
     6181 cgatttcaat agtttcagct aattcttgaa gttcagaacg tctagtattg gcaaatttct
     6241 ttttgacctc acgtaactca cgtttcatta cgttatacat cgtacgttca tcactaataa
     6301 ttgccttgag cattgtgatt tgttgacgaa gttcttcttc ttcttctctc aacgtcacaa
     6361 tatcagtatt tgtgagacga tataattgca atgtgacgat agcctctgct tgagcttctg
     6421 aaaactcata gctgaccttc aaattctctt tcgcatcagc tttattctca gaagcacgaa
     6481 ttaatgcaat cacttcatcc aaaatagaaa gaaccctaat cagtccctca acaatatgga
     6541 gacgtttttc ggctttttct ttatcaaatt ttgaacgagc aataataatt tctcttcggt
     6601 gagcgatata gctagttaat attcttgata gacccacctg cttaggggta tagtcatcaa
     6661 tagcaaccat attgaaatta taattaactt gtaggtctgt atatttaaaa agataattta
     6721 agacaattgt ttcatcggct tcttttttga gctcaatagc gatacgaagg ccatctctat
     6781 cagactcatc acgcacttct gctattcctg gaactttatt attaacacga acatcatcga
     6841 ttcgttttac taaaactgac ttattaactt cataaggaat ttcagtaaca ataatttgtt
     6901 tcttaccacc ttttaaggtt tcaatagcag ttcgcgagcg aaccgctact ctccccttac
     6961 cagtctcata tgccttacga atttcatctt ttccttgaat gatagcgcca gttggaaaat
     7021 ctggaccagg tagaaattcc attaatttat ctaatttagc tttagggtga tcaatcatgt
     7081 acacgacagc atcaatgact tctgctaaat tatgtggtgg aatatctgtc gcatagcctg
     7141 cagatattcc tgttgctccg ttgactaaaa gattaggaaa ggcagctggt aaaacagttg
     7201 gttctttctc tgtatcatca aaattccacg caaagggtac agtattctta tcaatatctt
     7261 gaagaagata atcagcaatt tctgatagac gggcttccgt ataacgcata gctgccgcag
     7321 gatctccatc catagaacca ttatttccgt gcatttcaat caatgtctca cgatttttcc
     7381 aatcttgaga catacgaacc atcgcgtcat agatagagga atccccatga ggatggaaat
     7441 tccccataac attaccgact gattttgcag atttgcgaaa tcccttttca aaagtattac
     7501 catctttatt cattgaataa aggattcgac gttgaacagg ttttaagcca tcacgaatat
     7561 ccggcaacgc ccgttcttga atgatatact tagaatagcg cccaaaacgc tctcccataa
     7621 tgtcctcaag ggacatattt tgaatattag acatattttt aattttctcc tagttggagt
     7681 ggcttagtat tatttttcca cttatttgaa atgaatgatt ggctctcttg tctaacgcat
     7741 acaatattac ttgtacacta taaaacatga ataaccaagg ctctttatta aaatactgta
     7801 ttctcttcta aagtaaactt gacattatct tcaatccatt ggcgtcttgg aggaacttta
     7861 tcacccatca agacattaac tcgacgttct gctcttgcta aatcttcaat tgttacacga
     7921 attaatgtcc ttgtctcagg gttcatagtg gtttcccaca attgatcggc attcatttct
     7981 ccaagccctt tatatcgttg taatagtgag cctttaccaa atttttgtcg taattcttct
     8041 aactctatat ccgtccaagc atactcaaca atttctttct taccttttcc ttttgacatt
     8101 ttatacaaag gtggcaaagc aatatagaca tgtccctctt caacaagagg tctcatatag
     8161 cgataaaaga aagttaataa tagtgtctga atatgagctc cgtcagtatc agcatcggtc
     8221 ataatgataa ctttatcata attaatatcg tcaagattaa aatcaggacc aacacctgca
     8281 ccaatggtat ggatcattgt attaatttct tcgtttttta taatatcagc catctttgcc
     8341 ttagctgtat tcaatacttt tcctcgcaac ggtaaaatag cttgaaactt tctatcacga
     8401 ccttgcttag cagagccacc tgcagagtct ccttcgacta aatagagttc atttttctta
     8461 gcattttttg attgtgcagg ggttaatttt ccagaaagta gacctttatc ttttttcgac
     8521 tttttcccat tcctactttc atcacgtgct ttacgtgcag cttcacgcgc atcacgtgct
     8581 tttattgcct tacggattaa attagatgct aaatctccat tttccataag aaagtaagtt
     8641 aacttttcag aaacaatacc atcaacaatt ggtcgtgcta agggacttcc tagtttatct
     8701 ttagtttgac cttcaaattg caagtgctcc tcaggtacaa gaatagataa aatggctgat
     8761 agcccctcac gataatcaga accttcaagg tttttatctt tttctttaag aagtcctgtt
     8821 ttacgtgcat agtcattcat agactttgta atagctgatt tcaagccagt ttcatgtgtt
     8881 ccaccatctt tagtacgaac attattgaca aaagaaagga tattatctga aaaaccatca
     8941 ttatactgca aagcaacttc gatatgaaat tcttgttctt caccttcgaa aaacatgatt
     9001 ggtgtcaacg tttctttatc ttcatttaaa tactctacaa aatcttgaac accgttttca
     9061 taatgaaatt ctagatgctc tgcttcttct gaacgattat ctgttaaggt taaagtaaca
     9121 ttttttaaga gaaaggcaga ttcctttaag cgctctgcaa ttgtattaaa cttaaaatct
     9181 atcgttgaaa atactgattg atctggcata aagctgacag aagttccaga ttttgatttt
     9241 ggtgccgtgc caattttttt cagagttgta acgggcttac caccattttc aaaacgttgt
     9301 ctgtaaatag ctccgtcgcg aatgatttca acttctaacc aagaagacaa agcattaact
     9361 acagatgatc caacaccatg gagaccacct gatgttttat aaccaccttg tccaaatttt
     9421 ccacctgcat gaagcaccgt gaagatgact tcaacagtag gttttcccat agcatgcatt
     9481 ccagttggca taccacgtcc atggtctgta acagttatac tcccatcttt gttaataatc
     9541 acatcaatgc gatttccaaa acctgataga gcttcatcaa ctgcattatc tacaatttcc
     9601 cacactaaat gatgtaaacc agttccatct gtggaaccga tatacatccc tggacgctta
     9661 cgaacagcat ctaatccttc taagacttga atagcatcgt cgccataatt cgtcactgtt
     9721 atatcttgtt tagtcaaagt gacctccaaa ttttttcatc ttttttatat tacaagtttt
     9781 ttgctgtttt tgcaaaatta tttttttaaa aatagcaaaa agacttttat gcttatgtta
     9841 taataagggt atgaatatta taattatgat tatcattgcc tatctgttag gctctatcca
     9901 aactgggctt tggattggta aatattttta ccaagtcaat cttcgtcaac acggtagtgg
     9961 taatactggc accacgaaca ccttccgtat tttaggtgta aaagctggca ttgtcacttt
    10021 gactattgac attttaaaag ggactctagc aacacttatt cctatcatat taggtgtaac
    10081 gacagtatct ccatttttta tcggtttttt tgctattata ggtcacactt tccctatttt
    10141 tgcacaattc aaaggtggga aagccgttgc tacaagcgct ggggttttgc taggatttgc
    10201 accttctttt ttcctttact tattagtgat ctttttatta acgctttatc tttttagcat
    10261 gatttcgcta tccagtatta cagtagctgt cgtaggtatc cttagtgtct taatttttcc
    10321 attagtaggt tttatattaa cggactatga ctggatattt accactgtgg ttatcttaat
    10381 ggccctcacg attattatcc gacatcagga taatatcaaa cgtattcgaa aaaggcaaga
    10441 aaatttggtt ccgtttggct taaacctaag taaacaaaaa aataaataat aaaaaaccgc
    10501 tactagcggt tttttattat aagactaaca atgtttcgag agtagatttc ccttctaatt
    10561 taaccaatca atctcattta ttccttcttc ttctaagaaa tgatttgttc tagaaaaagg
    10621 tctgctacca aagaaaccac gataagcaga taggggactc ggatgaggac tctcaataat
    10681 atgatgaata ggattatcaa ttagcgactt tttctttcga gcatagccac cccacagaat
    10741 aaagactact ggggtttcct tttggttaac cactttgatg acagcatcag taaaaggttc
    10801 ccatataagc ccagcatgac cattagcttg gtgttctgga actgttaaac aagcattcaa
    10861 tagcagcact ccttgttgcg cccatgatgt taagtcatga tgtgaacgtg acccaatatc
    10921 ttctgctaat tcttttagaa tattttgaag tgaaggagga gctggtaaat tgtctggaac
    10981 agaaaatgag agtccttgag cttgttgagg gccatggtaa ggatcttggc caataatcac
    11041 tacttttacg ttttctagtg gtgttatctg tatggcattg aacaccttat ctcttggagg
    11101 ataaacgatt ccgctttcgt aaactgcatc cataaatgta ttaattttat tgtagtaatg
    11161 attgggtagt
//
LOCUS       DATROQ010000060        11118 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.061, whole genome shotgun sequence.
ACCESSION   DATROQ010000060 DATROQ010000000
VERSION     DATROQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11118
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.061"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(13..1092)
                     /locus_tag="VBC94_001428"
     CDS             complement(13..1092)
                     /locus_tag="VBC94_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6979690.1"
                     /translation="MQKMTKLRRFRFPLRFYFTLMFVLTMLFSVLASLLLVAAIVFTF
                     FQGVLTTHVLQVSALAVVFLSLVIASISMWYGSYHLTKPILDISHIVSNVAGGDFEGH
                     IYRNSNRRKSYEYYNELDELSESINQMIVSLSHMDHMRKDFITNVSHELKTPIAAVAN
                     IVELLQDPELDEETQSELLGLVKTESLRLTRLCDTMLQMSRVDNQETIGELSSVRVDE
                     QIRQAMISLTERWQAKRINFQLDSKPYTVYSNSDLLMQVWINLLDNAIKYSEDIVDLS
                     VRMEETNNHYLRVIISDKGRGISQYDVQHIFDKFYQADQSHNQQGNGLGLAIVKRIIV
                     LCKGHISVSSQLEIGTEFCVELPLS"
     gene            complement(1076..1747)
                     /locus_tag="VBC94_001429"
     CDS             complement(1076..1747)
                     /locus_tag="VBC94_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6979691.1"
                     /translation="MIKILVVEDNIVQQKIITTKLTQEGYQFITASNGQEALNCLDTE
                     EVQLIITDIMMPMMDGYQLIQELRSAAYNVPIIVMTAKSQMEDMTKGFGLGADDYMVK
                     PVQLQELALRIKALLRRANIVAQHQLIIGNTCLNEDELSLKYFEQEIVFPQKEFRVLF
                     HLLSYPNRIFTRLELLDSIWGMDTDLDERVVDACINKIRRKVEHLPDFKIETVRGVGY
                     RAKND"
     gene            complement(1778..2464)
                     /locus_tag="VBC94_001430"
     CDS             complement(1778..2464)
                     /locus_tag="VBC94_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979692.1"
                     /translation="MSAILELKHISKHYPDGDELLSILDNLDLSVSAGEFVAILGPSG
                     SGKSTLLSIAGLLLGADQGSLYVNHENVTDLSQRQRTQLRREALGFIFQSHQLLPYLT
                     IQEQLQQEARFAKHYDKKTSLEEINKLLSDLGIEQCAHKYPNQLSGGQKQRAAIARAF
                     INHPKVILADEPTASLDEERGRQVTELIRQEVKSHNTAAIMVTHDERVLDLVDTVYRL
                     KDGKLVKENF"
     gene            complement(2464..3552)
                     /locus_tag="VBC94_001431"
     CDS             complement(2464..3552)
                     /locus_tag="VBC94_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6979693.1"
                     /translation="MSHAWKELTFYKKKYLLIELLIIVMMFMVVFLSGLANGLGRAVS
                     AAIENNPAQTYILNEGAEQVITSSVLTTKDQTDLNSLNLKDSTTLNIQRSSLTRQGHE
                     KKIDISYFAIDKDSFMAPTLSEGKQLTSYKKAIILNDSLKAEGIKLGDKVIDKSSSIS
                     LTVVGFVHNSMYGHGPVAFIDKDIYTEINKKINPQYQFLPQALVMKNDKSISHLPTQL
                     EAVSKKDVIQHIPGYSAEQSTLNMILWVLVVASAGILGVFFYIITLQKRHEFSVMKAI
                     GTKMSEIALFQLSQVIILALFGIIVGDRLAIALSYVLPAQMPFVINWQNIILVSFVFL
                     VIAMISSALSIVKVAKIDPVEVINGGGE"
     gene            3734..4720
                     /locus_tag="VBC94_001432"
     CDS             3734..4720
                     /locus_tag="VBC94_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6979694.1"
                     /translation="MSTIRQVAEKAGVSTSTVSRYISQNGYVSQKASQKIEQAIRELH
                     YVPNFLAQSLKTKKNQLVGLLLPDISNPFFPRLARGVEEFLKEQGYRVMLGNTNNKSH
                     LEEEYLNVLLQSNAAGIITTHDFTKNHPEIDIPVVVVDRVNQETQYGVFSDNKEGGKL
                     AAQAIWTAGATNILLIRGPLDKADNLNQRFQGSQNYLLNKGARFAIEDSASFDFAEIQ
                     IEAKTLLDHHPDIDSIIAPSDIHAIAYLHEILNRGKRIPEDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYIMGQKAAELIFKITNQLPITNKRIKLPVHYVERETLRRKIDE"
     gene            4713..5624
                     /gene="rbsK"
                     /locus_tag="VBC94_001433"
     CDS             4713..5624
                     /gene="rbsK"
                     /locus_tag="VBC94_001433"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEO6979695.1"
                     /translation="MSNIVIIGSISMDLVMETNRIAKEGETVFGQRFSMVPGGKGANQ
                     AVAIGRLSQDRDNITILGAIGEDSFGPILLDNLNKNHVTTDFVGTIPSSSGVAQITLY
                     NNDNRIIYCPGANGKVDTKKWSQEWSIIKEADLVVLQNEIPHQANMKIANFCKEHSIK
                     LLYNPAPSRKTDIEMLDKVDYFTPNEHECQELFPNQKLEDILATYPEKLIVTLGTKGA
                     IYSDGKESHLIPALETKAVDTTGAGDTFNGAFGYAISKKFKIANALRFATLAAHLSVQ
                     KFGAQGGMPTIKEMEDNQHYEKDWYIK"
     gene            5599..5997
                     /gene="rbsD"
                     /locus_tag="VBC94_001434"
     CDS             5599..5997
                     /gene="rbsD"
                     /locus_tag="VBC94_001434"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEO6979696.1"
                     /translation="MKKTGILNSHLAKLADDLGHTDQVCIGDLGLPVPNGIPKIDLSL
                     TSGIPSFQEVLDIYLENILVEKVILAEEIKEANSDQLSRLLAKLDNSVNIEYVSHNHL
                     KQMTQDVKAVIRTGENTPYSNIILQSGVII"
     gene            6013..7491
                     /locus_tag="VBC94_001435"
     CDS             6013..7491
                     /locus_tag="VBC94_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEO6979697.1"
                     /translation="MKIDMRNISKSFGTNKVLEKIDLELQSGQIHALMGENGAGKSTL
                     MNILTGLFPASTGTIYIDGEERTFSNPQEAEEFGISFIHQEMNTWPEMTVLENLFLGR
                     EIKTTFGLLNQKLMRQKALEAFKRLGVTIPLDIPIGNLSVGQQQMIEIAKSLLNQLSI
                     LVMDEPTAALTDRETENLFRVIRGLKQEGVGVVYISHRMEEIFKITDFVTVMRDGVIV
                     DTKETSLTNSDELVKKMVGRKLEDYYPEKHSEIGPVAFEVSNLCGDNFEDVSFYVRKG
                     EILGFSGLMGAGRTEVMRTIFGIDKKKSGKVKIDNQEITITSPSQAIKQGIGFLTENR
                     KDEGLILDFNIKDNMTLPSTKDFSKHGFFDEKTSTTFVQQLINRLYIKSGRPDLEVGN
                     LSGGNQQKVVLAKWIGIAPKVLILDEPTRGVDVGAKREIYQLMNELADRGVPIVMVSS
                     DLPEILGVSDRIMVMHEGRISGELSRKEADQEKVMQLATGGK"
     gene            7493..8434
                     /gene="rbsC"
                     /locus_tag="VBC94_001436"
     CDS             7493..8434
                     /gene="rbsC"
                     /locus_tag="VBC94_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771495.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HEO6979698.1"
                     /translation="MKNMMKYISELTTLIALAGLMVIITLINPNFLTTNNLLNLLLQV
                     TANGFIAFGMTFVILTGGIDLSVGSILALSSALTAGLIGHGVPVGIAIVLSVALGGIL
                     GMLNGLFISYGKLAPFIVTLATMTIFRGATLVYSNGNPITAGLSDSFLFQFLGQGYIV
                     GIPFPVILMFLTFIILYILLHKTAFGKSVYALGGNEKAAYISGIKLNKVKIIIYTISG
                     IMASISGLIITSRLSSAQPTAGASYEMDAIAAVVLGGTSLSGGKGRIIGTLIGALIIG
                     VLNNGLNIIGVSAFWQQVVKGIVILMAVLLDRFKVAK"
     gene            8487..9455
                     /locus_tag="VBC94_001437"
     CDS             8487..9455
                     /locus_tag="VBC94_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEO6979699.1"
                     /translation="MKFGKKLGFLALLMSIVLILGACGKTGLGNSSGNSTKNVTKKSA
                     KDLKLGVSISTTNNPYFVAMKDGIDKYASNKKISIKVADAQDDAARQADDVQNFISQN
                     VDAILINPVDSKAIVTAIKSANNANIPVILMDRGSEGGKVLTTVASDNVAAGKMAADY
                     AVKKLGKKAKAFELSGVPGASATVDRGKGFHSVAKSKLDILSSQSANFDRAKALNTTQ
                     NMIQGHKDVQIIFAQNDEMALGAAQAVKSAGLQNVLIVGIDGQPDAHDAIKKGDISAT
                     IAQQPAKMGEIAIQAAIDHYKGKKVEKETISPIYLVTKDNVEKYNW"
     gene            complement(9495..10949)
                     /locus_tag="VBC94_001438"
     CDS             complement(9495..10949)
                     /locus_tag="VBC94_001438"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEO6979700.1"
                     /translation="MANKIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGDFVIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPETHENYRQSERLELYQRYIDQLLAEGKA
                     YKSYVTEEELAAERERQELAGETPRYINEFIGMSETEKEAYIAEREAAGIIPTVRLAV
                     NESGIYKWTDMVKGDIEFEGSNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPQFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     SEAVFNFIALLGWNPGGEEEIFSREQLINLFDENRLSKSPAAFDQKKMDWMSNDYLKN
                     ADFESVFALCKPFLEEAGRLTDKAEKLVELYKPQLKSADEIVPLTDLFFADFPELTEA
                     EKEVMAAETVPTVLSAFKEKLVSLSDEEFTRDTIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIYLLGKEKSVQHIDNMLAKL"
BASE COUNT         3586 a         2082 c         1938 g         3512 t
ORIGIN      
        1 tgttttaaat aattatgata aaggtagttc tacacaaaat tctgttccaa tttccaactg
       61 actggagaca ctaatatgtc ccttacaaag aacaattata cgtttaacta ttgctagtcc
      121 taatccattt ccttgttgat tatgcgactg atccgcttgg taaaatttat caaatatatg
      181 ctgaacatca tattgagaaa ttcccctacc tttatcagaa ataataactc tcagataatg
      241 attattagtt tcttccattc gtacacttaa gtctacaata tcctcggaat attttattgc
      301 attatctaaa aggtttatcc aaacttgcat cagcaagtca gagtttgagt aaacagtata
      361 tggttttgaa tccagttgaa aatttatcct tttagcttgc caacgttctg ttaaactaat
      421 cattgcttga cgaatttgtt catctacacg aacagaggat agctccccaa tagtctcctg
      481 attatccacg cgcgacattt gcaacatcgt atcacataat cttgttaacc ttaggctctc
      541 tgttttaaca agacctaata actcactttg tgtttcttca tctaattctg gatcttgtaa
      601 tagttctaca atattagcta ctgcagcaat cggagtcttt agctcatgag aaacattagt
      661 gataaagtct tttcgcatat gatccatatg agataaagaa acaatcatct gattaatact
      721 ttcagatagc tcatccaatt cattataata ctcataggat ttacgacgat tactgtttcg
      781 ataaatatgc ccttcaaagt caccgcctgc aacattggac acaatatggc taatatctaa
      841 aattggtttt gttaaatgat aagaaccgta ccacatacta atactagcta tcacaaggct
      901 taaaaaaaca acagcgagag cgctaacttg taatacatga gtagttaaga ctccttgaaa
      961 aaaagtaaaa actattgctg caactagtaa aagagaagct aggactgaga aaagcatcgt
     1021 taacacaaac attaatgtga aataaaatct taacggaaac cgaaatcgcc ttagcttagt
     1081 cattttttgc acgataccct acccctctca ccgtttcaat cttaaaatct ggtaagtgtt
     1141 ctacttttcg tctaatttta ttgatgcaag catctaccac ccgctcatct aaatcagtat
     1201 ccattcccca aatactatcc aatagttcta aacgtgtgaa aatcctatta ggatagctaa
     1261 gtaagtggaa aagaacccta aattctttct gaggaaatac aatttcttgc tcaaaatatt
     1321 tcaagcttaa ttcatcttca ttaagacaag tattcccaat aattagctga tgttgagcta
     1381 caatattagc tctcctcaag agtgccttaa tacgtaaagc taattcttgt aactgcacag
     1441 gcttaaccat ataatcatct gcaccaaggc caaacccttt tgtcatatct tccatttgtg
     1501 attttgctgt cataacaatg attgggacat tgtaagcagc tgacctgagt tcttgaatta
     1561 attgataacc atccatcatg ggcatcatga tatcagttat aatcaattgt acttcttctg
     1621 tatctagaca atttagagct tcttgtccat ttgaagcagt gataaattgg tagccctctt
     1681 gtgttaattt tgtggtaatg attttttgtt gaacaatgtt atcctctaca actaatattt
     1741 taatcattaa atcacctcta gcttattgta acacaagtta gaagttttct ttaactaatt
     1801 tcccgtcctt taaacgataa acggtatcta ctaaatctag aacgcgctca tcgtgtgtca
     1861 ccataatagc tgcagtatta tgtgatttaa cttcctgacg aataagttct gtaacttgac
     1921 gcccacgttc ttcatcaagg ctagctgttg gctcatctgc caagataacc ttaggatgat
     1981 taataaatgc tctagcaatt gctgcgcgtt gcttttgtcc tcccgataat tggttagggt
     2041 atttatgtgc acactgctct attcctaaat ctgataataa tttattaatt tcttccaagc
     2101 ttgttttctt atcatagtgt tttgcaaatc gagcctcttg ttgcaactgt tcttggattg
     2161 ttaaataagg aagcagttga tgactttgaa atataaaacc tagtgcttca cggcgcaatt
     2221 gtgttctttg tctttgagac aaatccgtaa cgttctcatg attgacatac aaactacctt
     2281 gatctgcacc aagaagtaac cctgcaatgg ataaaagtgt gcttttacct gaacctgagg
     2341 gtcccagtat agctacaaac tcacctgcag aaactgataa atctaaatta tctaatattg
     2401 ataaaagctc atctccatct ggatagtgtt ttgagatgtg ttttaattct aagattgctg
     2461 acattattct cctcccccat taataacttc gactgggtcg attttagcaa cttttacaat
     2521 tgacaaagca ctactaatca ttgcaatgac taaaaataca aacgatacta gaataatatt
     2581 ttgccaatta ataacaaatg gcatttgagc tggtaataca taagagagag caatcgccaa
     2641 tcgatctcca acaatgatac caaacagtgc caatatgatg acttgactca attgaaatag
     2701 ggcaatctct gacatttttg tcccaatagc tttcatgacg ctaaattcat gacgtttttg
     2761 aagtgtgatg atgtagaaaa agacacctaa aataccagct gaagctacga caaggaccca
     2821 taaaatcata ttcaaagtgg attgttcagc actataacca ggaatatgtt gaatgacatc
     2881 ttttttacta acagcttcta attgagttgg taaatgagat atacttttat catttttcat
     2941 aactaatgcc tgaggtaaga actggtattg aggattaatc tttttattaa tttcggtata
     3001 aatgtcttta tcaataaatg ccacaggtcc gtgaccatac atactattat ggacaaatcc
     3061 aacgactgtt agtgatatag atgatgactt atcaatcact ttatcaccta actttatccc
     3121 ttcagctttt aaactatcat ttaaaataat agcttttttg taacttgtca gttgttttcc
     3181 ctcagaaagt gtaggtgcca taaaagaatc tttatcaata gcaaaataag aaatatcaat
     3241 tttcttttca tgtccttgtc gagttaaact cgaacgttga atatttaaag tggtactatc
     3301 ttttaaattc aaactgttta aatctgtctg atctttagtt gtcaatacag atgatgtaat
     3361 cacttgttca gcaccttcat tcaaaatata ggtttgagct gggttatttt caatagcagc
     3421 actaacagct ctacctaaac cgtttgctaa acctgataaa aaaactacca tgaacatcat
     3481 aacaatgatt agcaattcaa tcaggaggta ttttttctta taaaaggtta attctttcca
     3541 agcatgtgac atggtttttc tcctttgatc ttttgatgtg aacatagtat cacagcttta
     3601 tgacttgaat atgacctcta aaaataatga tattttatca ttaaattatt taaaaaatag
     3661 cttgacaatt ttttagtaag cgattacaat tctaattatg aaaccgttcc cacaataaaa
     3721 gaaaggagtt tctatgagta cgattagaca agtcgccgag aaagctggcg tatcaacatc
     3781 tactgtctca aggtatatat cccaaaatgg ttacgttagt caaaaagcaa gtcaaaaaat
     3841 cgagcaagct atccgcgaac tccattatgt tccaaatttc cttgcacaat ctctgaaaac
     3901 caaaaaaaat caacttgttg gattactact tcccgatatt tcaaaccctt tctttccaag
     3961 acttgctaga ggagttgaag aatttctaaa agaacagggt tatcgtgtca tgcttggcaa
     4021 cacaaataac aagagccatt tagaagaaga gtacttaaat gtgctattac aaagtaatgc
     4081 tgcagggatt attacaacac acgatttcac caaaaatcat cctgaaatag atattccagt
     4141 tgtagttgtt gaccgcgtta atcaagaaac acagtacggc gttttctctg ataataaaga
     4201 gggaggaaaa ttagcagctc aagctatttg gacagcaggt gctaccaata tattactcat
     4261 tcgtggtccg ctagataaag ctgataacct taatcaaagg tttcaaggaa gccaaaatta
     4321 cctattaaac aaaggggctc gttttgctat cgaagatagt gccagttttg attttgcaga
     4381 gattcaaatt gaagcgaaaa ctttacttga ccaccatcca gatattgata gcattatcgc
     4441 tccatctgat atacatgcta ttgcctacct acacgagatt cttaatcgag ggaagcgaat
     4501 ccctgaagat gtacaaatta ttggttatga tgatatttta atgagccaat ttatctaccc
     4561 ttcgttgtca actattcacc aatcatcata tattatggga caaaaagcag ctgaattaat
     4621 tttcaaaatt actaatcaat tacctattac caataagcgt attaaattac ccgttcatta
     4681 tgtcgaacgt gaaaccctaa ggaggaaaat agatgagtaa cattgtcatt atcggtagta
     4741 tttccatgga cttagttatg gaaaccaatc gtatcgccaa agagggagaa accgtatttg
     4801 gccaacgttt ttcaatggtt ccaggaggta aaggtgctaa tcaggctgtc gctattgggc
     4861 gtcttagcca agatcgagat aatattacta ttcttggtgc cattggcgag gattcttttg
     4921 gtcccattct tttggataat ttaaacaaaa accatgttac tacggatttt gtgggaacga
     4981 taccatcttc ttcaggtgtt gcccaaatca ctctttataa taatgataat cgaatcattt
     5041 actgtcctgg agccaacgga aaagttgata ctaaaaagtg gtctcaggag tggtcaataa
     5101 ttaaagaagc tgatttagtt gtattacaaa atgaaattcc tcatcaagca aatatgaaaa
     5161 ttgctaattt ttgtaaagaa catagtatta aattactcta taatcctgca ccatctagaa
     5221 agactgatat tgagatgctc gataaggtcg attatttcac accaaatgaa cacgaatgtc
     5281 aagagctgtt cccaaaccaa aaactagagg atatattagc cacatatcct gaaaaactaa
     5341 ttgtaacact cggcacaaaa ggagccatct actctgatgg aaaagaaagc catttaattc
     5401 ctgccttaga aactaaagct gttgatacaa ctggtgcagg tgatactttt aacggtgcat
     5461 tcggttatgc tatctcgaaa aaatttaaga ttgctaatgc cttacgtttt gcaactctag
     5521 ctgctcactt atccgtgcaa aaatttggtg cacagggtgg catgcctaca attaaagaaa
     5581 tggaggacaa ccaacattat gaaaaagact ggtatattaa atagtcatct cgctaaatta
     5641 gcagatgacc ttgggcatac tgatcaagtt tgtattggag atctgggtct acctgttcca
     5701 aatggcattc caaagattga tttgtctttg acctctggaa tacctagttt ccaagaggta
     5761 cttgatatct atttagagaa tatcttagtt gaaaaagtca ttttagcaga agaaatcaag
     5821 gaagcaaatt cggatcaatt atcaaggtta ttagctaaac ttgataactc tgttaacata
     5881 gagtacgtca gtcacaatca tttaaaacaa atgacccaag atgtcaaagc agttattcgt
     5941 acaggtgaaa atacaccgta ttcaaatatt attctacaat ctggtgttat catctagaaa
     6001 ggagtttagt ccatgaaaat tgacatgagg aatatctcca aatccttcgg tactaataaa
     6061 gttctagaga agattgattt ggaattacaa tctggtcaaa tacacgcact tatgggtgaa
     6121 aatggtgcgg gtaaatcaac cttaatgaac attctaacag gactttttcc agcaagtaca
     6181 ggaactattt atattgatgg cgaggaacga actttttcaa accctcaaga agcagaggaa
     6241 tttggtatta gttttattca ccaagagatg aatacttggc cggaaatgac tgtcttggaa
     6301 aatctctttc ttggacgtga aatcaagaca acttttggct tactcaatca aaaattaatg
     6361 agacaaaaag cacttgaagc ttttaaaaga ttaggtgtga ctattcccct agacatcccg
     6421 attggtaact tgtctgttgg acaacaacaa atgattgaaa tcgccaaaag tctcctaaac
     6481 caactctcta ttttggtaat ggatgaaccg actgctgctt taacagatcg tgaaactgaa
     6541 aatcttttcc gagttatccg aggtttaaaa caagaaggag tgggcgttgt ctatatctcc
     6601 catcgaatgg aggaaatttt taaaatcact gacttcgtaa ccgtcatgag agatggtgtc
     6661 attgtagata caaaagaaac atcactcaca aattctgacg aattggttaa aaaaatggtt
     6721 ggccgaaaat tggaagatta ctatcctgaa aaacattctg aaattgggcc tgttgctttt
     6781 gaagttagta acctctgtgg agataatttc gaggacgttt ccttttatgt ccgtaaaggc
     6841 gagattcttg gattttctgg attaatggga gctgggcgta ctgaagttat gcgaactatc
     6901 tttggaattg ataaaaagaa gtctggtaag gtgaaaattg ataaccaaga aatcactatt
     6961 actagcccaa gccaagctat caagcagggc attggttttt tgaccgaaaa tagaaaagat
     7021 gaaggtctca tcctagactt caatatcaag gacaatatga ctctccctag cactaaagat
     7081 tttagcaaac atggtttctt tgatgaaaaa acaagcacaa catttgtgca acaattgatt
     7141 aatcgcctct acattaaatc agggagacca gatttagagg ttggaaatct ttctggaggt
     7201 aaccaacaaa aggtcgttct cgctaaatgg attggtattg ctcctaaagt cttaattctt
     7261 gatgagccaa cacgtggtgt tgatgtcgga gctaaacgag agatttacca attaatgaat
     7321 gaactagcag acagaggtgt tccaattgtc atggtatcat cagacttacc tgaaattcta
     7381 ggtgttagtg accgcatcat ggtaatgcac gaaggtcgaa tcagtggaga actcagtcga
     7441 aaagaagctg accaagaaaa agttatgcag ctagccacag gaggtaagta aagtgaaaaa
     7501 tatgatgaaa tatatatcag agttaacaac cctgattgca ttagctggtt taatggttat
     7561 tatcacactc attaatccta atttcctcac aaccaataat ctactcaatt tattattaca
     7621 agtaactgca aatggtttta tcgctttcgg gatgaccttc gttattttga ctggcggtat
     7681 tgatttatca gtcgggtcca tattagccct atctagtgca ttaacagctg gattaatcgg
     7741 ccacggagta cccgttggta ttgctatcgt tttatctgtt gccttaggtg gcatcttggg
     7801 aatgctaaac ggactcttca tttcttacgg aaaattagca ccatttatcg ttacactggc
     7861 aacaatgact atctttagag gagcaacgct agtttattca aacggcaacc caattaccgc
     7921 tggattatcc gatagttttc tattccaatt tttgggacaa ggctatattg ttggtatccc
     7981 attcccagtc attctcatgt ttcttacttt tattattttg tatattttgt tacataaaac
     8041 agccttcggg aaatctgttt acgctttagg aggtaatgaa aaagcagcgt atatctcggg
     8101 tattaagtta aacaaagtta aaattatcat ctacactatc tcaggtatca tggcttccat
     8161 ttctgggtta attatcacat cacgccttag ctcagcacaa ccaacagctg gagcaagtta
     8221 tgagatggat gctattgccg ccgttgtctt aggtggaacg tctttatctg gtgggaaagg
     8281 acgtatcatt ggaacattga ttggtgctct cattattggt gtcctgaaca atggattaaa
     8341 catcattggt gtctctgcat tttggcaaca agttgtgaaa ggtatcgtta ttttaatggc
     8401 tgttttactt gatcgcttta aagtagcaaa atagtcttta ctctaatttc tataagttca
     8461 ttatatttta tctaaaggag tttcaaatga aatttggaaa aaaacttggc tttctagcct
     8521 tattgatgtc tattgtcctc attctcggtg catgtggtaa aactggactt ggaaattctt
     8581 ctgggaattc tacaaaaaat gtaaccaaaa aatctgctaa agatttgaaa ctaggtgtat
     8641 ctatttcaac aactaacaac ccctactttg ttgcgatgaa agacggtatt gacaaatatg
     8701 cgagcaataa aaaaataagc attaaggtag ctgatgctca agatgacgcc gcacgtcaag
     8761 cagatgatgt tcaaaacttt attagtcaaa acgtagatgc tattttgatt aatcccgttg
     8821 actcaaaagc tattgtaaca gctattaagt ctgcaaacaa cgctaatatt cctgttattc
     8881 taatggaccg tggtagtgag ggaggcaaag tattaactac tgtcgcttca gataacgttg
     8941 ctgctggtaa gatggctgcg gattatgccg ttaaaaaact aggcaaaaaa gcaaaagcct
     9001 ttgaattatc tggagtacca ggtgcctctg ctactgtaga ccgtggtaaa ggatttcatt
     9061 cagttgcaaa atctaaactt gacattcttt caagccaatc tgccaatttt gaccgcgcca
     9121 aagctttgaa tactacacaa aatatgattc aaggacataa agatgtccaa attattttcg
     9181 cacaaaatga tgaaatggca cttggggctg cacaggctgt caaatctgct ggcttgcaaa
     9241 atgttcttat cgtaggaatt gatggtcagc cagatgccca tgatgccatt aaaaaaggtg
     9301 atatttcagc cactattgca caacaaccag ctaagatggg agaaatagct attcaagcag
     9361 caattgatca ttataaaggc aaaaaagtag agaaagaaac gatttctcca atttaccttg
     9421 ttaccaaaga caatgttgaa aaatacaatt ggtaaaagta cacaaaaagt cgtgaaaatt
     9481 cacgactttt tatattataa ttttgctaac atattatcga tatgttgaac tgatttttct
     9541 ttacctaata aataaatagt atctggtaac tctggtccat gcatttcacc agaaaccgca
     9601 atacgaatag gcataaatag gttcttacct ttgatgcctg tttctttttg aacagcctta
     9661 atttgaggga aaatagtatc tctagtgaat tcttcgtcag aaagcgatac taacttttcc
     9721 ttaaaagcag ataagactgt tgggactgtt tctgctgcca ttacttcttt ttcggcttct
     9781 gttaattctg gaaaatctgc aaagaaaagg tctgttaatg gtacaatctc atcagccgat
     9841 tttaattgag gtttataaag ctcaactaat ttttcagctt tgtctgtaag tcgaccagct
     9901 tcttctaaga atggtttgca aagtgcaaaa acactctcaa aatcagcatt ttttaggtaa
     9961 tcattactca tccaatccat cttcttctga tcgaatgctg ctggtgattt gctaagacga
    10021 ttttcatcaa atagattaat taattgctcg cgtgagaaga tttcttcttc tccaccagga
    10081 ttccaaccaa gaagggcaat gaaattaaag acagcttccg acatataacc ttttttacga
    10141 tagtcttcga taaattgaag agtatttgtg tcgcgttttg ataatttctt accagtctct
    10201 gagtttataa tcaatgtcat gtgaccaaat tgtggcgctt cccaaccgag tgcttcataa
    10261 accattaatt gttttggcgt atttgcaata tggtcatcgc cacgaataac atgcgaaatt
    10321 tgcatgtcat gatcatcaat gactactgcg aaattgtaag ttggataacc atctttcttc
    10381 tgaataaccc agtctccgcc gatattagag ccttcaaact caatgtcacc ttttaccata
    10441 tcagtccact tataaatccc agattcatta acagcaaggc gtactgtcgg aatgatgcct
    10501 gctgcttcac gttctgcaat gtaagcttct ttttcagttt ctgacattcc gataaattcg
    10561 ttgatatagc gaggtgtttc gcctgccaat tcttggcgtt cacgctctgc tgctaattct
    10621 tcctcagtca cataggactt gtaggcttta ccttctgcta acaactgatc aatataacgt
    10681 tggtagagct caaggcgttc tgattgacga taattttcat gtgtttcagg gctttcatcc
    10741 cagtccatac ctagccaacg taagttctcc aattgagagc gttcaccatc ttcgacgtga
    10801 cgttttcggt cagtatcttc aatacgaata acaaaatccc caccatgatg gcgagcataa
    10861 aggtagttaa agagcgcagt acgcgcattt ccgatgtgta acaatcctgt tggacttggt
    10921 gcataacgca cacgaatctt attagccaaa attctactcc tatattttct gatagtaaac
    10981 tttttcagtt taactgaatt agtcaagggg gcaaaacatc attcaaactc ttacagatga
    11041 ttgccaatca accctattat accactaaat cactaaaaat gataatcagt aataatagtc
    11101 acactaattt tcgataaa
//
LOCUS       DATROQ010000061        10916 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.062, whole genome shotgun sequence.
ACCESSION   DATROQ010000061 DATROQ010000000
VERSION     DATROQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10916
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.062"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            12..1094
                     /locus_tag="VBC94_001439"
     CDS             12..1094
                     /locus_tag="VBC94_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6979701.1"
                     /translation="MLTKFPDQWQDKLTQRQFDDLTDIQNKLFQPITDGDNILGISPT
                     GTGKTLAYLFPTLLKLQPKKSQQLLILAPNSELAGQIFDVTKEWAEPLGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTAGRVFELVKLKKIKMMNINTIVLDEFDELLGDSQYHFV
                     DNIINRVPRDQQMIYISATNKLDNSKLADNTITIDLSDQKLDTIKHYYITVDKRERTD
                     LLRKFSNIPDFRGLVFFNSLSDLGACEERLQFNRASAVSLASDINIKFRKVILEKFKN
                     HDISLLLGTDLVARGIDIDNLEYVINFDIARDKETYTHRSGRTGRMGKEGCVITFVTH
                     KEELKQLKKYATVAELVLHNQKLHLK"
     gene            complement(1127..2380)
                     /locus_tag="VBC94_001440"
     CDS             complement(1127..2380)
                     /locus_tag="VBC94_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO6979702.1"
                     /translation="MKKLIIGIFSLIIIAILAWQGFSFLKHKEIKLQQAVVEKEIRIA
                     EKTVEVVKRQKTERVLFLEPKGYDKSLSADILKWNQKSFEHKKFYDNQYIILRPQLAD
                     SNFANVKKLSIYQILYQKEKGSMFQKSSRLLRTYLLDQNKKPFELDELLAHNISGFKA
                     ILENIAPGTQLKEHDSNKEFLKTGRVTDGLDVKDGKLIINDLKLPLDKLYNVIDESYL
                     KSSDLDLVSNLKAKAPRVALTFDDGPNEKTTPKALEILKRYNAKATFFIMGQSAVGHT
                     DILQRMHAEGHEIGNHTWDHPNLTKLPAEKIKEEIHKTNDLIMKATGQKPVYLRPPYG
                     ATNATVKTVTGLKEMLWSVDTEDWKNHNTQAMMTNIKKQLRPGGVILMHDIHQTSIDA
                     LPTIMDYLTTQGYYFVTVGELYSTP"
     gene            complement(2520..3947)
                     /locus_tag="VBC94_001441"
     CDS             complement(2520..3947)
                     /locus_tag="VBC94_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO6979703.1"
                     /translation="MTKEYQNYVNGEWKSSVNQIEILSPIDDSSLGFVPAMTREEVDH
                     AMKAGREALPAWAALTVYERAQYLHKAADIIERDKEEIATVLAKEISKAYNASVTEVV
                     RTADLIRYAAEEGIRLSTSADEGGKMDASTGHKLAVIRRQPVGIVLAIAPYNYPVNLS
                     GSKIAPALIGGNVVMFKPPTQGSVSGLVLAKAFAEAGLPAGVFNTITGRGSEIGDYIV
                     EHEEVNFINFTGSTPVGQRIGKLAGMRPIMLELGGKDAGIVLADADLDNAAKQIVAGA
                     YDYSGQRCTAIKRVLVVEEVADELAEKISENVAKLSVGDPFDNATVTPVIDDNSADFI
                     ESLVVDARQKGAKELNEFKRDGRLLTPGLFDHVTLDMKLAWEEPFGPILPIIRVKDAE
                     EAVAIANKSDFGLQSSVFTRDFQKAFDIANKLEVGTVHINNKTGRGPDNFPFLGLKGS
                     GAGVQGIRYSIEAMTNVKSIVFDMK"
     gene            complement(4097..5830)
                     /gene="ptsP"
                     /locus_tag="VBC94_001442"
     CDS             complement(4097..5830)
                     /gene="ptsP"
                     /locus_tag="VBC94_001442"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEO6979704.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVEDTNAEEARL
                     DVALQASQDELSVIREKAVESLGEEAAAVFDAHLMVLSDPEMINQIKETIRAKQVNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITERVQDGQ
                     LIAVNGITGEVIIEPTEAQISAFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNENGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAIFEEEKANLLADGVAVAEGIEVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALSECSTMEEV
                     IELQKEYVDFD"
     gene            complement(5835..6098)
                     /locus_tag="VBC94_001443"
     CDS             complement(5835..6098)
                     /locus_tag="VBC94_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEO6979705.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAIEETMTKEGLA"
     gene            6481..6705
                     /locus_tag="VBC94_001444"
     CDS             6481..6705
                     /locus_tag="VBC94_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEO6979706.1"
                     /translation="MAAITVFSKNNCMQCKMTKKFLDQHGADFEEINIDEKPEKIEYV
                     KNLGFSAAPVIEAGNVVFSGFQPSKLKELV"
     gene            6783..8942
                     /gene="nrdE"
                     /locus_tag="VBC94_001445"
     CDS             6783..8942
                     /gene="nrdE"
                     /locus_tag="VBC94_001445"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO6979707.1"
                     /translation="MSLKNIGDVSYFRLNNEINRPVNGQIPLHKDKEALTAFFKENVQ
                     PNSKAFDSITDKIAYLLKYDYLEEAFLNKYRPEFIEELSTKLFDKKFRFKSFMAAYKF
                     YQQYALKTNDGEYYLESIEDRVLFNALYFADGDEELATDLALEMISQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNAIGRSINSALQLSRIGGGVGISLSNLREAGAPIKGF
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLDVFHPDIISFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARNNQEMYLFSPYSIEREYGVPFSYIDITEKYDELVANPNI
                     TKTKINARDLETEISKLQQESGYPYIINIDTANRTNPVDGKIIMSNLCSEILQVQKPS
                     LINDAQEYLEMGTDISCNLGSTNVLNMMTSPDFGKSIKTMTRALTFVTDSSNIEAVPT
                     IKNGNAQAHTFGLGAMGLHSYLAKNHIEYGSPESIEFTDIYFMLMNYWTLVESNNIAR
                     ERQTTFVGFEKSKYADGTYFDKYVSGKFVPQSDKVKSLFTNHFIPEAKDWENLRYAVM
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLATDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSMTLFLRSELPKELYEWKTESKQTTRDLS
                     ILRNYAFNKGVKSIYYIRTFTDDSSEVGANQCESCVI"
     gene            9145..10104
                     /gene="nrdF"
                     /locus_tag="VBC94_001446"
     CDS             9145..10104
                     /gene="nrdF"
                     /locus_tag="VBC94_001446"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO6979708.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSAQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKSEIEEIFEWTNNNEFLQEKARIINDIYANGNALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMHDDDYNYGL"
     regulatory      10189..10287
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10344..10907
                     /gene="thiT"
                     /locus_tag="VBC94_001447"
     CDS             10344..10907
                     /gene="thiT"
                     /locus_tag="VBC94_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEO6979709.1"
                     /translation="MSKNNNTTCLIETAIFAALAMALSMIPDFASWFTPSFGAIPLIL
                     FALRRGTKYGLFAGLIWGLLHFVLSKVYYLSLSQVFIEYILAFISMGLAGVFSAKFKD
                     ALSSSSKTKALSLALSGAILATFVRYVWHYIAGVIFWASYAPKGMSATLYSLSVNGTA
                     GLLTLFFVVISIIILVISYPSFFLPKK"
BASE COUNT         3459 a         2061 c         1795 g         3601 t
ORIGIN      
        1 agaggattct tatgttaaca aaatttccag accaatggca ggataaatta acccagcgtc
       61 aatttgatga tctaacagat attcaaaata aactttttca acctattact gatggtgata
      121 atatccttgg aattagccct acaggtactg gtaaaactct agcatacctt ttcccaacgc
      181 ttcttaagtt gcaacctaaa aagagccaac aactcttaat tttagctcca aattcagaat
      241 tagctggaca aatcttcgac gttacaaaag aatgggctga accattagga ctgacagcac
      301 aactattcct ttcagggtct agtcaaaaac gccaaattga gcgactaaaa aaaggaccag
      361 aaatccttat aggaactgct ggacgcgtat ttgaacttgt gaaactaaaa aaaataaaaa
      421 tgatgaacat taatactatc gtccttgatg agttcgatga attactcggc gactcacaat
      481 atcattttgt ggacaatatt attaatcgcg ttcctcgtga tcagcaaatg atttacatta
      541 gtgccaccaa caaactggac aatagtaaac ttgccgataa cactataaca attgacctat
      601 ctgatcaaaa acttgatact ataaaacatt attatattac agttgataaa agagagcgta
      661 ctgacttact tcgtaaattt tcaaatattc ctgattttag aggtcttgtc ttttttaaca
      721 gcttatcaga cttaggtgca tgtgaagaac gtttacaatt taatagggcc tcggcagttt
      781 ctttagcaag cgatattaat attaaattcc gtaaagttat cttagagaaa tttaaaaacc
      841 atgatatttc attactccta ggaactgatt tggtagctcg tggcattgat atcgacaacc
      901 tagaatatgt gattaatttt gatatcgcac gcgacaaaga aacatataca catcgttctg
      961 gacgaactgg tcgtatgggg aaagagggat gtgttattac atttgtaaca cataaagaag
     1021 agctcaaaca acttaaaaaa tacgccaccg ttgcagaact tgtacttcac aatcaaaaat
     1081 tacacttaaa ataaaaaaag aaacaagtaa cttgtttctt tttttattaa ggcgttgaat
     1141 ataattcacc tacagtaaca aaataataac cctgggttgt cagataatcc ataattgtag
     1201 gcaaagcatc tatggatgtc tggtgaatat catgcatcaa aatcactccc ccaggacgta
     1261 attgtttttt gatattggtc atcattgctt gtgtattatg atttttccag tcttctgtat
     1321 caactgacca caacatttct ttcaatcctg tcactgtttt tacagtagca tttgttgctc
     1381 cgtaaggggg acgtaagtac actggttttt gtcctgttgc tttcatgatt agatcattag
     1441 tcttatgaat ctcttccttg attttctccg ctggtagttt ggttaaatta ggatgatccc
     1501 atgtatgatt accaatctca tgaccttcag catgcatcct ttgtaaaata tctgtgtgcc
     1561 ctacagcact ttgtcccatt ataaagaaag tcgctttagc attatatctt ttaaggattt
     1621 ctaaggcttt aggagtcgtt ttttcatttg gtccatcatc aaaggtaagt gcaacacgtg
     1681 gtgctttagc ttttaagtta gaaactaaat ctagatcact tgattttaaa tatgactcat
     1741 cgatcacatt atataattta tcaagtggta attttaaatc attaataatt aacttaccat
     1801 ctttaacatc tagcccatct gttactcttc cagtttttaa aaattcttta ttgctatcat
     1861 gttcctttag ttgagttcct ggagctatat tttctaaaat agccttaaat ccagatatat
     1921 tatgagctaa aagctcatct aattcaaaag gtttcttatt ttgatcaagt aagtaggttc
     1981 tcaaaaggcg acttgatttt tgaaacatac ttcctttttc tttttgatat aagatttgat
     2041 aaatggataa ttttttaaca tttgcaaagt tagaatctgc taattgagga cgtagaataa
     2101 tgtattgatt gtcataaaat tttttatgct caaaactttt ttgattccac tttaaaatat
     2161 cagcgcttag acttttgtca tatcctttag gttcaagaaa aagaacacgt tcagttttct
     2221 gtcttttcac cacttctacc gttttctcgg ctatgcgaat ttctttttca acaacagctt
     2281 gttgaagttt tatttcctta tgtttcaaaa acgaaaaacc ttgccaagca agaatcgcta
     2341 taataattag actaaaaata cctataatta atttcttcat ataatgactc ttttgttatt
     2401 ttttatgaca attatatcac aaaaataaaa ccactttaga agattatata attctttatt
     2461 tttaatgtaa aagaggttta taaaacgact caactgacaa tcgttgagtc gttttacatc
     2521 tatttcatat caaaaacaat cgattttaca tttgtcattg cttcaattga atatctgata
     2581 ccttgaacac ctgcaccaga tcctttgagt cctaagaatg ggaagttatc aggaccacgt
     2641 ccagtcttat tgttaatgtg aactgtacca acttcaagtt tatttgctat atcaaatgct
     2701 ttttggaaat cacgtgtaaa gactgatgat tgtaatccaa aatcagattt gttggcaata
     2761 gcaacagctt cttctgcatc cttgacacga ataattggga gaattggtcc aaaaggctct
     2821 tcccaagcta gtttcatatc taaagtaaca tgatcaaaca atcctggagt taatagacga
     2881 ccatcacgtt taaattcatt caattctttc gcaccttttt gacgtgcatc tactactaag
     2941 ctttcaataa agtcagctga attatcatca ataaccggtg tcaccgttgc attatcaaat
     3001 ggatcaccta ctgataattt tgctacattt tcagatattt tttctgccaa ttcatctgca
     3061 acttcttcaa cgacaagcac acgcttaatt gccgtacagc gttgtccaga gtaatcataa
     3121 gcacctgcaa cgatttgttt agcagcgtta tcaaggtcag catcagctaa gacgatacct
     3181 gcatccttac cgcccaactc aagcataatt ggacgcattc ctgccaactt accaatacgt
     3241 tgtccaactg gcgttgatcc tgtaaagtta ataaaattaa cttcttcatg ctcaacaatg
     3301 taatctccaa tctcagaacc gcgtcctgta atagtattaa agacacctgc tggaagacct
     3361 gcttctgcaa aagcttttgc taaaacaagt cctgagactg aaccttgtgt tggtggttta
     3421 aacatcacaa cgtttccacc aattagagct ggcgcaattt ttgatcctga gaggttaaca
     3481 gggtaattat aaggtgcgat tgctaaaacg atacctactg gttgacgacg aataacagcc
     3541 aacttatgac ctgttgaagc atccattttt ccaccttcgt cagctgaagt tgataaacga
     3601 attccttctt ctgctgcata acgaataaga tcagctgtcc ttacaacctc agttactgaa
     3661 gcattgtaag ctttagaaat ttcttttgct aaaacagtag caatttcttc tttatcacgt
     3721 tcaataatgt ctgcggcttt atgaaggtat tgtgcacgtt catatactgt taaagcagcc
     3781 caagctggta aagcctcacg acccgctttc atagcatgat caacttcttc tcgagtcatc
     3841 gctggcacga atcccaatga agaatcatca attggtgaca aaatctcaat ctgattaaca
     3901 gatgatttcc attcgccatt gacataattt tgatattctt ttgtcaaagt aaatctcctt
     3961 ttacattgta ataggtttat tctatcactt tatctattta tttcaattat ctgacatatt
     4021 tttcttagaa acttaacaat atccaaactt tcaataaaaa agtagagatt ttttaatccc
     4081 tactttttta ttattctcag tcaaagtcaa cgtattcttt ttgaagttcg ataacttctt
     4141 ccatagttga acactctgaa agagcacgat tagcgtattc ttccatttta gctgtgtcca
     4201 attttttcat taagctacgt gtacgaagta ctgaggttgc tgacattgag aattcatcca
     4261 atcccatacc aacaagaagt ggaaccgcag tttgatcacc ggccatctca ccacacatac
     4321 ctgcccattt accttcagca tgagcagctt tgataacgtt gttaatcaaa cgaaggattg
     4381 atggattgta tggttgatag aggtatgaaa cttgctcatt catacggtcc gcagccattg
     4441 tgtattggat aagatcatta gtaccaattg agaagaaatc aacttctttt gcaaattggt
     4501 ctgcaagcat agctgccgct gggatttcaa tcataatacc aacttcaatg ccttcagcta
     4561 ctgctacacc gtcagctaac aagttcgctt tctcttcttc aaatatagct ttagcagcac
     4621 ggaattcttt aagcaaagca accattggga acatgatgcg tagctgtcca tgaactgaag
     4681 cacgaagaag tgctcggatt tgtgtgcgga acattgcatc accagtttca gaaattgaaa
     4741 tacgcaatgc acggaatcct aagaatgggt tcatctcttt tggaagatca aagtaaggaa
     4801 gctctttatc tccaccgata tccattgtac gaacaacaac tggtttacca ttcataccct
     4861 caagaactgc tttatatgct tcatattgct catcttctgt tgggaaatct tgtgaatcca
     4921 tatataagaa ctctgtacgg taaagaccaa ctgcttctgc tccattttcg ttaacaccct
     4981 caacatcttt aggtgtaccg atattagcag ctaattcaaa atgtttacca tcagcagtaa
     5041 ctgtttgtgc atccttaaga agtgcccatt cagctttttg cttggcataa gcttcacctg
     5101 ctgccttgaa ggctgaaatt tgagcttcag ttggttcaat gataacttca ccagtaatac
     5161 cattaacagc aatcaactga ccatcttgaa cacgttcagt aatatcattt gtaccaagaa
     5221 cagctgcgat ttcgagtgta cgtgccataa tagctgagtg acttgtacgc cctccaatat
     5281 ttgttacaaa agctttaaca aattgtttat ttaattgagc tgtatcagaa ggtgtcaaat
     5341 cgtgtgcaat aacgattgat tcttcattga tagttgccgg gtttggtaat ttaacaccca
     5401 aaagatgtgc taagacacgt ttggcaacat cacggatatc tgctgcacgt tcttgcatgt
     5461 atgggttatc ttccatacct tcaaagatag tgatgaacat atcagtcact tctttaagac
     5521 ctgtttctgc atttacttgt tttgcacgaa tagtctcttt aatttgatta atcatttctg
     5581 ggtcagaaag aaccattaaa tgagcatcaa atacagctgc ggcttcttcg cctaagcttt
     5641 ctactgcctt ctcacggata acagaaagct cgtcttgtga agcttggagt gcaacatcaa
     5701 ggcgagcttc ttctgcgttt gtatcttcga ctgttacagt ctcaaatgac aaatccggtt
     5761 ggacaagtag atatgcttta gcaactgcaa caccatcaga ggctgcgatt cctttaagca
     5821 tttctgtcat tttcttatgc caatccttct tttgtcattg tttcttcgat agctgcaatt
     5881 gcatcatctg catctgcacc ttctgctgaa atagtaacat cagcaccttg accaacacca
     5941 agactcataa cacccatgat tgatttaagg tttactgctt tacctttgta atcaagcgtg
     6001 atgtctgaag caaatttgct agctgtttgg acaagcaaag tagctggacg tgcgtgaata
     6061 cctgtttctg caacaatgtg gaaatctttt gaagccatat tatggttctc cttttgtcat
     6121 ttgtaaatat ttgagtcacc tattggtaac ccttacaagt taaaagtata acatattgct
     6181 ttttattttt caagattaaa tgtctcaaac agaaaaaaac ttgaatgttt tccacagctt
     6241 ttcatattaa aaatgagagt cttagaattt aaaaatagaa ttctaaaaat gattttttta
     6301 tgttcttctg aaaggaattg gaaaacaggc tgaaagcttt taaactaacc ctaaaaacga
     6361 aatatagtgt ctcattttat tttaagacac tatatattgt gttttgttag aaataaaaca
     6421 cttgcttttt ccattctttt tttatatgct attaccatga aaaaaaatta ggaggacaac
     6481 atggcagcta ttactgtttt ttcgaaaaac aactgtatgc aatgtaaaat gactaagaaa
     6541 tttcttgacc agcacggcgc tgattttgaa gaaattaata ttgatgaaaa accagaaaaa
     6601 attgaatatg tgaaaaatct cggcttttct gcggctccag tgattgaagc tggtaatgtt
     6661 gttttttcag gtttccaacc aagcaaacta aaagaacttg tttaagcaaa ttagagtctg
     6721 ggaataaagc aattaattgc tttattccca ttttgtattg ttaagttaag aggataaatt
     6781 caatgagttt aaaaaatatt ggcgatgtat cttactttcg cctaaataat gaaattaacc
     6841 gtcctgttaa tggacaaatc ccactccata aagataaaga agccttaact gcttttttca
     6901 aagaaaacgt acagcctaat tcaaaagctt ttgactcaat cactgataaa attgcttacc
     6961 tccttaagta tgattatctt gaagaagctt tcctcaataa atatcgtcct gaatttattg
     7021 aagaattatc taccaaactc ttcgataaaa agtttcgttt taaatccttc atggcagcct
     7081 ataagtttta tcagcagtat gcattaaaaa caaatgatgg agaatactat ttagaaagta
     7141 ttgaagatcg cgttctcttt aacgctcttt attttgcaga cggtgatgaa gaactagcaa
     7201 ctgacttagc tctagaaatg atttcacaac gctatcaacc tgcaacacca agttttttaa
     7261 atgctggtcg aagtcgtcgc ggagaattag tctcatgttt cttaatccaa gtaactgatg
     7321 atatgaatgc tattggtcgt tcgattaatt cagctcttca attatctcgt attggtggtg
     7381 gggttggaat atcacttagt aaccttcgtg aggctggagc tcctatcaaa ggttttgctg
     7441 gtgctgcttc tggagtcgtt ccagtcatga aattatttga agacagtttt tcttactcca
     7501 accaacttgg acaacgccaa ggtgctggag ttgtttacct tgacgttttc catccagata
     7561 ttatttcatt cctatcaact aaaaaagaaa atgctgatga aaaagtacgt gttaaaacat
     7621 tgtcattagg gattactgta cccgataaat tctatgaact agcacgtaac aatcaagaaa
     7681 tgtacttgtt tagtccttat agtatcgaac gtgaatatgg tgttcctttc agctatattg
     7741 atattaccga aaaatatgat gaattagtcg ctaaccctaa tatcactaaa acaaaaatca
     7801 atgctcgtga tttagaaaca gagatttcta aacttcaaca agaatctggt tatccttaca
     7861 tcatcaatat tgacactgct aatcgtacta atcctgttga cggaaaaatt attatgtcaa
     7921 atctttgctc tgagattttg caagtacaaa aaccaagtct tatcaatgat gctcaagaat
     7981 accttgaaat gggaactgat atttcttgta accttggctc aactaatgtt ttaaatatga
     8041 tgacatctcc tgattttgga aaatcaatta aaacaatgac tcgtgctcta actttcgtta
     8101 ctgactcatc aaatattgaa gctgttccaa ctattaagaa cggaaatgct caagcacata
     8161 catttggact tggagcaatg ggactccact cctatttggc aaaaaatcat atcgaatatg
     8221 gtagtccaga atcaattgaa tttactgata tttatttcat gctaatgaac tactggacct
     8281 tagtagaatc aaacaatatt gcccgcgaac gtcaaacaac atttgtaggt tttgaaaaat
     8341 caaaatacgc tgacggtaca tactttgata aatatgtatc tggaaaattt gttccacaat
     8401 ctgacaaagt aaaatcccta tttactaatc attttatccc cgaagctaaa gattgggaaa
     8461 accttagata cgcagttatg aaagacggac tatatcatca aaatcggctt gcagttgctc
     8521 cgaatggttc tatttcgtat atcaacgatt gttcagcatc tatccaccct attacacaac
     8581 gtattgaaga gcgtcaagag aaaaaaatcg ggaaaattta ttatccagct aatggcttag
     8641 ctacagatac tatcccttat tacacttctg cctatgatat ggatatgcgt aaagtaatag
     8701 acgtctatgc cgcagcaact gagcacgtag accaaggtct ctcaatgaca ctctttttac
     8761 gaagcgaatt accaaaggag ttgtacgagt ggaaaacaga aagtaagcag actactcgcg
     8821 acctttcaat cctacgtaac tatgctttca acaaaggtgt gaaatcaatc tactatatcc
     8881 gtacttttac agatgatagt agcgaagtcg gagctaatca atgcgaaagt tgtgtcatct
     8941 aacgaagaaa ctctacttaa aacatcagaa actagactga gataaactat ctcagtttca
     9001 tgatgttatc ttattttcta aggtattttc atctttattt ttctaaaaat cgataatact
     9061 tgaaatattg ctagatcaga attctatatt ctactcttct tccatcatca ttatataaaa
     9121 acctatttac cattggagaa aaccatgaca acttactacg aagctataaa ctggaacgaa
     9181 attgaagatg ttattgataa atcaacttgg gaaaaactaa ccgaacaatt ttggctcgat
     9241 acacgtatcc ctttatcaaa tgacttagac gattggcgca aactttccgc tcaagaaaaa
     9301 gatcttgttg gcaaggtttt tggaggctta accctacttg ataccatgca atcagaaact
     9361 ggtgttgaag ctattcgtgc cgatgttcgc acgcctcacg aagaagctgt cttaaacaat
     9421 attcaattca tggaatctgt tcacgctaaa tcttattctt caattttctc aactttaaat
     9481 actaaatcag aaattgaaga aattttcgag tggactaata ataatgagtt tcttcaagaa
     9541 aaagcacgta ttatcaatga catttatgct aatggaaatg cccttcaaaa aaaggtggct
     9601 tccacctacc tcgaaacttt ccttttttat tctggctttt tcacacctct ttactatttg
     9661 ggaaataata agttagcaaa tgttgctgaa atcattaaat taattattcg tgatgaatct
     9721 gtacatggta cttatatcgg ttacaaattc cagcttggtt ttaacgaatt accagaagat
     9781 gagcaagaga attttcgtga ttggatgtat gacctccttt atcagctgta tgaaaacgaa
     9841 gaaaaataca ccaagacact ttatgatggc gtaggatgga ctgaagaagt tatgaccttt
     9901 ttacgctaca atgctaataa agctcttatg aatttaggac aagatccttt attcccagat
     9961 acagcaaatg atgtcaaccc aattgttatg aatggtattt caacaggaac atcaaaccat
    10021 gacttcttct ctcaagtagg taatggttac ctacttggta gcgttgaagc tatgcatgat
    10081 gatgactata actatggatt ataagaaaaa gacactgatt tagtgtcttt ttctagttaa
    10141 atacaaattt cttgacaagc aaaccctttc aagatagaat gttagagtaa atacacaagg
    10201 gagtgccttg agctgagatt gcagatatgc aaaatcctct aacctgatct cgttaggacg
    10261 agcgtaggaa ttgtggtttg aattggttaa acatgacaaa tgacaaaaga atctcctttg
    10321 ctgtataaaa aaggagattt tttatgtcaa aaaataacaa cactacctgt cttattgaaa
    10381 ctgctatttt tgcagcgctt gctatggctt tatcaatgat tcctgatttt gcgtcatggt
    10441 tcactccctc ttttggagca attcctctga tactttttgc attacgtcgt ggtacaaaat
    10501 acggtctatt tgctggctta atttggggct tattgcactt tgttctttcc aaagtatatt
    10561 accttagttt atcccaagtt tttatagaat acatcttagc atttataagt atgggacttg
    10621 caggcgtatt ttctgctaaa tttaaagatg cacttagcag cagctctaaa actaaagctt
    10681 tatcattagc attatctgga gcaatattag caacatttgt tcgttatgtt tggcattata
    10741 tcgctggtgt tattttttgg gctagttatg caccaaaagg tatgtctgct acattatact
    10801 cattaagcgt caatggtact gctggtttgt taactctatt ttttgtagtc attagtatta
    10861 ttattttagt tatcagctac cctagcttct ttttacctaa gaaatagaaa aaataa
//
LOCUS       DATROQ010000062        10744 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.063, whole genome shotgun sequence.
ACCESSION   DATROQ010000062 DATROQ010000000
VERSION     DATROQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10744
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.063"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            321..1490
                     /locus_tag="VBC94_001448"
     CDS             321..1490
                     /locus_tag="VBC94_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO6979710.1"
                     /translation="MAWLGNFFTGASFSLVMPFMVLYVEQLGAPSNKVEWYAGLSVSL
                     SALSSALVAPLWGRLADKYGRKPMMVRAGLMMTFTMGGLAFIHSVTGLLILRILNGIF
                     AGYVPNSTALIASQAPQEESGYALGTLATGVTGGMLIGPLLGGLLAEWFGIREVFLLV
                     GTILLISTLMTIFMVKEDFKPISNEETMPTTEVFKSVKSLQILIGLFVTSMIIQISAQ
                     SIAPILTLYIRHLGQTENLMFVSGLIVSGMGFSSILSSPKLGRIGDRIGNHRLLLLAL
                     LYSFLMYVLCSLAQTSLQLGVIRFLYGFGTGALMPSINSILTKIAPRQGLSRIFSYNQ
                     MFSNLGQVLGPFVGSAVSIHLGFRWVFFVTSFIVLANFVWCFINFRKYIRVKEIV"
     gene            1487..1633
                     /gene="rpmG"
                     /locus_tag="VBC94_001449"
     CDS             1487..1633
                     /gene="rpmG"
                     /locus_tag="VBC94_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6979711.1"
                     /translation="MRVKINLKCSSCGSKNYLTSKNKSNHPDKIEVPKYCPKERKVTL
                     HIES"
     gene            1680..1916
                     /gene="secG"
                     /locus_tag="VBC94_001450"
     CDS             1680..1916
                     /gene="secG"
                     /locus_tag="VBC94_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEO6979712.1"
                     /translation="MYNLLLTILLVLSVLLIISIFMQPQKNPSSNVFDSSGSEALFER
                     SKARGFEAFMQRFTGVLVFFWLLIGLVLSILSSH"
     gene            2029..4434
                     /gene="rnr"
                     /locus_tag="VBC94_001451"
     CDS             2029..4434
                     /gene="rnr"
                     /locus_tag="VBC94_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEO6979713.1"
                     /translation="MKEKIIKYLEEHGKTSVNDLAAALDMAGAKAFPKLIKTISNLES
                     HRQLRFDDNGSLSLQKKEAKKKEITVRGLFRANKAGFGFLSIDQDEDDMFIGKNDIAY
                     AIDGDTVEAVVKKPADRLNGTAAEARVVNIVERSLKTLVGKFVLDDERPKYAGYIKSK
                     NQKINQKIYIRKEPVVLDGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVLE
                     VLESMDIVSEFPEDVIAEANAIPDAPTEKDLIGRVDLRQEVTFTIDGADAKDLDDAVH
                     IKLLDNGHFELGVHIADVSYYVTEGSALNREALSRGTSVYVTDRVVPMLPERLSNGIC
                     SLNPNLDRLTQSCIMEIDQNGRVVNHQITQSVINTTYRMTYIAVNDIIAGDEEICSEY
                     ESIVSSVQHMVTLHHTLEAMRTRRGALNFDTSEAKIMVNDKGMPVDIVIRNRGIAERM
                     IESFMLAANETVAEHYARLKLPFIYRIHEEPKAEKLQKFIDYASVFGVQIQGTATKIT
                     QSALQDFMKKVQGQPGSEVLSMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRRY
                     PDLLVHRMIRDYDDKAMDKADHFANLIPEIATQTSSLERRAIDAERIVEAMKKAEYME
                     EYVGEEFEGVVASVVKFGMFVELPNTIEGLIHVTTLPEYYHFNERTLTLQGEKSGKVF
                     RVGQQIKVKLIRSDKETGDIDFDYLPSDFDIVEKVSKSSREGRPNRSSKREHQHRISD
                     RDNKNKNTSKKKASRKPKRNSDSKSHHHKDDRTTGSTKKKTKKPFYKGVAKKGQKRKS
                     KKG"
     gene            4437..4904
                     /gene="smpB"
                     /locus_tag="VBC94_001452"
     CDS             4437..4904
                     /gene="smpB"
                     /locus_tag="VBC94_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEO6979714.1"
                     /translation="MVKGQGNVVAQNKKAHHDYTIVETIEAGIVLTGTEIKSVRAARI
                     TLKDGYAQIKNGEAWLINVHITPYDQGNIWNQDPDRTRKLLLKKREIEKISNELKGTG
                     MTLVPLKVYLKDGFAKVLLGLAKGKHDYDKRESIKRREQNRDIARQLKNYNSR"
     gene            complement(4988..6376)
                     /locus_tag="VBC94_001453"
     CDS             complement(4988..6376)
                     /locus_tag="VBC94_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO6979715.1"
                     /translation="MSKNNNDHTQKSENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SISLTGPSIVLVYAITGAFMFFMMRAIGEMLYQDPNQHTFINFITKYLGPGWGFFSTL
                     SYWISLIFIGMAEITAVGEYVQFWFPEWPSWIIQIVFLAILSSINLIAVKAFGETEFW
                     FAMIKVIAILGLIATGIFMVLTNFDTGHGYHASISNITNHFEWFPKGKLNFFMAFQMV
                     FFAYLAIEFVGVTTSETANPRKVLPKAIQEIPMRIILFYAGSLLAIMAIFPWQQLPVN
                     ESPFVTVFKLAGIKWAAALINFVVLTSAASALNSTLYSTGRHLFQLANESPNALTKAL
                     KLDQLSRQSVPSRAIIASAVIVGASALISVLPGISDAFSLITASSSGVYISIYVLIMI
                     AHWKYRKSPDFMEDGYKMPAYKILSPITLLFFLFVFVSLFLQDSTYIGAIGATIWIIG
                     FGLYSHFKHKSH"
     gene            complement(6505..7431)
                     /locus_tag="VBC94_001454"
     CDS             complement(6505..7431)
                     /locus_tag="VBC94_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6979716.1"
                     /translation="MALLSVIVPCYNEQETVSTFLTEIKKVESEMARYTHFEYIFVND
                     GSTDRTLELLKKAAKQFDNVHYLSFSRHFGKDAALLAGLEHTTGDFITVMDVDLQDPP
                     TLLPEMYLKLQEGYDIVATRRKDRKGEPLIRSLFAKLFYKLINQVSDTKMVDGARDFR
                     LMTKQVVDSILELNEVNRFSKGIFSWIGYDVAYISYENRERIAGKTSWSFFNLLKYSL
                     DGFINFSEIPLAIATWIGTLSSVLSLLAIIFIIIRKLLFGDPVSGWASTVTIVLFMGG
                     IQLLSLGIIGKYISKIFLETKKRPVYIVKEEG"
     gene            complement(7560..7952)
                     /gene="gloA"
                     /locus_tag="VBC94_001455"
     CDS             complement(7560..7952)
                     /gene="gloA"
                     /locus_tag="VBC94_001455"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEO6979717.1"
                     /translation="MPFLHTCIRVKDLDASIAFYQEALGFKEVRRNDFPENQFTLVYM
                     SLEDDPSYELELTYNYDHEAYDLGNGYGHIAVGVDDLETTYDAHQKAGYSVTKISGLP
                     GKPNMFYFIQDPDGYKIEVIRLSQFKAD"
     gene            complement(8040..8714)
                     /locus_tag="VBC94_001456"
     CDS             complement(8040..8714)
                     /locus_tag="VBC94_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6979718.1"
                     /translation="MTKEDIKKQVRRAFDFRMAIRVYNNNDIPKEDMEYILDTAWLSP
                     SSVGLEGWRFLVLDRQTIAKFRDKLKEVAWGAQYQLDTASHFVLLLAEKGAYYNADSM
                     INSLIRRGLGDPAALESRIPLYKSFQENDMKIDSERSLWDWTAKQTYIALGNMMTAAA
                     MIGVDSCPIEGFDYEKVNNILSKEGLIDDKKEAISCMVSFGYRLREPKHSRARKERQE
                     VITWVE"
     gene            8981..9823
                     /locus_tag="VBC94_001457"
     CDS             8981..9823
                     /locus_tag="VBC94_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6979719.1"
                     /translation="METYTLSNTLNIPKIGFGTWQLTEGEEAYKAVTHALKVGYTHID
                     TAQIYGNEHSVGRAIRDSGLARESIFLTTKIWNDKHDYHLAKASIDESLQKLGVDYID
                     LLLIHWPNPKALRENDAWKAGNAGTWKAMEEAYKEGKVKAIGVSNFMKHHLEALFETA
                     EIKPMVNQIILAPGCAQEDLVRFCKGNDILLEAYSPFGTGTIFENESIKAIAEKYGKS
                     VAQVALRWSLDNGFLPLPKSATPKNIEANLDIFDFQLNEDDIATLIQLDSGIKPKDPD
                     NVSF"
     gene            10014..10736
                     /locus_tag="VBC94_001458"
     CDS             10014..10736
                     /locus_tag="VBC94_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6979720.1"
                     /translation="MRLDKFLVECGLGSRTQVKLILKKKQISVNSNSETSPKVQVDEY
                     RDEIKYNGTLVSYEKFVYYMLHKPKGVISATDDPSHKTVLDLLDKTARDKAVFPVGRL
                     DIDTTGLLLITNNGELAHKMLSPKKHVDKCYEAKISGIMTEDDILAFDKGIILKDFTC
                     LPALLEIVEVNQVKKQSLVKITIKEGKFHQVKRMVAACGKEVLELKRLSMGNLQLDKQ
                     LESGQWRRLTIKEIEKLEKHMQ"
BASE COUNT         3670 a         1709 c         2078 g         3287 t
ORIGIN      
        1 cctttactaa atctataacg atgccaatat tattagtaag cctaggaatt atagttgtta
       61 ttttaattat tggctattta gcagttgtag tatttgctcc aaaaactttc cgtttaaaaa
      121 gttaatttta attgtggtat aataacagag gcatgtgata acataggttt agttacataa
      181 aggaaattat aatggaaata acatctcaaa tagatgttaa atctcaataa aactgctaag
      241 atagttagtg gtagaaaagt ttagaaagaa gatgatgttt tatctcagtt gaaacggtta
      301 actggaagca aaatttaaaa gtggcttggc tcggtaattt ttttactggg gcaagctttt
      361 cacttgttat gccttttatg gtattatatg tagaacaatt aggagcacct agcaacaaag
      421 tagagtggta tgcaggccta tcagtatcct tatcagcctt gtcttcagct ttagttgccc
      481 cattatgggg gcgcttagct gataaatatg gtagaaaacc gatgatggta agagcagggt
      541 tgatgatgac ttttactatg ggaggccttg ctttcattca tagtgttaca ggacttctga
      601 ttttacgtat attaaacggc atttttgcag gctatgtacc taactctaca gctttgatag
      661 caagtcaagc tcctcaagag gaatcaggat atgccttagg aaccttagcg acaggagtga
      721 ctggaggtat gttaattggt ccacttttag ggggactttt agcagagtgg tttggtatta
      781 gggaagtttt tttattagtg ggaaccatat tgttaatatc tactttaatg accattttta
      841 tggtaaagga agatttcaaa cccatttcta acgaagaaac catgccaact acagaagtgt
      901 tcaaaagcgt taaaagctta caaattttaa tagggctgtt tgtaacaagc atgattattc
      961 aaatttctgc acaatcaatt gcacctattt taacacttta cattcgtcat ttaggacaga
     1021 ctgaaaactt gatgtttgtt tccggattaa ttgtttctgg tatgggattc tctagtattc
     1081 tgagtagtcc aaagctagga agaatcgggg atcgcatagg caatcatcgt ttgctattat
     1141 tagctctact gtatagtttc ctcatgtatg tcttatgttc attggcacag acttcattac
     1201 agctaggggt tattagattc ctgtatggtt ttggaacagg tgccttgatg ccgagtatta
     1261 attcaatctt gactaaaatt gctccaagac aaggtttatc aaggattttc agttataacc
     1321 aaatgtttag caatttagga caagtattag gtccttttgt cggttcagca gtttctattc
     1381 atttaggatt ccgatgggtt ttctttgtga ctagcttcat cgttttagct aattttgttt
     1441 ggtgttttat taattttaga aaatatatta gagttaagga aattgtgtga gagttaaaat
     1501 taatttaaag tgtagctctt gtggtagtaa gaattattta accagtaaga ataaaagtaa
     1561 tcatcccgat aaaatcgagg ttccaaagta ttgcccaaag gagagaaaag taacactaca
     1621 tattgaatct taataagttt tatgttagaa tgtactttga ccatagtgga ggaattaaca
     1681 tgtataacct attactcacc attctacttg ttttatcagt attattgatt atctcaattt
     1741 tcatgcaacc acaaaagaat ccaagtagta atgtttttga tagtagcggt tctgaagccc
     1801 tttttgaacg tagtaaagct cgaggatttg aagcttttat gcaacgtttt acaggtgttt
     1861 tagtcttttt ctggctacta atcggcttag tattatctat tttatcaagt cattaatgat
     1921 agctaaatac agataagtga ggatggggga tagcccaacc tcactttttg ttagtagaat
     1981 ggttgtagag taataataaa gtgactaaag ttactagaaa aggaaataat gaaagaaaaa
     2041 attataaaat atttggaaga acacggtaaa accagcgtta atgatttagc tgctgcccta
     2101 gatatggcag gcgcaaaagc atttccaaaa ttaatcaaaa caatctcaaa tttggagagc
     2161 cacaggcaat taagatttga cgataatgga agtctttctc ttcaaaagaa agaagctaag
     2221 aaaaaggaaa tcacagtaag gggtcttttt agagcaaata aagcaggatt tggttttctt
     2281 agtattgacc aagacgaaga tgatatgttt atcggaaaaa acgatattgc ttatgctatt
     2341 gatggtgaca cggtagaagc tgttgtaaag aaaccagcgg accgtcttaa cgggactgca
     2401 gcagaagcgc gtgttgttaa tattgttgaa cgtagtttaa agacgcttgt tggtaagttt
     2461 gtcttagacg atgagcgccc taaatacgca ggatatatca aatcgaaaaa tcaaaaaatc
     2521 aatcaaaaga tttatatacg caaagaaccg gtcgttttag acggaacaga aattatcaaa
     2581 gtagatattg ataaatatcc gacacgagga catgattact tcgtagcaag tgttcgtgat
     2641 atcgttggac atcaaggaga tgttggtatt gatgttttag aagtcttgga aagtatggat
     2701 atcgtctcag aatttcctga ggacgttatt gcagaagcca atgctatccc agatgctcca
     2761 acggagaaag atttaattgg gcgtgttgat ttacgtcaag aggtcacttt cactattgat
     2821 ggagcggacg ccaaagattt agacgatgct gtgcatatta aattactgga taatggtcat
     2881 tttgaacttg gagttcatat cgcagatgta tcatactacg ttactgaagg ctctgcctta
     2941 aatcgtgaag cacttagtcg tggtacgtcc gtatatgtca cagatcgcgt tgtaccaatg
     3001 ttaccagagc gtctatctaa cggtatatgt tcattaaatc caaatcttga tcgtttaacg
     3061 cagtcttgta ttatggaaat tgaccaaaat gggagagttg ttaatcatca aattacacag
     3121 tcagtgatta ataccactta tcgtatgact tatatcgctg taaatgatat tattgctggc
     3181 gatgaggaaa tctgctcaga atatgagtct attgtgtcct cagtacagca tatggtcact
     3241 ttacaccata ctttggaagc tatgcgtacg cgtcgtggag cgctgaattt tgacacatct
     3301 gaagcaaaaa ttatggttaa tgataaggga atgccagttg atattgttat tcgcaacaga
     3361 ggcattgctg aacgcatgat tgagtcattt atgttggcgg caaatgaaac ggttgctgaa
     3421 cattatgctc gtctaaaact gccatttatt tatcgtatac acgaagagcc taaagctgaa
     3481 aaattacaaa aattcatcga ttatgcttct gttttcggtg ttcagattca aggaacagca
     3541 actaaaatca cccaatctgc tttacaggac tttatgaaaa aagtacaagg gcagccagga
     3601 agtgaagtgt tatctatgat gttattgcgc tcgatgcaac aagctagata ttctgaacac
     3661 aaccatggac attatggact tgctgcggaa tattacactc actttactag ccccattcga
     3721 cgttatccag acttgttagt tcatcgtatg attcgtgatt atgatgataa agcgatggat
     3781 aaagctgatc attttgctaa ccttatccca gaaattgcaa ctcaaacgtc tagtcttgag
     3841 agacgtgcca ttgatgctga acgtattgta gaggcaatga agaaagccga atatatggaa
     3901 gaatatgttg gtgaggaatt tgaaggtgtg gttgcaagcg ttgttaaatt tggtatgttt
     3961 gtagaattac caaatactat tgaaggattg attcacgtaa cgaccttgcc tgaatattat
     4021 cactttaatg aacgtacgct gacgcttcaa ggtgagaaat ctgggaaagt tttccgtgtt
     4081 ggtcaacaaa ttaaggttaa gttgattcgt tctgataagg aaactggtga tatcgatttt
     4141 gattatttac catcagattt tgatattgtt gaaaaggtaa gtaaaagctc gcgtgagggt
     4201 cgtcctaata gaagttctaa acgagaacat cagcatagaa tttctgatcg tgataataag
     4261 aataaaaata catctaagaa aaaagcatct cgaaaaccca aaaggaactc ggatagcaaa
     4321 tcacatcatc ataaagacga tagaacaaca ggttcaacca agaaaaaaac gaaaaaacct
     4381 ttctataaag gcgttgctaa gaaaggtcag aaacggaaat ctaagaaagg ttaagtatgg
     4441 ttaaaggtca aggaaacgtt gtagcacaaa ataaaaaagc gcatcacgac tatactatcg
     4501 ttgaaacaat agaagcagga attgttttga caggaacaga aatcaaaagt gtccgcgcag
     4561 cgcgtattac attgaaagat ggatatgcac aaataaaaaa tggtgaagcg tggttgatta
     4621 atgttcatat tacaccatat gatcaaggta acatatggaa tcaagatcct gatcgtacac
     4681 gaaagttatt actaaaaaaa cgtgagattg aaaaaatatc aaatgaactg aaagggactg
     4741 gtatgactct ggtacctcta aaagtttact taaaagatgg ttttgctaaa gttctattgg
     4801 gtttggcaaa aggaaaacat gattatgata agcgagagtc tatcaaacgt cgtgagcaaa
     4861 accgtgatat tgcccgtcag ttaaaaaact ataattcacg atgattatca aaaggttagg
     4921 gaattgccta accttttttg gacgaaaaaa gtgaagttga ttgaccaact tcactaataa
     4981 tatagactta atgtgattta tgtttgaagt gactatataa accaaatcca ataatccaga
     5041 tggttgctcc aatagccccg atgtatgttg aatcttgcaa gaagagagaa acgaatacaa
     5101 agaggaagaa gagaagtgtg attggtgaaa ggattttgta ggcaggcatt ttataaccat
     5161 cttccataaa atcaggtgat ttacgatatt tccaatgtgc aatcataata agaacataga
     5221 tagaaatgta aacaccagaa gatgatgcag taattaagga gaaggcatct gaaataccag
     5281 gtaaaacact gataagtgct gaagctccaa cgattacagc tgatgcaata atagctcgac
     5341 ttggaacgct ttgacgagat aattggtcta attttaacgc ttttgtaaga gcatttggtg
     5401 attcattagc caattggaat aaatgacgcc ctgttgagta caaagtagaa ttaagagcag
     5461 atgctgcaga cgttaggaca acaaagttaa tcaaagcggc agcccactta atcccagcta
     5521 gtttgaatac tgtaacgaaa ggtgactcat taactggtaa ctgttgccat gggaagatgg
     5581 ccataattgc taataatgaa cctgcgtaga ataaaataat acgcatcgga atttcttgga
     5641 ttgcttttgg taaaacttta cgtggattag ctgtttcaga agttgttact ccgacaaatt
     5701 caatagctaa ataagcaaag aataccattt ggaaagccat gaagaaattc aatttacctt
     5761 tagggaacca ttcaaaatga ttggtgatat ttgagattga agcatggtat ccgtgaccgg
     5821 tgtcaaagtt tgtcaatacc ataaagatac cggttgctat taaaccaaga attgcaataa
     5881 cttttatcat agcaaaccaa aactcggttt ctccgaatgc ttttacagca attaagttga
     5941 tagaacttag gatagctaaa aaaacaattt ggataatcca ggatggccat tcagggaacc
     6001 aaaattgaac atactctcct acggctgtaa tctctgccat accgataaat attagggaaa
     6061 tccaataaga aagtgttgag aaaaatcccc aaccgggtcc taaatatttg gtaatgaaat
     6121 taataaaagt atgctgattt ggatcttgat aaagcatctc tccaatagca cgcatcatga
     6181 agaacatgaa agcgccagtg atagcataga caagaactat tgaaggaccc gtcaggctaa
     6241 ttgaacgacc tgcaccaaga aaaagcccag ttccgattgt ccctgcaatc gcaattaact
     6301 gtacgtgacg attttctagg ccgcgtacca taccattctc agatttttga gtgtggtcat
     6361 tattattttt tgacattata aatactccat ctatttttga aaaactcctt cattatataa
     6421 tttttttgca aaattcgcaa taattactat acaatatttt tctatgataa aatctcctag
     6481 ctaagtgagc taagagattt caattcatcc ctcttccttg acaatataga ctgggcgttt
     6541 tttagtttct aggaaaatct tagagatgta tttgcctata attcctagcg acaataactg
     6601 aataccaccc ataaaaagta caatggtaac tgtgcttgcc caacctgaaa cgggatcccc
     6661 aaatagtaat tttcgaatta taataaaaat gatagctaaa agtgatagta ctgaactaag
     6721 ggttccaatc caggtagcta tagctaatgg aatttctgag aagttgatga aaccgtctag
     6781 agagtatttt aagagattaa agaaactcca tgaggtctta cccgcaattc gttctcgatt
     6841 ctcataacta atataagcga catcatatcc aatccaggaa aaaatccctt ttgagaagcg
     6901 attcacttca tttagctcga gaatactgtc aacaacttgt tttgtcatga gacgaaaatc
     6961 acgcgcacca tcaaccattt tagtatctga cacttgattg attaatttat aaaaaagctt
     7021 ggcaaaaaga gagcgtatca acggctcccc tttacgatct tttcgccttg tagcgacaat
     7081 atcataacct tcttgaagtt ttaggtacat ttctggtaat aaagtgggtg gatcttgcaa
     7141 gtcaacatcc ataacagtga taaaatcacc agtagtatgt tctaaaccag ccaataaagc
     7201 agcatcttta ccaaaatggc gtgaaaatga aaggtaatga acattgtcaa attgtttagc
     7261 tgctttctta agaagttcga gtgtacgatc ggtagaacca tcgtttacga aaatatattc
     7321 aaaatgggtg taacgagcca tttcggattc aacttttttg atttctgtta aaaaagttga
     7381 gacagtctct tgttcattat aacaagggac aataactgat aataaagcca tttgtaattc
     7441 tccgtatcat tcttgcttaa attttaacat aatatgggaa aaaatagaaa gattataagg
     7501 taatcataaa aaggactagg tttttgcctc agtccttaac ttaatatgat gagatgttat
     7561 tagtcagctt taaattgact gagtcgaata acttcaattt tatagccatc tggatcttga
     7621 ataaagtaga acatattagg tttacctggt aatccagaaa tttttgttac agagtaacct
     7681 gctttctgat gggcatcata agtagtttcc aaatcgtcta caccaacagc aatgtgacca
     7741 tacccatttc ctaaatcata agcttcgtga tcatagttat aggttaactc taattcgtaa
     7801 ctagggtcgt cctctaggga catatagact aaagtaaatt gattttcagg aaagtcatta
     7861 cgacgaactt ccttaaaacc aagtgcttct tgataaaaag caattgaggc gtccaaatct
     7921 ttaacgcgaa tacaagtatg taaaaatggc atagtctctc tcctatttct ttattaaacg
     7981 tattatgtca aaataaaatt atcttttcaa aaatattgat tataaattag gctgtctatt
     8041 tactcaaccc aagtaatgac ctcttgacgt tctttcctag cacgggaatg tttaggttca
     8101 cgcaaacgat aaccaaatga aaccatacaa gaaatagctt ctttcttatc gtctattaac
     8161 ccttctttac taagaatatt attgaccttt tcatagtcaa atccttcaat tgggcatgaa
     8221 tctacgccga tcatagcagc agccgtcatc atattaccaa gtgcgatata agtttgtttt
     8281 gctgtccaat cccaaagaga acgttcagaa tcaattttca tatcgttttc ttggaaggat
     8341 ttgtagagag gtatcctact ctcaagggcg gctggatcac ctagaccacg tcgaatgaga
     8401 ctgttaatca tgctatcagc gttataataa gcaccttttt cagctagtag taaaacaaag
     8461 tgactagcag tatctagttg atattgtgct ccccaagcaa cttccttaag tttatctcga
     8521 aattttgcaa ttgtttgtct atctagtact aaaaatcgcc agccttctaa accgactgaa
     8581 gaaggactca accaagcagt gtctaaaata tactccatat cttctttagg aatatcatta
     8641 ttattataaa cacgaatcgc catacgaaaa tcaaaagctc ttcgaacttg ttttttgata
     8701 tcttcttttg tcattttaga tcctcctgtt attaatgcta tcatatcaca atttagaaaa
     8761 aatctcagca ggcggtatcg tggcaaagta gagctacaaa attctaatga ctgtcagaaa
     8821 aataaaggga atgaacaaaa tgatagggtt ttgatgaaga aagttatata attttacata
     8881 aggaggggat aataccatat atcaaactat catttagatg tttaaaatag atgtttttca
     8941 ctatgaaatt catttttgtc acatagaaaa ggagattgtt atggaaacat atactctatc
     9001 gaatactctt aacattccta aaattggttt tgggacatgg caactaactg aaggtgaaga
     9061 agcttataaa gcagtgactc atgctttaaa agttggttat actcatattg atactgctca
     9121 gatttatggt aatgaacata gtgttggacg tgctataaga gacagtggtt tggcgcgaga
     9181 aagtatcttt ttgacaacta agatttggaa tgacaaacat gactatcatt tagccaaagc
     9241 ttctattgat gaatctctgc agaaattagg tgttgattat attgatcttc ttttgattca
     9301 ttggccaaat ccaaaagctc ttcgtgaaaa tgatgcgtgg aaagctggga atgcaggcac
     9361 ttggaaagcg atggaagaag cttataaaga aggaaaggta aaagctattg gtgtttcgaa
     9421 tttcatgaaa catcacttag aggctttatt tgaaaccgcc gaaataaaac ctatggttaa
     9481 tcaaattatc ctagcaccag gttgtgctca agaagatttg gttcgcttct gtaaaggaaa
     9541 tgatatctta ttagaagctt acagtccatt tggaacaggt actatttttg aaaatgaatc
     9601 tataaaagct attgctgaaa aatatggtaa atcagttgca caagtagctc ttcgctggtc
     9661 acttgataat ggctttttac cattacctaa gtcagcgact cccaaaaata ttgaagcaaa
     9721 ccttgatatt tttgattttc aattaaacga agatgatatt gctaccttaa tacaactaga
     9781 ttcaggtatt aaacccaaag atccagataa tgtctcattt taataagaaa agatggaaat
     9841 gtatttccat cttttttatg tgggatagtt ccttaaagtt aagaaaaaca ttaagacctc
     9901 cattgacgag gagaatatat taatgatagt attactagaa aagtagtaat aaaggatgag
     9961 tatggtaaaa tcctttacaa tatatcatta tcgaaagagg ttataacatc taaatgagac
    10021 ttgataaatt tttagtagaa tgcggacttg gttcacgtac tcaagtcaaa ttaatattga
    10081 aaaaaaagca gattagtgtt aatagtaata gcgagacatc tccaaaagta caggtagatg
    10141 aatatagaga tgagattaaa tataatggaa ctctagtgtc atatgaaaag tttgtttatt
    10201 atatgcttca taaacccaaa ggtgttatat cagcaacgga tgatccttcg cataaaaccg
    10261 tattagattt gctagacaaa actgctcgag ataaagctgt ttttccagtg gggcgtttag
    10321 atattgatac aacaggcctt ttgttaataa ctaataatgg ggaattagcg cataaaatgt
    10381 tatcacctaa aaaacacgtt gataaatgct atgaggcaaa aatctcaggg ataatgacag
    10441 aagacgatat tttggcattt gataaaggga ttattctgaa agattttact tgtttaccag
    10501 cattattaga aattgtggaa gtaaatcagg tgaagaagca gagtcttgtt aaaatcacta
    10561 ttaaggaagg aaaattccat caagttaaac gtatggttgc tgcgtgtggc aaggaagtct
    10621 tagagctaaa gcgtctaagt atgggaaatc tacaattaga taaacagcta gaaagtggac
    10681 aatggagacg gctaacaata aaagaaatag aaaaattaga aaaacatatg caataatata
    10741 aaat
//
LOCUS       DATROQ010000063        10658 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.064, whole genome shotgun sequence.
ACCESSION   DATROQ010000063 DATROQ010000000
VERSION     DATROQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10658
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.064"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(140..697)
                     /gene="lepB"
                     /locus_tag="VBC94_001459"
     CDS             complement(140..697)
                     /gene="lepB"
                     /locus_tag="VBC94_001459"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6979721.1"
                     /translation="MVKRDFIRNIILALIAVLILILLRYFVFATFKVHKDATNSYFSN
                     GDVVVVNRNRTPKYKDFIVYKVGKIFYISRVIGEPNQKVRVMDDILYLNDVFKDEPYI
                     EKMKNAYSEKKDGQMPFTSDFSVETLTRNKESRVPKGSYLVLNDNRQNKNDSRKFGLI
                     KEKDIRGVITFKVYPLSEFGFTASE"
     gene            complement(868..2370)
                     /gene="pyk"
                     /locus_tag="VBC94_001460"
     CDS             complement(868..2370)
                     /gene="pyk"
                     /locus_tag="VBC94_001460"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEO6979722.1"
                     /translation="MNKRVKIVATLGPAVEFRGGKKFGESGYWGESLDVEASAEKIAQ
                     LIKEGANVFRFNFSHGDHAEQGARMATVRKAEEIAGQKVGFLLDTKGPEIRTELFEDG
                     ADFHSYTTGTKLRVATKQGIKSTPEVIALNVAGGLDIFDDVEVGKQILVDDGKLGLTV
                     FAKDKDTREFEVVVENDGLIGKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVNEVRAICEETGNGHVKLFAKIENQQGIDNIDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIITKVNAAGKAVITATNMLETMTDKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDKNAQTLLNEYGRLDSSAFPRNNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRSLMINWGVIPVLAD
                     KPASTDDMFEVAERVALEAGLVESGDNIVIVAGVPVGTGGTNTMRVRTVK"
     gene            complement(2419..3441)
                     /gene="pfkA"
                     /locus_tag="VBC94_001461"
     CDS             complement(2419..3441)
                     /gene="pfkA"
                     /locus_tag="VBC94_001461"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEO6979723.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINQGYYGM
                     VTGDIFPLDANSVGDTINRGGTFLRSARYPEFAELEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGLPGTIDNDIVGTDYTIGFDTAVATAVENLDRLRDTSASH
                     NRTFVVEVMGRNAGDIALWSGIAAGADQIIVPEEEFNIDEVVSNVRAGYAAGKHHQII
                     VLAEGVMSGDEFAKTMKAAGDDSDLRVTNLGHLLRGGSPTARDRVLASRMGAYAVQLL
                     KEGRGGLAVGVHNEEMVESPILGLAEEGALFSLTDEGKIVVNNPHEADLRLAALNRDL
                     ANQSSK"
     gene            complement(3522..6626)
                     /locus_tag="VBC94_001462"
     CDS             complement(3522..6626)
                     /locus_tag="VBC94_001462"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEO6979724.1"
                     /translation="MFAQLDTRTVYSFMDSLVDLKTYVSKSKSLGYQTIGILDHSNLY
                     AAYHFIQEAQKANLRPIVGFSFDIVVENRSIEVYCIAINTVGYKNLLKLSTAQMSEKM
                     SLNLLTEHLEGVQLILPYQDALDQLNLPFDYVIGVNLTSPQIPYTKPIIAIDTVRYFQ
                     KNDIETLQMLHAIRDNVLLKDAQYASKNQELKPCQEMTLAFKKRFPEALANLESLVEN
                     VVYHFDSDFKLPIFNREIPAGEELRTLTQNNLKSKGLWSDAYQERLEKELTIIHKMGF
                     DDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVSYALNITGIDPVKHNLIFERFLNEE
                     RYSMPDIDIDLPDIYRGEFLRYVRNRYGSMHSAQIVTFSTFGAKQAIRDVFKRFGASE
                     YELTNITKKIHFRDNLTSVYNRNLAFRQIIDSKIEYQKAYDIAKRIEGNPRQTSIHAA
                     GVVMSDDLLTDHIPLKNGEDMMITQYDASSVEANGLLKMDFLGLRNLTFVQKMKEKVD
                     KDYGISIQLEAIDLEDKETLKLFAAGQTKGIFQFEQSGAINLLRRIRPECFEDVVATT
                     SLNRPGASDYTENFINRRFGKEKIDLVDPVIAPILQPTYGIMLYQEQVMQIAQTYAGF
                     TLGKSDLLRRAMSKKNSKEMQKMSQSFLEGAVSKGHRQEDARLIFERMAKFAGYGFNR
                     SHAFAYSALAFQLAYFKAHYSDVFYDIMINYSNSDYLIDAIDFGFVIEKPSINTISYR
                     DRIYKKKIYLGLKNIKAVPNDLAYWISKNQPFQSIEDFLMRLPQQFQKSGFISPLIAI
                     GAFDEFDNNRRKITSNLDSLFTFVNELGSLFADTSYHWLEVEDFSNSEKYEMEQDILG
                     VGISPHPLVGISQKASRPFIPISQVQENSEATILVQLKQVKIIRTKSSGQQMAFLTVM
                     DINSKMDITVFPETFNIVRDDLQEGKYYYLHGKIQKRDERLQMVLNGVQEATEERFWI
                     LLKNHDNDKKISEILSKYKGHIPVYLHYETTKETIQSKVHLVRKDSGLALDLSEFVVK
                     TVYQ"
     gene            6771..7139
                     /locus_tag="VBC94_001463"
     CDS             6771..7139
                     /locus_tag="VBC94_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6979725.1"
                     /translation="MAWEFNEKSPIYSQIAEHIKMQIVSQEIKSGDQLPTVRELAQEA
                     GVNPNTMQRAFTELEREGMVFSQRTSGRFVTEDNLLIGKIRQQVAKAELATFVNNMKK
                     IGYKLDEITVALDHFIKEIN"
     gene            7140..7838
                     /locus_tag="VBC94_001464"
     CDS             7140..7838
                     /locus_tag="VBC94_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979726.1"
                     /translation="MTQLLQLHHVTKKYHKHIAVNDVTVSIPTGKIIGLLGPNGSGKT
                     TIIKMINGLLQPDKGDIVIDGYRPSVETKKIISYLPDTSYLQENMKIKDVVTLFEDFY
                     NDFNSKVAYQLFEDLNLNPKERLKNLSKGNKEKVQLILVMSRKARLYILDEPIGGVDP
                     AARDYILKTIISNYSNDASVLISTHLISDIEPILDEVIFLKEGEIDLQGNADDLREEH
                     NCSIDALFRERFKK"
     gene            7850..8632
                     /locus_tag="VBC94_001465"
     CDS             7850..8632
                     /locus_tag="VBC94_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter (permease)"
                     /protein_id="HEO6979727.1"
                     /translation="MFGKLLKYELKSVGKWYLTLNAAVLLVSIILGLVLKALGGNFST
                     DTNSTSAQIFTIILVLLLAMVISGSLLSTLAIIIKRFYSNIFGRQGYLTLTLPVTTNQ
                     IICSKLLASLLWSIFNIFIVIIGIILVILPLVGIGQFVVAFPEIYKIISSSNAPLFIA
                     YFFLSYVAGTLLIYLSIAVGQLFTNKRVLMGIVSYFGISLLITFLTLIIDSIFHIDLF
                     NSHANATFSQPVLLYNILVSIVEIAIFYMLTHSIIKYKLNIQ"
     gene            complement(8669..9280)
                     /locus_tag="VBC94_001466"
     CDS             complement(8669..9280)
                     /locus_tag="VBC94_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEO6979728.1"
                     /translation="MNMKLSKRYRFWQKVIKALGVLALIATLVLVVYLYKLGILNDSN
                     ELKDLVHKYEFWGPMIFIVAQIVQIVFPVIPGGVTTVAGFLIFGPTLGFIYNYIGIII
                     GSVILFWLVKFYGRKFVLLFMDQKTFDKYESKLETSGYEKFFIFCMASPISPADIMVM
                     ITGLSNMSIKRFVTIIMITKPISIIGYSYLWIYGGDILKNFLN"
     gene            9373..9722
                     /gene="ssrA"
                     /locus_tag="VBC94_001467"
     tmRNA           9373..9722
                     /gene="ssrA"
                     /locus_tag="VBC94_001467"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            9991..10629
                     /locus_tag="VBC94_001468"
     CDS             9991..10629
                     /locus_tag="VBC94_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEO6979729.1"
                     /translation="MASILKKHLEKRLELKNDPMRTDMSTGASYPKYGFSVGKYTYGY
                     QQFFYEGVNLKEIGAFCSIAQNVTITGMNHPTDHITTNPFIYYKSRGFINEDRADLID
                     EKKNGKVIIGNDVWIGTNVTILPSVTIGNGAIIEAGSVITKDIPDYAVVAGTPAKIIK
                     YRFSEEEITLLNASQWWNWSDEAIKEHISEFSDKREFFNTLKSISENKNHKL"
BASE COUNT         3519 a         2026 c         1659 g         3454 t
ORIGIN      
        1 aaaaaaattg gtatagtctt tttgattttc tgtgataacg agataaacgc tatcaaaaat
       61 tctatctatc ttaaaaaaat atatttctta gccattttat tacataaaaa gaatggtatc
      121 aaataatacc attcttttac tactctgatg ctgtaaaacc aaattcactt agagggtata
      181 ctttaaaggt aataacacca cgaatatctt tctctttgat taatccaaat tttcgactat
      241 catttttatt ttgtcgatta tcatttaaca caagataaga acccttagga actctgcttt
      301 ctttatttcg tgttaatgtt tcaactgaga agtcggaagt aaagggcatt tgaccgtctt
      361 tcttttcaga ataagcattt ttcatttttt caatgtaagg ctcatccttg aaaacatcat
      421 tcaaatataa aatatcatcc attactctca ctttttgatt tggctcacca ataacacgac
      481 taatatagaa aatctttcca accttataga caataaagtc tttgtactta ggcgtcctat
      541 tacgattaac aacaacaaca tctccgttgc taaaataaga atttgtagca tccttatgta
      601 ctttaaaggt tgcaaaaaca aaatatctca agagaatcaa aatcaataca gcaatcaatg
      661 ctaaaataat atttctaata aaatcgcgtt taaccataac tactcctaat cttttttcca
      721 ttatatcatg ttttcataga taaaaaaagg agggtaatta ttaatatcat atcctattct
      781 agtcacaata atcgaataat aaaaagcaaa acatataaaa aacccattat ttaaggtatt
      841 cttaaataat aggtttgaat tcaaaaatta tttaacagta cgaacacgca ttgtgttagt
      901 tccacctgta cctacaggaa cacctgcaac gataacgata ttatcgcctg attcaacaag
      961 tcctgcttca agtgctacac gttctgcaac ctcaaacata tcatctgtag atgctggttt
     1021 gtctgcaagg acagggataa caccccagtt aatcatcaat gaacgttgta ctttttcatc
     1081 aaatgtaaca gccaaaatgt ctgcatctgg acggaattta gaaatggcac gagctgtatt
     1141 acctgtttca gtaattgtta caacaagttt gatatccatt gagtgtgttg catctttaac
     1201 cgcagatgca ataacatcag ttttgttatt acgtgggaat gcagatgagt ctaagcgacc
     1261 atactcattg agtaatgttt gagcattttt atcaatagta gccattgtac gaactgactc
     1321 aactgggtat ttaccattag ctgactcacc tgaaagcatt gtagcatcag taccatcaat
     1381 aacagcattg aagacatcag atacttctga acgagtcgca cgtggtttat cagtcattgt
     1441 ttcaagcata tttgttgctg taataactgc tttaccagct gcattaactt tagtaatgat
     1501 cattttttgg taaactggaa ccatttcaaa tggaacttcg atacccatat caccacgagc
     1561 aatcataata ccatctgctg cttcgataat ctcatcaata ttatcgatac cttgttgatt
     1621 ttcaatttta gcaaacaact taacgtgtcc attgccagtt tcttcacaaa tagcacgaac
     1681 ttcattaaca tctttagcag tacgtacaaa tgagatagca ataaagttaa gtccttgctc
     1741 aagtccaaaa cggatatcag cattatcgcg ttctgcaagt gctgggaaag gaattttagt
     1801 ataagggatg tttacacctt tttgtttacc aataaggcca tcattctcaa caactacttc
     1861 aaattcacga gtgtctttat cttttgcaaa cacagtaaga cctagtttac catcatcaac
     1921 aaggatttgc ttaccaactt caacgtcatc aaagatgtca agtccaccag caacattcaa
     1981 tgcaatcact tctggagttg atttgatacc ttgcttagta gcaacacgta attttgtacc
     2041 tgttgtatat gaatggaaat ctgcaccatc ttcaaaaagt tctgtacgaa tttcaggtcc
     2101 tttagtatca aggaggaagc caactttttg tcctgcaatc tcttctgctt tacgaacagt
     2161 agccatacga gctccttgct cagcatgatc tccatgtgag aagttgaaac ggaaaacgtt
     2221 agcaccttct ttaatcaatt gagcaatttt ttctgctgaa gcttctacgt caaggctttc
     2281 accccagtat ccagactcac caaacttctt accaccacgg aattctaccg caggaccaag
     2341 tgttgcaacg atttttacgc gtttattcat aataatttat gagctccttt ttatttaaag
     2401 tgtcaaattg acaaattctt atttactact ttggttggca aggtcacgat taagtgctgc
     2461 caagcgaaga tccgcttcat gcggattatt aacaacgatt tttccttcat cagtcaagct
     2521 gaacaaagca ccttcttctg ctaaacctaa aattggactt tcaaccattt cttcgttgtg
     2581 gacaccaacg gctaaaccac cacgaccttc tttcaacaat tgaacagcgt acgctcccat
     2641 acgagatgct aagacacgat cacgagccgt cggactacca ccacggagca gatgtcctaa
     2701 attcgtcaca cgaagatcgc tatcgtctcc tgctgctttc attgtttttg caaactcatc
     2761 accactcata acaccttctg caagaacgat gatttggtga tgtttaccag ctgcatagcc
     2821 agctctaaca tttgagacaa cttcatcaat attgaactct tcttcaggaa caataatttg
     2881 atctgcacct gcagcgatac ctgaccaaag agcgatatct cctgcatttc ttcccataac
     2941 ctcaacaaca aaagtacggt tatgacttgc tgatgtatca cgaagacggt caagattctc
     3001 aactgctgtc gcaactgctg tgtcaaaacc aatagtatag tcagtgccaa cgatatcgtt
     3061 atcaattgta cccggcaaac caacagctgg gaaaccgtgc tcagttagac gcatagcacc
     3121 atgataagaa ccatcaccac cgataactac tacaccttca ataccgtgtt ttttaagctg
     3181 ttcaatccct ttaagctgac cttcaagttc agcaaattca ggataacggg ctgaacgtaa
     3241 aaacgttcct ccacggttga tagtatcccc aacagaatta gcatccaaag ggaaaatatc
     3301 ccctgtcacc ataccatagt aaccttggtt gatgccgtaa acttccatac cttcagaaat
     3361 tgctttacga acaactgcac ggatagcagc gttcatacca ggggcgtcac caccactagt
     3421 taaaacagca atacgtttca tttaattggt actcctttta ttaatttaac gatttaatta
     3481 tagcatatta atttgtaaat ttctgcactt tatatgcaat tttattgata aaccgttttc
     3541 acaacaaatt cagataaatc aagggctaag ccactatctt ttctaaccaa atgtactttg
     3601 ctttggatag tttctttagt tgtttcataa tgcaaataga caggaatatg ccctttatat
     3661 ttggacaata tttccgatat ttttttgtcg ttatcatgat tttttagaag tatccaaaaa
     3721 cgttcctccg ttgcttcttg aacaccgttt aaaaccatct gtaatcgctc atctcgcttc
     3781 tgaatttttc catgcaagta ataatatttc ccttcttgta aatcatctct tacaatattg
     3841 aaagtttcag gaaaaacagt aatatccatt tttgaattaa tatccataac agtcaagaaa
     3901 gccatttgtt gtccagaact ttttgtccta atgattttga cctgctttag ctgaactaaa
     3961 atagtagctt cgctattttc ttggacttga ctaattggta taaacggacg gctagctttt
     4021 tgtgaaatcc ccactaaagg gtgcggactg attcctactc ctaagatatc ttgttccatt
     4081 tcatatttct cagaattaga gaaatcttct acttctaacc aatgatatga ggtatctgca
     4141 aataaactac ctagttcatt tacaaatgta aataaagaat caagattaga ggtgattttc
     4201 cgtctattgt tatcaaattc gtcaaatgct ccaatagcaa ttagcggaga aataaaacca
     4261 cttttttgaa actgctgtgg cagtctcatt agaaaatctt caatactctg aaatggttga
     4321 tttttagaaa tccaataagc caagtcgtta ggaacagcct taatattttt taaacctaaa
     4381 taaattttct ttttatagat gcggtctctg tatgaaatag tattaattga aggtttttca
     4441 ataacaaaac caaaatcaat agcatcgatt agataatcac tatttgaata atttatcatg
     4501 atgtcataaa agacatcgga ataatgggct ttgaaatagg ctaattgaaa agctaaagcc
     4561 gaataagcaa aggcatggct gcgattaaac ccataacctg caaatttagc catgcgttca
     4621 aatataagtc gagcatcttc ttgacgatgt cccttagata ctgcaccctc taagaaggat
     4681 tgagacatct tttgcatctc cttagaattt ttcttagaca tagcacgtct gagcaaatca
     4741 gacttgccta gcgtaaaacc tgcgtaggtc tgggcaatct gcataacctg ttcttgatag
     4801 agcattatgc cataggtagg ttgtaaaatt ggagctatga caggatctac taaatcgatt
     4861 ttttctttac caaaacgacg gtttataaaa ttctcagtat aatcacttgc accaggccta
     4921 ttcaagctgg tagtagctac cacatcttca aaacactctg gcctaatacg tcgcaacaaa
     4981 ttaatagctc cactttgctc aaattgaaaa atcccctttg tttgaccagc cgcaaatagt
     5041 tttagagtct ctttatcttc taaatcaata gcttctaatt gtatagagat accgtagtcc
     5101 ttatcgactt tctccttcat tttctgaaca aatgtcaaat ttcgaagacc tagaaagtcc
     5161 atttttagaa gaccattagc ctcaacagaa ctagcatcat attgcgttat catcatatcc
     5221 tcaccgtttt ttaaaggaat atgatctgtt aacaaatcgt cactcattac taccccagcg
     5281 gcatgaatag aagtctgtct aggatttcct tcaatacgct tagcaatatc ataggctttt
     5341 tggtattcaa ttttactatc tattatttgt ctgaaagcta agttgcggtt ataaacactg
     5401 gtcaaattat ctctgaaatg aattttttta gttatgtttg ttaactcata ctctgatgca
     5461 ccaaagcgtt taaaaacatc tcgaatagcc tgtttcgcac caaaagttga gaaggttaca
     5521 atctgtgcag aatgcattga accatatcga ttacgtacat agcgcaagaa ttctccacga
     5581 taaatatccg gaagatcaat atcaatatct ggcatcgaat aacgctcttc atttaaaaaa
     5641 cgttcaaaaa ttaaattatg tttgacagga tcgataccgg taatatttaa agcatatgag
     5701 actaaacttc ccgctgctga accacgtccc attcccatat agtagccctt actacgacca
     5761 aatcttaata aatcccacac aataaggaaa tagtcatcaa agcccatttt gtgaatgatt
     5821 gttaattctt tttcgagacg ctcttgataa gcatcgctcc ataaaccttt actttttaag
     5881 ttattttgag ttaaggttct taattcttct ccagcaggaa tttctctatt aaaaataggt
     5941 aacttaaagt cagaatcaaa gtgataaaca acattctcta ccaaagattc taggttagct
     6001 aaagcttcag gaaaacgttt cttgaatgct agagtcattt cttgacaagg ttttaattct
     6061 tgatttttgc tcgcatattg tgcatctttt aacaggacat tatctcttat agcatgaagc
     6121 atctgtaatg tctcaatatc atttttttga aaatatcgaa cagtatctat ggctataata
     6181 ggtttggtat atggaatctg cggagaggtc aaattaacac caataacata atcaaagggt
     6241 aaattgagct gatcaagagc atcttgatag ggaagaatca attgcacccc ttcaagatgt
     6301 tctgtcaata aatttagaga catcttctca gacatttgag ccgttgacaa cttcaagaga
     6361 ttcttataac caactgtatt tatcgcaata cagtaaactt ctattgatct attttcaacc
     6421 actatatcaa agctaaatcc aactattggt cttaggtttg ccttttgcgc ctcttgaata
     6481 aaatgatatg ctgcataaag attactgtgg tccaagatac caatagtttg atatcctaat
     6541 gatttcgatt tagagacata tgtctttaaa tcaaccaagc tatccataaa agaataaacg
     6601 gttcttgtat caagttgtgc aaacatagta gccccttttt acgatttttt actattatac
     6661 tatgaaacaa taaacgatta aagtctcaat gataattcct tgacatttca aatatttttt
     6721 tgtactattt ttatagtaca aaaagtacaa gattatgaaa ggagttatct atggcttggg
     6781 aattcaatga aaaatcacct atttattcac aaattgctga acatataaag atgcaaattg
     6841 ttagtcaaga aataaaaagt ggtgaccaat tgcctaccgt gagagagtta gctcaagaag
     6901 ctggtgttaa cccaaatacc atgcaacgtg ccttcactga attggaacgt gaaggaatgg
     6961 tattctcaca acgtacttcc ggacgctttg ttacagaaga caacttacta attggtaaaa
     7021 ttcgtcaaca agttgcaaaa gcagagttag caacttttgt caacaacatg aaaaaaatag
     7081 gatataaatt agatgaaatt acggttgctc tcgatcattt tataaaggag attaattaaa
     7141 tgacacagtt attacaactt catcatgtta caaagaaata tcacaaacat attgctgtta
     7201 atgatgtgac tgtaagcatt ccaacaggaa agattatagg tttattaggt ccaaatggta
     7261 gcggaaaaac aacaatcatc aaaatgataa atggattact tcagccagat aagggagata
     7321 ttgttatcga tggttacaga ccgtctgttg aaaccaaaaa aattatttca tatctccctg
     7381 atacttcata tttacaagaa aatatgaaaa tcaaagacgt cgttacatta tttgaagatt
     7441 tctataacga ttttaattca aaggtagctt atcaactatt tgaggatctt aacctaaatc
     7501 ctaaggaacg tctaaaaaac ctatcaaaag ggaacaagga gaaagttcaa ctaatacttg
     7561 ttatgagtcg taaagctagg ctctacatcc ttgatgaacc tattggtggt gtagaccctg
     7621 cagcgcgtga ctacatttta aaaacaatca ttagcaacta ctctaatgat gcttctgttt
     7681 taatttcgac tcacttaatt tctgacattg aacctatcct tgatgaagtt atcttcctta
     7741 aagaaggaga aattgaccta caaggcaatg ctgatgactt aagagaagag cacaactgtt
     7801 cgattgatgc tctctttcgt gaaagattca agaaataaag gagattgata tgtttggaaa
     7861 attattaaaa tatgaattaa aatcagttgg taagtggtat ctaacactca acgctgcggt
     7921 tttattagta tctattattt taggactggt attaaaggca cttggcggca atttttctac
     7981 tgatactaat agtactagcg cccaaatatt cactataata ctcgttttat tgttagcgat
     8041 ggttatttca ggatctctgt tatccaccct tgctattatt atcaaacgtt tttatagcaa
     8101 tatcttcggc cgtcaaggat atctaacctt aacgctccca gtaactacga atcagattat
     8161 ttgttcaaag ctactagctt cgcttctttg gagtattttt aatattttta ttgtcattat
     8221 tggaatcata ttggtcatat tacctttagt aggtattggt cagttcgttg tcgcttttcc
     8281 agaaatctat aaaattattt catcttcgaa cgctccactt tttatcgcct atttctttct
     8341 atcgtacgtt gcagggacat tactgattta tctctctatc gctgtagggc aattatttac
     8401 aaacaaacgt gtattgatgg gaattgtttc ttattttgga atatcactct taattacttt
     8461 cttaacatta ataatagata gtatttttca tattgattta ttcaactctc atgcaaatgc
     8521 aacattctct caacctgtat tgctctacaa tatacttgtt agcattgttg aaattgctat
     8581 attctatatg ttgacacact ctattattaa gtacaaactt aatatacaat aaaaagattg
     8641 gagatatatc tccaaccttt ttttattttt aattcaaaaa atttttcaaa atatcaccgc
     8701 cataaatcca caaatagcta taaccgatga tagaaatagg tttcgttatc ataatgattg
     8761 tcacaaagcg cttaatagac atattagata gaccggtaat catcaccatg atgtccgctg
     8821 gtgaaatggg agatgccata caaaagataa aaaacttttc atatccggat gtttctagct
     8881 tagactcata tttatcaaat gtcttctgat ccataaatag caaaacgaat tttcgtccat
     8941 aaaatttgac taaccaaaaa aggatgacac ttccgatgat aatcccaata tagttataaa
     9001 taaagcctaa ggtaggacca aaaattaaga aacctgctac tgttgtaaca cctccaggaa
     9061 ttactggaaa aacaatctgt acaatttgag ccacaataaa aatcattgga ccccaaaatt
     9121 catacttgtg tactaggtct tttaattcat tgctatcatt caaaatacca agtttgtaca
     9181 aataaacaac taagacaagg gtcgctatta acgctaagac acccaaagcc ttaatgacct
     9241 tctgccaaaa gcggtaacgt tttgataatt tcatattcat gctttaatca taacacaact
     9301 gttaaatttt tgctatttta aatagtattt gttatactat attggtaagt ttttatgctt
     9361 tattctactt ttggggtcgt tacggattcg acaggcatta tgaggtatat tttgcgactc
     9421 atcggcagat gtaaaatgcc agttaaatat aactgcaaaa aatacaaatt cttacgcatt
     9481 agctgcctaa aaaacagcct gcgtgatctt cacaagattg tttgcgtttt gctagaaggt
     9541 cttatttatc agcaaactac gtttggctac tgtctagtta gttaaaaaga gatttataga
     9601 ctcgctatgt gagggcttga gttatgtgtc atcacctagt taaatcaata cataacctat
     9661 agttgtagac aaatatatta gcagatgttt ggacgtgggt tcgactccca ccggctccat
     9721 atcattttat cataacttac tatacatact taaaaccttg ttattacaag gtttttattt
     9781 tgtttctttg ctcatacatt gcccctatct taggaaaaaa atacttggaa cagactctaa
     9841 cattgaatta ggccacctca ttagctcact ctttgtcaat atccgagaat aagttcttta
     9901 tcattctaat ttatagaatt tattaaaaaa gataaggttt ttagaggaaa aaatgttata
     9961 atctatttag cctacgattg gaggctagtt atggctagta ttttaaaaaa acatttagag
    10021 aaacgtttag agcttaaaaa tgatcctatg aggacagata tgagtactgg tgccagttac
    10081 cctaaatatg gattctcggt aggaaagtac acatatggat atcaacaatt cttttacgaa
    10141 ggcgttaact taaaagaaat tggagcattt tgctcaatag cccagaacgt tactattact
    10201 ggtatgaatc atccaaccga tcacattacc actaaccctt ttatttacta taagtcaagg
    10261 ggatttataa atgaggatag ggcggactta atagatgaaa agaaaaatgg aaaagttatt
    10321 attggcaacg atgtctggat aggaactaat gtcactatac ttccatctgt tacaataggt
    10381 aatggagcaa ttattgaagc aggttcagtt attaccaaag acattcctga ctatgctgtt
    10441 gtcgctggaa cacctgctaa aattatcaaa tatcgctttt cagaggaaga aataacatta
    10501 ttaaatgcat ctcagtggtg gaattggtca gatgaagcta ttaaggaaca tattagcgaa
    10561 ttttcagata agagggaatt cttcaatact ctaaaaagta tttctgaaaa caaaaatcat
    10621 aaactctaga atgacttcat caaatagcac actaaaat
//
LOCUS       DATROQ010000064        10637 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.065, whole genome shotgun sequence.
ACCESSION   DATROQ010000064 DATROQ010000000
VERSION     DATROQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10637
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.065"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..1175
                     /locus_tag="VBC94_001469"
     CDS             <1..1175
                     /locus_tag="VBC94_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6979730.1"
                     /translation="YKSFKDIFPFALIFSWIEILQKLVISDDSFLAQTTKSSIYKNEW
                     LYTVSNTLYDLSHLLIYFMASYFVASLVWRGLSNQTKESFSASLLGFATVWLLLTSVV
                     PGTDYHYPSQPIWLMLALISYLFIFLTRLLQNRHHIFQYSMLAILLLISSILSHILYS
                     YPNIDLEYLIQNHFSQWLGDGPHQLIPIMLWSILAIFLTLSGLFVPDILFRPYSYFSV
                     VSENLNAALSQHTDKIPYLYTFYTVKNSFAMFGGIGILLSLFLAVLYESRKLQSKNYY
                     KLTLLTLTPLIFDQNLPFLVGLPVILQPILFIPMVLTTIFAEAFGALMLYLKFVDPAV
                     YTVPSGTPSLLFGFLASNGDWRYLPVTAIILVVGFFIYRPFVKIAFAKEEQYEKIT"
     gene            1159..2001
                     /locus_tag="VBC94_001470"
     CDS             1159..2001
                     /locus_tag="VBC94_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6979731.1"
                     /translation="MKKLLRWLPPVLFIIILIGMTILGKSYINKVTAHKIKLYNSRMT
                     PTILIPGSSATQERFNSMLAQLNQMGEKHSVLKLTVKKDNSIIYNGQISGNDHKPYIV
                     IGFENNEDGYSNIKKQTKWLQIAMNDLQKKYKFKRFNAIGHSNGGLSWTIFLEDYYDS
                     DEFDMKSLLTMGTPFNFEESNTSNHTQMLKDLISNKGNIPSSLMVYNLAGTNSYDGDK
                     IVPFASVETGKYIFQETAKHYTQLTVTGNNATHSDLPDNPEVIQYVAEKILKNEKGKL
                     PKPH"
     gene            2078..2905
                     /locus_tag="VBC94_001471"
     CDS             2078..2905
                     /locus_tag="VBC94_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEO6979732.1"
                     /translation="MKLISWNIDSLNAALTSESTRALMSRQVIDTLVAEDADIIAIQE
                     TKLSAKGPTKKHLEVLETYFPEYDLVWRSSVEPARKGYAGTMFLYRKGLNPIVSFPEI
                     DAPTTMDNEGRIITLELENCYITQVYTPNAGDGLKRLADRQIWDIKYAEYLATLDSQK
                     PVLATGDYNVAHKEIDLANPSSNRRSAGFTAEERQGFTNLLAKGFTDTFRYLHGDVPN
                     VYSWWAQRSRTSKINNTGWRIDYWLTSNRVADKITKSEMIHSGDRQDHTPIILEIEL"
     gene            complement(2945..3301)
                     /locus_tag="VBC94_001472"
     CDS             complement(2945..3301)
                     /locus_tag="VBC94_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEO6979733.1"
                     /translation="MYTFYEYPKCTTCRSAKKELTELGLTFEAIDIKSNPPKASLLKE
                     LLENSPYDLKKFFNTSGNSYRELGLKDKFDDLTLDQALDLLASDGMLIKRPLLVKDNK
                     ILQIGYRTKYKDLNLV"
     gene            complement(3303..3779)
                     /locus_tag="VBC94_001473"
     CDS             complement(3303..3779)
                     /locus_tag="VBC94_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEO6979734.1"
                     /translation="MLYQEFYQSPLGEIRLLADNLGLSGLYFVGQKYDMLAVNQEEIV
                     NMSNSYTLLGKKWLDAYFSQQNLPSIPLSLRGTAFQTRVWQELQKIPFGDTKTYGELA
                     KELNCQSAQAVGGAIGKNPISLIIPCHRVLGRYGQLTGYAGGLERKSWLLEYEKEK"
     gene            complement(3834..4984)
                     /locus_tag="VBC94_001474"
                     /pseudo
     CDS             complement(3834..4984)
                     /locus_tag="VBC94_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
     gene            complement(5057..5605)
                     /locus_tag="VBC94_001475"
     CDS             complement(5057..5605)
                     /locus_tag="VBC94_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO6979735.1"
                     /translation="MQIRLAFPNEIDQIMLLIEEARAEIAKTGSDQWQKEDGYPNRND
                     IIDDILNGYAWVGIEDGMLATYAAVIDGHEEVYDAIYEGKWLHDNHRYLTFHRIAISN
                     QFRGRGLAQTFLQGLIEGHKGPDFRCDTHEKNVTMQHILNKLGYQYCGKVPLDGVRLA
                     YQKIKEKGETSIYREIDERNPM"
     gene            complement(5673..6764)
                     /gene="serC"
                     /locus_tag="VBC94_001476"
     CDS             complement(5673..6764)
                     /gene="serC"
                     /locus_tag="VBC94_001476"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEO6979736.1"
                     /translation="MIIYNFSAGPAVLPKPVLVKAQSELLNYQGSSMSVLEVSHRSKE
                     FDDIIKGAERYLRDLMGIPDNYKVIFLQGGASLQFSMIPLNITRGRKAYYHVAGSWGK
                     KAYTEAVKLSKTIPFEPILLASSEESVYDYIPEFDEKEIDPKAAYVHVTTNNTIEGTS
                     LYDIPKTNGVPVIADMSSNILAVKYKVEDFAMIYAGAQKNIGPAGVTVVIIREDMINE
                     EPTLSSMLDYKVQSDAGSLYNTPPAYSIYIAKLVFEWVKSLGGVDAMEKANREKSGLL
                     YDYIDSSEFYSNPVRDKKSRSLCNIPFITINKDLDEKFVKEATERGFKNIKGHRSVGG
                     MRASLYNAFPKQGVIELIDFMKTFEAENT"
     gene            6897..7532
                     /locus_tag="VBC94_001477"
     CDS             6897..7532
                     /locus_tag="VBC94_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEO6979737.1"
                     /translation="MILREFCAENLTDLTRLDKAIISRVELCDNLAVGGTTPSYGVIK
                     EANQYLHEKGISVAVMIRPRGGNFVYNDLELRIMEEDILRAVELESDALVLGILTSNN
                     HIDTEAIEQLLPATQGLPLVFHMAFDVIPKSDQKKSIDQLVALGFTRILLHGSSNGEP
                     IIENIKHIKALVEYANNRIEIMVGGGVTAENYQYICQETGVKQAHGTRITQ"
     gene            complement(7802..8671)
                     /gene="rsmI"
                     /locus_tag="VBC94_001478"
     CDS             complement(7802..8671)
                     /gene="rsmI"
                     /locus_tag="VBC94_001478"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEO6979738.1"
                     /translation="MEMQVQKSFKSNIHYGTLYLVPTPIGNLDDMTFRAIRILREVDF
                     ICAEDTRNTGLLLKHFDITTKQISFHEHNAYDKISGLIDLLKEGKSLAQVSDAGMPSI
                     SDPGHDLVKAAIEGDIPVVSIPGASAGITALIASGLAPQPHIFYGFLPRKKGQQITFF
                     ETKQDYPETQIFYESPFRVSDTLKHMKEIYGDRQVVLVRELTKLYEEYQRGTISQLLE
                     HIEKVPLKGECLIIVDGKRDTERVKDSSQQDPLVLVKEYIANGDKTNQAIKKVAKEFN
                     LNRQELYASFHDL"
     gene            complement(8671..8997)
                     /gene="yabA"
                     /locus_tag="VBC94_001479"
     CDS             complement(8671..8997)
                     /gene="yabA"
                     /locus_tag="VBC94_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEO6979739.1"
                     /translation="MDKKDLFDAFDDFSQNLLVGLSEIETMKKQIQKLLEENTVLRIE
                     NGKLRERLSVIEAETETAVKNSKQGRELLEGIYNDGFHICNTFYGQRRENDEECAFCI
                     ELLYRD"
     gene            complement(9027..9890)
                     /locus_tag="VBC94_001480"
     CDS             complement(9027..9890)
                     /locus_tag="VBC94_001480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEO6979740.1"
                     /translation="MDLKRTQPKLLEKFNTILQSDRMSHAYLFSGNFASLDMALYLAQ
                     SQFCEKRQSGLPCQECRACRLIANGEFSDVKIIEPQGQLIKIETIKELTKDFSRSGFE
                     GKSQVFIIKYCEKMHVNAANSLLKFIEEPQSSSYVILLTNDENNVLPTIKSRTQIFRF
                     PKQLDMLVHQAEQAGLLKSQASLLAQVADDPKHLEILLTNKKLLDYLNLSQQFVTTLA
                     KDRQTAYLEVSRLTSQVVDKNDQAFVFQWLTIMLAKEGQLYDLENTYRAQQMWKSNVS
                     FQNSLEYMVLS"
     gene            complement(9910..10545)
                     /locus_tag="VBC94_001481"
     CDS             complement(9910..10545)
                     /locus_tag="VBC94_001481"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEO6979741.1"
                     /translation="MKKGLMISFEGPDGAGKTTVLEAVLPLLREKLSQDILTTREPGG
                     VTISEEIRHIILDVKHTQMDKKTELLLYMAARRQHLVEKVLPALEEGKIVLMDRFIDS
                     SVAYQGSGRGLDKSHIKWLNDYATDSHKPDLTLYFDVPSEVGLERIQKSVQREVNRLD
                     LEQLDMHQRVRQGYLELADSEPNRIVTIDASQQLDEVIAETFSIILDRINQ"
BASE COUNT         3324 a         2010 c         1677 g         3626 t
ORIGIN      
        1 tttataaaag ttttaaagat atctttcctt ttgctctcat tttttcttgg attgaaatcc
       61 ttcaaaaact cgttatatca gatgattctt ttttagctca aacaacaaag tccagtattt
      121 ataaaaacga gtggttatat acggtttcca atactcttta tgatttatct catttactaa
      181 tctactttat ggctagttat tttgtggcta gtttagtatg gagaggctta tctaaccaaa
      241 ctaaagaaag tttttctgca agtttacttg gctttgccac tgtttggttg cttctaactt
      301 cagttgttcc aggtacagat tatcactatc ctagccaacc catttggctt atgctggctt
      361 tgatttcgta tctttttatc tttttaactc gactgcttca aaatcgacat catattttcc
      421 agtatagtat gttagctata ctactattaa tctcttctat tttgtcacat atcttatata
      481 gttatcctaa tattgattta gaatacctta tacaaaatca cttttctcaa tggctgggtg
      541 atgggccaca tcaattaatt cctattatgc tttggtctat cttagctatt tttctaacct
      601 tatcaggtct ttttgtacca gatattttat ttcgtcctta ctcctatttt tcagtagtta
      661 gcgaaaattt aaatgctgcc ttgagccaac atacagataa aatcccttat ctgtatacct
      721 tttatactgt taaaaatagc tttgctatgt ttggcggtat tggaatccta ttatccttat
      781 ttttagcagt tttatatgaa agtagaaaac tacaatcgaa gaattactat aaattgacat
      841 tactgacctt aactcctctt atttttgatc aaaatttacc atttttagta ggtctacctg
      901 tcattttaca accaatatta ttcattccga tggtattaac aactatcttt gcagaggctt
      961 ttggagctct tatgctctac ctaaaatttg tcgaccctgc agtttatact gtcccaagtg
     1021 gtacacctag tctactattt ggctttcttg cttcaaatgg ggattggcgt taccttccag
     1081 tcactgcaat tattctagtt gttggcttct ttatttatcg tccgtttgtc aaaatcgctt
     1141 tcgctaagga ggagcaatat gaaaaaatta cttagatggc ttcctcctgt acttttcatt
     1201 attatcctta taggaatgac tatcttaggt aagtcctata tcaataaagt aacagctcac
     1261 aaaataaaac tctataactc tcgaatgact cctactattt taattccagg atccagtgct
     1321 actcaagaac gatttaacag catgttagca cagctcaacc aaatgggaga aaaacatagc
     1381 gttttaaagt taactgtcaa aaaagacaat agcattatct acaatggaca aattagcggc
     1441 aatgaccaca aaccctacat tgtcattgga tttgaaaata atgaagatgg ttatagtaac
     1501 atcaaaaaac aaacaaaatg gctacagatt gctatgaatg atcttcagaa gaaatataaa
     1561 tttaaacgtt ttaacgctat cggtcattca aatggtggct tatcatggac tattttccta
     1621 gaagattatt acgactctga tgaatttgat atgaaatcat tgttaacaat gggaacacct
     1681 tttaactttg aagaaagtaa cacctcaaat catactcaaa tgcttaaaga tttaatcagt
     1741 aataaaggaa atattccatc aagtctcatg gtatacaatt tggcaggaac taattcatat
     1801 gatggtgata aaattgttcc atttgctagt gtggagactg gtaaatatat tttccaagaa
     1861 accgctaaac actataccca actaacagta actggtaata atgctacaca ttctgacttg
     1921 cctgataatc ctgaagttat ccaatatgtc gcagaaaaaa ttcttaaaaa tgagaaaggt
     1981 aaattaccaa aacctcacta aagctgtaga atagcaacgc ctctggtatt ttgatataat
     2041 atattgcgaa ttaaactaaa taaaaaagga tattattatg aaactcattt catggaatat
     2101 tgattccctc aatgcggcat taacaagtga atcaacacgt gctttaatgt cacgccaagt
     2161 tattgatact ttagttgctg aagatgccga catcattgcg attcaagaaa ctaaactgtc
     2221 tgccaaagga cctactaaaa aacatttgga agtacttgaa acttacttcc cagaatatga
     2281 tttggtttgg cgatcatcag tcgaacctgc tcgtaagggc tatgctggta caatgttctt
     2341 atatcgtaaa gggttaaatc ctattgttag cttccctgaa atcgatgctc ctactacaat
     2401 ggataatgaa ggtcgcatta ttactctcga attggaaaac tgctatatca cacaggtcta
     2461 tactccaaat gcaggtgatg gcctaaagcg cttagctgac cgtcaaatat gggatattaa
     2521 atatgctgag tatttagcaa cattggatag tcaaaaacct gttctagcaa caggtgacta
     2581 taacgtagcc cacaaggaaa tagacctagc taatcctagc agtaatcgtc gttctgctgg
     2641 attcacagct gaagagcgcc aaggctttac aaatctacta gccaaaggtt ttacagatac
     2701 ttttcgttac cttcatggcg atgttccaaa tgtttatagc tggtgggcac aacgaagtcg
     2761 tacatccaaa atcaacaata cagggtggag aattgattat tggctaacaa gtaaccgtgt
     2821 cgcagataaa attaccaagt cagaaatgat tcattctggt gatcgtcaag atcatacacc
     2881 aattattttg gagattgaac tataaaccaa aaaaagatga ttgagttata actcaatcat
     2941 ctttttatac taagttgagg tctttatact tagtacggta gccaatctga agaattttat
     3001 tatctttaac tagcaatgga cgcttaataa gcattccatc cgatgccaat aagtccaaag
     3061 cttggtcaag agttaaatca tcaaatttat cttttaagcc taattctctg taagaatttc
     3121 ctgaagtatt gaaaaacttt ttaaggtcat aaggcgaatt ctctaacaat tcttttaata
     3181 acgaagcttt gggaggattg ctttttatat caatcgcttc aaaagtcaag ccgagttcag
     3241 ttaactcttt ttttgctgaa cgacaagtgg tacatttggg gtattcataa aacgtataca
     3301 tattattttt ccttttcgta ttctaataac caagattttc tttctaagcc accagcataa
     3361 ccagttaatt gaccatatct tcctaaaaca cggtgacaag gtataattag agaaatagga
     3421 tttttaccta ttgctcctcc aacagcctgt gcagattgac aatttagctc tttcgctaat
     3481 tctccataag ttttggtatc tccaaaaggg attttctgta attcctgcca tactctcgtc
     3541 tgaaatgctg tccctctcaa tgataatgga atactaggta aattttgctg tgaaaaataa
     3601 gcatctaacc acttcttacc aagtaaagta taggaatttg acatattaac aatttcctct
     3661 tggttgactg ctagcatatc atatttttgc ccaacaaaat aaagccctga taaacctaga
     3721 ttgtctgcta gaaggcgaat ttctccaaga ggggactgat agaattcttg atataacata
     3781 tgataagcat aacttaaaaa gccatgaaaa gcaattcatg actttttagt tctttattgt
     3841 tgttttgcaa ttaaacgtac acggacgata ttctcatcac cttcaaattc ttcaattaaa
     3901 gtagaaatct tgttattatc cgtttcatct aaatctatta gtgtgtaggc gtagtcaccc
     3961 tttgaacggt taataatgtt atcaatattt atacctaaca ctgatacagc agttgaaatt
     4021 ttagccacaa tgttaggaac attcttgtta attaaagtaa tacgaaatgg tgcggtttga
     4081 atttgatgta cattggggaa attaactgaa ttagtgattt ctcctgtctc cataaaacaa
     4141 cgaatggttt gacttgccat aatagcacaa tttagctctg cctcatctgt tgatccgcct
     4201 acatgaggaa agactgtaat tcctttttgg tttaataatt ctttgtctcc aaaatcagta
     4261 atatagcgct tgacaacacc agtttctatc gcttcaaata gctcttggtt attgactaat
     4321 tctgcacgag caaagttgat aatcgtagtt ccttttttca tgattgaaaa agcttccgca
     4381 tcaaaagtat gcttagtttc atttgttaag ggaacatgaa ttgtgatata gtcacaagtt
     4441 tcaaaaatat ccttaatctc tttaaccctt tgaacatggc ttgaaatatt ccaagctgtt
     4501 tcaattgaaa tatagggatc ataaccaaga actgtcattc ctaagcgtct agcacattcg
     4561 caattctagc tccaatggca ccaaggccga taactcccaa tttttttcct gcaatttcat
     4621 taccagcaaa agctttcttt cctgcttcaa tttgtttggg aatatctgtt ccagttagag
     4681 tattaaccca tcggttagct cctaaataat cacgagctga aagtaataag gcagcaatga
     4741 ccgcttcttt tacagcatta gcatttgcac ctggggtatt aaacacgact attccctgtg
     4801 cacttgcctc ttcaatagga atattatttg ttcctgcacc cgccctagca atagctttga
     4861 ggttacttgg gaaatcctga ttatgcaaat tttgactacg aataatatag gcatcaggat
     4921 tttctgacat atccccatca atctggaaac gattccctaa ctcttgtaaa ccaatttgat
     4981 taatattatt aaatgttttg acactaaggt tatctcttta gtcatcattg accaaaataa
     5041 cccctttctt tttttcttac atgggatttc tttcatcaat ctcacgatag atgctcgttt
     5101 ctcccttttc ttttatcttc tgataagcca atcgtacccc atctagaggt actttaccac
     5161 aatattgata gcctaattta tttagaatat gctgcatagt gacatttttt tcatgagtat
     5221 cgcaacgaaa atcaggtcct ttatgacctt caattaaccc ttgaaggaat gtctgtgcta
     5281 gtcctctacc acgaaattga tttgagatcg caatacgatg aaatgtaaga tagcgatggt
     5341 tatcatggag ccattttccc tcataaatag catcataaac ctcttcatga ccatcaatga
     5401 cagccgcata agtcgctagc atcccatctt caataccaac ccaagcataa ccgttcaaga
     5461 tatcatcaat aatatcgtta cgattagggt atccatcttc tttttgccat tgatcacttc
     5521 ctgttttagc aatttcggca cgcgcttcct caataagaag cataatttgg tcaatttcat
     5581 ttggaaaagc taatctgatt tgcattatat tacccacttt tatatattat agactataat
     5641 aacggtttaa ttttctttcg tgataacatt tactatgtat tttctgcttc aaatgttttc
     5701 ataaaatcaa ttaattcgat gacaccttgt ttaggaaagg cattataaag gctagctctc
     5761 ataccaccta ccgaacgatg tcctttaata tttttaaatc cacgttctgt cgcttccttt
     5821 acaaattttt catctaaatc tttattaata gttatgaaag gaatgttaca tagagaacga
     5881 ctctttttat ctctgacagg attactataa aattctgagg aatcaatata atcatagaga
     5941 agtcctgatt tttcacggtt agctttttcc atagcatcaa caccacctag acttttcacc
     6001 cattcaaaga ctaatttagc aatgtaaata ctgtaagcag gcggcgtatt ataaagagaa
     6061 ccagcatctg actgaacctt ataatccaac attgatgata aagttggttc ctcattaatc
     6121 atatcttcac ggataataac taccgttaca ccagcaggtc caatattttt ttgagctcct
     6181 gcataaatca ttgcaaaatc ttctacttta tattttacag ctaatatatt agaagacata
     6241 tccgcaataa caggcactcc attagtttta ggaatatcat aaagcgatgt tccttcaatg
     6301 gtattatttg ttgtcacatg aacataggca gctttaggat caatctcttt ttcatcaaac
     6361 tctggaatat aatcataaac tgattcttct gatgaagcta atagaattgg ttcaaaagga
     6421 attgtttttg atagttttac agcctctgta taagcttttt tcccccacga accagcaaca
     6481 tgatagtaag ctttacgccc tctggtaata ttcaagggaa tcatactaaa ctgtaaggat
     6541 gcaccacctt gtaaaaagat tactttataa ttatccggaa ttcccatcaa atctcttaag
     6601 taacgctcag cacctttgat aatatcatca aactctttgg agcggtgcga tacctccaaa
     6661 acactcatac tagacccttg ataattaagg agttcagatt gggcttttac aagcactggt
     6721 tttggtaaga ctgcaggacc tgcagaaaaa ttataaatta tcatataaca cctccatata
     6781 attgtgtaag attatagcac agtattttga atattgtaaa gatttttttg attaattttg
     6841 ctttttattc gtgatataat ggtaaaggcg aacaatcaag tttaaaggag taatttatga
     6901 ttttgaggga attttgtgca gagaatttaa cagacttaac cagattagat aaagctatta
     6961 tttcacgtgt cgagctttgt gataaccttg ctgtcggagg cactactcct agctacggtg
     7021 ttatcaaaga agctaatcaa tatttacatg aaaaaggtat tagtgtcgct gttatgattc
     7081 gtccccgtgg aggaaatttt gtatacaatg atttagaatt acgtattatg gaagaggata
     7141 tcctacgtgc tgttgagtta gaatcagatg ctcttgtttt gggaatatta acttcaaata
     7201 atcatattga tactgaagcc attgaacaac ttctacctgc tacccaaggt ttgccacttg
     7261 tttttcatat ggcatttgat gttatcccga aatctgacca aaaaaaatca atagatcaat
     7321 tagttgcttt aggctttaca aggattctcc tacatggttc atctaatggc gaaccaatta
     7381 tagaaaatat caaacatatc aaagcattag ttgaatatgc taataatcgt attgaaatta
     7441 tggttggcgg cggtgtgact gctgaaaact atcaatatat ttgccaagaa actggggtta
     7501 aacaagccca tggtactaga ataactcaat aaaaaattcg aaacagggct ataagtttct
     7561 ttagtatatt tactactaag ttctctcatg ttttgtaaaa cctgaaagaa ccggaataag
     7621 agcataccat cttttataac ctactctcag aaataaaatc caatttgcaa tggtattcaa
     7681 aagagctgaa attaaaaatt ttaaggaaat tggaagtatc ttacttcttt attccaatag
     7741 ctaaatcgga gattataaaa ggttgccaaa tttttctttg gcaacctttt tatattatta
     7801 cttataaatc atggaaacta gcatagagtt cttgtctatt gagattaaat tcttttgcta
     7861 ctttttttat cgcttgatta gttttatcac cattagcgat atattctttt actaatacta
     7921 gtggatcttg ttggctactg tctttcactc gctcggtatc tctcttacca tcaacaataa
     7981 ttaagcattc acctttgaga gggacctttt caatatgctc taaaagttga ctaatggttc
     8041 ctctttgata ctcttcatag agtttcgtca attcgcgtac taaaacaact tggcgatctc
     8101 cgtaaatctc tttcatgtgt tttagcgtat cagagactcg aaacggtgac tcataaaaga
     8161 tttgtgtttc agggtaatct tgctttgttt caaagaaagt tatttgttga cctttcttac
     8221 gtggtaagaa gccataaaaa atatgaggtt gtggagctaa acctgaagcg atgagagcag
     8281 taataccagc gctagctcct ggtatagata caactgggat atccccttca atagcagcct
     8341 tgacaaggtc atgtcctggg tcagaaatag agggcattcc tgcatcagat acttgggcta
     8401 aagatttccc ttcttttaac aaatcaatta acccagagat tttatcgtaa gcattgtgtt
     8461 cgtgaaaact aatttgttta gtagtaatat caaagtgctt gagtaaaagt cccgtatttc
     8521 gtgtatcctc tgcacaaata aaatcaactt ctcttaaaat cctaatggca cgaaaagtca
     8581 tatcatctag attaccaatt ggagttggga ctagatagag tgttccgtaa tgtatatttg
     8641 atttaaaact tttttgaact tgcatttcca tcaatctcta tataataatt caatacaaaa
     8701 agcacattct tcgtcatttt cacgacgttg accatagaaa gtattacaaa tgtgaaagcc
     8761 atcattgtaa atgccttcga gtaattctct tccttgttta gaatttttaa ctgcagtttc
     8821 tgtttctgct tcaataacgc tcagacgctc acgaagttta ccattttcaa tgcgtagaac
     8881 agtattctcc tccaataatt tttggatttg ttttttcata gtctcaattt cagacaaccc
     8941 aactaataaa ttttgtgaaa aatcatcaaa ggcatcaaac aagtcttttt tatccattca
     9001 ctcttcctct ctgcgcgtat tattttctaa gaaagcacca tatactctaa actattttga
     9061 aaactaacat tgctcttcca catttgctga gctctataag tattttctaa atcataaagt
     9121 tggccttctt tagccagcat tattgtcagc cattgaaaaa caaatgcctg atcattttta
     9181 tctacaactt gcgatgtcaa gcgagatact tctaaataag cagtttgcct atcttttgcc
     9241 aaagtcgtca caaactgttg acttaaattc aagtaatcta ataacttttt gttagtcaac
     9301 aatatttcta aatgtttagg atcgtctgct acttgagcta ataagctagc ttgtgatttt
     9361 agcaatcctg cttgttcagc ttgatgaacc aacatgtcca attgttttgg aaaacgaaaa
     9421 atttgcgttc tactcttaat tgttggcaaa acattgtttt catcattagt caataagatg
     9481 acataagatg aactttgagg ttcttcaata aatttcaaaa gactgttggc tgcattgaca
     9541 tgcatttttt cgcaatattt gatgataaag acctgtgatt tcccttcgaa acctgatctt
     9601 gaaaaatctt tagtcagctc ttttatagtc tctatcttaa taagttgccc ctgtggttct
     9661 atgattttaa catctgaaaa ttccccatta gcgattaaac gacaggctct acactcttga
     9721 catggtaaac cactctggcg cttttcacag aactgacttt gtgctaaata aagtgccata
     9781 tctagacttg caaaatttcc cgaaaaaaga taggcatgac tcatcctatc tgactgtaaa
     9841 atagtattaa acttttccag caatttgggt tgtgttcgct ttaaatccat gacctttgta
     9901 cttcctgatt cattggttga tacgatctaa aataatagag aatgtctcag ctattacttc
     9961 atctagctgt tgggaggcat caatcgtcac aatgcggttt ggctccgaat ccgccaattc
    10021 aaggtaacct tgacggacgc gttgatgcat atctaattgc tcgagatcta gacgatttac
    10081 ctccctttga acactttttt gaatacgctc taaacctact tcagaaggaa catcaaaata
    10141 taaagttaaa tcaggtttat gactatcagt cgcatagtca ttaagccatt ttatatgtga
    10201 cttatctaat cctcttcctg atccctgata ggcaacagaa ctatcaataa aacgatccat
    10261 taggactatt ttcccttctt ctagagctgg gagtaccttt tccactagat gttggcgtcg
    10321 agcagccatg taaagcaata gctctgtttt tttatccatt tgcgtgtgtt taacatctaa
    10381 aataatatga cgaatttctt ctgaaatagt tacgccacca ggctctctgg tggtgagaat
    10441 atcttgtgat aatttttctc tgagcaaggg taaaacagct tctaaaacgg ttgttttacc
    10501 cgcaccatct gggccctcaa aagaaatcat tagtcctttt ttcatggtac atcctttatt
    10561 tataattact actattctat caaaatttat caaaaaaaga cacccacaac ttgattgtgc
    10621 atgtctgata tttttaa
//
LOCUS       DATROQ010000065        10536 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.066, whole genome shotgun sequence.
ACCESSION   DATROQ010000065 DATROQ010000000
VERSION     DATROQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10536
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.066"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(27..1499)
                     /locus_tag="VBC94_001482"
     CDS             complement(27..1499)
                     /locus_tag="VBC94_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6979742.1"
                     /translation="MEKTKSFFGHPRGLSTLFFTEMWERFSYYGMRAILLYYMYYSVS
                     QGGLGMDKTVAASIMAVYGSLVYLSSVIGGFVSDRILGSRKTVLYGGILIMLGHIALA
                     TPFGQTALFISIALIILGTGLLKPNVSEMVGNLYGENDSRRDAGFSIFVFGINLGAFI
                     SPIVVGYLGQEVNFHLGFSLAAIGMFFGLLQYTLDGKKYLTEESLRPNDPLSPEEKSS
                     LYKKVGLILIGIVIVLILLHLMHMLTIEVIIDIFSIIAIAIPIIYFIKILSSKKISSV
                     ERSRVWAYIPLFIASILFWSIEEQGSVVLALFADEQTKLYLNFFGHHINFPSSYFQSM
                     NPLFIMLYVPFFAWLWAKWGSKQPSSPKKFAYGLFFAGASFLWMMLPGLLFGVNAKVS
                     PLWLTMSWAIVIVGEMLISPVGLSATSKLAPKAFQAQMMSIWFLSNAAAQAINAQIVK
                     LYTPDTQILYYGVVGGITVVFGFILLFYVPRIEKLMSGVK"
     gene            complement(1730..2566)
                     /locus_tag="VBC94_001483"
     CDS             complement(1730..2566)
                     /locus_tag="VBC94_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6979743.1"
                     /translation="MNKKKRLNLTFVYILLIVLSIMWLFPIVWVVLTSFRGEGSAFVN
                     YFIPKTWTLDNYAKLFTQNTFPFGQWFLNTLFVATCTCILSTLITVAMAYSLSRIKFK
                     HRNGFLKLALVLNMFPGFMSMIAVYYILKALNLDQTLTALIFVYSAGAALTFYIAKGF
                     FDTIPYSLDESAMIDGATRLDIFLKITLPLSKPIIVYTALIAFMGPWMDFIFAKVILG
                     DATSKYTVAIGLFSMLQQDTINQWFMSFTAGSVIIAIPITILFMFMQKYYVEGITGGS
                     VK"
     gene            complement(2566..3936)
                     /locus_tag="VBC94_001484"
     CDS             complement(2566..3936)
                     /locus_tag="VBC94_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6979744.1"
                     /translation="MNKKLISLDGMSLKEVFQKGDLATKLSFLIMGLANLKNKQIVKG
                     LLFLISEILFLITFVYQVIPAVKGLISLGTQEQGMTTKTVDGIKIQVATQGDNSMLML
                     IFGLASLIFCCVFAYIYWSNIKSAAHLLTLKEEGREIPSFKKDIKSLTDGRFHMTLMS
                     IPLIGVLLFTILPLVYMICLAFTNYDHNHLPPKSLFDWVGFANFGNIFSGRMASTFFP
                     VLSWTLIWAVFATVTNFFFGIILALLINTKGLKFKKMWRTIFVITMAVPQFISLLIMR
                     NLLSDAGPVNALLIKWGLISSAHPLPFLSDPVWAKFSIIFVNMWVGIPVTMLVATGII
                     MNLPAEQIEAAEIDGANKFQVFQSITFPQILLIMTPTLIQQFIGNINNFNVIYLLTQG
                     GPTNSTYYQAGSTDLLVTWLYNLTVTAADYNLASVVGILIFILSAVFSLLAYTRTNSY
                     KEGAAK"
     gene            complement(4034..5281)
                     /locus_tag="VBC94_001485"
     CDS             complement(4034..5281)
                     /locus_tag="VBC94_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO6979745.1"
                     /translation="MEKNTWKKLLVSTAALSVVAGGAIAATHSNSVDAASKTTIKLWV
                     PTDSKASYKAIVKKFEKENKGVTVKMIESNDSKAQENVKKDPSKAADVFSLPHDQLGQ
                     LVESGVIQEIPEQYSKEIAKNDTKQSLTGAQYKGKTYAFPFGIESQVLYYNKTKLTAD
                     DVKSYETITSKGKFGQQLKAANSYVTGPLFLSVGDTLFGKSGEDAKGTNWGNEAGVSV
                     LKWIADQKKNDGFVNLTAENTMSKFGDGSVHAFESGPWDYDAAKKAVGEDKIGVAVYP
                     TMKIGDKEVQQKAFLGVKLYAVNQAPAGSNTKRISASYKLAAYLTNAESQKIQFEKRH
                     IVPANSSIQSSDSVQKDELAKAVIEMGSSDKYTTVMPKLSQMSTFWTESAAILSDTYS
                     GKIKSSDYLKRLKQFDKDIAKTK"
     gene            5537..6565
                     /locus_tag="VBC94_001486"
     CDS             5537..6565
                     /locus_tag="VBC94_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6979746.1"
                     /translation="MVTIKDVAAKAGVNPSTVSRVLKDNESISSKTKERVKKAMEELG
                     YVPNVAAQMLASGLTQNIGVIFPPLVLPDRLSEPFFMEILSTLTNEAKEHGFTVSIAT
                     SMSSEELLEQVKLMHLQKRVDGFIVLYSDQKDPIREYLLKYKLPFVVVGAANGYENEI
                     TYIDNDNQLMGKSAIDYLYKKGHKSIQFVTDDLNSEVSEERYLGYFKGARKLGLNQKP
                     ALLFDRGNPQVLEEFINRVKEEETTALIVIGDTVSVRVMQFLSFYKLKVPDDISIMTF
                     NNSLFSHLIHPYLSTFDINVNNLGRTSVRRLIDIIKSPDKVFSETIIVPFTLEERESV
                     RDISKNNN"
     gene            6686..8182
                     /gene="malQ"
                     /locus_tag="VBC94_001487"
     CDS             6686..8182
                     /gene="malQ"
                     /locus_tag="VBC94_001487"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEO6979747.1"
                     /translation="MKKRASGVLMHITSLPGDLGIGTFGREAYAFVDFLVETDQKFWQ
                     ILPLTTTSFGDSPYQSFSAVAGNTHLIDFDLLTLEGFISKDDYQNISFGQDPEVVDYA
                     GLFEKRRPVLEKAVKNFLQEERATRMLSDFLQEEKWVTDFAEFMAIKEHFGNKALQEW
                     DDKAIIRREEEALAGYRQKLSEVIKYHEVTQYFFYKQWFELKEYANDKGIQIIGDMPI
                     YVSADSVEVWTMPELFKLDCDKQPLAIAGVPADDFSDDGQLWGNPIYNWDYHKESDFD
                     WWIYRIQSGVKMYDYLRIDHFKGFSDYWEIRGDYQTANDGSWQPAPGPELFATIKEKL
                     GDLPIIAENLGYIDERAERLLAGTGFPGMKIMEFGFYDTTGNSIDIPHNYTENTIAYA
                     GTHDNEVINGWFENLTVEQKAYAENYMRRLPNEPITETVLRTLYATVSQTTITCMQDL
                     LDKPADSRMNMPNTVGGNWQWRMRKEDLTENRKAFLKEITTIYNRGNK"
     gene            8194..10458
                     /gene="glgP"
                     /locus_tag="VBC94_001488"
     CDS             8194..10458
                     /gene="glgP"
                     /locus_tag="VBC94_001488"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEO6979748.1"
                     /translation="MTRNFTTYVGQQGKVLSELTNEEIYVELLNFVKEEAAAKSKNSS
                     QRKVYYISAEFLIGKLLSNNLINLGIYKDVKKELELVGKSIAEIEDVEPEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFRNNQQEAEANYWIENNSWLVPTDI
                     SYDVPFRDFTLKSRLDRIDVLGYKKDTKNYLNLFDIDGLDYNLIEKGITFDKTEIKKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLLIDEAIERGSNLHDLAEYAYVQINDT
                     HPSMVIPELIRLLTEKHGFEFDEAVSVVRNMVGYTNHTILAEALEKWPLEYLNEVVPH
                     LVTIIKKLDQMIREEQTNPEVQIIDEAGRVHMAHMDIHFSTSVNGVAALHTEILKNSE
                     LKVFYDIYPDKFNNKTNGITFRRWLEFANQDLADYLKELIGDSYLTDATQLEKLLTYA
                     DSNEVHDKLAAVKFKNKLALKRYLKENKGIELDEYSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKRGHFPSRKLTVIFGGKAAPAYTIAQDIIHLILCLSELINNDPEVNKYLNV
                     HLVENYNVTVAEKLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGKENIYTFGKDSDTIINLYETSGYRSKDYYDKDKVIREAVDFIISDDIVSLGNAE
                     RLKRLHDELVGKDWFMTLIDLKEYIAVKEQVLADYEDYESWNKKVIHNIAKAGFFSSD
                     RTIEQYNQDIWHSN"
BASE COUNT         3595 a         1693 c         1974 g         3274 t
ORIGIN      
        1 tgtaacctag ttctttttag atgtctttat ttaactccag acattagttt ttcaatgcgt
       61 ggaacataaa ataagaggat aaatccaaat acaactgtta taccaccaac aacaccataa
      121 taaagaattt gagtatcagg tgtgtacaat ttaacgattt gagcgttaat agcttgtgct
      181 gcagcattac ttaagaacca gatgctcatc atttgagctt ggaatgcttt aggtgcgagc
      241 ttactagttg ctgataatcc aactggtgag attagcattt ccccaacgat gacaatagcc
      301 caactcattg ttaaccaaag agggcttact ttagcgttaa ctccaaagag taaacctggt
      361 agcatcatcc ataagaatga agctccagca aagaaaagtc catacgcaaa ttttttaggt
      421 gatgaaggtt gcttacttcc ccatttagcc cataaccaag caaagaatgg tacataaagc
      481 ataatgaaga gagggttcat actttggaaa taacttgatg ggaaattaat atgatgccca
      541 aagaagttaa ggtaaagttt tgtttgttca tctgcaaata aggctaagac aactgaacct
      601 tgttcttcaa ttgaccaaaa tagaattgag gcgataaaga gagggatata tgcccacact
      661 cgagaacgct caacagaaga aatcttttta ctacttaaaa tcttgataaa ataaataatt
      721 gggatggcga ttgcaataat actaaaaata tcgataatta cttcaattgt tagcatatgc
      781 atcaagtgaa gtagaataag tacaataaca ataccaataa ggataagccc aactttttta
      841 tatagagagg acttttcttc aggacttaaa ggatcgtttg gtctgagact ctcttcagtc
      901 aaatattttt ttccatctaa ggtatattgg aggagaccaa agaacatacc aatagcagca
      961 agtgagaaac caagatggaa atttacttct tgtccgaggt accccactac aatgggtgaa
     1021 ataaaagcac caaggttaat accaaaaaca aagatactaa aaccagcatc acgacgagaa
     1081 tcattttctc catataaatt accaaccatc tctgatacat ttggttttaa tagtccagta
     1141 ccaaggataa ttagagcaat cgaaatgaat agagctgttt gaccaaatgg tgtagctaga
     1201 gcaatatgac ctagcatgat tagaatacca ccatacagaa cagttttacg actacctaga
     1261 atacggtcgc tgacaaaacc accaattact gatgagagat aaaccagtga accatagaca
     1321 gccatgattg atgcagcgac agtcttgtcc ataccaaggc caccttgaga aacactatag
     1381 tacatatagt acagcaaaat agcacgcata ccatagtatg agaatctttc ccacatttca
     1441 gtaaagaaaa gagtggacaa accacgagga tgtccaaaga atgattttgt tttttccatg
     1501 aaaaacgctc cttttgtaga taacactatt aataatatag taaatagaag caactgtcaa
     1561 attacttagc actatcaagt tatttttttg tagaggttaa cacccttggt aaaccaagtt
     1621 ttttaactat ctagcttaga aaaaggcttg aattcaatag aacccaagcc ttttttagaa
     1681 aagaaaccac agtgaaataa aaatgtcgtg tctttaaatt atctgaagat tattttacag
     1741 atccacctgt aataccttca acatagtatt tttgcatgaa catgaaaagt attgtgattg
     1801 gaattgcaat aataacagat cctgctgtaa atgacatgaa ccactggttg atggtatctt
     1861 gttgcaacat tgaaaagaga ccaatagcga ctgtgtactt gctcgtagca tcacctaaaa
     1921 taacttttgc aaagataaag tccatccaag ggcccataaa agcgattaat gcagtataaa
     1981 cgataatggg ttttgacaat ggtagggtga ttttaaggaa aatatccaaa cgggtcgccc
     2041 catcaatcat agcagactca tccaaagagt atggaatagt atcaaagaaa ccttttgcga
     2101 tgtagaaggt taatgctgct cctgcagaat agacaaatat taaagcggtt aatgtttggt
     2161 ctagatttag agcttttagg atgtagtaaa ctgcaatcat tgacatgaac ccaggaaaca
     2221 tattcaagac taaagctagc tttaggaaac cattacggtg tttgaatttg attctgctga
     2281 gagaataagc cattgctaca gtgattaaag ttgatagtat acaagtacat gtagcgacaa
     2341 ataatgtatt taagaaccat tgaccaaatg ggaatgtgtt ttgggtgaag agtttagcgt
     2401 agttgtctaa tgtccaagtt ttaggaataa agtagttgac aaaggcactt ccttcaccac
     2461 ggaagcttgt gagaacgacc caaacaatgg gaaagagcca cataattgat agtactatca
     2521 ataaaatata aacaaaagta aggttaagac gttttttctt attcattatt tagctgcccc
     2581 ttccttgtaa gaatttgttc ttgtgtaagc tagtaaacta aatactgctg aaagaataaa
     2641 gattaagata ccaacaacag atgctaagtt ataatctgca gcagttactg tcaaattgta
     2701 tagccaagtg acaagtaaat cggtgctacc tgcttggtag tatgttgagt ttgttggtcc
     2761 accttgagtg agtaggtaga taacgttgaa gttgttaata tttccgataa attgttgaat
     2821 taaagtagga gtcataatca acaaaatttg tgggaaagta attgattgaa agacttggaa
     2881 cttattcgcc ccatcaattt cagcagcttc aatttgttct gcaggtaaat tcatgatgat
     2941 accagtagca acaagcattg ttacaggaat accaacccac atattaacaa agatgattga
     3001 gaatttcgcc cagaccggat cagaaaggaa tggaagggga tgtgctgaag aaataagtcc
     3061 ccattttatt aacaaggcgt taaccggtcc agcgtcactt aagaggtttc tcatgattaa
     3121 gagagagata aattgaggga cagccatggt aataacaaaa atagtacgcc acatcttttt
     3181 gaatttcaaa ccttttgtat taattagtaa ggcaagaata ataccaaaga agaaattcgt
     3241 tacagtagcg aaaacagccc aaattaatgt ccaagataga acagggaaga aagtactggc
     3301 catacgcccg ctaaagatat taccgaagtt agcaaaacca acccagtcaa ataaactttt
     3361 tggcggtaag tggttatgat catagttagt aaatgccaaa caaatcatat aaactaacgg
     3421 aaggattgta aatagaagca cccctattaa aggaatagac attaaagtca tatggaagcg
     3481 tccatctgtc aagcttttaa tatctttttt gaaggatgga atttctcttc cttcttcttt
     3541 caaggtcaat aaatgtgctg cgcttttaat gtttgaccag taaatatagg caaatacgca
     3601 acagaagata agcgaagcta atccaaaaat tagcattaac atagagttat caccttgtgt
     3661 cgcaacttgg attttaatac catccacagt tttagttgtc ataccttgtt cttgggtccc
     3721 taaggaaata agacctttta cagctgggat aacttggtaa acaaaggtaa ttaaaaacag
     3781 aatttcactg attaaaaata aaagtccctt gacaatttgt ttatttttga ggttagcaag
     3841 tcccataatt aagaatgata gtttagttgc taaatcacct ttttgaaaga cctcttttag
     3901 ggacatacca tcaagtgaga tcagtttttt attcataatg gtctccttta attgttattt
     3961 tacaaaataa gagatgggaa gcactctcaa ttgaaaggaa gagtactcac ctcatctcat
     4021 tactctagac gtactatttt gttttagcga tgtctttatc gaattgtttt agacgtttaa
     4081 ggtaatcgct agatttgatt ttaccactgt aagtatcgct aagaatagca gcactttctg
     4141 tccagaatgt tgacatttga ctcaacttag gcataaccgt tgtatattta tctgagctac
     4201 ccatttcgat aactgctttt gcaagttcat ctttttggac gctatcagaa gattgaattg
     4261 atgagttagc aggaacgata tgacgttttt cgaattgaat tttttgactt tcagcattag
     4321 ttagatatgc agcgagtttg tagctagctg agattcgttt agtgtttgaa ccagcaggtg
     4381 cttggttaac ggcataaagt ttaacgccca agaatgcttt ttgttgaact tctttgtcac
     4441 cgattttcat tgttgggtaa acagcaacac cgattttatc ttcaccgaca gcttttttag
     4501 cagcgtcgta atcccatggt ccactttcaa aagcatgaac agaaccatcg ccaaatttag
     4561 acattgtatt ttcagctgtc aagttgacaa aaccatcatt tttcttttga tctgcaatcc
     4621 atttaaggac agaaacacct gcttcattac cccagttagt gcctttagca tcttcaccag
     4681 atttaccaaa taaagtgtcg cctacagaaa ggaaaagagg acctgttaca tatgagttag
     4741 ctgctttaag ctgttgaccg aatttccctt tgcttgtaat tgtttcgtat gatttaacgt
     4801 cgtcagcagt taactttgtt ttattataat aaagaacttg agattcaata ccaaatggga
     4861 atgcataagt tttcccttta tattgtgcac cagtaagtga ttgtttagtg tcgtttttag
     4921 caatttcttt tgagtattgc tctggaattt cttggataac accagattct actaattgac
     4981 caagttggtc atgtggaagt gagaatacat cggctgcctt gcttgggtct ttttttacgt
     5041 tttcttgagc tttggagtca ttagactcaa tcatttttac agtaacgcct ttgttttcct
     5101 tctcgaattt tttaacaatt gctttataag acgcttttga atctgttggg acccaaagtt
     5161 tgatagttgt ttttgaagca gcatcaactg agttagagtg agtagcagca attgctcctc
     5221 ctgcaactac tgaaagagca gcagtactaa caagtaattt tttccaagta tttttttcca
     5281 ttttggttct cctcctagag aatattttaa ttacacctat attatgcaac cgtttgcaaa
     5341 aaaagtcaag tcttttttta aaaaaaataa gaaaaataaa aaaataatat caattaatac
     5401 taaatgagaa gattatttta gtgagagtat tacatttgca gagaaggcaa acgtttgttt
     5461 ttttcttaga ttagagtata atagacaaag gactatacca aaaggtataa aatgattaga
     5521 atgaagaggt cgtaggatgg taactataaa agatgttgct gccaaagctg gtgttaatcc
     5581 atcgacagta agtcgtgtac taaaagataa tgaatctatt tcaagtaaga cgaaagagcg
     5641 cgtgaaaaaa gctatggaag agctgggcta tgttcctaat gttgcagcac aaatgttggc
     5701 tagtggttta acgcaaaata ttggagttat ttttcctcca ctggttttac cagaccgctt
     5761 aagtgaaccc tttttcatgg agattctttc tacattaact aatgaagcaa aagagcatgg
     5821 gtttacagtt tctattgcaa caagtatgtc ttctgaggag ttgttagaac aagtaaaatt
     5881 aatgcatcta caaaaacgag tggatggttt tattgttctc tattcggatc aaaaagatcc
     5941 tatcagagag tatttattaa aatataaact gccctttgtt gttgttggtg ctgctaatgg
     6001 ctatgaaaat gagattactt atattgataa tgataatcag ctgatgggga aatctgccat
     6061 tgattatctc tataaaaaag gacataaaag catccagttt gttacagatg atttaaattc
     6121 agaagtatca gaagagcgct atttagggta ttttaaaggt gctagaaagt taggtttaaa
     6181 ccaaaagcca gctcttttat ttgacagagg caatccgcaa gttttagagg aatttataaa
     6241 tagagtcaaa gaagaagaaa caactgcttt aattgttatt ggtgatactg tctctgttag
     6301 agtaatgcaa tttctttctt tctataaatt aaaagttcca gatgacattt ctatcatgac
     6361 ctttaataat tctctcttct cacatctgat tcatccttac ctaagcacat ttgatattaa
     6421 tgttaataat ctaggaagga caagcgttcg tcgtctgatt gacatcatta aatctccaga
     6481 caaagtcttt agtgagacta ttattgtgcc ttttacttta gaagagcgtg aaagtgtccg
     6541 agatatttct aaaaataata attaagctat gatataaaga cttctatcaa ttttcgatag
     6601 aagttttttt aaattatgct tgacttatgc aatcgcttgc gttaagatgg aggagtaaag
     6661 taagtaatcg aaaaggagaa tcaatatgaa aaaacgtgca agtggtgtct taatgcacat
     6721 cacttctcta ccaggtgatt taggtattgg aacatttgga cgagaagcct acgcttttgt
     6781 tgattttttg gtcgaaactg atcaaaaatt ttggcaaatt cttcccttaa caacaacaag
     6841 cttcggggac tctccttacc agtctttttc tgcagtagct ggtaatacac atttaattga
     6901 ttttgatttg ctaacgcttg aaggtttcat ttcgaaagat gactaccaaa atattagttt
     6961 tggacaggat ccagaagttg ttgattatgc tggtctgttt gaaaaacgcc gtccagtttt
     7021 agaaaaagca gttaaaaatt tcttgcaaga agagagagct acgagaatgc tatctgattt
     7081 cttgcaagaa gaaaaatggg taactgattt tgctgaattt atggcgatca aagaacattt
     7141 tggtaataag gcgcttcaag aatgggatga caaggctatt atacgccgcg aagaagaagc
     7201 cttagcagga tatcgtcaaa agcttagtga agtgataaaa tatcatgaag taacgcaata
     7261 tttcttttac aaacaatggt ttgagttaaa agaatatgct aatgataaag ggattcaaat
     7321 tatcggtgat atgccaatct acgtttctgc cgatagtgta gaagtttgga caatgcctga
     7381 actgtttaaa ttagattgcg acaaacaacc acttgctatt gctggtgtac cagcggatga
     7441 tttttctgac gatggtcagc tttggggcaa tcctatttac aattgggatt accacaaaga
     7501 gtcagatttt gactggtgga tttatcgtat tcaatctggt gttaagatgt atgactattt
     7561 gcgtattgat cattttaaag gtttttcaga ttattgggaa attcgaggag attatcaaac
     7621 agctaatgat ggctcatggc aaccagctcc cggtccagaa ttatttgcaa ctatcaaaga
     7681 aaaattaggt gatttaccta ttattgctga gaatttaggc tatattgatg agagagcaga
     7741 gagattactg gctggaacag ggttcccagg tatgaaaatc atggaattcg gtttttatga
     7801 tacaacagga aatagtattg atattcctca taactacaca gaaaatacaa ttgcatatgc
     7861 tggaactcat gataacgaag ttattaatgg ctggtttgaa aatcttacag tcgaacagaa
     7921 agcttatgca gaaaattaca tgcgtcgttt gccaaatgag cccattactg agactgtact
     7981 gcgtaccttg tatgcaaccg ttagtcaaac aacgattaca tgtatgcaag atctactaga
     8041 taaacctgca gatagccgta tgaacatgcc aaataccgtt ggaggtaact ggcaatggcg
     8101 tatgagaaaa gaagacttaa cagaaaatcg caaagcattc ttaaaagaga ttacaactat
     8161 ttataataga gggaataaat aaggaatatt atcatgacaa gaaattttac aacttacgtt
     8221 ggacaacaag gaaaagtatt atcagaatta acaaatgaag agatttacgt tgaattgtta
     8281 aattttgtaa aagaagaagc tgccgctaaa tcaaaaaata gctctcaacg taaggtatac
     8341 tatatttcag ctgagtttct aattggtaaa ttactttcaa ataatttgat taacttaggg
     8401 atttataaag atgtcaaaaa agagttagaa cttgtaggta aatcaattgc tgaaatagaa
     8461 gatgtagagc cagaaccatc acttggtaat ggtggtttag ggcgtttagc atcatgtttt
     8521 attgactcga tttcttcttt aggtattaat ggtgaaggtg ttggtttaaa ctatcactgt
     8581 gggcttttca aacaagtatt tcgcaacaat caacaagaag cagaagctaa ctactggatt
     8641 gagaataatt cttggttagt accaacagat atttcttatg atgttccttt ccgtgacttt
     8701 actttaaaat cacgcctaga tagaattgat gtattaggtt ataagaaaga tactaaaaat
     8761 tatttgaacc tttttgatat tgatggactt gattataacc ttattgaaaa aggtatcact
     8821 tttgataaga cagaaatcaa gaagaattta accttgttct tgtatccaga tgattcagat
     8881 aaaaatggag aattattacg tatctatcag caatatttca tggtctcaaa tgctgctcaa
     8941 ttattaattg atgaagcaat cgagcgtggt tcaaaccttc atgatttagc tgaatatgct
     9001 tatgttcaaa ttaatgacac tcacccatct atggtcatcc ctgagttgat tcgtttattg
     9061 acagaaaaac atggttttga atttgacgaa gctgtgtcag ttgttcgcaa tatggttggc
     9121 tatacaaacc ataccatcct tgcagaagct cttgaaaaat ggcctttaga gtatcttaat
     9181 gaggtagtac cccacctagt aacaatcatt aaaaaattag atcaaatgat tcgtgaagag
     9241 caaacaaatc cagaggttca aattattgat gaagctggac gtgttcacat ggcgcatatg
     9301 gatatccact tctcaacaag cgttaatggt gtagcagcat tacatacaga aatccttaaa
     9361 aatagtgaat taaaggtttt ctatgatatt tacccagata agtttaacaa taagacaaac
     9421 ggtattacat tccgtcgctg gttggagttt gctaaccaag atttagcaga ttacttgaaa
     9481 gaactaattg gggatagcta tcttactgat gcgactcagc tcgaaaaatt gttaacttat
     9541 gctgatagca atgaagttca tgataaatta gctgctgtta aatttaaaaa caaactggct
     9601 cttaaacgtt accttaagga aaataaaggt attgaattgg atgaatactc aattattgat
     9661 acacaaatca aacgattcca cgaatataag cgccaacaaa tgaatgcttt atatgttatt
     9721 cataaatact tagaaattaa acgtgggcat ttcccatcac gtaaattaac agttattttt
     9781 ggtggtaaag ctgctccagc ctatactatt gcacaggata tcattcactt aattctctgc
     9841 ttatcagaat taattaataa tgatccagaa gttaataaat accttaatgt ccatttagta
     9901 gaaaattaca atgtgacagt agcggaaaag ttaatccctg caacagatat ttcggaacaa
     9961 atttcattgg cttccaaaga agcatctggt acaggtaaca tgaaatttat gttgaatggt
    10021 gctcttactc ttggtactat ggatggggca aatgttgaaa tagctgaatt agctggcaaa
    10081 gagaacatct acacatttgg taaagactct gatactatca ttaatcttta tgaaacatct
    10141 ggttatcgtt caaaagatta ctatgataaa gacaaagtaa ttcgtgaagc tgttgatttt
    10201 atcattagtg atgatatagt gtcactcggt aatgcagaac gactaaagag attgcatgat
    10261 gagttagtag gtaaagattg gttcatgaca ttgattgatt tgaaagaata cattgctgtt
    10321 aaagaacaag tgcttgctga ttatgaagat tatgaatcat ggaataagaa agttatacac
    10381 aatattgcta aagcagggtt cttctcatca gatcgtacaa tcgaacaata taatcaagat
    10441 atttggcatt caaattaaaa agtctctaaa agtaaggttt tcaaccttac ttttttcttg
    10501 tctaataaaa ctataagtat ttatttttgt ccgtag
//
LOCUS       DATROQ010000066        10373 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.067, whole genome shotgun sequence.
ACCESSION   DATROQ010000066 DATROQ010000000
VERSION     DATROQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10373
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.067"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            119..1183
                     /locus_tag="VBC94_001489"
     CDS             119..1183
                     /locus_tag="VBC94_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6979749.1"
                     /translation="MKRKQFIKLGIATLLTVISLYTPINLATNHTTENIVTAQEYKTK
                     ENGTLPFKHKRQLVLGELDDKGRATFAHIQLKVKDEPKKKRVKRLKTTPVGWHNFKFY
                     YNDGTQKAWLMSRGRLICHQFSGLNNERKNLVPMTNWLNTGNYNSTNSSNPESMLFYE
                     KQLKTWLSTHKNYYLDYKVTPIYQNNELIPRKIELKYVGIDKTGKLLPIFIGNKSTQD
                     QFGISTVTLENTSPNATIDYLSGKAQNTVLSAKEQRKLIAKHEEEKRLAEKKVEEEKA
                     AAETQKKLEEEQARLAAEAQRKQKEEQARLAAETQKKQETLVQEQTSQGYKRDYRGRW
                     HRPNGQYASKAEIAAAGLQW"
     gene            complement(1291..2598)
                     /gene="eno"
                     /locus_tag="VBC94_001490"
     CDS             complement(1291..2598)
                     /gene="eno"
                     /locus_tag="VBC94_001490"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEO6979750.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNVIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEALRWGAEVFHALKKILKERGLETAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDAERKVYDYSKFEGEGGAVRTAAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGGRVQLVGDDFFVTNTDYLARGIK
                     EEAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            2796..3251
                     /locus_tag="VBC94_001491"
     CDS             2796..3251
                     /locus_tag="VBC94_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEO6979751.1"
                     /translation="MTELDNKILQKASGENRLDPDQQRYYLGTYKERVVLHLSLKEAQ
                     EEKFQLAITSALNNLILEYPNLSLKLSPNLPTDVQMFYMKLANRLSLAATIVNEANAN
                     SPFGIIIHSDKAENVEWNDFETQFPDLFNSPKKEESPKKSLWQHFFSQK"
     gene            3264..3749
                     /locus_tag="VBC94_001492"
     CDS             3264..3749
                     /locus_tag="VBC94_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO6979752.1"
                     /translation="MEIWDAYDQYGFITGLTLNRDQNIPQGLFHLVVDVILFHEDGDV
                     LMMKRHPKKKAFPAYFEATAGGSALKGENAKQAILRELKEETGIVPQCLTFLNREWFS
                     ERSYFVDHFIAKYNGAKDIITLQEGETVDYIWLKPEYIDLFLSKNKLIPSQIKLLKSL
                     I"
     gene            complement(3770..4411)
                     /gene="serB"
                     /locus_tag="VBC94_001493"
     CDS             complement(3770..4411)
                     /gene="serB"
                     /locus_tag="VBC94_001493"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEO6979753.1"
                     /translation="MIKGLLVMDVDSTLIMEEAIDLLAIEAGVGKQVAALTDAAMRGE
                     LDFEEALKKRVALLKGLPVTILTDILSSIHFTPGAYELIKECHKRQMKVGLVSGGFHE
                     TIDILAKQLQVDYVKANRLGVKGGFLTGEVEGEIVTKEVKKIKLKEWASENHLDLSQT
                     IAMGDGANDLPMIKSAGVGIAFCAKPIVREEAAYQINEKNLLKVFDIIDANEN"
     gene            complement(4505..6229)
                     /gene="ezrA"
                     /locus_tag="VBC94_001494"
     CDS             complement(4505..6229)
                     /gene="ezrA"
                     /locus_tag="VBC94_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEO6979754.1"
                     /translation="MSSGIILLLVAIVLLVIIAYVVGVVIRKRNDTLIANLETRKQEL
                     VDLPVQEEIEQVKLLHLIGQSQSTFREWNQKWTDLSTNSFKDIDFHLVEAENLNDSFN
                     FVRAKHEIDNVDSQLTIIEEDIVSIREALEVLKEQEEKNSARVTHALDLYETLQKSIS
                     EKEDNYGTTMPEIEKQLKNIEAEFSHFVTLNSTGDPIEASEVLNKAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKDIEQRFQEVREAIRSNSDGLV
                     SLDLDRARDENEHIQEKIDKLYDIFEREIAAYKVAHKDSKIIPQFLAHAKSNNEQLGH
                     EIKRLSAKYILNENESLSLRSFTNDLEEIETKVLPSVENFGQEASPYTHLQILFERTL
                     KTLTTVEENQMEVFEAVKTIESVETRARQNMDKYVNKLHMIKRFMEKRNLPGIPQDFL
                     STFFTTSSQIEALINELSRGRIDIEAVSRLNDVTTNAIANLEQATYLVVQDATLTEQL
                     LQYSNRYRSFEQNVQKSFEQALYLFEVEHNYKASFDEISYALETVEPGVTDRFVTSYE
                     KTQERIRF"
     gene            complement(6323..8275)
                     /gene="gyrB"
                     /locus_tag="VBC94_001495"
     CDS             complement(6323..8275)
                     /gene="gyrB"
                     /locus_tag="VBC94_001495"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEO6979755.1"
                     /translation="MTEETKNMEQRAQEYDASQIQVLEGLEAVRMRPGMYIGSTSKEG
                     LHHLVWEIVDNSIDEALAGFAGHIKVYIEPDNSITVVDDGRGIPVDIQEKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKNGKVHYQEYQRGVVV
                     NDLEIIGDTDLSGTTVHFTPDPEIFTETTVFDFDKLAKRIQELAFLNRGLRISISDKR
                     EGQEVEKEYHYEGGIGSYVEFINENKEVIFENPIYTDGELDGISVEVAMQYTTGYQET
                     VMSFANNIHTHEGGTHEQGFRTALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFNRFLLENPQVAKKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNNAEMNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKVRYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKAYIQPGVNQEEE
                     LRQALDTYSSGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVSVDDAAEADKIF
                     DMLMGDRVEPRREFIEANAVYSNLDI"
     gene            complement(8276..8836)
                     /locus_tag="VBC94_001496"
     CDS             complement(8276..8836)
                     /locus_tag="VBC94_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEO6979756.1"
                     /translation="MNYHDYIWDLGGTLLDNYESSTRAFVETLKEFGYQADHDSVYQK
                     LKESTADAIAYFIPEEADFLKEYKANEAKVLETPILFQGAKELLAKIQRQGSRNFLVS
                     HRDNQVIVILEKTEIIDYFTEVVTADNGFSRKPSPESMLYLKEKYQIDNCLVIGDRDI
                     DKQAGESAGFDTLLVDGSKSLMEIIE"
     gene            complement(8955..10160)
                     /locus_tag="VBC94_001497"
     CDS             complement(8955..10160)
                     /locus_tag="VBC94_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEO6979757.1"
                     /translation="MSQKSNYFKGQIDYAVVIPVFFLLMIGLASIYVATMNDYPSNIY
                     IAMFQQVSWIIMGCIIAFVVMLFSTEFLWKATPYLYALGLTLMVLPLIFYSPQLFAAT
                     GAKNWVTIGSVTLFQPSEFMKISYILMLSRITVSFHQKNRKTFQDDWKLLGLFGLVTL
                     PVMILLMLQKDLGTALVFLAILSGLILLSGISWWIILPILSTIVLFIASFLMIFISPN
                     GKEWFYNLGMDTYQINRLSAWIDPFSFADSIAYQQTQGMVSIGSGGVTGKGFNILELS
                     VPVRESDMIFTVIAENFGFIGSAIVLGLYLIIIYRMLRITIESNNQFYTFISTGFIMM
                     IVFHVFENIGAAVGILPLTGIPLPFISQGGSSLLSNLIGIGLVLSMSYQNTVRQAKAN
                     GSHLKYRSI"
BASE COUNT         3395 a         2054 c         1634 g         3290 t
ORIGIN      
        1 taacttcaca tgtcatacat cattatgtta taatattgtt acaaaaatca cattttaatt
       61 ttcagaatgc atttaaaaat aaacagctac tattttatgt tattttagag gttaacagat
      121 gaaaagaaaa cagtttataa aattaggaat tgcaacctta ctaacggtta tttcgcttta
      181 cacaccaata aacctagcta caaatcatac cacagaaaat attgttactg ctcaagagta
      241 taaaacaaaa gagaatggta ctttaccttt taaacacaaa cgacaattgg ttttaggaga
      301 gttggatgac aaaggtcgtg ccacattcgc acatattcag ctaaaagtta aagatgaacc
      361 caagaaaaag agagtaaaac gtttaaaaac aacccctgtt ggatggcaca attttaaatt
      421 ctattataat gatggtactc aaaaagcttg gctaatgagc cgaggccgtc ttatttgcca
      481 ccaatttagt ggactaaaca atgaaagaaa gaatttagtt ccaatgacga attggctaaa
      541 tactggaaac tacaatagca cgaattcatc taaccctgag agcatgctat tctatgaaaa
      601 acaactaaag acttggcttt cgactcataa aaactactac ttagattata aagtaacacc
      661 aatttatcaa aataatgaac taattccaag aaaaattgaa ttaaaatatg ttggtattga
      721 taagactggg aaattattac ctatttttat cggaaacaaa tccactcaag atcaattcgg
      781 catcagcact gttaccttag agaatacctc accaaatgct accattgact acctcagcgg
      841 aaaggctcaa aatactgtct tatcagctaa agaacaaaga aaacttatcg ctaagcacga
      901 agaagaaaaa cgacttgcag aaaagaaagt tgaagaggaa aaagctgctg ctgaaactca
      961 gaaaaaactg gaagaagaac aagcaaggct tgctgctgaa gctcaaagga agcaaaaaga
     1021 ggagcaagca aggctcgcag ctgaaactca gaaaaaacaa gaaactctag tacaagaaca
     1081 aactagtcaa ggctacaaac gtgactatag aggaaggtgg catcgtccaa atggacaata
     1141 cgcttcaaaa gcagaaatcg ctgctgctgg attgcaatgg tagaactgtt tattatcaaa
     1201 tcaattctat tattataatg tgtacataaa aaccatcttg catatgcaag atggttttta
     1261 ttatattttc aactgtttaa taatctgaga ctattttttt aagttgtaga atgatttgat
     1321 acctttgtat tgagcaactt caccaagttg gtcttcgata cgaagtaatt ggttgtattt
     1381 agcaatacgg tcagtacgtg ataatgaacc tgttttaatt tgaccagcgt tagttgcaac
     1441 tgcgatatca gcgattgttg aatcttcagt ttcacctgaa cggtgtgata caactgcagt
     1501 gtaaccagct tctttagcca tttcaatagc ttcgaaagtt tctgtcaaag taccgatttg
     1561 gttaactttg ataaggattg agttagcagc ttcttctttg ataccacgtg ctaagtaatc
     1621 agtatttgta acgaagaaat cgtcaccaac taattgtaca cgtccaccaa ggcgttctgt
     1681 aagagctttc caaccatccc agtcgttttc atccatacca tcttcaatag tgatgattgg
     1741 gtatttgtta accaactctt caaggtaatc gatttgttct gcagcagtac gaactgctcc
     1801 accttcacct tcgaatttac tgtagtcgta tactttacgt tctgcatcgt agaactctga
     1861 tgaagcacag tcaaaaccaa tcatgattcc gttttcgcca gcttcgtaac cagctgcttc
     1921 aatagcttta agaatagttt ctacgccatc ttcagtacct tcaaatttag gtgcaaatcc
     1981 accttcatca ccaacagctg tttcaagacc acgttcttta aggattttct taagagcatg
     2041 gaatacttca gcaccccaac gaagtgcttc tttgaaagtt ggtgcaccaa ctggcataat
     2101 catgaattct tggaatgcga ttggagcgtc tgagtgagaa ccaccgttga tgatgttcat
     2161 cattggagtt ggaagaactt tagtgttgaa tccaccaagg tagctgtaaa gtggtacttc
     2221 aaggtaatca gctgcagcac gtgctacggc aatagaaaca ccaagaatag catttgcacc
     2281 taatttacct ttattaggag ttccgtcaag agcgatcata gcacggtcga tagcttgttg
     2341 gtcgcgtaca tcataaccaa tgattgcttc tgcaataacg ttatttacat tgtcaacagc
     2401 tttttgtgtt ccaagaccac cgtaacgaga cttgtcgccg tcgcgaagtt caactgcttc
     2461 gtgttcacca gtagaagctc ctgaaggaac cataccacgt ccgaaagcac ctgattctgt
     2521 ataaacttct acttcaagtg ttgggttacc gcgtgagtca aggacttcgc gtgcgtaaac
     2581 atcagtaata attgacatta caatactctc cttatgagtt ttaatttttt acatattcat
     2641 aatatcataa tctttagtta ataacaagga aaaacccagt gaaaccgctt tattacgtgg
     2701 tttttgataa aaaaatcact aattcttttc aagtttacct gtgctaatgc aacccattta
     2761 ggttataata aaattagcaa attcaaggag aaattatgac tgaacttgac aataaaatcc
     2821 tacaaaaagc aagcggtgaa aatcgtcttg atcctgatca acaacgttac taccttggta
     2881 cttataaaga aagagttgtg ctccacttaa gtttaaaaga ggcacaagaa gaaaagtttc
     2941 aacttgctat aacatctgct cttaataatc tgatacttga atatcctaat cttagtttaa
     3001 aattatcacc aaatctccct actgatgttc aaatgttcta catgaagtta gctaaccgtc
     3061 tatcactagc tgctacgatt gttaatgaag caaatgccaa ttctccattt ggtattatta
     3121 tacatagtga taaggctgaa aacgttgaat ggaacgattt tgagacacaa ttccctgatt
     3181 tattcaattc accaaaaaaa gaggagtctc ctaaaaaaag tctatggcaa catttcttta
     3241 gtcaaaaata acaaggaggt ctaatggaaa tttgggatgc ttatgaccaa tatgggttca
     3301 ttacaggtct tacacttaat agggatcaaa atatcccaca aggcttattc cacttagttg
     3361 ttgatgttat cctttttcat gaggatggtg acgtattgat gatgaaacgg caccctaaaa
     3421 agaaagcttt tcctgcttat ttcgaagcca ccgctggcgg atctgccctt aaaggagaaa
     3481 atgctaagca agctattcta agggaactca aagaagaaac tggtatcgtt cctcaatgtc
     3541 taacctttct taatagagag tggttctctg agcgttctta ctttgtagac cattttatcg
     3601 caaaatacaa tggtgcaaaa gatataatta cattgcaaga aggagagacc gttgactata
     3661 tctggttaaa accagaatat attgatttat ttttatctaa aaacaaatta ataccctctc
     3721 aaataaaact cttaaaatcc ttaatctaaa aactaggctt aataaaggcc tagttttcat
     3781 ttgcatcaat aatatcaaat accttaagca aatttttttc attaatctga tacgcagctt
     3841 cttctctgac aattggtttt gcacaaaaag caattcctac cccagcggat ttgatcattg
     3901 gtaaatcatt ggcaccatct cccattgcga tggtttgtga aaggtctaag tgattttcag
     3961 aagcccactc ttttaacttt atttttttca cttctttagt gacaatctct ccctcaactt
     4021 cgccagtgag aaaaccacct ttgacaccaa gacgattcgc tttgacataa tcaacttgta
     4081 attgcttagc taagatatca atcgtctcgt gaaaacctcc tgatacaaga ccaactttca
     4141 tctgacgctt gtgacactct ttaatcaatt cataagcccc tggagtaaaa tgtattgagg
     4201 ataatatatc tgtcaaaata gtaacaggca aacctttaag tagcgctact cttttcttca
     4261 aagcttcttc aaaatctaac tcgccacgca ttgcagcgtc agtcagtgca gcaacctgtt
     4321 tccccacccc tgcttcaata gctaataaat caatagcttc ttccattatt aaggttgaat
     4381 caacgtccat gactaacaaa cctttaatca tttaaaacct ccaaaacttt tttactattg
     4441 taccttaaaa ttagcacctt tcatagatag caattaaaaa aagaagagta ctaactcttc
     4501 ttttttagaa acgaatacgt tcttgtgtct tttcataaga cgtcacaaaa cggtcagtta
     4561 cacctggttc cacagtctca agtgcgtatg aaatttcatc gaaagatgct ttatagttat
     4621 gttcaacttc aaataagtac aaagcttgtt caaagctctt ttgcacattt tgttcaaaac
     4681 tacgataacg gtttgaatac tgtagtaact gctctgtgag agtcgcatct tgaacaacta
     4741 gatatgtagc ttgctctaaa ttagcgatag catttgtagt cacatcattt aaacgtgaaa
     4801 cagcctcaat atcaatacga ccgcgactga gttcattaat aagtgcctca atttgagaac
     4861 ttgttgtaaa gaaagtgctt aagaagtctt gcgggattcc tggaagattg cgtttttcca
     4921 tgaaacgttt aatcatatgg agcttgttaa catacttatc catattttgg cgtgctcgtg
     4981 tctcaacgct ttcaatagtt ttaacggctt caaaaacttc catttgattt tcttcaacag
     5041 tagtcaatgt tttcaacgta cgctcaaata aaatttgtag atgagtataa ggtgaagctt
     5101 cttgaccaaa attttctaca gaaggtaaaa cctttgtttc aatttcttcc aaatcatttg
     5161 tgaaggaacg taaacttaag ctttcatttt catttaagat atatttagca gataatcgtt
     5221 tgatttcatg ccccagttgt tcattgttag attttgcatg agcaaggaat tgaggaataa
     5281 ttttactatc cttatgagct actttataag cagcaatctc acgttcaaaa atatcataaa
     5341 gtttatcaat tttttcttga atatgttcat tttcatcgcg agcacgatct agatccaaac
     5401 taactaaacc atctgaattc gaacgaatag cctcacgaac ttcttgaaaa cgttgttcaa
     5461 tatccttttc agggaaatgg tagttttcct ctaaaagacg acgatatcct gtctccaaat
     5521 catctaactg atctggaaaa tcgtcttcta atttagcaac aatagctgga atttgttctg
     5581 taatttgccc taaagcaatc gtatgctcct ctgctttatt caaaacttca gaagcttcaa
     5641 tcgggtcacc agttgagttt aaggtcacaa aatgtgaaaa ttcagcctcg atatttttca
     5701 actgcttttc aatttctggc atggttgtcc cgtaattatc ttctttttca gaaattgatt
     5761 tttgaagtgt ttcgtatagg tccaaagcat gtgttacacg ggcactattt ttctcttctt
     5821 gctcttttaa aacttctaaa gcttcacgaa tcgaaacaat atcttcttct ataatagtta
     5881 gctgactatc aacattatca atctcgtgct tagcacgcac aaaattaaat gaatcattaa
     5941 gattttcagc ttcaactaaa tgaaaatcaa tatctttaaa tgaattagtt gataaatcag
     6001 tccatttttg gttccattct ctaaaagttg attgactttg gccaatcaaa tgaagcaatt
     6061 tgacttgctc aatctcctct tgcacaggaa ggtcaactaa ctcttgtttt ctagtttcta
     6121 agtttgcaat taatgtatca ttacgttttc ttattacgac gccgactacg taggcaataa
     6181 tcacaagcag gactatagcc acaagcagca atatgattcc gctcgacatt caactctcct
     6241 tataatggtc taattttcta aaaatctact ataacgttaa gattatagca tacttgaact
     6301 gtttttcaaa gctatctttg gactagatat ctaagttact ataaacagca ttagcttcaa
     6361 tgaattcacg tcgtggttct actcgatctc ccattagcat atcgaaaatc ttatctgctt
     6421 ctgctgcatc atcaactgag acacgtgcca tcaagcggtt ttcaggatcc attgttgttt
     6481 cccaaagttg atggtcatcc atctcaccta gacctttgta acgttgtaca gtaggttttg
     6541 aacgtcctga actataggta tctagagctt gacgtaattc ctcttcttga ttgacccctg
     6601 gctgaatata agctttaatc tcacttccta ctttgacacc atagattggt ggttgagcga
     6661 tataaacata tcctgcttcc aaaacaggtc gcataaaacg ataaatcaga gttaaaagta
     6721 aagttctgat gtgagctcca tcaacatcgg catcagtcat aatgactaat ttttgatatc
     6781 tgacttttga tacatcaaaa tctgcaccaa atccagttcc catagctgtg aagagacttc
     6841 ggatttcttc gtttgctaaa attttatcca ttgtagcttt ttcaacgttt agaatcttac
     6901 ccctaatcgg taaaattgct tgaaactcac gattacgtcc tgattttgca gaccctcccg
     6961 cagaatctcc ctccactata aaaagttcat tcatctcagc attattagat gagcaatcag
     7021 ccagtttccc aggtaagttt gaaatctcta agcctgactt tttacgtgtt acttcacgcg
     7081 cccgttttgc tgcaatacgg gctttagatg ccaaaattcc tttttcaaca atcttcttag
     7141 ctacttgtgg attttctaat aggaatcgat tgaaagcttc gctaaagaga cgatttgtaa
     7201 ttttaacaac ttcagaattt ccaagttttg ttttagtttg tccttcaaat tgtggatttg
     7261 gatgtttaac agatataact gctgttaaac cttcacgaac atcttctcca gttaaattat
     7321 cttcattttc tttgagaatc ttattctttt tagcgtaatc attaatcaca cgtgtcaagg
     7381 cagtacgaaa tccttgttcg tgcgttcctc cttcatgagt gtggatgtta tttgcaaaac
     7441 tcataacagt ctcttgatat ccagtagtat actgcattgc tacttcaact gaaataccat
     7501 ctaattcacc gtcagtataa ataggatttt caaaaatcac ttctttgttc tcattgataa
     7561 actcaacgta tgaacctata ccaccttcat agtgatattc tttttcaact tcttgacctt
     7621 ctcgcttgtc tgaaatggag attcttagtc cacgatttaa aaaagctaat tcctgaatac
     7681 gtttggctaa tttatcaaaa tcaaatacag tggtttcagt gaaaatttct gggtcaggcg
     7741 taaagtgaac tgttgttcct gataagtcag tatcaccgat aatttcaaga tcattaacaa
     7801 caacaccgcg ttggtattct tgataatgaa ccttgccatt tttataaacc ttgacatcta
     7861 attgtgttga caatgcatta acaacagatg accctacacc atgtaagcca ccagaaacct
     7921 tgtatccgcc accaccaaat ttaccaccgg catggagaac tgtaaaaact gtctctacag
     7981 ctggtcttcc agttttttct tggatatcta ccggaatacc gcgaccatca tcaacaactg
     8041 taatagagtt atctggttca atgtaaactt taatatgtcc tgcaaatcca gctaaggctt
     8101 catcaattga gttatcaaca atttcccata caaggtgatg aagcccttct ttcgaagtcg
     8161 aaccgatata catgcctgga cgcatgcgaa cagcttctaa accttctaat acttgaatct
     8221 gacttgcgtc atattcttga gcacgttgtt ccatattctt tgtttcttct gtcatttact
     8281 ctattatctc catcaatgat tttgacccat cgactaataa ggtatcaaaa ccagccgatt
     8341 ctcctgcttg cttatctata tcacgatcgc ctattactaa acaattatca atttggtatt
     8401 tttctttcaa ataaagcata ctctcgggac taggtttcct tgaaaagccg ttatcagcag
     8461 tcactacttc tgtaaaatag tcaataattt ctgttttttc cagaataact ataacttgat
     8521 tatcacgatg agacaccaaa aaattacgac taccttgtct ttgaattttc gctaaaagtt
     8581 cttttgcccc ttggaataga attggtgttt ccaaaacctt agcttcatta gccttatact
     8641 cttttaaaaa atcagcttct tcagggataa aatatgcaat tgcatctgct gttgactctt
     8701 ttaatttttg atacacacta tcgtggtctg cttgatatcc aaattctttc aaagtctcta
     8761 caaaagcacg tgtggaactt tcgtaattat caagtagtgt accacccaaa tcccaaatat
     8821 aatcatgata attcataccc tttattatat catattatta cgatttttta tagctaccac
     8881 cttacttaat taactcactc ctctaaaata tttcgattat taaaaggctg gattatcttt
     8941 ccaacctttt tttgttatat actacgatac ttcaaatgtg atccattagc cttggcctga
     9001 cgaactgtat tttgataaga catggataag accaatccaa ttccaataag attacttagt
     9061 aatgaacttc caccttgcga aataaacgga agtgggattc ctgttagagg taatatacca
     9121 actgctgcac caatattttc aaaaacatgg aaaacaatca tcatgataaa accagttgat
     9181 ataaaagtat aaaattgatt attagattca atggtgatcc ttaacatacg gtaaatgata
     9241 atgagatata accctaaaac aatcgccgaa ccaatgaagc caaaattctc agcaatgaca
     9301 gtaaaaatca tgtcactttc acgaacagga accgagagct ctaaaatatt aaaaccttta
     9361 cctgttaccc cgccacttcc aattgatacc ataccctgtg tttgttgata agcaattgag
     9421 tctgcaaaac taaatggatc aatccaagca gataaacggt tgatttgata tgtatccatc
     9481 cccaaattat aaaaccattc cttgccgttt ggtgagataa aaatcatcaa aaagctagct
     9541 ataaaaagaa cgatagtaga taaaattggc aagattatcc accaagatat gcccgataaa
     9601 aggattaaac ctgacaaaat ggcaaggaaa actaatgctg ttcctaaatc tttttgtaac
     9661 attagaagaa tcatcactgg caatgtaacc aaaccaaaca atcctagcaa cttccaatca
     9721 tcttgaaaag ttttgcgatt tttctgatga aaactaacag ttattcgaga taacattaaa
     9781 atataggaaa tcttcataaa ctctgacggt tgaaacaaag ttaccgagcc aatggtcacc
     9841 caatttttag cacctgttgc cgcaaaaagc tgtggactat aaaaaattaa gggaagaacc
     9901 attagggtta gtcctaaagc atacagatag ggcgtggctt tccataaaaa ttcagtactg
     9961 aaaagcataa cgacaaatgc aataatacac cccataataa tccaagacac ctgctgaaac
    10021 atcgcgatat agatattaga aggataatca ttcatcgttg caacataaat tgaggctaaa
    10081 ccaatcatta ataaaaaaaa tactggtatc acaacagcat aatcaatttg ccctttaaaa
    10141 tagtttgatt tttgtgacat aggacttcct tttttattca tggtaattat tataacattt
    10201 tttcagaatc tgacttatat tttttgcaat aaaaaatgca actcaaatca ttatgagttg
    10261 caaatattag gagattgttc ttcatcagta aaacaaagaa catgaaaaga aaagtttatt
    10321 taggatattt aaagtataca aagttcacct taaacaaaac ttaaataatc tta
//
LOCUS       DATROQ010000067        10173 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.068, whole genome shotgun sequence.
ACCESSION   DATROQ010000067 DATROQ010000000
VERSION     DATROQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10173
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.068"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..337)
                     /locus_tag="VBC94_001498"
     CDS             complement(<1..337)
                     /locus_tag="VBC94_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6979758.1"
                     /translation="MSNVKNRLAELIRKSKFTKKELAHEIKVNPRTITRWERSESPIP
                     SDKIKILADLLDTNITYLLGQSDFNLNEITHVGKDYFGDDINILQRDRLEFLKTLKNE
                     IDTLKNTSIS"
     gene            482..679
                     /locus_tag="VBC94_001499"
     CDS             482..679
                     /locus_tag="VBC94_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6979759.1"
                     /translation="MTNNFRVVLAKKRKKIADVAKATGLSKTTLTALYYERTKNPEVE
                     TLLKIANFLGVTLDELLTVEE"
     gene            690..1319
                     /locus_tag="VBC94_001500"
     CDS             690..1319
                     /locus_tag="VBC94_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEO6979760.1"
                     /translation="MNLVYLDGKKEPYTTQDIIAEHAEIDNISVRKLIDKHREDLEAF
                     GVLSFEIHKPHKGSLGGRPRKIYRLNEQQATLLVTYLDNTNPVRVFKQNLVKAFFGMR
                     EELTQIRLQRSLEAPKRKTLNEAIKTWEHAPKMAYPTVYNLLLKAVTGKNSKQLKATR
                     GCYSGIDCLNSIELAQYTALEDMAIALINLNFTYQDIKTMALKNTLQGA"
     gene            1533..2171
                     /locus_tag="VBC94_001501"
     CDS             1533..2171
                     /locus_tag="VBC94_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HEO6979761.1"
                     /translation="MNIIKQVKSSFGELEIDFYLDRNRNIFVTIEQLAQGFGYKSRNA
                     IEKMIERQPYLKEKRFSVTDKLSATDGKQYETRLFNKRGIFEIGMLSKTEKGKIFRQW
                     IYDHIEELERENANFKLIRELEKSNHKELTQAIKDWEHFNQWSYKAISDLLLKSVTGQ
                     TAKQLKQSRVGYEIALDCLSADELTRYRKLEQKVIVLLELNAEYNDIKKLVL"
     gene            2273..2446
                     /locus_tag="VBC94_001502"
     CDS             2273..2446
                     /locus_tag="VBC94_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEO6979762.1"
                     /translation="MSPKMGRPVKGTAKRDKRLEVRLTADEYNAIQETADKNGLSKAD
                     LIVKAVNSYESEK"
     gene            2587..3039
                     /locus_tag="VBC94_001503"
     CDS             2587..3039
                     /locus_tag="VBC94_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979763.1"
                     /translation="MLKNQNNMIKKKRNPRVLFRNVAYKLSEIEGKTLTETASFLGFG
                     NSEVCRSALYKWKRKKWLKFDLKNGHYRNVEVLHEVTLEKMANKELKEQGLIYKANIY
                     YEQVVSTSEIIEDIKTKTQDRIKAIHLQQKALERIPSELFAELYTNMN"
     gene            3128..3460
                     /locus_tag="VBC94_001504"
     CDS             3128..3460
                     /locus_tag="VBC94_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979764.1"
                     /translation="MKYRVETNPFSKDRYTPEQREMFKNRQLSKDKAEVYFTRLYNQH
                     IARVIIANVMAEYTTTFRKSATTFEEAWGALGYKQTTEIVFRAVNGLPCSEKDTGELE
                     TYLSEVSA"
     gene            3460..3651
                     /locus_tag="VBC94_001505"
     CDS             3460..3651
                     /locus_tag="VBC94_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979765.1"
                     /translation="MQELNLTPTQTLILFIVLGLLGLLLSRSKPLIEIDLPEDTQAPK
                     LPQNANYGAYIQSQNHYYN"
     gene            3663..4025
                     /locus_tag="VBC94_001506"
     CDS             3663..4025
                     /locus_tag="VBC94_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979766.1"
                     /translation="MTTEEYFKKLLEDSEKHPTNWLSDRVLDKNIKMLDEDIEAEWDD
                     FPLFTKKIFINTTQRDYNKADAVVKFLESMPIEMEYKKVLNIVIQALNELKDSLKNQV
                     LGVIDNQILFPNDKEDTK"
     gene            4022..4363
                     /locus_tag="VBC94_001507"
     CDS             4022..4363
                     /locus_tag="VBC94_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979767.1"
                     /translation="MTLPENYRRVLNLIKVGADNPITGAEIGLILKLEERSVQSIISN
                     LITRYNVPIVAIRHGFNRGYFIPANKEELLEGAKAFYNQVQKEQERLSVLMNADLESY
                     KKLLKEADMNV"
     gene            4356..4628
                     /locus_tag="VBC94_001508"
     CDS             4356..4628
                     /locus_tag="VBC94_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO6979768.1"
                     /translation="MFSLSKESEQDLTQGVLELVGNYLEVREQTQPRLLGLITAQQIK
                     DELGIKAKTLKRWEDNGLRRYQPPLEDTRKHYYKVSDILIFLGVDT"
     gene            4629..5486
                     /locus_tag="VBC94_001509"
     CDS             4629..5486
                     /locus_tag="VBC94_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEO6979769.1"
                     /translation="MAIYETRGFSSYLYPYKGQLEPFDYIAHFKPLKPPEGIDIEEYK
                     RTQAPYCLSGKVTAEKNGSYKRNNASLVYRDLIFLDYDEIETGVNLPKIVSETLWEYS
                     YIIYPTIKHTPKKPRYRLVVKPSDVMTEATYQQVVKEIADKIGLPFDLASLTWSQLQG
                     LPVTTGDPEDYQRSVNRGLDYPVPKNGSTPNRQVVTTYTPRPRSQRSITMRVIDTLFN
                     GFGDEGGRNVALTKFVGLLFNKWVDCDIETAYELVQIANSVTTNPLPERELDRTFESI
                     ARAEFRKRG"
     gene            5506..7008
                     /locus_tag="VBC94_001510"
     CDS             5506..7008
                     /locus_tag="VBC94_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HEO6979770.1"
                     /translation="MKSLESKILEARENEQPPKTMRELENRIFQAGEQWREEHTETKI
                     NESTGDVTEKVAMPQVFTVAKMLSEIITFTFISKSNVPDYSLLYIYDLDEGIYTASND
                     LFNRFCKTFDVRIKPREWPQIKLMVRTLTRIKKPLESAYFIPVQNGIIDLRTKELLPF
                     SPKYVITSKISTAYHAPKRVPTDREGKTFDDWLNSIACNDSELVTLFWQIILEAINPN
                     HTRNKFAIFYGDGNNGKGTFQRFLINLIGESNVSALKPVQFAEKHNLETLVGKVCNIG
                     DEAPNEYLKNPSDLMSITSGDTVLVNPKGRPAFEATFKLFNVFSGNYIPNGGNKTKGW
                     YRRIMIVPFNADFNGEKEKPWIKNDFLGEKEVLEYALYKAINQESFTHFIEPQAVKGL
                     LEEYQEDNDYLLSWVKHEYMERGWHELDVVPVFIATRSLKHYAEDMGIPKPNVYGAGK
                     ETIRHLQQLTQNKYQLKKARVKVEDLDKLDPLEFERPKLGRVNHAITKKE"
     gene            7342..7515
                     /locus_tag="VBC94_001511"
     CDS             7342..7515
                     /locus_tag="VBC94_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2758 domain-containing protein"
                     /protein_id="HEO6979771.1"
                     /translation="MKIKLFYQKHNESLDDFEYRVNLFTLSVSVIDIKFSEATYGNYE
                     DMSTTTSLLVSYR"
     gene            7521..7694
                     /locus_tag="VBC94_001512"
     CDS             7521..7694
                     /locus_tag="VBC94_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979772.1"
                     /translation="MKLKLHTRGGNTITVQGDRTLYDELVEILLSGRQPNWVKTPSGT
                     INLSEIIAITKEK"
     gene            7696..8253
                     /locus_tag="VBC94_001513"
     CDS             7696..8253
                     /locus_tag="VBC94_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979773.1"
                     /translation="MRTFSDTPKTFTFHYTFRDFDTAQVACHAILGYMTGTYEQPVID
                     ATYHNDDQGGHANQLVLKYVEDRKLSKVFKRICDSFKDYYNQPEDMTDEELDDVAQET
                     ELLKEVESTDKKRVVPLFDNSQEKADKQDIFMAFISDHNQLAEHVSMNYKKMTQEDLG
                     AVLESISQGFNYLYDMAIEGELLVK"
     gene            8326..8814
                     /locus_tag="VBC94_001514"
     CDS             8326..8814
                     /locus_tag="VBC94_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979774.1"
                     /translation="MLELAIESIIKPMKKQEKTRVTGTINDQSITIDLDNLIVHYEGQ
                     EFLLETIPGTYGGKRYFFLCPDCERRCRKLFKASHAFACGTCQKLHQATLNRSKTDCQ
                     YYWQLAFKECLKVDSKAKHKHGYYSYDDFPKRPKYMRMTKYLYHWKRFHYYMDKGDKY
                     WL"
     gene            8821..8973
                     /locus_tag="VBC94_001515"
                     /pseudo
     CDS             8821..8973
                     /locus_tag="VBC94_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889234.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
     gene            8948..9325
                     /locus_tag="VBC94_001516"
     CDS             8948..9325
                     /locus_tag="VBC94_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEO6979775.1"
                     /translation="MSKKNAIRKLKEFHRWQRIANSLDLSYNERYQFDIKYHPTRREH
                     LEISRECALEELDAIKYAINQLSKIDYRKILIECYLISEKKTQQDIIAELNKSQSWYY
                     ETKKRALLEFAQSYRNGVLITTA"
     gene            9394..9681
                     /locus_tag="VBC94_001517"
     CDS             9394..9681
                     /locus_tag="VBC94_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEO6979776.1"
                     /translation="MHALEKNTQVNFKTNSDLLEKAKAIIAAQNLDMTASFNLFLENI
                     VQNKALPFETDTDKERAELLAGLRAEIAKSFDDLEHGRVYNASEVRANLGI"
     gene            9671..10018
                     /locus_tag="VBC94_001518"
     CDS             9671..10018
                     /locus_tag="VBC94_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEO6979777.1"
                     /translation="MVSDNKTHSLIIPETVQEQLQEIKSYIETTYFSEQAGANTVNNI
                     LYGLERLEFFPEAGFNADDRVGETIYPPHNTRCIVLGDYLAFYHILEDRKAVFVSYII
                     HSKQDYIKLFKKK"
BASE COUNT         3603 a         1781 c         2035 g         2754 t
ORIGIN      
        1 cgctgataga agtatttttt aatgtatcaa tctcattttt taaagttttg agaaactcta
       61 atctatctct ttgaagtatg tttatatcat ctccaaaata atctttacct acatgggtaa
      121 tttcattcaa attaaaatct gattggccta ataaataagt tatgtttgta tctaataaat
      181 cagctagaat ttttatttta tctgatggga ttgggctttc cgaccgttcc catctagtaa
      241 ttgtcctagg attcactttt atttcatgag caagttcttt tttagtaaac ttgctttttc
      301 ttataagttc cgctaaacga ttttttacat tagacatatt gggtgctcct tattttacac
      361 cacaaatata tcatcaaagt gacaaatatg tcaacacata ggcaaaaaaa cttgacaagt
      421 gacataattg tcattataat tattttataa agtgacaaat atgtctgtag gaggtcggaa
      481 aatgacaaat aattttagag ttgttttagc caagaagcga aaaaaaatcg ctgacgtggc
      541 aaaagctaca ggtttatcaa aaacaacgct aacagctttg tactatgaac gtaccaagaa
      601 ccccgaagta gaaacgcttt tgaagattgc taactttttg ggtgttacgc tagatgaatt
      661 actaacggtt gaagaatagg aggaacatca tgaatctagt atatttagac ggcaagaaag
      721 agccgtatac gacacaagac atcatagctg aacatgctga aattgataac atttcagtga
      781 gaaagctaat tgataagcac agagaagatt tagaagcctt tggggttttg tcatttgaaa
      841 tccataaacc ccacaaaggt tctttaggtg ggcgacctag aaaaatctat cgcttaaatg
      901 aacagcaggc gactttatta gttacttacc tagataatac caatccagtt agggtgttta
      961 aacaaaacct agtcaaggca ttctttggaa tgcgagaaga actcactcaa attaggttac
     1021 agcgttctct tgaagctcct aaacgaaaaa cgcttaatga agctatcaag acatgggaac
     1081 acgcgcctaa aatggcttat ccgactgtgt acaatctatt gcttaaagct gttactggta
     1141 agaatagcaa gcaattaaaa gcaaccagag gttgttattc tggtattgac tgcttaaact
     1201 caattgagct agcacagtac accgctttag aagatatggc aatcgctctt atcaatctta
     1261 attttaccta tcaagatatt aagacaatgg cattaaaaaa cacgctacaa ggcgcgtgag
     1321 aacaacaaaa aaggctttga cagcgaccaa acttccaagc ctttgactag tataactaaa
     1381 ctcaattaat aaagcaggca agctattatt aaaggggttt tagtaaaaga tttgatactt
     1441 ccattgtatc atactaccgc agagcaggca acgacttaac agttgcagtt ctccccgaca
     1501 aaacaacaat aatcaaataa cgaggtaaaa cagtgaatat cataaaacaa gtaaaaagtt
     1561 cttttggaga acttgaaatt gatttttatc tggacaggaa tagaaatatt tttgtgacga
     1621 ttgaacaatt agcgcaggga tttggatata agagccgaaa tgctattgaa aagatgatag
     1681 agcgccaacc ctacctcaaa gaaaagcgat tttcagttac tgacaaattg tcagctactg
     1741 atggcaaaca atacgagacc cgactattca ataagcgagg aatttttgag atcggtatgc
     1801 tgtccaaaac ggagaaaggt aaaatctttc gtcaatggat ttatgaccat atcgaagaac
     1861 tagaaagaga aaacgctaac tttaaactga tacgagagct tgaaaagtct aatcataaag
     1921 aattaacaca agctatcaag gattgggaac actttaatca atggagctac aaggctatta
     1981 gcgacctttt gctaaaatct gtcacaggac agactgctaa acaactgaaa cagtcacggg
     2041 taggttatga aattgcatta gattgcctaa gtgctgatga gttgacgcga tatagaaaac
     2101 ttgaacaaaa agtaattgtc ctattggagc tcaatgcaga atataacgat attaaaaaat
     2161 tagtccttta acaactgaat agagtacgca aaaaagtttg atttaggggt tgacttatgt
     2221 acgtgcttat catatgatat aagcacgggc ttaaatgaaa ggaggaactc ctatgagtcc
     2281 aaaaatgggc agacctgtaa aaggtacagc taaacgtgat aaacggctgg aagttcgttt
     2341 gaccgctgac gagtataatg caatacaaga aacagcggat aaaaatggat tatctaaagc
     2401 ggatttaatc gttaaagctg taaactctta tgagtctgaa aaataaaaaa gttcctaacg
     2461 tggtatagtt tggcgaccgt acacgttagg aactcccagc acccacaaag taggtacgta
     2521 aatagtatat catgcgtact ctattttgtg aacctaaaat tcatagaaag ggagtgcgct
     2581 ttttgtgtgc tcaaaaatca aaacaacatg attaagaaaa aaagaaatcc gagagtgtta
     2641 tttagaaacg tcgcttataa actatctgaa attgaaggga aaacattaac agaaaccgct
     2701 tcttttcttg gttttgggaa ttcagaagtt tgcagaagcg ccctatacaa atggaagcgt
     2761 aaaaaatggc taaaattcga cctcaaaaac ggtcattatc gtaatgttga agtattacat
     2821 gaagttacgc ttgaaaaaat ggctaataag gaattaaaag agcaaggact catttataaa
     2881 gctaacattt actatgaaca agtggtctcg acatccgaaa ttatagaaga cattaaaacc
     2941 aaaacacaag atagaattaa agccatccac ttacaacaaa aagcgctaga gcgtatccct
     3001 agcgagttat tcgcagaatt atacactaac atgaactaat cagggagcta ccccttaaac
     3061 ctagcatgaa tctagtatag gaaaccggca attacacaga aaataaaatt aagcgagaaa
     3121 aaacataatg aaatatagag tagaaacaaa tcctttttca aaagatagat acactcctga
     3181 acaacgagaa atgttcaaaa atcgccaact cagcaaagat aaagctgaag tctatttcac
     3241 tcgactatat aaccaacata ttgctcgggt gattattgct aatgtaatgg cagagtatac
     3301 gactacattc aggaaaagtg ccactacttt tgaagaagca tggggcgctt taggttataa
     3361 acaaaccaca gagattgtct ttagagccgt taacggttta ccttgttcag agaaagacac
     3421 aggggaatta gaaacttatt taagtgaggt atcggcatga tgcaagaact taaccttaca
     3481 ccaacacaga cacttatttt attcattgtt ttaggtctct tagggcttct tcttagccgt
     3541 tctaagccat taatagagat tgacttacca gaagataccc aagcacctaa actacctcag
     3601 aacgcaaact atggggctta tattcaatca cagaaccatt attacaatta gagaggaact
     3661 gcatgacaac ggaagaatat tttaaaaaat tgctagaaga tagcgaaaaa catcctacaa
     3721 attggttaag tgatagggtt ttagacaaaa atatcaaaat gctagatgaa gatattgaag
     3781 cagagtggga cgattttccg ttattcacga aaaaaatatt tatcaatact acacaacgtg
     3841 actataacaa agctgatgcc gttgttaaat ttttggaatc aatgccaata gagatggaat
     3901 ataaaaaagt gctaaatata gttatacaag cattgaacga gttaaaagat agtcttaaaa
     3961 atcaagtgct tggtgttatt gataaccaaa tcctgtttcc aaatgataag gaggacacga
     4021 aatgacacta ccagagaatt atagacgtgt ccttaatctg atcaaggttg gggcagacaa
     4081 tcccattaca ggggcagaga ttggcttaat actgaaactt gaagaacgtt ccgtccaaag
     4141 tatcatcagt aacttaatca cgcgctataa cgtccctatt gtcgctataa gacatgggtt
     4201 taatcgtggc tacttcatcc cagctaataa ggaagaatta ctagagggtg ccaaagcctt
     4261 ttataaccaa gtacaaaaag aacaagaacg tttgtctgta ttaatgaatg cggacctaga
     4321 gagttataag aagttactga aggaggctga tatgaatgtt tagtttaagt aaagagagtg
     4381 aacaggattt aactcagggg gtactggagc tggtaggaaa ttacctggaa gtacgtgagc
     4441 aaacccagcc cagactatta gggttgataa cagcccagca aattaaagat gaactaggca
     4501 taaaggccaa gacattaaag cgttgggaag ataacggtct aagacgatac caaccaccac
     4561 tagaagatac gaggaagcat tactataaag tcagtgatat tcttattttt ttgggggtgg
     4621 atacgtaaat ggctatttat gaaacaagag gttttagttc ttatctctac ccctacaaag
     4681 gacagttaga accatttgac tatattgctc attttaaacc tttgaaacct cctgaaggca
     4741 tcgatattga agaatacaag cgaacacaag ctccctactg cctgagtggc aaagtcacag
     4801 cagagaaaaa cggtagctat aagcgtaata atgctagttt ggtttaccgt gatttgattt
     4861 ttcttgacta tgatgaaata gaaacaggcg taaacctacc taaaatcgtt tctgagacac
     4921 tttgggagta cagttatatt atttatccaa cgattaaaca cacacccaag aagccccgtt
     4981 atcgtcttgt tgtgaagcct agtgacgtaa tgactgaagc gacttatcaa caagtggtca
     5041 aggagatagc cgataagatt ggactgcctt ttgatttagc tagtcttacc tggtcgcaat
     5101 tacaaggctt acccgttaca acaggcgacc cagaggacta tcagcgctct gtgaaccgtg
     5161 gtcttgatta tcctgttcct aaaaacggta gcacaccaaa cagacaggtt gttactactt
     5221 acacgccacg ccctagaagt cagcgttcta ttaccatgag ggtcatagat accttgttta
     5281 atggttttgg agacgaaggc gggcgcaacg tggccttaac taagtttgtt ggcttgctat
     5341 ttaataagtg ggttgattgt gatatagaaa cagcttatga gttggtacaa atagctaaca
     5401 gcgtgacaac taacccccta ccagagaggg agctagatag gacttttgaa agtatagcaa
     5461 gagcagaatt tagaaagaga ggatagaatc atagaaaagg aagaattgaa aagcctggaa
     5521 agtaaaatct tagaggcgcg tgagaatgag caaccgccca agaccatgag agagctagaa
     5581 aaccgtatct ttcaagctgg tgaacaatgg cgggaagaac acacggaaac caaaataaat
     5641 gaaagtacag gggacgttac cgaaaaggtg gccatgcccc aggttttcac agttgccaaa
     5701 atgctaagcg aaattatcac ctttactttt atcagtaaaa gcaacgtacc tgattatagc
     5761 ctactctata tctatgattt agatgagggc atatatacgg ctagtaatga cctatttaac
     5821 cgattttgta agacttttga cgtgaggatt aagcctaggg aatggcccca gattaagcta
     5881 atggttagga cattgacaag gataaagaaa ccgctggaga gcgcctactt tatccctgta
     5941 cagaatggca ttattgactt aaggactaag gagctacttc ctttcagtcc taaatatgtg
     6001 attacaagta aaatcagtac agcttaccac gcgcctaagc gggtcccaac cgatagggaa
     6061 gggaagacat ttgacgattg gttaaactca atcgcttgca atgatagtga actagtaacc
     6121 ttgttttggc agattatcct ggaggctatc aatccaaacc atacacggaa taagtttgct
     6181 atcttttacg gggacggtaa caacggaaaa gggacgtttc agcggtttct tatcaatctg
     6241 ataggagaaa gtaacgtatc agcattgaag cccgtccagt ttgctgaaaa gcataacctg
     6301 gaaacgctag taggtaaagt ttgcaatatt ggagacgagg cacctaatga atacttaaaa
     6361 aatccgtctg acctaatgag tattaccagc ggggacaccg tgctagtcaa tccaaagggg
     6421 cgcccagctt ttgaagcgac cttcaaactc tttaatgtct tttcaggaaa ctatattccc
     6481 aacggtggaa ataagacaaa gggctggtat aggcgtatta tgattgtccc atttaatgct
     6541 gactttaacg gtgagaaaga aaagccctgg attaaaaatg attttttagg cgaaaaagag
     6601 gtgctagagt atgcccttta taaagctatc aaccaggagt cgtttactca ttttatcgaa
     6661 ccgcaggcag tcaaaggcct gttagaggaa taccaggaag ataatgatta cttgttatct
     6721 tgggtaaaac atgagtacat ggaaagaggt tggcacgagc tggacgtagt ccccgttttt
     6781 atcgctacaa gatccttgaa gcattatgct gaagatatgg gaatacctaa gccaaatgtt
     6841 tacggtgctg gtaaggagac aatcaggcac ttacaacagc taacacaaaa taagtaccaa
     6901 ctcaaaaaag cgcgtgtaaa ggttgaagat cttgacaagt tagacccttt ggagtttgag
     6961 agaccgaaac tggggagagt aaatcacgct ataacaaaaa aagaataatg ttgaccttct
     7021 tttgatgatc taaacccttg cagttactag gattttacaa aatttgttga ccttcttgtt
     7081 accttgttta gaaagaaggt aacgcatcta gacccttgat atacatagct ttatagctgt
     7141 tatgttgacc ttgttacctt ctttctaact ctctatatag gaaaaaagca gtatttatat
     7201 atataagggt aaggagttag aaagaaggtc aacatggtaa caaagaggct aaacccttgt
     7261 ggcagtaagg cggggctatg ttacctagaa ggtaacaaaa tcactaaaga aggtaacaaa
     7321 ttgaaaaaaa ggagctaaaa aatgaaaatc aaactatttt atcaaaaaca caatgaatca
     7381 ctagatgatt ttgaatatcg ggtcaatctc tttactctat cagtatctgt aatagatatc
     7441 aaattttcag aagcaactta tggcaattat gaggatatga gtactacaac ttctttattg
     7501 gtctcgtaca ggtaactagt atgaaactaa aactacacac acgaggtggt aacaccatca
     7561 ccgtacaagg agaccgcacc ctttatgatg aattggttga gattctccta tctggccgac
     7621 aacctaactg ggtaaagact ccttctggca ccatcaattt atcagaaatt atagcaatca
     7681 caaaggagaa ataacatgag aacattttca gatacaccta aaacatttac atttcactat
     7741 acttttagag actttgacac tgcacaagta gcttgtcatg ctatcttagg ttacatgact
     7801 ggtacttatg agcaaccagt gattgacgca acttatcaca atgatgacca aggtggtcat
     7861 gctaatcagt tagtcttaaa atatgttgaa gacagaaagt taagcaaggt cttcaagcgt
     7921 atctgtgaca gctttaagga ctattacaac caacctgagg atatgacgga tgaagaactt
     7981 gatgacgtag ctcaagaaac tgagttacta aaggaggttg aaagtactga caagaagcgc
     8041 gtggttcctt tatttgacaa tagccaagag aaagcggaca aacaagacat attcatggct
     8101 ttcatctcag accacaacca actcgccgaa cacgtttcca tgaattataa aaagatgact
     8161 caagaggatt taggagctgt acttgaatct atcagccaag gctttaatta tttgtatgat
     8221 atggctattg agggtgagtt actggttaaa taaacaatca gagggatttc cctctttttg
     8281 tcgtttatca atagttttgg gttatttgaa tactaaggag aaaagatgtt agaactagct
     8341 attgagagta tcattaaacc aatgaagaaa caggaaaaga ctagagttac aggaacaata
     8401 aatgaccaat ctatcaccat agacctagat aacctaatcg tccattatga ggggcaagaa
     8461 ttcttacttg aaacgatacc aggaacttat ggtggtaagc gctacttctt cctgtgtcct
     8521 gactgtgaga gacgttgtag aaagttattt aaggcttctc atgcctttgc ttgtggtact
     8581 tgtcagaagc ttcatcaagc gacactcaat cgaagtaaga cagattgtca atactattgg
     8641 caactagcct ttaaagagtg tctgaaagta gattcaaaag caaaacacaa acatggttat
     8701 tatagttatg atgactttcc taagcgtcca aagtacatga gaatgactaa atacttatat
     8761 cattggaaga gattccatta ctatatggat aagggggaca aatactggct ataacattcg
     8821 gaaaaacaga tagctgttat caaagctaga tacgtggatg acttatcatg ggatgagata
     8881 cccgacaagc taggttattc aaggaatact gcattcaaat tacatagaga agctttagag
     8941 gtattagatg agcaagaaga acgctattcg taaattaaaa gagtttcata gatggcaacg
     9001 tatcgccaat agccttgatt taagctataa cgagcgttac cagtttgata taaaatatca
     9061 tcccacgcgc agagaacacc ttgaaataag ccgagaatgt gccttagagg agctagacgc
     9121 tatcaagtat gctatcaacc aactgtctaa gatagactac agaaagatat tgattgagtg
     9181 ttacttgatt agtgagaaaa aaactcagca agacatcata gcagaactta acaaaagtca
     9241 aagttggtac tacgagacta agaaaagagc tttgcttgaa ttcgcccaga gttataggaa
     9301 tggtgtgtta ataacaacag cataggctct tggatatatg caactataca ggtatagttt
     9361 aaagtgttat aataactaaa aggaggtgct actatgcacg ctttagaaaa aaatacgcag
     9421 gtcaacttta aaaccaacag tgatttacta gagaaagcta aagctattat cgcagcccaa
     9481 aaccttgaca tgacagctag ctttaatttg tttttagaaa acattgtaca aaataaagcc
     9541 ttaccttttg agactgacac cgataaagaa cgagctgaac tacttgcagg tttacgagct
     9601 gaaattgcta aaagttttga cgatttagag catgggcggg tatacaacgc tagtgaagtg
     9661 agggctaacc ttggtatctg ataataaaac ccatagcctt attatccctg aaaccgtaca
     9721 agaacaatta caggagataa aaagctatat tgaaactact tacttttcgg agcaggcagg
     9781 ggctaacacc gtcaacaata ttctttacgg gctagaacgt cttgaatttt tcccagaagc
     9841 aggatttaac gcagatgaca gagtaggtga aactatttac ccgccacata atactcgttg
     9901 tattgtttta ggggactacc tagcttttta tcacatttta gaagacagaa aagccgtttt
     9961 tgtatcttat attatccata gcaaacaaga ctatatcaaa ctgtttaaga aaaaataaaa
    10021 acgcaagatt tacagcaaac taaaaagcac ctttattggg tgctagttct tgcctgctga
    10081 actcattgat tgacagagtt gtaaattaaa tttttgctta ctttttgcta actttgaagg
    10141 aaattttaat gtcaactaat gttgacaaaa aaa
//
LOCUS       DATROQ010000068        10042 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.069, whole genome shotgun sequence.
ACCESSION   DATROQ010000068 DATROQ010000000
VERSION     DATROQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10042
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.069"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..207)
                     /locus_tag="VBC94_001519"
     CDS             complement(<1..207)
                     /locus_tag="VBC94_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEO6979778.1"
                     /translation="MEKTMDMEQREEVVSYNRAKVWQLVMFAMNNASTNIYLFTFMFV
                     TYFSTGVLGSAAIFVSQIMGYIRIF"
     gene            complement(414..1406)
                     /locus_tag="VBC94_001520"
     CDS             complement(414..1406)
                     /locus_tag="VBC94_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEO6979779.1"
                     /translation="MKLKVFNVREEEATLAQDWANRNHVELSMSEGPLTLETVNEVEG
                     FDGIANAQIEPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLELAKQHGIIISNVPSYSP
                     ESIAEFTVTIALNLIRKVELIRANVREQNFSWTLPIRGRVLGNMTVAIIGTGRIGLAT
                     AKIFKGFGCRVIGYDIYHNPMADGILEYVNSVEEAVEEADLVSLHMPPTAENTHLFNL
                     DMFKQFKKGAILMNMARGALVETKDLLEALDQGLLEGAGIDTYEFEGPYIPKNCQGQD
                     ISDKDFLRLINHPKVIYTPHAAYYTDEAVKNLVEGALNACVEVIETGTTTTKVN"
     gene            complement(1420..2478)
                     /locus_tag="VBC94_001521"
     CDS             complement(1420..2478)
                     /locus_tag="VBC94_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO6979780.1"
                     /translation="MSDVVEKQTTKSFIMNVLNGLALGTVIVLIPGAILGELMKALLP
                     MWSGFATLIAATAVATSMMGLVIGIMVGLNFKFNPIQSASLGLAVMFAGGAATFLKGA
                     IMLKGTGDIINMGITAALGVLLIQFLSDKTKSFTLIVIPTVTLLLVGGVGHVLLPYVK
                     MITTMIGQGIASLLGLQPVLMSILIAMIFCFLIVSPITTVGIALAISLSGIGSGAANL
                     GICAASFGLCMAGWSVNSKGTALAHVLGSPKISMANVLAKPKIMLPMISSAAILGILG
                     ALFNIQGTPASAGFGISGLIGPINALNLAKGGWSVMNMLLIIIIFVAAPIILNFIFNY
                     LFIKVLKIIDPMDYKLDI"
     gene            2671..3555
                     /locus_tag="VBC94_001522"
     CDS             2671..3555
                     /locus_tag="VBC94_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6979781.1"
                     /translation="MDIRQLTYFIAVAEAKNYSRAAKSLFVTQPTLSQSIKKLEAELN
                     TILFLQNGRQLALTEAGEILYEKGQLLMTNVNQMVTEIQQLNQEKKEGIRVGLTSLFA
                     IQFMKQISTFMATHSNVEVSLIQDGSRKLQELLAKGKIDIGLLSFPSTRNDITIEPLQ
                     TSTKGYKVSIVMPKSHPLATLPEIELNDLRDYKVASLNEHYMLGEMLPRKCRSLGFDP
                     HIVFKHNDWEVLIHSLQDLNAVTILPSEFESISQVQDLCWVPLKDKNNFYPIGIAYRN
                     DTSFSPMIEEFLSLLKTN"
     gene            complement(3592..4410)
                     /locus_tag="VBC94_001523"
     CDS             complement(3592..4410)
                     /locus_tag="VBC94_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEO6979782.1"
                     /translation="MILKICRAAYSLQWGGVYQLALLDYPRIKAFELERIGAFIAYEK
                     QYKRKTEIQCDDKHLLAKIVHFLKYNSFTFPYIPKYREAAATFNEDGISLTSDFLSHT
                     CTIETAKLIFKEGKILSAVKAFNKPAEVLVKDKRNAAGDPKDYFDYVMLNWSNTNSGY
                     RLVMERLLGKAPSEQELTVGFKPGVSFHFTYQDIINHPDSIFDGYHPAKIKNQLSLAE
                     HLVACVIPKHYQEDYQSLVPNDLKHRVYYLDYCNETLYEWNQKVYDFLCHLENK"
     gene            complement(4574..5347)
                     /gene="fetB"
                     /locus_tag="VBC94_001524"
     CDS             complement(4574..5347)
                     /gene="fetB"
                     /locus_tag="VBC94_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEO6979783.1"
                     /translation="MSGANSISLMSLLMASSLVLITLFFSYWQKLNLEKEVIISAIRA
                     VIQLLAVGFLLDYIFGYQNPIFTALLMLFMIINASYNAAKRGKGINKGFVISFIAIGS
                     GTIITLSVLIFSGILKFVPNQMIPVGGMIISNSMVAIGLCYKQLLSEFRSKQEEVETK
                     LALGADILPASIDIIRDVIKTGMVPTIDSAKTLGIVSLPGMMTGLILAGTSPIQAVKY
                     QMMVTFMLLATTSIASFVATYLAYKIFFNNRKQLVVTKD"
     gene            complement(5344..6000)
                     /locus_tag="VBC94_001525"
     CDS             complement(5344..6000)
                     /locus_tag="VBC94_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6979784.1"
                     /translation="MSYLTFKHVDFKTDDKLVLNDINFAIDEGDFVSIVGPSGSGKST
                     VLKLASGLMSPTAGHIFFDGKDLNQLEPIESRKMISYCFQTPHLFGNTVEDNISFPYH
                     IRHEKVDYRRVDDLFQRFEMDQSYLKQDVKKLSGGEKQRIALIRQLLFEPKVLLLDEV
                     TSALDNHNKAIVEKVIKSLHDKGITILWITHDEEQSRRFANKVLKVVNGSIEAMEVIK
                     "
     gene            6217..6855
                     /locus_tag="VBC94_001526"
     CDS             6217..6855
                     /locus_tag="VBC94_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEO6979785.1"
                     /translation="MFLIDFILHIDTHIYAMANTVGNWTYLLLFLVIFVETGAVIFPF
                     LPGDSLLFAAGALAANPKMSFNIVTFLIIFFIAAFIGDSCNFLIGRTFGYRFIKHPFF
                     RRFIKEKNIRDAELYFEKKGTAAIILGRYIPIIRTFVPFVAGISQLPPKVFIKRAFIA
                     ALSWSVIATGSGFLFGNIPFVKQHFSLIILGIVFVTLIPVLISGVKSYRNTK"
     gene            complement(6933..7718)
                     /locus_tag="VBC94_001527"
     CDS             complement(6933..7718)
                     /locus_tag="VBC94_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6979786.1"
                     /translation="MKRLHKLFITVIATLGMLGVMTFGLPTQPQNVTPIVHADVNSSV
                     DTSQEFQNNLKNAIGNLPFQYVNGIYELNNNQTNLNADVNVKAYVQNTIDNQQRLSTA
                     NAMLDRTIRQYQNRRDTTLPDANWKPLGWHQVATNDHYGHAVDKGHLIAYALAGNFKG
                     WDASVSNPQNVVTQTAHSNQSNQKINRGQNYYESLVRKAVDQNKRVRYRVTPLYRNDT
                     DLVPFAMHLEAKSQDGTLEFNVAIPNTQASYTMDYATGEITLN"
     gene            7979..9424
                     /locus_tag="VBC94_001528"
     CDS             7979..9424
                     /locus_tag="VBC94_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEO6979787.1"
                     /translation="MKKTIGIIGMGVSGLAVLLALSRKKEKELKQLAIFCFDDTVHFG
                     RGIPFQEDVDSALINSPINDISFDYRHMSDFMDWLEENYYDTNLTYVSRTLYGQYLSE
                     RTKLLLDKLPVTVIKTRVDKLNYLSDEQQWQVYIQDKLFPVTFDELHLACGALPVIEP
                     YHLEGKTDYIKDPYPIKYLAQENWKGKTVAIIGTGLAAIDVIKWLLLNTQSSVKAFSR
                     TNYFPSVRIVNDLPLTWHFFTNDNLKAFLSHPSKTFTIADFNDLMSGELEHLGFKNWK
                     NTTETFLSPGIDGLKLAFKYPKQLYLLQQLASRITVWLTDLWPLMSLSDKKEYKALYG
                     KAIINLRNPMPDESAKVILKAAQKGTFSLINNVKTITKNDEGFSIEKEKQNHISVDRV
                     INATGYQLNTKNWTKANPLLKSLINQEFCQIDDQGGITILTENAQVLSPKYGPMPGLY
                     AHGALVNGVVYQNNSTIKIQQMAERAFSDNK"
     gene            complement(9481..>10042)
                     /locus_tag="VBC94_001529"
     CDS             complement(9481..>10042)
                     /locus_tag="VBC94_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979788.1"
                     /translation="SGSGKTTLLSLMAGLDSPTEGQVLFNKKDIKEAGYAQHRKKNIA
                     LVFQNYNLLDYLTPLENVQLVKPTADKQLLLDLGLKEDMLTRNILRLSGGQQQRVAIA
                     RALVVGTPAILLDEPTGNLDFDIARDITMRLKDFAHKEKRCVIMVTHSREIAHMADTA
                     LQLIGDNLKELSKESIAQSILQQRNL"
BASE COUNT         3305 a         1887 c         1521 g         3329 t
ORIGIN      
        1 aaaaatacgg atataaccca taatttgact gacaaaaatg gctgccgagc caagaacgcc
       61 agttgagaaa taggtaacaa acataaacgt aaataagtat atatttgtag aagcattgtt
      121 catggcaaac ataactaact gccatacttt cgctcggtta taagatacaa cttcttcacg
      181 ttgttccata tccattgttt tctccatgag agaaccctcc ttgtattttg taaacgcttt
      241 tatgattaaa taatactatt catctgatgt ttttttcaat atttttataa aaaatatctt
      301 ttttattata aaaaagactt tccagctata aatacataag atgttttttt gctttaaagg
      361 tcataatcat catatgtttt gttaccccac aaaaaaagat gcctaagcat cttttagttg
      421 actttcgtag tagttgttcc cgtttcaatc acttctacac aagcatttaa ggcaccctct
      481 actagatttt tgaccgcttc atctgtataa taagccgcat gaggagtgta tattactttc
      541 ggatggttaa tcagtcttag gaaatccttg tctgaaatgt cttgaccttg acaattttta
      601 ggtatatatg gcccctcaaa ttcataagta tcaataccag ctccctccaa aagcccttga
      661 tctaaagcct caagaagatc tttggtttcc accaatgctc cacgtgccat attcattaaa
      721 atagcacctt ttttaaactg tttaaacata tctaaattaa atagatgcgt attttcagca
      781 gttggtggca tatgtaagga cacaagatca gcttcttcaa cagcttcttc taccgaattg
      841 acatactcaa ggataccatc tgccattgga ttatggtaaa tatcataacc aataacacga
      901 catccgaacc ctttaaaaat tttagcagtc gctagaccaa tgcgaccagt tcctattata
      961 gccacagtca tgttacctag gactcgtcct cgaattggca aggtccatga aaaattctgt
     1021 tcacgaacat tagctctaat aagctcaact tttcgaataa ggttaagagc gattgtcact
     1081 gtaaattctg caatagactc cggtgagtaa cttggcacat tgctaatgat aataccatgt
     1141 tgcttagcta actcaagatt atacatatca acaccagcac tgcgctgagc gatttgctta
     1201 attcccattt ctttgagaag aggataaata gcatcatcta aaggctcaat ctgtgcatta
     1261 gcaatcccat caaaaccttc tacctcatta acagtttcca atgtcaaagg tccttcactc
     1321 atagaaagtt ccacatgatt tcggtttgcc caatcttgtg ctaatgttgc ttcttcttct
     1381 ctaacattaa atactttgag tttcatcact aatatctcct taaatatcta atttatagtc
     1441 cattggatca ataattttaa gtacttttat aaagaggtaa ttaaaaataa aattcaatat
     1501 aattggtgca gcaacaaata ttataattat caataacata ttcataactg accatccacc
     1561 ttttgctaaa ttaagagcat taatcggtcc aatcaaacca ctgataccaa aacctgcact
     1621 agctggtgtt ccttgaatat taaagagagc tccgagaatt cctagtattg ctgctgaaga
     1681 tatcataggt agcataatct tcggttttgc taatacatta gccatcgaaa tcttaggaga
     1741 ccctaaaaca tgcgctaagg ctgtcccctt agaattgact gaccacccag ccatacaaag
     1801 cccaaaacta gcagcacaga tacctaaatt agcagcacca gaaccaattc cggacaaact
     1861 aattgccaaa gcaatcccaa ctgttgtgat tggagatacg atcaagaaac agaaaatcat
     1921 cgcaattaaa attgacatca aaacaggttg taaaccaagt aatgaagcaa tcccttgtcc
     1981 aatcatggtg gtaatcattt tgacataagg taataacaca tgacctactc ctccaactag
     2041 taagagtgtt actgttggaa tgacaatcaa agtgaatgat ttcgtcttgt cagataaaaa
     2101 ttggatcaaa aggacaccta aggcagcagt aattcccatg ttaatgatat cgcctgttcc
     2161 ttttagcata atagcgcctt taaggaaggt tgcagctcca cctgcaaaca taaccgctaa
     2221 tcctagtgat gctgattgaa tgggattaaa tttaaaattg agaccaacca taataccaat
     2281 aacaagcccc atcatacttg ttgcaactgc tgttgcagcg attaaagttg caaagccgct
     2341 ccacattggc agtaaagctt tcattaattc tccaaggatt gctcctggta tcaagacaat
     2401 aacagtccct aaagctaaac catttaatac gttcattata aacgatttag ttgtttgttt
     2461 ttcgacaaca tccgacataa gatgcctcct actaataatg attgtttgtg atttcactaa
     2521 ctatattaac tatatcatag ctatttgcaa aaggaaaatt ggaaaaaatt atataagtat
     2581 agcttttact aatattattg tggaaataat cataaaggtc agattgatag acttgtgcta
     2641 tactattact aagaaaaaag gacagatatt atggatatta gacaacttac ttactttatt
     2701 gctgttgcag aagcaaaaaa ttattcacgc gctgcaaaaa gtctttttgt gacccaacct
     2761 acactttcac aatccattaa aaaattagaa gcagaattaa atactatttt attcttacaa
     2821 aatggtcgtc aactcgcttt aactgaagca ggagaaattc tctatgagaa aggacaattg
     2881 ttgatgacta atgttaatca aatggttacg gagatccagc aattaaatca agaaaaaaag
     2941 gaaggtatcc gtgtagggct tacttctctt tttgctattc aatttatgaa acagatttca
     3001 acttttatgg ctacacattc aaatgttgaa gtatctttga ttcaagatgg ttcccgtaaa
     3061 ttacaagaat tgttagcaaa aggaaagatc gatattggct tattgtcctt tccaagtacc
     3121 cgcaatgata tcactatcga accactgcaa acctcaacta aaggatataa agtaagcatt
     3181 gtcatgccaa aatcgcatcc tcttgcaaca cttcctgaaa ttgaacttaa tgatttaaga
     3241 gattataaag ttgcctcact caacgaacat tatatgctag gagagatgtt gcctcgtaaa
     3301 tgtcgttctt taggatttga cccccatatc gtctttaaac acaatgactg ggaagttctt
     3361 attcatagtt tacaagacct caatgcagtc actattttac caagtgaatt tgaaagcatt
     3421 agccaagttc aagatttatg ctgggtccca cttaaggata aaaataattt ttatccgatt
     3481 ggtattgctt accgaaatga tacttctttt agtcctatga ttgaagaatt tttaagtctt
     3541 ttaaagacca attaatagca gttaaaccaa aaagttttaa actttttggt tttatttatt
     3601 ttccaaatga caaagaaaat cataaacttt ttgattccac tcataaagtg tttcgttaca
     3661 gtaatctaaa taataaaccc tgtgtttcaa gtcattgggc acaaggcttt gataatcttc
     3721 ttgataatgt tttgggataa cacatgcaac taaatgttct gctaaagaaa gctgattttt
     3781 aattttagca ggatgataac catcaaaaat agaatcagga tgattgatga tatcttgata
     3841 agtaaaatga aaactgaccc ctggcttaaa acctactgtt aactcctgtt cagatggtgc
     3901 tttgcctaac aatctttcca ttactaaacg ataaccagaa ttggtatttg accagttcaa
     3961 catcacatag tcaaagtaat ctttagggtc tccagcagca ttcctcttat cttttaccag
     4021 tacttcagca ggcttattaa aggctttaac tgctgataag attttacctt ctttaaaaat
     4081 tagttttgca gtttcaatcg tacatgtatg gcttaaaaaa tcagaagtta aactaatacc
     4141 atcctcatta aaagtagctg ccgcttctct atatttggga atatagggaa aagtaaaact
     4201 attgtatttt aaaaaatgaa caatttttgc gaggagatgt ttatcgtcac attgtatctc
     4261 agtttttctt ttatattgtt tctcgtaagc tatgaaagct cctatccttt ccaattcaaa
     4321 cgccttaatt cgaggataat ccagcaaagc taattggtaa acacctcccc attgtaaact
     4381 atatgctgca cgacaaattt ttaaaatcat ttcttcctcc ttttgataat ccctacaaca
     4441 atttctatta aatagtagca tttaaacgta atcgactcaa taacttggtc aattttcgat
     4501 caaaaataag attaagatac caaaaaagcc tggactatac ttaccagact aacatactgt
     4561 ttattaaaat gaatcaatct tttgttacta ctaattgctt acgattatta aagaaaattt
     4621 tataagccaa ataagtagct acaaaagaag ctatcgaagt cgttgctaat agcataaacg
     4681 tcaccatcat ttggtattta accgcttgga taggtgaagt tccagcaaga attaaccctg
     4741 tcatcatccc tggcaaagat acaatcccaa gtgttttagc tgaatcaatt gtaggtacca
     4801 taccagtttt gataacatca cgaataatat caatcgaagc tggcaaaata tctgctccta
     4861 aagctaattt tgtctccact tcctcttgtt tacttctaaa ttcggacaat aattgtttat
     4921 agcacaaacc gattgctacc atagaattac taataatcat accacctaca ggaatcattt
     4981 gattcggaac aaattttaaa attcctgaaa aaatcaagac agataaagtg attatagtcc
     5041 ctgacccaat agctataaat gaaataacaa accctttatt aatcccttta ccacgcttag
     5101 ctgcgttata agaagcatta ataatcatga atagcattaa aagagctgtg aaaataggat
     5161 tttgataacc aaatatatag tctagcaaaa aacccactgc taataattga atcactgctc
     5221 ggatagcgct aataatcact tctttttcta ggtttaactt ttgccagtaa gaaaaaaata
     5281 aagtaatcaa aactaatgac gatgccatta gtaatgacat taaagaaata gaatttgcac
     5341 cgctcatttt ataacctcca ttgcttcaat actaccattg acaactttta agactttatt
     5401 ggcaaatcga cgactctgtt cctcatcatg cgtaatccac aaaatcgtta ttcctttgtc
     5461 atgtaatgat ttaataacct tttcaacaat agctttatta tgattgtcta aagccgaagt
     5521 aacttcatct aagagtaaaa ctttaggttc aaacaataat tgcctaatca gagcaatacg
     5581 ttgcttttct cctcctgata gttttttgac atcctgtttc aggtaagact ggtccatttc
     5641 aaaacgttga aataaatcat caacgcgtct ataatctact ttttcgtgtc gaatgtggta
     5701 aggaaaacta atattgtctt ccactgtatt accaaataaa tgtggcgttt gaaaacagta
     5761 agaaatcatt tttcgacttt caatcggttc taattggttc aaatcttttc catcaaagaa
     5821 aatatgacct gcagtaggac tcattaaacc actggctaat tttaatacag tactcttacc
     5881 acttccagat ggccctacaa tagaaacaaa atcaccttca tcaattgcaa agttgatatc
     5941 atttaaaacc aacttatcat ccgttttaaa atcaacgtgt ttaaaggtta aataggacat
     6001 tgttaagctc ctttatttct tatttataat cattataatt aagtctaaca taaaaatcaa
     6061 aaaaaagctc tttagaataa ttctaaatta ataagtaaac ttattttacc tcttattaaa
     6121 aattttctga taaaataggc tttgtaagag gccttataaa caagatagtt atcatccaag
     6181 tctctgcact ttagttgatc aataaaggag actcctatgt ttttaatcga ttttatttta
     6241 catattgata ctcatatcta tgctatggct aacactgttg gtaattggac ctatctattg
     6301 ctcttcctcg ttattttcgt tgaaactggg gctgtcatct ttccattttt accaggtgat
     6361 agtttattat ttgctgctgg agcccttgct gcaaatccaa aaatgtcctt caatattgta
     6421 acttttctta ttattttctt tattgcagca tttattggtg atagttgcaa cttcttgatt
     6481 ggtcgtacat ttggttaccg ttttatcaaa catcctttct ttcgtcgttt cattaaagaa
     6541 aaaaatatcc gagatgctga gctttatttt gagaaaaaag gtactgctgc catcatttta
     6601 gggcgctata tccctattat cagaaccttt gttccttttg tagcaggtat tagtcagctc
     6661 cctccaaaag tatttatcaa acgcgccttc attgcagcat tatcttggtc agttattgca
     6721 acaggttctg gttttctctt tggtaatatt ccttttgtga agcagcactt ctcgcttatt
     6781 atcttgggaa ttgtatttgt taccttaatt cctgtcctta tttctggtgt caaatcatat
     6841 cggaacacta agtaatgtat ccaaaaattt ctatttaaca ctattaatca ctaagaatga
     6901 caacaaaaaa agagcaatta gttgctcttt tattaattta gtgttatttc tcctgttgca
     6961 taatccatag tgtatgatgc ttgtgtgttt ggaatagcaa cattaaattc taatgtgcca
     7021 tcttgtgatt tagcttctag gtgcattgca aatggaacta aatcagtatc attacggtac
     7081 aatggagtta cacggtaacg aacacgtttg ttttggtcaa ccgccttacg aactaagctt
     7141 tcataataat tttgtccacg attgattttt tgatttgatt ggttggaatg agctgtttgt
     7201 gtgacaacat tttgaggatt tgacacggaa gcatcccaac ctttgaaatt tccagctaaa
     7261 gcataggcaa ttaaatgccc cttgtcgact gcatgcccat aatggtcatt agtagctact
     7321 tgatgccaac ctaatggttt ccaatttgca tcgggaagag tggtatctct gcgattttga
     7381 tattgacgaa tggttctatc aagcattgca ttagcagttg atagtctttg ttgattgtca
     7441 attgtatttt gaacatacgc tttaacattg acatcagcat ttaaatttgt ctgattatta
     7501 tttaattcat aaataccatt aacatattga aatggtaggt taccaatagc attttttaaa
     7561 ttattttgaa attcctggct cgtatcaaca gatgaattga catcagcatg tactatcggc
     7621 gttacgtttt gcggctgcgt tggaagacca aaggtcatta cccccaacat acctaatgta
     7681 gcaattacgg ttataaacag tttatgtaat cttttcataa gctttacctc tttctaatta
     7741 cattaaacaa gcgtctacca tattgatatc cgttttagct aattgctctc cttttgtcac
     7801 aaccatatca tatgcctatt atttaagcat gtcaatcatt tatttaatct tatattaatt
     7861 taatattact ttaaatttaa tttactcgtt aaattagaaa atctgcaaat aatagctttg
     7921 ttcatcccct aacttaaatc aaaatttatg ctatgatagt ggtaaggagg cttgttctgt
     7981 gaaaaaaaca attggtatta taggtatggg agttagtggt ttagctgttc tactagcact
     8041 ttctcgtaaa aaagagaaag agttaaaaca gttagctatt ttttgttttg atgatactgt
     8101 ccactttgga cgaggaattc cttttcaaga agacgttgac tcagctttaa tcaattcccc
     8161 aatcaacgat atttcatttg attatcgcca tatgtctgac ttcatggatt ggttagaaga
     8221 aaattactat gacacaaatc taacctatgt atcaaggaca ctttacggac aatacctttc
     8281 agaaagaact aagttacttt tagataaact ccctgtcacg gttattaaaa caagagtgga
     8341 taaactaaac tatttatcag atgagcaaca atggcaagtc tatattcaag ataagctttt
     8401 tcctgtcaca tttgatgaat tacacttagc ttgtggtgct ctgcctgtta tagaacctta
     8461 tcatttggaa gggaagaccg attatatcaa agacccttat cctatcaaat acttggctca
     8521 ggaaaattgg aagggaaaaa cagttgctat catcgggact ggtctagctg caattgatgt
     8581 tataaaatgg ttgctactca atacacaatc ttctgttaaa gcattttcaa gaaccaacta
     8641 cttccctagc gtccgtattg ttaatgattt accacttact tggcactttt ttaccaatga
     8701 taaccttaaa gcatttctat cacatccttc aaaaaccttc actattgccg attttaatga
     8761 tttgatgtct ggcgaattgg agcatcttgg ctttaaaaac tggaaaaata caacagaaac
     8821 ttttttatcc ccaggaattg atggtctcaa gttagccttt aaatacccta agcaactcta
     8881 tctattacaa caattagctt cacgaatcac tgtctggtta actgaccttt ggccactcat
     8941 gtcattatca gataagaaag agtataaagc actctatgga aaagcgatta ttaacttgcg
     9001 aaatcctatg cctgatgaat ctgctaaagt aattttgaaa gctgcacaaa aagggacttt
     9061 ttctctgatc aataacgtca agacaattac aaaaaatgac gaaggatttt caatagaaaa
     9121 agaaaaacaa aatcatatat ctgtcgatcg cgtcattaac gctactggtt accagttaaa
     9181 cactaaaaac tggacaaaag ccaatccact tctaaaatcg ctcatcaatc aagaattttg
     9241 tcaaattgac gatcaaggag ggattactat tttaactgaa aatgcacaag ttttgtctcc
     9301 aaaatatggt ccaatgccag gtttatatgc gcatggtgca ctggttaatg gtgtcgttta
     9361 tcagaataac tctacaatta aaattcaaca aatggcagag cgagcctttt ctgataataa
     9421 ataaccattt ctaaaaaata cctcctcttt tattatttaa gaaagggagg tattttttta
     9481 ttataaattc ctttgttgta atatagactg tgctatactt tcttttgata attcttttaa
     9541 gttatctcct atcaattgta aagcagtatc agccatatga gcgatctctc tactatgtgt
     9601 caccataata acacatcgtt tttccttatg cgcaaaatct ttaagacgca tcgtaatatc
     9661 acgcgcaatg tcaaaatcta aatttcccgt aggttcatca agtaaaatag ctggtgttcc
     9721 tacaaccaaa gctctcgcaa tagctacacg ttgttgttgc cctcctgata atctcaagat
     9781 atttcgcgtt aacatatctt cttttaaacc taaatccaac aataattgtt tatctgcagt
     9841 aggttttacc aactgaacat tttccaatgg ggttagatag tctaaaaggt tataattttg
     9901 aaagaccaaa gctatattct ttttgcgatg ctgagcataa cctgcttctt taatatcttt
     9961 tttgttaaat aatacttgtc cctcagtagg cgaatcaaga cctgccatca aggatagaag
    10021 ggttgttttc cctgaaccag aa
//
LOCUS       DATROQ010000069         9994 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.070, whole genome shotgun sequence.
ACCESSION   DATROQ010000069 DATROQ010000000
VERSION     DATROQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9994
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.070"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(80..1912)
                     /gene="lepA"
                     /locus_tag="VBC94_001530"
     CDS             complement(80..1912)
                     /gene="lepA"
                     /locus_tag="VBC94_001530"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEO6979789.1"
                     /translation="MNIEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGFTIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRAEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGEVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGMVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYIAASIKTVAD
                     TRVGDTITLANNPAIDPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVNTTDGEMLE
                     VSNPSEFPDPTRVDSIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDDKK"
     gene            complement(2048..2464)
                     /gene="ndk"
                     /locus_tag="VBC94_001531"
     CDS             complement(2048..2464)
                     /gene="ndk"
                     /locus_tag="VBC94_001531"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEO6979790.1"
                     /translation="MEQTFFMIKPDGVKRGFIGEVISRIERRGFSIDRLEVRHADADI
                     LKRHYAELTDRPFFPTLVDYMTSGPVIIGVISGEEVISTWRTMMGSTNPKDALPGTIR
                     GDFAQAPSPNQATCNIVHGSDSPESAAREIAIWFNN"
     gene            complement(2605..2742)
                     /locus_tag="VBC94_001532"
     CDS             complement(2605..2742)
                     /locus_tag="VBC94_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979791.1"
                     /translation="MVKGLDCSTLYLIISLTALVLLVIAGVSLSLILASLQGKQWRTY
                     P"
     gene            complement(2774..3034)
                     /locus_tag="VBC94_001533"
     CDS             complement(2774..3034)
                     /locus_tag="VBC94_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979792.1"
                     /translation="MLGHRCGVLVFCYTEINFQRMWGKAVIRLIQDVIQNLVGQANEI
                     TPVYQFDWETYILATKKYERHLEVCLLVENSNYFSDSKRMCR"
     repeat_region   3087..3716
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3087..3122
                     /rpt_unit_seq="gttttggaaccattcgaaacagcacagctctaaaac"
     gene            complement(3817..4482)
                     /gene="csn2"
                     /locus_tag="VBC94_001534"
     CDS             complement(3817..4482)
                     /gene="csn2"
                     /locus_tag="VBC94_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEO6979793.1"
                     /translation="MIKINFPILDEPLVLSNATILTIEDVSVYSSLVKHFYQYDVDEH
                     LKLFDDKQKSLKATELMMVTDILGYDVNSAPILKLIHGDLENQFNEKPEVKSMVEKLA
                     ATITELIAFECLENELDLEYDEITILELIKALGVKIETQSDTIFEKCFEIIQVYHYLT
                     KKNLLVFVNSGAYLTKDEVIKLCEYINLMQKSVLFLEPRRLYDLPQYVIDKDYFLIGE
                     NMV"
     gene            complement(4469..4810)
                     /gene="cas2"
                     /locus_tag="VBC94_001535"
     CDS             complement(4469..4810)
                     /gene="cas2"
                     /locus_tag="VBC94_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEO6979794.1"
                     /translation="MSYRYMRMILMFDMPTETVEERKAYRKFRKFLLSEGFIMHQFSV
                     YSKLLLNNTANNAMIGRLKVNNPKKGNITLLTVTEKQFARMVYLHGERNTSVANFDSR
                     LVFLGDSYDQD"
     gene            complement(4807..5676)
                     /gene="cas1"
                     /locus_tag="VBC94_001536"
     CDS             complement(4807..5676)
                     /gene="cas1"
                     /locus_tag="VBC94_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEO6979795.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLVFKDSYQTEMIHLSEIDILIME
                     TTDIVLSTMLIKRLVDENILVIFCDDKRLPTAMLMPYYARYDSSLQLSRQMSWIEDVK
                     ADVWTSIIAQKILNQSFYLGECSFFEKSQSIMNLYHDLESFDTSNREGHAARIYFNTL
                     FGNDFSREHDNPINAGLDYGYSLLLSMFAREVVKCGCMTQFGLKHANQFNQFNLASDI
                     MEPFRPIVDRIIYENRQSDFVKMKRELFSMFSETYSYDGKEMYLSNIVSDYTKKVIKS
                     LNSDGNGIPEFRI"
     gene            complement(5678..9790)
                     /gene="cas9"
                     /locus_tag="VBC94_001537"
     CDS             complement(5678..9790)
                     /gene="cas9"
                     /locus_tag="VBC94_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040090.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEO6979796.1"
                     /translation="MNKPYSIGLDIGTNSVGYSVVTDDYKVPAKKMRVLGNTDKEYIK
                     KNLIGALLFDGGNTASDRRLKRTARRRYTRRRNRILYLQEIFAEEMSKVDDSFFHRLE
                     DSFLVEDDKRGSKYPIFATMQEEKDYHEKFPTIYHLRKELADKKEKADLRLVYLALAH
                     IIKFRGHFLIEDDSFDVRNTDIQRQYQAFLEIFDTTFENNHLLSQNIDVEGILTDKIS
                     KSAKKDRILAQYPNQKSTGIFAEFLKLIVGNQADFKKHFNLEDKTPLQFAKDSYDEDL
                     ENLLGQIGDEFADLFSVAKKLYDSVLLSGILTVTDLSTKAPLSASMIQRYDEHREDLK
                     QLKQFVKASLPEKYQEIFTDSSKDGYAGYIEGKTNQGAFYKYLSKLLTKQEGSEYFLE
                     KIKNEDFLRKQRTFDNGSIPHQVHLTELKAIIRRQSEYYPFLKENLDRIEKILTFRIP
                     YYVGPLAREKSDFAWMTRKTDDSIRPWNFEELVDKEASAEAFIHRMTNNDLYLPEEKV
                     LPKHSLIYEKFTVYNELTKVRYKNEQGETYFFDSNIKQEIFDGVFKEHRKVSKKKLLD
                     FLAKEYEEFRIVDVIGLDKENKAFNASLGTYHDLKKILDKDFLDNPDNESILEDIVQT
                     LTLFEDREMIKKRLENYKDLFTESQLKKLYRRHYTGWGRLSAKLINGIRDKESQKTIL
                     DYLISDGRANRNFMQLIHDDGLSFKPIIDKAQAGSHSDNLKEVVGELAGSPAIKKGIL
                     QSLKIVDELVKVMGYEPEQVVVEMARENQTTNQGRRNTRQRYKLLEEGVKNLASDLNG
                     NILKEYPTDNQALQNERLFLYYLQNGRDMYTGEALDIDNLSQYDIDHIVPQAFIKDDS
                     IDNRVLVSSAKNRGKSDNVPSIDIVKARKAFWKKLLDAKLMSQRKYDNLTKAERGGLT
                     SDDKARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIRKVKIVTLKSNLVSNFR
                     KEFGFYKIREVNDYHHAHDAYLNAVVAKAILTKYPQLEPEFVYGDYPKYNSYKTRKSA
                     TEKLFFYSNIMNFFKTKVTLADGTVVVKDDIEVNNDTGEIVWDKKKHFATVRKVLSYP
                     QVNIVKKTEIQTGGFSKESILAHGNSDKLIPRKTKDIYLDPKKYGGFDSPIVAYSVLV
                     VADIKKGKAQKLKTVTELLGITIMERFRFEKNPSAFLESKGYLNIRDDKLMILPKYSL
                     FELENGRRRLLASAGELQKGNELALPTQYMKFLYLASRYNELKGKPEEIEQKQEFVVQ
                     HVSYFDDILQIINDFSNRVILADANLEKINKLYQDNKENISVDELANNIINLFTFTSL
                     GAPAAFKFFDKIVDRKRYTSTKEVLNSTLIHQSITGLYETRIDLGKLGED"
BASE COUNT         3152 a         1991 c         1517 g         3334 t
ORIGIN      
        1 taattgcaga caaagaaaaa gtaacttaag cacgaagcta agttacttta aaatagataa
       61 atgatatcat ctttctgaat tattttttgt catcatcgtc cattgataaa acacttaaga
      121 aagcttcctg aggaacttcg acggaaccaa ttgctttcat acgcttttta ccagcttttt
      181 gtttttcaag caacttacgt ttacgtgaaa cgtcaccacc ataacatttt gctaagacat
      241 ttttgcgtag tgcttttata tcagaacgag ccacaatttt ttgtccaata gctgcttgga
      301 taggaacttc aaactgctga cgaggaatga tttttttcaa cttatcaacg atcaatttac
      361 cacgttcata ggcaaattct ttgtgaacaa taaagcttaa agcatccact ttatcaccat
      421 ttaaaagaat atccatttta actaactgtg agcgacgata ttcggaaatt tcataatcaa
      481 aactagcata gccacgtgtt gatgatttaa gcttatcaaa gaagtcaaac acaatttcag
      541 ctaaaggaat ttggtaaata acattaacac gattatcatc aatataatcc attgttacaa
      601 agtcaccacg tttacgttgt gctaactcca ttacagcacc gacaaattct tgaggaacca
      661 taatttgcgc cttaacatat ggttcttcaa tgctgtcaac acgagtagga tcaggaaatt
      721 cagatggatt cgaaacttca agcatttcac catcagttgt attaacatga taaactactg
      781 atggtgcagt cataataaga tcaatattga actctcgttc caagcgctct tggataacgt
      841 ccatatgtaa aagtcctaag aaaccacaac ggaaaccaaa ccctaatgct tgtgatgttt
      901 ccggttcaaa ctgtagacta gcatcattca attgtaattt ttcaagggct tcacgcagat
      961 cgttatactt atttgattca attggataaa gaccggcaaa aaccattggg ttcatttgct
     1021 tatagccatg caaggggtct atagctgggt tattagcaag ggtaatggta tcaccaacac
     1081 gtgtatcagc aactgtttta attgaggcag caatataccc tacatctcca gttgctaaaa
     1141 aatcacgtcc aactgctttg ggagtgaaaa taccaacctc agtaacgtcg aaggttttac
     1201 cattagacat catttgaatc ttatctcctg gcttaaccat accatttaca atccttactt
     1261 gcagaattac tccacgataa gcatcgtaaa cagaatcaaa aattagagct tgaagaggag
     1321 catctacttc cccagtcgga gcaggaactt tttcaacgat ctgctctaaa atttcttcaa
     1381 taccaatccc tgccttagct gaggcaagta cagcttctga agcatctaga ccaataacat
     1441 cctctacttc tgcacgtaca cgttctggat ctgctgctgg taaatcaatt ttattaataa
     1501 ctggtagtat ctctaaatca ttgtctagag ccaaataaac atttgccaga gtttgagctt
     1561 caattccttg ggctgcatca acaaccagta tagcaccctc acaagctgcc aatgaacgtg
     1621 atacttcata tgtaaagtca acatgccctg gcgtgtcaat caagtgaaaa atgtaagttt
     1681 caccatcttt agcagtataa ttaagctcaa tagcatttaa ctttatagta aacccacgct
     1741 cacgttctag gtccatagag tccaacagtt gcgcctgcat ttcacgacta gaaaccgttt
     1801 ctgttttctc taaaatacgg tctgctaagg ttgattttcc atggtcaata tgagcgataa
     1861 tcgaaaagtt tctaatcttt tcttgacgtt tcttcaaatc ttcaatattc attctatttc
     1921 ctatacaatt tgatatgttt aacttctaaa atattaaaac agtcctatag actctagaat
     1981 attatatcaa aaagaagacc gatgtaaaag agaattaaca cttgaacatc aatcttcatt
     2041 aagtctatta gttattgaac caaatggcta tctcgcgagc tgcagattca ggtgagtctg
     2101 acccatgaac aatattacat gtcgcttggt ttggagaggg agcttgtgca aaatctcctc
     2161 gaatagttcc tggaagggca tctttggggt tagtactccc catcatggtg cgccaagttg
     2221 aaatgacctc ttctcccgaa ataactccaa tgattactgg accactagtc atataatcta
     2281 ctaatgttgg aaagaatggt ctatccgtca gttctgcata atgtcttttc aaaatatctg
     2341 catctgcatg tctaacctct aagcgatcaa tagagaagcc tcgacgctca atacgactaa
     2401 taacttctcc aataaaacca cgttttacac catcaggttt tatcataaaa aaggtttgtt
     2461 ccataatatt agcacctcat ttctttctcc actattataa catgaaaata tgttttctgt
     2521 aacttccaac tgtatttcaa aaaaggagtg tcggacactc cttaactata ttaataggtg
     2581 agatccatca tagctgttag ttgattatgg ataggttctc cactgctttc cttgtaagct
     2641 tgcaagtatt agtgacaaag aaacaccagc aatcactaaa agaaccaacg ctgtcaaact
     2701 aataattaag taaagcgtag aacaatctag ccctttaacc ataaggttgg aattatagtc
     2761 tttcgtatct cttctatcga cacattctct ttgaatccga aaaataattc gaattttcta
     2821 ctaatagaca cacctctaaa tgacgttcat attttttagt cgccaatata taagtttccc
     2881 aatcaaactg ataaactggg gtgatttcgt tagcttggcc aactaagttc tgaattacgt
     2941 cttgaattaa tctaattacc gccttccccc acattctctg aaaatttatt tcagtataac
     3001 aaaaaactag aacaccacaa cggtgcccta gcataataac cgcacagctc taaaacacat
     3061 atcaggaata taaaaatcgt tcaaatgttt tggaaccatt cgaaacagca cagctctaaa
     3121 actgcctttt aattcagctc ccacttttta tcgttttgga accattcgaa acagcacagc
     3181 actaaaaccg atttgctcgt acattataaa taccaccagt tttggaacca ttcgaaacag
     3241 cacagctcta aaactttatt ttttctcagt tccttgattt tagagttttg gaaccattcg
     3301 aaacagcaca gctctaaaac taaatacttt agaagaaaga gttggttttt gttttggaac
     3361 cattcgaaac agcacagctc taaaactcag aaaggttgat agaataatgt ttccaagttt
     3421 tggaaccatt cgaaacagca cagctctaaa acaccaaaat aagtctttaa aattgctttg
     3481 gcgttttgga accattcgaa acagcacagc tctaaaacta gaaatcctta ggcgttcctc
     3541 ttaaagtagt tttggaacca ttcgaaacag cacagctcta aaacttttcc aatcacggtt
     3601 catgttccaa cggtgttttg gaaccattcg aaacagcaca gctctaaaac tttgcttcta
     3661 aaaaaaatac agatgaagat gttttggaac cattcgaaac agcacagctc taaaacctcg
     3721 tagaaaattt tcttttgagc tttcgtaatc gcgccattcg tctcagcagg acttcagttt
     3781 cgatgattcc ttgttattac tgtgctttta ctaatattat accatatttt cgcctatcaa
     3841 gaaataatcc ttatcaataa catattgcgg taaatcatag agtcttctag gttctagaaa
     3901 gagtactgac ttttgcatta aattgatgta ttcacataat tttataactt catctttggt
     3961 aagataagct ccgctattaa caaaaaccaa gagattcttt ttcgttaaat aatggtaaac
     4021 ttgtataatt tcaaaacatt tttcaaagat agtgtcgctc tgtgtctcaa ttttgactcc
     4081 tagtgcctta atgagttcta aaatcgtgat ttcatcgtat tctaaatcaa gctcattctc
     4141 tagacactca aatgcgataa gttctgtaat agtagctgct aatttttcta ccattgattt
     4201 cacttctggc ttttcgttga attgattttc taagtcacca tgtatcaact ttagaatagg
     4261 tgctgagttg acatcgtatc ctaagatatc tgtaaccatc attaactctg ttgccttcag
     4321 acttttctgc ttatcatcaa ataatttcaa atgttcatct acgtcatatt gataaaaatg
     4381 tttcaccaat gaagaataaa ctgaaacatc ttctatcgtt aaaatcgtag cattacttaa
     4441 cactaatggt tcatctaaaa ttggaaaatt aatcttgatc ataagaatct cctaggaaaa
     4501 ccaagcgact atcaaagttg gcaacacttg tgttgcgttc tccatggagg taaaccattc
     4561 tcgcaaattg tttttccgta actgttaaga gtgtgatatt acccttctta ggattattca
     4621 ctttaagccg acctatcata gcattattag ctgtattatt gagtaataat ttactataaa
     4681 cagagaactg atgcataata aaaccttcgc tcaaaagaaa ctttctaaac ttacgatacg
     4741 ccttacgttc ttctactgtt tcagtaggca tatcaaacat taaaatcatt ctcatatacc
     4801 gataactcat atcctaaact ccggaatccc attcccatca ctatttagcg acttaataac
     4861 ttttttggtg taatcgctga caatatttga aagatacatt tctttaccgt cgtaactgta
     4921 tgtctctgaa aacatagaaa agagttctct tttcattttg acaaaatcac tctgcctatt
     4981 ttcataaata atcctatcaa cgattgggcg aaatggttcc ataatatcgc ttgctaggtt
     5041 gaactgatta aattgattag catgctttaa cccaaattgt gtcatgcaac cacacttaac
     5101 gacttcacgc gcaaacatac tcaaaagcaa tgaatatccg tagtctaaac cagcatttat
     5161 tggattatca tgttctcttg aaaaatcatt tccaaaaagt gtattgaaat aaatcctagc
     5221 tgcatgccct tcacgattgg aagtatcaaa agattctaaa tcatgataga gattcataat
     5281 agactgggat ttttcaaaga aagaacactc accaagataa aaagactgat tcaaaatttt
     5341 ttgtgcaatg attgacgtcc aaacatccgc tttgacatct tcaatccatg acatctgcct
     5401 agataattgt aaactcgaat catatctggc atagtacggc attaacatag ctgttggcaa
     5461 gcgtttgtca tcacaaaata taactaaaat attttcatca accaaacgtt taatcagcat
     5521 ggtcgacaaa acgatatctg ttgtttccat gattagaatg tcaatctctg atagatgaat
     5581 catttccgtc tgataagaat ctttaaaaac cagatgatta tttttataag agagcttaga
     5641 atgtgtattt acaacaacgg ttcgccaacc tgccatatca atcttctcct aacttaccca
     5701 aatcaatacg tgtttcataa agtccagtaa tagattgatg aattagggta gaattaagta
     5761 cttctttagt tgatgtatag cgttttctat caactatttt atcaaaaaat ttaaaagctg
     5821 ctggagctcc tagactggta aaagtaaata gattgataat attattagca agttcatcta
     5881 ctgatatatt ttccttatta tcttggtaaa gcttattgat tttctctaaa ttagcatctg
     5941 ctagaataac tcgatttgaa aaatcgttga ttatttgaag aatatcgtcg aaataagaaa
     6001 catgttgaac tacaaattcc tgtttttgtt caatttcttc tggttttcct tttagctcat
     6061 tgtaacgact tgcaaggtat aagaacttca tatattgtgt tggtaaggct agctcgttac
     6121 ctttttgtaa ttcaccagca ctagcaagta atcgacgacg cccattttct aattcgaaca
     6181 gactatactt cggtaaaatc attaatttat cgtccctaat atttaaataa ccttttgatt
     6241 caaggaaagc tgatggattt ttctcaaatc tgaatctctc catgatggta attcctaaaa
     6301 gttccgtaac tgtttttagt ttttgtgctt tacctttttt gatatcagct acaactaaaa
     6361 cagagtaagc tactatcgga ctatcaaaac ctccatattt cttaggatct aaataaatat
     6421 ccttcgtttt tcttggaatc aacttatctg agttaccatg cgccaagatt gattccttag
     6481 agaaaccacc tgtctgaatc tctgtcttct tcacgatatt gacctgaggg tatgacaaga
     6541 cttttctaac tgtcgcaaag tgtttcttct tatcccaaac aatttcaccc gtatcattat
     6601 taacttcaat atcatctttt acaacaacgg ttccatccgc caaagttacc ttagttttaa
     6661 agaagttcat aatatttgaa tagaaaaata gcttttctgt agcggattta cgcgttttgt
     6721 aactgttata ttttggatag tcaccgtaga caaattctgg ctctaactga ggatatttgg
     6781 ttagaatagc tttagcaact actgcattaa gataggcatc atgtgcatgg tgataatcat
     6841 taacttcacg aattttatag aatccaaatt cttttcggaa atttgaaacc aaatttgact
     6901 tcaaggttac aattttaact ttgcgaatcc ttctaccttt actatcaagc tcattattaa
     6961 agcgttcatc caagatacgg gcaacatgct tggtaatttg tcgtgtctca accaactgac
     7021 gttggataaa tcttgcctta tcatcggaag ttaggcctcc gcgctctgcc ttagtcaaat
     7081 tatcatactt acgctgactc attaacttag catcaagtaa ttttttccag aatgctttgc
     7141 gtgcttttac aatatcaatg ctaggaacat tatctgattt tccacggttt ttagcagatg
     7201 ataccaaaac acgattatca atagaatcat cttttatgaa agcttgagga acaatgtgat
     7261 caatatcata ttgacttaaa ttgtcaatat ctagtgcttc ccctgtgtac atatctcttc
     7321 cattttgtaa gtaataaagg aaaagtcttt cattttgcaa cgcttgatta tccgtaggat
     7381 attctttcaa aatattgcca ttcaagtcac tagctagatt cttaacgcct tcctcaagaa
     7441 gtttatagcg ttgtcgagtg ttacgacgac cttgatttgt tgtttgattc tcacgcgcca
     7501 tctcaaccac aacttgttca ggttcgtatc ccatgacttt aacaagctca tcaacaattt
     7561 tcaaactttg tagaatcccc tttttaatag cagggctacc tgcaagctca cctacaactt
     7621 cttttagatt atctgaatga ctaccagcct gtgccttgtc aataattggt ttaaatgata
     7681 gaccatcatc atgtatcaac tgcataaagt tgcgatttgc tcttccatca gaaatcagat
     7741 agtccaagat tgttttttga ctctctttat ctctaatgcc attaattaac ttagcagaca
     7801 atcgtcccca gccagtatag tgacggcgat agagtttttt tagttgtgac tctgtaaaaa
     7861 gatctttata gttttcaaga cgcttcttaa tcatttctct atcttcaaat aaggttagag
     7921 tttggacgat atcttccaaa atagactcat tatctggatt atcaagaaaa tctttgtcta
     7981 gtattttttt gagatcgtgg taagttccca atgaggcgtt gaaagcttta ttttctttat
     8041 ctagaccaat aacatctact atcctaaact cctcatattc tttagccaga aaatctagca
     8101 acttcttctt ggatacctta cgatgttcct tgaatactcc atcaaagatt tcttgtttaa
     8161 tattgctatc aaaaaaataa gtctcacctt gctcattttt atatctaacc ttagtcaact
     8221 cattatagac cgtaaatttt tcataaataa gactatgttt tggtaggact ttttcttcag
     8281 gaagatagag gtcattgttg gtcatacgat ggataaaagc ttccgcagat gcttccttat
     8341 caaccaattc ttcaaaattc caaggtcgaa tactgtcatc tgttttgcga gtcatccatg
     8401 caaaatcact cttctcacgt gctagtggcc cgacataata aggaattcta aaggtaagga
     8461 ttttttcaat cctatctaga ttctctttca agaatggata gtattctgat tgacgtcgaa
     8521 taatagcctt caattctgtc aaatggactt ggtgtggaat tgagccatta tcaaaggtcc
     8581 tttgttttct caaaaaatct tcattcttga ttttttcaag aaaatactcg ctaccttctt
     8641 gcttggtcaa caattttgac aagtatttat aaaatgctcc ttgattagtt ttgccttcaa
     8701 tataaccagc gtagccatct tttgatgaat cagtaaatat ttcttgatat ttctcaggca
     8761 atgaagcttt tacgaattgt tttaactgtt tcaagtcctc tctatgttca tcgtaacgct
     8821 gaatcataga ggcagaaagt ggcgccttgg tactgagatc agttactgta agaatgccag
     8881 ataaaagaac actatcatat agctttttcg ctactgagaa taagtcagca aattcatcac
     8941 caatctgccc aagaagattt tctaaatctt catcgtagct atccttagca aattgaagcg
     9001 gcgttttatc ttccaaattg aaatgtttct tgaagtcagc ttgatttccg acaatcaatt
     9061 tcaaaaattc tgcaaaaatg ccagtagatt tttggttagg gtactgcgct aaaatgcgat
     9121 ctttcttcgc agacttgcta atcttatctg ttagaattcc ctctacatct atgttttgag
     9181 ataacaaatg gttattttcg aaggtagtat caaagatttc taaaaaagct tgatattgtc
     9241 tttgaatatc agtattcctc acatcaaagc tatcatcctc aattaggaaa tgtcctctga
     9301 atttaatgat atgagctaga gccagataaa caagacgaag gtctgctttt tctttcttgt
     9361 cagctaattc ttttctcaag tgataaatcg ttgggaactt ttcatgataa tctttctcct
     9421 cctgcattgt tgcaaagata ggatacttgc tccctctctt gtcgtcctca actagaaaag
     9481 aatcctctaa tcgatgaaag aaactatcat caactttact catttcctct gcaaaaattt
     9541 cttgtaaata tagaatacga tttctacgac gtgtataacg acggcgagca gttcgcttca
     9601 agcgtctatc tgaagcagta tttccaccat caaaaagcag agcacctata agattcttct
     9661 ttatgtattc tttatctgta ttccctaaga ccctcatctt cttagcaggt accttataat
     9721 catctgtaac cacagaatat ccgacggaat tagttccgat gtcgagacct attgaatatg
     9781 gtttgttcat aatttatctc cttttattta aaatatcact ttaaaaacgc aaactttaaa
     9841 gttttgctaa tttttacttg attattacat taaaataaca tataataatt ttgttggaat
     9901 tattcgaaac agcacagcac gttaaaataa ggcagtgatt ttttaatcca gtccgtattt
     9961 agcttgaaaa agtgagcacc gattcggtgc tttt
//
LOCUS       DATROQ010000070         9896 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.071, whole genome shotgun sequence.
ACCESSION   DATROQ010000070 DATROQ010000000
VERSION     DATROQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.071"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(86..871)
                     /locus_tag="VBC94_001538"
     CDS             complement(86..871)
                     /locus_tag="VBC94_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEO6979797.1"
                     /translation="MTYTSLKSPTTKDYKYTLNVAHLYGNLLNTYGDNGNILMMKYVG
                     EKLGCQMTFDIVSLEDRFDPNYYQMAFFGGGQDYEQAIVARDLPSKKEDINKFIQNNG
                     VVLAICGGFQLLGQYYIQANGERIEGIGVMGHYTLNQNNNRYIGDIKIHNDEFNETYY
                     GFENHQGRTFLSEDEKPLGTVIYGNGNNKEDGTEGVHYKNVFGSYFHGPILSRNANLA
                     YRLVATALRNKYGKEIVLPSYEEILSLEIPEEYGDVKSKADFE"
     gene            complement(871..2214)
                     /locus_tag="VBC94_001539"
     CDS             complement(871..2214)
                     /locus_tag="VBC94_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEO6979798.1"
                     /translation="MKINTALGVAAGKSAHYLLSKMGRGSTYPGSLALKFDKDILDTI
                     AKDYEIVVVTGTNGKTLTTALTVGILKEAFGQVVTNPSGANMITGIVSTFLTAKKSKS
                     GKKIAVLEIDEASLPRITQYIKPSLFVFTNIFRDQMDRYGEIYTTYQMILDGAANAPQ
                     ATILANGDSPLFNSKSVTNPVQFYGFDTDKHEPRLAHYNTEGILCPKCQAILTYRLNT
                     YANLGDYTCPNCDFERPNLDYALTRLIHLTNTSSGFVIDGQQYNINVGGLYNIYNALA
                     AVSVAEYFGVEASQIKDGFDKSRAVFGRQETFTIGNKKCTLVLIKNPVGASQALDMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDANFETILTMNIPEIFAGGVRHSEIARRLRVT
                     GYDEKRIKQADKLQDIMAMIEQQETEHAYILATYTAMLEFREILANHNAIRKEMN"
     gene            2354..3205
                     /locus_tag="VBC94_001540"
     CDS             2354..3205
                     /locus_tag="VBC94_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEO6979799.1"
                     /translation="MDIFSAIDSKFWASIMENPWMILIHLMDIIIVAVLIYKFIKALA
                     GTKIMSLIQGVILFIIIRFVSEWIGLTTITFLMNQIVTYGVIAGVVIFAPEIRTGLEK
                     FGRTPQLFTQRSQLSSDEKLVDALVKAVAYMSPRKIGALISIERTQTLQEYIATGIPL
                     DADISSELLINIFIPNTPLHDGAVIVKDKKIATACSYLPLSESSSISKEFGTRHRAAI
                     GLSENSDALTVIVSEETGGISVALKGEFLHDLSKDSFEAILRTQLIQNQEENSKLAWY
                     NQLLRRK"
     gene            3208..4167
                     /locus_tag="VBC94_001541"
     CDS             3208..4167
                     /locus_tag="VBC94_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEO6979800.1"
                     /translation="MKKFFTNKFWLGVVSLFLAILLFLTATATSMNHQDNSKIAGASE
                     TYTHTLTDVPIDIKYDSDDYFISGYSYGADVYMSSVNRVKLDSEINEDTRKFKVVADL
                     TNMKPGTHKVPLKVVNLPSGVNATVSPTTITVTMGKKKTKEFPVYGHVNDKQIKAGYA
                     VDKMSVDVSKVKVTSDESIIDRIDHVAANIPDDKVLDDDFNKTVTLQAVTADGTVLAS
                     IIHPSKATLSVKVKKLTKTVPINLIPVGQFSDSISKINYKLSQEKAVISGTKEALEAI
                     SVINAEVDISDVTKNTEKKINLSANNVSVDPAQVTVQLTTTKK"
     gene            4221..5573
                     /locus_tag="VBC94_001542"
     CDS             4221..5573
                     /locus_tag="VBC94_001542"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEO6979801.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETDRPRV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGSDGFKLDDDRELEIEALLDAKEDTLPRPSAQGLGTLVDYPEGLRKY
                     EKFMESTGIDLEGMNVALDTANGAATASARNIFLDLNADISVIGDQPDGLNINDGVGS
                     THPEQLQSLVRENGSDIGLAFDGDSDRLIAVDENGEIVDGDKIMFIIGKYLSDKGQLA
                     QNTIVTTVMSNLGFHKALDREGIHKAITAVGDRYVVEEMRKSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLTKVMKETGKKLSELASEVTVYPQKLVNIRVENNMKDKAMEVPAI
                     AEIIAKMEEEMDGNGRILVRPSGTEPLLRVMAEAPTNEAVDYYVDTIADVVRTEIGLD
                     "
     gene            5696..6067
                     /locus_tag="VBC94_001543"
     CDS             5696..6067
                     /locus_tag="VBC94_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEO6979802.1"
                     /translation="MTKEAELLWAKHKYLVLSKSQKIYLDIRQTLKSPNCTVLDVQSL
                     IDQAVLLEESPSQVTNAYMHIWGYFKNKAERQEKEEFLTLLEKYRKTGYQRRKLLAFL
                     KQLLAKYPNSYLQNSSIFEEE"
     gene            6092..6472
                     /locus_tag="VBC94_001544"
     CDS             6092..6472
                     /locus_tag="VBC94_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979803.1"
                     /translation="MRLWHQDIIELLPRQQLLGQHRECCALRGNGWGRKHETVDYVFR
                     YSPYRLFAYHQLVMEEMMERGYRVSKEWLIAEYRGMKCPRYDTLNPVDLETPIYPEHN
                     QDYLQECLWNLKAKGIDLPINKIK"
     gene            6565..7695
                     /locus_tag="VBC94_001545"
     CDS             6565..7695
                     /locus_tag="VBC94_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEO6979804.1"
                     /translation="MLKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDAYLQALIRE
                     FRSYDITELRTLYIGGGTPTSISAVQLDYLLTELSRDLNLNTLEEFTIEANPGDLTVD
                     KIEVLQKSAVNRVSLGVQTFNDKHLKRIGRSHNEVQIYSTIDALKTAGFQNISIDLIY
                     ALPGQTMDDVRSNVAKALSLNIPHLSLYSLILEHHTVFMNKMRRGKLHLPTEDLEAEM
                     FEYIISEMERNGFEHYEISNFTKPGFESRHNLMYWDNVEYYGVGAGASGYLDGIRYRN
                     RGPIQHYLKGVSEGNARLSEEVLSKNEMMEEELFLGLRKKKGVSIGKFEQKFGTSFEK
                     RYGQIVQELQSDGLLKENNGFIQMTKKGLFLGDTVAEKFIVE"
     gene            7699..8436
                     /locus_tag="VBC94_001546"
     CDS             7699..8436
                     /locus_tag="VBC94_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEO6979805.1"
                     /translation="MGLLYRETYEVPFYESDTNHYMKLPQLLALALQISAKQSLKLGI
                     GDDIVFKRYGLVWVVTDYIIDIERLPKHAEKIVIETEAKAHNKLLCYRYFYIYGEDGQ
                     KIITISSAFVLMDFKTRKIHPVLDDITSIYQSQRIKKVIRGPKYHPIGDSKVKQYHVR
                     YFDLDMNGHVNNSKYLEWMYDVLDLDFLSSHIPKKIDLKYIKEIQYGTDIKSHWYQDG
                     LVTRHDIIGGDAIHAQARIEWQEKKED"
     gene            8437..9207
                     /locus_tag="VBC94_001547"
     CDS             8437..9207
                     /locus_tag="VBC94_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEO6979806.1"
                     /translation="MAYKGYLIDLDGTIYKGKSRIPAGERFIERLQEKGIPYMLVTNN
                     TTRTPESVQEMLRGFNVETPLETIYTATMATVDYMNDMNRGKTAYVIGEEGLKKAIAD
                     AGYVEDTKNPAYVVVGLDWNVTYDKLATATLAIQNGALFIGTNPDLNIPTERGLLPGA
                     GSLNALLEAATRIKPVFIGKPNAIIMNKALEILNIPRNQAVMVGDNYLTDIMAGINND
                     IDTLLVTTGFTTVEEVPDLPIQPSYVLASLDEWTFNEG"
     gene            9197..9853
                     /locus_tag="VBC94_001548"
     CDS             9197..9853
                     /locus_tag="VBC94_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEO6979807.1"
                     /translation="MKDKLLVVLTWIWIISLATLATIYIAWLIYPIEIQFLKLEKVVY
                     LKAETIYYNFNKLMIYLTHPFISDLNMPSFPSSEDGLKHFADVKYLFTLAHGLFVILT
                     FPVIYFLRRGWKQKSIFLYEGFFKIAIMLPIFIVVCAFLLGFDQFFTLFHEVLFPGDS
                     TWQFNPLTDPVIWILPETFFLHCFIIFLLIYETITIILLIIGRKHLKLRKYKNKMQAL
                     "
BASE COUNT         3236 a         1530 c         1940 g         3190 t
ORIGIN      
        1 atgcgttaaa agttagttga accgccgtat gccgaacggc acgtacggtg gtgtgagagg
       61 ggctagagat tagcctctac tcgatttatt caaaatctgc ttttgattta acgtctccgt
      121 attcctcagg aatctctaaa gataaaattt cttcataact agggaggaca atctctttac
      181 catatttgtt acggagggca gttgccacaa gacggtaagc tagattagca ttacgtgaaa
      241 ggattggacc gtgaaagtat gacccaaaaa cattcttata atgaacccct tcagtaccat
      301 cttctttgtt attcccattt ccataaatga cagttcctaa aggtttttcg tcttctgata
      361 aaaaggtacg tccttgatga ttctcaaagc cataatatgt ttcattgaat tcatcgttgt
      421 gaatcttgat atcaccaata tagcgattat tattttggtt tagcgtatag tgtcccataa
      481 cgccaatgcc ctctattcgt tctccattag cttgaatata gtattgtcct aaaagttgga
      541 aacctccaca aatagcaagt actacaccat tattttgaat gaatttgtta atgtcttctt
      601 tcttactggg aagatcgcgt gcaacaatag cttgttcgta gtcttgtcca ccaccaaaaa
      661 atgccatctg ataatagttg ggatcaaatc tatcttctaa tgaaacgata tcaaatgtca
      721 tttgacatcc tagtttttct cccacatatt tcatcattaa gatattccca ttatcgccat
      781 aagtatttaa taggttacca taaagatgag ctacattaag agtgtattta taatctttgg
      841 tagtaggaga ttttagtgag gtataagtca ttaattcatc tcctttctga tagcgttgtg
      901 gttagcaaga atttcacgaa actccagcat agctgtatat gttgctaaaa tataagcatg
      961 ctcagtctct tgctgctcaa tcattgccat aatatcttgc agtttatcag cttgttttat
     1021 gcgtttctca tcataaccag ttacacgtaa gcgacgagca atttcagagt ggcgaacacc
     1081 accagcaaat atttctggga tattcatcgt gagaatagtt tcaaaattag cgtcccaaat
     1141 ccaagaagta tcaataccgt cagcataatt ggcatttaat agtactgaaa gagagaacgg
     1201 gtatggagca agtttaatca tatcaagagc ttgactagct cctactggat ttttaatcag
     1261 aactaaggta cattttttat ttccaattgt aaaggtttct tgacgaccaa aaacggcacg
     1321 gcttttatca aatccatctt taatttgact tgcttctaca ccaaaatatt cagcaacaga
     1381 tactgccgcc aaagcattgt aaatattata aaggccaccc acgttgatat tatattgttg
     1441 gccatcaata acgaaacctg atgaagtatt tgtcagatgt attagccttg ttaaggcata
     1501 atcaaggttt ggacgttcaa aatcacaatt aggacaagtg tagtcaccaa ggttggcata
     1561 tgtgttcaat cgataagtta aaattgcttg acactttgga cagagaatac cctcagtatt
     1621 atagtgtgct aatcgtggct catgtttgtc ggtatcaaat ccatagaatt gcacagggtt
     1681 agtcacgctt ttagaattaa aaagtggact gtcaccgtta gctaagatag tagcttgcgg
     1741 agcgttagca gctccatcta aaatcatttg ataagttgtg tagatttcgc cataacggtc
     1801 catttgatca cggaaaatat tggtaaagac gaacaaacta ggctttatat actgtgtaat
     1861 acggggtaaa ctcgcttcgt ctatttcaag gacagctatt tttttaccag atttactttt
     1921 cttagctgtt aggaaggttg aaacaatacc tgtaatcata tttgctccac ttgggttggt
     1981 taccacctga ccgaaggctt cctttaaaat accaactgtt aaagctgttg ttaaggtttt
     2041 accgttagta ccagtcacaa ctacaatttc ataatctttg gcaattgtat ctagtatatc
     2101 tttatcgaat ttgagtgcta aactacccgg ataagtagaa ccacgtccca ttttacttaa
     2161 aaggtaatga gcagacttac cggcagccac tcctagtgct gtatttattt tcataaagat
     2221 attttatcat aaaaagatgc tttcttgcta aagaaaatat tatgaaaaca tggtataatg
     2281 tagacgatgg aaaataagaa atcaaggagt taagcaagaa actcttcgac aggctaccag
     2341 tagagaggaa gatatggata ttttttcagc tattgattca aagttttggg cgagcattat
     2401 ggaaaatcca tggatgatcc ttattcatct catggatatt atcatagtag ctgttctgat
     2461 ttataaattt atcaaagcgc tagcaggcac aaaaattatg tccttaattc agggcgttat
     2521 tttgtttatc atcattagat ttgtgtcaga atggataggg ctaacaacga ttacattttt
     2581 gatgaatcag attgttactt atggtgtaat tgcaggtgtt gtcatctttg ctccagagat
     2641 tagaacaggt ctcgaaaaat ttgggcgaac accacaacta tttacgcaac gttctcaatt
     2701 aagcagtgat gaaaagctag tagatgccct tgttaaggca gttgcttata tgagtccgcg
     2761 taaaatagga gccttgattt caattgaacg tacccaaact ttgcaagagt atatagctac
     2821 aggtatccca ttagatgcag atatttccag tgaattgtta attaatattt tcattcctaa
     2881 tacaccactg cacgatggtg ctgttattgt aaaagataaa aaaattgcta cagcatgttc
     2941 gtacttacct ctatcagaat catcttcgat ttcaaaagaa tttggaacac gccacagagc
     3001 agcgattggt ctatcagaga attcagatgc actaacggtt attgtttctg aagagactgg
     3061 tggtatttct gttgctttga aaggtgaatt tttacatgat ctttctaaag atagctttga
     3121 agcaattctg agaactcagt tgattcaaaa tcaagaggaa aactcaaaat tagcttggta
     3181 taatcaacta ttgaggagga agtagacatg aaaaaatttt ttacaaataa attttggtta
     3241 ggcgttgtct cactctttct ggctatttta ttatttttaa cggcaactgc aacaagtatg
     3301 aaccatcaag acaatagtaa gattgcagga gcgtctgaga catatacaca taccctaaca
     3361 gatgtgccaa tagacatcaa atacgatagt gatgattact ttattagtgg ctattcttat
     3421 ggggcagatg tctatatgtc atcggttaat cgtgttaagc tagattctga aataaatgag
     3481 gatacacgaa aatttaaagt agtcgctgat ttgacaaata tgaaaccagg gactcacaaa
     3541 gtcccactaa aggtagtaaa tttgccatct ggtgtaaatg ctacggtttc tccaacaaca
     3601 attacagtaa ctatgggtaa aaagaaaaca aaagagtttc cggtttatgg acatgttaat
     3661 gataagcaaa ttaaagctgg ttatgcagtc gataagatgt cagttgatgt gtcaaaagtt
     3721 aaagttacta gtgatgaatc tattatagat cgtattgacc atgtcgctgc caatatccct
     3781 gatgataaag ttcttgatga tgattttaac aagacagtta ctttacaagc tgtaacagca
     3841 gatggtacag tattagcaag tattattcac ccttctaaag caactttatc tgtcaaggta
     3901 aaaaaattga ccaagacagt accgattaat ttgataccag taggacaatt cagcgatagt
     3961 atttcaaaaa ttaactacaa gttgagtcaa gaaaaagcag taatttcagg aacaaaagag
     4021 gctttagaag ctatttctgt tattaatgct gaggtagata tttcagacgt aactaaaaat
     4081 acagaaaaga aaattaatct ttctgcaaac aatgtttcag ttgatcctgc tcaagtaaca
     4141 gttcaattga caactactaa aaaataatag ataataaaaa cgaaaaaaat ggaggtagcg
     4201 tttttcgcta gaaaaaaatt atgggaaaat attttggtac agatggtgtt cgcggtgaag
     4261 ctaatgttga actgacgcca gaattggctt ttaaattagg acgttttgga ggatatgttc
     4321 taagtcaaca cgaaactgat cgtccgagag tttttgtagc tcgtgatact cgtatttctg
     4381 gtgaaatgct ggaatctgct ttgattgctg gtctactttc agtaggtatt gaggtttata
     4441 aacttggtgt tttagccact cctggagtgt cttaccttgt tcgtacagaa aaagctagtg
     4501 caggggttat gatttcagca agccacaatc cagcacttga taatggtatt aagttctttg
     4561 gtagtgatgg ttttaaactt gatgatgatc gtgaattaga aattgaagct ttacttgatg
     4621 cgaaggaaga cactttacca cgtccatcag cacaaggttt aggtacttta gtagattatc
     4681 ctgaaggact tcggaaatat gagaagttta tggaatcaac tggtattgat ttagaaggta
     4741 tgaatgttgc gttggataca gcaaatggtg cagcaacagc ctcagctcgg aatatctttt
     4801 tagacttgaa tgctgatata agtgttattg gtgatcaacc agatggtttg aatatcaatg
     4861 atggtgttgg ttcgacacat cctgagcaat tacaaagttt agttagagaa aatggttccg
     4921 acatcggtct agcttttgat ggtgatagtg atcgtttgat cgctgttgat gaaaatggag
     4981 agattgtaga tggtgataaa attatgttta tcattgggaa atatctctct gataaagggc
     5041 agcttgctca gaatacaatc gtaacaacgg ttatgtcaaa tctcggcttt cataaagcgc
     5101 tcgaccgtga aggaattcat aaagcgatta ccgctgtagg agaccgctac gtggttgaag
     5161 aaatgcgtaa atcaggatat aatcttggcg gagagcaatc aggccacgtt attatcatgg
     5221 attacaatac tacaggggat ggacaattaa cagccatcca attgactaag gtcatgaaag
     5281 aaactggtaa gaaattatca gagttagcta gtgaagtgac ggtttatcct caaaaattag
     5341 taaatattcg tgttgaaaat aacatgaaag ataaagcgat ggaagttcct gcaattgcgg
     5401 aaattattgc taaaatggaa gaagagatgg atggcaatgg tcgtattcta gtacgtccta
     5461 gtggtactga gcctcttctt agagttatgg cagaagcgcc aacaaatgaa gcggttgatt
     5521 attatgttga tactatcgca gatgtcgttc gtacagaaat tggtttagat taaaacggcg
     5581 ttattctgct ataaagtcgt caatgttata aaataataaa gagagggaat tggagtggag
     5641 catcaagcat acgcctttct ctctcttttt gatttgagaa gggagtttag agaaaatgac
     5701 taaagaagca gaattattat gggctaaaca caagtattta gtcttaagta aatctcagaa
     5761 aatttatttg gatattagac aaactttgaa atcaccaaat tgtaccgttt tagacgttca
     5821 gtcactcatt gaccaagcag tgcttttaga agaatcacca tcacaagtga ctaatgctta
     5881 tatgcatata tggggctatt ttaaaaataa ggcagagcgt caagaaaaag aagaattttt
     5941 aaccttgctg gaaaaataca ggaaaacagg ttatcaacga agaaaactgt tagcttttct
     6001 aaaacagcta ttagctaaat acccaaattc ttatttacaa aactcttcta ttttcgaaga
     6061 agagtaaaag attgatagtt gaggtatata aatgagatta tggcatcaag atattattga
     6121 gttattgcct aggcagcaac ttttaggaca gcatagagag tgttgtgcat tgagagggaa
     6181 tgggtggggg agaaaacatg agactgttga ttatgttttt cgatattcac cttataggtt
     6241 atttgcatat catcaattag ttatggaaga aatgatggag cgtgggtatc gtgtatctaa
     6301 agaatggcta attgcagaat ataggggaat gaagtgccca agatatgata cacttaatcc
     6361 tgttgacttg gagacaccta tctatcctga gcacaatcaa gactatctac aagaatgtct
     6421 atggaattta aaagcaaaag gaattgacct tcctataaat aagataaaat agagatttat
     6481 ctctattttt attttggaat aaaaagctat attatcaagt tttagaggag tgttttttta
     6541 agggcaaagt ggtaaaatag aaacatgtta aagaaaccaa cctcagcata tgtacacatt
     6601 ccattttgta cccaaatatg ttattattgt gatttttcaa aagtttttat taaaaatcag
     6661 cctgtagacg cttatcttca ggctcttatc cgtgaatttc ggtcttatga tattacagaa
     6721 ttgcgcacct tatatattgg aggagggaca cctacatcta taagcgctgt gcaactggac
     6781 tatttattga cggagttaag tagagattta aacttaaata ctttagagga gttcactatc
     6841 gaagctaatc caggagattt aaccgttgat aaaatagagg tgcttcaaaa gtcagctgtc
     6901 aatagagttt ctctaggagt tcagaccttt aatgataaac atttaaagcg tattggacgc
     6961 agtcacaatg aggtacaaat ttattctaca attgatgctc ttaaaacagc tggttttcaa
     7021 aatatctcta ttgacctaat ttatgcctta cctggacaaa cgatggatga tgttagaagt
     7081 aatgtagcca aagctctctc tctaaatatt cctcatttga gtctatatag tttaatttta
     7141 gaacaccata ctgtatttat gaataaaatg cgtcgtggga aattacattt gccaacagaa
     7201 gatttagaag ccgagatgtt tgaatatatc atttctgaaa tggagagaaa tggttttgag
     7261 cattatgaaa tttctaattt tacaaagccg ggttttgaaa gtcgtcataa tttgatgtac
     7321 tgggataatg tagaatatta tggtgttggt gcaggtgctt ctggttatct tgatggtatt
     7381 cgttatcgta atagagggcc aatacaacat tatctaaagg gtgtttcaga aggtaatgca
     7441 aggttatctg aagaagtatt atcaaaaaat gaaatgatgg aagaggagct tttcctcggt
     7501 cttcgtaaaa aaaagggtgt ttccatcgga aaatttgaac aaaaatttgg aacgtccttt
     7561 gagaaacgtt acggtcagat tgttcaagaa ctccaaagtg atgggctttt gaaagaaaat
     7621 aatggtttta tccagatgac taaaaaaggt ctcttcttag gagatacggt tgccgagaaa
     7681 tttattgtgg agtaaaaaat gggtttatta tatcgagaaa cttacgaagt gcctttttat
     7741 gaatcagaca ctaatcatta tatgaaatta ccgcagttgt tggctttagc gttacaaatt
     7801 tcagccaaac aatctttaaa attaggaata ggcgatgaca tcgtttttaa aagatacgga
     7861 ttggtttggg tcgttacaga ttatattatt gatattgagc gtttacccaa gcatgctgaa
     7921 aagatagtca ttgagactga agccaaagct cataataaat tattgtgtta ccgctatttt
     7981 tatatttatg gtgaagacgg gcaaaaaata ataacgattt catctgcttt tgtcttaatg
     8041 gattttaaaa cacggaagat tcatcctgtt cttgatgata ttacttcaat ttaccaatcc
     8101 caaagaatca aaaaagttat tcgagggccg aaatatcatc ccattggaga tagtaaagtt
     8161 aaacaatacc atgttcgtta ttttgattta gatatgaatg gacatgttaa taatagtaag
     8221 tatctagaat ggatgtatga tgtgttggac ttagattttc taagtagcca cattcctaaa
     8281 aaaattgatt taaaatatat taaggaaatt caatatggaa cggatattaa gtctcattgg
     8341 tatcaagatg gtcttgtgac acgtcatgat attattggtg gcgatgctat tcatgctcaa
     8401 gctaggattg aatggcagga aaaaaaggag gattaaatgg cttataaagg atatcttatt
     8461 gatttagatg gtactattta caaagggaaa agtcgtatcc cagcaggaga gcgttttatc
     8521 gaacgtttgc aagaaaaagg cattccttat atgttagtaa ctaataatac gactcgcaca
     8581 ccagaaagcg ttcaggaaat gcttcgaggc ttcaatgttg aaacaccact tgaaacaatc
     8641 tataccgcaa ctatggctac ggttgactat atgaatgata tgaaccgtgg aaaaacagct
     8701 tacgttattg gtgaagaagg gcttaaaaaa gctattgcag atgcaggtta tgtggaggat
     8761 acaaaaaatc cagcctatgt ggttgttggt ttagattgga atgttactta tgataaattg
     8821 gcaactgcta cgttagcaat tcaaaatggt gctttattta ttggaaccaa tccagatttg
     8881 aatattccta ctgaacgtgg tttattaccc ggtgctggct ccttaaatgc tttattagaa
     8941 gccgcaacgc gtataaaacc agtctttatt ggaaagccaa atgccatcat tatgaataag
     9001 gcacttgaaa ttttaaatat tcctagaaat caagcagtca tggtcggtga taattatttg
     9061 acagatatca tggctggtat taataatgat attgatactt tgttagtaac aacgggtttt
     9121 acaacagttg aagaagtacc agatttacca attcaaccaa gttatgtttt agcaagttta
     9181 gatgaatgga catttaatga aggataagtt attagtagtt ttaacttgga tttggattat
     9241 atcgttagca actctagcta caatttatat tgcttggttg atctatccta tagaaattca
     9301 gtttttaaaa ttagaaaagg ttgtgtattt aaaggctgaa acaatttatt ataattttaa
     9361 taagttgatg atatatctga ctcatccttt tatctctgat ttaaatatgc ctagctttcc
     9421 ttcttcagag gatggtttaa agcattttgc ggatgtaaaa tatcttttta ctctagcgca
     9481 tgggcttttt gttatactaa cttttccagt tatttatttt ttaagacgtg gatggaagca
     9541 aaaaagtatc tttctatatg aaggcttctt caagatagct attatgttgc ctatttttat
     9601 tgtagtatgt gcttttctgt tgggttttga tcagtttttc actctatttc atgaggtgct
     9661 ttttccagga gattctactt ggcaatttaa tcctctaacg gatccagtaa tttggatact
     9721 tcctgaaact tttttcctcc attgtttcat catttttttg ttaatctatg aaacaataac
     9781 tataattctt ttgataatag gtagaaaaca tttaaaatta agaaaatata aaaacaaaat
     9841 gcaagcctta taatagcgct tgcattttgt ttgctatttt tttataatac aaatat
//
LOCUS       DATROQ010000071         9770 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.072, whole genome shotgun sequence.
ACCESSION   DATROQ010000071 DATROQ010000000
VERSION     DATROQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9770
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.072"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..967)
                     /locus_tag="VBC94_001549"
     CDS             complement(<1..967)
                     /locus_tag="VBC94_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6979808.1"
                     /translation="MLFDFLEEKIKLKAYLMTALLDDGEINLDHFKTEYALSEKILQG
                     LISELQILYSDFYLVVKSRTMVLFHYETLSKLETLHTIYRESNTLQFLAYFIAPQNVS
                     FSEFTAKKFISVASAYRIKKNCKLYLESVKLSIQKNKVTGPEYQIRLLIALLQYHFGF
                     IIYPFDATSEVMIRDFIEYSNFKGCDYDLKDLSEEYYYFYILVNLVWKRKEYKVEIPQ
                     TESFNQLKKSILYMELSKAAQKSIAIHLGIELNEGLLDYLYLVFCISDGPLFSNRDDI
                     NINEEFIFASYKISKSKAFKNFCGRLLGREFIDSQHFKDINAYFLR"
     gene            complement(1099..1341)
                     /locus_tag="VBC94_001550"
     CDS             complement(1099..1341)
                     /locus_tag="VBC94_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEO6979809.1"
                     /translation="MDTIENLIIAIVKPLISQPDQLTIKIQDGPEFLEYHLDLDTQDI
                     GRVIGKKGRTITAIRSIVYSVPTQGKKVRLIIDEKE"
     gene            complement(1351..1623)
                     /gene="rpsP"
                     /locus_tag="VBC94_001551"
     CDS             complement(1351..1623)
                     /gene="rpsP"
                     /locus_tag="VBC94_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEO6979810.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQVTIKEERVLEWLSKGAQPSDTVRNLLSKAGVMTKFHDQKFSK"
     gene            complement(1752..2999)
                     /locus_tag="VBC94_001552"
     CDS             complement(1752..2999)
                     /locus_tag="VBC94_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6979811.1"
                     /translation="MENWKFALSSILGHKMRAFLTMLGIIIGVASVVLIMALGKGMKD
                     SVTNEITKSQKNLQIYYKTKEDQKNEDNFGAQGAFMQGSDTNRKEPIIQESWLKKIAK
                     EVDGVSGYYVTNQTNAPVAYLEKKAKTVNITGINRTYLGIKKFKIKSGRQFQEEDYNQ
                     FSRVILLEEKLAQRLFQTNEAALNKVVTVKNKSYLVVGVYSDPEAGSGLYGSNSDGNA
                     ILTNTQLASEFGAKEAENIYFHLNDVSQSNRIGKEIGKRLTDISHAKDGYYDNFDMTS
                     IVKSINTQVGIMTGVIGAIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRRK
                     ILAQFLIESMVLTILGGLIGLLLAYGGTMLIANAQDKITPSVSLNVAIGSLIFSAFIG
                     IIFGLLPANKASKLNPIDALRYE"
     gene            complement(3014..3724)
                     /locus_tag="VBC94_001553"
     CDS             complement(3014..3724)
                     /locus_tag="VBC94_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979812.1"
                     /translation="MTTNRKELIRLHQIVKSYQNGDQKLQVLKNIDLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDSPTSGDYSLNGKRVEELSQTKLAQVRNKEIGFVFQQFFLL
                     SKLTALQNVELPLIYAGVAPKKRKNLAKQFLDKVELRERMNHLPTELSGGQKQRVAIA
                     RALVNSPSIILADEPTGALDTKTGEQIMQFLTELNQEGKTIIMVTHEPEIADYATRKI
                     VIRDGEITADTTDSIRID"
     gene            complement(3727..4971)
                     /locus_tag="VBC94_001554"
     CDS             complement(3727..4971)
                     /locus_tag="VBC94_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO6979813.1"
                     /translation="MSKRQNLGISKKGAIISGLSVALIVAVGGFLWVQSQPNKSAVKT
                     NYKVFNVREGSVSSSTLLTGKAKANQEQYVYFDANKGNRATVTVKVGDKITAGQQLVQ
                     YDTTTAQAAYDTANRQLNKVARQINNLKTTGSFPAMESSDQSSSSSQGQGTQSTSGAT
                     NRLQQNYQSQANASYNQQLQDLNDAYADAQAEVNKAQKALNDTVITSDVSGTVVEVNS
                     DIDPASKTSQVLVHVATEGKLQVQGTMSEYDLANVKKDQAVKIKSKVYPDKEWEGKIS
                     YISNYPEAEANNNDSNNGSSAVNYKYKVDITSPLDALKQGFTVSVEVVNGDKHLIVPT
                     SSVINKDNKHFVWVYNDSNRKISKVEVKIGKADAKTQEILSGLKAGQIVVTNPSKTFK
                     DGQKIDNIESIDLKSNKKSEVK"
     gene            complement(5173..6771)
                     /locus_tag="VBC94_001555"
     CDS             complement(5173..6771)
                     /locus_tag="VBC94_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEO6979814.1"
                     /translation="MLNTKDKQPILLIIFGQKMKEYDDLIRSTVERLSKRNRPVSILI
                     SKELNHVIHHYDAQFENLETLVGYINTHEDSLIYPLCLDKNHELFQEFLAWQSHYSRQ
                     YISDNLSEKSVQAIDNNQDFVQVDCLVMRDSLNNCLYVSDLEYIESNKTTGKSLAIVP
                     SQTLSDAARQTIRDVAFDVCRKANIIGVCYFSFLIDLNSLDYHIISLSSGLSYQSILF
                     ETITTYPVLEIATKLTVGYTFSQLKHSYYPNTSAFLEPQLDYVATVSFSFEKVDYIFF
                     ARNIEQLFLNLLEASSHDHFPFLSDISEEDLMFALIQKKENRLAYLLEAFRRGFDLYD
                     LSSVTKINPFYLDKCLHIVELYENLNKSQYNVDIYKDAKRYGFSDDYIASSWQISLID
                     MLEYRKKHSVAPVLKQVEQSSGVLTGHQIQYFRSYDWHSDYSSSGCQKALIMVDKGYS
                     LVKLNELIKQIKQTHLELLIVTNQPLLIEQLNDTSIIFDTIGIETILTIMGIEEVEEV
                     YLLGESQADMFSKMKNFSISCQSI"
     gene            complement(6827..7897)
                     /locus_tag="VBC94_001556"
     CDS             complement(6827..7897)
                     /locus_tag="VBC94_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEO6979815.1"
                     /translation="MAKKLLILEDGTVFEGLSFGSSLDVTGELVFCTGNTGYQEIITN
                     PSHNGKILVFTSPLIGNYGIHRSYSEAIIPTCLGVVVAEYSRCVSSDTSKMNLDEFLK
                     MKKVPAMSGVDTRYLMQVIKEKGFVKATLAEAGDVLSHLQDQLIATVLPTNNVEQVST
                     KTAYPSPASGRNIVVLDFGLKHSVLRELSKRQCDVTVIPYNTSLEGIKNLYPEGIILS
                     NGPGNPEKLQEVLNTIKELQKSVPMLGIGLGHQLIAMANGAEIMRLPVAKKGPNYPMR
                     DIATGRLETVIQFNHFTVNRLNLPHDLLVTHEGLNDQEIVALRHRSFPVMSVQFYPEA
                     APGPHDVTYFFDEFLEMIGSRA"
     gene            complement(7946..8467)
                     /gene="pyrR"
                     /locus_tag="VBC94_001557"
     CDS             complement(7946..8467)
                     /gene="pyrR"
                     /locus_tag="VBC94_001557"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEO6979816.1"
                     /translation="MKRKEIIDDVTMKRAITRITYEIIERNKNLDNIVLAGIKTRGVF
                     LAKRIQERLKQLENLDIPVGELDTKPFRDDMKVEVDTTTMPVDITDKDIILIDDVLYT
                     GRTIRAAIDNLVSLGRPSRVSLAVLIDRGHRELPIRADYVGKNIPTSQFEEILVEVME
                     HDGYDRVSIIDPS"
     gene            complement(8643..9533)
                     /locus_tag="VBC94_001558"
     CDS             complement(8643..9533)
                     /locus_tag="VBC94_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6979817.1"
                     /translation="MEITIKIAGVRLDKALADNSELSRSQANEEIKKGIVLVNGQVKK
                     AKYTVQEGDRITFDIPKEEVLDYQAENIPLDIIYQDDDVAVVNKPQGMVVHPSAGHSS
                     GTLVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKNDRAHQVLAEELKDKKS
                     LRKYLAIVHGNLPNDRGVIEAPIGRSDKDRKKQAVTAKGKPAITRFHVLERFGDYTLV
                     ELSLETGRTHQIRVHMAYIGHPLAGDPVYGPRKTLGGKGQFLHAQTLGFTHPSNGENL
                     IFSVEVPEIFQTTLDKLRKN"
     gene            complement(9517..>9770)
                     /locus_tag="VBC94_001559"
     CDS             complement(9517..>9770)
                     /locus_tag="VBC94_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEO6979818.1"
                     /translation="CVAIIYLMKHINGSYWLLISLTLIISGGLGNFIDRLRLGYVVDM
                     VHLDFINFAIFNVADSYLTIGIICLMIALWKEESNGNHN"
BASE COUNT         3096 a         1911 c         1456 g         3307 t
ORIGIN      
        1 gtctcaaaaa ataggcatta atatctttaa aatgctgcga gtcaataaat tctctaccca
       61 ataatcgacc acaaaaattc ttaaaggctt tagactttga tatcttataa gaagcaaaaa
      121 taaattcttc gttgatattg atatcatctc gattgctaaa tagaggacca tcactaatac
      181 aaaacactaa atatagataa tcaagtaaac cttcattcaa ctctatacca agatgtatag
      241 caatactctt ttgagctgct ttggataact ccatgtagag aatagatttt tttagttgat
      301 taaaggattc tgtttgagga atttctactt tatattcttt tcttttccaa actaaattca
      361 ccaaaatata aaaatagtaa tattcttcag ataagtcttt aaggtcgtag tcacaccctt
      421 taaaattcga gtattcaata aaatccctta tcattacctc acttgtagca tcaaaaggat
      481 aaataataaa accaaaatga tactgtaaca aagcaatcag cagcctaatc tgatattctg
      541 gacctgtcac tttatttttt tgtattgata acttaacact ctccaaataa agtttacagt
      601 ttttctttat tcggtaggca cttgcgacgg atatgaattt cttagccgtg aattcagaaa
      661 aactgacatt ttgaggagca ataaaatatg ccaaaaactg taacgtatta gactctcgat
      721 aaatggtatg caaagtctct agcttagaaa gtgtttcgta atggaacaat accattgttc
      781 tacttttgac aacaaggtag aaatcagaat aaagtatttg tagttccgaa ataagacctt
      841 gtaaaatctt ttcactcaaa gcatattctg ttttaaagtg atcaaggtta atttctccat
      901 catcaagtaa ggcggtcatc aggtatgctt tcagttttat tttttcttcg agaaaatcaa
      961 atagcaatat cttatcctcc ttaataaaaa atcagaaaat tccctttcac atccattata
     1021 ttctaaaaga ctaaaaaact cacaattttt gtgtagatat aacaaaaaag acaggaagtt
     1081 cctatctttt agaataaatt attctttctc atcaatgatg agtcttactt ttttcccttg
     1141 tgttggtacc gaatagacaa tcgaccttat cgctgtaatt gtacgaccct ttttgccgat
     1201 aacacgaccg atgtcttggg tatctaaatc aagatggtac tctaaaaatt ctgggccatc
     1261 ctgaattttt atggttaact gatctggttg tgaaatcaaa ggtttcacaa tagcaataat
     1321 gagattttca atggtatcca taggctttta ttattttgag aatttttgat cgtggaattt
     1381 agtcataact ccagcttttg aaagaaggtt acgaactgta tctgaaggtt gtgcaccttt
     1441 tgataaccat tcaagaacac gttcttcttt gattgtaact tggttttctg caactaatgg
     1501 gttgtatgtt ccaacagttt caataaaacg tccgtcacgt ggtgcacgtg aatctgcaac
     1561 attgatacgg tagaaaggtt tcttcttaga acccatacga gttaaacgga tttttactgc
     1621 catttttaaa tagtctcttt tctttttctt tatatttggt cgttgatagc ttaactaaca
     1681 actttacctt actaagtata acataaatac aaactctgtc aagaaaaaat cttgacagag
     1741 tacatgtttt tttattcata tcgtaaagca tcaattgggt ttaatttgct agctttatta
     1801 gcaggtaata aaccaaatat aattccaata aatgctgaga aaattaaact gcctatagcc
     1861 acgttaagtg acacagaagg agtaatctta tcttgcgcat tagcaattaa catcgtacct
     1921 ccataggcta gtaataaccc aattaaccca cccaaaatcg ttaagaccat tgattcaatt
     1981 aaaaattgag ctaaaatttt acgtcgagtt gccccaaggg ctttacgaag gccaatttct
     2041 ctcgtgcgct cagttacaga gactaacata atattcatca ctccgatacc accaactagg
     2101 agagaaattg ctgcaatcgc tccaataaca cctgtcataa taccaacttg ggtattgata
     2161 gatttaacta tagatgtcat atcaaaatta tcatagtacc catccttagc atggctaatg
     2221 tctgttaaac gtttgcctat ttctttacca atgcgattgg attgcgacac atcattaaga
     2281 tgaaaatata tgttttctgc ttctttagca ccaaactccg aagctaactg tgtattggtt
     2341 aaaatagcat tcccatcaga attacttcca tataaacctg agccagcttc tggatccgaa
     2401 taaaccccca ctactaagta agatttattt tttacagtca ccactttatt taacgctgct
     2461 tcatttgtct gaaataatct ctgagctaat ttttcttcta gtaaaataac tctcgagaat
     2521 tggttataat cctcctcttg gaattgacga ccacttttaa ttttgaactt tttgatacca
     2581 aggtaagtgc ggttaattcc tgttatatta accgtctttg cctttttttc aaggtaagct
     2641 actggagcat ttgtttgatt ggttacatag tagccactaa caccatctac ttccttagca
     2701 atctttttta accatgattc ttgaataatt ggttccttac ggttggtatc actaccttgc
     2761 atgaaagccc cttgagctcc aaagttatcc tcattttttt ggtcttcttt tgttttataa
     2821 taaatttgaa gattcttctg agactttgta atctcatttg taaccgaatc tttcatgccc
     2881 ttacctaagg ccataattaa cacaactgat gcaaccccaa tgataatccc taacatggtc
     2941 agaaaagcac gcatcttgtg ccctagaatc gatgataggg caaatttcca attttccata
     3001 atcgaatctc cccttaatca attctaatac tatcagtagt atctgctgta atttcgccat
     3061 cacgaataac aattttacgt gtcgcataat cggcgatttc tggttcatga gttaccatga
     3121 taatggtttt accttcttga tttaattccg ttaggaactg cataatttgc tcacccgtct
     3181 ttgtatccaa ggcaccagtc ggctcatctg ctagaataat agatgggcta ttcaccaaag
     3241 cacgcgcgat tgctacacgt tgcttctgtc ccccagataa ttctgttggc agatggttca
     3301 tacgctctct caattcaact ttatctaaaa attgttttgc taaattttta cgctttttag
     3361 gagctacacc cgcataaatt aagggtaatt caacattttg caaagccgtt agtttagata
     3421 acaagaaaaa ttgttgaaag acaaaaccga tttccttatt tcgaacttga gctaattttg
     3481 tttgacttag ttcttcgaca cgtttaccat ttaaactata atctcctgaa gttggggaat
     3541 ccaacaagcc aataatattc attagtgtgg atttccctga gccagatggc cccatgattg
     3601 caagaaattc accttcataa accgtcaaat caatgttctt taagacttga agtttttgat
     3661 ccccattttg ataactttta acaatctgat gcaatctaat cagttctttt cgattagttg
     3721 tcacgattat ttcacctctg atttcttatt agacttaaga tcgattgatt caatattatc
     3781 aattttttgc ccatccttga aagttttgct tggattagta accacgattt gtcctgcttt
     3841 caaacctgat aaaatttctt gtgtcttagc atcagcttta ccaattttga cttcaacttt
     3901 ggaaatttta cgattagaat cattgtatac ccaaacaaag tgtttattat ctttgtttat
     3961 cacagaactt gtagggacaa taaggtgctt atctccatta actacttcaa ctgatacggt
     4021 aaaaccttgt tttaatgcat cgagagggct agtaatatct actttatatt tataatttac
     4081 agcactagag ccgttattag agtcattgtt gtttgcttct gcttctggat aatttgagat
     4141 atatgaaatt ttaccttccc attccttgtc aggatagacc ttagatttta ttttaacagc
     4201 ctgatctttt ttaacattag ccaaatcata ctcactcatc gttccttgta cttggagttt
     4261 accttcagtt gctacatgga caagtacttg actagttttt gaagctggat caatatcact
     4321 attaacttca acaactgtcc ctgatacgtc acttgtaata acagtatcat tcaatgcttt
     4381 ttgtgcttta tttacttctg cctgtgcatc tgcataagca tcattcaaat cttgaagttg
     4441 ttggttgtat gaagcattag cttgactttg ataattttgc tgtagacgat tcgtcgcacc
     4501 actagtcgat tgagtccctt gtccttgtga tgatgatgaa gattgatcac ttgattccat
     4561 agctggaaaa ctccctgttg tctttagatt attaatctga cgcgctactt tatttaattg
     4621 acgattagca gtgtcgtagg ctgcttgtgc agttgttgta tcatattgaa ctaactgctg
     4681 accagctgtg attttatcac ccactttaac tgtaacagtt gctcgattac ctttattagc
     4741 atcaaaatac acatactgtt cttgattagc cttagctttt cctgtcaaaa gagttgagga
     4801 cgaaacactt ccttctctaa cattaaaaac tttgtaatta gtttttactg cactcttatt
     4861 aggttgagat tgtacccata aaaagccacc tactgctaca attagtgcca ctgagagccc
     4921 tgatataatt gctccttttt tactaatccc taaattttgt cgtttactca tgtccatctc
     4981 ctgtttgtat tattaatata gaacaagtgt attattttta aataatattg tcaagaaaat
     5041 tctttaaaaa taatattttt actcattaag tatactattt tgctcataga aagaacttac
     5101 aaaattgaaa gtactttact ttttaccaca aataaaagct gagatctata tctcagcttt
     5161 tattaagaat tgctaaatag actgacaaga gatagaaaaa tttttcattt ttgaaaacat
     5221 gtctgcttga ctttctccca ataaatacac ttcttccacc tcttctatac ccataattgt
     5281 taatatggtc tcaataccta tagtgtcaaa aattatagat gtatcattta attgttctat
     5341 gaggagaggt tgattggtaa caattaataa ttctaaatga gtttgtttga tttgcttaat
     5401 taactcattt aattttacta gggaataacc cttgtctacc attatcaaag ccttttgaca
     5461 accgctagaa ctgtagtctg aatgccaatc atagcttcga aaatactgaa tttgatggcc
     5521 agttagaaca ccagaacttt gttcgacttg cttcagaaca ggtgcgacac tatgtttttt
     5581 tcgatattct aacatgtcta tcaaagatat ttgccaacta ctagcaatat agtcatccga
     5641 aaaaccatac cttttagcat ccttgtaaat atctacatta tattggcttt tattcaggtt
     5701 ttcatacaac tctacaatat gtaaacattt atctaagtaa aaagggttga ttttagtaac
     5761 tgaggataaa tcatacaaat caaagccacg tctaaaagcc tctaaaagat aagctagacg
     5821 attttctttt ttctgaatca aagcaaacat caaatcttcc tcgctaatat cactcagaaa
     5881 tggaaaatga tcatgactgc tagcctcgag taaatttaaa aataattgct ctatattacg
     5941 agcaaaaaag atatagtcaa ctttttcaaa actaaaagat acggtagcta catagtctaa
     6001 ttgcggctct aaaaaggcag aggtatttgg gtaataagaa tgcttcagtt gactaaaagt
     6061 atatcccact gtcaatttag ttgctatctc taaaacagga taggtcgtaa tagtctcaaa
     6121 taaaatgctt tgataagaca aaccagatga taagctaatt atatgataat ccaaactgtt
     6181 caaatctatt aagaaactaa agtaacatac tccaataatg tttgcctttc gacacacatc
     6241 aaatgcaaca tctcgaattg tttgcctagc agcatcggat aaagtttgac ttggtacaat
     6301 cgctagagac ttaccagttg ttttattaga ttcaatatat tctaaatcag agacatataa
     6361 acaattattc aaagagtctc tcattactaa acaatctact tgcacaaagt cttgattatt
     6421 gtctattgcc tgaacacttt tttctgataa attatcactg atatactgcc ttgaataatg
     6481 actctgccac gctaaaaact cttgaaatag ctcatgattt ttatctaagc aaaggggata
     6541 aatcaaacta tcttcatgtg tatttatata ccccacaagc gtctcaagat tttcaaattg
     6601 tgcatcataa tggtgaatca catggtttag ctccttcgag ataagaatac taacaggtct
     6661 attcctttta gataacctct caacagttga tctaattaaa tcgtcatatt ctttcatttt
     6721 ctgtccgaaa ataataagca aaataggctg tttatccttt gtatttagca tatacgcacc
     6781 tccttattaa tcagctcacc gactgacttt aaataatacc ttatgtttaa gcgcgtgatc
     6841 caatcatttc taaaaattca tcaaaaaaat aggtcacatc atgagggcct ggagcagcct
     6901 ctgggtaaaa ttgcacggac atcactggaa atgagcggtg ccttagagca acaatctctt
     6961 gatcattcaa accttcgtgt gttaccagta aatcatgcgg aagatttagc cggttaacgg
     7021 taaaatgatt aaactgaata actgtttcta aacgccccgt agcaatatct ctcatgggat
     7081 aatttggccc cttcttagca actgggagtc tcataatttc tgcaccattt gccatggcaa
     7141 ttaattgatg ccccaaacca atacctaaca taggaacaga tttttgtaat tctttaatag
     7201 tgtttaagac ttcctgaagc ttttctggat ttcccggacc attagataat atgatacctt
     7261 ctgggtatag atttttgata ccttctaaag acgtattgta gggaatgaca gtcacgtcac
     7321 actgacgttt agacaattct ctcaaaacag aatgtttaag gccaaaatca agtacaacaa
     7381 tatttcgacc gctagcaggc gacggataag ctgtttttgt cgaaacttgt tccacattat
     7441 ttgtcggtaa tactgtcgct attaattgat cttgtaaatg tgataaaaca tcacctgctt
     7501 ctgctaaagt agcttttaca aatccttttt ctttaataac ttgcatcaaa taccttgtat
     7561 cgacccccga cattgcagga acctttttca ttttcaaaaa ttcatctaga ttcatcttgc
     7621 tagtatcgct acttacacat ctggaatact ctgcaacaac aactcctaaa caagtgggaa
     7681 taatggcttc agagtaacta cgatgaatac cataatttcc tattagcggt gaggtaaaaa
     7741 ctaaaatttt tccattatgt gaaggattcg ttattatttc ctgatagccg gtattccccg
     7801 tacaaaaaac taattcgcca gttacatcaa gagatgaacc aaaggatagt ccttcaaaaa
     7861 ccgtaccatc ctctagaatt aacaattttt ttgccattgt ggttcttccc cttctataaa
     7921 atcatcgtaa ataatagtcg tctacttaac tcggatcaat aatactcaca cgatcgtaac
     7981 catcatgttc catcacttca actagaattt cttcaaattg acttgttgga atatttttcc
     8041 ccacataatc tgcacgaatg ggtagttcac gatgcccacg gtcaattaat acagctaaac
     8101 tgacacgact cggacgcccc aaactaacaa gattatcaat agcagctcga atagtgcgac
     8161 ctgtatagag aacatcatca attaatataa tatctttatc agtgatgtca actggcatag
     8221 ttgtggtgtc aacttcaact ttcatatcat cacggaaagg tttagtatcc aattctccaa
     8281 cgggaatatc taaattttct aattgcttca aacgttcttg aatgcgtttg gctaaaaaaa
     8341 cacctcttgt tttaatgcct gctaaaacaa tattatctaa atttttgtta cgttctataa
     8401 tttcgtaggt aatacgtgta atagcacgtt tcatggtcac atcatcaata atttcttttc
     8461 ttttcattgc agtatcctct ctaagataaa agagcccctt caaaaggaga ctctctagaa
     8521 tgacaaagcc gctcaggcat tgatttagtc tgaagtttct ccttaactat ctcactggat
     8581 aaggttaaag gaatgatttg cttatattat agcaaatggt aaaagtattt ttcaagaaaa
     8641 acttaatttt ttctcaactt gtctaaagtt gtctgaaaaa tttccggaac ctctactgaa
     8701 aaaatcaaat tctctccgtt actagggtgc gtaaaaccta gtgtttgtgc atgcaaaaac
     8761 tgtcctttac cccctaaagt cttgcgggga ccgtagacag gatcaccggc tagaggatgg
     8821 ccaatataag ccatatgaac ccgaatttgg tgcgttcgac ctgtctccaa tgacaattca
     8881 actaaggtat aatcaccaaa acgttctaaa acatgaaaac gagttattgc tggcttacct
     8941 ttagcagtca cggcttgctt cttacgatct ttatcacttc taccaatcgg agcctcaatc
     9001 acgcctcgat catttggtaa attaccatgc acaattgcta aatatttacg aagcgatttt
     9061 ttatctttca actcttcagc caacacttgg tgagctctat cattttttgc aaccatcaat
     9121 aaaccagaag tatctttatc aatacgatga acgatccctg ggcgaacaac gccattaatt
     9181 gatgataaat ccttaatatg atacatcaag gcgttaacta aggtgcccga actgtggcct
     9241 gcagaagggt gaacaaccat cccttgcggc ttattaacca cagcaacgtc atcatcttga
     9301 tagataatat ctaaagggat attttcagct tgataatcta gtacttcctc tttaggaata
     9361 tcaaaagtaa tccgatctcc ttcttgaaca gtatatttcg ctttttttac ttgtccattt
     9421 actaacacaa tccctttttt aatttcttca ttagcttgac tacgcgacag ttcagaatta
     9481 tcagctaaag ctttatctag cctaactcct gctattttaa ttgtgatttc cattactttc
     9541 ctctttccaa agtgcaatca ttaagcaaat aataccgatt gtcagatatg agtctgctac
     9601 attaaagata gcaaaattaa taaaatctaa atgcaccata tcaacgacat aacctaatct
     9661 taagcgatct ataaagtttc ctaatccacc agagatgatt aaggttagag aaattaataa
     9721 ccaataactt ccattaatat gtttcatcaa ataaataata gcaacacaaa
//
LOCUS       DATROQ010000072         9634 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.073, whole genome shotgun sequence.
ACCESSION   DATROQ010000072 DATROQ010000000
VERSION     DATROQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9634
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.073"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            97..1758
                     /locus_tag="VBC94_001560"
     CDS             97..1758
                     /locus_tag="VBC94_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO6979819.1"
                     /translation="MSDIKIIALGGVRENGKNLYVVEVNDSIFVLDAGLKYPENEQLG
                     VDVVIPNLDYLIENKKRVQGIFLTHGHADAIGALPYIIAEVKAPVFGSPLTIELAKLF
                     VKNSTAVKKFNNFHVIDSETEIEFQDAVISFFKTTHSIPESMGIVIGTKEGNIVYTGD
                     FKFDQAARKYYQTDLARLAEIGRDGVLALLSDSANATSNEQVASEYEVGDEIKSVIED
                     AEGRVIVAAVASNLIRIQQVFDAAAENGRRVVLTGFDIENIVRTAIRMKRIHIADENM
                     IIKPKDMTRYEDNELLILETGRMGEPINGLQKMAIGRHRYVQIKDGDLVFIVTTPSIA
                     KEAVVARVENLIYKAGGSVKLITQNLRVSGHANGRELQLLMNLLKPKYLFPIQGEYRD
                     LSAHAGLAQEVGMSADDIYIVKRGDIMVLEKDGFFHSGSVPAGDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVVITVSKKEKKIISKARVNTRGFVYVKKSRDILRESAELVNTT
                     VEDYLSKDTFDWGELKGKVRDEVSKFLFDQTKRRPAILPVVMEVR"
     gene            1815..2603
                     /locus_tag="VBC94_001561"
     CDS             1815..2603
                     /locus_tag="VBC94_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEO6979820.1"
                     /translation="MAFFNIEYHSKVLGTERQVNVIYPDAFEMSDDKIDDCDIPVLYL
                     LHGMGGNENSWQKRTNIERLLRHTNLIVVMPSTDLAWYTNTKYGLDYFDAIAIELPKV
                     LKRFFPNMSDKREKNFIAGLSMGGYGAYKIALLTNRFSHAASLSGALSFDFDLLFNNG
                     NDNTNYWSGIFGDLNNTDNIERHSLRRYVESFDMKTKFYAWCGYEDFLFEANEVAIDE
                     LRQLGLTIDYSNDHGKHEWYYWNQQLEKVLEWLPVDYVKEERLS"
     gene            2693..3403
                     /locus_tag="VBC94_001562"
     CDS             2693..3403
                     /locus_tag="VBC94_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEO6979821.1"
                     /translation="MRTLFRMIFAIPKFIFRLIWNIIWGIFKTVLVIAIILFGLYYYA
                     NHSQSEFANQLSDIIQTGKTFLNFADTNQLKNSFANLATDNVHHSENTRWDKNQATIY
                     IATRDSELASAYKQAISNWNATGAFVLQTTDNPNADIIAKDYSDAKTQAAGVAETERN
                     ALTNRISKVTVKLNTFYLKNKQFGYDHTRIVNTAEHELGHALGLGHNDQQHSVMQSKG
                     SHYGIQEVDIQTLKNLYA"
     gene            3641..4312
                     /gene="rpiA"
                     /locus_tag="VBC94_001563"
     CDS             3641..4312
                     /gene="rpiA"
                     /locus_tag="VBC94_001563"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEO6979822.1"
                     /translation="MDELKKLAGVTAAKYVKNGMIVGLGTGSTAYFFVEEIGRRVKEE
                     GLQVIGVTTSNRTTEQARGLGIPLKSADDIDVIDVTVDGADEVDPDFNGIKGGGGALL
                     MEKIVATPTKEYIWVVDESKLVETLGAFKLPVEVVRYGSERLFRVFKSKGYCPSFRET
                     EGDRFITDMGNYIIDLDLKKIEDPKQLANELDHTVGVVEHGLFNGMVNKVIVASKNGL
                     DILEK"
     gene            4369..5580
                     /locus_tag="VBC94_001564"
     CDS             4369..5580
                     /locus_tag="VBC94_001564"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO6979823.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHDMGHNKSNSHGIDTL
                     IKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPEIVSAMRDKDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADISATVADNFGVDTA
                     MIGESFLQDLV"
     gene            5631..6038
                     /gene="arsC"
                     /locus_tag="VBC94_001565"
     CDS             5631..6038
                     /gene="arsC"
                     /locus_tag="VBC94_001565"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416958.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEO6979824.1"
                     /translation="MEKVRIYHNPNCGTSRNVLAIIRHCGIEPEIIYYLKTPPSKMAL
                     VELLLEMKLSVRELLRTDVPAYEKFNLESSSVTDEEMIDAMIQDPVLINRPIVVTSKG
                     AKLCRPCEEILTILPVKMEKDFVKEDGQIIQSL"
     gene            6077..6886
                     /locus_tag="VBC94_001566"
     CDS             6077..6886
                     /locus_tag="VBC94_001566"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO6979825.1"
                     /translation="MTLLEKINETRDFLQAKGVTAPEFGLILGSGLGELAEEIENPIV
                     VDYADIPNWGQSTVVGHAGKLVYGDLSGRKVLALQGRFHFYEGNTMEVVTFPVRIMRA
                     LACHSVLVTNAAGGIGYGPGTLMLIKDHINMIGTNPLIGENLEEFGPRFPDMSDAYTA
                     TYRQKAHQIAEKQNIKLEEGVYLGVSGPTYETPAEIRAFQTMGAQAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQRIKEDFKGLVKSLVAEL"
     gene            6888..8144
                     /locus_tag="VBC94_001567"
     CDS             6888..8144
                     /locus_tag="VBC94_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEO6979826.1"
                     /translation="MVSRLYYAVKFMIAVLFMTVMAGVGAILMHYVLMFTEWLAFGDS
                     RENTLSLLNSVTPIKRVLSLTLVSFLASLSWYYLQIKPKQITSIKQQVVFKDFSVKKS
                     PYWLHIGHAFLQLIYVGAGGPIGKEGAPREFGAINAGKISDLLALKVLDKRLLIVSGA
                     AAGLSAVYQVPLASVFFAFETLALGISLKNIVTLLVSTFGAASIAQLVISTAPLYHIS
                     KMSLNSQSLAFMFLIVLCVTPIAISFRYLNQKVTERRIKNIKILLSLPVVSLIVSVLS
                     IIYPQILGNGNALVQEVFKGTTVSLIAILVVLKMIATLSTLYAGAYGGILTPSFSIGA
                     CLGFLLASISIPLLPHLSIVTSMLVGAAIFLAITMRAPLTAVGLVISFTGQSVITIVP
                     LTIAVLFATAYDYFIRKMRSLYVNPY"
     gene            8128..8838
                     /gene="deoD"
                     /locus_tag="VBC94_001568"
     CDS             8128..8838
                     /gene="deoD"
                     /locus_tag="VBC94_001568"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO6979827.1"
                     /translation="MSIHIEAKQGEIADKILLPGDPLRAKFIAENFLEDAVCFNTVRN
                     MFGYTGTYKGHRVSIMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAINPDIHVRE
                     LVLAQAAATNSNIIRNDWPEFDFPQIADFKLLDKAYHIAKEMDITTHVGSVLSSDVFY
                     SNQPDRNMALGKLGVHAIEMEAAALYYLAAQHNVNALAMMTISDNLNNPEEDTSAEER
                     QTTFTDMMKVGLETLISE"
     gene            8847..9614
                     /locus_tag="VBC94_001569"
     CDS             8847..9614
                     /locus_tag="VBC94_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEO6979828.1"
                     /translation="MTDFSTALKVLVEQYSYHNAFLLLRKHGPLNSDLLFLLEMMKER
                     RELNIDFLFAHQEQVVILQEKYNIKLLHNSYDLELLANYIMDLEAKVKNGLIIDFVRS
                     VSPILYRLFMILLAQEVPHLHDYIYNARDDHYDTWKFKELKESNHPVLLAFSERWHDS
                     RLTSKSLAECLQLTDLDEEVKSTIIQLRQFEKSVRNPLAHLIKPFDEQELYRTTQFSS
                     QAFLDQIIFLAKVIGVEYDTVNFHYDTVNKLIIKILE"
BASE COUNT         3057 a         1544 c         1975 g         3058 t
ORIGIN      
        1 aagttaccga taaatcaatt actaatgata ataattggac aatttcttgt ctgctttata
       61 aaaaagaatt aacaaaattt aaggagaaaa ctcattatga gcgacataaa aatcattgct
      121 cttggaggtg ttcgtgaaaa cggaaaaaac ctatatgtag tagaagtcaa tgattctatc
      181 tttgttttgg atgctggact caaatatcct gaaaatgaac aactgggtgt tgatgttgtt
      241 attccaaatc ttgattactt aattgaaaat aaaaaacgtg ttcaggggat tttcttaacc
      301 catggacatg cggatgctat tggtgcattg ccttatatta tcgccgaagt caaagcacct
      361 gtgtttggtt cacctctcac aattgaatta gctaagttat tcgttaaaaa tagtacagca
      421 gtcaagaaat tcaataattt ccatgtgatt gatagtgaaa cggaaattga atttcaagat
      481 gcggtgattt cgttctttaa aacgactcat tcaattccag agtcaatggg aattgttatt
      541 gggacaaaag agggaaatat cgtttacaca ggtgacttta aatttgatca agctgcaagg
      601 aaatattacc aaacagatct ggcgcgttta gctgaaattg gtcgtgatgg tgttttagca
      661 cttttatcag attctgcaaa tgctacaagc aatgagcaag ttgctagcga atatgaggtc
      721 ggtgatgaaa ttaagagcgt tattgaagac gcagaaggta gagtcatcgt agcggcagtt
      781 gcaagtaatc tcattcgtat tcaacaagtc tttgatgcag cggcagaaaa cggtcgtcga
      841 gtagttttaa cgggttttga tattgaaaat attgttcgta cggccattcg tatgaaacgc
      901 attcatattg ctgatgaaaa tatgattatc aaacctaaag atatgacacg ttacgaagat
      961 aacgaattat taatccttga aactggacgt atgggagagc cgataaatgg tctccaaaaa
     1021 atggcgatcg gacgtcatcg ttatgttcaa attaaagatg gtgatttagt atttatcgta
     1081 acaacaccaa gtattgcaaa agaggcagtt gtagcgcgtg ttgaaaatct gatttataaa
     1141 gctggtggct cagttaaatt gataacacag aatttgcgcg tatcaggtca tgcaaatggt
     1201 cgcgaattac aattattaat gaatctattg aaacctaaat accttttccc aattcaagga
     1261 gagtataggg atttgtcagc acatgcgggc ttagctcaag aggtaggaat gtcagcagat
     1321 gatatttata ttgtgaagcg tggtgacatc atggttcttg aaaaagatgg cttcttccat
     1381 agtggatctg ttcctgctgg agatgttatg attgatggaa atgctattgg tgacgttggt
     1441 aatattgtac ttcgtgaccg caaggtatta tctgaagatg gaatttttat tgttgttata
     1501 actgtcagca aaaaagaaaa gaaaattatt tctaaagctc gtgttaatac gcgtggtttt
     1561 gtgtatgtga aaaaaagcag agatattttg cgtgagagcg cagaattagt taatacaacg
     1621 gtagaagatt acttaagtaa agatacattt gactggggtg aacttaaagg gaaagtgcgt
     1681 gatgaggtca gcaagttctt atttgaccaa acgaaacgtc gcccagcaat ccttcctgtg
     1741 gttatggaag ttcgttgata gtgttaagag gttaccaaat tttggtaacc tttcttgcta
     1801 gaatggaggt ttttatggca ttttttaata ttgaatatca ctctaaggta ctaggaacgg
     1861 aacgtcaagt caatgttatt tacccagatg cttttgagat gtctgatgat aaaattgatg
     1921 attgtgacat tcctgttttg tatcttctcc atggaatggg gggaaatgag aattcttggc
     1981 aaaaacgcac aaatatagag cgtcttttac gtcatactaa tcttatcgtc gtgatgccat
     2041 caacagactt agcatggtat accaacacaa agtatgggtt ggattatttt gatgcaatag
     2101 ctattgagtt accaaaagtt ttgaaacgtt tctttcctaa tatgtctgat aagagagaga
     2161 aaaatttcat tgccggatta tctatggggg gatatggagc atataaaata gctctactga
     2221 ctaatcgttt ttctcatgct gcctctttgt caggtgcact ttcttttgac tttgatttat
     2281 tattcaataa tggcaacgat aacactaact attggtcggg aatttttggt gatttgaata
     2341 acactgataa catagagcgt cattcactaa ggcgttacgt tgagtctttt gatatgaaga
     2401 ctaaatttta tgcatggtgt ggttatgaag attttctttt tgaagctaat gaagtcgcaa
     2461 ttgatgaact tcgtcaatta ggtttaacta ttgactattc caatgatcat ggtaaacatg
     2521 aatggtatta ctggaatcaa caattagaaa aagtcttaga atggctccct gttgattatg
     2581 tcaaagaaga gcgcctttct tagtattagc taaatcgttg aaatccaagt aaagtcttgt
     2641 tatactaaat cattaagttt tgggaaatta gacacagaag gtgagataaa aaatgagaac
     2701 actatttaga atgatatttg ctattccaaa gtttatcttt agattgattt ggaatatcat
     2761 ttggggaata ttcaagacag ttcttgttat tgcgattatt ttatttggct tgtattacta
     2821 tgcgaatcac agtcaatcag aatttgctaa tcaacttagt gacattattc agacaggaaa
     2881 aacattttta aattttgctg acactaatca gctcaaaaat tcctttgcta atctagctac
     2941 ggataatgtt catcattcag aaaatactag atgggataaa aatcaggcaa ctatctatat
     3001 agcaactaga gattctgagt tggcctctgc ttacaagcaa gcaatctcca attggaatgc
     3061 aacaggtgct tttgtgttac aaacaacaga taatcctaat gctgatatta ttgctaagga
     3121 ttactcagat gctaaaacac aagctgcagg tgtagctgaa accgagagaa atgctttaac
     3181 taatcgtatt tcaaaggtaa cagttaaatt aaacacattt taccttaaga ataaacagtt
     3241 tggttatgac catactagga ttgttaatac ggccgaacat gaattaggac atgctttagg
     3301 tctaggacat aatgatcaac aacattccgt tatgcagtca aaaggttcgc attatggcat
     3361 tcaagaagtt gatattcaaa cgctaaaaaa cttatatgca taaaaagatg agctaataaa
     3421 ctcatctttt ttgtttgaac acttatttta ggtgatataa gtgcgttagc aatagattat
     3481 taaaaataaa gaatttgact atcactacta actaaaatgc ataaagaaca aagaaaatgt
     3541 tttcaaaaat cacttaaaaa atggtataat gtgtcttgag atagtgtttt gaatagactt
     3601 atcaaaaata ttagataaca ttaacttaaa aggggaaaat atggacgaac taaaaaaact
     3661 tgctggagtg accgctgcaa aatacgttaa aaacggtatg attgtgggtt tagggacagg
     3721 ttctacagca tatttttttg ttgaagaaat tggacgccgt gtcaaagaag agggattgca
     3781 agtcataggc gttacaacgt caaaccgtac gactgagcag gcaagaggat taggtattcc
     3841 gttgaaatca gcagatgata ttgatgtcat tgatgtcact gtggatggag cagatgaagt
     3901 agaccctgat tttaatggta tcaagggggg cggtggtgcg ctgctcatgg aaaaaatagt
     3961 agctactcca actaaagaat atatctgggt tgtcgatgag agcaaacttg ttgaaacgtt
     4021 aggtgcattt aaattgcctg ttgaggttgt tagatatggt tctgagcgtc tttttcgggt
     4081 ttttaaatca aaaggttatt gtccttcttt ccgtgaaaca gaaggtgacc gctttatcac
     4141 tgatatgggg aactacatca tcgacctaga tttgaaaaaa atagaggatc caaagcagtt
     4201 agcgaatgaa cttgaccata cagttggtgt tgttgaacat ggtttattta atggtatggt
     4261 taataaagtt atagtagcaa gcaaaaatgg tcttgacata cttgaaaaat aattatttaa
     4321 ataatcggtt ttaggagcta accgtaacct ccttagaaag aagatataat gtcacaattt
     4381 gatcgcattc acttagttgt cttagattca gtgggtattg gagcggcacc agatgccaat
     4441 gattttgtca atgcaggagt tcctgatggt gcttcagaca cgcttggcca catttcaaaa
     4501 acagttggac ttgcggtgcc aaacatggct aaaatcggac ttggtaatat tcctcgtccc
     4561 caagcgctta agaccgtccc tgcagaagaa aatccaagtg gctacgcgac gaagttacaa
     4621 gaagtatcac ttggtaagga tacaatgaca gggcattggg agatcatggg cttaaatatt
     4681 acagagccat ttgatacttt ctggaatggt tttccagaag atattatcac aaagattgag
     4741 gatttttcag gacgtaaagt tattcgtgaa gcgaataaac cttattcagg gacagctgtt
     4801 attgatgact tcggaccacg ccaaatggaa actggtgagc tcatcatcta tacctcagct
     4861 gatccagtcc tacaaattgc agctcatgag gatatcatcc ctctagaaga actctaccgt
     4921 atttgtgaat atgctcgtag tatcacaatg gagcgtccag ctctccttgg tcgtattatc
     4981 gctcgtccat atgtgggtga accaggcaac tttacgcgta cagccaaccg tcacgactat
     5041 gcagtatcac catttgaaga taccgtatta aataaactag atcaagctgg catcgatact
     5101 tatgctgttg gtaaaatcaa tgatattttc aatggttcag gcattaatca tgatatggga
     5161 cataacaaat caaatagtca tggtattgat actttgataa aaacgatggg gttatcagag
     5221 tttgaaaaag gcttctcctt tactaatttg gttgactttg atgctctcta tggtcaccgt
     5281 cgtgatcctc atggttatcg tgattgtctt catgagtttg atgagcgtct tccagagatc
     5341 gtttcagcga tgcgtgacaa agacttactt ttgatcactg ccgatcatgg taatgaccca
     5401 acttatgcag gtacagacca tacaagagaa tacattcctc tcttagccta tagcccatct
     5461 ttcacaggaa acggccttat cccagtaggg catttcgcag atatttcagc aacagttgcg
     5521 gataactttg gtgttgatac tgcgatgatt ggagagagct tcttgcagga cttggtataa
     5581 atttttattt ccttaagaca cttgtagcat taaaaattga gaggaagatg atggaaaaag
     5641 ttaggattta ccataaccca aattgtggga catcaaggaa cgtacttgcc attattcgtc
     5701 attgtggtat tgaaccagaa attatttatt atctgaaaac accacctagc aagatggcac
     5761 tagttgaact gttgttagaa atgaaacttt ctgtgagaga acttctaaga acagatgttc
     5821 ctgcttatga gaaattcaat ttggaatctt ctagcgttac agatgaagaa atgattgatg
     5881 cgatgataca agatcctgtt cttatcaatc gtcccatagt tgttacgagt aaaggtgcaa
     5941 aactctgccg accctgtgaa gaaattttaa ccattttacc tgtaaagatg gaaaaagatt
     6001 ttgttaaaga agatgggcag attatccaaa gtttatagca tattgtatag tgaataatag
     6061 tttaggaggt aagtaaatga cattattaga aaaaattaat gagactagag actttttgca
     6121 agcaaaaggc gtcacagcac cagaatttgg tcttatttta ggctctggtt taggagaatt
     6181 agctgaagaa atcgaaaatc ccattgttgt ggattatgca gacatcccaa attggggaca
     6241 gtcaacagta gttggtcatg ctggaaaatt agtgtatggg gatttatcag gccgtaaggt
     6301 attagcgctt caaggtcgtt ttcactttta tgaaggaaat acaatggaag tcgttacttt
     6361 cccagtacgt atcatgagag cattggcttg ccacagtgtg cttgtgacta atgcagcggg
     6421 tgggattgga tacggaccag gaactttaat gctgatcaaa gaccacatca atatgattgg
     6481 gactaaccct ctcataggtg agaaccttga agaatttgga ccacgtttcc cagacatgtc
     6541 ggatgcttat acagcaacat atcgacaaaa agctcaccaa attgctgaaa aacagaatat
     6601 caaactcgaa gaaggtgtgt acttgggtgt atcaggaccc acttatgaaa cacctgcaga
     6661 aattcgtgca ttccaaacaa tgggcgcaca agcggtaggt atgtccacgg ttccagaggt
     6721 gatcgttgca gctcactcag ggcttaaagt gttaggaatt tcagcaatta ctaactttgc
     6781 cgctggcttc caatcagagc tcaatcatga ggaggtcgtt gaagttactc agcgtattaa
     6841 agaagatttc aagggattag ttaaatcatt agttgctgaa ctttaaaatg gtttctcgat
     6901 tatactatgc tgtcaagttt atgatagccg ttctatttat gacagttatg gcaggagttg
     6961 gagctatctt aatgcactat gtattaatgt ttacagagtg gttagcattt ggtgatagta
     7021 gagaaaatac tctgtctttg ttaaatagtg tcacaccaat taaaagagtg ttatcactta
     7081 ctttagtctc ctttttagca tcccttagtt ggtattactt acaaatcaag ccaaagcaaa
     7141 taactagtat taaacaacag gttgttttta aggatttttc tgtaaaaaaa tcaccttact
     7201 ggctacatat aggtcacgca tttttgcaat tgatttatgt aggagctggt ggtcctattg
     7261 gaaaagaagg agcgccacga gagtttggag ccataaatgc tgggaaaata tcggatttat
     7321 tagcacttaa agtgctggat aaacgtttat taatcgttag tggtgcagct gcaggtttgt
     7381 cagcagttta tcaagtaccc ttagctagtg tattctttgc ttttgaaact ttagctttag
     7441 gaatttcact gaaaaatatt gtgacattgc tagtctctac ttttggtgca gctagtattg
     7501 ctcaattagt tatttcgaca gcccctcttt atcatatttc aaagatgagt ctgaattcac
     7561 agtcattagc ttttatgttc ttgatcgttt tatgtgtaac tccgattgcg attagcttta
     7621 gatatttaaa tcaaaaagtg acagagagac gtattaaaaa tataaaaata ttgttaagtt
     7681 tgccagtagt gtcattgata gtatctgttt tatcaataat ctatccccag attttaggaa
     7741 atggtaatgc cctagttcaa gaagtcttta aagggaccac agttagctta atcgctattt
     7801 tagttgttct caagatgata gcaacattat ccactcttta cgctggtgct tatggcggta
     7861 tactgacacc atctttttcc attggtgctt gtttaggttt tttgttagct agtatttcta
     7921 ttcctttact tccccaccta tcgatagtaa ccagtatgtt ggttggtgca gctatttttt
     7981 tagctattac aatgagagcg ccactgacag ctgtcggttt agtcatatct tttactggtc
     8041 aatctgttat aactattgtt cccctaacta tagcagtact ttttgctact gcctatgatt
     8101 attttataag aaaaatgagg agtttatatg tcaatccata ttgaagcaaa acaaggtgaa
     8161 attgcagata aaattttact tccaggagat ccactccgtg cgaaatttat tgccgagaat
     8221 tttttagaag atgcggtgtg ttttaataca gttcgcaata tgtttggtta tacaggtacc
     8281 tataaaggac atcgtgtttc tattatggga actggtatgg gtatgccttc aatttcaatt
     8341 tatgctcgcg aactaattgt tgattatggg gttaaaacat tgattcgcgt aggaacagca
     8401 ggtgctatta atcctgatat ccatgttcgt gaacttgttt tggcacaagc agcagcaaca
     8461 aattctaata ttatccgtaa tgattggcct gaatttgatt ttccgcaaat tgctgatttt
     8521 aaacttttgg ataaagctta tcacatcgca aaggaaatgg atattacaac tcatgtaggc
     8581 agtgtattat cttcagacgt gttttattca aaccaaccag atcgtaatat ggctttgggt
     8641 aaactaggtg ttcatgctat tgagatggaa gcagcagctc tctactatct tgcagctcaa
     8701 cacaatgtta atgctcttgc tatgatgacg atttcagaca acttgaataa tccagaagaa
     8761 gatacaagtg cagaagagcg tcaaacaaca tttactgata tgatgaaagt tgggctcgaa
     8821 acacttattt ctgaatgagg atagaaatga cagatttttc aactgctctt aaagtattag
     8881 tggaacagta ttcttatcat aatgctttct tattattacg aaaacatggt cctttaaact
     8941 cagatttatt atttttatta gaaatgatga aagagcgtcg agagttaaac attgattttc
     9001 tttttgctca tcaagaacaa gttgttatat tacaagaaaa atacaatata aagcttttgc
     9061 ataactcgta tgatttagag ttgctggcaa attacatcat ggatttagaa gcaaaagtaa
     9121 aaaatggatt aatcatagat tttgtccgta gtgttagtcc tattttatat cgtctattta
     9181 tgattttact tgcacaagaa gtacctcact tgcatgatta catctataat gcaagagatg
     9241 atcactacga tacttggaag tttaaagaat taaaggagtc aaaccatcca gtccttttgg
     9301 cattctctga aaggtggcac gatagtcgct tgacttctaa aagccttgca gaatgtttac
     9361 aattaaccga ccttgatgaa gaagtgaaat cgaccatcat tcaattaaga cagttcgaaa
     9421 aatcagtcag aaatcctttg gctcacctga ttaaaccttt tgatgagcaa gaactatatc
     9481 gtacaactca attttcttct caagcatttt tagaccagat tatcttcttg gcaaaggtaa
     9541 ttggtgttga gtatgatact gttaattttc actacgatac ggttaacaag cttattataa
     9601 agatacttga gtaatttaaa aacagttctg agac
//
LOCUS       DATROQ010000073         9101 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.074, whole genome shotgun sequence.
ACCESSION   DATROQ010000073 DATROQ010000000
VERSION     DATROQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9101
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.074"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..443)
                     /locus_tag="VBC94_001570"
     CDS             complement(<1..443)
                     /locus_tag="VBC94_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome assembly protein"
                     /protein_id="HEO6979829.1"
                     /translation="MLHSKKIHSLSLIAVLSLATYTSLQPNHVAAEQSQKTSTVHMSQ
                     KTIEHKLKVADKEAAPLYAKIDHIQRHIEVKKAKDLKVIELYINKDINQLEKQNKRLL
                     TKFYTSIDNQTWDSTSEVKKLIDKTTLSTNEKDRLKLYFEQRAYL"
     gene            complement(536..1924)
                     /locus_tag="VBC94_001571"
     CDS             complement(536..1924)
                     /locus_tag="VBC94_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6979830.1"
                     /translation="MKKKSLISNLILINSITLFLGFYLIYFSVNYVIKDYVHQMTRNS
                     MQANFTILDSIYNNKKIPDENMVNQDSIFVWSYYAIFDQHYRMKYGTDNHKKESHDII
                     RYLTQKRLWQISKEKDGMFITIKKKTYYVMTKDYSGILVDGSIKKAPKAQSQLFHVIN
                     FSDITYTQHLITKINHFLIVILVLTYIPMLFIMRKTFTGIRESIQSVQTYISSLWKNQ
                     GNHQSSQKEIVFSDFDPLLLESQEMANRIYQAEESQRNFFQNASHELRTPLMSIQGYT
                     EGVQEGIIDAELAHSVILQESKKMKQLVDDIILLSKLDSNLSDQKDKFSLNELLNSII
                     AYFKPLANKQKISITYRPDKHEKLLKGNEELIQRAINNILSNALRYAVSHIEISYTNQ
                     KLTISNDGPAISKEDLPYIFDRFYKGHGGQTGIGLAMTKEIIKQHHGNIIAESDSTST
                     TFTIYFNCHKIA"
     gene            complement(1921..2606)
                     /locus_tag="VBC94_001572"
                     /pseudo
     CDS             complement(1921..2606)
                     /locus_tag="VBC94_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161341.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(2839..7554)
                     /locus_tag="VBC94_001573"
     CDS             complement(2839..7554)
                     /locus_tag="VBC94_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6979831.1"
                     /translation="MNTKQRFSIRKYKLGAVSVLLGTLFFLGGITNVAADSVINKPSD
                     IAVEQQVKDSPTSIANETPTNNTSSALASTAQDNLVTKANNNSPTETQPVTEPHSQAT
                     ETFSPAANQPVESTQEVSKTPLTKQNLAVKPTPAISKETPQNIDSNKIITVPKVWNTG
                     YKGEGTVVAIIDSGLDTNHDALQLNDSTKAKYQNEQQMNAAKAKAGINYGKWYNNKVI
                     FGHNYVDVNTELKEVKSTSHGMHVTSIATANPSKKDTNELIYGVAPEAQVMFMRVFSD
                     EKRGTGPALYVKAIEDAVKLGADSINLSLGGANGSLVNADDRLIKALEMARLAGVSVV
                     IAAGNDGTFGSGASKPSALYPDYGLVGSPSTAREAISVASYNNTTLVNKVFNIIGLEN
                     NKNLNNGLAAYADPKVSDKTFEVGKQYDYVFVGKGNDNDYKDKTLNGKIALIERGDIT
                     FTKKVVNAINHGAVGAIIFNNKAGEANLTMSLDPEASAIPAIFTQKEFGDVLAKNNYK
                     IVFNNIKNKQANPNAGVLSDFSSWGLTADGQLKPDLSAPGGSIYAAINDNEYDMMSGT
                     SMASPHVAGATALVKQYLLKEHPELKKGDIERTVKYLLMSTAKAHLNKDTGAYTSPRQ
                     QGAGIIDVAAAVQTGLYLTGGENNYGSVTLGNIKDKISFDVTVHNINKVAKDLHYTTY
                     LNTDQVKDGFVTLAPQQLGTFTGKTIRIEPGQTKTITIDIDVSKYHDMLKKVMPNGYF
                     LEGYVRFTDPVDGGEVLSIPYVGFKGEFQNLEVLEKSIYKLVANKEKGFYFQPKQTNE
                     VPGSEDYTALMTTSSEPIYSTDGTSPIQLKALGSYKSIDGKWILQLEQKGQPHLAISP
                     NDDQNQDAVALKGVFLRNFNNLRAKVYRADDVNLQKPLWVSAPQAGDKNYYSGNTENP
                     KSTFLYDTEWKGTTTDGIPLEDGKYKYVLTYYSDVPGSKPQQMVFDITLDRQAPTLTT
                     ATYDKDRRIFKARPAVEHGESGIFREQVFYLKKDKDGHYNSVLRQKGEDGILVEDNKV
                     FIKQEKDGSFILPKEVNDFSHVYYTVEDYAGNLVSAKLEDLINIGNKNGLVNVKVFSP
                     ELNSNVDIDFSYSVKDDKGNVIKKQHHGKDLNLLKLPFGTYTFDLFLYDEERANLISP
                     QSVTVTISEKDSLKDVLFKVNLLKKAALLVEFDKLLPKGATVQLVTKTNTVVDLPKAT
                     YSPTDYGKNIPVGDYRLNVTLPSGYSTLENLDDLLVSVKEGQVNLTKLTLINKAPLIN
                     ALAEQTDIISQPVFYNAGTHLKNNYLANLEKAQTLIKNRVEQTSIDNAIAALRESRQA
                     LNGKETDTSLLAKAILAETEIKGNYQFVNASPLSQSTYINQVQLAKNLLQKPNVTQSE
                     VDKALENLDIAKNQLNGHETDYSGLHHMIIKANVLKQTSSKYQNASQFAKENYNNLIK
                     KAELLLSNRQATQAQVEELLNQIKATEQELDGRDRVSSAENYSQSLNDNDSLNTTPIN
                     PPNQPQALIFKKGMTKESEVAQKRVLRVTSQTDNQKIKTNKLPKTGESTPKITYTILL
                     FSLSMLGLATIKLKSIKRE"
     gene            7877..8569
                     /locus_tag="VBC94_001574"
     CDS             7877..8569
                     /locus_tag="VBC94_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEO6979832.1"
                     /translation="MEKGFKEGVKDALPTALGYISIGLAFGIVASASDLSAIEVGLMS
                     ALVYGGSAQFAMCALLLAKADLMTITMTVFLVNLRNMLMSLHATTIFKSAHLMNQLAI
                     GTLITDESYGVLLGEALHHKVVSPSWMHGNNVMSYLTWVISTIIGTLLGSTIPNPEMF
                     GLDFALVAMFIGLFVFQLFGMLSDGKRLVVYVLASVGLSYFLLSTFLSGALSVLLATV
                     VGCSVGVVLDDK"
     gene            8559..8882
                     /locus_tag="VBC94_001575"
     CDS             8559..8882
                     /locus_tag="VBC94_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEO6979833.1"
                     /translation="MINSTVFLAILLGFVVSWVPRVAPFIFVKYKGLPNSLVKFLKFL
                     PLSILFALTFSSFFSVKLGQLPTLKWLDLLAGIPTLVVTIRYRNLFYTVLTGIVTMAL
                     LRLLF"
BASE COUNT         2610 a         1477 c         1663 g         3351 t
ORIGIN      
        1 tcaaggtaag cacgttgttc aaaatataat tttaatctat ctttttcgtt agtggatagg
       61 gttgtcttat caatcaattt tttgacttca cttgtgctat cccatgtttg attatcaata
      121 gaagtataga atttagttag tagacgttta ttttgcttct ctagttggtt gatatctttg
      181 ttaatataca attcaataac ttttaaatct tttgcttttt tgacttcaat atgtcgttgg
      241 atatggtcga ttttagcgta gagaggagca gcttctttat ctgcaacttt taacttatgt
      301 tcaatagttt tttgactcat atgaacagtt gatgtttttt gtgattgttc agccgctaca
      361 tgatttggtt gtaaactcgt atatgttgct aaagagagaa cggcaataag cgataaggaa
      421 tgtatttttt tagaatgtaa catataaaaa ctcactttct tttagaataa ctttttgatc
      481 tataggtatt ataggttaaa aaagcaataa cttctttgaa tttttgtagg aaaagttagg
      541 caattttgtg gcaattgaaa taaatcgtaa aagttgttga ggtcgaatca ctctcagcga
      601 tgatattacc atgatgttgc ttgataattt ctttagtcat tgcaagtcca atacctgttt
      661 ggccgccatg tcctttatag aagcgatcga aaatataagg caagtcttct tttgaaatgg
      721 ctggtccgtc gtttgagata gttagttttt gatttgtgta agagatttca atatgagaaa
      781 cagcatagcg tagtgcgtta ctaaggatat tattgatagc tcgttgaatc aactcttcgt
      841 taccttttag taacttttca tgcttgtctg gtctataagt gatactaatc ttttgtttat
      901 tagctagggg tttgaaataa gcaataatac tattaagtaa ttcatttaaa gaaaatttat
      961 ctttttgatc acttaagtta ctatctaatt tagagagtaa gataatatcg tctacgagct
     1021 gtttcatttt tttgctctct tgcagaataa cagagtgtgc gagctcggca tcaataatac
     1081 cctcttgaac tccctcagta tatccttgga tggacattaa aggtgtccgt aactcgtggg
     1141 aggcattttg aaagaagttt ctttggctct cttctgcctg gtagattcga tttgccattt
     1201 cttggctctc aagtagaaga ggatcgaaat cagaaaagac aatttctttt tgactagatt
     1261 gatgattgcc ttgatttttc caaaggctgg aaatataagt ttgtactgat tgaatagatt
     1321 ctctaattcc agtgaaagtc ttcctcataa taaatagcat gggaatatag gttagtacta
     1381 agataactat aagaaaatga ttaattttag taattagatg ctgggtgtaa gtaatgtctg
     1441 aaaagttaat cacatgaaaa agttgcgact gtgctttagg agcttttttt atgctaccat
     1501 caacaaggat accagagtag tctttggtca ttacataata ggtttttttc ttgatggtta
     1561 taaacatacc atctttttcc ttggaaattt gccacaatct tttttgggta agatagcgaa
     1621 taatatcatg gctctctttt ttatgattat cagtgccata tttcatacga taatgctggt
     1681 caaaaatagc atagtaagac caaacaaaga tgctatcttg gttaaccata ttttcatctg
     1741 gtattttctt gttgttatag atactatcta aaatggtgaa attcgcctgc atggaattcc
     1801 ttgtcatttg atggacatag tctttaatga catagttgac agaaaaatag atgaggtaaa
     1861 aaccaagaaa tagagtgatg ctattaataa ggataagatt gctaattaaa cttttctttt
     1921 tcatttgtcc tctttgatta atttgaagcc gtaaccccag acagttttga tactaacctg
     1981 actgtggtat tgcttaagtt ttttacgtaa tcgtttaata gtgtcatcaa ctgctctgct
     2041 ttcaatatca tcaaatcccc aaatacgatt aagcagtgtt tccctagaaa aagcagtttc
     2101 aggtttttca attaatacta aaagaaaatc aaattctgtc ttggttaagg gcatttcttc
     2161 atttgataat agggctattc gcttttcagt atccagaatt aaatctagaa ctgatactga
     2221 gtgtcatttt tgattgcttt ttcatcaagg cgtttgaaca atgctttaac atgtagacta
     2281 agttttatag gtgaaaaagg cttggtgaag taatcgtcag ttcctaaatt aaaagccgta
     2341 atgaaatcaa catcagaatc tctagctgtt aacatgataa taggaatatt atcatattgg
     2401 cggatgcttt tcataacatc taaaccatta gttccaggca tcattatgtc taatataact
     2461 aaatcggatt tttggttttt ataggcttct aagagaaggt ctccagtttc aaatgctcga
     2521 acagtgaaac catcatgttc taaaaaagga accaccaagt cacgaatatt cttttcatca
     2581 tcagcgaaat aaattatttt tgtcatgtat acctcaatac ccatagttat aatttgactt
     2641 ttagctagct gtttatgtta tatcaaatat aacaaaaaaa gagttttttt cctattcttt
     2701 gaagtgttat agtgttataa accaagatat tgcagagaaa aggtctttag gaacatcatt
     2761 ttaagctcct caaaaaggca ccagtcacta actattaata gctagtaatt gatgccttgc
     2821 tctatttttt aaagtatttt attctctttt gatagacttt agtttgattg ttgccagacc
     2881 tagcatagag agactaaata gcaatattgt ataggttatt ttaggagtgc tttcgcctgt
     2941 tttaggaagc ttgtttgtct ttatcttttg attatcggtt tggctagtca cccttaagac
     3001 acgcttctga gcaacctcac tttctttagt catgcctttt ttgaatatca acgcctgggg
     3061 ctgatttggc ggattgatag gtgtggtatt gagagagtca ttatcattga gtgattgact
     3121 ataattctct gcggaagaaa ctctatcgcg accatcaagt tcttgttcgg ttgcttttat
     3181 ttggtttaat aactcttcaa cttgagcttg tgtagcttgt ctattggaaa gcagcaattc
     3241 tgctttcttg ataaggttat tataattttc tttagcaaat tgactggcgt tctgatattt
     3301 agatgatgtt tgtttcagaa catttgcttt aattatcata tggtgtaaac cagagtaatc
     3361 agtttcatga ccatttaatt gatttttagc aatatcaaga ttttctaagg ctttgtctac
     3421 ttctgattga gtgacgttag gtttttgtag aagatttttc gccaattgga cttgattgat
     3481 ataagttgat tggcttaatg gactagcatt aacaaattga tagtttccct tgatttctgt
     3541 ttcagctaaa atagcttttg ccagtaaaga agtatctgtt tctttaccgt taagagcttg
     3601 gcgactttct ctcaaagcag caatagcatt atcaatactt gtttgttcca ctctattttt
     3661 aattaaagtt tgtgcctttt caagattagc taggtaatta ttttttaagt gagttccagc
     3721 attataaaac acaggttggg aaataatgtc agtttgttct gctagggcat taattagagg
     3781 agctttatta atcagcgtca attttgttag atttacttga ccttctttta cggatacaag
     3841 taaatcatct aagttctcta aagtgctata cccactaggc agcgttacgt ttaaacgata
     3901 gtctcctaca ggtatgtttt taccatagtc agtaggagaa taagttgctt ttggtagatc
     3961 aacaacagta tttgtcttag taaccaactg gactgttgct ccttttggta aaagcttgtc
     4021 aaattcaacg agtaaggctg ctttcttgag taagttaact ttaaataaga cgtctttaag
     4081 gctatctttt tcagaaatag ttacagtgac actttgggga ctgattagat ttgctcgttc
     4141 ctcatcgtat aagaataggt caaacgtata ggtaccaaaa ggcaatttca gtaaattgag
     4201 gtctttcccg tgatgttgct ttttgatgac attacctttg tcatctttga cagagtaaga
     4261 gaaatcaata tcgacattac tgttaagctc agggctaaac actttgacgt ttactaaacc
     4321 atttttattg ccaatattga tcaaatcttc gagttttgct gacactagat tgcctgcata
     4381 atcttcaaca gtgtagtaga catgagagaa atcattaacc tctttaggta gaataaagct
     4441 accatccttt tcttgtttga taaatacttt gttatcttca acaaggatac cgtcttctcc
     4501 tttttgacgt aagacgctat tataatgacc atctttatct ttttttaagt aaaaaacttg
     4561 ttctctaaag ataccagatt ccccgtgttc tactgcagga cgagctttga agatacgtct
     4621 atctttgtca taagttgctg ttgttagtgt aggagcttgt ctatccaaag tgatatcaaa
     4681 taccatttgt tgtggcttag agccagggac atcagagtag tatgttaaaa cgtatttgta
     4741 ttttccatct tctaaaggaa taccatcagt agtggttcct ttccattctg tgtcatataa
     4801 aaatgtagat tttggatttt cagtatttcc gctgtagtaa tttttatctc ctgcttgggg
     4861 agcacttacc catagtggtt tttgtaaatt aacatcatct gcacgataga ctttggctct
     4921 taaattattg aaattacgta agaaaacacc tttcagtgca acggcatctt gattttggtc
     4981 atcattaggt gaaatggcta gatgaggctg gcctttttgc tctagttgta agatccattt
     5041 tccatctata gacttatagc ttcccaaggc tttcaattgg ataggactag taccgtctgt
     5101 tgagtagata ggctctgaac ttgtagtcat taaggcagta taatcttctg aaccaggaac
     5161 ttcgtttgtt tgtttcggtt ggaaataaaa tcccttttct ttgttagcaa caagcttata
     5221 aatggatttt tctaaaactt ctaagttttg gaattctccc ttaaatccaa cataaggaat
     5281 actaagaact tccccaccat caacagggtc tgtaaaacgt acgtagcctt ctaggaaata
     5341 gccgtttggc attacttttt ttaacatgtc atggtatttc gaaacatcta tatcaattgt
     5401 aatcgtttta gtttgccctg gttcaatccg tatcgttttc cctgtaaatg taccaagttg
     5461 ttgaggagcc aatgtgacaa agccatcttt aacttgatca gtatttaaat aggttgtata
     5521 gtgtaaatct tttgcaactt tattgatatt atgaacagta acatcaaagg aaattttatc
     5581 tttaatattt cctaatgtaa cgctaccata gttgttttcc ccaccagtta ggtataatcc
     5641 tgtctgaact gctgctgcga catcgataat acctgctcct tgttggcgtg gtgaggtgta
     5701 agcgcctgta tctttgttta ggtgtgcttt agcagtactc ataagaaggt atttgacagt
     5761 tctttcaatg tcaccttttt taagttctgg atgttctttc aataagtatt gtttaactag
     5821 agcagtagca ccagcgacat ggggagaagc catacttgtc ccactcatca tatcatattc
     5881 attatcattg atagcggcgt aaatagagcc tccaggagca gataagtcag gttttaattg
     5941 tccgtctgct gttaatcccc agcttgaaaa gtcagatagg acacctgcat tagggttggc
     6001 ttgtttattt ttgatattgt taaatacaat tttatagttg tttttagcta aaacatctcc
     6061 aaactctttt tgagtaaaaa tggcaggaat agcgcttgct tcaggatcta aactcattgt
     6121 tagattagct tctccagctt tgttattaaa gataatagct cccacagcac cgtgattaat
     6181 agcgttgacg actttttttg taaaagtaat atctccacgt tcaattaagg cgattttacc
     6241 atttaaagtt ttgtccttat aatcattgtc gtttcctttt cctacgaaaa cataatcata
     6301 ttgcttccct acttcaaagg tcttatcact aactttagga tctgcataag cagctaatcc
     6361 gttgttgaga tttttgttgt tttctaatcc gataatgttg aagactttat taaccagtgt
     6421 tgtattatta tatgatgcta cagaaatggc ctcacgagct gttgatggac taccaactaa
     6481 accataatca ggataaagag cagaaggctt tgatgctcca ctcccaaatg taccgtcgtt
     6541 acctgctgct ataacaacag aaacgccagc gagtctagcc atctctaaag cttttataag
     6601 tcggtcatcg gcattaacta aagagccatt agctccacct aaacttaaat taatgctatc
     6661 agcaccgagt ttaacggcat cttcaatagc tttaacataa agggctggtc cagttcctct
     6721 tttttcatca gagaagactc tcataaacat tacttgtgct tcaggagcaa caccatagat
     6781 taattcattt gtatctttct tgctaggatt agctgttgcg atactggtta cgtgcatacc
     6841 atgagaagtg cttttcacct cttttagctc tgtattgaca tcaacatagt tgtgaccaaa
     6901 gattactttg ttgttatacc attttccata gtttatacca gcttttgctt tagcagcatt
     6961 catttgctgt tcgttttggt attttgcttt tgtcgaatca tttaattgga gagcatcgtg
     7021 attggtatct agtcctgagt caataattgc tacaacagtt ccctctcctt tgtagcctgt
     7081 gttccatact ttggggacag tgataatttt attactatca atgttttgag gggtttcttt
     7141 agaaatagct ggtgtaggtt tgactgctaa attttgtttg gttaaaggag ttttagaaac
     7201 ttcttgagtg ctttcaaccg gttgatttgc ggctggggaa aatgtctcgg tggcttgaga
     7261 gtgaggctca gttactggtt gtgtttctgt tggactatta ttattagcct ttgtaacaag
     7321 attgtcttga gctgttgacg caagggctga tgacgtgttg ttagtaggtg tctcatttgc
     7381 tatgctcgtt ggactgtctt ttacttgctg ttcaactgca atatcagatg gcttatttat
     7441 gacagaatca gcagctacat ttgtgatacc gcctaaaaaa aatagggttc ccaaaagtac
     7501 agatacggca cctaacttat atttccggat tgaaaaacgc tgttttgtgt tcataaaaat
     7561 ctccttttct tgtaacataa taattacaaa aactattaaa acataaaaaa tcaaaatttt
     7621 ctacaatttt ctgaattttc attacaaatt attttgtggt caatttaaga ccgtacctaa
     7681 agcatttttt aaaaatcatt ctatgaaaat aataatattg tccaactata tggctacaat
     7741 atcaaaggtt tatagaataa ttacaggata tttattagtc taattgctgg caaagctaaa
     7801 taatcattcc tcttctttct tagtaatcac cgttgtgcta taataactga atatactgaa
     7861 aatttagggg gatatgatgg aaaaaggatt taaagaggga gtgaaagatg ccttgcccac
     7921 agctttaggg tatatctcaa taggcttggc gtttgggata gttgcttcag catcagattt
     7981 atctgcaata gaagttggtc ttatgagtgc cttggtatat gggggatctg ctcagtttgc
     8041 tatgtgtgcg ctattattag caaaagcaga cttgatgact attacgatga cagtattttt
     8101 agttaatttg agaaatatgt taatgagttt acacgcgact actatcttca agtctgctca
     8161 cctaatgaat caattagcca taggaactct aataacagat gagagctacg gcgttttact
     8221 tggagaagca ttgcatcaca aagtcgtatc gcctagttgg atgcatggta ataatgtgat
     8281 gagttatttg acatgggtaa tctcaacgat aataggaact ttactgggga gtactattcc
     8341 taatccagaa atgtttggtt tagactttgc attagtcgcc atgtttatag gtctctttgt
     8401 atttcagttg tttgggatgt tatctgatgg aaagcgactt gttgtatatg ttcttgcttc
     8461 tgtaggttta agttacttct tattatccac ttttttgtca ggagccttat cagtattgct
     8521 tgcgactgtt gttggatgta gtgtaggagt tgttttagat gataaatagt accgtttttc
     8581 ttgctattct tttaggtttt gttgtttctt gggtgccaag agtcgctcca ttcatatttg
     8641 taaaatacaa agggctacca aatagtcttg tgaaattttt aaaatttctt cctctttcta
     8701 ttttatttgc cctaacattc tctagctttt tttcagttaa attaggacaa ttgccaacac
     8761 taaaatggtt agatttattg gctggcatac caactctagt tgtcactatt cgatatcgaa
     8821 atttatttta cactgttctt actggtatcg ttactatggc tttattgaga ttactatttt
     8881 aacgtgtagg aatggaagat taataaaaac acaaaaaacg catctatttc tagatacgtt
     8941 ttttgtgttt ttgtctagac aggaactttt gatagccgat gtgtaatagt ttgactaatt
     9001 gttaataata tcaacagatt tttctgtatc ttgataatat gagttgattg taagattgct
     9061 ttttaaagat atctacatgc tgctatttta aaatttataa a
//
LOCUS       DATROQ010000074         8846 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.075, whole genome shotgun sequence.
ACCESSION   DATROQ010000074 DATROQ010000000
VERSION     DATROQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8846
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.075"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..1188)
                     /locus_tag="VBC94_001576"
     CDS             complement(<1..1188)
                     /locus_tag="VBC94_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface protein"
                     /protein_id="HEO6979834.1"
                     /translation="MSKKHIFICLFFMLFLVGCQNNKKVKTDKKVDITAYYYHNNTPQ
                     YSVQINAIHLQTALDKVRTGEFESKDFTHYTFDSIKRKYQVSGKKVLMDNNYRFGSGI
                     KNTRQDAIAIVRLLLEKNKDYLIYMNGLEEPSVLYNPENKRYKFTNNKGKAIGNIPVG
                     LTAFENSAETQEYVLKNIQKNETIYLGNTRVDNPRVTVNNKKRDTIGVEYGKRITYRI
                     PIYSKQLTVRVSPNFVVDSTNYNYRLSQAPIIAEEGKEGRLYPIDSSISVDGDKIVLN
                     SSGDETEQKELLQNSLYKLHSIDKLDFDLTNADIKELEITGHVASKRDYNLSVAQEKK
                     GVIETKRSLISVDNQKQDQGIFVEDSKGYIKTPSLSSYGINFAMYDDNQNRLLQGSEY
                     ILGR"
     gene            complement(1181..1534)
                     /locus_tag="VBC94_001577"
     CDS             complement(1181..1534)
                     /locus_tag="VBC94_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEO6979835.1"
                     /translation="MSLALQIEMFILAIVLLYMIIRMVNKNTFSVKRSLPWLLVGLIL
                     VFISVFPGVVSFIAHHLGFALTMNFLLFGAILFLFCLELFDTSSNTKRDEQIKRLIQE
                     VSLLKKEIEDSTLDE"
     gene            complement(1531..2262)
                     /locus_tag="VBC94_001578"
     CDS             complement(1531..2262)
                     /locus_tag="VBC94_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6979836.1"
                     /translation="MMKKILLVIPAYNEEGSIAKTVQTIVDFKASRSLPFELDYIVIN
                     DGSTDGTPELLDRLGLNHIDLVQNLGIGGCVQTGYLYANRNHYDVAVQFDGDGQHDIR
                     SIEDVVMPILNDEADFVIGSRFVDKKHQNFQSTAMRRLGINLISAAIKLTTGHKVYDT
                     TSGYRAANAALIAYLSCHYPVQYPEPESTARILKKGYRLKEVTANMFEREAGTSSISS
                     LKSIFYMTDVLTSIIIAGFIKEDDK"
     gene            complement(2264..3205)
                     /locus_tag="VBC94_001579"
     CDS             complement(2264..3205)
                     /locus_tag="VBC94_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6979837.1"
                     /translation="MKVNILMATYNGEKFLAQQIESIQKQTFKEWNLLIRDDGSSDKT
                     CDIIRNFTAKDSRIRFINENEHHNLGVIKSFFTLVNYEVADFYFFSDQDDVWLPEKLS
                     VSLEAAKHKASDVPLLVYTDLKVVNQELNILQDSMIRAQSHHANTTLLPELTENTVTG
                     GTMMINHALAEKWFTPNDILMHDWFLALLAASLGEIIYLDLPTQLYRQHDNNVLGART
                     MDKRFKILREGPKSIFTRYWKLIHDSQKQASLIVDKYGDIMTANDLELIKCFIKIDKQ
                     PFMTRLRWLWKYGYSKNQFKHQVVFKWLIATNYYNKR"
     gene            complement(3195..4349)
                     /locus_tag="VBC94_001580"
     CDS             complement(3195..4349)
                     /locus_tag="VBC94_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEO6979838.1"
                     /translation="MQDVFIIGSRGLPARYGGFETFVSELINHQKSSDIKYHVACLSD
                     KEHHTHFNFADADCFTINPPQLGPARVIAYDIMAINYALDLVKTHDLKEPIFYILGNT
                     IGAFIWHFANKIHKVGGLLYVNPDGLEWKRSKWSRPTQRYLKYAEKCMTKNADLIISD
                     NIGIENYIQSTYSNVKTRFIAYGTEINSRKLSSDDPRVKQLFKKWNIKSKGYYLIVGR
                     FVPENNYETAIREFMASDTKRDLVIICNHQNNPYFEKLSLKTNLQQDKRVKFVGTLYE
                     KDLLDYVRQQAFAYIHGHEVGGTNPGLLEALANTDLNLVLDVDFNKSVAGLSSFYWTK
                     KEGDLAKLINDSDQQQDLSTYGDRAKAIIQENYTWKKIVEEYEDLFLNES"
     gene            complement(4466..5320)
                     /gene="rfbD"
                     /locus_tag="VBC94_001581"
     CDS             complement(4466..5320)
                     /gene="rfbD"
                     /locus_tag="VBC94_001581"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEO6979839.1"
                     /translation="MILITGANGQLGSELRHLLDERTQEYVAVDVAEMDITNAEMVDK
                     VFEEVKPSLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKAAAKHDATLVYISTD
                     YVFDGEKPVGQEWEVDDLPDPKTEYGRTKRMGEELVEKYTSKFYTIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQHGRPTWTRTLAEFMTYLAENQKDFGYYHLSNDAKEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLEKAKATGFVIPTWQDALKE
                     FYKQEVKK"
     gene            complement(5410..5751)
                     /locus_tag="VBC94_001582"
     CDS             complement(5410..5751)
                     /locus_tag="VBC94_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEO6979840.1"
                     /translation="MSEVKQYSEEEVGKIKDRILEALEMVIDPELGIDIVNLGLIYEI
                     RFEDNGRTEIDMTLTTMGCPLADLLTDQIHDVMKTVPEVTETEVKLVWYPAWSVDKMS
                     RYARIALGIRS"
     gene            complement(5860..6969)
                     /gene="rpoD"
                     /locus_tag="VBC94_001583"
     CDS             complement(5860..6969)
                     /gene="rpoD"
                     /locus_tag="VBC94_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019787874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEO6979841.1"
                     /translation="MAEKKGNTTFNVQVADFIRNHKKQGTAIDDEVTEKLVIPFVLDA
                     DQIDDLLERLTDGGISITDKEGNPSTKYVVEGPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELAVAVAEGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLKDFMED"
     gene            complement(6977..8785)
                     /gene="dnaG"
                     /locus_tag="VBC94_001584"
     CDS             complement(6977..8785)
                     /gene="dnaG"
                     /locus_tag="VBC94_001584"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO6979842.1"
                     /translation="MGYFCGGHDLAIDKEKISEIKNSVNIVDVIGEVVGLTKTGRNHL
                     GLCPFHKEKTPSFNVIEDRQFFHCFGCGRSGDVFKFVEDYQHISFLDSVQVLAERSGI
                     PLDTNFKGQVPKKPKANQSLLDIHRVASGFYHAYLMTTNDGERARQYLAERGVTEDLI
                     KHFQIGLSPGGQDFLYRRLAKEFDEKTLMSSGLFNYSENSNQFYDSFNNRIMFPLTND
                     IGEVIAFSGRVWTQEDIDRKQAKYKNSRATPIFNKSYELYHLDKARAVINKAHEVYLM
                     EGFMDVIAAYRAGIENVVASMGTALTNEHVRHLKRFTKKVVLTYDGDRAGQNAIDKSL
                     ELLSDMTVDIVRIPNKMDPDEFLQANSAEDFKQLLENGRISNTEFYIHYLKPENTDNL
                     QSEIAYVEKIAKLIAKSPSITAQNSYITKVAELLPDFDYFQVEQSVNNERLHHRSQQQ
                     ASSSVQTSATVQLPQTGKLSAITKTEMQLFHRLLNHPYLLNEFRNRDNFYFDTTEIQV
                     LYELLKESGEITSYDLSQESDKVNRTYYIILEEQLPVEVSIGEIEAVEKARDRLLKER
                     DLRKQSQLIRQSSNQGDEEGALAALENLIAQKRNME"
BASE COUNT         2760 a         1776 c         1290 g         3020 t
ORIGIN      
        1 tcttcctaaa atatactctg aaccttgcaa taatctattt tgattgtcgt catacatggc
       61 aaaatttata ccataactgc tcaaactagg tgtctttata tagcctttag aatcctcgac
      121 aaaaatccct tgatcttgtt tttgattatc aacagaaatt agactccgtt tagtctctat
      181 gacgcctttc ttttcctgag caacagacaa attataatct ctttttgaag caacatgtcc
      241 tgtaatctct aactctttaa tatcagcatt tgtcaaatca aagtctaact tatcaataga
      301 gtgaagttta tacaggctat tttgtaataa ttctttttgc tctgtttcat caccactact
      361 atttaaaaca attttatcac catcgacact aatactgcta tctattggat acaaacgtcc
      421 ctctttacct tcttccgcaa taataggcgc ttgagaaagt cggtaattat aattagtact
      481 atctactaca aaattagggc taactctgac agtcagctgc tttgaataaa ttggaatcct
      541 ataagtaata cgtttaccgt attcaactcc tatagtatct ctttttttgt tattaacagt
      601 tactcgagga ttatctactc gggtattacc aagataaatc gtttcgtttt tctgaatatt
      661 tttcaacaca tattcttgag tttctgcgct gttttcaaaa gcagttaaac caactggaat
      721 attaccaatt gcttttcctt tgttatttgt gaacttataa cgtttatttt ctggattata
      781 taaaacactt ggctcttcta agccattcat gtaaattaaa taatctttat tcttttcaag
      841 cagtaacctt acaatagcaa tagcatcctg tctagtattt tttataccag aaccaaatct
      901 atagttatta tccataagga cttttttacc actaacttgg tacttccgct tgatactatc
      961 aaaagtatag tgtgtaaagt ctttgctctc aaactccccc gtcctcactt tatccaatgc
     1021 tgtttgtaaa tgaatagcat taatctgaac actgtattgc ggggtattat tatgataata
     1081 ataagctgta atatctactt ttttatctgt ttttactttt ttattatttt ggcaacctac
     1141 taaaaaaagc atgaaaaaca gacaaataaa aatatgtttt ttactcatct aaagtgctat
     1201 cctctatctc ttttttcaac aaagaaacct cttgaatcag gcgtttaatt tgctcatctc
     1261 ttttggtatt gctactagta tcaaataatt ctaaacaaaa cagaaataaa atagcaccaa
     1321 ataacaagaa attcatcgtt aaagcgaatc caaggtgatg agcgataaat gagaccaccc
     1381 caggaaatac actaataaag actagaatta aaccaactaa tagccaaggt aaagaacgtt
     1441 taacagaaaa tgtgttttta ttaaccattc ttataatcat ataaagtagg acaatggcta
     1501 aaataaacat ttcaatttgc aatgctaaac tcatttatca tcctccttaa taaaccctgc
     1561 aataataatt gatgttaaaa catccgtcat ataaaaaatc gattttaggg aacttatact
     1621 tgaagtacct gcctcacgtt caaacatatt agctgtgact tccttcaaac gatagccttt
     1681 tttcaaaatc cgagcagttg attcaggctc tgggtattgt actggataat gacacgataa
     1741 ataagctatt agcgccgcgt tggctgccct ataaccactt gttgtatcat agactttgtg
     1801 tcccgtcgtt aattttatag cagcagatat taaatttatg ccaagacgcc tcattgctgt
     1861 cgattgaaaa ttttgatgtt ttttatcaac aaagcgagaa ccaataacaa aatctgcttc
     1921 gtcatttaag attggcataa caacatcttc aatgcttcta atatcatgtt gaccatcacc
     1981 atcaaattga acggcaacat cgtaatgatt acgattagcg tataagtatc ctgtctgcac
     2041 acagccacca atacctagat tttgaacaag gtcaatatga tttaaaccca atctatctaa
     2101 taactctgga gtaccatcgg ttgacccatc gttaataaca atataatcca gctcaaaagg
     2161 tagtgagcga gacgccttaa aatctacaat agtctggact gtctttgcaa tactaccttc
     2221 ttcattataa gcaggtatca ctagtaaaat ttttttcatc atcttatctc ttattgtaat
     2281 aatttgttgc aatcaaccat ttaaacacaa cttgatgctt aaattgattt ttgctataac
     2341 catatttcca taaccacctt aatcttgtca taaaaggctg tttgtcaatt tttataaaac
     2401 atttaatgag ctcaagatca ttagctgtca taatatcacc atacttatca acaattaagg
     2461 aagcttgctt ttgtgagtca tggataagtt tccaatacct tgtaaatata gatttaggac
     2521 cttcacgaag tatcttaaat ctcttatcca ttgtcctcgc ccccaaaaca ttattgtcat
     2581 gttgacggta tagttgagtt ggtaaatcca aataaatgat ttctcctaaa cttgctgcta
     2641 agagtgctaa aaaccaatca tgcattaaga tatcattagg agtaaaccac ttctcagcta
     2701 atgcatgatt tatcatcata gtcccaccag taacagtatt ctcagttagt tctggtaata
     2761 atgtcgtatt ggcatggtgc gattgagccc gaatcatgct gtcttgtaaa atatttaatt
     2821 cttgattaac aactttcaaa tcagtataaa caagtaatgg tacatcagat gctttatgct
     2881 ttgctgcctc taaacttaca gataactttt ctggcaacca tacatcatct tgatcactaa
     2941 aaaaatagaa atctgcaact tcgtaattaa ctagcgtaaa aaatgattta ataactccta
     3001 aattatggtg ctcattttca ttaatgaaac gaattcgaga atctttagca gtaaagtttc
     3061 taataatatc acatgtcttg tcacttgaac catcatctct gattaggagg ttccactctt
     3121 taaaggtttg cttttggata ctttcgattt gctgcgccag aaacttctct ccattgtagg
     3181 tggccattaa aatattaact ttcatttaaa aacaaatcct catattcttc aacaattttt
     3241 ttccaagtat agttttcttg aataatggct tttgctcgat caccgtaagt agataaatcc
     3301 tgttgttgat cactatcgtt aataagctta gctaaatccc cctctttttt agtccagtaa
     3361 aaacttgaga gacctgctac tgatttgttg aaatcaacat ctagaacaag attcaaatca
     3421 gtattagcta aagcctcaag cagtcctgga ttagtaccgc caacttcatg cccatgaata
     3481 taagcaaagg cttgttgacg aacataatcc agcagatctt tttcatagag cgtacctaca
     3541 aacttaactc ttttatcttg ttgaaggttt gtctttaagg acaacttttc aaagtagggg
     3601 ttattttgat ggttacagat aataactaaa tcacgcttag tatctgaagc catgaactcc
     3661 ctaatagccg tttcataatt gttttcaggg acaaatcgac caacgattag atagtaaccc
     3721 ttagacttaa tattccattt tttaaacaac tgtttgacac gtggatcatc tgacgataat
     3781 ttcctagaat taatctctgt accgtaagca atgaaccttg tcttcacatt agagtaggta
     3841 gattgaatgt aattttcaat accaatatta tcagaaataa ttaggtctgc atttttagtc
     3901 atacattttt cggcgtattt taaataacgc tgtgtgggac gagaccactt tgatcgcttc
     3961 cactctaaac catccggatt aacatacaat aagccaccga ctttatgtat tttattggca
     4021 aaatgccaaa taaaggcacc aattgtattt cctaaaatat aaaaaatagg ctcttttaaa
     4081 tcatgtgtct taaccaagtc aagggcataa ttaatggcca taatatcata agcaatcaca
     4141 cgtgctggcc ctaattgggg aggatttata gtaaaacaat cagcgtcagc aaagttaaaa
     4201 tgagtatgat gttctttatc actaaggcat gcaacatggt attttatgtc ggaacttttt
     4261 tgatgattaa tcaattctga aacaaaagtt tcaaaaccac cgtaacgagc tggcaaccct
     4321 ctacttccta taatgaaaac atcttgcatc atcttctcca ttctagctaa acaatccata
     4381 caattatacc ataaattaga aaagttagct aaaaagagca gtaagctaaa aggattctct
     4441 agccttacta ctctttgttt tgatcttatt ttttaacttc ctgcttgtag aactctttta
     4501 aagcatcttg ccaagtagga atcacaaatc ctgttgcttt cgctttttcc aaactcatag
     4561 ttgaatttaa aggacgtttt gcttttgctg ggaattgact agaatcaaca ggcttaacct
     4621 ccacatctgt gtcttttaag atttcaaccg caaaatcata ccatgttgta tcttctttag
     4681 catcatttga taaatgatag taaccaaaat ctttttgatt ttcagctaaa taagtcataa
     4741 actcagccaa agtacgtgtc catgttggtc gtccatgttg gtcgttaaca acagttagag
     4801 ttttgtgtgt ttttgcaagg ttttgcatcg taaagacaaa gtttttacca tagttaccaa
     4861 atacccacgc tgtgcgaatg gtatagaatt ttgaagtata tttttcaact aactcttcac
     4921 ccatacgttt tgtacgaccg tactctgttt tgggatcagg cagatcatct acttcccatt
     4981 cttgccccac tggtttttca ccatcaaaca cgtagtccgt cgaaatgtaa acaagtgttg
     5041 catcatgttt agcagcagcc ttagcgacat tctctgtacc agtgacattg attgcaaagt
     5101 ctagctcttt tccttcatct tctgccgcat caacagctgt gtaagctgca caatggtaaa
     5161 ctaaagatgg cttcacctct tcaaagactt tgtccaccat ctcggcattt gtaatatcca
     5221 tttcagctac atcaacagca acatactctt gagttcgttc gtctaaaagg tgacgtaatt
     5281 cactacctaa ttgcccgttt gcccctgtaa ttaaaatcat tccttgctcc tttaaaaata
     5341 agtttaactt ttaattcttc gtttattgta cactaaaaaa gggaatattt catcccttta
     5401 ctctttatct tatgaacgaa ttcccaaagc gatacgagca taacgactca tcttatcaac
     5461 agaccaggct gggtaccata ccaatttcac ctcggtttca gttacctctg ggactgtttt
     5521 cataacatca tgaatttgat ctgtcaataa atctgctaac gggcatccca tagtcgttaa
     5581 agtcatatca atttcagttc gaccgttatc ttcaaagcga atctcataaa ttaacccaag
     5641 gttaacaata tctattccca attcaggatc gataaccatc tccaaagctt ctaggatacg
     5701 atcttttatt tttccaactt cttcttcgct atattgcttt acttctgaca ttataatacc
     5761 tctctgaaaa tattaatacc gtctttaaga taactccttt gcaaatcatt tttattagaa
     5821 actaaacttc ctattctaaa aacgattatc gagaagcaat caatcttcca tgaaatcttt
     5881 aagttgcttg ctacgactag gatggcgaag ttttctaagg gctttagctt caatctgacg
     5941 aatgcgctca cgagtaacat taaatacttt acctacgtcc tctaacgtac gcattttacc
     6001 atcgtcaaga ccaaagcgta aacgaagaac attctcttca cggtcagtaa gagtatctaa
     6061 aacctcatct aattgctcac gaagaacaac acgtgtagta taatctactg gattttcaat
     6121 aacttcgtct tcaatgaaat cacccaaatg gctatcatct tcctcaccga ttggcgtttc
     6181 tagcgatacc ggctcttgag caatttttag gatttcacgt actttatctg gtgtcatatc
     6241 catgcgttct gcgatttgct caggagttgg atcttgacca agttcttgaa gaagattacg
     6301 ttgttcacgg accaatttat taatcgtttc aaccatatga acaggaatac gaatcgtacg
     6361 tgcttgatct gcaattgcac gtgttatagc ttgacgaatc caccatgttg cataagttga
     6421 aaatttgaat ccctttgaat aatcaaattt atcaacagct ttcatgagcc ccatgttacc
     6481 ctcttgaatt aaatcaagaa attgcattcc acgtccaaca taacgttttg caattgagac
     6541 aacaagacga aggtttgctt cagctaaacg ttgtttagcc attaaatctc cctcagcaac
     6601 cgcaacagcc aattcttttt cttcttcatt cgttaaaaga ggaacaacac caatttcctt
     6661 caagtacata cgtactgggt cattgacttt agcagaatta cttcctatga gctcttcatc
     6721 agtcaattct tcaggttttg gcccttctac cacatacttt gttgaaggat tgccttcttt
     6781 atctgtgatt gaaatgccac cgtctgtcaa tcgttctaac aaatcatcaa tttgatctgc
     6841 atctaaaaca aaaggaataa caagtttttc tgttacctca tcatcaattg cagtaccttg
     6901 ttttttatga ttgcgaataa aatccgcaac ttgaacatta aaagttgtat ttcctttttt
     6961 ctctgccata ggttcctcat tccatatttc ttttttgagc aattaaattt tctaatgcag
     7021 ccaaggctcc ttcttcatca ccttgattac tagattgtct aattaactgg ctttgttttc
     7081 ttaaatcacg ttctttcaat aaacggtcac gtgccttttc gacggcttca atctctccaa
     7141 tagatacttc tacaggtaat tgttcctcta aaattatata ataagtacga ttaactttgt
     7201 cactttcttg tgataaatca taagacgtga tctcaccact ttcttttagc aactcatata
     7261 aaacttgtat ttctgttgta tcaaaataaa aattatccct attacggaat tcatttaaca
     7321 ggtagggatg attcaataat cgatgaaaca attgcatttc tgttttagtg atagctgata
     7381 acttacctgt ctgtggtaat tgcactgtag cacttgtttg tactgaagat gaagcttgct
     7441 gttgacttct atgatgcaat cgttcgttat taaccgattg ttctacttga aaataatcaa
     7501 aatctggcaa gagttcagcc acttttgtaa tatatgaatt ttgtgctgtt atcgatggag
     7561 atttagcaat taatttcgct attttttcaa cataagcaat ttcagattgt aaattatcgg
     7621 tgttttcagg ctttaagtag tggatataaa actctgtatt actgatgcgc ccattttcta
     7681 agagttgttt aaaatcttct gctgaatttg cttgtagaaa ttcatcaggg tccattttat
     7741 taggtatcct aacgatatca acagtcatat ctgatagcag ttctaaagac ttgtcaattg
     7801 cgttttgacc agctctatca ccatcataag tcagaacaac tttcttagta aatcttttaa
     7861 ggtgtctaac gtgttcattg gttaaagcgg tccccataga agcaacaaca ttctcaattc
     7921 ctgcacgata agctgcaata acatccataa agccttccat taggtaaact tcgtgtgctt
     7981 tattaattac agcacgcgct ttatctaaat ggtaaagctc gtaagactta ttaaaaatag
     8041 gggtggcacg agaattttta tatttagctt gttttctatc aatgtcctct tgagtccaaa
     8101 ctcgtcctga aaacgcaatg acttcaccaa tatcattagt taacggaaac ataattctat
     8161 tattaaatga atcgtaaaac tgattgctat tttctgaata attgaacaaa ccagaagaca
     8221 tcaaagtttt ttcatcaaac tcttttgcca gacgacgata tagaaaatcc tgccctcccg
     8281 gcgataatcc tatttgaaaa tgtttaatta agtcctctgt cactccgcgc tcggcaaggt
     8341 attgtctagc gcgttcacca tcattagttg tcattaaata ggcatggtaa aatcccgaag
     8401 caactctatg aatatctaaa agcgactgat tagctttagg ttttttagga acttgcccct
     8461 taaaattagt atctagaggt attcctgaac gttcagctag aacttgtaca ctatctaaaa
     8521 aactaatatg ttggtaatct tccacaaatt taaagacatc accagagcga ccacaaccaa
     8581 aacaatgaaa aaactgccta tcttcgataa cattaaaaga tggcgttttt tctttatgaa
     8641 atgggcataa accaagatga ttgcgtcctg tttttgtcaa accgacaact tcaccgatga
     8701 catcaacaat gttaacactg ttcttgattt cagaaatttt ttctttatca attgccaagt
     8761 catgacctcc acagaaataa cccattatag ttttacattt gattatatca ttttttcttt
     8821 tctgtgtaaa ttttttcaaa agcttt
//
LOCUS       DATROQ010000075         8553 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.076, whole genome shotgun sequence.
ACCESSION   DATROQ010000075 DATROQ010000000
VERSION     DATROQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8553
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.076"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..868
                     /locus_tag="VBC94_001585"
     CDS             <1..868
                     /locus_tag="VBC94_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEO6979843.1"
                     /translation="IANPHAGNKNASTIVGKIQELYHTEDISVFYTEQKDDEKKQVIN
                     ILRSFKESDHLMIIGGDGTLSKVMTYLPQHIPCAYYPVGSGNDFARALKIPNLKETLT
                     AIQTERLKEINCFIYDKGLILNSLDLGFAAYVVWKASNSKIKNILNRYRLGKITYIVI
                     AIKSLLHSSKVQVLVEGETGQRIKLNDLYFFALANNTYFGGGITIWPKASALTAELDM
                     VYAKGHTFLKRLSILLSLVFKRHTTSKSIKHQTFKAMTVYFPKNSLIEIDGEIVELDQ
                     ISLKCQKRYLYM"
     gene            878..1075
                     /locus_tag="VBC94_001586"
     CDS             878..1075
                     /locus_tag="VBC94_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEO6979844.1"
                     /translation="MYQVGSLIEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            1278..1394
                     /locus_tag="VBC94_001587"
     CDS             1278..1394
                     /locus_tag="VBC94_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979845.1"
                     /translation="MDNLIRLAEIFDVSLDEHVLAKTNEVKVERIYEKNLDL"
     gene            1638..2753
                     /gene="ychF"
                     /locus_tag="VBC94_001588"
     CDS             1638..2753
                     /gene="ychF"
                     /locus_tag="VBC94_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEO6979846.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEEEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ADPDDIDYVNQIRAFAETESAEVVVISARAEEEISELDDEDKLEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LIQYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2837..3412
                     /locus_tag="VBC94_001589"
     CDS             2837..3412
                     /locus_tag="VBC94_001589"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEO6979847.1"
                     /translation="MVKMIVGLGNPGSKYNDTKHNIGFMAVDRIVKKLDVNFTEDKNF
                     KAEIGSDFINGEKIYFIKPTTFMNNSGIAVKALLTYYNISIKDMIIIYDDLDMEVGKI
                     RFRQKGSAGGHNGIKSIIAHLGTQEFDRIKVGIGRPNGRMTVINHVLGKFDKNDEIMI
                     SNTLDKVDNAVNYYLQTNDFQKTMQKYNGLK"
     gene            3409..6906
                     /gene="mfd"
                     /locus_tag="VBC94_001590"
     CDS             3409..6906
                     /gene="mfd"
                     /locus_tag="VBC94_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEO6979848.1"
                     /translation="MNIIELFSQNKVVRTWHSGLVTNSRQLVMGFSGASKAIAIASAY
                     EKLSKKIMVVTATQNDSDKLSSDISSLIGEDNVYQFFADDVPAAEFIFSSLDKSISRL
                     SALRFLKDPEKNGVLITSISGLRLLLPNPEVFSKSQYKFEIGQECYLDKLSKNLVNLG
                     YQKVSQVFSPGEFSQRGDILDIFEMTQEYPYRLEFFGDEIDGIRQFDIDTQKSLKQLE
                     SVQISPADDIILQDADFERAKKKLEGYLVTASEEQRAYLSEVLSTTENHFKHSNIRRF
                     LSIFYEKEWGILDYIPEGTPLFVDDFQKIVDRNAKLDLEIASLLTEDLQQGKSHSSLN
                     YFSDPYKQLRQYQPATFFSNFHKGLGNLKFDKLHHFTQYGMQEFFNQFPLLVDEINRY
                     KKSGATVLLQVDSQKGLNLLQENLKEYGLDLIISDKNDIVPKESQLIVGHLSNGFYFA
                     DEKIVLITEREIYHRRVKRKIRRSNISNAERLKDYNELSVGDYVVHNVHGVGKFLGIE
                     TIEIQGIHRDYLTIQYQNADRISIPVEQIELLTKYVSADGKEPKINTLNDGRFKKAKQ
                     RVAKQVEDIADDLLKLYAERSQLQGFAFSPDDNMQNDFDNDFAYVETEDQLRSIKEIK
                     QDMEGNRPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHFENFKE
                     RFSNYPVTVDVLSRFRSKKEQTDTLKRLSKGQVDIIIGTHRLLSQDVVFSDLGLIVID
                     EEQRFGVKHKEKLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLETNPGLVREAIIREIDRGGQVFYVYNKVDTIEQKVSELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFVENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEISEKRLDAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIATKQGKSLIRQKGNVELALQIDAYLPAEYISDERQK
                     IEIYKRIRELETRADYEALQDELIDRFGEYPDQVAYLLEIGLLKAYLDLAFTELVERK
                     GNEISIIFEKASLKYFLTQDYFEALSKTQLKARISETNGKMEVVFNIKHKKNYEIIEE
                     LLKFAECFIEIKSRKPVEE"
     gene            7197..7469
                     /locus_tag="VBC94_001591"
     CDS             7197..7469
                     /locus_tag="VBC94_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEO6979849.1"
                     /translation="MRLDKYLKVSRIIKRRPVAKEVADKGRVKVNGVLAKSSTDLKLN
                     DQVEIRFGNKLLTVKVLEMKDSTKKEDAIKMYEIINETRIETDEQA"
     gene            7456..7827
                     /locus_tag="VBC94_001592"
     CDS             7456..7827
                     /locus_tag="VBC94_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEO6979850.1"
                     /translation="MSKPNVVQLNNQYINDENLKKRYEAEELRRKNRLMGWVLIFVML
                     LFILPTYNLVKSYRTLQERRQEVVKLTKDYQTLTNRTENQKLLAKQLKNPDYVQKYAR
                     AKYYFSKTGEMIYPLPDLLPK"
     gene            7830..7964
                     /locus_tag="VBC94_001593"
     CDS             7830..7964
                     /locus_tag="VBC94_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEO6979851.1"
                     /translation="MNNLIETIEHFLAYSDDKLEELADKNYQLRQTIVEKEKIESREK
                     "
     gene            7964..>8553
                     /locus_tag="VBC94_001594"
     CDS             7964..>8553
                     /locus_tag="VBC94_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6979852.1"
                     /translation="MKKVLTFLLCSLYFVSIPAISTEEPLTLSQNRRYALTQTVVDKE
                     MYFDAIPERPTTKIEISSFQDEALTITGETLVPNTLLSIISLTINSNGIPVFTLSNGQ
                     FIKASREAIFNDLVSKQQSVSLDYWLKPSFVTYEAPYTNGVSEVKNNLKPYSRVHLVE
                     QAETEHGIYYKTDSGFWISVEDLSVADNRMAKVQEV"
BASE COUNT         3030 a         1179 c         1628 g         2716 t
ORIGIN      
        1 aatagctaac ccccatgctg gtaataaaaa tgcctccact attgttggta aaattcagga
       61 gctttatcat actgaagata tttctgtgtt ctatacagaa cagaaagatg atgaaaaaaa
      121 acaagtcatt aatatactaa ggtcttttaa agaaagtgat catctaatga ttataggagg
      181 agatggtacc ttatcaaaag taatgactta tctcccccaa catattccgt gcgcttatta
      241 tcctgttggt tcaggaaatg attttgccag ggctttgaaa attcctaacc tcaaagaaac
      301 ccttaccgct attcagacag aaagattaaa ggaaattaat tgctttattt atgataaagg
      361 gctgattttg aatagtttag atttaggttt tgcagcctat gtcgtttgga aagcatctaa
      421 ctctaaaata aaaaatattt tgaatagata tcgtctagga aaaataactt acattgttat
      481 agctatcaaa tcattattac attcttctaa agtacaagta ttagtggaag gagagacagg
      541 acagcggata aaactaaatg acctttattt tttcgcatta gcaaataaca cttattttgg
      601 aggtggcatt actatttggc ctaaagcaag tgctttaact gcagagttag atatggttta
      661 cgctaaaggg catacttttc taaaacgatt aagtatttta ttatctttgg tttttaaaag
      721 gcatactacc tctaaaagta taaaacacca gactttcaaa gctatgaccg tatattttcc
      781 taaaaatagt ttgattgaaa tagatggcga aatagttgag ttagatcaga ttagtttaaa
      841 atgtcaaaaa aggtatttat atatgtaagg agaaaagatg tatcaagttg gatcacttat
      901 tgaaatgaaa aaacctcatg cttgtgtaat taaagaaact ggtaaaaagg ctaatcaatg
      961 gaaggtactg agagtaggag ctgatattaa aattcagtgt actaactgtc agcatgtgat
     1021 tatgatgagt cgttatgatt ttgagcgaaa attaaaaaaa gtactgcaac catgaaagtc
     1081 cttttaaaca aataattata gctatatttg caaccaatac tttcctaaat aattgctagt
     1141 atgccgttgg aaaattagct attctaatgt tatcgaaaga agaaaggtgg ccattgacaa
     1201 tgacgaaagt tgcagaacag ttgtttattt ttcggtaagt catatcaaaa tgggaaaatg
     1261 gggataccac accagatttg gataatttaa tcaggttagc tgagattttt gacgtgagct
     1321 tagatgaaca tgttttagct aaaacaaatg aagttaaagt tgaacgtatt tatgaaaaaa
     1381 atcttgatct gtaaaaatat aataaaattt attggtttat ttttcgaaat attattcgat
     1441 ctctactaat tatcttagct atattaactt tcttataagc tctaggtctt ccctttgttt
     1501 cggagtggat aatttaaatg aaggtgaaag agtggcataa tgataagcaa atctgaaatt
     1561 ttcttcttat cataccgctt tttcttctat tttttgatat aatggtcttg attgaaattt
     1621 gaatggagac tcactaaatg gctttaacag caggtattgt aggcttacca aatgttggta
     1681 aatcaacttt atttaacgca attacaaaag caggagcaga agctgctaac tatccttttg
     1741 cgactattga tcccaatgtt ggtatggtag aggtaccaga tgagcgtctg caaaaattga
     1801 cagaattgat tacgcctaaa aaaacagttc cgacaacctt tgaatttact gatattgcag
     1861 gtattgttaa aggtgcttcg aagggggaag gtctaggaaa taaattttta gctaatattc
     1921 gtgaagtgga tgctattgtt catgtggtac gtgcttttga tgatgaaaat gttatgcgtg
     1981 aacaaggtcg tgaggatgcc ttcgttgatc ctatagcaga tattgatact attaatcttg
     2041 aattaatctt agctgattta gagtcaatca ataaacgtta tgcgcgtgtt gaaaaaatgg
     2101 cacgaactca aaaagataaa gaatcagtag cagagttcaa tgttttacaa aagattaaac
     2161 ctgttttgga agatgggaaa tcagctcgta ccattgagtt tacagaagag gaagcaaaag
     2221 ttgttaaagg tctcttttta ttaacaacta aacctgtttt atatgttgct aatgttgatg
     2281 aggacaaagt agctgatcct gatgatatcg actatgttaa tcaaattcgt gcatttgcag
     2341 aaacagagag tgcagaagta gttgtgattt ctgctcgagc tgaagaagaa atatctgaac
     2401 ttgatgatga agataagtta gaatttttag aagctattgg attaacagaa tctggtgttg
     2461 ataaattaac acgagctgct tatcatcttt taggccttgg aacttacttt actgctggtg
     2521 aaaaagaggt tcgcgcttgg acatttaagc gtggtataaa agctccacaa gctgcgggta
     2581 ttatccattc agattttgaa agaggtttta ttcgtgcagt aacgatgtca tatgatgacc
     2641 ttattcaata tggatctgaa aaagcagtaa aagaagctgg ccggttacgt gaagaaggaa
     2701 aagagtatat tgttcaagat ggcgatatca tggaattccg tttcaatgtc tagtaataaa
     2761 taatcaattt gttaactttt aggttaagga tattgttatt atccttaacc atttttgcgt
     2821 tttaatggag aagataatgg taaaaatgat tgttggatta ggaaatccag gaagtaaata
     2881 taatgataca aaacataata ttggatttat ggctgttgac agaattgtaa aaaaacttga
     2941 cgtcaacttt acagaagata aaaattttaa agcagagata ggtagtgatt ttatcaacgg
     3001 tgaaaaaatt tattttatca aaccaactac ttttatgaat aacagtggta ttgcggtaaa
     3061 agccctgctt acttactaca acatttctat taaggatatg attatcatat atgatgattt
     3121 agacatggaa gtcggtaaaa tacgttttcg tcaaaaagga tctgctgggg gacataatgg
     3181 tatcaagtct attattgctc atttaggtac tcaagaattt gatcgtatta aagtaggtat
     3241 aggacgtccg aatggacgta tgacagttat taatcatgtt ctagggaagt ttgataaaaa
     3301 tgatgaaatt atgatatcaa atactcttga taaggttgac aatgctgtca attattattt
     3361 acagacgaat gattttcaaa aaacaatgca gaaatacaac ggtttaaaat gaatattata
     3421 gaattattta gccaaaataa agtggtgcgg acatggcact ctggattagt aactaatagt
     3481 cgccaattag ttatggggtt ttcaggagca agtaaagcta ttgctatagc atcagcctac
     3541 gagaagttat ctaaaaaaat tatggtagtc actgccactc aaaacgattc tgataaatta
     3601 tcaagtgata tatcaagttt aattggagaa gataacgttt atcaattttt tgctgatgac
     3661 gttcctgcag cagagtttat tttctcttca ttagacaaat caatttcacg actcagtgcc
     3721 ttgaggtttc taaaagatcc agaaaaaaac ggtgttttga ttactagtat cagtggttta
     3781 aggttactat taccaaatcc ggaagttttc tcgaaaagtc aatataagtt tgaaatcggt
     3841 caagaatgtt atttagataa attgagtaag aacttagtca atttaggtta tcagaaagtt
     3901 tctcaagtat ttagtcctgg tgaatttagc caaagaggag atattttaga tatttttgag
     3961 atgacacaag aatatcctta tcgcttggaa ttcttcggag atgaaataga tggcatcagg
     4021 cagtttgata ttgacactca aaagtctctt aaacaattag agtcagtaca gatttcgcca
     4081 gcagatgata ttattttaca agatgcagat ttcgagcgtg caaagaaaaa acttgagggc
     4141 tatctagtaa cagctagtga ggaacaacgc gcctatttga gcgaggtttt atctacaacc
     4201 gagaatcatt ttaagcactc aaatattcgt cgttttctaa gtatctttta tgaaaaagaa
     4261 tggggaatat tagactatat tcctgaggga actccacttt ttgttgatga ttttcaaaaa
     4321 attgtagatc gaaatgctaa gttagattta gaaatagcta gccttctgac ggaagattta
     4381 caacaaggca agtctcattc tagtttgaac tatttttcag atccttataa gcaattaaga
     4441 caataccaac cagcaacctt cttttcaaat ttccacaaag gattaggaaa tctgaaattt
     4501 gataaacttc atcattttac tcaatatgga atgcaagaat tttttaatca atttccattg
     4561 ttggtcgatg aaatcaatcg ttataagaaa tctggagcga cagtactgtt gcaagtagat
     4621 tctcaaaaag gtttaaatct ccttcaagaa aacttaaaag aatatgggtt agatttaatc
     4681 atttcagata aaaatgatat cgttccaaag gagtcacaac ttattgttgg gcatctttca
     4741 aatggttttt attttgcaga tgagaaaatt gttctgatta ctgaacgtga gatttatcat
     4801 aggcgtgtta aaaggaaaat tagacgcagt aatattagta atgcagaacg tctaaaagat
     4861 tataatgaac tttcagttgg cgattatgtt gttcataatg ttcatggcgt aggaaaattt
     4921 ttaggtattg aaacaattga gatacaaggt attcatcgtg attatctgac tattcaatat
     4981 caaaatgctg atcggatctc tatccccgta gaacaaattg aacttctaac aaaatatgtc
     5041 tctgcagatg gcaaagagcc taaaattaat acgttaaatg atggtcgctt caaaaaagca
     5101 aaacagagag ttgcaaagca agtagaagat attgctgatg atttgttgaa actttatgca
     5161 gagcgtagtc aattacaagg atttgcattt tctcctgacg ataatatgca aaatgacttt
     5221 gataatgatt ttgcatatgt agagacagag gatcaattgc ggtcgataaa agaaattaaa
     5281 caggatatgg aaggtaatcg tccgatggat cgattattgg ttggagacgt tggttttggt
     5341 aagacagagg ttgctatgcg tgcggctttc aaagctgtca atgaccataa acaagttgct
     5401 gttttagtcc caacaacagt tctagctcag cagcattttg aaaattttaa agaacgtttt
     5461 agtaattatc ctgttacagt tgatgtactt agtcgttttc gtagcaaaaa agaacaaaca
     5521 gatacactaa aaagattatc taagggccaa gttgatatta ttattggaac acatcggctt
     5581 ctctctcaag atgttgtatt ttcggattta gggttaatag ttattgatga ggaacagcga
     5641 tttggtgtaa agcataaaga aaagttaaaa gaattaaaaa caaaagttga tgtcttaact
     5701 ttgacagcga ccccaatacc tagaacatta catatgtcaa tgcttggcat tagagactta
     5761 tcagtcattg aaacacctcc aactaatcgt tatcctgtgc agacttacgt tttggagaca
     5821 aatcctggtt tagttagaga agctattatt cgtgagatag acagaggagg acaggttttt
     5881 tatgtttaca ataaagttga cactattgaa cagaaagttt cagaacttca ggagttagta
     5941 ccagaagcta gtattggttt tgttcatggt caaatgtctg aaattcaatt agaaaataca
     6001 ttaatagact ttatcaatgg agattacgat gttttagttg caacgacaat aattgagaca
     6061 ggagtcgata tttcaaatgt taataccctc tttgttgaaa atgcagatca tatgggcttg
     6121 tcaacccttt atcaattaag aggccgtgtg ggtagaagta atcgcattgc ttatgcttac
     6181 cttatgtaca gacctgataa agttttaaca gaaatttctg agaaaagact agatgcaatc
     6241 aaagggttta cagaattggg atctggtttt aaaatagcga tgcgtgattt atctatacgt
     6301 ggtgcaggaa atattttagg agcttcacaa agtggtttta ttgattcggt tggattcgag
     6361 atgtattcac agcttctaga gcaagcgatt gccaccaaac aaggaaaatc attaattcgt
     6421 caaaaaggta atgttgaatt agccttacaa attgacgcct atttaccagc agaatatata
     6481 tcagatgagc gccaaaaaat agaaatatat aagcgtattc gtgaattaga aactagggct
     6541 gattatgaag ccttacaaga tgagttaata gatcgttttg gagaatatcc tgatcaagtt
     6601 gcataccttt tagaaatagg acttttaaaa gcttatctgg atcttgcctt tactgaatta
     6661 gtagaaagaa aaggtaatga aataagcatc atatttgaaa aagcatcttt gaaatatttt
     6721 ttaactcaag attattttga agcactatca aaaactcaac ttaaggctag aattagcgaa
     6781 acaaatggta agatggaagt tgtttttaat atcaaacata agaaaaatta tgaaattatt
     6841 gaagaacttc taaaatttgc cgaatgtttt atcgagatta agtcacgtaa accagtagaa
     6901 gaataactaa gtgaaaagtt gcaaggaaat ttggatactc ttttagaaaa tgtttggttc
     6961 aagaagaata gtacgagaaa cattatgttt cacgtcaaac atctcaactt ttgaaagaca
     7021 taaatatttt tagaggttat taaaatagaa gttttttact tgatatttga gagaattaag
     7081 agagctaata gctctctttt tgttacatta tattgacaaa tattatcagt tcagatattt
     7141 ttcaaaaaaa atgctaaaat agtagagtta atttagatag aaggatgtga tcatcagtga
     7201 gattagacaa gtatctaaaa gtgtcgcgta tcattaagcg tcgtcccgtt gctaaagaag
     7261 ttgcagacaa aggacgtgtc aaggtgaatg gcgtattagc aaagtcatca actgacctaa
     7321 agcttaatga ccaagttgaa attcgttttg gtaataaatt gttgaccgtt aaagtattag
     7381 aaatgaaaga tagcactaag aaagaagatg ctatcaaaat gtatgaaatt attaatgaaa
     7441 caaggataga aacagatgag caagcctaat gttgttcagt taaataatca atatattaac
     7501 gatgagaatc taaaaaaacg ttacgaagct gaggagttac gccgaaaaaa tcgtttaatg
     7561 ggttgggttc ttatttttgt catgctttta tttattttac ccacttataa tttagttaag
     7621 agttacagaa ctttacaaga acgtcgtcaa gaagttgtaa aattaacgaa agactatcag
     7681 acattaacta atagaactga gaaccagaag ttactagcaa aacaactaaa aaatccagat
     7741 tacgttcaaa aatatgctcg agcgaagtat tatttctcta agaccggcga aatgatttac
     7801 ccattaccag accttttacc aaaataatta tgaataattt aattgaaaca atagaacatt
     7861 ttctagccta ctcggatgac aaactagaag agttagctga taagaactat caacttaggc
     7921 aaacaattgt agaaaaagaa aagattgaga gcagagaaaa gtaatgaaga aagttttaac
     7981 cttcttatta tgtagcttat attttgtatc aatcccagct ataagtaccg aggagccatt
     8041 aacgctttct caaaatagac gatacgcctt aactcagaca gttgtggata aggaaatgta
     8101 ttttgatgct attccagaga gaccaactac aaaaattgaa ataagcagtt ttcaagatga
     8161 agctttaacc ataacaggag agactttagt tccaaatact ttattgtcga ttatttccct
     8221 tacaattaat tcaaatggaa ttccagtatt tactttgtcc aatggacagt tcataaaggc
     8281 tagtcgtgaa gctattttta atgatttagt ttctaagcag caatctgtct cattggatta
     8341 ttggttaaaa ccttcttttg tgacttatga agcaccttac acaaatggtg ttagtgaagt
     8401 taaaaataat ttaaaacctt attccagagt gcatttagta gaacaagcag aaacagaaca
     8461 tggtatttat tataaaacgg acagtggttt ttggatatca gtagaagatc tatcggttgc
     8521 tgataatcgt atggctaagg ttcaagaagt att
//
LOCUS       DATROQ010000076         8539 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.077, whole genome shotgun sequence.
ACCESSION   DATROQ010000076 DATROQ010000000
VERSION     DATROQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8539
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.077"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..406)
                     /locus_tag="VBC94_001595"
     CDS             complement(<1..406)
                     /locus_tag="VBC94_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEO6979853.1"
                     /translation="MITYDFTSLPERFSSNAIKWKAVQKDQEILPLWIADMDFPIFPE
                     MSEAIEDFSHQMVFGYDSPKDSLYQAISNWEVQEHGYQFDKKSLLLIDGVVPAISVAI
                     QAFTKEGDAVLINTPVYPPFARTIKYNNRHLVS"
     gene            complement(542..1090)
                     /locus_tag="VBC94_001596"
     CDS             complement(542..1090)
                     /locus_tag="VBC94_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEO6979854.1"
                     /translation="MNIETLTQKNHRSDSGRNHIVLFEPQIPANTGNIARTCAATNAP
                     LHIIRPMGFPIDDKKMKRAGLDYWDKLDVSFYDGLEEFMLSCRGKVHLISKFADKVYS
                     DENYNDDQDHYFMFGREDKGLPETFMREHAEKALRIPMNDEHVRSLNVSNTVCMIVYE
                     ALRQQSFPNLELSHTYENDKLK"
     gene            complement(1263..2615)
                     /locus_tag="VBC94_001597"
     CDS             complement(1263..2615)
                     /locus_tag="VBC94_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEO6979855.1"
                     /translation="MENHNSIKQTYGLMTTIAMIVGVVIGSGIYFKVDDILKFTGGDV
                     FLGMVILVLGSFSIVFGSLSISELAIRTSESGGIFSYYEKYVSPALAATLGLFASFLY
                     LPTLTAIVSWVAAFYTLGESSSLESQIILAAVYILALSLMNIFAKRIAGGFQSLTTFV
                     KMIPLVLIALIGAFWSDKAPQLPQHLTAIQPSNVGWSWVSGLVPLYFAYDGWTIFVNI
                     APEVKNPKKNLPLAFVIGPALILLSYLAFFYGLTQILGASFIMTTGNDAINYAANIIF
                     GPSVGRLLSFIVILSVLGVANGLLLGTMRLPQAFAERGWIKSERMANINLKYQMSLPA
                     SLTVTAVAIFWLFVHFMVTKFNLLPGSDISEIAVVFNNTSLIILYVLVLSLYLKKDIK
                     NKFTGLVSPILAILGGLILLIGSLLSNFFTVLIFQCFCLLFCLICHYIYQKNNPKTHE
                     "
     gene            2821..4170
                     /gene="trkA"
                     /locus_tag="VBC94_001598"
     CDS             2821..4170
                     /gene="trkA"
                     /locus_tag="VBC94_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEO6979856.1"
                     /translation="MRIIVVGGGKVGTALCRSLVAEKHDVVLIEKKENVLKRVTKQHD
                     IMGIVGNGANYKILEQAEVKNCDIFIAITDRDEVNMISAVLAKKMGAKETVVRMRNPE
                     YSNPYFKDKNFLGFSSVVNPELLAAQYIANTIEFPNATSVEHFANGRVMLMEFKILEG
                     NKLCHTSMSQIRKKFGNIVICAIERDGKLIIPDGDATIQVKDKIFVTGNRIEMILFHN
                     YVKNKVVKNLMVIGAGRIAYYLLNILKNTNTHVKLVELNQEQAEYFSQEFPNVPVVHG
                     DGTAKNILLEESVTNFDAVATLTGVDEENIITSMFLESIGIPKNITKVNRTSLLEIID
                     DKQLSSIITPKRIAVDHVMHFVRGRVNAQDSNLEAMHHIANDRIETLQFEIKETSKLA
                     NRSLASLKLKQNILIAAIIRNNKTIFPTGEDVLTVGNRIVVITLLKNITRTSDMLAR"
     gene            4175..5614
                     /locus_tag="VBC94_001599"
     CDS             4175..5614
                     /locus_tag="VBC94_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO6979857.1"
                     /translation="MNKSMIRFLLSKLLLIEAALLAIPLTVGLIYREPQSVMMSIVIT
                     MIILIILGLLGSLFKPKNYHIYTKEGMLIVALCWILWSFFGALPFVISGQIPNIIDAF
                     FEVSSGFTTTGATILDDVSVLSPALLFWRSFTHLIGGMGVLVFALAIMENSKNSHLEV
                     MRAEVPGPVFGKVVSKLKKTAQILYLLYLLMFAVFAVILYFAGMPFFDSIIIAMGTAG
                     TGGFAVYNDSIAHYNSPLITNLVSIGMLIFGVNFNLYYLLLLRKIKAFFGDEELKTYL
                     RIVAIATFMIALNVIGMYDNFRQGLEHIFFEVSAIITTTGFGVTDITRWPLFSQVILL
                     FLMFIGGSAGSTAGGFKVMRSLILAKIARNQVLSTLYPNRVMSLHINKSVLDKNTQHG
                     VLKYLTIYLAIFMALVLVLTLDTNDFLVVISAAASCFNNIGPLLGSNETFSFFSPFSK
                     LLLSFAMIAGRLEIYPVLLMFIPKTWSKL"
     gene            complement(5638..5889)
                     /gene="yidD"
                     /locus_tag="VBC94_001600"
     CDS             complement(5638..5889)
                     /gene="yidD"
                     /locus_tag="VBC94_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEO6979858.1"
                     /translation="MLKSFLIFLVRFYQKNISPAFPASCRYRPTCSTYMIEAIQKHGL
                     KGVLMGIARILRCHPLAHGGNDPVPDHFSLRRNKTDISD"
     gene            complement(5889..6611)
                     /locus_tag="VBC94_001601"
     CDS             complement(5889..6611)
                     /locus_tag="VBC94_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6979859.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGMVTINGQVVNELATQVKAG
                     DLVEIEGSPIYNEEKVYYLLNKPRGVISSVSDDKGRKTVIDLLPQVKERIYPVGRLDW
                     DTTGLLILTNDGDFTDKMIHPRNEIDKVYLARVKGIATKENLRPLTRGVVIDGKKTKP
                     ARYTIIKVDHEKNRSVVELTIHEGRNHQVKKMFEQVGLLVDKLSRTQFGTLDLTGLRP
                     GEARRLNKKEISQLHNAAINKS"
     gene            complement(6601..7185)
                     /locus_tag="VBC94_001602"
     CDS             complement(6601..7185)
                     /locus_tag="VBC94_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEO6979860.1"
                     /translation="MTYLGSIEALLFVAGEDGLSLRQMAELLSLTPSALIQQLEKLAK
                     RYEEDDDSSLLLLETAQTYKLVTKDSYMTLLRDYAKAPINQSLSRASLEVLSIIAYKQ
                     PITRIEIDDIRGVNSSGAITRLIAFGLIKEAGKKEVLGHPNLYETTNYFLDYMGINQL
                     DDLIDASSIELVDEEVSLFSMDSINTEDKENNEN"
     gene            complement(7182..7889)
                     /locus_tag="VBC94_001603"
     CDS             complement(7182..7889)
                     /locus_tag="VBC94_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEO6979861.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYEVDIYDVPIVEVIEQYLAYIAT
                     LQAMRLEVAGEYMLMASQLMLIKSRNLLPKVVESNPIEDDPEMELLSQLEEYRRFKVL
                     SEELANQHQERAKYFSKPKQEVIFEDAILLHDKSVMDLFLTFSQMMSQKQKELSNSQT
                     VIEKEDYRIEDMMIVIERHFNLKKKTTLQEVFADCQTKSEMITLFLAMLELIKLHQIT
                     VEQDSNFSQVILRKEEK"
     gene            complement(7889..>8539)
                     /gene="xerD"
                     /locus_tag="VBC94_001604"
     CDS             complement(7889..>8539)
                     /gene="xerD"
                     /locus_tag="VBC94_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEO6979862.1"
                     /translation="KIIGGHVNSEKLALYQQSLSEFKLTARKRKLSAVNQFLFFLYNR
                     GTLKEFYRLQETEKITLAQTKSQIMDLSNFYQDTDYPSGRLIALLILSLGLTPAEIAN
                     LKKADFDTTFNILSIEKSQMKRILKLPEDLLPFLLESLEEDGDLVFEHNGKPYSRQWF
                     FNQLTDFLNEKNEQQLTAQLLREQFILKQKENGKTMTELSRLLGLKTPITLERYYR"
BASE COUNT         2760 a         1621 c         1356 g         2802 t
ORIGIN      
        1 ttgaaactaa atgacgatta ttgtatttta tagttctagc aaagggcgga tagactgggg
       61 tattaattag aacagcatct ccctcctttg tgaaggcttg tatagcaact gaaatcgctg
      121 gtacaacccc atcaattaat aataatgatt ttttatcaaa ttgataacca tgttcttgca
      181 cttcccaatt agagatagct tggtataaac tgtctttagg actatcatag ccaaaaacca
      241 tttggtgtga aaaatcttca atagcctccg acatttctgg aaagatagga aaatccatat
      301 ccgcaatcca aaggggaagg atttcttgat ctttttgaac cgccttccat tttatggcat
      361 tacttgaaaa acgttctggt aaacttgtga aatcatatgt aatcataatt actcctgact
      421 attctacagc taaaaatatc ccttgtcgcc ttggtgacta ttttaccatt tgtttttgat
      481 actatatact aattaacatt ctaaagataa aagtgaccat tttaaacgat cactttcttt
      541 attattttaa tttatcattt tcataagtat ggcttaattc taaattagga aagctttgct
      601 gcctaagggc ttcataaaca atcatacaaa cagtgtttga aacattcaag ctacgcacat
      661 gctcatcgtt catgggaatt cgtaaggcct tttcggcatg ctcgcgcata aaagtttctg
      721 gcaatccttt atcttcacga ccaaacataa aataatggtc ttgatcatca ttgtaatttt
      781 catctgagta aaccttgtca gcaaatttgc taattaggtg aactttccct cgacatgaca
      841 acataaattc ttccaaacca tcatagaaac taacatctag cttatcccag taatccagtc
      901 ccgctcgttt cattttttta tcatcaatag gaaatcccat cggtcgtatg atgtgcaaag
      961 gagcatttgt tgccgcacaa gttctagcaa tattaccagt atttgctgga atctgtggtt
     1021 caaataaaac aatatgattt cgtccgctat cggaacgatg atttttctgc gttagtgttt
     1081 caatattcat ctcatcatca ctttctagaa ctcattctga ctcgcaaaaa aaccacactg
     1141 ctcggagtca atctcagcaa acaatgtggt ttatggtggt ttgttaactt aaatcataac
     1201 aggtttgatt taaactaaca aatcgttgac actattatag cacttcagtc tacaaagtca
     1261 acctattcat gagtttttgg gttatttttt tgatagatat aatgacaaat caaacagaat
     1321 aataagcaaa aacattggaa aattaaaacc gtaaagaaat tacttaacaa actcccgata
     1381 aggaggatca aaccgcctaa aattgctaga atcggtgaaa ctaagccggt aaatttattt
     1441 ttaatatctt ttttgagata aaggctgagt actaaaacat aaagaatgat taaactggta
     1501 ttattaaaca ccacagcaat ttcactaata tcactacccg gcaataagtt aaattttgta
     1561 accataaaat gaacaaaaag ccaaaaaata gctacagccg tcaccgtcaa actagctggc
     1621 aatgacattt gatacttcaa atttatgtta gccatcctct cacttttgat ccatccgcgt
     1681 tccgcaaaag cttgaggtag acgcatcgtc ccaagtaaaa gtccattggc aacacctaat
     1741 acagatagaa taacaatgaa agataataaa cgcccaacac taggaccgaa aataatatta
     1801 gctgcataat taatagcatc attaccggtt gtcataataa agctagcacc taaaatttgt
     1861 gtcaaaccat agaaaaatgc caaatatgat aataagataa gcgctggacc aatgacaaag
     1921 gcaagtggta aattcttctt gggattcttg acttctggtg caatattaac aaaaattgtc
     1981 caaccatcat atgcaaaata caatggtacc agacctgaca cccaactcca tccaacattt
     2041 gacggttgaa tagctgttaa atgttgtgga agttgtggag ctttatcaga ccaaaaagca
     2101 ccaattaaag caattagaac taaaggaatc atttttacaa aggtcgttaa agattggaat
     2161 ccccctgcaa tacgcttagc aaagatattc atgagactaa gcgctaaaat ataaacagca
     2221 gctaaaataa tctgtgattc caaacttgat gattcaccta aagtataaaa tgcagctacc
     2281 catgaaacga ttgctgttaa agttggaaga tacaaaaaag acgcaaacaa gcctaaagtt
     2341 gctgctaaag ctgggctaac atatttttcg tagtaagaaa aaataccacc actttcactc
     2401 gttctaatag ctagctcaga aattgacaaa cttccaaaaa caatcgaaaa agaacctagt
     2461 actaaaatta ccatgcctaa aaagacatct ccaccagtaa atttaaggat gtcatcaact
     2521 ttaaaataaa tgcctgaacc aatcactaca cctacaatca tagcaattgt agtcattaat
     2581 ccataggtct gtttaataga attatgattc tccataatat ctccatttct actgtacgct
     2641 taaaggttaa ttataaccta atttagggct tgtcacaagt ttatctgtgt tatttttcac
     2701 cagattctga taagagaatc atattactct tatcagaaag atgaaaacgt tttttaacca
     2761 taattctcgt gcatttttac taaaaaaaag gtatcataga gtttattatt ggaggtaaaa
     2821 atgagaataa ttgttgtcgg cggaggtaaa gtcggaaccg cactttgtcg ctcacttgtt
     2881 gctgaaaagc acgatgttgt tttaattgag aaaaaagaaa acgtccttaa acgtgtcact
     2941 aaacagcatg atattatggg aattgttggg aatggagcaa actataaaat tttagaacaa
     3001 gccgaagtca aaaattgtga tatttttatc gctatcactg atcgagatga agttaatatg
     3061 atttcagcag ttcttgccaa aaaaatgggg gcaaaagaga ctgttgttcg catgcgtaat
     3121 cctgagtatt ctaatcctta cttcaaagat aaaaactttc ttggcttctc ttcagttgtc
     3181 aaccctgaat tgttagcagc acaatatatc gctaatacca tagaatttcc aaatgcaact
     3241 tcagttgaac acttcgccaa cgggcgtgtg atgttaatgg aatttaaaat tttagaagga
     3301 aataaactct gtcatacttc aatgagtcaa attcgtaaaa aatttggtaa tattgttatt
     3361 tgcgcaattg aacgcgatgg caaattgatt attcctgatg gcgatgcaac tatccaagtc
     3421 aaagataaaa tctttgtaac tggaaatcgt attgaaatga tacttttcca taattacgtt
     3481 aaaaataaag ttgtcaaaaa cttgatggtt atcggtgcag gacgcatcgc ctactaccta
     3541 ctcaatatcc ttaaaaatac caatacacac gtcaaactag ttgaactcaa ccaagagcaa
     3601 gctgaatatt ttagtcaaga atttcccaat gttcctgtcg ttcatgggga tggtactgct
     3661 aaaaatattt tgttagaaga aagcgttaca aattttgacg cagttgctac tttgactggt
     3721 gttgatgaag aaaatatcat tacctcaatg ttcttagaaa gtatcggtat ccctaaaaac
     3781 attaccaaag ttaatcgaac tagcctacta gaaatcattg acgataaaca actatcaagc
     3841 attatcacac ccaagcgtat tgccgtagac catgtgatgc actttgtacg cgggcgtgta
     3901 aatgctcagg attccaacct agaagctatg catcatattg ctaacgatcg cattgaaacc
     3961 ttacaatttg aaataaaaga aactagcaaa ttagcaaatc gtagccttgc atctttaaaa
     4021 ttaaagcaaa atattttaat tgcagcgatt attcgcaata ataaaacgat tttcccaact
     4081 ggtgaggatg ttttaacagt gggtaatcgt atcgttgtta tcaccttatt aaaaaatatt
     4141 actagaacta gtgatatgtt agcgaggtag gaatatgaat aaaagtatga ttcgtttcct
     4201 tctctcaaaa cttttgctaa ttgaagctgc tcttctagcg attcctctaa ctgttggact
     4261 tatttaccgt gaaccgcaaa gtgtcatgat gagtatagtc attaccatga ttattttaat
     4321 catccttgga ttactgggtt ctctctttaa acctaaaaat tatcatatct acactaaaga
     4381 aggcatgtta attgttgcac tctgctggat actttggtcc ttctttggcg cccttccatt
     4441 tgttatttca gggcaaatcc ctaatattat cgatgctttc tttgaagtat cttctggttt
     4501 tacgacaaca ggagcaacta ttcttgatga cgtcagtgtc ttatcacccg cgctcttatt
     4561 ttggcgtagc tttacccatc ttatcggtgg aatgggggtt ttagtatttg cccttgccat
     4621 catggaaaat tctaaaaata gccatttaga ggttatgaga gctgaagttc ctggacctgt
     4681 atttggtaaa gttgtctcga aacttaaaaa aactgcacaa attttgtatt tgttatatct
     4741 tcttatgttt gcagtatttg cagtaatatt atattttgca ggtatgcctt tctttgatag
     4801 tattatcata gcgatgggaa ctgcaggtac aggtggtttt gcagtttaca atgatagcat
     4861 tgctcattat aatagtcctc tgataactaa cttagtttct attggtatgc ttatcttcgg
     4921 ggttaacttt aatctttact atcttctttt actccgaaaa attaaagcat tctttggtga
     4981 tgaggaacta aaaacctatc tccgcatcgt ggcaatagca actttcatga ttgctctaaa
     5041 cgtcatcggt atgtatgata attttcgaca aggcctcgaa catattttct ttgaagtatc
     5101 agctattatt acgacaactg gttttggcgt gactgatatc actcgttggc ctttgttttc
     5161 acaggttatt ttgctattct tgatgtttat cggtggttct gctggctcta cagctggggg
     5221 ctttaaagtc atgcgtagct taatcctagc aaagattgca cgtaaccaag tattgtctac
     5281 actctatcct aatagagtca tgtcactaca cattaataaa agcgttctag ataaaaatac
     5341 acaacatggt gttttaaaat atctaactat ctatttagct atcttcatgg cactcgttct
     5401 tgttttaacg cttgatacta atgatttttt ggttgttatc agtgctgcag cctcatgttt
     5461 taacaacata ggcccactct taggtagcaa tgaaactttc tcattcttta gtccattctc
     5521 aaaactcctt ctttcatttg ctatgatagc aggacgttta gaaatatacc ctgttttatt
     5581 gatgtttatt cctaaaacat ggtcaaaact ataattacaa ataataaaac ggatatatca
     5641 atctgatata tccgttttat tacgtcttaa gctaaaatga tcagggacag gatcatttcc
     5701 tccgtgggct aagggatgac atcgcaaaat acgtgcaatc cccatcaaca caccttttag
     5761 accatgtttt tgaatagctt ctatcatata cgtagagcaa gttggacgat aacgacagct
     5821 agctgggaaa gctggagaaa tatttttttg gtaaaagcga actaagaaaa tcaaaaaaga
     5881 tttaagcatt aagatttatt aatagccgca ttatgcaact gactaatttc ttttttattt
     5941 aatcgtcgtg cttccccagg acggagaccc gttaaatcta aggtaccgaa ttgtgtacga
     6001 gatagcttgt ccactaataa cccaacttgt tcaaacattt tcttaacttg atgattacga
     6061 ccttcatgaa tagttaattc aactactgaa cgattttttt catgatccac ttttatgata
     6121 gtatagcgag caggttttgt tttcttacca tcgataacaa cgccacgcgt taatggtcga
     6181 aggttttctt tagtagctat tcctttaaca cgtgctaaat aaactttatc aatttcatta
     6241 cgaggatgaa tcattttatc agtaaaatca ccatcattag tcaaaattaa aagtccagtt
     6301 gtatcccaat ccaagcgacc aacaggatat atccgttctt tgacttgtgg caacaagtca
     6361 ataactgtct tacgtccttt atcatctgag acacttgaaa taacaccacg tggtttattt
     6421 aagagataat ataccttctc ttcgttgtaa attggacttc cttcaatttc aaccagatca
     6481 ccagccttta cttgggtcgc caattcatta accacttgac cattaatcgt gaccattcct
     6541 tgcttgatta attcctcagc ttttctacga cttgcaatcc ctgcatgcgc aatatattta
     6601 ttaattctca ttgttttcct tatcttctgt attaattgaa tccattgaaa ataacgatac
     6661 ttcttcgtca actaattcaa ttgaagaggc gtcaattaag tcatctaact gattaattcc
     6721 catataatct aagaaataat tagttgtctc ataaaggtta ggatgtccca acacttcttt
     6781 ctttccagcc tctttaatta aaccaaaagc tatcagtctc gttatagctc cactggaatt
     6841 cacgccacga atatcatcaa tttcaatcct agtaataggt tgtttataag caataataga
     6901 tagaacttcc aaacttgccc gagatagact ctgattaatc ggagccttcg cataatctct
     6961 caacaaagtc atataactat cctttgttac caatttataa gtttgtgctg tctcaagcaa
     7021 taatagacta gaatcgtcat cttcttcata tcgtttagcc aacttttcta gttgctgtat
     7081 taatgctgat ggtgtcaaag acaataattc tgccatttga cgcaaactca gtccatcttc
     7141 acctgcaaca aaaagaagag cttctattga gcctaaataa gtcatttctc ttcctttctt
     7201 aagataactt gactaaaatt actgtcttgt tctactgtga tttgatgtaa ttttatcaac
     7261 tctaacattg ccaaaaataa agttatcatt tcagattttg tttggcaatc cgcaaacact
     7321 tcttgtaatg tcgttttctt tttaagatta aaatgtcttt caataacgat catcatatct
     7381 tcaatacggt agtcttcttt ctcaattacc gtttgactat ttgacaattc tttttgtttt
     7441 tgggacatca tttgagaaaa agtcaaaaaa agatccatca ccgatttatc gtgtagaaga
     7501 attgcatctt caaagataac ttcttgtttt ggttttgaaa aatatttggc gcgttcctga
     7561 tgctgattag ctaactcttc acttagcact ttaaaacgtc tatattcctc taattggctc
     7621 aacaattcca tttctggatc atcttcaata ggatttgact ccactacttt aggtaataag
     7681 ttacggcttt ttatcaacat cagctgactt gccataagca tatactcacc agctacttct
     7741 agacgcatcg cctgtaatgt tgctatataa gcaaggtact gctcaataac ttccacaatc
     7801 ggtacatcgt atatatcaac ctcatattta gatacgaggt gcaagagtaa gtctaggggc
     7861 ccctcgaaat cttttaactt aatatccatt atctataata tctctctaaa gtgataggag
     7921 tttttaagcc tagaaggcga ctgagctccg tcatcgtttt gccattttct ttttgcttta
     7981 gaataaattg ttctcgcaga agttgtgcag ttagttgttg ctcattcttc tcatttaaga
     8041 aatctgttaa ttgattaaaa aaccattgtc tagaataagg cttaccgtta tgctcaaaga
     8101 ctaagtcacc atcttcctct aaagattcca aaagaaaagg aagcaagtct tctggaagtt
     8161 ttaaaatacg cttcatctgc gatttttcaa tacttaagat attaaaagtt gtatcaaaat
     8221 cagccttctt caaattagca atttcagccg gagttaaacc taaagataaa atcaacaaag
     8281 caatcaaacg accactcgga taatcagtat cttgatagaa atttgataaa tccattattt
     8341 gagactttgt ctgagccaaa gtgatttttt cagtctcttg tagtctgtaa aattccttca
     8401 gcgttcctct attatataaa aagaaaagaa attgattgac tgcagataac tttcgcttac
     8461 gagctgttaa cttaaattca cttagagact gttgatacaa tgccagtttt tcagaattta
     8521 catgtccacc aattatttt
//
LOCUS       DATROQ010000077         8531 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.078, whole genome shotgun sequence.
ACCESSION   DATROQ010000077 DATROQ010000000
VERSION     DATROQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8531
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.078"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            64..180
                     /locus_tag="VBC94_001605"
     CDS             64..180
                     /locus_tag="VBC94_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEO6979863.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKHYGKK"
     gene            complement(272..1156)
                     /locus_tag="VBC94_001606"
     CDS             complement(272..1156)
                     /locus_tag="VBC94_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEO6979864.1"
                     /translation="MSLIILTVILVLVIVLLITSLYVVKQQTVAIIERFGKYQKTATS
                     GIHIRVPLGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNIATQYRVNENNVTDAYY
                     KLIKPEAQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIVKT
                     LITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVGIA
                     QQRKAIVDGLADSIQELKDANVTLTEEQIMSILLTNQYLDTLNTFAINGNQTIFLPNN
                     PEGVEDIRTQVLSALKTR"
     gene            complement(1304..2968)
                     /locus_tag="VBC94_001607"
     CDS             complement(1304..2968)
                     /locus_tag="VBC94_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEO6979865.1"
                     /translation="MSKITTSLFQEMVQAASTRLSKQAEYVNSLNVFPVPDGDTGTNM
                     GMTMENGAKEVSDKPATTVGEVGQILSKGVLMGARGNSGVITSQLFRGFGQSIKDKEE
                     LTGQDLAHAFQNGVEVAYKAVMKPVEGTILTVSRGAATAALKKAEETDDAVEVMRATL
                     KGAKRALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFKATPATM
                     TEMVNAEHHKAVVGHVATEDIKYGYCTEVMVGLKQGPTYVKEFNYEEFQGYLSNLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVENMRNQHDAQMQKVEVEETVK
                     ETKEYGIIAVVAGDGLAEIFKSQGVDYIISGGQTMNPSTEDIVKAIEKVNARNVIILP
                     NNKNIFMAAQSAADVVDIPAAVVETRTVPQGFTSLLAFDPAKSLETNVADMTNSLSDV
                     ISGSVTLAVRDTTIDGLEIHENDILGMVDGKILVSTPDMEKALKDTFDKMIDEDSEIV
                     TIYVGEDGKQALAETLSEYLEETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            complement(2968..3333)
                     /locus_tag="VBC94_001608"
     CDS             complement(2968..3333)
                     /locus_tag="VBC94_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6979866.1"
                     /translation="MTVKINTRDGQIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQALLRKENYAKGVVVRSSEAGIAVDVYTVMSYGVKISEVSKNIQERVKFNLENQLG
                     LSADMVNVYVQSIKVVGED"
     gene            complement(3465..3653)
                     /locus_tag="VBC94_001609"
     CDS             complement(3465..3653)
                     /locus_tag="VBC94_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEO6979867.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTVLIDGKP
                     KKVWVSARALKSGKVERV"
     gene            3892..4809
                     /locus_tag="VBC94_001610"
     CDS             3892..4809
                     /locus_tag="VBC94_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO6979868.1"
                     /translation="MTRKIGIIGMGHVGSTVAHALIAQGIGDDYVFIDTNVDKVNADM
                     IDFQDALANATYHANIVLNDYDALSDADVVISALGDISLQNNKEANRFAELPFTSKEV
                     YDVSQKLKSSGFRGILIVVTNPVDAITQLYQEYTQFPKSRVIGTGTLLDTARMKRVLG
                     QRLQVDPRSIGGYNLGEHGNSQFVAWSQVTVKGQPAISLLKVEELEQIKEASLKGGHT
                     VFFGKKYTSYGIATAAVRIVLAVLNDSHELLPVSTFYESEGVYLGYPAIIGRVGIVER
                     ISLSLSPKEEQQLTDSAAFIRNYLKHINL"
     gene            complement(4894..5775)
                     /locus_tag="VBC94_001611"
     CDS             complement(4894..5775)
                     /locus_tag="VBC94_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEO6979869.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKLCKQLIETLVESMGITVPVAIHLDHGHYDDALECIEVGYTS
                     IMFDGSHLPVEENLEKAREVVAKAHAKGISVEAEVGTIGGEEDGIVGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPANWEGLDLDHLKKLTEAVPGFPIVLHGGSGIPDDQI
                     QEAIKLGVAKVNVNTECQLAFCQATRDYAVEFNANEAEYMKKKLFDPRKFLKPGFDAI
                     TEAVEERIDVFGSANKA"
     gene            complement(5915..7303)
                     /gene="argH"
                     /locus_tag="VBC94_001612"
     CDS             complement(5915..7303)
                     /gene="argH"
                     /locus_tag="VBC94_001612"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEO6979870.1"
                     /translation="MTKNHKLWGGRFESSLEKWVEEFGASISFDQKLAPYDMKASMAH
                     VTMLGKTDIISQEEAGLIKDGLKILQDKYRAGQLTFSISNEDIHMNIESLLTAEIGEV
                     AGKLHTARSRNDQVATDMHLYLKDKLQEMMKKLLHLRTTLVNLAENHIYTVMPGYTHL
                     QHAQPISFGHHLMAYYNMFTRDTERLEFNMKHTNLSPLGAAALAGTTFPIDRHMTTRL
                     LDFEKPYSNSLDAVSDRDFIIEFLSNASILMMHLSRFCEEIINWCSYEYQFITLSDTF
                     STGSSIMPQKKNPDMAELIRGKTGRVYGNLFSLLTVMKSLPLAYNKDLQEDKEGMFDS
                     VETVSIAIEIMANMLEKMTVNEHIMMTSTETDFSNATELADYLASKGVPFRKAHEIVG
                     KLVLECSKNGSYLQDIPLKYYQEISELIENDIYEILTAKTAVKRRNSLGGTGFDQVKE
                     QILLARKELKAE"
     gene            complement(7322..8512)
                     /locus_tag="VBC94_001613"
     CDS             complement(7322..8512)
                     /locus_tag="VBC94_001613"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEO6979871.1"
                     /translation="MGKEKLILAYSGGLDTSVAIAWLKKDYDVIAVCMDVGEGKDLDF
                     IHDKALTIGAIESYILDVKDEFAEHFVLPALQAHAMYEQKYPLVSALSRPIIAQKLVE
                     MAHQTGATTIAHGCTGKGNDQVRFEVAIAALDPELKVIAPVREWQWHREEEITFAKAN
                     GVPIPADLDNPYSIDQNLWGRANECGVLENPWNQAPEEAFGITKSPEEAPDCAEYIDI
                     TFQNGKPIAINNQEMTLADLILSLNEIAGKHGIGRIDHVENRLVGIKSREIYECPAAM
                     VLLAAHKEIEDLTLVREVSHFKPILENELSNLIYNALWFSPATKAIIAYVKETQKVVN
                     GTTKVKLYKGSAKVVARHSSNSLYDENLATYTAADNFDQDAAVGFIKLWGLPTQVNAQ
                     VNKG"
BASE COUNT         2557 a         1724 c         1373 g         2877 t
ORIGIN      
        1 gttaattttt ttgtggtata ctggtgtctg ttaacgttaa taaattgtct ataggaggta
       61 atcatggctg aaaaaacaga cctatcgtca gcctatcgca gacttaaaag tcctaacatt
      121 aaaactagaa aacgtgctct gaaaatcatt catgagtaca aacattacgg taaaaaataa
      181 cttttttccg tctctccaat tataataaat aagtcagaca caaaataacc agagcaaatg
      241 ctctggttat tttgcttcta taaaaagttc attatcttgt tttaagagca gataaaactt
      301 gtgtacgaat atcttcaaca ccttctgggt tatttggtaa gaaaatcgtt tgattaccat
      361 taatagcaaa tgtatttaaa gtatctaaat attgatttgt taacaagata gacatgattt
      421 gttcttctgt caatgtcacg ttagcatctt tcaattcttg aatcgaatca gctaacccat
      481 ctacaattgc tttacgttgt tgcgcaatac ccacaccatg tagacgatct ttttctgctt
      541 ctgcttctgc agcagttaca attttgatct tatcagcatt cgctaattct tgagctgcta
      601 cacgtttacg ttgtgcagca ttgatttcgt tcatggattg ttttacttct gcatctggct
      661 caaccttagt aattaaagtc ttaacaatga tgtagccgta tgttgacatt tcttctgcca
      721 cttgatgttg aacttccaag gctatttcat ctttcttttc gaatagctca tctaatgtaa
      781 gtttaggaac tgatgaacgc aaagcatctt cgatataaga tttaatctga gcttctggct
      841 ttattaattt gtaataagcg tctgtaacgt tattttcatt gacacgatat tgtgtagcaa
      901 tatttaaagt gacaaaaaca ttatcttttg tttttgtctc aactataatt tcactctgta
      961 aaagacgtaa ctgcactcgt gctgcaatct tatcgatacc caatggtaca cgaatatgaa
     1021 taccgctagt agcagtcttt tgatatttac caaagcgttc aatgatggct accgtctgtt
     1081 gtttaacaac gtataaagaa gttatcaaca gaacaatcac taaaaccaaa ataaccgtaa
     1141 gaataattaa tgacatcata aatctccttt gtatgaatgt tttaaaacaa ttctagcaca
     1201 atttgtcaaa attttcaaaa tactgatgct ttatcatact aaaatagaaa ctctgataaa
     1261 ataaaaaatt agaagtatct ctcctaattt tttattttat caattattca acgctcatga
     1321 ggtatggata tacaggttga tctccttggt ggatctcaac ttcaacatct tcataagtct
     1381 cctcaagata ttccgacaag gtctcggcta atgcttgttt accatcttca ccaacataga
     1441 ttgtcacgat ttcactatct tcatcaatca ttttatcaaa agtgtcttta agtgcttttt
     1501 ccatatctgg tgtagatact agaattttac catcaaccat cccaaggatg tcgttttcat
     1561 gaatttcaag gccatctatc gttgtatcac gaacagctaa agtcacacta ccactaatga
     1621 catctgagag gctatttgtc atatcagcta cattagtttc aagagactta gcaggatcaa
     1681 aagcaagtaa gctagtaaat ccttgtggaa ctgtacgtgt ctctactact gctgctggaa
     1741 tatcaacgac atctgcagct gactgtgcag ccatgaagat gttcttattg tttggtaaaa
     1801 taataacatt tctagcgttt actttttcaa tcgcttttac aatatcttct gtagatgggt
     1861 tcatagtctg gccgccagag atgatataat caacaccttg tgatttaaaa atctcagcta
     1921 gaccgtcacc agctacaaca gcaataatac catattcttt agtttctttg acagtttctt
     1981 caacttcaac cttttgcatt tgggcatcat gttggttacg catgttttca actttaacct
     2041 tcacaagcga tccatatttc aaaccttctt gcataacaag acctggatct tctgtatgaa
     2101 catgaacctt gacaatttca tcatcattaa caactagtaa agaatcccct agattgctaa
     2161 gataaccttg aaattcttcg tagttaaatt ccttaacgta agttggtcct tgctttaaac
     2221 caaccatgac ttctgtacaa tagccatatt taatatcttc tgtggctacg tggcctacta
     2281 cagctttatg gtgctctgca ttaaccattt cagtcattgt tgcgggagtt gctttaaaat
     2341 cttctgatgc gatatattct ccagtcagtg ctgagaggaa accttcgtaa ataaagacga
     2401 gtccttgacc accagaatca acaacaccaa cctctttaag aacaggtagc atatccggtg
     2461 ttttagcaag agcacgctta gcacctttga gagttgcgcg cataacttca actgcatcat
     2521 cagtttcttc agcttttttt agagcagcgg tagctgcacc gcgtgatact gtcaagatag
     2581 ttccttcaac tggtttcata acggctttat aagctacttc aacaccattc tggaaagcgt
     2641 gtgccaaatc ttgacccgtt aattcttctt tatccttgat actttgacca aaaccacgga
     2701 ataattgtga tgtaattaca cctgaatttc cacgtgctcc catcaataca cctttagaaa
     2761 gaatttgacc aacttctcca acagtcgtcg ctggtttgtc cgaaacttcc ttggctccgt
     2821 tttccatagt catccccata ttcgtaccag tatcaccatc tggaactggg aatacattta
     2881 atgagttgac atactctgcc tgtttactca gacgtgtact ggcagcttgt accatttctt
     2941 ggaataaact tgtcgtaatc tttgacacta atcttctcct acaaccttaa tactttgcac
     3001 ataaacattc accatatcag cagataaacc aagctgattt tcgagattga atttcactcg
     3061 ctcttgaata ttttttgaaa cttcactaat tttgacgccg taactcatga cagtataaac
     3121 atctactgcg atgcccgctt cactagacct cacaacaaca ccttttgcgt agttttcctt
     3181 acgcaaaagt gcttgaaagt tatctttgat agcactttta cttgccatac caacaacgcc
     3241 aaaaatttcc gttgctgaac ctccaacgac agttgcaata acgtcatcag agagttcaat
     3301 ttgaccatct cttgtattga tttttacagt catatctact acctcaaaaa gtttttatca
     3361 ctctattcta tcatatctca ctattattgt aaaaaaatag aggtatctaa ctatcaaatc
     3421 agccttagaa acactaaaaa aagccctaag gctttctcag tttattaaac gcgttctact
     3481 ttaccagatt taagcgcacg agctgaaacc caaacttttt ttggtttacc gtcgataaga
     3541 acagtaactt tttgaaggtt tggtttaaca gtacgttttg tttggttcat cgcgtgtgaa
     3601 cggttgttac cagaaactgt tttacgtcct gtgaaataac atactttagc cattttacga
     3661 gttcctccta tattgatcaa tatttacgga tgtgctagca ccacatacta gattattcta
     3721 tcacaactta cttcttctag caagttcttt tcaaaattac tttttcattt gttttatcta
     3781 aatcgcttac atatttttta taataagaca cacttccttc taattcattt tttagtttac
     3841 attttctttc aaaatattat aatagacaac aataatcaaa ggaggtttca tatgacacgt
     3901 aaaattggaa ttataggcat ggggcacgtt ggatcaactg ttgcacacgc tcttattgca
     3961 caaggtattg gtgatgatta tgtctttatt gatactaatg ttgataaggt taatgctgat
     4021 atgattgatt ttcaagatgc tctggcaaat gctacttatc acgctaatat agtattaaac
     4081 gactatgacg cgttgtcaga tgctgatgtt gtgatttcag ccttaggtga tatttcacta
     4141 cagaataaca aagaagctaa tcgctttgct gaactaccat ttaccagtaa agaagtctac
     4201 gacgttagcc aaaagttaaa atcatctggt tttagaggga tccttattgt cgttactaat
     4261 cctgttgatg ccatcaccca gctttatcaa gaatatactc aatttcctaa atcacgcgtt
     4321 atcggaacag gaaccttatt ggatactgct cggatgaaaa gagtgcttgg tcaacgccta
     4381 caagttgatc caaggagtat cggtggttat aatctaggag agcatggtaa ctcccaattt
     4441 gtcgcttgga gtcaggtaac tgtaaaagga caaccagcta tatcactatt aaaagtagaa
     4501 gaattagaac agataaaaga agcttcttta aaaggcgggc acactgtctt ttttggtaaa
     4561 aaatatacta gttatggtat cgctaccgca gctgtccgca tcgttttagc tgtgttaaat
     4621 gattcccatg agttactacc tgtatctacc ttctatgagt ccgaaggggt ttaccttgga
     4681 tatccagcta ttattggtag agtaggaatt gtagaaagaa tttctctttc actaagccca
     4741 aaagaagagc aacaactcac agattcagcg gcatttattc gaaattatct taagcatatc
     4801 aatctttaaa gagtatctat ttaataaaaa gtcatataat ccaaaaaaag ctcaccataa
     4861 gtgagctttt aactattatc tgttttatgt taattatgct ttgtttgctg aaccaaacac
     4921 gtcaatacgt tcttcaactg cttctgtaat tgcatcgaaa ccaggtttca agaatttacg
     4981 tgggtcaaag agttttttct tcatgtattc agcttcgtta gcgttgaatt caacagcgta
     5041 gtcacgagtt gcttgacaga aagctaattg acattctgtg ttaacgttaa ctttagcaac
     5101 accaagtttg atagcttctt ggatttgatc atcaggaata cctgaaccac cgtgtaacac
     5161 gattgggaaa ccaggaacag cttcagtcaa tttcttcaag tgatcaaggt caagtccttc
     5221 ccagtttgct gggtatggtc catgaatgtt accgatacct gcagccaaga agtcaatacc
     5281 agtttcaacc atagctttag cgtcttcgat tggtgctaat tcacctttac cgacaatacc
     5341 atcttcttca ccaccgatag taccaacttc agcttctact gaaatacctt tagcatgtgc
     5401 tttagcaaca acttcgcgtg ctttttcaag gttttcttca actggaaggt gtgaaccatc
     5461 aaacataatt gaagtgtaac ctacttcaat acactcaagg gcatcatcgt aatgaccgtg
     5521 gtcaaggtgg atagcaactg gtacagtaat acccattgat tcaaccaaag tttcaataag
     5581 ttgtttacaa agtttgtaac cacccatgta tttagccgca cccattgaag tttggataag
     5641 tactggagct tttttagctt ctgctgcgcg caagatagct tgagtccact caaggttgtt
     5701 tgtgttaaat ccacctactg catagccgtt atcacgagct gcttggacga atttttctgc
     5761 tgaaacgatt gccattattc aggcctccta tttatttttt gggcgttgcc cacttacatg
     5821 ttaattctag cacaattcta taaaaaatgc tagtttttta ctgaaaatac gtgagaaact
     5881 taacagagtt aaacgaggtt ttttcagaaa gcagtcattc cgctttcaat tctttacgtg
     5941 ccaacaggat ctgttcttta acctggtcaa accctgttcc tcctaatgaa tttcttcgtt
     6001 taactgctgt ctttgcagtt aatatctcat agatatcgtt ttctatcaac tctgaaattt
     6061 cttgataata ttttaaaggt atatcctgta aataagaacc atttttactg cattctaaaa
     6121 ccaatttacc tacaatttca tgtgcttttc tgaaaggtac ccccttactt gctaaataat
     6181 ctgctaattc tgttgcattt gaaaaatcag tctccgttga ggtcatcatt atgtgctcat
     6241 tcacagtcat tttctcaagc atattagcca tgatttcaat agctatgctg actgtttcta
     6301 cgctatcaaa catcccttct ttatcttctt gtaaatcttt gttataagct aacggcaatg
     6361 atttcataac agttagtaaa ctaaataaat ttccataaac acggcctgtc ttccctcgaa
     6421 ttaattctgc catatcagga tttttctttt gaggcataat agatgatcct gtagagaaag
     6481 tatctgataa tgtgatgaat tgatattcat aactgcacca attaatgatt tcttcacaaa
     6541 aacgtgataa atgcatcatt aaaatgctag cattggataa aaattctatg ataaagtcac
     6601 gatctgagac agcatcaagc gaattactat atggtttttc aaaatctaat aaacgagttg
     6661 tcatatggcg gtcaatagga aatgttgtcc ctgctagagc tgctgctcct aatggtgata
     6721 gattggtgtg tttcatatta aactctaatc gctctgtatc acgcgtaaac atattatagt
     6781 aagccattag atgatgtcca aatgaaatcg gctgagcatg ttgtaaatgt gtatatcctg
     6841 gcataacagt atagatatga ttttctgcta aattaactaa ggttgttcgt aaatgaagca
     6901 atttcttcat catttcttgc aatttatctt tcaaataaag gtgcatatct gtagccactt
     6961 gatcatttcg tgaacgtgca gtatgaagct tacctgctac ttctccaatt tcagcggtta
     7021 ataagctctc tatgttcata tgaatgtctt catttgatat gctaaaagtc aattgacctg
     7081 ctctgtattt atcttgcagt atttttaaac catcttttat caagcctgct tcttcttgac
     7141 taataatatc cgttttgcct aacattgtga catgggccat agaagccttc atatcatagg
     7201 gagctaactt ttggtcgaaa ctgattgatg caccgaactc ttcaacccat ttttcaagac
     7261 tactttcaaa acgaccaccc cataacttat gattttttgt catcctctta tcttccttta
     7321 tttatccttt gtttacttgt gcattaactt gggttggaag tccccagagt ttgataaaac
     7381 caactgctgc atcttgatca aagttatcag cagctgtata agttgctaag ttctcatcat
     7441 ataatgaatt agacgagtgg cgagcaacga ctttggcaga acctttatat aacttaactt
     7501 ttgtagtacc attaaccact ttttgtgttt ctttgacata ggcaatgata gcttttgtag
     7561 ctggactaaa ccataaagca ttataaataa gatttgatag ttcattttca aggataggtt
     7621 taaaatgaga cacttcacgg actaatgtta aatcttcaat ttctttatga gcagctaaca
     7681 gtaccattgc cgcaggacat tcataaattt cacgtgattt gataccgact aaacgatttt
     7741 caacatgatc aatgcgtcca ataccatgtt ttcctgcaat ttcatttagc gataaaatca
     7801 aatctgctag tgtcatttct tgattgttta tagctatagg cttcccattt tggaacgtaa
     7861 tatcaatata ttctgcacaa tcaggagctt cttctggtga ttttgtaata ccaaaagctt
     7921 cttctggcgc ttgattccaa ggattctcaa gcacaccaca ttcattggca cgtccccata
     7981 aattttggtc tatagaataa gggttatcta aatctgctgg aataggtacg ccgttagctt
     8041 ttgcaaaagt aatttcctct tcacgatgcc actgccactc acgaacaggg gcaattactt
     8101 ttaattcagg atctagtgct gcaattgcaa cttcaaaacg cacttggtca tttcctttac
     8161 ctgtacatcc atgtgcaatt gtggtcgcac ctgtttgatg tgccatttca actaattttt
     8221 gggcaataat tggacgacta agcgctgaaa caagcgggta tttttgttca tacatcgcat
     8281 gtgcctgtaa agctggtaaa acaaaatgct cagcgaactc atctttaaca tctaaaatat
     8341 aagattctat ggcacctatt gttaaagctt tatcgtgaat aaaatcaaga tctttacctt
     8401 cgccaacatc catacagaca gcaataacgt cataatcttt ttttaaccaa gctatagcaa
     8461 cagaggtatc taaaccacca gaataagcta aaattaattt ttcttttccc ataaaaaatc
     8521 cccttccaaa t
//
LOCUS       DATROQ010000078         8440 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.079, whole genome shotgun sequence.
ACCESSION   DATROQ010000078 DATROQ010000000
VERSION     DATROQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.079"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            87..2720
                     /locus_tag="VBC94_001614"
     CDS             87..2720
                     /locus_tag="VBC94_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6979872.1"
                     /translation="MKGQKIIALAGLVLSCHFALTACHTQEHKNSYHIKTKQVAKKKA
                     NKKKVSVKESHKKRKGVAGVDFPTDDGFLLTKDSKILSHTDSGIVVAHGNHSHFIFYS
                     DLKGSKFSYLIPNANAKTNKNQAVRNFKAGAVAVNTLNDGYVFNPADIVSEDANGYVV
                     RHGDHFHYIPKASLSQQKQVQASRAVSRLGNQSNSHYRVNSSKIAGLHYPTSDGFLFN
                     GRGIKGTTPTGILVEHHNHLHFISFADLRKGGWGSIADRYQPQKKADSKKQSPSSKKP
                     RNENTLPKDIKDKLAYLARELHLDISRIRVLKTLNGEIGFEYPHDDHTHVIMAKDIDL
                     SKPIPNPHHDDEDHHKGHHHDESDHKHEEHEHTKSNKLSDENQKKLIYLAEKLGLNPN
                     QIEVLTSEDGSIIFKYPHDDHSHTIASKDIEIGKPIPDGHHDHSHAKDKVGMATLKQI
                     GFDDEIIQDILHADAPTPFPSNETNPEKMRQWLATVTKINIGQRTNPFQRFGLSLMPN
                     IEVLGIGFTPINDMTPVLQFKKLKQLWMTNTGITDYSFLDKMPLLEGLDISQNGIKDL
                     SFLTKYKQLSLIAAANNGITSLKPLAELPNLQFLVLSHNNISDLTPLSNLTKLQELHL
                     DHNNVKNLSALSGKKDLKVLDLSNNKSADLSTLKTTSLETLLLNETNTSNLSFLKQNP
                     KVSNLTINNAKLSSLDGIEESDEIVKVEAEGNQIKSLVLKNKQGSLKFLNVTNNQLTS
                     LEGVNNYTSLETLSVSRNKLKSLDIKTPNKTVTNLDFSHNNVPTSQLKLNEKNIPEAV
                     AKNFPAVVEGSMVGNGSLAEKAAMASKEDKQVSDNTNHQKNTEKSAQANADSKKENPK
                     THDEHHDHEETDHAHVGHHHH"
     gene            2820..3479
                     /locus_tag="VBC94_001615"
     CDS             2820..3479
                     /locus_tag="VBC94_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6979873.1"
                     /translation="MTMVKREDVILNATENMVYHKLKNDPSGHDWFHIVRVRNLAVEL
                     AHKEGANTFICQMAALLHDIIDDKICQDSKQASYELTQWLYSQDLAIAEVEHILDILE
                     NISFKAGTGLTMKTLEGQIVQDADRLDAMGAIGIARTMAYSGSKGRLIHDPNLKPREN
                     LTLEEYRNGQDTAIMHFYEKLLKLKDLMNTKQGKMLAQKRHDFLELYLAEFYAEWNGK
                     G"
     gene            3488..3952
                     /locus_tag="VBC94_001616"
     CDS             3488..3952
                     /locus_tag="VBC94_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6979874.1"
                     /translation="MIRRAKEKDLPAIAELLKQILVLHHGVRPDIFHTRGSKFSKEQL
                     KEMLMDESRPIFVYESDEGKVVAHLFLQLQEKRDLPRKSFKTLYIDDLCIDEEVRGQQ
                     IGQKLMDFARQYAKKHGCYNITLNVWNDNQRAVSFYEKLGFKPQQTQMEEIL"
     gene            3952..4389
                     /gene="msrB"
                     /locus_tag="VBC94_001617"
     CDS             3952..4389
                     /gene="msrB"
                     /locus_tag="VBC94_001617"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEO6979875.1"
                     /translation="MKETQEELRQRIGHTAYQVTQNSATEHAFTGKYDDFFEEGIYVD
                     IVSGEVLFSSLDKFQSGCGWPAFSKPIENRMVTNHQDHSHGMHRIEVRSRQADSHLGH
                     VFNDGPVDAGGLRYCINSAALDFIPYDQMAKRGYGDYLSLFDE"
     gene            4538..7330
                     /locus_tag="VBC94_001618"
     CDS             4538..7330
                     /locus_tag="VBC94_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEO6979876.1"
                     /translation="MASTNNVSDTNTRFAKEELEEVFEELGTTQGGLSDEEVAVRQKK
                     YGLNLLSEVKQESIILLFLKNFTSLMAILLWVGGFVAIVSNSLELGLAIWMVNVINGI
                     FSFIQEYRASQATQALKKMLPSYSRVLRKSSEEKVLSEQLVPGDIVLIEEGDCISADG
                     RLIKTTDLQVNQSALTGESNPIYKDSNVENYQSKTLIECDNMVFAGTTVSSGSATMVV
                     TAIGMQTQFGQIADLTQGMKSEKSPLQRELDRLTKQISIISITVGIIFFLAATFFVKE
                     PVSKSFIFALGMIVAFIPEGLLPTVTLSLAMAVQRMAKEHALVKKLSSVETLGATSVI
                     CSDKTGTLTQNEMTVNHLWQNGKSYQVTGLGYAPEGQILFEGDNICFGNSDRGDLEKL
                     IRFAHLCSNAQVLPPNDDRSTYTVLGDPTEACLNVLLEKSGINIQENRKFAPRLKELP
                     FDSVRKRMTTIHSLGGDEKDKKISITKGAPKEILDLSDYVLSDGKVIPLNKEERNKIQ
                     LANDTFAKDGLRVLAVSYCDIEGFSKEQWTQENLEQHMVFIGLIAMSDPPREGVREAI
                     DKCHAASIRIIMVTGDYGLTALSIAKNIGIIRNDDAKVISGLELSEMTDSQLKKELSG
                     EVVFARVAPEQKYRVVTILQEMGEVVAVTGDGVNDAPALKKSDIGVAMGVTGTDVAKE
                     SADMILTDDHFASIVHAVEEGRAVYQNIKKFLTYIFNSNTPEAVPSAFFLFSKGFIPL
                     PLTVMQILAVDLGTDMLPALGLGVEPPETDVMNRPPRRLTDRLLDKGLLIKSFLWYGT
                     IESVLAMGGFFWAHYLRYGNFTFFVANGIPYREATTMTLGAIIFSQIGMVMNSRTSYQ
                     SIKTLSIFGNKLINFGIIMEILAFLVLVYVPLFHNLFNTASLGLSHWLYLISCPFIVI
                     GLDEVRKLFSSRKNKR"
     gene            7330..8433
                     /locus_tag="VBC94_001619"
     CDS             7330..8433
                     /locus_tag="VBC94_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6979877.1"
                     /translation="MSNIITYLKNNSNLTFDELALNDVDILCLNEFGYISFEKLINTT
                     EMKSVLVCELYHEYLQTMAKSYSFMFTSQRHDLCQLMMTSKRFKNLTLSYYQAEISLE
                     FEKQFAAMVFTIPNIDYHQVVFRGTDANLIGWKEDFKLTYMREISAHRSAIKYLNTIL
                     PYFDKVVLSGHSKGGNLALYAAMFTKPDLKAKIDLIWLIDSPGLQKTLLPTTEYKTTK
                     QKCIRLLPEESIVGMMLYSDIEPLIISSNARGILQHDVTTWEIQEPAILKTGAGLSLK
                     SICFEKTFQQWMAELKSQERKLFFDLLFDSFLSSGVSSLDDFNLASRAKMMKAFHSFR
                     ELDDDKKRLFNKSLKLLVTIFWGAYHDNSRETK"
BASE COUNT         2926 a         1323 c         1604 g         2587 t
ORIGIN      
        1 aaagatggca aatagtattg caaaaagaaa aacatctgat ataattaact agttaactaa
       61 aacgaaaaaa catttatgga ggactcatga aaggtcaaaa aattattgct ctagctggtc
      121 ttgtcctgtc atgccatttt gctcttacgg catgtcatac tcaagaacac aaaaattctt
      181 atcatattaa aacaaagcag gttgctaaga aaaaagctaa taaaaagaaa gtctctgtta
      241 aggaaagtca taaaaaacgc aagggtgttg caggggttga ctttccaact gatgatggct
      301 ttttactgac taaagattcg aagattttat ctcatacaga ttctggaatc gttgtagcac
      361 atggaaatca ttctcatttt attttttact ctgatttaaa aggttcaaaa tttagctatt
      421 taatccctaa tgctaatgca aaaactaata aaaatcaagc ggtaagaaac tttaaagcag
      481 gggcggtagc tgttaataca ttaaatgatg gctatgtttt taatcccgca gatattgttt
      541 cagaagatgc taatggctac gtggtcagac atggtgacca tttccactat attccaaaag
      601 caagtttatc tcagcaaaag caagtacaag caagtagagc tgtttcacgt ttaggaaatc
      661 aaagtaatag ccattacagg gttaatagtt ctaaaattgc agggcttcac tacccaacaa
      721 gtgatggttt cttatttaat ggacgaggaa taaaaggcac taccccaaca ggtattttag
      781 tagaacatca taatcatctg cattttatta gttttgctga tttaagaaaa ggggggtggg
      841 gatcaattgc tgaccgttac caaccacaaa agaaagctga ttctaaaaaa cagtcaccat
      901 caagtaaaaa gccaagaaat gaaaatactt tacctaagga catcaaagat aagctagctt
      961 accttgctcg tgaattacat ttagatattt cacgtatcag agtccttaaa acactaaatg
     1021 gtgaaattgg atttgaatac ccacatgatg atcatacaca tgttattatg gccaaagata
     1081 ttgatctaag taagccaatt ccaaatccac atcatgatga cgaagatcat cacaaaggtc
     1141 atcaccatga tgagtccgac cataagcatg aagaacacga gcacactaaa tcaaataaat
     1201 tatctgatga aaatcaaaag aaattaattt atttagcaga gaagcttggt ttaaatccta
     1261 atcaaattga agtattaacg tcagaggatg gaagtatcat ctttaagtat ccacatgatg
     1321 accactcaca cactatagca agtaaggata tcgaaattgg gaagcctatc ccagatggac
     1381 accacgatca ctctcatgca aaagataaag ttggtatggc aactctaaaa caaattggtt
     1441 ttgatgatga gattattcag gatattctgc atgcagatgc tccaacacca ttcccatcaa
     1501 atgaaactaa tcctgaaaaa atgcgtcagt ggcttgcaac tgttactaag attaacattg
     1561 gacaaagaac caatccattc cagcgttttg gtctatcatt gatgcctaat attgaagttc
     1621 taggaattgg gtttactccg attaatgata tgacgccagt tttgcaattc aaaaaactta
     1681 aacagctttg gatgacaaat actggtatta cagattattc tttccttgat aagatgccac
     1741 tattagaagg attggatatt tcacaaaacg gtattaagga tttatctttc cttacaaaat
     1801 ataaacaatt aagtcttatt gctgcagcta ataatggcat tacttcgcta aaaccattgg
     1861 ctgaattacc aaaccttcaa ttcttagttt tgagtcataa caatatctct gacttgacac
     1921 cgttatcaaa cttgacaaaa ctccaagaat tgcaccttga tcataataat gtgaagaatt
     1981 taagtgcact ttctggtaaa aaagatttga aagttctaga tttatcaaac aataaatccg
     2041 cagacttatc gactctaaaa acaactagtc ttgagacgct tcttttaaat gagacgaata
     2101 caagcaatct aagtttctta aagcaaaatc ctaaggtatc taatttaaca attaataatg
     2161 cgaaattatc atcattagat ggtattgaag agagcgatga aattgttaaa gtagaagctg
     2221 aagggaacca aattaagtca ctagtattga aaaataaaca aggttcgtta aaattcttga
     2281 atgtgactaa taatcagtta acatcacttg aaggtgttaa taattatact tcacttgaga
     2341 ccttaagtgt ttctagaaat aaacttaaat ctttagatat taaaacacct aacaaaacag
     2401 ttacaaatct tgattttagc cataacaatg ttccaacatc acaattgaaa ttgaatgaaa
     2461 aaaatattcc tgaagcagta gctaagaatt ttccagcagt tgtagaaggt tcaatggttg
     2521 gaaatggtag tcttgctgaa aaagcagcta tggcttctaa agaggacaaa caagtttcag
     2581 ataatactaa tcaccaaaaa aacactgaaa aatctgctca agcgaatgct gatagtaaaa
     2641 aagaaaaccc taaaacacat gatgaacatc atgaccatga agaaacagat catgcacatg
     2701 taggtcatca tcaccattaa tcttttacgg agctaatata taaaatcctt caagaatttc
     2761 ttggaggatt ttgcatttat atgctaaatc atttattttt ttagaaaatt agctataata
     2821 tgactatggt taaaagagaa gatgtgattt taaatgcaac tgaaaatatg gtttaccata
     2881 aattaaaaaa tgatccaagt ggccatgact ggtttcatat tgttagagta agaaatcttg
     2941 cagtagaatt agcacacaaa gaaggtgcta atacctttat ttgtcaaatg gctgcacttt
     3001 tgcatgatat tattgatgac aaaatctgcc aagatagtaa gcaagcgtcg tatgaattga
     3061 ctcaatggtt atatagtcaa gatcttgcca tagcagaagt agaacatatc cttgatattc
     3121 ttgaaaatat ttcattcaaa gcaggaactg gattgactat gaaaacattg gaaggtcaaa
     3181 ttgttcaaga tgcagatcgc ctagatgcta tgggggctat tggtattgca agaacgatgg
     3241 cttattcagg gagtaaagga cgtttaattc atgaccctaa cttaaaacca cgagaaaacc
     3301 tcactttaga agagtatcgt aatggtcagg atacagctat catgcatttt tacgaaaaat
     3361 tattaaagtt aaaagacctt atgaatacaa aacaagggaa aatgcttgct caaaaacgtc
     3421 atgatttttt agaactatat ttagccgaat tttatgctga atggaatggt aaaggttagg
     3481 agtagatatg attcgtagag cgaaagaaaa agatttgcct gctattgcag agctattgaa
     3541 acaaatttta gtgttacatc atggggttcg cccagatatt tttcatacaa gaggaagtaa
     3601 gttttctaaa gaacaattga aagaaatgct gatggacgaa tcaaggccta tctttgttta
     3661 tgaatcagac gagggtaaag ttgttgccca cttgtttctt caattacaag aaaaacgtga
     3721 cttacctcga aagtccttta aaactctcta tatagatgat ttgtgcattg atgaggaagt
     3781 aagaggtcag caaattggac aaaagttgat ggattttgca agacaatatg ctaagaaaca
     3841 tgggtgttat aatataactt taaatgtttg gaatgataat cagagagcgg tctcttttta
     3901 tgaaaaatta ggttttaaac ctcaacaaac tcaaatggag gagatactat aatgaaagaa
     3961 acacaagaag aattacgtca gcgtatcgga catacagctt atcaagtaac ccaaaattca
     4021 gctacggaac atgcttttac tgggaagtat gatgattttt ttgaagaggg gatatatgta
     4081 gatatcgtca gtggagaggt tttattctct tctttagata aatttcaatc tggttgtggt
     4141 tggccagcat tttcaaaacc aattgagaat cgaatggtga caaatcatca agatcactct
     4201 catggaatgc atcgtattga ggttaggagt cgtcaagctg actctcattt agggcatgta
     4261 tttaatgatg gaccggttga tgcaggtggc ttacgctatt gtatcaattc agctgcctta
     4321 gattttattc cttatgacca gatggcaaag agaggctatg gggactactt atcacttttt
     4381 gatgaataag agacgactta agggtatatc ttaagtcgtt ttttatgtat ggtaaaaaga
     4441 attggtggca ggtgtattct tcatcagttt attttctgaa aaaagagtat aatataggta
     4501 tcaaaaatga aaacgtttta gagaaggaga ctagaatatg gcttctacaa ataatgtttc
     4561 agatacaaac acacgttttg ctaaagagga attagaagag gttttcgaag aattagggac
     4621 aactcaaggt ggtttatcag atgaagaggt tgctgtacgt cagaaaaaat atggtttaaa
     4681 cctgttatct gaagtaaagc aagaatcaat tattttattg tttctgaaaa attttactag
     4741 tcttatggca atactattat gggttggagg atttgtggct attgtttcta atagcttaga
     4801 gctaggtctt gccatttgga tggttaatgt gattaatggt atttttagtt tcattcagga
     4861 ataccgtgca agccaagcaa cacaagctct caagaaaatg ttaccatctt attcacgtgt
     4921 tcttagaaaa agctcagaag aaaaggtatt atcagagcaa ctagtcccag gagatattgt
     4981 tcttattgaa gaaggagatt gtatttcagc agatggtcgc ttgattaaaa caacagattt
     5041 gcaagtaaac caatcagctt tgacagggga atcaaatcct atttataaag atagcaatgt
     5101 tgaaaactac cagtctaaaa cattgattga atgtgataat atggttttcg caggaacgac
     5161 agtctccagt ggtagcgcaa caatggttgt tacggcaatt ggaatgcaaa cccaatttgg
     5221 tcaaatcgct gatttaacac aagggatgaa atcagaaaaa agccctcttc aaagagaact
     5281 tgatcggttg accaaacaga tttctatcat ttcaattact gtaggaatta tctttttctt
     5341 agcagcgact ttttttgtta aggagccagt gtcaaaatca tttatctttg ccttggggat
     5401 gatcgttgca tttatcccag aaggtttact acctaccgtt acactttctc tagctatggc
     5461 agtccaacga atggctaaag aacacgcact tgtcaaaaaa ctttcttcag ttgagactct
     5521 tggagcaacc tcagttatct gttctgataa gactggtaca ttaacgcaaa atgaaatgac
     5581 cgtcaatcat ttgtggcaaa atggaaagag ttaccaagtg acaggactgg gttatgctcc
     5641 agaaggacag attctttttg aaggtgacaa tatttgcttt ggtaatagtg atagaggaga
     5701 cttagaaaaa cttattcgtt ttgctcatct ctgtagcaac gctcaagtat taccaccaaa
     5761 tgatgatcgt tcaacttata ctgtacttgg agatccgaca gaagcttgtt tgaatgtttt
     5821 attggaaaaa tcagggataa acatacaaga aaatcgtaag tttgcaccac gtttgaaaga
     5881 gttacctttt gattcggtta gaaaacgcat gacaactatt catagcttag gaggcgatga
     5941 aaaggataaa aaaatttcaa tcacaaaagg tgcgccgaaa gaaatacttg atttatcaga
     6001 ttatgtctta tctgatggca aagttatccc cttaaataag gaagaacgta ataaaataca
     6061 gttggctaat gatacatttg ctaaagatgg acttcgcgtc ttagctgttt catactgcga
     6121 tattgaaggc ttttcaaaag aacaatggac gcaagaaaat ttggaacaac acatggtgtt
     6181 tatagggttg atagcaatga gtgatccacc tcgtgaaggc gttcgtgaag ctattgataa
     6241 atgtcatgca gcaagcattc gtattattat ggtgactgga gattacggat taacggcact
     6301 gagtatcgct aaaaatattg gtattattcg taacgatgat gctaaagtaa tttcaggttt
     6361 agagttatca gaaatgacgg atagccaatt gaaaaaagaa ttgtcaggtg aagtcgtttt
     6421 tgctcgtgta gcaccagaac aaaaatatcg agtagtaacg atattacagg agatgggtga
     6481 agtagtagca gttacaggag acggggtcaa tgatgcgcct gcgctaaaaa aatccgatat
     6541 tggtgtagcc atgggtgtta ccggtactga tgttgctaaa gaatcagctg atatgattct
     6601 gacagatgat cattttgctt ctattgtaca tgccgtggaa gaagggcggg ctgtttatca
     6661 aaacattaag aaattcttga cgtatatttt taatagtaat actcctgagg cggttccgtc
     6721 ggcattcttc cttttttcta aaggttttat tcctttacca ttgactgtaa tgcaaatact
     6781 tgctgttgat ttgggaaccg atatgcttcc cgctttaggt ctaggcgtgg aaccaccaga
     6841 aacagatgtt atgaatcgtc ctccaagacg gctaacagat cgcttattgg acaaagggct
     6901 acttataaaa tctttccttt ggtacggcac tattgaatcc gtattagcta tgggtggttt
     6961 tttctgggct cattacttac gttatggtaa ttttactttt ttcgtcgcaa acggcatacc
     7021 gtacagagaa gcgacaacta tgacacttgg agctattatc tttagccaga taggtatggt
     7081 tatgaatagc agaacgagct atcaatctat taaaacacta tctatttttg gtaataaatt
     7141 aattaatttt ggtataataa tggagatttt agcattttta gtcttagtat acgtgccact
     7201 attccataac ttattcaaca cagcaagcct aggtttatca cattggctct atttaataag
     7261 ctgcccgttt atcgtgatag gtttagatga agttcggaaa ttattcagca gtagaaagaa
     7321 taaacggtga tgtcaaatat aataacttat ttaaaaaata attcaaactt aacttttgat
     7381 gaattagcat taaacgatgt tgacattctt tgtttaaatg aatttggcta tatttctttt
     7441 gaaaaactta taaacactac agaaatgaag tcagttttag tctgtgagtt atatcatgag
     7501 taccttcaaa ctatggctaa atcatatagc tttatgttca cgtcacaacg acatgattta
     7561 tgtcagttaa tgatgacctc gaaacgtttt aaaaacttaa ctttatctta ttatcaagct
     7621 gagattagtt tagaatttga aaaacaattt gcagctatgg tttttactat tcctaatatt
     7681 gattatcatc aggttgtatt tcgtggtact gatgccaacc ttattggttg gaaagaagat
     7741 tttaaattga cgtatatgag agaaatatcg gctcatcgtt cagcgattaa atacttaaat
     7801 acaatactgc cttattttga taaagtagtt ctctcaggtc attctaaggg tggtaattta
     7861 gccttgtacg ctgcaatgtt tacaaagcca gatttaaaag caaaaattga tttgatttgg
     7921 cttattgact ccccaggact acaaaaaact ttgttaccta ctacagagta taaaactact
     7981 aaacaaaagt gcattcgctt actaccagaa gaatctattg ttggtatgat gctttatagt
     8041 gatatagagc cgctaattat tagtagcaat gcgcgtggaa ttctgcaaca cgatgttaca
     8101 acttgggaaa ttcaagaacc agctatttta aagactggag caggactatc gctaaaaagt
     8161 atttgttttg aaaagacatt tcaacagtgg atggctgaat taaaaagtca agaaagaaaa
     8221 ttattttttg atttattatt tgatagcttc ctatcaagtg gcgtatctag tttggatgac
     8281 ttcaacttgg cttccagagc aaaaatgatg aaagcttttc atagctttag agaactagat
     8341 gatgataaaa aaaggttatt taacaaatct ttaaaattat tagtaaccat attttgggga
     8401 gcttatcatg ataatagcag agagactaag tagctttcct
//
LOCUS       DATROQ010000079         8424 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.080, whole genome shotgun sequence.
ACCESSION   DATROQ010000079 DATROQ010000000
VERSION     DATROQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.080"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            72..989
                     /locus_tag="VBC94_001620"
     CDS             72..989
                     /locus_tag="VBC94_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEO6979878.1"
                     /translation="MKDLQKYQGIIPAFYACYDDKGDICPERVKALTNYFIDKGVQGL
                     YVNGSSGECIYQSVADRKLVLENVMSVAKGKLTVIAHVACNNTKDSVELAMHAEAMGV
                     DAIAAIPPIYFRLPEYAIADYWNTISQAAPQTDFIIYNIPQLAGVALTSDLYRKMLQN
                     PQVIGVKNSSMPVQDIQNFVAIGGENHIVFNGPDEQFLGGRLMGAAAGIGGTYGVMPE
                     LYLTLNQLIVDKDLEKARELQFTINDIITKLCSGHGNMYAVIKAVLEINEQLTIGSVR
                     LPLASVTEEDKPIIKEAAEMIRHAKKQFC"
     gene            1006..1887
                     /locus_tag="VBC94_001621"
     CDS             1006..1887
                     /locus_tag="VBC94_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO6979879.1"
                     /translation="MTRTVAIDIGGTMIKHGIVDNLGCIVEASELATEAYKGGPGILQ
                     KVCQIIDNYLTEGSIDGIAISSAGMVDPDEGCIFYSGPQIPNYAGTQFKKVLEDTYQV
                     RTEIENDVNCAGLAEAVSGSAKDSSIALCLTIGTGIGGCLIIDKTVFHGFSNSACEVG
                     YMHLSDGDFQDLASTTALIADVAKAHGDEINRWDGRRIFQEAKEGNEKCIASIDRMIN
                     YLGQGIANMVYVVNPEKVVLGGGIMAQKDYLQDKLSESLKRNLVTSLAEKTIIVFAQH
                     ENQAGMLGAYYHFKNRG"
     gene            1895..2872
                     /locus_tag="VBC94_001622"
     CDS             1895..2872
                     /locus_tag="VBC94_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO6979880.1"
                     /translation="MIETMSLDDMREYLGQDQIPEDFDDFWKKQTMKYQGNIEYRLDK
                     KDFNITFAQAYDLHFKGSNNSIVYAKCLFPKTNKPYPVVFYFHGYQNQSPDWSDQLNY
                     VAAGYGVVSMDVRGQAGQSQDKGHFDGITVKGQIVRGMISGPNHLFYKDIYLDVFQLI
                     DIIATLESVDSNQLYSYGWSQGGALALIAAALNPKIVKTVAVYPFLSDFRRVLDLGNV
                     SEPYDELFRYFKYSDPFHKTENNVLKTLAYIDVKNFAHRISCPVVLLTALKDDICPPS
                     SQFAIFNRLTSTKKHLLLPDYGHDPMTVQVKDYIFDQLTGSQFTKQKIE"
     gene            complement(2895..3698)
                     /locus_tag="VBC94_001623"
     CDS             complement(2895..3698)
                     /locus_tag="VBC94_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEO6979881.1"
                     /translation="MTIYDQIESALDLMTDLEHEIACYFMGQPISKDALASSIVTKQL
                     HISQAALTRFAKKCGFKGYREFVFEYLKSHETISQQLYGLQNDNTKKVFMNYQEMVSK
                     SADIIDEEQLLEVSHMIEQADRVYFYGKGSSSLVAKEFKIRLMRLGVICEALDDTDSF
                     SWTNSIVNDRCLVIAFSLSGNTNSVIGALKIASCHGAKTVLFTKQPHTIDYAFDKIIQ
                     VASARHLDYGNRISPQIPMLIMVDIIYAQFLDINKIEKERIFRETIIQR"
     gene            3980..5242
                     /gene="purD"
                     /locus_tag="VBC94_001624"
     CDS             3980..5242
                     /gene="purD"
                     /locus_tag="VBC94_001624"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEO6979882.1"
                     /translation="MKLLVVGSGGREHAIAKKLLVSKGVDQVFVAPGNDGMTLDGLGL
                     VNIVISEHSRLIDFAKENEIAWTFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTYSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPTAQDHKCAY
                     DGDMGPNTGGMGAYAPVPHLPQSVVDTAVETIVKPILEGMITEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFAENGKALLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIK"
     gene            5528..6016
                     /gene="purE"
                     /locus_tag="VBC94_001625"
     CDS             5528..6016
                     /gene="purE"
                     /locus_tag="VBC94_001625"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEO6979883.1"
                     /translation="MQPIISIIMGSKSDWTTMQKTAEVLDNFGIAYEKKVVSAHRTPD
                     LMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSNE
                     LI"
     gene            6003..7076
                     /gene="purK"
                     /locus_tag="VBC94_001626"
     CDS             6003..7076
                     /gene="purK"
                     /locus_tag="VBC94_001626"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEO6979884.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPASDCPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSAGQLPQGTDLLRISQNR
                     IFEKDFLANKAGVTVAPYKVVTSSLDLEGLDLTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAECVLEEFVDFDLEISVIVSGNGKDVTVFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKELQLSGTLCVEMFVTADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGQPLPPIKLHDPAVMLNVLGQHVQQAIDYVAQNPSAHLHMYGKLE
                     AKHNRKMGHVTVFVEDADEVEEF"
     gene            7086..8330
                     /locus_tag="VBC94_001627"
     CDS             7086..8330
                     /locus_tag="VBC94_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEO6979885.1"
                     /translation="MGLFGFGKPKDEALEAKISSLESKLKDKQSILDDLTRQIKVAKD
                     ILKLDDELKKRQSELKALQKEVIVLNDDLDLQEYGFFERKYKFSDSTKYKEELETLRK
                     RQKQLVKDKKAGVIVRPMLLDNSKSKGRAMQNQLIKAMIRGFNGEVDAHLVKVTATNL
                     SKKVDAVVKSFNQLNKMYSRNLVCLSNDYLELKISELQLAVEYELQKLEEKELLREQR
                     AKEREDKKLQAELAAKRKQIEKDRKHFKQMLDNVEELLKTANEEEKEKLNLQLAEYQD
                     KLSELDELEEDIDYREGHATAGYVYIISNIGAFGEDVYKIGVTRRLDPMERIRELGSA
                     SVPFQFDVHALIFSEEAFSLEKELHNRFEKNKINKVNGRKEYFKVPFSDIKAVLEEHK
                     ELAIELTEKAEAFEYRQSLMTK"
BASE COUNT         2653 a         1494 c         1840 g         2437 t
ORIGIN      
        1 tttgcttact ttaaaattat tattttacaa aaacaatttg cctatttcag caaacaacaa
       61 ggggattatc aatgaaagat ttacaaaaat atcaaggaat tattcctgca ttttatgctt
      121 gctatgatga taagggtgat atttgtcccg aacgagtaaa agccctgacc aattatttta
      181 ttgataaagg tgttcaagga ctttatgtta atggttcatc aggagaatgt atttaccaaa
      241 gtgtagcaga tcgtaaactt gtactggaaa atgttatgtc cgttgctaag ggtaaactaa
      301 cagtgatagc gcatgtagca tgtaataata ctaaggatag cgtcgagttg gctatgcatg
      361 ctgaagctat gggtgttgat gctattgcag caattccacc aatttatttt cgattaccag
      421 agtatgccat agcagattat tggaatacta ttagtcaagc agcaccacaa actgatttta
      481 ttatttacaa tattcctcaa ttggcagggg ttgctttgac atcggattta tatcgaaaaa
      541 tgttacaaaa tccgcaagta attggtgtta aaaattcttc catgccagtt caagacattc
      601 agaattttgt ggctattgga ggagaaaatc atattgtttt taatggtcca gatgaacaat
      661 ttttgggtgg tcgtctcatg ggagcagctg caggtattgg tggtacctat ggcgtaatgc
      721 cagaattata cttgacttta aatcagttaa ttgttgataa agatttagaa aaagcgcgtg
      781 aacttcaatt tacaattaac gatattatca caaagctttg ttcgggtcat ggtaatatgt
      841 atgctgtgat caaagctgta ttggaaatca atgagcaact aactataggt tccgttcggt
      901 tacctttagc atctgtaaca gaagaagata aacctattat taaagaggca gcagaaatga
      961 ttagacatgc taaaaaacag ttttgttaaa gtgaggtaga taattatgac cagaacagta
     1021 gcaattgata ttggcggtac aatgattaag catggcattg tggataattt aggttgtatt
     1081 gttgaggcaa gtgagcttgc cacagaagcc tacaaaggtg gtccaggcat tttacaaaaa
     1141 gtttgtcaaa ttatcgataa ctatctaaca gaagggagca ttgatggaat tgctatctca
     1201 tctgctggca tggtagatcc agatgaagga tgcatatttt actctggtcc tcaaattcca
     1261 aactatgctg ggacacaatt taagaaagtt ttagaagaca cttatcaagt acgcacagaa
     1321 attgaaaatg atgttaattg tgcaggatta gcggaagctg tctcaggtag tgccaaagat
     1381 agttcaatag cgctttgtct tactattgga acaggtattg gtggttgtct gattattgat
     1441 aaaacagttt ttcatggctt tagtaattct gcttgtgaag ttggctatat gcacctttca
     1501 gatggtgatt ttcaagattt agcctctaca actgcactga ttgctgatgt tgccaaagcg
     1561 catggagatg aaattaaccg atgggatgga cgtcgtattt ttcaggaggc taaagaaggc
     1621 aatgagaaat gtattgcaag tatagaccgt atgataaact accttggtca aggaatcgct
     1681 aatatggttt atgtggtaaa tcctgaaaaa gttgttttag gtggtggaat catggctcaa
     1741 aaagattatt tacaagataa acttagtgaa tctttgaaac gaaatcttgt gacaagtctt
     1801 gctgaaaaaa caataatagt ttttgcacaa cacgaaaacc aagctggtat gctaggagct
     1861 tactaccatt ttaaaaacag ggggtgacgg gtatatgatt gaaacaatgt cgctagatga
     1921 catgagagaa tacctaggtc aagaccaaat tcccgaggat tttgatgact tctggaaaaa
     1981 acaaacaatg aaatatcaag ggaatattga gtatcgtcta gacaaaaaag atttcaatat
     2041 tacttttgca caagcttatg atcttcattt taaaggcagc aataactcta ttgtctatgc
     2101 caaatgttta ttccctaaaa caaataaacc ctatcctgta gttttttatt ttcatggtta
     2161 ccaaaaccaa agtcctgact ggagtgacca acttaattat gtagcagcag gttacggcgt
     2221 tgtttcaatg gatgttcgag gtcaagctgg acagtctcaa gacaaggggc attttgatgg
     2281 cattacagtt aagggccaga ttgtgcgagg aatgatctca ggaccaaatc atctttttta
     2341 taaagacatt tatcttgatg ttttccaatt gattgatatc attgctacat tagagagtgt
     2401 tgatagtaat caactttata gttatggctg gtctcaaggt ggagcacttg ctttgatagc
     2461 tgcagctctt aatcccaaaa tcgtcaagac cgttgctgtt tacccatttt tgtcagactt
     2521 tagacgagta ttagatttag ggaatgtcag tgagccttat gacgaactct tccgctattt
     2581 taaatatagt gatccttttc ataaaacaga aaataatgtg ttaaaaactt tggcttatat
     2641 tgatgttaaa aattttgcac ataggatttc ttgccccgtt gttttactaa cagccttgaa
     2701 agatgatatt tgtcctccat caagtcaatt tgccattttt aaccgtttga catcaactaa
     2761 aaagcatctt ttattaccag actatggaca tgatccaatg acagtacaag taaaagacta
     2821 tatttttgat caactgacag gaagtcaatt tacaaagcaa aaaatcgagt agattatttc
     2881 tactcgattt tcatttagcg ttgaataatg gtttctctaa aaattcgctc tttttctatt
     2941 ttattaatat ctaaaaattg tgcatagata atatctacca tgattaacat tgggatctga
     3001 ggcgaaattc tattgccgta gtctaaatgc ctagcagagg ccacctgaat aatcttgtca
     3061 aaagcatagt caattgtgtg aggttgtttt gtaaatagaa cggttttagc tccgtgacaa
     3121 cttgcgattt ttaaggcacc gataacagag ttggtgttcc cagaaagtga gaaggcaatg
     3181 acgaggcatc tatcattaac aatactatta gtccatgaga aactatctgt atcatccaga
     3241 gcctcacaaa tcactcccag cctcataagg cgtattttaa attctttagc tactagcgat
     3301 gagctccctt tgccataaaa ataaacacga tcagcttgct caatcatatg tgatacttct
     3361 agaagctgtt cttcatcaat aatatcagcg gatttagaaa ccatctcttg ataattcata
     3421 aatactttct tagtgttgtc attttgaaga ccgtataatt gttgactgat ggtctcatgg
     3481 ctttttaaat attcaaaaac aaattcacga tagcctttaa acccacattt tttagcaaaa
     3541 cgcgttaagg ctgcttgtga aatgtgtagt tgcttagtga caatggaaga agcaagagcg
     3601 tcttttgaaa tgggctgccc cataaaataa caagcaattt catgttctaa atcagtcatt
     3661 aaatcaagag cagactcaat ttggtcataa atagtcataa tgcacggtcc tctcctcttt
     3721 aagaaagcgc ttaacatatt cagtataaca taaagttagc gaggggccaa actaagttct
     3781 aagaaacagc aatgataagt tttaaatagc aatatgaagg attaataaca taatatatgt
     3841 gaagtatata ttatgttatt aatttttcaa aaaagatgca acaaaaaact taaaagcgaa
     3901 caaatgtgtt ataattttat aaataatacg tttttattgc aaatttcttt ttatattatt
     3961 taataggagt tattctgata tgaaattgct tgttgttggt tctggtggtc gtgagcatgc
     4021 gattgctaag aagttgttag tgtctaaggg tgtagatcag gtttttgtgg cacctggtaa
     4081 tgatggtatg accttggatg gtctaggctt ggtaaatatc gtaatttccg aacattctag
     4141 gctgattgac tttgctaagg agaatgagat tgcttggact tttattggtc ctgatgatgc
     4201 gctagcggct ggtatcgttg atgatttcaa cagtgctgga cttagagctt ttgggccaac
     4261 aaaggcagca gcggagctag agtggtcaaa agactttgct aaggaaatca tggttaaata
     4321 caacgttcca acagcagcct atggcacata ttcagatttt gaaaaagcta aagcctacat
     4381 cgaagagcag ggtgcaccaa tcgtggtcaa ggctgatggc ttggcgttag gcaagggcgt
     4441 ggtcgtggct gaaaccgttg agcaggcggt agaggcggcg caagagatgc ttttggacaa
     4501 caagtttggc gactcaggag cgcgtgtggt tatcgaggaa tttttggatg gtgaagagtt
     4561 ctccctcttt gccttcgcta atggcgacaa gttttacatc atgccgacag ctcaggatca
     4621 caagtgtgcc tatgacggtg atatgggacc aaacactggc ggtatgggtg cctatgcgcc
     4681 agttccccac ctgcctcaga gcgtagtgga tacggcggtt gaaaccatcg ttaagcctat
     4741 ccttgagggt atgattactg aagggcgtcc ttatctaggc gtcctctatg cggggcttat
     4801 cctgacggct gatggtccga aggttatcga gttcaactca cgtttcggtg acccagaaac
     4861 tcagattatc ctccctcgcc tgacttctga ttttgctcag aacatcgacg acatcatgat
     4921 gggcatcgag ccttacatca cttggcagaa ggacggcgtg actttgggcg ttgtcgttgc
     4981 ctcagaaggc tatccgctcg attacgagaa aggtgtgcca ctgcctgaaa agaccgacgg
     5041 cgacatcatc acctactatg ccggagctaa gtttgcggaa aatggcaaag cactgctctc
     5101 aaacggtgga cgagtctata tgcttgtcac cacagaagac agcgtcaaag cagggcagga
     5161 caaaatctat acccaactag cccaacaaga caccacaggc ctcttctacc gaaacgacat
     5221 cggaagcaaa gctattaagt aataagacta ctcattcaaa tattagcgtc agcaatcagc
     5281 gaccgaaggt cgctccaaaa agaaagaaga aaggaactga acacgggact aattttctag
     5341 tgaaaaagat aaatcttcct agatgctaaa gcatcttcgt cagatttttg cgtcgtaggc
     5401 gtctgctgct aaagcaccta gtcgccctga cgtgggtgcg tcttcgaaat caaagatttc
     5461 ggacttaccg catatttttc tacgaaaatt ttcgacaagt cgaaacgtcc ctttgtatct
     5521 taatattatg caaccaatta tttctattat catgggttcc aaatccgact ggacaaccat
     5581 gcaaaaaacg gctgaagttc tagataactt tggcattgct tacgagaaga aagtcgtctc
     5641 tgcccaccgt acgccagacc tcatgttcaa gcatgctgag gaggcgcgtg gtcgtggtat
     5701 caaaatcatc attgctggag caggtggggc agcccacttg cctggtatgg tagcagctaa
     5761 aacaaccctg cctgttatcg gtgtgcctgt caaatcacgt gccctttcag gcttggattc
     5821 gctctattcc attgtgcaga tgcctggcgg tgtgcctgtg gcaaccatgg ctatcggaga
     5881 agcgggagcg actaacgctg ccctaacagc cctccgtatt ctctctatcg aggaccaaaa
     5941 tctggctgat gcgctagctc atttccatga ggaacaagga aaaatcgcag aggagtcaag
     6001 caatgaactc atttaagacc attgggatta tcggtggtgg tcagctgggg cagatgatgg
     6061 cgattgcggc tatctacatg ggccacaagg tcattacgct ggatcctgct agcgactgcc
     6121 ctgcctcccg cgttagcgag gtgattgtgg cgccttacga tgacgttgag gctttgggac
     6181 aattagctgc gcgttgtgat gttttgacct atgagtttga aaatgttgat gccgatggtc
     6241 tggatgccgt tgtgtcagct ggtcagctac cgcaggggac tgatctgctc cgcatttctc
     6301 aaaaccgtat ctttgaaaaa gactttctgg caaataaggc tggagtgact gtcgctccct
     6361 ataaggtggt gacgtctagc cttgacctag aggggcttga cttgaccaag acctatgtcc
     6421 tcaagacagc gacaggtggt tatgacggtc atgggcaaaa ggttatccgc tcagcagaag
     6481 acctgccaga ggcgcaacaa ttagccaact cagctgagtg tgtcttggaa gagtttgtcg
     6541 actttgacct tgaaatctca gtcatcgtgt ctggaaatgg caaggatgtg acggtctttc
     6601 ccgttcagga aaatatccac cgcaacaata tcctatcaaa aactatcgta ccagctcgca
     6661 tctcagacca actagctgac aaggctaaga aaatggcggt acagattgcc aaggaactcc
     6721 agctatcagg aaccctctgt gtggaaatgt ttgtgaccgc agatgatatc atcgtcaatg
     6781 aaatcgcccc acgcccccac aactcagggc actattctat cgaagcctgt gacttttcac
     6841 agtttgacac ccacatcttg ggtgtactgg ggcaacctct cccaccaatc aagctccatg
     6901 acccagccgt tatgcttaat gtccttggac aacatgtcca gcaggcgatt gactatgttg
     6961 cccaaaaccc tagcgctcac ctccacatgt atggtaaact agaagcgaaa cataaccgca
     7021 aaatgggaca tgtaacggtg tttgtggaag atgcggatga ggtggaggag ttttaggggg
     7081 aataaatggg tctatttgga ttcggaaagc ctaaagatga ggctttggaa gctaaaatca
     7141 gtagtttaga aagtaagtta aaagataaac aatctatttt ggatgattta acaaggcaga
     7201 taaaggttgc taaagatatt ttaaaacttg atgatgaact taagaaacgc caatcagaac
     7261 ttaaagcttt acaaaaagag gtaatagttc ttaatgatga tttagatctt caagagtacg
     7321 gattttttga gagaaaatat aagttctcag atagtactaa gtataaagaa gaattggaaa
     7381 ctctgagaaa gaggcaaaaa cagttagtaa aagataaaaa agcgggtgtt attgtaagac
     7441 ctatgttgct ggataacagt aaatcaaaag gacgagcaat gcaaaatcaa cttattaaag
     7501 cgatgataag aggttttaat ggagaagttg atgctcacct ggtaaaggta acggctacta
     7561 atctaagtaa aaaagtagat gcagttgtta aatcatttaa tcagcttaat aagatgtatt
     7621 caagaaattt ggtatgtcta tctaatgact atcttgagct aaaaatttca gagttacaat
     7681 tagcagtaga atatgaactt cagaaattag aagaaaaaga acttcttcgt gaacaacgtg
     7741 cgaaggaaag agaagataag aaacttcaag cagaattagc tgctaagaga aaacaaattg
     7801 aaaaagatag aaaacacttt aaacagatgt tagataacgt tgaagaatta cttaaaacag
     7861 ctaatgagga agaaaaggaa aaattaaatc ttcaattagc agaataccaa gataaattat
     7921 cagaactaga tgagttagaa gaagatattg attatcgaga agggcatgca actgctggat
     7981 atgtttatat catttcaaat attggtgcat ttggagaaga tgtttacaag attggtgtta
     8041 caaggcgtct agatcctatg gaacgtattc gcgaattagg gagtgcatct gttccatttc
     8101 aatttgatgt acatgcctta atttttagtg aggaagcttt ttcattagaa aaagagttac
     8161 ataacagatt tgaaaaaaat aaaattaata aagttaatgg gcgaaaagag tattttaaag
     8221 tcccattttc tgacatcaaa gcagtgttag aggagcataa agaacttgct atcgagttga
     8281 ctgaaaaagc agaagcattt gaatatagac aaagtctgat gacaaaataa ttatatagaa
     8341 aagactgtat ttgtatataa cagaatatag ttgtagcttt gttgaaaaga taattgtgac
     8401 ttatttgtag gcatctttca ttcc
//
LOCUS       DATROQ010000080         8200 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.081, whole genome shotgun sequence.
ACCESSION   DATROQ010000080 DATROQ010000000
VERSION     DATROQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.081"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            125..1408
                     /locus_tag="VBC94_001628"
     CDS             125..1408
                     /locus_tag="VBC94_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO6979886.1"
                     /translation="MSLYYTEKAHAIAGPSDRQYVENPNPHIIVNVTGTDQNGNSILP
                     HYIEVNVKMGQTLSKEEILDYIARNLNSSVGGESKNVQYSNIEFKESAYLKRQLDDGK
                     TEEIAIDNDGVTVPKDGPNKFWIDVPVTCTVTPIVTETHEVRWGTPVAISHRIYFVEE
                     SSGKVLDEYTNLHTADSELNGYRVGDYITDYALSKSAYEAFLNSRLDKEGYKLQHRIS
                     TNVRQNLQINKLIFNYDFNEENIYYQIGNIRPLLSRSSAEVESDIITERYYVSKNAKS
                     LARTESTISIKMVDAKTEQPLFNHTLTGYQLVTVSHVYNRLFEENLIPTTKSGERYFI
                     QNMKKTAEQEYTVYLSETPYSKENAPVISYDARPVDWDYHSGASGSLENQPNIYTEED
                     STEFLGNKPQAACYPNKQFACENTDSKYNYSYLEK"
     gene            1563..1802
                     /locus_tag="VBC94_001629"
     CDS             1563..1802
                     /locus_tag="VBC94_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6979887.1"
                     /translation="MTLFLNSLLKELIIVNRLREYRIKASISQLALAKSVGVARQTIN
                     LIENNKYNPSLDLCIRLAECLQTDLNTLFWDSRHL"
     gene            1902..2435
                     /locus_tag="VBC94_001630"
     CDS             1902..2435
                     /locus_tag="VBC94_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEO6979888.1"
                     /translation="MKEKKNLGIKLIKSLYGINGPFDEYREQKINYLGNLLFATTSLY
                     HILSSIIAWFTVSKYPTETACTLLALTFFLYFCIFLIVIPSYLNSHDLITPLEVSKND
                     YPVAIKKNKQKFIKIGIFYTVIQWLILGLTTNNNTPLIVSLFSLKVILSSIMSGVAYN
                     GVMYLIFKNNVDIEKDE"
     gene            complement(2511..3005)
                     /gene="tpx"
                     /locus_tag="VBC94_001631"
     CDS             complement(2511..3005)
                     /gene="tpx"
                     /locus_tag="VBC94_001631"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEO6979889.1"
                     /translation="MTTFLGNPVTFTGKQLQVGDIAKDFLLIATDLSQKSLKDFEGKK
                     KVISVVPSIDTGICSKQTRTFNEELSELDNTVVITVSMDLPFAQKRWCSAKGLDNVIL
                     LSDFYDHSFGQEYALLMNEWHLLTRAVLILDEHNKVTYTEYVDNVNSDVDYEAAINAA
                     KILP"
     gene            complement(3078..3737)
                     /locus_tag="VBC94_001632"
     CDS             complement(3078..3737)
                     /locus_tag="VBC94_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEO6979890.1"
                     /translation="MDLFLSLERKFKAASDKEVSKQQETYLRHHFKCYGIKSPERRML
                     YKELIKAAKRQAKIDWQLLDKCWQSDYREYHHFVLDYLLAMSQFLTYNDCSRLEFYAR
                     HQQWWDSIDVLTKIFGNLSLKDDKVMNLLSEWSLDQDFWMRRLAIEHQLGFKEKTNTD
                     ILSLFILRNTGSQEFFINKAIGWALRDYSKYNKVWVKDFISNHYDELSTLSIREGSKY
                     L"
     gene            complement(3737..4501)
                     /locus_tag="VBC94_001633"
     CDS             complement(3737..4501)
                     /locus_tag="VBC94_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO6979891.1"
                     /translation="MDFVFDIDGTLCFDGMSLSKEIQGILERAQIDYGHRVTFATARS
                     YRDTIGILGDKLSLSKIIGLNGATLHENGHLVDSYYLQSDFFSTIISYCHRYQIPYFV
                     DEVFNYATYQASKIPFIAYVDPQKRGELLEVSKIEKPIKMVLYFGDQLRRADQMLAKL
                     NRFGLSSHFFHEFEKCLYINPIAVDKGKATKKLFGNRFIAFGNDKNDISMFDAAHYSV
                     QVGDFDELTPYANLRVSRESVHEGITMLFEKYKGIT"
     gene            complement(4506..5147)
                     /locus_tag="VBC94_001634"
     CDS             complement(4506..5147)
                     /locus_tag="VBC94_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6979892.1"
                     /translation="MVSEQSEIVTSKYQKIAVAVAQRIANGDYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLQILTLKHGSGAIILSKEKAIAFLNQYETSHSVAILKGKIR
                     DNIKAQQQEMEELATLVDDFLLQTRAVSKQYPLAPYEIIVSEDSEHLGKSIGELNVWH
                     QTGATIVAIEHEGKFIVSPGPFSVIEQGDHIFFVGDEDVYARMKTYFNLRMGL"
     gene            complement(5311..5862)
                     /locus_tag="VBC94_001635"
     CDS             complement(5311..5862)
                     /locus_tag="VBC94_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6979893.1"
                     /translation="MENQKQDIKTSVTYEEKVIAKIVGHALESVDGLLAVSGGFFSNL
                     KNSVVNSDSVTDGVNVEVGTKEVAVDLDIVVEYGKDIPAIVESIKAIVSQNVEVMTHL
                     KVVELNANVVDVKTKAEHEADSVTVQDRVSDAAQATGNFASEQAGKAKAAISSGAEKT
                     KEAVSNGTEAAKEKISEARTSES"
     gene            complement(5884..6081)
                     /locus_tag="VBC94_001636"
     CDS             complement(5884..6081)
                     /locus_tag="VBC94_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6979894.1"
                     /translation="MSEEKFDAKVDKVSGSVKESVGKLTGDKEVESEGKVDKLKGHAK
                     EKLADIKDTIKGASESFKKKD"
     gene            complement(6128..6670)
                     /locus_tag="VBC94_001637"
     CDS             complement(6128..6670)
                     /locus_tag="VBC94_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6979895.1"
                     /translation="MTETYIKNTTNNSGTTAVRGELTFEDKVIEKIVGIAIEHVDGLL
                     AVNGGFFSNLKNSVVNSDSVTDGVNVEVGKKQVAVDLDIVAEYQKHVPTIFADIKKVV
                     EAEVKRMTDLEVVEVNVNVVDIKTRAQHEEDSVTLQDRVTSAAQATGEFASNQVSNVK
                     SAVGNGVDKVEDMKSEPRVQ"
     gene            complement(6726..6920)
                     /locus_tag="VBC94_001638"
     CDS             complement(6726..6920)
                     /locus_tag="VBC94_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEO6979896.1"
                     /translation="MSEFVRKYRYPLGGAVIGLVLAAMIVTIGFFKTILALVIIVLGA
                     YAGLYVQRTGMLDQFFNKRK"
     gene            complement(6930..7511)
                     /gene="amaP"
                     /locus_tag="VBC94_001639"
     CDS             complement(6930..7511)
                     /gene="amaP"
                     /locus_tag="VBC94_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEO6979897.1"
                     /translation="MSKGLKSLYTLLGLISLTLLGFVAVISKQHIYLPSFNWLDWDFN
                     LPSPIDVGMYHYFFWGALVLFVIVLLAILVVLFYPRRYTEYKLADKTGKLMLKKSAIE
                     GFVKTEVLKTGLMKSPSVTAHLYKKKVKVDVKGLLTSRTNVPEQLEHIQSDVELGLKE
                     FFGLEKKMNTRVFVKQVEEENVGNAKTNKSRVE"
     gene            complement(7526..7774)
                     /locus_tag="VBC94_001640"
     CDS             complement(7526..7774)
                     /locus_tag="VBC94_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO6979898.1"
                     /translation="MSLIWSLIVGAIIGAIAGAVTNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLAGMALIPSIVGAIIVVIVTSFVLGKMNN"
     gene            complement(7822..8067)
                     /locus_tag="VBC94_001641"
     CDS             complement(7822..8067)
                     /locus_tag="VBC94_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO6979899.1"
                     /translation="MGLIWSLIVGAIIGAIAGAITNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLADMALIPSIVGAIIVIIVTSFVLGKKS"
BASE COUNT         2742 a         1624 c         1147 g         2687 t
ORIGIN      
        1 atcattcact gctttatttt aggataacta aacaatttat tgtttaaaat gaaaggattt
       61 atgaatgtct aggaaaacat ttaaacgtat actatcgatt ggagtttgca cgctcgtact
      121 atcgatgagt ctttattaca ctgaaaaagc ccacgctatt gctggtccta gtgaccgcca
      181 atacgtagaa aacccaaatc ctcacattat tgtaaatgtt acaggtactg atcaaaacgg
      241 aaatagcatt ttaccgcatt atatcgaagt caatgtaaag atgggacaaa ctttaagtaa
      301 agaagaaatt ctagattata ttgctcgaaa tttaaactct agtgttggag gagaaagtaa
      361 aaacgttcaa tacagcaaca tcgagtttaa ggaaagtgct tatctgaagc gtcaattaga
      421 tgatggcaag acagaagaaa tagcaattga taacgacggt gttactgtac ctaaagacgg
      481 tccaaacaaa ttttggattg acgttccagt aacttgtact gttactccta tcgtaacaga
      541 aacacatgaa gttcgatggg ggactccagt cgctatatca caccgtattt actttgttga
      601 agaatcttct ggaaaagttt tagatgaata cacaaatcta cacactgctg attcggaact
      661 taacggttat cgtgttggag attatatcac agactatgca ctttctaagt ctgcttacga
      721 agctttttta aactctcgtt tagataaaga aggttacaaa cttcaacatc gtattagcac
      781 gaatgtacga caaaaccttc aaattaataa attgattttc aattatgact ttaatgagga
      841 aaatatttac taccaaatcg ggaatatccg tccactatta agtcgctcat cagctgaagt
      901 agaatctgac atcattacag aacgctacta tgtttctaaa aatgctaaaa gtttagcacg
      961 tacagaatca accatttcga ttaaaatggt tgatgccaaa actgaacaac cgctatttaa
     1021 ccacacatta actggttatc aattggtaac tgtctcccat gtctataaca gactctttga
     1081 agaaaatctt atcccaacta caaaatcagg agaaagatat tttattcaaa atatgaaaaa
     1141 aacagctgaa caagaatata ctgtttatct ttcagaaaca ccttattcta aagagaacgc
     1201 tcccgtaatt tcttatgatg caagacctgt tgattgggat tatcactcag gcgcttcagg
     1261 atcacttgaa aatcagccta acatctatac tgaagaagat tcaactgaat ttttgggtaa
     1321 taaaccacaa gcagcttgtt atccaaacaa acaatttgct tgcgaaaata ctgactctaa
     1381 atacaactat agctatttag aaaaatagtt ataaccgata gggttaatct ctcattatct
     1441 tatactgatt aaaatcatta aacttgtttc cattttgact ggaaacaagt tttttatatt
     1501 tatttgtcac atataattga cactcttcga aaaatgatat atacttaaaa tgtcatctat
     1561 atatgacact tttcttaaat tctttactaa aggagctaat aatcgtgaat cgtcttagag
     1621 aatacagaat taaagctagc atatctcaat tagctttagc aaaatcagtt ggtgtcgcca
     1681 gacaaacaat aaaccttatt gaaaataata aatacaatcc ttcgctagat ctttgtataa
     1741 gactagcgga atgcctacaa acagacctca atactctttt ttgggatagt agacatctat
     1801 aacagattag actctctaaa tctatgaaaa ctcgggaggt taggtcgtat tattatttca
     1861 atctcaaact ttattaaata gttcaaggat aggagaatat tatgaaagaa aagaaaaatt
     1921 tggggataaa attaataaaa tccctatatg gtattaatgg cccattcgat gaatacagag
     1981 aacaaaaaat caattacctt ggaaatctat tatttgcaac cactagtctc tatcacattt
     2041 tatcctctat catcgcctgg ttcacggtaa gtaaatatcc cacagagact gcatgtactc
     2101 tcctagcttt aacattcttt ctatattttt gtatttttct aattgttatt ccctcttatc
     2161 tcaacagtca tgatttaata acgccactag aagtctcaaa aaatgactat ccagtagcaa
     2221 taaagaaaaa caaacaaaaa tttatcaaaa tcgggatatt ttatactgtc atccagtggc
     2281 ttatcttggg tttaacaact aacaataaca ctccgctgat tgtatcacta ttttctttaa
     2341 aagttatttt gtctagtatt atgtccggtg tagcttacaa tggagttatg tatctaatat
     2401 ttaaaaacaa tgttgatatt gaaaaagatg aataatgaca acatttcatc tctaattgtt
     2461 ctcactgtct atataaataa taaggctgga aaattttcca gccttattat ttatggtaat
     2521 atctttgcag cattaatcgc cgcttcgtag tcaacatctg aattaacatt gtcaacgtat
     2581 tcggtgtaag tcaccttatt atgctcatcc aaaataagaa ctgcacgcgt taacaaatgc
     2641 cactcattca tcaaaagagc gtactcttga ccaaacgaat gatcataaaa atctgataat
     2701 aaaatcacgt tatcaagacc tttagcgctg caccaacgct tctgagcaaa tggaaggtcc
     2761 atagaaacag taataaccac tgtgttatct agctctgata attcctcatt aaaagtacgt
     2821 gtttgttttg aacaaatacc tgtatcaata gaaggaacaa cgctaataac tttctttttc
     2881 ccttcaaaat cttttaaaga tttttgagat aagtctgtag ctattagtaa aaaatctttt
     2941 gcaatatccc caacttgtag ctgttttcct gtaaaagtaa caggatttcc aagaaatgtc
     3001 gtcataagtt tatctcctta tcaattacct ttattttact atgaagcttc ttctttttcg
     3061 aataattgaa ctgataatta gaggtactta cttccttcgc gtatgctcaa agtcgacaac
     3121 tcatcataat gattactgat aaagtccttt acccatactt tattatactt tgaataatcc
     3181 ctaagcgccc agccaatcgc cttattgata aaaaattctt gacttcctgt atttcttaaa
     3241 ataaaaagtg acaaaatatc agtatttgtt ttttctttaa atcctagttg atgctcaata
     3301 gctagtcgcc tcatccaaaa atcttgatca agtgaccatt ctgacagtaa attcataacc
     3361 ttgtcatctt ttaaggataa gttcccaaaa atcttagtca gaacatcaat actatcccac
     3421 cactgttgat gacgagcgta aaactctagt ctagagcaat cattataggt gagaaattga
     3481 ctcatagcta atagataatc aagtacaaaa tgatgatatt cacgatagtc actttgccaa
     3541 catttatcaa gcaattgcca atcaattttt gcttgtcttt tagcggcctt tataagctct
     3601 ttgtagagca tacgtcgctc aggtgattta ataccatagc acttaaaatg atggcgtaaa
     3661 taagtctcct gttgcttaga aacttctttg tcacttgcag ctttaaattt cctctctaaa
     3721 cttaggaata aatccattac gtaatcccct tgtatttttc aaaaagcatt gtaattcctt
     3781 catgtacaga ttctcttgat acacgtaaat tagcgtaagg agtcagttca tcaaaatccc
     3841 ctacttgaac actataatgc gcagcatcaa acatagaaat atcattttta tcattgccaa
     3901 aagctataaa acgattccca aataatttct tagtggcttt tcccttgtca actgcaatgg
     3961 gatttatgta caaacatttt tcaaattcgt ggaaaaagtg actactaagt ccaaaacgat
     4021 ttaatttcgc taacatttgg tctgcccttc tcagctgatc accaaaatac aacaccattt
     4081 ttatcggttt ctcaatttta gaaacttcta gaagctctcc ccttttttga ggatcaacat
     4141 aagcaataaa gggaatttta gaagcctgat aagtagcgta attaaagacc tcatcaacaa
     4201 aatatggaat ctgatagcga tgacaatatg aaataatagt actaaaaaag tcactttgta
     4261 aatagtaact atccactaag tgaccatttt catgtaaagt cgcaccattt aaaccaataa
     4321 tttttgataa agatagcttg tcgccaagga tacctatagt gtctctataa gatcgcgccg
     4381 tagcaaaagt gactctatga ccataatcaa tctgggctct ctctagtatt ccttgtattt
     4441 ctttagatag actcatacca tcaaaacaaa gagttccatc tatatcaaag acaaaatcca
     4501 ttctctcaaa gccccatcct taagttaaaa taggttttca tacgtgcata aacatcttca
     4561 tcaccaacaa aaaagatatg atctccttgc tctataactg agaaaggccc tggagaaaca
     4621 ataaatttcc cttcgtgttc aatagccaca atagtcgcac cagtttgatg ccatacattt
     4681 aattctccta ttgacttacc aaggtgttca gaatcttcac tgacaataat ttcatatggt
     4741 gctagtgggt attgtttact aaccgcacgt gtttgaagca aaaaatcatc aactaaagta
     4801 gctaactctt ccatttcttg ttgctgagcc ttaatattat cacggatttt acctttcaaa
     4861 atagcaactg aatgagaggt ttcatattgg tttaagaatg caattgcttt ttctttagaa
     4921 agaataatgg ctccactgcc atgctttaaa gttaaaatct gtaaatctgc taaaatatta
     4981 agacccttac gtgctgtttc tggtgatacg ttgaatgttg aagcaatagt tgttctagat
     5041 ttcaacttct ctccaacttc ataatctcca tttgcgattc tttgtgcaac cgctactgct
     5101 attttttgat atttagatgt tacaatttca gattgttcag aaaccatcca accctcctta
     5161 tcctaataaa aacatacata ttaaaccaca attagcaaaa tattgcaaat atatcttttt
     5221 tgttaatatc taatgtcttt agtaaatatc atataaaagg agctggtctc ccagctccga
     5281 gtgtcttatc ataaaaagta gtttactgaa ttaagattca ctagtacgag cttctgagat
     5341 tttttctttt gcagcctcag taccatttga tactgcttct tttgttttct ctgctccaga
     5401 agaaattgct gctttagctt tgccagcttg ttcagaagca aagtttcctg ttgcttgtgc
     5461 tgcatcagat acacgatctt gtactgtcac tgagtcagct tcatgttcag ctttagtctt
     5521 aacatctacg acatttgcgt tcaattcaac aactttaagg tgggtcatca cttcaacgtt
     5581 ttgactgaca atcgctttaa tgctttcaac gatagctggg atatccttgc cgtattcaac
     5641 aacgatgtct aaatcaactg caacttcttt agtaccaact tcaacattta caccatcagt
     5701 tactgagtca gaatttacaa cgctattttt tagattagag aagaaacctc cactcacagc
     5761 caagagtccg tctactgatt ctaaagcatg tccaacaatt ttagcaatta ccttttcttc
     5821 ataagttact gaagttttaa tatcttgttt ttgattttcc ataaataaat gacctcaaac
     5881 cttctagtcc tttttcttaa agctctctga agcaccctta atggtatctt taatgtcagc
     5941 aagtttttct tttgcatgac ctttcaattt atcgacttta ccttcagatt caacttcttt
     6001 atcgccagta agtttcccaa cagattcttt tactgaacca gaaaccttat caacttttgc
     6061 atcaaatttt tcttcagaca taatagcctc ctactaataa caatacttta aactatactt
     6121 aaatcaatta ttgtacacgt ggttcagact tcatgtcttc cactttatca acaccgttac
     6181 ctacggcact tttaacgttt gaaacttgat tcgaagcaaa ttcacctgtt gcttgtgctg
     6241 cacttgttac acgatcttga agagttactg aatcttcttc atgttgagca cgagttttaa
     6301 tatcaacaac attaacatta acttcaacca cttcaaggtc tgtcatacgt ttcacttcag
     6361 cttcaacaac ttttttgata tcggcaaaga tagttggaac atgtttttgg tattcagcaa
     6421 caatatccaa atcaacagca acttgctttt tacccacttc aacatttacg ccatctgtaa
     6481 ctgaatcaga atttacaacg ctatttttta aatttgagaa aaaaccacca tttacagcca
     6541 aaagaccatc tacatgctca atcgcaattc caacaatttt ttcaatcact ttatcttcga
     6601 aagttaattc tccacgaaca gctgtcgtgc cagaattgtt agttgtgttt ttgatgtaag
     6661 tttcagtcat aatagttcct ctttctttta tttaaataat gtagaataaa tataaacgaa
     6721 aaattttatt ttctcttatt aaagaattgg tcaagcatac ctgttctttg cacataaagc
     6781 ccagcataag cacctagcac aatgataaca agagcaagaa ttgttttaaa gaatccaatg
     6841 gttacaatca ttgcagctaa aacgagccca ataactgcac ctcctagtgg atatctgtat
     6901 ttcctcacga attctgacat attagactcc tattctacac gacttttatt cgtcttagca
     6961 tttccaacat tttcttcttc aacttgctta acaaatactc tagtgttcat tttcttttcc
     7021 aacccaaaga attcttttaa gccaagttca acatcacttt gaatatgttc aagttgttcc
     7081 ggtacatttg tacgagaagt taaaagacct ttaacatcaa ctttcacttt tttcttatat
     7141 aaatgagctg tcacactagg agatttcatc aaaccagttt ttaatacttc tgttttaaca
     7201 aatccctcta ttgcagattt ttttaacatc aatttaccag ttttatcagc cagtttatac
     7261 tctgtgtatc gtcttggata aaacagaact accaatatag ccagtaaaac gattacaaac
     7321 agcactaatg ctccccaaaa gaaataatga tacataccaa cgtcaatcgg tgatggcaaa
     7381 ttaaaatccc aatctaacca attaaaagat ggtagataaa tgtgttgttt actgataaca
     7441 gctacaaatc ctagtagggt aagggagatt agacctagca aagtatataa actttttagt
     7501 ccttttgaca taggtaactc cttttctagt tattcatttt acctagtaca aatgaagtaa
     7561 caatgacaac aataatcgca cctacgattg aaggaatcaa agccatacct gctaatttag
     7621 gcccccatgt tcctagtaaa ctttgaccaa caaatgaacc aactaaacca gctaagatgt
     7681 tagctatcca acccattgaa ccacctttgt tagtaacagc ccctgcaata gcaccgataa
     7741 tagcacctac gattaatgac caaattaaac tcatgataaa taccttctct ttcttgacta
     7801 atcattctac tagttatgta gttatgattt tttgcctaat acaaatgaag taacaatgat
     7861 aacgataatc gcacctacga ttgaaggaat caaagccata tctgctaact taggccccca
     7921 tgttcctagt aaactttgac caacaaatga accaactaaa ccagctaaga tgttagctat
     7981 ccaacccatt gaaccacctt tgttagtgat tgctcctgca atagcaccaa taatagcacc
     8041 aacgattaat gaccaaatta accccatgat aaataccttt cttcctaagt acatctcagt
     8101 gtacaaactt attttgttct taactaaata acaagagttt ctaaagtgac cactcggcaa
     8161 ttaattaagt tgttaactat actaatatta tacaacttta
//
LOCUS       DATROQ010000081         7982 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.082, whole genome shotgun sequence.
ACCESSION   DATROQ010000081 DATROQ010000000
VERSION     DATROQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.082"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(121..936)
                     /locus_tag="VBC94_001642"
     CDS             complement(121..936)
                     /locus_tag="VBC94_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6979900.1"
                     /translation="MTGSKKLAKSDYTKTALRAFYLQNGFNYSNYQGLGYANVIYPAL
                     KKYYGDDKKALAGALEENVEFYNTNLHFLPFVTSLHLAMLDNERPEEEIRGIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLATDGLVMGPILFFVAMNTILTGIKLVTGMYGYR
                     LGTSFIDKLSEQMSVISRAANIVGVTVISSLAATQVKLTIPYTFAPEKVTSTAQKIVT
                     VQGMLDKIAPALLPALYTFLMFYLIKNKKWTTYKLVILTVIIGILGSWLGILA"
     gene            complement(923..1789)
                     /locus_tag="VBC94_001643"
     CDS             complement(923..1789)
                     /locus_tag="VBC94_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO6979901.1"
                     /translation="MDISILQAVLIGLWTAFCFSGMLLGLYTNRCIVLSLGVGVILGD
                     IQTALAVGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITTAGTKGKITPEAALA
                     LSTPIAVGIQFLQTATYTAFAGAPETAKKALQAGNFRGFKIAANGTIWAFAGLGFGLG
                     VLGALSTQTLTDLFALIPPVLLNGLTLAGKMLPAIGFAMILSVMAKKELIPYILLGYV
                     LAVYFGLPVLTPTANGDGVLTSVATNSVLGVPTIGVAIIATIFALLDIFRKPAAPTKE
                     TKTEGDNQDDWI"
     gene            complement(1825..2319)
                     /locus_tag="VBC94_001644"
     CDS             complement(1825..2319)
                     /locus_tag="VBC94_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEO6979902.1"
                     /translation="MAAGPNIVMTRVDERLIHGQGQLWVKFLSCNTVIVANDDVSKDH
                     LQQTLMKTVVPESIALRFFDIQKVIDIIHKANPAQTIFIIVKDLKDVYRLVAGGVPIK
                     EINIGNIHNGEGKEQVSRSIFLGMKDKEIIRKLNQEYHIAFNTKTTPTGNDGAVEVNI
                     LDYI"
     gene            complement(2374..3570)
                     /locus_tag="VBC94_001645"
     CDS             complement(2374..3570)
                     /locus_tag="VBC94_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEO6979903.1"
                     /translation="MMKIKPVKVESIENPKRFLDSRLLTKIEVEEAIEKALKQLYINI
                     DYFGEEYPTPATFNNTYKVMDNTEWTNGFWTGCLWLAYEYNQDKKLKNIAHKNVLSFL
                     NRINNRIALDHHDLGFLYTPSCTAEYRINGDAKALEATIKAADKLMERYQEKGGFIQA
                     WGELGYKEHYRLIIDCLLNIQLLFFAYEQTGDEKYRQVAVNHFYASANNVVRDDSSAF
                     HTFYFDPETGEPLKGVTRQGYSDESSWARGQAWGIYGIPLSYRKMKDYQQIILFKGMT
                     NYFLNRLPEDKVSYWDLIFTDGSGQPRDTSATATAVCGIHEMLKHLPEVDPDKETYKY
                     AMHTMLRSLIEQYSNSEFIAGRPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYK
                     DWELYW"
     gene            complement(3573..4007)
                     /locus_tag="VBC94_001646"
     CDS             complement(3573..4007)
                     /locus_tag="VBC94_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6979904.1"
                     /translation="MIKIIIVAHGNFPDGILSSLELIAGHQEYVVGINFIAGMSSNDV
                     RVALQREVIDFKEILVLTDLLGGTPFNVSSALSVEYTDKKIKVLSGLNLSMLMEAVLS
                     RTMFEHVDDLVDKVITSSHEGIVDFSTCLATQTAEATFEGGI"
     gene            4285..5097
                     /gene="dhuD"
                     /locus_tag="VBC94_001647"
     CDS             4285..5097
                     /gene="dhuD"
                     /locus_tag="VBC94_001647"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric 2-dehydro-3-deoxy-D-gluconate
                     5-dehydrogenase DhuD"
                     /protein_id="HEO6979905.1"
                     /translation="MTEQFLKDNFSLEGKVALITGASYGIGFSIATAFARAGATIVFN
                     DIKQELVDKGISAYKKLGIKAHGYVCDVTDEDGINEMVNKISQDVGVIDILVNNAGII
                     KRTPMLEMSAADFRQVIDIDLNAPFIVSKAVLPGMIQKGHGKIINICSMMSELGRETV
                     AAYAAAKGGLKMLTKNIASEYGSANIQCNGIGPGYIATPQTAPLRERQDDGSRHPFDQ
                     FIIAKTPAARWGEAEDLGAPAIFLASDASNFINGHILYVDGGILAYIGKQPQ"
     gene            5114..5752
                     /gene="dhuI"
                     /locus_tag="VBC94_001648"
     CDS             5114..5752
                     /gene="dhuI"
                     /locus_tag="VBC94_001648"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase DhuI"
                     /protein_id="HEO6979906.1"
                     /translation="MKIALINENSQASKNTLIYKELKAVSKKKGFDVFNYGMYGKEEE
                     SQLTYVQNGLLTAILLNSGAADFVITGCGTGIGAMLACNSFPGVVCGFAADSVDAYLF
                     SQVNGGNALSLPFAKGFGWGAELNLRYLFERLFEDEKGGGYPKERAVPEQRNARILSE
                     VKQITYRDLLSVLKEIDQDFLKETISGEHFQEYFFANCQNQNIADYLKSVLD"
     gene            5778..6785
                     /locus_tag="VBC94_001649"
     CDS             5778..6785
                     /locus_tag="VBC94_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO6979907.1"
                     /translation="MSKILFFGEPLIRITPKENDYFADSISTKLFYGGSEVNIARALQ
                     GFGQDTKLLSALPNNPIGNSFLQFLKAQGIDTHSIQWVGERVGLYFLENSFACRKGEV
                     VYDRDYSSLHDFRINQIDFDQLFEGVSLFHFSGITLSLDESIQEITLLLLKEAKKREI
                     TISLDLNFRSKLISPKNAKILFSQFATFADICFGIEPLMVDSQDTTFFNRDEATIEDI
                     KERMISLINHFDFQVIFHTKRLQDEWGRNHYQAYIANRKQEFVTSKEITTAVNQRIGS
                     GDAFVAGALYQLLQHSDSKTVIDFAVASASLKCALEGDNMFETVTAVNKVLNQSKDII
                     R"
     gene            6797..7435
                     /locus_tag="VBC94_001650"
     CDS             6797..7435
                     /locus_tag="VBC94_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO6979908.1"
                     /translation="MIKSKILLQLMQGKIVAVIRGNSQEEAFQAAQACIKGGISAIEI
                     AYTNSKASQVIEQLVTQYTNQEQVVVGAGTVLDAETARMAILAGAKFIVSPAFNLQTA
                     KLCNRYAIPYLPGCMTLSEITTALEAGCEIIKIFPGGTLGTSFISSLKAPLPQVQIMV
                     TGGVNLTNAKDWFLSGVTAIGIGGEFNKLAALGEFDKITEMAKQYKIQLNLN"
BASE COUNT         2531 a         1558 c         1372 g         2521 t
ORIGIN      
        1 ctatcaaaat ccttgaattt attatataag cgatctttca tttttttaca aaacgtcctc
       61 gtccgatata aaaaggacta tgtcattgat atatcataat ccttatttat caaaatttta
      121 ttaagctaaa atacctaacc agctaccaag gataccaata ataaccgtaa ggataactag
      181 cttataagtt gtccattttt tatttttaat aaggtaaaac ataaggaaag tgtagagtgc
      241 tggaagtaga gctggtgcaa ttttatcaag cataccttgg acagtaacaa ttttttgcgc
      301 tgtactagtt actttttctg gagcaaaagt gtaagggatt gtaagcttaa cttgtgttgc
      361 agctaaactt gagatgaccg ttacaccaac gatattagct gcccgtgaga taactgacat
      421 ttgttcactc aatttatcaa taaaactggt cccaagacga taaccataca tgccagtaac
      481 taatttaatt cccgttaaaa tagtattcat agccacaaag aacagaatcg gtcccatcac
      541 caaaccatct gtcgctaaag aggctgcaat cgttgagaac aagggtgcta agcaaaattg
      601 tgataaagaa tcaccgatac ctgcaagagg acccataagc gccatcttaa taccgcgaat
      661 ttcttcttct ggccgctcat tatcaagcat tgctaaatgt aaactagtta caaaaggtaa
      721 gaaatgaagg tttgtattgt aaaactccac attttcttct agagctcccg ctaaagcttt
      781 tttgtcatct ccataatatt ttttgagagc tggatatatg acattagcat aaccaagacc
      841 ttgatagtta ctgtaattaa agccattttg taaataaaag gcacgtaaag ctgttttagt
      901 gtaatcactt tttgctaatt ttttagatcc agtcatcttg gttgtctcct tctgtcttag
      961 tttctttagt aggtgcagct ggttttctga aaatatcgag caaagcaaat attgttgcaa
     1021 ttatagctac cccgatagtt ggaaccccaa gcacactgtt agtagccact gatgttaaaa
     1081 ctccgtctcc atttgctgtt ggagttagaa ctggcaaacc aaaataaaca gctagaacat
     1141 atccaagtag gatataagga atgagttctt ttttagccat tacagagaga atcatcgcaa
     1201 aaccaattgc aggaagcatt ttcccagcta aggttagacc attaagcaaa acagggggaa
     1261 tgagagcaaa taaatctgtc aaagtttgcg ttgaaagagc acccagtact cctaaaccaa
     1321 atcccaatcc agcgaatgcc caaattgtac catttgctgc aattttaaat cctcggaaat
     1381 tacctgcttg caaagctttt ttagctgttt caggcgcacc tgcaaaagca gtataagttg
     1441 ctgtttgaag aaattgaata ccaactgcaa ttggggttga taatgcaaga gcagcttccg
     1501 gtgtgatttt tcctttagtt ccagctgttg taatagccat caaagttcca aaaatcccag
     1561 gaccgattgg gtttggagga actgtccctc ccgctcccac accaaatccc atgtaagcca
     1621 gttcagatat tgctccaact gccaaagctg tttgaatatc tcctaaaata acaccaacgc
     1681 ctagagagag aacgatacaa cgatttgtat agaggccaag caacatgcca ctaaaacaga
     1741 aagccgtcca cagtccaatt aagacagcct gaagaatact aatatccata tttttctcct
     1801 ttatatgata gtgagtttgt ccaactaaat ataatctaaa atattaactt ccacagcacc
     1861 atcgttacct gttggagttg ttttagtatt aaatgcaatg tgatattctt ggtttaattt
     1921 tctaattatc tctttatcct tcatccctaa gaagatagag cgacttacct gctctttacc
     1981 ttctccatta tgaatatttc caatattaat ctctttaatt gggacgcctc ctgctacaag
     2041 tctatagaca tctttcaaat ctttaacaat tataaaaatc gtctgtgctg ggttggcctt
     2101 gtgaataata tcaataactt tttgaatatc aaaaaatcgc aatgcaattg attctggcac
     2161 caccgtcttc attaacgttt gttgaagatg atctttggag acatcatcat ttgccacaat
     2221 aacagtatta caacttaaaa atttcaccca aagttgtcct tgtccatgaa taagacgctc
     2281 atcaacacgt gtcattacaa tatttggtcc tgctgccatt tctgacctcc ttgttttcta
     2341 ttatttattg tttattttgt tagtaaaata ctcttaccaa taaagttccc agtctttata
     2401 gaatcttatt aaggcttcta agtaataata atctccccaa atattacctt catctactcc
     2461 tttacctgaa tgccacgaat acacaccgtg caatagaaga ggacgtcctg ctataaattc
     2521 actattacta tactgttcaa tcagactacg aagcattgta tgcatagcat atttgtatgt
     2581 ctctttatca ggatctactt ctggtaaatg tttaagcatc tcatgaatcc cacacacagc
     2641 tgttgctgtt gcggatgtat ctctaggctg acccgagcca tccgtaaaaa taaggtccca
     2701 ataggatacc ttgtcttcag gtaaacgatt tagaaaatag tttgtcatac ctttaaaaag
     2761 gataatctgc tgataatctt tcattttccg gtaactaagc ggaataccgt agatgcccca
     2821 tgcttgccct cttgcccaag atgactcatc actataaccc tgtcgtgtga caccttttaa
     2881 cggttctcca gtttctgggt cgaaataaaa agtatgaaaa gcagaagaat catcacgcac
     2941 cacattgtta gctgaagcgt agaagtgatt caccgcaact tgtctatact tttcatcacc
     3001 tgtctgttca taagcaaaaa ataagagttg gatattaagt aagcaatcga taattaagcg
     3061 atagtgttcc ttatacccga gttctcccca agcctgaata aatccacctt tttcttgata
     3121 gcgctccatc aatttatctg cagcttttat agtggcttct aaagctttag catcaccatt
     3181 gatacgatat tctgctgtac aagatggtgt gtaaagaaat cctaagtcgt ggtgatctaa
     3241 tgctatacga ttattaatac gatttagaaa tgacaataca tttttgtggg ctatgttttt
     3301 taacttttta tcctgattat actcataagc taaccacaag caccctgtcc aaaaaccatt
     3361 tgtccattct gtgttatcca taactttata agtattattg aatgttgcag gcgttggata
     3421 ctcttcacca aagtaatcaa tattaatata aagttgcttc aaggcttttt caatggcttc
     3481 ctcaacttca atcttagtta ataatctact gtctaaaaaa cgctttggat tttcaattga
     3541 ctcaaccttg accggtttta ttttcatcat tattaaatgc ctccctcaaa agttgcttct
     3601 gcagtttgtg tagcaaggca agtagaaaaa tcaacaattc cttcatgaga tgatgttata
     3661 actttatcaa caaggtcatc tacatgttca aacatcgttc tagataaaac agcttccatt
     3721 aacattgaaa ggttcaagcc cgataaaact tttatctttt tatctgtata ttctactgat
     3781 aaggcggaag aaacattgaa aggagtccca cctagcaaat cagtcaatac taaaatttct
     3841 ttgaaatcga taacctctct ttgcaaagca actctaacat cattagaaga catgcctgca
     3901 ataaagttaa taccaacaac atattcctga tgcccagcaa taagttctaa agagcttaaa
     3961 ataccatctg gaaagttgcc gtgtgctaca ataataattt ttatcatatt ttctcctctt
     4021 gagtttaccg gtttactttt ataatcatat tatagcgctt tcatttttag gtgtcaacat
     4081 cttttttata ttttttgagc agtttacttg attttttata atgatgtctt ttttatcttt
     4141 aagaatcata acttagataa agctttgtta tatatgttgc caagttttat aggagaagaa
     4201 ctctatagtt gacaaaatcg gtttactttg ttactattca tgtaaacagg tttacttcct
     4261 gaattttttt acggaggtca tattatgact gaacaattct taaaagacaa cttttcacta
     4321 gaaggtaaag tcgccttaat aacaggcgct tcttacggca ttggtttttc aatagcaact
     4381 gcttttgcta gagctggagc aactattgtc ttcaatgata ttaagcagga actcgttgac
     4441 aaaggcatct ccgcctataa aaaacttggc attaaggccc atggctatgt ttgcgatgtt
     4501 acagatgaag atggaatcaa cgaaatggtg aacaaaattt ctcaagatgt tggggtaatt
     4561 gatattctag ttaacaacgc aggtattata aaacgcaccc ctatgctaga gatgagcgct
     4621 gcagattttc gacaagttat tgatattgat ttgaatgctc cctttatcgt atcaaaagcg
     4681 gtattacctg gaatgatcca aaaaggacat ggtaaaatca tcaatatttg ctccatgatg
     4741 agtgagcttg gacgcgaaac agttgctgcc tatgctgctg ccaaaggggg acttaaaatg
     4801 ttaaccaaga acattgcttc tgaatacggt tctgctaaca tccaatgtaa cggtatcggg
     4861 cccggctata ttgccacacc acaaacagct ccccttcgtg aacgtcaaga tgatggttca
     4921 cgtcatccat tcgaccaatt tatcatcgct aaaacacctg ccgctcgctg gggtgaggca
     4981 gaagatttag gtgcaccagc tatcttcctt gctagcgatg catcaaactt tatcaatggt
     5041 cacatactct atgtagatgg cggtatttta gcttacatcg gtaaacaacc tcaataataa
     5101 aggagtaata taaatgaaaa ttgcattaat caacgaaaat agccaagcga gtaaaaatac
     5161 tcttatctac aaagaattga aagcggtttc caaaaaaaaa ggatttgatg ttttcaacta
     5221 cgggatgtat ggcaaagaag aagaaagcca gctaacttat gtacaaaatg gtttactgac
     5281 tgctatttta cttaatagcg gtgctgcaga ttttgttatt acaggctgtg ggacaggaat
     5341 tggagcgatg cttgcttgta atagctttcc tggcgtagtc tgtggattcg ctgctgattc
     5401 tgtcgacgct tacttattct ctcaagtaaa cggaggaaat gctctttccc ttccttttgc
     5461 taaaggattt ggttggggag ctgaattgaa tctgaggtat ttatttgaac gtcttttcga
     5521 agatgaaaaa ggagggggat accctaaaga gagggctgta cctgagcaac gcaatgcacg
     5581 tatccttagc gaagtaaaac agattactta cagagattta ctctctgttt taaaagaaat
     5641 tgatcaagat ttcttaaagg aaactatttc tggggagcat ttccaagaat atttcttcgc
     5701 taattgccaa aatcaaaata tagcagacta cttgaaatct gtcttagact aaagcgtctg
     5761 ttttagaaag gacttttatg tctaagattc tttttttcgg tgaacctctc attcgcatca
     5821 ctcccaaaga aaatgactat tttgcagata gtatctcaac taaactattc tatggtggct
     5881 ctgaagttaa tatcgcaaga gctctacaag gctttgggca agatactaaa ctcctctcag
     5941 ccttacccaa taatcccata ggtaatagtt ttcttcaatt tttaaaagca cagggtattg
     6001 atactcattc tattcagtgg gttggagaac gtgttgggtt atatttctta gaaaattcct
     6061 ttgcttgcag aaaaggtgag gtggtctacg accgcgatta tagtagttta catgacttta
     6121 ggataaacca aattgatttc gatcaattat ttgaaggagt ttctctcttt cattttagtg
     6181 gtattacact atcattggat gagtctatcc aagagataac actgcttctt ttaaaagaag
     6241 ctaaaaaaag agagataaca atctccttag atctcaattt tcgcagtaaa ctaatttctc
     6301 ctaaaaatgc caaaatcctt ttttctcagt tcgctacctt tgcggatatt tgttttggta
     6361 ttgaacctct tatggttgac tcacaggaca ctaccttctt caatcgcgat gaagctacaa
     6421 tagaggacat caaagaacga atgatttctc ttataaatca ctttgacttt caagtcattt
     6481 tccatactaa acgtctacag gatgaatggg gacgaaacca ctaccaagct tacatagcaa
     6541 atcgtaaaca agagtttgtc acttcaaaag aaatcaccac agctgtaaac cagagaattg
     6601 gaagcggaga tgcttttgtg gcgggagcgc tatatcaact acttcagcac tcagacagca
     6661 agacagtcat tgatttcgct gtcgctagtg catctttaaa atgtgctttg gaaggggaca
     6721 atatgtttga gaccgttacc gcagttaaca aagtactaaa tcaatcgaaa gacattattc
     6781 gttagaaggg agaattatga taaaatcaaa gattttgttg caattaatgc aaggaaaaat
     6841 agttgctgtc attcgtggaa atagccaaga agaagctttt caagctgctc aagcatgcat
     6901 aaaaggtggt atttctgcta tcgaaatcgc ctatactaat agcaaagcta gccaagtcat
     6961 tgaacaacta gtcacacaat atactaacca agaacaagtt gtcgttgggg caggaactgt
     7021 tctagacgca gaaactgccc gaatggctat tttagcagga gcaaagttta ttgtatctcc
     7081 cgcatttaat ctacagactg ctaaattatg taatcgttat gctattcctt atttaccagg
     7141 ttgcatgacc ttatcagaga taacaacagc acttgaagct ggttgtgaaa taattaaaat
     7201 attcccagga ggtactctag gaacatcttt catctcatca ctaaaagcac ctttacctca
     7261 agtacaaatt atggtgactg gtggggtaaa tctcacaaat gccaaagact ggtttctatc
     7321 tggagtaaca gccattggta ttggtggaga gttcaataaa ctagcagctt tgggggaatt
     7381 tgataaaatc acagaaatgg ctaaacaata caaaatacag ctaaatctga attaacacaa
     7441 taaaaagaca aggcaccccc acacctcaat catgagacag aagaaaagaa cttttataaa
     7501 gccatttttc tgtagtaccg tttatcttaa tcagactcat tatctcgatt ttcttatcag
     7561 accgagataa tttttagcta atcttcctgt agacataacc gttctacttc cgacaactga
     7621 tccatgtctt caccaaagat atactacttg cccgcagatt gatataagta gtgaagctca
     7681 tcattctcat atcatttcag caaataatag atttgatatc atttttatat ccaaaaactt
     7741 agcaatgacc tatcaggctt acccgctttt cctatctcaa cacaggctaa cttagctcct
     7801 atcaaataca ttttttaaac ataattaaaa acatttctga ttatagttta ttagatttca
     7861 acagaagtat ttttcctgcg tctcagcttt tgggtgtacc gttattttga agttttgata
     7921 gttgagtgat atcattaata gtcatttttt agacattcta aacctccttt ctaatttgac
     7981 cg
//
LOCUS       DATROQ010000082         7886 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.083, whole genome shotgun sequence.
ACCESSION   DATROQ010000082 DATROQ010000000
VERSION     DATROQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7886
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.083"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..677
                     /locus_tag="VBC94_001651"
     CDS             <1..677
                     /locus_tag="VBC94_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEO6979909.1"
                     /translation="FCSPHNPGGRVWTKGEIQKIGDICKRYNVILVSDEIHQDLVLFD
                     NVHHSFNTVDSSFKELSVILSSATKTFNIAGTKNSFAIIENEKLRSDFKKRQIANNQQ
                     EISSLGLLATEVAFTKGKQWLKALKMELEGSIEYLYEQLTQKTHIKVMKPEGTYLVWL
                     DFSAYNLTHLEIQEKLRYDAKLILNDGLTFGKEGKKHARINVAAPRSVIEEAVLRLVT
                     NFRKND"
     gene            787..1416
                     /gene="upp"
                     /locus_tag="VBC94_001652"
     CDS             787..1416
                     /gene="upp"
                     /locus_tag="VBC94_001652"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEO6979910.1"
                     /translation="MGKFQVISHPLIQHKLSILRRTTTSTKDFRELVDEIAMLMGYEV
                     SRDLPLEDVEIQTPVATTVQKQLAGKKLAIVPILRAGIGMVDGFLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGAASIKFVCL
                     VAAPEGVAALQEAHPDVDIYTAALDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            1541..2131
                     /locus_tag="VBC94_001653"
     CDS             1541..2131
                     /locus_tag="VBC94_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEO6979911.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGQVEDNMANSIIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKSDVQTIVMGMAASMGTIIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQSDMAIAAEHLLKTRHTLEKILADNSGQ
                     SIEKVHDDAERDRWMSAQETLDYGFIDAIMENNNLQ"
     gene            2231..2476
                     /locus_tag="VBC94_001654"
     CDS             2231..2476
                     /locus_tag="VBC94_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2129 domain-containing protein"
                     /protein_id="HEO6979912.1"
                     /translation="MNKQERLGMSVYLYYNRDARKLYKYGDVHYHSRRLRYLVIYVNK
                     EDIVSVSKEIKHLKFVKDVRLSAIDDIDQDFVGNLYR"
     gene            2631..3797
                     /locus_tag="VBC94_001655"
     CDS             2631..3797
                     /locus_tag="VBC94_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6979913.1"
                     /translation="MEKRLSLGALVLASTVLLAACGNVGGGASSTGTKIGKDIKVGYN
                     WELSGNVSSYGNSMKNGADLAVKEINAAGGVGGKKLKVLSQDNKSENAEAATVATNLV
                     TKGANVIIGPATSGAAASSTPKVNAAAVPMIAPAATQDNLVYGSDGKTLNQYFFRATF
                     VDNYQGKLLSQYATDNLKAKKVVLFYDNSSDYSKGVAKSFKESYSGKIVDSMTFSAGD
                     TDFQASLTKLKGKEYDAIVMPGYYTETGLIVKQARDLGISKPVLGPDGFDSPKFVQSA
                     TPVGASNVYYLTGFTTQGSTKAKAFHDHYVKAYGEEPSMFSALSYDAVYMAAKSAKGA
                     KTSIDIKKALAKLKDFKGVTGKMSIDKNHNVVKSAYVVKLEDGKTSSVNIISAK"
     gene            3903..4772
                     /locus_tag="VBC94_001656"
     CDS             3903..4772
                     /locus_tag="VBC94_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO6979914.1"
                     /translation="MLQQLVNGLILGSIYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FMGYYLINHLHLNFFLALLIAMLGSAFLGVVIEYLAYRPLRKSTRIAALITAIGVSFL
                     LEYGMVYLVGADTRAFPQAIHTVKYNLGPITITNVQLIILGIALLLMLTLQFIVQKTK
                     MGKAMRALSVDSDAAQLMGINVNRTISFTFALGSALAGAGGVLIGLYYNSVQPLMGVT
                     PGLKAFVAAVLGGIGIIPGAAIGGFVIGILETLATALGVSDFRDGIVYAILILIFLIR
                     PAGILGKNIKEKV"
     gene            4775..5728
                     /locus_tag="VBC94_001657"
     CDS             4775..5728
                     /locus_tag="VBC94_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO6979915.1"
                     /translation="MKENMKSILSWLAIVVLGYLLISVLISMGIFNLYHIQIIETIGI
                     NVILAVGLNLIVGCSGQFSLGHAGFMAIGAYAVAIIGVKMPTYVGFLIAILVGTLVAG
                     GIALGVGIPTLRLKGDYLAIATLGVAEIIRILLVNGGDITNGAAGIMGIPPFTTWSLV
                     YGVAVVSLILAMNFLRSPLGRNTIAIREDEIAAESMGVDTTKVKVIVFVFGAILASIA
                     GSLQAGYVGTVMPKDFSFMMSVNVLIIVVLGGLGSMTGTVLAAILLGLLNMLLQDYAS
                     VRMIIYALALILIMIFRPSGLLGTKELTLSHLFRKTSKGGN"
     gene            5729..6553
                     /locus_tag="VBC94_001658"
     CDS             5729..6553
                     /locus_tag="VBC94_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979916.1"
                     /translation="MALLEVKNLSKHFGGLTAVGDVSMKLHKGELIGLIGPNGAGKTT
                     LFNLLTGVYLPSKGTISLDGKILNGRKPAKIASLGLGRTFQNIRLFKNMTVLDNVLVG
                     LSNHHLSHPIASFLRLPKYYHSEKALRKKALELLEIFGLKAYQDALAKNLPYGKQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTQLISQIKSDFDITIMLIEHDMNLVMQV
                     TERIYVLEYGRLIAHGTPEEIKNNKRVIEAYLGGSYNDHVESRKSIYSLWCYSSGE"
     gene            6492..7202
                     /locus_tag="VBC94_001659"
     CDS             6492..7202
                     /locus_tag="VBC94_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979917.1"
                     /translation="MTMLKVENLSIHYGVIQAVNDVSFEVNQGEVVTLIGANGAGKTS
                     ILRTISGLVRPSQGSISFMGKPIHKLAARKIVGNGLAQVPEGRHVFSSLSVMENLEMG
                     AFLQKDREQNQKMLKKVFDRFPRLEERKNQDAATLSGGEQQMLAMGRALMSRPKLLLL
                     DEQSMGLAPIFIQEIFNIIEDIKKQGTTVLLVEQNANKALTIADKAYVLETGKVVLSG
                     TGKELLVSDQVRKAYLGG"
     gene            7221..7880
                     /locus_tag="VBC94_001660"
     CDS             7221..7880
                     /locus_tag="VBC94_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6979918.1"
                     /translation="MPVKDFMTKKLVYVSPDTTVAEAADLLREHHLRRLPVVENDQLV
                     GLVTEGTMAEAQPSKATSLSIYEMNYLLNKTKIRDIMIKDIVTVSQYASLEDAIYLMM
                     SRKIGVLPVVDNGQLYGIVTDRDVFKAFLEIAGYGQEGYRLVILADEGIGVLSKVLNR
                     LSSANLSVKRLVIIERKAGKKAVEIQLEGYADKDVLKQELVFDDVIVETLEKTMKKPL
                     S"
BASE COUNT         2523 a         1148 c         1668 g         2547 t
ORIGIN      
        1 ttttttgtag tccacacaat cccggtgggc gtgtctggac aaaaggagaa attcaaaaaa
       61 taggagatat ctgtaaacga tataatgtta tcctcgtttc tgacgaaata catcaagatt
      121 tggtattatt tgataatgta caccatagct ttaatactgt tgattctagt tttaaagaat
      181 tgagtgttat tttatctagt gcaactaaaa cgtttaatat tgcagggact aaaaatagtt
      241 ttgcaataat tgaaaatgaa aagttacgct cggattttaa gaagaggcaa atagctaaca
      301 atcaacaaga aatttcttca ttggggttat tagctacaga agttgccttt acaaaaggga
      361 aacaatggtt aaaggcccta aaaatggagt tagaaggatc aatagaatac ctttatgagc
      421 aattaacgca aaaaactcat atcaaagtga tgaaaccaga gggaacttat cttgtttggt
      481 tagatttttc tgcttataat ttaacgcatt tagaaattca agaaaaactt cgatatgatg
      541 ccaaattaat attaaatgat ggtttaactt ttggaaaaga agggaaaaaa catgctagaa
      601 tcaatgtcgc agctcctagg tctgttattg aggaggcagt tttgcgatta gtgacaaact
      661 ttagaaaaaa tgactagact ataaaaaaag tcgcatggaa agggttttta tgttataata
      721 ctttgaatgt aaacaagcat tagaatgttt ttaatgctca aaattaaata gaaagcggtc
      781 attataatgg gaaaatttca agtcatttca catccactta ttcaacataa actgtcaatc
      841 ttacgtcgca ctacaacatc tactaaagat tttcgtgagt tagttgatga gattgcgatg
      901 ttaatgggct atgaagtgtc tcgtgatttg cctttagaag atgttgaaat ccaaacacca
      961 gtagcgacaa ctgtacaaaa acaattagct gggaaaaagt tagcaattgt tccaatttta
     1021 cgtgcaggta ttggtatggt tgatggtttc ttaagccttg tgccagctgc taaagttggt
     1081 catatcggta tgtatcgtga tgaagagact ttccaaccag tcgaatattt agttaagtta
     1141 cctgaagata ttgatcaacg tcagattttt gtagttgatc caatgcttgc tacaggtggg
     1201 tctgctattt tagcagtaga ttctctaaaa aaacgtggcg cagctagtat caaattcgtt
     1261 tgtttagtag cagctccaga aggagtggca gcacttcaag aagcacatcc agatgtagat
     1321 atttataccg ctgctttgga tgaaaaactc aatgaacatg gctacattgt tcctggactc
     1381 ggagatgctg gggatcgctt atttggtaca aaataagatt tgagatgaat aaggcttttc
     1441 tgaagaactc agaaagcctt ttcttaaaaa agttattatt tgacctttgt tgaccaaatg
     1501 attataatag tgacagtgat aaaaagaaat ggagaaagat atgattccag tagttattga
     1561 acaaacaagt cgtggtgaac gttcttatga tatttactca cgtcttttaa aagatcgtat
     1621 tattatgttg acaggccaag ttgaggataa tatggccaat agtatcattg cacagttatt
     1681 gtttctcgat gcacaagata atacaaagga tatttacctt tatgtcaata caccaggtgg
     1741 ttcagtatcg gctggacttg ctattgtgga caccatgaac ttcattaaat cggacgtaca
     1801 gacgattgtt atggggatgg ctgcttcgat gggaaccatt attgcttcaa gtggtgctaa
     1861 aggaaaacgt tttatgttac cgaatgcaga atatatgatc caccaaccaa tgggcggaac
     1921 aggcggaggt acacagcaat ctgatatggc tatcgctgct gagcatcttt taaaaacgcg
     1981 tcatacttta gaaaaaatct tagctgataa ttctggtcaa tctattgaaa aagtccatga
     2041 tgatgcagag cgtgatcgtt ggatgagtgc tcaagaaaca cttgattatg gctttattga
     2101 tgctattatg gaaaataata atttacaata atagatttaa aagagttgag tttaccaact
     2161 ctttttttat ttgttggaat tatgttataa tcttagtaat tacagatatg acgcagaaag
     2221 gaaaaaatta ttgaataaac aagagcgttt aggaatgagc gtttatcttt actataatcg
     2281 tgacgctcgt aaattatata aatacggaga tgttcattat cattctcgta gattaagata
     2341 tctagtcatt tatgttaata aagaggatat tgttagcgtt agtaaagaga taaaacattt
     2401 aaaatttgtt aaggatgttc ggttatcagc aattgatgat attgatcagg attttgttgg
     2461 aaacttatat cgttaagata tttactagta gttattagaa agaaggagtt cagatgtcct
     2521 tcttttttat ttagtttcta taattttcaa aaaaacaatt taaaaactat tgacaatatt
     2581 ctgataattc tgtattattt tagttaccat tttcaaaaaa ggagaaaaat atggaaaaga
     2641 gacttagttt aggagcgctt gttttagcta gtacagttct tttagcagca tgtggaaatg
     2701 tcggaggagg agcatcttcg acaggaacaa aaatagggaa agatattaag gtagggtata
     2761 actgggaatt atcaggaaac gtttcttcat atggaaattc aatgaagaat ggagctgact
     2821 tagcagtaaa agaaattaat gctgctggag gggttggcgg caagaagtta aaagtcctat
     2881 cacaggataa taaatcagaa aatgcagagg cagcaacggt tgccacaaac ttggttacca
     2941 aaggagctaa tgtcattatc ggaccagcaa catcgggtgc agctgcatct tcaactccaa
     3001 aagtaaatgc agcagcagtt ccaatgattg cacctgctgc gacacaagac aatttagtct
     3061 atggttctga tggaaaaacc ttaaatcagt atttcttccg agctactttt gtcgataatt
     3121 atcaaggaaa gctattgtct cagtatgcta cagacaacct taaagctaaa aaagttgttc
     3181 tattttatga taattcatca gattactcaa agggggtagc aaaatcattt aaggaaagtt
     3241 atagtggaaa aattgttgat agtatgacat tctccgctgg tgatactgat ttccaagcgt
     3301 cattgactaa gttgaaaggg aaagaatatg atgctattgt gatgccaggt tactataccg
     3361 agacaggatt aatagttaag caagcgcgtg atttaggtat ctctaaaccg gttcttgggc
     3421 ctgatggttt tgatagtccg aaatttgtgc aatcggcaac acctgtagga gcttcaaacg
     3481 tttattattt gacaggtttc actacacaag gatcaaccaa agctaaagct ttccatgatc
     3541 attacgttaa ggcatatggt gaagaaccat ccatgttctc ggctttatca tacgacgctg
     3601 tgtatatggc agctaaatct gctaaaggag ctaaaacttc aattgatatt aaaaaagctt
     3661 tagctaagct gaaagatttt aaaggtgtga cagggaaaat gtctattgat aaaaatcaca
     3721 atgttgttaa atcagcttac gttgtcaaat tagaggatgg aaaaacaagt agcgttaaca
     3781 ttatttcagc aaaataagta ttctctagaa aaatgggagt ttaattttca gctccccttt
     3841 tttaaaaagt aatttttcag aataattcga caataatata taatagaaag tatggttata
     3901 atatgcttca acagctcgtt aatggactta tcttaggatc catatatgct ttacttgcct
     3961 taggatatac tatggtatat ggcattatta agctgattaa ttttgctcat ggggacatct
     4021 atatgatggg cgctttcatg ggctattatt tgattaatca tcttcatctt aattttttcc
     4081 tagctttatt aattgcaatg ctggggtctg ctttcctagg tgttgtaatt gaatatttag
     4141 cttatcgtcc actgagaaaa tcaactagaa ttgccgcgtt aattacagct attggtgttt
     4201 cttttctttt ggagtacggg atggtatacc ttgttggagc ggatactagg gcatttccgc
     4261 aagccattca tactgttaaa tataatttag gaccgataac tataaccaat gttcagctca
     4321 tcattttagg tattgcgcta ttactaatgc ttactttaca gttcatcgtt caaaaaacta
     4381 aaatggggaa agctatgaga gctttatcag tggatagcga tgccgcacaa ttgatgggaa
     4441 ttaatgttaa ccgtaccatt agttttacat ttgcgctggg gtctgcttta gctggagcag
     4501 gtggagtact gattgggctt tattataatt ctgttcaacc tcttatgggg gtaactccag
     4561 ggttgaaagc atttgttgca gctgtcttag gtggtattgg tatcattccg ggagcggcta
     4621 tcggtggttt tgttattggc attttagaaa ctttagcgac tgcacttggt gtgtcagatt
     4681 ttcgagatgg tatcgtttat gctattttaa ttctcatttt cttgatccgt ccagcaggaa
     4741 ttcttggaaa aaatattaag gagaaggtct aggcatgaaa gaaaatatga aatcaatttt
     4801 atcttggtta gctatagtag tgttaggtta tctcttgatt agcgtgctaa tatctatggg
     4861 tatatttaac ctctatcata ttcaaattat tgaaacaatt gggattaatg ttattttagc
     4921 agttggttta aacctaattg taggttgttc gggacagttt tcactaggac atgcaggttt
     4981 catggctatt ggcgcttatg cagttgctat tatcggcgtt aaaatgccaa cttatgtagg
     5041 ttttttgata gctatacttg ttggaactct tgttgcagga ggtattgcct taggtgtagg
     5101 aattcctact ttgagactaa aaggtgatta tttagctata gctactttag gtgttgctga
     5161 gattatccgt attttgttgg ttaatggagg tgatattacg aatggtgctg cgggtatcat
     5221 ggggattccg ccttttacaa cctggtcttt agtttatggg gtggcagttg tgagccttat
     5281 ccttgcaatg aattttttgc gtagtccctt agggcgtaat acaattgcta tacgagaaga
     5341 tgaaattgca gcagagtcta tgggtgtgga tacaacaaaa gtgaaagtaa ttgtttttgt
     5401 ttttggagct attttagctt ctatcgctgg tagtttacag gctggctacg ttggaactgt
     5461 tatgccaaaa gacttttcat ttatgatgtc tgtcaatgtt ttaattattg ttgttttagg
     5521 tggtttaggt tcaatgacag ggacagtttt agcagcaatt ttattaggat tattgaatat
     5581 gttactacaa gactacgctt ctgttcgaat gattatctat gctttagccc tgattttaat
     5641 tatgattttt agacctagcg gtttattagg aacgaaagag ttaactctat ctcatctatt
     5701 tcgtaagacg agtaagggag gcaattagat ggcacttctt gaagtaaaaa atttaagtaa
     5761 acatttcggt ggcctaactg ctgtaggtga tgtttcgatg aaattacata aaggtgaatt
     5821 aattggctta atcggcccca acggtgcggg taagacaacg ctttttaatc tcttaacagg
     5881 agtatacctt cctagtaaag ggacaatatc acttgatggt aagatattaa atgggaggaa
     5941 accagctaaa atagcttctt taggtttagg tagaactttt caaaatatcc gactttttaa
     6001 aaatatgact gttttagaca atgtactagt aggactcagt aatcatcact tatcacatcc
     6061 tattgctagt tttttacgat tacccaaata ctatcatagt gaaaaagcct taaggaaaaa
     6121 ggcactggag ctattagaga tttttggttt gaaagcatat caagatgctc ttgctaagaa
     6181 cctcccttat ggaaaacaac ggcgtttaga aattgttcgt gctttagcaa cagaaccaaa
     6241 aatattgttt ttggatgagc ccgcggcagg aatgaatcct caagaaactg cagaattgac
     6301 gcagttaatt agtcaaataa agagtgattt tgatattact attatgctga ttgaacatga
     6361 tatgaacttg gttatgcaag taacagagag aatttatgta ttggaatatg gtcgtctgat
     6421 tgctcacggg acacctgaag aaattaaaaa taataaacgt gttatagaag catacttggg
     6481 ggggagctat aatgaccatg ttgaaagtag aaaatctatc tattcactat ggtgttattc
     6541 aagcggtgaa tgatgtctcg tttgaagtta accaaggtga ggttgtaacc ttaattggag
     6601 ccaatggtgc aggaaagaca tcaattttga ggacaatttc tggtttagta agaccaagtc
     6661 agggttctat ttcttttatg gggaaaccta tacataagtt agcagctaga aaaattgttg
     6721 gcaatggttt agctcaggtt ccagaagggc gccacgtttt ctcaagtttg tctgttatgg
     6781 aaaatttaga aatgggagcc tttcttcaaa aggatcgtga acaaaatcaa aaaatgttaa
     6841 aaaaagtgtt tgatcgtttt cctcgcttag aagaacgtaa aaaccaagat gcagcaacct
     6901 tatcaggtgg tgagcagcaa atgctcgcta tggggcgtgc attgatgagt cgtcctaaat
     6961 tattactttt agatgaacag tcaatgggcc tagctccaat ttttattcaa gaaattttta
     7021 atatcattga agacatcaaa aaacaaggga ctacagttct tttagttgaa caaaatgcaa
     7081 ataaagctct gacgattgct gataaagcat atgttctaga gactgggaag gttgttttat
     7141 cagggacagg taaggaatta ctagtttcag atcaagttcg taaagcttac ttaggtggat
     7201 aatatttaga ggagtcaggt atgccagtga aagattttat gacaaaaaaa ttagtttatg
     7261 tctctccaga tactacagtt gcagaagctg ctgatttatt gagagaacac cacttaaggc
     7321 gtttgccagt tgttgaaaat gatcagctag tggggttagt gacagaaggg acaatggcag
     7381 aagcacaacc ttctaaagca accagtcttt ctatatatga gatgaactat cttcttaata
     7441 agacaaaaat tcgagatatt atgattaaag atattgtgac tgtttcacag tatgctagcc
     7501 ttgaggacgc tatttatttg atgatgtctc gaaagatcgg tgttttacca gtagttgata
     7561 atggacaact ttatgggatt gtcacggata gggatgtttt taaagccttc cttgaaattg
     7621 cggggtatgg tcaagaaggt tatcgcttgg ttatattagc tgatgagggg ataggggtac
     7681 tatcaaaagt cctaaatcgt ctttctagtg caaatttaag tgttaaacgt ttagtcatta
     7741 ttgaacgaaa agcaggtaag aaagcggtcg agatacaact agaaggatac gctgacaagg
     7801 atgttttaaa acaagaacta gtatttgatg atgttattgt ggaaacttta gaaaagacca
     7861 tgaaaaaacc actttcttaa aaatat
//
LOCUS       DATROQ010000083         7865 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.084, whole genome shotgun sequence.
ACCESSION   DATROQ010000083 DATROQ010000000
VERSION     DATROQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.084"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..448
                     /gene="atpB"
                     /locus_tag="VBC94_001661"
     CDS             <1..448
                     /gene="atpB"
                     /locus_tag="VBC94_001661"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEO6979919.1"
                     /translation="NLGLMTSLKTHEHNFWTSPTANFGVDITLSLLVAFICHIEGIRK
                     KGIGGYLKGFLSPTPAMLPMNLLEEVTNVASLALRLFGNIFSGEVVTGLLLQLAVLSP
                     FTGPLAFALNIVWTAFSMFIGFIQAYVFIILSSSYIGHKVHGDEEE"
     gene            466..963
                     /gene="atpF"
                     /locus_tag="VBC94_001662"
     CDS             466..963
                     /gene="atpF"
                     /locus_tag="VBC94_001662"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEO6979920.1"
                     /translation="MSILINSTTIGDIIIVSGSVLLLFILIKTFAWKQITGIFEAREQ
                     KIANDIDTAEQARQQAEAFATKREEELSNAKTEANQIIDNAKETGLAKGDQIISEAKT
                     EADRLKEKAHQDIAQNKAEALADVKGEVADLTVLLAEKIMVSNLDKEAQSNLIDSYIK
                     KLGDA"
     gene            963..1499
                     /locus_tag="VBC94_001663"
     CDS             963..1499
                     /locus_tag="VBC94_001663"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEO6979921.1"
                     /translation="MNKKTQALIEQYSKSLVEVAIEHKIVEEIQQEVAALIDIFETSE
                     LEGVLSSLAVSHDEKQHFVKTLQTSCSTYLVNFLEVIVQNEREALLYPILKSVDQELI
                     KVNGQYPIQITTAVALSPEQKERLFDIAKTKLALPNGQLVEHIDPSIVGGFVVNANNK
                     VIDASVRNQLHQFKMKLK"
     gene            1515..3020
                     /locus_tag="VBC94_001664"
     CDS             1515..3020
                     /locus_tag="VBC94_001664"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEO6979922.1"
                     /translation="MAINAQEISALIKKQIEDFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFSNGAYGMAQNLESNDVGIIILGDFSEIREGDVVKRTGKIMEVPVGEA
                     MIGRVVNPLGQPVDGLGEIETTATRPVETPAPGVMQRKSVFEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRKYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYNGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDALGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGAAQIKAMKRVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEVLKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     NDILAFEEALYDYFDAHYDNLFETIRTTKDLPEEAELDAAIQAFKDQSQFK"
     gene            3036..3917
                     /locus_tag="VBC94_001665"
     CDS             3036..3917
                     /locus_tag="VBC94_001665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEO6979923.1"
                     /translation="MAGSLSEIKDKILSTEKTSKITSAMQMVSSAKLVKSEQAARDFQ
                     VYASKIRQITTNLLKSDLVSGSDNPMLASRPVKKTGYIVITSDKGLVGGYNSKILKAM
                     MDTITDYHTENDDYAIISIGSVGSDFFKARGMNVSFELRGLEDQPSFDQVGKIIAQAV
                     EMYKNELFDELYVCYNHHVNSLTSQVRMQQMLPIKELDAEEASEDRVITGFELEPNRE
                     VILEQLLPQYTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQA
                     AITQEITEIVAGANALE"
     gene            3991..5397
                     /gene="atpD"
                     /locus_tag="VBC94_001666"
     CDS             3991..5397
                     /gene="atpD"
                     /locus_tag="VBC94_001666"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEO6979924.1"
                     /translation="MSSGKIAQVVGPVVDVVFASGDKLPEINNALIVYKNGDKSQKVV
                     LEVALELGDGLVRTIAMESTDGLTRGLEVLDTGRAISVPVGKDTLGRVFNVLGDAIDL
                     EEPFAEDAERQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALTPEIVGDEHYEVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAETFTGQPGSYVPVEETVRGFKEILDGKHDQIPEDAF
                     RMVGGIEDVIAKAEKMNY"
     gene            5410..5823
                     /locus_tag="VBC94_001667"
     CDS             5410..5823
                     /locus_tag="VBC94_001667"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEO6979925.1"
                     /translation="MAQLTVQVVTSDGIRYDHHASLITVRTPDGEMGILPGHINLIAP
                     LIVHQMKINRSHQEGVDWVAVNGGIIEVNEDQVTIVADSAERARDIDLNRAERAKERA
                     ERALEKAQTTQNIDEMRRAEVALRRAINRISVGKK"
     gene            5888..6118
                     /locus_tag="VBC94_001668"
     CDS             5888..6118
                     /locus_tag="VBC94_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEO6979926.1"
                     /translation="MILNIVTIVSNFIFIALFYQLFVDLFDWSKMIKMSPQNISKLKV
                     FILSISIVGGYVVSHFLLEVIQLCQSIFWALQ"
     gene            6181..7452
                     /gene="murA"
                     /locus_tag="VBC94_001669"
     CDS             6181..7452
                     /gene="murA"
                     /locus_tag="VBC94_001669"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO6979927.1"
                     /translation="MDKIIVEGGQTRLQGQVVIEGAKNAVLPLLAATILPSQGKTLLT
                     NVPILSDVFTMNNVVRGLDIQVDFNCDKKEILVDASGDILDVAPYEFVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPIDLHLKGLEAMGATITQNGGDITAQAEKLKGANI
                     YMDFPSVGATQNLMMAATLASGTTTIENAAREPEIVDLAQLLNKMGAKVKGAGTETLT
                     IIGVDALHGTEHDVVQDRIEAGTFMVAAAMTSGNVLVKDAIWEHNRPLISKLMEMGVE
                     VSEEEDGIRVKADTKKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMIETVFEN
                     RFQHLEEMRRMGLQTEILRDTAMIHGGRALQGAPVMSTDLRASAALILAGMVAQGQTV
                     VGQLTHLDRGYYQFHEKLAALGANIKRVSEA"
     gene            7454..7645
                     /locus_tag="VBC94_001670"
     CDS             7454..7645
                     /locus_tag="VBC94_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO6979928.1"
                     /translation="MASSGWKYVLKQIGLIVLVILLALLFLAVGLMLGYSVFGDGEHA
                     YSILSLDKWQNIIGKFLGK"
     gene            7720..>7865
                     /locus_tag="VBC94_001671"
     CDS             7720..>7865
                     /locus_tag="VBC94_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease"
                     /protein_id="HEO6979929.1"
                     /translation="MTKKQKQITASIVTLLFSLLAIGLGESKSLPNDHILKQVTDLII
                     SNESG"
BASE COUNT         2481 a         1296 c         1666 g         2422 t
ORIGIN      
        1 caacttagga ttgatgacat cattgaaaac acatgagcat aatttttgga catctccgac
       61 cgctaatttt ggtgtagata ttacattatc attattagta gcatttattt gtcatattga
      121 aggtattcgc aaaaaaggaa ttggtggtta tctcaaaggt ttcttatcac caacaccagc
      181 aatgcttccg atgaatttat tggaagaagt tactaatgtt gcttcattag cattgcgtct
      241 ttttggtaat atcttttctg gtgaagttgt cactggatta cttttacaac ttgcagtttt
      301 atcaccattt acaggtccac tagcttttgc attgaatatc gtttggacag cattttcaat
      361 gtttattggt ttcatccaag cgtatgtgtt cattatcttg agctcaagtt atattggaca
      421 caaagttcat ggtgacgaag aagaataaga aaagagagga gaaatatgtc aatacttatt
      481 aatagtacaa cgattggtga tatcattatc gtatctggct ctgtgcttct cttatttatc
      541 ttaattaaga cttttgcatg gaaacaaatc acaggtattt ttgaagcccg tgaacaaaag
      601 atagctaatg atattgatac tgctgaacag gcacgtcaac aagctgaagc ttttgcaact
      661 aagcgtgagg aagaactatc taacgctaaa actgaagcta atcaaatcat tgataatgct
      721 aaagagacag gtcttgcaaa aggtgatcaa attatttcag aagcaaaaac tgaagctgat
      781 cgattaaaag aaaaagcaca tcaagatatt gcccaaaata aggcagaagc actagcagat
      841 gttaagggtg aagtagcaga tttaacagtt ttattagctg aaaaaattat ggtatcaaat
      901 cttgataaag aagctcagag taatctcatt gatagctata ttaaaaagct aggagatgcc
      961 taatgaataa aaagacgcag gctctaattg agcaatattc aaaaagcctt gttgaagttg
     1021 ccattgaaca taaaattgtt gaagaaatcc aacaagaagt agcagcttta attgatattt
     1081 ttgaaacaag cgagcttgag ggtgtcctgt caagtcttgc cgtttcacat gatgaaaaac
     1141 aacattttgt gaagacattg caaacatcat gttcaactta tcttgttaat ttccttgagg
     1201 ttattgtaca aaatgaacgt gaagcacttc tatatcctat tttgaaatca gttgatcagg
     1261 aacttatcaa agtgaatggt caatatccaa ttcaaattac aactgcagtt gctttgtctc
     1321 cagaacaaaa agaacgtctt tttgatattg ctaaaactaa attagcttta ccaaatggac
     1381 agttagttga gcacattgac ccatctattg ttggtggatt tgttgtcaat gcaaacaata
     1441 aagtaattga tgcaagtgtt cgtaatcaat tacatcaatt taaaatgaaa ttaaaataga
     1501 aagtggtgtg acttttggca attaatgcac aagaaattag cgctttaatt aaaaagcaaa
     1561 ttgaagactt ccagccaaat tttgacgtca cagaaactgg tattgttaca tatatcggtg
     1621 atggtattgc tcgtgctcgt gggctagata atgctatgag tggagaactt cttgaatttt
     1681 caaatggtgc gtacggtatg gctcaaaacc ttgagtctaa tgacgtgggt atcattattt
     1741 tgggtgattt ctcagaaatt cgtgaaggtg atgtggtaaa acgtactggt aaaattatgg
     1801 aagtaccagt aggggaagct atgattggac gtgtcgtcaa tccgcttggt caaccagttg
     1861 atggactagg tgaaattgag acgacagcaa cacgcccagt tgaaacacca gcacctggtg
     1921 ttatgcaacg taaatcagtt tttgagcctc ttcaaacagg tctcaaagcg attgatgctt
     1981 tggttcctat cggacgtggg caacgcgaat tgattatcgg tgaccgccaa actggtaaga
     2041 cgtctgtagc gattgatgca atcttgaatc aaaaaggtca agatatgatc tgtatctatg
     2101 tggctatcgg tcaaaaagag tctacagtac gtacacaagt tgaaactttg cgtaaatatg
     2161 gtgcccttga ttacacaatc gttgtgacag cttcagcatc acaaccatct ccattacttt
     2221 tcattgctcc atatgcagga gtagcaatgg ctgaggaatt catgtataac gggaaacacg
     2281 ttttgattgt ttatgatgat ttatcaaaac aagccgtagc ttatcgtgaa ctttcgcttc
     2341 ttcttcgccg tcctccaggt cgcgaagctt acccagggga tgttttctat cttcatagtc
     2401 gtcttttgga acgttcagct aaagtatcag atgctttagg tggaggttcg attacagcgc
     2461 ttccgttcat tgaaacacaa gcaggtgata tctcagcata tattgctaca aacgttattt
     2521 caattactga cggccaaatt ttcttgcaag aaaatctctt taactcaggt attcgtccgg
     2581 cgattgatgc aggttcatca gtatctcgtg ttggtggtgc agcgcaaatt aaagcgatga
     2641 agagagttgc aggtacgctt cgtctagatc ttgcttctta tcgcgaactt gaggcattca
     2701 ctcaatttgg ttcagacctt gatgcagcaa cacaagcaaa actaaatcgt ggtcgtcgca
     2761 cagtggaagt gttaaaacaa ccattacaca aaccattgcc agttgagaaa caagttgtga
     2821 ttttatatgc tctaacacat ggattccttg atgatgtacc tgtaaatgat attttagctt
     2881 ttgaagaagc tttgtatgat tactttgatg ctcattatga taatctcttc gaaacaatcc
     2941 gcacaacaaa agatttacca gaagaagcag aattagatgc tgctattcaa gcatttaaag
     3001 atcaatcaca atttaaataa taggagggtt ctaatatggc aggctctctt agtgaaatta
     3061 aagacaagat tctttctaca gaaaaaacaa gtaagattac tagtgcgatg cagatggtct
     3121 catctgccaa gttggttaaa tctgaacaag ctgctagaga ttttcaagtg tatgcctcta
     3181 aaattcgtca aattactact aacctcttga agtcagactt agtatctgga tcagataacc
     3241 ctatgttagc ttcacgtccg gtgaagaaga caggttatat tgttattact tctgataagg
     3301 gtttagttgg tggttacaat tcaaaaatct taaaagcgat gatggacact ataactgact
     3361 atcatactga gaatgatgat tatgccatta tttcaattgg tagtgttggt tctgattttt
     3421 tcaaagcacg tggaatgaat gtttctttcg aattacgtgg tttagaggac caaccaagtt
     3481 ttgatcaagt tggtaaaatt attgctcaag cagttgaaat gtacaaaaat gagttgttcg
     3541 atgaattata tgtttgctac aatcaccatg ttaacagttt aactagccaa gtcagaatgc
     3601 aacaaatgct tcctatcaaa gaattggatg cagaagaggc ttctgaggat agagtgatta
     3661 ctggttttga acttgagcca aaccgtgaag ttatattgga gcaattattg cctcaatata
     3721 ctgagagttt gatttatgga gctattattg atgctaaaac agcagagcat gcggctggta
     3781 tgacagctat gcagactgca accgataatg ctaaaaatgt tatcaatgat ttaactattc
     3841 aatataaccg tgctcgtcaa gcagctatca cacaggaaat tacagaaatt gttgcaggtg
     3901 ctaatgcact tgaataatta attagtctat agaaaaaata gtgaacaaag gtattttaaa
     3961 tcctttgtat catatgataa ggagaaatac atgagctcag gcaaaattgc tcaggtagtt
     4021 ggacctgtcg ttgacgttgt atttgcaagt ggcgacaaac ttcctgagat taataatgca
     4081 ttgattgtct ataaaaatgg cgataagtca caaaaagtag tacttgaagt tgctcttgaa
     4141 cttggtgacg gcctcgttcg tacaatagct atggaatcaa ctgatgggct tacacgtggt
     4201 ttggaagtat tagatactgg tcgcgcaatt agtgtgccgg ttggtaagga tactttgggt
     4261 cgtgtcttca acgttcttgg agatgctatt gaccttgaag agccttttgc agaagatgca
     4321 gaacgtcaac caatccataa aaaagcacca tcgtttgatg aattatcaac atcatcagaa
     4381 atcttagaaa caggtattaa agttattgac ttattagcac cttacttaaa aggtggtaaa
     4441 gtcgggcttt tcggtggtgc gggtgttggt aaaaccgttc ttattcaaga attaatccac
     4501 aacatcgccc aagaacatgg tggtatttca gtatttactg gtgtaggaga acgtactcgt
     4561 gaagggaatg acctttattg ggaaatgaaa gaatctggcg ttattgaaaa aacggctatg
     4621 gtctttggtc aaatgaatga accaccagga gcacgtatgc gtgtggcact tactggtctt
     4681 acaatagctg aatacttccg tgatgtagaa ggacaagatg tgcttctctt cattgataat
     4741 atcttccgtt tcacacaagc ggggtcagaa gtgtcagcgc ttttaggtcg tatgccttca
     4801 gccgttggtt atcaaccaac acttgctaca gaaatgggac aattacaaga gcgtatcact
     4861 tcaactaaaa aaggttctgt tacatcaatc caagctattt atgtgccagc agatgactat
     4921 actgaccctg cgccagctac agcctttgct cacttagatt caacaactaa ccttgaacgt
     4981 aaattgacac aaatggggat ttatccagcc gttgacccat tagcttcaag ttctcgtgca
     5041 ctcacaccag aaatcgttgg agatgagcac tatgaagttg caacagaagt acagcgtgtt
     5101 cttcaacgtt accgtgagtt acaagatatc attgcgattt taggtatgga tgaattatca
     5161 gatgaggaaa aaacgttagt tggacgtgca cgtcgtatcc aattcttcct ttctcaaaac
     5221 ttcaatgttg cagaaacatt tactggtcaa ccaggttcat atgttccggt tgaagaaact
     5281 gttcgcggat tcaaagaaat cctagatggt aaacatgatc aaattccaga agatgccttc
     5341 cgtatggttg gtggtattga ggatgttatc gcaaaagcag aaaaaatgaa ctattaatga
     5401 ggtgatgcta tggcacaatt gactgttcaa gtagtaacat cagatggtat tagatatgac
     5461 catcacgcta gtctcattac tgttagaacg ccagatggtg aaatgggaat tttaccaggt
     5521 cacataaatt tgattgctcc ccttattgtt catcagatga agattaatcg ttctcatcaa
     5581 gaaggtgtag actgggtagc tgttaatggt ggtataattg aagttaatga ggatcaggtg
     5641 acaattgttg cggattcagc agaacgagca cgtgatatag accttaaccg tgcagaacgg
     5701 gctaaggaac gcgctgagcg tgcattagaa aaagctcaaa caactcagaa tattgatgag
     5761 atgcgtagag ctgaggttgc tcttcgtcgc gcaattaacc gtattagcgt cggtaaaaag
     5821 tagaaaaatg gtctaattag ttttgtgact aattaggctt ttttagtgta taatagaagg
     5881 catgaatatg atattaaata tagtaactat agttagtaat tttattttta ttgccctttt
     5941 ttatcaactt tttgtagatt tatttgactg gtctaaaatg attaaaatgt cccctcaaaa
     6001 catctcaaaa ttaaaagttt ttattttgtc gataagtata gttggtggct atgtggttag
     6061 tcatttttta ttggaagtga ttcagttgtg tcaatcaatc ttttgggctc tccaataaag
     6121 gaaatattag ctgaactatg atataatcaa cttactattg ttataagaat ggagattcaa
     6181 atggataaaa ttattgtgga aggtgggcaa acgcggcttc aaggtcaagt tgttattgaa
     6241 ggagctaaaa atgcagtttt acctctttta gcagccacaa ttttaccaag ccaagggaaa
     6301 acattattga ccaatgttcc tatcttgtct gatgtattta caatgaacaa tgttgttcgt
     6361 ggtttagata ttcaagttga ctttaattgc gataaaaaag aaatcttagt ggatgcctca
     6421 ggtgatattt tagatgttgc accttatgaa tttgttagtc aaatgagagc ttctattgtt
     6481 gttttgggac ctattttagc tagaaatggc catgctaagg tttcaatgcc aggcggttgt
     6541 acaattggta gccgaccaat tgaccttcat cttaaaggtt tagaagctat gggagctacg
     6601 attacacaaa atggtggtga tattactgcc caagctgaaa agcttaaggg ggctaatata
     6661 tacatggatt ttccctcagt cggtgccact caaaacttga tgatggctgc tacacttgct
     6721 tcaggcacaa caacgataga gaatgctgca cgtgaaccag aaattgtaga ccttgcgcag
     6781 ttactaaata aaatgggagc taaagtcaag ggtgctggta cggaaacatt aacgatcatt
     6841 ggtgttgatg ctttacatgg tacagaacat gatgttgtcc aagatcgtat tgaagctgga
     6901 acctttatgg tggcagctgc tatgacttct ggaaatgttc tagtaaagga tgctatttgg
     6961 gaacataacc gtcctttaat ctctaaattg atggagatgg gtgttgaagt gtcagaggaa
     7021 gaagatggta ttcgtgtaaa agctgatact aaaaaattga agccagtgac tgtgaaaaca
     7081 ctccctcatc caggatttcc tactgatatg caagctcaat ttacagctct aatggcagta
     7141 gttaatggcg aatcaactat gattgagaca gtttttgaaa atcgttttca acatttggaa
     7201 gaaatgcgtc gcatgggatt acaaacggaa attttacgag atactgcaat gattcatggt
     7261 gggagagcct tacaaggtgc accggtcatg tctactgact tacgagctag tgcagcttta
     7321 atcttggcag gaatggttgc gcaagggcaa acagtagttg gccaattaac ccatttagac
     7381 cgtggttatt atcaattcca tgaaaaatta gcggctcttg gtgctaatat taaacgtgta
     7441 agtgaggcgt aatatggcat ctagtggttg gaaatatgta ctaaaacaaa tcggtctaat
     7501 agtattagtc atactgttag ccttgctgtt tttagcggtt ggattgatgt tgggttatag
     7561 tgtttttggt gacggagagc atgcttactc tattttatcg ctggataaat ggcaaaatat
     7621 tattggaaaa tttttaggaa aatagaaaga gccactataa gttatttgat gcttatagtg
     7681 gtttcctttg agagttaatt ttagcagtag aataatacaa tgacaaaaaa acaaaaacaa
     7741 ataacagctt cgattgtaac tttgcttttt tcattactgg caatagggtt aggtgaaagt
     7801 aaatctttgc ctaatgatca cattttaaaa caggtgacag atttaattat tagtaatgaa
     7861 tctgg
//
LOCUS       DATROQ010000084         7812 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.085, whole genome shotgun sequence.
ACCESSION   DATROQ010000084 DATROQ010000000
VERSION     DATROQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.085"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            96..2348
                     /gene="gshAB"
                     /locus_tag="VBC94_001672"
     CDS             96..2348
                     /gene="gshAB"
                     /locus_tag="VBC94_001672"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEO6979930.1"
                     /translation="MIIDQLLQRSHSHLPILQATFGLERESLRIHQPTQRVAQTPHPK
                     TLGSRNYHPYIQTDYSEPQLELITPIAKDSQEAIRFLKAISDVAGRSINHDEYLWPLS
                     MPPKVREEDIQIAQLEDAFEYDYRKYLEKTYGKLIQSISGIHYNLGLGQELLTSLFEL
                     SQADNAIDFQNQLYMKLSQNFLRYRWLLTYLYGASPVAEEDFLNQKLNNPVRSLRNSH
                     LGYVNHKDIRISYTSLKDYVNDLENAVKSGQLIAEKEFYSPVRLRGSKSCRNYLEKGI
                     TYLEFRTFDLNPFSPIGITQETVDTVHLFLLALLWIDSSSHIDQDIKEANRLNDLIAL
                     SHPLEKLPNQAPVSDLVDAMQSVIQHFNLPPYYQDLLESVKRQIQSPKLTVAGQLLEM
                     IEGLSLETFGQRQGQIYHDYAWEAPYALKGYETMELSTQLLLFDVIQKGVNFEVLDEQ
                     DQFLKLWHNSHIEYVKNGNMTSKDNYIVPLAMANKVVTKKILDEKHFPTPFGDEFTDR
                     KEALNYFSQIQDKPIVVKPKSTNFGLGISIFKTSANLASYEKAIDIAFAEDSAILVEE
                     YIEGTEYRFFVLEGDCIAVLLRVAANVVGDGIHTISQLVKLKNQNPLRGYDHRSPLEV
                     IELGEVEQLMLEQQGYTVNSIPPEGTKIELRRNSNISTGGDSIDVTNTMDPTYKQLAA
                     EMAEAMGAWVCGVDLIIPNATQAYSKDKKNATCIELNFNPLMYMHTYCQEGPGQSITP
                     RILAKLFPEL"
     gene            2472..3290
                     /locus_tag="VBC94_001673"
     CDS             2472..3290
                     /locus_tag="VBC94_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979931.1"
                     /translation="MVKKRYSKNSHNLITLLGIVVLASLISDFWSEVIATLLIIGGGY
                     CAYYVYDKKRLKCFTSNQRIEALKSDIKETDQDIRHLEILKKDNRSKEYIKLAHQILP
                     QLDLIRNEANQLQKAIEPNIYKRITKKANTFSNEINEQLIKLHASPELEPISDQEDEM
                     IRIAPELKPFYHNIQDDHYAILKKIEEADNKAELAAIHQANMKRFTDVLAGYIRIKQS
                     PKNFNNAKERLEQALQAIKKFNLDLDETLRQLNESDMKDFDVSLRMMQDERNNK"
     gene            3287..4543
                     /locus_tag="VBC94_001674"
     CDS             3287..4543
                     /locus_tag="VBC94_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEO6979932.1"
                     /translation="MSDTFNFDIDQIADNAITKTDKTTEIISNQTTSQTGQIAFFEKL
                     TPAQKSAISEKTPALVDTFVGDQNALLDFGQSAVEGVNTTVNHILSEQKKIQIPQVDD
                     LLKNANRELNGFIAKYKDATPAELEKKPNLIQKLFKQSKTSLQEFYFDSQNIEQKMDM
                     MAANVVKQEDTLARNIVSAEMLIEDNTKSIENLVGVIAFIESSQAEAANRASHLQQEI
                     LALDSQTSEYQIKSNQLARMTEVINTLEQQHTEYVSRLYVAWATTPQMRNLVKVSSDM
                     RQKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGVTADAIVNANNAALQMLAETSKEAIP
                     MLEKTAQSPTVSIKSVTALAESLVAQNNGIIAAIDKGRKERAQLESAVIKSAETINDS
                     VKIRDKKIVEALLNEGKSTQEKVDES"
     gene            4683..5558
                     /gene="hslO"
                     /locus_tag="VBC94_001675"
     CDS             4683..5558
                     /gene="hslO"
                     /locus_tag="VBC94_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEO6979933.1"
                     /translation="MDKIIKSISTSGSFRAYVLDCTETVRTAQEKHQTLSSSTVALGR
                     TLIANQILAANQKGNSKVTVKVIGDSSFGHIISVADTKGNVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVVITDYGTGQPYTSTTPLITGEIGEDFAYYLTESEQTPSAVGLNV
                     LLDDEDKVKVAGGFMLQVLPGASDEEISRYEKRIQEMPSISSLLESENHIESLLSAIY
                     GEDDYKRLSEDSLAFYCDCSKERFEAALLTLGTKELQAMKDEDKGVEITCQFCNQTYY
                     FTEEDLEKIINDSIK"
     gene            5542..6519
                     /gene="dusB"
                     /locus_tag="VBC94_001676"
     CDS             5542..6519
                     /gene="dusB"
                     /locus_tag="VBC94_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEO6979934.1"
                     /translation="MTQLNSSFMIGKVEIPHRTVLAPMAGITNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQNNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPEKIYHIVKEVTSVLDIPLTVKMRTGWSDSSNAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLGKVAKAVTSIPFIANGDIRTVHDAK
                     FMIEEIGADAIMVGRGARSNPYIFTQINHFFETGEILPDLPFEKMLDVAEDHLTRLVN
                     LKGETIAVREFRGLAPHYLRGKSGAAKIRGAVSRAETLAEVQELFAGLR"
     gene            6640..7281
                     /locus_tag="VBC94_001677"
     CDS             6640..7281
                     /locus_tag="VBC94_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEO6979935.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGQHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIFFLNKRFQSIKEAYKANNNVLDRSIFEDELFLTLNFKNGNVTKTELD
                     IYKELLANMLEELEGMPKKRPDLLVYIDVSFDKMLERIDKRGRSFEQVDSNPELYDYY
                     KQVHSEYPEWYENYDVSPKIRIDGNKLDFVKNPEDLQHVLDTIDSELQKLDLL"
     gene            7295..7786
                     /locus_tag="VBC94_001678"
     CDS             7295..7786
                     /locus_tag="VBC94_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEO6979936.1"
                     /translation="MIIRPVLPSDAKELLAIYAPYVTDTTITFEYDIPSISEFTNRIK
                     NISKSFPYLVAEENGKILGYAYASTYYARASYDWTCELSIYLDKDVRSKKIGSQLYDT
                     LEKELVNQGYVNLLACISLPNDISIAFHKKRGFNQVAHFPKIGFKFEQWHDIVWLQKR
                     LKS"
BASE COUNT         2680 a         1419 c         1390 g         2323 t
ORIGIN      
        1 agtataagat tattttagtt ttctgtgccg taaaacttag tttttattat ttttttgata
       61 aactagtatc atctgttact aggaggaatg ttgtcgtgat tatcgatcaa ctgttacaaa
      121 gaagccactc tcatctaccg attctacaag ctacatttgg cttagagaga gaaagccttc
      181 gtattcacca acccactcaa agggttgctc aaacacccca tcccaaaaca ttagggagtc
      241 gtaactatca tccttatatc caaacggatt atagtgaacc tcaattagaa ctcattacac
      301 ctattgcaaa ggatagccaa gaagccattc gatttttgaa agctataagt gatgtcgctg
      361 gacgatctat caaccacgat gaatacttat ggccgctttc tatgccaccc aaagtgaggg
      421 aagaagacat acagattgcc caattagagg atgcttttga atatgattat cgcaaatatt
      481 tggagaaaac ttatggaaaa ctaatacaat ccatctctgg aattcactat aatctcggtt
      541 taggtcaaga gttattaact tctctctttg aattgagtca agcagataat gctattgatt
      601 ttcagaacca gctttacatg aaattgtctc aaaatttttt acgttaccgt tggttactaa
      661 cttatctata cggagcgagc ccagtagctg aagaagactt tttaaatcag aaacttaata
      721 accctgtccg gtcacttaga aatagtcact taggctatgt gaatcataaa gatattcgta
      781 tttcttatac tagcttgaag gattatgtca acgatctaga aaatgctgta aaaagtgggc
      841 aattgattgc tgagaaagaa ttttattcac ctgttcgtct ccgtggtagt aagtcctgtc
      901 gtaattattt agaaaaagga attacatatt tagaatttcg cacttttgat ctcaacccat
      961 ttagcccaat cggtataacg caggaaactg ttgatactgt tcaccttttc ttattggcgc
     1021 ttttgtggat agattctagt agtcatattg accaagatat caaggaagcc aatagattaa
     1081 atgaccttat agcactcagt catccattag aaaaattacc taatcaagca ccagtttctg
     1141 acttagtgga tgctatgcaa tctgttatcc aacattttaa cttaccacct tactatcaag
     1201 acctattgga atctgtaaaa aggcaaattc agtcacctaa attaacggta gctggtcaac
     1261 ttttagaaat gattgaagga ctttccttag aaacatttgg acaaagacaa ggacaaattt
     1321 atcacgatta tgcttgggaa gctccctacg ccttaaaggg atatgaaacg atggaacttt
     1381 ctacccaatt gctactattt gacgttatcc aaaaaggagt caacttcgaa gtactggatg
     1441 aacaagatca attcctaaaa ttatggcaca atagtcatat tgagtacgtt aaaaatggta
     1501 atatgacctc aaaagataac tatattgtac cgctcgctat ggctaacaaa gttgttacaa
     1561 aaaaaatcct agatgaaaag catttcccaa ctccttttgg agatgaattt actgaccgta
     1621 aagaggcact taactatttt tcacaaatcc aagataaacc aatcgtcgtt aagccaaaat
     1681 ctacaaactt tggtttaggc atttctattt ttaaaacatc agctaactta gcttcttatg
     1741 aaaaagcaat tgacattgct tttgccgaag atagcgctat tcttgtggaa gaatatattg
     1801 agggtaccga ataccgtttc ttcgtccttg agggggactg tattgctgta ttgcttaggg
     1861 tagcagctaa tgtcgttgga gatggtattc atactattag tcagctagtt aaacttaaaa
     1921 accaaaaccc tctcagaggc tatgatcatc gctctcctct agaggttatt gagctcggag
     1981 aagtagaaca gttaatgttg gaacaacaag gatacactgt taacagtatc cctccagaag
     2041 gaacaaaaat agaacttcgc cgtaattcta atatttccac tggcggtgac tctatagatg
     2101 taacaaatac aatggatcct acatataaac aactagcagc tgagatggca gaggctatgg
     2161 gggcttgggt ttgtggagtt gatttaatta ttcctaatgc cactcaagca tactcaaaag
     2221 ataagaaaaa tgctacttgc attgaactaa actttaaccc attgatgtat atgcacacct
     2281 actgtcaaga ggggcctggt caatctatta cacctcgaat tttggctaaa ctgttcccag
     2341 aattataact aaaaagagtt ccttaggcta agcttaagga atttttggtt aaaataaagt
     2401 tatcccttgt catcactagt tcatttaaga taaaatatta acaactacag aaagttaggt
     2461 taaatattat tatggttaaa aaacgctatt ccaaaaattc tcataatcta ataacattac
     2521 ttggtattgt tgttcttgct tccctgattt cagacttttg gagtgaggtg attgctactc
     2581 tgttaattat tggtggtggt tattgtgcct attacgttta tgataaaaag cgtcttaaat
     2641 gttttacatc aaaccaacgt attgaagctt taaaatccga tattaaagag actgaccaag
     2701 acattagaca cttggaaatt ttaaaaaaag acaatcgttc taaggaatat atcaaattag
     2761 cacatcaaat attacctcaa ttggacctaa ttcgtaatga agcaaaccaa ctacaaaaag
     2821 ccattgagcc taatatctat aaacgtatca ccaaaaaagc aaatacattt agtaatgaga
     2881 ttaacgaaca attaataaag ctacatgcct ctccagaact agaacctatt tctgatcagg
     2941 aagatgaaat gatacgcatt gctccggaat taaaaccgtt ctatcacaat attcaagatg
     3001 atcactatgc tatcttaaaa aagattgaag aggcagataa caaagctgaa ttagcagcaa
     3061 ttcatcaagc caatatgaaa cgttttacag atgttctggc tggctacatc agaatcaaac
     3121 aatctcctaa aaattttaac aatgctaagg aacgcctaga acaagcatta caagcaatta
     3181 aaaaatttaa tttagattta gatgaaacgc tgcgccaatt gaatgaaagt gatatgaaag
     3241 attttgacgt tagcctaaga atgatgcaag acgaaaggaa caataaatga gcgatacctt
     3301 taattttgat attgaccaaa ttgcagacaa tgctatcact aaaacagata aaacaacaga
     3361 aattatttcc aaccagacaa caagccaaac tgggcaaatt gccttttttg aaaaactaac
     3421 accagcacaa aagtctgcta tctctgaaaa aacaccagct ttggtagata cttttgtcgg
     3481 tgaccaaaat gcgctccttg attttggaca atccgcagta gaaggcgtta atactactgt
     3541 taatcatatc ttgtctgagc agaaaaaaat tcaaattcct caagttgatg atttactaaa
     3601 aaatgctaat cgcgaactaa atggatttat tgccaaatat aaagatgcta ctccggcaga
     3661 attagagaaa aaaccaaact tgattcaaaa attattcaaa caaagcaaga cctcgctaca
     3721 ggaattttat tttgactcac aaaacatcga gcaaaaaatg gatatgatgg cagcaaatgt
     3781 tgtcaaacaa gaagatactt tggcaagaaa tatcgtctct gctgaaatgc tcattgaaga
     3841 taatactaaa tctattgaaa atttggttgg agttattgct tttattgaat cgagtcaagc
     3901 cgaggctgcc aatcgtgcaa gccacttaca acaagaaatt ctagcattag atagccaaac
     3961 gtccgaatat caaattaaaa gtaaccaatt agcccgaatg actgaagtta tcaataccct
     4021 cgaacagcaa catacggaat atgtcagccg tctctacgtt gcatgggcaa caacaccaca
     4081 gatgcgaaac ttggtcaaag tatcgtcaga tatgcgtcag aaacttggta tgttacgtcg
     4141 aaataccatt ccaacaatga aactctcaat cgctcagtta ggcatgatgc aacaatctgt
     4201 caaatccggt gtcactgctg atgctattgt caacgctaat aatgcagcat tgcaaatgct
     4261 ggctgaaact agtaaagaag cgattccgat gttagagaag accgcacaaa gccccactgt
     4321 ttctattaaa tctgtcactg cattagctga aagcttagtg gctcaaaata atggtattat
     4381 cgctgccata gacaaaggac gtaaggaacg tgcccaatta gaatctgctg ttattaaatc
     4441 ggctgaaaca atcaatgatt ctgtcaaaat tcgtgataaa aaaatagttg aagccttact
     4501 caacgaaggt aaatctaccc aagaaaaagt tgatgagtct taacttacta tcatatccac
     4561 tatataaaaa cgatactgta aaacacagta tcgtttttat atcattaatt tataccgttt
     4621 tcatctatat tttcctgact atctccaatg caaaataagc cttttatgct ataatatgac
     4681 ttatggataa aattataaaa tcaatttcaa cttccggttc tttccgggcc tatgtccttg
     4741 attgtacaga aactgtaaga acagctcaag aaaaacatca gacactttca agttctactg
     4801 tagctcttgg gagaacatta attgcaaatc aaattttagc agcaaatcaa aaaggaaata
     4861 gtaaagtaac tgtcaaggtt attggagata gctcttttgg tcatattatt tctgtagctg
     4921 atactaaagg aaatgttaaa ggttacatcc aaaatacggg tgttgatatc aagaaaacag
     4981 ctactggaga ggtcttagtt ggacctttca tggggaatgg acactttgtc gttattaccg
     5041 attacggaac tgggcaacct tacacttcaa caactccact aattacagga gaaattggag
     5101 aagattttgc ttattattta accgaaagtg agcaaacacc ttctgccgtc ggtttaaatg
     5161 tcctcttaga tgacgaggat aaggttaaag ttgcaggtgg attcatgctt caggtgctac
     5221 caggtgcttc tgacgaagaa atttctcgct atgaaaaacg cattcaggag atgccttcta
     5281 tttcaagtct gctagagtct gaaaatcata tagaatctct cctttcagct atttacggag
     5341 aagacgacta caagcgctta tcggaagact ctttagcctt ctattgtgac tgttcaaaag
     5401 aacgttttga agccgctcta ttgactttag gaactaaaga attgcaagcc atgaaggatg
     5461 aagataaagg tgttgaaatt acttgtcaat tttgcaacca gacttactat ttcacagaag
     5521 aagatttgga gaagataatt aatgactcaa ttaaatagtt catttatgat tgggaaggtt
     5581 gaaattcctc atcgtaccgt actagcaccg atggcaggta ttactaattc tgcttttcgt
     5641 actatcgcta aagaatttgg tgctggtctc gttgttatgg aaatgatttc tgagaaaggg
     5701 ttactttata acaacgaaaa gaccttacat atgctccata tcgatgaaaa tgaacatcct
     5761 atgtcaattc aactatttgg tggcgatgca gaaggcttaa aacgtgcagc tgactttatc
     5821 caaaataata cgaaagcaga tatcgtagac atcaatatgg gatgtccagt taacaaagtt
     5881 gttaaaaacg aagctggagc taaatggtta cgtgaccctg aaaaaattta tcatattgtt
     5941 aaagaggtaa cgtctgtact tgacattcca ttaacagtaa aaatgcgtac aggatggtct
     6001 gactcatcta atgctgtaga aaatgcactc gctgccgaat ctgctggtgt atctgcactt
     6061 gctatgcacg gtcgaacacg tgaacaaatg tatactggca cgtgtgacca tgaaacactt
     6121 ggcaaggtcg ctaaagctgt tactagtatt ccttttattg ccaatggtga cattcgtact
     6181 gttcacgatg ccaaatttat gattgaagag attggagctg acgctataat ggttggacgt
     6241 ggtgctcgta gcaatcctta catctttaca caaattaatc atttctttga aacaggcgaa
     6301 atccttcccg acctaccatt tgagaagatg ttagatgttg ccgaggatca tctcactcga
     6361 ctagttaatt taaaaggtga aactattgcc gttcgtgaat ttcgagggtt agcacctcat
     6421 tatctacgtg gaaaatcagg cgcagcaaaa attagaggag ctgtttcacg tgcagaaaca
     6481 ctagcagaag ttcaagaact ttttgcaggt ttacgctaaa tcaaaaacag tcctttgact
     6541 gttttttcct atctctgtac aaatttagca taaatcattg tgtaatgaaa actcaaaagt
     6601 ataacaaatt attttagtat aacagaatta aggagaatta tgttgattgt tttagctgga
     6661 accattggag caggtaaaag ctcccttgca gctgctctag gacaacacct agggacagat
     6721 gttttctatg aggcggttga taataaccca gttttagatc tttactatca agatcccaaa
     6781 aaatatgctt ttttactaca aatattcttt ttaaataaac gattccaatc tataaaagaa
     6841 gcctataagg ctaacaacaa tgttttggat cgttctattt ttgaagatga actattcctc
     6901 actctaaatt ttaagaatgg aaatgtgact aaaacagagc tagatatcta taaagaactt
     6961 cttgcaaata tgttggaaga attagaaggt atgcctaaga agagacctga tttgctagtc
     7021 tacattgatg tttcatttga taaaatgtta gaacgtattg acaaacgagg acgcagcttt
     7081 gagcaagtag attctaaccc agaattatac gactactaca aacaagtcca ctctgaatat
     7141 ccagaatggt atgaaaatta cgacgtttca cctaaaattc ggatcgatgg taacaaactt
     7201 gatttcgtca aaaatccaga ggatcttcaa cacgtccttg acactattga ttccgaattg
     7261 caaaaacttg atttactata attaggtgat atctatgatt ataagacctg tcctaccctc
     7321 agatgctaaa gaattacttg ctatctatgc tccctacgtt actgacacta caataacgtt
     7381 tgaatacgat attcctagta tatcggaatt cactaatcgt atcaaaaata tcagcaaatc
     7441 tttcccatat ttagttgctg aagaaaatgg gaaaatatta ggttatgcat atgcttcaac
     7501 ttactacgct agagcttcct atgactggac ttgcgagctt tctatttacc ttgataaaga
     7561 tgtacgtagc aaaaaaattg gtagccaact atatgataca ttggagaaag aattagttaa
     7621 ccaagggtat gttaatctac tcgcttgtat cagtttacca aatgatataa gcattgcatt
     7681 ccacaaaaaa agaggattta atcaagtcgc acatttccca aaaatcgggt ttaaatttga
     7741 acaatggcat gacattgtat ggttacaaaa gcgattgaaa tcttaaggta atctttagat
     7801 ttattttaag tt
//
LOCUS       DATROQ010000085         7669 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.086, whole genome shotgun sequence.
ACCESSION   DATROQ010000085 DATROQ010000000
VERSION     DATROQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.086"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            432..1442
                     /gene="nrdF"
                     /locus_tag="VBC94_001679"
     CDS             432..1442
                     /gene="nrdF"
                     /locus_tag="VBC94_001679"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO6979937.1"
                     /translation="MQSYYDRSQSPLDYALSEKAFPMRSVNWNKLNDDKDLEVWNRVT
                     QNFWLPEKIPVSNDLNSWRTLDADWQQLITRTFTGLTLLDSVQATVGDIAQIKHSQTD
                     HEQVIYANFAFMVAIHARSYGTIFSTLCTSQQIEEAHEWVVDTESLQARSRILIPFYT
                     GDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGY
                     KYQQKVAKLSVEKQAEMKTFVFDLLYQLIDLEKAYLYELYDGFDLAEDAIRFSIYNAG
                     KFLQNLGYDSPFTEEETRISPEVFAQLSARADENHDFFSGNGSSYIMGITEETLDEDW
                     EF"
     gene            1443..1856
                     /gene="nrdI"
                     /locus_tag="VBC94_001680"
     CDS             1443..1856
                     /gene="nrdI"
                     /locus_tag="VBC94_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO6979938.1"
                     /translation="MDSTVKVVYFSSKSNNTHRFVQKLACSNQRIPSDGSSILVTEDY
                     ILIVPTYAGGGDDTKGAVPKQVVQFLNVRQNREHCQGVISSGNTNFGDTYAIAGPIIA
                     RKLNVPLLHQFELLGTQEDVTRVKELLCQFTRKDK"
     gene            1858..4023
                     /gene="nrdE"
                     /locus_tag="VBC94_001681"
     CDS             1858..4023
                     /gene="nrdE"
                     /locus_tag="VBC94_001681"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO6979939.1"
                     /translation="MTQSDAYLSLNAKTRFRDRTGNYHFTSDKEAVEQYMIEHVEPNT
                     MVFTSLIEKLDYLVSNNYYESDLLKQYNLEFICQIFEHAYAKKFAFLNFMGALKFYNA
                     YALKTEDNRYYLEHYEDRVVMNALFLAAGDEKAAYDLVDDMLANRFQPATPTFLNAGK
                     KRRGEYISCYLLRIEDNMESISRAISTSLQLSKRGGGVALCLTNLREFGAPIKGIKNQ
                     ATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKS
                     LSLGLVIPDITFELAKANKDMALFSPYDIERVYGKPMSDISITEEYETLLANADIRKT
                     FISARKLFQTIAELHFESGYPYILFEDTVNAKNPHKKEGRIVMSNLCSEIAQVNTASQ
                     FSEDLTFTKVGHDVCCNLGSINIARAMDQAADFEKLIANSIRALDRVSRTSDLDSAPS
                     IKKGNAANHAVGLGAMNLHGFLATNHIYYDSQEAIDFTDCFFYAMAYYAFKASNHLAK
                     EKGTFEGFSESSYADGSYFYQYTEQNFEPKTQRVKNLLAEYGLTLPSQEDWRKLVQSI
                     KEIGLANAHLLAVAPTGSISYLSSCTPSLQPVVSPVEVRKEGALGRVYVPAYKIDADN
                     YVYYKKGAYEVGSEAIINIAAAAQKHIDQAISLTLFMTDQATTRDLNKAYIQAFKQKC
                     ASIYYVRVRQDILEGSESYDDMLDDFTSSDLEDCQSCMI"
     gene            4086..7253
                     /locus_tag="VBC94_001682"
     CDS             4086..7253
                     /locus_tag="VBC94_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEO6979940.1"
                     /translation="MTKKHLKTLALALTTVSVVTYSQEVYGLEREESVKQEQTQSASE
                     DDWFEEDNERKTNVSKENSTVDETVSDLFSDGNSNNSSSKTESVVSDPKQVPKAKPEV
                     TQEASNSSNDASKVEVPKQDTASKKETLETSTWEAKDFVTRGDTLVGFSKSGINKLSQ
                     TSHLVLPSHAADGTQLTQVASFAFTPDKKTAIAEYTSRLGENGKPSRLDIDQKEIIDE
                     GEIFNAYQLTKLTIPNGYKSIGQDAFVDNKNIAEVNLPESLETISDYAFAHMSLKQVK
                     LPDNLKVIGELAFFDNQIGGKLYLPRHLIKLAERAFKSNRIQTVEFLGSKLKVIGEAS
                     FQDNNLRNVMLPDGLEKIESEAFTGNPGDEHYNNQVVLRTRTGQNPHQLATENTYVNP
                     DKSLWRATPDMDYTKWLEEDFTYQKNSVTGFSNKGLQKVRRNKNLEIPKQHNGITITE
                     IGDNAFRNVDFQSKTLRKYDLEEIKLPSTIRKIGAFAFQSNNLKSFEASEDLEEIKEG
                     AFMNNRIGTLDLKDKLIKIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGALHLMFIG
                     NKVKTIGEMAFLSNKLESVNLSEQKQLKTIEVQAFSDNALSEVVLPPNLQTIREEAFK
                     RNHLKEVKGSSTLSQITFNAFDQNDGDKRFGKKVVVRTHNNSHMLADGERFIIDPGKL
                     SSTMVDLEKVLKIIEGLDYSTLRQTTQTQFREMTTAGKALLSKSNLRQGEKQKFLQEA
                     QFFLGRVDLDKAIAKAEKALVTKKATKNGHLLERSINKAVLAYNNSAIKKANVKRLEK
                     ELDLLTDLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSD
                     TIGEGQKDAYGNPILNVDEDNEGYHTLAVATLADYEGLYIKDILNSSLDKIKAIRQIP
                     LAKYHRLGIFQAIRNAAAEADRLLPKTPKGYLNEVPNYRKKQVEKNLKPVDYKTPIFN
                     KDLPNEKVDGDRAAKGHNINAETNNSVAVTPIRSEQQLHKSQSDVNLPQTSSKNNFIY
                     EILGYVSLCLLFLVTAGKKGKRARK"
     gene            7266..7655
                     /locus_tag="VBC94_001683"
     CDS             7266..7655
                     /locus_tag="VBC94_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEO6979941.1"
                     /translation="MITQEMKEIINSQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGTYYEAAKKWAEGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEIT"
BASE COUNT         2585 a         1225 c         1508 g         2351 t
ORIGIN      
        1 ccttggctcg cattaatttc gggttttgga aaaggagtag caatcgctac tccttttttg
       61 ttttacacag gaaaagtatg aaggaaaagc cttttttctt ataaaaatta agcttgctta
      121 attttttaat ttttatattc tttacattat gatgaaaaca caagatatag tggtgatata
      181 tgttgaaaga cacaaaactt tgtgttagta tactgtttaa gcagatcctt aataaaggta
      241 accgacaaaa aagtttaacg tgacttaaca aaacagtggt tttgaagcaa gagttagctt
      301 cttaccagaa taaaaaaaca tcaagaagaa cattccaata gttgtgtgat tttaacaaaa
      361 atccagtcct cttgggaggc ttttttggtg cttaaaactc gcttaatttt aatatttagg
      421 agactagttt aatgcaatca tactatgatc gttctcaatc accacttgac tatgccttgt
      481 cagaaaaagc ttttcctatg cgttctgtta attggaataa gctaaacgat gacaaagact
      541 tagaggtatg gaacagagta actcaaaatt tctggttacc tgaaaagatc ccagtttcta
      601 atgaccttaa ttcttggaga acattggatg ctgattggca gcaattaatt actcggactt
      661 ttactggtct aaccttatta gattctgtgc aggctactgt tggtgatatt gctcagatta
      721 agcattctca gacagatcat gaacaagtta tttatgctaa ctttgccttt atggtagcta
      781 ttcatgcgcg atcttatgga acgatttttt caaccttatg cactagccaa caaattgagg
      841 aagcacatga atgggtagtg gatactgaaa gtctacaagc tcgatcacgt atacttattc
      901 ctttttatac tggtgacgat cctttgaaat ctaaggttgc tgcagccatg atgccaggat
      961 ttttgttata tggtggcttt taccttcctt tttacctttc agccagagga aaattaccaa
     1021 atacgtctga tattattcgt ttaattttac gagataaggt aattcataac tattacagtg
     1081 gctataagta tcaacagaaa gttgctaaat tatctgtaga aaagcaagcc gaaatgaaga
     1141 catttgtctt tgacctcctc tatcagttga ttgatttaga aaaagcctat ctttacgaac
     1201 tatatgatgg ttttgatttg gctgaagatg caatcagatt tagcatttac aatgctggta
     1261 aatttttaca aaatttagga tatgattctc catttacaga agaagagact cgtatatctc
     1321 ctgaggtttt tgcacaactt tcggcgcgtg cagatgaaaa tcatgatttc ttctcaggaa
     1381 acggctcttc atatattatg gggattacag aagaaacatt ggatgaagat tgggagtttt
     1441 aaatggatag tacagtgaag gtggtctatt tttcatcgaa atctaacaat acccatcgtt
     1501 ttgttcaaaa gttggcttgt tctaaccagc gtattccatc agatggtagt agtatattgg
     1561 ttactgagga ttatattttg attgtgccta cttacgctgg aggaggagat gataccaaag
     1621 gcgcagttcc aaaacaagtt gtacagtttt taaatgttcg acaaaatcgg gaacattgcc
     1681 aaggggttat ttcttctgga aataccaatt ttggtgacac ttatgccatt gctggaccta
     1741 ttattgctcg aaaattaaat gttcctttgc ttcatcaatt tgaattactg ggaacacaag
     1801 aagatgttac aagggtaaag gagttacttt gccaattcac tagaaaggat aaataagatg
     1861 acacaatcag atgcatatct ctcgttgaac gcgaagacac gctttagaga tcgcacaggt
     1921 aattatcatt ttacttcgga taaagaggct gttgaacaat atatgataga acatgttgaa
     1981 cctaatacga tggtgttcac atcactaatt gaaaagctag attatttggt ttctaataac
     2041 tactatgaat cggaccttct aaaacaatat aaccttgagt ttatttgcca aatttttgag
     2101 catgcatacg ctaagaaatt tgcttttcta aattttatgg gggctttaaa attttataat
     2161 gcttatgctc ttaaaacaga agacaatcgt tattatttgg aacactatga agatcgtgtt
     2221 gtaatgaatg ctctgttttt agctgctggt gatgaaaaag ctgcctatga cctagtagat
     2281 gacatgctag caaatcgctt tcaaccagca acacctacct ttttaaatgc agggaaaaaa
     2341 cgtcgtggtg aatatatctc ttgctatttg ttgcgcattg aagacaatat ggaatctatt
     2401 tctcgtgcta ttagtacaag ccttcagtta tcaaaacgag gtggtggggt agcactttgc
     2461 ttaaccaact tacgggaatt tggtgctcca ataaaaggta ttaaaaatca agctactggt
     2521 attgtacctg tcatgaaatt actagaagat tcgttttctt acgccaatca attaggacaa
     2581 agacaaggag ctggtgcagt ttacttacac gctcaccatc ctgaagtatt aacgttttta
     2641 gatactaaac gtgagaatgc cgatgagaaa attcgaatta aatctctttc cctaggactt
     2701 gttattcctg atattacttt tgaattggct aaggcaaata aagatatggc cttgttttca
     2761 ccgtatgaca ttgagcgagt atatggtaaa ccaatgtctg atatctctat cacagaagaa
     2821 tatgaaactt tattagccaa tgcggatatt cgtaaaacct ttattagtgc acgaaaacta
     2881 tttcaaacga tagcagagct tcattttgaa tctggttatc cttatatctt atttgaagat
     2941 actgtaaatg ctaaaaaccc tcacaaaaaa gagggacgaa ttgttatgtc taatttatgt
     3001 tctgagattg cacaagttaa tacagctagt caattttcag aagacctgac tttcactaag
     3061 gttggacatg atgtttgttg taatttaggt tctattaata ttgcgcgtgc tatggatcaa
     3121 gcggcggatt ttgaaaaatt aattgctaat agcattagag cccttgatcg tgtatctcga
     3181 acttctgatt tagatagtgc tccatctatt aaaaaaggta atgcagctaa tcatgctgtt
     3241 ggtttaggtg ctatgaattt gcatggattt ttggcgacta accatattta ttacgattct
     3301 caagaggcta ttgattttac agattgcttc ttttacgcta tggcttatta tgcctttaaa
     3361 gcttctaacc atttagcaaa agaaaaagga acctttgagg gcttctcgga atcaagttat
     3421 gctgatggca gttatttcta tcaatatacc gaacaaaact ttgagccaaa aacacagcgt
     3481 gttaaaaatc tactagctga gtatggtctg actctaccaa gccaagagga ttggagaaag
     3541 cttgtgcaaa gtattaaaga gataggatta gctaatgcgc atttattagc tgttgctccg
     3601 acagggtcaa tcagttatct ttcttcttgt actccgagcc ttcaaccggt tgtatcacct
     3661 gtcgaagtac gcaaggaagg agcactgggg agggtttatg tacctgctta taagattgat
     3721 gcagataatt atgtctacta caaaaaagga gcttatgaag tgggatctga ggcgattatc
     3781 aatattgcag ctgccgctca aaaacacatt gatcaagcta tttcgttaac gcttttcatg
     3841 acagatcaag caactacgcg agatttaaat aaagcctata ttcaagcatt taaacaaaaa
     3901 tgtgcctcta tttattatgt acgagtgaga caggacatcc tagaaggtag cgagagttat
     3961 gatgatatgc tggatgattt cacttcatcg gacttagaag actgtcaatc ctgcatgatt
     4021 taactaaata tactcaatca gaagattaag agaaatatat tggcttttag aaaggcttta
     4081 ttgtaatgac aaaaaaacat cttaaaacgc ttgccttggc acttactaca gtatcagtag
     4141 tgacatacag ccaggaggta tatggattag aaagagagga atcggtcaaa caagaacaaa
     4201 cccagtcagc ttcagaagat gattggttcg aagaagataa tgagaggaaa acaaatgttt
     4261 ctaaagagaa ttctactgtt gatgaaacag ttagtgattt attttctgat ggaaatagta
     4321 ataactctag ttctaaaacc gagtcagtgg taagtgaccc taaacaagtc cccaaagcaa
     4381 aaccagaggt tacacaagaa gcaagcaatt ctagtaatga tgctagcaaa gtagaagtac
     4441 caaaacagga tacagcttca aaaaaggaaa ctctagaaac atcaacttgg gaggcaaaag
     4501 atttcgtaac tagaggggat actttagtag gtttttcaaa atctggaatt aataagttat
     4561 ctcaaacatc acacttggtt ttaccaagtc atgcagcaga tggaactcaa ttgacacaag
     4621 tagctagctt tgcttttact ccagataaaa agacggccat tgcagaatat acaagtaggc
     4681 taggagaaaa tgggaaaccg agtcgtttag atattgatca gaaggaaatt attgatgagg
     4741 gagaaatatt taatgcttac cagttgacta agcttactat tccaaatggt tataagtcta
     4801 ttggtcaaga tgcttttgtg gacaataaga atattgctga ggttaacctt cctgagagtc
     4861 tcgagactat ttcagactat gcttttgctc acatgtcttt aaaacaagta aagttaccag
     4921 ataacctaaa ggtcattgga gaattagctt tttttgataa tcagattggt ggtaagcttt
     4981 acttgccacg tcacttgata aaattagcag aacgcgcttt caaatctaat cgtattcaaa
     5041 cagttgaatt tttgggaagt aagcttaagg ttataggaga agcaagtttt caagataata
     5101 atctgaggaa tgttatgctt ccggatggac ttgaaaaaat agaatcagaa gcttttacag
     5161 gaaatccagg agatgaacat tacaacaatc aggttgtatt gcgcacaagg acaggccaaa
     5221 atccacatca acttgcgact gagaatactt acgtcaatcc ggacaaatca ttgtggcgtg
     5281 caacacctga tatggattat accaaatggt tagaggaaga ttttacctat caaaaaaata
     5341 gtgttacagg tttttcaaat aaaggcttac aaaaggtaag acgtaataaa aacttagaaa
     5401 ttccaaaaca acacaatggt attactatta ctgaaattgg tgataacgct tttcgcaatg
     5461 ttgattttca aagtaaaact ttacgtaaat atgatttgga agaaataaag ctcccctcaa
     5521 ctattcggaa aataggtgct tttgcttttc aatctaataa cttgaaatcc tttgaagcaa
     5581 gtgaagattt agaagagatt aaagagggag cctttatgaa taatcgtatt ggaactctag
     5641 acttgaaaga caaacttatc aaaataggtg atgctgcttt ccatattaat catatttatg
     5701 ccattgttct tccagaatct gtacaagaaa taggacgttc agcttttcga caaaatggtg
     5761 cgcttcacct tatgtttatc ggaaataagg ttaaaacaat tggtgaaatg gcttttttat
     5821 ccaataaact ggaaagtgta aatctctctg agcaaaaaca attaaagaca attgaggtcc
     5881 aagctttttc ggataatgcc cttagtgaag tagtcttacc gccaaattta cagactattc
     5941 gtgaagaggc tttcaaaagg aatcatttga aagaagtgaa gggttcatct acattatctc
     6001 agattacttt taatgctttt gatcaaaatg atggggacaa acgctttggt aagaaagtgg
     6061 ttgttaggac acataataat tctcatatgt tagcagatgg tgagcgtttt atcattgatc
     6121 caggtaagct atcttctaca atggtagacc ttgaaaaggt tttaaaaata atcgaaggtt
     6181 tagattactc tacattacgt cagactactc aaactcagtt tagagaaatg actactgcag
     6241 gtaaagcgtt gttatcaaaa tctaacctcc gacaaggaga aaaacaaaaa ttccttcaag
     6301 aagcacaatt tttccttggt cgcgttgatt tggataaagc catagctaaa gctgagaagg
     6361 ctttagtgac caagaaggca acaaagaatg gtcatttgct tgagaggagt attaacaaag
     6421 cggtattagc ttataataat agtgctatta aaaaagctaa tgttaagcgc ttggaaaaag
     6481 agttagactt gctgacagat ttagtcgagg gaaaaggacc attagcgcaa gctacaatgg
     6541 tacaaggagt ttatttatta aagacgcctt taccattgcc agaatattat atcggattga
     6601 acgtttattt tgacaagtct ggaaaattga tttatgcact tgatatgagt gatactattg
     6661 gcgagggaca aaaagatgca tatggtaatc ctatattaaa tgttgacgag gataatgaag
     6721 gttatcatac cttggcagtt gccactttag ctgattatga aggtctttat attaaagata
     6781 ttttaaatag ttcccttgat aagattaaag caatacgcca gattcctttg gcaaaatatc
     6841 atagattagg aattttccaa gctatccgaa atgcagcggc agaagcagac cgattgcttc
     6901 ctaagacacc taaggggtac ctaaatgaag tcccaaatta tcgtaaaaaa caagtggaga
     6961 aaaatttaaa accagttgat tataaaacgc cgatttttaa taaggattta cctaatgaaa
     7021 aggtagacgg tgatagagcg gctaaaggtc ataatataaa tgcggagact aataattctg
     7081 tagctgtaac accaataagg tccgagcagc aattacataa gtcacagtct gatgtaaatt
     7141 tacctcaaac aagttctaaa aataatttta tatacgagat tctaggatac gttagtttat
     7201 gtttgctttt cctagtaact gctgggaaaa aaggaaaacg agcaagaaaa taaggagaaa
     7261 ttaatatgat tacacaagaa atgaaagaga ttattaatag tcagttagca atggtcgcga
     7321 cggttgatgc taaaggtcaa cccaatattg gcccaaaacg ttctatgcgt ctctgggatg
     7381 ataaaacgtt tatttataat gaaaatacag atggtcaaac tcgtattaac attgaagata
     7441 atgggaaaat tgaaattgcc tttgttgatc gtgaacgtct tttaggttac aggtttgtgg
     7501 gaactgctga aattcaaaca gaaggtactt attacgaagc agcgaaaaaa tgggcagaag
     7561 gacgcatggg ggttccgaaa gcagttggta ttattcatgt cgagcgtatt tttaatttac
     7621 aatctggtgc taatgcaggt aaagaaatca cttaatataa aaaagcctc
//
LOCUS       DATROQ010000086         7611 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.087, whole genome shotgun sequence.
ACCESSION   DATROQ010000086 DATROQ010000000
VERSION     DATROQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.087"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            88..2325
                     /gene="metE"
                     /locus_tag="VBC94_001684"
     CDS             88..2325
                     /gene="metE"
                     /locus_tag="VBC94_001684"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEO6979942.1"
                     /translation="MVKVSNLGYPRLGEQREWKQAIEAFWAGNLEQKDLEKQLKQLRI
                     NHLKKQKEAGIDLIPVGDFSCYDHVLDLSFQFNVIPKRFDEYERNLDLYFAIARGDKD
                     NVASSMKKWFNTNYHYIVPEWEVETKPHLQNNYLLDLYLEAREVVGDKAKPVITGPIT
                     YVSLSSGIVDFEATVQRLLPLYKQVFQDLIDAGATYIQIDEPIFVTDEGELLVDIAKS
                     VYDFFAREVPQAHFIFQTYFESAVCLDKLSKLPVTGFGLDFIHGRAENVAAVKQGLFR
                     EKELFAGIVNGRNIWAVNLEETLALLEEIGPFVKRLTLQPSSSLLHVPVTTKYETHLD
                     PVLKNGLSFADEKLKELELLASAFDGNKTKGYHEALSRFSALQAADFRHVALESLAEV
                     RLERSPYKLRQALQAEKLQLPILPTTTIGSFPQSPEIRKKRLAWKRENLSDSDYKDFI
                     KTEIRRWIAIQEDLDLDVLVHGEFERVDMVEFFGQKLAGFTTTKLGWVQSYGSRAVKP
                     PIIYGDIKHIQPLSLEETVYAQSLTKKPVKGMLTGPITITNWSFERDDISRSDLFNQI
                     ALAIKDEIQLLEQSGIAIIQVDEAALREGLPLRQQKQQAYLDDAVAAFKIATSSVKDE
                     TQIHTHMCYSKFDEIIDSIRSLDADVISIETSRSHGDIIESFETAVYPLGIGLGVYDI
                     HSPRIPTKEEIIVNIQRSLKCLSKEQFWVNPDCGLKTRREAETIAALEVLVSATKEVR
                     QQLDN"
     gene            2370..4214
                     /locus_tag="VBC94_001685"
     CDS             2370..4214
                     /locus_tag="VBC94_001685"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEO6979943.1"
                     /translation="MSKFLEKLKTDILVADGAMGTLLYTYGLDTCHESYNVTHPEKVL
                     AIHQAYIEAGADVIQTNTYGAQRHRLKNYGLEDQVVSINQAAVNIAHQATLGKETFIL
                     GTVGGFRSQRQCDLTLDNIVEETLEQVEALLVTGQLDGLLFETYYDIEEITTVLKIVR
                     EMTDLPIITNISLHEAGVTSNGKPIVEALSQLVMLGADVIGLNCHLGPYHMIQSLKQV
                     PLFAQSYLSVYPNAGQLSLDGENSQYQFSQNSEYFGKSAELLVAEGVRLIGGCCGTTP
                     DHIRAVKRSIRGLKPIERKVVTPIIPVKDFVRRIRRTDTLVDKVKKEVTIIAELDPPK
                     HLDIVQFQKAIRAIDQKGIAAITLADNSLSNTRICNLSIASLLKDEISTPFLLHIACR
                     DHNLIGLQSRLLGMELLGFNHILAITGDPTKLGDFPGATSVYDVTSFKLLSLIKQLNQ
                     GLSYSGASLRRPTDFTVAAAFNPNVKNLTRTVKLIEKKVASGADYFMTQPIFDHSVLR
                     ELADLTKTVEQPFFIGIMPITSYNNAVFLHNEVPGIKLSESFLSALEKVKDDKEACLT
                     LALNESKSLIDEALNHFNGIYLITPFLRYDLTLELIDYIQKKQVRKSS"
     gene            4561..5268
                     /locus_tag="VBC94_001686"
     CDS             4561..5268
                     /locus_tag="VBC94_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEO6979944.1"
                     /translation="MVCVDFIDQKVYSEAEGLFVSDLDLGIKGLIRARYPFALDSDFI
                     SYGNLSHYCMNYLDEIVQRANQKNEKIKYNVTTLMKEEEKPFNVEERLNKQATIGQRI
                     ADDVARFGGSWTFIIVFVSIMAIWMLVNIMKPFGIQFDPYPFILLNLALSTIAAIQAP
                     LIMMSQNRAADYDRLQARNDFNVNKTSELEIRLLHEKIDHMVQQDQFELLEIQKLQTE
                     MLVSLGNQLAQLKQLQK"
     gene            complement(5433..6518)
                     /locus_tag="VBC94_001687"
     CDS             complement(5433..6518)
                     /locus_tag="VBC94_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO6979945.1"
                     /translation="MTTNVMANYSYGEDCFKEIPSVLATYRIQSIAFVGGKRALDSSE
                     AEVRSILEEANIKVTGSFVYGTDSTQSNIDKLVANPQVQAADAILGFGGGKALDTAKM
                     VAKELGKNSFTIPTICSNCSAGTAIAVVYNDDHSFLRYGYPESPLHIFINTRIIAQAP
                     SKYFWAGIGDGISKAPEVERATLEAKTNKLPHTAVLGQAVALSSKEAFYQFGEQGLKD
                     VEANLASRAVEEIALDILISTGYASNLVNQPDFYYNSCHAHAFYYGTTAIQRQGEFLH
                     GVVVAFGVLVLHAYFNELEELEKVARFNKSLGLPTTLADVSLSEKDIPKIVEIAMTTN
                     EYKNTPFDPKMFAQAILAADAFGQTLA"
     gene            complement(6719..7258)
                     /locus_tag="VBC94_001688"
     CDS             complement(6719..7258)
                     /locus_tag="VBC94_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEO6979946.1"
                     /translation="MKIAIIGYSGSGKSTLARKLGNYYNCNVLHLDSIHFAPNWEERK
                     YDDMIDDVSSMLEKRTWIIEGNYKKLLYQERLADADEIIFFDFNRFNCLWRAFKRYCK
                     FRGKTRPDMANGCPEKLDFEFISWILKDGRSDKQKSNYKQVVEDYPQKIKVLKHQRDL
                     DQYLKELPVASNTDPLKSL"
BASE COUNT         2441 a         1243 c         1463 g         2464 t
ORIGIN      
        1 agctattgat attatagttt tcatggacta aaataattca taaaactaaa agggttagtt
       61 attagctata acttgatgga ggaaattatg gtaaaagttt caaatttagg gtatccacgt
      121 cttggtgaac agcgcgaatg gaagcaagcg atcgaagctt tctgggcagg gaatcttgaa
      181 caaaaagatt tagaaaaaca actaaaacaa ttacgtatca atcatttaaa gaaacaaaaa
      241 gaggcaggta ttgaccttat tccagtgggg gatttttctt gttatgatca tgttttggat
      301 ttgtcatttc aattcaatgt aatcccaaag cgtttcgatg agtatgagag gaatttagac
      361 ctttattttg ctattgcaag aggtgacaaa gataatgtcg catcatctat gaaaaagtgg
      421 tttaatacca actaccacta catagtccca gaatgggagg ttgagactaa acctcacttg
      481 cagaataatt acttacttga tctttatcta gaagctaggg aagtagttgg tgataaagca
      541 aagccggtta tcactggtcc aataacctat gtttccttat catcaggaat tgtcgacttt
      601 gaagcgactg ttcagcggtt attaccactt tataagcagg tctttcaaga tctgatagat
      661 gcaggcgcca cctatattca gattgatgag ccgatatttg taactgatga aggtgaactt
      721 ttagtagata tagctaagtc tgtctatgat ttttttgcaa gagaagtacc acaagcccac
      781 ttcatctttc aaacctactt tgaatcagca gtctgtttag ataaactctc taagctgcca
      841 gtaacgggat ttggccttga ttttatacat ggtagggcgg aaaatgtagc tgctgttaag
      901 caaggtctat tccgcgaaaa agaattattt gcaggaattg ttaatggtcg aaatatctgg
      961 gcagtaaatt tagaagaaac gttggcttta ttggaagaga taggtccctt tgttaaacgt
     1021 ttgacccttc aaccttcttc aagtctttta catgtaccgg tgacgactaa atacgaaaca
     1081 catttagacc ctgtgttaaa gaatggctta tcatttgctg atgaaaaact aaaggaatta
     1141 gaactattag cgagtgcttt tgatggtaat aaaacaaagg gatatcacga agctttatct
     1201 cgtttttcag ctcttcaagc tgctgatttt cgtcatgtag cgttggaatc attagcagaa
     1261 gtaaggcttg aacgaagtcc gtataaatta cgccaagctt tgcaagctga aaaattacag
     1321 ttaccgattt taccaacaac aactattggg tcctttcctc aatcacctga aattaggaag
     1381 aaacgccttg cttggaaaag agaaaatcta tctgactcag attataaaga tttcataaaa
     1441 actgaaatta gaagatggat tgctattcaa gaagatcttg atcttgatgt gttagtacat
     1501 ggcgaatttg agcgcgtgga tatggttgaa ttttttggtc aaaagttagc tggttttacg
     1561 acaaccaaat taggctgggt acagtcttat ggttcaaggg cggtcaaacc acctatcatt
     1621 tatggtgata tcaaacatat tcaaccctta agccttgaag aaacggttta tgcccaaagc
     1681 ttgactaaga aacctgttaa aggcatgttg acaggtccta ttactataac gaactggtca
     1741 tttgagcgag atgatatcag ccgatctgat ctttttaatc aaattgcttt ggctataaaa
     1801 gatgagattc aacttttgga acaatcaggt attgctatta tacaagtgga tgaagcagcc
     1861 cttcgagaag gcttaccctt acgccagcaa aagcaacagg cttacttaga tgatgctgtt
     1921 gcggccttta aaattgcaac ttcatctgtg aaagatgaga cacaaattca tacacatatg
     1981 tgttattcaa aatttgacga aattattgat tctatccgtt cactagatgc agatgttatt
     2041 tctattgaaa cgagtagaag tcatggggac atcattgaaa gttttgaaac agcagtttat
     2101 cctctaggaa ttggtctggg tgtttatgat attcattctc ctcgcatacc tactaaggaa
     2161 gaaattattg tcaatattca acgatcacta aaatgtctat caaaagagca attttgggta
     2221 aaccctgact gtggcttaaa aacacgtcgt gaagcagaaa caattgctgc cttggaggtt
     2281 cttgtttcag ctaccaaaga ggttcgtcag caattagata attaataggt taatcagtaa
     2341 aggattgcca tgagggagga gtaaagaaga tgtccaagtt tttagagaaa ttaaaaacag
     2401 atattctagt agctgacggt gcaatgggaa ctctccttta tacttatggg ctagatactt
     2461 gtcatgaatc ctacaatgta acacatccag aaaaggttct agccattcat caagcttata
     2521 ttgaagcggg tgcagatgtt attcagacga atacctatgg ggctcaacgt catcgattaa
     2581 agaactacgg tttggaagat caggttgtct ctatcaatca agcagcagtt aatatagctc
     2641 atcaagcgac tttgggtaaa gaaacattta ttttaggtac tgttggaggt tttcgttctc
     2701 aaagacaatg cgatttaaca ttggataata ttgtagaaga aactttggaa caagttgagg
     2761 ctttattggt gacaggacag ctagacggat tattgtttga aacttattat gatattgagg
     2821 agataacgac agttttaaaa attgtgaggg agatgactga tctaccaatt attactaata
     2881 tttctcttca tgaagcaggg gtaacatcaa atggaaagcc tattgtagag gcgctcagcc
     2941 aattagttat gctaggtgca gatgtgattg gcttaaattg tcaccttgga ccctatcata
     3001 tgatccaatc attgaagcaa gttcctttat ttgcccagtc ttatttgtca gtttatccta
     3061 atgcaggtca gttatcttta gatggtgaga atagtcaata ccagtttagt cagaattcag
     3121 agtattttgg aaaatctgca gaacttctcg tagcagaagg agttaggctt attggtggtt
     3181 gttgtgggac aactccagat catattagag ctgtgaaacg atctattaga ggattgaaac
     3241 ctattgaacg aaaggttgtt actcctataa tacctgtaaa agattttgtt aggcgtatca
     3301 gacgaacaga tactctagtg gataaggtaa aaaaagaagt aacaattatc gcagaattag
     3361 atcctccaaa acatttagat atcgttcagt ttcaaaaggc tatacgagcg attgaccaaa
     3421 aaggtattgc agcaattact ttagcagata attcactatc taatacacgt atttgtaatt
     3481 taagtatagc tagcttgctt aaagacgaaa ttagtacacc ctttttacta catatagctt
     3541 gtcgtgacca taatttaatt ggtttacagt ctagactgtt gggcatggaa ttattaggtt
     3601 ttaaccatat tttagccata acaggagatc cgactaaact tggtgatttc ccaggagcga
     3661 ctagtgttta tgatgtgact tcctttaaac tcctttctct gattaaacaa cttaatcaag
     3721 gtttatctta tagtggtgct agcttgagac gtccaacaga ttttacagtt gctgctgcct
     3781 ttaatcctaa tgtgaaaaac cttactcgaa ccgtaaaatt gattgagaaa aaagtagctt
     3841 ctggtgctga ttattttatg acacaaccaa tatttgatca ctctgtgtta agggaattag
     3901 ctgatcttac taaaacagtt gaacaaccct tttttatcgg aattatgcca ataacaagtt
     3961 ataataacgc tgtttttcta cacaatgaag ttcctggtat taaactttct gagtcatttt
     4021 tatctgctct tgaaaaggta aaggacgaca aagaagcttg tttaactttg gccttaaacg
     4081 agagtaagtc cttgattgat gaagccttga accattttaa tggtatctat ttgattactc
     4141 cctttttacg ctatgatctg actcttgagt taattgacta tatacaaaaa aagcaagtaa
     4201 gaaagtcctc ttaactttct tactttttag ttggtcgttt attattataa taaaagttac
     4261 gtaccaaaac agaggttaaa ctagattttt aagccacttt ctatactaag ataaattagt
     4321 taatttaagt tttctttaag tttatagact tagaataaag cgttattttg atgggtagca
     4381 taaaatcaag gcataatata gatgtcagcg atttttttag actaacgaaa taacgatgta
     4441 aagcttaata ggggtgtatc gaatactcac ttatatagta ccaaaagcct atagcctgtt
     4501 ggctagcttg accgtattca aatgttgaat aaatcttggt tgagaaaggg aggtataaaa
     4561 atggtatgtg tcgattttat tgatcaaaaa gtttatagtg aagcagaagg tctatttgtt
     4621 tccgatttag acttaggcat taaaggttta ataagagcac gttatccttt cgctctggat
     4681 agtgacttta tttcgtatgg gaatttaagt cattattgta tgaattatct tgatgaaatt
     4741 gttcagcgag cgaatcagaa aaatgagaag ataaaatata atgtgactac cttgatgaaa
     4801 gaagaagaga agcccttcaa cgttgaagag cgtttgaaca agcaagcaac tatcggtcaa
     4861 cgaattgctg atgatgttgc acgttttggc ggatcttgga cttttattat cgtttttgta
     4921 tccattatgg ctatttggat gttggttaat atcatgaagc cttttggtat tcaatttgat
     4981 ccttacccat ttatcctgtt aaacttagct ttatcaacga ttgctgcgat ccaagcacca
     5041 cttattatga tgagtcaaaa tcgtgctgct gattatgatc gtttgcaagc tcgaaatgac
     5101 tttaatgtca ataaaacctc tgagttagaa attagattat tgcacgaaaa aattgaccat
     5161 atggtacaac aagatcagtt tgaattgtta gagattcaaa agttacagac agaaatgcta
     5221 gtatcattgg gaaatcagtt agcacaattg aaacaactac aaaaataatc ttttagcagt
     5281 tatttaaaaa tactgataca atactaagaa tttaaattaa tactgcaacc tcctataatg
     5341 gagagcagta tttttacttt attaataaca aaaacccctt agatgatgtg tctaagggat
     5401 ttttgtatcg gaataatcga gttggtgaat tattaggcta gtgtctgtcc gaaagcgtct
     5461 gctgctaaaa tagcttgagc aaacattttg ggatcgaaag gggtattttt gtattcatta
     5521 gtagtcattg caatttcaac aatttttgga atatcttttt ctgataaaga gacgtcagct
     5581 agagttgttg gcaaacctag tgacttatta aaacgagcta ctttttcaag ttcttctagt
     5641 tcgttgaaat aagcatgtaa aactaacaca ccaaacgcaa ctacaacgcc atgtaagaat
     5701 tcaccttgtc tttgaatagc ggttgtacca tagtaaaatg catgcgcgtg acaagaattg
     5761 tagtagaaat caggttgatt aactaaatta gaagcatagc cagttgagat taagatatca
     5821 agcgcaattt cttcaactgc acgcgaagct aaattagctt caacgtcttt tagaccttgt
     5881 tcaccaaatt gataaaaagc ttcctttgaa gacagagcga ctgcttgtcc taacactgca
     5941 gtatgtggta gtttattggt cttagcctct aaggtagcac gttctacttc aggggctttt
     6001 gaaataccgt ccccaatacc agcccaaaaa tatttgcttg gtgcctgtgc aatgatccgt
     6061 gtattgataa aaatatgaag tggagactcg ggatagccgt atcttaagaa agaatggtca
     6121 tcattatata caactgcaat ggcagtacct gcggagcaat tagaacaaat agttgggata
     6181 gtaaagctat ttttacctaa ctcttttgct accattttag ctgtgtctaa agcttttcca
     6241 ccgccaaaac ctaagatagc atcagcagct tgaacttgtg ggttggctac taacttgtca
     6301 atgttagatt gtgttgaatc cgttccatag acaaagcttc ctgtgacttt aatatttgct
     6361 tcctctaaaa tagagcgaac ctcggcttct gagctgtcta gggcacgttt gccacccacg
     6421 aaagcaatgc tttgaatacg gtaagttgcc aaaacgctag gaatttcttt gaagcaatct
     6481 tctccgtagc tataatttgc cattacattt gttgtcattc agtgtcctcc tctattccaa
     6541 aggtcgatta gagacagcta ttttctaaaa gtgaatagtt ttttgtctct tgaccaagtg
     6601 attatcatat tatagcagaa tattcagaaa attggaacat gaaaaataat ttaattttca
     6661 agaataagaa tagaaaaggt aaagcgagat atacgagtaa atactgtcag tataataatt
     6721 ataagctctt gagagggtct gtattggatg caacagggag ttcttttagg tattgatcaa
     6781 gatcgcgttg gtgttttagc acctttattt tttgagggta gtcctcaaca acttgtttat
     6841 agtttgattt ttgtttgtca gagcgaccgt ctttaagaat ccaactgata aattcaaaat
     6901 ctaatttttc cgggcatcca ttggccatat caggtctagt cttaccacga aacttacagt
     6961 aacgtttgaa tgctctccac aagcagttga agcgattaaa gtcaaaaaaa ataatctcat
     7021 ctgcatctgc tagacgttct tgatatagca gttttttgta gttaccttct ataatccatg
     7081 ttcttttttc taacatgctg ctgacatcgt caatcatgtc atcatatttt ctctcttccc
     7141 aatttggggc gaagtgaata ctatcaaggt gtaatacatt gcaattatag taatttccca
     7201 attttctcgc taaagtggac ttacctgacc cactatatcc gattattgct attttcatat
     7261 actacctccg ttaaattgtt tacttgaagt taatgctgta cttaggattt acttatttta
     7321 taagggttat tcactttttt agattaatat atttttgata taagttatcc atacacatgg
     7381 taacataatt accagtaaat gtaatctaga tatcaatact atggttgacg atattgttaa
     7441 cttttgggtt caaaaaattg gggtaaaaat catcggaggg aactgccaaa caaactcaca
     7501 gtttaaaata ctaagtaata aaaagtcttg aatttattat gtttaaggct ttttaagcgt
     7561 ctaaaatact tagtctagta tgaatttcta aacagaaagt aaaaataatg a
//
LOCUS       DATROQ010000087         7456 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.088, whole genome shotgun sequence.
ACCESSION   DATROQ010000087 DATROQ010000000
VERSION     DATROQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.088"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            38..376
                     /locus_tag="VBC94_001689"
     CDS             38..376
                     /locus_tag="VBC94_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO6979947.1"
                     /translation="MRRVSVSHDLKELLLELKFQQERMLKGLHIKNTDQLIFYDYRFG
                     MISNDAVNKFLASSLEKLEIESKMSSTGARHTYGSYLLANGVDIWAVAKLMGHKDIKQ
                     LIETYGHLLL"
     gene            complement(512..582)
                     /locus_tag="VBC94_001690"
     tRNA            complement(512..582)
                     /locus_tag="VBC94_001690"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(548..550),aa:Thr,seq:ggt)
     gene            complement(653..1487)
                     /locus_tag="VBC94_001691"
                     /pseudo
     CDS             complement(653..1487)
                     /locus_tag="VBC94_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018434.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            1798..2052
                     /locus_tag="VBC94_001692"
     CDS             1798..2052
                     /locus_tag="VBC94_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEO6979948.1"
                     /translation="MSYEQEFLKDFEEWLQSQISINQMAMDSAKKVLEEDKDERAADA
                     YIRYESKLDAYRFLQGKFNNYHNQKSFHDLPDGLFGQRHY"
     gene            2161..2472
                     /locus_tag="VBC94_001693"
     CDS             2161..2472
                     /locus_tag="VBC94_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979949.1"
                     /translation="MAKRIKPNKKRELLKAKGINWKDVRLSQTLQEVASPKKKAPEAP
                     KAVDLLEGLTANKQDVLKEAGLVSLEAFAKVSEADVLALKGIGPAAIKQLVDNGAVFA
                     K"
     gene            2740..3309
                     /locus_tag="VBC94_001694"
     CDS             2740..3309
                     /locus_tag="VBC94_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6979950.1"
                     /translation="MQILEDFDGKALPKLETDRLILRQRTVGDVPAMFDYVCLEEVAY
                     PAGLSPIASLEDEYDYFENRYYQNLEKAKLPSGYGITVKGSDRIIGSCDFNHRHEDDV
                     FEIGYMLHPDYWGHGYMTEAVAALIEVGFTLLNLHKIEIRCYDYNKQSRRVAEKLGFT
                     LEATIRDRKDNQGNRCVNLIYGLLRSEWE"
     gene            3407..3991
                     /locus_tag="VBC94_001695"
     CDS             3407..3991
                     /locus_tag="VBC94_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6979951.1"
                     /translation="MIKILGEIVDNQLPVVETNRLLLRQRKLEDAKEIFEFVKLDEVT
                     YPAGFPAVKSLEEEITYIQEIYPTNLEKEKLPSGYAITLKGDDKVIGSVDFNHRHEDD
                     IFEIGYLLHPDYWGQGIVPEAASVLVEIGFTLLGLHKIELGCYDYNKQSQAVARKLGF
                     TLEANIRDRRDAQGQRCGDMRFGLLRSEWEKKRR"
     gene            3988..4554
                     /locus_tag="VBC94_001696"
     CDS             3988..4554
                     /locus_tag="VBC94_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6979952.1"
                     /translation="MNIIIIGAQASGKMTIGQEIAKQTGMTLFLNHDSIDFVLRFMPW
                     SPDSIALTESIRFKFFETFAKTGQEMIFTIVIDFNDSRDVAFLEKIQTVFQSHNQEVL
                     FVELETELSERLKRNRTENHLKHKPSKRDIKWSENDICSTMDYAIFNPEVAPEALTYY
                     YKINNTCLTATETAYLIIQKINQIKEKN"
     gene            4554..7205
                     /locus_tag="VBC94_001697"
     CDS             4554..7205
                     /locus_tag="VBC94_001697"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEO6979953.1"
                     /translation="MSKELSPKYNPAEVEAGRYQTWLDQDVFKPSGDAEAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQG
                     ISRYDLGREKFLDKVWEWKDEYAATIKSQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYNKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGQNVILPIINKPIPIVADEHADPEFGTGVVKIT
                     PAHDPNDFAVGQRHNLPQVNVMNDDGTMNELADEFNGMDRFEARKAVVAKLESLGNLV
                     KIEKMTHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FMSWMENVHDWVISRQLWWGHQIPAWYNVNGEMYVGEDAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPDTEAADFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFSNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDQARENVEKVVNSQVGNVTDRWILHNLNETVGKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDEKVVTRSVLLYTLDQI
                     LRLLHPIMPFVTEEIFGQYADSSIVTAAYPTVNPAFENAAAHKGVESLKDLIRSVRNS
                     RAEVNVAPSKPITILVKTSDSELESFFKDNSNYIKRFTNPETLEISSAIATPELAMSS
                     VITGAEIFLPLADLLNVEEELARLEKELAKWQKELDMVGKKLGNERFLANAKPEVVQK
                     EKDKQADYQAKYDATQERIVEMQKLVK"
BASE COUNT         2399 a         1295 c         1532 g         2230 t
ORIGIN      
        1 aaaaaatgac tttacaaagc ctaaaacaag gacttcaata aggagagttt cagtaagtca
       61 tgatttaaaa gagttattgc ttgaactaaa atttcaacaa gaaagaatgc tgaaaggttt
      121 gcatataaaa aatacagatc aattgatttt ttatgactat aggtttggta tgatatcaaa
      181 tgatgctgtt aacaaattct tggcaagctc attggaaaaa ttagaaatag aatctaagat
      241 gtcatcaaca ggtgctagac atacttatgg aagttatttg ttggctaacg gagtagatat
      301 ttgggcggtt gcaaaattaa tggggcataa agatatcaaa caattaatag aaacatatgg
      361 tcatttgctt ctgtaagtgg aagaaaagga aaatgatcag cttttaaaaa ttcttaataa
      421 taaaggtagt ttttaaaatg actatcaaga acaaattaag aacaaaaata aaagaaaccc
      481 tattataata aggtttcact catatatttt ctgccaccac cgagaattga actcggaatt
      541 gagcattacc attgctctat tataccattt aactatagcg gctacttttc tatgatacta
      601 taagattttg acgaaatcaa gtatttcttt acttagaaaa atagtcgttt ttttagtcta
      661 attctatttg gagaaattta ctattttcaa tagctcttaa atggtgtgtt tcttgtggag
      721 agaggctaat aaggctatct ttttcaagga tatgtttctg atcaccaagg ataatttcaa
      781 gttttctttc caaaacattt attaattttg gatgagggca tcgttcattt ttcagtcccc
      841 ggctgatgat tttaatggta ttgaataaac cttcatcttt aatcattcct aacggggaca
      901 taagacttat ttttccaaat tgatattctg aactttaaaa actattttgt aagaataaat
      961 tttgccaatc atctattttt agaggagcaa cgttaacatt catagtatgt ctaagttgat
     1021 cgatcagctt ggcagtgtct tcatcttgat aagaaacatc atgtgtgagt aaacgtccac
     1081 caggttttag gacacgtaag tactctttta tggctttctc cttagctgaa ttgttaagca
     1141 tcgttaacat tgcttcgttg ataacgataa actattatca ggaaagggca gtctgagtgc
     1201 atttccctga ataacttcaa taaattcttc taattgggcc tttttgacat tttctttggc
     1261 cttttctatt actttactat ctagatcaag cgctgtaatg tgacaggaaa atgctttagc
     1321 gatatcgatg gaactggtac attgattaca agctacttct agaacacgac tgtcagcact
     1381 aaagtgtcct tttttgataa gccaattcgt tgcttggaca cctcctggtc ttaagcgttt
     1441 tttcctagtc gtgctaagaa ttgatgtcca atttctttat gtggcaataa gatcacctct
     1501 ttcttttaat agtaatatca ttttttctta attataacac atagatgcta agataacaag
     1561 gagtaatagt gattgaatgg tataagtgat gaattttaga gagcgttttc ttttaactag
     1621 gggtgtgatg ccaaaactag aatggcatag agaaccatac atctcaaaag gtcttcctta
     1681 gataaaacta gttgttttta tatctgtcag aaaaatcata atttattttt gatataatga
     1741 ttttttgagt atttaattta tatgttataa tgaaaaaaac taaaaaagag gaatatcatg
     1801 tcatacgaac aagaattttt aaaagacttt gaagagtggt tacaatcgca aataagtatc
     1861 aaccaaatgg caatggacag tgcaaagaaa gttttagagg aagataagga tgagcgagca
     1921 gcagatgcgt atattcgtta tgagagtaag ttagacgctt atcgcttttt gcaagggaaa
     1981 tttaataact atcataacca aaaatctttc catgatttgc ctgatggtct ttttggacaa
     2041 cgtcattatt aaaaggttta tcgctagaaa gcttgtcaaa taggcatttc tatgatacat
     2101 tgttatagta gtttttagat actatgatag atttcataaa tgaggagaaa gcattataca
     2161 atggcaaagc gtattaaacc gaataaaaaa agagagcttt tgaaagctaa agggatcaat
     2221 tggaaggatg ttcgactatc tcaaacttta caagaagtcg catcaccgaa aaaaaaggct
     2281 cctgaagcgc caaaggcagt cgatctttta gaaggtttaa ctgctaataa acaagatgtt
     2341 ttaaaagaag ctggccttgt cagccttgaa gcgtttgcga aagtaagtga agcagatgtt
     2401 ttagcactaa aaggtattgg tccagcagct attaaacagt tagttgataa tggtgctgtt
     2461 tttgcaaaat gatttgattc accttgcgaa agtagtctta ttttgctaga ataaattaac
     2521 ttagaggtgt tttgaaatcc catttgtcac aggaagcggt catactgaga aatccgcaca
     2581 aatgtcaaag cttgttgcta agtccaataa ttgaaacaaa acgagttgcg gatgttatca
     2641 tccgaatttg ggtggtaccg cggaaatcgt aatacgaaat tcgtccctgt cagaattgac
     2701 agggattttt tgtatttagg agtgaggaga ttgaagtata tgcagattct agaagatttc
     2761 gatggcaaag cattaccaaa actagaaacg gatcgactta tcttgcgtca gcgaacggtg
     2821 ggggatgtcc cagccatgtt tgattacgtt tgcttggaag aggttgccta tccagcaggg
     2881 ctcagtccaa tagcttcttt agaagatgag tatgattatt ttgaaaatcg ttactatcaa
     2941 aatctggaaa aggcaaaatt accatctggt tatggtatta ccgttaaagg aagtgaccgg
     3001 attatcggct cttgcgactt taaccaccgc catgaagacg atgttttcga gattggctac
     3061 atgcttcatc ctgactattg gggtcatggt tatatgacag aagctgtcgc agccttgatt
     3121 gaagtcggtt ttaccctcct caatctccac aaaatcgaaa tccgctgcta tgattacaat
     3181 aagcaaagcc ggcgcgtggc agaaaaacta ggcttcaccc ttgaagccac catccgtgat
     3241 cgtaaggata atcaaggtaa tcgctgtgtc aatttgatat atggtttgct gaggagtgag
     3301 tgggagtaat tattctactc ttcatagaag ttactagata gactaatttg gctgtgcagg
     3361 gtacttagct aactcatcaa taatatcgta gatatggagg agacaaatga ttaagatact
     3421 tggagaaatc gtagataacc agctacctgt tgtggaaacg aatcgtctgt tgttgcgtca
     3481 acgcaagtta gaagatgcta aggagatatt tgaatttgtc aaacttgatg aggtgactta
     3541 tccagcaggt tttccggctg ttaaaagctt ggaagaagag ataacctata ttcaagagat
     3601 ttatccaact aatcttgaaa aagaaaagtt accctctggt tatgctatta ctctaaaagg
     3661 cgatgataag gtaattggtt cagtagattt taaccatcgc cacgaagacg acatttttga
     3721 aattggctac ctgctccacc ctgattattg gggacaaggc attgtgccag aagcagcttc
     3781 agtccttgtg gagataggtt tcacactgct aggactacat aagattgagc tgggatgcta
     3841 tgactataat aagcaaagtc aggcggttgc gcgcaaatta ggttttactt tagaggctaa
     3901 tattcgtgat cgaagagatg cccaggggca acgttgtggc gatatgcgct ttggtttact
     3961 gcgaagcgag tgggagaaaa aaagaagatg aatattatta tcatcggagc acaagcttct
     4021 gggaaaatga caattggtca ggaaatcgca aaacagacag gcatgacctt attccttaac
     4081 catgattcta tagattttgt tttacgcttc atgccatggt ctccagatag tattgctcta
     4141 acggagtcta ttcgctttaa attttttgaa acgtttgcta agactggtca agaaatgatt
     4201 tttactattg taattgattt caatgattca agagatgtag catttctaga aaagattcaa
     4261 acagtttttc aatctcacaa ccaagaggta ttatttgtag aattggaaac agagctaagt
     4321 gagcgtctca aacgaaatcg cacagaaaat cacctcaagc ataagccttc aaaacgtgat
     4381 atcaaatggt cagagaacga tatttgttca accatggatt acgctatctt taatcctgaa
     4441 gtagccccag aagcgcttac atattattat aaaatcaata atacttgctt aactgctact
     4501 gaaacagcat atcttattat tcaaaaaata aatcaaatca aggagaaaaa ctaatgtcaa
     4561 aagaactatc accaaaatac aatcctgccg aggttgaggc aggccgttat caaacttggc
     4621 ttgaccaaga tgttttcaag ccatcagggg atgctgaggc taagccttat tctattgtga
     4681 ttccaccacc aaacgttacg ggtaaattgc acttgggtca cgcttgggat acaactctac
     4741 aagatatcat tattcgtcaa aagcgtatgc agggctttga tacactctgg cttccaggta
     4801 tggaccacgc tggtatagcc actcaagcta aagttgagga gcgccttcgt gagcaaggca
     4861 tttcacgtta tgatcttggt cgtgaaaaat tcctagataa agtttgggaa tggaaagacg
     4921 agtatgcagc aactattaaa tctcagtggg ggaaaatggg tctttcggtt gattatagcc
     4981 gtgagcgttt tacccttgat gagggacttt caaaagcggt aagaaaagtc tttgttgatt
     5041 tgtacaataa aggttggatc tatcgtgggg agttcatcat taactgggat ccagcagctc
     5101 gtacagccct ttcagatatt gaagttattc acaaggatgt tgagggtgct ttctatcata
     5161 tgaattatat gctggaagat ggctcacgcg ccctagaagt agcaacaact cgtcctgaga
     5221 ctatgtttgg agatgtggct gtagcggtaa atccagaaga tccacgttac aaggatttga
     5281 ttggtcaaaa tgttatcctt cctatcatca ataaacctat tccaatcgtc gctgatgaac
     5341 acgctgatcc agaatttgga acaggtgtgg ttaagattac gccggctcac gaccctaatg
     5401 acttcgctgt aggtcaacgc cacaacttac cacaagttaa cgttatgaac gatgatggga
     5461 ctatgaatga acttgcggat gaatttaatg gtatggatcg tttcgaagct cgtaaggcag
     5521 tagtcgctaa attagaatcg ttaggaaact tagtaaaaat tgaaaaaatg actcattctg
     5581 ttggtcattc agaacgaaca ggcgttgtgg ttgaaccacg tttatctact caatggttcg
     5641 tcaagatgga tcaattggct aagaatgcca ttgccaacca agatacagaa gacaaggtag
     5701 agttttatcc accacgattt aacgatactt ttatgagctg gatggaaaat gtccatgact
     5761 gggttatctc tcgtcaatta tggtggggac accaaattcc tgcatggtac aacgtcaatg
     5821 gtgagatgta tgttggagaa gatgccccag agggtgacgg atggacacag gatgaagacg
     5881 ttctagacac atggtttagt tctgctttat ggccgttttc aacaatggga tggccagaca
     5941 ccgaagcagc tgacttcaaa cgctacttcc caacgtcaac cttggtaact ggatacgata
     6001 tcatcttctt ctgggtatcg cgcatgattt tccaatctct tgaatttaca ggacgccaac
     6061 cattctcaaa tgttcttatt catggactca tccgtgatga agaaggacgt aaaatgtcta
     6121 aatcacttgg caatgggatt gatccgatgg atgtcattga gaaatatggt gcggatgctc
     6181 ttcgttggtt cctctcaaat ggttcagccc ctggtcaaga tgtgcgtttt tcatacgaaa
     6241 aaatggatgc ttcgtggaat ttcattaata agatttggaa catttcacgc tatatcctta
     6301 tgaataacga aggtttaacg ttggatcaag ccagagaaaa tgtggaaaaa gttgttaata
     6361 gtcaagttgg aaatgtaaca gaccgttgga ttctccacaa tttaaatgag actgtgggta
     6421 aagttactga aaactttgac aagtttgaat ttggtgttgc tggtcacatc ctttacaact
     6481 ttatctggga agagtttgct aattggtacg ttgagttaac aaaagaggtt ctttatagtg
     6541 acaatgaaga tgagaaagtt gttacacgtt ctgttcttct ttatacctta gatcaaatct
     6601 tgcgtcttct tcatccaatc atgccattcg tgacagaaga aatctttggt cagtatgcag
     6661 atagctcaat cgtaacagct gcttacccta cggttaaccc agcctttgaa aatgctgctg
     6721 cccacaaggg agttgaaagc ttgaaggact tgattcgttc cgtgcgtaat agccgagcag
     6781 aggtcaatgt tgctccatct aaaccaatca ctatcttggt taagacaagt gacagcgaac
     6841 tcgaaagctt cttcaaagac aatagtaact acatcaaacg tttcacaaac ccagaaacac
     6901 ttgaaatcag ctcagctatt gccacaccag aacttgccat gtcatcagtc atcacaggag
     6961 ctgaaatctt cctcccactt gctgacctcc tcaacgtaga agaagagtta gcacgacttg
     7021 aaaaagaact agccaaatgg cagaaagaac tggacatggt aggcaaaaaa ctcggcaacg
     7081 aacgcttcct cgccaacgcc aaaccagaag ttgtccaaaa agaaaaagac aaacaagccg
     7141 actaccaagc taaatacgac gcaacccaag aacggattgt agagatgcaa aaacttgtta
     7201 aatagttaaa atcatccatt acttccgcag agtcctgccg ctagaccatc agtctaagcg
     7261 acagggcttt ttgctatatt caaagattgt gaaaaattaa tatatatgat gcctacttct
     7321 tgcgtctgaa atacaagaag tgaaaatgat ttggccataa agaaaaacag gcagaaaccc
     7381 aaatgattaa tgcctatttc ttatattttt tcgtcaatta ttttataaat aaaattgagg
     7441 tacaaaccag ttttta
//
LOCUS       DATROQ010000088         7164 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.089, whole genome shotgun sequence.
ACCESSION   DATROQ010000088 DATROQ010000000
VERSION     DATROQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.089"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..544)
                     /locus_tag="VBC94_001698"
     CDS             complement(<1..544)
                     /locus_tag="VBC94_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6979954.1"
                     /translation="MLDILSEKHLERRQRIIISAIAIALAAQINISILADGFIMTLSL
                     FILPVFLYFNDDINPILLCLGITFASPIFRGIILSIAGEAEIHQIIEFVLTDMAFYIC
                     YGITFYTIYWHRSYRNKGTFFFSIIICDYFANLVEISFLIKFNNYTITIFATLFAIAL
                     LRAFISCAVAYTYSYLSLLLQ"
     gene            647..1645
                     /gene="argF"
                     /locus_tag="VBC94_001699"
     CDS             647..1645
                     /gene="argF"
                     /locus_tag="VBC94_001699"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO6979955.1"
                     /translation="MKNLRNRSFLTLLDFSTAEVEFLLKLSEDLKRAKYAGIEQQKLV
                     GKNIALIFEKDSTRTRCAFEVAAHDQGAHVTYLGPTGSQMGKKETSKDTARVLGGMYD
                     GIEYRGFSQETVETLAEFSGVPVWNGLTDADHPTQVLADFLTAKECLHKPYKDIRFTY
                     VGDGRNNVANALMIGASIVGMTYHLVCPKELEPDPELLSKCQEIAKTTGASIEITADI
                     AEGVRDSDVLYTDVWVSMGEPDEVWKERIALLEPYRITQEMLNMTENPNVIFEHCLPS
                     FHNIDTKVGYDIYEKYGLKEMEVSDEVFEGPHSVVFQEAENRMHTIKAVMVATLGD"
     gene            1657..2583
                     /gene="arcC"
                     /locus_tag="VBC94_001700"
     CDS             1657..2583
                     /gene="arcC"
                     /locus_tag="VBC94_001700"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO6979956.1"
                     /translation="MAKIVVALGGNALGNSPEEQLRLVKHTAKSLVALIKKGHEIVVS
                     HGNGPQVGAINLGMNFAAESGQGTNFPFPECGAMSQGYIGYHLQQSLLNELRQEGINK
                     EVATIITQIEVDESDQAFSAPTKPIGTFYDKETSEKIAIEKGYTFVEDAGRGYRRVVA
                     SPEPKKIIEINSIKTLIENDTLVIAGGGGGIPVINKGGYEGIAAVIDKDKSSALLAGE
                     LAADQLIILTAVDYVYTQFGKENQKALTEVNENQMIDYVNQGEFAKGSMLPKVIACMS
                     FLDHNPKGIALITSLNGLEDALDGKLGTRITK"
     gene            2677..4230
                     /locus_tag="VBC94_001701"
     CDS             2677..4230
                     /locus_tag="VBC94_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEO6979957.1"
                     /translation="MPEKMTNSENKRSKGFRMPGAFTILFILTIFSVLATWWIPAGSY
                     SKLQFDTASSKLVVTDPNGKTVHVPATQTQLDKMNVKIKIKEFTSGAISKPVSVPNTY
                     KRLKQNPAGIGSVTTSMVNGTIEAVDIMVFIMVLGGMIGVVRKSGAFESGLLALTKKT
                     KGREFLLIFLVSLLMVLGGTLCGIEEEAVAFYPILVPIFLAMGYDSIICVGAIFLASS
                     VGTSFSTINPFSSVIASNAAGISFTEGLSWRTAGCIAGAIFVVVYLHWYAKKIKANPE
                     FSYSYEDRVEFNAKWGMTTNHTPSLFTIRQKIILSLFVISFPLMVWGVMSQGWWFPTM
                     ASSFLAITIIIMFLTATGANGIGERDVVDEFVNGASSLVGVSLIIGLARGINIILSQG
                     YISDTMLYTASKLASHVSGPVFIIVMMFIYFVLGFVVPSSSGLAVLSMPILAPLADTV
                     GIPRSVVVMAYQFGQYAMLFLAPTGLVMATLQMLDMKYSHWLKFVWPVVLFLLIFGGG
                     LLVLQVLIL"
     gene            complement(4340..5704)
                     /locus_tag="VBC94_001702"
     CDS             complement(4340..5704)
                     /locus_tag="VBC94_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6979958.1"
                     /translation="MLFQSKINYPKKSSIITQITLWYSSFIFILVIGVLIGSFFISKS
                     IAENKSKKNLEAKAVQMSQALAKGHRYEAFEDGIFYSVYDQNGKVIYSGFPKGFKRDL
                     DHQHKHKKKLSLFSMENRTFQYVDIPISGKNQWLRAIRTVDRLDKQLTELLFSLGIVL
                     PLMLIIITVGGYLILKRAFRPIQEITETAQFITQNEDYTKRIITKNNENELTELAAVI
                     NTMLASIESSFVREKQFNNDVSHELRTPVTVILSESEYGKNYAENLSEAKESFEVIHR
                     QSLSMKKLVEQLLELTKAENPLSIQLEPLNFSIMMKQLVSDSSRLLDNTPIHLDSQIE
                     DDLWIIGQQTLLKRLFDNLFSNAIKFTNNHISISLRQSDNQIVFSIKDNGLGISVDDQ
                     SKIWNRFYQVDSARTKDSQSGIGLGLSLVKQIATIHRAKIWVDSKPDDGSQFTLTFPK
                     LKKD"
     gene            complement(5688..6359)
                     /locus_tag="VBC94_001703"
     CDS             complement(5688..6359)
                     /locus_tag="VBC94_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6979959.1"
                     /translation="MKILVVEDEFDLNRSIVKLLKKQHYSVDSASNGEEALQFVSVAE
                     YDVIILDVMMPKMDGFTFLKLLRNKGSQVSILMLTARDAVEDRIAGLDFGADDYLVKP
                     FEFGELMARIRAMLRRTNRQVSSDDIQIQDITINLSTKQVWRNDNLIDLTAKEYEVLE
                     YLARHRDQVLSRHQIREHVWDYDYYGESNIIDVLIKNLRRKLDNNRDGSLIKTKRGLG
                     YVIPK"
     gene            complement(6444..7115)
                     /locus_tag="VBC94_001704"
     CDS             complement(6444..7115)
                     /locus_tag="VBC94_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO6979960.1"
                     /translation="MTNYKNNFKKFMSNKLYVGLASITLLGIGGAIGVSGAALAHNHH
                     SEHMSFEHKHEGHDKHHDHREKYSLKTLKNISTKIDYKTALETAKKEARSGFISQIEL
                     NTKGDTPFYEIKVIDNKIAKKYKIDANSGKILSTTSDNQLNSKDAQLKQPSKDIQTAL
                     QAIQAKYKNAKIKEISLANNKSNKLVYKVKLLDGNTPKIAQFDSDKASFIKANKLNTT
                     HKNHD"
BASE COUNT         2254 a         1125 c         1354 g         2431 t
ORIGIN      
        1 tttgtaagag aagtgataga taactataag tataagccac agcgcaacta ataaaagctc
       61 gcaaaagtgc tatggcaaat aaagtagcga atatagttat agtataattg ttaaacttta
      121 tgagaaaact aatttctaca aggttggcaa aataatcaca aataatgata gaaaagaaaa
      181 aagtaccttt attacggtag gaacgatgcc aataaatagt ataaaaggtg atgccataac
      241 aaatataaaa cgccatatca gttaaaacga attcaattat ttgatgaatc tcagcctcgc
      301 cagcgatact taatatgatt cctctgaaga taggagaagc aaaagtaatt cctagacaga
      361 gaagaattgg attaatgtcg tcattaaaat ataaaaagac aggcaaaatg aacaaagata
      421 aggtcataat aaacccatca gcaagtattg agatattgat ttgagcagct aaggcgatag
      481 ctattgcaga gataataata cgctgccttc tttctagatg tttttctgat aatatatcta
      541 gcatataaat gtccttaaag tttttttgaa gatttttgat gaattttgat tatttttgaa
      601 agcgctatct acaataagag tataaaaaaa aaggaggcaa taagcaatga aaaatttacg
      661 taaccgtagt tttcttactt tattggattt tagtacagca gaagttgaat ttcttttaaa
      721 actttcagaa gatttaaaac gtgctaaata cgcaggaatt gaacaacaaa aacttgttgg
      781 taaaaatatc gctttgattt ttgaaaaaga ttctacacgg acacgttgcg cttttgaagt
      841 tgctgctcac gatcaaggag ctcatgttac ttatttaggt ccaacaggca gtcagatggg
      901 taaaaaagag acctcaaaag acactgcgcg tgtgctaggt ggtatgtatg atggtattga
      961 gtatcgtgga ttttctcaag aaacagttga gactttagcc gaattttcag gagttcctgt
     1021 gtggaatggt ttaaccgatg ctgatcatcc aacgcaagta ctagccgatt ttctgaccgc
     1081 taaggaatgc ttacataagc cctataaaga tattcgcttt acgtatgtgg gtgatggacg
     1141 taacaatgtc gctaatgcct tgatgatcgg tgcttctatt gtaggcatga cttatcattt
     1201 ggtttgtcct aaagaattag agccagatcc tgaactactc tctaaatgtc aagaaattgc
     1261 gaaaactact ggtgcttcta ttgaaatcac tgctgatatt gcagaaggcg tcagagattc
     1321 agacgtccta tatacagatg tttgggtttc aatgggagaa ccggatgaag tttggaaaga
     1381 aagaatagct cttttagaac cctatcgtat tacccaagaa atgcttaata tgacagaaaa
     1441 tccaaacgtt attttcgagc attgcttgcc ctcatttcat aatatagata ctaaagttgg
     1501 atacgacatt tatgaaaaat atggtttgaa ggaaatggaa gtatcagatg aagtttttga
     1561 aggccctcac tcagttgttt tccaagaagc tgaaaatcgt atgcatacta ttaaagcagt
     1621 tatggtagca actttaggtg attaggagga attttcatgg caaaaattgt tgttgcacta
     1681 ggaggaaatg ccttaggaaa ttctccagaa gaacaattaa gattagtaaa acatactgca
     1741 aaatcattgg tggccttgat aaaaaaggga cacgaaattg ttgtaagtca tggtaatgga
     1801 ccacaagttg gtgccattaa tttaggaatg aattttgcgg ctgagagtgg tcaaggaact
     1861 aattttcctt ttccagaatg tggagcaatg agccaaggat acataggcta tcatctccaa
     1921 cagagccttt taaatgaatt acgtcaagag ggaataaata aggaagttgc tacaattatt
     1981 acccaaattg aggtggatga atcagaccaa gcatttagcg cacctaccaa gcctataggc
     2041 accttttatg ataaggagac atcagaaaag attgctatag agaaagggta tactttcgta
     2101 gaagacgctg gacgagggta tcgtcgtgtt gttgcttcac ctgaacctaa aaaaatcatc
     2161 gaaattaata gcattaaaac acttattgaa aatgataccc tagttatcgc aggtggaggt
     2221 ggaggcatcc ctgtcattaa taagggaggt tatgaaggta ttgcagctgt tattgataaa
     2281 gacaaatcta gtgcactgtt agcaggagaa ctagctgcgg atcaattgat tattctcact
     2341 gcagttgatt atgtttatac tcaatttggt aaagaaaatc aaaaagcttt aacagaggtt
     2401 aatgagaatc aaatgattga ttacgttaac caaggtgagt ttgcaaaagg aagtatgtta
     2461 cctaaagtaa tagcctgtat gagtttctta gatcacaatc cgaaaggaat agccttaatc
     2521 acctcattaa atggtttaga agatgcatta gatgggaaat taggaacacg tataactaag
     2581 taatctcaga taagtgcaaa gaaatgatta tttaaccttc atctttgcac tttttctgat
     2641 gaagtcaatc tatttttatt atagaggagg cgtgtcatgc cagaaaaaat gacaaacagt
     2701 gaaaacaaaa ggagtaaagg ttttcgaatg ccaggtgcct ttacaatatt attcatttta
     2761 acaatctttt cggttttggc tacatggtgg atacctgctg ggtcctattc aaaattacaa
     2821 tttgatacag catcatccaa actagtagta acagatccaa atggtaagac agttcatgtt
     2881 ccagcaacgc aaacacagtt ggataagatg aatgtcaaaa ttaaaattaa agaatttact
     2941 tctggtgcta ttagtaagcc agtttcagtt ccaaatactt acaaacgttt gaagcaaaat
     3001 ccagcaggca ttggctccgt gacgactagt atggtaaatg gaaccattga agctgttgat
     3061 attatggtat ttatcatggt acttggaggc atgataggag tagttagaaa gagtggcgct
     3121 tttgagtctg gtcttctagc tttaacaaag aaaaccaaag gtagagagtt tttattgata
     3181 tttttagttt ccttattgat ggttttaggc ggtactctat gtggtattga agaagaagca
     3241 gttgcttttt atcctatttt agttccaatc tttttagcca tgggttatga ttctatcatt
     3301 tgtgtaggtg ctattttctt agcaagttct gttgggacat cattttcaac aattaatccg
     3361 ttttcttctg ttattgcttc aaatgcagct ggtattagtt tcacagaagg cttatcatgg
     3421 cgtacagcag ggtgtattgc aggtgctatt tttgttgtgg tgtatctaca ttggtatgct
     3481 aaaaaaataa aagcgaatcc agagttttca tattcctatg aagatagagt agaatttaat
     3541 gctaaatggg gcatgacaac taaccataca ccctcactct ttactattag acaaaaaatt
     3601 attttaagtt tatttgttat ttcatttcct ttgatggttt ggggagtaat gtcacaggga
     3661 tggtggtttc caaccatggc ttcttccttt ttagctatta caattattat tatgttttta
     3721 acagcaactg gggcaaatgg tattggagaa agggatgttg ttgatgagtt tgttaatggt
     3781 gcatcaagcc ttgttggggt ttcattaatc attggtttag cgcgtggtat caatattatt
     3841 ttgagccaag gttatatatc agatacgatg ctttacacag cttcaaaatt agcttcacat
     3901 gttagtggcc ctgtctttat cattgttatg atgtttatct actttgtgtt aggatttgtt
     3961 gttccatcct cgtcgggttt agcagtactt tcaatgccaa ttctagcacc tcttgcagat
     4021 actgttggga ttcctcgttc agtagtagta atggcttatc aatttgggca gtatgcaatg
     4081 ttgtttttag cacctactgg actagttatg gcaaccttgc agatgttaga tatgaagtac
     4141 tcccattggc taaaatttgt gtggccggtt gttttattcc tcctaatatt tggtggcggt
     4201 ttgttagttc tacaagtttt aatactttaa agatttcagc tattagtctc aaaaatatct
     4261 gaagagatat aataaattga gtttaagtta aataaaaaaa acgataggtg taatctatcg
     4321 ttttttttat ttcctttctc tagtctttct tcaattttgg aaaggtgagc gtaaactgac
     4381 tgccatcatc aggtttagaa tcaacccata ttttagcacg gtgtattgta gcgatttgtt
     4441 ttactaaaga tagccctaaa ccaattccag actgactatc cttggttcga gcagagtcca
     4501 cttgatagaa acggttccaa attttacttt ggtcatcaac tgagatacct agaccattat
     4561 ctttaataga gaagacaatt tgattatcgg actgtcttag actaatagaa atatgattgt
     4621 ttgtaaattt aatagcattt gaaaatagat tatcaaaaag ccgtttgagt agtgtttgtt
     4681 gacctataat ccaaaggtca tcttcgattt gagagtcaag gtgtataggg gtattatcta
     4741 ataatctact cgaatcggat actagttgct tcatcataat tgagaaatta agaggttcta
     4801 actgaattga taatggattt tctgccttag tcaattctag aagttgctca actaattttt
     4861 tcattgatag tgactgacgg tgtataacct caaaggattc ttttgcttca cttaaatttt
     4921 ctgcgtagtt ttttccgtat tcactttcag aaagtataac tgttacagga gtccgtagtt
     4981 catgagaaac atcattgtta aattgttttt cccgaacaaa tgatgattca atagaagcca
     5041 acatagtatt gataacagct gctagctcag ttaattcatt ttcgttattt ttagtgataa
     5101 ttcgcttagt atagtcttca ttttgggtaa taaattgagc ggtttcagta atttcttgaa
     5161 tcggtctaaa agcgcgcttt aaaattaaat agccaccaac agttattata atcaacataa
     5221 gaggaagaac gatacccaaa ctaaataaca attcagttaa ttgcttatcc agtctgtcaa
     5281 cagtacggat agcacgtaac cactgatttt tcccagaaat tggaatatca acatactgaa
     5341 aggtacgatt ctccatggaa aatagagata atttcttttt atgtttatgt tgatggtcta
     5401 aatccctttt aaatcctttt ggaaaaccag aatagataac ttttccattt tggtcataga
     5461 ctgaataaaa tattccatct tcaaaagcct cataccgatg acctttagct aacgcttgac
     5521 tcatttgcac agctttagct tctaaatttt ttttagattt gttttcagca atgctttttg
     5581 aaataaaaaa tgaaccaatt aatacaccga taacaaggat aaaaataaaa gagctgtacc
     5641 agagggtaat ttgagtgatg atgctagact ttttagggta atttatttta ctttggaata
     5701 acatatccta aacctcgttt agtttttatt agtgatccat ctcgattgtt atctagtttt
     5761 cgacgaaggt ttttgataag gacatcaata atattggact ctccataata atcataatcc
     5821 caaacgtgtt cacgaatttg atgacgagag aggacttggt ctctgtgacg tgccaaatac
     5881 tcaaggactt cgtattcctt agctgttaaa tcaatcaaat tgtcgtttct ccaaacttgc
     5941 tttgtagata aattgattgt tatatcttga atttgaatat catcagaaga tacttgtcta
     6001 tttgtacgtc gtaacatagc tcgaattcgg gccatgagtt ctccaaattc aaatggtttt
     6061 acaaggtaat catccgcacc aaaatctaga cctgcgatac gatcttcaac ggcatctcga
     6121 gcagtcaaca taagaataga tacttgactt cctttgttgc gaagcagttt taaaaaggtg
     6181 aagccgtcca tttttggcat cattacatct aagataatca catcatattc tgccactgaa
     6241 acaaattgta aggcttcttc accatttgaa gcactgtcaa cactgtagtg ttgctttttt
     6301 aaaagtttta caatactacg gttcaagtca aactcatctt caacaacaag gattttcatt
     6361 ttttggcctc cgtatggctt ttttccatta tatcacaaaa gggaatggag tactgaggtc
     6421 ctattccctt taatacacta gtattagtcg tgatttttat gagttgtatt aagcttattt
     6481 gcctttataa atgatgcttt atcactatca aattgggcaa tcttaggagt atttccatca
     6541 agtagtttta ctttataaac tagcttattt gatttgttat tcgcaagaga aatttctttt
     6601 atttttgcat ttttatattt tgcttgaata gcttgtaatg cagtctgaat atctttactt
     6661 ggttgtttta attgggcatc ttttgaattg agttgattat ctgatgttgt tgaaaggatc
     6721 ttcccagaat tagcatcaat tttatatttt ttagctattt tattatcaat aactttgatt
     6781 tcataaaaag gtgtatcacc tttggtattt aattcaattt gtgaaataaa gcctgagcga
     6841 gcttcttttt tggcagtttc taaggcagtc ttataatcga tttttgtcga gatattcttt
     6901 aatgttttta gtgagtattt ttctctatga tcgtgatgtt tatcgtgtcc ttcatgctta
     6961 tgttcaaaag acatgtgttc tgagtgatgg ttatgcgcta atgcagctcc acttactcct
     7021 atagctcccc caataccaag tagcgtaatg gaagctaacc ctacgtataa tttattgctc
     7081 atgaattttt taaagttgtt tttatagttt gtcatactga cttccttctt tcttttttca
     7141 aaccttggat taggtttgtt actt
//
LOCUS       DATROQ010000089         7106 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.090, whole genome shotgun sequence.
ACCESSION   DATROQ010000089 DATROQ010000000
VERSION     DATROQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.090"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            61..1344
                     /gene="aroA"
                     /locus_tag="VBC94_001705"
     CDS             61..1344
                     /gene="aroA"
                     /locus_tag="VBC94_001705"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEO6979961.1"
                     /translation="MKLLTNANTLKGTIRVPGDKSISHRAIIFGSISQGVTRIVDVLR
                     GEDVLSTIEAFKQMGVLIEDDGEIITIYGKGFAGLTQPNNLLDMGNSGTSMRLIAGVL
                     AGQEFEVTMVGDNSLSKRPMDRIALPLSKMGARISGVTNRDLPPLKLQGTKKLKPIFY
                     HLPVASAQVKSALIFAALQTKGESLIVEKEQTRNHTEDMIRQFGGHLDIKDKEIRLNG
                     GQSLVGQDIRVPGDISSAAFWIVAGLIIPNSHIILENVGINETRTGILDVVSKMGGKI
                     KLSSVDNQVKSATLTVDYSHLQATHISGAMIPRLIDELPIIALLATQAQGTTVIADAQ
                     ELKVKETDRIQVVVESLKQMGADITATADGMIIRGNTPLHAASLDCHGDHRIGMMIAI
                     AALLVKEGEVDLSGEEAINTSYPNFLEHLEGLVNA"
     gene            1337..1849
                     /locus_tag="VBC94_001706"
     CDS             1337..1849
                     /locus_tag="VBC94_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEO6979962.1"
                     /translation="MPKVLLGFMGVGKTSVANCLENEVIDMDSLIEKHIGMSISRFFT
                     EEGEASFRALESQFLNELLKKKNEGLVIASGGGIVLLEENRRLLTLNRHNNILLTGSF
                     EVLYHRIKKDEKNRRPLFLNHSKEEFYDIYQKRMLLYSGLSDMIIDTDYLTPQKIATV
                     IGESLVNLSN"
     gene            1906..3279
                     /locus_tag="VBC94_001707"
     CDS             1906..3279
                     /locus_tag="VBC94_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6979963.1"
                     /translation="MSRNNYGQLNHHEELRYNYLLKNIHYLNEREKMEFQYLHYKKTA
                     VRPQRRTESPPTNSYYEEPYSDSYYQDDDFYSEPQLTSQGLPIYQEERAPKKKKQRAR
                     KEKQRVKVMAPFPPKAITPPRKKKKFKGFLKFIGIILLIVLSGMVFMFVKGMRDVNNG
                     KSHYSPAIIEDFKGKDAVDGTNILILGSDKRVSERSTDARTDTIMVANVGNKDNKVKM
                     VSFMRDLLVNIPNYSTEGYYDMKLNASFNLGEQDNHKGAEYVRQTLKNHFDIDIKYYV
                     MVDFETFADAIDTLFPNGVKINAKFGLVGGQSADSVKVPDDLRMKNGVVPSQKIKVGI
                     QYMDGRTLLNYARFRKDDDGDFGRTQRQQQVMRAIVSQIKDPRRLFTGSAAIGKAYAL
                     TSSNLSYSFVLTDGIPILSDAKNGIKQMTIPREGDWVDDYDQYGGQGLTIDFAKYKKI
                     LKKMGLR"
     gene            3380..4735
                     /gene="rlmD"
                     /locus_tag="VBC94_001708"
     CDS             3380..4735
                     /gene="rlmD"
                     /locus_tag="VBC94_001708"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO6979964.1"
                     /translation="MLHKNDIIETEISDISHEGMGIAKVDGFVFFVENALPGEIIKMR
                     VLKLRKRIGYGKVEEYLTTSPHRNEGLDYTYLRTGIADLGHLTYEQQLLFKQKQVADN
                     LYKIAHISDVLVEPTLGMTIPLAYRNKAQVPVRRVDGQLETGFFRKNSHTLVSIEDYL
                     IQEKEIDALINFTRDLLRKFDVKPYDEEQQSGLIRNLVVRRGHYTGQLMLVLVTTRPK
                     IFRIDQMIEKLVSAFPSVVSIMQNINDRNSNVIFGKEFRTLYGSDTIEDQMLGNTYAI
                     SAQSFYQVNTEMAEKLYQKAIDFSDLNSEDIVIDAYSGIGTIGLSVAKQVKHVYGVEV
                     VEKAVSDAKENATRNGITNSTYVADSAENAMAKWLKEGIKPTVIMVDPPRKGLTESFV
                     YSAAQTKADKITYISCNSATMARDIKLFEELGYHLVKIQPVDLFPMTHHVECVALLVK
                     A"
     gene            4843..5064
                     /locus_tag="VBC94_001709"
     CDS             4843..5064
                     /locus_tag="VBC94_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979965.1"
                     /translation="MPTMQLLDELVLPKDITIADVSQSIQEFEIERDALQEFFDDMLK
                     TYTTYQEMKDYIKSREANTPKQETEEKSL"
     gene            5182..5919
                     /gene="aphA"
                     /locus_tag="VBC94_001710"
     CDS             5182..5919
                     /gene="aphA"
                     /locus_tag="VBC94_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEO6979966.1"
                     /translation="MKKVLVSSLLVLGITITLQTVVEAKGPKVAYTQEGMTALSDTNK
                     DKVTTISIDEIQKSLEGKKPITVSFDIDDTLLFSSQYFQYGKEYVTPGSFDFLHKQKF
                     WDLVAKRGDQDSIPKEYAKKLIAMHQKRGDKIVFITGRTRGSMYKEGEVDKTAKALAK
                     DFKLDKPIAVNYTGDKPKKPYKYDKSYYIKKYGSDIHYGDSDDDIHAAREAGARPIRI
                     LRAPNSTNLPLPEAGGYGEEVLENSAY"
     gene            6240..6758
                     /locus_tag="VBC94_001711"
     CDS             6240..6758
                     /locus_tag="VBC94_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEO6979967.1"
                     /translation="MRVYENKEELKKEISKTFEKYIMEFNNIPENLKDKRIDEVDRTP
                     AENLSYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSIYAMIDLLSEEELFEAHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(6886..7074)
                     /locus_tag="VBC94_001712"
     CDS             complement(6886..7074)
                     /locus_tag="VBC94_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001875973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEO6979968.1"
                     /translation="MITHSDIPDLQKQLETAYQVPYSFALGIYISTIESIILNWIDHD
                     FTEEPEEIARYITSVVRI"
BASE COUNT         2502 a         1024 c         1378 g         2202 t
ORIGIN      
        1 aattcaaaat atagcatata ttatgataca ataatagcaa aatagatttt gggagttatc
       61 atgaaattac tgactaatgc aaatactctc aagggaacca tccgtgttcc tggcgataaa
      121 tcaataagtc acagggctat tatttttggg agtatatctc agggagttac ccgaattgtt
      181 gatgtcttaa gaggcgagga tgttttatca actatagagg cttttaaaca aatgggggtt
      241 ctaattgaag acgatggcga gataatcact atctatggca aaggatttgc aggtttaaca
      301 caaccgaata atctcttaga catgggaaac tctggtactt caatgcgtct gatagcaggt
      361 gtgttagctg gtcaagagtt tgaagtgacg atggttggag acaatagttt gtcaaagaga
      421 cctatggatc gtatagcatt gcctttaagc aaaatgggag ctagaatttc gggagtaact
      481 aaccgtgatt taccaccatt aaaacttcaa ggtacaaaaa aattaaaacc tattttttac
      541 catttacccg tagcttctgc acaagtaaaa tcagctttaa tttttgcggc tttacaaact
      601 aagggagaat ctctcattgt agaaaaagag caaacaagga atcatactga ggacatgatt
      661 cggcaatttg gtggacattt ggatattaaa gataaagaaa ttcgactcaa tggcggtcag
      721 agcttagtag gacaagatat aagagttcct ggtgatattt cgagtgctgc cttttggata
      781 gtagcgggtc ttattattcc taacagtcat attattttag aaaacgtagg tataaacgag
      841 acacgaactg gaatccttga tgtcgtcagt aaaatgggtg gaaaaattaa gttatcttcg
      901 gttgataatc aagtaaaatc agcaaccctt acagttgatt actctcatct acaagctact
      961 catatttctg gggctatgat tccacgtctg attgatgaat tacctattat tgctctttta
     1021 gcaacacaag ctcaaggcac cacagtcata gcagatgctc aggaattaaa ggtgaaagag
     1081 acagatcgga ttcaggtcgt tgtcgaatcg ctaaaacaga tgggtgctga tattactgct
     1141 acagcagatg gtatgattat acgtggtaat acccctcttc acgctgcttc acttgattgt
     1201 catggagatc atagaatagg aatgatgata gctattgctg ctctcttggt caaagaaggc
     1261 gaagtagatt tatctggtga agaagctatt aatacaagct atcctaactt tttagaacat
     1321 ttggagggat tagttaatgc ctaaggtatt attgggattc atgggagttg gcaagactag
     1381 tgttgccaac tgcttagaaa atgaagtaat tgatatggat agtctgattg aaaaacatat
     1441 tggtatgtca atttcccgat tttttacaga agaaggagaa gcctcctttc gtgctttaga
     1501 atctcaattt ttaaacgaac ttttaaagaa aaaaaatgaa ggacttgtga tagctagtgg
     1561 aggaggtatt gtactccttg aagaaaatcg tcgtctttta actttaaatc gtcataataa
     1621 tattctttta acaggaagtt ttgaagtctt atatcatcgt ataaaaaaag atgagaagaa
     1681 tagaagacca ttatttttaa accattcaaa agaagaattt tatgatattt atcaaaaacg
     1741 catgttactc tatagtggtt tgtctgatat gattattgat accgattatc ttacaccaca
     1801 aaaaatagct actgtaattg gagagtcact ggttaattta tctaactaag ttatcacagt
     1861 ttggtagtat gaactattat tattgaaagt aaatcacctg aaaatatgtc aagaaataat
     1921 tacggtcagc taaatcatca tgaagagcta cgatataact atcttttaaa aaacatccat
     1981 tatcttaatg aacgtgaaaa gatggaattt cagtaccttc attataaaaa aacagcagta
     2041 cgaccacaga gaaggacaga aagtccacca actaattctt attatgagga accttattct
     2101 gattcttatt atcaagatga cgatttttac tcagagcctc agctaacctc acaaggttta
     2161 ccaatttatc aagaagagag agctccgaag aagaaaaaac aaagggccag aaaagaaaaa
     2221 cagagggtga aggttatggc gcctttccct ccaaaggcta taaccccccc tagaaagaaa
     2281 aaaaaattta agggtttcct aaagttcata ggtattatat tactaattgt tttatcaggt
     2341 atggttttca tgtttgttaa aggaatgcgt gatgtgaata atgggaagtc acattatagc
     2401 cctgcaatta ttgaagattt taagggaaaa gatgctgttg atgggactaa tatcttaatt
     2461 ttaggtagtg ataaacgtgt cagcgagaga tctactgatg cgagaacaga taccataatg
     2521 gttgctaatg ttggtaacaa ggataataag gttaagatgg tgagttttat gcgagatctc
     2581 ttagtcaata ttcctaatta tagtacggaa ggttactatg atatgaaatt aaatgcgtct
     2641 tttaatttgg gagaacaaga taatcataaa ggagcagagt atgttcgaca aacgttgaaa
     2701 aatcattttg atattgatat aaaatattat gttatggttg actttgagac atttgcagat
     2761 gctattgata cactgtttcc taatggtgtc aagattaatg ctaaatttgg acttgtcggt
     2821 ggacaatctg ctgattcagt taaagttcct gatgatttaa ggatgaaaaa tggcgttgta
     2881 cctagccaaa aaattaaggt tgggattcag tacatggatg gaaggaccct tctaaattat
     2941 gctcgtttca gaaaagacga tgatggggac tttggtcgaa cacaacgtca acaacaagtt
     3001 atgagagcta ttgtctctca aataaaagac ccacgtcgtc tctttacagg ttctgctgct
     3061 atcggtaaag cctatgcttt aacttcaagt aatttatctt atagctttgt cctaacagat
     3121 ggcattccaa ttttatcaga tgctaaaaat ggtattaagc aaatgacaat tccaagagaa
     3181 ggagattggg tagatgatta tgaccagtat ggtggtcaag ggttaacaat tgattttgct
     3241 aagtacaaaa agatcctaaa gaaaatggga ctgcgttaac atttttaatt atgatataat
     3301 agagtgattt attgtcactc tatttttatc tttagtgtat aaactaaaga cttcaaatga
     3361 tagatataaa aggaaaatta tgttacataa aaatgatatt attgaaactg aaattagcga
     3421 cattagccat gagggaatgg gtattgccaa agtagatggc tttgttttct ttgttgaaaa
     3481 tgctcttcca ggtgagatta ttaagatgcg cgtcttgaaa ttacgtaagc gtataggata
     3541 tggaaaagtt gaggaatatt taacgacttc tcctcaccga aatgaaggat tagactatac
     3601 ctatttacgt acagggattg ctgatttggg acatcttact tatgaacaac agctgttatt
     3661 taaacaaaaa caagtagctg ataaccttta taaaattgct catatatctg atgttttagt
     3721 agaacctact cttggtatga caatcccttt ggcatatcga aataaagctc aggtaccagt
     3781 tcgtcgtgtt gatggacagc tagagacagg ttttttcaga aaaaattcac atactctggt
     3841 atccattgaa gattatttaa ttcaggaaaa agagattgat gctctgatta attttacacg
     3901 agatttatta agaaaatttg atgttaaacc atatgacgaa gagcagcaat ctggtttaat
     3961 tagaaatctc gttgttcgtc gaggacatta tacagggcaa ttaatgttgg ttttggtgac
     4021 aacacgtcct aaaatattcc gcattgacca aatgattgaa aagttggtgt ctgcctttcc
     4081 ttctgttgtt tcaattatgc aaaatattaa tgatcgtaat agcaacgtta tttttgggaa
     4141 agaatttcgt actctatatg gttctgatac tattgaggac caaatgttag gaaatactta
     4201 cgctatatca gcccaatctt tttaccaagt taatactgaa atggctgaaa aactgtacca
     4261 aaaagcaatc gatttttcag atttaaactc agaggatatc gttattgatg cctattcagg
     4321 tataggaaca attggcttat ctgttgctaa acaagtaaaa catgtttatg gtgttgaagt
     4381 tgttgaaaaa gcagtaagtg atgccaaaga aaatgctact cgaaatggga taactaacag
     4441 tacctatgtt gctgattcgg ctgaaaatgc aatggcaaag tggcttaaag aaggtataaa
     4501 accaactgtt ataatggttg atccaccacg aaaaggatta acggagagtt ttgtatattc
     4561 tgctgcccaa acaaaagcag ataaaatcac ttatatttct tgtaattcag cgacaatggc
     4621 gcgtgatatc aaactatttg aagaattagg gtatcattta gtaaaaattc aaccagtaga
     4681 cttattccca atgacacatc atgtggagtg tgtggctctg ctcgtaaaag cctagaaact
     4741 ttttaacgaa aggaatatta aaaagcctta gataatcgac tgacctatta ccaaaaaatc
     4801 gaaggggctc tacttgccat tgcacaaaca ccaggaaata gtttgccaac tatgcagctg
     4861 ttagatgagt tagttcttcc aaaagacatt accatagcag atgtcagtca aagtatccaa
     4921 gaatttgaaa tagaaagaga tgcccttcaa gaattttttg acgatatgct gaagacttat
     4981 acaacctacc aagaaatgaa agattatatt aaatctcgtg aagcgaacac tcctaagcaa
     5041 gagacagaag aaaaatcact ttgaagactg tagacttaac ataagtaaaa aataaccata
     5101 tatgatatcg ctttcattct tatgtttttt gtgataaact aattaaagaa ataattttta
     5161 tactttatta tggaggttct tatgaagaaa gtgttagtga gtagtctttt ggttttaggg
     5221 attacgataa cgttacaaac agtagttgag gctaaggggc caaaagtagc ttatacacaa
     5281 gagggaatga ctgctctttc ggacacaaat aaagataaag tcactactat ttctattgac
     5341 gagattcaaa aaagcttaga aggtaagaag ccgattactg ttagttttga tattgatgat
     5401 acactgcttt tcagtagtca atattttcaa tatggtaaag aatatgtaac tcctggatcg
     5461 tttgattttc ttcataaaca aaaattctgg gatcttgttg caaaacgagg agatcaagat
     5521 tccattccca aagaatatgc taaaaaatta attgctatgc atcaaaaacg aggagataaa
     5581 attgttttta taacaggtag gacaagaggg tcaatgtata aggagggcga ggttgataaa
     5641 acagctaaag ccttagctaa agattttaaa ttagacaaac caattgctgt aaattataca
     5701 ggcgataaac ctaaaaagcc atacaaatat gataaatcat attatattaa gaaatatggt
     5761 tcagacattc attatggaga tagtgatgac gatattcatg cagctaggga ggccggtgct
     5821 agaccaatta gaattttaag agcacctaat tctacaaatc tacctttacc agaagctgga
     5881 ggctacggtg aagaggttct cgaaaattca gcttactagt atctttatta gttaacatag
     5941 tattatctat caatttttag atttgtaata agaagtgcta aaaaagtagc aaatcaatga
     6001 tatgctaata agggaagttg gagtagttta gattgattat taatgactga tcttctctcc
     6061 tctcggtaac aagaagtttt tctaacaaat gcctgtctaa aaaaattgaa tcataatttt
     6121 ttaataactc ttttggcgtg aacaaggaat ttgtaatcag tcatctaatt ctataataca
     6181 atactttggg gttgtttccg tatcattatt aataatatgt atcagaaagg agatgttttt
     6241 tgagagttta tgaaaataaa gaagagttga aaaaagaaat aagtaaaaca tttgagaaat
     6301 acattatgga atttaataat attccagaga atctaaaaga taaaagaatt gatgaagttg
     6361 atagaactcc agcagaaaac ctttcttatc aggttggctg gaccaacttg gttcttaaat
     6421 gggaagaaga tgaaagaaag ggacttcaag taaaaacacc atcggataaa tttaaatgga
     6481 atcaacttgg tgaattatat cagtggttca cagataccta cgctcattta tctctgcaag
     6541 agttgaaagc aaaattaaat gaaaatatta attctatcta tgcaatgatt gatttgttga
     6601 gtgaggaaga attatttgaa gcgcatatga gaaagtgggc tgatgaagcg actaaaacag
     6661 cgacttggga agtgtataag tttattcatg taaatacggt tgcacctttt ggaactttca
     6721 gaactaaaat cagaaaatgg aagaagatag tattataaat tatattttta actttaaaaa
     6781 atttcataaa aatggttacc aaaggcgata gaagaaaaaa ctatcgtctt tttctttata
     6841 aagtattaga tgaggaaaag acgatagttt tttctaatac aaatattaaa ttctcacgac
     6901 agatgtaata tatcttgcta tttcttcagg ctcttcagta aagtcatgat ctatccaatt
     6961 taaaataata ctttcaattg ttgagatata aataccaaga gcgaaagaat atggaacttg
     7021 atatgctgtt tccagttgtt tttgtaagtc tggtatatcg gaatgtgtaa tcatctcttt
     7081 gatatagttt ttaattatgt tgtcaa
//
LOCUS       DATROQ010000090         7081 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.091, whole genome shotgun sequence.
ACCESSION   DATROQ010000090 DATROQ010000000
VERSION     DATROQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.091"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..432
                     /locus_tag="VBC94_001713"
     CDS             <1..432
                     /locus_tag="VBC94_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6979969.1"
                     /translation="IIQFRHQDIIILENNKCNLIISNYYYLLTIISNIVLNAVEAIDK
                     QKKGTISVHTEELEDFIKIEISDNGPGIPDKMKHMIFKPGFSTKFDANGDIYRGIGLS
                     HVRILMEEQYQGTITVCPNQPNGTTFTLLFRKNRFKLGDNT"
     gene            429..1262
                     /locus_tag="VBC94_001714"
     CDS             429..1262
                     /locus_tag="VBC94_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEO6979970.1"
                     /translation="MKFYIIDDDPTITMILQDIIEEDFNNTVVRVNNVSSKAYNELLI
                     ADVDIVLIDLLMPILDGVTLVQKIYKQRSDLKFIMISQVKDNDLRQEAYKAGIEFFIN
                     KPINIIEVKSVVKRVTDTIEMQKKLNTIQNLLENTPSYQKPITTSNLTKIRSILSYLG
                     ITSETAYTDILNICELLLKQELNFAQFDFQKELSIDEHQQKIILQRIRRAVKKAMINM
                     AHLYIDDFENELTLQYANALFGFQNIHNEAQLIQGKSMYGGKISLKRFFDELILQSKT
                     F"
     gene            1484..2206
                     /locus_tag="VBC94_001715"
     CDS             1484..2206
                     /locus_tag="VBC94_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6979971.1"
                     /translation="MISFENVSKSYGDHTIIDNISCHIQRGEFFVLVGASGSGKTTIL
                     KMINRLIEPSQGAITLDGENITSLDLRQLRLETGYVLQQIALFPNLTVGENIELIPEM
                     KGWSKGDQKKAASDLLDKVGLPAKDYFNRYPHELSGGEQQRIGILRAIVAKPKVLLMD
                     EPFSALDPISRRQLQDITKQLQSELGITLVFVTNDMKEAMRLADRICVIKEGKIVQLD
                     RPEIIQNNPSDQFVRTLFEEEN"
     gene            2209..3723
                     /locus_tag="VBC94_001716"
     CDS             2209..3723
                     /locus_tag="VBC94_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019319700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEO6979972.1"
                     /translation="MTTLITTFQERFGDWTQSLIEHLQLSLLTLILATLIAIPLGIII
                     SHYKKISHVVLQITGIFQTIPSLALLGLFIPFMGIGTVPAVVALIIYALFPILQNTVT
                     ALMQIDANLIEAATAFGMTRWERLKKFELALSMPVIISGIRTASVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNPSLILIGAISSAVLAIIFSGLIGLLEKARLRTIAVSGILLLA
                     GLGLSYAPKWMPGTNTATITVAGKLGTEPDILINMYKELIEDQTDIKVKLKPNFGKTT
                     FLYQALKSGDIDLYPEFTGTITSSLLKNPPKVSNNPKQVYNLAKNGILKQDKLSLLSP
                     MAYQNTYAVAVKKDYAEANQLKNISDLKKLDKLKAGFTLEFKDREDGSIGLQKHYGLN
                     LDISTLEPALRYQAINSKDVNIIDAYSTDSELIQYQLQILKDDKHLFPPYQGAPLLRQ
                     DTIKKYPQVKKALNKLAGHITEKEMQEMNYQVAVKHKSAATVAKQYLKAHHIIK"
     gene            complement(3802..6342)
                     /gene="mprF"
                     /locus_tag="VBC94_001717"
     CDS             complement(3802..6342)
                     /gene="mprF"
                     /locus_tag="VBC94_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEO6979973.1"
                     /translation="MKKLIEKVKSLTSVIKIVFFISVLVLIIVEMIHLKRTISVEQLK
                     SVFGQLSPMNLFLIILVGVIAVLPTTGYDFVLNGLLRTDKSKRYILQTSWCINTFNNL
                     SGFGGLIDIGLRMAFYGKKGQEKSDLREVTRFLPYLISGLSFISVIALIMSHIFHAKA
                     SVDYYYLVLIGASMYFPVIYWISGHKGSHYFGDMPSSTRIKLGIVSFFEWGCAAAAFI
                     IIGYLMGIHLPVYKILPLFCIGCAVGIVSLIPGGLGSFELVLFTGFAAEGLPKETVVA
                     WLLLYRLAYYIIPFFAGIYFFIHYLGSQINQRYENVPKELVSTVLQTMVSHLMRILGA
                     FLIFSTAFFENITYIMWLQKLGLDPLQEQMLWQFPGLLLGVCFILLARTIDQKVKNAF
                     PIAIIWITLTLFYLNLGHISWRLSFWFILLLLGLLVIKPTLYKKQFIYSWEERIKDGI
                     IIVSLMGVLFYIAGLLFPIRAHITGGSIERLHYIIAWEPIALATLILTLVYLCLVKIL
                     QGKSCQIGDVFNVDRYKKLLQAYGGSSDSGLAFLNDKRLYWYQKNGEDCVAFQFVIVN
                     NKCLIMGEPAGDDTYIREAIESFIDDADKLDYDLVFYSIGQKLTLLLHEYGFDFMKVG
                     EDALVNLETFTLKGNKYKPFRNALNRVEKDGFYFEVVQSPHSQELLNSLEEISNTWLE
                     GRPEKGFSLGYFNKDYFQQAPIALVKNAEHEVVAFANIMPNYEKSIISIDLMRHDKQK
                     IPNGVMDFLFLSLFSYYQEKGYHYFDLGMAPLSGVGRVETSFAKERMAYLVYHFGSHF
                     YSFNGLHKYKKKFTPLWSERYISCSRSSWLICAICALLMEDSKIKIVK"
     gene            complement(6326..7069)
                     /locus_tag="VBC94_001718"
     CDS             complement(6326..7069)
                     /locus_tag="VBC94_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6979974.1"
                     /translation="MTENWLHTKDGSDIYYRVVGQGQPIVFLHGNSLSSRYFDKQIAY
                     FSKYYQVIVMDSRGHGKSHAKLNTISFRQIAVDLKDILVHLEIDKVILVGHSDGANLA
                     LVFQTMFPDMVRGLLLNSGNLTIHGQRWWDILLVRIAYKFLHYLGKLFPYMRQKAQVI
                     SLMLEDLKISPADLQHVSTPVMVLVGNKDIIKLNHSKKLASYFPRGEFYSLVGFGHHI
                     IKQDSHVFNIIAKKFINDTLKGEIVEKAN"
BASE COUNT         2546 a         1327 c         1082 g         2126 t
ORIGIN      
        1 attattcaat ttcggcatca agatatcatt attctcgaaa ataataaatg taatttgata
       61 atatcaaact attactattt actaaccatt atttccaata tcgttttaaa tgcagtagaa
      121 gctattgata aacaaaaaaa gggtacaatc agtgttcaca cagaagaatt agaagatttt
      181 attaaaattg agattagtga taacggacca ggaatacctg ataaaatgaa acacatgatt
      241 ttcaaacctg gattttcgac taaatttgat gctaatggtg atatctatcg aggaatcggg
      301 ctatcccacg tgcgtatttt aatggaagag caatatcaag gaacgataac tgtttgtcct
      361 aatcagccaa atggaacaac atttacactg ttattccgta aaaatcgatt taagttagga
      421 gataatacgt gaaattctat attattgatg atgaccccac tatcactatg attttacaag
      481 atattattga agaagatttc aataatacag tagtcagagt gaataatgtc tccagtaaag
      541 catacaacga attgctgatt gcagatgttg atatcgtatt gattgattta ttaatgccaa
      601 tattagacgg tgtaacatta gtccaaaaaa tctataaaca gcgttccgat ttaaaattta
      661 ttatgatttc ccaagtcaaa gataatgatt tacgacaaga agcatataaa gcaggaatcg
      721 aattttttat taataaaccc attaatatta ttgaagtaaa aagcgtcgtg aaacgtgtca
      781 ctgatactat cgaaatgcaa aaaaaattaa atacaatcca aaatctccta gaaaatacgc
      841 catcatatca aaagcctata acgactagta acctcactaa aatccgttca atactcagtt
      901 atttaggtat tacttcagag acagcctaca cagatattct taacatctgt gaacttttat
      961 taaaacaaga attaaatttt gcccaatttg attttcaaaa agaactttct atcgatgaac
     1021 accaacaaaa aatcatttta caacgtattc gtcgtgccgt taaaaaagct atgataaata
     1081 tggctcatct gtacattgat gattttgaaa atgaattaac actgcaatat gctaatgcat
     1141 tgtttggatt tcagaatatc cataatgagg cacaacttat tcaaggaaaa agcatgtatg
     1201 gtggtaaaat ttcacttaaa cgttttttcg atgaacttat tttacagagt aaaacgtttt
     1261 aagacttgtt taagcatggc cttatatact ataaccatcc taaaactttt cttttcataa
     1321 atctccaaca aacaccttgt ctaccgcaag gtgtttgttt ttgcttctag tttatcgttt
     1381 ctgacaattt aaatgtcaga tagttgtttg aatgtctact ttatgctaat ataaccctaa
     1441 tatcatacga cctacactcg ttacgcacta gaaagtaaat tacatgattt catttgaaaa
     1501 tgttagtaaa tcatatggtg atcataccat cattgataat attagttgcc atattcaaag
     1561 aggagaattc tttgtccttg tgggggccag tggcagtggt aaaactacca tcctcaagat
     1621 gattaatcgt ctcatcgaac caagccaagg cgctattact ttagatgggg aaaacattac
     1681 atcacttgat ttgcgacaat tacgcttaga aactggttat gtcttgcaac aaattgcact
     1741 tttccctaat ttaacagttg gagaaaatat agaacttatc cctgaaatga agggctggtc
     1801 aaaaggtgac caaaaaaaag ccgctagcga ccttcttgat aaggtgggtt tacctgctaa
     1861 ggactacttt aatcgctatc ctcatgagct ctctggtggg gaacaacaac gcataggtat
     1921 cctacgagca atcgttgcta aacctaaggt actacttatg gatgagccat tttctgcttt
     1981 agaccctatc tcaagacgtc aattacaaga tatcaccaaa caattacagt ctgaattggg
     2041 tattacatta gtgtttgtca ccaatgacat gaaggaagct atgcgtcttg cagatagaat
     2101 ttgtgttatt aaggaaggta agatagtgca attggataga ccagaaataa ttcaaaataa
     2161 tccctctgac caatttgtta gaaccctatt tgaagaggag aactgagtat gacaacacta
     2221 atcactacct ttcaagagcg ttttggagat tggactcaaa gtcttattga acacctccag
     2281 ctatcactac taacacttat tttagcaact ttaattgcta ttccactcgg tatcatcatt
     2341 agtcattata aaaaaatcag tcatgttgtc cttcagataa ctgggatatt tcaaacaatt
     2401 ccttctttag ccttgcttgg tttatttata ccttttatgg gaattggaac tgtaccagct
     2461 gtcgttgcac taattattta cgctctattt ccaattttac aaaatactgt aacagcactg
     2521 atgcaaattg atgctaatct cattgaggca gcaaccgctt ttggaatgac acgttgggaa
     2581 aggcttaaaa agtttgagtt agctttaagt atgcctgtta tcatctcagg cattagaact
     2641 gcctctgtta tgattattgg tacagctaca ttagctagtc ttatcggagc tggtggcctt
     2701 ggatcattta ttttgttagg gattgatcgc aacaatcctt ctctaattct cataggggca
     2761 atctcttctg ctgtcttagc tattatattt agtggtttaa ttggattact agaaaaggct
     2821 agattacgaa ccatagccgt ttctggtata cttttactag caggcttagg actatcctat
     2881 gctccaaaat ggatgcctgg aacaaatact gcaacgataa ctgttgcagg gaagttagga
     2941 actgaaccag atattttaat taatatgtac aaagaactta ttgaggatca gacagatatc
     3001 aaagttaagc ttaaacctaa tttcggaaaa acaactttct tatatcaagc cctaaaatct
     3061 ggtgatattg atctttaccc agagtttaca ggtactatta catcaagttt acttaaaaat
     3121 cctcctaaag taagtaataa ccctaaacag gtttacaact tagccaaaaa cggtatttta
     3181 aaacaagata agttgtcact tttgtcacct atggcttatc aaaacaccta cgctgtagct
     3241 gttaaaaaag attacgctga agctaatcaa ctcaaaaata tcagcgacct aaaaaaatta
     3301 gataaactaa aagctggttt cactttagaa tttaaggata gagaggatgg gagtattggt
     3361 ttacagaaac attatggttt aaaccttgat atctcaacct tagaacctgc tttacgatac
     3421 caagctatca atagtaaaga tgtcaatatt attgacgctt actctaccga tagtgaatta
     3481 atccagtatc aacttcaaat tctcaaagac gataagcatc tatttccgcc atatcaagga
     3541 gcaccgctac taaggcaaga tacgattaaa aaataccctc aagtaaaaaa agctctcaat
     3601 aaacttgctg gtcatatcac tgaaaaagaa atgcaagaaa tgaactacca agtagctgta
     3661 aagcataaat cagcagctac agtagctaaa caatatttaa aagcacatca tattataaag
     3721 tagattcaag caatcaatgg tattaaatga tttaaaaaga gcatgtcgca tgacatgctc
     3781 tttttaattg ccaaataaag cttatttaac aatcttaatt ttactatctt ccattaatag
     3841 ggcacaaata gcacaaatta accaggacga acgagaacaa gaaatataac gttccgacca
     3901 caatggtgta aacttcttct tatacttgtg taaaccatta aatgagtaga aatgactacc
     3961 gaaatgatag acaagatacg ccattctctc tttagcaaaa cttgtttcaa cgcgaccaac
     4021 tcctgataaa ggtgccatcc ccaaatcaaa atagtggtat cccttctctt gataataaga
     4081 gaataatgat aaaaagagga aatccataac gccattcgga attttctgtt tatcgtgacg
     4141 cattaaatca atagagataa tactcttttc atagtttggc ataatattag caaaagcaac
     4201 aacttcgtgt tcagcatttt tcaccaaagc tattggggct tgttggaaat aatctttatt
     4261 aaaatatcct agtgagaaac ctttttcagg acgtccttct aaccaagtat tagaaatctc
     4321 ttccaaacta tttagtagct cttgactatg tggcgattgt acaacttcga aatagaaacc
     4381 atccttttca actctattta gggcatttct gaaaggtttg tacttattcc ctttaagagt
     4441 aaacgtttct aaattaacta aagcatcctc accaactttc ataaagtcaa aaccatactc
     4501 atgtaaaagt agtgtcaact tctgtccaat actgtaaaaa acaaggtcat agtctagctt
     4561 atcagcatca tcaataaacg attcaatagc ttcacgaata taagtgtcat caccggctgg
     4621 ttcccccata ataagacatt tattattgac aattacaaat tggaacgcaa cgcaatcttc
     4681 tccatttttt tggtaccagt agagcctttt atcatttaaa aaggctaaac cgctatccga
     4741 agaaccaccg taagcttgaa gtagtttttt ataacgatcc acattgaaca catcaccaat
     4801 ctgacaagat tttccttgta aaatcttaac caaacataaa taaacgagag taagaatcaa
     4861 cgtagccaat gctatcggct cccatgctat gatataatgc aggcgttcaa tactaccacc
     4921 tgtaatatga gccctgatag ggaatagtag tcctgcaata taaaatagaa ctcccattaa
     4981 actaacgata atgattccat ccttaatacg ctcttcccag ctataaataa attgtttttt
     5041 atagagagtt ggcttaatga ctaataagcc taacaatagt aaaataaacc agaaagatag
     5101 tcgccaacta atatgaccta aattaagata aaacaatgtc aaagtaatcc agataatagc
     5161 aattggaaaa gcatttttca ctttttgatc aatagttcta gctaagagaa taaaacaaac
     5221 ccccagcaat aaacctggaa actgccataa catttgttct tgtaatgggt ccaagcctag
     5281 cttctgcaac cacataatat aagtaatatt ttcaaaaaat gctgttgaaa atattaagaa
     5341 tgcacctaaa atacgcatca aatggctcac catggtttgt agaacagttg atactaactc
     5401 tttcgggaca ttttcataac gttgatttat ttgactacct aaataatgga taaagaaata
     5461 gatacctgca aagaatggaa taatatagta ggctaaacga taaagtaata accatgcaac
     5521 cacagtttct ttaggtagtc cctcggcagc aaaccctgta aatagaacta attcaaaact
     5581 tcctaatcca ccgggaataa gggatacaat cccgacggca caaccaatac aaaatagtgg
     5641 taaaatttta taaactggta gatgaatgcc cattaaataa ccgataatta taaacgctgc
     5701 ggctgcacat ccccattcaa aaaaagaaac aatacctaat tttatacgag tactagatgg
     5761 catatctccg aaataatggc ttcctttatg accagaaatc caataaataa caggaaaata
     5821 catactagca ccaattaata ccaaataata gtaatcaaca ctagctttgg catgaaaaat
     5881 atggctcatg attaaggcaa tcacactaat aaatgacaga ccagaaataa gatagggtaa
     5941 aaaacgagtc acttctctta ggtcactctt ctcttgacct tttttaccat aaaaagccat
     6001 gcgcaaccca atatcgatta agccaccgaa tcctgacaag ttattaaaag tgttgataca
     6061 ccaactagtc tgtaaaatat accttttgct tttatctgta cgtaaaagtc cattcagtac
     6121 aaagtcatat ccggttgtcg gtaagacagc gataaccccc acaaggataa ttaagaaaag
     6181 attcattgga gataattgcc caaaaacact ctttagttgc tcaacagaaa tagttcgttt
     6241 caaatgaatc atttcaacaa taataagcac taaaacagat ataaaaaata caattttaat
     6301 cacagaagtc aatgatttga ctttttcaat tagctttttc aacaatttct cctttcaacg
     6361 tatcgttgat aaactttttt gcaataatat taaaaacatg ggaatcttgc ttaataatgt
     6421 gatgcccaaa gccaactaaa gaataaaact ccccccttgg aaaataagaa gcaagtttct
     6481 tagaatgatt taacttaatt atgtccttat ttccaaccaa aaccattaca ggagttgaca
     6541 catgctgtaa atcagctgga ctaatcttca aatcctccaa cataagcgaa ataacttgag
     6601 ctttttgcct catatacgga aagagtttcc ctaaatagtg aaggaattta taggcaatcc
     6661 ttactaaaag aatatcccac catcgctgac catgaatagt caggttccct gaattaagca
     6721 aaagccctct aaccatatct ggaaacatcg tttgaaaaac taaagctaaa ttagcaccat
     6781 cgctatggcc taccaatata actttatcaa tctctaaatg aactaagata tcctttaagt
     6841 caactgctat ttgcctgaaa ctaatggtat ttagctttgc atgacttttg ccatgccctc
     6901 tactatccat aacaataact tggtaatact tagaaaaata tgctatttgc ttatcaaaat
     6961 agcgactact taagctattg ccatgtaaaa aaacaatcgg ttgaccttga ccaacgacac
     7021 gataataaat atctgaacca tctttagtat gtaaccagtt ctcagtcata accaaagtca
     7081 c
//
LOCUS       DATROQ010000091         6997 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.092, whole genome shotgun sequence.
ACCESSION   DATROQ010000091 DATROQ010000000
VERSION     DATROQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.092"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            5..532
                     /locus_tag="VBC94_001719"
     CDS             5..532
                     /locus_tag="VBC94_001719"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEO6979975.1"
                     /translation="MEKKLLRKEVLITLKSQPQAYKSEVDSKLLEAFIKTKAYQNSCV
                     IATYLSFDYEYNTQLLIKQALCDGKRVLVPKTYPKGKMIFVDYQKDNLRATPFGLLEP
                     VNDRAVEKASIDLIHVPGLIFNNKGFRIGYGAGYFDRYLSDFEGDTISTIYRCQRQDF
                     VEEKHDVAVKEVLCL"
     gene            523..1200
                     /locus_tag="VBC94_001720"
     CDS             523..1200
                     /locus_tag="VBC94_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEO6979976.1"
                     /translation="MSMKKFAKEYPTTVLLVGLTTLVFLLMQLTYGSQAESSQVIFQF
                     GGIQGDYLKAYPTNLWRLISPIFVHIGWEHFLLNGLALYFVGQMGESIWGSLRFLILY
                     ILSGLMGNIFTLFFTPHVVAAGASTSLFGVFSAIAIAGYFGKNPYLKQVGKSYQVMIL
                     LNLFFNIFTPGVSLAGHVGGLVGGVLVAIFLTKQNGSLLFKTWQSILALMIFIIVSIS
                     LIGLSLV"
     gene            1332..2375
                     /locus_tag="VBC94_001721"
     CDS             1332..2375
                     /locus_tag="VBC94_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO6979977.1"
                     /translation="MNRKIIGLTLLSLSVLTLTACGNRSDKSANKSDIKVAMVTNQGG
                     VDDKSFNQSAWEGLQKWGKKKGLTKGNGFDYFQSSNESDHANNLDTAASSGYNLIFGI
                     GFGIHDTIEKVSENNKDVKYVIVDDIIKGKENVASVTFADNEAAYLAGVAAAKTTKTK
                     TVGFIGGMEGVVVKRFEAGFKAGVKSIDPAIKVAVSYAGSFTDAAKGKTIAATQYATG
                     VDVIYQAAGGTGAGIFSEAKTENETRKESNKVWVIGVDRDQSQEGNYVSKDGKKANFV
                     LASTIKEVGKSLQSVAELTEKKQYPGGKVTVFGLKDSGVDIKEHQLSSEGSVAVKKAK
                     EGIVSGKIQVPMK"
     gene            complement(2466..3365)
                     /gene="galU"
                     /locus_tag="VBC94_001722"
     CDS             complement(2466..3365)
                     /gene="galU"
                     /locus_tag="VBC94_001722"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEO6979978.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKNELLKLVDETTGIRLHFIRQ
                     SHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNNKVIPLTKQLINDFEATHASTIA
                     VMEVPHEDVSAYGVIAPQGEGVNGLYSVNTFVEKPSPEEAPSNLAIIGRYLLTPEIFN
                     ILETQKPGAGNEIQLTDAIDTLNKTQRVFARKFTGDRYDVGDKFGFMKTSIDYALQHP
                     QVKDDLKKYIIDLGKSLEKTSKK"
     gene            complement(3402..4418)
                     /locus_tag="VBC94_001723"
     CDS             complement(3402..4418)
                     /locus_tag="VBC94_001723"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO6979979.1"
                     /translation="MTKQKIAVLGPGSWGTALAQVLNDNGHEVRLWGNVVEQIEEINT
                     NHTNQRYFKDITLDSKIKAYTNLEEAINNVDSILFVVPTKVTRLVAKQVASLLKHKVV
                     IMHASKGLEPGTHERLSTILEEEISEQYRSDIVVVSGPSHAEEAIVRDITLITAASKD
                     IEAAKYVQKLFSNHYFRLYTNTDVVGVETAGALKNIIAVGAGALHGLGYGDNAKAAII
                     TRGLAEITRLGVQLGADPLTFSGLSGVGDLIVTGTSVHSRNWRAGDALGRGEKLEDIE
                     KNMGMVIEGISTTKVAYEIAQNLNVYMPITEAIYKSIYEGANIKDSILDMMSNEFRSE
                     NEWH"
     gene            4588..4917
                     /gene="rnpA"
                     /locus_tag="VBC94_001724"
     CDS             4588..4917
                     /gene="rnpA"
                     /locus_tag="VBC94_001724"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEO6979980.1"
                     /translation="MKKTYRVKSDKDFQMIFSRGKNVANRKFVIYYLEKEQKHFRVGI
                     SVSKKLGNAVVRNAVKRKIRHVLLSQKTALQDYDFVVIARKGVEELDYQALEKNLIHV
                     LKIAGLI"
     gene            4930..5745
                     /locus_tag="VBC94_001725"
     CDS             4930..5745
                     /locus_tag="VBC94_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO6979981.1"
                     /translation="MKKKLKTFSLILLTGSLLVACGRGEVSSHSATLWEQIVYAFAKS
                     IQWLSFNHSIGLGIILFTLIIRAIMMPLYNMQMKSSQKMQEIQPRLKELQKKYPGKDP
                     DSRLKLNDEMQSMYKAEGVNPYASVLPLLIQLPVLWALFQALTRVSFLKVGTFLSLEL
                     SQPDPYYILPVLAALFTFLSTWLTNKAAVEKNIALTLMTYVMPFIILVTSFNFASGVV
                     LYWTVSNAFQVFQILLLNNPYKIIKVREEAVRVAHEKEQRVKRAKRKASKKRK"
     gene            5753..6574
                     /locus_tag="VBC94_001726"
     CDS             5753..6574
                     /locus_tag="VBC94_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEO6979982.1"
                     /translation="MVLFTGATVEEAIEKGLQELNISRLRAHIKVVSREKKGFWGFGK
                     KPAKVEIEGITDEVTDINESVALKNIKNVPSSVDVVEEYIEEVDETLEKEDVSQPELP
                     KIDDKNVVTTSEAIEKIDLLPNIEVAAAQVTKYVENIIYEMDLDATIETTTSKRQINL
                     QIETPEAGRIIGYHGKVLKSLQLLAQNYLHDRFSKSFSVSINVHDYVEHRIETLIDFS
                     KKIARRVLETNEPYHMDPMSNSERKTVHKTIATIEGVESYSEGNDPNRFVVVTKK"
     gene            6914..>6997
                     /locus_tag="VBC94_001727"
     rRNA            6914..>6997
                     /locus_tag="VBC94_001727"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2346 a         1083 c         1353 g         2215 t
ORIGIN      
        1 atatatggaa aagaaattat taagaaaaga agttttgata accttaaaat ctcaacctca
       61 agcttacaaa tcagaagttg actctaaact tttagaggct tttataaaaa ctaaagctta
      121 tcaaaatagt tgtgtaattg ctacttacct atcttttgac tacgaataca atacacaact
      181 tcttattaaa caagctttat gtgatggcaa acgtgtcctt gttcccaaaa cttatccaaa
      241 aggtaaaatg atttttgtgg actatcaaaa agataacttg agggcaacac cgtttggctt
      301 attagaacct gttaatgata gagctgttga aaaagctagt atagacttaa ttcatgtgcc
      361 cggacttatc tttaacaata aaggttttcg tataggttat ggtgcaggtt attttgatcg
      421 ctatttgagt gattttgaag gtgatactat tagtactatt tataggtgtc agagacagga
      481 ttttgtagaa gagaagcatg atgtagcggt taaggaggta ttatgtctat gaagaagttc
      541 gctaaagagt atccgacgac agtactcttg gtcggtctga caacccttgt ttttttattg
      601 atgcaactaa cttatggaag tcaagcagag tctagccaag ttatttttca atttggtgga
      661 atacaaggtg attatctaaa agcatatcca acaaatctat ggagactaat aagtcctatc
      721 tttgttcata ttggttggga acatttttta ttaaatggtt tagctctgta ttttgtggga
      781 caaatggggg aaagtatttg gggaagtttg agatttctaa ttctctatat tttatctggc
      841 ttaatgggta atatttttac gctatttttt actccacacg tggtggcagc aggagcctca
      901 acgtctttat ttggtgtttt ttctgctata gcaatcgcag gttattttgg aaaaaatcct
      961 tatttaaaac aagttggaaa atcatatcaa gtgatgatct tgttaaattt attttttaat
     1021 atcttcacac ccggagtgag tttagcaggc catgttggtg gtttagttgg tggagtattg
     1081 gtggctattt ttttgacaaa acaaaatggt tctttattat ttaaaacttg gcaaagtatt
     1141 ttagctttaa tgatatttat tattgtatcg atcagtttaa taggtctatc gttggtataa
     1201 ttctacggct atagtctcta aaaccaaact ttctttggga aaaatagaat aaagtttgct
     1261 aaatgatttt tttatgttat gatagaaatc tgtttttatg tgactataac ttattaggag
     1321 gttcattctc ggtgaacaga aaaattattg gcttaactct actatcttta tctgtactaa
     1381 cattaacagc atgtggtaat cgttcagata agtctgcaaa taaatcagat atcaaagttg
     1441 cgatggtaac taatcaaggt ggagtagacg ataaatcgtt taatcaatca gcttgggaag
     1501 ggctacaaaa gtggggaaaa aagaaggggt taacaaaagg taatggcttt gattatttcc
     1561 agtcatctaa tgaatcagat catgctaata accttgatac ggcagcctct agtggctata
     1621 atttaatttt tggtattgga tttggtattc atgatactat tgaaaaagta tcagaaaata
     1681 ataaagatgt taaatacgtt attgtagatg atatcattaa aggtaaagaa aatgtagcta
     1741 gtgttacttt tgcagacaat gaagctgctt acttagctgg agttgctgca gctaagacaa
     1801 ctaagacaaa aacagttggt tttatcggag ggatggaagg cgtcgttgtt aagcgatttg
     1861 aagcaggatt taaggctgga gtaaaatcaa ttgatccggc tattaaagtt gcggtatctt
     1921 atgcaggctc atttacagat gcagctaaag gtaaaacgat tgctgccaca cagtatgcaa
     1981 caggtgttga tgttatttac caggctgctg gtggtacagg agcaggaatt tttagtgagg
     2041 ctaaaactga aaatgagacc cgcaaggaat ctaacaaagt ctgggtaatt ggtgttgaca
     2101 gagatcaaag tcaagaaggc aattatgttt caaaagatgg taagaaagca aactttgttc
     2161 tggcatctac tataaaagag gtggggaaat ctctccaatc tgtagcagaa ttaacagaga
     2221 aaaaacaata tccaggaggg aaagtaacag tttttggtct aaaggatagt ggtgtagata
     2281 ttaaagagca tcaattgtct agtgagggat ctgttgctgt caaaaaagct aaagagggga
     2341 ttgtttcagg gaaaatacaa gttcctatga aatgataaaa gtgtggagga acaataagtt
     2401 cttcgtcaag tacaataaaa aagtttccta aataggaaac ttttttattg tacttgactc
     2461 atattttatt tcttagatgt tttttctaga gattttccta aatcaatgat atatttcttc
     2521 aagtcatctt taacttgtgg gtgttgaaga gcataatcaa tagaagtttt cataaaacca
     2581 aacttgtcac caacgtcata acggtcccct gtaaattttc tagcaaaaac acgttgcgtt
     2641 ttatttaaag tatcgatagc atcggttagt tgaatctcat ttcctgcacc aggtttttga
     2701 gtttctaaaa tattaaaaat ttctggagtt aacaagtagc gaccaatgat tgccaaatta
     2761 cttggagcct cttcgggaga aggtttttca acgaaagtgt taacgctata tagaccatta
     2821 actccttctc cttgtggagc aataacacca taagcagaaa catcttcatg tggaacttcc
     2881 ataacagcaa ttgttgatgc atgagtagct tcaaagtcat taattaattg tttggtgaga
     2941 ggaataactt tattattggt aatatccatt agatcatcac ctagcataac aacaaaaggt
     3001 tcattaccaa cgaaggcttt tgcttgtagc acagcatctc caagcccacg aggatgactt
     3061 tgtcgaataa aatggagtcg aatgccagtt gtttcatcaa caagtttgag aagttcgttt
     3121 ttccctttct cttttaggtt atattctaat tcaaaatttg aatcaaagtg gtcttctata
     3181 gaacgttttg atttaccagt tacaactaaa atatcttcaa ttccagattt tagtgcttct
     3241 tcaacaataa attgaatagt tggtttatca acgattggaa gcatttcttt agctaaagct
     3301 ttagtagcag gtaaaaaacg tgttcctaaa ccagcggcag ggataactgc ttttctgact
     3361 ttcatatata gctcctttaa atagtattaa tataaacgat attagtgcca ttcattctct
     3421 gaacgaaatt cattggacat catatctaaa atgctatctt tgatattagc cccttcatat
     3481 attgatttat aaatagcttc tgtaatgggc atgtaaacat ttaagttttg ggcgatttcg
     3541 taagcaacct tagttgttga gataccttca ataaccatcc ccatattttt ttcaatatct
     3601 tcaagtttct caccacgtcc taaagcatct cccgctctcc agttgcgaga atgaacagat
     3661 gtaccagtca caattaaatc tccaactccg gataaaccac tgaaagtgag agggtcggcc
     3721 cctaattgta caccaaggcg tgttatctca gctaaaccac gcgtaataat agctgcttta
     3781 gcattatcac cataaccaag accgtgtagt gctccagcac caacagcaat aatatttttt
     3841 agagcaccag ctgtttctac tcctacgaca tcagtgttag tataaagtct aaaataatga
     3901 ttactaaaca atttttggac atacttagct gcttcaatat cttttgaagc agcagtaatc
     3961 aaagtaatat cacgaacgat ggcttcttca gcatgactgg gacctgaaac aacaacgata
     4021 tcactacgat attgttcaga gatttcttct tctaaaattg tagaaagtcg ttcatgagtg
     4081 ccaggttcta gccccttaga agcatgcata attacaactt tatgctttaa aagactagcc
     4141 acttgtttag caacaagtct tgtaacttta gttgggacga cgaatagaat gctgtcaaca
     4201 ttattaatcg cttcctcaag attagtgtag gcttttattt tggaatctaa ggtaatatct
     4261 ttgaagtaac gttgattagt atggttagta ttaatctctt ctatttgttc aaccacgttt
     4321 ccccataagc gtacctcatg tccattgtca tttaatactt gtgcaagagc agtcccccat
     4381 gaaccaggtc caagaacggc aattttttgt tttgtcatct tattccttct ttctaataat
     4441 aaggcatata gcgctaacat tatatcatac tctaatacac tttgtcttgt ttagtctgtt
     4501 gtcgatgata gtagcattag aaaagaattg agtcttttag gttatttatg gtataataaa
     4561 accaatttag ataaatggag cactattttg aaaaaaacct atcgggtaaa aagtgataag
     4621 gattttcaaa tgattttcag cagaggtaaa aatgttgcta atcgaaaatt tgttatctac
     4681 tatttagaaa aagaacaaaa acactttcgt gtgggaatct ctgttagtaa gaaattagga
     4741 aatgctgttg ttcgtaacgc tgttaaaaga aaaattaggc atgttttact ctctcagaaa
     4801 acagcgctgc aagattatga ttttgtcgtg attgcgcgta aaggtgtaga ggaattagac
     4861 tatcaagcat tagaaaaaaa tcttatacac gttttaaaaa tagcaggact catctagaaa
     4921 ggtataaaat tgaagaaaaa acttaaaaca tttagcttaa tattgctgac aggttcttta
     4981 ttggttgctt gcggacgagg agaagtatct agtcattcag ctacattatg ggagcaaata
     5041 gtttatgcat ttgctaaatc aatacaatgg ctctccttta accattcaat tggtttggga
     5101 ataatcttat tcactctaat aatcagagcg attatgatgc cgctttataa tatgcaaatg
     5161 aaatccagcc aaaagatgca ggaaattcag ccaaggctca aggaattaca aaaaaaatat
     5221 ccagggaaag atcctgatag ccgtttaaag ctaaatgatg aaatgcaatc tatgtataaa
     5281 gctgagggag ttaacccata tgcaagtgta ttacctcttc taattcagtt acctgtccta
     5341 tgggctcttt tccaagcttt gactagggtt tctttcttaa aagtaggaac gtttttgagc
     5401 ctagaattat cacagccgga cccatattat attttacctg tactggcggc cttatttacc
     5461 tttttatcaa cttggttaac taacaaagca gctgttgaaa aaaatattgc tttaactctt
     5521 atgacttatg ttatgccgtt tatcatattg gtaacaagtt ttaactttgc tagtggtgtt
     5581 gttttatact ggacggtgtc taatgctttt caagtatttc aaattctatt attgaacaac
     5641 ccatacaaga ttataaaggt tcgagaagaa gcagtaagag tggcacacga aaaagaacaa
     5701 agagttaaac gtgctaagag aaaagcaagt aagaaaagaa agtaggaaaa acatggtatt
     5761 atttacagga gcgactgtag aagaagctat tgaaaaagga ctacaagagt taaacatttc
     5821 acgattgagg gcacatataa aagttgtatc acgtgagaaa aaaggttttt ggggctttgg
     5881 taaaaagcca gcaaaagttg aaatagaagg tatcaccgac gaagtaactg atattaatga
     5941 atcggtagcg ttaaaaaata ttaagaatgt accgtctagc gtagatgtgg tggaagaata
     6001 tatagaggag gttgatgaaa cgcttgaaaa agaagacgta tcacaaccag aacttccaaa
     6061 aattgatgat aaaaacgttg taactacaag tgaagctatt gaaaaaattg acctacttcc
     6121 taatattgaa gtagcagctg ctcaggtgac taaatacgtt gaaaatatca tctatgagat
     6181 ggatttagat gcaacaatag aaactacgac aagtaagcga caaataaatc tccaaataga
     6241 aacgccagaa gctgggcgca tcattggtta tcatgggaaa gtattaaaat cgttgcagtt
     6301 gttagcacaa aattatcttc atgaccgctt ttctaagagt ttttcagtat ctattaatgt
     6361 ccatgactac gttgagcatc gtatagaaac tttaattgat ttttctaaaa aaatagctag
     6421 acgtgtttta gagactaatg agccttacca tatggatcct atgagtaaca gtgagcgaaa
     6481 aactgttcat aaaacaattg caactataga gggtgttgaa agttattctg aaggtaacga
     6541 tcctaataga tttgtagtag ttactaagaa gtaaaaagag gtttcacctc tttttttctt
     6601 gcgcccgatc tacattttca gaaaatgtag aaaaagtatg aaaaaagagt tgacaaagta
     6661 agagatgatg tagtaaacta aataagttgt ctcgagagag cagtttctag tagcgcgtag
     6721 ttaacaactt aaaaaaagtg gttgacaaag cagaataagt ttgctagaat atagaagttg
     6781 tctcaagaga ggcaaagacc tttgaaaact gaacaagacg aacgaatgtg cagggtggat
     6841 ttacattgta aatccgtcaa tgacaaaaaa caataaatct gtcagcgaga cagaaaagag
     6901 caaagctcaa acttttaatg agagtttgat cctggctcag gacgaacgct ggcggcgtgc
     6961 ctaatacatg caagtagaac gctgaggttt ggtgttt
//
LOCUS       DATROQ010000092         6933 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.093, whole genome shotgun sequence.
ACCESSION   DATROQ010000092 DATROQ010000000
VERSION     DATROQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.093"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            14..700
                     /locus_tag="VBC94_001728"
     CDS             14..700
                     /locus_tag="VBC94_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HEO6979983.1"
                     /translation="MIEFFSNIRTEIPQMPLLIHSLILSVLPFLMWLTLVNRDKPLYK
                     TIWSILLGLQLITIYTWFFWAKLPLSESLPLYHCRIGMFVVLLARPRILKDYFALLGV
                     VGGVLAMIHPDFYPYQFLHVTNIFFFIGHFALFVLSLLHLMTQSNLDKLNPKLIIQLT
                     LLINMSLIFINLLTGGNYGFMMKTPILGITNPFLNLLIVTTLLSFLVLFVKQIFQKVR
                     MRASFKTEES"
     gene            880..2417
                     /locus_tag="VBC94_001729"
                     /pseudo
     CDS             880..2417
                     /locus_tag="VBC94_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            2594..4138
                     /locus_tag="VBC94_001730"
     CDS             2594..4138
                     /locus_tag="VBC94_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEO6979984.1"
                     /translation="MTLQDEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLDLLEELEEVLG
                     IASYPMNWPIGMGKSFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VIDPLAKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPELFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWINSDDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     KLEEKM"
     gene            4224..4604
                     /locus_tag="VBC94_001731"
     CDS             4224..4604
                     /locus_tag="VBC94_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEO6979985.1"
                     /translation="MGIFSGLMGNASQVDTDKVENQLSDILIPGEQVDLAYTLIRDLI
                     VFTNYRLILVDKQGVTGKKVSYNSIPYASISRFTVETSGHFDLDAELKIWISSAIEPA
                     EVLQFKNDRNIVSIQKALAIAVLK"
     gene            4798..5532
                     /locus_tag="VBC94_001732"
     CDS             4798..5532
                     /locus_tag="VBC94_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6979986.1"
                     /translation="MIIELKSVGKIFPKSQFRLDDISFNIAEGEIVGLIGSNGTGKST
                     ILKMINGLIPYDKGNIYYQGKEVKSFSDNKLRQMRKDIAYIFQNHNLLAGESVYYHLA
                     LVYKLNHQKVNHDAINDILDFLGLMDLKQVKCHSLSGGQQQKVAIAMAVLQKPKLILC
                     DEISSALDTNSEKEIFNLLSDLREKYGISILMIAHHLSLLKQYCDRVMILDHQTIVDT
                     VVPVKATLNQLKSNYVDQVKEFLLHD"
     gene            5525..6187
                     /locus_tag="VBC94_001733"
     CDS             5525..6187
                     /locus_tag="VBC94_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6979987.1"
                     /translation="MIDIIEKHGEGILKSFWETNLMLGLTLILCFLIAFPTGILLFSL
                     RKSYLIKHSLAYQLLNLFLGTLRSVPFLIFIFILIPLNRLIFGTSFGTIAAILPLTLV
                     SVSLYARYVEQALLNIPQVVVDRALSLGANKRQIIYYFLIPSIKIDLVLSFTATAISI
                     LGYSTIMGVIGAGGLGEYAYRFGYQEYDYPVMYLIVVLFIIYVFILQSLGYFIANRYS
                     RK"
     gene            6203..>6933
                     /locus_tag="VBC94_001734"
     CDS             6203..>6933
                     /locus_tag="VBC94_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979988.1"
                     /translation="MKKLLSLTCLIMMSLCLVACTKQAMSSKQIKDKNSKEKVITVAT
                     YSKPTSTFLDLIKDNVKEKGYTLKVVMVSDYIQANIALENKEHDANLLQHEFFMSIFN
                     KENDGHLVSITPIYHSLAGFYGQHLKNIAELKDGAKVAIPSDPANMTRALLLLQEKKL
                     ITLKNTSKKTKAIEDIITNPKKLRIEPVALLNLNQAYFEYDLVFNFPGYVTKINLVPK
                     RDRLLYEKKPDIRFAGALVAREDNK"
BASE COUNT         2273 a         1047 c         1317 g         2296 t
ORIGIN      
        1 agataatact aaaatgatag aattcttttc taatatcaga acagagattc cgcagatgcc
       61 tttacttatc catagtttga ttttatctgt cttacctttt ctgatgtggc tgactttggt
      121 taatagagat aagcctttgt ataaaactat ttggagtatc cttttaggac ttcagttaat
      181 tacgatttat acttggtttt tctgggcaaa attgccttta tctgaaagtc ttccccttta
      241 ccattgtcga ataggcatgt ttgtcgttct cttagcgagg cctagaatat taaaggatta
      301 tttcgcattg ttaggagtag taggtggagt attggcaatg attcacccag acttttaccc
      361 ttaccagttt ctacatgtga ctaatatttt cttctttatt ggacattttg ctttatttgt
      421 ccttagttta ctacatctga tgacacagtc caatttggac aaattaaatc caaaattgat
      481 tatacaacta acgcttctca ttaatatgtc tttgattttt attaacctct tgactggggg
      541 aaattatggc tttatgatga agacaccaat actaggaata acaaatccct ttttaaacct
      601 tttgatagtg acaactctat taagttttct cgtcctattt gttaaacaaa ttttccaaaa
      661 agtgagaatg agagctagtt ttaaaacaga agaatcttaa ttattttaat attattatat
      721 tatctagcta ttaaaaaata aatagctgga taatgttttt catttttagc tatttttatg
      781 attgaccgct aattattaat ctgctaagat atagtacgta ataaaaactt aaccggttaa
      841 ttattttttg aaatatattt tttctggagg aaaatagtaa tgaataaatc attcaatacc
      901 aaattaggtt tggtaactgt agcggcatta tcaggtattg tattaacgtc tcaattacct
      961 gtgaacgcta aagctgagac accagtaatg gcagcaagtg cacaacaagg tttccgtttt
     1021 gtagcaaccg ttgtagatag tcagacgcgt gtcttgccgg gaaaattggt aactttgtca
     1081 gaggtgacaa ctggtcagcc taaaattatt gcaagtgtaa aaacgaatga tgccggacaa
     1141 gctattttta ataatctccc tataaaacgt aaccttagtg tgtctgttga tggacaagta
     1201 aaaggatata ctattcgaac tgacgtagca ggctctagta aagcagctag ttttacagca
     1261 acaggagtag ggacaaatga gccaacgtat tcaaaaaaaa ccatagacat tacagtgaga
     1321 gaccaaaatg ctgaacctgt ttcaggcagg acagttacct taaaaacaca tgctggtcgt
     1381 gagattgcta gcttggtttc tggtgacaat ggattaactc gctttactga tcgtttacta
     1441 gatggaacat tctaccaata ttttgttgat gggaaaaaat tggtgatatt gtacctggag
     1501 aatcaagaag tgcatatgtt aatgttgcac ctaaaaaaga ttattttacc tttacagtca
     1561 ctgctttaga taaagatgga cttgttgtta agggaaaaga agtaacgttg actgatatta
     1621 ctgatggtaa agcagtagcg cttgcttctt taaaaacgaa tgataatgga caagctatct
     1681 ttaccaacct accattatca agaaatatca gtgtttcaat tgatggaaaa tcaaagggct
     1741 atactcttcg aactgatgta gcaggctctc ataaagcggc tgcattctat gtagatggta
     1801 aaggtaccaa agcacctcaa tttagtgcag aagcagcggt tgtaactatt tacgatgcta
     1861 atggaaatac tatagcaaac caagaagtga cattaactaa ttcaaatggg acaattgttg
     1921 caaaaggttt gactgaaaaa gatggcaaag caaggttcgc caataaatta atgggaggaa
     1981 cgttatacaa tatttttgta aatggtattg agatgcctaa aactgccctt gttggtagtg
     2041 atgtcagtgt ttttctaaca gataaacaaa ttaagaaaga aaaacctgtt actcctgata
     2101 aattggataa agaaatgaac caagagaagg aaaaggagct cttagatact ccaaaaccag
     2161 taccacctac ttcgaaacca acagcaccaa ttaaaaataa aaaggacgat tcccctaaga
     2221 agtttacgaa aagtcaacct tctagtttga agtcaagtgt taaggctata ggtgctaagc
     2281 caaaagctat gacagcctta gctaaaaaat tgcctaaaac tggtgatcaa gccattagta
     2341 ttttaacttt ggttggtttt attgtaacag ggttagcgat tatgttaggc ttctttaaaa
     2401 aatcacgatc aaaataattt attttagaag cttgggtctt tagacacagg cttctcttct
     2461 ttcttcggaa atagattgaa cttgaagaat gttgttaaat tcgctataat aataaagcta
     2521 tagacaagat tattgtttca gttgagacta ctgaaaataa tactaagaaa tggataaaag
     2581 ctaaggattg aatatgacat tacaagacga aatcaaaaaa cgccgtactt ttgccatcat
     2641 ctctcacccg gatgctggta agacgactat tactgagcaa ttattatatt ttggtggtga
     2701 aattagagaa gcagggacag taaaagggaa aaaatcaggt acttttgcaa agtccgactg
     2761 gatggatatt gaaaagcaac ggggtatctc tgttacttca tctgttatgc aatttgatta
     2821 cgcgggtaaa cgtgttaata ttttggatac acctggacat gaggatttct cggaagatac
     2881 ataccggacc ttaatggctg tggatgcagc tgtcatggtt gtagattcgg ctaaaggtat
     2941 tgaggctcaa actaaaaaac tttttgaagt tgttaagcat cgtaatattc cggtttttac
     3001 gtttattaat aaattggacc gtgatggacg tgagccactg gatttgcttg aagagttaga
     3061 agaagtacta ggtattgctt cttatccaat gaattggcca attggtatgg ggaaatcatt
     3121 tgaagggctt tatgatttac ataataaacg tttagaactc tataaaggag acgagcgttt
     3181 tgcaagtatt gaagacggtg atcagctgtt tgccaataat ccattttatg aacaagtcaa
     3241 agaagatatt gaacttcttc aagaagcagg taatgacttt tcagaacaag ctattcttga
     3301 tggtgaccta actccagtat tctttggctc tgcccttacg aactttggag ttcagacttt
     3361 tcttgatact ttcttggagt ttgctccaga acctcatggt cacaagacta cagaaggtaa
     3421 tgttattgac cccttagcta aagatttttc aggttttgtc tttaaaattc aagctaacat
     3481 ggacccacgt catcgcgacc gtattgcttt tgttcgtatt gtttctggtg aatttgagcg
     3541 cggcatggga gttaatttga cacgcactgg taaaggcgct aagctctcca atgttactca
     3601 atttatggca gaatcacgag aaaatgtgac gaatgctgtt gctggggata ttatcggggt
     3661 ttatgataca ggaacttatc aagttgggga taccttaacg gttgggaaaa ataaatttga
     3721 atttgaacca ttaccaactt ttaccccaga attatttatg aaagtatcag caaaaaatgt
     3781 tatgaagcag aaatcattcc acaagggtat tgagcaatta gtacaagaag gagcaatcca
     3841 gctttataag aattatcaga caggcgaata catgctgggt gctgtgggtc aacttcagtt
     3901 tgaagttttt aaacatcgta tggaaggtga atacaatgct gaagttgtta tgacaccaat
     3961 gggtaaaaag actgtacgtt ggattaactc agacgattta gacgaacgca tgtcctcaag
     4021 ccgaaatatt ttagcaaaag atcgttttga ccaaccagtt ttcctatttg aaaatgattt
     4081 tgcacttcgc tggtttgctg ataagtatcc tgatgtgaag cttgaagaaa aaatgtaaaa
     4141 tttaaagtca ctcaaatttg agtgactttt taagtttttt agactaaaat agttataata
     4201 catagaaaag attggagatt aacatgggaa ttttttcggg attaatggga aatgcttcac
     4261 aagtggatac cgataaagtc gagaatcagt tatctgatat tctgattcca ggtgaacagg
     4321 ttgatttagc ttatacttta attcgtgatt tgattgtttt tactaattat cgtttaattt
     4381 tagtcgataa acagggggtg acaggtaaaa aggtttctta taattctata ccttatgctt
     4441 ctatatcacg ttttacagtg gaaactagtg gtcattttga tttagatgct gagttaaaga
     4501 tttggatatc atcagcaata gaaccagcag aagtacttca gtttaagaac gaccgtaaca
     4561 tagtgagtat acagaaagct ttggcgatag ctgttttaaa ataaacaatt tagcaagtgc
     4621 tgctactaag agttatttgt tttaattata acttttaata gtgctaaatt ctcattttca
     4681 aaataaaagt agtgattttt atcactgctt ttattttgaa gtatagtagg cctcaagcat
     4741 atattttgaa aacgctgtat ttttttgata agatattttt ttgaggagta ttaaataatg
     4801 ataatagaat taaagagtgt tgggaaaatt tttccaaaat cacagtttag attagatgat
     4861 atttctttta atatagcaga aggcgagata gttggtctga ttggttctaa tggtactgga
     4921 aagagtacta ttttgaagat gattaatggc ttaatccctt atgataaagg aaatatttat
     4981 tatcaaggaa aagaagtgaa gtctttttct gataataagt tacgtcagat gcgtaaagat
     5041 attgcttata tttttcaaaa tcataatttg ttagcaggag aaagtgtata ttatcatcta
     5101 gctttagttt ataaattaaa tcatcaaaaa gtaaatcatg acgcaattaa tgatatctta
     5161 gattttttag gtttgatgga tttaaaacag gtgaaatgcc atagtttgag tggtgggcag
     5221 caacaaaaag ttgcaatcgc tatggctgtt ttgcaaaaac cgaaacttat tctatgtgat
     5281 gagatcagct ctgccttaga tactaatagt gaaaaagaga tatttaactt gttgtctgat
     5341 cttcgtgaaa aatatggtat ctctatcttg atgattgctc atcacctatc tcttttaaaa
     5401 caatattgtg atcgtgtaat gattttggat caccagacta ttgtagatac tgttgttcca
     5461 gtgaaagcaa ctctgaatca attaaagtca aactacgtag atcaagtaaa ggagtttttg
     5521 ttacatgatt gacattattg aaaaacatgg cgaaggtatc ttaaaatcat tttgggaaac
     5581 caatttaatg ttagggttaa ccttgattct ttgttttcta attgcttttc caacaggtat
     5641 tttattgttt tcattgagaa agtcttactt gattaaacat tctttagcct atcaactact
     5701 aaatttattt ttaggtacgt tacggagtgt ccctttcctt atttttattt ttatcttaat
     5761 ccctctcaat cgattaattt ttgggacctc atttggaacg atagcggcaa tcttacctct
     5821 tactttggtt tcagtcagtt tgtatgcacg ttatgtggaa caagctttat taaatatacc
     5881 tcaagttgtt gtagatcgag ctctcagtct aggggctaac aagagacaga taatttatta
     5941 ttttttaatc ccctccatca agattgacct tgttttatca tttactgcaa cggctataag
     6001 tattcttggc tactcgacaa taatgggagt tattggtgca ggaggtttgg gtgaatatgc
     6061 ctatcgtttt ggctatcaag agtatgacta cccagttatg tacttgattg ttgtcttatt
     6121 tataatatat gtgtttatct tacagagttt gggctatttt atagcaaacc gctactcaag
     6181 aaaataaaag aaaagaggaa ctatgaaaaa attactttcc ctaacatgtc taatcatgat
     6241 gtctttatgt ttagtggcat gtactaagca agcaatgtcg tctaagcaaa ttaaagataa
     6301 gaatagtaaa gaaaaggtga ttactgttgc aacttacagc aaacctacat ctaccttttt
     6361 agatttgatt aaagataatg taaaagaaaa aggatatact ttaaaggttg tcatggtctc
     6421 tgactatatt caggctaaca ttgctttaga aaacaaagaa catgatgcta accttttaca
     6481 acatgaattt ttcatgagta tctttaataa ggaaaatgat ggtcatctag tgtcaattac
     6541 accaatttat cattcattgg ctggttttta tggtcaacat ttgaaaaata ttgccgagct
     6601 taaagacggt gctaaggtag cgattccgtc tgatcctgcc aatatgacta gagctctgct
     6661 attattgcaa gaaaagaaac ttatcacctt aaagaatacg tccaaaaaga ccaaggctat
     6721 cgaagatatt attactaacc ctaaaaaatt acgaattgaa cctgtagcat tacttaacct
     6781 caatcaggcc tattttgaat atgaccttgt ctttaatttc cctggatatg tgacaaaaat
     6841 caatctagtt cctaaaaggg atagattatt atatgagaaa aaaccagata tccgttttgc
     6901 aggtgccttg gtagctcgtg aagataataa aaa
//
LOCUS       DATROQ010000093         6879 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.094, whole genome shotgun sequence.
ACCESSION   DATROQ010000093 DATROQ010000000
VERSION     DATROQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.094"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..1080
                     /locus_tag="VBC94_001735"
     CDS             <1..1080
                     /locus_tag="VBC94_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO6979989.1"
                     /translation="FIFTKISFMFKPVFDFLAVLILPLVISGLLYYLLKPMVTFLEKR
                     GIKRVTAILSVFTIIILLLIWAMSSFIPMMSNQLRHFMEDLPSYVNKVQMETSSFIDH
                     NPWLKSYKGEISSMLSNISSQAVSYAEKFSKNVLDWAGNLASTVARVTVATIMAPFIL
                     FYLLRDSRNMKNGFLMVLPTKLRQPADRILREMNSQMSGYVQGQIIVAITVGVIFSIM
                     YSIIGLRYGVTLGIIAGVLNMVPYLGSFVAQIPVFILALVAGPVMVVKVAIVFVIEQT
                     LEGRFVSPLVLGNKLSIHPITIMFILLTSGAMFGVWGVFLSIPIYASIKVVVKELFDW
                     YKAVSGLYTIDVVTEERSEEVKNVE"
     gene            1070..2296
                     /locus_tag="VBC94_001736"
     CDS             1070..2296
                     /locus_tag="VBC94_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6979990.1"
                     /translation="MLNSEKMIVSIQNQDLEHANKYFEKALKNDPEEILLELGAYLES
                     IGFLPQAKRLYDQIRPNYPEVAINLAQIVAEDGDIEQAFLYLDYISEDSQEYVSALLV
                     MADLYDMEGLTDVAREKLLLASKLSDDPLVTFGLAEMNLSLEHYQEAIEGYASLDNRE
                     ILETTGVSTYQRIGKSYAIIGKFDAAIEFLEKAVDIEYDDLTVFELATILYDQEEYQK
                     ANLYFKQLDTINPDFDGYEYIYGLSLREEHKSEEALRLVQQGLRKNSFDGQLLLLASQ
                     LSYELHDVPSSESYLKQAEKVSENQDEIVMRLSNLYLEEERFEEVLELDNDDLENILA
                     KWNIAKAHKALEMDDSVDYYQSLYNDLKDNPEFLQDYAYILREFGYLDKAQEVGKAYL
                     KLVPDDIEMSEWVNNI"
     gene            2406..4088
                     /gene="alsS"
                     /locus_tag="VBC94_001737"
     CDS             2406..4088
                     /gene="alsS"
                     /locus_tag="VBC94_001737"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEO6979991.1"
                     /translation="MTESHNQYGADLIVDSLINHDVKYVFGIPGAKIDRVFDTLEDKG
                     PELIVARHEQNATFMAQAVGRITGEPGVVIATSGPGISNLATGLVTATDEGDAVLAIG
                     GQVKRGDLLKRAHQSMNNVAMLEPITKYSAEVHDPNTLSETVANAYRLAKSGKPGASF
                     ISIPQDVTDSPVSVKAIKPLSAPKLGSASVLDINYLAQAINNAVLPVLLLGNGASSEG
                     VTAAVRRLLDAVKLPVVETFQGAGIVSRELEDETFFGRVGLFRNQPGDMLLKRADLVI
                     AIGYDPIEYEARNWNAEISARIIVIDVEQAEIDTYFQPERELIGDMAHTLDLLLPAIK
                     GYELPEGSKEYLKGLRNNIENVSDVKFDRDSAHGLVHPLDLIDVLQDNTTDDMTVTVD
                     VGSHYIWMARYFKSYEARHLLFSNGMQTLGVALPWAISAALLRPNTKVISVSGDGGFL
                     FSAQELETAVRLHLPIVHIIWNDGKYNMVEFQEEMKYGRSSGVDFGPVDFVKYAESFG
                     AKGYRVDSKDSFEETLKQALIDAENGPVLIDVPIDYKDNVTLGETILPDEFY"
     gene            4102..4821
                     /gene="budA"
                     /locus_tag="VBC94_001738"
     CDS             4102..4821
                     /gene="budA"
                     /locus_tag="VBC94_001738"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEO6979992.1"
                     /translation="MSETVKLFQYSTLSSLMAGLYKGSLTIGELLTHGDLGIGTVHMI
                     DGELIVLDGKAYQAIGTDGKAEIIQLSDDVTVPYAAVLPHHIQKQFDINAETDNKDLE
                     EMILKNFEGQNLFKSLKIKGTFSRMHVRMIPKSPQHKRFADIASNQPEFTRENVSGTL
                     VGIWTPELFHGVGVKGFHVHFISDDLTFGGHVMDYSLTQGKVEIGKVDQLDQCFPTQD
                     QEFLKANFDLQKLREDIDLSE"
     gene            complement(4875..6530)
                     /locus_tag="VBC94_001739"
     CDS             complement(4875..6530)
                     /locus_tag="VBC94_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEO6979993.1"
                     /translation="MSFDGFFLHHLTNELQEQIEKGRIQKVNQPFDHELVLTIRNNRR
                     NYKLLLSAHPVFGRIQTTEANFQNPQNPNTFTMIMRKYLQGAVIETIQQIENDRILEI
                     IVSNKNEIGDHIKATLVVEIMGKHSNIILIDKNEHKIIESIKHVGFSQNSYRTILPGS
                     TYIAPPKTKAINPFDISDQTLFELLQTNDLSPKNLQQLFQGLGRDTALELSHCLKDNK
                     LNDFRQFFSREYYPSLTEKSFSAVQFSSSHETFQSLGQLLDYYYQEKAEKDRIAQQAS
                     DLIHRVQSELEKNIKKLAKQQDELLATENAEEFRQKGELLTTYLSMVPNNQDIVVLDN
                     YYTNQTIEISLDRALTPNQNAQRYFKKYQKLKEAVKHLKGIISDTKNTITYLESVETS
                     LNHASMEDINDIREELVETGFIKRRAHDKQHKRKKPEQYLASDGKTIIMVGRNNLQND
                     ELTFKMARKGELWFHAKDIPGSHVLIRDNLNPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLNKPSGTKPGFVTYTGQKTLRVTPTQEKIDSLKLKK"
BASE COUNT         2098 a         1092 c         1408 g         2281 t
ORIGIN      
        1 tttattttta ccaaaatctc ttttatgttt aaacctgtgt ttgattttct tgctgtgctg
       61 atattgccgc ttgtaatttc tggcttgctt tattacctat taaaacctat ggttacattt
      121 ttagagaagc ggggaattaa gcgtgtaaca gcgatattat cagtttttac tattataatc
      181 cttctgttaa tttgggcaat gtctagtttt attcccatga tgagtaatca attacgccat
      241 tttatggaag atctcccttc atatgtgaat aaagtgcaaa tggaaacaag ttcgtttata
      301 gatcacaacc cttggttaaa atcttataaa ggggaaatat cgagcatgtt atctaatatc
      361 agtagccaag cggtctctta tgctgaaaaa ttttcaaaga atgttttaga ttgggcagga
      421 aatttagcta gtacagttgc acgtgtgaca gtagcaacaa tcatggctcc ctttattttg
      481 ttttatcttt taagagatag tcgcaacatg aagaatggtt tcttaatggt tttaccaacc
      541 aaactacgcc aaccagctga tcgtattttg cgagaaatga atagtcaaat gtcaggatat
      601 gtgcaaggac aaatcattgt tgctattact gttggtgtta ttttttcaat aatgtatagt
      661 attataggcc ttagatatgg cgtgacatta gggattattg ccggtgtgtt aaatatggtt
      721 ccctatttgg gaagttttgt cgcccaaatt ccagtgttta tcttagcgct tgtcgcagga
      781 cctgttatgg ttgttaaagt tgcgattgtt tttgttattg agcaaactct agagggacgc
      841 tttgtctcac ctttggtttt aggtaataaa cttagcattc atccaattac aattatgttt
      901 attttattaa cctctggagc gatgtttggt gtttggggag tattcctcag tattccgatt
      961 tatgcatcta tcaaagttgt tgttaaagaa ttgtttgatt ggtacaaagc tgtcagtggg
     1021 ctatatacaa tagatgttgt tactgaagaa agaagtgaag aagttaaaaa tgttgaatag
     1081 tgaaaaaatg attgtttcta tccaaaatca ggatttagaa catgctaaca agtatttcga
     1141 aaaagcgttg aaaaatgatc ctgaggaaat cttattggaa ctaggagctt atttggagag
     1201 tattggtttt ttaccccaag caaaacgtct ctatgatcaa atccgaccaa attatccaga
     1261 agttgctatc aatttagctc aaattgttgc cgaagatgga gatattgaac aagcattctt
     1321 atacttagat tatatctcag aagatagtca agagtatgtt tcagccctcc tcgttatggc
     1381 tgacttatat gatatggaag gcttgactga tgttgcgcgt gaaaagcttt tgctagccag
     1441 caaacttagc gatgacccat tggttacatt tggtttagct gaaatgaacc ttagtttgga
     1501 acattatcaa gaagcgatag aaggatatgc atcacttgac aatcgagaaa tcttagaaac
     1561 gactggagtt tcaacttatc agcgtattgg taaatcctac gctattatag ggaaatttga
     1621 tgcagctatc gaatttttag aaaaagccgt tgacattgag tatgatgatt taacagtttt
     1681 tgagttagca acaatccttt atgatcaaga agaatatcaa aaagctaacc tttattttaa
     1741 acagttggat actattaatc cagactttga tggctatgaa tatatctatg gtttatctct
     1801 tcgtgaggag cataaatctg aagaagcttt acgtctagtt cagcaaggtc ttcgtaaaaa
     1861 tagttttgat ggtcaacttc tcttactagc ttcacaatta tcgtatgaat tacatgatgt
     1921 tccttcgtcg gagtcttatt tgaaacaagc tgaaaaagtt tctgaaaacc aagatgaaat
     1981 tgttatgaga ttgtcaaatt tgtatcttga agaagagcgg tttgaggaag tcttggaatt
     2041 agacaatgat gatttagaaa atatccttgc aaaatggaat atcgctaaag cacacaaggc
     2101 tttagaaatg gatgatagtg tagactatta tcaatctttg tataatgatt taaaagataa
     2161 tccagaattt ttacaagatt atgcttacat tttgagagaa tttggttacc ttgataaggc
     2221 acaagaagta ggaaaggctt atttaaaatt agttccagac gatatagaga tgtcagaatg
     2281 ggtgaataac atttaaatag gaaaacactt acagaaaaaa ataaaaaatt cacaagtaaa
     2341 ttttcacaat ctgatgtaaa aactgtgata taattgaacc attcttgtga aaagagggag
     2401 ataaaatgac agaatcacat aatcaatatg gggctgactt aattgtcgat agcctcatta
     2461 atcatgatgt caaatatgtt tttggtatcc caggagctaa gattgaccgc gtatttgaca
     2521 ctctagaaga taaaggtcca gagttaattg ttgctcgtca tgagcagaac gcgactttta
     2581 tggctcaggc tgtcgggcgt attacgggag aaccaggagt tgttattgca actagtggac
     2641 ctgggatctc gaacttggca acagggttag ttaccgcgac agatgaaggt gatgctgttc
     2701 ttgcaattgg tggacaggtt aagcgaggag atttattgaa acgtgctcat caatcaatga
     2761 ataacgttgc tatgctagaa cctattacaa aatattctgc tgaggttcat gaccctaata
     2821 ctttgtcaga aactgttgca aacgcctatc gtcttgctaa atctggtaaa ccaggagcaa
     2881 gttttatctc tattcctcaa gacgttacag acagtccagt ctcagtaaaa gcaatcaaac
     2941 ctcttagtgc acctaaattg ggttcggcat ctgttttaga tattaattat ttagcgcaag
     3001 ctattaacaa tgctgtttta ccggtactgt tattaggaaa tggcgcatct tcagagggag
     3061 taactgctgc cgtacgtcga cttcttgatg ctgttaaact accagtagta gaaactttcc
     3121 aaggtgcggg tattgtgtca cgtgagctcg aggatgaaac attctttggt cgtgtaggcc
     3181 tcttccgtaa tcaacctgga gatatgcttt tgaagcgtgc agacctagtt attgctatcg
     3241 ggtatgaccc tattgaatat gaggcgcgta actggaatgc tgaaatttct gctcgtatta
     3301 tcgttattga cgttgaacaa gcagaaattg atacgtattt ccaacccgag cgagaactga
     3361 ttggggacat ggcacataca cttgatttac ttttaccagc tattaaagga tatgaattgc
     3421 cagaaggttc aaaagagtat ttgaaaggtc ttcgtaataa cattgaaaac gttagcgatg
     3481 ttaaatttga tcgtgactca gctcatggac ttgttcatcc tcttgatctc attgatgtac
     3541 tccaagataa taccaccgat gacatgactg ttacagttga tgtgggaagc cattatattt
     3601 ggatggctcg ctactttaaa tcatatgaag cgcgtcactt gctcttctca aacggaatgc
     3661 aaacattagg agttgctcta ccttgggcaa tttctgcggc tcttttgcgt cccaacacaa
     3721 aagttatttc agtttctggt gatggtggct tcttattctc agctcaagag ttagaaactg
     3781 cagttcgtct tcatctacca attgttcaca ttatttggaa cgatggaaaa tataacatgg
     3841 ttgaattcca ggaagaaatg aagtatgggc gttcttcagg tgttgacttt ggaccagttg
     3901 attttgtaaa atacgctgaa agtttcggtg ctaaaggtta ccgtgtagac agcaaagaca
     3961 gttttgaaga aacactcaaa caagctctaa tcgatgctga aaatggacca gtgttgattg
     4021 atgtgcctat tgactataaa gataacgtga cactaggtga aacaatctta cctgacgagt
     4081 tctactaaga cgaggtctaa tatgtcagaa acagttaaat tatttcaata cagcaccttg
     4141 agttcactga tggcaggatt gtacaaaggc agtctaacta tcggagaatt attaactcac
     4201 ggtgacttag gaatcggtac tgttcatatg attgatggtg aattgattgt attggatggg
     4261 aaagcttatc aagctattgg tacagatggt aaagcagaga ttatccaatt atcagatgat
     4321 gtgacagtac cctatgcagc ggttttacca caccatatcc aaaaacaatt tgatatcaat
     4381 gctgagacag ataataagga tttggaagag atgattctaa aaaactttga aggtcaaaat
     4441 ctctttaagt ctttaaaaat caaaggaacg ttttcaagaa tgcacgttcg tatgattcca
     4501 aaatctccac aacataaacg ttttgcagat attgcatcta atcagccaga atttaccaga
     4561 gagaatgtat ctggtacgtt ggttgggatt tggacgccgg aattatttca tggtgtgggg
     4621 gtaaaagggt tccatgtcca ctttatttca gacgatttaa ctttcggtgg gcatgttatg
     4681 gattactcgc tcacacaagg aaaagttgaa attggaaagg ttgaccaact tgaccaatgt
     4741 ttcccaactc aagatcaaga atttttgaaa gctaactttg acctacaaaa gcttagagaa
     4801 gatattgatt taagtgaata agaataccta taaataatta ataacactaa aaatagacag
     4861 ggatatgtcc cagtctattt ttttagttta aggctatcta ttttttcttg tgtgggagtg
     4921 actcgtaagg ttttttgacc agtgtaagtt acaaaacctg gcttagtacc acttggttta
     4981 ttgagttttt ttgcttcaat catgtctact tgaacaagat tggagagacg agctttagag
     5041 taataagcag ctaactcggc ggcatctgtc ttaacctcgt cacttggatt gaggttatcc
     5101 cgaattaaca catggcttcc ggggatatcc ttggcatgaa accacaactc ccctttacga
     5161 gccattttaa atgttaactc gtcattttgg agattattac gtcccaccat aataatagtc
     5221 ttgccatcgg atgctaaata ttgttcaggt tttttacgct tatgttgttt atcatgtgcg
     5281 cgtcgcttaa taaatccagt ttcaacaagt tcttcacgaa tatcattgat atcttccata
     5341 gaagcatgat ttagcgatgt ttcaacagat tcaaggtagg tgattgtatt tttcgtatct
     5401 gaaataattc cttttaaatg ttttacagct tcttttaatt tttgatattt tttaaagtag
     5461 cgttgggcat tttggttggg tgttaaagct cgatcaagtg aaatttcaat cgtttggttg
     5521 gtgtaataat tatcaagaac aacgatatct tgattatttg gcaccataga gagataggta
     5581 gttagaagct ctcctttttg gcgaaactcc tctgcatttt cagtagctag aagttcgtct
     5641 tgttgcttag ctagtttttt gatatttttc tctaactcac tttgaacacg gtgaatgagg
     5701 tcactagctt gctgtgctat gcgatctttt tcagccttct cttggtagta ataatctaac
     5761 aattgtccaa gagactgaaa tgtttcgtga ctgcttgaga attggacagc ggaaaacgat
     5821 ttttctgtta ggctaggata atattctctc gaaaagaatt gacgaaaatc gttaagttta
     5881 ttgtctttca aacagtgaga taattctagc gcagtatcgc gtcctaaacc ttggaagagc
     5941 tgttggaggt ttttagggct taggtcattg gtttgaagca gctcaaatag agtttgatca
     6001 gaaatatcaa aaggattgat tgcttttgtt ttgggtgggg caatgtaagt tgaaccagga
     6061 agaatagtac ggtaggagtt ttgcgagaaa ccaacatgct taattgattc aataatttta
     6121 tgttcatttt tatcaatcaa gataatgtta ctgtgttttc ccatgatttc aacaacaagt
     6181 gtcgctttaa tgtgatcacc gatttcgttt ttattagaca cgatgatttc taatatacgg
     6241 tcattttcaa tttgttgaat ggtttcaatc actgcacctt gcaaatactt tctcatgatc
     6301 attgtaaaag tatttggatt ttgaggattt tggaaatttg cttcggtagt ttggatacgt
     6361 ccaaatacag ggtgagcaga taggagtagt ttatagttgc gacgattatt gcgtattgtt
     6421 aataccaatt catggtcaaa aggttggtta actttttgga tacgtccttt ttcaatctgt
     6481 tcttgtaatt catttgtgag gtgatgtaaa aaaaatccat caaaagacat ggcgtccccc
     6541 ttattctaaa aattatagct tatttattat accataggct gagctaaaaa tattttttta
     6601 taacgcaaaa ggcttgcaat tttcagaata tagtatataa ttatagaaaa attcatgaga
     6661 aaggatgact agtaaatgac taaaacatta tttaccaatt ggcaaaactg gcgtagataa
     6721 agttgtgttt atgggtgcat agatacgact ttttggagta cttcaaaaaa gtatctatgc
     6781 gctggtctag tctaacaata gagagcgcaa ggtaagttat ttatcaggcg ctttttcttt
     6841 tgtgctaaac tccagaaagt caggatatta ttaaagtga
//
LOCUS       DATROQ010000094         6653 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.095, whole genome shotgun sequence.
ACCESSION   DATROQ010000094 DATROQ010000000
VERSION     DATROQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.095"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..1825
                     /locus_tag="VBC94_001740"
     CDS             <1..1825
                     /locus_tag="VBC94_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEO6979994.1"
                     /translation="AYQEMKASVNLMMRNTFVFNDNWDMEPCSKAYCLDPLEWDNPVT
                     DDPEWLYMLNRQTYLFKFLVVYIVEGDKSYLRQMKYFMYHWIDCQFTLKPEGAVSRTI
                     DTGIRCMSWLKVLIFLDYFGLITETKKIKLLTSLREQITYMRDYYREKDSLSNWGILQ
                     TTAILACLYYYEDELNLPEIQSFAEEELLLQIKLQILDDGSQYEQSIMYHVEVLKSLM
                     ELVILAPKYYLPLEETIEKMVTYLIAMTGPDYCQLAIGDSDVTDTRDILTLATLVLKS
                     SKTKSFSFDNVNLETLLLFGKPSIYLFEEIPRATIGESAYLFPDSGHVCLRDDRRYIF
                     FKNGPFGSAHTHSDNNSVCLYDKKKPIFIDAGRYTYKEEQLRYDFKRSTSHSTCTLDG
                     QSLEMIKDSWTYNSYPKCDYCQLTSKDRYHLVEGQLHVQRASDIYYHKRWLLTLPQAI
                     TLVIDKVNCPGEHVLTNQYILDDQVIYENGLVNDLKLVSPTTFNLEDCLISKRYNQLT
                     ESHKLVKKIKFVDEVMDYTLIVDRNCQVKCVPLVQTNSHKELSNSIAFDIRSQDFHYL
                     IGVHMDDIIFGDKLYLMQGIKCKGKVIVYDKNNGKMSRLKN"
     gene            1905..2906
                     /locus_tag="VBC94_001741"
     CDS             1905..2906
                     /locus_tag="VBC94_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6979995.1"
                     /translation="MSKKMTINDIAQLSKTSKTTVSFFLNQKFEKMSDETRQRIQEVI
                     DETGYRPSTIARSLNSKKTKLLGVLIGDITNTFSNQIVKGIEHITKQKGYQIIVGNSN
                     YDAKSEEDYIENMLNLGVDGFIIQPTSNFRKYSRILKEKKKPMVFFDSQLYEHKTSWV
                     KANNYDAVYDMTQECLNRGYKKFIMITADTSLLSTRIERASGFMDALKDNGFGYDTLV
                     IEDDDHSKSDIEDFLKAVVPDKEETLVFAPNCWALPMVFTAMKNLNFDMPRVGLVGFD
                     NIEWTDFSSPKVSTIVQPAYEEGEQVAQILINRIEGDDSVDNQQIVDCQMFWKESTF"
     gene            complement(2978..3592)
                     /locus_tag="VBC94_001742"
     CDS             complement(2978..3592)
                     /locus_tag="VBC94_001742"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO6979996.1"
                     /translation="MSAIDKLVKASHLIDMNDIIREGNPTLRKVAEEVTFPLSEKEEI
                     LGEKMMQFLKHSQDPIMAEKLGLRGGVGLAAPQLDISKRIIAVLVPNVEDAQGNPPKE
                     AYSLQEVMYNPKVVSHSVQDAALSDGEGCLSVDREVPGYVVRHARVTIEYFDKTGEKH
                     RLKLKGYNSIVVQHEIDHIDGIMFYDRINEKNPFAVKEGLLILE"
     gene            complement(3658..4302)
                     /locus_tag="VBC94_001743"
     CDS             complement(3658..4302)
                     /locus_tag="VBC94_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEO6979997.1"
                     /translation="MDIENYCQDYCLETIFLKHYFKDLQVITLTAGQSVCKQGEQLEY
                     LHYIVKGRFKIVRRLFNGKEHILDIKTKPTLIGDIELLTNRQIVSSVIALEDITVIQL
                     SLKGRKEKLLTDATFLLKLSQELAQAFHDQNIKASTNLGYTVKELLASHILAIEEQGC
                     FHLELSSLADSFGISYRHLLRVIHDMVKEGLIQKEKPKYFIKNRFALESLNIQP"
     gene            4414..5577
                     /locus_tag="VBC94_001744"
     CDS             4414..5577
                     /locus_tag="VBC94_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6979998.1"
                     /translation="MKIFMEKLSLLSLSLILLSTFSTSPALPQMISYYRDKGLPSPQV
                     ELLFSIPSMAIIFILLITPWLSKKLSEKHMIIFGLLLTALGGGLPVVSQNYLLVFVSR
                     LLLGSGIGFINARAISVISEYYQGKERRKLLGLRGSFEVLGNAGLTALVGLLLTFGWS
                     KSFMIYFLALPILVLYLVFAPKKVVKDTNDKIKTKGQKIPKADLTYIVALAILAGFVI
                     TINTGINLRIPLLVVEFGLGTPAQASLVLSAMMLMGIIAGMSFGQLIAMFHKQLIPIC
                     LVLFSLTLLGVGLPSNLMVLTISAMASGFLYSLMVTAVFSLVADRVEYSLVGSATTLV
                     LVFCNIGGASAAILLSCFDHLLGQINAVFYVYAILSLAVGMIYFLKRLFFKKM"
     gene            complement(5623..6606)
                     /locus_tag="VBC94_001745"
     CDS             complement(5623..6606)
                     /locus_tag="VBC94_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6979999.1"
                     /translation="MTEKYYNWATLGTGVIANELAQALEARGQKLYSVANRTYDKGLE
                     FANKYGIQKVYDHIDQVFEDPEVDIIYISTPHNTHISFLRKALANGKHVLCEKSITLN
                     STELKEAIDLAETNHVVLAEAMTIFHMPIYRQLKTLVDSGKLGPLKMIQMNFGSYKEY
                     DMTNRFFSRDLAGGALLDIGVYALSCIRWFMSEAPHNITSQVTFAPTGVDEQVGILLT
                     NPANEMATVSLSLHAKQPKRATIAYDKGYIELFEYPRGQKAVITYTEDGHQDIIEAGK
                     TENALQYEVADMEEAISGKTNHMYLNYTKDVMDIMTQLRQEWGFTYPEEEK"
BASE COUNT         2075 a         1035 c         1264 g         2279 t
ORIGIN      
        1 tgcctatcaa gaaatgaaag cctctgttaa tttgatgatg aggaacactt ttgtatttaa
       61 tgataattgg gatatggaac cttgttctaa agcatattgt cttgatcctt tggagtggga
      121 taatccagta acagatgatc cagaatggtt gtatatgcta aatcgtcaaa cttatctttt
      181 taaattcctg gtggtctaca ttgttgaagg cgataagtct tatcttagac aaatgaaata
      241 ttttatgtac cattggattg attgccaatt tacactaaag ccagaagggg cggtaagtcg
      301 tactattgat actgggatac gttgcatgtc atggctcaaa gttttgattt ttttagatta
      361 ttttggatta ataacagaaa ctaaaaaaat taaactatta actagtttac gggagcagat
      421 aacttatatg agggactatt atcgtgaaaa agatagtcta agtaactggg gaattttaca
      481 aacaacagca atattggcgt gtttatacta ttatgaagat gaattaaacc tacctgaaat
      541 tcagagtttt gctgaagaag aattattact tcaaatcaag cttcaaattt tagatgacgg
      601 gagccagtat gaacaatcaa ttatgtatca tgtagaagtc ttgaagtcct taatggaact
      661 agttatcctt gcgcctaaat attatctacc attagaagaa actattgaaa aaatggttac
      721 ctatctaatt gctatgactg gtccggatta ttgtcaacta gctatagggg acagtgatgt
      781 gaccgatact cgtgatattc taactttggc aacacttgtg ttgaaatctt ctaaaacaaa
      841 atcattttct tttgataatg ttaatttaga aactttactt ttgtttggga agccatcaat
      901 ttatcttttt gaagaaatac cgcgtgcaac aataggtgag tctgcttatc tttttccaga
      961 ttctggtcat gtgtgtttac gtgatgatag gcgttatata ttttttaaga acggtccatt
     1021 tggtagcgct catactcata gtgataataa tagtgtttgt ctctatgata aaaagaaacc
     1081 tattttcatt gatgcaggaa gatatactta caaagaagaa caactaaggt atgattttaa
     1141 acgttcgact agtcattcaa catgtaccct tgatgggcaa tctttagaaa tgatcaagga
     1201 ctcttggaca tacaattctt atccaaaatg tgactattgt cagctgactt caaaggatag
     1261 gtaccattta gtcgaaggac aactacatgt ccaaagagct tctgatatct attaccataa
     1321 gcgatggttg ttaactttac cgcaggccat taccttagtt attgataagg tgaattgtcc
     1381 aggagagcat gttttaacaa atcaatatat tttagatgat caggtcattt atgaaaatgg
     1441 gcttgttaac gacttgaaat tagtaagtcc tacgaccttt aatctagaag attgccttat
     1501 ttctaagcgg tataatcaat tgacagaaag tcataaatta gttaagaaaa taaaatttgt
     1561 tgatgaggtg atggattata ccttgatagt tgatcggaac tgtcaggtga aatgtgttcc
     1621 tttagtgcaa acgaacagtc ataaggaact aagtaatagc attgcatttg atattaggtc
     1681 tcaagacttt cattatttaa ttggagtgca tatggatgat attatctttg gggataaact
     1741 ctatttgatg caagggataa aatgcaaagg aaaagtcatt gtatatgata aaaataatgg
     1801 caaaatgagt cgtttaaaaa attaagaggg ctattattat gctatactag tactatatta
     1861 accataaaaa aataaaatca gtaaaattag aaaggaggtt tgttatgtct aaaaaaatga
     1921 ccattaatga catcgctcaa ttatcaaaaa cttccaaaac aacggtttct tttttcttga
     1981 atcaaaaatt tgaaaaaatg tcggatgaga cgaggcagcg tattcaagag gtgattgatg
     2041 aaacaggtta tagaccaagt acgatagcaa gaagtctaaa ttctaaaaaa acaaaactac
     2101 taggtgtctt aattggagat ataacaaaca cattttcaaa tcaaattgta aaaggaattg
     2161 agcacatcac caaacaaaaa ggctatcaaa ttattgtggg taatagtaat tatgatgcca
     2221 aaagtgaaga agattatatt gaaaatatgt taaatttagg tgttgatggt ttcattattc
     2281 agcctacatc aaattttcga aagtattctc gtattttaaa agaaaaaaag aaaccaatgg
     2341 tattttttga tagtcaactt tatgaacata agacgagttg ggttaaggct aataactatg
     2401 atgctgttta tgatatgaca caggagtgtc taaatcgagg gtataaaaag tttattatga
     2461 ttactgctga tacgagcttg cttagtacac gtattgagag agcttcggga ttcatggatg
     2521 ctttaaagga caatggtttc ggttatgaca ctttggtgat tgaagatgat gatcattcaa
     2581 aaagtgatat agaagacttt ctaaaagcgg tagttccaga taaggaggag acattagtat
     2641 ttgcaccaaa ttgttgggct ttaccaatgg tttttactgc catgaaaaat ctcaattttg
     2701 atatgccacg agttggttta gttgggtttg acaatattga atggacagac ttttcatctc
     2761 ctaaagtgtc aacaattgtc cagccggctt atgaagaagg tgaacaagta gcacagattt
     2821 tgattaatcg tattgaagga gatgactccg tcgataacca acagattgta gattgccaga
     2881 tgttttggaa agagtcgact ttttaaaaaa agatttctta ttttatttgc tattaaaaaa
     2941 taaaacctag agaaaattat ctctaggtta tttgtcctta ttctaaaata agtaagcctt
     3001 ctttgacagc gaaaggattt ttctcattga tacggtcgta aaacataata ccatcaatgt
     3061 ggtcaatttc atgttgaaca acaatagagt tgtaaccttt taatttgagt cgatgttttt
     3121 caccagtttt gtcgaaatat tcaatagtta cacgcgcgtg gcggacgaca tagccaggta
     3181 cttcacgatc tactgatagg catccctccc cgtcagataa agcagcatct tgaacagaat
     3241 gcgatacaac tttagggttg tacattactt cttggaggct ataagcttct ttaggtggat
     3301 ttccttgagc atcctcaacg tttggaacaa ggacggcaat gatgcgtttt gagatgtcta
     3361 attgtggtgc ggctaaacca acaccaccac gtaagcctag cttctcagcc ataattggat
     3421 cttgagagtg ttttaaaaat tgcatcattt tttctccgag aatttcttct ttctcagaaa
     3481 gtgggaatgt gacttcttca gccactttgc gaagggttgg attgccttcg cggataatgt
     3541 cattcatgtc aattaaatga ctagctttta ctaatttatc aatagcagac atattttctc
     3601 ctaactgtat tctctgattt agtttagcat aaaataatta aatgataaag ctacgcttca
     3661 tggctgaata tttaatgatt ctagtgcgaa acgatttttg ataaagtatt ttggtttttc
     3721 tttttggata aggccttctt tgaccatatc atggataact cttaataaat gacgataact
     3781 gatgccaaac gaatcagcta aactacttag ttcaaggtga aagcaacctt gttcttcaat
     3841 tgctaaaata tgacttgcaa gtaactcttt gacggtatac cctaaattag tagacgcttt
     3901 gatgttttga tcatgaaaag cctgagctag ctcttgacta agttttagga ggaaagttgc
     3961 gtctgttaac agtttctctt tccgtccttt aagtgataat tgaatgactg taatatcctc
     4021 taaggcaata acagatgata caatttgtct attagtcaaa agttcaatat caccaatcag
     4081 tgttggtttt gttttaatat ctaaaatatg ttctttgccg ttaaagagtc gacgtacaat
     4141 tttaaaacgt cccttgacga tgtagtgcaa gtattctaat tgttctccct gtttacagac
     4201 ggattggccg gcagttaggg tgattacttg taaatccttg aaataatgtt ttagaaagat
     4261 agtctccaaa cagtagtctt ggcagtagtt ttctatatcc atgtattgac tccctatttg
     4321 agatagtttt aggacatatg tcatatattt ctagtttaat attgattata ctttatttat
     4381 ctaaaaaaag acatcagaaa gaggtattgt cacttgaaaa tctttatgga gaagctaagt
     4441 ttactctctt tatcacttat cttgctatca actttttcga catcaccagc cctaccacaa
     4501 atgatatctt attatagaga taagggctta ccatcacccc aagttgaatt attattttca
     4561 attccttcga tggctattat cttcatcttg cttatcacac cttggttaag taaaaaactt
     4621 tctgagaaac atatgattat ctttggacta ttgctaacag cattaggggg aggattgccg
     4681 gttgtcagtc agaactacct tttagtattt gtatcgcgtc ttttattggg gagtggtatc
     4741 ggttttatta atgctagggc cattagtgtg attagtgaat attatcaagg caaagaaaga
     4801 agaaaactct taggattaag ggggtccttt gaagttttag gaaatgctgg tttgacagcc
     4861 ttagttgggc tattgttgac ttttggttgg tctaaatcat ttatgattta ttttttagct
     4921 ttaccaatct tagttctata tcttgttttt gcgcctaaga aagtggtgaa agatacaaat
     4981 gacaagatta aaactaaagg tcaaaaaatc cctaaagctg atttgactta tattgtcgcc
     5041 ttagctattt tagcaggatt tgtcataact ataaatactg ggattaatct taggatacct
     5101 ctgctagtag tagaatttgg tttgggtacg ccagctcaag ctagtcttgt tcttagtgcg
     5161 atgatgctta tgggaataat agcaggtatg agttttggtc agttgatagc tatgtttcat
     5221 aaacaattaa ttccaatctg cctcgtctta ttttctttaa cattgcttgg agttggattg
     5281 ccttctaatc taatggtact aacaattagt gcgatggcgt caggtttcct ttacagcctg
     5341 atggtcactg ctgtgtttag cctagtagca gaccgtgttg aatattcgtt ggttggttct
     5401 gcaacgacat tagttttagt attttgtaat attggtggag ctagtgcggc tattcttctg
     5461 tcttgttttg atcatttact aggacaaata aatgcagttt tctatgttta tgctatttta
     5521 agtttagcag ttggtatgat ttatttcctt aaacgcctat tttttaagaa aatgtagtgt
     5581 atattaacta aaaagggctt gacaatcatt gtcaagctct cttcattttt cttcttctgg
     5641 gtaggtaaat ccccattctt gacgtagctg tgtcatgata tccataacat ctttggtata
     5701 gtttaagtac atgtggttag tttttcctga aatggcttct tccatatcag ctacctcata
     5761 ttggagagca ttttcagttt tgccagcttc gataatatct tgatgcccat cctcagtata
     5821 agtaataact gccttttgtc ctcgcggata ttcaaaaagt tcaatgtagc ctttatcgta
     5881 agcgatagtt gctcgtttag gttgttttgc atgtaaacta aggctgacag tcgccatctc
     5941 atttgctggg ttggttagta ggataccaac ttgttcatca acccctgttg gtgcaaatgt
     6001 aacttgagag gtaatgttgt gaggtgcttc tgacataaac cagcgaatac aagaaagtgc
     6061 ataaacacca atgtccagca aagcaccgcc tgctaggtca cgactgaaaa aacggttagt
     6121 catatcatat tctttataac ttccgaaatt catttgaatc atttttaacg gtcctaattt
     6181 tccactatca actaatgttt ttaattggcg gtaaattggc atatgaaaaa tagtcatggc
     6241 ttcagctaag acaacatggt tagtttcggc taaatctatg gcttctttaa gctcagtact
     6301 atttaaagta atagattttt cgcaaagaac gtgcttacca tttgctaaag cctttcgtaa
     6361 aaatgagatg tgagtattgt ggggagtaga gatataaatg atatccactt cagggtcttc
     6421 aaatacttga tctatgtgat cataaacttt ttggatacca tatttgttag caaattcaag
     6481 tcctttgtcg taagttctat tagctacaga atataatttt tgtccacgtg cttccagtgc
     6541 ttgggctaat tcgttggcaa taacgccggt tccaagcgtt gcccaattat aatatttttc
     6601 agtcataaca acctccttgt tggtttatag tatagcaaaa ataacaaaat gat
//
LOCUS       DATROQ010000095         6544 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.096, whole genome shotgun sequence.
ACCESSION   DATROQ010000095 DATROQ010000000
VERSION     DATROQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.096"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            59..289
                     /locus_tag="VBC94_001746"
     CDS             59..289
                     /locus_tag="VBC94_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980000.1"
                     /translation="MAFEPKNSELTKVLKESLDEEKKEIFSSEMNIRDFERTKQYQFT
                     LQPSVRKKIDRLSKEKGYRSASSFINDFFKNL"
     gene            386..935
                     /locus_tag="VBC94_001747"
                     /pseudo
     CDS             386..935
                     /locus_tag="VBC94_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584204.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1043..2377
                     /locus_tag="VBC94_001748"
     CDS             1043..2377
                     /locus_tag="VBC94_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
                     /protein_id="HEO6980001.1"
                     /translation="MEVKKFSEKDFVNEINKIKQKQFLSQIEQYESYIAPQMRTKGYK
                     RINQSERTVVFSFGEITFSRSRWTNGFETRIPVDEWLGLEKYKRYSIEFLYHVAKLAT
                     MMPYRQVCKVIDSTLQTIITKDCVLKAVKFVEKLLKEKERYRFYLEEPPERKKVKKLY
                     VEGDGVMIKSTDSREERRYLDLTHFVIHTGSKKVSTKRYELRDKHEILQLNYDKAKYN
                     LLDYIYNNYEVDDDTILITNSDMGKGYTSRVFKELGKALKVKKHEHFWDIYHVKEKLS
                     SYLRKYPIELTDFALDAVKKYNSDKLELVFDTVESLICDELEDQEFQKFKKKVLNNFK
                     YIKPAHLRNLSNRGIGIMESQHRKITYRMKRRGMYWSKWGISTMANMIILERANGLRE
                     LFFGSWRKVYSEYKEGSFSAGRLFKKTDELDKFSKPLLKNGRKWSITGIKTK"
     gene            3031..6483
                     /gene="scpA/B"
                     /locus_tag="VBC94_001749"
     CDS             3031..6483
                     /gene="scpA/B"
                     /locus_tag="VBC94_001749"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HEO6980002.1"
                     /translation="MRKKQKLPFDKLAIALISTSILLNAQSDIKANTVTEDTPATEQA
                     VEPPQPIAVSEESRSSKETKTSQTPSDVGETVADDANDLAPQAPAKTADTPATSKATI
                     RDLNDPSHVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKENLEKAK
                     KEHGITYGEWVNDKVAYYHDYSKDGKNAVDQEHGTHVSGILSGNAPSEMKEPYRLEGA
                     MPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDETK
                     KAFDYAKSKGVSIVTSAGNDSSFGGKPRLPLADHPDYGVVGTPAAADSTLTVASYSPD
                     KQLTETATVKTDDHQDKEMPVISTNRFEPNKAYDYAYANRGTKEDDFKDVEGKIALIE
                     RGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRRDGLLLKDNP
                     PKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAKL
                     SGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYFS
                     PRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELYY
                     QVTVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMKN
                     GYFLEGFVRFKQDPTKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSDA
                     KDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGTF
                     AKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGNV
                     VWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPISS
                     GAKEQHTDFDVIVDNTTPEVATSATFSTEDSRLTLASKPKTSQPVYRERIAYTYMDED
                     LPTTEYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLEG
                     HSNKPEQDGSDQAPDKKPEAKPEQDGSGQTPDKKKETKPEKDSSGQTPGKTPQKGQSS
                     RTLEKRSSKRALATKASTRDQLPTTNDKDTNRLHLLKLVMTTFFLGLAAHIFKTKRQK
                     ETKK"
BASE COUNT         2403 a         1114 c         1249 g         1778 t
ORIGIN      
        1 aaatataaac atagttatat acataaatat gactatgttt ataagaaaga ggatgaatat
       61 ggcatttgaa ccaaaaaatt cggaactaac aaaagttcta aaagaaagtt tagatgaaga
      121 aaagaaagaa attttctcat ctgaaatgaa tattagggac tttgaacgta caaaacaata
      181 tcaatttaca cttcaacctt cagtccgaaa aaaaattgat aggctatcaa aagaaaaagg
      241 ctatcgttca gcatcatctt ttataaatga cttttttaaa aatttgtaaa caaagtttga
      301 attatattta ttatggaaat cattattaga tgggaaaaat ggttaagaaa aaagtggttg
      361 tggtggatgt ttaagtttaa taatagtgat aattattgtt agtagtgtta taaatttatt
      421 tacaggttct gaaagcagta atacttctaa aaagaataag acaaatccta gtggtgcgac
      481 aagtgcaaaa gtcgttaaac acaaaaaaac tgatgaagaa cgagcagaag attatataaa
      541 aacgttagga ttaaacccga gtgattatat aaaagaaatg gattattcaa aggttgattt
      601 taggggggga tttatcatca gtatcaggta attataataa ttttactgtt gaaaacatca
      661 atcaaacttt tgaaaaaggt gataaatctt taaaagatat tattgaaatt tttggaaagc
      721 ctgatacaat ttgggttaat tacgttgaca atcagtacaa tactgatttg tactggaaaa
      781 aagatgacac agatgattct aattatattc atgcagaatg gtctgagcca aaagatgaat
      841 atgatgttca aagtttaagt ggcacggctt caaattcagg tactggcaag tttcagtcat
      901 tcattggatt agatggattt gagcctgcac agtaggataa atagaaagat cacgttatta
      961 aatgtggtct tttactttaa cttttcgaat aaaaaatagc aataatattc taatcctgta
     1021 acaaaaattg taaaatatta tcatggaagt taaaaaattc tcggaaaaag attttgtaaa
     1081 tgaaataaat aaaataaaac agaaacaatt tttaagtcaa attgaacagt atgaaagcta
     1141 tatcgctcct caaatgagaa cgaaaggcta taagaggatc aatcagtctg aacgtacggt
     1201 tgttttctct tttggagaaa taacatttag taggagtcgc tggacaaatg gctttgaaac
     1261 tagaatacca gtagatgagt ggttaggtct tgaaaaatat aagagatatt caatagaatt
     1321 cttatatcat gttgcaaaat tggctacaat gatgccttat cgtcaagttt gcaaagtaat
     1381 agatagcact ttgcaaacaa tcataacaaa agactgtgtt ttaaaagcag taaaatttgt
     1441 agaaaaattg ttaaaagaaa aagaacgcta tcgtttttat ttggaagagc cacccgaacg
     1501 taaaaaagtg aaaaaactgt atgttgaggg tgatggagtc atgattaaaa gcacagattc
     1561 tagagaggaa agaaggtatt tagatttaac acattttgtt attcatacag gctcaaaaaa
     1621 agtttctact aaaagatatg aattgcggga caagcacgaa atattacagc ttaattatga
     1681 taaagctaaa tataatcttt tagattatat ttataataac tatgaagtag atgacgatac
     1741 tattttaatc actaactctg atatgggtaa aggctatact agtagagttt ttaaggaatt
     1801 aggaaaagca cttaaggtaa agaaacatga gcatttttgg gatatctatc atgttaaaga
     1861 aaagttaagt tcatacctta gaaaatatcc aattgaatta accgattttg ctttagatgc
     1921 ggtaaaaaaa tataattctg ataagcttga attagttttt gatactgttg aatcactgat
     1981 ttgtgatgaa cttgaagatc aagaatttca gaagtttaag aaaaaagtat taaataattt
     2041 caaatatata aaaccagctc atcttagaaa tctttcaaat cgtggtattg gtatcatgga
     2101 atcacaacac agaaagataa cgtatagaat gaagcgacgt ggcatgtatt ggtcaaagtg
     2161 gggaatctcc acaatggcaa atatgattat acttgaaaga gctaacggtt tacgagaatt
     2221 atttttcggt tcttggagaa aggtatacag tgagtataaa gaaggttcat ttagtgcagg
     2281 gcgacttttt aaaaagacag atgaattaga taaattttct aagccccttc taaaaaatgg
     2341 cagaaaatgg agtataacag gaatcaaaac aaaatagaaa aagtctaaag atacctttat
     2401 ttctagcgtt tttttgcatg ttttaacttg cattaaatct tctaaagtag tataattata
     2461 ttaaagaaaa gcaaaaaagg tgaaggagcg cgaactccta caccaaaccg attaagacaa
     2521 ataccacttg cttaatcagt cgaaccttat tgtagcataa ctaaaacaat accgtcaaga
     2581 ctgaacaagc aaaaaatact tgttttggtc tttttaagaa taattcaagt tttcttaaat
     2641 ttatgaggta tcgactaatc tcaaaatttc agacacatac ctgagtgaac atcaagagag
     2701 aaccgatcgg ttctctcttg atgtctaaaa gaatgaggtt aaggaggtca caaactaaac
     2761 aactcttaaa aagatgacct ttactaataa tcgtctttgt tttataataa agatgttagt
     2821 aatataattg ataaatgaga tacatttaat cattatggca aaagcaagaa aaatagctgt
     2881 gtcatatgca aataacccct gtttgctctt taaaaaagac gttatcctta tttctctaca
     2941 cacagacgga tagctaggag agaatcgttt gattctctct tttcttaatg gtcataaaga
     3001 caaagtctct catcatgaaa ggacgacaca ttgcgtaaaa aacaaaaact accatttgat
     3061 aaacttgcca ttgcgcttat atctacgagc atcttgctca atgcacaatc agacattaaa
     3121 gcaaatactg tgacagaaga cactcctgct accgaacaag ccgtagaacc cccacaacca
     3181 atagcagttt ctgaggaatc acgatcatca aaggaaacta aaacctcaca aactcctagt
     3241 gatgtaggag aaacagtagc agatgacgct aatgatctag cccctcaagc tcctgctaaa
     3301 actgctgata caccagcaac ctcaaaagcg actattaggg atttgaacga cccttctcat
     3361 gtcaaaaccc tgcaggaaaa agcaggcaag ggagctggga ccgttgttgc agtgattgat
     3421 gctggttttg ataaaaatca tgaagcgtgg cgcttaacag acaaaactaa agcacgttac
     3481 caatcaaaag aaaatcttga aaaagctaaa aaagagcacg gtattaccta tggcgagtgg
     3541 gtcaatgata aggttgctta ttaccacgac tatagtaaag atggtaaaaa cgctgttgat
     3601 caagaacacg gcacacacgt gtcagggatc ttgtcaggaa atgctccatc tgaaatgaaa
     3661 gaaccttacc gcctagaagg tgcgatgcct gaggctcaat tgcttttgat gcgtgtcgaa
     3721 attgtaaatg gactagcaga ctatgctcgt aactacgctc aagctatcag agatgctgtc
     3781 aacttgggag ctaaggtgat taatatgagc tttggtaatg ctgcactagc ttacgccaac
     3841 cttccagacg aaaccaaaaa agcctttgac tatgccaaat caaaaggtgt tagcattgtg
     3901 acctcagctg gtaatgatag tagctttggg ggcaagcccc gtctacctct agcagatcat
     3961 cctgattatg gggtggttgg gacacctgca gcggcagatt caacattgac agttgcttct
     4021 tacagcccag ataaacagct cactgaaact gctacggtca aaacagacga tcatcaagat
     4081 aaagaaatgc ctgttatttc aacaaaccgt tttgagccaa acaaggctta cgactatgct
     4141 tatgctaatc gtggtacgaa agaggatgat tttaaggatg tcgaaggtaa gattgccctt
     4201 attgaacgtg gcgatattga tttcaaagat aagattgcaa acgctaaaaa agctggtgct
     4261 gtaggggtct tgatctatga caatcaagac aagggcttcc cgattgaatt gccaaatgtt
     4321 gaccagatgc ctgcggcctt tatcagtcga agagacggtc tcttattaaa agacaatccc
     4381 ccaaaaacca ttaccttcaa tgcgacacct aaggtattgc caacagcaag tggcaccaaa
     4441 ctaagccgct tctcaagctg gggtctgaca gctgacggca atattaagcc ggatattgca
     4501 gcacccggcc aagatatttt gtcatcagtg gctaacaaca agtatgccaa actttctgga
     4561 actagtatgt ctgcaccatt ggtagcgggt atcatgggac tgttgcaaaa gcaatatgag
     4621 acacagtatc ctgatatgac accatcagag cgtcttgatt tagctaagaa agtattgatg
     4681 agctcagcaa ctgccctata tgatgaagat gaaaaagctt atttttctcc tcgccaacag
     4741 ggagcaggag cagtcgatgc taaaaaagct tcagcagcaa cgatgtatgt aacagataag
     4801 gacaatacct caagcaaggt tcacctgaac aatgtttctg ataaatttga agtaacagta
     4861 acagttcaca acaaatctga taaacctcaa gagttgtatt accaagtaac tgttcaaaca
     4921 gataaagtag atggaaaaca ctttgccttg gctcctaaag cattgtatga gacatcatgg
     4981 caaaaaatca caattccagc caatagcagc aaacaagtca ccgttccaat cgatgctagt
     5041 cgatttagca aggacttgct tgcccaaatg aaaaatggct atttcttaga aggttttgtt
     5101 cgtttcaaac aagatcctac aaaagaagag cttatgagca ttccatatat tggtttccga
     5161 ggtgattttg gcaatctgtc agccttagaa aaaccaatct atgatagcaa agacggtagc
     5221 agctactatc atgaagcaaa tagtgatgcc aaagaccaat tagatggtga tggattacag
     5281 ttttacgctc tgaaaaataa ctttacagca cttaccacag agtctaaccc atggacgatt
     5341 attaaagctg tcaaagaagg ggttgaaaac atagaggata tcgaatcttc agagatcaca
     5401 gaaaccattt ttgcaggtac ttttgcaaaa caagacgatg atagccacta ctatatccac
     5461 cgtcacgcta atggcaaacc atatgctgcg atctctccaa atggggacgg taacagagat
     5521 tatgtccaat tccaaggtac tttcttgcgt aatgctaaaa accttgtggc tgaagtcttg
     5581 gacaaagaag gaaatgttgt ttggacaagt gaggtaaccg agcaagttgt taaaaactac
     5641 aacaatgact tggcaagcac acttggttca acccgttttg aaaaaacgcg ttgggacggt
     5701 aaagataaag acggcaaagt tgttgctaac ggaacctaca cctatcgtgt tcgctacacg
     5761 ccgattagct caggtgcaaa agaacaacac actgattttg atgtgattgt agacaatacg
     5821 acacctgaag tcgcaacatc ggcaacattc tcaacagaag atagtcgttt gacacttgca
     5881 tctaaaccaa aaaccagcca accggtttac cgtgagcgta ttgcttacac ttatatggat
     5941 gaggatctgc caacaacaga gtatatttct ccaaatgaag atggtacctt tactcttcct
     6001 gaagaggctg aaacaatgga aggcgctact gttccattga aaatgtcaga ctttacttat
     6061 gttgttgaag atatggctgg taacatcact tatacaccag tgactaagct attggagggc
     6121 cactctaata agccagaaca agacggttca gatcaagcac cagacaaaaa accagaagct
     6181 aaaccagaac aagacggttc aggtcaaaca ccagataaaa aaaaagaaac taaaccagaa
     6241 aaagatagtt caggtcaaac accaggtaaa actcctcaaa aaggtcaatc ttctcgtact
     6301 ctagagaaac gatcttctaa gcgtgcttta gctacaaaag catcaacaag agatcagtta
     6361 ccaacgacta atgacaagga tacaaatcgt ttacatctcc ttaagttagt tatgaccact
     6421 ttcttcttgg gattagcagc tcatatattt aaaacaaaac gccaaaaaga aactaaaaaa
     6481 tagtattact taaactgata agagcttatt taatagagag aaccaatttg gttctctcta
     6541 tttt
//
LOCUS       DATROQ010000096         6338 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.097, whole genome shotgun sequence.
ACCESSION   DATROQ010000096 DATROQ010000000
VERSION     DATROQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.097"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..830
                     /locus_tag="VBC94_001750"
     CDS             <1..830
                     /locus_tag="VBC94_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEO6980003.1"
                     /translation="NDQGIRTESFKEFAYREQLQNKNIEEVMELVDSDLNHYFERVDT
                     QVYLFNDDTLIGRYMVYLGKNYHLIEDGYNCFQAKLFLGGSVVKRVIKTYLFKKYVPY
                     GFSKYCLSIEVNSLVGLPHDIRSKKYKELPRKKLFDSLNKEQKSLIFKIFKTKPLTIT
                     PKSVLLLTQPLAQDKCYKTPTERFQSIQEQYDYFDDIVQEYRTLGYNVYLKVHPRDVV
                     DYSKLPVELLPSNVPMEIIELMSTGRFECGITHSSTALDFLTCVDKKITLVDLKDIK"
     gene            827..2227
                     /locus_tag="VBC94_001751"
     CDS             827..2227
                     /locus_tag="VBC94_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO6980004.1"
                     /translation="MNTKKLLQSGFIYTLGNLLVQGLAFITLPIYTRVISTEVYGQYS
                     LYVAWMNIIMLFIGLQTSGSLSSARVKYGEEFKSYSGSAFSVGNIWFLIILLIAFLFR
                     SFLAPLVGFSESIFLLMVCQSYASYVVTFFGQYFIQQQRSLANLILSLANAVSSVALS
                     LFLIFHWSDDFLSRVFGAFVPTIITGIVAFAYIYYHSKSFYNPKYFRFIVTVSVPLIF
                     HLLGHQLLGQLDRIMLARLYNTKEVAMYSFGYSLGMIIQIVLNSINMAWIPWFFDARK
                     EKLLQLSTYISRYLYLGVFLTLGYLTVFPELAQIMGGDKYSSSVQFISLIIVSYFLVF
                     LYTFPVNIQFFYANTTWIPIGTLLAAGVNWLLNLVLIPHYAAYGAAMATIISYLALLI
                     FHHIVSKVKYHYSDVSVRQYIILSGIVFSYAMLMNMFLDNIVIRWSLGIIILIVYSIV
                     FQKVILDLLSKKRRRR"
     gene            2227..3252
                     /gene="neuB"
                     /locus_tag="VBC94_001752"
     CDS             2227..3252
                     /gene="neuB"
                     /locus_tag="VBC94_001752"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEO6980005.1"
                     /translation="MVYIIAEIGCNHNGDINLAKKMVDVAVSCGVDAVKFQTFKAEKL
                     ISKFAPKAEYQKATTGTADSQLEMTKRLELSFEEYLEMRDYAISKGVETFSTPFDEES
                     LEFLISTDMPIYKIPSGEITNLPYLEKIGKQQKKVILSTGMAVMEEIHQAVNILRQNG
                     TTDISILHCTTEYPTPYPSLNLNVIHTLKDEFKDLTIGYSDHSIGSEVPIAAAAMGAE
                     VIEKHFTLDTNMEGPDHKASATPDILAALVKGVRIVEQALGRFEKIPDPVEEKNKIVA
                     RKSVVALKPIKKGDIYSIENITVKRPGNGISPMNWYDILGQEAQDDFEEDEVIRDSRF
                     ENQLPEL"
     gene            3329..4483
                     /gene="neuC"
                     /locus_tag="VBC94_001753"
     CDS             3329..4483
                     /gene="neuC"
                     /locus_tag="VBC94_001753"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEO6980006.1"
                     /translation="MKKICLVTGSRAEYGIMKPLIQRLSKDKEVNLQIIATAMHLEEK
                     YGYTYRQIEEDGFDIAYKVPLHLYDTDRRTVSTAMAHLQLGLTKIFDKEDYDLVIILG
                     DRYEMLPVVNVALIYNVPVCHLHGGETSLGNFDEYIRHAITKMSHLHLVSTEDFRQRV
                     IQMGEQPQFVINTGALGVENALSIPSLTKEAIEKQLGIVLEESYFVVLYHPVTFEQGK
                     SAGEQMKAVLSALSKFGVQCLFIGSNSDTGSDDIAKAINTYLINHENSYCFASLSTQL
                     YHSLIRHSLGLIGNSSSGLIEVPSLMKPTLNIGDRQKGRLHGESVVSVPVETPSVLEG
                     LSKLNEVTNFDNPYYKENASSIAYEAIKLYLKDEPSISQPFYDLKENNLK"
     gene            4480..5109
                     /locus_tag="VBC94_001754"
     CDS             4480..5109
                     /locus_tag="VBC94_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO6980007.1"
                     /translation="MKKKLAFLGAGSHADAIAPVIDPLVYDFVGFFDDKDITEHDGYP
                     VLGKLYDVLPYLEDGSIDAVFITIGDNAKRKELFEYVAKDYYDFIINIISPNALVLTP
                     DSICGRGIFIGFGAFIGSKVKLFDNNVVNTGALIEHHTVVESHCNIAPNATINGLCYI
                     REEVYVGSASVIIQTLDISSCTTIGAGAVVVKDIIEPGTYVGVPAKKIK"
     gene            5120..>6338
                     /gene="neuA"
                     /locus_tag="VBC94_001755"
     CDS             5120..>6338
                     /gene="neuA"
                     /locus_tag="VBC94_001755"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEO6980008.1"
                     /translation="MKPICIIPARSGSKGLPDKNMLFLAGKPMIFHTIDAAIESGMFD
                     KKDIFVSTDSELYREICLERGISVVMRKPELSTDQATSYDMLKDFLSDYEDNQEFVLL
                     QVTSPLRKSWHIKEAMEYYSSHDVDNVVSFSEVEKHPGLFTTLSDKGYAIDMVGADKG
                     YRRQDLQPLYYPNGAIFISNKETYLREKSFFTSRTYAYQMAKEFSLDVDTRDDFIHVI
                     GHLFFDYAIREKENKVFYKEGYSRLFNREASKIILGDSKTISISLENYHNYSQGGVTL
                     ATMLENLPNFLTANVTEAFVSIGVNDLITGHSVEEIFSNFQKLYSLLAENKIKMRLTT
                     IAYTLFRETVNNADIEKINQWLTEFCYQNQIPLLDINRFLSKDGNLNYHLTSDGLHFT
                     QEANDLLQSQYQLF"
BASE COUNT         2062 a          944 c         1186 g         2146 t
ORIGIN      
        1 taaatgatca gggaataaga actgaatcat ttaaagagtt tgcttatcgg gagcagttac
       61 aaaataaaaa tattgaagaa gtgatggagc ttgtagattc agacttaaat cactactttg
      121 agagagtaga tacacaagtc tatctattta atgatgatac tttaataggt cgctatatgg
      181 tgtatttagg taaaaattat catctaattg aagatggtta taactgtttt caagccaagt
      241 tattcttggg tggttctgtc gtaaaacgag taattaaaac ttacttattt aaaaagtatg
      301 ttccgtatgg tttttcaaaa tattgtctct caattgaggt taattcttta gtggggttac
      361 ctcatgacat aaggagtaag aaatataaag aactaccgag aaaaaaatta tttgatagtc
      421 ttaacaaaga acaaaaatca ctgattttca aaatatttaa aacaaaacca ttaactataa
      481 ctccaaagtc agtattattg ttgacacagc cacttgcaca agataaatgt tataaaacac
      541 ctacagagag gtttcaaagt attcaagagc aatacgatta ttttgacgat attgtccagg
      601 aatatagaac gttagggtac aatgtttatt taaaagttca tcctagagat gtagtagatt
      661 attccaaatt gccggtagag ctattaccat caaatgttcc tatggaaatt atagagttga
      721 tgtcaacagg tcggttcgaa tgtgggataa cacattcgtc cactgcgctg gattttttaa
      781 cttgtgttga taaaaaaata actttagtag atctgaagga tattaaatga atactaagaa
      841 attacttcaa tctggtttta tttatacact cgggaattta ttggtgcaag gattagcctt
      901 tatcacactt cccatctata ctcgggttat ctcaacagaa gtttatggcc aatatagttt
      961 atatgtcgct tggatgaata ttattatgct ttttattggc cttcagacga gcggttcttt
     1021 aagttcagcc cgggtcaaat acggagaaga atttaaaagc tattcaggga gtgccttttc
     1081 tgtaggtaat atatggtttc ttattatact tttgatagct tttctattta gaagttttct
     1141 tgcaccatta gttggttttt ctgaatctat ttttttatta atggtgtgtc aaagttacgc
     1201 tagctatgtg gtgactttct ttggtcagta ttttatacaa caacagagga gtttggctaa
     1261 tttaatatta tccttagcca atgcagtttc atctgttgca ctatctctat ttttaatttt
     1321 tcattggtcc gatgactttt tatctagggt ttttggagct tttgttccta ctataataac
     1381 tggaatagtt gcctttgctt atatttatta tcatagcaaa tctttttaca atcctaagta
     1441 ttttcggttc attgtcactg tgtctgttcc cttgattttc catttgttag gacaccagtt
     1501 gttggggcaa ttagatagaa ttatgctagc tcgtttatac aatactaaag aagttgcaat
     1561 gtatagtttc ggttattcgc ttggaatgat tattcagatt gttttgaata gtattaatat
     1621 ggcttggatt ccatggtttt ttgatgctag aaaagaaaaa ttactgcaac tttcaactta
     1681 tattagccgt tatctctact taggagtttt cttaacatta ggttatttga ctgtttttcc
     1741 tgagttagct caaataatgg gaggagacaa gtatagtagt agtgttcaat ttatttcttt
     1801 aattattgtc agctactttt tagtttttct ctatacattt cctgtaaata tccaattctt
     1861 ctatgctaat acgacatgga ttcctattgg gacattatta gctgctggtg taaattggtt
     1921 actaaacctt gtattaattc ctcattatgc agcctatggc gctgctatgg ctacaataat
     1981 atcttattta gccttgttga tttttcatca tattgtttcg aaagttaaat accattattc
     2041 agatgtctca gttaggcagt acatcatttt aagtggtata gtatttagtt atgctatgtt
     2101 aatgaacatg ttcctagata atatcgttat tagatggagt ttaggaataa ttattcttat
     2161 agtatatagc attgtttttc aaaaagtcat tttagactta ttgagtaaaa aaagaaggag
     2221 aagataatgg tttatattat tgcagagatt ggttgcaatc ataatggaga tattaatctt
     2281 gcgaaaaaaa tggtagatgt tgccgtgtct tgtggtgttg atgctgttaa atttcagact
     2341 tttaaagctg agaaacttat ttctaaattt gctcccaaag ctgaatatca aaaagcaact
     2401 acaggaacag cagacagtca acttgagatg acgaaacgtt tagagttaag ctttgaagaa
     2461 tacttagaaa tgcgtgatta tgcaatttca aaaggtgtgg agaccttttc aacacctttt
     2521 gatgaagagt cattagagtt cttaatttct acagatatgc caatttacaa aattccatca
     2581 ggagaaatca ctaatttacc ttacttagaa aagattggca agcaacaaaa gaaagttatt
     2641 ctttcgacgg gtatggcggt aatggaagag atccatcaag cggtgaatat tttacgtcag
     2701 aatggtacaa ccgacatttc tattttacat tgtacaacag agtacccaac accttacccc
     2761 tctctaaatt taaacgttat tcatactttg aaagatgaat ttaaagattt aacgataggt
     2821 tattcggatc attcaattgg atcagaagta cctatcgcag cagcagcaat gggtgcagaa
     2881 gttattgaaa aacactttac tttagatact aatatggaag gtccggatca taaagccagt
     2941 gcaacacctg atattttagc tgctttagtt aaaggggttc gcattgttga acaagcctta
     3001 ggtagatttg aaaaaatccc agatccagta gaagagaaaa ataagattgt tgctcgtaaa
     3061 tcagtcgttg ctttaaaacc aattaaaaaa ggcgatattt attcaataga aaatattacg
     3121 gtgaagcgcc caggtaatgg tatttctcct atgaactggt atgacatctt gggacaagaa
     3181 gcgcaagatg atttcgaaga ggatgaagtt attcgtgatt cacgctttga aaatcaattg
     3241 cccgagttat aataacacct aattataatc ggcaacagat gtattacacg gctaagatta
     3301 tgtaattact atatatagaa gggatacgat gaaaaaaatt tgtttagtaa ccggatcgcg
     3361 tgctgaatac ggaataatga agccattgat tcaaagatta tcaaaagata aagaagtcaa
     3421 cttacaaatt attgcaacag caatgcatct ggaagaaaag tacggctata cttatcgtca
     3481 aattgaagaa gacggttttg atattgctta taaagttccc ttacatcttt atgatactga
     3541 cagaagaact gtatctactg caatggcgca tttacaacta ggattgacca aaatttttga
     3601 caaggaagac tatgatctag tcatcatttt aggggatcgt tacgaaatgt taccagttgt
     3661 gaatgtagcg ttgatttata atgtcccagt atgccacctt catggagggg agacctcatt
     3721 aggcaatttt gatgagtata ttcgccatgc aattactaag atgagtcacc tacacttagt
     3781 ctctacagag gactttcgtc aacgtgtgat tcagatggga gaacaacctc aatttgtaat
     3841 taacacagga gctctcggag tggaaaatgc tctatcaatc ccttctctaa ccaaagaagc
     3901 aatagaaaag caattaggta ttgtactaga ggaatcctat tttgttgttc tctatcatcc
     3961 tgttactttt gaacaaggaa aaagtgctgg agaacagatg aaagcggtac tatcagccct
     4021 ttctaaattt ggtgtccagt gtctatttat aggatccaat tcagatactg gttctgatga
     4081 tattgcaaag gctatcaata cttatttgat aaatcatgaa aatagttatt gctttgcttc
     4141 tttatcaacc caactttatc attccttaat tcgtcattca ctaggactga ttggtaattc
     4201 gtcttctggt ttaatagaag ttccttcttt gatgaaacct actcttaata ttggtgatcg
     4261 ccaaaaaggt aggctacatg gagaaagtgt agtaagtgta ccagtcgaaa ccccctctgt
     4321 attggaggga ttatcaaaat tgaatgaagt gaccaacttt gacaatccct actacaaaga
     4381 gaatgcttca tctattgcat atgaagcaat aaagctttat ttgaaagatg aaccttcaat
     4441 ttcccaacca ttttatgatt taaaggagaa taatctaaaa tgaaaaaaaa gttggcattt
     4501 ttaggcgcag gaagtcatgc tgatgcgatt gctccggtta ttgatcctct tgtgtatgat
     4561 ttcgtaggtt tttttgatga taaagatatt acggagcatg atggttatcc tgttcttgga
     4621 aaactttatg atgtgctacc ttaccttgaa gatggctcaa tagatgcagt atttattaca
     4681 ataggtgata atgctaaaag gaaagaacta tttgaatatg tagcaaagga ttattatgac
     4741 tttattatta acatcattag tcccaatgct ttagtattga caccagatag tatttgtggg
     4801 cgtggtatct ttattggttt tggggctttt ataggttcta aagtgaagct gtttgataac
     4861 aacgttgtta atacaggagc gctcattgaa catcatactg ttgtagaatc acactgtaat
     4921 atagcaccta atgctaccat aaatggtctt tgttatatta gagaagaagt ttatgtaggt
     4981 agtgccagtg ttattattca aaccttggat atttcatcgt gtacgacaat tggcgcagga
     5041 gctgttgttg taaaagatat tatagaacct ggaacctatg taggggttcc agccaaaaaa
     5101 ataaaataaa ggatatgtta tgaagccaat ttgtattatt cctgcgcgat cagggtcaaa
     5161 aggattacca gataagaata tgttattcct agcaggtaaa cccatgattt tccatactat
     5221 agatgctgca attgaatctg gaatgtttga taagaaagat atttttgtta gtacagattc
     5281 agaattgtac agagagattt gtttagaacg cggtatttca gtggtgatga gaaaaccgga
     5341 actttcaact gatcaggcaa cttcgtatga tatgttaaaa gattttttat ctgactatga
     5401 agataatcag gagtttgtgt tacttcaagt aacctctcca ctaagaaaat catggcatat
     5461 aaaggaagca atggagtatt attcttcaca tgatgttgac aatgttgtaa gtttttctga
     5521 agttgagaaa caccctggtc tgtttacgac attgtctgat aaaggctatg ctatagatat
     5581 ggtgggagca gataaaggtt atcgtcgcca agatttacaa cctttatact atccgaacgg
     5641 cgctattttt atttctaata aagaaactta cttaagggaa aaaagctttt tcacctctag
     5701 gacatatgct tatcaaatgg caaaggaatt ttcattagat gttgatacga gagatgattt
     5761 tatccacgtc atcggtcacc tctttttcga ttatgccatt agggaaaaag agaataaagt
     5821 tttttataaa gaaggctata gtcgtttgtt caatagagaa gcttcaaaga taattttagg
     5881 tgattcaaaa acgatatcta tctcactaga aaattaccat aattattccc aaggtggtgt
     5941 aacattagca acgatgttgg aaaacttacc taactttttg acagctaatg taacagaggc
     6001 ttttgtttct ataggggtaa atgatctcat tacaggtcat agtgtggaag aaatattcag
     6061 caatttccaa aaactctact cattattagc tgagaataaa ataaagatga gattgacaac
     6121 tattgcttac acccttttta gagaaactgt caataatgca gatattgaga aaatcaatca
     6181 atggctaaca gaattttgtt atcaaaatca gattccactg ttagatatta atagattttt
     6241 atcaaaggat ggtaatctaa attatcattt aactagtgat ggattacatt ttactcaaga
     6301 ggctaatgat ttgttacaaa gtcaatatca attatttg
//
LOCUS       DATROQ010000097         6091 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.098, whole genome shotgun sequence.
ACCESSION   DATROQ010000097 DATROQ010000000
VERSION     DATROQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.098"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            115..1260
                     /locus_tag="VBC94_001756"
     CDS             115..1260
                     /locus_tag="VBC94_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6980009.1"
                     /translation="MIYFDNSATTIPYPEVLRTYQEVASKIYGNPSSLHELGTTSSRI
                     LEASRKQIASLLELKANEIFFTSGGTEADNWVIKGLAFEKQHFGNHIIVSDIEHPAVK
                     ESAKWLGEYGFEIDYAPVDDKGFVDVEALVKLIKPETILISIMAINNEIGSIQPIKAI
                     SDLLSDKPTISFHVDAVQAIGKIPTKDYLTERVDFASFSSHKFHGVRGVGFLYIKEGK
                     RISPLLTGGGQESDLRSTTENVAGIAATAKALRMVMDKEVVAIPKISKMKTIIHDELA
                     KYEDITLFSGKEGFSPNIITFGIKGVRGEVLVHAFEGHDIFISTTSACSSKAGKPAGT
                     LIAMGISTKLAQTAVRISLDDDNDMGQVEQFLTIFKQVYEKTKKVRG"
     gene            1262..2476
                     /gene="thiI"
                     /locus_tag="VBC94_001757"
     CDS             1262..2476
                     /gene="thiI"
                     /locus_tag="VBC94_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEO6980010.1"
                     /translation="MQYSEIMIRYGELSTKKKNRMRFINKLKNNMEHVLSIYPDVSVK
                     TDRDRGHVYLNGTDYHEVAESLKEIFGIQAFSPSFKVEKNVDTLVKAVQEIMTSVYKD
                     GMTFKITAKRSDHSFELDSRALNHTLGDAVFSVLPNIKAQMKQPDINLKVEIRDEAAY
                     ISYEDIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAHDLTRKLTKFGGNIQFIEVPFTEIQEEIKEKAPEAYLMTLTRRFMMRITDRIR
                     EDRNGLVIINGESLGQVASQTLESMQAINAVTATPIIRPVVTMDKLEIIDIAQKIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNTEQYEKRMDVEGLVERAVAGIMVTTIQPQAD
                     SDDVDDLIDDLL"
     gene            2578..3756
                     /locus_tag="VBC94_001758"
     CDS             2578..3756
                     /locus_tag="VBC94_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEO6980011.1"
                     /translation="MARIPYQKTLIFCVAVIIAIFILGLSKDLAQSKGQKVANNNTVK
                     TARVVANGDILLHDVLYASARQPDGTYNFTPYFKEVKSWIESADLAIGDYEGTISSEY
                     PLAGYPLFNAPNEIATTMKETGYDVVDLAHNHILDSQLAGAINTVKTFNRLGLDTIGV
                     YLKDRNKEDILIKRVNGIKIAILGYSYGYNGMEANVSKSDYEKHMSDLDTKKIKQDIK
                     KAEKEADITIVMPQMGIEYQKKPTTEQVILYHSMIKWGADIIFGGHPHVVEPSEVIKK
                     DGQKKFIIYSMGNFISNQRLETVDDIWTERGLLMDVTIEKKGQKTVIKKVKAHPTLVE
                     AKANGRYVEEGFPAYDYRTLVLEDYIEGGKKRSTIDQTMKDKVDKAYKEMNELVNLKW
                     "
     misc_feature    3832..3911
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            3946..4260
                     /gene="rplU"
                     /locus_tag="VBC94_001759"
     CDS             3946..4260
                     /gene="rplU"
                     /locus_tag="VBC94_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEO6980012.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKLDVEAGAEVTFNEVVLVG
                     GETTKVGTPVVEGATVVGTVEKQGKQKKVVSYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            4267..4605
                     /locus_tag="VBC94_001760"
     CDS             4267..4605
                     /locus_tag="VBC94_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEO6980013.1"
                     /translation="MIKATFTRNQSGYLYSAEISGHAGSGEYGFDVICAAVSTLSINF
                     INSLEALTTCQAQLIINDVEGGYMKIDLSSIPQHKEDKVQLLFESYLLGMTNLSKDSS
                     EFVSTVVMTH"
     gene            4627..4920
                     /gene="rpmA"
                     /locus_tag="VBC94_001761"
     CDS             4627..4920
                     /gene="rpmA"
                     /locus_tag="VBC94_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEO6980014.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGANVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            5136..6041
                     /locus_tag="VBC94_001762"
     CDS             5136..6041
                     /locus_tag="VBC94_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6980015.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAAKLFVSQPSLSVAVRDLETELG
                     FQIFTRTTTGAVLTNQGMTFYENALEVVKSFDSFEKQFSQSEATEQEFSIASQHYDFL
                     PPLITAFSKCNDNFSYFRIFESTTIRILDEVAQGNSEIGIIYINSQNKKGLLQRLDKL
                     GLEFVELIPFKTHIYLGKDHPLASKTSLIMTDLEGLPTVRFTQDRDDYRYYSENFVEV
                     LDSSVTYNVTDRATLNGILERTQAYATGSGFLDSRSVNGITVIPLEDHLDNQMIYIKR
                     KDRNLSQMALKFVAVMEEYFKGFNS"
BASE COUNT         2057 a          901 c         1237 g         1896 t
ORIGIN      
        1 tgtgataaat tatgagaatt atcataaata ttgttttggg atgttcacaa actaatataa
       61 atagtaggaa aattatgcta taataacact gattgacttt agaaggacag atttatgatt
      121 tattttgata attcggcaac tactattccc tatccagaag ttttaaggac ttatcaagaa
      181 gtagctagta aaatctatgg aaacccctca agtttacatg agctaggaac aacatcttct
      241 cggattttag aggctagccg taaacaaatt gctagtttac ttgagcttaa agcaaatgaa
      301 attttcttta cctctggtgg tacagaggcc gataattggg ttattaaggg tcttgcgttt
      361 gagaaacaac attttggaaa tcatattatt gtttctgata tcgaacaccc agctgttaaa
      421 gagtctgcta aatggcttgg agagtatggg tttgagattg attatgcgcc agtagatgat
      481 aaaggatttg tagacgtaga agcactggtc aaattaatta aaccagaaac gattcttatt
      541 tcaattatgg caataaacaa tgaaattggt tctattcagc caataaaagc tatttcagac
      601 ttgttgtcag ataaacctac tatatcgttt catgttgatg ctgttcaagc aataggtaag
      661 ataccgacta aagattattt gactgagcga gttgattttg catctttttc tagtcataaa
      721 tttcacggcg ttcgtggggt tggctttcta tatattaaag aaggaaaacg tatttcacca
      781 ttactaacag gtggtggaca agaatctgat ttacgttcaa ctactgagaa tgtcgcaggg
      841 attgcagcta ctgctaaagc tttacgaatg gttatggaca aggaagtagt agctattcct
      901 aaaataagta aaatgaaaac cattattcat gatgaattag ccaaatatga ggatattacg
      961 ttattttcgg gtaaggaagg tttttcacct aacattatca cgtttggtat caagggtgtc
     1021 cgaggagaag ttcttgtcca tgcctttgag ggacatgata tttttatctc gacaactagc
     1081 gcttgctctt ctaaagcagg taaaccagca ggaactctta tagcaatggg aatttcaact
     1141 aagttagccc aaactgctgt acgtatcagc ttagatgatg ataatgatat gggacaagta
     1201 gagcaatttt taactatttt taaacaagtt tatgaaaaaa ctaagaaagt tagaggataa
     1261 gatgcagtat tcagaaatta tgattcgtta tggagaactc tctactaaga agaaaaaccg
     1321 tatgcgcttc atcaataagt taaaaaataa tatggagcat gtactctcca tttatccaga
     1381 tgtttcagta aaaacagatc gtgatagagg acatgtatat ctcaatggta cagattatca
     1441 tgaagttgca gagtccttaa aagagatttt tggtatccaa gctttttctc catcttttaa
     1501 agtagaaaaa aatgttgata cattggtaaa agctgtccag gaaattatga cttccgttta
     1561 taaagatggg atgactttta aaattaccgc aaaacgtagt gaccactcat ttgaattgga
     1621 tagccgtgct ctaaatcata ctttaggaga tgccgttttt tcagtcttgc caaatattaa
     1681 ggctcagatg aagcaaccag atatcaatct taaagtcgag atacgagatg aggctgctta
     1741 tatttcatat gaggatatta ggggtgcagg aggattaccg gtaggaacat ctgggaaagg
     1801 gatgctgatg ttgtctggtg ggattgattc tccggtagca ggttacctag cgttaaaacg
     1861 tggtgtagat atagaagcag tccattttgc aagtcctcct tatactagcc caggtgcatt
     1921 gaaaaaagca catgatttaa cacgtaaatt gacaaaattt ggtggtaata ttcaatttat
     1981 tgaagttcca ttcacagaaa ttcaagagga aattaaggaa aaagctcctg aagcctactt
     2041 gatgacgtta acacgtaggt ttatgatgcg tattacagat cgtattcgtg aggaccgaaa
     2101 tggtcttgtt attattaacg gtgaaagttt agggcaggtg gcaagccaaa cgctagaaag
     2161 tatgcaagct attaatgctg tcactgcaac accgattatt cgtcctgtgg tcacgatgga
     2221 taagctagaa attattgata ttgctcaaaa aatagatact tttgatattt caattcaacc
     2281 atttgaggat tgctgtacaa tttttgcacc agatcgccca aaaactaacc ctaaaattaa
     2341 gaatacagaa cagtatgaga aacgtatgga tgtagaaggt ctggtagaga gggcagttgc
     2401 agggattatg gtaactacta ttcaacctca agcagatagt gatgatgttg atgacttgat
     2461 tgacgattta ttataatgaa tttttaaact acagatttac tctgtagttt tttattaaaa
     2521 tgattgtgct gtcataaggt tataaaaaat gctataatac gaataaagga ggcaagtatg
     2581 gcaagaatcc cttatcaaaa aacacttatt ttttgtgtag ccgttattat tgctattttt
     2641 attttggggt tatctaagga tttagcacaa tctaaagggc aaaaggttgc aaataataat
     2701 actgtaaaaa cagcacgtgt ggttgcaaac ggagatattt tgctgcacga tgtactatat
     2761 gccagtgcgc gccaacccga cggcacttat aattttactc cttattttaa agaggtaaaa
     2821 tcttggattg agagcgctga tttagcgatt ggtgattatg aagggacgat tagttcagaa
     2881 tatcctttag cgggctatcc tttatttaat gctcctaatg agattgcaac tactatgaaa
     2941 gaaactggat atgatgtcgt tgacttagca cataatcata ttctggattc acaactggca
     3001 ggtgctatca atactgtcaa gacttttaat cgtctgggat tggatactat tggggtttat
     3061 ttaaaggata gaaataaaga agatatctta attaagcgtg taaatggtat taaaattgct
     3121 attttaggtt attcgtatgg ctacaatggt atggaagcta atgtttcaaa gagtgactat
     3181 gaaaaacata tgtctgattt agatactaaa aagattaagc aagatattaa aaaggctgaa
     3241 aaagaagcag atatcaccat tgtcatgcca cagatgggga tagaatatca aaaaaaacct
     3301 actacagaac aagtaatact ttatcatagt atgattaaat ggggggcaga tattatattc
     3361 ggaggacacc ctcacgttgt tgaaccgtct gaagtgataa aaaaagatgg ccagaaaaaa
     3421 tttattattt actctatggg aaattttatt tcaaatcagc gtttagaaac ggttgatgat
     3481 atctggacag aaagaggatt attaatggat gtaaccatag agaaaaaagg tcaaaaaaca
     3541 gtaattaaaa aagttaaggc gcatccaaca ctagtagaag caaaagcaaa tggccgttat
     3601 gttgaagaag gctttccagc ttatgattat aggacgctag ttttagagga ttatattgaa
     3661 ggtggtaaga aacgttctac aattgatcag actatgaaag ataaagttga taaagcatat
     3721 aaggaaatga atgagttagt taatttgaag tggtaaagtt gataatttta ttttgtttta
     3781 tcttgcataa aaaaagagtt tatgataaaa tttgaatgtt gactaaatgc acatcctgtg
     3841 caaccgcgcg aaaatcgtta gattaagtag ggcaaggctc tcacgattta taggcgagtc
     3901 ttcacagagg gaaacctcac aagaaatttt tataggaggt gcatcatgag cacatacgca
     3961 atcatcaaaa ctggtggtaa acaagttaaa gttgaagtag gacaagctat ctacgttgaa
     4021 aaacttgacg ttgaagcagg agcagaagtt acatttaacg aagttgttct tgttggtggt
     4081 gaaacaacta aagttggtac tccagttgtt gaaggagcta ctgttgttgg tactgttgaa
     4141 aaacaaggca aacaaaagaa agttgtttct tacaaataca aacctaaaaa aggtagccac
     4201 cgtaaacaag gtcaccgtca accttataca aaagttgtta tcaacgcaat caacgcttaa
     4261 tttttcatga ttaaagcaac ttttactcgt aatcaatcag gctatcttta tagtgctgaa
     4321 atcagcggtc atgctggtag tggcgagtac ggctttgatg tgatttgtgc agctgttagt
     4381 actttatcaa ttaattttat taattcatta gaggctttaa caacctgcca agcgcaattg
     4441 attatcaatg acgttgaagg tggttatatg aagatagatt tatcttctat accacaacat
     4501 aaagaggata aggttcaatt attatttgag tcttaccttc ttggaatgac taatctgtct
     4561 aaagattctt ctgaatttgt tagtacagta gtcatgacac actaatttaa gagaggacac
     4621 aacattatgt taaaaatgaa tcttgctaac ttgcaacttt tcgcccacaa aaaaggtgga
     4681 ggttctacat caaacggacg tgattcacaa gcaaaacgtc ttggagctaa agcagctgac
     4741 ggacaaacag tatcaggtgg atcaattctt taccgtcaac gcggtacaca catctatcca
     4801 ggtgctaacg ttggacgtgg tggggacgat acactttttg ctaaagttga aggtgttgtt
     4861 cgtttcgaac gtaaaggacg cgataaaaaa caagtgtctg tttacccaat cgcaaaataa
     4921 tgcctagtta agactttatg actattcata aggtctttta ttattgtcag agctttcatt
     4981 ttgtaacaaa agacaatatg ttaagagatt tatcattaat actcataaac tttgttgttc
     5041 ttttatagtg aaatagtcct aattcatgaa gaattttccg caaggatagt tattttttgg
     5101 tataatgaag ggtactaaaa tgaggaagtg ataaaatgaa tattcaacaa ctgcgttatg
     5161 ttgttgctat cgcaaatagt ggtacttttc gtgaagcagc tgcaaaacta tttgttagtc
     5221 agccaagttt atctgttgcc gttcgtgatt tggaaacaga attagggttt cagattttta
     5281 cgcgtacaac tactggtgca gtgttgacca atcaaggcat gactttttat gagaatgctt
     5341 tagaagttgt gaaaagcttt gattcattcg aaaaacagtt ttcgcaatct gaagctacag
     5401 agcaagaatt ctcaatagca agtcaacatt atgattttct acctccttta atcacagcgt
     5461 tttcaaaatg taatgataat tttagctatt tccgtatttt cgaatcaaca acaatacgta
     5521 tcttagatga agttgctcaa gggaatagcg aaataggtat catttatatc aatagtcaaa
     5581 ataaaaaagg tctacttcag cggttggaca aattggggtt agagtttgta gagcttattc
     5641 cttttaaaac tcatatttat cttgggaaag accatccctt ggctagtaaa acatctctga
     5701 taatgacaga tttagaagga ttaccaaccg tacgttttac acaggataga gatgattatc
     5761 ggtattactc ggagaatttt gtggaagtac ttgatagttc tgttacctat aatgttactg
     5821 atagagcaac attaaatggt attttagaaa gaacccaagc ttatgcgacg ggctcaggat
     5881 tcttggatag ccgcagtgtg aatggtatca ctgttattcc attagaagat catctagata
     5941 accaaatgat ttatatcaaa cgaaaagatc gtaatttatc tcagatggct ttaaaatttg
     6001 ttgctgttat ggaagagtat tttaagggat ttaattcata ggagaagtaa tgagaaagat
     6061 tattatacca ataataacta ttttgttgat t
//
LOCUS       DATROQ010000098         6087 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.099, whole genome shotgun sequence.
ACCESSION   DATROQ010000098 DATROQ010000000
VERSION     DATROQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.099"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            79..366
                     /locus_tag="VBC94_001763"
     CDS             79..366
                     /locus_tag="VBC94_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEO6980016.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILSDNGATVVESKDWE
                     KRRLAYEIQDFTEGLYHIVNVEAEDAVALNEFDRLSKINGDILRHMIVKVD"
     gene            378..869
                     /locus_tag="VBC94_001764"
     CDS             378..869
                     /locus_tag="VBC94_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6980017.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFSLAVNRNFKNQSGER
                     EADFINCVIWRQQAENLANWAKKGALVGITGRIQTRNYENQQGQRVYVTEVVAESFQL
                     LESRATREGGSPNSYNNGGYNNAPSNNSYSASSQQTPNFSRDESPFGNSNPMDISDDD
                     LPF"
     gene            914..1153
                     /locus_tag="VBC94_001765"
     CDS             914..1153
                     /locus_tag="VBC94_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEO6980018.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            1323..2267
                     /locus_tag="VBC94_001766"
     CDS             1323..2267
                     /locus_tag="VBC94_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6980019.1"
                     /translation="MKQMFLSTAIEFKEIETFEPGAWINLVNPSQEESEQVADQFGID
                     IDDLRAPLDVEETSRISVEDDYTLVIVDVPTYEERNNKSYYMTIPMGIIVTDNAVITT
                     CLEHLTLFDHFYRRRVKNFYTFMKTRFVFQLLYRNAELYLQALRTIDRQSDKIEAQLE
                     SATRNEQLIDMMELEKSIVYLKASLKFNERIVKKLTSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMANIYENVLNAMTETTASIIGNNQNTIMKTLALVTMTLDIPTVIFSAYGMNF
                     KNNWMPLNGLAHGFIYVVLLAFLMSSFVVFYFIRKKWF"
     gene            2292..2963
                     /locus_tag="VBC94_001767"
     CDS             2292..2963
                     /locus_tag="VBC94_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HEO6980020.1"
                     /translation="MTLQDLTKKNQEFVHIATNQLLADGKSDAEIKAILEEHLPEIID
                     NQKKGITARSLLGAPTTWAASFTERPEDKARVSVQKNTNPWLMWLDTSLLFLGLVTAL
                     NGLMLLFGQSNVNTGLISILTLGFGGGAAMYVTYYYIYRHMGKPKSERPGWLKSFAVL
                     ALVMLVWFALFAVVPLLPATINPKLPEVVLFIIALASFGLRFYLQRKYNIQSSMAPVA
                     PQQRR"
     gene            3095..5923
                     /gene="uvrA"
                     /locus_tag="VBC94_001768"
     CDS             3095..5923
                     /gene="uvrA"
                     /locus_tag="VBC94_001768"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEO6980021.1"
                     /translation="MQDKLMIRGARAHNLKNISVDIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSVEQIVDKVLALPERTKMQILAPIIRRKK
                     GQHKSTFEKIQKDGYVRVRIDGDIHDVTEVPELSKSKMHNIDIVVDRLINKEGIRSRL
                     FDSVEAALRLSDGYVVIDTMDGNELLFSEHYSCPECGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDIDLVIPDRSKTLREGALVPWNPISSNYYPTMLEQAMTQFGVDVDTP
                     FEKLSKAEQDLALYGSGEREFHFHYINDFGGERNIDLPFEGVVNNINRRYHETNSDYT
                     RNVMREYMNELKCNTCHGYRLNDQALCVRVGGEEGLNIGQVSDLSIADHLELLETLRL
                     SSNEQLIARPIIKEIHDRLSFLNNVGLNYLNLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYVLDEPSIGLHQRDNDRLIDSLKKMRDLGNTLIVVEHDEDTMMAADWLIDVGPGA
                     GAFGGEIVASGTPKQVAKNTKSITGQYLSGKKVIPVPSERRVGNGRFLEIKGAAENNL
                     QNLDVKFPLGKFIAVTGVSGSGKSTLINSILKKAVAQKLNRNSDKPGKYVSLEGIEYV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     ESCSGDGIIKIEMHFLPDVYVPCEVCHGTRYNSETLEVHYKEKNIAQILDMTVNDAVT
                     FFAAIPKIARKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHADDIARLLKVLDRFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGIGGGQ
                     IVAIGTPEEVAENPKSYTGYYLKEKLAR"
BASE COUNT         1942 a         1011 c         1223 g         1911 t
ORIGIN      
        1 taataatatc ttgtgagcat aacctcactt actcatgaaa aattcatgag tcattagacc
       61 aaaaggagga acatatcaat ggctaaatac gaaattcttt atattattcg tccaaacatt
      121 gaagaagaag ctaaaaacgc tttggtagca cgttttgatt ctatcttatc tgacaacggt
      181 gcaactgttg ttgaatcaaa agattgggaa aaacgtcgtc ttgcatacga aatccaagat
      241 ttcactgaag gactttacca tatcgttaac gttgaagctg aagatgcagt agctcttaat
      301 gagtttgatc gtctttcaaa aatcaacggt gacattcttc gtcacatgat cgttaaagta
      361 gactaagaag gtagtttatg attaataatg tagtacttgt aggtcgcatg acccgtgatg
      421 cagaacttcg ttatacacca agtaatcaag cggtagccac tttttcactt gcagttaatc
      481 gtaattttaa aaatcaatct ggcgaacgtg aggctgattt tattaactgt gttatttggc
      541 gccaacaagc tgaaaacttg gctaactggg caaaaaaagg tgctttggtt ggaattacag
      601 gtcgtatcca aacgcgtaat tatgaaaacc aacaaggtca acgtgtctat gtaacagaag
      661 ttgttgcgga aagtttccaa ttattggaaa gtcgtgctac acgagaaggt ggttcaccta
      721 actcttataa taacggtggc tataacaatg ctccgtcaaa taatagttat tcagcttctt
      781 ctcaacaaac acctaatttt agtcgtgatg agagtccgtt tggtaactca aatccaatgg
      841 atatttcaga tgatgatcta ccattctaat ataatagatg gacagaattt aaaactataa
      901 aggagaaata aacatggctc aacaacgtcg tggcggattc aaacgccgta aaaaagttga
      961 ttatatcgca gctaacaaga ttgaatatgt tgattacaaa gatacagagc ttcttagccg
     1021 tttcgtttca gaacgtggaa aaattttacc acgtcgtgta acaggaactt cagctaaaaa
     1081 ccaacgtaaa gtaacaacag caatcaaacg tgctcgtgtt atggctctta tgccttacgt
     1141 aaatgaagat taatataaaa tagccctagc ggctattttt ttattctttt taccattatt
     1201 attggtattt acgagtcaaa taatagaaaa atttaggata ccaccgtatt aaccgtgcaa
     1261 aacaaatggt tttcctgtat aatactaagg tatggatttt ttataacaaa ggaggaatcg
     1321 ccatgaagca aatgtttctt tcaacggcaa ttgaatttaa agaaattgaa acattcgagc
     1381 caggtgcttg gataaatctg gttaatccct cccaggaaga atccgaacaa gtagctgatc
     1441 aatttggtat tgatattgat gatttacgtg ctcccctcga tgtggaagaa acttcccgta
     1501 tctcggttga agatgattat actttagtta ttgttgacgt tcctacctat gaagaacgca
     1561 ataacaaaag ttattatatg actattccta tgggcattat cgttactgac aacgctgtta
     1621 ttactacttg cctagaacat ttgacgctgt ttgatcactt ttatagacga cgtgtcaaaa
     1681 atttttatac ctttatgaag acacgttttg ttttccaatt gctttatcgc aatgccgaat
     1741 tatacttaca ggctttgcgt actatcgata ggcagagcga taaaattgaa gcacaactag
     1801 agagtgcaac tcgaaatgag caattgatcg atatgatgga attggaaaaa tctattgtct
     1861 atctaaaggc ctctttaaaa tttaatgagc gaatcgtcaa aaaattgacc agtagtacca
     1921 gtagtttaaa aaaatacatt gaagatgaag atctgcttga agatacattg atcgaaactc
     1981 agcaggctat tgaaatggca aatatctatg aaaatgtctt aaatgccatg acagaaacga
     2041 ctgcttcaat catcggtaat aatcagaata ccattatgaa aaccttagcc ctagtaacta
     2101 tgacattgga tattccaacg gttatttttt cggcgtatgg gatgaacttt aaaaataatt
     2161 ggatgccttt gaatggtctc gcacatggct ttatctatgt tgttcttttg gcctttttga
     2221 tgagttcatt tgttgtattt tattttattc gtaaaaaatg gttttaatag tatattaatc
     2281 aaggagttca catgacttta caagacttaa ctaaaaagaa tcaagagttt gttcatattg
     2341 caactaatca acttttagct gatggaaaat cagatgcaga aatcaaagct attttagaag
     2401 aacatctccc agagattata gataatcaga aaaaaggaat taccgcacgc tcactcttag
     2461 gagcaccaac tacttgggca gcctcattca ctgagcgacc tgaggataaa gctagagtat
     2521 ctgtacaaaa aaataccaat ccttggttga tgtggttaga tacttcctta ctatttcttg
     2581 gcttagtaac tgctttgaat ggtttaatgt tgctatttgg tcaatctaat gtcaatacag
     2641 ggcttatttc aattttaact cttggttttg gtggtggtgc ggccatgtac gttacttatt
     2701 actatattta tcgccatatg ggtaaaccaa aatctgaaag accaggctgg cttaaatctt
     2761 tcgcagtact ggcactagtt atgttagttt ggtttgcctt atttgcagta gtacctcttc
     2821 tacctgcaac tattaatcct aaacttcctg aggttgtttt gtttattatt gctttagcat
     2881 cgtttggttt gcgcttttat ttacaacgta aatataatat tcaaagctca atggcaccag
     2941 tggcaccaca acaaagaaga taataacaaa gagtatgaac gtattagttc atctcttttt
     3001 tctatgaaat ttaaaactaa aaaaatactg actttattaa aatttgatat aataagtcaa
     3061 cactattcaa aaaggagtgc tgaggcacaa cattatgcaa gataaattaa tgataagagg
     3121 agcaagagct cacaatttaa aaaatataag tgtcgacatt ccacgagaca agttagttgt
     3181 cgttacggga ctatcaggat caggtaagtc tagtttggcc tttgacacta tctatgcaga
     3241 aggacaacgt cgttacgttg aaagtctttc tgcttacgcg cgccaatttt tgggaaatat
     3301 ggaaaagcct gatgtagatt ctattgatgg ccttagtcca gctatttcta ttgatcagaa
     3361 aacaacaagt aagaatcctc gctctactgt tgggactgta acagaaatta acgattattt
     3421 acgtttgctt tatgctcgtg ttggaacccc atattgtatc aatggtcacg gagccatcac
     3481 agcatcttct gtagagcaaa ttgtggataa ggtactagcg cttccagagc gaacaaagat
     3541 gcaaatttta gccccaatta tacgtcgaaa aaaaggacaa cacaaatcaa catttgagaa
     3601 aatacaaaaa gatggttacg ttcgtgttcg tattgatggt gatattcacg atgttactga
     3661 ggtgcctgaa ttatcaaaaa gtaagatgca caatattgat attgtagtag atcgattaat
     3721 taataaagag ggcattcgtt cccgcttatt tgattccgtt gaagcggctc tacgtttgtc
     3781 ggatggttat gtcgttattg atacaatgga tggcaatgag ttattgtttt cagagcatta
     3841 ttcttgtcct gaatgtggtt ttactgttcc tgaattagag ccacgccttt tttcttttaa
     3901 tgctcccttt ggttcttgtc cgacctgtga tggcttagga attaagttag aagtagatat
     3961 agatttggtt attccagata gaagtaagac ccttagagaa ggggccttag ttccttggaa
     4021 tccaatttca tcaaattatt atccaacgat gctagagcaa gctatgactc aatttggtgt
     4081 tgatgtggat acgccatttg aaaaattaag caaggcggag caggacctag ccctatatgg
     4141 ttcaggcgaa cgagagttcc atttccatta tattaacgat tttggaggcg agagaaatat
     4201 tgatttgcct tttgaagggg ttgtcaataa tattaatcgt cgttaccatg aaactaatag
     4261 cgattacaca cgcaatgtta tgcgagaata tatgaacgag ctaaaatgta acacttgtca
     4321 tggctatcgt ctcaatgatc aagcactttg cgtaagagtt gggggagaag aaggtcttaa
     4381 tattgggcaa gtctcagatt tatcgatagc agaccattta gaacttttag aaacattgcg
     4441 tttatcttca aatgaacaat taattgctcg accaattatc aaagaaatcc atgaccgtct
     4501 aagtttctta aataatgttg gacttaatta tttaaatctg tcacgttctg caggaacact
     4561 ttccgggggc gagagtcaac gtattcgttt ggctactcaa attgggtcta atttatcagg
     4621 tgtcctttat gtacttgatg agccgtccat tggacttcat cagcgagaca atgaccgttt
     4681 gattgatagt cttaagaaaa tgcgtgattt gggtaatacg cttattgttg ttgaacatga
     4741 tgaagataca atgatggcag ctgattggtt aatcgatgtg ggacctggcg ctggtgcgtt
     4801 tggtggtgag attgtggcct ctggtacacc gaagcaagta gctaaaaata ctaaatctat
     4861 tacaggacaa tatttgtcag gtaagaaagt gattcctgtg ccatctgaac gaagagtagg
     4921 taatggtcgc tttttagaaa tcaaaggtgc ggcagaaaac aacctacaaa atttagatgt
     4981 caagttccct ctcggtaaat ttattgccgt aactggtgtc tcaggttctg gaaaatcaac
     5041 cttaattaat agtattttga aaaaagctgt tgcgcagaag cttaaccgta actcagataa
     5101 accaggtaaa tatgtttctt tagaaggcat tgaatatgtt gatcgattga ttgatattga
     5161 tcaaagtcca attggtcgga ctccacgttc taatcccgca acctatacag gtgtttttga
     5221 tgatattcgt gatctttttg ctcaaactaa tgaggcaaag attcgaggtt ataaaaaagg
     5281 acgcttctca tttaacgtca aaggtggacg ttgtgagtct tgttctggcg atggtatcat
     5341 caaaattgag atgcattttt taccagatgt ctacgttcct tgtgaagtat gccacggcac
     5401 gcgctataat agtgagactc tggaagtaca ttacaaagaa aaaaatattg cacaaattct
     5461 tgatatgaca gtgaatgatg cagttacatt ttttgcagct attcctaaaa ttgctcgtaa
     5521 gttacagact attaaggatg ttggtttggg atatgtcact ttggggcaac cagctacaac
     5581 cttatcagga ggagaagctc agcgaatgaa gctggcaagt gaattacata agcgttcgac
     5641 aggtaagagt ctttacatat tagacgaacc aacaacgggg cttcatgcag atgatattgc
     5701 tcgtttactt aaggttttgg atcgttttgt agatgatggt aatacagtgc ttgttattga
     5761 gcataacctt gatgttatta aaactgctga ccacattatt gatcttggcc ctgaaggagg
     5821 tattggtggc ggacaaattg tagctatcgg aacaccagaa gaagttgctg aaaatcctaa
     5881 atcctataca ggatattatt taaaagagaa gttagcaaga tagtttattg ggagctgagt
     5941 aatattactc agttcctttt tgtaagctaa cattgcgtag aagtcattaa atggagaacg
     6001 ctttattagg aaaaatagtg gggagatatt ttaaaaaaag gaaataatga tgttttgaaa
     6061 aaaatagttt ctatgagaat ctttttc
//
LOCUS       DATROQ010000099         6080 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.100, whole genome shotgun sequence.
ACCESSION   DATROQ010000099 DATROQ010000000
VERSION     DATROQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.100"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            103..1248
                     /locus_tag="VBC94_001769"
     CDS             103..1248
                     /locus_tag="VBC94_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6980022.1"
                     /translation="MGQEPIIEYQNINKVYGENVAVEDINLKIYPGDFVCFIGTSGSG
                     KTTLMRMVNHMLKPTNGTLLFKGKDISTINPIELRRRIGYVIQNIGLMPHMTIYENIV
                     LVPKLLKWSEEAKRAKARELIKLVELPEEYLDRYPSELSGGQQQRIGVIRALAADQDI
                     ILMDEPFGALDPITREGIQDLVKSLQEEMGKTIILVTHDMDEALKLATKIIVMDNGKM
                     VQEGTPNDLLHHPATSFVEQMIGEERLLHAQADITPVKQIMLNNPVSITTEKTLTEAI
                     TLMRQKRVDSLLVTDNGKLIGFIDLESLSSKYKKDRLVSDILKHTDFYVMEDDLLRNT
                     AERILKRGLKYAPVVDHENNLKGIVTRASLVDMLYDIIWGDTETEDQ"
     gene            1248..1883
                     /locus_tag="VBC94_001770"
     CDS             1248..1883
                     /locus_tag="VBC94_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6980023.1"
                     /translation="MVNFLSQYGMQILVKTWEQVYISFFAIALGIAIAVPLGVVLTRF
                     PKVAKIIIAIASMLQTIPSLALLALMIPLFGIGKVPAIVALFIYSLLPILRNTYIGMN
                     NVNPTLKDCAKGMGMKPIQSIFQVELPLATPIIMAGIRLSTIYVIAWATLASYIGAGG
                     LGDLIFSGLNLFQPKLILGGTIPVIILSLIIDYLLGLLETALTPRTTRREA"
     gene            1886..2812
                     /locus_tag="VBC94_001771"
     CDS             1886..2812
                     /locus_tag="VBC94_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6980024.1"
                     /translation="MLKKSHFLQIFTLCLALLTISGCQLTDTKKPGHTTIKVAAQSST
                     ESSIMANIVTELIHHELGYNTTLISNLGSSTVTHQALLRGDADIAATRYTGTDITGTL
                     GLKAVKDPKEASKIVKTEFQKRYNQTWYPTYGFSDTYAFMVTKEFARQNKITKISDLK
                     KLSTTMKAGVDSSWMNREGDGYTDFAKTYGFEFSHIYPMQIGLVYDAVESNKMQSVLG
                     YSTDGRISSYDLEILRDDKKFFPPYEASMVVNNSIIKKDPKLKKLLHRLDGKINLKTM
                     QNLNYMVDDKLLEPSVVAKQFLEKNHYFRGDK"
     gene            2813..3463
                     /locus_tag="VBC94_001772"
     CDS             2813..3463
                     /locus_tag="VBC94_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6980025.1"
                     /translation="MKQMNTFQQFIYYFQHNGSYILEQFIHHFLISIYGVILAAIVGI
                     PLGIFIARRGKIKTFVMGIANVIQTIPSLAMISIIMLGLGLGIKTVVATVFLYSLLPI
                     ITNTYTGIRNVDSDLLDAAKGMGMTKRQRLFMVELPLSISVIMAGLRNALVVAIGITA
                     IGAFVGGGGLGDIIIRGTNATNGGAIILAGSLPTALMAIFSDLILGGIQRMLEPRK"
     gene            complement(3525..4706)
                     /locus_tag="VBC94_001773"
     CDS             complement(3525..4706)
                     /locus_tag="VBC94_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6980026.1"
                     /translation="MNINGIKLLSSRAVSKLGDVLYDYGNSTWIASIGGLGQKILGIY
                     QIVELLVSIVLNPFGGALADRFQRRKILLITDAICAIMCFLLSFIGDDKVMVYGLIVA
                     NAILAVSNAFSSPAYKSYIPEIVDKADIITYNANLETIVQIISVSSPVLGFLIFNNFG
                     IRITLIVDAITFLISFLFLYAIKVERVQLSKQEKVAIKNILADIADGFTYIKKEKEIM
                     FFLIIAALLNTFLAMFNYLLPFTNSLLKTSGAYATILSISAIGSIIGALIARKIKSSI
                     NSMLSMLVFSSLGVIVMGFPSLFELPIWISYSGSFLFNSLLTMFNIHFFSQVQIRVDE
                     AYMGRVMSTIFTIAIMFMPIGTLFMTIFSFALSNVSFIVIGCAIAILGGLGFSYSKKQ
                     F"
     gene            complement(4779..5632)
                     /locus_tag="VBC94_001774"
                     /pseudo
     CDS             complement(4779..5632)
                     /locus_tag="VBC94_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MutR family transcriptional regulator"
BASE COUNT         2057 a         1077 c          964 g         1982 t
ORIGIN      
        1 ttaacgattc tttttaaaat cattaaattt taaaacaaat ttcagacata ttgccaaagt
       61 tttgatatta ttactataat atagtttgta gaggagaata atatgggcca agaacctatc
      121 atcgaatatc aaaatatcaa taaagtgtat ggggaaaatg ttgcggttga agatattaac
      181 cttaaaattt accctggtga tttcgtttgt ttcatcggta cgagtggatc aggtaaaaca
      241 acattaatgc gtatggttaa ccatatgtta aaaccaacaa atggtactct attatttaag
      301 ggaaaagata tctctactat taaccccatt gaattaagac gcagaattgg atatgttatc
      361 caaaacattg gtttaatgcc tcatatgacc atttacgaaa atatagttct tgtaccaaaa
      421 ttattgaaat ggtcagaaga agctaaaaga gctaaagcaa gggaacttat taaattagtt
      481 gaattacccg aagaatattt ggatcgctac cctagtgagt tgtctggcgg tcagcaacaa
      541 cgtatcggtg tcattcgcgc tcttgcagca gaccaagata ttattttaat ggatgagcct
      601 tttggagctc tggatcctat tactagagaa ggtattcaag acttagtcaa gtctcttcag
      661 gaagaaatgg ggaaaactat catcttagtt actcatgata tggatgaagc cctcaagtta
      721 gcaacaaaaa ttattgttat ggacaatggt aaaatggtcc aagaagggac acccaatgat
      781 ctcttacatc atcctgctac tagtttcgtt gaacaaatga ttggagaaga gcgtctcctt
      841 catgcgcagg ctgatattac tcctgttaaa cagataatgt taaataatcc tgtttcaata
      901 actactgaaa aaacactaac tgaagctatt acactaatgc gccaaaaacg cgttgactca
      961 cttctagtaa ccgataacgg taaattaatt ggttttattg acttagaatc tctaagcagt
     1021 aaatataaga aagatcgact tgtttctgat atcttaaaac atactgattt ttatgttatg
     1081 gaagacgact tacttagaaa tactgctgag cgtattttaa aacgtggttt aaaatacgct
     1141 ccagttgttg accatgagaa taacttaaag ggcattgtta ctcgtgcatc cctagttgat
     1201 atgttatacg atattatttg gggcgatact gaaacggagg atcaataatg gtcaatttct
     1261 tatcacagta tggcatgcaa atattagtaa aaacatggga acaagtctat atctcatttt
     1321 ttgctatagc acttgggatt gcaattgctg ttccattggg tgtggtctta acacgttttc
     1381 ccaaagtagc caaaattatc attgctatag ctagcatgct acaaactata ccaagtttag
     1441 ccttattagc tttaatgatt ccattgtttg gaattggaaa agtcccagct atcgttgctt
     1501 tatttattta ttccttgctt cctatactaa ggaatactta tataggtatg aataatgtca
     1561 atccaacact taaagattgt gctaaaggga tgggaatgaa acctattcaa tctatatttc
     1621 aagttgagtt acctttagct actccaatta ttatggctgg tattcgcttg tctactattt
     1681 atgttattgc ttgggctaca ttagcttctt atataggtgc aggtggactt ggtgatctca
     1741 tttttagcgg gttaaatctt tttcagccca aattaattct agggggcaca attcctgtta
     1801 ttatcctgtc acttattata gattatcttt taggattact ggaaacagct ctaacgccca
     1861 gaacaacaag aagggaggct tagatatgct taaaaaatcg cactttttac agatatttac
     1921 actttgctta gccctcttaa cgatttctgg ttgtcaatta accgatacta aaaaacctgg
     1981 tcataccaca attaaggttg ctgcccaaag ttctacagag tctagtatca tggcaaatat
     2041 tgtcaccgaa ttaattcatc acgaattagg atacaacaca actttaataa gcaatcttgg
     2101 ttcctctacg gttactcacc aagctttgct ccgtggtgat gctgacattg ctgccacacg
     2161 ttatacagga acagacatca caggaactct tggcttaaaa gctgttaaag accctaaaga
     2221 agcttctaag attgtaaaaa ctgaattcca aaaacgctac aatcaaactt ggtatcctac
     2281 ttatggtttt tctgatactt atgcattcat ggttactaaa gagtttgcca gacagaataa
     2341 aatcaccaag atctctgatc tcaaaaagtt atcaacaact atgaaggcag gggttgatag
     2401 ttcatggatg aatcgcgagg gagatggata cactgatttc gctaaaacat acggttttga
     2461 attttcacat atttacccta tgcaaattgg cttagtctat gatgcggttg aaagtaacaa
     2521 aatgcaatct gtattaggct actccactga cggtcgtatt tcgagctatg atttagaaat
     2581 tttaagggat gataaaaaat tctttcctcc ttatgaagcc tctatggttg tcaacaattc
     2641 tatcatcaaa aaagatccta aactaaaaaa attactccat cgactcgatg gtaaaatcaa
     2701 tttaaaaacg atgcaaaacc ttaattatat ggtagatgat aaacttttag aaccttcagt
     2761 tgttgccaaa caatttttag aaaaaaacca ttattttaga ggagataaat aaatgaaaca
     2821 aatgaatact ttccaacaat ttatctacta cttccaacat aatggtagtt atatcttaga
     2881 acaatttatc caccactttt taatttcaat ttacggcgtt attttagctg caattgttgg
     2941 tatccctcta ggtatcttca tcgctagacg tggtaaaatt aaaacctttg ttatgggaat
     3001 cgcaaatgtc atccaaacca ttccttcttt agctatgatc tctatcatta tgttaggtct
     3061 agggctaggt attaaaacag tagttgcaac tgtctttctt tattctcttc tgccaatcat
     3121 cacaaatact tacactggta ttagaaatgt tgactccgac ttactagatg ctgctaaagg
     3181 aatgggcatg actaagcgtc aacgtttatt tatggttgag ttaccactat ctatttctgt
     3241 tatcatggct ggacttagaa atgcccttgt tgtagcaatt ggtattacag ccattggtgc
     3301 tttcgtcggt ggaggcggac ttggagatat catcataaga ggaactaatg caacaaatgg
     3361 tggtgctatt atcttagcag gatctcttcc aacagccctt atggcaattt tctctgattt
     3421 aatcctaggc ggtatccaac gcatgcttga accaagaaaa taactattaa aaacttgtca
     3481 gtagagacaa gtttttttga tgtttcagaa taatttctta agttctaaaa ttgtttttta
     3541 ctatagctaa atcctaatcc tcccaatata gcaatggcac atcctataac aataaagcta
     3601 acatttgata aggcaaatga aaagatagtc ataaatagtg ttcctatagg cataaacata
     3661 atagcaatag taaatatagt cgacatcaca cgccccatat acgcttcatc gaccctgatt
     3721 tgtacctgac tgaaaaaatg aatattaaac attgtcagaa gactattaaa aagaaaacta
     3781 ccactgtaag atatccatat cggtaattca aatagagatg ggaatcccat aactatgact
     3841 cccaaactac tgaatactag catagatagc atagaattaa tactagattt aatttttcta
     3901 gcaataagcg ctccaataat tgatccgata gctgatatac taagtattgt tgcataagca
     3961 cctgaagttt tcaataggga gttagtaaaa ggtaaaagat aattgaacat tgctaggaaa
     4021 gtgtttaata aagcagcaat aattagaaaa aacataattt ctttttcttt tttgatgtaa
     4081 gtaaatccat ctgcaatatc agctaaaata ttttttatcg ctaccttttc ttgttttgaa
     4141 agctgtaccc tttccacttt aatagcataa agaaacagga aagagatcaa aaaagtaatc
     4201 gcatcaacaa ttaaagtaat cctaatacca aaattattaa aaataaggaa acctaaaaca
     4261 ggggaactta cactaataat ttgcacaatg gtttccagat tagcattata ggtaatgata
     4321 tcagctttat ctactatttc aggaatataa gatttataag ccggagaaga aaaagcattg
     4381 gaaaccgcta agatagcatt agcaactatt agaccataca ccattacttt atcatctcca
     4441 ataaaagaaa gtaagaaaca catgatagca cagatagcat ctgttattag taaaattttt
     4501 cttcgttgaa agcgatcagc taaggcgcct ccaaaagggt tgaggacaat agaaaccaac
     4561 aactctacta tctgataaat acctaaaatc ttttgtccta gaccacctat cgaagcaatc
     4621 caagtactat ttccataatc ataaagcaca tcaccaagtt tactgactgc tcgactagat
     4681 agtaatttta taccattaat attcataatt ccctccttag ttcgacatta tagcaaacca
     4741 aaaggagaaa tattaaaaaa tcccataaat gggatttttt ataatcttgt taccctatta
     4801 aagtgttcct gaaaattttc aacatgatgt ttagaaccaa ttgcttggaa gcaaaaaata
     4861 gcttgcttca tgctttgtat tcctcgacta tcccctttag cataggcaag aaatcctttt
     4921 tcatagagta aaataatacg gtgataagca ttccgctcat tatttaaaag tgcttcaact
     4981 ttctctagaa aaaacgttgc ttcttctaga gcgcgccgtt ctattagtgt ctctaccaca
     5041 tttaggaggg ttgcttcgac aacattgcgg tttgtataaa tatctcgata atattccttt
     5101 tgattgataa cctctttaac aagttggcaa atatgccttg tgttgtaata ccggtaaaga
     5161 tttccaatca aaatatagtc actaatttcc caactatcct tttgaaagag gtaatccgac
     5221 aaaaagcaaa ggtcttcttc cgacattttt cttgaggagt catattcaca tatcattcct
     5281 ctaaaaagaa ctatgtttaa acgacaacgt atatctgaac tatccttttt tagtttacct
     5341 tcttccacag aaatcatctt ttctaaccca gctacgtctc tttgataatg atattgagcc
     5401 atctgagaca ttactgatca gatttttgat atttatttac tctatccata aattcgctaa
     5461 ttgataaatc aattgcctct aaagccccta aaaattttgt taatgataaa tctgattcac
     5521 ccctctcaaa tcgtgataac tgagaaacag aaagttcatt tgaagcaacc tgtttaagag
     5581 aaaactgtcg attaagtcga aattccctaa aaatagggcc atatataaac attagtattc
     5641 ctttctaacc gtaatctcat tggtaaactt atgaggtaat ttcaataatt tatatttacc
     5701 aactttaacc aatatatgag ctgatatata gtcattttct tttatttgta atatcatatc
     5761 acaagttctt ctaaatcaac atatgttaat ttatctggga atttttctcg taaggttact
     5821 ttcatatatt gagtacttac catacaaaat tcctccatca tatactcttt ttttcaatta
     5881 taaattaaaa tagaatcatc tcatctaact ttgtcattgc ctttgtgcta tttaaagtag
     5941 ttatttaaat ttacaatggt ttctcaagta tttagaagtt ttaaaaatga ctttaggaca
     6001 atttttgaat attacacaaa aaacacccca tggtgtgaac catgaagtgc ttccaatcgc
     6061 tgatatatag gtatatatta
//
LOCUS       DATROQ010000100         6075 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.101, whole genome shotgun sequence.
ACCESSION   DATROQ010000100 DATROQ010000000
VERSION     DATROQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.101"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            86..2125
                     /locus_tag="VBC94_001775"
     CDS             86..2125
                     /locus_tag="VBC94_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6980027.1"
                     /translation="MKRKYFVLNTVTVLTLATAMNTSSIYANSTETSASVAPTTNTIV
                     QTNDSNPTAKFASESGQSVIGQVKPANSAALTTVDTPHISAPDALKTTQSSPVVESPS
                     TKLTEETYKQKDGQDLANMVRSGQVTSEELVNMAYDIIAKENPSLNAVITTRRQEAIE
                     EARKLKDTNQPFLGVPLLVKGLGHSIKGGETNNGLIYADGKISTFDSSYVKKYKDLGF
                     IILGQTNFPEYGWRNITDSKLYGPTHNPWNLAHNAGGSSGGSAAAIASGMTPIASGSD
                     AGGSIRIPSSWTGLVGLKPTRGLVSNEKPDSYSTAVHFPLTKSSRDAETLLTYLKKSD
                     QTLVSVNDLKSLPIAYTLKSPMGTEVSQDAKNAIMDNVTFLRKQGFKVTEIDLPIDGR
                     ALMRDYSTLAIGMGGAFSTIEKDLKKHGFTKEDVDPITWAVHVIYQNSDKAELKKSIV
                     EAQKHMDDYRKAMEKLHKQFPIFLSPTTASLAPLNTDPYVTEKDKRAIYNMENLSQEE
                     RIALFNRQWEPMLRRTPFTPIANMTGLPAISIPTYLSESGLPIGMMLMAGANYDMVLI
                     KFATFFEKHHGFNVKWQRIIDKEVKPSADLIQPTNSLFKAHSSLVNLEENSQVTQVSI
                     SKKWMKSSVKNKPSVMAYQKALPKTGDTESSLSPVLVVTLLLACFSFVTKKNQKS"
     gene            2235..2813
                     /locus_tag="VBC94_001776"
     CDS             2235..2813
                     /locus_tag="VBC94_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6980028.1"
                     /translation="MKVKNKILTMVALTVLTCATYSSIGYADTSDKNTDTSVVTTTLS
                     EEKRSDELDQSSTGSSSENESSSSSEPETNPSTNPPTTEPSQPSPSEENKPDGSTKTE
                     IGNNKDISSGTKVLISEDSIKNFSKASSDQEEVDRDESSSSKANDEKKGHSKPKKELP
                     KTGDSHSDTVIASTGGIILLSLSLYNKKMKLY"
     gene            complement(2847..4088)
                     /locus_tag="VBC94_001777"
     CDS             complement(2847..4088)
                     /locus_tag="VBC94_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEO6980029.1"
                     /translation="MVLQDFDNLLKKYAQLIISKGLNVQKGHTLALTIDVEQVHLARL
                     LTEVAYEKGASEVIVDYTDDFITRQRLLHASDEVLTNVPQYTVDKSLALLNKKASRLV
                     VKSSNPNAFATVDPKRLSKTTRATAIALEEQSRAIQANKVSWNVAAAAGREWAALVFP
                     ELKTSDQQVDALWDTIFKLNRIYEDNPIAAWDAHEAKLLEKATRLNQEQFDALHYTAP
                     GTDLTLGMPKNHIWEAAGSLNAQGETFIANMPTEEIFSAPDYRRADGYVTSTKPLSYA
                     GVIIENMTFTFKDGKIINVTAEKGQETIQRLIEENDGARSLGEVALVPHKTPISLSGL
                     IFFNTLFDENASNHLAIGSAYAFNVEGGTEMTSQELDEAGLNRSSTHVDFMIGSEQMD
                     IDGIRADGTAVPIFRNGEWAI"
     gene            4266..4394
                     /locus_tag="VBC94_001778"
     CDS             4266..4394
                     /locus_tag="VBC94_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEO6980030.1"
                     /translation="MAFGDNGQRKKTGFEKLTLFVVILMVLVTVGGLVFGAISAIM"
     gene            4455..5768
                     /gene="obgE"
                     /locus_tag="VBC94_001779"
     CDS             4455..5768
                     /gene="obgE"
                     /locus_tag="VBC94_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEO6980031.1"
                     /translation="MSMFLDTAKISVKAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFKVNEGLRTLMDFRYNRNFKAKAGEKGMTKGMHGRGAEDLIVSLPPGTTVRDANT
                     GKVITDLVEHDQEFVVARGGRGGRGNIRFATPRNPAPEIAENGEPGEERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVVSAAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYDDYISINNELETYNLRLME
                     RPQIIVANKMDMPDSEENLAAFKEKLAANYDEFDDMPMIFPISSLAHQGLENLMDATA
                     KLLANTEEFLLYDETDMQEDEAYYGFNEDERPFEITRDDDATWVLYGDKLEKLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDIVRIGNFEFEFVD"
     gene            5794..5952
                     /locus_tag="VBC94_001780"
     CDS             5794..5952
                     /locus_tag="VBC94_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980032.1"
                     /translation="MGDKPISFRDKDGNFVSAADVWNAEKLEELFNTLNPNRKLRLER
                     EKLAKEKG"
BASE COUNT         2013 a         1009 c         1213 g         1840 t
ORIGIN      
        1 atataaaata acagttaata ttttagcaat ttgataacac ttagattata atgtaaacgt
       61 atactttttt agattggaag atgaaatgaa acgtaaatac tttgttctta atacggtgac
      121 ggttttaacg ttagctactg caatgaatac tagcagtatc tatgctaata gtactgagac
      181 aagtgcttca gtagctccta ctacaaatac tatcgttcaa actaatgaca gtaatcctac
      241 cgcaaaattt gcatcagaat caggacaatc tgtaataggt caagtaaaac cagctaattc
      301 tgcggcgctt acaacagttg acacgcctca tatttcagct ccagatgctt taaaaacaac
      361 tcaatcaagt cctgtcgttg agagtccttc tactaagtta actgaagaga catacaaaca
      421 aaaagatggt caagatttag ccaacatggt gagaagtggt caagttacta gtgaggaact
      481 cgtcaatatg gcatacgata ttatcgctaa agaaaaccca tctttaaatg cagtcattac
      541 tactagacgc caagaagcca ttgaagaggc tagaaaactt aaagatacta atcagccgtt
      601 tttaggtgtt cccttgttag tcaaggggtt agggcacagt attaaaggtg gtgaaaccaa
      661 taatggcttg atctatgcag atggaaaaat tagcacattt gacagtagct atgtcaaaaa
      721 atataaagat ttaggattta ttattttagg acaaacgaat tttccagagt atgggtggcg
      781 taatataaca gactctaaat tatacggtcc aacgcataat ccttggaatc ttgctcataa
      841 cgctggtggc tcttctggtg gaagtgcagc agctattgct agcggaatga cgccaattgc
      901 tagcggcagt gatgctggtg gttctatccg tattccatct tcttggacgg gcttagtagg
      961 tttaaaacca acaagaggat tggtgagtaa tgaaaagcca gattcgtata gtacagcagt
     1021 tcattttcca ttaactaagt catctagaga cgcagaaaca ttgttaactt acctaaagaa
     1081 aagcgatcaa acgctagtat cagttaatga tttaaaatct ttaccaattg cttatacttt
     1141 gaaatcacca atgggaacag aagttagtca agatgctaaa aatgctatta tggacaacgt
     1201 cacattctta agaaaacaag gattcaaagt gacagagata gatttaccaa ttgatggtag
     1261 agcattaatg cgtgattatt caaccttggc tattggcatg ggaggagctt tttcaacaat
     1321 tgaaaaagac ttaaaaaaac atggttttac taaagaagac gttgatccca ttacttgggc
     1381 agttcatgtt atttatcaaa attcagataa ggctgaactt aagaaatcta ttgtggaagc
     1441 ccaaaaacat atggatgatt atcgtaaggc aatggagaag cttcacaagc aatttcctat
     1501 tttcttatcg ccaacgaccg caagtttagc ccctctaaat acagatccat atgtaacaga
     1561 gaaagataaa agagcgattt ataatatgga aaacttgagc caagaagaaa gaattgctct
     1621 ctttaatcgc cagtgggagc ctatgttgcg tagaacacct tttacaccaa ttgctaatat
     1681 gacaggactc ccagctatca gtatcccgac ttacttatct gagtctggtt tacccatagg
     1741 gatgatgtta atggcaggtg caaactatga tatggtatta attaaatttg caactttctt
     1801 tgaaaaacat catggtttta atgttaaatg gcaaagaata atagataaag aagtgaaacc
     1861 atctgctgac ctaatacagc ctactaactc cctctttaaa gctcattcat cattagtaaa
     1921 tttagaagaa aattcacaag ttactcaagt atctatctct aaaaaatgga tgaaatcgtc
     1981 tgttaaaaat aaaccatccg taatggcata tcaaaaagca cttcctaaaa caggtgatac
     2041 agaatcaagt ctatctccag ttttagtagt aaccctttta ttagcttgtt ttagctttgt
     2101 aacaaaaaag aatcagaaaa gttgattgta atgtaaaata attcctattt ttcacttttt
     2161 agtatcatat tttcgatttt tatgttgtaa taaattataa tgaagatgta aggaattata
     2221 aaataggaaa taatatgaaa gtgaaaaata agattttaac gatggtagca cttactgtct
     2281 taacatgtgc tacttattca tcaatcggtt atgctgatac aagtgataag aatactgaca
     2341 cgagtgtcgt gactacgacc ttatctgagg agaaaagatc agatgaacta gaccagtcta
     2401 gtactggttc ttcttctgaa aatgaatcaa gttcatcaag tgaaccagaa acaaatccct
     2461 caactaatcc acctacaaca gaaccatcgc aaccctcacc tagtgaagag aacaagcctg
     2521 atgggagcac gaagacagaa attggcaata ataaggatat ttctagtgga acaaaagtat
     2581 taatttcaga agatagtatt aagaatttta gtaaagcaag tagtgatcaa gaagaagtgg
     2641 atcgcgatga atcatcatct tcaaaagcaa atgatgagaa aaaaggccac agtaagccta
     2701 aaaaggaact tcctaaaaca ggagatagcc actcagatac tgtaatagca tctacgggag
     2761 ggattattct gttatcatta agtctttaca ataagaaaat gaaactttat taaatataaa
     2821 aaggaggact aacctccttt ttatatttaa atagcccatt cgccatttct aaagattggg
     2881 acagcagttc catctgcacg aataccatca atatccattt gttctgaacc aatcataaaa
     2941 tcaacatgtg ttgaagaacg atttaaacca gcttcatcca attcttgact tgtcatttct
     3001 gttcctcctt ctacattgaa ggcatatgca gatccaatag cgaggtgatt agaggcattt
     3061 tcatcgaata aagtattaaa gaaaattagt ccagacagtg aaattggtgt tttatgtggg
     3121 acaagtgcaa cttccccaag cgatcttgcc ccatcatttt cctcgatcaa gcgttggatt
     3181 gtttcttgcc ctttttctgc agtgacattg ataattttac cgtctttaaa agtaaacgtc
     3241 atattttcga taataacgcc agcataactg agaggttttg tacttgtcac atacccatct
     3301 gcacgacggt aatcaggtgc tgaaaagatt tcttcagtag gcatattagc gataaaagtc
     3361 tctccctgag cgttgagact accggctgcc tcccaaatgt gatttttagg cataccaagc
     3421 gttaaatctg tacctggtgc ggtataatga agagcatcaa attgttcttg atttagtcta
     3481 gtagcttttt ctaataattt agcttcatgt gcatcccaag cagcaatagg attatcttca
     3541 taaatacgat ttaatttgaa aatggtatcc caaagagcat caacttgttg gtcgcttgtt
     3601 tttaattctg ggaagacaag tgcagcccat tctctaccag cagctgcagc cacgttccaa
     3661 gatactttat tagcttgtat tgctctactt tgttcctcta aggcaatagc ggttgctcta
     3721 gttgttttag ataaacgttt aggatcaaca gtagcgaaag cgttagggtt agaagatttc
     3781 acaactaatc gactagcctt cttatttaat agcgctaaag atttatcaac ggtatactgt
     3841 ggaacattcg tgagaacttc gtctgaagca tgaagtagtc gctggcgcgt gataaaatca
     3901 tctgtataat caacaataac ttcacttgct cccttttcat aagcgacttc agttaaaagc
     3961 cttgctaagt ggacttgttc cacatcgatt gttaaagcga gagtgtgccc tttttggaca
     4021 tttaaacctt tagaaataat taattgggca tattttttta aaaggttgtc gaaatcttgt
     4081 aaaaccatac tagctccttt aaatttacta ttttttataa tttatcataa actcttataa
     4141 agcacaagga aatatggaag cgatttaata aagatatatt taagctaacg gtttgtagtt
     4201 tgcgaaataa gacgtttcgt gttaatatag acttaactat atattcagta aatgaggtga
     4261 catgagtggc ttttggagat aacggacaac gaaaaaaaac tgggtttgaa aaactaactc
     4321 tttttgttgt tattttgatg gtactagtaa cagttggtgg tttggtattt ggtgctatta
     4381 gtgcaatcat gtaatgagaa acgctgtaaa gcgttttttt atactgaata gaataaagaa
     4441 taggaatttt agaaatgagt atgtttttag atactgccaa aattagtgtt aaggcaggtc
     4501 gcggtggtga tggcatggtt gcttttcgcc gtgagaaata tgtccccaat ggaggcccgt
     4561 ggggcggtga tggcggtaaa ggcggatcgg tcatttttaa agtaaacgaa ggtttacgaa
     4621 ctttgatgga ttttcgatat aaccgtaatt ttaaagcaaa agctggtgaa aaagggatga
     4681 ctaaaggtat gcacggtcga ggtgcagaag accttattgt gtcattacca cctggaacaa
     4741 ctgttcgtga tgctaataca ggtaaggtta ttaccgacct tgtggagcat gatcaggaat
     4801 tcgttgtagc cagaggtggc cgcggtggtc gtggaaatat tcgctttgca accccacgta
     4861 atccagctcc tgaaattgct gaaaatgggg agcctggcga agaacgtgaa ttacaattag
     4921 aattaaaaat tttggctgat gttggtctcg ttggtttccc atcagttggg aagtcgacgc
     4981 tacttagtgt tgtttcagca gcaaagccta aaattggtgc ctatcatttt acgacaattg
     5041 tccctaattt agggatggtt cgtacaaaat caggtgatag ctttgcaatg gctgatctcc
     5101 ctggtttgat tgaaggggct agtcaaggtg tcggattagg aactcagttc cttagacata
     5161 ttgagcgtac acgtgttatt ctacatgtta ttgatatgtc ggcaagtgaa ggacgagacc
     5221 cttacgatga ctatatttca attaataatg agttggaaac ttacaatctt cgattgatgg
     5281 aacgtccgca aattattgtt gccaataaaa tggatatgcc tgattcagaa gagaatttgg
     5341 cagcattcaa agaaaaatta gctgcaaact atgatgagtt tgatgacatg cctatgattt
     5401 ttccaatttc aagtttagca catcaaggac tagagaacct tatggatgcg acggctaaat
     5461 tattagccaa tacagaagag tttcttcttt atgacgaaac tgacatgcaa gaagatgaag
     5521 catactatgg atttaacgaa gatgaacgtc catttgagat tacacgtgat gatgatgcaa
     5581 cgtgggtatt gtatggtgat aagctagaaa aactctttgt tatgacaaat atggaacgcg
     5641 atgaatcaat aatgaaattt gctcgtcagt tacgtggtat gggcgttgat gaagcacttc
     5701 gagagcgcgg agctaaggat ggagatattg ttcgtatagg taacttcgaa tttgaatttg
     5761 tagattaatg cagtacttga ttggaggttt tttatgggag ataaaccgat atcatttcgt
     5821 gataaagatg ggaactttgt ttcagcggcc gatgtctgga atgctgaaaa attagaagaa
     5881 ctctttaaca cattaaatcc taatcgtaaa cttcgtttag aacgcgaaaa attagcaaaa
     5941 gaaaaaggat agttaagtaa ctatcctttt tagttatttt gggtatgaaa aaagactcta
     6001 aaacataatt gatagagtgt taaggtacag attactttat tattttaaac gtttttgagc
     6061 tttttgatat actga
//
LOCUS       DATROQ010000101         6047 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.102, whole genome shotgun sequence.
ACCESSION   DATROQ010000101 DATROQ010000000
VERSION     DATROQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.102"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(170..694)
                     /locus_tag="VBC94_001781"
     CDS             complement(170..694)
                     /locus_tag="VBC94_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO6980033.1"
                     /translation="MVKDRQIQKTKVAIYNAFISLLQENDYSKITVQDVIGLANVGRS
                     TFYSHYESKEVLLKELCEDLFHHLFKQGRDVTFEEYLVHILKHFEQNQDSIATLLLSD
                     DPYFLLRFRSELEHDVYPRLREEYITKVDIPEDFLKQFLLSSFIETLKWWLHQRQKMT
                     VEDLLKYYLTMVER"
     gene            826..1689
                     /locus_tag="VBC94_001782"
     CDS             826..1689
                     /locus_tag="VBC94_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO6980034.1"
                     /translation="MTSNKKVAIAFILNISFSVLEFIFGSLFFSGAILADAVHDFGDA
                     IAIGISATLEKKSKKDEDTIFSLGYKRFSLLGALITSLILISGSILVMIENIPKLWHP
                     TPVNYHGMFILAVIAIIINGLASFILHSGQSKHEEILSLHFLEDILGWLAIIVISLIL
                     NWKPLYILDPLLSVAISTFILSKALPKLLSTLKLFLDGVPDSIDYAALHDELKGLSQV
                     RSINQLNIWSMDGIDNRAIIHCCLNQLISEKDCKRAIRTICQHYKINDVTVEIDYSLR
                     EHQNHCKPLKN"
     gene            1774..2628
                     /locus_tag="VBC94_001783"
     CDS             1774..2628
                     /locus_tag="VBC94_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6980035.1"
                     /translation="MTDTMILNDNHKIPTLGLGTWMIDNDRVAQVVKDAIELGYRHID
                     TAQAYNNEKGVGVGIKESGITRESLFVTTKIAAEHKDYESATKSIDRSLEALGLDYID
                     LMIIHSPQPWKEWRETDKHFDQGNLEAWRALEDAQKSGKVKSIGVSNFLEKDLENILK
                     NGYVKPAINQILAHIGNTPFDLIDYCQSKGIQVEAYSPIAHGQALKSDGIQKMAEKYG
                     VSVAQLCIQYLLQLNLIVLPKASSKEHLQSNLDFDFVISDEDMSILKSLMFDDYGEFS
                     NFPVFSGK"
     gene            2738..3765
                     /locus_tag="VBC94_001784"
                     /pseudo
     CDS             2738..3765
                     /locus_tag="VBC94_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            3781..4170
                     /locus_tag="VBC94_001785"
     CDS             3781..4170
                     /locus_tag="VBC94_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6980036.1"
                     /translation="MHIKEVSKKTGITSDTIRYYERINLIPPIHRSESGIREFTQNDI
                     DILEFVRYFRGAGVSVESLIDYIHLVEKGDSTIPARLEILKDKRNNLEDRLQGLQEAL
                     NRLNHKIDNYQKVLVPREQELFDPTTK"
     gene            4180..4575
                     /locus_tag="VBC94_001786"
     CDS             4180..4575
                     /locus_tag="VBC94_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO6980037.1"
                     /translation="MQETTKSIFPKGEKNPYGEFFIRQSYLAALAKSPDGNVSVGNVT
                     FEAGCRNNWHVHLDGYQILLVTEGSGWYQEENKKAVSLEPGNVIVTDKGVKHWHGAKK
                     DSEFAHIAITAGKSEFYEAVSLEEYSRLG"
     gene            4603..4920
                     /locus_tag="VBC94_001787"
                     /pseudo
     CDS             4603..4920
                     /locus_tag="VBC94_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660666.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            5007..5291
                     /locus_tag="VBC94_001788"
     CDS             5007..5291
                     /locus_tag="VBC94_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HEO6980038.1"
                     /translation="MLTIQEMKDTTFELQENFRRLNYPIKQVAKDLQLNISEVESLLS
                     LDVTYPGDVCMLRDYLEDMLKKEGKEVYPFSRLASHSANRWYPYETPWRY"
     gene            5530..6021
                     /locus_tag="VBC94_001789"
     CDS             5530..6021
                     /locus_tag="VBC94_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO6980039.1"
                     /translation="MLSFQIVEKPAMILAGVTLENVKSNQECPKLWEKLHKQYNPSKL
                     KEKGIQQAIGICKTQPDFRFDYSATYQVETSVQAPKGLEIIRIPSATYAVISVKGPMP
                     SSLQETWRKIIQGFFQENNLKPANSPNLEIYSSQHPQDTDYQMEIWLAIADITNNSKT
                     NLI"
BASE COUNT         2086 a          982 c         1062 g         1917 t
ORIGIN      
        1 atggtgacat caacaatccc aaagaaactc tttcatcaag gggacttttt tatctcccga
       61 attatactca tttaatctaa taatttcaac tcgctatatt agacaatcaa tccattcatc
      121 acctctgata acgctaatga aataagagaa tctcccaact ctctataatt tacctctcta
      181 ccatagttaa ataatatttt agtaaatctt caacagtcat tttctgtctt tgatgaagcc
      241 accactttaa ggtctcgata aaactggata aaagaaattg ttttaagaaa tcttcaggaa
      301 tgtccacctt tgtaatatac tcctcacgca atctagggta aacatcgtgt tctaattcac
      361 ttctaaagcg taataaaaaa tagggatcgt cagataataa taacgtagcg atactatctt
      421 gattttgttc aaaatgtttt aagatatgaa ctaaatactc ttcaaacgta acatctctac
      481 cctgcttaaa taagtggtga aaaaggtctt cacagagctc cttgagtaac acttctttgc
      541 tttcatagtg agagtaaaaa gttgatcttc ctacatttgc aagtccgatt acatcttgaa
      601 ccgttatttt agaataatca ttttcctgta gcaagcttat aaaagcatta taaatcgcta
      661 cttttgtttt ctgaatttgt ctatctttta ccatatagga cactttctaa gttttgttca
      721 gtattgaaca agtgttcttt tttgtttcta gcaattctct tttatctcct agataataga
      781 agaaagaatc acactacaat accagtataa caaaaggaga aaaatatgac atcaaacaaa
      841 aaagtggcta tcgcttttat attaaatatc agcttttccg tccttgaatt catctttgga
      901 agtctattct tctcaggagc tatcttagcc gatgctgttc atgattttgg tgatgcaata
      961 gctattggca tctcagctac tttagaaaaa aaatctaaga aagatgaaga tacgatattt
     1021 tcattaggtt ataaacgttt tagcctttta ggagctctta tcacaagtct aattcttata
     1081 agtggatcta tcttagtcat gatagaaaat attccaaaat tgtggcatcc tactcctgtt
     1141 aattatcacg gcatgtttat tctagctgtc atcgctatta tcattaatgg tctagctagt
     1201 tttattctcc attctggtca aagtaaacat gaggaaatat taagccttca ttttttggaa
     1261 gatattctag ggtggttagc tattattgtt atatctctta tcttaaattg gaaaccgttg
     1321 tatatccttg atccattatt atcagttgcc atatctactt tcatcttatc taaagcactt
     1381 cctaaattac tatcaaccct gaaacttttt ttagatggtg ttccagattc catagattac
     1441 gctgcattac atgatgaact taagggactt tcgcaagtaa gatctatcaa tcaactcaat
     1501 atctggtcaa tggatggtat tgataatcga gcaatcattc actgttgctt aaatcaattg
     1561 atatctgaaa aagattgtaa aagagctatt cgaacgattt gccaacatta taagataaac
     1621 gatgttactg ttgaaatcga ttattcttta agagaacatc aaaatcattg caaaccatta
     1681 aaaaactaac ctgagtgtta gttttttcct tgacttggag ttaactctaa gtgttaagat
     1741 taggtcaata ctaatgaaag aaggaaaata aagatgacag atacaatgat attaaatgac
     1801 aatcataaaa taccaacttt aggtttaggt acgtggatga tagataatga tagggtagct
     1861 caagttgtta aagatgctat cgaattaggc taccgccata ttgatactgc acaagcatac
     1921 aacaatgaaa aaggggtcgg tgtagggatt aaagaatctg gtattacacg tgaatcttta
     1981 tttgttacga ctaaaattgc cgctgaacat aaagattatg aaagtgctac aaaatcaatt
     2041 gatcgatcac tagaagcttt agggcttgat tatattgatt taatgattat tcatagtcca
     2101 cagccttgga aggaatggcg tgaaacagat aaacattttg accaaggaaa tttagaagct
     2161 tggagagctt tagaggatgc tcaaaaatct ggtaaggtaa aatccatcgg tgtctcaaac
     2221 tttttagaaa aagacttaga aaatatctta aaaaatggtt atgttaaacc tgctatcaat
     2281 caaatcttag ctcacatcgg aaatacacct ttcgacttga ttgattactg tcaaagtaaa
     2341 ggtattcaag ttgaagctta ttcaccaatt gcacatggac aagcattgaa atctgatggt
     2401 attcaaaaaa tggctgaaaa atatggggtt agtgttgctc aattatgtat tcaatattta
     2461 ctacaattaa acctgatcgt tttaccaaag gcttctagta aagaacattt acagtctaat
     2521 ctagactttg actttgtaat tagtgacgaa gatatgtcga tattaaaatc acttatgttt
     2581 gacgattatg gggaattttc taacttccct gtttttagtg gaaaatgaaa ccatatgcgg
     2641 atttttacag atgtttaatt ttcaaaattt ttaagttttt cttgacttga agttaactct
     2701 aagagttata atgaagttat agaaaagagg taaggttatg aaagtagcta catttattga
     2761 acctggcaaa atggttataa ctgatacacc aaaaccagtc attgaacaag agacagatgc
     2821 tgttatcaaa attgttagag cctgtgtttg tggttcagac ttgtggtggt atcgtggtat
     2881 ttcaaaacgt gaaagtggtt cttttgcagg tcatgaggct attggtatcg ttgaggaagt
     2941 tggtactaaa gtaactgacg tgtcaaaagg tgattttgtt attgttccct ttacacatgg
     3001 ctgtggccaa tgtccgtctt gtaaggctgg atttgatgga aattgtacaa atcatcaagc
     3061 tgaaaaaaat gtagatacca aggtcaatat ctacgttata ctaatgcaaa ctgggcattg
     3121 gttaaaattc ctggtcaacc ttctgactat gataatgaga cacttaactc tcttctcacc
     3181 ttatcagatg taatggcaac tggttatcat gctgcagaag ttaaagaagg tgatacggta
     3241 gttgtcatgg gagatggtgc tgttggactc tgtggtgtta ttgctgctaa aatgttaggt
     3301 gctaaccgta tcattgcaat gagtcgtcac aaagataggc aggaactagc attgactttt
     3361 ggtgcaacgg atattgtcga agaacggggt gacgaagccg ttaaacgtgt tttagattta
     3421 accaatcaag caggtgccga tgctatttta gaatgcgttg gtacagagca gtctgttgat
     3481 acagctaccc aaattgctag gcctggtgct gtcataggac gtgttggtat cccacaaaat
     3541 ccagacatga ataccaataa tttattctgg aaaaatatcg gccttagagg tggcattgct
     3601 tctgtaacaa catttgataa gtctgtcctt ttagatgctg ttctaactca taaaattaac
     3661 ccaggcttag tttttacaaa atcctttgta ttagatgata ttcaaaaggc ttatgaagca
     3721 atggataaac gtgacgctat caaatcctta gtgattgttg actgaatatc gatgttaaaa
     3781 atgcatatca aagaagttag taaaaagact ggcattacgt cagataccat taggtattat
     3841 gagagaatta atcttattcc ccctattcat cggagtgaat caggaattcg tgagttcaca
     3901 caaaacgata ttgatatttt agaatttgtc cgctatttta gaggcgcagg tgtttccgtt
     3961 gaaagtctca ttgattatat tcatttggtt gaaaaaggcg attccacaat cccagctagg
     4021 ctagaaattt taaaagataa acgcaacaac cttgaagata ggcttcaagg cctccaagaa
     4081 gcacttaatc gtctaaatca caaaatagac aattatcaaa aagttcttgt tcctagagaa
     4141 caagaacttt ttgatccaac aacgaaatga ggaaatctta tgcaagaaac aactaagtct
     4201 atctttccta agggtgaaaa aaatccatat ggtgaattct ttattaggca atcctatctc
     4261 gcagcattag caaaatctcc agatgggaat gtttcagttg gaaatgtaac ctttgaagct
     4321 ggctgtcgta ataattggca tgttcatttg gacggttatc aaatcctcct cgttacagaa
     4381 ggttcaggtt ggtaccaaga agagaataaa aaggcagttt cattagaacc aggtaacgtg
     4441 attgtaactg ataaaggcgt taaacattgg catggtgcta aaaaagattc tgaatttgca
     4501 catattgcga ttacagctgg taagagtgag ttttatgaag ctgttagtct tgaagaatat
     4561 tcaagactag gttaaattca agtaaggagg aatcaatcca ttatgaaaga aaaacaaaca
     4621 gccggacgtc gtcaattaga agaatttgca cctgaatttg ccagatataa tgatgatatt
     4681 ttatttggtg aggtttgggc aaaagaggat catttaacag ataaaacacg ttctattatt
     4741 actatatctg ccttaattag ctgaggaaat cttgaacagt tagagcatca tcttcaattt
     4801 gccaaacaaa acggagttac taaagaagaa attgctgaca ttatcactca cctcgctttt
     4861 tatgttggat ggccaaaagc ttggtcagcc tttaataaag ctaaggaaat ttggatataa
     4921 aataacagac atagcaaggg agatactttc ccaaaagcca aaaagaaaga tcaaccaatt
     4981 attaaaccaa ctattaggag aatgatatgc ttactattca agaaatgaaa gatacaacct
     5041 ttgaattaca agaaaatttt agaaggttaa attatcctat taaacaagtt gctaaagact
     5101 tgcaacttaa tatcagtgaa gttgaatctc ttttaagtct tgatgttact tatcctggag
     5161 atgtttgtat gctgagagat tatctagaag atatgttgaa aaaagagggt aaagaagttt
     5221 atccattttc tagattagca tctcattcag caaatcgttg gtacccttat gagacaccat
     5281 ggcgttatta aagactaaag attaatccat catctttaga gaaataaaca aaaatagtca
     5341 atagattctt tttagaaaaa agctataaaa taagtgcctc ttagagaata ctctatatca
     5401 taagatttct tctttgaacc atatcattcc aaaagaaaat tatgattgct aacaagacaa
     5461 ttattctaaa aagaaactat tttaacgact ctataattac ttaaggaatc ataaagaata
     5521 aagattttta tgctatcatt tcaaattgtt gaaaagccag caatgatact tgctggagta
     5581 actttagaaa atgtaaagtc aaatcaagaa tgtcctaaac tttgggaaaa attacataaa
     5641 caatataatc catcaaaact aaaagaaaaa ggtatccagc aagccattgg catttgtaaa
     5701 acacaacctg attttcgttt cgattacagt gccacttatc aagtagagac aagtgtccaa
     5761 gccccaaaag ggttagaaat tattaggatc ccaagcgcta cctatgcagt catttctgtt
     5821 aaaggtccaa tgccatcgag tcttcaagaa acatggcgaa aaataataca aggatttttt
     5881 caagagaata atctaaaacc agctaactcg ccaaacttag aaatttattc atctcaacat
     5941 ccacaagata ctgattatca aatggaaatt tggcttgcta tcgcagatat tactaataac
     6001 tcgaaaacaa acttaatcta gtattgctta atataaaaaa gcccctt
//
LOCUS       DATROQ010000102         6001 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.103, whole genome shotgun sequence.
ACCESSION   DATROQ010000102 DATROQ010000000
VERSION     DATROQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.103"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            42..5367
                     /locus_tag="VBC94_001790"
                     /pseudo
     CDS             42..5367
                     /locus_tag="VBC94_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822652.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            5519..>6001
                     /locus_tag="VBC94_001791"
     CDS             5519..>6001
                     /locus_tag="VBC94_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO6980040.1"
                     /translation="MSRSQKVILTNMCLIEDNKGRIVMQIRDPKRYSWSGAALPGGHI
                     EEGESLYDALVREVREETGLTISNPKLVGMKHWYTDEGIRYLVFLYKTYDFSGELQSS
                     DEGDVIWIERSQLDKIELAYDMLNLLRVFDEDDISEMFYSKRLEDGFIRKILLNKKEL
                     G"
BASE COUNT         2182 a          942 c         1155 g         1722 t
ORIGIN      
        1 cttaacaaat aaaataatct aacttaaaaa tgagtggaat tatgaaaaga atttctttaa
       61 aaacaaccgg tgtactgatt gcgtttttag ctgtaaatgc ggtagcgacc tctgtagtgg
      121 tagatgcaga cacagtagtc cctcatacta gtgcgaatac tagtcagctt ttagaaaaaa
      181 acacaactat caagccttcc gaagttaaac agtcacgtac tgaaagtaat gcagtaaccc
      241 ctccaacaat agcctcgagt aactcacaaa agcttgagaa taaatcatct gtagattcaa
      301 atatactaaa gagtgaacaa cctgcacata aagttgctgg tgttaaacaa gtcgttcaag
      361 aacaaacgaa aaatcaaaaa gatgaaacct ctttacaggc taaaaagcaa gtacaatcaa
      421 gtgaaaagtt attaaatact tcatcacaac cttcaagtga ggaaaacaaa aacactaata
      481 agcctaataa ggctaatgaa gtggatatga aaaatgtcgt gaataatgct tcgacgaagc
      541 caactccccc atctgattct aaagtgtata agcgcgttat tgtaacattt gatcaagtaa
      601 ataaggaaaa agtagatgaa gcccttaaag caattccggg cactcgtatt aagttaactt
      661 atagtagcct ttttaatggc tattcattag cagtagatga aaaagaaatt gaaaaaattg
      721 gtaagattaa agagataaaa catattgaag cagttcaagt attaaaacct caaatgttta
      781 ctgctaacca atggacacat gtcttagctg cacgtttaaa agctaaacaa aatagtaaag
      841 ttgacggtag agggctactt attgcgacta ttgatagtgg tgtagatatc catcatcctg
      901 ccatgcgatt ggatgagaat gatccagatg tgatggcaaa gcgtcgtatt aaagatatta
      961 aggctcctta tacaaataaa gttccttttg catacaatta tcaacgaggc gataatttag
     1021 aattgaaaga cgacgttcag cgccctcatg gtatgcattt agcaggtatc ttagcgggaa
     1081 atgcaaagga tggttttaaa ggagttgccc ctaatgcaca gttattagtg tacaaaattt
     1141 caacaaatag agaagatcca tctgcagagt ggaccggctc tgatgcagtc ttccatgcaa
     1201 tggaagatgc cgctaaaaga ggggcagacg ttattagttt aagctttgga tatttgggaa
     1261 caggacgtcc aggtgaactt tggtatgaga caattaagaa ggtgactgac aagggtgtta
     1321 tcgtagtagc agcaatggga aactatggta attcggcttc atctactacc tacgataatg
     1381 tcgttgacaa ctctttggga acaaaagata ctgctgctat ggtgtctgtt gcagctgttg
     1441 ataaggttat tggagttggt tcagttcaaa atagtgctct tcttttagat gttttgaaag
     1501 ttggaaatct taaattagga tatgcagaac tgggacataa aagtcaaaca atattaaaaa
     1561 tgctcaaaaa taaaacattt gatctagtct atgttggtaa aggccagttg aaaaaagata
     1621 tctcagaaga gtaccttaaa agttatactg gaaaagttat cgtcgttgag cgtggaggag
     1681 agggcgttaa agataagcta aaacgtttta tcggaattgc taaaggggtt attatgataa
     1741 atgaacctgc ttattatagt tctgggaatt ttgatactca ccccatgttt gattatcact
     1801 atgatatacc agaaggtaaa tgggctatta gtctatcaaa aaaagatggt gatcagttga
     1861 ttgactacat caagaaaaac aaaactgcta aattagaatt tttacctaaa gaaacctttg
     1921 ttcgccgtcc aaatcctaca atttcaggct tttctagttg gggagtgaca ccagatttgc
     1981 atttgaagcc agatgtagtt gctccaggag aagatattta ttctactttt aacgacggta
     2041 cctatgggta catgtctgga acttcaatgg ctacccctca agtagctgcc attagtgcat
     2101 tagtaaaaag taaagtagct gaaataatgg cattaaaact tccaatgtta gctggtttga
     2161 caaacgttga tttgacaaag attgtcttga tgaatacaag tcaacctttg aaagatactc
     2221 taaataaagc tttagaaatt tcaccacgac gtcaaggtgc aggtatggtt aacttagaaa
     2281 aagctttagc taacgatgtt cttgtttttg gtgataaaac aggatcattc tctataaaag
     2341 agattaagaa ccataaaaga tttaccttga aattggtcaa tacaggacaa aaacgtcgta
     2401 actttagtat tgaaccagga gcgattttaa caactaaaat tgttgataaa gtgactaatc
     2461 aggggtatga aaatgtcgtt ttaaaggcag ttcacgctaa agtaatagag ggagcaactt
     2521 tacaaatggg taaagttatc tcactagcac cgcaatcagc catagagctt gatatagatc
     2581 ttgagataaa aaaacctata caagattttg ctgaagggta tttgtatttc aaaactttag
     2641 ataaagatgg acaagatatc tcagctcctt acatgggctt ctcaggaaca tgggattaag
     2701 aaccaatttt tgacgcacct atgtgggata gtcgttctaa aaataaatta acgaccttga
     2761 tgcaagcaac tttggttgga cacggaacta acaaatttta tgaaatcgga caagaaatag
     2821 gtagtaaaaa ggtcaatcca tccctattta ctatgtctaa tgtgcgtgta gctcccgata
     2881 aagttgtgcg tgtagcacct cgattttctt tattacgctc agcatcagat tttgacctct
     2941 ctattgtatc agaagcaaaa gacgattcac caatacttcg tcgtataaca gtgaaacatt
     3001 tcgtggagaa atattacaaa tcaagaaatg aggaagaaga ctcatattat gatgctttga
     3061 gaacgacaga atcatcgcta gtttgggatg gaaagtttta tgatcctaag actggtgatt
     3121 ttaaagctgc aaagccaggc ttatattact ttaagttacg tgcacgttca aatcctacag
     3181 atccttatca agtccaatac cttccaattt taatagataa tgaaagacca actttttctc
     3241 ttaaaaaagt taataacggc tttgatattt taacccatga taatcatcgt gttaaagaag
     3301 taattgttcg taataaagag acacaaaagc ctatcacagt taaaaaaatt gccgaaaatc
     3361 actatcatat tgatgagtta acgttaagag aatctgatct ggatataaaa gttgcagact
     3421 atgctgggaa ttcaaacaag gaaattgctt tatcagaatc agaagaagta tcagagaaat
     3481 cttactctag tgaggaagat aatacattac aaccacagtc actgtctcat cgcaatatga
     3541 aagctcaagc aaccagttca gatgatgacg atatctggga tgataacgat gatgacctta
     3601 acgaagaaaa agattttgat tttgaagaaa atatttctga tatcgaggat ggtttattat
     3661 taactcgaga aacggtatat tcaattcgaa ctgtaaataa aattcgaaat gttgaaaatg
     3721 aaagcttgga gcatagtgtt tctctcttat taaaagatgg ctatcgtgca gaaataagag
     3781 ttgttaacct tcatgagaac gctaaagatc caaaacattc cgacccatat cgtacagtat
     3841 ccaaccctat ttatatgtat ggtgatgact ctgacacatt taaaaccaca tgggtcaaat
     3901 ttatgtctgg gaataacatc atgaatttga aggtttataa taagaatgat aaattggtat
     3961 ttagtcgtgg ctatcatatt accgttgatg tacaacctcc aaaattagaa ctggacaaga
     4021 ctaacataga agaagctgct gacaaagata actttatcac tggtcaaatc tatgctcaaa
     4081 atggagaaat tgaaatcaaa gggcacgttt ctgataatgt aaaaggttgg ggtctttcta
     4141 ttaatggtga tagcgtcgat gttaaatatt caagtggtga atttatcagt aatagatgag
     4201 catttactca taagataaaa gcagcagcag gagatattgt tacgatttca gcggtagata
     4261 catcaggtaa taccgtttct gaacattata aggtagttaa tgatgtggat acaccgaagt
     4321 taactattaa tgaagcagat ttattatccg gaaaagtcaa agtggttata gctaaagcag
     4381 aagataatca ccttaaaaat ttaacgataa ctgttaatgg caagccctac acagaaggac
     4441 aaagtattga ctcttaccaa ttaaaaggtt tcgaagggcg ttacgtcatc aaagtatcag
     4501 cagttgataa atcaggtaaa gtcactgaaa aaattataac aatcggcaac ttccctgaat
     4561 tatcagcaag tttacgcaaa acctctttcc aaatgcaaga tcttacctct atcgataatt
     4621 tattatccct agcccacaat cagactgcgg aattgattaa gccagtccaa ctcatggaca
     4681 aaaaagtgag ctttacggca aaaatatctg acccccttgg tcgttctgtg gttagagagt
     4741 ttgaagcatt attatcaact tcaaacctta aagtagattc aaaactaaaa ccacatttga
     4801 aaaaacaaac tctcctttta acagatccaa aaggaattac ggaccttttg gatctgttaa
     4861 aaggatagct ctattttatt gacagaaata gtcgataata aagtgggaaa tcatactata
     4921 aagggtttga tatttaaaaa tggtgcatat aaccatttcg aaacaagtat acaaattgta
     4981 aaaacaatgg aagaaatcaa ctctgacaga cgtttgaaaa atgatcataa aagctctgac
     5041 gacacaaaac taaaaaaaga aacagagtcg ttaaaagggc ataaggttga tccagcagtt
     5101 catggtgaat ttaaagaaat atctaaacat caaatggata aaccacaagt gatagctgta
     5161 ggcatgaaag ggagtacaaa gacaccccca tttgttcata gtcaaatgga agcgaagaaa
     5221 acaactaaga ttccaatgtc tagagcggca aaacatgaat tgccaaggac aagtgatgac
     5281 aataataatt tactggctaa aggaattgca ctcatagtaa ttgtacttgg tttactaggc
     5341 cttaaaaaag cttcaaagaa atattgattt tgattaaatt agttttctaa tttattaagc
     5401 taaaattcct ttggataaat tattaaggga gttttaactt aatctattat tttgtagaaa
     5461 aattgataaa taaatggtat tgtaactcta tcactaaatt tatatactag aggaaataat
     5521 gtcacgcagt caaaaagtta ttttaactaa tatgtgtctt attgaagata ataaaggacg
     5581 tattgtgatg caaatccgtg atcctaaacg ttattcttgg tcaggagctg ctctaccagg
     5641 cggccatatt gaagaaggag aaagcttgta tgatgctctc gttcgagaag ttcgcgagga
     5701 gacaggttta acaataagta atcccaaatt agttggtatg aagcattggt acacagatga
     5761 aggcatccgt tatttggtat tcctttataa aacatatgat ttttcaggtg aattacaatc
     5821 gtcagatgaa ggtgatgtta tatggattga acgttcacaa cttgataaga ttgaactagc
     5881 atatgatatg cttaaccttt tgagagtatt tgacgaagat gacatctcag agatgtttta
     5941 tagtaagcga cttgaagatg gttttataag aaaaatttta ttaaacaaaa aagagctagg
     6001 a
//
LOCUS       DATROQ010000103         5969 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.104, whole genome shotgun sequence.
ACCESSION   DATROQ010000103 DATROQ010000000
VERSION     DATROQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.104"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            33..935
                     /locus_tag="VBC94_001792"
     CDS             33..935
                     /locus_tag="VBC94_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEO6980041.1"
                     /translation="MKIDDLRKSDNVEDRRSSSGGSFSSGGSGLPILQLLLMRGSWKT
                     KLVVLIILLLLGGGGLTSIFNDSSSPSSYQSQNVSRSVDNSATREQIDFVNKVLGSTE
                     DFWSQEFQTQGFGNYKEPKLVLYTNSIQTGCGIGESASEPFYCSADKKIYLDISFYNE
                     LSHKYGATGDFAMAYVIAHEVGHHIQTELGIMDKYNRMRHGLTKKEANALNVRLELQA
                     DYYAGVWAHYIRGKNLLEQGDFEEAMNAAHAVGDDTLQKETYGKLVPDSFTHGTAQQR
                     QRWFNKGFQYGDIQHGDTFSAEHL"
     gene            complement(1004..3409)
                     /locus_tag="VBC94_001793"
     CDS             complement(1004..3409)
                     /locus_tag="VBC94_001793"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEO6980042.1"
                     /translation="MLVSYKWLKELVDVDVTTAELAEKMSTTGIEVEGVETPAEGLSK
                     LVVGHIVSCEDVPDTHLHLCQVDTGGDELRQVVCGAPNVKTGINVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSESIIPKEFSDGIQILPEGAIPGDSIFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYGKKVHFEEKNLIEEAERAADKISVVIESDK
                     VLSYSARIVKNVTVAPSPQWLQNKLMNAGIRPINNVVDVTNYVLLTYGQPMHAFDFDK
                     FDGTTIVARNAENGEKLITLDGEERDLIADDLVIAVNDQPVALAGVMGGQSTEIDSSS
                     KTVVLEAAVFNGTSIRKTSGRLNLRSESSSRFEKGINYDTVSEAMDFAAAMLQELAGS
                     QVLSGQVTEGVLPTEPVEVSTTLGYVNTRLGTELTYTDIEEVFEKLGFAISGSEVKFT
                     VLVPRRRWDIAIQADLVEEIARIYGYEKLPTTLPEAGATAGELTSMQRLRRRVRTVAE
                     GVGLSEIITYALTTPEKAVQFSTQATNITELMWPMTVDRSALRQNVVSGMLDTIAYNV
                     ARKNSNLAVYEIGKVFEQTGNPKEDLPTEVETFTFALTGLVEEKDFQTKAKPVDFFYA
                     KGIVEALFIKLKLDVTFVAQKGLASMHPGRTATILLDGKEIGFVGQVHPQTAKQYDIP
                     ETYVAEINLSTIESQMNQALIFEDITKYPSVSRDIALLLAESVSHHDIVLAIESSGVK
                     RLTAIKLFDVYAGNNIAEGYKSMAYSLTFQNPNDNLTDEEVAKYMEKITKSLVEKVNA
                     EIR"
     gene            complement(3463..3983)
                     /locus_tag="VBC94_001794"
                     /pseudo
     CDS             complement(3463..3983)
                     /locus_tag="VBC94_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(4066..5106)
                     /gene="pheS"
                     /locus_tag="VBC94_001795"
     CDS             complement(4066..5106)
                     /gene="pheS"
                     /locus_tag="VBC94_001795"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEO6980043.1"
                     /translation="MDLQKQLEELKISTQEKLKEMTGNHTKELQDLRVQVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDILTKAFEEQAKVVEAAKIQAQLESESVDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQVVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTMDQHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQI
                     EGLVVGENISMGDLKGTLQLISQKMFGAERKIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKQTGWIEILGAGMVHPSVLEMSGIDSEKYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDVRFTDQF"
     gene            complement(5397..>5969)
                     /locus_tag="VBC94_001796"
     CDS             complement(5397..>5969)
                     /locus_tag="VBC94_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6980044.1"
                     /translation="KTDLNAKVSSAPYAINEIKKVNNQIVPTKANALLTKATRQYRNR
                     EETGNGRTYWKPAGWHQINGLKGSYNHAVDRGHLIGYALVGSLRGFDASTSNPKNIAT
                     QAAWANQANSNQSTGQNYYETLVRKALDRHKTVRYRVTLIYDRDNLLSSGSHIEAKSS
                     DGSLEFNVFIPNVQSGLLFDYATGKVKQTK"
BASE COUNT         1822 a         1247 c          977 g         1923 t
ORIGIN      
        1 taatgagcct ataaaacact ttggagatac ttatgaaaat agatgaccta agaaaaagcg
       61 acaatgttga agatcgtcgc tccagtagcg gaggttcatt ctctagcgga ggaagtggat
      121 taccgattct tcaactttta ttgatgcgag ggagttggaa aaccaagctt gtggttttaa
      181 tcatcttact gctacttggc ggagggggac taaccagcat ttttaatgac tcatcctcac
      241 cttctagtta ccaatctcag aatgtctcac gttctgttga taatagcgca acgagagaac
      301 aaatcgattt cgttaataaa gtccttggat caactgagga tttctggtca caagaattcc
      361 aaacccaagg ttttggaaat tataaggaac caaaacttgt tctttacacc aattcgattc
      421 aaacaggttg tggtataggt gaatctgctt cagaaccatt ttattgttca gcagataaaa
      481 aaatctatct tgatatttct ttttacaatg aattatcaca taaatatggt gctactggtg
      541 attttgctat ggcctacgtc atcgcccatg aagttggtca ccacattcaa acagagttag
      601 gcattatgga taagtataat agaatgcgac acggacttac taagaaagaa gcaaatgctt
      661 taaatgttcg gctagaactt caagcagatt attatgcagg ggtatgggct cactacatca
      721 ggggaaaaaa tctcttagaa caaggagact ttgaagaggc catgaatgct gcccacgccg
      781 tcggagacga tacccttcag aaagaaacct acggaaaatt agtgcctgat agctttaccc
      841 atggaacagc tcaacaacgc caacgctggt ttaacaaagg ctttcaatat ggtgacatcc
      901 aacacggtga tactttctcc gcagaacatc tataaatttt aaataaaaaa acagcatctt
      961 aattgatgct gttttttatt tatttttcaa cgtaagatat aaattatctt atttctgcat
     1021 taactttctc aacgagtgat ttagtgattt tttccatata tttagccact tcttcgtccg
     1081 ttagattgtc attagggttt tggaaagtta agctataagc cattgattta tacccttctg
     1141 caatattatt tccagcgtag acatcaaaca gtttaatcgc tgttagacgt tttacccctg
     1201 atgactcaat tgctaacaca atatcatgat gactaacaga ttctgcaagt aataatgcaa
     1261 tatcgcgaga tacagacgga tacttcgtaa tatcttcaaa aatcaacgct tggttcattt
     1321 gactttcaat agttgataga ttgatttcag caacataagt ttcaggaata tcatattgtt
     1381 ttgcagtttg tgggtgtact tgaccaacaa aaccaatctc tttcccatct aataagatag
     1441 ttgctgtacg acctgggtgc atacttgcta aacctttttg tgctacaaaa gtaacatcta
     1501 attttaattt aataaagagc gcctctacaa tacctttggc gtagaagaaa tcaactggct
     1561 tggcttttgt ttggaaatct ttttcctcta ctagacctgt taaggcaaaa gtaaacgtct
     1621 ctacttcagt tggtaaatct tcttttggat taccagtttg ttcaaaaact tttcctatct
     1681 cataaactgc aaggttactg tttttgcgag caacgttata agcaattgta tcaagcattc
     1741 ctgaaacaac attttggcga agtgctgagc gatcaacagt catcggccac ataagttcag
     1801 ttatattagt tgcttgagtt gagaactgca ctgctttttc tggagtcgtt aaagcatagg
     1861 tgataatttc agataatcct actccttcag caaccgtacg gactctacga cgtaagcgtt
     1921 gcatactcgt taattctcct gcagtcgctc cggcttctgg caatgttgtt ggtaattttt
     1981 cgtaaccata aatacgggca atctcttcaa ctaagtcagc ttgaatggca atatcccatc
     2041 tacgacgcgg taccaacaca gtaaatttca cttctgaacc agaaatagca aagccgagtt
     2101 tttcaaatac ttcttcaata tcagtatagg taagttctgt tcccaaacga gtattaacgt
     2161 aaccaagagt cgttgatacc tctaccggct cagttggaag aactccttct gtaacttgcc
     2221 cagaaagaac ttgactgcct gccaattctt gtaacattgc agccgcaaaa tccatagcct
     2281 ctgaaaccgt atcataattg ataccttttt caaagcgaga agatgactct gaacgaaggt
     2341 tgagtcttcc acttgttttg cggatacttg ttccattaaa tactgccgct tcaaggacca
     2401 ctgtcttaga agaactatca atttcagttg attgtcctcc cataacgcct gccaaagcaa
     2461 ctggttgatc atttaccgca atgaccaagt catcagctat taagtcacgc tcttcaccat
     2521 caagagtaat taacttttca ccattctcag cattacgtgc tactatagtt gtcccatcaa
     2581 atttatcaaa atcaaaagca tgcattggtt gaccataagt caaaagaaca tagtttgtca
     2641 catcaacaac attattaatt ggacgtatac cagcattcat cagtttattt tgaagccatt
     2701 gtggacttgg tgcaactgtt acattcttaa caatacgagc cgagtaagat aaaactttat
     2761 ctgattcaat gacaacagaa atcttatcag ctgctctttc tgcttcttca atgagatttt
     2821 tttcttcaaa atgaactttt ttcccataaa ttgctgctac ctcatgggca acaccacgca
     2881 ttgaaagagc atctgcacgg tttggtgtaa ttgataattc aataatttca tcatctaagt
     2941 caagataaga gaaaatacta tcaccaggta tagctccctc aggtaaaatt tggataccat
     3001 cagaaaactc ttttggaatg atactttccg ataagcccaa ctcttgaagt gaacaaatca
     3061 ttccaagtga ctccatacca cggattttac cttttttaat cttatagtta tctgcaatac
     3121 gagcaccagg aaccgctacg ataacattaa taccagtctt gacatttgga gcaccacaga
     3181 caacttgacg aagttcatcg ccccctgtat caacttgaca caaatgtaag tgggtatctg
     3241 gaacatcttc acacgaaaca atatgaccta caactaattt tgataaacct tccgctggtg
     3301 tctccacacc ttcaacctca ataccagtgg ttgacatttt ctcagctagt tctgctgttg
     3361 tgacatcaac atccactaac tcttttaacc atttatacga tactaacatg taatttctct
     3421 ctatttattt tcttttattt ttatacaagt gctttgtagt ttttagtgta aactttttct
     3481 taataaccaa tccgtatcca ctttatcacc tactgaaaat tggtgctcag aaaatttttc
     3541 aaatccgtat ttagcgtaaa atgcctgagc tttatgatta aactcccaaa cacctaacca
     3601 agcccaatct aagcctgatt tataagctaa atccaaagca aattcgaaag tagccttacc
     3661 tatacctttc ccttggtagg catctagaag ataaatcctt tgaatttcaa atgctttatc
     3721 aagttttttt cagtttgctg acttccccag tttactttta aataacccac taaatctgta
     3781 tcaagataaa caaaataagt atcagattca gcatgggtaa tttccgattt taatactggt
     3841 aaagtgtaag actcattgaa aaatgcttga agttgttcct cactattatc atgagaaaac
     3901 gtctgccgaa aggtttctat agcaagattc tgtaaaatat tcacattatc aaggccaacc
     3961 tttttaaaag taatttgaga cattagaaaa cctcctattg agtaaggtaa tagcgttaac
     4021 ataaatttat catcacacca atgatgctac ttgctatcta aaatcttaaa attgatctgt
     4081 gaagcgaaca tcaccttgat agaagccacg aatgtcattg ataccgtagc gtaacattgc
     4141 aatacgttct tgacctaagc caaaggcaaa accggagtat ttctcagaat caataccaga
     4201 catttccaaa acacttgggt gaaccatacc agcacccaat atctcaatcc aacctgtctg
     4261 tttgcagaca ttacatccct tacctccgca cttgaagcaa gagacatcaa cttcaactga
     4321 aggttctgtg aatgggaaat atgaaggacg aagtcggatt ttacgctctg ctccaaacat
     4381 tttttgagaa atcagttgaa gtgttccttt gagatcgccc attgaaatat tttctccaac
     4441 aactaagcct tcaatttgat ggaattgatg gctatgagta gcatcatcag tatcacgacg
     4501 gaaaacacgt ccaggagaaa tcatttttaa aggcccttta gaaaaatcat gttggtccat
     4561 tgttcgtgct tgaacaggcg aagtatgagt acgtagcaag atttcttctg taatataaaa
     4621 agtatcttgc atatcacggg ctggatgatc ttttggtaaa ttcattcgtt caaaattata
     4681 gtagtctttc tcaacttcaa aaccatcaac cacttggaat cccataccta aaaagatatc
     4741 ttcaatctct tcgctagttt gtgttaagac atgacggtga cctaatgtca tttgacgacc
     4801 aggcaaggta acgtcgacac tctcagactc taactgtgct tgaatcttcg cagcttccac
     4861 tactttagct tgttcctcaa acgcttttgt taaaatatca cgtacttcat tgacttgttt
     4921 tccaacaact ggtcgtaaat cattagataa atctttcaga ccttttagta attctgttag
     4981 agaacctttt tttcctaaaa cttgtacacg taagtcttgt aattctttag tgtggttccc
     5041 agtcatttct ttaagctttt cttgagttga tattttcaac tcttctaatt gtttttgtaa
     5101 atccatcttt cctccaagtc aaaataagta gctttgaaaa caaaaaaagc gtgccactcc
     5161 cccttaatcg gagcgttgac acgcggtacc atccgttttc tatctgatcg accaatcaga
     5221 ccttaattat agtcctattt gtgagtgaat tccaccctct ttcactgatt cagcttgcag
     5281 tcacggctga actccctaag cagcttccct tggtttacta gtctcacaga cctctattca
     5341 attaaggact attttatcaa atttttcagt ctttaacaag cactattaga taaagattac
     5401 tttgtctgct tcacttttcc tgttgcataa tcaaataata atcccgattg tacattagga
     5461 ataaaaacat taaattctaa acttccatct gaagatttag cctcaatatg tgatcccgaa
     5521 gataaaagat tatctctgtc ataaattaaa gtcacacgat acctaacagt tttatgacga
     5581 tccaatgctt tacgaactaa agtttcataa taattttgcc ctgttgattg gttgctatta
     5641 gcttggtttg cccaagctgc ttgtgtggca atatttttag gattacttgt ggaggcatca
     5701 aatcctctta aactccctac taaagcataa ccaattaaat ggcctctgtc cacagcgtgg
     5761 ttatagctcc cttttaatcc atttatctga tgccatccag ctggtttcca ataagttcta
     5821 ccattccctg tttcttctcg atttcgatat tgacgagttg cttttgttaa taaggcattt
     5881 gctttagttg gaacaatttg gttattaacc ttctttattt cattaatagc atatggtgca
     5941 cttgacactt tagcattcaa atctgtttt
//
LOCUS       DATROQ010000104         5965 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.105, whole genome shotgun sequence.
ACCESSION   DATROQ010000104 DATROQ010000000
VERSION     DATROQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.105"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(19..1800)
                     /gene="uvrC"
                     /locus_tag="VBC94_001797"
     CDS             complement(19..1800)
                     /gene="uvrC"
                     /locus_tag="VBC94_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEO6980045.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKNGTIIYVGKAKNLKNRVKSY
                     FHGSHNTKTELLVSEIEDFEYIVTTSNTEALLLEINLIQENMPKYNIRLKDDKSYPYI
                     KITNERYPRLMITRQVKKSDGTYFGPYPDSGAATEIKRLLDRLFPFKKCTNPANKVCF
                     YYHLGQCNAHTVCQTNKAYWDSLREDVKQFLNGKDNKIVNGLTEKMKSAAMTMEFERA
                     AEYRDLIEAISLLRTKQRVIHQDMKDRDVFGYFVDKGWMCVQVFFVRNGKLIQRDVNM
                     FPYYNEPEEDFLTYIGQFYQDTKHFLPKEVFIPQDIDAKSVETIVGCKIVKPQRGEKK
                     QLVNLAIKNARVSLQQKFDLLEKDIRKTHGAIENLGNLLNIPKPVRIEAFDNSNIQGT
                     SPVAAMVVFVNGNPSKKDYRKFKIKTVIGPDDYASMREVIHRRYSRVLKDGLTPPDLI
                     VIDGGQGQVNIARDVIENQLGLAIPIAGLQKNDKHQTHELLFGDPLEVVELPRNSEEF
                     FLLHRIQDEVHRFAITFHRQLRSKNSFSSKLDGITGLGPKRKQLLMKHFKSLPNIQKA
                     DIEDIIMCGIPRTVAESLRDSLNDPPK"
     gene            1959..2726
                     /locus_tag="VBC94_001798"
     CDS             1959..2726
                     /locus_tag="VBC94_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEO6980046.1"
                     /translation="MIDVENILEKMKPNQKINYDWVMQQMVKQWQASDIRPKILMHVC
                     CAPCSTYTLEYLSQWADVTIYFANSNIHPKDEYYRREYVTQKFVHDFNKNTGYSVQFL
                     SAPYEPNEFFKIVHGLEEEPEGGDRCKVCYDFRLDKTAEKAVELGFDYFGSALTISPH
                     KNSQTINTIGIDVQKIYDTQYLPSDLKKNKGYQRSVEMCKDYDIYRQCYCGCIFGAKD
                     QGINLLQIKKDAKAFVSDKDGKEEFPNIRFTFNGKSM"
     gene            complement(2785..4125)
                     /locus_tag="VBC94_001799"
     CDS             complement(2785..4125)
                     /locus_tag="VBC94_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO6980047.1"
                     /translation="MYQTQTNKEKFVLFLKLFIPVLIYQFANFSATFIDSVMTGQYSQ
                     LHLAGVSTASNLWTPFFALLVGMISALVPVVGQHLGRGNKEQIRTEFHQFLYLGLILS
                     LILFLIMQFIAQPVLGSLGLEDEVLAVGRGYLNYMLIGIMPLVLFSICRSFFDALGLT
                     RLSMYLMLLILPFNSFFNYMLIYGKFGMPRLGGAGAGLGTSLTYWAIFIVIIIVMSLH
                     PQIKTYHIWTLERIKAPLIIEDIRLGLPIGLQIFAEVAIFAVVGLFMAKFSSIIIAAH
                     QAAMNFSSLMYAFPLSISTALAITISFEVGAERFQDANTYSRIGRLTAVGITSGTLLF
                     LFLFRENVAAMYNSDPHFVAITAQFLTYSLFFQFADAYAAPVQGILRGYKDTTKPFMI
                     GAGSYWLCALPLAVILEKNSQLGPFAYWIGLITGIFVCGLFLNQRLQKIKKLYY"
     gene            complement(4225..4641)
                     /locus_tag="VBC94_001800"
     CDS             complement(4225..4641)
                     /locus_tag="VBC94_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6980048.1"
                     /translation="MVMKAIEIYMNINGRGAEAITFYEEAFNTKAEHVTTFAQGWPDC
                     PPELEHLIMNAQFNIDGIRMQLSDNNPIFDFQIGQNMTAALIVESVEEAQELFNKLSR
                     DAMNIKMPLQAVPWSPAYGNVTDKYGMTWQINTELS"
     gene            complement(4645..5142)
                     /locus_tag="VBC94_001801"
     CDS             complement(4645..5142)
                     /locus_tag="VBC94_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6980049.1"
                     /translation="MIIDFKTVSTKGLETSPVAEALAGLRANEARYFWNKFKVAYETF
                     PVSDYPELMADINHLLKNERNLVFAAKPLEISQLFLENRILWTHVFYEDGLAINILYE
                     LDNPKKRAVGLKLSDGMDIPIELADKFKFARQKSKLAGIIRGSYFTLKGENETLMQRW
                     LDNMK"
     gene            5364..5960
                     /locus_tag="VBC94_001802"
     CDS             5364..5960
                     /locus_tag="VBC94_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEO6980050.1"
                     /translation="MTKKIFLFICATLLVGIALYTPTTSIFNHNNVYAATKKAKIKFN
                     KTQKKIVKKAREYAKSGHMSKDSIIEKLKKDSKKYRQEDINFVINNLKVDYKKNALIS
                     AKIYSKTMNLSKQSIFEQLYSESPDKATHSDKFTKEESQYAIDHLKVDFKENALETAK
                     SYQSSSSLSKEEIYKQLTSTLGDKFTNDEAQYAVDHLK"
BASE COUNT         2060 a         1131 c          886 g         1888 t
ORIGIN      
        1 actgttctac taagtttttt atttaggagg atcattaaga gaatctcgga gactttctgc
       61 aactgtccta ggaataccac acataataat gtcctcaata tcagcttttt ggatattagg
      121 taagctttta aaatgtttca ttagcaattg tttacgtttt ggtcccaacc cagtgatacc
      181 atctaatttt gaagaaaagg aatttttgga gcgtaattga cgatgaaaag taatagcaaa
      241 acgatgtacc tcatcttgaa tacggtgaag taagaaaaat tcttctgaat tacgcggtaa
      301 ttctactact tctaaaggat ctccaaaaag taattcatgt gtttggtgtt tatcattctt
      361 ttgcaaacct gcaataggta tggctaaacc taattggttc tcaataacat cacgcgctat
      421 atttacttgc ccctgaccac catcaattac aatcaagtcc ggaggtgtca agccatcttt
      481 taaaacccta ctataacgcc gatgaatgac ttctctcata ctagcataat catctggtcc
      541 gataacagtc ttaattttaa actttcgata gtctttttta cttggatttc cattcacaaa
      601 taccaccata gcagctactg gacttgtccc ttgaatattg gaattatcaa atgcttctat
      661 gcgtactggt ttagggatat taagaagatt acctaaattt tcaattgccc catgagtttt
      721 acgaatatct ttttccagta agtcaaactt ctgttgtaaa ctgacacgag cattttttat
      781 tgccagattt actaactgtt tcttttcacc acgttgtggt ttaacaatct tacagcctac
      841 aattgtttca accgacttag catcaatatc ctgtggaata aagacttctt tgggtagaaa
      901 atgttttgta tcttgataaa attgccctat gtaagttaga aaatcttcct ctggttcatt
      961 ataataagga aacatattca cgtcacgctg aatcaatttt ccatttcgaa caaaaaagac
     1021 ctgtacacac atccaaccct tatcaacgaa ataaccaaaa acatctctat ccttcatatc
     1081 ttgatgaata acacgctgct tagtacgcaa caaactaatt gcttcgatca aatctcgata
     1141 ttcagccgca cgctcaaatt ccatagtcat agcagcgctc ttcatttttt cagttaatcc
     1201 gttaacaatt ttattatctt taccattaag aaactgtttt acatcttctc tcagactatc
     1261 ccaataagct ttattagttt gacaaactgt atgtgcattg cattgcccta aatgataata
     1321 aaagcatact ttatttgctg gattagtaca tttcttaaaa ggaaagagcc tatctagtaa
     1381 ccgcttaatc tcagttgctg ccccagaatc cggataaggt ccaaaataag tgccatctga
     1441 tttttttact tgacgtgtaa tcataagacg aggataacgt tcatttgtta ttttgatata
     1501 tgggtaagac ttatcatctt tcaatcgaat attatacttc ggcatatttt cttggattaa
     1561 attaatttcc aataatagag cttcggtatt agatgttgtc acaatgtatt caaagtcttc
     1621 aatctcagag accaaaagtt ctgtcttagt attatgactc ccatgaaaat aacttttcac
     1681 acgattctta agatttttag ccttacctac ataaataatt gtcccattct tatctttatg
     1741 aaggtaacaa ccaggactgt ctggtaacaa ttctagctta tgtttaatca gttcattcat
     1801 agaactatta taacaaaatg tactgtaatc tttacgaaat aaatactatt ccccaacatc
     1861 tattgtatct tccaatactt gaataaaacc ccaatactcc tagtctaagc aattatttta
     1921 tgttacactt taaacaagtg aattattgag gagtttttat gatagacgtt gagaatattc
     1981 ttgaaaaaat gaaacctaac caaaaaatca attacgattg ggtcatgcaa caaatggtta
     2041 agcagtggca agctagtgat atccgtccga aaattctgat gcatgtttgc tgtgctcctt
     2101 gtagtacata tacccttgaa tacttatcac aatgggcaga tgttaccatc tattttgcta
     2161 attcaaatat ccatcctaaa gatgaatatt atcgccgcga atatgttact caaaaatttg
     2221 ttcatgattt caacaaaaat actggatatt ctgtacaatt cttatcagca ccatatgaac
     2281 caaatgaatt tttcaaaatt gttcatgggt tagaagaaga acctgaaggt ggggatcgtt
     2341 gtaaagtttg ctatgatttc cgtcttgata agacagcgga aaaagccgtt gaactaggtt
     2401 ttgattactt tggcagtgcc ctaactatta gtcctcacaa aaactctcaa acaatcaata
     2461 ctattggtat tgatgtccaa aaaatttatg atactcagta tcttcctagc gaccttaaaa
     2521 aaaataaagg ttaccaacgt tccgttgaga tgtgtaaaga ttatgatatc taccgacaat
     2581 gttactgtgg gtgtatcttc ggtgctaaag atcaagggat aaatcttctc caaatcaaaa
     2641 aagatgcgaa agctttcgtg agtgataaag acggcaaaga agaatttcct aatatacgct
     2701 tcacatttaa tggcaagtca atgtaaatga taaaaaaaga cttagtaaag aactaagcct
     2761 ttttggtata aatcttgtca aactttaata atacaacttc ttaatctttt gcagacgttg
     2821 gtttagaaat agaccacaaa caaaaatacc tgtgattaaa ccaatccaat aggcaaacgg
     2881 acctaactgg ctattttttt ctaagataac cgctaatggc aaagcacata accaataaga
     2941 gcccgcaccg atcataaatg gttttgttgt atccttatag cctcgtaaaa tcccctgtac
     3001 aggagctgca taagcatctg caaactggaa aaagagacta taagttagga attgagctgt
     3061 aatagcgaca aagtgagggt cactattata cattgctgct acattctcac gaaatagaaa
     3121 taaaaatagt aaggttcctg atgtaatccc taccgctgtt aagcgtccta tcctactata
     3181 agtgtttgcg tcctgaaagc gctctgcccc tacttcaaac gatattgtaa tagctagagc
     3241 agtggaaata cttaaaggaa atgcatacat taatgatgaa aaattcatag cagcctgatg
     3301 tgctgcaata atgattgaag aaaattttgc catgaataag cctactactg caaaaattgc
     3361 aacttctgca aaaatttgta aaccaatcgg taatcccaat cgaatatctt caataatcaa
     3421 aggagctttt attctttcca gagtccatat atgatatgtt ttaatttgag gatgaagtga
     3481 catcacaata ataataacaa taaaaatagc ccaataagtt aaagaagttc caagacctgc
     3541 ccccgcacct cctagtctag gcataccaaa tttaccgtag ataagcatat aattaaaaaa
     3601 tgaattaaag ggtagaatta aaagcatcag atacatagat aaccttgtta accccaatgc
     3661 atcaaagaat gaacggcaaa tgctaaacaa caccagcggc atgattccaa tcaacatata
     3721 atttaaataa ccacgaccaa ctgctagaac ttcatcttct aaacccaaac tccccaagac
     3781 aggttgagca ataaattgca tgattaaaaa taatattaag gacagtatca aacctaaata
     3841 tagaaattga tgaaattctg tgcgaatttg ttctttattt cctctaccca aatgttgacc
     3901 aactactggt actaatgctg aaatcatacc tactaataaa gcgaaaaacg gagtccataa
     3961 attactagca gttgacacac ctgccaaatg tagctgacta tactgtccag tcataaccga
     4021 atcaataaaa gtagctgaaa aattagcaaa ttgataaatc aatactggga taaataattt
     4081 caaaaataaa acaaattttt ccttatttgt ctgagtttga tacatctttt ttctcctttt
     4141 caccttaatc aatttatttt agcttagcta ggatactctg tcaatgctat ttaaaaaggt
     4201 tgagaaataa tcctcaaccc cattttagct aagctcagta ttgatttgcc atgtcatacc
     4261 atatttgtca gtcacatttc cataagctgg actccacgga actgcttgta gaggcatttt
     4321 aatgttcatt gcatctcgag aaagcttatt gaataactct tgcgcttctt ctacagattc
     4381 cacaattaaa gctgctgtca tattttgtcc aatttgaaaa tcaaaaatag gattattgtc
     4441 agaaagttgc attcgaatac catcaatatt aaattgagcg ttcataatta aatgttcaag
     4501 ttctggcgga caatctggcc aaccctgtgc aaaagttgtg acgtgttctg cttttgtatt
     4561 aaaagcctct tcataaaatg taatagcttc ggccccacga ccattaatat tcatataaat
     4621 ttcaattgct ttcattacca tacctcactt catattatcc aaccaacgct gcatcagagt
     4681 ttcattttcc ccttttaggg taaaataaga accacgtatt atacctgcga gttttgactt
     4741 ttgacgagca aacttaaact tgtccgctaa ttcaatagga atatccattc catctgataa
     4801 ttttaaacct acagctctct ttttaggatt atctaattca tataaaatat taatggctaa
     4861 accatcctca tagaaaacat gtgtccataa aatacgattc tctaaaaaaa gctgtgaaat
     4921 ctctaaaggc tttgcagcaa aaacaaggtt acgttcattt tttagaaggt gattaatatc
     4981 tgccatcaat tctggatagt ctgatactgg aaatgtttcg taggctactt tgaatttatt
     5041 ccagaaatag cgagcttcat ttgcacgcaa accagccaag gcttctgcca ctggagaagt
     5101 ctccaaacct ttagttgaaa cggttttaaa gtcgataatc atgttctttc ctttttgatt
     5161 aaaatcttcg ataatttagt atactctttt ttgaagaaaa agtacattct aatcacttat
     5221 aaaaagtagt acatttagaa cactaccacc tcagtaattt cttgaccttt ctctacttaa
     5281 tacttataat taatctagac ttaatgtcaa agcatcaatc aaggtgtaaa cctgattcac
     5341 ctaaaaagct gatggagcta aacatgacca aaaagatatt cttatttatc tgcgctactc
     5401 ttttagtagg aatagcctta tatacaccaa caacttctat ttttaatcat aacaacgttt
     5461 atgctgccac caagaaagca aaaattaaat ttaataaaac tcaaaaaaaa attgtcaaaa
     5521 aagcaagaga atatgctaag tctggccata tgtctaaaga tagtatcatt gaaaaattaa
     5581 aaaaggattc taagaaatat cgtcaagaag acatcaactt tgtgattaat aacctcaagg
     5641 ttgattacaa aaaaaatgcc ttgatttctg ccaaaattta ttcaaagacc atgaatttat
     5701 ctaaacaatc tatctttgaa cagctctatt cggagtctcc agataaggca acccactctg
     5761 ataaatttac aaaagaagaa tcccaatatg ctatagacca tctcaaagta gatttcaaag
     5821 aaaatgctct tgagactgct aaaagttatc aaagtagttc ttctttatcc aaagaagaaa
     5881 tctataaaca attgacatct acgctgggcg ataaattcac aaatgatgaa gcccaatatg
     5941 ctgtagatca tttgaaatga tgaca
//
LOCUS       DATROQ010000105         5746 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.106, whole genome shotgun sequence.
ACCESSION   DATROQ010000105 DATROQ010000000
VERSION     DATROQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.106"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            329..1024
                     /locus_tag="VBC94_001803"
     CDS             329..1024
                     /locus_tag="VBC94_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO6980051.1"
                     /translation="MSKKSHRQYQIYEGLRCAVALCFISGYINAFTYVTQGKRFAGVQ
                     TGNLLSFAIHLSNKHYSQALAFLLPIMVFMLGQSFTYFMNRWANKHQLHWYLLSSFAL
                     TQVAIVTIILTPFLPSSFTVAGLAFFASIQVDTFKSLRGAPYANMMMTGNIKNAAYLL
                     TKGLYEKNSDIFLIARNTIIIIGGFIFGVVCSTYFSSKLGEWSLSLILIPLLYVNLLL
                     GHEFYNLQVERHS"
     gene            complement(1182..2057)
                     /gene="tehB"
                     /locus_tag="VBC94_001804"
     CDS             complement(1182..2057)
                     /gene="tehB"
                     /locus_tag="VBC94_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEO6980052.1"
                     /translation="MVNTKTLLPYKTMPVWTAQSIPKAFLEKHNTKEGTWAKLTILSG
                     SLVFYQLSPDGEEISRHIFDASSDIPFVDPQVWHKVSPNSPDLSCYLTFYCQKEDYFH
                     KKYGLTRTHSEVIASAPLLSEKSNILDLGCGQGRNSLYLSLLGHQVTSVDSNGQSLVA
                     LENMALEEELPYNIKRYDINTAAIEGHYDFILSTVVFMFLNPDCISDIILQMQSHTQI
                     GGYNLIVSAMDTAENPCPLPFPFTFKEGQLKSYYNDWEIIKYNENLGELHRVDENGNR
                     LKLQFATLLARKLRS"
     gene            2200..4197
                     /gene="metG"
                     /locus_tag="VBC94_001805"
     CDS             2200..4197
                     /gene="metG"
                     /locus_tag="VBC94_001805"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEO6980053.1"
                     /translation="MTEKKSFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGFD
                     VQYLTGLDEHGQKIQQKAEEAGITPQEYVDGMAESVKTLWELLDISYDKFIRTTDTYH
                     EEAVAKIFEQLLAQGDIYLDEYTGWYSVSDEEFFTESQLAEVYRDENGNMIGGVAPSG
                     HEVEKVSEESYFFRMSKYADRLKAYYAEHPEFIQPDGRMNEMLKNFIEPGLEDLAVSR
                     TTYTWGVQVPSNPKHVIYVWIDALMNYISALGYGWSDDLSQYHKFWPADIHMIGKDIL
                     RFHSIYWPIMLMALDLPLPKRLVAHGWFVMQDGKMSKSKGNVVYPEMLVERFGLDPLR
                     YYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTIAMVNKYFDGEVPRFAVAT
                     DFDADLASVATDSIENYHKQMEAVDFPRALEAVWNLISRTNKYIDETAPWVLAKDETD
                     RDKLAAVMSHLVASLRVVAHLIQPFMMETSDAIMEQLGLGATFDLEKLTFADLPEGVR
                     VVAKGSPIFPRLDMEDEITYIKEQMNAGKAPVEKEWVPEEVELTSSKGQIKFEDFDAV
                     EIRVAEVIEVEKVEGSDKLLRFRLDAGDEGHRQILSGIAKFYPNEQELVGKKLQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTVLTVDSAVANGSIIG"
     gene            4393..5730
                     /gene="brnQ"
                     /locus_tag="VBC94_001806"
     CDS             4393..5730
                     /gene="brnQ"
                     /locus_tag="VBC94_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO6980054.1"
                     /translation="MVKKGFLTGLLLFGIFFGAGNLIFPPALGVASGQDFWPAILGFC
                     LSGVGLAIITLLLGTLTNGGYKTEMSEKFSPWFSLTFLVILYLTIGPLFAIPRTATVS
                     FEIGVAPIVGHSPIALLCFTACFFAAAYYLAIRPNGILDSVGKILTPVFAFLILSLVV
                     VGAIAYGNLESAKASADYAGKAFGSGVLAGYNTLDALAAVAFCLVATETLKKFGFKTK
                     KEYLSTIWIVGIVTSLAFSILYIGLVFLGNKFPVPADILADPNVNKGAYVLSQASYKL
                     FGNFGRYFLSIMVTLTCFTTTVGLIVSVSEFFDKNFRFGNYKLFATVFTLIGFLIANL
                     GLNAVITFSVPVLTLLYPIVIVIVLIILINKWLPLSKKGMSLTIGLVTLVSFVEVLAG
                     QWQEKTLTQLVGFLPFHTISMGWLVPMLIGIVFSLVLSDKQKGQAFDLEKFEG"
BASE COUNT         1745 a          900 c         1124 g         1977 t
ORIGIN      
        1 actgacctcc taataggttg tatttattgt aacaaatgtt aagaatggtg caagtttttt
       61 ggtagtaaaa ttaaaagtaa ctaattaaac ttcaaattaa gggtaaatga cttatgagat
      121 tttctttatc ctttcataaa gtgagattgc ttcaatgctg aggctatgat atactaaaac
      181 tacatttatc attgcttaat aatttattta gaactgttag gctagctagt gctaatatta
      241 atactatttt aaaccaatct attagaaaaa aagtgctgag caagattgcc cagtcttatt
      301 tttgtttgcc ataaagttag aggaaaatat gtctaaaaag tctcaccgtc aatatcaaat
      361 atatgaaggg ctcaggtgtg cggttgcact ctgttttatt tcaggttata tcaatgcttt
      421 tacttatgta acacaaggaa aacgttttgc aggtgtacaa acagggaatt tgttatcttt
      481 tgctattcat cttagtaata agcattatag tcaagcgctt gcttttttgc tacctataat
      541 ggttttcatg ttaggtcaat cgtttactta tttcatgaat cgttgggcga ataaacatca
      601 actgcactgg tacctattat ctagttttgc gttgacacag gttgctatag taacaattat
      661 attgactcct ttcttaccat cgtcgtttac tgtggcagga ctagccttct ttgcttcaat
      721 acaagtagat acctttaaat cgctcagagg agcaccttat gctaatatga tgatgactgg
      781 taatatcaag aatgctgctt atttattaac aaagggactt tacgaaaaaa attctgatat
      841 tttcttaatt gctagaaata cgattattat tattggagga tttatctttg gtgttgtttg
      901 ctcgacttat ttttcaagta aactgggcga atggagctta tcattaatat taattcctct
      961 tttatatgtc aacttgctgt taggccatga attttataac ctacaagtag aaaggcactc
     1021 ctaatgtatg gcgttttatg ttgaagagcc atactaaagc tgattgttac aataatgatt
     1081 tgaaatatca tctctatata ttttatagga taattatgta tgggtttaga atatacttat
     1141 tacctaacca aagataaaag tgagtagatt tctactcact tttatgatct taattttcga
     1201 gctaataaag tcgcaaactg taattttaag cgattaccgt tttcatccac cctgtgcaat
     1261 tcacctaaat tttcattata tttgatgatt tcccagtcat tgtaataaga tttcaattgt
     1321 ccttctttga aggtaaaagg gaatggaagc gggcaaggat tttcagcggt atccatagca
     1381 gaaacgataa gattgtatcc tccaatctga gtatgtgatt gcatttgtaa aataatatca
     1441 gaaatgcagt cgggatttaa aaacataaat accacagttg ataaaataaa atcatagtgc
     1501 ccttcaatag cagcagtatt aatatcatac ctttttatat tgtaaggaag ctcttcttct
     1561 aatgccatat tttctaaagc tacaaggctc tgtccgtttg aatcgacaga agtcacttga
     1621 tgtcccagca gcgataaata aagtgagttt cgcccttgac cacacccaag gtctaatata
     1681 ttactcttct cagataagag aggtgcactg gcgataacct cagaatgtgt gcgcgtgaga
     1741 ccatattttt tatggaagta atcttctttt tggcagtaaa aagttagata gcaacttaag
     1801 tctggactat tcggcgaaac tttatgccag acttgtggat caacaaaagg aatatcacta
     1861 ctagcatcaa aaatatgccg cgagatttcc tctccatcag gagataactg gtaaaatact
     1921 aaagaaccac ttagaatggt tagttttgcc caggtgcctt ccttagtatt atgcttttct
     1981 aaaaatgcct ttggaataga ctgtgcagtc caaaccggca tagttttgta ggggagaagt
     2041 gtttttgtat taaccatata ttaactctct atttttaata cctttattat agcaaaagta
     2101 gatatattat tcagagatat atccagttga aaaagatttt atgttataat gtatcatgat
     2161 aaaaaacacc tctcattagg gtggcttata ggagaaatca tgacagaaaa aaaatcattt
     2221 tacattacta ccccaattta ttacccatca ggtaaattgc acatcggttc tgcctataca
     2281 acgatagcat gtgatgtttt ggcacgttac aaacgcatga tgggctttga tgttcagtat
     2341 ttgactggtc ttgatgagca tggtcagaaa attcaacaaa aggctgaaga agcaggcatt
     2401 acaccacagg aatatgttga tggcatggct gaaagtgtca aaacattatg ggaactttta
     2461 gatatttcct atgacaaatt catccgtaca acagatactt accatgaaga agcggttgct
     2521 aaaatttttg aacaattgtt ggcacaaggg gacatctatc ttgatgagta taccggatgg
     2581 tattctgttt cagatgaaga attttttact gaaagccaat tagcagaagt ttatcgtgac
     2641 gaaaacggta atatgattgg tggtgttgcg ccatctggtc atgaagttga aaaagtttct
     2701 gaagaatctt atttcttccg catgagcaaa tatgcagatc gtttaaaagc gtactacgct
     2761 gagcatccag aatttattca gccagatggt cgtatgaatg aaatgcttaa aaactttata
     2821 gaaccaggtt tagaagattt agcagtgtct cgtacaactt acacatgggg agttcaggta
     2881 ccatcaaatc caaaacatgt tatttatgtc tggattgatg ctttgatgaa ctatatttct
     2941 gctcttggtt atggttggtc tgacgatctc agtcaatatc ataaattttg gccagcagac
     3001 attcatatga ttggtaaaga tattcttcgt ttccactcta tttattggcc tatcatgttg
     3061 atggcgctgg atttgccatt accaaaacgt ctagttgctc atggttggtt tgtgatgcaa
     3121 gatggtaaaa tgtctaaatc aaaagggaat gtcgtttatc cagaaatgtt agtagagcgt
     3181 tttggtcttg atccactccg ttattatctc atgcgttcat tgcctgttgg ttcagacgga
     3241 acttttacgc cagaagatta tgttggacgt attaactatg aacttgctaa tgatttagga
     3301 aaccttctca accgcactat tgctatggtt aacaagtatt tcgatggaga agtaccaagg
     3361 tttgctgttg caactgactt tgatgccgat ttagcttctg ttgcgacaga tagtattgaa
     3421 aactatcata aacaaatgga agcggtggat ttcccacgtg cccttgaagc tgtatggaat
     3481 cttatttcac gaactaacaa atatattgat gaaacagcac cgtgggtact tgcaaaagac
     3541 gaaacagatc gtgataagtt agcagctgta atgagtcatt tagttgctag tctacgtgtt
     3601 gttgctcacc tcattcaacc gttcatgatg gaaacttccg atgccattat ggaacaactt
     3661 ggtttagggg caacattcga tcttgaaaaa ttgacttttg ctgatctacc agaaggtgtt
     3721 cgagttgttg caaaaggttc accaattttc ccaaggttgg atatggaaga cgaaattact
     3781 tatatcaaag aacaaatgaa tgctggaaaa gcaccagtgg aaaaggagtg ggttcctgaa
     3841 gaagttgaat tgacatcaag caaaggtcaa atcaagtttg aggattttga cgctgttgaa
     3901 atccgcgttg cagaagtaat tgaagttgag aaagtggaag gttctgataa acttcttcgt
     3961 ttccgtctag atgctggtga tgagggccat cgtcaaatct tatcagggat tgcaaaattt
     4021 tacccaaatg aacaagagtt agtagggaaa aaactccaaa ttgttgctaa cctcaaacca
     4081 cgtaaaatga tgaaaaaata tgtcagtcaa ggcatgattc tctcagcaga acatgatggt
     4141 aaattgacag tgttgacggt cgattcagca gtggcaaatg gatcaattat tggttaatcg
     4201 aaaagagcat tatgctcttt ttttgttttc aagaaaaaag tgactgaata gttggaaaat
     4261 tatgaaaatg acctagaatt tatttttatt tttgatataa taaaagacat cgattctaag
     4321 catttagata gaatttgata aggagaattt atggaaaggg tgtaaataga ttatttacac
     4381 taatggttgc ttatggtaaa aaaaggcttt ttaacgggat tactcttatt tggtatcttc
     4441 tttggtgcag ggaacctcat ttttccacca gcattaggtg tagcatctgg acaagatttc
     4501 tggccagcga ttttaggatt ttgtttgtca ggagttggat tagctattat taccctccta
     4561 ctaggcacac taactaatgg tggttataag acagagatgt cggaaaaatt tagtccgtgg
     4621 ttttctttaa catttttagt tatactctac ctaactattg gtccactttt tgctattcct
     4681 agaacagcaa cggtttcttt tgaaattggg gttgctccaa ttgtagggca tagtccgatt
     4741 gctcttttgt gttttacagc ctgttttttt gctgcagctt attatctcgc tattagacca
     4801 aatggtatat tagatagtgt tggaaaaatc ttaacgcctg tttttgcctt tttaattctc
     4861 tctttggttg ttgttggagc tattgcttat ggaaacttgg agagtgctaa ggcgagtgct
     4921 gactatgcag gaaaagcgtt tggaagtggt gtcttagcag gctataatac acttgatgca
     4981 cttgcagcag ttgctttttg tctagtagca acagaaacgc ttaaaaagtt tggttttaaa
     5041 actaaaaaag aatatttatc aaccatttgg attgttggaa ttgttactag tttagcattt
     5101 agtattttat atattggctt agttttttta gggaataagt ttcctgtccc agctgatatt
     5161 ttagctgacc caaatgttaa taaaggagct tatgtattat cacaagcatc ttataaatta
     5221 tttggaaatt ttggtcgtta tttcttgagt attatggtaa cattgacatg ctttacaaca
     5281 acagttgggc ttattgtgtc agtatctgaa ttttttgata agaactttcg ttttgggaat
     5341 tataaattat ttgctactgt ttttactttg attggctttt taattgctaa tcttggttta
     5401 aatgctgtaa ttactttttc agttcctgtg ctaactttat tgtatccgat tgttattgtt
     5461 attgtcttaa ttattttgat taataagtgg ttgccgcttt caaaaaaggg aatgtctttg
     5521 acaattggtc ttgtcacttt agtgtcgttt gttgaggttc tagcgggaca atggcaagaa
     5581 aaaacattaa ctcagttagt tggattcctc ccctttcata ctatttcaat ggggtggcta
     5641 gttccaatgt tgattggtat agtattttct ttggtgctgt cagataaaca aaaaggccaa
     5701 gcatttgatt tagagaaatt tgaaggatag cactatttca atttta
//
LOCUS       DATROQ010000106         5581 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.107, whole genome shotgun sequence.
ACCESSION   DATROQ010000106 DATROQ010000000
VERSION     DATROQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.107"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(108..1304)
                     /locus_tag="VBC94_001807"
     CDS             complement(108..1304)
                     /locus_tag="VBC94_001807"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEO6980055.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILEQDPDAHVAAE
                     TAVYTGSVHVFGEISTTAYVDINRVVRNTIAEIGYDKAEYGFSAESVGVHPSLVEQSP
                     DIAQGVNEALEVRGSLEQNPLDLIGAGDQGLMFGFAVDETPELMPLPISLAHQLVKKL
                     TDLRKSGELTYLRPDAKSQVTVEYDDNDQPIRVDAVVISTQHDPNVTNDQLHKDVIEK
                     VINEVIPSHYLDDQTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKVEVQLAYAIGVAQPVSVRVDTFGTGVIA
                     EADLEAAVRQIFDLRPAGIINMLDLKRPIYRQTAAYGHMGRTDIDLPWERVDKVQALK
                     DFIASK"
     gene            1489..2424
                     /locus_tag="VBC94_001808"
     CDS             1489..2424
                     /locus_tag="VBC94_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEO6980056.1"
                     /translation="MKTYEKIYQILSKNNNFISGETMANQLNISRTAIWKGIKTLEEL
                     GLEIESVTNKGYRLVSGDILLPEQLEQEIGIKVSLNNNSASTQLDAKMGIESKLKTPH
                     LFLAPNQKKAKGRFDRPFFTSNQGGIYMSLLLQPNVPIEDIKPYTVMVASSAVKAISR
                     LTGITPEIKWVNDIYLDNKKIAGILTEAIASVESGLVTNVIIGLGINFYIKEFPRALT
                     KRAGSLFTEQPTITRNQLITEIWNLFFNIPLEDHLKVYREKSLVLDKTVSFMDGQTMY
                     SGKAIDITDKGYLVVELDDGQLKTLRSGEISLSSW"
     gene            complement(2405..2599)
                     /locus_tag="VBC94_001809"
     CDS             complement(2405..2599)
                     /locus_tag="VBC94_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEO6980057.1"
                     /translation="MTKRQFIFMALLCSFETYFFNQSVMDGSWIFAIFWGVLLLRDLQ
                     KVYAISKFTKELIKSTKKKD"
     gene            complement(2688..4352)
                     /gene="dnaX"
                     /locus_tag="VBC94_001810"
     CDS             complement(2688..4352)
                     /gene="dnaX"
                     /locus_tag="VBC94_001810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEO6980058.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVSSKKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVNGEPCNHCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKLLAIRDHLAQILDKEAISYDLDALTLVARRAEGGMRDALS
                     ILDQALSLAKDNHISLDVAEEITGSISLSAIDDYVSNILAHDTTEALAKLEVIFDSGK
                     SMSRFATDLLMYLRDLLVVQAGGENSHSSDTFMANLDVKQDILFEMIDKVTSVLPEIK
                     NGSHPKVYAEMMTIQLSEMVEKNSSNIPADVTAELDSLRRELKSLKNEMSQLSRADQS
                     SSTQKVKVNNKTFTFKVDRTKILTIMEETVVDSQRSREYLEALKSAWNEILDNITAQD
                     RALLMGSEPVLANSENAILAFDAAFNAEQAMKRTDLNDIFGNIMSKAAGFSPNILAVP
                     RNDFNQIRSDFAKKMKAQKTETEPEVNHQIPEDFSYLAERIAIVED"
     gene            complement(4352..4849)
                     /locus_tag="VBC94_001811"
     CDS             complement(4352..4849)
                     /locus_tag="VBC94_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEO6980059.1"
                     /translation="MNKSKKIENYQLLLLQAQALFSDETNALANLSNASAMLNAMLPN
                     SVFTGFYLFDGEELILGPFQGGVSCVHITLGKGVCGESAQTAKTLIVDDVTKHANYIS
                     CDSKAMSEIVVPMFKNGKLLGVLDLDSSLVADYDEIDQEYLEKFVGILVEHTIWNLDM
                     FGVEK"
     gene            complement(4936..5565)
                     /gene="udk"
                     /locus_tag="VBC94_001812"
     CDS             complement(4936..5565)
                     /gene="udk"
                     /locus_tag="VBC94_001812"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEO6980060.1"
                     /translation="MRKKPIIIGVTGGSGGGKTSVSRAILSNFPDQKIIMIEHDSYYK
                     DQSHLTFEERVKTNYDHPLAFDTNLMIEQLNELIEGRPVDIPVYDYTKHTRSDRTIRQ
                     EPQDVIIVEGILVLEDQRLRDLMDIKLFVDTDDDIRIIRRIKRDMEERDRSLDSIIEQ
                     YTEVVKPMYHQFIEPTKRYADIVIPEGVSNIVAIDLINTKVASILNEAK"
BASE COUNT         1813 a         1080 c          892 g         1796 t
ORIGIN      
        1 gaaatatgca gtatttccta tacttaacca gtttattata agattttata aatgttctat
       61 aaattatcat gagcaaaaaa agcgtaaact ttcgtttacg ctttttatta tttgctagca
      121 ataaaatcct ttaaggcctg gactttatca actcgctccc atggcaaatc gatatcagta
      181 cgtcccatat gcccataggc tgctgtttgt cgataaatag gtcgtttgag gtccaacata
      241 ttaataatgc ctgctggtcg caaatcaaat atttggcgta cagctgcctc taggtctgct
      301 tctgctataa cacctgttcc aaatgtatct acacgcacag agacaggctg agcaacacca
      361 attgcataag caagttgtac ttctactttt ttagctaaat cagctgcaac aatatttttt
      421 gcaatataac gagctgcata agatgctgaa cgatctactt tagtagcatc tttacctgaa
      481 aatgcaccac caccatgacg agaataacca ccataagtat ctacaataat cttacgacct
      541 gttagtcctg aatctccttg agggcctcca ataacaaagc gccctgtagg attgataaag
      601 aattttgttt ggtcatctaa ataatggctt ggaataactt cattgataac tttctcaatc
      661 acgtccttat ggagttggtc attagttaca ttaggatcat gctgtgtcga aataactact
      721 gcatccacac gaattggttg atcattatca tcatattcaa ccgttacttg agattttgca
      781 tctggtcgaa gataggtgag ttccccagac ttacgtagat ctgtcaattt cttaactaac
      841 tgatgagcta aagaaattgg caatggcatt aactcaggtg tttcatcaac agcaaaccca
      901 aacatcaaac cttggtcacc tgcaccaatt aaatctaaag gattttgttc aagagaacca
      961 cgtacctcaa gagcttcatt gactccctgt gcaatgtctg gcgactgttc aacgagtgac
     1021 ggatgcactc cgacagattc agctgagaag ccatattcag ctttatcata gccaatttca
     1081 gcgattgtgt tacggacaac tcggttaata tcaacatagg ctgtcgttga tatctcgcca
     1141 aatacatgaa ccgaacctgt ataaacagct gtttcggctg ctacgtgggc atctggatct
     1201 tgctctaaaa ttgcatctaa aatcgcatcg gaaatttggt cagcaatctt atcgggatgt
     1261 ccctcagata ctgactcaga cgtgaaaagt ttacgttctg acataaaaat gtccccccta
     1321 aaatgtaaaa tgtgtcgagt tacaaagaac tagcctctag gctacctttt cgggactcat
     1381 atagctattg taccactatt tcatcttttg tgaaataagt taccaataaa taaaaaatta
     1441 tctctatttt ctttagtctt ttttctcgat ttatactata ctagatttat gaaaacctac
     1501 gaaaaaattt atcaaatact atcaaaaaat aacaacttta ttagcggaga aactatggca
     1561 aatcaactta atatttcccg cacagctatt tggaaaggta taaaaacgtt agaagaatta
     1621 ggcctagaga ttgagtctgt cactaacaag ggctaccgat tggtatcagg tgacatatta
     1681 ttaccagaac aactcgaaca agaaattggt attaaggtta gtcttaataa taatagtgct
     1741 tcaacccaac tagatgctaa aatggggata gaaagcaaat taaagactcc acaccttttt
     1801 ttagctccta atcaaaaaaa agctaaagga cgttttgata gacctttctt tacatcaaat
     1861 caaggcggca tctatatgtc tcttttatta caacctaatg taccgattga agatataaaa
     1921 ccttatactg tcatggtagc ctctagtgct gtaaaagcta tctcacggtt aacaggaatt
     1981 actcctgaaa ttaagtgggt caatgatatt tatttagata ataaaaaaat agctggtatt
     2041 ttaaccgagg ctatcgcttc tgttgagagt ggattggtaa cgaatgttat tataggatta
     2101 ggtatcaatt tctatatcaa agagttccct agggcgttaa ctaagcgcgc aggtagtctc
     2161 tttacagagc aaccgaccat aactagaaat caattgatta cagaaatctg gaatctcttt
     2221 ttcaatattc cactagagga tcacttaaag gtatatagag aaaaatccct agtcttagat
     2281 aaaaccgtta gctttatgga tggtcaaaca atgtattctg ggaaagctat tgatattact
     2341 gataaaggat atttagtggt tgagttagat gatggtcagt tgaaaacact tcgcagtggc
     2401 gaaatcagtc tttcttcttg gtagatttga taagctcttt tgtgaatttt gaaatcgcat
     2461 acaccttttg aagatctcga agtaataaaa ctccccaaaa aatagcaaaa atccaggagc
     2521 catccataac ggattgattg aaaaaataag tttcaaaaga gcataaaagc gccataaaaa
     2581 taaattgtcg ttttgtcata tcaccattat aacagaatct actaaataat taaaaaactt
     2641 atcaaaaaaa tagttgcctg ttacgggcaa ctatttttag ttacgtttta atcttcaacg
     2701 attgctattc gttctgctag ataagaaaag tcttctggaa tctgatgatt aacttcaggt
     2761 tctgtttccg tcttttgagc tttcattttc tttgcaaaat cgctccgaat ttgattaaaa
     2821 tcatttctag gtacagctaa aatattaggt gaaaaacctg ctgctttact cataatatta
     2881 ccaaatatat cattgagatc ggtacgtttc atagcttgtt cagcattaaa ggccgcatca
     2941 aatgccaaaa tagcattctc gctattagct aagacaggtt cagatcccat taataaggct
     3001 ctatcttgag ccgtaatatt atctaatatc tcgttccaag cactctttaa agcttctaaa
     3061 tattcacgtg aacgttggct atctacaacc gtttcttcca taatggtcaa aatctttgta
     3121 cggtcaactt tgaatgtgaa tgtcttatta ttaaccttta ctttttgagt tgatgaactt
     3181 tgatctgctc gagagagttg gctcatttcg ttttttaacg attttaactc tcgacgaaga
     3241 ctatctaatt ctgctgtcac atctgcgggt atgtttgacg aatttttttc taccatttca
     3301 gacaattgaa tggtcatcat ctctgcataa actttaggat ggctaccatt ttttatctca
     3361 ggaagaacag atgtcacttt atcaatcatt tcaaataaaa tatcttgttt aacatcaaga
     3421 ttggccataa aagtatcact tgaatgacta ttttcaccac ctgcttgaac aacaagtaaa
     3481 tctcttaagt acatcaataa atctgttgca aaacgactca tacttttgcc actatcaaag
     3541 attacttcaa gtttagcgag tgcttcagtt gtatcgtgag ccaatatatt cgatacataa
     3601 tcatctatag cagataagga aatgctccct gtaatttctt ctgctacatc taaagaaatg
     3661 tggttatctt tcgctaaact caaggcttga tcgagaattg ataaagcatc acgcatacca
     3721 ccttcggctc gtctagcgac tagtgtcaga gcatccaaat catatgaaat agcttcttta
     3781 tctaaaatct gtgctaaatg atctcgaata gctaataatt taatcgcttt aaattcaaag
     3841 cgctggacac gagataagat tgttgcaggg attttatgga gttctgttgt tgctaatata
     3901 aaaacaacat tttctgttgg ctcttcaagt gttttcaata gtgcattgaa ggcacctgta
     3961 gacaacatat gaacttcatc aataatataa actttatagg ttgccctact tggagcataa
     4021 gttgatttgt ccctgatatc acgaatttca tcgacaccat tatttgacgc agcatcaatt
     4081 tcaatgacat cctctaaact accatttgta atatctcgac aaatatcaca atgattacaa
     4141 ggttcgccat taacttgatt tgggcaattc atcgcttttg caaatatttt agcagcactt
     4201 gtttttccag ttcccctagg tcctgaaaaa aggtaagcat ggctaatttt tttgctggac
     4261 acagcttgct ttaaagtagt tgaaataaca ctttgtccaa ccatttcatc aaatgtttgg
     4321 ctacgatatt tccgataaag ggcctgatac attacttttc aactccaaac atatccaaat
     4381 tccaaatcgt atgttctact agaataccta caaatttttc taagtattct tgatcaatct
     4441 catcataatc tgctactaaa gaagaatcta aatctagaac tcctagaagt ttgccatttt
     4501 taaacatggg tactacgatt tcactcatag cttttgaatc acaggagata tagttagcat
     4561 gctttgtaac atcatcaaca atcagcgtct tagcagtttg tgcagattca ccacaaacac
     4621 cttttcctaa agtaatatgc acacatgata caccaccctg aaaagggcca agaattaact
     4681 cctctccatc aaataaataa aagcctgtaa atacagaatt tggaagcata gcatttagca
     4741 tagctgaagc atttgataag ttggcaagag catttgtttc atctgagaat agagcttgcg
     4801 cttgtagtaa taataattga taattttcga ttttcttaga cttgttcata gcacttatta
     4861 tagcataatt tgaaagttac taaaactaaa taaccatcct atataaaaca aaaaaataaa
     4921 cacaaaccat tgtgtttatt ttgcttcatt taagatgcta gccactttgg tattgattaa
     4981 atcaatagcg acaatattac ttactccttc tggaattaca atatccgcat aacgctttgt
     5041 tggttcgata aattgatgat acataggttt tacaacttct gtatactgtt caataatact
     5101 atctaaactt ctatcgcgct cttccatatc acgcttaata cgacgaataa tacgaatatc
     5161 atcatctgta tcaacaaata atttgatatc cattaaatca cgcaaacgct gatcttctaa
     5221 taccaaaatt ccctcaacaa taatgacatc ctgaggttct tggcggatag ttctatcact
     5281 acgggtatgt tttgtgtaat cataaaccgg aatatcgacc ggacgcccct caatcaattc
     5341 gttcaattgc tcaatcatca aattggtatc aaaagcaagg ggatgatcat aattggtttt
     5401 tacacgttct tcaaaagtca aatgactttg atctttatag taagaatcat gttcaatcat
     5461 gataattttt tgatctggga aatttgataa aatcgcccga gaaacactgg ttttaccacc
     5521 accagatcca cctgtaacac caataataat aggtttttta cgcatttgtc actccaaatt
     5581 a
//
LOCUS       DATROQ010000107         5357 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.108, whole genome shotgun sequence.
ACCESSION   DATROQ010000107 DATROQ010000000
VERSION     DATROQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.108"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..1016)
                     /locus_tag="VBC94_001813"
     CDS             complement(<1..1016)
                     /locus_tag="VBC94_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEO6980061.1"
                     /translation="MFLLLCILSMWLMIFGVAFYYFGSLHQSLTSRIIYQFVLTVLLT
                     TTAWFMGAYFLAFEGHFKTVFQFQEADGKQIVNCLFQLCFALYAVVMLIGSIIDRVQT
                     KRLLLAVVSWLFLVYTPLAYLIWNSEGVFAKMGVLDFSGGMVVHLSAGLSSYILAHVI
                     GKSEHQHNKVKNDSLFLGMILITFGWFGFNMGPVGEWNSQAIMILLNTIFAIIGGGLA
                     WTLAAKWNGEEEKTGSLLNGIIVGLVTSTAGVGYLLTWQLLAVTFFASLFTYFVTDYV
                     AKAFAIDDVVSSFGMNGIGGLLGSLGVGLFKLSHMPVQLLALATTILLSIIMTYIISK
                     AIFR"
     gene            1120..2247
                     /locus_tag="VBC94_001814"
     CDS             1120..2247
                     /locus_tag="VBC94_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEO6980062.1"
                     /translation="MKHYQTIIIGAGAAGIGFGSAMQRLGLTNFLIIEKGHIGESFLR
                     WPRTTQFITPSFTTNGFGFPDLNAVIPDTSPAFSFEKEHLSGVEYARYLQLVAAHYNL
                     PIQNETSVLSIDKRDSLFVIKTSKGDFSADYLIMATGEFQNPNTIDIKGADLGMHYGQ
                     VDNFHIKSDNPFIIIGGNESACDALTHLVYLGNQVELYTDTFGRKESNPDPSISLSPL
                     TKERLKHIQDHKKEYYSISEGKKAIEIKQIGKQYQVTFDDGSTAESFHKPILSTGFLN
                     TCHLIDGIALFEYDKNQLPIVTEDDESTIVNNCFLIGPSLRQRDTIFCYIYKFRQRFV
                     PVINHIAQREGIILDEDTLEFFKENQMYLDNLACCDVNCDC"
     gene            complement(2284..3270)
                     /locus_tag="VBC94_001815"
     CDS             complement(2284..3270)
                     /locus_tag="VBC94_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEO6980063.1"
                     /translation="MSEKIRVLLYYKYVSIENAEEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWIHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENEAKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPQVANI"
     gene            3433..3739
                     /locus_tag="VBC94_001816"
                     /pseudo
     CDS             3433..3739
                     /locus_tag="VBC94_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3897..5270)
                     /locus_tag="VBC94_001817"
     CDS             complement(3897..5270)
                     /locus_tag="VBC94_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6980064.1"
                     /translation="MLKKLFAPPQKRERLSAEEIKHKYPRYRVQVLISIFVGYMGYYF
                     VRNTTSILSGILNMSATEIGIITCASYIAYGLSKFISGLISDESNSKIFLPVGLFLTG
                     LVNVLIGVIPSVITSIWLFTIMYLINGWLQGMGYPPGARTLVYWYDNKERIKYATIWN
                     LSHNFGGAIAPILTGVGLALAGNDSLNQARAAYWFPGVVACLLAVLVYFLQEDTPESI
                     GLPPIEEYHKEQYTNVVDSSDILEEPEVLGMGEIIKKYILPNTKLMWASLYSIFVYIL
                     RYGIVSWTPKFLATSVQDGGKGITATAGMGGFSLFEIGGIIGMLTAGYLSAKVFKNSK
                     PLTNVAFLVVAILLLAAYWFIPAGPQYMALDFIILLGLGASIYGPVMMVGLYAMELVP
                     KAAAGAASGLTGTFSYVGGATIATLAIGIIIDHFGWGVAFIIFGISGFAAIVCTLLSR
                     DKSLEYW"
BASE COUNT         1884 a         1094 c          786 g         1593 t
ORIGIN      
        1 tttctaaaaa tcgctttaga tatgatatag gtcattataa ttgacaaaag aattgtggta
       61 gctaatgcta acagttgaac tggcatatgt gataatttaa agagaccaac tcctaaactt
      121 cccaacaatc ccccaatacc attcattcca aaggaggaaa cgacatcatc aatggcaaaa
      181 gctttcgcaa cgtaatccgt tacaaagtag gtgaagaggg atgcaaagaa tgttacagct
      241 aataattgcc aagttaagag atacccaact cctgctgtac tcgtcacaag accaacaata
      301 atgccattca ataagcttcc tgttttctct tcctcaccat tccacttagc agccaatgtc
      361 cacgccaaac caccacctat aatagcaaat atagtattta aaagtatcat aatggcttgt
      421 gaattccact caccaactgg acccatatta aatccaaacc aaccaaatgt aataagtatc
      481 attcctaaaa agagactatc atttttaacc ttattgtgct gatgttcact cttgccaata
      541 acatgagcta aaatatacga agaaagccct gcagataaat gaacgaccat tccgcctgaa
      601 aaatcaagta ctcccatctt tgcaaaaaca ccctctgaat tccaaattaa ataagctaag
      661 ggcgtataca ccaagaaaag ccaagaaaca acagctaata aaagtcgctt cgtctgaact
      721 ctatcaataa ttgacccaat aagcattact actgcgtaaa gggcgaaaca aagttgaaat
      781 aaacaattta caatttgttt accatctgct tcttggaatt gaaagacagt tttaaaatga
      841 ccttcaaatg ctaaaaaata ggcacccatg aaccaagctg ttgtcgttag caaaacagta
      901 agtacaaact gataaataat ccttgaagtc aatgattgat gtagcgaccc aaagtaatag
      961 aatgcaaccc caaaaatcat gagccacatc gataaaatac ataatagtaa aaacacaaat
     1021 agtcctttct ttacaaacac tcttttattt gttaaactgt attgttgtta actggttaag
     1081 tattttatct tacacattta tttttgtcaa ggagaagaca tgaaacatta tcaaaccata
     1141 attatcggtg caggcgctgc aggaatcggt tttggttctg ccatgcaacg attaggactt
     1201 acaaattttc ttatcattga aaaagggcac attggagaat cttttttaag atggcccaga
     1261 acaacacaat ttatcacacc ttccttcact accaatggct ttggttttcc agatctaaac
     1321 gctgttattc cagatacttc tcctgctttc tcatttgaaa aagaacatct ttctggagtg
     1381 gaatatgcac gctacttaca attagttgct gctcattata atctccctat ccaaaacgaa
     1441 acatctgttc tatctattga taaacgagat agcctttttg ttattaaaac ttcaaaggga
     1501 gatttttctg ctgattatct tattatggca acaggagaat ttcaaaatcc aaataccatt
     1561 gatattaaag gtgcagatct aggaatgcac tatggtcaag ttgataattt ccatatcaaa
     1621 tctgacaatc catttatcat cattggtgga aacgaaagtg cttgtgatgc actaacacat
     1681 ctagtatatt tgggaaatca agtcgaatta tatacagata cgtttggtcg taaagagagc
     1741 aatcccgatc caagcatctc tttatccccc ctaactaaag aacgtttaaa acatatacaa
     1801 gaccacaaaa aagaatatta tagtatctct gaaggcaaga aagccattga gattaaacaa
     1861 attggaaaac aatatcaagt tacttttgat gacggtagta ctgcggagtc tttccataaa
     1921 cctattcttt ccacaggttt tctaaatacc tgtcatctca ttgacggaat cgctttattt
     1981 gaatacgata aaaatcagtt gccaatagtt actgaagatg acgaatctac tatcgttaat
     2041 aactgtttct taattggtcc aagcttacga caaagagata ctattttctg ctatatttat
     2101 aaattcagac agcgctttgt ccctgttatt aatcatatcg cacaacgaga agggattata
     2161 ttagacgaag atacccttga attctttaaa gaaaatcaaa tgtaccttga taacctagct
     2221 tgttgcgatg tcaattgtga ttgctaaaaa atcgctagct ttattgctag cgatttttta
     2281 atattaaata tttgctactt gaggtaagga ttctccaata gcttctaaac gttctgccca
     2341 ttcttgacgt gataaaccat tttcttgcac atagcgatta cgttcatgtg cacgacattc
     2401 aggcgagcac cctcgaacat acttcgcttc gttttcttct gaagcaaaaa tttgtttgtt
     2461 acaaaacgga tttgcacaat tgacataacg ttcacaaggt gttccatcaa aataatcttt
     2521 actaataaca gttggattaa catgattaat tggcaccgag atacgatcat caaaaacata
     2581 catcgcacca tcccaaagtt caccttgaac ttccggatct tttccatatg ttgcaatacc
     2641 accgtgtaat tgtccaacat ctttaaaacc ttctcgaacc atccaacccg agaatttctc
     2701 acaacgaaca ccaccggtac agtacacaac aacacgtttt tccataaact tatctttgtt
     2761 gtcacgtacc cattgtggta actcacggaa attgcgaata tcaggacgaa ttgctccacg
     2821 gaagtgacct aagtcatact cataatcatt acgcgtatct aaaacaacag tatcttcatc
     2881 aagcaaggct tctttaaatt gtttaggatt taaatactca cctgttgttt ccaaaggatt
     2941 aatatcgcta tcaaagttat tatcttctaa cccaaggtgc acaatctctt tcttataacg
     3001 aacaaacatt ttacggaaag cttgttgatt ttcctcgtca atcttgaacc aaagatcagc
     3061 aaaacgctca tcgctgtgga tccaatccat atacttctga gttgtttcat aatctccaga
     3121 aacagttcca ttaatcccct catccgcaat caaaatacga cctttaagcc caatagattt
     3181 acaaaattct agatgtttcg cagcgtactc ttccgcattt tcaattgaaa catatttata
     3241 atagagtaaa actctgattt tttctgacat tttttctcct catctgcaaa ataagttagt
     3301 atacttcatt atataacgac tccttacaac atagcaaaaa atacgcttag gacatattct
     3361 ttgctttaaa gacaaattat tcttcttaag aacacacata tgttaaaata gtaaaagact
     3421 aggaggactt ttatgactta tgaattatgt ctagaatacg gtacttatcc acttagacct
     3481 gtagatgctt gggctgatga aatcaatact gcacctgcgt ttattactga agataaaaaa
     3541 acttttagaa cttttagaag aagtcaatac cttattccat gagctctttt taacgataga
     3601 atgttccttc cactatattg gtcatgactt cccagaaaaa agagcaaaaa tcacacaaat
     3661 ttatcatgtg attatcgaac atttaagcat acactaccct gaatatgaca ttaaaattga
     3721 atcattatta atggggtagt tttattttaa ctaggtagaa actatctatt aaaaacaatc
     3781 gatatcacta attaattcaa caaatagatt atctaaaaca aaaaaggtct ctttcaagag
     3841 gccttttttc ataacttatc tcactaaata ttaaatgata cttttaccaa ataatatcac
     3901 caatactcta gactcttatc tcttgaaagc aaggtacaga caatagccgc aaatccagaa
     3961 ataccaaata ttataaatgc tactccccaa ccaaagtggt caataataat accaatagct
     4021 agagtagcga tcgttgctcc tccgacataa ctaaatgtgc cagtcaatcc tgaagctgct
     4081 ccagctgctg cttttggaac taactccata gcatacaggc caaccatcat aacaggaccg
     4141 tagattgatg cccccaaacc tagtaaaata atgaagtcta atgccatata ttgaggtcct
     4201 gctggaataa accaataggc agctagtaat aagattgcta caactaagaa ggctacattg
     4261 gttaaaggtt tagagttttt aaacactttg gcagatagat atcctgctgt tagcatccca
     4321 ataataccac ctatttcaaa aagtgaaaaa ccacccatac cagctgttgc agtaatacct
     4381 tttccaccat cttgcacact cgtcgctaaa aacttaggag tccatgagac aataccgtaa
     4441 cgtaaaatat atacaaatat tgaatacaag gatgcccaca tcagtttagt atttggtaga
     4501 atatattttt ttataatttc gcccataccc aaaacttcag gctcttctaa aatgtcactt
     4561 gaatccacaa cattagtata ttgctcttta tgatactctt ctataggcgg aagtccgata
     4621 gattctggag tatcttcttg taaaaagtaa acaagaacag ccaaaagaca agccaccacc
     4681 cctggaaacc aataagcagc acgcgcttga ttaagagaat cattaccagc aagagctaga
     4741 cctacaccag ttaaaatcgg cgctattgct ccaccaaaat tgtgagataa attccaaatg
     4801 gtagcatatt taattctttc tttattatca taccaataga ctaatgtacg cgctccagga
     4861 gggtatccca tcccttgtaa ccaaccatta ataagataca tgatagtaaa gagccagata
     4921 ctagtaatta cagatggaat aacaccgatt aacacattaa ctagtcctgt aagaaacaaa
     4981 ccaactggaa ggaaaatttt agaattcgat tcatctgaaa tcaaaccaga aataaattta
     5041 gagagaccat aagcaatgta gctagcacag gtaataatac caatctcagt agcagacata
     5101 ttaaggatac cagataaaat tgaagtcgta ttacgaacaa aatagtatcc catataacct
     5161 acaaaaatag aaataaggac ttgtacacga taacgaggat atttatgctt aatttcctca
     5221 gctgataaac gttctctttt ctgagggggt gcaaatagtt ttttcaacat agcaaattac
     5281 ctttccgatt tttagatgtg tttaattata caactatgaa ttcacactgt caattaatta
     5341 ataatttatt ttttatc
//
LOCUS       DATROQ010000108         5306 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.109, whole genome shotgun sequence.
ACCESSION   DATROQ010000108 DATROQ010000000
VERSION     DATROQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.109"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            14..487
                     /locus_tag="VBC94_001818"
     CDS             14..487
                     /locus_tag="VBC94_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6980065.1"
                     /translation="MFAVTQDILFIDAHSQEELFDLVSKALIKQHYVSPDYRQAVKER
                     EREFPTGLKIDLKDGTPIQYVAIPHTETQYCLVDRIFYVKNSQPITFKHMINPEEECR
                     VQDFFFIINSRNSNQSDILSNLITFFITKGNLDRLHELGDNKEKINHYLIEKGVF"
     gene            489..794
                     /locus_tag="VBC94_001819"
     CDS             489..794
                     /locus_tag="VBC94_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6980066.1"
                     /translation="MIKILAACGAGINSSHQIKDAIETQLGDRGYNVHCDAVMVKDIT
                     EEMVNKYDIFTPIAKTDLGFNVPIPVVEAGPILYRIPVMSEPVFTALEQVIKEHNLS"
     gene            834..1321
                     /locus_tag="VBC94_001820"
                     /pseudo
     CDS             834..1321
                     /locus_tag="VBC94_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
     gene            1562..4011
                     /locus_tag="VBC94_001821"
                     /pseudo
     CDS             1562..4011
                     /locus_tag="VBC94_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771303.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
     gene            4261..4685
                     /gene="lacA"
                     /locus_tag="VBC94_001822"
                     /pseudo
     CDS             4261..4685
                     /gene="lacA"
                     /locus_tag="VBC94_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
     gene            4706..5221
                     /gene="lacB"
                     /locus_tag="VBC94_001823"
     CDS             4706..5221
                     /gene="lacB"
                     /locus_tag="VBC94_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEO6980067.1"
                     /translation="MKIAVGCDHIVTYDKIAVVDYLKTKGYEVIDCGTYDNIRTHYPI
                     YGKKVGEAVASGKADLGVCICGTGVGINNAVNKVPGIRSALVRDLTSAIYAKEELNAN
                     VIGFGGKITGGLLMTDIIEAFIRAKYKPTKENKVLIEKIAEVETHNAHQEENDFFTEF
                     LDKWNRGEYHD"
BASE COUNT         1881 a          752 c         1048 g         1625 t
ORIGIN      
        1 actaaaaaga aaggtgtttg ctgtgactca agatatttta tttattgatg cccattctca
       61 ggaagaatta tttgacttag tatcaaaagc attgataaaa caacactatg ttagtcctga
      121 ctaccgacag gcagtaaaag aaagggagag agagtttcca acggggttaa aaattgattt
      181 aaaagatggg actcctattc aatatgttgc tattccacac actgaaacgc agtattgtct
      241 agtagataga atcttttatg tcaaaaatag tcagccaatt actttcaaac atatgattaa
      301 tccagaagag gagtgtaggg ttcaggattt tttcttcatc attaatagta ggaatagtaa
      361 ccaaagtgat attttatcta atttgattac cttctttatt acaaaaggaa acctagatcg
      421 gctacatgag cttggcgaca ataaggaaaa aattaatcat tatttaatag aaaaaggagt
      481 gttttaacat gatcaaaata ttagcagctt gtggtgcagg cataaattca agtcatcaaa
      541 ttaaagatgc tatcgagaca caattaggag atagaggtta caatgtacat tgtgatgctg
      601 ttatggttaa ggatatcact gaggaaatgg tcaacaaata tgatatcttt actcctattg
      661 ctaaaactga tttaggtttt aatgtaccaa ttccagttgt agaagcagga cctattcttt
      721 atcgcatacc agttatgagc gaaccggtct ttactgcttt agaacaagtt atcaaagaac
      781 acaacctatc ataacatatt taaaattgga attagtctta aaggagaagg aacatgcagg
      841 tatttttaaa tattgtcaat aaattctttg atccagttat tcatatgggt tcgggagttg
      901 tgatgctaat tgtcatgaca ggtttagcca tgatatttgg agtgaagttt tctaaagcac
      961 ttgaaggtgg tattaagtta gctattgctc ttacgggtat tggtgctatt attggtattt
     1021 taactggtgc tttttccgaa tcacttcaag cttttgttaa aaatacagga atcaatctaa
     1081 gcattattga cgttggttgg gctccattag caactattac atggggatca ccatatacgc
     1141 tttacttctt attaatcatg cttattgtca atattgttat gattgttatg aaaaaaaaac
     1201 ggatacctta gatgttgata tttttgatat ttggcattta tctatcacag gtcttttaat
     1261 tatgtggtat gctaaacaaa tgaaacgccg taatgcagaa tatgcagcta atcaaaaata
     1321 aatagaataa acaaaaacct aggaaatatc acattctcct aggtttttga tatattaaat
     1381 attttaaaaa taatatatat aattaatttt cgctcatttt aataataaaa aactagtaaa
     1441 attataaaaa tatattgaaa atagtgataa gttggtatac aatccttttt ggaaacatta
     1501 ctgaactccc atttttctag agttgatgta tgcaaccaat attttaagac ttggagtttc
     1561 tatgacatta acctttggga aaccttctat aacgatacgc cgtctaggag tgataactag
     1621 tacatatatg atgctaggtt ttatcttatt atgtggactt ttgcatccta gtacaattta
     1681 tagtgaggaa ataaaatctg atataaactc ttttatatca atagatgatg aaaaaactac
     1741 tgctcccata tttgactcta ctttagataa gcttactaat tcaacagagg agaggcagtt
     1801 agaattagat tctgatacat cggacggttt aatagtagat gatcaaaaag ataaagatat
     1861 ggtagtagtc ttaggtgatt ctgacagtca agaaaaattg tcacctcctc taaaatcgga
     1921 aatagcgaca gaagaaaatc gtaaacaggc aaatttagca gaactaaatg caactcaacc
     1981 taataataga actacctata ttatacccga aagcagtcat tccattgcag aacaacagag
     2041 attcctgata gaatcaaagg gttcttcggt tgcattactt aatagcgatg aatttagaaa
     2101 gacagcggga gaggatagag gttttgaaag ggataagttg aggtctttgg atatcattcc
     2161 taagggagat ttatcgacaa gtaatgtcat aggtaatacg gacattgcta gtcagatatc
     2221 gttgggcttt aaaagaatgc gatgcaggaa caccatctta ctaaaacatt ctctcaaaag
     2281 gatggaaagt tatcgtctgt tatagagggg atgcttgcta ttggcaaaga gaaagtagag
     2341 aaagaaataa aatatagtgg taatttatgg caaaaattaa aagctaaggc acactgcctt
     2401 gtttgctgtg ttgataattt gaattttgaa gatataaaat cttattttca atattattgt
     2461 catctaaacc atcagctcaa attacctaaa ggtgctatac tttctgctaa aacagaagta
     2521 tataggggag gagattttgg gagaaaaaat aaagataatg tgtttggtta ccgtatcccc
     2581 tcattattga aaacccaaaa aggaacttta cttgcgggag ctgatgaaag aattgagcaa
     2641 gcttgtgatt ggggaaacat aggaatggtt attcgccgta gtgaggatga tggtgtcact
     2701 tggggaaaaa gagaaactat tgtcaatctc cgtaataacc ctagagttcc gctagttact
     2761 agtggtgact atagtggctc acctattaat atggatatgg cattagttca agatactagc
     2821 tccaagacga aacgtatttt ttcaatatat gatatgtttc cagaaggaag aggcgttatt
     2881 agtattgcta acacacctga aaaagaatat acccaaatcg gaggacagtc ttatcttaat
     2941 ttatataata atggaaagaa atcgaaggtt tttactatcc gtgacaaagg tattgtatat
     3001 aattttaaag ggaaaaagac tgattatcat gttataacag aaactactaa aagtgaccat
     3061 tcaaatctag gggatattta taagggaaaa cagctacttg gaaatatata ttttacaaaa
     3121 cataaaacgt caccatttcg tttagcaaaa tcaagctatg tgtggatgtc atatagcgat
     3181 gatgatggta ggacatggtc atcacctaga gatataacag caagtcttcg tcagaaaggc
     3241 atgaaatttt tgggaatagg acctggaaaa ggtatagttt taaaatgggg gccacacgct
     3301 ggtcgtatta ttattcctgc ctattctacg aattggaaat ctcatctaag aggttcacaa
     3361 tcttcacgcc taatttattc agacgaccat ggaaaaacgt ggcatactgg aaaagcagtc
     3421 aatgataacc gtatactttc taatggtgaa aaaattcact ccttaacaat ggataataaa
     3481 aaagaacaaa atacagaatc cgtacccgtt caattgaaaa atggggacat taagttattt
     3541 atgaggaatc taactggtaa cctagaagta gccacaagta aagacggcgg ggagacttgg
     3601 caaaaccatg ttaaacgata taaggaaatt catgatgctt acgtccaact atcagctatt
     3661 cgctttgagc atgacaaaaa agagtatatt ttattagtga atgctaatgg gccagggaag
     3721 aagtgccaag atggatatgc acgtctagcg caagttaatc gaaatggtag ttttaagtgg
     3781 ttatatcacc atcacattca agatggttcg tttgcttaca actctgttca acaacttaat
     3841 aatgatcaat ttggtgtcct ttatgaacat agagaaaaac atcaaaatag ttttacttta
     3901 aattacaaag tttttaattg gagttttctt agtcaaaata cagagaagca aggcacttta
     3961 tgggagaaaa tggcagcaaa ttggcatgtt ttgtttaaat tttatttatg attatggctt
     4021 gcggaataac cgttcctaca aataaaaata atgagaagta tcaaacttcg tgatagctag
     4081 ctagtagtga attctagttg ccatagttat cacggagttt tttttgttta tttcaaacaa
     4141 aaacaaaaac aaaacattga caaaaacaaa aataagatat atgatgatat tacaaacaaa
     4201 aaacaaacaa tatcttccaa ctacatacaa ttgtattaaa agtgaaataa ggagaaagaa
     4261 atgacaatta ttattggggc ggatgcgcat ggtgttgaac ttaaggaagt gattagacaa
     4321 caccttacac gtttaggtaa ggaaattatt gatttaacgg atactagcaa agatttcgtt
     4381 gataatacct tagcaatcgt tgctaaagtg aatcaaaaag aagataactt aggaataatg
     4441 gttgatgctt acggtgctgg tccttttatg gtagccacta aggttaaggg gatgattgca
     4501 gcagaagttt cagatgaacg ctcagcttat atgactagag ctcataataa cgctaggatg
     4561 attactttag gttcagagat tgttggtccg gggttgctaa acatatagta gagggttttg
     4621 ttgatgggac ttacgatgca ggtcgtcatc aaattcgggt cgatatgttg aataaaatgt
     4681 gctaagcgta agagaggtaa agcagatgaa aattgcggtc ggttgtgatc atattgtaac
     4741 ttatgacaaa attgcagttg tggattattt aaaaacaaaa gggtatgagg ttattgattg
     4801 tggaacttat gataacatcc gtactcacta tcctatttat ggtaagaaag taggagaagc
     4861 tgtcgctagt ggaaaagccg acttaggtgt atgtatttgt ggaactggag ttggtattaa
     4921 taatgctgtt aataaagttc caggaattcg ctcagctctg gtacgtgatt tgacatcagc
     4981 tatctatgct aaagaagaat taaatgctaa cgttatcgga tttggtggga aaattactgg
     5041 tgggctgtta atgacagata tcatcgaggc gtttataaga gctaaatata aaccaacaaa
     5101 agaaaacaaa gtattaattg aaaagatagc cgaggttgag actcataatg cccatcaaga
     5161 agagaatgac tttttcacag agttcttaga taaatggaat cgtggtgaat atcacgacta
     5221 aggagatatc tatgatttta acagtaactt tgaacccttc tattgatatt tcgtactgct
     5281 tagaaaactt taacatggat actgta
//
LOCUS       DATROQ010000109         5197 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.110, whole genome shotgun sequence.
ACCESSION   DATROQ010000109 DATROQ010000000
VERSION     DATROQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.110"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(36..1919)
                     /locus_tag="VBC94_001824"
     CDS             complement(36..1919)
                     /locus_tag="VBC94_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEO6980068.1"
                     /translation="MKKCFLAICLALSFFMVSVQADEVDYNIPHYEGNLTIHNDNSAD
                     FTEKVTYQFDSSYNGQYVTLGTAGKLPDNFDINNKPQVEVSINGKVRKVSYQIEDLED
                     GYRLKVFNGGEAGDTVKVNVQWKLKNVLFMHKDVGELNWIPISDWDKTLEKVDFWIST
                     DKKVALSRLWGHLGYLKTPPKIRQNNNRYHLTAFNVNKRLEFHGYWDRSYFNLPTNSK
                     NNYKKKIEHQEKIIERHGFILSFLLRILLPSFFIIVTLFISIRVFLFRKKVNKYGQFP
                     KDHHLYEAPEDLSPLELTQSIYSMSFKNFQDEEKKTHLISQEQLIQSILLDLIDRKVL
                     NYDDNLLSLANLDRASDAEIDFIEFAFADSTSLKPDQLFSNYQFSYKETLRELKKQHK
                     ASDLQNQMRRRGSNALSRITRLTRLISKDNINSLRRKGISSPYRKMSSEESKELSRLK
                     RFSYLSPLISFVVIIYTLFLNYFTYFCIYLLLFGVILLLNKIIFMMTRKISNGYIVTE
                     DGASRVYQWTSFRNMLRDIKSFDRSELESIVLWNRILVYATLFGYADRVEKVLRVNQI
                     DIPERFANIDSHRFAISVNQSSNHFSTITEDVSHASNFSVNSGGSSGGFSGGGGGGGG
                     GAF"
     gene            2055..3605
                     /locus_tag="VBC94_001825"
     CDS             2055..3605
                     /locus_tag="VBC94_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO6980069.1"
                     /translation="MKKILLSLFTALLITFGGMTSIQADEYLRVGMEAAYAPFNWTQN
                     DNTNGAVPIEGTDQYANGYDVQVAKKLAKKLNKKVVVVKTKWEGLVPALTSGKLDMII
                     AGMSPTEERKKEINFSKPYYISEPTLVVNAEGKYTNAKNISDFKNAKVTAQQGVYLYN
                     LIDQINGVKKEVAMGDFNQLRQAVESGVVDAYVSERPDATSAQTANPKLKMIELHQGF
                     KTSDADTNISVGMRKGDNRINQVNQVLESISRDKQIALMDKMIKEQPSVKKEKNGKPN
                     FFEQMATILKNNGSQFLRGTATTLLISMVGTIVGLFIGLLIGVFRTAPKSDNKLKAAL
                     QKLLGWLLNIYIEVFRGTPMIVQSMVIYYGTAQAFGVSLDRTLAAIFIVSINTGAYMS
                     EIVRGGIFSVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFILTFTVTRILRYIEKRFDSDNYT
                     TGANQLQV"
     gene            3615..4355
                     /locus_tag="VBC94_001826"
     CDS             3615..4355
                     /locus_tag="VBC94_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6980070.1"
                     /translation="MTQAILEIKHLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STFLRSINLLEEPSGGEILYHGHNVLEKGYDLNNYREKLGMVFQSFNLFENLNILENA
                     IVAQTTVLKRERQEAEKIAKENLNAVGMTEQYWKAKPKQLSGGQKQRVAIARALSVNP
                     EAILFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFAKEVSDRVIFMDKGI
                     IAEQGTPKQLFENPTQERTKEFLQRFLK"
     gene            4697..4927
                     /locus_tag="VBC94_001827"
     CDS             4697..4927
                     /locus_tag="VBC94_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980071.1"
                     /translation="MKLKISVLTAASAVTLLLATSASLAKADTVSYPSRDAGFIYSRG
                     YAPSESSYPSRDAGFIYSRGYAPKHFLSAYDK"
BASE COUNT         1750 a          963 c          824 g         1660 t
ORIGIN      
        1 taaatttttc tttattatac catacttttg gaaaattaga aggcaccgcc acctccgccg
       61 cctccgccgc ctgagaaacc acctgaagaa ccgcctgaat taacactaaa attagaagcg
      121 tgactaacat cttcagttat cgttgaaaaa tgattactag attgattgac tgaaatcgca
      181 aatcgatgac tatcaatgtt tgcaaatctt tctggaatat ctatttggtt cactctgagt
      241 actttctcaa cacggtcagc gtagccgaat aaagtagcgt aaaccaatat tcgattccat
      301 aatacgatac tttctaactc tgaacgatca aacgatttga tatcccttag catgttccta
      361 aaactagtcc attggtagac acgacttgct ccatcttcag ttacaatata accgttactt
      421 atttttcttg tcatcataaa aatgatttta ttcaacaaca ggataacacc aaacaataag
      481 agatagatac agaaataggt aaaataattt aaaaaaagcg tataaattat aacaacaaaa
      541 gaaataagag gtgataggta actgaatctt tttaacctag ataattcttt agactcttct
      601 gaagacattt tacgataagg ggatgaaatt ccctttcttc taagagagtt tatattgtct
      661 ttagaaatca accttgtgag acgcgtaatt cttgataagg cattacttcc tcggcgtctc
      721 atttgatttt gcagatctga agccttgtgc tgctttttca gttcacgtag tgtttcttta
      781 taactaaatt ggtaattaga aaagagttga tctggcttca aactcgtaga atccgcaaaa
      841 gcaaactcta taaaatctat ttctgcatca gaagctctat ctaagttagc tagagataac
      901 aagttatcat cataattcaa tacttttcta tcaatcaagt ctaatagaat tgactgtatg
      961 agttgttctt gactgataag gtgagttttc ttctcctcat cttgaaaatt tttaaagctc
     1021 atactataaa tgctttgagt taactctaat ggtgaaaggt cctcaggtgc ttcatataaa
     1081 tgatgatcct tagggaattg cccgtattta ttaacttttt ttctaaacag gaacacccta
     1141 attgagatga atagtgtcac aataataaag aatgaaggta ataatatcct taacaagaaa
     1201 cttaggataa aaccatgacg ctctattatc ttctcttgat gttcaatttt tttcttgtaa
     1261 ttatttttac tgtttgtagg tagattaaaa taagatctat cccaataacc atgaaattct
     1321 aatcgtttgt ttacattaaa agctgtcaaa tggtaacgat tattattttg tcttatttta
     1381 ggaggagttt taagataacc caagtgcccc caaagacgag aaagagcaac ctttttgtca
     1441 gttgatatcc aaaaatctac tttctctaac gttttatccc agtcgctaat aggaatccag
     1501 ttaagttcac caacatcctt atgcataaat agaacatttt ttagtttcca ctgaacattg
     1561 actttaacag tatcacctgc ttcaccacca ttaaacactt tcaaacggta gccatcctcc
     1621 aaatcttcta tctggtaact aactttcctt actttaccat taattgaaac ttcaacctgt
     1681 ggcttattat taatatcaaa attgtcaggt aacttacccg ccgtacctaa cgtgacatac
     1741 tgtccattat aggacgaatc aaattggtaa gtaaccttct ctgtaaaatc agcactatta
     1801 tcattgtgaa tagttagatt accctcataa tgaggaatgt tatagtccac ctcatctgct
     1861 tgaactgaaa ccataaaaaa actaagagct aaacaaatag ccaaaaaaca tttcttcatt
     1921 atcatcctcc ctcctttgtt tgtataccat tttaacataa aaatacaatt aatcgctttc
     1981 aataagataa tgatattgaa atcgaccttg tttttcggta aaataggagg aagtaactta
     2041 tcggaggctg ttgaatgaaa aaaatattac taagtttatt cactgctttg cttatcacct
     2101 ttggaggtat gacaagtata caagcagatg aatatctacg tgtcggtatg gaggcagcct
     2161 atgcgccttt caactggaca caaaatgata acacaaatgg tgcagttcct attgaaggaa
     2221 ctgatcaata tgctaatggt tatgatgtac aagtcgctaa aaagttagct aaaaagttaa
     2281 ataaaaaagt tgttgttgtc aaaacaaaat gggaaggcct tgttccagca ctaacttctg
     2341 gaaaactcga tatgattatt gcaggaatga gtcctaccga agaacgtaaa aaagaaatta
     2401 atttttcaaa gccttactac atttcagaac ctactctagt tgttaacgct gaaggaaaat
     2461 atactaatgc taaaaatatt agtgatttca aaaatgctaa agtaactgcc caacaaggag
     2521 tctacttata caacctcatt gatcaaatca atggtgtcaa aaaagaagtt gccatggggg
     2581 actttaatca attaagacaa gctgtagaat ctggtgttgt agacgcctat gtttcagaac
     2641 gtccagatgc aacttccgct caaactgcta atcctaaatt aaaaatgatt gaactgcacc
     2701 aagggtttaa aacatctgat gccgatacaa atatttccgt tgggatgcgt aaaggtgaca
     2761 atcgtattaa tcaagtcaat caagttctcg aaagcatttc tagagataaa caaatcgcct
     2821 tgatggataa gatgattaaa gaacaaccat ccgttaaaaa agaaaaaaat ggaaaaccaa
     2881 acttctttga acagatggca actattttaa aaaacaatgg ttctcaattc ctcagaggaa
     2941 cagctacaac cctcctaatt tctatggtag gaactatcgt cggattattt atcggccttc
     3001 tcatcggtgt tttccgtaca gcaccaaaat ctgacaataa gttaaaggct gccttacaaa
     3061 aattattagg ttggttactt aacatctata ttgaggtctt ccgcgggaca cctatgattg
     3121 tacaatcaat ggttatctac tacggaacag cacaagcttt cggagtttcg ctggaccgta
     3181 cactagcagc tatatttatt gtttcaatca atactggtgc ttatatgagc gaaattgtac
     3241 gtggtggtat tttctctgtt gataaaggac aatttgaagc cgcaactgca cttggtttca
     3301 cccatggaca aaccatgcga aaaattgttc taccacaagt tgtacgtaac atactcccag
     3361 ctactggtaa tgagtttgtt atcaatatca aagatacctc tgtactgaac gttatctctg
     3421 ttgtcgaact ctacttctct ggtaatactg ttgcaacaca aacctaccaa tatttccaaa
     3481 cattcaccat tattgcgatt atttacttca tcttaacctt caccgtaaca cgtatcttac
     3541 gttacattga aaaacgtttt gattcagata attacacaac cggtgcgaat caattacaag
     3601 tctaggaggt tggtatgaca caagctattt tagaaattaa acatttgaaa aaatcttacg
     3661 gaagcaatga agtgttaaaa gacatttcgc tttcggtcaa caaaggcgaa gttatttcta
     3721 tcattggttc ttctggatca ggaaaatcaa ctttcctacg ttctattaac ctcttagaag
     3781 aaccaagtgg aggagaaatc ttatatcacg gtcacaatgt tcttgaaaaa ggctatgatt
     3841 taaataatta tcgtgaaaaa cttgggatgg tctttcaatc ttttaacctc tttgagaatt
     3901 taaacatctt agagaacgct attgttgcac agactactgt tctaaaacgt gaacgccaag
     3961 aagctgaaaa gattgctaaa gaaaacttaa atgctgttgg catgactgag caatattgga
     4021 aagctaaacc aaaacaatta tcaggtgggc aaaagcaacg tgtcgcaatc gcccgagcac
     4081 tttctgttaa cccagaagct atactttttg atgaaccaac ttcagccttg gatccagaaa
     4141 tggttggtga ggtccttaaa acaatgcaag atctagctaa atctggatta acaatgataa
     4201 ttgtgacaca cgagatggaa tttgctaaag aagtatcaga ccgcgttatt ttcatggaca
     4261 aaggaatcat tgcagagcag ggtactccta agcaactttt cgaaaatcca acacaagaaa
     4321 gaactaagga atttctacaa cgcttcttaa aataaaattt atccacattt aaatatagac
     4381 tatttatacc cctacaggtt ttgtcattgt catcattaca atggaactgg aggggatttt
     4441 ttattaccac atctttgatg aaatctacga ctagcctcaa ctatagatca aattagacag
     4501 tcgttattgg attgtatact attaaataag ttactaaatc tcatttttta attttaatcg
     4561 atatttaatt aattatatat ttgtttaaaa ttaaaaacat gttgttttaa tgagcttttg
     4621 atgatataat atagtatgca caaaatgaac taaccactgt gcaagaatag tttattatct
     4681 ttgaaaggat tttataatga aacttaaaat cagtgtttta acagcagctt cagctgtcac
     4741 tctattatta gctacttcag caagtcttgc gaaggcagac acagtgtctt atccttcacg
     4801 tgatgccgga tttatctact ctcgtgggta tgcgccttct gaatcaagct atccttcacg
     4861 cgatgccggg tttatctact ctcgtggata cgctccaaaa cacttcctaa gtgcttacga
     4921 taaataaaaa ctgaccttat taacttaacc gatacacttt cacaagtata aattttcatt
     4981 caattacatt aaaaataatt atttagtttg ctgaaaaacg atcactcatc gatcgttttt
     5041 ttatatgcac aatgatagcc attagctact ttctcgttgg gtattttcct cattcatctt
     5101 tcacttaaca tcacttgtca aatctaaacc tcattaataa aggtttaata tatctttgaa
     5161 cttattactt ttgattgata tttaattact ttgctat
//
LOCUS       DATROQ010000110         4998 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.111, whole genome shotgun sequence.
ACCESSION   DATROQ010000110 DATROQ010000000
VERSION     DATROQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.111"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            1..249
                     /locus_tag="VBC94_001828"
     CDS             1..249
                     /locus_tag="VBC94_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEO6980072.1"
                     /translation="MEVKTLANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANP
                     SEELYRAASSSIDKAASKGLIHTNKASRDKARLATKLG"
     gene            complement(333..1166)
                     /locus_tag="VBC94_001829"
     CDS             complement(333..1166)
                     /locus_tag="VBC94_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6980073.1"
                     /translation="MLTHKNILLTIIFGLFMIILSACGMSNKEMAGIDNWEHYQKEKK
                     ITIGFDNTFVPMGFESRSGDYTGFDIDLANAVFKEYGISVKWQPINWDMKETELNNGN
                     IDLIWNGYSKTAERAKKVAFTNPYMNNHQVIVTKTSSHINSIKDMKGKKLGAQSGSSG
                     FDAFNAKPDILKKFVKGKEAVQYDTFTQALIDLKNNRIDGLLIDEVYANYYLKQEGNI
                     KAYYFVKTAYQGENFVVGARKVDRRLIEKINKAFKQLHNKGRFQKISYKWFGEDVYSK
                     E"
     gene            complement(1180..1809)
                     /locus_tag="VBC94_001830"
     CDS             complement(1180..1809)
                     /locus_tag="VBC94_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6980074.1"
                     /translation="MLELKNISKCYGQKEIFKDFNLTVEEGKILSLVGPSGGGKTTLL
                     RMLAGLEKIDSGTIVHDGKEVSVDHLETLNLLGFVFQDFQLFPHLTVLDNLILSPVKT
                     MGLSKELAKEKALVLLERLGLKDHALVYPFSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQEVEKLILQNRETGMTQIVVTHDLQFAESISDTILKINPK"
     gene            complement(1819..2460)
                     /locus_tag="VBC94_001831"
     CDS             complement(1819..2460)
                     /locus_tag="VBC94_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6980075.1"
                     /translation="MSYILEILPSLLVGAQVTLQVFFIVICLSIPLGIVVAFLMKVHF
                     KPLQWLLTTYIWIMRGTPLLLQLIFFYYFLPSVGISFDRMPAAIIAFTMNYAAYFAEI
                     FRGGIESIPKGQYEAAKVLKFSKFQTVWYIVLPQVFKIVLPSVFNETITLVKDSSLVY
                     ILGVGDLLLESKTAANRDATLAPMFIAGGIYLLLIGLLTILSKQVEKRFNYYK"
     gene            complement(2595..2924)
                     /locus_tag="VBC94_001832"
     CDS             complement(2595..2924)
                     /locus_tag="VBC94_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEO6980076.1"
                     /translation="MSLPNCPKCNSEYVYEDGILLVCPECAYEWNPEEIEEEVGLIVL
                     DSNGTRLSDGDTVTVIKDLKVKGAPKDIKQGTRVKNIRLVDGDHNIDCKIDGFGAMKL
                     KSEFIKK"
     gene            complement(3087..4901)
                     /gene="glmS"
                     /locus_tag="VBC94_001833"
     CDS             complement(3087..4901)
                     /gene="glmS"
                     /locus_tag="VBC94_001833"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEO6980077.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVVGDNKSQL
                     VKSVGRIAELQAKVGDSVSGTTGIGHTRWATHGKPTEGNAHPHTSGSGRFVLVHNGVI
                     ENYLQIKETYLTKHNLKGETDTEIAIHLVEHFVEEDNLSVLEAFKKALHIIEGSYAFA
                     LIDSQDADTIYVAKNKSPLLIGLGNGYNMVCSDAMAMIRETSEYMEIHDKELVIVKKD
                     SVEVQDYDGNVIERGSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRKLISTYANESG
                     DMNVDSDIIKSVQEADRLYILAAGTSYHAGFAAKTMIEKLTDTPVELGVSSEWGYNMP
                     LLSKKPMFILLSQSGETADSRQVLVKANEMGIPSLTITNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQVATLAFLAKAVGEANGKAEAKDFDLVHELSIVAQSIEATLSEKD
                     VISEKVEQLLISTRNAFYIGRGNDYYVTMEAALKLKEISYIQTEGFAAGELKHGTISL
                     IEDNTPVIALISADSTIAAHTRGNIQEVVSRGANALIIVEEGLEREGDDIIVNKVHPF
                     LSAISMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
BASE COUNT         1637 a          987 c          728 g         1646 t
ORIGIN      
        1 atggaggtga aaacattggc aaatattaaa tcagctatca aacgcgctga acttaacgtt
       61 aaacaaaacg aaaaaaactc agcacaaaaa tcagctatgc gtactgctat taaagcattt
      121 gaagctaacc cttcagaaga actttaccgc gctgcttctt caagcatcga caaagctgct
      181 tctaaaggtt taattcacac aaacaaagca agccgcgata aagcacgtct tgcaactaaa
      241 cttggttaat taacctagaa aacatcggat atatcgatgt tttttattta attttttcgt
      301 agaataaaag actaggagaa attcctagtc ttttattctt tactataaac atcttcacca
      361 aaccatttgt aagagatttt ttgaaatctc cccttattat gaagctgttt gaaagctttg
      421 ttaatctttt caattagtct acgatcaact ttacgagctc ctactacaaa attttctcct
      481 tgataagcag ttttaacaaa ataataagct tttatatttc cttcttgctt taaataatag
      541 ttagcataaa cttcatcaat caaaagacca tcaatacggt tattttttaa atcaatcaaa
      601 gcctgagtga aagtatcgta ttgaactgct tcttttcctt ttacaaactt ttttaaaata
      661 tcaggtttag cgttaaaagc atcaaaacca gatgaacccg actgggctcc tagttttttc
      721 cccttcatat ccttaatact attaatatgt gatgaagttt tagtaacaat tacttgatga
      781 ttattcatat atgggtttgt aaaagcgact tttttagcac gttctgccgt ttttgaataa
      841 ccattccaaa taaggtctat attaccatta ttaagttcag tttctttcat atcccagtta
      901 ataggctgcc atttcactga aataccgtat tctttaaaaa cagcattagc taaatcaata
      961 tcaaagccgg tatagtcacc agaacgactt tcaaatccca taggaacaaa agtattatca
     1021 aatccaatag taattttctt ttccttttga taatgttccc aattatcaat accagccatt
     1081 tccttattag acataccaca tgctgataat ataatcataa ataatccaaa tataatggtt
     1141 aataatatat ttttgtgagt caacaatcta cccccaagtc tacttaggat taattttgag
     1201 aatcgtatca gatatacttt cagcaaattg aagatcatgt gttactacaa tttgtgtcat
     1261 acctgtttct ctattttgta aaattagttt ttctacttct tgacgcaact ctggatcaag
     1321 agcgctagtt ggctcatcat aaccaataat ctgtggatca atcatcatcg cacgagctag
     1381 tgcgacacgt tgcttttgcc ccccagataa tgaaaatgga tagactaaag catggtcttt
     1441 aagtcccaag cgttcaagta atactaaggc tttttcttta gcaagttctt ttgataatcc
     1501 catcgtttta acaggagaga ggatcaaatt atctaatact gttaaatgtg ggaataattg
     1561 aaagtcttgg aagacgaatc ctaaaagatt tagtgtttcc aaatgatcaa cagatacttc
     1621 tttaccatcg tgaacaatag tccctgaatc aatcttttcc aatccagcta acatacgtaa
     1681 tagtgttgtt tttccacctc cagaaggtcc tactaaagag agaattttac cttcttctac
     1741 agtcagattg aaatctttaa agatttcttt ttgcccatag catttagaaa tattttttaa
     1801 ttctaacatc gtccctcctt atttatagta gttaaaacgc ttttcaactt gtttagataa
     1861 aatagttaaa agaccaatta ataataggta aataccacct gctataaaca ttggtgcaag
     1921 tgttgcatcc ctatttgcgg ctgtcttact ttctagaagt aagtcgccca caccaagtat
     1981 gtaaaccaat gaggaatctt tcactagagt aatcgtttca ttgaaaacac tcggtaagac
     2041 tattttgaag acttgaggta atactatgta ccaaaccgtt tgaaacttag aaaactttaa
     2101 tacctttgca gcttcatatt gccctttagg aatagactca atacctcctc gaaaaatctc
     2161 tgcaaaataa gctgcataat tcatcgtaaa agcaataatc gcagctggca tacgatcaaa
     2221 agaaattcct acacttggta aaaagtaata aaaaaagata agctgtaata acagaggcgt
     2281 gcctcgcata atccaaatat aagttgtcag tagccattga agcggcttaa aatgaacttt
     2341 cattaggaag gctacaacaa taccaagtgg aattgataaa caaataacaa taaagaaaac
     2401 ttgcaatgtc acttgcgcac ctactaataa gctaggtaag atttctaaaa tataagacat
     2461 ggtcccccct aaataaaaaa tagatgttct cattatatca aaattcaaac caatgtcata
     2521 gagctttttt aataaaaatg catgaaatca agtcatattc aaaaagcaat tgaccgtcgc
     2581 caattgcctg aaagctattt ttttataaac tcagatttta gtttcatagc tccaaaacca
     2641 tcaattttac aatcgatatt atgatcgcca tcaactaaac gaatattttt aacacgagtg
     2701 ccttgcttaa tatccttagg cgctcctttt acttttaaat ccttaataac tgtcacagta
     2761 tctccatctg ataagcgcgt tccattgcta tccaatacaa ttaaacctac ttcttcttcg
     2821 atttcttctg gattccactc gtaagcacat tctggacaaa ccagtaaaat accgtcttca
     2881 tagacatatt cagagttaca cttagggcaa tttggtaatg acatttttct ctccttaaat
     2941 taattaataa aatagtcagc aagtaacatt ctaactgatt ataggcttct tgacaatagg
     3001 aaaaagccta ttttccttca aaaataggct tttttacaat ttttaatatt ttgtagttaa
     3061 tattagcgta ttttagtgta acgagtctat tcaacagtta cagcttttgc caaattacgt
     3121 ggtttatcaa catctaagcc acgttgtaat gaagcatagt aagcaattaa ttgagtcgga
     3181 ataaccattg aaatggctga taagaatggg tgaactttat taacaataat atcatcacct
     3241 tcacgttcca acccttcttc tacaataatc aaagcatttg ctccgcgaga aacaacttct
     3301 tggatatttc cacgagtatg cgctgcaata gttgaatcag cagatattaa agcaataaca
     3361 ggagtattgt cttcaatcaa cgaaattgta ccatgtttta attcacctgc agcaaaacct
     3421 tctgtctgaa tataagaaat ctcttttagc ttcagtgcag cctccattgt aacataatag
     3481 tcgttaccac gaccaatgta gaaggcatta cgtgtcgaga ttaacaactg ctctaccttc
     3541 tcagagataa catctttttc tgataaggtc gcttcaattg attgtgctac aattgacaat
     3601 tcatgaacca aatcaaaatc ttttgcttct gccttaccat ttgcttctcc taccgctttt
     3661 gctaagaaag ctaacgttgc aacttgagca gtatatgctt ttgtagaagc aacagcgatt
     3721 tcaggtcctg catgaatgag catcgtatag gttgcttcac gagaaagtgt tgagccaggc
     3781 acatttgtta tagttaaact agggattccc atttcgtttg ctttaactaa aacttgtcta
     3841 ctatctgctg tttctcccga ttgactaagt aaaataaaca ttggcttctt actcaaaagt
     3901 ggcatgttgt aaccccattc tgaagaaact ccaagttcaa caggtgtatc agttaatttt
     3961 tcgatcatgg tcttagcagc aaaaccagca tgatatgacg ttcctgcagc caatatgtaa
     4021 agacgatctg cttcttggac tgattttata atatcagaat ccacattcat atctccagat
     4081 tcgttcgcat aagttgaaat caacttacgc atcacagttg gttgctcatc aatttcttta
     4141 agcatgtaga atggataagt ccccttacca atatctgata agtcaagctc tgctgtgtag
     4201 ctaccacgtt caatgacatt accatcataa tcttgaactt caacgctatc ttttttaaca
     4261 ataacgagtt ctttatcgtg gatttccata tattcacttg tctcacgtat catagccatg
     4321 gcatctgaac acaccatatt ataaccattt cccaaaccta ttagaagagg tgatttattt
     4381 ttagctacgt aaatcgtatc agcatcttga gaatcaataa gagcaaatgc gtatgatcct
     4441 tctataatat gtaaggcttt tttaaaggct tcaagcactg ataagttatc ttcttcaaca
     4501 aagtgttcaa ctaaatgaat agctatttct gtatcagttt cacccttgag gttatgtttt
     4561 gttaagtaag tttctttaat ttgaaggtaa ttttcaataa caccattgtg taccaataca
     4621 aaacgaccag atcctgaagt gtgaggatgt gcatttcctt cagtaggttt accatgggtt
     4681 gcccaacgtg tatgaccgat acctgtcgta ccactaacag aatctcctac ttttgcctga
     4741 agctccgcga ttcgaccgac tgacttcaca agttgacttt tgttatcacc tactacaaaa
     4801 atacctgctg agtcatatcc tcggtactct agcttttcta agccttgaat taaaatatcc
     4861 gttgcatttg tatttccaac tacacctacg ataccacaca tattattatt accaataaga
     4921 gctttctctt atactttcta tctaaacttt ctttttgaat tggtatatat taatatatac
     4981 cgttaaaaag taacaaaa
//
LOCUS       DATROQ010000111         4960 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.112, whole genome shotgun sequence.
ACCESSION   DATROQ010000111 DATROQ010000000
VERSION     DATROQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.112"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..1301
                     /locus_tag="VBC94_001834"
     CDS             <1..1301
                     /locus_tag="VBC94_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6980078.1"
                     /translation="DTKFGKYRPILIIGNVITALSLIFLLALRGVDENIRFPLFILVL
                     IIHKIGYSMQQTITKAGQTALTNDPKQRPIFNIVDAVMTTSLMTGGQFVVSVFLVPKF
                     GNFTPQFFNVLIFGTILISAILAIVAIIGIWAKDRKEFFGLGENTQKTALKDYWKVLK
                     GNKPLQILSIAAALVKFAIQFFGDSVVMVLLFGILFGNYALSGQFSLLFIVPGVIINI
                     LFSTIARKKGLRFSYVRAIQIGMIGLLAFGAVLYVGKPGDLSLTSLNLYTILFIVTNI
                     IARYASQAPASLVLTMGADISDYETSESGRYVSGMIGTIFSLTDSIASSFAPMVVGFV
                     LAGIGFSKSFPTIETPLPPDLKMAAISILVAIPFIALSIALLLMKFYKLDKEEMVRIQ
                     EKIQVMKAATTKERAKDIARNVPLTDMDYVDVTKYPIDKD"
     gene            1368..2393
                     /locus_tag="VBC94_001835"
     CDS             1368..2393
                     /locus_tag="VBC94_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO6980079.1"
                     /translation="MAKIISLGEVLLRLSPPQYHTLMQANHLKCQFGGSELNVLASLA
                     QLGYHVGLVSALPDNDLGKMASQFILSQQISPAAIIKKEGRLGIYYYEQGFSVRTNKV
                     IYDRNYSSFWESTLSDYDFTSIFKGVDWFHVSGITPALTKDLYEVTRFLMTKAKEGGV
                     KVSIDLNFRESLWSSFQEAREQLSPLLGLLDVCFGLEPIYLAGESEDLKDELGLSRPY
                     LDIELLEKITQKIVQEYGLDYIAFTQREMEYTNQYMLKSYLYHNNMLYQTDKTGVEVL
                     DRVGTGDAFAAGLIHALLEKETPQRALEIAMATFKYKHTIQGDINIMTRDDIAYLIEK
                     ETNDIKR"
     gene            2410..4209
                     /gene="uidA"
                     /locus_tag="VBC94_001836"
     CDS             2410..4209
                     /gene="uidA"
                     /locus_tag="VBC94_001836"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEO6980080.1"
                     /translation="MLYPLLTKTRNTYDLGGIWNFKLGEHNPNELLPSDEVMVIPTSF
                     NDLMVSKEKRDYIGDFWYEKVIEVPKVSEDEEMVLRFGSVTHQAKIYVDGVLVGEHKG
                     GFTPFEVLVPECKYNNEKIKVSICANNVLDYTTLPVGNYSEIIQEDGSIKKKVRENFD
                     FFNYAGVHRPLKLMIRPKNHIFDITITSRLSDDLQSADLHFLVETNQKVDEVRISVFD
                     EDNKLVGETKDSRLFLSDVHLWEVLNAYLYTARVEIFVDNQLQDVYEENFGLREIEVT
                     NGQFLLNRKPIYFKGFGKHEDTFINGRGLNEAANLMDLNLLKDMGANSFRTSHYPYSE
                     EMMRLADRMGVLVIDEVPAVGLFQNFNASLDLSPKDNGTWNLMQTKAAHEQAIQELVK
                     RDKNHPSVVMWVVANEPASHEAGAHDYFEPLVKLYKDLDPQKRPVTLVNILMATPDRD
                     QVMDLVDVVCLNRYYGWYVDHGDLTNAEVGIRKELLEWQDKFPDKPIIITEYGADTLP
                     GLHSTWNIPYTEEFQCDFYEMSHRVFDGIPNLVGEQVWNFADFETNLMILRVQGNHKG
                     LFSRNRQPKQVVKEFKKRWMTIPHYHNKKNSVK"
     gene            4238..4909
                     /locus_tag="VBC94_001837"
     CDS             4238..4909
                     /locus_tag="VBC94_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEO6980081.1"
                     /translation="MARPLVEQTADRLLHLILEREYPVGAKLPNEYELAEDLDVGRST
                     IREAVRSLATRNILEVRQGSGTYISSKKGVSEDPLGFSLIKDTDRLTSDLFELRLLLE
                     PRIAELVAYRITDDQLQLLEKLVGDIEDAVHAGDPKHLLLDVEFHSMLAKYSGNIAMD
                     SLLPVINQSIHLINANYTNRQMKSDSLEAHREIIKAIREKNPVAAHDAMLMHIMSVRR
                     SALKA"
BASE COUNT         1587 a          763 c          995 g         1615 t
ORIGIN      
        1 ctgatacaaa atttggtaag tatcgtccta ttttgattat tgggaacgtt attacggctt
       61 tgtcattgat ttttcttctt gcattgagag gtgtcgatga aaatattcgt ttcccattat
      121 ttatccttgt tctaattatc cataaaattg gatactcaat gcaacagaca ataactaaag
      181 cagggcaaac agcactgaca aatgacccta agcaacgtcc tatttttaat atcgttgatg
      241 cagttatgac aacatcatta atgacaggcg gtcagtttgt cgtttcagtt ttcttagtac
      301 caaaatttgg taattttaca cctcaattct tcaatgtcct tatttttgga acaattctta
      361 tttcagctat tttagcaatc gttgcaatta ttgggatttg ggcaaaagac agaaaagaat
      421 tctttggttt aggagagaat acccaaaaga ctgctctaaa agattactgg aaggtgttga
      481 aaggaaataa accgcttcag atattatcca ttgctgcagc tttggtaaaa tttgccatcc
      541 aattctttgg cgattccgtt gttatggtgc tattgtttgg tattttattt ggtaattatg
      601 ctttatcagg acaattctca cttcttttca ttgttcctgg tgttattatt aatattttgt
      661 tctcaacaat tgctcgtaaa aaaggtcttc gtttttcata tgtcagagca attcagatcg
      721 gtatgattgg tttacttgct tttggtgcag ttctttacgt tggtaagcct ggtgacctta
      781 gtttaacaag cctaaatctt tatactattt tatttatcgt tactaatatt attgcgagat
      841 acgcttcaca agcacctgct agtttagtat tgactatggg agcagatatt tctgactatg
      901 aaacttctga atccggtcgt tatgtatcag gaatgattgg aactatcttc tccttaacag
      961 attcgattgc ttcttcattt gctcctatgg tagtaggttt tgtgttagca ggaattggat
     1021 tttcaaaatc atttccaact atcgaaacac cactgccccc tgatttaaaa atggcagcta
     1081 ttagtatcct tgtggccatt ccatttatag ctttgtctat agctcttctt ttaatgaaat
     1141 tttacaaact agataaagaa gaaatggttc gtattcaaga gaagattcaa gttatgaaag
     1201 cagcaactac taaggaacgt gctaaagata ttgctcgtaa tgttcctttg acagatatgg
     1261 attatgttga tgtaacaaaa tatcctattg ataaagatta aaaagtttag ctgctcttca
     1321 atagagcagc tattttgtta aataagagct accattggag gattcttatg gcaaaaatta
     1381 tttcattagg tgaggttctt ttgcgattat ctccacctca atatcacaca ctgatgcagg
     1441 ctaatcacct aaaatgccaa ttcggtggtt cagaacttaa tgtcttagca agccttgctc
     1501 aactaggtta ccatgtagga ttggtctcag ctttgcctga taatgatttg ggcaagatgg
     1561 caagtcaatt tatattgtca cagcaaatta gtccagctgc tataattaaa aaagaaggac
     1621 gactaggaat ttattactat gaacaaggat tttcggtaag aacaaataag gttatctacg
     1681 atcgtaacta ttcatctttt tgggaaagta ccttgagtga ttatgatttt acttctattt
     1741 ttaagggagt ggattggttt catgtttctg gaattactcc agcactaacc aaggacttat
     1801 atgaggtgac tcggttcctt atgacaaaag ctaaagaagg aggtgtaaaa gtatcgattg
     1861 atctcaattt tcgtgagagt ttatggtcat cttttcaaga ggcacgtgag caattatcgc
     1921 cactattagg attattagat gtttgctttg gcttagagcc gatttattta gctggcgaaa
     1981 gtgaagattt aaaagatgag ttgggcttat ccagacctta ccttgatatt gaattacttg
     2041 aaaagataac ccaaaagatt gtccaagaat atgggttaga ctatattgcc tttactcaaa
     2101 gggaaatgga atatactaat cagtatatgt taaaatcata tttgtatcat aataatatgt
     2161 tatatcaaac tgataaaact ggcgttgaag tattagatcg tgtggggaca ggagatgcct
     2221 ttgctgcagg tttaattcat gcgcttctgg aaaaagaaac tcctcaaagg gccttagaaa
     2281 tagctatggc tacatttaaa tacaaacata ccattcaagg agatatcaat atcatgacac
     2341 gagatgatat tgcttacctc attgaaaaag aaacaaatga tattaaacga tagtgtaaaa
     2401 ggagagtgta tgttatatcc attattgaca aaaacaagaa atacttatga tttaggcggt
     2461 atttggaact ttaaattagg agaacataat ccaaatgaat tactaccttc agatgaagtc
     2521 atggttatcc cgacttcatt taatgatttg atggtaagta aagaaaaacg tgattatata
     2581 ggtgattttt ggtatgaaaa agtcattgaa gtacctaaag tatcagagga tgaagagatg
     2641 gtgctgcgtt ttggctcagt gacacatcaa gctaaaattt atgttgacgg tgtattggta
     2701 ggagagcata agggaggttt tactcctttt gaagttcttg ttcctgaatg taagtataat
     2761 aatgagaaaa ttaaggtatc aatttgtgct aataacgttt tagactatac cacgcttccg
     2821 gtcggaaatt atagtgaaat cattcaagaa gatggtagca ttaagaagaa agtacgtgag
     2881 aattttgatt tcttcaatta tgcaggtgtg catcgtcctc ttaagttgat gatacgtcct
     2941 aaaaatcata tttttgatat tacaattaca agtcgtctat cagatgattt acagtcagca
     3001 gatttacatt ttttagtgga gactaatcaa aaggttgacg aagttagaat tagcgttttt
     3061 gatgaagata acaaactagt gggtgaaacg aaggatagca gattatttct tagtgatgtc
     3121 catctttggg aagttttaaa tgcctatctg tacacagcgc gtgttgaaat ttttgttgat
     3181 aatcaactcc aagatgtcta tgaggaaaat tttggtctta gagaaataga agtgacaaat
     3241 ggtcaattcc tattgaatcg caaacctatt tattttaaag gatttggtaa acatgaagat
     3301 acgttcatta atggcagagg tttgaatgaa gctgctaact taatggattt gaatcttttg
     3361 aaggatatgg gggcaaactc ttttagaaca tcccattatc cttattctga agaaatgatg
     3421 cgtttagcag atcgaatggg agtgttagtg atagatgagg ttccagcagt agggttattt
     3481 caaaatttta acgcttcctt agatttgtca ccaaaagata atggtacgtg gaatttgatg
     3541 caaacaaaag cggcgcatga acaagctatc caagaattag tgaagcgtga taaaaatcat
     3601 cctagcgtcg tgatgtgggt agtcgctaac gaaccggcta gtcatgaagc gggagcacat
     3661 gattattttg agccattagt aaaactttat aaagatttag accctcaaaa acgtcctgtc
     3721 accttggtta atattttaat ggcaacccca gatagagacc aagtgatgga cctggttgat
     3781 gttgtctgcc ttaatcgtta ctatggttgg tacgttgacc acggtgattt aacaaatgca
     3841 gaagtaggta taagaaaaga gttattagaa tggcaagata aatttcctga caaaccaatt
     3901 atcataacgg agtatggcgc tgatacgtta ccgggattac attctacttg gaatattcca
     3961 tatacagaag aatttcaatg tgatttttat gaaatgagtc atcgtgtttt tgatggtatt
     4021 cctaatttag ttggtgagca agtctggaat tttgcagact ttgaaactaa tctgatgata
     4081 cttcgtgtac aggggaatca caaaggtctc ttttcaagga atcgccagcc gaaacaagtc
     4141 gtcaaagaat ttaaaaaacg ctggatgact attcctcatt accataataa aaaaaatagt
     4201 gtaaaatagg ggtagttatt ttagaagagg tgatgatatg gctagaccct tagtagaaca
     4261 aacagcagat cgtctattgc atttaatttt agaaagagaa taccctgttg gtgctaagct
     4321 ccccaatgaa tatgaattag cagaagatct agatgttggt cgcagcacaa tccgagaagc
     4381 agtcagaagt ttagcaacgc gtaatatttt agaagtcaga cagggctctg gaacatatat
     4441 tagttctaaa aagggtgttt ctgaggatcc attagggttc tctctaataa aggacacaga
     4501 taggttgaca tcagatctct ttgaactacg ccttttattg gaacctcgaa tagcagagct
     4561 ggtagcttat agaattacag atgatcagtt acaattactc gaaaagcttg ttggagatat
     4621 tgaagatgct gttcacgcag gcgatccgaa acatttactt ttagacgtgg aatttcattc
     4681 catgctagct aaatacagtg gtaatattgc aatggatagc cttttgccag tcatcaacca
     4741 gtcaattcat ctgataaatg cgaattatac aaatcgtcag atgaagtcgg atagtttaga
     4801 agcacataga gaaattatca aagcaatccg agaaaaaaat ccagtagctg cacatgatgc
     4861 tatgcttatg catatcatga gtgttagacg ttcagcgttg aaagcttaat ataagaagaa
     4921 aaatagctat tcttgaatag ctattttttt agtattcttc
//
LOCUS       DATROQ010000112         4851 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.113, whole genome shotgun sequence.
ACCESSION   DATROQ010000112 DATROQ010000000
VERSION     DATROQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.113"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..1223)
                     /locus_tag="VBC94_001838"
     CDS             complement(<1..1223)
                     /locus_tag="VBC94_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6980082.1"
                     /translation="MFYLKIAWHNLKHSIDQYIPFLLASLLLYSLTCSTLLILMSAVG
                     RDMGTAATVLFLGVIVLSIFAVVMEHYSYNILMKQRSSEFGLYNILGMNKRQVARVAS
                     LELFIIYIFLISIGSLFSAFFAKFIYLIFVNIINYHALNLSLSLWPFIICIVIFTGIF
                     LTLEVPVIRHVHLSSPLSLFRKKQQGEKEPKGNLILAILALVAIAIAYTMALTSGKAP
                     ALAVIYRFFFAVLLVIAGTYLFYISFMTWYLKRLRQNKHYYYKSEHFVSTSQMIFRMK
                     QNAVGLASITLLAVMALVTIATTVSLYSNTQNVVTGLFPKSVSLSIDNSKGDAKNIFE
                     EKILKKLGKSSKEAITYNQTMISMPVSQSSELNITSKNVKHVDITKTGFMYLITQNDF
                     RRLGHQLPKLKDNQVA"
     gene            complement(1225..1977)
                     /locus_tag="VBC94_001839"
     CDS             complement(1225..1977)
                     /locus_tag="VBC94_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6980083.1"
                     /translation="MLLEINHLEKVFRTRFSKEETRALQDVDFKVEQGEFIAIMGESG
                     SGKTTLLNILATLEKPTNGQVILNGEDITKIKEAKLASFRLKNLGFVFQDFNLLDTLS
                     VRDNIYLPLVLDRKRYKEMDHRLSELSSHLRIDDLLDKRPFELSGGQKQRVAIARSLI
                     TNPQILLADEPTAALDYRNSEDLLNLFETINLDGQTILMVTHSANAASHAKRVLFIKD
                     GRIFHQLYRGNKNNSEFNKDISLTMSAILGGE"
     gene            complement(2003..2965)
                     /locus_tag="VBC94_001840"
     CDS             complement(2003..2965)
                     /locus_tag="VBC94_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEO6980084.1"
                     /translation="MRRKIVLLFVVPMLIVLGILGVVVHYYGSALNIYLLPPSSERYG
                     RVILDRVEQRGLYSQGRQWQIIRQRSEKKLKTSKSYQESRNIVQEAVRYGGGKHSQIL
                     SKETVRRDTLDSRYPEYRRLNEDILLITIPSISKLDKRSISHYSGKLQNILMEKSYKG
                     LILDLSNNTGGNMIPMIGGLASILPNDTLFHYTDKYGNKKTITMKNIPLEALKISRKT
                     INTKHVPIAIITNHKTASSAEMTFLSFKGLPNVKSFGQATAGYTTVNETFMLYDGARL
                     ALTTGIVSDRQGYKYENTPILPDQVTSLPLQESQSWLKSRINQN"
     gene            3131..3619
                     /locus_tag="VBC94_001841"
     CDS             3131..3619
                     /locus_tag="VBC94_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEO6980085.1"
                     /translation="MAKTSKKLTKSLGPIGKLISIIPDTTELIGKAIDNSRPIIEKEL
                     DRRHEKKQTYTQLDNVVHLDINDAQVYLEQRHFNVSRIVAEPNSKWATLHPNQVVNMS
                     PKAGRVRIGSLVKLYYITEDILEESKILLLSKSEKSKARQQRLTEVLSTPSQLLKKNR
                     NK"
     gene            complement(3651..4499)
                     /locus_tag="VBC94_001842"
     CDS             complement(3651..4499)
                     /locus_tag="VBC94_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO6980086.1"
                     /translation="MKIKKLLGLTTTVVISALILGACGQSKNEDAKVVRVGTMVKSKT
                     EKARWDKIEELVKKKGVKLKFTEFTDYTQPNKALESDEIDINAFQHYNYLNNWNKANK
                     TNLVSVAETYFTSFRLYSGTKNGKGKYQTVSEIPNKATITIPNDAVNESRSLYLLQSA
                     GLLKLKVSGDTLATMSDVVSNPKSLDLKEVDAAQTARSLDSTDAAVINNDFVTEAGIN
                     PKSAIFIEPKSKNAKQWYNLLVAQKGWQDKSKAKAIKEVVKAYHTDAVKKVIEKTSQG
                     LDQPVW"
     gene            complement(4632..>4851)
                     /locus_tag="VBC94_001843"
     CDS             complement(4632..>4851)
                     /locus_tag="VBC94_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6980087.1"
                     /translation="TAWDYGEYVEIDDFYVQESYRGQGIGTRLQFDASRGFKKVILIS
                     EEENRKMYEHQGYEEVAYYWTALRSPRK"
BASE COUNT         1575 a          862 c          750 g         1664 t
ORIGIN      
        1 gccacttggt tatcttttaa ttttggtaat tggtgaccta aacgacggaa atcattctga
       61 gtaattaggt acataaaacc cgtttttgtg atatcaacat gtttgacgtt tttagatgtg
      121 atattaagtt cacttgattg actaactggc atcgaaatca ttgtctgatt ataagtgata
      181 gcttccttag atgacttacc tagtttcttc aaaatctttt cttcaaatat atttttcgcg
      241 tcaccttttg aattatctat tgataaactt actgattttg gaaatagtcc ggtaacaaca
      301 ttttgtgtat ttgaatagag tgagactgtt gtagcaatag taactagagc cataacagct
      361 aataaagtga tacttgctaa ccctactgca ttttgcttca ttcgaaaaat catttgcgaa
      421 gttgatacaa aatgctcaga tttataataa taatgcttgt tttgacgcaa cctttttaag
      481 taccatgtca taaaactaat ataaaaaaga taagtaccag caataactaa aagtactgca
      541 aagaagaaac gatagataac agctaatgca ggtgctttac ctgaagtaag agccattgta
      601 taagcgatgg cgatagctac taacgctaaa attgcaagta taagattacc ttttggttct
      661 ttttctccct gttgtttctt tctaaaaaga cttaatgggg atgataaatg aacatgtcga
      721 ataactggaa cttctaaagt cagaaaaata cctgtaaata taacgataca aataataaat
      781 ggccataaac ttaaactaag atttagtgca tgatagttaa taatgttgac aaaaattaaa
      841 taaataaatt tagcaaaaaa agcactaaac agacttccta tagaaataag aaatatataa
      901 ataataaaca gctctagact agctacacgc gcaacttgac gtttattcat ccccaaaatg
      961 ttatacagtc caaattcact actacgctgt ttcatcaaga tattgtagct ataatgttcc
     1021 atgactaccg caaagattga caaaacaatc actccaagaa aaagaaccgt tgccgctgtc
     1081 cccatatctc ttccaacagc actcattaag attagtagcg tagaacaagt caatgaataa
     1141 agtaataaac tggctaagag gaatggtatg tactggtcta tagaatgttt taaattatgc
     1201 caggctattt tgagataaaa catattattc accccctaat atggcagaca ttgttaaaga
     1261 aatatcttta ttgaattcgc tattattctt attaccacga taaagttggt gaaaaatacg
     1321 tccatctttg ataaataaaa cgcgtttggc atgactagca gcatttgctg aatgggttac
     1381 catcaaaata gtttgtccat ccaagttaat agtttcaaat aaattcagca agtcttctga
     1441 attacggtaa tctaacgctg ctgttggttc atctgctaat aaaatttgag ggttggttat
     1501 caaactacga gcaatcgcta cacgttgctt ttgcccacca gagagctcga aaggtctctt
     1561 atctaataag tcatcaattc tcagatgaga agataattct gacaaacgat gatccatttc
     1621 tttgtaacgt ttacgatcaa gaactaaagg aagatagata ttatctctta cagagagagt
     1681 atccaaaagg ttgaagtcct gaaagacgaa accaagattt ttcaaacgaa aactcgctaa
     1741 ttttgcctct ttaattttcg taatatcttc cccatttaaa atcacttgtc catttgtcgg
     1801 tttttctaaa gtagctaaaa tatttagcag agttgttttt cccgaaccag actctcccat
     1861 aatagcaatg aattcccctt gttcaacttt aaaatcgacg tcttgtagag cccttgtctc
     1921 ttcttttgaa aaacgtgtac gaaaaacttt ttctaagtga ttgatttcta ataacatata
     1981 attctccttt atttattata ccctaatttt gattgattct actctttaac caagactgtg
     2041 actcttgtaa aggaaggctg gttacttgat ctggtaagat aggggtgttc tcgtatttat
     2101 atccttgtcg atcactaact attcctgtag ttaaagctaa tctagcaccg tcgtaaagca
     2161 tgaaagtttc attaacagtc gtatatcctg ctgtagcttg accaaagctt ttaacattag
     2221 gtaatccttt aaatgataaa aaagtcattt ctgccgaact agcagtttta tgattagtta
     2281 taatagcgat tggaacatgc ttagtattga ttgttttcct agatattttt aatgcttcta
     2341 gcggtatatt tttcatcgtg attgttttct tatttccata cttatcagta taatgaaata
     2401 gggtatcatt tggtaaaata gaggctaacc ccccaatcat agggatcata ttgccgccag
     2461 tattattcga taagtctaaa atgagtccct tataactttt ttccatcaat atattttgta
     2521 atttaccact ataatggcta atagatcgtt tatctaactt acttatagaa ggaatagtaa
     2581 ttaagagaat gtcttcgttt aatcttcgat attctggata tcgactatct agggtatctc
     2641 ttcgtacagt ctctttggat aatatttgac tatgtttacc gcctccatac cgtactgctt
     2701 cctgtacaat gttgcgactc tcttgataag attttgacgt tttcaacttt ttttctgatc
     2761 gttgtcttat aatttgccat tgacgacctt gcgaatacaa accacgctgt tcaacacgat
     2821 ctaaaataac acgtccataa cgttcgctac taggaggtaa tagataaata tttaatgctg
     2881 aaccataata atgaactacc actcctaaaa ttcctaaaac tataagcatt ggaactacaa
     2941 aaagtaaaac tatttttcgc cgcatctctt gctccccctt tctctttaag tctattatat
     3001 ggcaaaatgg tttactataa ccttacatct taagacctat ttcttacaaa tttgaaagtt
     3061 ttaaaagctt tataaactat aattttgata taatatctta cataacaaat ggaaagtacg
     3121 gtgaaaacat atggccaaga caagtaaaaa attgacaaaa tctttagggc ctattggtaa
     3181 attaatttca ataatccctg acacaactga acttattgga aaagcaattg ataattctcg
     3241 tccaattatt gaaaaagaac ttgaccgtcg tcatgaaaaa aaacagactt atactcagct
     3301 tgataatgta gtacaccttg atatcaatga tgcgcaagtt tacttagagc aacgccattt
     3361 taatgtatca aggattgttg ctgaaccaaa ttcaaagtgg gcaaccctac atcctaatca
     3421 agttgtgaat atgtcgccta aagctggtag ggtcagaatc ggttcattag tgaagttgta
     3481 ctatattaca gaggatatcc ttgaagaaag taagatacta cttttatcta aatccgaaaa
     3541 aagtaaagca cgacaacaac ggttaacaga agtattgagt acccctagtc aattactcaa
     3601 aaaaaatcga aataaataag catgatcgaa ttgaccatgc ttatttattt ttaccaaaca
     3661 ggttgatcta aaccttgaga tgttttttcg ataacttttt taaccgcatc tgtatggtaa
     3721 gctttcacaa cttctttaat ggctttagct tttgatttgt cttgccaacc tttttgagca
     3781 actaaaaggt tgtaccattg ttttgcattt ttactttttg gttcaataaa aatagctgat
     3841 ttaggattta ttcctgcttc tgtcacaaaa tcattattaa taactgctgc atctgtggaa
     3901 tccaaagaac gtgcggtctg tgctgcatct acttctttta aatctaaaga tttaggattg
     3961 gaaacaacat ctgacattgt tgctaatgta tcacctgata ctttcaattt tagcaagcct
     4021 gctgattgta acaagtagag agagcgactt tcgttaactg catcgtttgg gatagtaata
     4081 gttgctttat ttggaatttc agaaactgtt tggtatttac ctttaccgtt cttagtacca
     4141 gagtataatc taaaggaagt aaagtatgtc tcagcaacgg aaacaagatt ggtcttattt
     4201 gctttattcc aattgtttaa gtaattataa tgctggaaag cattaatatc aatctcatca
     4261 ctttctaaag ctttattagg ttgtgtataa tctgtgaatt ccgtaaattt tagcttaacg
     4321 ccttttttct taactaattc ttcgatttta tcccaacgag ctttttcagt tttactcttt
     4381 accattgttc caactctaac gactttggcg tcttcatttt tagactgacc acaggctcct
     4441 aaaataagtg ctgaaattac tactgttgtc gttaacccta atagcttttt gattttcata
     4501 atttatttct cctttgatat ggttgtatga ttaatcaact gatactagtg taacaaaaag
     4561 ctaatggatt aaaaaatact tattatcaat caagagatat agcttttaac tatatcattt
     4621 cttttataat tttactttcg agggctccgt aaagcggtcc aataataggc gacttcctca
     4681 taaccttggt gctcatacat ttttctgttt tcttcttctg aaattaagat aactttctta
     4741 aaccccctac ttgcatcaaa ttgtaggcgc gtaccaattc cttgtcctct gtaggactct
     4801 tggacataaa aatcatctat ttccacgtat tcaccatagt cccaagctgt t
//
LOCUS       DATROQ010000113         4818 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.114, whole genome shotgun sequence.
ACCESSION   DATROQ010000113 DATROQ010000000
VERSION     DATROQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.114"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(24..932)
                     /locus_tag="VBC94_001844"
     CDS             complement(24..932)
                     /locus_tag="VBC94_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6980088.1"
                     /translation="MKTIKDWQINICLATYNGQKYLRQQLDSIIQQGYTDWICLIRDD
                     GSTDDTVAIIKEYVNRDSRFIFINSNDDRKLGSHRSFYELVNYKKADFYVFSDQDDVW
                     KENRLERYLEEAEKFNQELPLLVYSNWTSVDEKLTVLKEHNPATVIQEQIAFNQINGM
                     VIMMNHELAKLWEYRQIGAHDSYVGTLAYAVGNVAYISDSTVLWRRQVGAESLNNYGR
                     QYGVATFWQMINTSFDRASLIFAQVSDKMSLERKLFFSRFIELKNANLIRRIYLLSKL
                     KLRRKSLKETVAMTILLLTGYGKPKA"
     gene            complement(1142..3124)
                     /locus_tag="VBC94_001845"
     CDS             complement(1142..3124)
                     /locus_tag="VBC94_001845"
                     /inference="COORDINATES: protein motif:HMM:NF018035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fn3-like domain-containing protein"
                     /protein_id="HEO6980089.1"
                     /translation="MSGTSMASPHVAGLMTMLQSHLAEKYKGMNLDSKKLLELSKNIL
                     MSSATALYSEEDKAFYSPRQQGAGVVDAEKAIQAQYYVTGNDGKVKINLKREGDKFDI
                     TVTIHKLVEGVKELYYQANVATEQVNKGKFALKPQALLDTNWQKVILRDKETQVRFTI
                     DASQFSQKLKEQMANGYFLEGFVRFKEAKDSNQELMSIPFVGFNGDFANLQALETPIY
                     KTLSKGSFYYKPNDTTHKDQLEYNESAPFESNNYTALLTQSASWGYVDYVKNGGELEL
                     APESPKRIILGTFENKVEDKTIHLLERDAANNPYFAISPNKDGNRDEITPQATFLRNV
                     KDISAQVLDQNGNVIWQSKVLPSYRKNFHNNPKQSDGHYRMDALQWSGLDKDGKVVAD
                     GFYTYRLRYTPVAEGANSQESDFKVQVSTKSPNLPSRAQFDETNRTLSLAMPKESSYV
                     PTYRLQLVLSHVVKDEEYGDETSYHYFHIDQEGKVTLPKTVKIGESEVAVDPKALTLV
                     VEDKAGNFATVKLSDLLNKAVVSEKENAIVISNSFKYFDNLKKEPMFISKEGKVVNKN
                     LEEITLVKPQTTVTTQSLSKEITKSGNEKVLTSTNNNSSRVAKIISPKHNGDSVNHTL
                     PSTSDRATNGLFVGTLALLSSLLLYLKPKKTKNNSK"
     gene            complement(3128..3445)
                     /locus_tag="VBC94_001846"
     CDS             complement(3128..3445)
                     /locus_tag="VBC94_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001870148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEO6980090.1"
                     /translation="MTKITHATNAGVVGIVIFNDQEKRGNFLIPYRELPVGVISKVDG
                     ERIKNTSSQLTFNQSFEVVDSQGGNRMLEQSSWGVTAEGAIKPDVTASGFEIYSSTYN
                     NQY"
     gene            complement(3472..>4818)
                     /locus_tag="VBC94_001847"
     CDS             complement(3472..>4818)
                     /locus_tag="VBC94_001847"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6980091.1"
                     /translation="AILKLTLITTSILLMHSNQVNAEEQELKNQEQSPVIANVAQQPS
                     PSVTTNIVEKTSVTAASASNTVKEMGDTSVKNDKTEDELLEELSKNLDTSNLGADLEE
                     EYPSKPETTNNKESNVVTNASTAIAQKVPSAYEEVKSESKSSLAVLDTSKITKLQATT
                     QRGKGNVVAIIDTGFDINHDIFRLDSPKDDKHSFKTKAEFEELKAKHNITYGKWVNDK
                     IVFAHNYANNTETVADIAAAMKDGYGSEAKNILHGTHVAGIFVGNSKRPAINSLLLEG
                     AAPNAQVLLMRIPDKIDSDKFGEAYAKAIIDAVNLGAKTINMSLGKTADSLIALNDKV
                     KLALKLASEKGVAVVVAAGNEGAFGMDYSKPLSTNPDYGTVNSPAISEDTLSVASYES
                     LKTISEVVETTIEGKLVKLPIVTSKPFDKGKAYDVVYANYGAKKILKVRTLKVRLH"
BASE COUNT         1438 a          944 c          727 g         1709 t
ORIGIN      
        1 ttgatttcct tcaaccaaaa aaattatgct tttggtttac cgtaacctgt taaaagtagg
       61 atagtcatag caactgtttc tttcaatgat ttccgacgaa gttttaattt tgataaaaga
      121 taaatccttc ttattagatt ggcatttttt aattctataa aacgactaaa gaagagttta
      181 cgctccaggg acattttatc ggatacttga gcaaaaataa gcgaagcacg atcaaaagaa
      241 gtatttatca tctgccaaaa cgtagccacc ccatattgtc tcccgtaatt atttagcgac
      301 tctgccccta cttgtctacg ccaaaggact gtactgtcag aaatataagc cacatttcca
      361 acggcgtaag ccagagttcc aacataagaa tcatgggcgc caatttgacg gtattcccaa
      421 agctttgcta actcatgatt catcatgata accataccat ttatttggtt aaaggcaatt
      481 tgttcttgaa taactgttgc tggattatgt tctttcaata ctgttaattt ttcatctaca
      541 gaagtccagt tcgaataaac aagcaatggt aattcttggt taaatttctc tgcttcttca
      601 aggtaacgtt ctaaacgatt ttctttccaa acatcatcct ggtctgagaa aacataaaaa
      661 tctgcttttt tatagttaac caactcgtaa aaactgcggt gacttcctaa ctttctatca
      721 tcgttagaat taataaatat aaaacgtgaa tctctattaa catattcttt aataatagct
      781 actgtatcat cagtagatcc atcgtccctt attaaacaaa tccagtcagt ataaccttgt
      841 tgtattatgc tatctaattg ctggcgaagg tatttttgac cattataggt cgctaagcaa
      901 atatttattt gccaatcttt tatcgttttc attgctatca aaactcctta aggctagttc
      961 atcttatgaa agcttacaaa gcgaacagta tcacatacta atcgtaatta taatattata
     1021 atattatatg ccgttaatca atataaaatt aatcaaaaat aagagtcaat accctaaaag
     1081 tccagcttaa cataatgtta aactgaactt ttcatcatat taccgagtag tttacttagt
     1141 tttatttact attattttta gtctttttgg gtttcaagta aagaagtaaa ctagataaca
     1201 atgccaaagt gccaacaaat agaccattcg ttgctctatc tgatgtacta ggtaaggtat
     1261 ggttaacaga atccccgtta tgtttaggtg atatgatctt agctactctg ctactattat
     1321 tgtttgtaga agtgaggact ttctcatttc ctgatttagt tatttcttta gacaatgatt
     1381 gagtagtaac tgtagtttga ggcttaacta atgttatttc ttctagattc ttgtttacta
     1441 cttttccttc tttagaaata aacataggtt ctttcttcaa gttatcaaaa tatttgaaac
     1501 tgttagaaat tactatagcg ttttctttct ctgatactac tgccttattc aagaggtcag
     1561 acaattttac cgttgcaaaa ttaccagctt tatcttccac aacaagtgtc aaggccttag
     1621 ggtctaccgc aacctcactc tctcctatct taaccgtttt aggaagtgtc actttacctt
     1681 cttgatctat atggaaataa tggtaagaag tctcatcccc atattcttca tcttttacaa
     1741 catgagataa aactaattgt aaacgatatg taggaacata actactttcc ttaggcatgg
     1801 ctaagcttaa tgttcgatta gtttcatcaa actgagctcg tgaaggaaga tttggtgact
     1861 tagtacttac ttgaacttta aagtctgact cctgactatt tgctccttct gctactggtg
     1921 tgtaacgtaa gcgataagta taaaaaccat ctgctacaac tttgccatcc ttatctaaac
     1981 cactccactg aagagcatcc atacgataat gaccatcact ttgctttgga ttattatgga
     2041 aatttttacg ataagatggt aaaaccttac tttgccaaat aacatttcca ttttgatcta
     2101 gaacttgagc agaaatatcc ttaacatttc ttaagaaagt tgcctgggga gtgatttcgt
     2161 ccctatttcc atctttattt ggagaaatgg caaaatatgg attattcgct gcatctcttt
     2221 ccaaaagatg aattgtttta tcctcaacct tattctcaaa agttcctaaa ataattcttt
     2281 ttggactctc cggtgctaat tctaactccc caccattttt gacataatca acatagcccc
     2341 aagacgctga ttgtgttaac aaggcagtat agttgttgct ttcaaaagga gctgattcat
     2401 tgtactccaa ttggtcttta tgagttgtat catttggttt atagtagaaa ctacctttag
     2461 aaagcgtctt ataaatcggt gtttcaagtg cttgtaagtt cgcaaaatca ccattaaatc
     2521 ctacaaaagg aatactcatt aactcctgat tactatcctt ggcttcttta aaacgtacaa
     2581 aaccttctaa gaaataacca tttgccatct gttcttttaa tttctgacta aattgactag
     2641 catcaatagt aaatcgaact tgtgtttctt tatcacgaag aattactttc tgccaattag
     2701 tatctagcaa ggcttgtggt ttaagggcaa atttaccttt atttacttgt tctgttgcta
     2761 cattagcttg ataatacaat tctttgacac cttctacaag tttatgaatt gtaactgtga
     2821 tatcaaattt atctccctct cgtttgagat taattttaac tttgccatcg tttccagtaa
     2881 cataatattg agcttggata gctttttcag catcaactac acctgcacct tgctgacgtg
     2941 gtgaataaaa cgccttatcc tcttcactat ataatgctgt tgctgagctc atgaggatgt
     3001 ttttagacaa ttctagcaat tttttagaat ctaaattcat ccctttatat ttctcagcca
     3061 aatgactttg aagcattgtc attaatcctg caacatgtgg tgaagccata cttgtaccag
     3121 acattgttta gtattgatta ttataggttg aagaataaat ttcaaagcca gaagctgtta
     3181 catcaggctt gattgctcct tcagctgtca cgccccaact tgattgttcc agcatacgat
     3241 tgccaccttg gctatcaact acttcaaaac tctggttaaa tgttaactga cttgaagtat
     3301 tttttatacg ctcgccatct actttactaa taacccccac aggtaattca cggtaaggaa
     3361 ttagaaaatt tccacgtttt tcttgatcgt taaaaataac gataccaaca acacctgcat
     3421 ttgtagcatg agtgatttta gtcataaaat caagtccacc accacgctca attaatgcaa
     3481 tcttaccttt aaagtcctta ccttcaaaat cttttttgca ccataattgg cataaaccac
     3541 atcgtaggcc ttacctttgt caaaaggttt agaagtcaca atcggcaact taactaactt
     3601 accttcaata gttgtttcaa cgacctcact gatagtttta agtgattcat agctagcaac
     3661 actcaaagta tcttcagaaa tagctggact attaaccgta ccgtagtcag gattagttga
     3721 taatggtttg ctataatcca taccaaatgc accttcattt ccggcagcca caacaactgc
     3781 aacgcccttc tcagaagcta atttaagtgc taatttaact ttatcattga gagcaattaa
     3841 agaatcagcc gtttttccca ggctcatatt aatcgttttt gctcctagat taacagcgtc
     3901 tatgattgct ttagcatatg cttctccaaa tttgtccgaa tcaattttat ctggaatacg
     3961 cattaataag acttgagcat ttggcgctgc accttctaaa agaagactat tgattgctgg
     4021 acgtttacta ttacctacaa aaataccagc aacgtgtgta ccatgcaaaa tattctttgc
     4081 ttctgaccca taaccatctt tcatagctgc tgcaatatca gccaccgttt ctgtattgtt
     4141 ggcgtagtta tgtgcaaaaa caatcttatc gttaacccat ttcccataag tgatattatg
     4201 ttttgctttt aattcctcaa attctgcctt agttttaaag ctgtgcttat catcttttgg
     4261 gctatctaaa cgaaaaatat catggttaat atcaaagcca gtatcaataa tagctactac
     4321 atttcccttt cctctttggg ttgtggcttg taattttgtt attttagatg tatcaagaac
     4381 agcaagcgat gacttgcttt ctgacttcac ctcttcatat gctgagggaa ctttctgtgc
     4441 tattgcagtt gaagcatttg ttactacatt gctttcttta ttgttggttg tctctggttt
     4501 agagggatat tcttcttcaa gatcagcccc caaattagac gtatcaaggt ttttagataa
     4561 ctcttctaat aattcatctt ctgttttgtc attttttaca gatgtatcac ccatttcttt
     4621 cactgtatta ctagcagaag cagctgttac agatgttttt tcaacaatat tagtagttac
     4681 cgatggcgat ggctgttgag caacattagc aattacaggt gattgctctt ggttttttaa
     4741 ttcttgctcc tctgcattca cttgattgct atgcattaat aaaatactag ttgttataag
     4801 tgttaacttt aaaatagc
//
LOCUS       DATROQ010000114         4775 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.115, whole genome shotgun sequence.
ACCESSION   DATROQ010000114 DATROQ010000000
VERSION     DATROQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.115"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..1422
                     /locus_tag="VBC94_001848"
     rRNA            <1..1422
                     /locus_tag="VBC94_001848"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1477..1549
                     /locus_tag="VBC94_001849"
     tRNA            1477..1549
                     /locus_tag="VBC94_001849"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1510..1512,aa:Ala,seq:tgc)
     gene            1704..4606
                     /locus_tag="VBC94_001850"
     rRNA            1704..4606
                     /locus_tag="VBC94_001850"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4681..>4775
                     /gene="rrf"
                     /locus_tag="VBC94_001851"
     rRNA            4681..>4775
                     /gene="rrf"
                     /locus_tag="VBC94_001851"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1338 a          976 c         1383 g         1078 t
ORIGIN      
        1 aacctgcctc atagcggggg ataactattg gaaacgatag ctaataccgc ataagagtaa
       61 ttaacacatg ttagttattt aaaaggagca attgcttcac tgtgagatgg acctgcgttg
      121 tattagctag ttggtgaggt aaaggctcac caaggcgacg atacatagcc gacctgagag
      181 ggtgatcggc cacactggga ctgagacacg gcccagactc ctacgggagg cagcagtagg
      241 gaatcttcgg caatggacgg aagtctgacc gagcaacgcc gcgtgagtga agaaggtttt
      301 cggatcgtaa agctctgttg ttagagaaga acgttggtag gagtggaaaa tctaccaagt
      361 gacggtaact aaccagaaag ggacggctaa ctacgtgcca gcagccgcgg taatacgtag
      421 gtcccgagcg ttgtccggat ttattgggcg taaagcgagc gcaggcggtt ctttaagtct
      481 gaagttaaag gcagtggctt aaccattgta cgctttggaa actggaggac ttgagtgcag
      541 aaggggagag tggaattcca tgtgtagcgg tgaaatgcgt agatatatgg aggaacaccg
      601 gtggcgaaag cggctctctg gtctgtaact gacgctgagg ctcgaaagcg tggggagcaa
      661 acaggattag ataccctggt agtccacgcc gtaaacgatg agtgctaggt gttaggccct
      721 ttccggggct tagtgccgca gctaacgcat taagcactcc gcctggggag tacgaccgca
      781 aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat
      841 tcgaagcaac gcgaagaacc ttaccaggtc ttgacatcct tctgaccggc ctagagatag
      901 gctttctctt cggagcagaa gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga
      961 gatgttgggt taagtcccgc aacgagcgca acccctattg ttagttgcca tcattaagtt
     1021 gggcactcta gcgagactgc cggtaataaa ccggaggaag gtggggatga cgtcaaatca
     1081 tcatgcccct tatgacctgg gctacacacg tgctacaatg gttggtacaa cgagtcgcaa
     1141 gccggtgacg gcaagctaat ctcttaaagc caatctcagt tcggattgta ggctgcaact
     1201 cgcctacatg aagtcggaat cgctagtaat cgcggatcag cacgccgcgg tgaatacgtt
     1261 cccgggcctt gtacacaccg cccgtcacac cacgagagtt tgtaacaccc gaagtcggtg
     1321 aggtaacctt ttaggagcca gccgcctaag gtgggataga tgattggggt gaagtcgtaa
     1381 caaggtagcc gtatcggaag gtgcggctgg atcacctcct ttctaaggat aaggaaacct
     1441 gccatttgcg tcttgtttag ttttgagagg tcttgtgggg ccttagctca gctgggagag
     1501 cgcctgcttt gcacgcagga ggtcagcggt tcgatcccgc taggctccat tgaatcgaaa
     1561 ggttcaaatt gttcattgaa aattgaatat ctatatcaaa ttccacgatc tagaaataga
     1621 ttgtagaaag taacaagaaa ataaaccgaa aacgctgtga atatttaatg agttttctag
     1681 ttttaaagaa actagtttaa taaggttaag ttaataaggg cgcacggtgg atgccttggc
     1741 actagaagcc gaagaaggac gtgactaacg acgaaatgct ttggggagct gtaagtaagc
     1801 gctgatccag agatgtccga atgggggaac ccactagtta atgactagta tccatatctg
     1861 ttaaggatat gagaaggaag acgcagtgaa ctgaaacatc taagtagctg caggaagaga
     1921 aagcaaaagc gattgcctta gtagcggcga gcgaaacggc aggagggcaa accgaagtgt
     1981 ttacacttcg gggttgtagg actgcgatat aggattaatc attatagaag aattacctgg
     2041 gaaggtaagc caaagagagt aacagcctcg tatttaaaat gatagattaa ccttagcagt
     2101 atcctgagta cggcgagaca cgcgaaatct cgtcggaatc tgggaggacc atctcccaac
     2161 cctaaatact ctctagtgac cgatagtgaa ccagtaccgt gagggaaagg tgaaaagcac
     2221 cccgggaggg gagtgaaata gaacctgaaa ccgtgtgcct acaacaagtt cgagcccgtt
     2281 aatgggtgag agcgtgcctt ttgtagaatg aaccggcgag ttacgatatg atgcgaggtt
     2341 aagttgaaga gacggagccg tagggaaacc gagtcttaat agggcgccat agtatcatgt
     2401 tgtagacccg aaaccatgtg acctacccat gagcaggttg aaggtgaggt aaaactcact
     2461 ggaggaccga accagggcac gttgaaaagt gcttggatga cttgtgggta gcggagaaat
     2521 tccaaacgaa cttggagata gctggttctc tccgaaatag ctttagggct agcgtcgatg
     2581 ttaagtctct tggaggtaga gcactgtttg ggtgaggggt ccatcccgga ttaccaatct
     2641 cagataaact ccgaatgcca acgagatata atcggcagtc agactgcgag tgctaagatc
     2701 cgtagtcgaa agggaaacag cccagaccac cagctaaggt cccaaaatat atgttaagtg
     2761 gaaaaggatg tggggttgca cagacaacta ggatgttagc ttagaagcag ctattcattc
     2821 aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta ccggggctaa
     2881 aacatattac cgaagctgtg gatgacacaa aagtgtcatg gtaggagagc gttctatgtg
     2941 tgaagaaggt gtaccgtgag gagcgctgga acgcatagaa gtgagaatgc cggtatgagt
     3001 agcgcaagac aggtgagaat cctgtccacc gtaagactaa ggtttccagg ggaaggctcg
     3061 tccgccctgg gttagtcggg acctaaggag agaccgaaag gtgtatccga tggacaacag
     3121 gttgatattc ctgtactaga gtatatagtg atggagggac gcagtaggct aactaaacca
     3181 gacgattgga agtgtctggt caaacagtga ggtgtgatat gagtcaaatg cttatatctt
     3241 taacattgag ctgtgacgag gagcgaagtt tagtagcgaa gttagtgatg tcacactgcc
     3301 aagaaaagct tctagcgata catatactct acccgtaccg caaaccgaca caggtagtcg
     3361 aggcgagtag cctcaggtga gcgagagaac tctcgttaag gaactcggca aaatggcccc
     3421 gtaacttcgg gagaaggggc gctgatttta gatcagccgc agtgaatagg cccaagcaac
     3481 tgtttatcaa aaacacagct ctctgctaaa tcgtaagatg atgtataggg ggtgacgcct
     3541 gcccggtgct ggaaggttaa gaggagtgct tagcgtaagc gaaggtatga attgaagccc
     3601 cagtaaacgg cggccgtaac tataacggtc ctaaggtagc gaaattcctt gtcgggtaag
     3661 ttccgacccg cacgaaaggc gtaatgattt gggcactgtc tcaacgagag actcggtgaa
     3721 attttagtac ctgtgaagat gcaggttacc cgcgacagga cggaaagacc ccatggagct
     3781 ttactgcagt ttgatattga gtatctgtac cacatgtaca ggataggtag gagcctatga
     3841 taacgggacg ccagtttcgt ttgaggcgtt gttgggatac tacccttgtg ttatggctac
     3901 tctaacccag ataggcaatc cctatcggag acagtgtctg acgggcagtt tgactggggc
     3961 ggtcgcctcc taaaaggtaa cggaggcgcc caaaggttcc ctcagactgg ttggaaatca
     4021 gtcgtagagt gtaaaggtat aagggagctt gactgcgaga gctacaactc gagcagggac
     4081 gaaagtcggg cttagtgatc cggtggttcc gtatggaagg gccatcgctc aacggataaa
     4141 agctaccctg gggataacag gcttatctcc cccaagagtt cacatcgacg gggaggtttg
     4201 gcacctcgat gtcggctcgt cgcatcctgg ggctgtagtc ggtcccaagg gttgggctgt
     4261 tcgcccatta aagcggcacg cgagctgggt tcagaacgtc gtgagacagt tcggtcccta
     4321 tccgtcgcgg gcgtaggaaa tttgagagga tctgctccta gtacgagagg accagagtgg
     4381 acttaccgct ggtgtaccag ttgtcttgcc aaaggcatcg ctgggtagct atgtagggaa
     4441 gggataagcg ctgaaagcat ctaagtgcga agcccacctc aagatgagat ttcccatgat
     4501 tttatatcag taagagccct gagagatgat caggtagata ggttaggagt gtaattgtgg
     4561 tgacacattt agcggactaa tactaatagc tcgaggactt atccaaaaag agatattgac
     4621 aacgttacgg tttcttgtta gactatagat attcaatttt gagtgaataa gactcaatag
     4681 ttaagtgata attgcctagg agatacacct gttctcatgc cgaacacaga agttaagccc
     4741 tagaacgccg gaagtagttg ggggttgccc cctgt
//
LOCUS       DATROQ010000115         4683 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.116, whole genome shotgun sequence.
ACCESSION   DATROQ010000115 DATROQ010000000
VERSION     DATROQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.116"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(215..2191)
                     /gene="ftsH"
                     /locus_tag="VBC94_001852"
     CDS             complement(215..2191)
                     /gene="ftsH"
                     /locus_tag="VBC94_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEO6980092.1"
                     /translation="MKNNKNNGFLKNSFIYILLIIAVITTFQYYLKGTSSQNQQISYT
                     KLVKQLKAGEIKSISYQPSGGVVEVSGTYKKAKTIKSANSFTFLGGSVATKVTGFNSV
                     ILPNDSSIKSLVSAAEENNTNIQVKHESSSGTWISYIASFLPLVIMIGFFMMMMNQGG
                     GGGARGAMSFGKNKARSSSKDEVKVRFSDVAGAEEEKQELIEVVDFLKDPKRYKSLGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDA
                     KKAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNESIIVIAATN
                     RSDVLDPALLRPGRFDRKVLVGQPDVKGREAILRVHAKNKPLADNVDLKVVAQQTPGF
                     VGADLENVLNEAALVAARRNKKVIDASDIDEAEDRVIAGPSKKDRTISERERAMVAYH
                     EAGHTIVGLILSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKDDMKEQLAGLMGG
                     RVAEEIIFNAQTTGASNDFEQATAMARAMVTEYGMSEKLGPVQYEGNHAMMAGQMSPE
                     KSYSAQTAQLIDDEVRHLLNEARNKAADIINENRDTHKLIAEALLKYETLDAAQIKSI
                     FETGKMPETENDEDKARALSYDEIKEKMQEEDKK"
     gene            complement(2214..2756)
                     /gene="hpt"
                     /locus_tag="VBC94_001853"
     CDS             complement(2214..2756)
                     /gene="hpt"
                     /locus_tag="VBC94_001853"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEO6980093.1"
                     /translation="MLENDIKKVLYSEEDIILKTKELGAKLTADYAGKNPLLVGVLKG
                     SVPFMAELLKHIDTHVEIDFMVVSSYHGGTTSSGEVKILKDVDTNIEGRDVIFIEDII
                     DTGRTLKYLRDMFKYRQANSVKVATLFDKPEGRLVDIDADYVCYDIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEIYSK"
     gene            complement(2761..4035)
                     /gene="tilS"
                     /locus_tag="VBC94_001854"
     CDS             complement(2761..4035)
                     /gene="tilS"
                     /locus_tag="VBC94_001854"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEO6980094.1"
                     /translation="MYNTILKDTLSKGLFTAHQKVLIAVSGGIDSINLLQFLYQYQKE
                     LSISIGIAHINHGQRKESEKEEEYIRQWGQIHDVPVFISYFQGIFSEDRARNHRYNFF
                     SKVMREEGYTALVTAHHADDQAETVFMRILRGSRLRYLSGIKQVSAFANGQLIRPFLP
                     YKKELLPNIFHFEDASNASSDYLRNRIRNIYFPTLERENNQLKDSLITLSEETECLFT
                     ALTDLTRSIEVTNCYDFLRQTHSVQEFLLQDYISKFPDLQVSKEQFRVILKLIRTKAN
                     IDYTIKSGYFLHKDYESFHITKIHPKTDSFKVEKRLELHNIQIFSQYLFSYGKFISQA
                     DITIPIYDTSPIILRRRKEGDRIFLGNHTKKIRRLFIDEKITLKDREEAVIGEQNKEL
                     IFVIVAGRTYLRKPSEHDIMKGKLYIENLEKR"
     gene            complement(4037..>4683)
                     /locus_tag="VBC94_001855"
     CDS             complement(4037..>4683)
                     /locus_tag="VBC94_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6980095.1"
                     /translation="TQTVAGINIDRSMYSASIAKLATLYASQEQVKLGKLSLDSKFEY
                     KDNVNQFPNSYDPSGSGKLEKKADHKLYTVKELLEATAKESDNVATNMLGYYVNNQYD
                     SMFQTQVDTISGMHWDMKKRQISPQAAGKMMEAIYYQNGDIVNYLSKTDFDNTRIPKN
                     IPVKVAHKIGDAYDYKHDAAIVYAEQPFIMIIFTDKSSYDDITKIADDVYQVLK"
BASE COUNT         1426 a          907 c          684 g         1666 t
ORIGIN      
        1 attttttctt ttatttttaa aaagttaata ttatttatag taactaacct tctatacttg
       61 tttaatggat agcattttta ccgttgtcat gttcatattt catcttctta attcacaaat
      121 ttaaacttca tcttcataaa aaacacccct caaattttat ctaaatttga agggtgtttg
      181 aaatttataa agttctttaa aaatatatga tgacttattt tttatcttct tcttgcattt
      241 tttctttgat ttcatcgtat gataacgctc tcgctttatc ttcatcattt tctgtctcag
      301 gcattttacc tgtctcgaaa atcgatttaa tctgagcagc atcaagagtc tcatatttta
      361 agagggcttc tgcaattaat ttatgagtat cacggttttc gttgataata tcagctgcct
      421 tattacgtgc ttcattaaga aggtgacgaa cttcatcatc aataagttgt gcagtttgag
      481 cagaatatga tttttcaggt gacatttggc cagccatcat tgcatggtta ccttcgtatt
      541 gaactgggcc aagtttctca ctcataccat attcagttac catagcgcgg gccatggcag
      601 tggcttgttc aaagtcattt gaggcacctg ttgtctgagc gttgaaaata atttcttccg
      661 ctacacgtcc tcccataaga cctgctaatt gctctttcat atcatctttt gaaagaagca
      721 tttgatcttc tttaggtaaa gcaatcatat atccacctgc acgaccacgt ggtacgatag
      781 taactttatg aacaactcgc gcatttgaaa gaatcaaacc gacaattgtg tgcccagctt
      841 catggtaagc aaccatagct ctttctcttt cagaaatagt acgatctttt ttagaaggac
      901 cagcaattac acggtcttct gcttcatcaa tatctgaagc atcaataact tttttatttc
      961 gtcgcgcagc aactaaagca gcttcattga gaacattctc caaatcagca ccaacaaatc
     1021 ctggggtttg ttgagctact acttttaagt caacattatc tgctaatggt ttatttttag
     1081 catgtactct cagaattgct tcacgacctt ttacatcagg ttgtccaact agaacttttc
     1141 tatcaaaacg tcctggacgc agtaatgcgg gatctaacac gtcactacgg ttggtagcag
     1201 cgataacgat aatactttcg tttccttcaa aaccatccat ttcaattaaa agttggttga
     1261 gcgtttgttc acgttcatcg tttccgccac ccataccagc tccacgtcga cgtccaacag
     1321 catcaatttc atcaatgaaa ataatagcac gttcagcttt tttagcatct tcaaacaaag
     1381 agcgtactcg actagctcca acaccgacaa acatttcaac aaaatctgaa ccggaaatac
     1441 taaagaatgg aacccctgct tcaccggcaa cagcttttgc taatagggtt ttacctgtcc
     1501 ctggaggtcc ttctaataga acccctgctg gaattcgggc acctagagat ttatatcgtt
     1561 taggatcttt aaggaagtca acgacttcta ttaattcttg cttttcttcc tctgctccag
     1621 caacatccga aaatcgaact ttaacctcat ctttactaga tgatcttgct ttatttttac
     1681 caaagctcat ggcaccacga gcgccaccgc caccgccttg gttcatcatc atcataaaga
     1741 aaccaatcat gataactaaa ggtaaaaaac tggcaatata agaaatccat gtcccacttg
     1801 aactttcatg tttgacctga atattagtgt tattttcttc agcagcgcta acaagagatt
     1861 ttattgaaga gtcatttggt agtataacag aattaaatcc tgtaacttta gtagcaacac
     1921 taccacctaa aaaagtaaaa ctattagctg atttaattgt tttggctttc ttatatgtcc
     1981 cactaacttc aacaacacca ccacttggct ggtatgaaat agacttaatt tctccagctt
     2041 ttaattgttt aacaagtttt gtataagaaa tttgctggtt ttgagagcta gttcctttta
     2101 aatagtattg aaaggttgta ataaccgcaa taatcaataa tatgtaaata aaggaatttt
     2161 tcagaaaacc attattttta ttatttttca tagtttaaga gagtaccttt tttctatttt
     2221 gagtaaattt cttcttttaa cacaccgaca taaggtaggt tacgatagtt ttcagcatag
     2281 tctaaaccga atcccacaat aaattcattt gggatatcgt aacaaacgta atcagcatca
     2341 atatctacca atcgcccttc tggcttatca aaaagggttg caactttgac agagttagct
     2401 tgacgatact taaacatatc tcgaaggtat tttaatgtac gtccagtatc aatgatgtct
     2461 tcaataaaga ttacatctct tccttcaatg ttagtatcaa catcttttaa aattttaact
     2521 tctccactac tagttgtacc accatgatag cttgaaacaa ccataaaatc aatttcaaca
     2581 tgtgtatcaa tatgctttaa tagttcagcc ataaaaggca ctgacccctt taatacacct
     2641 actaataatg gattcttacc tgcataatct gcagttaatt tagctcctaa ttctttcgtt
     2701 ttcagaataa tatcttcttc tgaatataaa acttttttta tatcattttc aagcatgatg
     2761 tcaccttttt tctaaatttt caatataaag tttgcccttc attatatcat gttcagaagg
     2821 ttttctcaaa tatgttctac ctgcaacaat cacaaatatc aactctttat tttgctcccc
     2881 aataacagct tcttctctgt cttttagagt tattttctca tcaataaata gacggcgaat
     2941 ttttttagtg tgatttccta aaaatatcct atccccttct tttcttcttc ttaaaataat
     3001 cggagaagta tcataaatag gaatagtaat gtctgcttga gaaatgaatt ttccatagga
     3061 aaataaatat tgtgagaata tttgtatatt atgcaattct aatctttttt caaccttaaa
     3121 actatccgtc ttaggatgga tttttgtaat atgaaaactc tcgtaatcct tatgaagaaa
     3181 atagcccgat ttaatagtat agtcaatatt ggctttagtt ctaatcaatt ttaatattac
     3241 tctgaactgt tctttactca cttgtaaatc tggaaatttg ctaatgtaat cttgcaataa
     3301 aaattcttgg acggaatgag tttgccttaa aaaatcatag cagtttgtta cttcgatgct
     3361 ccttgttagg tctgttaaag ccgtgaataa acactctgtt tcctcagaga gagttatgag
     3421 agaatctttc aattgattat tttctctttc caaagtagga aagtagatgt ttcttattcg
     3481 atttctcaaa taatcagacg aagcattgct agcatcttca aaatgaaaaa tatttggtaa
     3541 taactccttt ttatatggaa gaaatggtct aatcaattga ccatttgcaa aagcactcac
     3601 ttgcttaatc cctgataaat agcgtagcct gcttcctctt aaaattctca taaatacagt
     3661 ttcagcttga tcatcagcat ggtgagctgt tactaaagcc gtgtaacctt cttcacgcat
     3721 aacttttgaa aaaaaattgt agcgatggtt acgtgctcta tcttctgaaa atattccttg
     3781 aaaataagag ataaagacag ggacatcatg tatctgtccc cactgtctga tatattcttc
     3841 ttctttttct gattcttttc gctgtccatg atttatgtgt gcaataccaa tactaattga
     3901 taactctttt tgatactggt ataaaaattg caacaaattt attgaatcaa tgccaccaga
     3961 aacagcaatt agaactttct gatgagcggt aaataaccct ttggataagg tatctttcaa
     4021 aatagtgtta tacatattac tttaaaacct ggtaaacgtc gtcagcaatc ttagtgatat
     4081 catcgtaaga tgatttatct gtaaaaataa tcataataaa aggttgttct gcataaacaa
     4141 tagccgcatc atgtttgtag tcataagcat ctccaatttt atgtgctact ttaactggta
     4201 tatttttagg gattcttgta ttatcaaaat cagtctttga taggtaatta acaatatctc
     4261 cattttgata ataaattgct tccatcattt tacccgcagc ttgtggagaa atctggcgtt
     4321 tcttcatatc ccagtgcata ccagaaattg tgtctacttg tgtttgaaac attgaatcgt
     4381 attgattatt gacataataa cctagcatat ttgttgcaac attatctgat tccttggcag
     4441 ttgcttcaag taactcctta actgtatata atttatgatc agcttttttc tctaatttcc
     4501 cactaccact tggatcatag ctattaggaa attgattaac attatctttg tattcaaatt
     4561 tgctgtccaa tgacagtttt cctaatttta cttgctcttg gctagcataa agtgttgcta
     4621 attttgcaat acttgcagag tacattgacc tatcaatatt aataccagct actgtctgtg
     4681 tat
//
LOCUS       DATROQ010000116         4636 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.117, whole genome shotgun sequence.
ACCESSION   DATROQ010000116 DATROQ010000000
VERSION     DATROQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.117"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            62..135
                     /locus_tag="VBC94_001856"
     tRNA            62..135
                     /locus_tag="VBC94_001856"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:96..98,aa:Arg,seq:tct)
     gene            complement(208..687)
                     /gene="rlmH"
                     /locus_tag="VBC94_001857"
     CDS             complement(208..687)
                     /gene="rlmH"
                     /locus_tag="VBC94_001857"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEO6980096.1"
                     /translation="MKLKIITVGKLKEKYLKEGVAEYQKRLNRFSKIETIELADEKTP
                     DKASISENQRILDIEGERILSKIGERDYVIGLAIEGKQLPSESFSHLIDQKMISGYST
                     ITFVIGGSLGLSQKVKKRADYLMSFGLLTLPHQLMKLVLMEQIYRAFMIRQGTPYHK"
     gene            888..2117
                     /locus_tag="VBC94_001858"
     CDS             888..2117
                     /locus_tag="VBC94_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEO6980097.1"
                     /translation="MKKKLVSSLLKCSLIIIVSFAGGAFASFVMNHNDNIPNGGVTKT
                     SKVNYNNITPTTKAVKKVQNSVVSVINYKQQESRSDLSDFYSHFFGNQGGNTDKGLQV
                     YGEGSGVIYKKDGKNAYVVTNNHVIDGAKQIEIQLADGSKAVGKLVGSDTYSDLAVVK
                     IPSDKVSNIAEFADSSKLNIGETAIAIGSPLGTEYANSVTQGIVSSLKRTVTMTNEEG
                     QTVSTNAIQTDAAINPGNSGGALINIEGQVIGINSSKISSTSNQTSGQSSGNSVEGMG
                     FAIPSNDVVKIINQLESNGQVERPALGISMAGLSNLPSDVISKLKIPSNVTNGIVVAS
                     IQSGMPAQGKLKKYDVITKVDDKEVASPSDLQSLLYGHQVGDSITVTFYRGENKQTVT
                     IKLTKTSKDLAKQRANN"
     gene            2215..2988
                     /locus_tag="VBC94_001859"
     CDS             2215..2988
                     /locus_tag="VBC94_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEO6980098.1"
                     /translation="MEYLETININHIAPNPYQPRLEFNTKELEELADSIKINGLIQPI
                     IVRPSAVFGYELVAGERRLRAAKLAKLESIPAIIKSYNNDDSMQLAIVENLQRSNLSP
                     IEEAKAYSQLLQKKSMTHEELAKYMGKSRPYISNTIRLLNLPPLITSAIEEGKLSSGH
                     ARALLSLPDASQQKDWYQRILTEDISVRRLEKLLKQEKKTNHKSLQNKDVFLKHQENE
                     LAQFLGSKVKLTINKDGAGNIKIAFNNQEELNRIINTLK"
     gene            3197..4558
                     /gene="dnaA"
                     /locus_tag="VBC94_001860"
     CDS             3197..4558
                     /gene="dnaA"
                     /locus_tag="VBC94_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEO6980099.1"
                     /translation="MTENEQLFWNRVLELSRSQIAPAAYEFFVLEARLLKIEHQTAVI
                     TLDNIEMKKLFWEQNLGPVILTAGFEIFNAEITANYVSNDLHLQETSFSNYQQSSNEV
                     NTLPIRKIDSNLKEKYTFANFVQGDENRWAVSASIAVADSPGTTYNPLFIWGGPGLGK
                     THLLNAIGNQVLRDNPNARVLYITAENFINEFVSHIRLDSMEELKEKFRNLDLLLIDD
                     IQSLAKKTLGGTQEEFFNTFNALHTNDKQIVLTSDRNPNQLNDLEERLVTRFSWGLPV
                     NITPPDFETRVAILTNKIQEYPYDFPQDTIEYLAGEFDSNVRELEGALKNISLVADFK
                     HAKTITVDIAAEAIRARKNDGPIVTVIPIEEIQIQVGKFYGVTVKEIKATKRTQDIVL
                     ARQVAMYLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMVAQDDNLRIEIETIKN
                     KIR"
BASE COUNT         1636 a          793 c          788 g         1419 t
ORIGIN      
        1 aaatagtaca tatgaaaact tgacagcctg cctaatattt tggtagaata acagagttga
       61 tggttccata gctcagctgg atagagcatt cgccttctaa gcgaacggtc gcaggttcga
      121 atcctgctgg aatcagataa tgaaagccta gatagattat ctaggctttt ttatttgttt
      181 ttagcggttg tttcataaat ggaacgatta tttgtggtaa ggagtgcctt gacgaatcat
      241 aaaagcacga taaatttgtt ccatcaagac taatttcata agttgatgag gtaaggttaa
      301 cagtccaaaa ctcattaaat aatcagcacg ttttttcact ttttgagata aacccaaact
      361 accaccaata acaaaagtaa tagtagagta gccggatatc attttttgat cgattagatg
      421 gctaaatgat tcagatggca attgtttccc ttctatagcc aaaccaatga cataatccct
      481 ctcacctatt ttactcaaaa tacgttcccc ttctatatct aaaattcttt gattttcaga
      541 tatactagct ttatcaggtg tcttttcatc agctagctca attgtctcaa ttttagaaaa
      601 gcgattcaga cgtttctgat actctgcaac accttcttta aggtatttct ctttcaattt
      661 tccaactgta attattttta atttcatagc tacattataa cataatccac aatattttca
      721 caatttatcc acatgtttat ccttacatat ccccttattt tataggcaat ctataactat
      781 aatcaaggtt atccacaact tgtggatatt tccttttttt agcttttaag gtataattaa
      841 gaagcttgtc ataagattag tataaaaatt tacggaggta gctatttgtg aaaaaaaaat
      901 tagtctcatc acttctaaag tgttctctaa tcattattgt tagctttgct ggtggagcat
      961 ttgctagttt tgtcatgaat cataatgaca atattccaaa tggtggtgtc actaaaacta
     1021 gtaaagtaaa ttataataac ataacgccta caacaaaagc tgttaaaaag gtacaaaata
     1081 gtgttgtttc tgttatcaat tataaacaac aagagagtcg ttctgaccta tcagacttct
     1141 atagtcattt ttttggtaat caggggggca acactgataa gggcttacaa gtttacggtg
     1201 aaggctctgg agtcatctat aaaaaagatg gtaaaaatgc ctatgttgtc actaataacc
     1261 acgtcattga tggggctaaa caaattgaaa ttcaactagc tgatggctca aaagcagttg
     1321 ggaaacttgt tgggtcagat acctactctg atttagccgt cgtcaaaatt ccatcagata
     1381 aagtttcaaa tattgcagaa tttgctgatt catcaaaact caacattggt gaaactgcta
     1441 tagcgatcgg aagccctctt ggaactgagt atgcaaattc tgtaactcaa ggtattgtat
     1501 ctagtttaaa aagaactgta acaatgacta atgaagaagg acaaacagtt tctacaaatg
     1561 ctatccagac ggatgctgct atcaatcctg gtaattcagg tggagcactt atcaatattg
     1621 aaggacaggt tattggaatt aattctagta aaatttcttc tacatcaaat caaacctcag
     1681 gacaatcgtc aggaaatagc gttgaaggta tgggatttgc cattccttca aatgatgttg
     1741 ttaagattat caatcaactt gagagtaacg gacaagtaga gagacctgct ctaggtattt
     1801 ctatggctgg attaagtaat ttaccatccg atgttattag taaactgaaa atcccaagta
     1861 atgttactaa tggtattgta gtagcatcta tccaatctgg catgccagct caaggcaaac
     1921 taaagaaata cgatgtcatt actaaagttg acgataaaga agtagcatct ccaagtgatt
     1981 tacaaagttt actctatggc caccaggtag gggattccat aacagtaacc ttttatcgtg
     2041 gtgaaaataa acaaacagtc actataaaac ttactaaaac tagtaaagat ttagctaaac
     2101 aacgagcaaa taactaaatc aattctagtt attttaacta agatatagta aaattaaagg
     2161 ggttggtcag atagaccaat ccctttagta agtctataga atctgaggtc taaaatggaa
     2221 tatttagaaa cgatcaacat caatcatatt gctccaaatc cgtatcaacc tcgcctagaa
     2281 tttaatacaa aagaactaga agaattagcc gactctatta aaattaatgg ccttattcaa
     2341 cccattattg taagaccgtc agcagtcttt ggttacgaac tggtagccgg agaacgacgg
     2401 ttaagagccg caaagttagc taaattggaa agtatacctg caattataaa atcctataat
     2461 aatgatgata gtatgcaatt agctattgtc gaaaatttac aacgatctaa tttgtcaccc
     2521 attgaagagg ctaaagccta ctctcaactt ctacaaaaga aaagtatgac acatgaagag
     2581 ttagcaaaat atatgggaaa atctcgtccc tatatatcaa atacgatacg actactaaat
     2641 ttaccgccac taattacttc agctattgaa gaaggaaaac tttcctctgg tcatgcgcgt
     2701 gcactactca gtctaccaga tgctagtcaa caaaaagatt ggtatcagag aatcctcact
     2761 gaagatatta gtgtaagacg actagagaag ttattaaaac aagaaaagaa aacaaatcat
     2821 aagtcacttc aaaataaaga tgtctttcta aaacatcaag aaaatgagtt agctcaattt
     2881 ctaggtagca aagtgaaact tactattaat aaagatggtg ctggcaatat aaagattgct
     2941 ttcaataacc aagaagaatt aaacagaatt atcaacactc taaaataagg gtgttgatta
     3001 tttttttatt taatcaaact tatccacaag gtattttgct atttttctgt tgattctcta
     3061 aacttttcta atttccacag tctgtggaaa actttaatta acattgttga ttttattctt
     3121 caacatctgt ggaaaacttt atttttttat ggtacaatat aacaataatt atccacaaga
     3181 caataaggaa gaagctatga cggaaaacga acaactattt tggaatagag tactagagct
     3241 atctcgttct caaatagcac cagcagctta tgaatttttt gttctagagg ctagactcct
     3301 caaaattgaa catcaaactg cagttattac tttagataac attgaaatga aaaagctatt
     3361 ctgggaacaa aatttggggc ctgttatcct aacagctggt tttgaaattt tcaatgctga
     3421 aattacagct aactatgtct caaacgattt acatttacaa gaaactagtt tttctaacta
     3481 ccagcaatct agcaacgaag taaatacttt accaattaga aaaatcgact ctaaccttaa
     3541 agagaaatat acttttgcta attttgttca aggagatgaa aatagatggg ctgtttcagc
     3601 atcaattgct gtagctgata gtcctggcac gacttataat cctctattta tctggggggg
     3661 acctggtcta ggaaagacgc atctactaaa tgctattgga aatcaagttt taagagataa
     3721 tccaaacgcg agggttttat acatcactgc tgagaatttt attaatgaat ttgtcagtca
     3781 tattcgttta gattcgatgg aagaattaaa agaaaagttt cgcaacttgg acttactcct
     3841 gattgatgat attcagtcgc ttgctaagaa aaccttaggg gggacccaag aggagttctt
     3901 caatactttc aatgctttac atacaaacga taaacaaatc gtattgacca gtgaccgaaa
     3961 tccaaatcaa ttaaatgatc tagaagaacg tctagtcacg cgctttagtt ggggactccc
     4021 agtaaatatc acaccacctg attttgaaac acgagttgct attttaacca ataaaattca
     4081 agaatatcct tatgattttc ctcaagatac cattgaatac ttagcaggag aatttgattc
     4141 caacgtacgt gaattagaag gagccttgaa aaatattagt ctagttgctg actttaagca
     4201 tgctaaaact attacagtag atatagctgc agaagctatc agagcacgta aaaatgacgg
     4261 tcctattgtt actgttattc ctatagaaga aattcaaata caagttggta aattctatgg
     4321 cgtaactgta aaagagataa aagcaactaa aagaacacaa gatattgtcc ttgcaagaca
     4381 ggtagccatg tacttagctc gtgagatgac agataacagt ctccccaaaa taggtaaaga
     4441 atttggggga cgagatcact caactgttct ccacgcttat aataaaataa aaaatatggt
     4501 tgctcaagat gacaacttac gaattgagat agaaactatc aaaaataaaa tcaggtaggc
     4561 tttgtggata agtatcagaa aaacacgaga cttttccaca ggttgtgaac aagttatttt
     4621 ccttatgaat actaaa
//
LOCUS       DATROQ010000117         4565 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.118, whole genome shotgun sequence.
ACCESSION   DATROQ010000117 DATROQ010000000
VERSION     DATROQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.118"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            91..1416
                     /locus_tag="VBC94_001861"
     CDS             91..1416
                     /locus_tag="VBC94_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6980100.1"
                     /translation="MKKRILSAVLVSGVTLGTAAVTVNADDFDSKIAATDSVINTLSG
                     QQAAAQNQVTAIKGQVGALESQQSELEAQNAQLEAVSQQLGQEIQTLSNKIVARNESL
                     KKQVRSAQKGNLTNYINTILNSKSVSDAVNRVVAIREVVSANEKMLAQQEADKAALEA
                     KQIENQNAINTVAANKQAIENNKAALATQRAQLEAAQLELSAQLTTVQNEKASLIQAK
                     AQAEEAARKAAEAQAVAEAKAQAESVAKAQAAAQVESATAPTETVQTQPRTEIKPSNL
                     TATSSATTVAKTTATATNEPKVTQPSVVTKAVEAPKAVVSSTPRAVSKPVVRSYDSSN
                     TYPMGQCTWGAKSMASWVGNYWGNANQWGASARAAGYSVGTTPRVGAVAVWPYDGGGY
                     GHVAVVTSVANNSSIQVMESNYAGNMSIGNYRGSFNPSASGSVYYIYPN"
     gene            1540..2508
                     /locus_tag="VBC94_001862"
     CDS             1540..2508
                     /locus_tag="VBC94_001862"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO6980101.1"
                     /translation="MSYSNLKLFALSSNKELAKKVSQTIGIPLGQSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAESVSVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRQ
                     GLVGDDVVVVSPDHGGVTRARKLAQCLKTPIAIIDKRRSVTKMNTSEVMNIIGNIKGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQNSAIEKLIVL
                     DTIYLPEERLIDKIEQISIAELIGEAIIRIHEKRPLSPLFEMNKLK"
     gene            2616..3791
                     /locus_tag="VBC94_001863"
     CDS             2616..3791
                     /locus_tag="VBC94_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6980102.1"
                     /translation="MTLEKRFNKYLDRIEVSLIRQFDQSISDIPGMVKLTLGEPDFTT
                     PDHVKEAAKSAIDANQSYYTGMSGLLALRQAAADFAKDKYNLTYNPDSEILVTIGATE
                     ALSASLIAILEAGDVVLLPAPAYPGYEPIVNLVGADIVEIDTRENDFRLTPEMLETAI
                     IQQGEKLKAVLLNYPTNPTGITYSRQEIAALAEVLKKYDIFVISDEVYSELTYTGQQH
                     VSIAEYLPNQTILINGLSKSHAMTGWRVGLVYAPEAFTAQIIKSHQYMVTAASTISQF
                     AGVEALSVGKNDTLPMRQGYIKRRDYIIDKMSKLGFKIIKPSGAFYIFAKIPDSYPQD
                     SFKFCQDFAYQQAVAIIPGVAFGKYGEGYIRLSYAASMEVIETAMARLKVFMESYEG"
     gene            3781..4542
                     /gene="recO"
                     /locus_tag="VBC94_001864"
     CDS             3781..4542
                     /gene="recO"
                     /locus_tag="VBC94_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEO6980103.1"
                     /translation="MRVSQTYGLVLYNRNYREDDKLVKIFTETEGKRMFFVKHASKSK
                     FNAVLQPLTIAHFILKINDNGLSYIDDYKEVLAFQETNSDLFKLSYASYITSLADVAI
                     SDNVADAQLFIFLKKTLELIEDGLDYEILTNIFEVQLLERFGVALNFHDCVFCHRAGL
                     PFDFSHKYSGLLCPNHYYKDERRNHLDPNMLYLINRFQSIQFDDLQTISVKPEMKLKI
                     RQFLDMIYDEYVGIHLKSKKFIDDLSSWGSIMKSD"
BASE COUNT         1461 a          728 c          906 g         1470 t
ORIGIN      
        1 aaatataaat gtaacaatga cgtaatataa tttcgtgaat tttttggtaa aatatttaat
       61 gtcttatcgt aaaggagtag tttaataaaa atgaaaaaaa gaatattatc agcagtcctt
      121 gtgagtggag taactcttgg aactgctgcc gtaacagtaa acgctgatga ctttgactcg
      181 aaaattgctg ctactgattc agtaatcaat actttatcag ggcaacaagc agcagcacaa
      241 aaccaggtga ctgctattaa aggacaagtt ggtgctttag agtctcaaca atcagaatta
      301 gaagcgcaaa atgctcaact tgaggcagta tctcagcagt taggtcaaga gattcaaact
      361 ctttctaata aaattgttgc acgtaacgaa tctttgaaaa agcaagttcg tagtgctcaa
      421 aagggtaact tgactaacta tattaatact attttaaatt cgaaatcagt atctgatgct
      481 gttaatcgtg ttgttgctat tcgtgaggtt gtttcagcta atgaaaagat gttagcacaa
      541 caagaggctg acaaagcagc cttagaagct aaacaaattg aaaatcaaaa tgctataaat
      601 actgtagcag ctaacaagca agctatcgaa aataataaag cagccttagc aacacaaaga
      661 gcacaattag aagcagctca attagagtta tctgctcaat tgactacagt ccaaaatgaa
      721 aaagcatctt taattcaggc aaaagcacaa gctgaggaag ctgcgcgtaa ggcagctgaa
      781 gcacaagcag tagctgaagc gaaagcacaa gctgagtctg tagcaaaagc acaggcagca
      841 gcacaagttg agtctgccac agctcctact gaaacagttc aaacacaacc gagaactgaa
      901 ataaagcctt ctaatcttac ggctacttct tcggcaacta ctgttgctaa aactacagct
      961 acagctacta atgagccaaa agttactcaa ccttctgtag taactaaagc agttgaagca
     1021 cctaaagcag ttgtttcttc aacacctaga gcggtatcaa aacctgttgt cagaagttat
     1081 gattcaagta acacataccc aatgggacaa tgtacttggg gagctaagtc aatggcttct
     1141 tgggttggta actattgggg aaatgctaat cagtggggtg ctagtgcacg tgcagctggt
     1201 tattctgtag gaactacccc tcgtgttggt gcagtagctg tttggccata tgatggtggt
     1261 ggttacgggc acgttgcagt agtaacgtca gtggctaaca attcatctat ccaagttatg
     1321 gaatcaaact atgctggaaa tatgagcatt ggcaattacc gtggttcatt taaccctagt
     1381 gcttctggct ctgtttacta tatttaccca aattaatgaa tactaaggag atctttcgtg
     1441 gtctcctttt tgtttattaa ttaggtatgg tttattttgg attgaaaaat tgtgtaaaaa
     1501 gcgttaaaat ggtataggac taaagttttg gaggaaatca tgtcttattc taatttaaaa
     1561 ttgtttgcac tgtcatcaaa caaagaacta gcgaaaaaag tgtctcagac gattggaatt
     1621 ccgctaggtc aatcgacagt tcgccaattt tctgatggtg agattcaagt taatattgaa
     1681 gagtctattc gtggccatca tgtttttatt ttacaatcaa caagctcacc agttaatgat
     1741 aatttgatgg aaattttaat tatggttgat gctttaaagc gtgctagtgc ggagtctgtt
     1801 agtgtggtaa tgccttacta tgggtatgct cgtcaagatc gcaaggcacg ttctcgtgaa
     1861 cctattactt cgaaactcgt tgcgaacatg ctagaagttg ctggtgttga tcgtctttta
     1921 acagttgatt tacatgcagc tcaaattcag ggattctttg atattcccgt tgatcacctt
     1981 atgggggctc cccttattgc agattacttt gatcgtcagg gattagttgg tgatgatgtt
     2041 gttgtcgtta gtcctgatca tggtggcgta acacgtgcac gtaagctggc acagtgttta
     2101 aagacaccga ttgctatcat tgataagcgc cgtagtgtta ctaaaatgaa tacatctgaa
     2161 gtaatgaata ttattggtaa tatcaaaggt aaaaaatgta ttttaattga tgatatgatt
     2221 gatactgcag gtactatttg ccatgctgca gacgctttag ctgaagcagg ggcaacagct
     2281 gtttatgctt catgtactca ccctgtttta tcagggccgg ctttggataa tattcaaaat
     2341 tcagctattg aaaaattaat tgtgttagat acgatttacc tccctgaaga acgtttgatt
     2401 gataagattg aacaaatttc aattgcggaa ttgattggtg aagctattat ccgtattcac
     2461 gagaaacgtc ctttatcacc attatttgaa atgaataaac taaaatgata aaaaacctgt
     2521 tattaacagg ttttttatca tttaatgacg gaaaactgga cacaaattat ttttcttgta
     2581 taattaaata tattatttct tatcaggagg ttatgatgac attagagaaa cgatttaata
     2641 agtacttaga tagaatagag gtctccttaa ttcgtcagtt tgatcaatct atttcagata
     2701 tacctggaat ggtaaaatta actcttggag aaccagattt taccactcct gatcatgtta
     2761 aagaagcagc taaatcagct attgatgcga atcaatcgta ttatactggt atgagcggtt
     2821 tattagctct aaggcaagct gctgcagatt ttgcaaagga taaatataat cttacttata
     2881 accctgatag tgagatcttg gttacgatag gtgccacaga agctctgtct gcatctctta
     2941 tagctatttt agaagctggt gatgttgttc tattacctgc ccccgcttat ccaggctatg
     3001 agccaatagt taaccttgta ggagctgata tcgtagaaat tgatactaga gaaaatgact
     3061 ttagactaac ccctgaaatg ttagaaacgg ctattatcca acaaggtgaa aaattaaagg
     3121 ctgttttact taattatcct actaatccta caggtattac ctattcaagg caagaaattg
     3181 ctgcattagc tgaagtcctt aaaaaatatg atatttttgt tattagtgat gaagtttact
     3241 ctgagttaac ttacactggt cagcaacatg tttcaattgc agaatattta cctaaccaaa
     3301 ctatcttaat taatggctta tcaaaatcac atgctatgac tggttggaga gttggattgg
     3361 tgtatgctcc tgaagcattt actgctcaaa tcattaaaag tcatcaatac atggtgacag
     3421 ctgctagtac tatttcgcaa tttgctgggg tagaagcctt atctgtgggt aaaaatgata
     3481 cgttacctat gcgtcaagga tatattaaac gtagagatta tatcattgat aaaatgtcta
     3541 aacttggttt caaaataatt aaaccgagcg gcgcttttta tatctttgct aaaatacctg
     3601 atagttatcc acaggattct ttcaagtttt gtcaggattt tgcttatcaa caagctgtag
     3661 ctattattcc aggggtggct tttggtaaat atggtgaagg gtatatacgt ctttcttatg
     3721 ctgctagtat ggaagttatt gaaacagcaa tggctagatt gaaagttttt atggaatcat
     3781 atgagggtta gtcaaacata cggtctcgtt ttgtataatc gtaattatcg tgaagacgat
     3841 aaattagtta aaatctttac ggagactgag gggaaaagaa tgttttttgt aaagcatgct
     3901 tcaaagtcaa aattcaatgc tgtgctccaa cctttgacta ttgctcattt tattttaaaa
     3961 attaatgata atggtctttc ttatattgat gattataaag aagttttagc atttcaagaa
     4021 accaattcag acttgtttaa gttgtcatat gcgagttata ttacttcttt ggctgatgtg
     4081 gctattagtg ataatgtagc ggatgctcaa ttatttattt tcctaaagaa aacgttagaa
     4141 ttgattgagg acggtttaga ttatgagatt ctaacgaata tttttgaagt acaactacta
     4201 gagaggttcg gtgttgcttt aaattttcat gattgtgttt tttgtcatag ggcaggatta
     4261 ccttttgatt tttcacacaa atattctgga ttattatgtc caaaccatta ttataaagac
     4321 gagagaagaa accacctaga tcctaatatg ctgtacttaa tcaatcgttt tcagtcaatt
     4381 caatttgatg atttacaaac aatttctgtg aaacctgaga tgaaacttaa aattcgtcaa
     4441 tttttggaca tgatttacga tgaatatgta gggattcatc ttaaaagtaa aaaatttatt
     4501 gatgatttgt ctagttgggg aagtattatg aaatcagatt aagttgtaaa gaggaacatg
     4561 acatt
//
LOCUS       DATROQ010000118         4542 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.119, whole genome shotgun sequence.
ACCESSION   DATROQ010000118 DATROQ010000000
VERSION     DATROQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.119"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..1220)
                     /locus_tag="VBC94_001865"
     CDS             complement(<1..1220)
                     /locus_tag="VBC94_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO6980104.1"
                     /translation="MATSTENLQLAKRGPIISIIAYITLAVAKLAAGYWFDATSLVAD
                     GFNNLSDILGNVALLIGLHLASQPADSNHRFGHWKIEDLASLITSFIMFVVGIQVFIQ
                     TVTKIINNTDTNIDPLGAIVGAISALVMLGVCFYNKQLSQRVKSSALVAASKDNLSDA
                     VTSIGTSIAIIAASLNFPIIDRLAAIIITYFILKTAYDIFIESAFSLSDGFDDYQLKQ
                     YEKAILTIPKISAVKSQRGRTYGSNIYLDIVLEMNPDLSVFESHAITEKVEKLLSDKF
                     SVYDIDIHVEPASIPEDEIFDNVYQKLYKNEKIILAKIPGYETFISPDFYMINEKGNI
                     ITSDMLTNATNHSLASNFKYFNVKSISQKTKLVSYELEGKRHTSIWRRNEKWFLIYHQ
                     ITAKSSPYKTRRYQ"
     gene            complement(1284..2630)
                     /locus_tag="VBC94_001866"
     CDS             complement(1284..2630)
                     /locus_tag="VBC94_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEO6980105.1"
                     /translation="MLTLFTHINSFVWGPPLLALLVGTGIYLSFRLGFVQLRQLSRAF
                     KLIFREDNGQGDISSYAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGLLAIKYRTKDTNGEISGGPMYYIVNGMGQKWKPLAVFFSAAGILVALLGIG
                     TFTQVNAIASSLEHTFKISTRFTSLILAVIVLFIIFGGIKSISKVSEKIVPFMAISYI
                     LATLIIIAVNYNKIPHTFQLIFSGAFSGTAAIGGFSGAIVKEAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTKEPVEQGLISMTGTFIDTIVICTLTGIAILVTGKWLEFDLQGAPL
                     TQASFNTVFGSLGSFALTFCLVLFAFTTILGWSYYGERCFEYLLGTKFINAYRIIFVI
                     MVGLGGFLQLDLIWVIADIVNGLMALPNLIALLALSPIIVKETQKYFSETKNITKP"
     regulatory      complement(2787..2919)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3227..>4542
                     /locus_tag="VBC94_001867"
     CDS             3227..>4542
                     /locus_tag="VBC94_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEO6980106.1"
                     /translation="MTLNAKGESNQILVDKEVHSTNLLLDIDEKPELFQGLLLSFQHV
                     FAMFGATILVPLILGMPVSVALFASGCGTLIYQVATKFKVPVYLGSSFAYITAMALAM
                     KQMHGDISAAQTGILFVGLIYVVVATVIKFVGNSWVDKILPPIIIGPMIIVIGLGLAN
                     SAVTNAGFVAKGDWRKMLVAVVTFLIAAFINTKGKGFIKIIPFLFAIIGGYILSIILG
                     LVDLSPVEKAAWFELPKFYLPFKTGLFHSYKLYFGPEMLAILPISIVTIAENIGDHTV
                     LGQICGRNFLKKPGLNRLLIGDGLATAFSALIGGPAETTYGENTGVIGMTRIASVTVI
                     RNAAFIAIAFSFFGKFTALISTIPSAVLGGMAILLYGVIASNGLKVLIENRVNFAEVR
                     NLIIASSMLVLGLGGAVLDLGALTLSGTALSAIVGIILNLILPKES"
BASE COUNT         1446 a          770 c          831 g         1495 t
ORIGIN      
        1 atttgatatc gtctcgtttt atacggactt gacttggcag ttatttgatg gtagattaag
       61 aaccattttt cattacggcg ccaaatacta gtatgtcgtt taccttccaa ttcataagac
      121 acaagttttg ttttttgact aattgatttt acattgaaat atttaaagtt gcttgccaat
      181 gaatgatttg tagcattagt aagcatgtca gaagtgataa tatttccctt ttcattaatc
      241 atataaaagt cagggctaat aaaagtctca taaccgggga tttttgctag gatgattttt
      301 tcgtttttgt ataatttttg gtaaacatta tcgaaaattt cgtcttctgg gatagaagcg
      361 ggttctacat gaatatctat gtcatataca gaaaatttat cactgagtag tttttccact
      421 ttttcggtaa tggcatgact ttcaaagact gagagatcag ggttcatttc gagaacaata
      481 tcaaggtaaa tattactacc gtaagtacgt ccacgttgag attttacagc ggatatttta
      541 ggaattgtta atatagcttt ttcgtattgt tttaattgat aatcatcgaa accatccgat
      601 aagctgaaag cagattctat aaaaatatca taagctgttt taagaataaa ataagtaata
      661 atgatagcag ctaaacgatc aataatagga aaatttagag aagccgcaat gatagcaata
      721 gatgtgccga tagatgtcac agcatcagaa aggttatctt tagaagctgc aaccagagcg
      781 ctagacttta ctctttgaga taattgttta ttatagaaac aaacgccaag cataacaaga
      841 gcagatatag cacctactat agcacctaga ggatcaatgt tagtatcagt attattaata
      901 atcttagtga cagtttgaat aaaaacttga atacctacga caaacatgat aaaagaggta
      961 atcaggcttg ctaaatcttc aattttccag tgaccaaaac gatggtttga gtcggctggt
     1021 tgactagcca agtgtaaacc aattaagagt gctacatttc ctaagatatc agataagtta
     1081 ttaaatccat ctgctacaag tgaagtagca tcaaaccaat aacctgcagc taatttagcg
     1141 actgccagtg ttatataggc aatgatacta ataataggcc ctctttttgc aagttgtaag
     1201 ttttcagtag aagttgccat tttcctaatt tcctttcatc tacacattat atcacttagg
     1261 agcagactat aaaactagta agttcaaggc ttagtgatat ttttagtctc tgaaaaatat
     1321 ttctgtgttt ctttaacaat aattggtgac aaagctagga gagctattag atttggtaaa
     1381 gccattaaac cattaacgat atcagcgata acccaaatga gatcgagctg taagaaacca
     1441 cctaagccga ccataatgac aaaaataatg cgataagcgt taataaattt agtccccaat
     1501 agatattcaa agcaacgctc tccgtagtag ctccatccta aaatagtagt aaaagcaaat
     1561 aagacaaggc agaaggttag ggcaaaacta cctagtgagc caaatacggt attaaaagat
     1621 gcttgtgtta acggagcacc ctgtaaatca aattctagcc attttcctgt aactaaaatt
     1681 gcaatccctg tcaaagtaca aataacaata gtgtcaatga aggttcctgt cattgaaata
     1741 agcccttgtt caacaggttc ttttgtttta gctgctgctg cagctattgg agcagaaccc
     1801 aatccagatt catttgagaa aactcctcta gcaattccct tttgaattgc ttctttaacg
     1861 atagctccag aaaaaccacc aatggctgct gtcccagaaa aagcaccgct aaagatgagt
     1921 tggaaagtat gtggaatctt attgtaatta actgcaataa taatcagagt tgctaaaata
     1981 tagcttatag ccataaaagg cactattttt tctgaaactt ttgaaataga ttttatacct
     2041 ccaaatataa taaataatac tataacagca agaatgaggc tggtaaatct agttgatatt
     2101 ttgaaagtat gctcaagaga agaggcaatt gcattgactt gagtgaatgt ccctattcct
     2161 aaaagagcta ctaagatacc tgctgcagaa aaaaagactg ctaatggttt ccatttttgt
     2221 cccattccat tgacaatgta gtacattggt cccccagaaa tttctccatt agtatctttt
     2281 gtgcgatatt tgatagctaa taatccttct gcgtattttg ttgccattcc aaaaaaggcg
     2341 gctacccaca tccaaaacaa agctcctggt cctccagatt taatagccgt agccacacca
     2401 acgatattac ctgtccctac cgttgcagca agagcagttg caagagcagc ataacttgaa
     2461 atatctcctt gtccattatc ttctcggaaa atcaatttga aagctctaga aagttgtctc
     2521 aattgaacaa aacctaagcg aaatgatagg taaatacctg ttccgactaa taaagcaagt
     2581 aaaggtggac cccaaacgaa gctattgata tgagtaaaaa gtgttagcat atgttctcct
     2641 ttgatacatg acatacctat ggattctgta atgaaacgaa agaagttatc tctaaaagag
     2701 ataacttctg aaagatgcta gctaaaaagc gcacaaataa aggtagtagt aaacttaata
     2761 agcttactac tcattattaa tattggcttt tagcttctgt ccttttgcct gagagattgg
     2821 aggagttgtt ttactcttct tgccccttcg gcgcctagag cacatcttgg tgtcaataca
     2881 aagatgttgc tacttatagg tctctccaga agtccgtctg tcaatcagtc ctgtcttaag
     2941 acacctgaga gtgtaattcc ttcggcgaaa gtctaacttt cttctcctga atgacgtcat
     3001 tcggtatatt ttattatgtt aactatcata atgaaaaagc atagttttgt caatatagtt
     3061 tcaaaaaact cttgttttct aaaatgtctt atgctataat tctttcagcg taggttttta
     3121 aactcatccc gtgaggtgag aaaaacatca ggaaaaaaat aaagcctatg actatattgc
     3181 tatagaaagt gggctttttt gtatgcccaa gaggactagt ttagatatga ctttaaatgc
     3241 taaaggtgaa agtaatcaaa ttctagtaga taaggaagta cattctacta acttattact
     3301 agatatcgat gaaaaaccag aattatttca aggtttgctt ttaagttttc aacatgtttt
     3361 tgccatgttt ggagctacga tattagttcc gcttatttta gggatgcctg tatcagtggc
     3421 tttatttgct tctggttgcg gaacgctcat ttatcaagtt gcgacaaaat ttaaagtccc
     3481 tgtttatcta ggctcatcat ttgcttatat tacagctatg gcattagcca tgaaacaaat
     3541 gcatggcgat atttcggctg ctcaaacagg tattcttttt gtaggtctaa tctatgtcgt
     3601 tgtggcgacg gttattaagt tcgtaggaaa ttcatgggtt gataagattt tgccaccaat
     3661 aatcattgga cctatgatta ttgttattgg tttaggtttg gcaaactctg ctgtaactaa
     3721 tgcaggtttt gtcgctaaag gtgattggcg taaaatgcta gttgctgttg ttacattctt
     3781 gattgcagct tttatcaata ctaaagggaa aggttttatc aagattattc catttttatt
     3841 tgcaattatt ggaggttata ttttatcaat cattcttggc ttggtagatt tatctccagt
     3901 tgaaaaagct gcctggtttg aattaccaaa attctattta ccgtttaaga caggtttatt
     3961 tcacagttat aaactttact ttggaccaga aatgcttgca attcttccaa tctcaattgt
     4021 aacgattgct gaaaatatcg gtgatcatac agttttaggt cagatttgtg ggcgtaactt
     4081 tctaaaaaaa ccaggtttaa atcgtctttt aattggagat ggtttagcaa ccgcattttc
     4141 tgctcttata ggagggccag ctgaaactac ttatggtgaa aatacaggtg ttattggcat
     4201 gacgcgtata gcttctgtaa cggttattcg taatgcagct tttattgcaa tcgctttttc
     4261 attctttggt aaatttacag ctttgatctc tacaattcct agtgcagtac ttggtggaat
     4321 ggctatatta ctttatggtg ttattgcaag taatggatta aaagtgttga ttgaaaatcg
     4381 tgtcaatttt gctgaggttc gtaatctgat tattgccagt tccatgttag ttttaggcct
     4441 cggtggagca gtccttgatc taggtgctct aactttatca ggaacagcat taagtgctat
     4501 agtgggtatt atattaaatt taatattgcc aaaagaaagc at
//
LOCUS       DATROQ010000119         4503 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.120, whole genome shotgun sequence.
ACCESSION   DATROQ010000119 DATROQ010000000
VERSION     DATROQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.120"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..1979)
                     /gene="secA2"
                     /locus_tag="VBC94_001868"
     CDS             complement(<1..1979)
                     /gene="secA2"
                     /locus_tag="VBC94_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEO6980107.1"
                     /translation="MKKVVVKPKISILNKLRLQRIQKIVNEINNKKVYYAQLSDKELS
                     HQTLILKNRLSSGESLDDILVDAFAVIREANKRILGLFPYDAQVMGAIALHQGTIAEM
                     KTGEGKTLTATMPLYLNALTSKGAILVTTNDYLAKRDALEMGKVYQFLGMTVGINVFD
                     KKEEADATIKREVYESDITYTTAGALGFDYLVHNLASNKLEQFLRPFHFVIVDEADSV
                     LLDIAQTPLIIAGDPRVQSNLYGITNNFVLTLKENDEYIHKAKDKIVYLTEKGVSYAK
                     QYFNISELYNDEYWELNRHINLALRAHCLYKRDYDYVVKNNEVKLLDNATGRVMEGTK
                     LQSGIHQAIEAKEDVALTNESRAIASITYQSLFNMFPRLSGMTGTGKSSEDELIKTYH
                     LPVIVIPTNFPIKRVDLADKIYVSLPEKLQATIDDVKQRHSKGQPVLLISGTVEIANI
                     YSKLLLREGIAHNTLTADNVVKEAQIIKEAGQKGSVTCATVLAGRGTDIKLGEGVREL
                     GGLAVIGTERMPNLRMDWQLRGRSGRQGDPGISQFYVSLEDELIVSHSPEWVIKYLKK
                     YSRKETSNYYMVPRKKRFFYQIVKNAQLRSEDKGVSSREQTIKFGESLRIQRENIYQL
                     RNELIADSSIVVDGVIKIIQDNFNDIANDKDLTEHS"
     gene            complement(1976..2521)
                     /gene="asp3"
                     /locus_tag="VBC94_001869"
     CDS             complement(1976..2521)
                     /gene="asp3"
                     /locus_tag="VBC94_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEO6980108.1"
                     /translation="MIKKRIQKVLWTDSSNVYLYGSTIKYLEHNRVFFENLRFSTGKT
                     IKKWLSKTNFQANRTLPELTLLKPNHTYELVSKLTTVPEGCVIIQINFYNRQKEKIGF
                     SIIRDHSSDFIVPDDTYFYDISLIGAGCISVEFCYLELFEVIETQEEKDGIELNSYVE
                     SSLPSELELIRTLIAFSKDKK"
     gene            complement(2514..4103)
                     /gene="asp2"
                     /locus_tag="VBC94_001870"
     CDS             complement(2514..4103)
                     /gene="asp2"
                     /locus_tag="VBC94_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEO6980109.1"
                     /translation="MEKLKILQIGEEPWDADLITPDNIEWLYCNGNAIQIFLEKLKSK
                     ELEKNNVEYIGKTSTNEVKLWFNAVILTSEVSELQLNLLSDCLEAHTLFYDEDFQLDF
                     CSENGIFKRKVLRPLPFNGSRKEKIIFLSRTLFSEQYGAKLKVSDIDLNPDFKGEIIY
                     EGNSSICFSGNFGEDFQPLFSFRYNLSSTDKAIEIWQEYQKLQGDNSIMIEVIQYQKG
                     SLDLILNTKYLSENDLADPYILEYDESVGFSSISIFARGDGVVRFGALHWRYSRKGLG
                     QFLIGGERYSDSKRQEFIYYFNPGDMKPPLNVYFSGFRGAEGFEGFYMMKRLGAPFLL
                     IGDPRIEGGSFYLGTSEYESELENVLQKYLDYLQFDHCQLILSGLSMGSFGALYYGST
                     FSPHAIVVGKPFTNLGDMVVNLKLRRPDDFETSMDILQNIIGSQNDEAINQFNQKFWN
                     KFKRSNFEKTQFAISYMKHDDYDRNATPRLIDYFSKTNTLLYASGYAGRHNDNSQSIN
                     NWFTSQYKTILSDDFGRVFSD"
     gene            complement(4105..>4503)
                     /gene="asp1"
                     /locus_tag="VBC94_001871"
     CDS             complement(4105..>4503)
                     /gene="asp1"
                     /locus_tag="VBC94_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEO6980110.1"
                     /translation="SLSLEDENHFKEEQKTKIRGISFDIYTNENQIIDALDTARLVID
                     LRDDPDLYTQIAALSSGIPQINKVHTNYVEHQKNGWIISDSTELNNAIDYYFDGLMNW
                     NKSLVYSVKKMGDYTSGKILQRWRKLLEKE"
BASE COUNT         1447 a          867 c          606 g         1583 t
ORIGIN      
        1 gaatgttccg ttaaatcctt atcattagca atatcgttaa aattatcctg aataattttt
       61 atcacaccat caaccacaat ggaactatca gctattaatt catttcgtaa ttgataaata
      121 ttttcacgct gtatacgcaa gctctctccg aacttaatag tttgctccct agaagataca
      181 cctttatctt cacttctgag ttgagcattt ttaactattt gataaaaaaa gcgtttcttt
      241 ctaggtacca tatagtaatt tgaagtttct ttacgagaat atttttttaa atactttatc
      301 acccattccg gagaatgaga aacaattaat tcatcctcta gcgacacata aaattggcta
      361 atccctgggt ctccttgacg ccccgaacgg cccctaagct gccagtccat acgtaaatta
      421 ggcatccttt ccgttcctat aactgcaagt cctcctaatt ctctaacccc ttcacctagt
      481 ttaatatctg ttccacgacc agcaagaact gttgcacatg ttactgaacc tttttgtccc
      541 gcttctttta taatttgtgc ctctttaaca acattatcag ctgttaaggt attatgggca
      601 atgccttctc gcagcaataa ctttgaataa atgttagcaa tttcaactgt tccagaaatt
      661 aacaatactg gctgaccttt actatgtctt tgtttaacat catctatagt agcttgcaac
      721 ttttctggta agctaacata tattttatcg gcaagatcta ctcgtttgat cggaaaattt
      781 gttggtataa caataacagg caggtggtaa gtttttataa gttcatcttc agaagatttt
      841 ccagttcctg tcatcccaga caaccttggg aacatattaa agagactttg atacgttatt
      901 gaagcaattg ccctagattc atttgtcaaa gcaacatctt cttttgcttc aatagcttgg
      961 tgaatccctg attgaagttt tgtgccttcc ataacacgac ctgttgcgtt atctaacaat
     1021 tttacttcat tatttttaac tacataatca taatctcttt tatacaaaca atgagcacgc
     1081 aatgctaggt ttatatgtct atttaattcc caatactcat cattatataa ttctgaaata
     1141 ttgaagtact gttttgcgta acttacaccc ttctcagtaa gatagacaat tttatcctta
     1201 gctttatgga tatattcatc attttcctta agagtgagta caaaattatt agtgatacca
     1261 tagagattag attgtacacg aggatctccc gctataatta agggtgtttg agcaatatct
     1321 aacaaaacag agtctgcttc atcaacgata acaaaatgaa aaggtcttag aaattgttct
     1381 agtttattac tagccagatt gtgaacgaga tagtcgaaac ctaaggcacc tgcagtagta
     1441 taagtaatat cagattcata tacttctctc ttaatagtcg catctgcttc ttctttttta
     1501 tcaaaaacat taataccaac tgtcatacct agaaattgat atacttttcc catctctaat
     1561 gcatctcgtt ttgccaaata atcattggtc gtcactaaga ttgctccctt gctagttaaa
     1621 gcattcagat acaagggcat agttgcagtt aatgtttttc cttctcccgt tttcatttca
     1681 gctatcgttc cttgatgaag agcaatagca cccataacct gagcatcata tggaaacaaa
     1741 cctaaaattc ttttattagc ctctcttatc acagcaaaag catcaacaag aatatcatct
     1801 agactttctc cacttgaaag tctatttttt aatattaaag tttgatggct taactcctta
     1861 tctgataatt gagcgtagta cactttttta ttatttatct cgtttacaat tttttgaatt
     1921 ctttgcaatc tcaatttatt tagaatagag attttgggtt taacaacaac ttttttcatt
     1981 ttttatcctt agaaaatgct atcaacgttc taataagttc aagctctgat ggtaagctgc
     2041 tttcaacata actgtttagt tcaataccat ctttttcttc ttgagtctca attacttcaa
     2101 ataattctag gtaacaaaat tctacactta tacaacccgc accaataaga gaaatatcat
     2161 aaaaatatgt gtcatcagga actataaaat ctgaggagtg atctcgaata atagaaaaac
     2221 ctattttttc tttttgacga ttataaaaat ttatctgaat aatcacgcat ccctcaggca
     2281 cagtagttaa ttttgatacc aactcatagg tatgattagg ttttagtaag gtaagctctg
     2341 gcaacgttct attagcttga aaattcgttt tagaaagcca ttttttgatt gtttttcctg
     2401 tagagaatct tagattctca aaaaaaacac gattatgctc caaatacttt atagtcgaac
     2461 cataaagata aacattgcta ctatcagtcc aaagcacctt ctgtattctt tttttaatca
     2521 ctaaacactc tcccaaaatc atcactgaga attgttttat actgagaagt aaaccaatta
     2581 tttattgatt gactattatc attatgtcgc ccagcatatc cacttgcata caacagggtg
     2641 ttagttttag aaaaataatc aatcagcctt ggagttgcat ttctatcata atcatcatgt
     2701 ttcatataag atatagcaaa ctgcgttttt tcaaaattag aacgcttaaa cttattccaa
     2761 aatttttgat taaattgatt aatagcttca tcgttttgag aaccaataat attttggaga
     2821 atatccatac tcgtttcaaa atcatctggt cttcttaatt ttaaatttac taccatatcg
     2881 cctaaatttg taaagggttt ccccacaact atagcatggg gggaaaaagt agaaccataa
     2941 taaagggcac caaaacttcc catagataaa cctgataaaa taagttgaca atgatcaaat
     3001 tgtaggtaat ctaaatattt ttgtaaaaca ttttctaatt cactttcgta ttctgaagta
     3061 cctaggtaga agcttccacc ttcaatcctt ggatcaccaa ttaagagaaa cggagctccc
     3121 aaacgtttca tcatgtaaaa accctcaaaa ccttcggctc ctcgaaaacc tgaaaaataa
     3181 acattcaatg gaggtttcat atctccaggg ttaaaatagt agataaactc ttgccttttg
     3241 gaatcactgt acctttctcc accgattaaa aattgaccca aacctttacg cgaatatcgc
     3301 caatgtagtg caccaaatct caccactccg tcacctctag caaagataga aattgatgaa
     3361 aaaccaactg attcatcata ttctaaaata tatggatcag ccaaatcatt ttctgaaagg
     3421 tattttgtat ttaatatcaa atcaagtgat cctttttggt attgaatgac ttctatcata
     3481 atactattat caccttgcaa tttttgatat tcttgccata tttctatcgc tttatcagta
     3541 gaggataaat tatagcgaaa tgagaataga ggttgaaaat cttctccaaa attccctgaa
     3601 aaacaaatag agctattgcc ttcatatatt atctctccct taaaatcagg atttagatct
     3661 atgtcagaaa ccttcaattt tgcaccatat tgttccgaaa aaagagtacg gcttaggaaa
     3721 ataatttttt ctttccttga tccattaaat ggtagtggtc tcaaaacctt acgtttaaat
     3781 ataccgtttt ctgagcaaaa atcgagttga aaatcctcgt cgtaaaagag tgtatgcgct
     3841 tctaagcaat ctgaaagtag gttcaattgc aattcgctaa cctcactagt taaaatgacc
     3901 gcattaaacc ataatttaac ttcattagtt gaagttttac caatatattc aacattattt
     3961 ttttctaact ctttactttt taatttctct aagaaaattt gaatagcatt tccattacag
     4021 tataaccatt caatattgtc tggagttatt aaatcagcat cccatggttc ctcaccaatc
     4081 tgcaaaattt ttaatttttc catattattc tttttctaat aattttcgcc agcgctgtaa
     4141 tatcttaccg cttgtataat cacccatttt ttttacgctg taaactaacg atttattcca
     4201 attcattagt ccatcaaaat aatagtcaat tgcattattg agttctgtag agtcactaat
     4261 aatccagcca ttcttctggt gttctacata attagtgtga actttattga tttgaggaat
     4321 tccagaactc agagcagcaa tttgagtata taggtctgga tcatctctta aatcaataac
     4381 taacctagca gtatctagag catctataat ttgattttca tttgtatata tatcaaaact
     4441 aatgccacga attttagttt tttgttcttc cttaaaatga ttctcatctt ccaaagaaag
     4501 aga
//
LOCUS       DATROQ010000120         4491 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.121, whole genome shotgun sequence.
ACCESSION   DATROQ010000120 DATROQ010000000
VERSION     DATROQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.121"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            5..78
                     /locus_tag="VBC94_001872"
     tRNA            5..78
                     /locus_tag="VBC94_001872"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Asn,seq:gtt)
     gene            218..1069
                     /locus_tag="VBC94_001873"
     CDS             218..1069
                     /locus_tag="VBC94_001873"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEO6980111.1"
                     /translation="MKIFEKAPAKLNLGLDIKGRCDDGYHELAMIMVSIDLNDYVTIS
                     ELKEDCIVIDSDSSKMPLNNDNDVFKAADIIKNQYGINKGVHIRLEKSIPICAGLGGG
                     STDAAATIRALNRLWNLQMGHDEMVAIGFKIGSDVPYCLGGGCSLVLGKGEIVKPLPT
                     LRPCWIVLVKPDFGISTKSIFRDIDCKSISRVDIDLLKSAILSSDYQLMVKSMGNSLE
                     DITITKNPVISTIKERMLNSGADVALMTGSGPTVFSMCSTEKKADRVFNSMKGFCKEV
                     YKVRLLR"
     gene            1156..1599
                     /locus_tag="VBC94_001874"
     CDS             1156..1599
                     /locus_tag="VBC94_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEO6980112.1"
                     /translation="MTVLEQKLDHLVSQILLKAENQHELLFGTCQSDVKLTNTQEHIL
                     MLLSQEQLTNSDLAKKLNISQAAVTKAVKSLISQDMLKANKDSKDARITYFELSELAK
                     PIADEHTHHHDNTLGVYGRLVNHFSKDEKVVLERFLDLFSRELEG"
     gene            1602..2312
                     /locus_tag="VBC94_001875"
     CDS             1602..2312
                     /locus_tag="VBC94_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO6980113.1"
                     /translation="MRYITVSGLTFQYDSDPVLEGVNYHLDSGEFVTLTGENGAAKST
                     LIKATLGILTPKVGTVNISKENKEGKKLRIAYLPQQIASFNAGFPSSVYEFVKSGRYP
                     RNGWFRRLTKHDEEHIRVSLEAVGMWDNRHKKIGSLSGGQKQRAVIARMFASDPDIFV
                     LDEPTTGMDAGTTEKFYELMHHNAHKHGKSVLMITHDPDEVKGYADRNIHLVRNQSLP
                     WRCFNVHTNEMEVESDAT"
     gene            2302..3114
                     /locus_tag="VBC94_001876"
     CDS             2302..3114
                     /locus_tag="VBC94_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO6980114.1"
                     /translation="MLLDMLSYDFMQRALLAVVAISIFAPILGIFLILRRQSLMSDTL
                     SHVSLAGVALGVVLGISPTWSTIFVVTLAAVVLEYLRTVYKHYMEISTAILMSMGLAI
                     SLIVMSKAHNVGNVSLEQYLFGSIITIGKEQVIALFVIALITFILTILFIRPMYILMF
                     DEDTAFVDGLPVRTMSILFNVVTGIAIALTIPAAGALLVSTIMVLPASIAMRLGRNFK
                     TVIFLGMLIGFVGMVAGIFLSYYWETPASATITMIFIGIFLLVSLVGLLRKR"
     gene            complement(3325..4355)
                     /locus_tag="VBC94_001877"
                     /pseudo
     CDS             complement(3325..4355)
                     /locus_tag="VBC94_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767962.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
BASE COUNT         1301 a          657 c          886 g         1647 t
ORIGIN      
        1 ataatccgcc atagctcagt tggtagtagc gcatgactgt taatcatgat gtcgtaggtt
       61 cgagtcctac tggcggagta gataaaggag actaactttg gttagccttt ttttattgga
      121 attattattc cttaaggtcg gctagcatct taattgtccg aatttgtcat ttgttatgtt
      181 ataatatgga tattctatat tttattaggg aggttttatg aaaatttttg agaaagctcc
      241 tgccaaactt aatttaggat tagatattaa aggacgatgt gacgatggtt atcatgaatt
      301 agctatgatt atggttagta ttgatcttaa tgactacgtt accatttctg agctaaagga
      361 agattgtatt gttatcgatt ctgatagtag caaaatgcct ttaaataatg ataatgatgt
      421 gtttaaagct gcagatataa tcaaaaatca atatggtatt aataaaggtg ttcatattag
      481 attagaaaaa tccattccta tctgtgcagg actggggggg gggtctactg atgctgctgc
      541 tacaataaga gctcttaatc gattatggaa tcttcaaatg ggccatgacg aaatggttgc
      601 tattggtttt aaaattggta gtgatgttcc ttattgcctt ggtgggggat gttcgttagt
      661 attaggtaaa ggtgaaattg ttaaaccctt accgacattg agaccatgtt ggatagttct
      721 ggttaagccg gattttggta tttcaactaa atccattttt agagatattg attgtaagtc
      781 tataagcaga gttgatattg atttattaaa gtctgctatt ttgtcatcag attatcagtt
      841 aatggtcaaa tctatgggaa attcactcga ggatattaca attactaaaa atccagttat
      901 aagtacaata aaagaacgta tgttaaattc tggagccgat gttgcattaa tgacaggaag
      961 tggaccaact gttttttcaa tgtgttctac agagaaaaaa gctgatcgtg tttttaatag
     1021 tatgaaaggt ttttgcaaag aagtttataa agttaggttg ttgagataaa aggttttatc
     1081 ttcttcacct atctatgatt gattcttcta tatcttcgtg atataattta ctggttaaca
     1141 aaaaaagagg tatatatgac agttttagaa caaaaattag accatttagt gagtcaaatc
     1201 ttgcttaagg cagaaaatca acatgaatta ttgtttggta cttgtcaaag tgatgtaaaa
     1261 ttgactaata cgcaagaaca cattctgatg cttttatcac aagagcagtt gacaaattct
     1321 gatttagcta agaagttaaa tattagtcag gcagctgtaa ctaaagcagt aaaaagttta
     1381 atttctcagg atatgttgaa agcgaataaa gattctaaag atgctcgtat tacttatttt
     1441 gagttatctg aacttgctaa acccattgca gatgaacaca cacatcatca tgacaatacg
     1501 ttaggggttt acggaagatt agtaaatcat ttttcaaagg acgaaaaggt cgttttagaa
     1561 cgttttcttg atcttttctc tagagaatta gaagggtaat catgcgatat attacagtta
     1621 gtggtcttac ttttcagtat gatagcgatc ctgtgctgga aggggttaat taccatttag
     1681 acagtgggga gtttgttacc ttaacaggtg aaaatggggc tgctaaatca actttaataa
     1741 aggctacttt aggtatttta acgcccaaag tgggtacagt taatatctct aaagaaaaca
     1801 aagaaggaaa aaaattacgt attgcctatt tacctcaaca gattgcaagt tttaacgctg
     1861 gctttccatc gtcagtttat gaatttgtga aatctggacg ataccctaga aatggttggt
     1921 ttagaagact gactaaacat gatgaagaac atattagggt tagtttagaa gctgttggta
     1981 tgtgggataa tcgccataaa aagataggaa gtttatctgg cggtcaaaag cagagggctg
     2041 tcattgctag gatgtttgct tctgatccag atatttttgt attggatgag ccaacaactg
     2101 gtatggacgc agggactact gagaaatttt atgagttaat gcatcataat gctcataagc
     2161 atggtaagtc tgttttgatg attactcacg atcctgatga agtaaagggg tatgctgacc
     2221 gaaatattca tcttgttagg aaccaatctt taccatggcg ttgtttcaat gtccatacaa
     2281 acgaaatgga ggttgaatca gatgctactt gatatgcttt cctatgattt tatgcaacga
     2341 gcgctcttag cagttgtggc tattagtatt tttgctccaa ttttaggcat cttcctaatt
     2401 ttacgtcgtc agagtttgat gagtgatact ttgagccacg tttccttagc cggtgttgca
     2461 ttaggcgttg ttttaggtat ctcgcctacg tggtcaacta ttttcgttgt tactttggcg
     2521 gcagttgtat tagagtattt gcgtactgta tacaaacatt atatggaaat atcaactgct
     2581 atcctgatgt cgatggggtt ggctatatct ttaatcgtta tgagtaaagc tcataatgtt
     2641 ggtaatgtca gtcttgaaca gtatttattt ggttctatta tcactattgg gaaggaacaa
     2701 gtgattgctt tgtttgttat tgcattaatc acttttattt taaccatcct atttattaga
     2761 ccaatgtata tattaatgtt tgatgaggat acagcttttg tagatggatt gcctgtgcgt
     2821 acaatgtcta ttttgttcaa tgttgttaca ggtattgcta ttgcgttaac aattcctgcc
     2881 gcaggtgctc tattggtgtc aactattatg gttttaccag ctagtattgc tatgcggtta
     2941 ggtcgaaatt ttaaaactgt tatttttctt gggatgttaa ttggatttgt gggaatggta
     3001 gctggtattt tcctttctta ttattgggag acacctgcta gtgcaacaat aacgatgatt
     3061 tttattggca ttttcctatt agttagctta gtaggcttgc ttcgcaaacg ttaaataaaa
     3121 aaatctatct tattctagga tagatttttt atttatctcc ctgttttttt cgaatatatt
     3181 ttatgaggga tcagttgaga aattatctcg atgaggtatc tactaagttt tctggcttaa
     3241 gttgatagaa ggggcaatgt gaatctattt ttgcgtttta ggataaaaaa tactaccggc
     3301 aattttgccg gcagtaaatt gtaattattg tgcttcagta gtgctgtgat atttgtgttg
     3361 atttaacgct tcttgttcag tcatttgaac gatattagcg gtatttgcat tttttatgtt
     3421 ttctaaacta taccaataag tatttgaacg acccttattt gcaacgtaga cgattctatt
     3481 tttgggagct acattatttg tatctgattc agaagtatta gtgttagcat tttcttctat
     3541 atcagatgca ccttcaatag ctgcttcgtt ttcatcagca tcgctatttg gatcttcacc
     3601 ttcttctgcc tgtttcccat cttctttgat tagcatacca gttttgtaat caatttttgc
     3661 gagtggagac gtattttcta atgtaacagt tgtgacgccg tattcatcaa cttgttcttt
     3721 gccgccgcct aatttgattt ctagaagttt tccttgttta tcaattccaa catactgaag
     3781 ttcaactcgg ctaggtacta aattatttcc ttcgtatatt ggagtcactt tgtaatcaag
     3841 ctagaaattt ttatgatttg ccaaccaact atctaatcta ttttcgtagt ataacatacc
     3901 atcaggatta ttttcgctaa atcctgtatt tagatatttg gtcatagtaa ctaggttctc
     3961 aggaacattg ttcattcctg aaaattgata accaacgagg tgacctcggt ctataagcca
     4021 agttgtttta cccttttcag tagtaaattt gtagttgtgc caaccggttg gattatacgt
     4081 cagtctttct ctttttatct taggctcatc tgcatccctt aattgtatgt gagaatatgt
     4141 tgctctttct aagttatcta atgaacctac cttgatttgc ttattatgag taaacggtaa
     4201 gatttttttg aagaagtact tacatactgt gtctcaacat gggatgtcgt cttataatgt
     4261 ttagccttag cttcaacatt ctgttgtaat gccccaacgc atagtacgac agttaatagt
     4321 gggagtgatt tgtataattg tttagataat ttcataaacc taaacccttc taaatgatat
     4381 ttgtgaaggc ctttcacaag tataatttta tcattttatt gggaaaaagt aataaaaaaa
     4441 cataactagt taattttttt ggagattatg ttatttaact agatagttat t
//
LOCUS       DATROQ010000121         4441 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.122, whole genome shotgun sequence.
ACCESSION   DATROQ010000121 DATROQ010000000
VERSION     DATROQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.122"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..668
                     /locus_tag="VBC94_001878"
     CDS             <1..668
                     /locus_tag="VBC94_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar synthetase-like protein"
                     /protein_id="HEO6980115.1"
                     /translation="FYLMDRTIAYYNEADVLIAPSQAMVDKLQSYGLTVKKILVQGMW
                     DHPTNITLQAVNHKKLVHFPGNPERFNFIKNWRIPTELHVYTDHNMQLPTTVVKEPYQ
                     SDEQLIMKMSEGGYGLVWMDDRDKQYQSLYCPYKLGAYIAAGIPVIIQKGIANQDIIE
                     KNNLGFIIEKIDDISNIVESTTEEEYMEIVSDVRRFNPLVRQGYFTRKLLTDAVFSAL
                     NSM"
     gene            676..2388
                     /locus_tag="VBC94_001879"
     CDS             676..2388
                     /locus_tag="VBC94_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEO6980116.1"
                     /translation="MKELEEIVVFPINKSLDYILENHSSVIRFGDGEFDIIMGKSIPY
                     QDYNPELADRLLQLLHKQSTNDLLVCLPDMFQNRQRYNQSAQLFWKNHFQKFGNFYRQ
                     HCYQDWYGSSFISRPYMDLQDKESAVESFKKLKLLWDKRDILVVEGETSRSGVGNDLF
                     NNCNSLKRIICPSKNAYIKYDEILEAVERYAQGRIILLMLGPTAKILVRDLSDRGFQA
                     IDIGHIVSEYEWYKMGATQKIKFSHKHTAEFNDDENINLIDSQNYIDEIVKTITIDDT
                     EFKDTELISIILPVYNMSGSLSRCLDSILRQVYSTFEVILVNHGSTDGSGNICEKYAM
                     KDERIRLFHEEHQVITSVWNFALEKSKGKYVTFVNPDDFLDESYLNYLYNKLRANKSD
                     ISATTYTFFNEEEKCFVYFATDDNYFEKTFFAQEWLEQEHSYHDNLKQIFPLLSMKLF
                     RKSLFNNITFPLEDLTGEYTLYNVYLLSRHITFSHIGLYITSQVFSHTEIKRFTGHDI
                     EKMLSRKEERLALLTMVGFNSEKYIKNYKETLLNYQSESLNIGDINLYNKIKQKIALI
                     DYCY"
     gene            2512..3861
                     /gene="gorA"
                     /locus_tag="VBC94_001880"
     CDS             2512..3861
                     /gene="gorA"
                     /locus_tag="VBC94_001880"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEO6980117.1"
                     /translation="MSKQYDYIVIGGGSAGSGTANRAAMYGAKVLLIEGGQVGGTCVN
                     LGCVPKKIMWYGAQVSETLHKYSSGYGFEANNLSFDFTTLKANRDAYVQRSRQSYAAN
                     FERNGVEKIDGFARFIDNHTIEVNGQQYKAPHITIATGGHPLYPDIIGSELGETSDDF
                     FGWETLPNSILIVGAGYIAAELAGVVNELGVETHLAFRKDHILRGFDDMVTSEVMAEM
                     EKSGISLHANHVPKSLKRDEGGKLIFEAENGKTLVVDRVIWAIGRGPNVDMGLENTDI
                     VLNDKDYIKTDEFENTSVDGVYAIGDVNGKIALTPVAIAAGRRLSERLFNHKDNEKLD
                     YHNVPSVIFTHPVIGTVGLSEAAAIEQFGKDNIKVYTSTFTSMYTAVTSNRQAVKMKL
                     ITLGKEEKVIGLHGVGYGIDEMIQGFSVAIKMGATKADFDDTVAIHPTGSEEFVTMR"
     gene            complement(3904..4356)
                     /locus_tag="VBC94_001881"
     CDS             complement(3904..4356)
                     /locus_tag="VBC94_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980118.1"
                     /translation="MSKGYSRQQKKSTKTNTDSTQSPAKHIKTGFSALQKTIALVGSV
                     LSIIVASITITNAINNHKDSSKTDSSTSKTVIIRESGQNSSNQVSTEKDETRASSSQT
                     LENSSASSTPSSSSNSAANNDQTSSSTNNDQTNNTTTDTSTIENTTTP"
BASE COUNT         1478 a          625 c          881 g         1457 t
ORIGIN      
        1 atttttattt gatggataga actatagctt attataatga agcagatgtt ttaatagccc
       61 ctagtcaagc aatggtcgat aagcttcaaa gttatggctt gacagtaaaa aaaatcttag
      121 ttcaaggaat gtgggatcat cccaccaata ttactttaca agcagttaat cataagaagc
      181 ttgttcattt tcctgggaac ccagagcggt ttaacttcat aaagaattgg agaattccta
      241 ccgagttaca tgtttatact gaccataaca tgcaattacc caccactgtg gttaaagagc
      301 catatcaatc ggacgaacaa ttaattatga aaatgagcga agggggatat ggcttagtct
      361 ggatggatga ccgtgataaa caatatcaat ctctttattg cccttacaaa ctgggcgctt
      421 atatagcagc aggaattcca gtgattattc aaaaaggtat tgctaatcaa gatataatag
      481 aaaaaaataa tttgggattt attattgaaa aaattgatga tattagtaat atagttgaaa
      541 gtactactga agaggaatat atggaaattg ttagcgatgt caggcggttt aatcctttag
      601 ttagacaagg atattttacg cgaaaactat taaccgatgc agtatttagt gcattaaata
      661 gcatgtgagg ggaatatgaa agaactagaa gaaatcgtag ttttccctat aaacaaaagt
      721 ttggattaca ttttggaaaa tcattcgtca gttattcgct ttggggacgg tgaatttgac
      781 attataatgg gaaaatcgat tccttatcaa gattacaacc cagagttagc agataggtta
      841 ttgcaacttc ttcataagca aagtacgaat gatctattag tatgtcttcc agatatgttt
      901 caaaatcgac aaagatataa tcaatcggca caactttttt ggaaaaatca ttttcaaaaa
      961 tttggcaatt tctataggca acattgttat caagattggt atggttcttc ttttatttca
     1021 aggccatata tggacttaca agataaggag tctgctgtag aatcatttaa aaaattaaag
     1081 ttactttggg acaaacgcga tattttggta gttgaagggg aaacttcgcg gtcgggtgta
     1141 ggtaatgatc tttttaataa ttgcaatagt ttaaaaagga ttatttgtcc ttctaaaaat
     1201 gcttatataa aatatgatga aattcttgag gctgttgaaa gatatgctca agggaggata
     1261 atcttattga tgctaggtcc gactgcaaaa attttagtaa gagatttatc tgatagagga
     1321 tttcaagcca ttgatatcgg tcatattgtt tctgaatatg aatggtataa gatgggagct
     1381 actcaaaaaa taaaattttc tcataaacac accgctgagt ttaacgatga tgaaaatatt
     1441 aatttaattg acagtcagaa ttatattgat gagattgtta aaacaataac tatcgatgat
     1501 actgaattca aggatactga attaatatca atcatacttc ctgtctataa tatgtctggt
     1561 tctttatcaa ggtgtttaga ttctatcctt aggcaagttt actcaacttt tgaggtgatc
     1621 ttagtcaacc atggttctac agatggaagt ggtaatattt gcgagaaata tgcgatgaag
     1681 gatgagcgta ttcgtttatt tcatgaagaa catcaagtta ttacatctgt atggaacttc
     1741 gcattagaga aatctaaagg gaagtatgtt acttttgtga atccagatga ttttcttgat
     1801 gagtcttact taaattatct ttacaataaa ttaagagcta ataaatctga tatctcagca
     1861 accacctata ctttttttaa tgaagaggaa aaatgctttg tttattttgc aacagatgat
     1921 aattattttg agaaaacatt ctttgctcaa gaatggttag agcaagaaca tagttaccac
     1981 gataatttaa aacaaatatt tcctctcctt tctatgaagc tctttagaaa atcacttttt
     2041 aacaatatca cttttccttt agaagattta acaggagaat acacactgta taatgtttat
     2101 ttgctatcaa ggcacattac attttctcat attggtttat acataacctc tcaagttttc
     2161 tctcatactg aaataaaaag attcacggga catgatatag agaagatgct atctagaaag
     2221 gaagagaggt tagccctgtt aacaatggta gggtttaatt ctgagaaata catcaaaaat
     2281 tataaggaaa ctttattaaa ttatcaaagt gagtcgttga atataggtga tattaattta
     2341 tataataaaa ttaagcaaaa gattgcattg atagattatt gttactagaa ataattttaa
     2401 cagcaagtta ggatatgata atcggtaata taaattgttt acgtagcaga aaattgtgac
     2461 tgtaaacact ttgtgataga gtataggtat caatgattca aggagagagt aatgtcaaaa
     2521 caatatgatt atatcgttat tggtggaggt agtgcaggca gtggtaccgc taatagggca
     2581 gccatgtatg gagcaaaagt cctgttaatt gaaggtggac aagtaggtgg aacttgtgtt
     2641 aacttaggtt gtgtacctaa gaaaatcatg tggtatggtg cacaagtttc tgagacactc
     2701 cataagtata gttcaggtta tggttttgaa gccaataatc ttagttttga ttttactact
     2761 ctaaaagcta atcgcgatgc ttacgtgcag cggtctagac agtcgtatgc cgctaatttt
     2821 gagcgtaatg gggtcgaaaa gattgatgga tttgctcgtt ttattgataa ccatactatt
     2881 gaagtgaatg gtcagcaata taaagctcct cacattacta ttgcaacagg tggacaccct
     2941 ctttaccctg atattattgg aagtgaactt ggtgagactt ctgatgattt ttttggatgg
     3001 gagaccttac caaattctat attgattgtt ggggcgggct atatcgcggc agaacttgct
     3061 ggagtggtta atgaattagg cgttgaaacc catcttgcat ttagaaaaga ccatattcta
     3121 cgcggatttg atgacatggt aacaagtgag gttatggctg aaatggagaa atcaggtatc
     3181 tctttacatg ctaaccatgt acctaaatct cttaaacgcg atgaaggtgg caagttgatt
     3241 tttgaagctg aaaatgggaa aacgcttgtc gttgatcgtg taatatgggc tatcggccgt
     3301 ggaccaaatg tagacatggg acttgaaaat accgatattg ttttaaatga taaagattat
     3361 atcaaaacag atgaatttga gaatacttct gtagatggcg tgtatgctat tggagatgtt
     3421 aatgggaaaa ttgccttgac accggtagca attgcagcag gtcgtcgctt atcagaaaga
     3481 ctttttaatc ataaagataa cgaaaaatta gattaccata atgtaccttc agttattttt
     3541 actcaccctg taattgggac ggtaggactt tcagaagcag cagctatcga gcaatttgga
     3601 aaagataata tcaaagtcta tacatcaact tttacctcta tgtatacggc tgttaccagt
     3661 aatcgccaag cagttaagat gaagctcata accctaggaa aagaggaaaa agttattggg
     3721 cttcatggtg ttggttatgg tattgatgaa atgattcaag gtttttcagt tgctatcaaa
     3781 atgggggcta ctaaagcaga ctttgatgat actgttgcta ttcacccaac tggatctgag
     3841 gaatttgtta caatgcgcta acgctatcaa agaatacaaa aaaagctagg gaacctagct
     3901 tttttaaggt gttgttgtat tttcaatagt tgaggtatcg gtagtagtat tatttgtttg
     3961 atcattgtta gtactagatg atgtttgatc gttattggca gcagaatttg aactgctact
     4021 cggagtacta ctagcagagg aattttctag tgtttggctg gatgatgccc ttgtctcatc
     4081 tttttcagta gaaacttggt tactagaatt ttgtccactt tctcttataa tgacggtttt
     4141 tgaagtagaa gaatcagtct tactagagtc tttgtgatta ttaatcgcat tagtaatagt
     4201 aatagaggca acgataatac ttaaaacact accgactaat gcaattgttt tttgaagtgc
     4261 agaaaaacct gtttttatat gcttagcggg tgattgagtt gaatcagtat tagtcttagt
     4321 agactttttc tgttgtcttg aatatccttt tgacatgacg ccctccttaa atgtacatca
     4381 actattatat gattttatcc taaaaaaagc taaagaaaaa ataatttaaa acatttaaat
     4441 a
//
LOCUS       DATROQ010000122         4324 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.123, whole genome shotgun sequence.
ACCESSION   DATROQ010000122 DATROQ010000000
VERSION     DATROQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.123"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            66..2516
                     /locus_tag="VBC94_001882"
     CDS             66..2516
                     /locus_tag="VBC94_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6980119.1"
                     /translation="MVFMANKKKTKGKKTRRPTKAEIERQRAIQRMITALVLTIILFF
                     GIIRLGIFGITVYNVIRFMVGSLAYLFIAATLIYLYFFKWLRKKDSLVAGFLIASLGL
                     LIEWHAYLFSMPILKDKEILRSTARLIVSDLMQFKITVFAGGGMLGALIYKPIAFLFS
                     NIGAYMIGVLFIILGLFLMSSLEVYDIVEFIRAFKNKVAEKHEQNKKERFAKREMKKA
                     IAEQERIERQKAEEEAYLASVNVDPETGEILEDQAEDNLDDALPPEVSETSTPVFEPE
                     ILAYETSPQNDPLPVEPTIYLEDYDSPIPNMRENDEEMVYDLDDDVDDSDIENVDFTP
                     KTTLVYKLPTIDLFAPDKPKNQSKEKDLVRKNIRVLEETFRSFGIDVKVERAEIGPSV
                     TKYEIKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSF
                     RELWEQSDANPENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISS
                     ILMKARPDQVKFMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYE
                     LFSKIGVRNIAGYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIR
                     LGQKARAAGIHMILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKL
                     LGRGDMLFKPIDENHPVRLQGSFISDDDVERIVGFIKDQAEADYDDAFDPGEVSETDN
                     GSGGGGGVPESDPLFEEAKGLVLETQKASASMIQRRLSVGFNRATRLMEELEAAGVIG
                     PAEGTKPRKVLMTPTPSE"
     gene            2598..4313
                     /gene="pabB"
                     /locus_tag="VBC94_001883"
     CDS             2598..4313
                     /gene="pabB"
                     /locus_tag="VBC94_001883"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEO6980120.1"
                     /translation="MHIETVIDFKELGKRYRFKNPTKELIADTLEQVLEVIKEVDYYQ
                     SQNYYVVGYLSYEASAAFDSHFKVSQQKLAGEHLAYFTVHKDCENEAFPLSYENVRLA
                     DNWTANVSEQEYQEAIANIKGQIRQGNTYQVNYTLELSQQLCSDPFSVYERLMVEQGA
                     GYNAYIAYDDKRILSVSPELFFKKKDEVLTTRPMKGTSARKPTYQEDVAERDWLANDP
                     KNRSENMMIVDLLRNDMGRICDVGTVKVKKLCQVEQYATVWQMTSTIEGVLSPEVTLM
                     SIFQALYPCGSITGAPKISTMAIINELEKRPRGIYCGTIGLCMPDGQAIFNVPIRTVQ
                     MKGQQAYYGVGGGITWESQTDSEYEETRQKSAVLTRVNPKFQLITTGRVTENKLLFSQ
                     QHVERLVESASYFAYSFDKSKFERELKKYLHQLDEKDYRLKIMLDKTGKVTFEVKQLV
                     NLSKKFLTAEVVVQDYPIKLSPFTYFKTSYRPHIIEGQNEKIFVSPEGLLLETSIGNI
                     VLEKNGRFLTPDLSEGGLNGIYRRHLLKNQKVIEAPLTLKDLESADAIYACNAVRGLY
                     PLNLK"
BASE COUNT         1435 a          660 c          913 g         1316 t
ORIGIN      
        1 attatgatag aatagtatag catgagttga tagagaaagt gaggaacaag tatttcactt
       61 gaaatatggt atttatggca aataagaaaa aaacaaaagg aaagaaaacc agaagaccta
      121 ctaaggcaga aatagagcgt caaagagcta ttcaaaggat gattactgct cttgttttaa
      181 caattattct cttctttggt attatcagat taggtatttt tggtattaca gtctataacg
      241 tcatccgttt tatggtaggt agcttggctt acttatttat tgcggcaact ttaatctacc
      301 tttatttctt taaatggttg cgaaagaaag atagcttagt agcaggtttt ttgatagctt
      361 ctttaggatt attgattgag tggcatgctt accttttctc aatgcctatt ttgaaagata
      421 aagaaatttt gcgttcaact gctcgattaa ttgtgtctga tttaatgcaa tttaaaatca
      481 ctgtttttgc cggtggaggt atgttgggtg ctttgattta caagccaatt gcttttctct
      541 tttctaatat tggtgcctat atgattggtg ttctcttcat cattttgggt ctctttttaa
      601 tgagttctct ggaagtttat gacatcgtcg aatttattag agcttttaaa aataaagtgg
      661 cagagaagca cgagcaaaat aaaaaggagc gttttgctaa gcgagagatg aaaaaagcaa
      721 tcgctgaaca agagcgcata gagcgtcaaa aagctgaaga agaagcttat ttagcttcgg
      781 ttaatgtaga ccctgaaacg ggtgagattc tagaggatca agctgaggac aatttggatg
      841 atgcgctacc acctgaggta agtgaaacat caactccggt atttgagcca gagatccttg
      901 cttatgagac atcgcctcaa aatgatcctt taccagtaga gccgacaatt tatttagaag
      961 actatgattc gccgattcct aatatgagag aaaatgatga ggaaatggtt tatgatttag
     1021 atgatgatgt agatgatagt gatatagaaa atgtcgactt tacacctaaa acgacactgg
     1081 tttataaatt accaacgata gatttatttg caccagataa gcctaaaaat caatccaaag
     1141 aaaaggattt agtccgaaag aatatcagag ttttagaaga aacatttaga agttttggta
     1201 tcgatgtaaa agtagaacgt gctgaaattg gaccatcagt tactaaatat gaaattaaac
     1261 cagcagttgg agttcgtgtg aatcgtattt caaatctatc tgacgaccta gctcttgctc
     1321 ttgcagcaaa agatgtgcgt atagaagcac caattcctgg aaaatcatta ataggtattg
     1381 aagttcctaa ctcagaaatt gcaacggttt ctttccgcga actttgggaa caatctgatg
     1441 ccaatcctga aaacctttta gaagtaccac taggaaaagc tgttaacggc aatgctcgca
     1501 gttttaactt agctagaatg ccgcatcttt tggtagctgg ttcaactggt tcaggtaaat
     1561 ctgtggcagt taatggaatt atttcaagta ttttgatgaa ggcacgtcca gatcaagtta
     1621 agtttatgat gattgatccc aaaatggttg aattatctgt ttataatgat attccacatt
     1681 tattaatccc tgttgtaacc aatccgcgta aagcaagtaa ggcactccaa aaagttgttg
     1741 atgaaatgga aaatcgatac gagttattta gcaaaattgg tgtgcgtaat atagcaggtt
     1801 ataatacaaa ggttgaagag tttaatgctt cctctgagca aaaacaaata cctttgcctt
     1861 taatcgttgt cattgtagat gaattggctg acttgatgat ggttgctagt aaagaagttg
     1921 aagatgctat tattcgtttg gggcaaaaag cacgtgctgc aggtatccat atgattcttg
     1981 caactcaacg tccatccgta gatgttattt ctggtttgat taaagcaaat gttccgtcgc
     2041 gtattgcatt tgctgtttca agtggtactg atagccgtac gatccttgat gaaaatggtg
     2101 ctgaaaagct cttgggacgg ggtgacatgc tctttaagcc tattgatgag aatcatccag
     2161 tacgactaca aggttccttt atttcagatg atgatgttga aaggatcgtt ggttttatca
     2221 aagaccaagc cgaggctgac tatgatgatg cctttgatcc tggagaagta tctgaaacag
     2281 ataacggctc tggtggtggc ggcggagtac ctgaaagtga tcctcttttt gaagaagcca
     2341 agggactcgt tttagagacg caaaaagcaa gtgcctcaat gattcaacgc cgattgtctg
     2401 ttggtttcaa tagagcaaca agactaatgg aagaattaga agcagcgggg gttattggtc
     2461 cagcagaagg aaccaagcca cgaaaagttt taatgactcc aactccgagt gaataagaaa
     2521 aaactaatct gacaagaaat tgtcggatta gttttttatg ttataatgaa gcaaatagat
     2581 gaagaagaag gttacgaatg catattgaga ctgttattga tttcaaagaa ttaggaaaaa
     2641 gatatcgttt taaaaatcct acaaaagaat taatagctga tactttagaa caagtcttag
     2701 aagtgataaa agaagttgat tattatcaat ctcaaaatta ttatgttgtt ggttatttat
     2761 cttatgaagc atctgctgct tttgattcac attttaaagt ttctcaacag aagttggctg
     2821 gagaacatct agcttatttt acagtacata aagattgtga gaacgaagct tttcctttaa
     2881 gttatgaaaa tgttagatta gcagataatt ggactgctaa tgtttctgag caagaatatc
     2941 aagaggcaat tgctaatatt aaaggacaaa ttagacaagg gaatacttat caagtaaatt
     3001 atacactaga gcttagccaa caattatgct cggatccctt tagtgtttat gaacgcctaa
     3061 tggtagaaca aggagcagga tataatgctt atattgccta cgacgataaa aggattttat
     3121 ctgtaagccc ggaattgttc tttaagaaga aggatgaagt cttaacaact agaccaatga
     3181 agggaacaag cgctagaaag cctacttatc aagaagatgt ggcggagcga gattggttgg
     3241 caaatgatcc taaaaatcgt tctgaaaata tgatgattgt tgatttatta agaaacgata
     3301 tgggccgtat ctgtgatgtt ggaacagtta aggtaaaaaa actatgtcaa gtggagcagt
     3361 atgcaactgt gtggcaaatg acatcaacta ttgaaggagt tttatcgcca gaagtgacac
     3421 ttatgtctat ttttcaagct ctatatcctt gtggatctat cactggagct cctaagatat
     3481 caacaatggc tattattaat gagttggaaa aacggccaag aggtatttat tgtgggacga
     3541 taggactttg catgccagac ggacaagcta tttttaacgt tcctattcgc acagtacaaa
     3601 tgaaaggtca acaagcctat tacggcgttg gtggaggtat tacgtgggag agccagacag
     3661 attctgaata tgaagaaacc cgtcaaaaat cagctgtttt aacacgtgtc aatccaaaat
     3721 ttcaattaat aactacagga agagtgactg aaaataaact tcttttctct caacaacatg
     3781 tcgagagatt agttgaatca gcgtcttatt ttgcttattc ttttgataaa agtaaattcg
     3841 aaagggaact gaaaaaatac cttcatcagc tagatgagaa ggattaccgc ttgaaaataa
     3901 tgcttgataa aactggtaag gtaacgtttg aggtaaaaca actagtgaat ttatcaaaaa
     3961 agtttttaac ggcagaggtg gtcgtacaag actaccctat taaattatcc ccgtttactt
     4021 attttaaaac ttcttatcgc ccacatatta ttgaaggtca gaatgaaaag atatttgtat
     4081 ctcctgaggg gttgctattg gaaacaagta ttgggaatat tgttttagaa aaaaatggaa
     4141 ggtttttaac cccagattta tcagaaggag ggttgaatgg gatttatcgt cgtcatctcc
     4201 ttaaaaacca aaaagtaatt gaagcaccac taactttaaa agatttagaa tcagccgatg
     4261 ctatatacgc ctgtaatgct gttagagggc tttatcctct aaacctaaag taaaaagagt
     4321 tgga
//
LOCUS       DATROQ010000123         4309 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.124, whole genome shotgun sequence.
ACCESSION   DATROQ010000123 DATROQ010000000
VERSION     DATROQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.124"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            121..1860
                     /locus_tag="VBC94_001884"
     CDS             121..1860
                     /locus_tag="VBC94_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6980121.1"
                     /translation="MTLFLIMMERAGLIILLAYAFVHIPFIKQTLKQPELKKHQYILL
                     ILFSLFAIISNFTGVEIQSDLSIIPQTLNHIADQSSVANTRVLTIGVSGLIGGPIVGI
                     IVGLLSVFVRYLQGGLAPHIYVISSLLIGLCSGLSGNYLRKNYNKIRVLDAMVVGFGM
                     EILQMICILIFSVDFNQALRLVSFISMPMILSNTLGLGIFISIISSTQKLEEHAKAFQ
                     THQVLELANLTLPYLRKGLTTESCQPVAEIIHKHMDVSAVSLTSQSAILAYVGDGADH
                     HLPNTQILTKLAKRAIDTGKVSVATDKSEIECDHRNCPLSSAIVIPLHIHDVIVGTLK
                     LYFSDAQHMTYVDRQLAEGLGNIFSTQLALGQAEEATRLLQDAEMKSLQAQVNPHFLF
                     NALNTIYGLIRMDSEKARKLVQDFSKVIRANLQRAKQNLIPLHDELEQVNAYLALEEA
                     RFPNMVAFNLDNQTNSDDNLMIPPFTLQVLIENSYKHAFKHINKNNQLKVTIARNNDR
                     LHIIVQDNGIGIPKEKLITLGKKTQISKKGSGTAIENLVRRLNIIYDGQASLKFESND
                     SGTCAIVNIPIKN"
     gene            1872..2606
                     /gene="ltdR"
                     /locus_tag="VBC94_001885"
     CDS             1872..2606
                     /gene="ltdR"
                     /locus_tag="VBC94_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LtdR"
                     /protein_id="HEO6980122.1"
                     /translation="MKVLVVDDEPVARNELIYLLNKYDSNLVIAEAHDMATALAILLR
                     ETFDVALLDIHLRDDSGLQLAEYINKMPKPPLLIFATAYDQYAIQAFEQDARDYLLKP
                     YEFDRLKQAMDRVKGALSTSTIIESVASGPLFKQQYPLTVEDRIYLVSADDILLIEAM
                     QGKLIIQTPDKNYEIDGSLQQWQDKLPSSQFVRVHRSYIVNINAIKTIEPWFNQTLQL
                     HLCNKITVPVSRANVKPLKQMLGIST"
     gene            2762..4246
                     /locus_tag="VBC94_001886"
     CDS             2762..4246
                     /locus_tag="VBC94_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEO6980123.1"
                     /translation="MVTFLGGVALLIVGYFTYGRYIEKNFQIDENRQTPAEALRDGYD
                     FVPMPKWKNGMIELLNIAGTGPIFGPILGALYGPVAYIWIVLGCIFAGAVHDYMIGMI
                     SLRNNGAYLPELASRYLGKSMKHVINIFSMLLLILVATVFVVTPANLILSILPAGTLS
                     LPWIIGLIFVYYLISTVLPIDKALGKVYPVFCVILMVSTAAVGFRLLTGGFDMPNLTF
                     ETFKNMHPAGLGIFPALFFTISCGAISGFHATQAPMVSRTTVNEREGRFTFYGMMIAE
                     GVIAMIWAGASMSLFKGQNLYEMIAAGTPSAVVNQVMLMLLGSVIGTIAIIGVIVLPV
                     SSGLSAFRSLRTIVADYIHVKQDTLPKIFAVTIPLYVISFVLTHVDFNLLWRYFNWAN
                     QVTAVIGLLVATRYLILKRRNYWVTFVPAMFMLYAVVVYILSQPIGFNMGLGILTYSL
                     ALVLTGILVGLFWKSGQKQLKTVHPEAFLFNDHRPINYYSSLDS"
BASE COUNT         1350 a          745 c          761 g         1453 t
ORIGIN      
        1 gatggatatc cccttaattg tcttgagaat acttgataga gagacatttt aaatgagtaa
       61 tgaaatgaat cattttatac ctatgttata acggactaat taatactttg gagagattta
      121 atgacactct ttttaattat gatggagcga gcggggttaa tcatattatt agcctatgcc
      181 tttgtgcata ttccctttat aaaacaaact ttgaagcaac ccgaattaaa aaaacaccag
      241 tatattctgc ttatcttatt tagcttattt gctattattt ctaattttac aggggtagaa
      301 attcagtcag atttatctat cattccacaa actcttaatc atatagcaga tcaatcatca
      361 gttgccaaca cacgtgtttt aaccattggt gtatcaggtc taatcggtgg ccccatagtc
      421 ggtattatag ttggtctctt atctgtattc gttaggtact tgcaaggagg attagcccct
      481 catatttatg tcatttcttc cttactaatt ggtttatgct ctgggttatc aggcaattat
      541 cttcgtaaaa attataataa aattcgtgtt ttagatgcaa tggttgttgg tttcgggatg
      601 gaaattctac aaatgatctg tatcctcatt tttagtgttg actttaatca ggcgcttcga
      661 ttagtatctt ttatcagcat gccaatgatt ttaagtaata ctttaggttt aggtatattt
      721 atatccatta tttcatctac tcaaaaatta gaagaacatg ccaaagcatt tcaaacccat
      781 caagtacttg aattagcaaa tttaaccctt ccttacctta gaaagggact gacaacagaa
      841 tcttgtcaac cagtagctga aattattcat aaacatatgg atgtgagtgc tgttagcctg
      901 actagtcaat cagccatttt agcctatgta ggagatggag ctgaccacca tcttcctaat
      961 actcaaatat taacaaagct tgctaaaaga gcaatagata ctggtaaagt tagtgtcgca
     1021 actgataaaa gtgaaatcga atgcgaccat aggaattgcc cacttagttc agcaattgtt
     1081 attccattac acatccacga tgttatagtg ggtactttga aattatattt ttcagacgca
     1141 caacatatga cttacgttga ccgtcaatta gctgaaggat taggcaatat cttttccact
     1201 cagttagctt tgggacaagc tgaggaagca acaaggttac tacaggatgc ggaaatgaag
     1261 tctctccaag cgcaggttaa tccgcatttt ctttttaatg cactcaatac tatttatggt
     1321 ttaattcgca tggatagcga aaaagcaaga aagctcgtcc aagattttag caaggttatt
     1381 cgtgctaatc ttcaacgggc aaagcaaaat ttaattcctc tccatgatga attggaacag
     1441 gttaacgcct atttagccct tgaagaagca agatttccta acatggtggc ctttaattta
     1501 gacaaccaaa caaattcaga tgacaactta atgattcctc cctttacatt gcaggtcttg
     1561 attgaaaact cttataaaca cgcttttaag catatcaata aaaataacca gctaaaggtt
     1621 actattgcaa gaaataatga taggcttcat atcattgtcc aggacaatgg tattgggatt
     1681 ccaaaagaaa aattaataac gcttggaaaa aaaactcaaa tttcaaaaaa aggttctggt
     1741 acagctattg agaatctcgt tcgtcgcttg aatattattt atgatggaca agcaagttta
     1801 aaatttgaat caaatgattc aggaacttgt gctattgtta acatccccat taaaaattag
     1861 aaaggtattg tatgaaagtt ttagtagttg atgatgaacc agttgcacgt aacgaattaa
     1921 tttaccttct taataagtat gattctaacc tcgttatagc agaggcgcat gatatggcta
     1981 ctgcattagc tattttactt agagaaactt ttgatgtagc actgttagat atccatctca
     2041 gagatgattc tgggttgcaa ttagcagagt atatcaataa aatgcccaaa ccaccattat
     2101 tgatattcgc gactgcttat gatcaatatg ctattcaggc ttttgagcag gatgcgcgtg
     2161 attatttgtt aaaaccctat gagtttgata ggttaaagca agctatggat agagtaaaag
     2221 gagcgctaag tacatctaca attatagaga gcgtagcttc cggtcctctc ttcaagcaac
     2281 agtatccatt gacagtagaa gatcgaatct atctggtgtc ggcggatgat atccttttga
     2341 ttgaagctat gcaaggaaaa ctgattatac aaacacctga taaaaattat gaaattgatg
     2401 gctctctaca acaatggcaa gataaactac catcatctca atttgtacgg gtacatcgct
     2461 cttacattgt gaatattaat gctattaaaa cgattgaacc ttggtttaac caaacacttc
     2521 agttacacct ttgtaataaa ataacagttc ctgttagcag agcaaatgta aaacccctaa
     2581 aacaaatgtt aggcatatct acctaactaa tgaaaagtta ttcatcataa aacttagact
     2641 attcatttct aaaaaacaac cattcatctt gaaaatagga aatttcaatt tttcaatgct
     2701 atactaaagt catgaaaacg cttaataaaa ttaagcaaaa aaatttttag gaggcagtga
     2761 tatggtaaca tttttaggag gggtagccct tttaattgtt ggttatttca cgtacggtag
     2821 atacattgaa aagaattttc aaattgatga aaatcgccaa acacctgcag aagcattaag
     2881 ggatggatat gattttgttc ctatgccaaa atggaagaat gggatgatcg agttattgaa
     2941 catcgcagga actggaccta tctttggacc tattttagga gcactctatg gtccagttgc
     3001 ttatatctgg atagtcttag gatgtatttt tgcaggggct gttcatgatt atatgattgg
     3061 aatgatttct cttagaaata atggtgcata tcttcctgaa ttagctagtc gctatcttgg
     3121 aaaatcaatg aagcatgtga tcaacatttt ttcaatgcta ttacttattt tagttgcaac
     3181 cgtatttgtg gtaactccag ctaatcttat cctgagtatt ttaccagctg ggacactatc
     3241 tttaccttgg ataatcggtc ttattttcgt ttattacctt atttctacag tattacctat
     3301 tgataaagca cttggtaaag tttatcctgt attctgcgtc atcctaatgg ttagtacagc
     3361 agcagttggc ttccgtttat taactggtgg ctttgatatg ccaaacttaa cttttgaaac
     3421 ctttaaaaat atgcatccag ctggacttgg gatattccct gctttattct ttaccatttc
     3481 atgtggagcc atctctggtt tccatgctac ccaagcacct atggtctcaa gaacaactgt
     3541 taatgagaga gaagggcgtt tcactttcta tggtatgatg attgcagaag gagtaattgc
     3601 gatgatttgg gcaggtgctt caatgagctt gttcaaaggt caaaatctct atgaaatgat
     3661 tgctgctgga acaccttctg cagttgtcaa tcaagtaatg ttaatgttac tcggtagtgt
     3721 aattggaact atcgctatca ttggtgttat tgttctccct gtttcatctg gtttatcagc
     3781 tttccgtagc ttacgtacta tcgttgctga ttatatccat gtcaaacaag atactcttcc
     3841 aaaaatcttt gctgtaacta tcccattata tgttatttca tttgtcctta cacacgttga
     3901 ttttaatctc ctatggcgtt actttaactg ggcaaatcaa gtaactgctg ttattggttt
     3961 attagttgct acaagatatc tcatcctaaa aagacgaaat tattgggtaa catttgtacc
     4021 agctatgttc atgttgtatg cagtagttgt ttatatttta tctcaaccaa ttggatttaa
     4081 tatgggctta ggcatcttaa cttattcact tgctttagtg ttaacaggta ttcttgttgg
     4141 acttttctgg aaatctggtc aaaaacaatt aaaaactgtt catcctgaag cattcctgtt
     4201 taacgatcat agaccaatta attattacag tagtttagat tcttaatatt attttgaagc
     4261 catgttatta agacttacag ttatcacaat aacctcgaat gaagtatca
//
LOCUS       DATROQ010000124         4239 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.125, whole genome shotgun sequence.
ACCESSION   DATROQ010000124 DATROQ010000000
VERSION     DATROQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.125"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(347..799)
                     /locus_tag="VBC94_001887"
     CDS             complement(347..799)
                     /locus_tag="VBC94_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEO6980124.1"
                     /translation="MNRLSWEDYFMANAELISKRSTCDRAFVGAVLVKNNRIIATGYN
                     GGVSETDNCNEVGHYMEDGHCIRTVHAEMNALIQCAKEGISTNNTEIYVTHFPCINCT
                     KALLQAGVKKITYKANYRPHPFAIELMEAKGVAYVQHDVPEVTLGMEE"
     gene            complement(811..1878)
                     /locus_tag="VBC94_001888"
     CDS             complement(811..1878)
                     /locus_tag="VBC94_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO6980125.1"
                     /translation="MGFEQRLTRCQTAISQLSCDALLITNLTNIFYLTGFSGTNATVL
                     ISPKHRIFVTDSRYALIAKNTVREFDIIISREPLAAILKIIRDDALIAIGFETDISYH
                     MYKHMVEVFEDYRLIEAPSVVEKLRMIKDVTELAIIRQACRISDQAFLDVLDFIKPDR
                     TTELQVANFLDFRMRELGATGPSFDFIVASGYRSAMPHGVASQKTIQSGETLTLDFGC
                     YYQHYVSDMTRTIHIGHVTDQEREIYDIVLKSNQAIIDNVKSGMKRCDYDYLARQVIE
                     NSGYGNHFTHGIGHGMGLDVHEIPYFGKSEGVIASGMVVTDEPGIYLDNKYGVRIEDD
                     LLITETGCEVLTSAPKELIVL"
     gene            complement(1978..2694)
                     /locus_tag="VBC94_001889"
     CDS             complement(1978..2694)
                     /locus_tag="VBC94_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEO6980126.1"
                     /translation="MIILIIALATILTFVTWMLIRPKALRYILGLLSLALLFASVWML
                     TDHFINHTGMTIETKTKTREIYSAGDSKAPFGLMIYKEIGTHSSNNVLVYRTDLNQKE
                     AKAHFVPNTKEVTSAVKKSASYQLADVKKATVTTKTKRYVWKDSLSKLLYGFAGENGE
                     LISQKSVAKIPRKTWLVLTKEQSEHLESAAAKLKEQMTSQAKVNPVKAMEIKTLAKTN
                     PEAYAEMQVKALKEALNIKE"
     gene            complement(2696..4195)
                     /locus_tag="VBC94_001890"
     CDS             complement(2696..4195)
                     /locus_tag="VBC94_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO6980127.1"
                     /translation="MTTKAKHDIHGKPYNRTAMITLLLIATFAGVLNQTSLGTAIPTL
                     MNSFNISLSTAQQATTWFLLANGIMIPVSAYLATRFSTKWLYVTSYVVLLIGLLMTTL
                     APTSNWNLFLVGRIIQAISVGISMPLMQVVMVNVFPPEQRGAAMGLNGLVVGLAPAIG
                     PTLAGWILKQEFHFAGHDLTWRAIFLLPLLILTVTTILSPFVLKDVVDNKSVKLEVPS
                     LILSIIGFGSFLWGFTNVATYGWGDIGYVISPIMVGIIFIALFIHRQLKLETPFLDIR
                     VFKNKQFSVTTAAIALSMMAMMGVEMMLPLYLQNVHGLSALDSGLALLPGALMMGIVS
                     PISGAVYDKVGARRMAMIGFTILGVATLPFVFLTTTTPDHFITLLYAVRMFGIAMVMM
                     PLTASAMSALPPHEAAHGTAANNTARQIASAVVVALLSSVAQNIITNNKPSKDLLTMN
                     PLKYANQMLNASLDGFHVSFAIGFVFAVLGLLVSLFLRKGKIIETEKEV"
BASE COUNT         1383 a          866 c          670 g         1320 t
ORIGIN      
        1 catattagca taaactggat ttacatctat tgaggtcagc aacaatatct tttctctttt
       61 ttgtaaatta tttttgcaat tcgagattcc cagcagtact aattttagat gttgataata
      121 gatggatcag ctattctatt atcaacatct aaaactttct gtgaaaaatc attaggaata
      181 atgactatta actggtgatc acctttttta ggccattttc agtaatccca tgactaacaa
      241 ttgaccaatt ttgagaatta tctcgttcaa tatatttaac ataactggcc cttagcttaa
      301 acgataaaac cttcaaggaa gataggcata tactctatct tcctctttac tcttccatcc
      361 ctagtgttac ctctggaaca tcatgttgaa cataagcaac tccttttgct tccataagtt
      421 caatagcaaa tgggtgtggg cgataattag ctttataagt aattttctta accccggctt
      481 gcaagagcgc cttggtgcaa ttgatacaag gaaaatgcgt cacataaatt tctgtattat
      541 tagtagaaat accttcttta gcacactgaa taagggcatt catctcagca tgaacagtcc
      601 taatacagtg accatcttcc atataatgtc ccacttcgtt acagttatct gtctcagaga
      661 ctcctccgtt ataacctgta gcaatgatgc gattattttt gactaaaaca gctccaacaa
      721 aagcacggtc acaggtagaa cgtttagaaa tcaactctgc atttgccata aaataatctt
      781 cccaagaaag tctattcatt ctattttcct ttaaagtaca attaattctt tcggtgcaga
      841 tgtcaatact tcgcaacctg tttccgttat taataaatca tcttcaatac gaacaccgta
      901 tttattatcg agatatattc ctggctcgtc agtcactacc atcccagatg caatcactcc
      961 ctctgactta ccaaaataag gaatttcatg aacatcaagc cccataccat gccctatacc
     1021 gtgggtaaaa tgattaccgt atccagaatt ttcaatgact tgtcgcgcca agtaatcata
     1081 atcacaacgc ttcattcctg attttacatt atcaataata gcttgattac ttttcaaaac
     1141 gatatcgtaa atctcgcgtt cctgatcagt gacatgaccg atatgaatcg tacgtgtcat
     1201 atcactaaca tagtgctgat agtaacaacc aaaatctaaa gtcagagttt ctcccgattg
     1261 aatagtcttc tgactcgcaa ccccatgtgg catcgctgat cgatatccag atgctacaat
     1321 aaaatcaaat gaaggaccag ttgctcctaa ttctctcatg cgaaagtcta aaaaattagc
     1381 aacttgcaat tcagttgttc gatcaggctt tataaaatcc aacacatcta aaaaagcttg
     1441 gtccgaaata cgacaggctt gacggatgat cgcaagttct gttacatctt taatcatacg
     1501 taacttttca acaacagatg gagcttctat caagcgataa tcttcaaaaa cttccaccat
     1561 atgtttgtac atatgataag aaatatcagt ttcaaaacca atcgctatca atgcgtcatc
     1621 gcgaataatt ttcaaaatag ccgcaagtgg ttctctgcta atgataatat caaactctct
     1681 tacagtattc tttgctatca aggcatatcg tgaatctgta acaaaaatcc ggtgttttgg
     1741 actaatgagt accgttgcat tagttcctga aaaaccagtc aaataaaaaa tatttgttaa
     1801 atttgttatt aagagtgcgt cacatgataa ttgtgaaatt gctgtttgac atctagttag
     1861 acgttgttca aaacccataa gaaacctcgc tattctaacc ttaattaggt taattttatc
     1921 atcttttaaa acatttagct ataagaaaag caactcattt cagttgcttt tactttacta
     1981 ttcttttata ttaagagctt cttttaatgc tttaacttgc atctcagcat aagcttctgg
     2041 atttgtctta gctaatgtct ttatctccat agctttcact gggtttactt ttgcttgcga
     2101 agtcatttgt tcttttagtt tagccgcagc cgattctaag tgttcggact gctctttagt
     2161 caaaactagc caagtttttc gtggtatttt agcaactgat ttctgagaaa tcaattctcc
     2221 attttcacct gcaaagccat aaagtagttt cgaaagacta tctttccaca cataacgttt
     2281 agtctttgtt gttactgtgg cttttttcac atcagctaat tggtagctag cagatttttt
     2341 cacagcactt gtcacttctt ttgtatttgg aacaaagtga gcttttgctt ctttttgatt
     2401 taaatctgtt cgataaacta aaacattatt actcgaatga gtaccaatct ctttataaat
     2461 catgagacca aatggagcct tactatcacc cgcactataa atctcacgtg tctttgtctt
     2521 agtttcaata gtcatacctg tatgattaat aaagtgatcc gttaacatcc aaactgaggc
     2581 aaacaacaaa gctaatgaga gaagacctag aatataccgt agagcttttg gacgaatcaa
     2641 catccatgtg acaaatgtca aaatagttgc cagtgcaata atcaaaataa tcatgctata
     2701 cctccttttc cgtctcaata attttccctt ttctcaggaa aagagatacc agtaaaccaa
     2761 gaaccgcaaa tacaaaacca atggcaaaag aaacatggaa gccatcaaga ctagcattca
     2821 acatctgatt tgcatatttt aaaggattca ttgttagcaa atcttttgat ggtttattgt
     2881 tggtgataat attttgagca acacttgaaa gcaatgcaac tacaactgct gaagcaattt
     2941 gacgagcagt attattagct gctgttccat gtgcggcctc atgtggtgga agtgcgctca
     3001 tcgcacttgc tgtcaatggc atcataacca tagcaatacc aaacatacgt actgcatata
     3061 agagggtaat aaaatgatct ggtgttgtag tcgttaagaa aacaaaaggt aacgtcgcta
     3121 cacctagtat ggtaaaacca atcatagcca tacgtcttgc accaacttta tcatatacag
     3181 ctccagaaat tggactaact atccccatca tcaaggcacc tggtaataaa gctaaaccag
     3241 aatcaagtgc agagagacca tgaacatttt gcaagtaaag cggtagcatc atttcaacac
     3301 ccatcatcgc catcattgaa agtgcaatag ctgctgttgt tactgagaat tgtttatttt
     3361 tgaaaacacg gatatctaag aacggtgttt ctagttttaa ttgacgatga atgaataagg
     3421 cgataaaaat aataccaacc ataataggag aaataacata tccaatatct ccccatccat
     3481 aagttgccac atttgtgaag ccccacaaaa agctaccaaa gccaattatt gaaaggataa
     3541 gggaaggcac ttccaattta actgacttat tatcaaccac atcttttaga acaaaggggg
     3601 ataaaattgt agtgaccgtt aaaattagta aaggaagaag gaaaattgca cgccatgtta
     3661 aatcatgccc tgcaaaatga aattcttgtt ttaaaatcca ccctgctaat gtaggcccaa
     3721 tagctggggc aagaccaacc accaaaccat taagtcccat agcggcacca cgctgctctg
     3781 gaggaaaaac attcaccata acaacctgca tcaaaggcat agaaatccct acagaaatcg
     3841 cttgaataat acgtcctact aaaaaaaggt tccagttaga tgtaggtgct aacgtcgtca
     3901 ttaacagtcc aataagtaaa acaacgtagg atgtcacata caaccatttt gttgaaaaac
     3961 gtgtcgctaa ataagctgaa acaggaatca taatcccatt cgctaataaa aaccatgttg
     4021 ttgcttgttg agccgttgac aacgaaatgt taaagctatt catcaaagtt gggatagccg
     4081 ttcccaaact tgtctgattt aatacccctg caaatgtagc aatcaacaat aaggtgatca
     4141 tggctgtacg attatatggt ttgccatgaa tatcatgttt tgcttttgta gtcattttat
     4201 ctccaatctc tatttctgat tttaactaat atcttttaa
//
LOCUS       DATROQ010000125         4148 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.126, whole genome shotgun sequence.
ACCESSION   DATROQ010000125 DATROQ010000000
VERSION     DATROQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.126"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..256)
                     /locus_tag="VBC94_001891"
     CDS             complement(<1..256)
                     /locus_tag="VBC94_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_154412286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6980128.1"
                     /translation="MEKELGKTLRRLRKGKKVSISSLADEHLSKSQISRFERGESEIT
                     CSRLLNILDKLNITIDEFVSIHSKAHTHFFILLNRVRKYCA"
     gene            344..412
                     /gene="shp2"
                     /locus_tag="VBC94_001892"
     CDS             344..412
                     /gene="shp2"
                     /locus_tag="VBC94_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEO6980129.1"
                     /translation="MKKINKALLFTLIMDILIIVGG"
     gene            473..2062
                     /locus_tag="VBC94_001893"
     CDS             473..2062
                     /locus_tag="VBC94_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HEO6980130.1"
                     /translation="MKIKKIISGFAAALIISSLSTINYEVKADDTTSEYHYISKQNNE
                     KQLISYIKEQHRLLNQFVVDNVNSFTQLNANPTIEQLNRAITLFKQKDEQLFNQVKAG
                     HLSPSNYNAIVNQRNVINQTVQNLIDQNHNKIQTSQNKAAQLVGQRNQVVNKIQAILA
                     TVNYNSVNSIQEAENLFHSLRNQIEPLVAEVNNYKAAMAILQQEVDALSTAAIETETS
                     KLATLKVSENTSVPANKVEEKTTQSEASGNKQEVTKSEEKQATSDAKASQPESANIAD
                     YDSLKEVLRNNISNQVPHISVQMEFKTQEQVDEYQKNLGSIIREIGDTLGTATEFNAK
                     SNISTYTLGGQIQRIIVKSDITITYTLKGDMVGLHKEYKQFVDSFVKENITNKNITSD
                     YEKAKVIHDHLVNNYTYATEELATTRETASGISIHAPEALYKDKRGVCQAFAVMFKDM
                     AAAGLSVWYVTGQAGGGNHAWNIVTINGVKYYVDTTWDNNIKSNKYFLVGKTIMDADH
                     LLDSQYNALAKDIPADRHQGA"
     gene            2098..2274
                     /locus_tag="VBC94_001894"
     CDS             2098..2274
                     /locus_tag="VBC94_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980131.1"
                     /translation="MSKKTDSNYLHSNCSDKKIWSDEAPLQQETIPKDRDCVIKPVIT
                     MTVEHVEIPLDDQS"
     gene            complement(2325..2519)
                     /locus_tag="VBC94_001895"
     CDS             complement(2325..2519)
                     /locus_tag="VBC94_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980132.1"
                     /translation="MLFLMIKPDYIRYFKVIDDMHDTMYQQTVFHVQLQAWKYTMKSI
                     DQLVVDDLNHISNEKTTLVK"
     gene            2518..2712
                     /locus_tag="VBC94_001896"
     CDS             2518..2712
                     /locus_tag="VBC94_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980133.1"
                     /translation="MIMSGQRATSTIQSDINIFHHIEAICISKSNSIGLPAIQNHLGQ
                     LIQRERLGLFFSITYSKAPT"
     gene            complement(3049..3303)
                     /locus_tag="VBC94_001897"
     CDS             complement(3049..3303)
                     /locus_tag="VBC94_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6980134.1"
                     /translation="MFVMRTFGNSLSGPLVVILSSILFSWSHLHGLSVVDFVVYFGMG
                     LIFASLHHYTKSIHYSIGEHIVWNSLSYIFYFLAFLLDLL"
     gene            complement(3322..3573)
                     /locus_tag="VBC94_001898"
     CDS             complement(3322..3573)
                     /locus_tag="VBC94_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980135.1"
                     /translation="MMILLSYVLTKTEPISNYQESSSNLKKWLFAIGAFSVLFCWALL
                     VKTFFPIRQNQEGLIADMNQVNHYFRVVLLLTICLIAPI"
BASE COUNT         1533 a          739 c          644 g         1232 t
ORIGIN      
        1 cagcacaata ttttcttact cgatttaaaa ggataaaaaa atgagtgtgc gccttagaat
       61 gaatcgacac aaattcatca atagtgatgt tgagtttgtc taaaatatta agcaaacgac
      121 tacaagtaat ttctgactct cctctctcaa accttgagat ttgtgacttg gataggtgct
      181 catctgctaa agaagaaata ctaacttttt ttcctttacg taatcttctt agtgtttttc
      241 ctaattcttt ttccattttt cccactttca caacaattta aaagaccctt taaatccatt
      301 atataataca agtacaaaat agaaaatcag aaaggagata gtcatgaaaa aaattaacaa
      361 agctttgttg ttcacattaa tcatggatat cctaattatc gttggtggat aaaactcccc
      421 acaaatggtt gttatggaat cccattttat ttagtttata acaggagaac taatgaagat
      481 taaaaaaatt attagtggct ttgccgcagc tttaattatc agttcactat caactattaa
      541 ctatgaggtt aaagctgatg acaccaccag tgagtatcac tatatcagta agcaaaataa
      601 tgaaaagcag cttattagtt acatcaagga acaacatcgt ttgctcaatc aatttgttgt
      661 tgataatgtc aactcattca ctcaactaaa tgctaatcca actattgaac agttaaatag
      721 agctataaca ttatttaaac aaaaagatga gcaattattt aaccaggtga aagctggtca
      781 tctctctccc agtaactata atgctatcgt taatcaacgt aatgtcatta accaaactgt
      841 tcaaaatctg attgaccaaa atcataataa gattcagaca agtcaaaata aagcagctca
      901 gctcgtgggg caacgtaatc aggttgttaa caaaattcaa gctattttag caactgtaaa
      961 ctacaactct gtgaattcta tacaagaagc tgaaaattta tttcattcac tcagaaatca
     1021 aattgaacct cttgtagctg aagttaataa ttacaaagct gctatggcaa tccttcaaca
     1081 agaagtagat gccctatcaa cagcggctat tgaaactgag acttctaaac ttgctactct
     1141 caaagttagc gaaaatactt ctgttcctgc aaacaaagta gaagaaaaaa ctactcaatc
     1201 agaagcgtca ggcaataaac aagaagtaac taagagtgag gaaaaacagg ctacctctga
     1261 tgcaaaggca tcacagcctg agtcagctaa tattgccgat tacgatagtt taaaagaagt
     1321 tttacgaaat aatattagca accaagtacc acacatcagt gttcaaatgg agtttaaaac
     1381 tcaagaacaa gttgacgaat accaaaaaaa tctcggaagc atcatccggg aaattggaga
     1441 tacacttgga acagcaactg aattcaatgc caaaagtaac attagcactt atactcttgg
     1501 tggacaaatc caacgcatta ttgtaaaaag cgacatcaca atcacctata ctcttaaagg
     1561 tgacatggta ggattacata aagaatataa acagtttgta gattcttttg tcaaagaaaa
     1621 tattactaac aaaaatatca caagtgatta tgaaaaagct aaagtaattc atgaccactt
     1681 ggttaataat tacacttacg cgactgaaga actggcaacc actcgtgaaa ctgctagtgg
     1741 tatcagtatc catgctcctg aagcactcta caaagataaa cgtggtgttt gtcaagcctt
     1801 tgcagtaatg tttaaagata tggctgctgc tggcttatca gtatggtatg taactggtca
     1861 agctggaggt ggaaatcacg cttggaacat tgttactatt aatggcgtta aatattatgt
     1921 tgatacaaca tgggataata atataaaaag caataaatat ttccttgttg gtaaaacaat
     1981 aatggatgct gatcatcttt tggatagtca atacaatgca ttagctaaag atattccagc
     2041 tgatcgccat caaggtgcat aactcaaaaa ttgtattgag cctggaaagg atatgaaatg
     2101 tctaaaaaga ctgattcaaa ttatctacat tccaattgtt ctgacaagaa aatttggagc
     2161 gatgaggcgc ctctgcaaca ggagactatc cctaaagaca gagattgtgt tatcaaacct
     2221 gtcattacta tgacagttga acatgtggaa atcccattag atgaccaatc ttagctctct
     2281 cctaaaataa aaaggaacac tgccattttt gacagtgttc ctttttattt aaccaaagtg
     2341 gttttctcat tactgatatg attcaaatca tctacaacta attgatcaat cgatttcatg
     2401 gtgtatttcc aagcttgcag ttgaacatga aaaacagtct gttggtacat ggtatcatgc
     2461 atatcgtcaa tgactttgaa ataccttata taatctggtt taatcattag gaacagcatg
     2521 atcatgagtg gtcaaagagc taccagcacc atccagagcg atataaatat ctttcatcat
     2581 atagaagcta tttgtataag taaatctaac agtattgggc tccccgcaat acaaaatcac
     2641 ttgggacagc tgatccaaag agaacgtctg ggtcttttct tctcgataac ctattccaaa
     2701 gctcccactt gaagtactta ctgaatctgt tgcaacagaa aaacaatccc tactactaga
     2761 ataatagcac ctctaactaa taaatgactc attccaacat tcacatcttt taacatcatg
     2821 ttcctttcat tattttttat ttaggaattc gtcatactat attttctcat atttatctaa
     2881 caaaaaactc gtaattctat ataaatcata taaagttacg aactttattt agttaacaat
     2941 aaaataataa cattttattc attaaaaaaa cgaataacaa aagagccaaa aagctctttt
     3001 aaactgacaa aaatgtctta gtaatgatta gtctctcaat cttattactt agagtaaatc
     3061 aagcaaaaat gctagaaaat aaaaaatata tgacaaacta ttccaaacaa tatgctcgcc
     3121 aatcgaataa tgaatggatt ttgtatagtg gtgcaaacta gcgaaaatca aacccatacc
     3181 aaaatagaca acaaagtcga cgacagatag accatgtaag tgagaccagc taaatagtat
     3241 agaagataag atcacaacca agggaccact cagggagttc ccaaatgttc tcatgacaaa
     3301 caacctgaac gttatctctt cttaaattgg ggcaataaga cagatagtca ataataagac
     3361 aactcgaaag tagtgattga cctgattcat atcagctatt aatccctcct ggttttgcct
     3421 tattgggaaa aatgttttta ctagtagtgc ccagcaaaat aaaacgctaa aagcacctat
     3481 tgcaaatagc cattttttga ggttagatga tgactcttgg tagttactga ttggctcagt
     3541 ttttgttagc acataactca acagaatcat cattataaga ttaatgccaa tccatatact
     3601 ccaactccac tctaacccta ttgatagtaa actaatcacc caatgcccaa gcaattgtta
     3661 ttattgtgtt gttgcaactt tggcaaagat gaatatagca ggatggaaaa cagattgttt
     3721 ctgaaacttc aaaggtatct tcttatcaca agctattatt tggttatctc cataattaat
     3781 ctaaatggag caatatatcg tttttctact aactgttgac aaataaaaaa ctccccttta
     3841 aagtctggta acttttcgat ttttaccaga aacttaagga gagtgaggca agaactaata
     3901 gaatcttatt taaagtttta tgattgggag aacagaacaa ctatcataat tactgaaaaa
     3961 ttatgaaagg catggcaaac aacagatggt caaatccgct ctgttgaaaa caaattttaa
     4021 atataattca tttcactttc taagtttatt aaacatagag tgttcaggag aaatgatacg
     4081 acctaatgat aaaatcattc ctatcgtata ataaacaacg acaaggtgta aataaaagta
     4141 aattaata
//
LOCUS       DATROQ010000126         4119 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.127, whole genome shotgun sequence.
ACCESSION   DATROQ010000126 DATROQ010000000
VERSION     DATROQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.127"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..561
                     /locus_tag="VBC94_001899"
     CDS             <1..561
                     /locus_tag="VBC94_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEO6980136.1"
                     /translation="EDHNHKDYEKIMIKALIFSIDQSIEPNQEELARLTDYLFTVEQW
                     GYYEIILLGNCSRLINYNTLFLLTKEMVNSFAYSEQNKTNKILVTHLAINCLIISIDH
                     SYFEHSHYLIDKVRSLLQDEVNFYEKTVFLYVTGYYHLKLGDTSSGKEDMRKALQIFK
                     YLGEDSFYNTYNEHYCQKVLGNNKEC"
     gene            710..1531
                     /gene="mutM"
                     /locus_tag="VBC94_001900"
     CDS             710..1531
                     /gene="mutM"
                     /locus_tag="VBC94_001900"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEO6980137.1"
                     /translation="MPELPEVETVRKGLERLVVNQEIASITIKVPKMVKTDLNDFMIS
                     LPGKTIQQVLRRGKYLLFDFGEMVMVSHLRMEGKYLLFPNKVPDNKHFHLYFKLTNGS
                     TLVYQDVRKFGTFELVRKSSLKDYFTQKKLGPEPTADTFQFEPFSKGLANSKKPIKPL
                     LLDQRLVAGLGNIYVDEVLWAAKIHPQRLANQLTESETSLLHKEIIRILTLGIEKGGS
                     TIRTYKNALGEDGTMQKYLQVYGKTGQPCPRCGCLIKKIKVGGRGTHYCPRCQCL"
     gene            1522..2115
                     /locus_tag="VBC94_001901"
     CDS             1522..2115
                     /locus_tag="VBC94_001901"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEO6980138.1"
                     /translation="MLMTKIIGLTGGIASGKSTVTKIIRESGFKVIDADQVVHKLQAK
                     GGKLYQALLEWLGPEILDADGELDRPKLSQMIFANPDNMKTSARLQNSIIRQELACQR
                     DQLKQTEEIFFMDIPLLIEEKYIKWFDEIWLVFVDKEKQLQRLMARNNYSREEAELRL
                     SHQMPLTDKKSFASLIIDNNGDLITLKEQILDALQRL"
     gene            2239..2940
                     /locus_tag="VBC94_001902"
     CDS             2239..2940
                     /locus_tag="VBC94_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6980139.1"
                     /translation="MELVIRDIRKRFQETEVLRGASYRFYSGKITGVLGRNGAGKTTL
                     FNILYGDLAADNGTICLLKDNHEYPLTDKDIGIVYSENYLPEFLTGYEFVKFYMDLHP
                     SDDLMTIDDYLDFMEIGQTERHRIIKGYSDGMKSKLSLICLMISKPKVILLDEPLTAV
                     DVVSSIAIKRLLLELSEDHIIILSTHIMALAEDLCDIVAVLDKGKLQTLDIDRKHEQF
                     EERLLQVLKGDEYDK"
     gene            3165..>4119
                     /locus_tag="VBC94_001903"
     CDS             3165..>4119
                     /locus_tag="VBC94_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001882372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980140.1"
                     /translation="MKQLNILPQEGFTLTLTLMVITSLLIVINFYIKLIEPNDMQQTH
                     MIRLFRIEPSRYFQSAELLEASSELFFRSLSFGIFFHLNHFAFWHGLTFALFLAGSRL
                     GIKVLCLPLYGRGKDQENPESLLNILFYLGIGIGVFLSLFFLLLGKQFSPYPFFSWYT
                     GIAGLIIWLVSYYRLKTYSKLNHLTYLTLTHETMKEKIAKIDNIELLGIEMKDRDINV
                     SELSPLLFENLSGISYLNAIFLKRMSRYLQRKILVRLAVLSVLFGIELLFLLFFKEKI
                     NIPMNEHSFSKFLFLSAVPGYLIYIGESYSKFCFYHLDRPLL"
BASE COUNT         1394 a          599 c          759 g         1367 t
ORIGIN      
        1 gaggatcata accataaaga ctatgaaaag attatgatta aggctcttat tttttcaatt
       61 gatcaatcaa tagagcctaa tcaagaagaa ttagcacgct taacagacta tctttttacg
      121 gtggagcagt ggggatatta tgaaattatt ctcttaggaa attgctcacg tctcattaat
      181 tacaatactt tatttttatt gaccaaagaa atggttaatt cgtttgctta ttcagaacaa
      241 aataaaacca ataaaatcct agttacacac ttagcaatta attgtctaat catcagcatt
      301 gatcactctt actttgagca tagccactac ttaattgata aggttagatc attgttacag
      361 gatgaagtta atttctatga aaaaactgtt ttcttatatg taactggata ctatcattta
      421 aaacttggtg atacttcttc aggaaaagaa gatatgagga aagctttaca gatttttaaa
      481 tatttaggtg aagattcatt ttacaatact tataatgaac attattgtca aaaagtactt
      541 ggcaataata aagaatgcta atgagtgcaa gtttatgagt aatttttaaa gcttttgtct
      601 caaaatgata ataagaaagg aaaatagaaa tcgaatggtc aggtgacatc aagaacgttt
      661 ggaaattatt gctctaagca atgactattg atttttagac tatatattta tgccagaatt
      721 accagaagta gaaaccgtta gaaaaggact agaaagactt gtagttaatc aagaaatagc
      781 ttctataacg attaaagtcc ctaaaatggt gaaaactgat ttgaatgact ttatgataag
      841 tctaccagga aaaacaattc aacaggtatt gagacgaggg aagtatcttc tttttgattt
      901 tggtgaaatg gttatggtat cacatttacg catggaagga aaatatctac tcttcccaaa
      961 taaagtacca gataacaaac attttcacct ttatttcaaa ttgactaatg gcagcacatt
     1021 ggtttaccaa gatgtccgta aatttgggac atttgagttg gttcgtaaat catctctcaa
     1081 agattatttt actcaaaaaa aattaggtcc tgagccaact gctgacacat ttcaatttga
     1141 gcctttttca aaaggcttag caaactccaa aaagccaata aaacctttac tattagatca
     1201 gagattagta gcaggacttg gaaatattta cgtagatgaa gtcttgtggg cagcaaaaat
     1261 tcacccacag cgtttggcta atcaattgac cgaatctgaa acaagcttat tacacaagga
     1321 aattattcgc attttaactt taggcatcga aaaaggtggc tccacgatta gaacatataa
     1381 gaatgctctt ggagaggacg gtacgatgca aaagtattta caggtctatg gtaaaactgg
     1441 gcaaccgtgt ccaaggtgtg gttgtttaat taagaagata aaagtaggag ggagagggac
     1501 gcattattgc cctcggtgtc aatgcttatg acaaaaataa taggactgac aggagggata
     1561 gcttctggaa agtcaacggt aacaaaaata atacgagaat caggttttaa agtcatagat
     1621 gcggatcaag tggttcataa attgcaagct aagggtggga aactttacca agctttatta
     1681 gaatggttgg gtcccgagat acttgatgct gatggtgagt tggatagacc aaagctttct
     1741 caaatgattt ttgctaatcc agacaatatg aagacatcag ctaggctaca aaatagtatc
     1801 attcgtcaag agttagcatg tcagcgcgac caattaaaac aaacagaaga gatatttttc
     1861 atggatattc ctttattgat tgaagaaaag tatataaaat ggtttgatga gatttggttg
     1921 gtatttgttg ataaagaaaa acaattacaa cgattaatgg cccgtaacaa ctacagtcga
     1981 gaagaagcag aattacgact ttcacaccaa atgcctttaa cagataaaaa aagtttcgct
     2041 agtcttatta ttgacaataa tggtgattta ataactttaa aagagcaaat attggatgct
     2101 cttcaacgtt tatagaacta ctaaatggat aatgtattta tccatttttt gtctttatta
     2161 cactagtttt aaaaaacttg cgattaaacc acagtaatag tacaatgata taaaaggttt
     2221 actttcaaag gagtttttat ggagttagta attagagata ttcgtaagcg gtttcaggaa
     2281 acagaggtct tgagaggagc aagttaccga ttttattcag gtaaaataac aggggtctta
     2341 ggtaggaatg gtgctgggaa aacaacttta tttaatatac tttatgggga tcttgcagct
     2401 gacaacggga ccatttgttt attgaaggat aatcacgagt atcctcttac cgataaggat
     2461 attggtattg tttattccga aaactacctt ccagaatttt taacagggta tgaatttgta
     2521 aaattttaca tggatttaca tccttcagat gatttaatga caatagatga ttatttagat
     2581 tttatggaaa taggacaaac agagcgtcat agaattatca aaggatattc tgatggaatg
     2641 aagagtaagc tctcattaat ttgcctgatg atttctaagc caaaagtaat tttactagat
     2701 gagccactga ctgcagttga tgttgtatca agtattgcaa taaaacgcct tttgttggaa
     2761 ttaagtgagg atcatattat tatattatca actcatataa tggccttagc agaagatcta
     2821 tgtgatattg tggctgtatt agacaaagga aaactccaaa cattagatat tgatcgtaaa
     2881 catgaacaat tcgaagagcg tcttcttcaa gtgttgaagg gagatgaata tgacaagtaa
     2941 agcattaata tggtctatat tacagattga tagtgctaga gcttataata gttttttata
     3001 ttaccttcag aaaatcccct ggttgggaag aaaaatccct aaccgatggt ataaaaggtg
     3061 tagtactatt tattcgtatc attttctttt tcttaagagg ttttggtcgt tccataggtt
     3121 acgtactata ttgttatgtt ttatcatttt ttgttagaag tggcatgaag cagttaaata
     3181 ttttaccgca agaaggtttt accctaactt taacattgat ggtgattacc tctcttttaa
     3241 ttgtcataaa tttctatata aaacttatag aacctaatga tatgcaacaa actcatatga
     3301 ttcgcctttt cagaattgag ccatcgaggt atttccaatc cgctgaactt ttagaagcca
     3361 gtagtgaact attttttaga agtttgagct ttggcatatt tttccattta aatcactttg
     3421 ctttttggca tggtttgact tttgctttat ttttagcagg tagtcgcctt ggaatcaagg
     3481 tgctctgttt acctttatat ggtagaggta aagaccaaga gaatccagag tctttactaa
     3541 atattttatt ttacttaggg attggtatag gagtcttcct ttctctcttt ttccttcttc
     3601 ttggcaaaca gttctctcca tatccatttt tttcatggta caccggtatt gcaggactta
     3661 tcatatggtt agttagttac tatcgattaa aaacttattc taaacttaac cacttgacct
     3721 accttaccct aacgcatgag acgatgaagg agaaaattgc aaagattgat aatatagagc
     3781 tgcttgggat tgaaatgaaa gatagggata taaatgtcag tgaactttct cccctgctat
     3841 ttgaaaattt atcaggtatt tcatatttaa acgctatctt tttaaaacgg atgagtagat
     3901 acttgcaaag gaaaattttg gtacgtttag ctgttttaag tgttctgttt ggaatagaac
     3961 tccttttcct gctatttttt aaagaaaaga taaatattcc gatgaatgag catagttttt
     4021 caaaattcct atttttgagt gcagttccag gatatctaat atatatcggt gaatcctatt
     4081 caaaattttg tttctatcat ttggatcgtc cattattaa
//
LOCUS       DATROQ010000127         4079 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.128, whole genome shotgun sequence.
ACCESSION   DATROQ010000127 DATROQ010000000
VERSION     DATROQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.128"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(71..427)
                     /locus_tag="VBC94_001904"
     CDS             complement(71..427)
                     /locus_tag="VBC94_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEO6980141.1"
                     /translation="MSTLTIIIATLTALEHFYIMYLETLATQSNMTGKIFSMSKEELS
                     YLPVIKLFKNQGVYNGLIGLFLLYGLYISQNQEIVAIFLINVLLVAVYGALTVDKKIL
                     LKQGGLPILALLTFLF"
     gene            complement(494..2812)
                     /locus_tag="VBC94_001905"
     CDS             complement(494..2812)
                     /locus_tag="VBC94_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEO6980142.1"
                     /translation="MLDELKAIIKSPKLWITMAGVALIPTLYNVIFLSSMWDPYGNTK
                     NLPVAVVNQDKSAKLNGKTISIGKDMEDNLSKNDSLDFHFTTAKRAEKELEKGHYYMV
                     ITFPKDLSRKATTLMTEKPERLNITYKTTKGRSFVASKMSETAANKLKDEVAESITGT
                     YTESVFKNMGSMKTGINKAADGSQELLNGSNKLQNGSQTLTSNLDVLASSSQTFSGGA
                     NKLNSGINLYTDGVGTLSNGLETLSDGVTAYTTGVHKLSEGSQKLDDKSQALVEGSEK
                     LTVGLQQLSQATQLKPEQERTLQNLSDGLKNLNQIITNLQSTATTDSDTNSKLFNFLS
                     TIESSTTALMNTAAADKQKQMTAVQSTSAFKSLTPEQQSQITSAVTGTPTSAETIAAN
                     ISSNIENMKTVLSEASSSAPSNNNSQNFQTLSGTANNLVLKAISDLDKIQKLPTATKQ
                     LYQGSQTLTKGITDYTNAVGQLRKGAETLDSKSNQLISGTQKASQGAQTLDSKSDQLR
                     DGAGQLASGSDRIADGSNKLAGGGHQLTDGLTELSGGVSQLSSSLGKAGDQLSMVSVN
                     KDNANAVSSPVTIKHEDYDSVDTNGVGMAPYMISVALMVVALSANVIFAKALSGKEPA
                     NRFSWAKNKLLINGFIATLAATILFFAVQFIGLKPDYPGKTYFIILLTAWTLMALVTA
                     LVGWDNRYGSFLSLLILLFQLGSSAGTYPIELSPKFFQTIQPFLPMTYSVSGLRETIS
                     LTGDVNHQWRMLVIFLVSSMILALLIYRKQED"
     gene            2951..3490
                     /locus_tag="VBC94_001906"
     CDS             2951..3490
                     /locus_tag="VBC94_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEO6980143.1"
                     /translation="MVNDTRREKTKRAIEAAMITLLKDQSFDEISTINLTKTAGISRS
                     SFYTHYKDKYEMIDQYQQSLFNKVEYIFDRNQFKKEDALLEIFQFLDRESLFAALLTQ
                     NGTKEIQTYILNKLQLMLSKELPVVNPDATKSDINRLYYSVYLSHAIFGVYQMWITRG
                     KKESPQQITQVLLSLLPQT"
     gene            complement(3576..3872)
                     /locus_tag="VBC94_001907"
     CDS             complement(3576..3872)
                     /locus_tag="VBC94_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEO6980144.1"
                     /translation="MNLNDRLKIEEMEEKYDSFKPRINALVEAIDDFQKHYEDYVKLR
                     EFYGSEDWFRLSEQTENNLKCGVLSEDQLFDFIGEHNELVGQFLDMSSQMYRHL"
BASE COUNT         1325 a          748 c          608 g         1398 t
ORIGIN      
        1 aaactacctg tattatgaga aaaaaatgat tactaaaagc cgttcaacta aatcgaacgg
       61 ctaagtagta ttaaaataag aatgttaaaa gagctaatat aggtaaacca ccctgtttta
      121 ataagatttt tttatcaact gtcaaagcac cataaacagc aactagcaac acattgatta
      181 aaaaaatagc tacaatttct tgattctgtg aaatatataa cccataaagg aggaatagac
      241 caatcaagcc gttgtataca ccttgattct taaaaagttt aataacgggt aaatatgaca
      301 actcttcttt agacatacta aaaatcttcc cagtcatatt tgactgggtg gctaacgtct
      361 ccaaatacat aatataaaaa tgttccaaag cagttaatgt tgcaataatt attgttaaag
      421 tagacatatc ttctcctcct aaatactttg acatagcata tcatactgtt agtctatcac
      481 tagatacttt ctattaatct tcttgtttac gataaataag aagagcaagt atcatcgatg
      541 atactaaaaa gattactagc attctccatt gatggttaac gtctcctgtc aacgagatgg
      601 tctcccttaa tcctgaaaca gagtaagtca tcggtaaaaa tggttgaatt gtttgaaaga
      661 acttaggact caattctatt gggtaagttc ctgcgcttga accaagctgg aataataata
      721 ttaataacga caagaaggaa ccatacctat tatcccatcc cactaaagca gttactaatg
      781 ccatcaaagt ccatgctgtc aataggataa taaagtaggt ttttccaggg taatctggtt
      841 taagacctat gaattgaact gcaaaaaata agatagttgc tgctagagtt gcaatgaatc
      901 cattgattaa taatttattt ttagcccaac taaaacgatt agctggctct ttgccagata
      961 aagctttcgc aaaaataaca ttggctgata aagctaccac catcaacgca actgaaatca
     1021 tgtatggtgc cattccaaca ccattcgtat caacgctatc ataatcttca tgcttgatag
     1081 tgactgggct agatacagca ttggcattat ccttgtttac agaaaccatg gacaactggt
     1141 ccccagcttt acccaaagaa cttgataatt gagaaacacc acctgacaac tctgtcaacc
     1201 catctgtcaa ctgatgtcct cccccagcaa gcttattaga accatctgca attctatcag
     1261 aaccactagc taattgcccc gctccatctc tcaattgatc cgatttacta tctaaagttt
     1321 gagctccctg acttgctttt tgtgttcctg aaatcaattg attggattta ctatctaaag
     1381 tttctgcacc tttgcgtaat tgccctacag cattcgtata atccgtgatt ccttttgtca
     1441 aggtttgact accttgatat aactgcttag tagccgttgg caatttttga attttatcta
     1501 aatctgagat agcttttaat actaaattgt tagctgttcc tgacaaagtt tgaaaatttt
     1561 gtgagttatt attagaaggg gctgaactag atgcttcact aagcacagtt ttcatatttt
     1621 ctatatttga agaaatattt gctgcaattg tctcagctga agtaggtgtt ccagtaaccg
     1681 ctgaggtaat ttgagattgt tgttcagggg ttaaactttt aaaagctgac gttgattgta
     1741 ccgcagtcat ttgcttttgt ttatcagcag cagccgtatt catcaaagcc gttgtacttg
     1801 attcaatagt tgataaaaaa ttaaataact tactattagt atcgctatca gtagttgctg
     1861 tcgattgtaa atttgtaata atctgattta gattctttaa tccatctgaa aggttctgca
     1921 aagtacgttc ttgctctggt tttagttgag ttgcttgtga caattgttgt aaaccaaccg
     1981 tcaacttttc tgatccttcc accaatgctt gagacttatc atctaatttt tgactacctt
     2041 cactcaactt atgcacacct gttgtgtaag cagtaacacc atctgataat gtttccaacc
     2101 cgttagatag ggttcccaca ccatctgtat aaaggtttat tccactattt agtttattag
     2161 ctcctccaga aaaagtttga ctagagcttg ctaaaacatc taaattactt gtcaaggttt
     2221 ggctcccatt ctgtagctta ttagaaccat ttaataattc ttgactccca tcagcagctt
     2281 tattgatacc tgttttcata gaacccatat ttttaaaaac tgattcagta taagtccctg
     2341 taatagattc tgctacctca tcttttagtt tattagctgc tgtctcactc atttttgaag
     2401 ctacaaaact acgcccctta gtagttttat aagtaatatt taacctttct ggtttttctg
     2461 tcatgagagt tgtcgctttt ctagaaagat ctttaggaaa ggtaataacc atataatagt
     2521 gacccttttc taattctttt tctgctcttt tagctgttgt aaaatggaaa tctaagctat
     2581 catttttaga caaattgtcc tccatatctt tgccaattga aatagttttt ccatttaatt
     2641 tagccgattt atcttggtta acgactgcca caggtaaatt tttagtattc ccataaggat
     2701 cccacattga gcttagaaag ataacattat atagcgttgg aatcagtgca accccagcca
     2761 tcgtaatcca taattttggg ctttttatga ttgcttttaa ttcgtctaac attttttctc
     2821 ctattttgga catattgttc atttttttga taaaatatat tatacagtga tgatagataa
     2881 tttcaagata aaaatctcgt ttttggacac tgtgtttaaa aatgtacaaa aatattaagg
     2941 ggtaattaat atggtaaacg atacgaggag agaaaaaaca aaaagagcta ttgaagcagc
     3001 tatgattacg ttattaaaag atcagagctt cgatgaaatt tcaacgatta atctaactaa
     3061 aacagctgga atcagtcgtt ctagttttta tactcattat aaagataaat atgaaatgat
     3121 tgatcaatac caacagtcac tcttcaataa agtcgaatat atttttgatc gcaatcaatt
     3181 taaaaaagaa gatgccttgc tcgaaatttt tcaattctta gatagagaat cattatttgc
     3241 tgctctctta acccaaaatg gaacaaaaga aattcagacc tacatcctta ataaattaca
     3301 attaatgctt tctaaagaat tacctgtggt caatccagat gcaacaaagt ctgatatcaa
     3361 ccgtttgtat tatagtgtct atctcagtca tgctattttt ggagtctatc aaatgtggat
     3421 aacacgagga aaaaaagaaa gccctcaaca aattacacaa gtgctactca gtttattacc
     3481 tcaaacttaa aaccagcctt ccttataggc tggtcatttt tagttaggtt tattttatcc
     3541 tcattatagg ataacaacac taataaggac tgacattata aatgacgata catttgagaa
     3601 gacatatcta aaaattggcc tacaagctcg ttatgttctc ctataaaatc aaagagctga
     3661 tcctcactca gtacaccgca tttgagatta ttttctgttt gttctgatag tctaaaccag
     3721 tcttcgctac cataaaattc tcttaatttc acgtaatctt catagtgctt ttgaaaatca
     3781 tctatagctt ctactaatgc attgattcta ggtttgaagc tatcatactt ctcttccatt
     3841 tcttctattt ttaatctatc atttagattc ataataaaat ctccttagtt atataagatt
     3901 cgacgagata agttatcatt ctcaagtatt tgtaacgtta actctactat atccttttta
     3961 caaagtaaaa aattactcat tacaaactga cagtgttgat ttttattcta atgttagttt
     4021 aaaatctttt tggttcatta aatataatga ctcactaaaa agtatatagc aaaaaaagc
//
LOCUS       DATROQ010000128         3857 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.129, whole genome shotgun sequence.
ACCESSION   DATROQ010000128 DATROQ010000000
VERSION     DATROQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.129"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(290..2758)
                     /locus_tag="VBC94_001908"
     CDS             complement(290..2758)
                     /locus_tag="VBC94_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6980145.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGLATKDNQIAYIDD
                     SKGKVKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYHFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTDDNDSGSDHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELVAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YIMPKDGKDYFYARYQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRNQIATIKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFAAPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPEEKQQAD
                     KSNENQQPSEASKEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQPE
                     GVQFYNKNGELVTYDIKTLQQINP"
     gene            complement(2771..3691)
                     /gene="lmb"
                     /locus_tag="VBC94_001909"
     CDS             complement(2771..3691)
                     /gene="lmb"
                     /locus_tag="VBC94_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEO6980146.1"
                     /translation="MKKVFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVNVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGHLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
BASE COUNT         1025 a          801 c          710 g         1321 t
ORIGIN      
        1 gaatttttat aacaattaca acccacatac ggctaatcta ggactaaccc ctaatcagaa
       61 agaagaaaat tattttaact caataaaata acacagtttt ctgtctactt atttgacatt
      121 agtccaagaa tcctcctctt tttttactat tctaacatgg ataccatttc tttgcaaaag
      181 aaaaccgcta ttatccaact tttatcacta ctcgttacat agaatgactt caaaatcacc
      241 gtaacttgct ttgacaaagc agtgctttaa caatgagatc ttcttttggt taagggttta
      301 tttgttgaag tgtcttgata tcataagtta ccaattcacc atttttatta taaaattgga
      361 caccttctgg ttggaaaatg agatacttag gatcgatatt agctttttgt gctaattgat
      421 tgatatgatc ttctagggtt gctctatcta gaccatagtc tggtaaactg tctataaagt
      481 catctgattc tttttcttct ttactggctt cacttggctg ttggttttca ttgctcttat
      541 ctgcctgttg cttttcttca ggtttatccg tatcagtagc atcaccagca tttttctttg
      601 ccaataactc ttgagcttgt tgccactcag cttgggatag atcggattta ttaatggttc
      661 tcaatgaact gccatctgct cttgggatgc taaaggagcc atctttccat acaaaagttt
      721 cttttgccaa aacatctcgt ggatcgaaaa tatagccgtc tggtgctgca aaacgacctt
      781 ctgctagggc tttttgaact tcttcagcag aatggataat ttgccagttt ggcataccag
      841 cacgtttatc aagaggcgta acatttggaa tgaccgaacc tgactcttca tgccctggct
      901 tagaccatac atccggacga acttcggggt gttgcatcac atacttgatt gttgcaatct
      961 gattgcgcgt caaccatgaa tacggaacga catggatatg atcaatatgt gggataacaa
     1021 acgaacttcc agtatcgtaa gtagcattac cagcatgcat cggcagactt gacacatcta
     1081 cagcaagtcg tggctcaatg cctgtatcaa caatgtcata acggtaatgc ttcttatctt
     1141 taagcattag ttcttgttcg gcaaaggcaa tctgagtcaa atcaagttga taacgagcat
     1201 agaaatagtc cttgccatct tttggcataa tatagcccac tttaccatct tttgttactt
     1261 tgcgactcac ttttttagtg tcaaagagtg gtttttcttt gccttgttcc tgatccaaag
     1321 cagcaacaag ctcatcagct tgaccttttg ctttcaccca gttagcgacc tcatccaact
     1381 catattgttc aagttctcca aatcctatat agtggaaatg atctccgtgt ttagctgtaa
     1441 ctcctgattt atccactgaa tgaatggatt ctttactaaa aacataagca tcactcgtat
     1501 catatggttt accatctaag ccttttccgt aagctttgat gcgatgacca agaaaggtat
     1561 gtgtcacttc tttatctgat ggttttgagt gatctgaacc tgagtcgttg tcatcagttt
     1621 ggccggctaa gtatcgatct gctaattcca tttcaagagg tgataactga cttcttggga
     1681 taatatgata atgatctcca tgaggcacca catacccaaa agcgtttgat ttgatcactt
     1741 gagtcggttc aaaaatcaac ccatcttctt ccacatgacg gtatttcaaa tcaagacggt
     1801 gtagttgatc taaaagttcc ttaaaggttt ttcctttaaa ctcatctctt tggtgtttgt
     1861 tttgtgacgc atcatttggc ctaggaggcg ctggatgata accaccgtta tctggctgat
     1921 gaccttgtcc agggttaggc gtcacatcag gaattggggc tttcctacga cctggggctg
     1981 gtgtcgggcg gtaatcagac ggtctagcac ctcgaccttg tttttgactc cagtaggctt
     2041 gtgcagcagc tagctcactt ggagacaaat cttttttagg aatataatga tagtgattac
     2101 catgaggtac taaataagca tctcctaaat catcaatgat atctgtcgga ctaaaaatat
     2161 agccatcgtc tgtagtatag cgtccttgtc tttttgcttc attgactgcc gcaacttctt
     2221 ctttactgag atgggccact tgagctaaac ctttttcttt agcttcttta gttcctttgg
     2281 ctacttgctc agcaatttgt tgtttggttc gaatgttttt gcgcttacta cctggcttga
     2341 ggtaaacata atagttgcca ttgactttaa taacgtaacc gtctaagatt tcattgataa
     2401 cgtctgattg tttaaaatgg taattaggat ccgtcatcaa caactcttca ctaataatcg
     2461 catcataagg aactttccca ttgtaaaaat gataatggtc accgtgtgag gtaacataac
     2521 cttggtcagt aattttgact acgatctgtt cagcagagat gccttcttca gcactgattt
     2581 gatccatcgt tttgtttgtt ttaggggctt ttaccttacc tttgctatca tcaatatagg
     2641 caatctgatt gtcctttgtt gctagaccca tatgatgctt accaagctgg taacttccaa
     2701 tatgagtagc tagtaaaata gcagcaactg agccgatata accatatgtt ttcttcacta
     2761 ataatctcct ttacttcaac tgttgataga gcacttccaa atttgctcta agattttcta
     2821 gatatgtctt gtttccgctt ggagcagctt caagtggact taatgtcttt actttagctc
     2881 ctgttgattt cgcaatagca tgagcaattt tggggttgac gttgtcttct gcaaaaatag
     2941 tcttgacgtt gtattcttta acaaagtctt gaatttcttt caattggcga ggagagggct
     3001 cttgctctgg agaaataccc gagataccaa gttgtttcaa gccgaatcgt ttagccagat
     3061 aagaaaatgc cgtgtgttgc gtcacaaatg tttttgagcg caccttttta aatttttgag
     3121 tgtattcttc agttagttgc tctgcttctt ttttgaaagc cttagccttt ttagtgtaac
     3181 tgtctttgtg tttaggatcc aaatgtccta gctctttagc gatattaaca gcttcctcac
     3241 cagctaaaac gggatccgtc caggtatgtg ggtcataaag tgtcgcaggg tcaatgcctt
     3301 gtgtgacttc catatcttct agccctttga ctctatctag tgtcagaggt tttgacgctt
     3361 caaacacatt aacctttgat ttttttaaat tagggtctag atcccttgcc caagcttcta
     3421 aggtatgtga atggtaaaca aacaaatccg cgtcataaat agctgccaca tcatttacag
     3481 acggttcaaa ggaatgaatg cctgcacctg attggatcat cctcacatca tttaggtctc
     3541 cagatacttc ttttgtcatc gcatacattg ggtaaaagct ggttacaact gacatgcctt
     3601 gcgtaggctg tttggggttt gctgacttat cacaccctgc tatcattact aaactcacaa
     3661 ccatagccat gagaaaaaaa acttttttca tagtacctcc tcaattataa tttaaccagt
     3721 tattaaccag ttaattttac tcctttatca atgctttgtc aatgacaact accatctttt
     3781 atcacatcaa ctgaagggat aagcaaaagg caattgttga cagctatttg tcctaacttc
     3841 tttttgatga ggtagca
//
LOCUS       DATROQ010000129         3845 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.130, whole genome shotgun sequence.
ACCESSION   DATROQ010000129 DATROQ010000000
VERSION     DATROQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.130"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            349..1116
                     /gene="cfb"
                     /locus_tag="VBC94_001910"
     CDS             349..1116
                     /gene="cfb"
                     /locus_tag="VBC94_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfb"
                     /protein_id="HEO6980147.1"
                     /translation="MNVKHMMYLSGTLVAGALLFSPAVLEVHADQVTTPQVVNHVNSN
                     NQAQQMAQKLDQDSIQLRNIKDNVQGTDYEKPVNEAITSVEKLKTSLRANPETVYDLN
                     SIGSRVEALTDVIEAITFSTQHLTNKVSQANIDMGFGITKLVIRILDPFASVDSIKAQ
                     VNDVKALEQKVLTYPDLKPTDRATIYTKSKLDKEIWNTRFTRDKKVLNVKEFKVYNTL
                     NKAITHAVGVQLNPNVTVQQVDQEIVTLQAALQTALK"
     gene            1288..1590
                     /locus_tag="VBC94_001911"
     CDS             1288..1590
                     /locus_tag="VBC94_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO6980148.1"
                     /translation="MTKKITVVALETLIAQHNNIHLIDVREEHEYRGGHIPGAINLPL
                     SQLSHKFEQLDKNKEYYLVCQRGGRSIRACQFLELKGYKVINVDGGTEAWRGNLEV"
     gene            complement(1747..2610)
                     /locus_tag="VBC94_001912"
     CDS             complement(1747..2610)
                     /locus_tag="VBC94_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6980149.1"
                     /translation="MNQEKKRLNLIGLILLAQAIVLSLATTLFAEILQNDVWIGIAST
                     LIALLIPCFFMGFKNLKKHLTATHHTITFTTILFLLSIIYTTNNIVSTIWDAVLNHLN
                     IMESESQNYDATFSILMISYSVVVGPFFEEVLYRGIVLNLLSKYGKWFAIITSGILFG
                     LMHQDISQLLTTSIAGIIMGFIAYHYSFKVALLLHICNNFIVEIFTQLSTVNELYGTY
                     FENILLILAILFILYYLFTHRNTARRRISLHSNVREADSINSKQHTKLLLTSWPFILL
                     VIYDIVLTTIN"
     gene            complement(2915..3475)
                     /locus_tag="VBC94_001913"
     CDS             complement(2915..3475)
                     /locus_tag="VBC94_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEO6980150.1"
                     /translation="MRITIFCGASTGENPVYSEKTVALAQWMAQNKHALVYGGGKVGL
                     MGVMADTVIANGGYTTGVIPTFLRDREIAHENLSELIIVNNMPERKAKMMLLGDAFIA
                     LPGGPGTLEEISEVISWSRIGQNDNPCILYNVNGYFNDLKNMFDHMVSEGFLSLEDRE
                     NVLFSDDIAEIEDFITNYKVPSTRQY"
     gene            complement(3472..3789)
                     /locus_tag="VBC94_001914"
     CDS             complement(3472..3789)
                     /locus_tag="VBC94_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEO6980151.1"
                     /translation="MDITDYQKWVSEFYKKRNWYQYNSFIRSNFLSEEVGELAQAIRK
                     YEIGRDRPDETEQTDLENLNDIKEELGDVLDNIFILADQYNISLEEIISAHRTKLEKR
                     FET"
BASE COUNT         1361 a          584 c          672 g         1228 t
ORIGIN      
        1 tttttttaga aaaacattag gctaacctat ttgataaaaa tagtgataag tagaaatttt
       61 ctaaaatatt tagatttata taaggtggta aattatcgtt atggtttttt acatgaaaat
      121 ctaaattaaa ttatcagaaa ttaacaaaag gataggcgca agtatattat agcaaaatga
      181 taatattagt tttatgaaaa aaatgcagtt tatttgtaat aatgtaaaat ataggggaaa
      241 agaaagcgct tgacgacctt ttggacaagt agtaagatac aaacatgggc cctgtaaatt
      301 aaaaatactg cagtaaaagt gattttagtt taaaggagga aatttattat gaacgttaaa
      361 catatgatgt atctatctgg aactctagtg gctggtgcat tgttattttc accagctgta
      421 ttagaagtac atgctgatca agtgacaact ccacaagtgg taaatcatgt aaatagtaat
      481 aatcaagccc agcaaatggc tcaaaagctt gatcaagata gcattcagtt gagaaatatc
      541 aaagataatg ttcagggaac agattatgaa aaaccggtta atgaggctat tactagcgtt
      601 gaaaaattaa agacttcatt gcgtgccaac cctgagacag tttatgattt gaattctatt
      661 ggtagtcgtg tagaagcctt aacagatgtg attgaagcaa tcactttttc aactcaacat
      721 ttaacaaata aggttagtca agcaaatatt gatatgggat ttgggataac taagctagtt
      781 attcgcattt tagatccatt tgcttcagtt gattcaatta aagctcaagt taacgatgta
      841 aaggcattag aacaaaaagt tttaacttat cctgatttaa aaccaactga tagagctacc
      901 atctatacaa aatcaaaact tgataaggaa atctggaata cacgctttac tagagataaa
      961 aaagtactta acgtcaaaga atttaaagtt tacaatactt taaataaagc aatcacacat
     1021 gctgttggag ttcagttgaa tccaaatgtt acggtacaac aagttgatca agagattgta
     1081 acattacaag cagcacttca aacagcatta aaataatatt tgtatttttc gtgtgatgct
     1141 gtcgacttcg tgattttgta ctaccatgat tgttatgatt aaaagattac gacaatagtc
     1201 ataatagtag aacgatgtca ccattttaaa taataaagtg attagtcatt tgactaaatt
     1261 tgccaagtat caaaggaaat aaagattatg actaaaaaga taactgttgt agcattagaa
     1321 acattgattg cccagcataa taatatccat ttgatagacg ttcgtgaaga gcatgagtat
     1381 cgtggagggc atattccagg tgcgataaat cttcctttga gtcaactcag tcataagttt
     1441 gaacagttag ataaaaataa ggaatattat cttgtttgtc aacgaggggg aagatctatt
     1501 agagcatgcc aatttctcga gttgaaaggc tacaaagtaa tcaatgtaga tggtggcaca
     1561 gaagcgtgga gaggaaatct agaagtgtaa atatgtcctg tgtagtctac catatttgat
     1621 aataaaaaaa ttgacattat ctcactatta atgacctttt agtaaacata aagaaaagta
     1681 tgtgttttga aaaggaggat gattatcctt ttcaaaacca tactttttgt gtcttgtggc
     1741 ttggtcttaa ttaatagttg ttaggacaat gtcgtaaatg acaaggagaa taaaaggcca
     1801 actagtaagt aataattttg tgtgttgttt agagtttatt gagtcagcct ctcgaacgtt
     1861 agagtgcaga cttatacgac ggcgagcagt attcctatgc gtaaaaaggt aatataatat
     1921 aaataagatt gctaaaatta gcaatatatt ttcaaaataa gtcccgtata actcgtttac
     1981 tgttgatagc tgggtgaaaa tttctacgat aaagttattg cagatatgaa ggagtagggc
     2041 taccttaaag gaataatggt aagcgataaa acccattatg atacccgcaa tagatgtagt
     2101 caatagctga gaaatgtctt ggtgcattaa accaaatagg atgccagaag tgataatggc
     2161 aaaccattta ccatacttgg ataataaatt caatactatt cctcggtata aaacttcttc
     2221 aaaaaaagga cctacaacaa cagaataact aatcataagt atactgaatg tggcatcata
     2281 attttgactt tccgattcca taatattgag atgatttagc acagcatccc atattgtgga
     2341 gactatatta ttagtagtat atataatgct taacaagaat aaaatagtgg tgaaagtgat
     2401 tgtatgatga gttgccgtta gatgtttttt taaattttta aaacccataa aaaaacaagg
     2461 aatgagtaag gcgattaaag tacttgctat acctatccag acatcatttt gtaagatttc
     2521 agcaaatagt gtagtcgcca aacttaaaac aatagcctga gctaaaagta tcagcccgat
     2581 taggttaagt ctcttttttt cttgattcaa ttgattacct ccttattaat aacggtaacg
     2641 acaaagaaat attataatgt ataatgtata ggtttctaag tcattagtca atagggacga
     2701 gcacttggat acttctaata agataaaata catagacaag actatggttt tggcttctct
     2761 aaagattacc taaaaattta taagttctcg agcagaaagg atgagtttgg atattgagag
     2821 gatgggcttg accagctgaa agaaaaatga accaagggta atcgcttcga aaaaagaagg
     2881 aaatttcaat tagaaacttc ctttttttat caatctagta ttggcgcgtg ctagggactt
     2941 tataattcgt tataaaatct tcgatttcag caatatcatc tgagaatagg acattttctc
     3001 tatcttccag actaagaaaa ccctcactaa ccatatgatc aaacatattt tttaaatcat
     3061 taaagtagcc attaacatta tataaaatgc aaggattatc attttgaccg atgcgagacc
     3121 aagagataac ttcggaaatt tcttcaaggg taccaggtcc accaggtaga gcgataaagg
     3181 catctccaag tagcatcatt tttgccttac gttcaggcat attatttact atgataagct
     3241 ctgataaatt ttcatgtgca atttcacgat ctcttaaaaa tgtcggaata acaccagtag
     3301 tatagccacc atttgcaatc acagtatcag ccatgactcc cattaagcca acttttcctc
     3361 caccatacac cagagcatgt ttgttttgtg ccatccattg tgccaaggca actgtttttt
     3421 cactataaac aggattttcc ccagtacttg ctccacaaaa aatagttatt ctcatgtttc
     3481 aaacctcttt tctaatttag tgcgatgtgc gctgataatc tcttctagtg agatattata
     3541 ctgatcagca agtatgaaga tattatctaa gacatctcct agttcttctt ttatatcatt
     3601 taagttttcc aaatcagttt gttcggtttc gtcaggtctg tcacgtccaa tttcatattt
     3661 gcgaatggct tgagccaatt cgccgacttc ttctgaaaga aagttacttc taataaaaga
     3721 attgtattga taccagtttc tttttttata gaattcgctc acccattttt gataatctgt
     3781 aatatccatt gttccctccg tttcaagtat attgtagcaa tattgcttta ttaatgaaaa
     3841 tttga
//
LOCUS       DATROQ010000130         3756 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.131, whole genome shotgun sequence.
ACCESSION   DATROQ010000130 DATROQ010000000
VERSION     DATROQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.131"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            212..1288
                     /locus_tag="VBC94_001915"
     CDS             212..1288
                     /locus_tag="VBC94_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6980152.1"
                     /translation="MLGENIYLQRTQIGMTQENLSDYLHLTKTTISKWENNQAKPDID
                     YLILMANLFDISLDDLVGYQKTLSDDQRNQLIKDLKIKANVLSERDFFQEVKELSKQF
                     PNDFKTLLIMINMVLSNLTNLNDSEMKEWSLSTLNKIISKTVVESDLQTAIMFKTVVL
                     FHNLEYDEVINLYQNSPYKLGEELLLANSFFAKGDVYRAKQVLQVEIYQQILLVCEYL
                     LSLSTFEVEENKGDIFERLEHMIEAFQLNSLHPNTVIKCYYSLACHYSKSDDDKALGY
                     LKQCFKSLKQLLQRFELHGDTFFYTIDDWLETIPTGIAPPTSQLQVIERVEDLFQNSQ
                     FKELSGRKEFQQIIQKLNDLKKDY"
     gene            1290..1496
                     /locus_tag="VBC94_001916"
     CDS             1290..1496
                     /locus_tag="VBC94_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEO6980153.1"
                     /translation="MNTEIPNQLLPFLVPLIMLQGALIIISLVKLSKLKMTKHLSKPV
                     WFLVIIFLNIIGPIAFLILEGNNE"
     gene            1493..2395
                     /locus_tag="VBC94_001917"
     CDS             1493..2395
                     /locus_tag="VBC94_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6980154.1"
                     /translation="MKAISFQNVTKSFGPKKILNNVSFDLEENMIYGFVGPNGAGKTT
                     TIKMILGLLKFDTGFITIFGKKVNFGRTDTNQLIGYLPDVPEYYDYMTALEYLDLCSG
                     LARSKHKLSNKELLRSVGLDDNHQKIATYSRGMKQRLGLAQALVHDPKIIICDEPTSA
                     LDPKGRQDILDIISNLRGEKTVIFSTHILSDVEKICDHVLVLTKCGIYSLEELKGKKS
                     EENYSVRILIKVTKSEAKVLSHNYQIEKKDNEYALTLKGSKMDNKADLLAGFYQDLVS
                     LKISPSAIEVIDNSLEELYLEVTK"
     gene            2392..3153
                     /locus_tag="VBC94_001918"
     CDS             2392..3153
                     /locus_tag="VBC94_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6980155.1"
                     /translation="MKSLAVMLKKEWMENVRTYKVISILITCSIFGILGPLTALMMPD
                     IMAGILPKKLQGAIPETTYIDSYIQYFKNMNQLGLVILVFLFSSTLTQEFSKGTLINL
                     VTKGLAKKVIILAKFIVITLLWTVSYLLSVVIHFSYTLYYFSNEGSHKLMVYGATWFI
                     GILFISLILFFSVLFRKTLGILLGCLFMVVLCVVSGFFKISKSYNPMLLIQKQGEMLS
                     GKFKVQDLMEPSILVFLLIIMTLLLSMYIFEKSEV"
     gene            3322..>3756
                     /locus_tag="VBC94_001919"
     CDS             3322..>3756
                     /locus_tag="VBC94_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6980156.1"
                     /translation="MEIKEYENNPYHLAQLVDLINYCQNIEAKLDIKMAEQDDIFQIE
                     NYYQNRKGQFWIALENEKVVGSIALLRIDDKTAVLKKFFTYPKYRGNPVRLGRKLFER
                     FMLFARASKFTRIVLDTPEKEKRSHFFYENQGFKQITRDKLDV"
BASE COUNT         1335 a          515 c          633 g         1273 t
ORIGIN      
        1 ttaagggctc ttttataatt cattattttt caatgacaaa tattagtgaa gttaattttt
       61 atttaaccat tgttattatt aatgctgttt tgccacttat taatcaacga aaaagtttct
      121 tctatggaaa cttttttata ttatatttcc tgataggaac ttttattttt gatttagaaa
      181 ctctataatg gacgtgaaag gagattgacc gatgcttgga gagaatatct atttacaacg
      241 tactcagatt ggtatgacgc aagagaattt gagtgattat ttgcatttaa caaaaactac
      301 aatttcaaag tgggaaaata atcaagctaa accggatatt gactatctta ttttgatggc
      361 taatcttttt gatattagct tggatgactt agtcggttat caaaaaacat tgtcagatga
      421 tcaaagaaat cagctcatta aggatctaaa aataaaagca aatgtattgt ctgagagaga
      481 tttctttcag gaagttaagg aattatcaaa acagttccct aacgatttta agactctgtt
      541 gataatgatt aatatggtct tgagcaacct aaccaacctt aatgattcag aaatgaaaga
      601 gtggtcacta agtacactaa ataaaattat tagtaaaaca gtagttgagt ctgacctcca
      661 gacggcgata atgttcaaaa cggtcgttct ttttcacaac cttgaatatg atgaagtgat
      721 aaacctttac cagaatagtc cctataaatt aggtgaagaa ttacttttag ctaatagctt
      781 ttttgcaaaa ggcgatgttt accgagctaa gcaagtatta caggttgaaa tttatcagca
      841 gattctactt gtatgcgagt atctattgag tttatcaact tttgaagttg aagaaaacaa
      901 aggcgatatt tttgaaaggc ttgaacatat gatagaagcg tttcaattaa actctttgca
      961 tcccaacaca gtcatcaagt gttattatag tttagcttgt cattatagta aaagtgatga
     1021 tgataaggca ttaggatatt taaaacaatg ttttaaatct ttaaaacagt tgctccaaag
     1081 atttgaatta cacggtgata ctttctttta taccatcgat gattggttag agacaatacc
     1141 tactggtatt gcaccaccga cgagtcaatt acaggtaata gaacgagttg aggacttatt
     1201 tcaaaattca caatttaaag aactatcagg gcgtaaagaa tttcaacaaa ttattcaaaa
     1261 acttaacgat ttaaaaaagg attactaata tgaatacaga aataccaaat caattattac
     1321 cattcctagt tccgttgatt atgttgcaag gagctttaat tatcatttct ctagtcaaac
     1381 taagtaaact taagatgact aaacaccttt caaagccagt ttggtttttg gtcattatat
     1441 ttcttaatat aattgggcca attgcatttc ttattttgga agggaataat gaatgaaagc
     1501 tatctcattt caaaacgtta caaaatcttt tggtcctaaa aaaatattaa ataatgtttc
     1561 ttttgattta gaagaaaata tgatttatgg attcgtagga ccaaatggag caggtaaaac
     1621 aacgacaata aaaatgattt taggattgtt aaaatttgat acgggtttca taacaatttt
     1681 tggtaaaaaa gttaattttg gaagaacaga taccaatcaa ctgattggct atctcccaga
     1741 tgttcccgag tactacgatt atatgacagc tctagaatat cttgatttat gttctggttt
     1801 agcgagatca aagcataagc tttctaacaa agaactactt aggtcagttg gacttgacga
     1861 taatcaccaa aagattgcta cttattctcg aggaatgaaa caacgtcttg gattagccca
     1921 agcactggtc catgatccta aaattattat ttgcgatgaa ccaacctctg cattagaccc
     1981 gaaaggtcgc caagatattt tagatattat ttcaaatctt agaggagaaa aaactgtgat
     2041 tttttctacc catattctct ctgatgtcga aaaaatatgt gatcacgttc tggtgttaac
     2101 taagtgtggg atttattctc ttgaagagtt aaaaggaaaa aaatcagagg aaaattattc
     2161 ggttcgtatt ttaataaaag tgacaaagtc tgaagctaag gtactatctc ataactatca
     2221 gatagaaaag aaagataatg agtatgcact gactctcaaa ggctctaaaa tggataacaa
     2281 agcagattta ctagctggtt tttaccaaga tttagtttct ttaaagatta gcccatcagc
     2341 tattgaagtc attgacaata gtttagagga gctttatttg gaggttacca aatgaaatct
     2401 ttagcagtaa tgttaaaaaa agaatggatg gagaatgtta ggacatacaa agttatatct
     2461 attctgatta cttgttcaat atttggtata ttaggtccct taactgcact tatgatgcct
     2521 gatattatgg caggaatttt acctaaaaaa ctacagggag cgataccaga gactacctat
     2581 attgattctt atattcagta ttttaaaaat atgaatcagc ttggtttagt tattttagta
     2641 tttttattta gtagtacact gacgcaagaa ttttcaaaag gaaccttaat taatctagtt
     2701 acgaagggat tagccaaaaa agttataatc cttgctaaat ttatagtaat tacccttcta
     2761 tggacagtgt catacttatt aagtgttgtc atccatttta gttatacgtt atattacttt
     2821 agtaacgaag gaagtcataa attaatggtt tacggtgcaa catggtttat cggtattctt
     2881 ttcataagct taatactctt tttttcagtt ctttttcgta aaactttagg gattttgtta
     2941 ggttgtcttt ttatggtggt gttatgtgta gtatctggat tttttaaaat cagtaaaagc
     3001 tataatccta tgcttcttat ccaaaaacaa ggagaaatgt taagtggaaa gtttaaagtt
     3061 caagacttaa tggaaccgag catattagtg tttttgctta ttataatgac gttattactt
     3121 agcatgtaca tttttgaaaa aagtgaagtt taattttgat aagatatcaa taaggcgata
     3181 gctacttttt gaaaaaataa aaccgaatta agagctttgc tgctatttga cttatctaga
     3241 tcaatatttc caactaatat gatatcctat gcatatctca ttgcagaaat atactttaaa
     3301 gtcaaaataa ggaaatatat tatggaaatt aaagaatacg agaataatcc gtaccattta
     3361 gcacagctag tagatttaat taattattgt cagaatatag aagctaaact tgatataaag
     3421 atggctgaac aagacgatat ttttcaaata gaaaattatt atcaaaacag aaaaggtcaa
     3481 ttttggattg cattagaaaa tgaaaaagta gttggttcga ttgcgttact gcgtattgat
     3541 gataaaacag ctgttctaaa aaaatttttt acatatccaa aatatagagg aaatcctgtt
     3601 cgactcggtc gaaaattatt tgaaagattt atgctttttg ctcgagctag taaattcaca
     3661 agaattgtct tggatactcc agaaaaagag aaaagatctc acttctttta tgaaaatcaa
     3721 ggatttaagc aaataacaag agataaacta gatgta
//
LOCUS       DATROQ010000131         3712 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.132, whole genome shotgun sequence.
ACCESSION   DATROQ010000131 DATROQ010000000
VERSION     DATROQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.132"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            303..1124
                     /locus_tag="VBC94_001920"
     CDS             303..1124
                     /locus_tag="VBC94_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6980157.1"
                     /translation="MIKLIATDMDGTFLRSDKTYDKARFSSLLTLMEKYDIKFVAASG
                     NLYDQLLLNFLEYPNRIAYVAENGGRVIDQDGTLLKETYLSNDTVAAVLSYLYQNYPE
                     TLISLSGEKRSYLERRTPINRRTELEYYMPNFIYKDHLLPLDDDRYFQMTLWVNENLV
                     SEMLLDISEHFKNHHIRLTSSGFGCIDVLPADVNKADGIAILLEKWGLKQDQVMVFGD
                     GGNDVEMLRAANISYAMSNAPEEIKAIAKYQTVSNDQDGVLETIENFLHKMSDNE"
     gene            1225..1839
                     /locus_tag="VBC94_001921"
     CDS             1225..1839
                     /locus_tag="VBC94_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEO6980158.1"
                     /translation="MLEVIDKALRDYQMKREQFFEINNQTVQEGAIVFTGDSIVEFFP
                     LKKHLGRDYPLVNRGVAGSDSYWLLENLRTQVWELLPSKVFILIGTNDIGLGHSQSEI
                     IANITDIIAEIRAESYMTEINILSVLPVSEEDDYIERVKVRNNQAIKALNKTLSVISG
                     INYIELYDLLVDEKGQLASSFTKDGLHLTDQAYAKISETIKLHL"
     gene            1999..3438
                     /locus_tag="VBC94_001922"
     CDS             1999..3438
                     /locus_tag="VBC94_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEO6980159.1"
                     /translation="MMVKQVFPKGFLWGGATAANQCEGAYNVDGRGLANVDVVPTGED
                     RFAIISGQKKMFDFEEGYFYPAKESIDFYHHYKEDLALLAEMGFKTYRMSIAWTRIFP
                     KGDELYPNEAGLRFYENIFKECRKYGIEPLVTITHFDCPIYLIKHYGGWRSRKMIGFY
                     ERLVRALFTRFKGLVKYWLTFNEINMILHAPFMGAGLYFEDGENQEQIKYQAAHHELV
                     ASAIAVKIAHEVDPNSQIGCMLAAGQYYPNTCHPQDYWASMQKNRENYFFIDVQARGK
                     YPNYAKKYFEHLGISIQMTAEDLALLRDYTVDFISFSYYSSRVASGNPTVSEQVQENI
                     FASLKNPYLKSSEWGWQIDPLGLRITLNAIWDRYQKPMFIVENGLGAVDIPDENGYVE
                     DDYRIDYLRQHIAAMRDAIYVDGVNLIGYTTWGCIDLVSAGTGEMEKRYGLIYVDRNN
                     KGEGTLKRYKKKSFYWYKKVIASNGSQIE"
     gene            3529..>3712
                     /locus_tag="VBC94_001923"
     CDS             3529..>3712
                     /locus_tag="VBC94_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO6980160.1"
                     /translation="MKFEKRQVYYVVITFAICYAIQAYWGAVSNILTTLHKAIFPFLM
                     GAGIAYIINIVMSVYER"
BASE COUNT         1248 a          529 c          725 g         1210 t
ORIGIN      
        1 ctctcaaaaa cacgtatagc aatttcgaat ctcaaatatt ttattatgac tattgtaata
       61 gttacgaatc tattattcaa ctcaacaata agaaaactct tcagtaaatc attatgttaa
      121 acaagctctg atagtatgga gataagtata ggtaaaagtc gatttaatct ctgaaacaaa
      181 atttaactta attaatagaa ccgttgacgt atcaggtatc aaatcttttt ggtttatatg
      241 tgatataatg aaagaacata ttgagatatt tggcaccctc ttgaaaggca aaggttactt
      301 gtatgattaa actaatagca acagatatgg atggaacttt tctacgctca gataaaactt
      361 atgataaagc acggttttct tctttattaa cgttgatgga gaagtatgat attaaatttg
      421 tagctgctag tggtaattta tacgatcaat tgttgttaaa ctttttggaa taccctaatc
      481 gtatcgccta cgtcgctgaa aatggtggtc gtgtcattga ccaagatggc acattactaa
      541 aagaaactta tttatctaat gatacagttg cagctgtttt atcctattta tatcagaact
      601 atcctgagac actcatcagc ttatctggtg aaaaacgttc ataccttgag cgaagaactc
      661 ctattaatag gcggactgaa ttagaatact atatgcctaa ttttatctat aaagaccatt
      721 tgctgccctt agatgatgac cgatattttc aaatgacact ttgggtaaat gaaaatcttg
      781 tttctgagat gttgttagat attagtgaac actttaaaaa tcatcatatc agattgacct
      841 ctagtggttt tggttgtatc gatgtcttac cagctgatgt caataaagcg gatggtatcg
      901 ctattctcct tgaaaagtgg ggattgaaac aggatcaagt gatggttttt ggtgatgggg
      961 gcaatgatgt ggaaatgttg agagcagcaa atatttctta cgctatgtca aatgcgccag
     1021 aagaaattaa agctattgct aaatatcaaa cagttagtaa cgatcaagat ggtgtgttag
     1081 aaacgataga gaactttctt cacaagatga gtgataatga atagaaccac gtagcttttc
     1141 acgaagcata ctattcaatt ctcaggtgaa attttgttat aatacaagaa agcctagaga
     1201 aatatttaag aatgaaggag aacgatgtta gaagttattg acaaagcttt aagagattat
     1261 caaatgaaac gagaacagtt ttttgagatt aacaatcaga cagtacaaga aggtgccatt
     1321 gttttcacag gagattctat cgttgagttt tttcctttaa aaaaacattt aggacgtgac
     1381 tatcctttag ttaatagagg agtagcagga tcagattctt attggctttt agaaaacttg
     1441 agaacccaag tatgggaact attaccatcc aaagtattta ttttaattgg aacaaatgat
     1501 attggtttag ggcattcaca gtcagaaatc atagctaata tcacggatat aattgcagaa
     1561 attcgtgcgg aatcttatat gacagagatt aatatactga gtgttttacc tgttagtgaa
     1621 gaagatgact atatagagcg tgtcaaggta agaaataatc aagcaataaa agctttaaac
     1681 aaaaccttat cagtaatttc tggcattaat tatattgaat tgtatgattt attagttgac
     1741 gaaaaaggtc agcttgctag cagttttaca aaagatggtt tgcacctaac agatcaagcc
     1801 tatgctaaaa tttctgaaac gataaaactc cacctttaaa agtgtttaga tactattttt
     1861 aagtatttaa atacacagaa ttagtgaaaa gaccttctaa gtagaaagtt catgtcacgg
     1921 atagtaccag tatttggcga atcaatgtca gtaactggtc ttttttaata ttatgataag
     1981 aaaaaacaag aggggtaaat gatggtaaaa caagtatttc caaaaggttt tttatggggc
     2041 ggggcaactg ctgccaatca atgtgaagga gcttacaatg ttgatggacg tggcctagca
     2101 aatgtagatg ttgttcctac tggagaagat cgatttgcaa ttatttcagg acaaaagaaa
     2161 atgtttgatt ttgaggaagg atacttttat ccagcaaaag aatcaataga tttttatcac
     2221 cattataaag aagaccttgc attacttgca gaaatgggtt tcaaaaccta tcgtatgtca
     2281 atagcatgga cgcgtatttt tccaaagggt gatgagctat atccaaatga agctggtctt
     2341 cggttttatg aaaatatttt taaagagtgt cgtaagtatg gtattgaacc tttggtaacc
     2401 atcacacatt ttgactgtcc tatctacctt attaaacatt acggtgggtg gcggagccgt
     2461 aaaatgattg gtttttatga gcgtcttgta cgagctttat ttactcgttt taaggggtta
     2521 gttaaatatt ggttgacctt taacgaaatc aatatgattt tacacgctcc ttttatgggc
     2581 gctggcttat attttgaaga tggtgaaaat caagagcaaa ttaaatatca agcagctcac
     2641 catgagttag tggcttcggc tattgcagta aaaattgctc atgaagttga tccaaatagt
     2701 caaatcggat gtatgttagc tgcaggtcaa tactatccaa atacgtgtca tccacaagat
     2761 tattgggctt cgatgcaaaa aaatagagaa aattattttt ttattgatgt gcaagcgcgt
     2821 ggcaaatacc ctaattatgc taaaaaatat tttgagcatt taggtatttc aattcaaatg
     2881 acagcagaag atcttgcttt gttaagagac tatacggttg attttatttc attttcttac
     2941 tattcaagcc gagtagcttc aggtaatcca actgtcagtg aacaggttca agaaaatatt
     3001 ttcgcatctc ttaagaatcc ttacttgaaa tcttctgaat ggggatggca aattgatccg
     3061 cttggattac gcattacctt aaatgctatc tgggatcgtt atcaaaaacc gatgtttatt
     3121 gttgaaaatg gacttggagc agtagatatt cctgatgaga atggttacgt agaagatgat
     3181 tatcgtattg attacctccg tcagcacatt gctgcaatga gagatgctat atatgtagat
     3241 ggtgttaatc taattggcta tactacatgg ggatgtattg atttagtatc tgcaggaact
     3301 ggtgaaatgg aaaaacgcta tggtttaatt tatgttgatc gcaataataa aggagaggga
     3361 acactcaagc gttacaagaa aaaatcgttt tattggtaca agaaggttat tgcaagtaat
     3421 ggcagtcaaa tagaataaag agcagactaa taactgttaa ctctcttttt ctcattcata
     3481 aaatagtgta taataattga aagaaattaa gattaataag gagatttcat gaaatttgaa
     3541 aaacggcagg tctattatgt tgtcataaca tttgctattt gctatgctat acaggcttat
     3601 tggggagctg tttctaatat tttaactacg cttcataagg caatatttcc ttttttgatg
     3661 ggagctggaa ttgcctatat tattaatatt gtaatgtcag tctatgagcg at
//
LOCUS       DATROQ010000132         3638 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.133, whole genome shotgun sequence.
ACCESSION   DATROQ010000132 DATROQ010000000
VERSION     DATROQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.133"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            56..3475
                     /gene="srr2"
                     /locus_tag="VBC94_001924"
     CDS             56..3475
                     /gene="srr2"
                     /locus_tag="VBC94_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086874280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin Srr2"
                     /protein_id="HEO6980161.1"
                     /translation="MLKKQFGNFGEKSRKVRVKMRKSGKHWVKSVMTQIGYVILSRFS
                     GKEKSSKVQTTSEDLSRTKTSASILTAVAALGAVVGGTTDTTSVSAEETPTATELTGN
                     EKTLATAETVVVAPEVKTVNSDSSSHSTSESQSMSTSTLQSTSASLSASESLMDSTSA
                     SLSESSSLSEYSSLSLSSSESVSASESVQSSEAATTARVQPRAMRVVSSASDMETLPA
                     ALISGEGDVTTVQGQDVTDKLQNLDIKLSGGVQAKAGVINMDKSESMHMSLKFTIDSV
                     NRGDTFEIKLSDNIDTNGASNYSIVEPIKSPTGEVYATGIYDSQKKSIVYSFTDFAAS
                     KNNINGILDIPLWPDDTTVQNTKEDVLFSVKIKDQEATIKETVKYDPPVRIDFAGGVS
                     VDSRITNIDDVGKKMTYISQINVDGKSLYNYNGLYTRIYNYSKESTADLKNSTIKIYK
                     TTSDNIVESMVQDYSSMEDVTSKFANSYPEKGWYDIYWGQFIASNETYVIVVETPFTN
                     AVTLNTTLSDYNENNGVEHNHTYSSESGYSDVNAQERKILSELVSSSESVSSSESVSN
                     SESISTSESVSNSESISSSESVSSSESISTSESVSTSESISSSESVSSSESVSSSESI
                     SSSESVSNSESISSSESVSNSESISSSESVSSSESISNSESISSSESVSTSESISSSE
                     SVSNSESISSSESVSSSESISNSESISSSESVSTSESISSSESVSNSESISTSESVST
                     SESISSSESVSSSESISSSESVSNSESISNSESVSSSESVSNSESISSSESVSNSESI
                     STSESVSTSESISSSESVSNSESISSSESVSNSESISSSESVSNSESISSSESVSTSE
                     SISSSESVSSSESVSSSESISTSESVSNSESISSSKSVSNSESISSSESVSNSESISS
                     SESVSNSESISSSESVSSSESISSSESVSSSESVSTSESISSSESVSNSESISSSESV
                     STSESISSSESVSNSESISSSESVSSSESVSSSESVSSSESISTSESVSNSESISSSK
                     SVSNSESISSSESVSNSESISSSESVSMSTTESLSESEVSGDSEISSSTESSSQSESM
                     NHTEIKSDSESQHEVKHQVLPETGDNSASALGLLGAGLLLGATKSRKKKKD"
BASE COUNT         1224 a          651 c          661 g         1102 t
ORIGIN      
        1 tcttagaatt agaaaagttt tttatatagc attatttttt aaatatggag gaaaaatgtt
       61 aaaaaagcag tttggaaact ttggtgaaaa atcacgcaaa gttcgagtta aaatgaggaa
      121 atcaggtaag cactgggtca aaagtgttat gacacaaatt ggctatgtta tcctttcgag
      181 gtttagcggt aaagagaaaa gctctaaggt tcaaactact agtgaagatt taagcagaac
      241 taaaacgtct gctagcatcc taacagcagt cgcagctctt ggagctgtag ttggagggac
      301 gacagacaca acttctgtct cagctgaaga aacacctact gcaacagaac ttacaggaaa
      361 tgaaaaaaca ttagctactg ctgaaactgt agtggttgcg ccagaagtaa aaactgtaaa
      421 ctcagattct tcatcacatt caacaagtga atcacaatca atgtcaacta gtaccttgca
      481 atctaccagc gcctcattat ctgccagtga atcactaatg gattctacct ctgcttcttt
      541 atctgagtct agctcgttat cagaatatag ttctttatct ctatctagta gtgagagtgt
      601 ctcagcatca gaatcagttc aatcatcaga agcggcaacg accgctagag ttcaaccgag
      661 agcgatgaga gttgtatcta gtgcttcaga tatggaaact ttaccagcag cattaatctc
      721 tggtgaagga gatgtaacaa ctgttcaagg acaagatgta acagataagt tacaaaattt
      781 agatataaag ctctctggag gagtgcaagc aaaagcaggt gttataaata tggataagtc
      841 agaaagtatg cacatgtctt taaaattcac tattgattct gtgaatagag gtgatacttt
      901 tgaaattaag ttatcagata atatcgatac aaatggagct tctaactatt ctattgtaga
      961 acctataaaa tcacctacgg gtgaggtcta tgcgactgga atttatgatt ctcaaaaaaa
     1021 atctatagtc tatagtttta cagattttgc ggcttcaaaa aataatatta atggaatatt
     1081 agatattcca ttatggccag atgatacgac agttcaaaac acaaaagaag atgttctttt
     1141 ttcggtaaaa ataaaggatc aagaggctac aattaaagaa acagtgaaat atgatccacc
     1201 ggtaagaatt gattttgcag ggggagtaag tgtagattct cgaataacca acattgatga
     1261 tgtgggaaaa aagatgactt atataagtca aattaatgta gatggaaaat cactctataa
     1321 ttacaacggg ttatatacta ggatatataa ttatagcaaa gagagtacag cagatttaaa
     1381 gaattcaacg ataaaaatct ataaaaccac ctcggataat attgtagaga gtatggtaca
     1441 ggattattca agcatggagg atgtaactag taagtttgca aatagttacc cagaaaaagg
     1501 gtggtatgat atttattggg ggcagtttat tgcatcaaat gaaacgtatg taattgttgt
     1561 agaaacacca tttactaatg cagtaacttt gaatactact ttatcagatt ataatgagaa
     1621 caatggtgta gaacataatc atacttactc atctgaatcg ggatattcag atgtaaatgc
     1681 tcaagaaaga aaaattttat ccgaattagt aagtagctca gaatcagtat caagttcaga
     1741 atcggtatct aattccgaat ctatttcaac ttctgaatcg gtatctaact ccgagtctat
     1801 ttcaagttcc gaatcggtat caagctcaga atctatttca acttctgaat cggtatcaac
     1861 ttcagagtct atttcaagct ccgagtcagt atcaagttca gaatcggtat caagctcaga
     1921 atctatttca agttccgaat cggtatctaa ctccgagtct atttcaagct ccgaatcggt
     1981 atctaactcc gaatccattt caagttctga atcggtatca agctcagaat ctatttcgaa
     2041 ttcagagtct atttcaagct ccgagtcagt atcaacttca gagtctattt caagctccga
     2101 atcggtatct aactccgaat ccatttcaag ttctgaatcg gtatcaagct cagaatctat
     2161 ttcgaattca gagtctattt caagctccga gtcagtatca acttcagagt ctatttcaag
     2221 ttccgaatcg gtatctaact ccgaatccat ttcaacttcc gagtcagtat ctacctcgga
     2281 atctatttca agttctgaat cggtatcaag ctcagaatct atttcaagtt ccgaatcggt
     2341 atcaaactcc gagtctattt caaactccga gtcggtatct agctctgagt cagtatcaaa
     2401 ttcagagtct atttcaagtt ccgaatcggt atctaactcc gaatccattt caacttccga
     2461 gtcagtatct acctcggaat ctatttcaag ttctgaatcg gtatcaaatt cagagtctat
     2521 ttcaagttcc gaatcggtat ctaactccga gtctatttca agttcagaat cggtatcaaa
     2581 ttcagagtct atttcaagtt ccgaatcggt atcaacttca gagtctattt caagttcaga
     2641 gtcagtatca agttcagagt cagtatcaag ctcggaatct atttcaactt cagaatcggt
     2701 atctaactcc gagtctattt caagttccaa atcggtatct aattctgaat ctatttcaag
     2761 ttccgaatcg gtatcaaatt cagagtctat ttcaagttcc gaatcggtat caaactccga
     2821 gtctatttca agttccgagt cagtatcaag ctcagaatct atttcaagtt ccgaatcggt
     2881 atctagctcc gagtcagtat caacttcaga gtctatttca agttccgaat cggtatctaa
     2941 ctccgagtct atttcaagtt ccgaatcggt atcaacttca gagtctattt caagttcaga
     3001 atcggtatca aattcagagt ctatttcaag ttccgagtca gtatcaagtt cagagtcagt
     3061 atcaagttca gagtcagtat caagctcgga atctatttca acttcagaat cggtatctaa
     3121 ctccgagtct atttcaagtt ccaaatcggt atctaattct gaatctattt caagttcaga
     3181 atcggtatca aattcagagt ctatttcaag ttcagagtca gtatcaatga gtactacaga
     3241 gtctctaagt gaatcagaag tatcagggga ttctgaaatt agctcaagta cagaatcatc
     3301 aagtcaatct gaatcgatga atcatactga aattaaatcc gattccgaat ctcaacacga
     3361 agttaagcat caagtattac cagaaacagg tgataactcg gcttcagcat taggtctgtt
     3421 aggtgcagga ttgttgttag gagctactaa atctcgcaag aagaaaaaag attaattttc
     3481 aaagtgatat ttagatgata aatcgaaagt actttcgatt tatcacttaa gatagtaagt
     3541 agttatactg tcttaagtga taaattgatt tgaaaaggga aaaaatgatt ggaaaattat
     3601 attatagcta tagaaagtca acgctattaa gaagtatt
//
LOCUS       DATROQ010000133         3512 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.134, whole genome shotgun sequence.
ACCESSION   DATROQ010000133 DATROQ010000000
VERSION     DATROQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.134"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            25..2184
                     /locus_tag="VBC94_001925"
     CDS             25..2184
                     /locus_tag="VBC94_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980162.1"
                     /translation="MKKYLSIVALLTILFSSIYYLYYFDGSLYLPKGLLKENTRTNFV
                     VKGDTVLHKPTNKPFVVKGVDVESSLAGYHHNDFPITQKTYREWFHLISNMGANTVRV
                     KVPMNVAFYDALYHHNKESKRPLYLLQGIRIDSYRNNASITAFNDNYRGYLKREAKGV
                     VDILHGRKQVWNTDFGSRHYHYDLSPWVLGYVVGDDWNSGTVAYTNHQEKKTQYKGRY
                     FKTSVAANPFEVMLAQVMDELTHYETAKYGWQHLISFSNSPTTDPFHYRKPFEAQAPK
                     YVQLNVENIQANSNVKAGMFAAYKAIDFHPRYKDYLLFDKENISKEDRQKIKELSLSQ
                     GYVKLLNAYHKIPVLVTGYGYSTARGIAQKEIDKRPLPINEKEQGQRLLEDYESFISS
                     GSFGATINAWQDDWNARAWNTSFATNKHSQFLWGDAQVFNQGYGLLGFKNAKHHYQVD
                     GKRGKGEWKHPLMTSATGDDLYASSDESYLYLAIKTKPEKLKEKRLLPIDITPKSGSR
                     KMNGSKVTFSKSSDFVLSIDPNGKSELFVQERYNALKANYLRQLNGKDFYAFPPKKNS
                     SNFEQINMVLRNTKIVEDMEKVKATERFLPTHPTGLLKTGTTDRHQKTFDSQTDISFG
                     KDFIEVRIPWQLLNFSDPSSQKIHDDYFKHYGVKELEIESIALGLGANSKENTLIKMA
                     DYRLKNWERPDTKTFLKDSYYSIKKEWSKERERTYGT"
     gene            2324..3512
                     /locus_tag="VBC94_001926"
                     /pseudo
     CDS             2324..3512
                     /locus_tag="VBC94_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643060.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT         1176 a          530 c          673 g         1133 t
ORIGIN      
        1 aatactttac tggagtagaa cattatgaaa aaatatctaa gtattgtagc ccttttaact
       61 attttgttta gctctattta ttatttgtat tactttgatg gtagtttgta tttaccaaag
      121 ggcttattaa aagaaaatac aagaactaac tttgttgtta aaggtgatac tgtacttcac
      181 aagcccacca ataaaccttt tgttgttaaa ggagtagacg ttgagtcttc cttagcgggt
      241 tatcatcaca acgattttcc tattactcaa aaaacgtatc gtgaatggtt ccatttaatt
      301 tccaacatgg gggcaaatac tgtaagagtc aaggtaccga tgaatgttgc attttacgat
      361 gccttatatc accacaacaa agaatcaaag aggccactgt atttgttgca aggaatacgt
      421 atagattctt atcgcaataa tgcttctata acagctttta atgataatta tagggggtat
      481 ttaaaacgag aagcaaaagg cgttgtggat attctccatg ggcgtaagca agtatggaat
      541 actgattttg gtagccgtca ttatcattat gatcttagtc cttgggtact tggttatgtc
      601 gtaggggatg attggaatag tggtactgtc gcttatacta atcatcaaga gaaaaaaacg
      661 caatataaag gacgttattt taaaacttct gtggcagcta atccatttga ggtcatgcta
      721 gctcaagtaa tggatgaatt gacacattat gagacagcta aatatggttg gcaacatttg
      781 attagttttt caaactcacc aacaacagac ccttttcatt atcgaaaacc atttgaggca
      841 caggctccta aatacgtaca actaaatgta gaaaatattc aagctaattc aaatgttaaa
      901 gcaggtatgt ttgcagcata taaagctatt gatttccatc ctcgatacaa ggattatcta
      961 ttatttgata aagagaatat cagtaaagaa gatagacaaa agattaaaga actttctttg
     1021 tcacagggat acgttaaact gctaaatgct tatcacaaaa tccctgttct agtcacgggt
     1081 tatggctatt cgacagcgag aggtattgcc caaaaagaaa ttgataaacg tcctctgccg
     1141 attaatgaaa aagaacaagg tcagcgttta ctagaagatt atgaatcttt tatatcatcc
     1201 ggtagttttg gagcgactat caatgcatgg caagacgatt ggaatgcaag ggcgtggaat
     1261 acatctttcg ccacaaataa acatagtcaa ttcctatggg gggatgcaca agtatttaat
     1321 caaggttatg gtttattagg ctttaaaaac gcaaaacatc attatcaagt tgatggtaaa
     1381 agaggcaaag gagagtggaa acatcctctg atgactagtg caacaggaga tgacttatat
     1441 gctagcagtg atgaaagcta tctctacctt gcgattaaaa caaaacctga aaaactaaaa
     1501 gaaaaacgat tattaccaat agatattaca ccaaaatctg gtagtagaaa aatgaatggt
     1561 agtaaggtca cattttctaa atctagtgac tttgtattgt ctattgatcc aaatggcaag
     1621 tctgaattat ttgtccaaga gcgctataat gccttaaaag cgaactatct tcgacagctt
     1681 aacggtaaag atttttatgc tttcccacca aagaagaaca gtagtaattt tgagcagata
     1741 aatatggtat tgagaaatac aaagattgtt gaagacatgg aaaaagtaaa agcaacagag
     1801 aggttcttac caactcatcc tactggtctt ctcaaaacag gaacaactga taggcaccaa
     1861 aaaacatttg attcacaaac agatatttcg tttggaaagg actttataga ggtcagaatt
     1921 ccgtggcagt tgttgaattt ttctgatcca tcatctcaaa aaattcacga tgattacttt
     1981 aaacattatg gtgtgaagga gttagaaatt gagagcattg ctttaggatt aggtgctaat
     2041 agcaaagaaa acacactgat aaagatggca gattatcgtt tgaaaaattg ggagagaccc
     2101 gataccaaaa cctttttaaa agactcctat tatagtatta agaaagaatg gtctaaagaa
     2161 agagagagaa catatggtac ataatatttt atatttttac attttgattt gcttagcaat
     2221 tattgttttt aacattcact atatcttaag acaacgatta gataagctta gttggcttcg
     2281 tattcggaaa aaggatataa atatcaaata aaggggattt tctatggcat tattttttac
     2341 cttcctaaag gtatctcaag tcattttctt agtattattg attgcctatg ccagcttttt
     2401 agtgatttca ttagtcattg gaggtgttca tctctatgaa aaaaatgagc gcaaaaaaag
     2461 tttaactaga aataaagaaa aaattccaat ttcaattatt gtaccagcct ataatgaatc
     2521 aacaaccatt gtgagttcga ttgattcgtt attgcacctt gattatgaag cgtatgaaat
     2581 tatcgttgtg gatgacggtt ccagtgacaa tacttcagat gttctcaagg aagagtttgc
     2641 attaatgaaa atctcgaata cgattgattc aattattgct actcaaactt gtaaagatgt
     2701 ctttcagaga caggttggta aggttaaact gacactcatt gttaaagaaa atggtggcaa
     2761 aggggatgcc ttaaatatgg ggatcaatgc tgctaattac gattattttc tatgtctaga
     2821 tgcagattct atgttgcagg tagattcttt gagtcaaatt agcaaatcca tacaggtaga
     2881 tccgacggtt atagcagtgg gagggcttgt ccaagttgcc caaggtgtta agattgagca
     2941 ggggaaagta gcatcttatc gtttgccttg gagaattatt ccttgtgcac aagcacttga
     3001 gtatgattca tcatttttag gagctcgaat ctttttagac tatttaagag ctaatttaat
     3061 tatttcgggg gctttcggtc tttttaagaa agaccttgtt aaagcggtgg gaggatatga
     3121 cacccagact ctaggagaag atatggagtt ggtgatgaaa ttacatttct tctgtcgtaa
     3181 taataatatt ccttatcgta tttgttatga aacagatgct gtttgttgga gtcaagcacc
     3241 aacgaattta ggggatttac gtaaacagag acgccgatgg tatcttggct tgtactaatg
     3301 tttaaaaaaa tataaaagta tttttgccaa ttatcgcttt ggagctgtag gatcaatttc
     3361 ttatatttac tatattttat ttgaattact gacccctttt atagaatgtt ttggtattgt
     3421 cattatattt ttgtccttat tatttaatca attaaacatc ccattcttta tatcattggt
     3481 gagcctttat atcttttact gtgttttaat ta
//
LOCUS       DATROQ010000134         3505 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.135, whole genome shotgun sequence.
ACCESSION   DATROQ010000134 DATROQ010000000
VERSION     DATROQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.135"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..710
                     /locus_tag="VBC94_001927"
     CDS             <1..710
                     /locus_tag="VBC94_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6980163.1"
                     /translation="RLKKINLLGNKFDVVKNLKEAYVPETTNTYNPGLIIFANNKQID
                     NIRKAYLPYTKNINTFPKTFKAYLDLNSQEINSISKNDIIEVDGKYVGNISTKQSFLK
                     EGYQMFGGLLFTGFLLGISFLLGIALIVYYKQYSEGHEDKRSYRILQEVGMSKKLVKR
                     TINSQIMIFFFQPLVVAVIHFGVAIPMLKQMLLVFGVLNSTIVYVVSGLTVLAISIIY
                     FIIYRITSRTYYHIIER"
     gene            820..1488
                     /locus_tag="VBC94_001928"
     CDS             820..1488
                     /locus_tag="VBC94_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6980164.1"
                     /translation="MSQEQGKIYIVEDDMTIVSLLKDHLSASYHVSSVSNFRDVKQEI
                     IAFQPDLILMDITLPYFNGFYWTAELRKFLTIPIIFISSSNDEMDMVMALNMGGDDFI
                     SKPFSLAVLDAKLTAILRRSQQFIQQELTFGGFTLTREGLLSSQDKEVILSPTENKIL
                     SILLMHPKQVVSKESLLEKLWENDSFIDQNTLNVNMTRLRKKIVPIGFDYIHTVRGVG
                     YLLQ"
     gene            1485..2423
                     /locus_tag="VBC94_001929"
     CDS             1485..2423
                     /locus_tag="VBC94_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6980165.1"
                     /translation="MIRQFLREHLIWYILYIMMFVLFFISFYLYHLPMPYLFNSLGLN
                     VIVLLGISIWQYSRYRKKMLHLKYFNSSQDPSFELQPSDYAYFNIITQLEAREAQKVS
                     ETIEQTNHVALMIKMWSHQMKVPLAAISLMAQTNHLDPKEVEQQLLKLQHYLETLLAF
                     LKFRQYRDDFRFEAVSLREVVVEIIKSYKVICLSKSLSIIIEGDNIWKTDKKWLTFAL
                     SQVLDNAIKYSNPESKIIISIGEESIRIQDYGIGILEEDIPRLFEDGFTGYNGHEHQK
                     ATGMGLYMTKEVLSSLNLSISVDSKINYGTAVSIHK"
     gene            complement(2466..>3505)
                     /gene="xerS"
                     /locus_tag="VBC94_001930"
     CDS             complement(2466..>3505)
                     /gene="xerS"
                     /locus_tag="VBC94_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEO6980166.1"
                     /translation="ELKEIMPWYVLEYYQSKLSVPYSFTTLYEYLKEYRRFLEWLLDS
                     GVANCHHIAEIELSVLENLTKKDMEAFILYLRERPLLNANTRQNGVSQTTINRTLSAL
                     SSLFKYLTEEVENADGEPYFYRNVMKKVSTKKKKETLASRAENIKQKLFLGNETIEFL
                     EYIDCEYQNKLSKRALAFFNKNKERDLAIIALLLASGVRLSEAVNLDLKDINLNVMVI
                     DVTRKGGKRDSVNVASFAKPYLANYLDIRKNRYKAENQDIALFLSEYRGVPNRIDSSS
                     VEKMVAKYSQDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHASTQVTDLYTHIV
                     NDEQKNALDKL"
BASE COUNT         1176 a          531 c          605 g         1193 t
ORIGIN      
        1 gccgtttgaa aaaaataaat ctattgggaa ataaatttga cgttgtaaaa aatttaaaag
       61 aggcttatgt cccagagact acaaatactt acaacccggg tttgattata tttgcaaaca
      121 acaaacaaat tgacaatatt agaaaagcat atttacctta tactaagaac atcaatactt
      181 ttccaaaaac ctttaaggca tatttggatt tgaatagtca agagataaat tcaatttcta
      241 aaaacgacat aatagaggta gatggtaagt atgttggaaa tattagcact aaacagagtt
      301 ttctaaaaga aggttatcaa atgtttggtg ggttattatt tactggattt ctgttaggta
      361 taagcttttt gctaggtata gctctaattg tttactataa gcaatactca gaaggtcatg
      421 aagataagag atcttatcgt atcttacaag aagtaggtat gagcaaaaaa ttagttaaac
      481 ggactattaa ttctcaaatt atgattttct tcttccaacc tttagtggta gcagttattc
      541 actttggagt tgctattcca atgctaaaac agatgctttt ggtatttggg gtgcttaact
      601 caacaatcgt atatgtagta agtggtttga cagtacttgc gatttctata atttacttta
      661 tcatttaccg tataactagt cgaacttatt atcatattat tgaacgctaa aaaggattgg
      721 ttattttacc aatccttttg cattaataga aaccgataga ctaaaaaatt tgttatacta
      781 aatgaaagaa agagatttcg tactatttta ggaggggata tgtcacaaga gcaaggaaaa
      841 atttatattg tagaagatga tatgacgatt gtgtcacttt taaaagatca tttatcagct
      901 agctatcatg tctctagtgt cagcaatttt cgtgatgtga aacaagaaat tatcgcattt
      961 caacccgatt tgatactaat ggatattacg ttaccctatt ttaatggttt ttactggact
     1021 gcagaattgc gtaagttttt aacaattcct attattttca tttcatctag taatgatgaa
     1081 atggatatgg ttatggcatt aaatatgggg ggtgatgact ttatttcaaa accattctct
     1141 ctagctgtat tagatgctaa gctaactgct attttaagga gaagtcaaca atttatccaa
     1201 caggaattaa cttttggggg atttacgttg acaagagaag ggttattgtc tagccaagat
     1261 aaagaggtta ttttatcgcc aacagaaaat aaaatcctat ctatcttgct catgcatcct
     1321 aaacaagtag tctcaaaaga gtctctatta gagaaacttt gggaaaatga tagttttatt
     1381 gatcaaaata cacttaatgt taatatgaca cgcttacgta aaaaaattgt cccaataggt
     1441 tttgattaca ttcatacagt gagaggagtt gggtatttac tacaatgata cgccagtttt
     1501 taagagaaca cttgatttgg tatattttat atatcatgat gtttgtccta ttttttatta
     1561 gtttctatct atatcattta ccaatgccct atttgtttaa ttccttaggt ttaaatgtta
     1621 ttgttttact aggaattagt atttggcaat acagtcgtta caggaaaaaa atgttacatc
     1681 tcaaatattt taatagtagt caggacccct ctttcgaact tcaaccgagt gattacgctt
     1741 attttaatat tattacacaa ttagaagcta gagaagcgca aaaagtttct gaaacaattg
     1801 aacaaaccaa tcatgttgca cttatgataa agatgtggtc gcaccaaatg aaagttccat
     1861 tggcagctat ttcattaatg gcccagacaa atcatctcga tcctaaggaa gttgaacaac
     1921 aattattgaa attgcaacat tatcttgaaa cgttgttagc atttttgaaa tttagacaat
     1981 atcgtgacga ttttcgtttt gaagctgtta gccttagaga agtagtagta gaaattataa
     2041 aatcgtataa ggttatttgt ctatccaaaa gcttatctat cataattgaa ggcgataata
     2101 tctggaaaac agacaaaaag tggttaactt ttgctctttc acaggtgcta gataatgcca
     2161 taaaatattc taatcctgag tcaaagataa taataagcat aggagaagag agtattagaa
     2221 tacaagacta cggtatcggc atactcgaag aggatatccc tagacttttt gaagatggct
     2281 ttacgggtta caacggtcat gagcaccaaa aggcaacagg catggggtta tatatgacaa
     2341 aagaagtctt atctagtctg aatttgtcca tttcggtgga tagcaaaatt aattatggga
     2401 ctgctgtttc tatacataaa taaagttatg taaaataaaa tatttacata actttattta
     2461 tgttactata gtttatctaa agcgttcttt tgttcatcgt taacaatatg ggtatatagg
     2521 tcagtaactt gagtggaagc atgacctagt tggtggctaa ctaatacttg agattttgta
     2581 gcatcatata gcctagtagc aagagtatgc cttagtttat gaggtgttac acgaacctta
     2641 aaatcctggg aatacttagc aaccattttc tctacacttg aagaatcaat acgatttggc
     2701 accccacggt attctgataa aaatagagca atgtcctgat tttctgcttt gtatctattt
     2761 ttacggatgt ctaaataatt agcaaggtag ggcttcgcaa agctagcgac attgacagag
     2821 tcccttttcc ccccttttcg tgtgacatca ataaccatta cattaaggtt aatatccttt
     2881 aaatctaagt ttactgcctc agatagacgc actcctgaag ctagtagcaa agcaataata
     2941 gctaaatctc tctctttatt tttgttgaaa aaagctaaag cacgtttgga aagtttgttt
     3001 tgatattcac aatctatata ctctaagaat tctatcgttt cattgcccaa aaataacttt
     3061 tgtttaatat tttctgcccg agaagctaaa gtttcctttt tctttttggt tgagactttt
     3121 ttcatgacat tgcgataaaa gtaaggctcc ccgtcagcat tctcaacctc ttcagtaagg
     3181 tatttgaaaa gactagataa ggcagataga gttctgttaa tggtggtttg agacacccca
     3241 ttttgccttg tgttagcatt taataaaggg cgttctcgta aatatagtat aaaagcttcc
     3301 atatcttttt tagtaaggtt ttctaaaact gaaagctcaa tttctgcaat gtgatgacaa
     3361 ttagcaacac cagaatccaa taaccattca agaaaacgtc tatattcttt taaatattcg
     3421 tagagtgttg tgaaactata aggaacagaa agctttgatt gataatattc tagaacatac
     3481 caaggcataa tttcttttag ttcat
//
LOCUS       DATROQ010000135         3489 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.136, whole genome shotgun sequence.
ACCESSION   DATROQ010000135 DATROQ010000000
VERSION     DATROQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.136"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..215)
                     /locus_tag="VBC94_001931"
     CDS             complement(<1..215)
                     /locus_tag="VBC94_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease
                     domain-containing protein"
                     /protein_id="HEO6980167.1"
                     /translation="MNKRILNSQFAVYKNWPSWLLLLFACFMVYLFFFVGLTLGGVGI
                     VLVSSVLYKFSSYTFFEFINLFNNIYG"
     gene            398..2581
                     /locus_tag="VBC94_001932"
     CDS             398..2581
                     /locus_tag="VBC94_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO6980168.1"
                     /translation="MKHKLKAFTLALLSIFFVFGGKVSAETVNIVSDTAYAPFEFKDS
                     DQTYKGIDVDIVNEVAKRAGWNVNMTYPGFDAAVNAVQSGQADALMAGTTVTEARKKV
                     FNFSDTYYDTSVILYTKNNNKVTNYKQLKGKVVGVKNGTAAQSFLEENKSKYGYKVKT
                     FDTSDLMNNSLDSGSIYAAMDDQPVVQFAINQGKAYAINMEGEAVGSFAFAVKKGSGH
                     DNLIKEFNTAFAQMKSDGTYNDIMDKWLGKDATKTSGKATGNANEKATPVKPSYKIVS
                     DSSFAPFEYQNGKGKYTGFDMELITKIAKQQGFKLDISNPGFDAALNAVQSGQADGVI
                     AGATITEARQKIFDFSDPYYTSSVILAVKKGSNVKSYQDLKGKTVGAKNGTASYTWLS
                     DHADKYNYHVKAFDEASTMYDSMNSGSIDALMDDEAVLAYAINQGRKFETPIKGEKSG
                     DIGFAVKKGANPELIKMFNNGLASLKKSGEYDKLVKKYLSTASTSSNDKAAKPVDEST
                     ILGLISNNYKQLLSGIGTTLSLTLISFAIAMVIGIIFGMMSVSPSNTLRTISMIFVDI
                     VRGIPLMIVAAFIFWGIPNLIESITGHQSPINDFVAATIALSLNGGAYIAEIVRGGIE
                     AVPSGQMEASRSLGISYGKTMQKVILPQAVRLMLPNFINQFVISLKDTTIVSAIGLVE
                     LFQTGKIIIARNYQSFRMYAILAIMYLVIITLLTRLAKRLEKRLK"
     gene            2581..3321
                     /locus_tag="VBC94_001933"
     CDS             2581..3321
                     /locus_tag="VBC94_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6980169.1"
                     /translation="MAELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLESITSGKVVVDGFELSNPKTDIDKARENIGMVFQHFNLFPHMSVLENI
                     TFAPIELGKESKEAAEKHGMELLEKVGLADKANAKPDSLSGGQKQRVAIARSLAMNPD
                     ILLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGRF
                     LEDGTPEQIFDTPQHPRLQDFLNKVLNV"
BASE COUNT         1210 a          609 c          616 g         1054 t
ORIGIN      
        1 tctccataga tattattaaa taaattaata aactcaaaaa aagtatagga agaaaactta
       61 tagagtacag aagacactag aacaattccc acaccaccta gagttagacc gacgaaaaaa
      121 aataggtata ccataaaaca agcaaataac aataatagcc atgacggcca gtttttatag
      181 acagcgaact gactatttaa aatcctttta ttcataataa tcctaaatcc tttttgtgtg
      241 atgacactag aacaattata tcataatact aactaaagat atatataaaa ttaacataac
      301 atacatgtta cgttttctaa catagaaatg acattttgtt gctctatctt atttattttg
      361 atataataaa acacatattt ataaaaagga gatttacatg aagcacaagt taaaagcttt
      421 tacgcttgct ttactctcaa tattctttgt gtttggtgga aaggtcagtg cagagactgt
      481 gaatattgtt tctgatacag catacgctcc attcgaattt aaagattctg atcaaactta
      541 taaaggaatc gatgttgaca tcgttaacga agtcgctaag cgtgctggct ggaatgttaa
      601 catgacgtat ccaggttttg atgccgcagt taacgctgtt caatctggac aggcagatgc
      661 gctaatggcc ggaactactg ttactgaagc acgtaaaaaa gtctttaatt tctcagatac
      721 ttattacgat acttccgtta ttctttatac taaaaataat aataaagtca caaactacaa
      781 acaactaaaa ggaaaagtag tcggtgtaaa aaatggaaca gctgctcaaa gcttcttaga
      841 agaaaataaa tctaaatacg gctataaagt taaaacattt gatacaagcg acctaatgaa
      901 taacagcctt gattctggtt ctatttacgc cgctatggac gatcaaccag ttgtgcaatt
      961 tgcgataaat caaggaaaag cttacgccat taacatggaa ggcgaagcag ttggtagctt
     1021 tgcatttgct gtcaaaaaag gtagtggaca cgataatcta attaaagaat ttaacacagc
     1081 ttttgcacaa atgaaatcag atggcactta taatgacatc atggataaat ggcttggaaa
     1141 agacgctaca aaaacaagcg gcaaagcaac aggtaatgcc aatgaaaaag caactcctgt
     1201 aaagccaagt tataaaattg tttctgattc ttcattcgca ccattcgaat atcaaaacgg
     1261 taaagggaaa tatactggtt ttgatatgga attaatcacg aaaattgcta aacagcaagg
     1321 ttttaaactt gatatctcaa atccaggttt tgatgccgct ttaaatgctg tccaatctgg
     1381 gcaagctgac ggtgttattg caggagccac aatcacagaa gcacgccaaa aaatctttga
     1441 tttttctgat ccttattaca catctagcgt tatcttagcg gttaaaaaag gaagcaatgt
     1501 caaatcatac caagatttaa aaggaaaaac agttggtgct aaaaatggta ctgcctcata
     1561 tacttggtta tcagaccacg cagataagta caactatcat gttaaagcat ttgatgaagc
     1621 atctacaatg tatgatagta tgaactcagg ttcaattgat gctctaatgg atgacgaagc
     1681 cgttcttgct tacgctatta atcaaggtcg taaatttgaa acacctatca aaggtgaaaa
     1741 atcaggcgat atcggatttg cagtgaaaaa aggggcaaat ccagaattaa ttaaaatgtt
     1801 taacaacggt cttgcttcac tcaaaaaatc gggtgagtac gataaacttg ttaaaaaata
     1861 cctttccaca gccagcactt cttcaaacga taaagctgct aaacctgtag atgaatcaac
     1921 tattttaggg ttaatttcta ataactacaa acaattgcta tctggtattg gaactacttt
     1981 aagtttaact cttatctcgt ttgcgattgc tatggttatt ggtattatct ttggtatgat
     2041 gagcgtatca ccaagtaata ctctccgcac aatttcaatg atttttgttg atattgtccg
     2101 tggtattcca ctcatgattg tggccgcttt tattttctgg ggtattccta atttaatcga
     2161 aagcatcaca ggtcaccaaa gtccaattaa tgacttcgtt gctgctacta tcgctctttc
     2221 tttaaatggt ggtgcgtaca ttgctgaaat tgtacgtggt ggtattgaag ctgttccttc
     2281 tggtcaaatg gaagcaagtc gcagcttagg tatttcttac ggcaaaacta tgcaaaaggt
     2341 tatcttacct caagcagtac gccttatgtt accaaacttt atcaaccaat ttgtcatctc
     2401 attaaaggat acaacaattg tatcagcaat cggacttgtg gaactcttcc aaactggtaa
     2461 aatcataatt gcacgtaact atcaatcatt ccgtatgtat gctatccttg caattatgta
     2521 tcttgtaatc attacccttc tcacccgtct agcaaaacgt ttagaaaaga ggcttaaata
     2581 atggcagaat taaaaattga tgtccaagat ttacacaagt cgtatgggca aaatgaagtt
     2641 ttaaaaggta ttgatgctaa attttatgaa ggtgatgttg tttgtattat tggaccttct
     2701 ggttcaggaa aatcaacttt ccttcgcaca ttgaacttac ttgaatcaat cacaagtgga
     2761 aaagttgttg tggacggctt tgagttatca aatccaaaaa cagacataga caaagcacgt
     2821 gaaaatatcg gaatggtatt tcaacacttc aatctttttc cacatatgtc agttcttgaa
     2881 aacatcactt ttgctcctat agaacttggt aaagaatcta aagaagcagc tgaaaagcat
     2941 gggatggaat tacttgaaaa agttggttta gccgataaag ctaatgccaa accagatagt
     3001 ttatctggag gacaaaaaca acgtgtagct atcgcaagaa gcttagcgat gaatccagat
     3061 atcttacttt ttgacgaacc aacttctgca cttgatccag aaatggttgg agatgttctc
     3121 aatgttatga aagatttagc tgaacaaggt atgactatgt taattgtgac acacgaaatg
     3181 ggatttgctc gtcaagttgc caatcgtgtt atctttacag atggtggtcg tttcctcgaa
     3241 gatgggacac ctgagcaaat ctttgataca cctcaacacc cacgtctcca agacttcctt
     3301 aataaagttt taaacgttta ataaaaaaag atgacttcgt gtcatctttt tttatatcct
     3361 gatcacaaac aaattataat aggataagtt ttcttctaaa attagcaaaa catcagctag
     3421 aatgtttgaa taatgttgca caagggcttt cagtatgata atatagcagt atgaaagact
     3481 atattaaca
//
LOCUS       DATROQ010000136         3444 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.137, whole genome shotgun sequence.
ACCESSION   DATROQ010000136 DATROQ010000000
VERSION     DATROQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.137"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..1529)
                     /locus_tag="VBC94_001934"
     CDS             complement(<1..1529)
                     /locus_tag="VBC94_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980170.1"
                     /translation="MVENLEKPIGVSYKNSPSMSKRTAIRMKKSSRFSILLYSVLSTL
                     LAIANPLLTYFANGLQTQNLYTGLMMTKGQIPYSDVFATGGFLYYVTIALSYLLGSSI
                     WLFIVQFIAYYVSGIYFYKLVYYVAQSEIVSIGMTLIFYIMNIVLGFGGMYPIQWALP
                     FMLISLWFLIKFCVDNIVDEAFIFYGILAAFSLFIDPQTLIFWLCSFVLLTATNIKQK
                     QSLRGFYQFLCVVFGMILIAYTVGYFMFNLQIISSYIDKAIFYPFTYFARTNHSFLLS
                     LAIQIVVLLGSGCLFGLWDFIQNRKKASYQIGLNFIACIFIIYAIMAIFSRDFNLYHF
                     LPALPFGLLLTSNKITILYQKVIDRRSHRRQYFSGKSLIVDLFVKKTYYLPLLLVSLL
                     IGLLVYNTYQNVTLSKERRDISHYLTTKIDRDGKIYVWDKVASIYSQTRLKSASQFVL
                     PHINTAQKNNEKILKDELLQHGAKYFILNKNEKLPNELKSDIKKHYQEVPLSNITHFV
                     L"
     gene            complement(1780..2097)
                     /locus_tag="VBC94_001935"
     CDS             complement(1780..2097)
                     /locus_tag="VBC94_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEO6980171.1"
                     /translation="MAHNHNHGHNHEHQHEVITLVDENGNETLFEILLTIDGREEFGK
                     NYVLLVPAGAEEDEQGEIEIQAYSFTENADGTEGDLQPIPEDSDAEWDMIEEVFNSFL
                     DEE"
     gene            complement(2123..2542)
                     /gene="ruvX"
                     /locus_tag="VBC94_001936"
     CDS             complement(2123..2542)
                     /gene="ruvX"
                     /locus_tag="VBC94_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEO6980172.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEESGNFGFDR
                     LAELVKEYKVDKFVVGLPKNMNNTSGPRVEASQVYGDKITELFNLPVEYQDERLTTVQ
                     AERMLVEQADISRGKRKKVIDKLAAQLILQNYLDRMF"
     gene            complement(2542..2808)
                     /locus_tag="VBC94_001937"
     CDS             complement(2542..2808)
                     /locus_tag="VBC94_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEO6980173.1"
                     /translation="MGFTDETVRFRLDDSNKVEISETLTAVYRSLEEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDL"
     gene            complement(3010..3408)
                     /gene="spx"
                     /locus_tag="VBC94_001938"
     CDS             complement(3010..3408)
                     /gene="spx"
                     /locus_tag="VBC94_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEO6980174.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHQLPYKEQNLGKESLTRDEILE
                     ILTKTESGIESIVSSKNRYAKALNCNIEELSVNEVIDLIQENPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNVENAEARLRAAL"
BASE COUNT         1157 a          637 c          518 g         1132 t
ORIGIN      
        1 tatagaacaa aatgagtgat atttgataga ggaacttctt ggtagtgctt cttaatatca
       61 gatttcagtt catttggtaa cttctcattt ttatttaaga taaagtattt agccccatgc
      121 tgaagcagct cgtccttcaa aattttctca ttattttttt gcgctgtatt aatatgtggc
      181 aaaacaaatt gcgaagcaga ttttaaccga gtctggctat aaatgctagc aactttgtcc
      241 caaacataaa tcttaccatc tctatcaatt tttgtagtca aataatgtga aatgtcacgc
      301 ctttctttac taagagtaac attttgataa gtattataaa caagtaaccc tatcaataag
      361 gaaactagta agaggggcaa ataatatgtt ttctttacaa aaaggtctac gataagagat
      421 ttaccagaga aatactgacg tctatgtgaa cgcctgtcaa tcactttttg gtaaagaata
      481 gtaattttat tactcgttag caaaagacca aatggtaaag caggtaaaaa gtggtaaagg
      541 ttaaaatctc ttgaaaaaat ggccattata gcgtaaataa taaaaataca ggcaataaag
      601 ttcaagccga tttgataaga tgctttttta cgattttgaa taaaatccca tagtccaaag
      661 aggcaacctg accctagaag gacaacaatt tgaattgcta aactaagaag gaatgagtgg
      721 tttgtcctag cgaaatacgt aaaggggtaa aaaatagctt tgtcaatata agaagagata
      781 atctgtagat taaacataaa gtaaccaaca gtataagcaa ttaaaatcat accaaaaaca
      841 acgcataaaa actgatagaa ccctcgtagt gactgctttt gcttaatatt ggttgctgtt
      901 aagagaacaa aagagcaaag ccaaaaaatc agtgtttggg gatctataaa tagtgagaat
      961 gctgctaaaa taccataaaa tataaatgct tcatcaacga tattatcgac acaaaattta
     1021 attaaaaacc atagcgaaat gagcataaaa ggtaatgccc actgtattgg gtacatacca
     1081 ccgaatccta agacaatatt cattatatag aaaatcaacg tcatgcctat cgagacaatt
     1141 tcactttgtg ccacataata aactaattta taaaaataaa ttccagatac atagtaagca
     1201 ataaactgta caataaataa ccagatacta gaccctaaaa ggtaacttag agcaatcgtt
     1261 acatagtata aaaatcctcc tgtagcaaaa acatcactat aagggatctg tcctttggtc
     1321 atcatcaaac ctgtatagag attctgagtc tgcaaaccat ttgcaaaata agtcaataaa
     1381 ggatttgcaa ttgctaagag agttgacaaa acactatata gtaaaattga aaatcgacta
     1441 gatttcttca ttctgatagc cgttcgttta ctcattgatg ggctattttt atagctcact
     1501 ccaattggtt tttctaggtt ttctaccact atttttctcc tcatatttac atactaccag
     1561 tatatcagaa aaccttattt agtcaattta gccaacactt gaccgtaacg aataatatcc
     1621 ctaaagtagt ggtatcttct agacatatat tgtttgagaa attcttgttc ttctaatgtt
     1681 agctgagtat ctgacattaa catcacctcc ttttactaca tcatcactat tattattcga
     1741 aaaaaaaaac gactgaacaa ttgctcaatc gcttcaatat tattcttcat ctagaaagct
     1801 gttgaaaact tcctcaatca tgtcccattc tgcatctgaa tcttctggta taggctgaag
     1861 gtctccttca gttccatcag cattttctgt aaatgagtaa gcttgaatct caatttcacc
     1921 ttgttcgtct tcttctgcac ctgcaggaac gaggagaaca tagtttttgc caaactcttc
     1981 acgtccatca atagttagta ggatttcaaa aagtgtttcg ttcccattct catctacaag
     2041 agtgattact tcatgttgat gttcatggtt atgaccgtgg ttatgattat gtgccattgt
     2101 ttctcctttt tatgccgaat gcttaaaaca tgcggtctaa ataattttgt aagattagtt
     2161 gagctgctaa tttatcgata actttcttac gcttaccacg actaatatca gcttgctcaa
     2221 ctagcatacg ctctgcttga acagtcgtta gacgttcatc ttgatattca actggaagat
     2281 tgaataattc agtgatttta tcgccatata cctgactagc ctctacacga ggaccacttg
     2341 tattattcat atttttaggt agaccaacta caaatttatc aactttgtac tcttttacta
     2401 actctgctaa gcggtcaaaa ccaaaatttc cagattcttc atcgatctta atgatttcaa
     2461 gaccttgtgc agtgaaaccg agtggatcac taattgcaac gccaacagtt ttcgagccga
     2521 cgtcaagtcc cattattctc attacaaatc tattccattt ccttgtaaat aataacgaac
     2581 gagctcttct acaatctcat cacgttcata tttacgaatt tgattgcgag catcattgta
     2641 acgaggtaca taagcagggt ccccactcaa tacgtaacca acaatttgat tgatgggatt
     2701 gtaacctttt tcttctaatg aacgataaac agctgttaat gtttcactaa tttctacttt
     2761 gttactatca tctaatctaa aacgaacagt ttcatctgta aatcccatac ctacaccttc
     2821 tttcctttaa ttctttcact tattataact taaataaagt atttgtacaa gaaattgata
     2881 aacactatat tagaaaatca aaaagggagc acaagaaacc aagaggtgat tctttgattt
     2941 ctttactccc taccttatag gtgattttta caggttatca tgtgactctc acttatgctt
     3001 atctaaagct caaagtgctg cacgaagacg agcttctgca ttttcaacgt tacgaataga
     3061 gcgaggtaga aaagcacgaa tatcatcttc cttataacct acttgaagac gtttgtcatc
     3121 aattagaatt ggactcttta aaatacgtgg attttcttga ataagatcaa taacttcatt
     3181 tacactcaac tcctcaatat tacaattgag cgccttagca taacgatttt tagaagaaac
     3241 gatactttca ataccacttt cagtttttgt taatatttct aaaatttcat cacgtgttag
     3301 tgattcttta cctaggtttt gttctttata tggaagttga tgagcattta gccaagtttt
     3361 tgctttttta caacttgtgc aacttgaaat agtataaatc ttaatcatgt gccttctcac
     3421 tttctatccc atgatagtta tagt
//
LOCUS       DATROQ010000137         3230 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.138, whole genome shotgun sequence.
ACCESSION   DATROQ010000137 DATROQ010000000
VERSION     DATROQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.138"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            187..1209
                     /locus_tag="VBC94_001939"
     CDS             187..1209
                     /locus_tag="VBC94_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6980175.1"
                     /translation="MNIIIGTSLLILVLAIFTLFNYKAPYGTKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVTIAIGVTPGYAVLIGLSVSGTG
                     IIPGFLAGYLVGFLVKWMERNIPGGLDLISIIIIGAPLTRLVAKLLTPLINSTLLTIG
                     DILTSGAHSNPILMGVILGGTIVVVATAPLSSMALTAMLGLTGMPMAIGALSVFGSSF
                     MNGVLFHKLKLGSRKDNIAFAVEPLTQADVTSANPIPIYVTNFVGGAACGILIALMKL
                     VNDTPGTATPIAGFAVMFAYNPMIKVLITALGCIILSLLAGYFGGIVFKDYKLVTKEE
                     LQARDN"
     gene            1580..2872
                     /locus_tag="VBC94_001940"
     CDS             1580..2872
                     /locus_tag="VBC94_001940"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEO6980176.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDNKKFKLHLIPSGIFFKEKISVIGNGVVVNPKSLVKELAYLHGEGVTTDNLRISDRA
                     HVILPYHIKLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDREVFAERL
                     KINLAEKNRLFEKMYDSTPLEFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGDRIREIGKEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWSEDITACRSLDDLPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWSNI"
     gene            complement(2895..3188)
                     /locus_tag="VBC94_001941"
     CDS             complement(2895..3188)
                     /locus_tag="VBC94_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980177.1"
                     /translation="MILINLIALPICFLAELSAGLLLKLEMHKQHGYYPLERLLMTGS
                     ILTVVSLVLLHTLIHNINSLLFLSIIVVLLISGKSLSLISIYLLKRKSKHQKV"
BASE COUNT          975 a          523 c          644 g         1088 t
ORIGIN      
        1 ttagcaacta aggagtaatc catgagataa gcctaataat tatgtcggac ttctttgttg
       61 caccttatcc agttcgtagt acaattaagt ttcagtatta cctaaaggcg cctttattga
      121 aattgaagca gtagtacaca gacactgaga ataaaaaact taactatttt tagcgggagt
      181 atatttatga atatcattat tgggaccagt cttttaatac ttgtattagc gattttcact
      241 ttatttaatt ataaagcacc ctacggaaca aaagcaatgg gagcattggc atctgcggct
      301 tgtgcatcct tccttgtaga agcttttcaa gattctttct ttggtaaagt tttgggattt
      361 caatttttaa gtgaagtggg tggtgctaat ggttcacttt ctggtgtagc tgcagctata
      421 ttggttacta ttgctattgg tgtaacccca ggatacgcag ttttaattgg tttatctgtt
      481 tctggaacag gtattattcc tggtttcctt gcaggatatt tagtgggatt tcttgttaag
      541 tggatggaaa gaaatattcc tggtggttta gatcttattt caattattat tattggtgca
      601 ccattaacac gtcttgtggc caaactgttg acgccgctta ttaatagcac tttgctcacc
      661 attggagata tcttaacatc aggtgctcat agcaacccta ttttaatggg ggttatactt
      721 ggcgggacaa ttgtagtagt ggcgacagca ccactttctt ctatggcatt gacagctatg
      781 ctaggattaa ccggaatgcc tatggctata ggagccttgt ctgtctttgg ttcgtcattt
      841 atgaatggtg tacttttcca taaattaaaa cttggaagtc gtaaagataa tatagctttt
      901 gctgttgagc ctctaactca agctgacgtg acttcagcta accctattcc aatctatgtc
      961 actaattttg ttggtggtgc agcttgtggt attttaattg ccttgatgaa attagttaat
     1021 gatactcctg gaacagcgac accaattgca ggatttgctg tcatgtttgc ctataaccca
     1081 atgataaaag tactaataac cgctctaggt tgtattatcc tatctttact agcaggctat
     1141 tttggaggca ttgtttttaa agattataaa ttagttacaa aagaagagct acaggctaga
     1201 gataactagt tacttcatga aaagctaata gtaaatatca ttaatataga tatgatatct
     1261 gacttgtgat aactgttatt gataatttaa aattcaacga ctctaataaa aagattgttg
     1321 aattttttta ttgttaagaa tgctggtatc agtaaatttc aagatttctt tatcaactat
     1381 aatgttagtt ttttaccgtt tttttacact tctaataatt atttttttct ttttttccga
     1441 taatatttga ggaatttggc aaaaaaaaac attttttgct ataataaggt gtcagacaaa
     1501 ctcgcaggtg aaattcctgc catgtttttc cagaaaggat tggggagtcc caaaaaggct
     1561 cactcagtgt taaataaata tgacttcagt tgttgtagta ggtacccaat ggggtgacga
     1621 aggtaaaggt aaaattacag attttttatc tgctgacgca gaagtaattg cacgttatca
     1681 aggtggtgat aacgctgggc atactatcgt tattgataac aaaaaattta aacttcattt
     1741 aattccttca ggaattttct tcaaagaaaa aatttctgtc attggtaatg gtgttgtcgt
     1801 taatccgaaa tcattagtta aagagttagc ttatcttcac ggagaaggtg taacaacgga
     1861 taaccttcgt atttcagatc gtgcgcatgt tatcttgcca taccacatca agcttgacca
     1921 actacaagaa gatgctaaag gtgataataa aattggaaca accatcaaag gaattgggcc
     1981 agcttacatg gacaaggctg cgcgtgtcgg tatccgtatt gctgaccttc ttgatagaga
     2041 agtttttgct gagcgtttga aaattaattt agctgagaaa aatcgtttat ttgaaaaaat
     2101 gtatgacagt acacctcttg aatttgatga tattttcgaa gaatattatg aatatggtca
     2161 acaaattaag caatatgtga ctgatacatc cgttatctta aatgatgctc ttgatgccgg
     2221 taaacgtgtt ctttttgaag gagcacaagg agttatgctc gacattgatc aaggaacata
     2281 cccatttgta acatcttcca atccagtagc aggtggtgtc acaattggtt cgggagttgg
     2341 accaagtaaa attaataaag tggtaggtgt atgtaaagct tacactagcc gtgttggcga
     2401 tggaccattc ccaacagaac tttttgatga ggttggtgac cgtattcgtg agattggtaa
     2461 agagtatggt acaacgactg gtcgtcctcg tcgcgttgga tggtttgatt ctgttgttat
     2521 gcgtcacagc cgtcgagtat caggtattac taacctctct ctgaattcaa ttgatgttct
     2581 ttcagggctt gatacggtga aaatttgtgt ggcttatgac cttgatggga aacgtattga
     2641 ctattaccca gcaagccttg aacagctaaa acgttgtaaa ccaatctatg aagaattacc
     2701 gggctggtct gaagatatta cagcttgccg tagcttagat gatcttccag aaaatgcacg
     2761 taattacgtt cgccgtgttg gcgaattggt tggtgttcgt atttctactt tctcagtagg
     2821 gcctggtcgt gaacaaacaa acattcttga gtctgtttgg tcaaatattt aatattaaaa
     2881 atctgactgt acagtcagac tttttgatgt ttactttttc tttttaatag atagatacta
     2941 ataagactta aggacttccc agaaatcaat aaaactacta taattgataa aaaaagaagt
     3001 gaattgatat tgtgaatcaa agtatgtaat aatactagcg atacaactgt tagaatacta
     3061 ccagtcatca ataagcgttc cagaggatag tagccatgtt gcttatgcat ttctaatttt
     3121 aatagtagac cagctgacaa ttctgctagg aagcaaatag gtagagctat caaattaata
     3181 agaatcatag accaaccaaa tccttgacca taacctatat atgatagact
//
LOCUS       DATROQ010000138         3187 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.139, whole genome shotgun sequence.
ACCESSION   DATROQ010000138 DATROQ010000000
VERSION     DATROQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.139"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            85..861
                     /locus_tag="VBC94_001942"
     CDS             85..861
                     /locus_tag="VBC94_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6980178.1"
                     /translation="MKLTTLGCWGGYPYKDGGTTSFLLTGENNFHLLIDAGSRSINEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLYHYRLLYPKDKWEPKVLPVYGHNEDDYEFS
                     KLTLPNVAQGIAYHTNGVEQVGPFNITFIKTVHPVVCYAMRITERETGQVLVFTGDSG
                     YFADLVPFTKGADLFLADVYLYEGNENHIAHLTSKEAGEIAYNAKVKKLVLTHVPQFP
                     GEGINPENHLEELRLQSEKYAGGISVDLAQPHKSWDLGKL"
     gene            861..1367
                     /locus_tag="VBC94_001943"
     CDS             861..1367
                     /locus_tag="VBC94_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEO6980179.1"
                     /translation="MQKFTQEEKEWFMTEALKEAKSSLEKEEIPIGCVIVKDGHIIGR
                     GHNAREEFNKAILHAEIMAINNANEKVGNWRLLDTTLFVTVEPCVMCSGAIGLARIPH
                     VIYGAKNTKFGAAGSLYDILSDSRLNHRVEVETGILEDQCAKIMQDFFRKGRQRKKEA
                     KLSEKTKI"
     gene            1402..1487
                     /gene="ffs"
                     /locus_tag="VBC94_001944"
     ncRNA           1402..1487
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VBC94_001944"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1639..2988
                     /locus_tag="VBC94_001945"
     CDS             1639..2988
                     /locus_tag="VBC94_001945"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEO6980180.1"
                     /translation="MTHITFDYSKVLGQFVGEHELDYLQPQVSAADAFLRQGTGPGSD
                     FLGWMDLPENYDKEEFSRIQKAAEKIKSDSEVLVVIGIGGSYLGAKAAIDFLNNHFAN
                     LQTAEERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVVRVEK
                     PRKNVTIPELTEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPTQDAY
                     TLGYTIYFFELAIGLSGYLNSVNPFDQPGVEAYKRNMFALLGKPGFEELSAELNARL"
BASE COUNT         1077 a          503 c          645 g          962 t
ORIGIN      
        1 gtcttttttg tgcataaaat ctaaaacagt tattcttaac aaaatttggt ataatagcat
       61 taaatcagta aggaggggat tatcatgaaa ttaacaacgt taggttgttg gggtggttat
      121 ccttataaag atggaggaac cacaagtttt ctattaactg gagaaaataa ttttcactta
      181 ttaatagatg cgggaagtcg ttcaatcaat gagttagaaa aagaaataag cccgttggat
      241 ttggatgcag ttatcatcag tcattatcat ccagatcatg tagcagattt gggtgtgttg
      301 tatcattatc gtttgctata ccctaaagat aaatgggaac ccaaagtatt gccagtttat
      361 ggacataatg aggatgatta tgagttttct aaattgacat tacctaacgt tgctcaagga
      421 attgcttatc ataccaatgg agttgagcaa gttggtccct tcaatattac gtttatcaaa
      481 acagtccacc cagtcgtctg ctatgccatg aggattacag aaagagaaac tggtcaagta
      541 ttagtcttta caggagattc tgggtatttt gctgaccttg taccttttac aaaaggcgca
      601 gatttatttt tagcagacgt ttatctttac gagggaaatg aaaatcatat tgcacatttg
      661 actagtaaag aagcaggtga gattgcttat aacgctaaag ttaagaaatt agttttaaca
      721 catgttccgc agtttccggg agaaggaata aaccccgaaa atcatttgga agaacttcga
      781 ctacagtctg aaaagtatgc aggtggcatc tctgttgatt tagctcagcc acataaatct
      841 tgggacttag gaaaactata atgcagaaat ttacccaaga agaaaaagaa tggtttatga
      901 cagaagctct aaaggaggca aaatcctcct tagagaaaga agaaattcct ataggttgtg
      961 tcattgtaaa agatggtcat attattggaa gaggacataa tgctcgtgaa gagtttaata
     1021 aagctatttt acatgctgaa atcatggcta ttaacaatgc aaatgagaag gtagggaatt
     1081 ggcgcttatt agatactact ttatttgtta ctgtagaacc ttgtgtaatg tgtagtggag
     1141 ctattggctt agctagaatt ccccacgtta tatatggcgc taagaatacg aaatttggag
     1201 cagcgggaag tctctatgat attttaagtg atagccgact taatcataga gttgaagttg
     1261 aaaccggcat tttagaagat caatgcgcta aaataatgca ggatttcttt cgaaaaggac
     1321 gacaacgcaa gaaagaagct aaactaagtg aaaaaactaa gatttgatag cttgcaataa
     1381 aatattagtt ttgttataat atctttggag caacatttac tcgtgaagtg ggtcagggga
     1441 ggaatccagc agccctaagc gatgctaaat gtgtgctctt tttattttca aaagtcaaag
     1501 tgtttttgga aataattatg aaagtttcat aaaaattggt agcgtttcaa aaaatatgaa
     1561 aatacagtta aaaaaccttg aaaaaaaact cacaaaagag taagatgata gtgaaacata
     1621 attgttagga gaaataaaat gacacatatt acatttgact attcaaaagt cttgggtcaa
     1681 tttgtaggcg aacacgagtt agactaccta caaccacaag taagtgcagc agatgctttc
     1741 cttcgtcaag ggactggtcc tggatcagat tttctcggat ggatggacct cccagaaaac
     1801 tatgacaaag aagaattttc tcgcattcaa aaagccgctg aaaagattaa atcagatagc
     1861 gaagtactcg tggttattgg tattggtggt tcgtaccttg gtgcaaaagc agcaattgac
     1921 tttttgaata atcattttgc taatttgcaa accgcagaag aacgtaaagc gcctcagatt
     1981 ctttatgctg gaaattctat ttcatctact taccttgccg atttagttga atacgtccaa
     2041 gataaagaat tctcagtaaa tgtcatttca aaatcaggta caacaactga accagcgatt
     2101 gctttccgtg tatttaaaga acttctagtt aaaaagtacg gtcaagaaga agctaataaa
     2161 cgtatctacg ctacaactga taaagttaaa ggtgccgtaa aagttgaagc agatgctaac
     2221 aattgggaaa catttgtagt tcctgataat gttggtggtc gtttctcagt actgacagct
     2281 gtaggtcttc ttccaattgc tgcttcagga gcagatatta ctgctcttat ggaaggcgca
     2341 aatgctgctc gtaaagattt atcttcagat aaaatctctg aaaacattgc ttatcagtac
     2401 gctgcagttc gtaatgttct ttatcgcaaa ggttatatca cagagatttt agctaactac
     2461 gaaccatcat tacaatactt tggtgaatgg tggaaacaat tagcaggtga atcagaaggt
     2521 aaagatcaaa aaggtatcta tcctacatca gcaaacttct caactgatct tcactcactt
     2581 ggtcaattta ttcaagaagg ttaccgtaac ctctttgaga cagttgttcg cgttgagaaa
     2641 cctcgtaaaa atgtgactat tcctgaatta accgaagatt tagatggtct tggttatctt
     2701 caaggaaaag atgtagattt tgttaataaa aaagcaacag atggtgtgct tcttgctcat
     2761 acagatggtg gggttccaaa tatgtttgta acgcttccta cacaagacgc ttacactctt
     2821 ggttacacta tttacttctt tgagttagca attggccttt caggttatct taactcagta
     2881 aatccatttg atcaaccggg ggtagaagca tataaacgta atatgtttgc tcttctaggt
     2941 aaacctggat tcgaagagct tagcgctgaa ttgaatgcac gtctttaaaa atatctaact
     3001 aaaaacccac tagcactggt agcgatagtg ggttggaaaa agggttgaga aaagttatta
     3061 acttttctca ggagatttat gaaaaaggaa aataatttag gataatattt tcctaacggc
     3121 taggataaac cgcttatttt aggatgatgc tagtatacag tagaaacctt aaataaacct
     3181 taaaaat
//
LOCUS       DATROQ010000139         3134 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.140, whole genome shotgun sequence.
ACCESSION   DATROQ010000139 DATROQ010000000
VERSION     DATROQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.140"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            24..482
                     /locus_tag="VBC94_001946"
     CDS             24..482
                     /locus_tag="VBC94_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980181.1"
                     /translation="MRKYIKWLIPISIFGMILGGCQMNSEPKSQSNEVKNSKQSEVKK
                     DKKMTKKEQLAYLKEHEQEIIDYVKLHNNQIESVQFDWSSVKVEQSGNGTPQGGDYNL
                     SLRGKFNHLQNSKLIVDFYLAHKNDIPNIKSMGMLNKPYIHKNGIWHIYE"
     gene            546..2180
                     /locus_tag="VBC94_001947"
     CDS             546..2180
                     /locus_tag="VBC94_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6980182.1"
                     /translation="MSNWNLDNFDNLHSSLAESAYNSRPNSFPELFETDSVTEVKFSQ
                     PSEDNKGQITQGGTNLPNDGIVYLQPDKSLKSIDENVKVLIPDVNGGYHTEHYVTHSY
                     QKGVLTDDKAGFNAYYLSDTEKIDSTTKHTYLAIRGSDGIGLDTLNDWVSNNAMFAVS
                     NKYIPQAKLANKAMKEKIAELKGKAPGAVMDITGHSLGTIVSSQAVVNLSYAELENVG
                     QVVLFDGPDVSRSLEKMEGISAKKIQEAGKHVTYYVNPFDIVSMLNREKPWKDQFGKV
                     NVIVPSKYTGTFDEQSSHDFGAFQIDSSGNPLVASESYHPELLTSGQDLARLEKKFIK
                     NLLGEHYDSDKLGPVLKGALTGDLTRLIATLKACGISVTVNEAKKLYDNFQKDYKAII
                     SKAKKDGLEWDRKNIASFHSRIKSASGSQRILLRTQMLYMATQLAEADVSEKVSATKK
                     HISEAKDTIVEISTSTISSAEIMAMHLGQSEFDALVSDIKMSTVWNDSVETSDYEALD
                     HYKTKMTTFTTNLVTVAQNLTAQDEQLAGDIVTNLS"
     gene            2199..2471
                     /locus_tag="VBC94_001948"
     CDS             2199..2471
                     /locus_tag="VBC94_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980183.1"
                     /translation="MSKELKLPLNKMLKEQERIAIKAIVATAKSTITDTGTNLKATIE
                     TDAQDLKNYASTYNANITDGLEGQSAQAASDFLTQLPKPELDNPVN"
     gene            2481..2831
                     /locus_tag="VBC94_001949"
     CDS             2481..2831
                     /locus_tag="VBC94_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980184.1"
                     /translation="MVDTKKLQELDQEYDQNLRNIYRNREQLEDDFHLFMARTDSLKE
                     SVYQATLGQGWELPQEAHAHLYNMDDNKDTFISEFNEYMEKLEEKEIELRRVYNDRVD
                     ELYQKAKQNEAKKG"
BASE COUNT         1186 a          524 c          575 g          849 t
ORIGIN      
        1 atattctaag aaaagagaac atgatgagaa aatatataaa atggttaatt cccatatcaa
       61 tttttgggat gatacttgga ggctgtcaaa tgaatagtga acctaaaagt cagtcaaacg
      121 aagtaaaaaa tagcaagcaa tcagaagtga agaaagataa aaaaatgaca aaaaaagaac
      181 aattagccta tctcaaagag catgagcaag aaatcataga ttatgtaaaa ttacataaca
      241 accaaattga gtccgttcaa ttcgattggt caagtgtaaa agtagaacaa agcgggaatg
      301 gaactccaca agggggtgat tataatcttt cactgagagg aaagtttaat catctacaaa
      361 attcaaaatt aatagttgat ttttatttag ctcataaaaa tgatatccca aatatcaaat
      421 caatgggaat gctaaataag ccatatatac ataaaaatgg tatttggcac atttatgaat
      481 agcttgaatc atcctatata aagtaataga atttttacca caagcaaggt agaggagaag
      541 aataattgag taattggaac ttagataatt ttgataactt acatagctct ttagcagaaa
      601 gtgcatataa tagtcgacct aattcatttc ctgaattatt tgagactgac tcagtaacag
      661 aagtaaaatt ttctcaaccc tcagaagata acaaaggtca aatcacacag ggtggcacta
      721 acctccccaa tgatggtatt gtctacctgc aaccagataa gtccttaaaa tctattgatg
      781 aaaatgtaaa ggtcctcatt ccagatgtca atggaggcta tcacactgag cattatgtta
      841 ctcattctta tcaaaaaggt gtgcttactg acgataaggc aggttttaat gcttactacc
      901 tgtcagatac tgaaaaaata gatagcacta caaagcacac ttatctagcc attcgtggta
      961 gtgatgggat tggtctagat acactaaacg attgggttag taacaatgca atgtttgctg
     1021 tctctaacaa gtatattcca caagcgaaat tggctaataa ggcaatgaaa gaaaaaattg
     1081 ccgagctaaa aggaaaagca cctggagctg ttatggatat tactggtcac tcactcggta
     1141 caattgtatc atctcaagca gtagttaatc ttagctatgc agaattggag aatgttggtc
     1201 aagtagtcct tttcgatggc cctgatgtat caagaagctt ggaaaaaatg gaaggcattt
     1261 ctgctaaaaa aatccaagaa gctgggaagc atgttaccta ctatgtcaat ccctttgata
     1321 tagttagtat gcttaatcgt gaaaagccat ggaaagacca attcgggaaa gtaaatgtca
     1381 ttgttcctag taaatatacg ggtacttttg atgagcaatc ttctcacgat tttggtgcct
     1441 tccaaattga tagtagcgga aatcctttag ttgcttctga aagctatcat ccagaattat
     1501 taacatctgg tcaagactta gctagactcg aaaaaaaatt cataaagaat cttttaggag
     1561 agcattatga ttcggataag ctaggaccag tactcaaagg cgctctgact ggggacctga
     1621 ctcgactaat tgcaacatta aaagcttgtg gtatcagtgt cactgtaaac gaagctaaaa
     1681 aactgtacga taacttccaa aaagactaca aagccataat ttccaaagct aaaaaagatg
     1741 gcctcgagtg ggatcgcaaa aacatcgcaa gcttccatag taggatcaaa tcagcatcag
     1801 gcagtcaacg gattttactt agaactcaaa tgctctatat ggcaacgcaa ttagcagaag
     1861 cagatgtttc agaaaaagtc tctgcaacaa aaaaacatat tagtgaagct aaggatacga
     1921 ttgtagaaat atcgacatcc actatttcat cagctgaaat aatggctatg caccttggcc
     1981 aatcagaatt tgatgctctg gtttcagata taaaaatgtc aacagtttgg aatgatagcg
     2041 tagagacttc tgattatgaa gcacttgatc actataaaac aaaaatgacc acatttacga
     2101 ccaatttagt aacagttgca caaaatttaa cagctcaaga tgagcaacta gctggtgata
     2161 ttgtcacaaa cttatcctaa aatagagaga ttaaatgaat gtccaaagaa ttgaaattgc
     2221 ccttgaataa aatgttgaaa gaacaagaaa gaatagctat taaggcaata gttgctacag
     2281 caaaaagcac tattacggat actggaacaa atttaaaagc aaccatagag acagatgccc
     2341 aagacctaaa aaactatgca tccacctata acgccaatat cacggatggt cttgaaggcc
     2401 agtcagctca agcagcatct gatttcttga cacaacttcc aaaaccagag ttagataatc
     2461 cagtcaacta ggaggctagt atggtagata caaaaaaact gcaagaactc gatcaagagt
     2521 atgatcaaaa tctcagaaac atttatcgta acagagagca actagaggat gactttcact
     2581 tgtttatggc aagaacagat agcctaaaag agtctgtcta tcaagcaact ttaggtcaag
     2641 gttgggaatt accccaagaa gctcatgcac acctatataa tatggatgat aataaggata
     2701 cattcatatc agaattcaat gaatatatgg aaaagcttga agaaaaggaa atagagcttc
     2761 gaagagttta taatgataga gtagatgaat tgtatcagaa ggctaagcaa aacgaagcaa
     2821 aaaaaggata aaaatttttt caagtaatat atataaatat gaaaaataaa gaatcttgca
     2881 gtaaaatgag tatttatttt tcacgacgaa cagtcactac aaaatcacta aaatctcaaa
     2941 attacttggt ttttaagtta atgtctaata ctcaaacctt gttaaatcaa tgtttgttat
     3001 ctttaaaaag tgctcagtag cactcaaaat ccagtgtcct cacggacgtg tcggttcgac
     3061 cccagccgcc ggtatacttt aaaagataag gtttctagac cttgtctttt ttgttttgtt
     3121 agataattta ttgt
//
LOCUS       DATROQ010000140         3130 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.141, whole genome shotgun sequence.
ACCESSION   DATROQ010000140 DATROQ010000000
VERSION     DATROQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.141"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            9..581
                     /locus_tag="VBC94_001950"
     CDS             9..581
                     /locus_tag="VBC94_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO6980185.1"
                     /translation="MRKRSILPILAVLFVFMSIFSVYSIFHGDKAKEKASVIKQASQT
                     SQTSKKEVLQKKTYPNLNKYSNLEIHVSSTRQTMTITSSDKVIFKTIVSTGAKESPTP
                     KGTFVIEPERGDFFYNASSKEGAYYWVSFKEHGIYLFHSVPTDQQGNEIPEEAKQLGK
                     AASHGCVRMSRADAKWFYENIPQGTTVTIK"
     gene            857..1708
                     /gene="ylqF"
                     /locus_tag="VBC94_001951"
     CDS             857..1708
                     /gene="ylqF"
                     /locus_tag="VBC94_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEO6980186.1"
                     /translation="MATIQWFPGHMSKARRQVQENIKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADPIRTKEWRDFYESQGLKTLAINSKEQSTVKKVTDIAK
                     ILMSDKIAKLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTYY
                     PDRLKERFKSINLEDESPEIIMTLTQKLGYRDDYDRFYNLFVKEVRDGKLGRYTLDIV
                     GEHDGNN"
     gene            1695..2456
                     /locus_tag="VBC94_001952"
     CDS             1695..2456
                     /locus_tag="VBC94_001952"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEO6980187.1"
                     /translation="MATIKEIKAILETIVDLKDKRWQEYQTDSRAGVQKAILQRKKNI
                     QSHLDEEARLEQMLVYEKKLYIEHINLIAGIDEVGRGPLAGPVVAAAVILPPNCKIKH
                     LNDSKKIPKKKHQEIYQNILDQALAVGIGIQDSQCIDDINIYEATKHAMIDAVSHLSV
                     VPEHLLIDAMVLDLPIPQTKIIKGDANSLSIAAASIVAKVTRDKIMSDYDSKYPGFAF
                     SKNAGYGTKEHLEGLQKYGITPIHRKSFEPIKSML"
     gene            2477..3025
                     /locus_tag="VBC94_001953"
     CDS             2477..3025
                     /locus_tag="VBC94_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEO6980188.1"
                     /translation="MTEKEKMLAGQYYRPSAPELRKDREVALKNMQAFNNEDDSSKRN
                     VILQKWFGATGKSIHMEQRFVCDYGCNIYVGENFYANFNQTFLDVCEIRIGDNCMFGP
                     NCQLLTPLHPLDPIERNSGLEYGAPIQIGNNVWLGGGVTILPGVVLGDNVVVGAGSVV
                     TKSFENNVVIAGNPAKIIKKLA"
BASE COUNT         1147 a          512 c          565 g          906 t
ORIGIN      
        1 gatagtacat gagaaaacgt tcaattttgc caatattagc tgtcttattt gttttcatga
       61 gtatctttag cgtatactct atttttcatg gtgataaagc taaagaaaaa gcaagtgtca
      121 taaaacaagc atctcaaaca agtcaaactt caaaaaaaga ggtcttacaa aagaagactt
      181 atccaaatct aaataaatat tcaaatctag aaattcatgt ttcatctacg aggcaaacaa
      241 tgacaataac ctcgagtgac aaggtcatct ttaaaacgat tgtttcaaca ggagcaaaag
      301 agagccctac cccaaaagga acttttgtca tagaaccgga acgaggagat tttttctata
      361 acgcctcatc aaaagaagga gcctattatt gggtctcctt taaagagcat ggtatttatc
      421 tctttcatag cgttccgact gatcaacaag gaaatgagat cccagaagaa gcaaagcaat
      481 taggaaaagc cgcctcacat ggctgtgtgc gtatgtcaag ggcggatgca aaatggttct
      541 atgaaaatat tcctcaagga acgactgtta ccatcaaata gtcttaatgt ggtaaaaatc
      601 atgttattaa aagcttggag taaacttcca gagctttttc gtatatccct taaactcaat
      661 tagtaaacga aacctatcag cggaataaat gtataaagta tttcgatatc attaaaaaaa
      721 tgatagaata atagtgttaa tcgaatattt tatcgacctt tatatgcggt tgaagaagaa
      781 taaaactaaa agattagacc tctaacaaat agatgtagac ttttaaggta gtctatacat
      841 aaggagaatt tatttcatgg ctactattca atggttccca ggacacatgt ccaaagctcg
      901 ccgtcaagtt caagaaaata ttaagcatgt tgattttgtt acaattttag tggatgcaag
      961 gttacctctt tctagtcaaa atccaatgct aaccaaaatc gtcggagaca aacccaaact
     1021 aatgatcctc aataaagctg atctagcaga tcccattcga actaaagaat ggagagattt
     1081 ttatgaatct caaggtttaa agacattagc tattaattct aaagaacaat caacagttaa
     1141 aaaagtaacc gacatcgcta aaatattaat gtccgataaa atcgctaaac tacgggaacg
     1201 tggtattcaa aaagagacac ttcgtacgat gattattgga attccgaatg caggcaaatc
     1261 gactctaatg aatcgcctag caggtaaaaa aattgcagtg gttggtaata aaccaggtgt
     1321 cacaaaaggt cagcaatggc tcaaatctaa taaggaactg gaaattttgg acactcctgg
     1381 tatcttgtgg cccaaatttg aagatgaatt agttggatta aagctagcat taacaggggc
     1441 aatcaaagat caattattgc ctatggatga ggtaacaata tttggattga attactttaa
     1501 aacctattat cctgatcgtc tcaaagagcg ttttaaaagt attaatttag aggatgaatc
     1561 tcctgaaatc atcatgacct taacacaaaa attaggttat cgtgacgatt acgatcgctt
     1621 ttataattta tttgtcaaag aagttcgcga tggtaaatta ggacgctata cgttagatat
     1681 cgtcggagaa catgatggca acaattaaag aaatcaaagc aattttagag actattgttg
     1741 atttaaaaga caaacgttgg caagaatatc aaacagattc acgcgcaggt gttcaaaaag
     1801 caatccttca acgaaaaaaa aatattcagt cacatttaga cgaagaagcg cgcttagaac
     1861 aaatgttggt ctacgaaaaa aaactttaca ttgaacatat aaaccttatt gcaggaatcg
     1921 atgaagtagg tagaggtcct ttagcaggac cagtagtagc tgctgctgtt attcttccac
     1981 caaactgtaa aataaaacat ctcaatgact ccaaaaaaat tccaaagaaa aagcatcaag
     2041 aaatctatca aaatatttta gatcaagcgt tagcagttgg tattggtatt caagacagcc
     2101 aatgtattga tgatatcaac atttatgaag ctacaaaaca tgctatgatt gatgcagttt
     2161 ctcatttatc tgtagttcct gaacatcttt taattgatgc tatggttttg gatctaccaa
     2221 ttccacaaac taaaattatc aaaggtgatg ctaattcact ctctattgca gcagcttcaa
     2281 ttgtagctaa agtgaccaga gataaaataa tgtctgatta tgatagcaaa tatcctggat
     2341 ttgcattttc taaaaatgct ggctatggta ctaaagagca tttagagggt ttgcaaaaat
     2401 acggtattac tccgatacat cgtaaaagtt ttgaacctat aaagtcaatg ttgtaaataa
     2461 tacggaagag gaaaagatga ccgaaaaaga aaaaatgtta gcaggacaat attatcgtcc
     2521 atcagcacct gaactacgca aggatagaga agttgcacta aaaaatatgc aagcctttaa
     2581 caatgaagat gatagtagca aacgtaacgt cattttacaa aaatggtttg gtgctactgg
     2641 gaagagtatc catatggaac aacgttttgt ctgcgattat ggttgtaata tttatgttgg
     2701 tgaaaacttc tacgccaatt ttaaccaaac tttcttagat gtttgtgaaa ttcgtattgg
     2761 cgataactgt atgtttggcc caaactgtca actcctcacc ccactccatc ctctagatcc
     2821 aatagaaaga aattcaggat tagaatacgg tgccccaatc caaatcggga ataatgtctg
     2881 gctaggtgga ggagtgacta ttttaccagg tgttgtatta ggagataatg tcgtcgttgg
     2941 cgctggaagt gtcgttacga aatcatttga aaataatgtt gttatagcag gaaaccctgc
     3001 taagataatc aaaaaattag cttaatgtaa gaaaaaacct ttaattataa caaaggtttt
     3061 ttctaattta aaaatagata aagagaatat tctgttaatc taatctatct tatttataat
     3121 gattttaaat
//
LOCUS       DATROQ010000141         3117 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.142, whole genome shotgun sequence.
ACCESSION   DATROQ010000141 DATROQ010000000
VERSION     DATROQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.142"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..959)
                     /locus_tag="VBC94_001954"
     CDS             complement(<1..959)
                     /locus_tag="VBC94_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEO6980189.1"
                     /translation="MVFAKISQLAHYAPSQIIKNEDLSLIMDTSDDWISSRTGIKQRH
                     ISKNETTADLANKVAEQLIEKSGYSASQIDFIIVATMTPDSMMPSTAARVQAHIGASN
                     AFAFDLSAACSGFVFALSTAEKLISSGSYQKGLVIGAETVSKVLDWTDRGTAVLFGDG
                     AGGVLLEASKEKHFLAESLNTDGSRQGLQSSQVGLNSPFSDEVLDDKFLKMDGRAIFD
                     FAIKEVSKSINHLIETSHLEKEDIDYLFLHQANRRILDKMSRKIDIARDKFPENMMDY
                     GNTSAASIPILLSESYENGLLKLDGNQTILLSGFGGGLTWGSL"
     gene            complement(959..1393)
                     /locus_tag="VBC94_001955"
     CDS             complement(959..1393)
                     /locus_tag="VBC94_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6980190.1"
                     /translation="MEYDQINSYLVDIFNRIMIIEEMSLKTSQFSDVSLKEMHTIEII
                     GKHSEVTPSDVARELMLTLGTVTTSLNKLEKKGYIERKRSSIDRRVVHLSLTKRGRLL
                     DRLHSKFHKSMVSHIIEDLGEEDIKMLTSALGNLHKFLEDLV"
     gene            complement(1489..2280)
                     /locus_tag="VBC94_001956"
     CDS             complement(1489..2280)
                     /locus_tag="VBC94_001956"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEO6980191.1"
                     /translation="MKFENIIYGIDGSVATIMLNRPDISNGFNIPMCQEIIDAIRLMS
                     ENKDVMFLVIEAQGPIFSIGGDLKVMKDAVESDDISSLTKIAELVNQISYDLLQLEKP
                     VVMCVDGAVAGAAANIALAADFVIASKKSKFIQAFVGVGLAPDAGGLLLLSKSIGITR
                     AVQLALTGESLSAEKAEALGIVYKLCESDKIGKIKDQLLKRLSRHSINSYQAIKSLAW
                     EAAFKDWEQYKKLELQLQESLAFKQDFKEGVRAHADRRRPNFLGK"
     gene            complement(2418..3068)
                     /locus_tag="VBC94_001957"
     CDS             complement(2418..3068)
                     /locus_tag="VBC94_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001900118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO6980192.1"
                     /translation="MCKYKAIIFDMDGVLFDTELFYYKRRERFLKQHGITINHLPMNF
                     FIGGNMKQVWKSVLGDQYDTWDIDKLQQDYSRYKEDNPLPYKDLIFQDCKRVIEKLHH
                     KGYLLGLASSSTRHDIMLALESFNLDTYFKVILSGEEFSESKPNPAIYNRAAELLDIP
                     KQQILIVEDSEKGIAAGIAAGIDVWAIEDKYFGMNQSQANVLVSDLSQFFVKLDNY"
BASE COUNT         1021 a          633 c          423 g         1040 t
ORIGIN      
        1 attagacttc cccatgtcaa acctccaccg aaacccgata agaggattgt ttggttacca
       61 tctagtttca acaaaccgtt ctcataagat tctgaaagta aaatagggat actagctgca
      121 ctagtattac cataatccat catattctct ggaaatttat cacgtgctat atcaattttt
      181 cgagacatct tatctaaaat ccttcgattc gcttggtgta agaaaagata atcaatatct
      241 tctttttcca aatgggatgt ttctataaga tgattgatac tttttgaaac ttctttgata
      301 gcaaaatcaa agattgctct accatccatt ttcaaaaatt tatcatcaag tacttcatct
      361 gagaaaggtg aatttaaccc gacttgacta gattgaagtc cctgacgact accatctgta
      421 ttgagagact ctgcaagaaa atgtttttcc ttagaagctt ctagcaagac acctccagct
      481 ccatctccaa ataaaactgc tgtccctcta tcagtccagt ctaatacttt agaaacagtt
      541 tctgctccta ttactaaacc tttttgatag ctacctgatg atatcaattt ttcggcggta
      601 gacaaggcaa aaacaaaacc actacatgct gcactcaagt caaaggcaaa agcattactc
      661 gctccaatat gagcttgcac tcttgcggca gtagaaggca tcatagagtc tggtgtcatc
      721 gttgcaacaa taataaagtc aatttgacta gcggaataac cggatttttc aataagttgt
      781 tctgcgacct tatttgccaa atcagctgtt gtttcatttt tactaatatg acgttgtttt
      841 ataccagtac gtgacgaaat ccagtcatca ctggtatcca tgattaatga taaatcctca
      901 tttttaatga tttgagaagg tgcataatgt gcaagttgac taattttagc aaaaaccact
      961 agactaagtc ctccaaaaat ttatgaagat tccctaaagc gctcgtcaac atttttatgt
     1021 cttcttcgcc taggtcttca ataatatgag ataccataga tttatgaaat ttactatgca
     1081 atcgatctaa taacctacct ctcttagtaa gactcaagtg aacaactcga cgatcaatac
     1141 ttgaccgttt acgctcaatg tagccttttt tttctaactt attaaggctg gttgttacag
     1201 ttcccaaagt taacattaat tctcgagcga catcgctagg agttacctca gaatgtttcc
     1261 caataatttc aatagtatgc atttctttta aagatacatc gctaaattgg ctagtcttta
     1321 aactcatttc ctcaataatc atgattctat taaaaatgtc aactaaataa ctattaatct
     1381 gatcgtattc caagatatca ccacctcaca ttaaactttg atagtcaaat tatatcaaag
     1441 ttcaaactta tttgcaagac tttaatctta aataatttga ttttcaaact atttgcctaa
     1501 aaaattagga cgccttctat cagcgtgtgc ccgaacaccc tctttaaaat cttgcttaaa
     1561 ggctaatgat tcttgtaact gtaattcaag ctttttatat tgttcccaat ctttaaaagc
     1621 agcttcccaa gctaatgatt ttatggcttg atatgagttt atagaatgtc tacttaaacg
     1681 ctttaatagt tgatccttaa ttttacctat tttatctgat tcacaaagtt tataaacaat
     1741 tcccaaagct tcagcttttt cagcagataa gctttctcca gttaaagcta gttgcactgc
     1801 acgagttata ccgattgatt tactaagtaa caataaacct cctgcatctg gagctaaacc
     1861 gacacccaca aaagcctgga taaatttact cttcttgctt gcgataacaa aatcagctgc
     1921 taatgcaata ttagctgcgg caccagctac agccccatct acacacataa caacaggttt
     1981 ctctagctgt agtagatcat acgatatctg attaactaac tcagcaattt tagttaatga
     2041 gctaatatca tcgctctcaa cagcatcttt catcactttt aaatctcctc cgattgaaaa
     2101 gattggtcct tgtgcttcaa tcactaaaaa catgacatct ttattttcag acataagcct
     2161 tattgcatca ataatttctt gacacattgg gatgttaaaa ccatttgaaa tatctggtcg
     2221 attaagcata atagtggcca cgctgccatc aataccatat attatatttt cgaacttcaa
     2281 aacgtctcct ctctttaaga gtgaaagaaa aataaaaaat aatttgatta tcaaattatc
     2341 atgctttgta ttttatcata aacctagctt tcatggcaag ataaaaaata aaaaaaacgg
     2401 aattaaacct cccgttttta atagttatct aatttcacaa aaaattgact cagatcgctc
     2461 actaatacat tagcctgcga ctgattcatt ccaaagtatt tgtcttcaat agcccacaca
     2521 tcaataccag ctgcaatgcc agctgcaatc cccttctcac tgtcttctac aataagaatt
     2581 tgctgtttag gaatatctaa aagttctgct gctctattat aaatagctgg attaggttta
     2641 ctttctgaaa attcctcacc agacagaatt accttaaaat atgtatccag attaaaactt
     2701 tctaatgcta acatgatatc atgtctagta gaacttgaag ctagacccaa aagatagcct
     2761 ttgtgatgaa gcttttcaat gactctttta caatcctgaa aaattaaatc tttgtatggc
     2821 aagggattat cctctttata gcgactataa tcttgttgga gcttatcaat atcccaggta
     2881 tcatattgat cacctaaaac agatttccaa acctgcttca tatttcctcc aataaaaaaa
     2941 ttcattggta aatggttaat tgtaattcca tgttgtttta aaaaacgctc tcgcctttta
     3001 taataaaata attcagtgtc aaatagaaca ccatccatat caaatattat tgctttatat
     3061 ttacacatgt aactattata tcattctttt aaatttagag gtaatgacaa attttct
//
LOCUS       DATROQ010000142         3063 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.143, whole genome shotgun sequence.
ACCESSION   DATROQ010000142 DATROQ010000000
VERSION     DATROQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.143"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            9..473
                     /locus_tag="VBC94_001958"
     CDS             9..473
                     /locus_tag="VBC94_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEO6980193.1"
                     /translation="MAIKNTSDNIEEYIKSLLEQSGIAEIKRSNLADTFQVVPSQINY
                     VIKTRFTESRGYVVESKRGGGGYIRIAKVHFSDQHQLFGNMLSTIGERISEQVFDDLI
                     QLLFDEEIITEREGNLILATSGDDVLGEQASVIRARMLRKLLQRLDRKGFHL"
     gene            470..2917
                     /locus_tag="VBC94_001959"
     CDS             470..2917
                     /locus_tag="VBC94_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO6980194.1"
                     /translation="MSHYSIKLQEVFRLAQFQAARYESHYLESWHLLLAMVLVHDSVA
                     GLTFAEYESEVAIEEYEAATILALGRAPKEEITNYQFLEQSPALKKILKLAENISIVV
                     GAEDVGTEHVLLAMLVNKDLLATRILELVGFRGQDDGESVRMVDLRKALERHAGFTKD
                     DIKAIYELRNPKKAKSGASFSDMMKPPSTAGDLADFTRDLSQMAVDGEIEPVIGRDKE
                     ISRMVQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGNIPYELRDMRVLELDMMS
                     VVAGTRFRGDFEERMNQIIADIEEDGHIILFIDELHTIMGSGSGIDSTLDAANILKPA
                     LARGTLRTIGATTQEEYQKHIEKDAALSRRFAKVLVEEPNLEDAYEILLGLKPAYEAF
                     HNVTISDEAVMTAVKVAHRYLTSKNLPDSAIDLLDEASATVQMMIKKNAPSLLTEVDQ
                     AILDDDMKSASKALKASHKDKKHKPIAVTEDHIMATLSRLSGIPVEKLTQADSKKYLN
                     LEKELHKRVIGQDDAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKALA
                     EVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTEKVRNKPYSVLLFD
                     EVEKAHPDIFNVLLQVLDDGVLTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFGA
                     KDISHDYTAMQKRIMEELKKAYRPEFINRIDEKVVFHSLSQDNMREVVKIMVKPLILA
                     LKDKGMDLKFQPSALKHLAEDGYDIEMGARPLRRTIQTQVEDHLSELLLANQVKEGQV
                     IKIGVSKGKLKFDIAKS"
BASE COUNT          995 a          470 c          652 g          946 t
ORIGIN      
        1 gtgaatttgt ggccataaaa aatacttccg ataatattga agaatatata aaatcattat
       61 tagaacaatc aggaattgct gaaataaagc gttccaattt agctgatact tttcaagttg
      121 ttcctagtca gattaattat gtcattaaga cacggtttac tgaaagccgt ggttatgtgg
      181 ttgagagtaa acgaggtggt ggaggttaca tccgcattgc taaggttcat ttttctgacc
      241 aacaccaatt atttggaaat atgttatcaa cgattggcga gcgtatcagt gaacaagtct
      301 ttgatgattt gattcaatta ttattcgatg aagagataat tacagaacgt gaaggaaatt
      361 tgattctggc gacatcaggt gatgacgtct taggagagca agcctctgtt attagagctc
      421 gtatgctccg taaattatta caacgattag atagaaaagg attccattta tgagtcatta
      481 ttccataaaa ttacaagaag tattcagatt ggcgcaattt caagcagcac gctatgaaag
      541 tcactattta gaaagctggc atcttctttt agcaatggtt ttagttcatg attcagtagc
      601 tggtttgaca tttgctgaat atgagtctga agttgcaatt gaagagtacg aagcagcaac
      661 aattttagct ttaggtagag caccgaaaga agagattacc aattatcaat ttttagagca
      721 atcaccagct ttgaaaaaga tcttaaagtt agcagaaaat attagtatcg tagtaggagc
      781 tgaagatgtg ggtactgaac atgtcttgct tgctatgttg gttaataaag atttattagc
      841 cacacgtatc ttagaattag ttggctttag aggtcaagac gatggtgaat cagttcgtat
      901 ggttgattta cgtaaggctc tagaacgtca tgccggtttt acaaaagatg atatcaaagc
      961 aatttatgaa ttacgtaatc ctaaaaaagc aaagtcgggt gcttcttttt ctgatatgat
     1021 gaaaccacca agtactgctg gtgatttagc tgatttcaca cgtgatttat cacagatggc
     1081 ggttgatggg gaaattgaac ctgttattgg gcgtgataaa gaaatttcac gtatggtcca
     1141 agtcttaagt cgtaaaacaa agaataatcc tgtcttagtt ggagatgcgg gtgtcggtaa
     1201 aacagctttg gcgtacggtc tagcacaacg tattgctaat ggaaatattc cttatgagct
     1261 tagagatatg cgtgttctag agttagatat gatgagcgtt gttgctggaa ctcgttttcg
     1321 tggtgatttt gaggaacgta tgaatcaaat cattgcagat attgaagaag acggtcatat
     1381 tattcttttc attgatgaac tgcatactat tatgggttct ggctctggta ttgatagcac
     1441 acttgatgct gctaatattt taaaaccagc tttggctcga ggcactttac gcactattgg
     1501 agcaacaact caagaggaat atcaaaagca tattgaaaaa gatgctgctt tatctcgtcg
     1561 ctttgcaaaa gttttagtag aagaaccaaa tcttgaagac gcatatgaaa ttctacttgg
     1621 attgaagcca gcgtatgaag cctttcataa tgttaccatt tcagatgaag cagttatgac
     1681 tgctgtaaaa gtggctcatc gttatttgac aagtaagaat ttaccagact cagctataga
     1741 cttacttgat gaagcttctg ccactgtgca aatgatgatt aagaaaaatg ctccatcgct
     1801 tttaacagaa gttgaccaag ctattttaga tgatgatatg aaatctgctt ctaaagctct
     1861 aaaagcaagt cataaggata aaaagcataa gcctattgcc gtaaccgaag atcatattat
     1921 ggcaactctc agccgtctat ctggtattcc tgttgaaaaa ttgacacaag ctgatagtaa
     1981 aaaatacctc aatttagaaa aagaattgca taaacgtgtt atcggtcaag atgatgcagt
     2041 tactgccatt agtcgtgcta tccgtcgcaa tcaatctggc attcgtactg gtaagcgtcc
     2101 tattggttcc tttatgttct tgggaccaac tggtgttggt aagacagagt tagcaaaagc
     2161 cttggcagaa gtgttgtttg atgatgaatc agccctaatt cgttttgata tgtcggaata
     2221 tatggaaaaa ttcgcagctt ctcgcctgaa tggggcacct ccaggttatg ttggttatga
     2281 tgaaggagga gagctgactg aaaaagtacg caataaacca tattcagttt tactttttga
     2341 tgaagttgaa aaagctcatc cagatatttt taatgtatta ttacaagtgc ttgatgatgg
     2401 cgttttaaca gatagtcgag gacgaaaagt tgacttttca aatactataa tcattatgac
     2461 ttctaattta ggagcaactg cattacgtga cgataaaaca gttggttttg gagctaagga
     2521 tatcagccat gattatactg ctatgcaaaa acgtattatg gaggagctta aaaaagctta
     2581 tcgaccagag tttatcaatc gtattgatga gaaagttgta ttccatagtc tcagtcaaga
     2641 taatatgcgt gaagttgtga aaattatggt taaaccttta attttagcgc ttaaagataa
     2701 aggtatggat ctgaaattcc agccttcagc cttgaaacac ttggcagagg atggctatga
     2761 tatagaaatg ggtgctcgtc cactgcgtag aactattcaa acgcaagtag aggatcattt
     2821 atcagaacta ctattggcaa atcaagtgaa agaggggcaa gtaattaaga ttggcgtatc
     2881 caaaggcaaa ttgaaatttg atattgcaaa atcataaaac attccagtac cgatgggtac
     2941 tggaatttta atataaaaag aaaatgtcca aaatattttg gacattttct tatagattta
     3001 tgaaaagtaa aaggatctat atctaacaat tgcacttgta gatataggta cgactgaaag
     3061 tcg
//
LOCUS       DATROQ010000143         3044 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.144, whole genome shotgun sequence.
ACCESSION   DATROQ010000143 DATROQ010000000
VERSION     DATROQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.144"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            95..168
                     /locus_tag="VBC94_001960"
     tRNA            95..168
                     /locus_tag="VBC94_001960"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:129..131,aa:Met,seq:cat)
     gene            171..243
                     /locus_tag="VBC94_001961"
     tRNA            171..243
                     /locus_tag="VBC94_001961"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:204..206,aa:Phe,seq:gaa)
     gene            255..335
                     /locus_tag="VBC94_001962"
     tRNA            255..335
                     /locus_tag="VBC94_001962"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:289..291,aa:Tyr,seq:gta)
     gene            342..412
                     /locus_tag="VBC94_001963"
     tRNA            342..412
                     /locus_tag="VBC94_001963"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:374..376,aa:Trp,seq:cca)
     gene            427..499
                     /locus_tag="VBC94_001964"
     tRNA            427..499
                     /locus_tag="VBC94_001964"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:460..462,aa:His,seq:gtg)
     gene            506..577
                     /locus_tag="VBC94_001965"
     tRNA            506..577
                     /locus_tag="VBC94_001965"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:538..540,aa:Gln,seq:ttg)
     gene            588..671
                     /locus_tag="VBC94_001966"
     tRNA            588..671
                     /locus_tag="VBC94_001966"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Leu,seq:caa)
     gene            1047..1347
                     /locus_tag="VBC94_001967"
                     /pseudo
     CDS             1047..1347
                     /locus_tag="VBC94_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865753.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1587..1790
                     /locus_tag="VBC94_001968"
     CDS             1587..1790
                     /locus_tag="VBC94_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980195.1"
                     /translation="MAESANTVRSNSFVLEELTEFQREKLKNGESVKFNFSKDVKKGN
                     QITQGGSNLPNDGSVYLQPNKSL"
     gene            1941..2381
                     /locus_tag="VBC94_001969"
     CDS             1941..2381
                     /locus_tag="VBC94_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein BUG3"
                     /protein_id="HEO6980196.1"
                     /translation="MKKHIKSIIPIVLIGMILGGCQMNSEHKSQYNETKSSKQSEVKK
                     DKKMTKKEQLAYLKEHEQEIIDFVKSQNKKIESVQIDWNDVRWSKGGNGTPQGGGEGI
                     LLFGEINNDSESSWRVDIDIEKGRLDLKNMYLGQPIRIGGKLFE"
     gene            2374..2718
                     /locus_tag="VBC94_001970"
     CDS             2374..2718
                     /locus_tag="VBC94_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980197.1"
                     /translation="MSNWNLESFDNLFATLAESTYTKRPSNFTYNELNPKLLRQLNSG
                     SSVELDFSKSVKKGKKVIEGGQNLPNDGIVYLQPDKSLKSIDEKVEVRVPDVNGGYHS
                     EHCVTNSYQKGY"
BASE COUNT         1055 a          442 c          615 g          932 t
ORIGIN      
        1 ttacccaagt ccggctgaag ggaacggtct tgaaaaccgt caggcgtgta aaagcgtgcg
       61 tgggttcgaa tcccacatcc tcctttaaag atatcgcggg atggagcagc taggtagctc
      121 gtcgggctca taacccgaag gtcgtaggtt caaatcctgc tcccgcaata ggctcggtag
      181 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cggttcgatt ccgtctcgcg
      241 ccatttatac cttaggagag atagcgaaga ggctaaacgc ggcggactgt aaatccgctc
      301 cttcgggttc gggggttcga atccctctct ctccatagta aacgggcata gtttaaaggt
      361 agaactaagg tctccaaaac cttcagtgtg ggttcgattc ctactgcccg tgttttttct
      421 aatatggcgg gtgtggtgaa gtggttaaca catcagattg tggctctgac attcgtgggt
      481 tcgattccca tcactcgcct tttcatgggg tatagccaag cggtaaggca agggactttg
      541 actccctcat gcgttggttc gaatccagct accccagttt taagatagcc ggcgtggcgg
      601 aattggcaga cgcgctggac tcaaaatcca gtgtcctcac ggacgtgccg gttcgacccc
      661 ggccgccggt atactttaaa agacaaggtt tctaaacctt gtcttttttt gttttgtact
      721 ggtaatttta agaaaaagtt actgaaaatt acttatattt gtttttcatt attgcaattg
      781 ttattgaagt tttaaaacaa gcagaaataa taaggaaatt tttcaggagt tagtggagac
      841 tcaacattta aatgaaattc aaacaggact cctaaatgtc ataggttctg aagtaataaa
      901 agtctttgtg aatgatagat aggtagtata aaataataag atttttcagt gtatatgtta
      961 cgactaaaaa attaaactcg tgaaaatttg ttatactaat attagtttta aaatactggt
     1021 aaataataat tatttttatt agatacttga catgggaagt ttattttcta agaaaagaga
     1081 gcatgatgaa aaaatatgta aaatggttaa ttcccatatc tattattggg atgttactag
     1141 caggttgtca aatgaatagt gaatctaaaa gtcagtcaaa cgaagtaaaa aatagcaaac
     1201 agtcagcagt aaagaaaggt aaaaaagtaa taaaaaaaga acaagtagct tatctcaaaa
     1261 agtatgaaaa agaattaact gaatatgtca aaacccaaaa tccagatatt agcgaaatta
     1321 agtatgattg gaatagtgtg cgtatagtgg aagctagtaa tggaacccct caaggcggag
     1381 agcagctatt attaatttgg ggttatgcaa atggcagtga attaaccaat tttactctaa
     1441 atttcacatt gggtgataat caaatgccta atattgactc agttggctca gataatttat
     1501 caactattga agattaataa taacagtaag aaaggtatat aatgggatgt tggaatctta
     1561 aggaatttga agatatattt tgaagtatgg cagaaagtgc taatacagtt cgatcgaatt
     1621 cttttgtttt agaagaatta actgaatttc aaagagaaaa gttaaaaaat ggggagtcag
     1681 ttaagtttaa tttttctaaa gatgttaaga aaggaaatca aatcacccaa ggtggctcaa
     1741 atctcccaaa tgatgggagt gtctatcttc agcctaataa gtccttataa actgtttatt
     1801 aaactgaaaa aattgaagtt ttcatagata aaattcttcg agtaaaatat tagagatgct
     1861 atttatattt cttaagctag taatttttta ttgtttattg gtttaatgat aatacaaata
     1921 taaaataggt ggtgcaaata atgaaaaaac atataaaaag tatcatacca atagttctta
     1981 ttggtatgat actaggaggc tgtcaaatga atagtgaaca taaaagtcag tataatgaaa
     2041 caaaaagtag caagcaatca gaagtgaaga aagataaaaa aatgacaaaa aaagaacaat
     2101 tagcttatct caaagagcat gaacaagaaa taattgattt tgtaaaatct cagaataaaa
     2161 agatagaatc tgtacaaatt gattggaatg atgttcgatg gagtaaaggg ggaaatggta
     2221 cacctcaagg aggaggagag gggattttac tttttgggga gattaataat gattctgaat
     2281 caagttggag agttgatatt gatatagaaa aaggacggct agacctaaaa aatatgtatt
     2341 taggacaacc tatacgaatt ggaggtaaat tatttgagta attggaactt agaaagtttt
     2401 gataacctat ttgctacttt agcagaatca acatacacaa aaagaccaag taactttaca
     2461 tataatgaat tgaatcctaa gttgctacga caactgaact caggtagttc tgttgaattg
     2521 gatttctcaa aatctgttaa gaaaggaaaa aaagttatcg aaggcggtca gaacctcccc
     2581 aatgatggta ttgtctacct gcaaccagat aagtccttaa aatctattga tgaaaaagta
     2641 gaggttcgcg ttcctgatgt caatggcggt tatcactctg agcattgtgt tactaattcc
     2701 tatcagaaag gttactgaaa gatgatgaag cagtggctct ataatcacta aaagctcatc
     2761 attgctcagt gtttaagtta acttctaaag ctcgaacctt gttaaatcaa ggtttgttta
     2821 cttttaaaag tgacatttta tttttctaaa aataaggtta tcgggattcc tcattctaag
     2881 ttactttgtt tatatgtacc tttacttaag gtttattttc attattttta atttaatatt
     2941 ttggagtatg tacgctccat tatagtattt tcgaagacat ctttaaaaga actgtagaag
     3001 ttaaaatatt ttaacctagt atctaacgtc atatattggt acat
//
LOCUS       DATROQ010000144         3023 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.145, whole genome shotgun sequence.
ACCESSION   DATROQ010000144 DATROQ010000000
VERSION     DATROQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.145"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..1122)
                     /locus_tag="VBC94_001971"
     CDS             complement(<1..1122)
                     /locus_tag="VBC94_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO6980198.1"
                     /translation="MHIIIQITIILLASVLATLISKRIGIPAVVGQLLVGIIIGPAML
                     GLVHQNQVLHVLSEIGVILLMFLAGLEANFDLLKKYLKPSLLVAITGVIVPMALFYFL
                     TRLFGFQINTAIFYGLVFAATSISITVEVLQEYNRVKTDTGAIILGAAVADDVLAVLL
                     LSVFIATNGSSSNIGLQIIIQLLFFVFLFICMKYLVPALFKLIEKVHFFEKYTILAIL
                     ICFSLSILADKVGMSSIIGSFFAGLAIGQTSFVDKVEHKISLLSYTFFIPIFFASIAL
                     PLKFDGMMSHLHTILIFTALAVLSKLIPGYFVGRGFNFSKLESLTIGGGMVSRGEMAL
                     IIVQVGLAAKIISSTTYSELVIVVILSTIIAPFILKYSFK"
     gene            complement(1392..2954)
                     /gene="guaA"
                     /locus_tag="VBC94_001972"
     CDS             complement(1392..2954)
                     /gene="guaA"
                     /locus_tag="VBC94_001972"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEO6980199.1"
                     /translation="MTDISILNDIQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TADEIRDINPIGIVLSGGPNSVYADGAFGIDEEIFELGIPILGICYGMQLITHKLGGK
                     VLPAGEAGHREYGQSALRLRSESALFAGTPQEQLVLMSHGDAVTEIPEGFHLVGDSVD
                     CPFAAMENTEKQFYGIQFHPEVRHSVYGNDILKNFAVNICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQRAIGDQLTCIFVDHGLLRKNEGDQVMDMLG
                     GKFGLNIIRVDASKRFLDLLSGVEDPERKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGTALGMPDEV
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
BASE COUNT         1077 a          573 c          511 g          862 t
ORIGIN      
        1 tttaaatgaa tatttaagaa taaatggcgc aataatagtt gacagtataa caacaataac
       61 aagctcggaa taagtagtgc ttgaaataat tttagcagct aatccaactt gaactataat
      121 gagagccatc tcgccacgac tgaccattcc accaccaatt gttaaagatt ctaattttga
      181 aaaattaaag cctcgaccga caaagtaccc tggaattaat tttgataaaa cagccaaagc
      241 agtaaagatt aatatcgtgt gaaggtgaga catcattcca tcgaatttaa gaggtagtgc
      301 aattgaagcg aagaaaattg ggataaagaa ggtgtaacta agaagtgaaa ttttatgttc
      361 taccttatca acaaaagaag tttgtccaat agctaatcct gcaaaaaacg aaccaatgat
      421 agaactcatt ccaactttat cggctaaaat tgatagagaa aaacagataa ggatagcaag
      481 aatagtatat ttctcaaaga aatgaacctt ttcgattaat ttgaaaagag caggtacaag
      541 gtatttcata caaataaata agaaaacaaa gaataacaat tgaatgataa tttgtaatcc
      601 gatattagaa gagcttccat ttgttgcaat aaagacactc agaagcaaaa cagctaagac
      661 atcatcagca acagcagcac ctaaaataat agcaccagta tctgttttta cacgattgta
      721 ttcttgtaaa acttcaactg tgatagaaat actagttgca gcaaatacga gcccgtaaaa
      781 gatagcagta ttgatttgga acccaaacaa gcgtgtcaag aagtagaaga gagccatagg
      841 aacaataaca ccagtaattg cgaccagtag actgggctta agatacttct ttaaaagatc
      901 aaaattagct tcaagacccg ctaaaaacat gagtaagata acaccaattt ctgacaggac
      961 atgtaatacc tgattttgat gaacaagtcc tagcattgca ggtccgatga tgatacctac
     1021 aagtagttgt ccaacaacag ccggtattcc aattcttttt gaaataagag ttgcaagaac
     1081 gcttgccaac aaaatgattg taatttgaat gataatatgc attttttcct cctaatatta
     1141 ataaaatgat atcaatcata ttgtactgaa tatttagaat accatttttt aagcctaaaa
     1201 tctaggaata tttcttttct gtctttttca tattattatc aagaaaatgg aaaaatatac
     1261 tgaaaaatat tcaatttaag aataatgaac ttgtaactat cgaactacat aaaaatgata
     1321 ttttaagtgt aagtatccct tattcaagaa ataagagtaa aaaaacttag ctacttaaag
     1381 tagctaagtt tttattccca ctcaacagtt gcaggtggtt tacttgtaat atcataaaca
     1441 atacggttaa cgtgatcaac ttcgttaaca atacgtgtcg aaattttctt aagtacatcc
     1501 caaggaagtt gcgcaaagtc agctgtcatg ccatcaattg aagtaattgc acggatagca
     1561 attgtatagt cataagtacg accatctccc ataacaccga cagaacgtac gccagtatta
     1621 actgtgaaat actgccatac gtcacgatct agaccagctt tagcgatttc ttcacgaaga
     1681 atagcatctg attcgcggac tgtttcaagt ttttcttcgg taatttcacc cataacgcga
     1741 atagcaagac caggcccagg gaatggttga cgccatacaa cttcatcagg cattccaagt
     1801 gcagtaccaa gagcgcgaac ctcatctttg aataatgtgt taagtggctc aatcaattca
     1861 aattgcatat cttctggcaa tccaccaaca ttatggtgtg acttgattgt ttgggctgtt
     1921 tctgtcccag attcaataat atctgtgtag agggtacctt gagcaaggaa atcaacgcct
     1981 ttaagtttac ttgcctcatc atcaaaaacg tacacgaact catttccgat gatttttcgt
     2041 tttctctcgg gatcttcaac accagaaaga agatctagga agcgttttga agcatcaaca
     2101 cggataatat taagtccaaa tttaccacca agcatatcca taacttggtc accttcattt
     2161 ttacgtaata aaccatgatc tacaaaaata catgttaatt gatcaccgat agcacgttgt
     2221 aggagtactc cgacaacaga tgaatcaaca ccgccagata gtccaagaag tactttacga
     2281 tcgcctacag tttcacgtat ttttgcaatt tccatatcaa tgaagttatc cattgaccaa
     2341 tcaccacgtg caccacagat attaacagca aaatttttaa ggatatcatt gccatatact
     2401 gagtgacgaa cttcggggtg gaattggata ccataaaatt gtttttcagt attttccatt
     2461 gcagcaaatg gacagtcaac agagtcacca acgagatgga aaccctcagg gatttcagta
     2521 acagcatcac catggctcat caaaacaagt tgttcttgag gagtgccagc aaagagtgct
     2581 gattctgagc gtaggcgaag tgctgattga ccatactcac ggtgaccagc ttctcccgca
     2641 ggaagaacct taccaccgag tttatgggta atcagttgca taccataaca aattcctaag
     2701 attggaattc ctaattcaaa aatttcttca tcgattccaa aagcgccatc agcgtatacg
     2761 gaatttggac cacctgaaag tacgatacca ataggattaa tatcgcgaat ttcatcagcc
     2821 gtaattttat gtgattttaa ctcagaaaat acaccaaatt cacggatacg acgtgcgata
     2881 agctggttat attgactacc gtaatctaga acgataattt tttggatatc attcaatata
     2941 gaaatatcag tcattgtttc cctttctttg tagctaattt taaaaacagc tgtacctata
     3001 ccattttagc atattttata taa
//
LOCUS       DATROQ010000145         2978 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.146, whole genome shotgun sequence.
ACCESSION   DATROQ010000145 DATROQ010000000
VERSION     DATROQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.146"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            158..295
                     /locus_tag="VBC94_001973"
                     /pseudo
     CDS             158..295
                     /locus_tag="VBC94_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            429..1534
                     /locus_tag="VBC94_001974"
     CDS             join(429..705,705..1534)
                     /locus_tag="VBC94_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag2 family transposase"
                     /protein_id="HEO6980200.1"
                     /translation="MSRKVRRHFTDDFKQQIVDLYNVGRKRSSLIKVYELTPSTFDKW
                     VRQAKTTGSFKSVDNLTDEQRELIELRKHNKELEMQLDILKQAAVIMAPKREIITANK
                     DKYSISKMCRWLNMPHSSYYYQAVESVSETEFEETIKRIFLDSESRYGSRKIKICLNN
                     EGITLSRRRIRRIMKRLNLVSVYQKATFKPHSRGKNEAPIPNHLDRQFKQERPLQALV
                     TDLTYVRVGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMFH
                     SDRGKEFDNQLIDEILEAFGITRSLSQAGCPYDNAVAESTYRAFKIEFVYQETFQLLE
                     ELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA"
     gene            1758..2890
                     /locus_tag="VBC94_001975"
                     /pseudo
     CDS             1758..2890
                     /locus_tag="VBC94_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1055 a          563 c          548 g          812 t
ORIGIN      
        1 tacttactgc caagcaaata caagagttac aaaaacgtaa tgcgcagctt gaagaggaga
       61 accttatcct aaaaaaagca agtgccatat tcatgcaaaa cttaaagtaa gactcctcgc
      121 tgtctatcgg ttacgctttg aacacgccac aacaaccttg tgtcgtgttt tacgtgtcaa
      181 tcgctccact tactataaat ttctaaaaca taagccctca aaaagagaat tggataatca
      241 aatttataga aaacaaatac ttgagattta taccaaagcg aacaaaagac ttggtgtgaa
      301 gtctatcaag gtcattcttc aaagagacta tgacacaaaa atctctgaag gaagaatttg
      361 aataggttac actaaactag acagaaatta taaagtgttc tacacttaag aaaaactgga
      421 gaaaaaccat gtctagaaaa gtacgtcgtc acttcaccga tgattttaag caacaaattg
      481 ttgaccttta caatgttggt agaaagcgta gcagtctcat caaggtatat gagctaaccc
      541 cttccacctt cgataagtgg gttagacaag ccaaaacaac tggttcattc aagtctgttg
      601 ataatctgac agatgaacag cgggagctaa ttgaactcag aaaacacaat aaagaactcg
      661 aaatgcaatt agatatccta aagcaagcag cggtgattat ggcacaaaaa gggaaataat
      721 cactgctaat aaggataaat acagcatttc aaagatgtgt cgctggctga atatgccaca
      781 ctcaagttat tactatcaag ccgtggagtc agtatctgaa acggagtttg aagaaactat
      841 taaaagaatt ttcctcgata gcgagtccag atacggatcc agaaaaatca aaatatgctt
      901 gaataacgaa ggtatcacac tttcacgtcg tcggattcga cgcattatga agcgactcaa
      961 tttggtttct gtttatcaga aagccacctt caaaccacat tctagaggca agaatgaagc
     1021 ccctattccc aaccacttag acaggcaatt taagcaagaa agaccactac aggccttagt
     1081 cactgactta acctatgttc gtgtaggcaa tcgttgggct tatgtttgcc tcatcattga
     1141 cctatacaac cgtgaaatca tcggcctgtc tcttggttgg cacaagaccg ctgaactcgt
     1201 taagcaagcc atacaaagca tcccttacgc cctgaccaaa gtcaagatgt tccattcaga
     1261 tcgtggcaaa gagtttgata atcagttaat tgatgaaata ttggaagcct ttggaatcac
     1321 acgttcgctt agtcaggctg gttgtcctta tgacaatgcc gtagctgaaa gtacgtatcg
     1381 tgctttcaaa attgaatttg tttatcaaga aacctttcaa ttgctggaag aactagctct
     1441 taagactaaa gactatgttc attggtggaa ttaccaccgc attcatggta gtctcaacta
     1501 ccaaacacca atgactaaaa gattaattgc ctagaaaaac actttataaa tgttgtacag
     1561 aaaagtgttg ccttttcact taataaaaga aagattgaag gaacacttct ctttcattcc
     1621 gaccaagggt cacaatttaa gtccggggaa tttagaaaaa taattgatga caacaatagg
     1681 cactgaatcc ctaaaatgag acaaaagaaa aacactcctg ttgaaattta gtaaactaga
     1741 aacaggagtg ttttattatg gttaaaaaag catattcatg ggaaactaag ctggcttgca
     1801 tcgatatgaa gaaagcaggc aagtctaaca gagttattat ggagacttta ggcatcaaaa
     1861 ataatagtca aatctatact tggatgaaat ggtacgaaaa cgaggagctt tatcgttttc
     1921 atcaaggtgt tggcaagcaa tatacctacg gtaaaggctt ggaacatctg tctgaagtgg
     1981 aacaattaca attacaggtt gacctcttaa aaaagtatcg aggcttgata aggaaatcga
     2041 taaagtaagt cttatcaagc ttgtggaaga ctataaaaac aactacccga tagttttcat
     2101 tctagactgt tttaaggtca agcggtcgac ctattaccgt tggaaaaagg agtatgagaa
     2161 gccgcaaaaa aagatgacct tattgaatta attgaacagc tctgtatgga gaatcacttt
     2221 atttatggct atcgtaccat tacacgattg ctcaaaaaaa tccacggatt gactgttaat
     2281 acaaagaaag tctatcgtat catgaaaaat aatggctggc tttgtcggac acgtaccaag
     2341 aaagttccca atttgggtaa agcttattat ttaacagata ataaattgag tcgggatttc
     2401 catgccgata agcctaagga aaagctcgta acagatatta cctacctata tttcggtaac
     2461 tgtaaattgt atctctcatc aattatggat ctctataacc gagaaattat agcttacact
     2521 atctcagatt gtcaggacac ggattttgta ctagacaccc ttaatcagtt aaaattgcct
     2581 aaataaagga gcaatcttac acagtgacca aggctcagtg tatacttcta tggcttacta
     2641 ccaggcttgc acggaaaaag acattatccg ctcaatgtct cgaaaaggaa cacctgcaga
     2701 taatgcctgt attgaatggt ttcacaccgt cctaaagact gaaacctttt atttccataa
     2761 taggagaaaa tacaacaaag atagtataac aaatattgtt aaaaattaca ttacatttta
     2821 taatgaaact agaattcaac agagattaaa cgaccagtcg cctgtacagt acagaaaact
     2881 gattgcctaa aagtgttttt cttgcgtctc actattaggg tgcagtgcca atatcatgca
     2941 ttctttttct aatcctggat atccttatga taatgccg
//
LOCUS       DATROQ010000146         2907 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.147, whole genome shotgun sequence.
ACCESSION   DATROQ010000146 DATROQ010000000
VERSION     DATROQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.147"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(104..2383)
                     /gene="pcrA"
                     /locus_tag="VBC94_001976"
     CDS             complement(104..2383)
                     /gene="pcrA"
                     /locus_tag="VBC94_001976"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEO6980201.1"
                     /translation="MNPLIIGMNDKQAEAVQTTDGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKYVNPWNILAITFTNKAAREMRERAIALNPATQDTLIATFHSMCVRILRREADYI
                     GYNRNFTIVDPGEQRTLMKRIIKQLNLDTKKWNERSILGTISNAKNDLLDEIAYEKQA
                     GDMYTQVIAKCYKAYQEELRRSEAMDFDDLIMMTLRLFDQNKDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPQAKVVL
                     LEENYRSTKKILQAANNVINHNKNRRPKKLWTQNDEGEQIVYHRANNEQEEAVFVAST
                     IDNIVREQGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVIA
                     YLNILANTSDNISFERIVNEPKRGVGPGTLEKIRSFAYEQNMSLLDSSSNVMISPLKG
                     KAAQAVWDLANLILTLRSKLDSLTVTEITENLLDKTGYLEALQVQNTLESQARIENIE
                     EFLSVTKNFDDNPEITVEGETGLDRLSRFLNDLALIADTDDSATETAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRAIEDADELEEERRLAYVGITRAEQILFLTNANTRTLF
                     GKTSYNRPTRFIREIDDELIQHQGLARPVNSSFGVKYSKEQPTQFGQGMSLQQALQAR
                     KSNSQPQVTAQLQALNANNSHETSWEIGDVATHKKWGDGTVLEVSGSGKTQELKINFP
                     GIGLKKLLASVAPISKKEN"
     gene            complement(2489..2875)
                     /locus_tag="VBC94_001977"
     CDS             complement(2489..2875)
                     /locus_tag="VBC94_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEO6980202.1"
                     /translation="MKFNLEQVKVFENYEIENWEEGQVTLTTKVVDSSLNYYGNAHGG
                     YLFTLCDQVSGLVAISTGYEAVTLQSNINYLRAGRLDDLLTVIGTCVHNGRTTKVVDV
                     HIKNQKDEMVTTAIFTMFVTGERKEK"
BASE COUNT          877 a          591 c          449 g          990 t
ORIGIN      
        1 agtaaaaacc aaatgataac ttattttgtc tattcaaatg atgcaaaaag actaagaata
       61 caattcttag tctctgatta tttaagcata taatagaaga aagttagttt tcctttttac
      121 taataggagc gacactcgct aataatttct ttaacccaat acctggaaaa tttattttta
      181 actcttgagt ctttccgcta cctgaaactt caagcaccgt accgtccccc cattttttat
      241 gagttgcaac atcaccaatc tcccatgatg tctcatggct attgttcgcg ttaagagcct
      301 ggagttgggc agttacttga ggttgtgaat tacttttacg tgcctgaagc gcttgttgaa
      361 gactcattcc ttgaccaaat tgtgtaggct gttcttttga atatttcaca ccaaatgaag
      421 aattaactgg tcgagccaac ccttgatgtt gtatcagctc atcatcaatc tcacgtataa
      481 aacgggttgg tctattataa ctagttttac caaataaagt acgggtatta gcattggtaa
      541 ggaaaagtat ttgttctgct ctcgtaatcc cgacataagc taaacgacgc tcttcttcta
      601 attcatcagc atcctctatt gcacgcgata aaggaaatac tccttcttcc ataccgatta
      661 agaaaacaac tggaaattct agacccttgg cagcatgtaa tgtcattaaa gtgacttcag
      721 ccgtttcagt ggcgctatca tcagtatctg ctattaaagc taaatcattt aaaaaacgcg
      781 atagacgatc taaaccagtt tcaccctcta ctgtaatctc tgggttatcg tcaaaatttt
      841 tagtcactga taagaattct tcaatatttt caatacgcgc ttgactttct aatgtatttt
      901 gaacttgaag tgcttctaga tatcctgtct tatccagtag gttttctgta atctccgtta
      961 cagttaaact atctagctta ctacgtagag tcaaaatcag attagctaaa tcccaaacag
     1021 cttgagcagc cttccctttt aagggcgata tcatgacatt tgaagaggaa tcaagaagag
     1081 acatgttctg ttcataggca aatgaccgta ttttttctaa agttcctggc cccacccctc
     1141 ttttaggttc atttacaatc cgctcaaaag aaatattatc agaagtattt gcaaggatat
     1201 tgagataagc aataacatct cgaatttctt tacgactata gaattttgtt ccaccaacca
     1261 ttgtatatgg aatattggat tttaaaagtg cttcctcaat agtacgagat tgcgcattcg
     1321 tacgataaag aacggcaaaa tctttgaaat tttttccttg ttctcgaacg atgttatcaa
     1381 tagttgacgc tacaaaaacg gcttcttctt gctcgttgtt agctctatga tatacaattt
     1441 gctcaccttc atcattttga gtccataatt ttttaggacg gcgattttta ttatgattga
     1501 tcacattatt agcagcttga agtattttct tagttgatcg ataattttct tctaataata
     1561 caactttggc ttgcggatag tccttttcaa aatcaagaat attttgcata tcagctccac
     1621 gccatccata aatggattga tcggcatcac caacaacaca aatatttttg aaacgcgaag
     1681 ctaataactt aactaattgg tattgagcgt ggttagtatc ttgatactcg tctacatgga
     1741 tatattgata cctctgttgg taataggcca aaacatcttt attctgatca aataatcgaa
     1801 gtgtcatcat aatcaagtca tcgaaatcca tggcctcact tctacgtaat tcctcttgat
     1861 aagctttata gcatttagca attacctgtg tatacatatc tccggcttgc ttctcatatg
     1921 caatttcatc aagaaggtca tttttagcat tagagattgt acctaaaatt gaacgttcat
     1981 tccacttttt tgtgtccaaa tttaattgct taatgattcg cttcattaaa gtacgctgct
     2041 cacctggatc aacgattgta aagttacgat tatatccaat ataatcagct tcacgacgta
     2101 aaatacgaac acacatacta tgaaaagttg caatcaaagt atcttgggta gctggattca
     2161 aggcgatagc acgttcacgc atttcacgcg ctgctttatt agtaaaagta atcgctaaaa
     2221 tattccaagg attaacatat ttttcatcta ttaaataagc aatacgatga gtcagaacac
     2281 gtgtttttcc agagccagct cctgccataa tcaaaagcgg tccgtctgtc gtttgtaccg
     2341 cttctgcttg tttatcattc attccaatta ttaaaggatt catttctact cctaattttg
     2401 tcaaaattgc ctctctagta tatcattttt tcatcttttt cccaatagaa aaaggagtta
     2461 aacagacgct gtctaactcc tacctttttt acttctcttt tctctctcca gttacaaaca
     2521 ttgtaaaaat agcagttgta accatttcgt ctttctgatt tttaatgtga acatcaacga
     2581 cttttgtagt acgaccatta tgaacacaag taccgatgac agtaagcaga tcatctaatc
     2641 ggcctgccct caaatagttg atatttgatt gtagagttac cgcttcatat cctgttgaaa
     2701 tagcaactaa accactgact tgatcacaca gtgtaaacaa ataaccacca tgagcattac
     2761 cataatagtt gagcgaagag tcaacgacct tcgttgttaa ggtaacttga ccttcttccc
     2821 agttttcaat ttcgtaattc tcaaatactt ttacttgttc taaattgaat ttcattctac
     2881 tataacctca ctttttctat gaccatt
//
LOCUS       DATROQ010000147         2833 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.148, whole genome shotgun sequence.
ACCESSION   DATROQ010000147 DATROQ010000000
VERSION     DATROQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.148"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..1160)
                     /locus_tag="VBC94_001978"
     CDS             complement(<1..1160)
                     /locus_tag="VBC94_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEO6980203.1"
                     /translation="MIPFTIEYIFVLIGAFLLSIILTPIIRVISLKVGAVDKPNARRI
                     NKVPMPSSGGLAIFLSFVVTTLFFMPMAASRHFIEVSYFHYILPVIIGGLVVTTTGFI
                     DDIFELRPRYKMLGIIIAAIIIWKFTHFRFDSFKIPIGGPLLEFGPILTFFLTVLWII
                     SITNAINLIDGLDGLVSGVSIISLATMAVVSYFFLPKIDFFLTLTIVILIASIVGFFP
                     YNYHPAIIYLGDAGALFIGFMIGVLSLQGLKNSTAVAVITPVIILGVPILDTAVAIVR
                     RKLSGKKISEADKMHLHHRLLSMGFTHRGAVLVVYGIAIIFSLIALLLNVSSRIGGIF
                     LLLALLLVMEIFIEGLNIWGENRTPLFNLLKFIGNSDYRQSVIAKYSDKHQK"
     gene            complement(1162..1917)
                     /gene="mecA"
                     /locus_tag="VBC94_001979"
     CDS             complement(1162..1917)
                     /gene="mecA"
                     /locus_tag="VBC94_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEO6980204.1"
                     /translation="MEMKQISETTLKITISMEDLEDRGMELKDFLIPQEKTEEFFYSV
                     MDELDLPENFKNSGMLSFRVTPKKDRIDVFVTKSELSKDLNLEELADLGDISKMSPED
                     FFKTLEQSMLEKGDTDAHAKLAEIENMMDKATQEVVEENVSEEQPEKEVETIGYVHYV
                     FDFDNIEAVVRFSQTIDFPIEASELYKNGKGYHMTILLDLENQPSYFANLMYARMLEH
                     ANVGTKTRAYLKEHSIQLIHDDAISKLQMIEMG"
     gene            complement(2037..>2833)
                     /locus_tag="VBC94_001980"
     CDS             complement(2037..>2833)
                     /locus_tag="VBC94_001980"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEO6980205.1"
                     /translation="EGVTEWLPVSSTGHLILVQEFMKLNQSKSFVEMFNIVIQLGAIM
                     AVIVIYFKRLNPFQPGKSAREIRLTWQLWLKVVIACIPSILIALPFDNWFEAHFNFMI
                     PIAIALIFYGFVFIWVEKRNAHLKPQVTELASMSYKTAFLIGCFQVLSIVPGTSRSGA
                     TILGAIIIGTSRSVAADFTFFLAIPTMFGYSGLKAVKYFLDGNVLSLDQSLILLVASL
                     TAFVVSLYVIRFLTDYVKRHDFTIFGKYRIVLGSLLILYWLVVHLF"
BASE COUNT         1013 a          538 c          426 g          856 t
ORIGIN      
        1 tatttttgat gcttatcgct atatttagca ataacacttt gtctgtaatc actgttgcca
       61 atgaacttta agagattaaa tagcggtgtc cgattctcac cccaaatatt taaaccttct
      121 ataaaaattt ccattactaa tagtagagcc aatagaagaa aaattccccc aatacgacta
      181 gaaacattta aaagcaaagc gataagcgaa aagataattg caataccata aacaactaaa
      241 actgctcctc tatgtgtaaa tcccatagat agcaatcggt gatgcaggtg catcttatca
      301 gcttcagaaa tctttttccc tgataattta cgcctcacaa tagcaacagc tgtatccaaa
      361 ataggaactc ctaaaataat gacaggtgtt atcactgcaa cagctgtgga gttttttaaa
      421 ccttgaagcg ataatacacc aatcataaat cctataaaca aggcaccagc atcacctagg
      481 tagataatgg ctggatgata attataaggg aaaaaaccaa ctattgaggc gattaaaata
      541 acaatagtta gcgtgagaaa aaaatctatt ttaggtaaaa agaaataaga aaccactgcc
      601 atcgttgcta gactgatgat tgaaacacca ctaactagac catctagacc atcaatcaga
      661 ttgatagcat tagtgattga aataatccat aaaactgtca aaaagaaagt taaaatagga
      721 ccaaactcca agagtggacc acctattgga atcttaaaac tatcaaatct aaaatgtgta
      781 aatttccata tgataatagc tgcaataata attcccaaca ttttataacg aggtcttaat
      841 tcaaagatgt catctataaa tcctgttgtt gtaactacga gaccgccaat aattacaggc
      901 aaaatgtagt gaaaataaga gacttctata aagtgtctac tggccgccat tggcatgaaa
      961 aataatgttg ttacaacaaa ggagagaaat attgctaaac caccactact tggcattggt
     1021 actttgttga ttcttcgagc gttcggttta tcaaccgctc ctaccttcaa agatataact
     1081 cgaataatag gagttagaat gattgataat aaaaatgctc caatcaatac aaaaatatac
     1141 tcaattgtaa aaggtatcat tttatcccat ctcaatcatt tgtaattttg aaatagcatc
     1201 atcatgaatc aattgaatac tatgctcttt caagtaagct cgtgtttttg tgcccacatt
     1261 agcatgttca agcattcgag catacatcaa attagcaaag tagcttggtt ggttttccaa
     1321 gtctagcaaa attgtcatat gatagccttt accattttta tagagttcag aagcttctat
     1381 tggaaaatca atcgtttgtg aaaatcgaac tacagcttca atattatcaa aatcaaagac
     1441 atagtgaaca tatccaatcg tttctacttc cttttcaggt tgttcttcag aaacattttc
     1501 ctcaactact tcttgagttg ctttatccat catattttca atttctgcta atttggcatg
     1561 ggcatccgta tccccttttt ccaacatcga ttgttccaag gttttaaaaa agtcttctgg
     1621 agacattttt gaaatgtcac ccaaatctgc taattcttct aaatttaaat ctttacttaa
     1681 ttcagacttt gtaacaaaaa catcaatgcg atctttttta ggtgttactc gaaaacttaa
     1741 cataccacta tttttaaagt tttctggcaa gtctaattca tccatgacag aatagaaaaa
     1801 ttcctcagtc ttctcctgag ggattaggaa atctttcagc tccataccac gatcttctaa
     1861 atcttccata ctaattgtaa ttttcagtgt tgtttcactg atttgtttca tttccatatc
     1921 atcacctcat acgagtattc agttttctta ttataccgaa aaagctattt tttagcaatt
     1981 tttcacagcc taacacgcta aaaagctgag gaatactcag ctttttatgt tatattttag
     2041 aataaatgaa caactaacca gtagaggatg agtaaacttc ctaagactat acgatactta
     2101 ccaaagatgg tgaaatcgtg tcgtttgaca tagtctgtca agaaacgaat aacatataaa
     2161 ctaactacga aagctgtcag acttgctact aaaagtatta aagattggtc taaactcaag
     2221 acgttaccat ctaaaaaata tttaaccgcc ttaagtccac tataaccaaa catagttggg
     2281 atggcaagga agaaagtaaa gtcagcagcg accgaacgac tagttccaat aataattgct
     2341 cctaaaatag tagctccaga acgactggtt cccggaacaa tactgagaac ctggaaacat
     2401 ccaatcaaga aagctgtctt gtaagacata cttgccaatt cggttacctg tggtttgagg
     2461 tgtgcattac gtttttcaac ccatatgaag acaaaaccat aaaagattag ggctattgca
     2521 atcggaatca tgaaattaaa atgagcttca aaccaattat caaatggtaa agcaattaaa
     2581 atagatggaa tacaagctat cactactttt aaccataatt gccaagttaa acgaatttct
     2641 ctagcagatt tacctggctg aaatgggtta aggcgtttaa aataaatgac aataactgcc
     2701 ataattgccc caagttgtat aacaatatta aacatttcaa caaaggattt gctttggttc
     2761 aacttcataa actcttgaac caagattaaa tggccagtac tagaaacagg taaccattct
     2821 gtcactccct caa
//
LOCUS       DATROQ010000148         2826 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.149, whole genome shotgun sequence.
ACCESSION   DATROQ010000148 DATROQ010000000
VERSION     DATROQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.149"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..663
                     /locus_tag="VBC94_001981"
     CDS             <1..663
                     /locus_tag="VBC94_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6980206.1"
                     /translation="VKFVEKRPFSELGFSTKFKRTLWSLIKGWSIGFILFSISVITAY
                     ILGGLDFHSYDVSKATIFYVVTLLPFWLIQSGTEELLTRGWLLPLINHRFHLAVAIGV
                     SSTLFGILHLVNAHVTFLSIVSIICSGVLMSLYMIKSGDIWSVAALHGAWNFSQGNLY
                     GIAVSGQKAGASLLHFTVKENAPDWISGGAFGIEGSLISIFVLLAAIIYLLWLIKTEE
                     TD"
     gene            724..2715
                     /gene="uvrB"
                     /locus_tag="VBC94_001982"
     CDS             724..2715
                     /gene="uvrB"
                     /locus_tag="VBC94_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEO6980207.1"
                     /translation="MIDRKDTNRFKLVSKYSPSGDQPQAIETLVDNIEGGEKAQILKG
                     ATGTGKTYTMSQVIAQVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGQEISRDQLLNNLVDIQFERNDIDFQRGKFRVRGDVVEFFPASRDEHAFR
                     IEFFGDEIDRIREIESLTGRVLGEVEHLAIFPATHFMTNDEHMEEAISKIQAEMENQV
                     ELFEKEGKLIEAQRIRQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPFTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYEMEQTDTVVEQIIRPTGLLDPEVEVRPSMGQMDDLLGEINLRTEKG
                     ERTFITTLTKRMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSNGHVIMYADKITDSMQ
                     RAMDETARRRRLQMDYNEKHGIVPQTIKKEIRDLIAITKSNDSDKPEKVVDYSSLSKK
                     ERQAKIKALQKQMQDAAELLDFELAAQIRDVILELKAID"
BASE COUNT          897 a          438 c          595 g          896 t
ORIGIN      
        1 gtaaaattcg tagaaaaaag acctttttca gaattgggtt tttctacaaa gtttaaaaga
       61 actctgtggt cacttataaa aggctggagt ataggtttta ttctattttc aatttctgtc
      121 atcactgctt atatattggg aggacttgac tttcatagct atgatgtttc taaggcaacg
      181 attttctatg ttgtaactct ccttcctttt tggcttattc aaagcggtac ggaagaattg
      241 ctaacacgag ggtggcttct tcctctaatt aatcatcgtt ttcatttagc tgttgccata
      301 ggggtctcat ccactttatt tggcattttg cacttggtaa atgcccatgt cacttttctt
      361 tctattgtaa gtattatatg ttcgggagtt ttaatgtcct tgtatatgat taaaagtggt
      421 gatatttgga gcgttgcagc cttacatgga gcttggaatt ttagccaagg taatctctat
      481 ggtattgcag taagtggtca gaaagcagga gcatctcttt tgcattttac cgttaaagaa
      541 aacgcaccgg actggatatc aggtggtgca ttcggaatag aaggaagttt gatttctatt
      601 tttgttctct tagctgcaat tatatacctt ctatggctaa ttaagactga agaaacagat
      661 tgagtgttgc agctcagtct ttttcttttt tctatgttaa aataaagtaa tcgaggtaaa
      721 gaaatgatag atagaaaaga tactaaccga tttaaattag tttccaaata tagtccttca
      781 ggagatcaac ctcaagctat tgaaacgttg gttgacaata ttgaaggagg agaaaaagct
      841 cagattttaa aaggtgctac tggtactggt aaaacctaca caatgagcca agttatcgcc
      901 caagttaaca agccaacctt ggtgattgca cataataaaa ccctagcagg tcaactttat
      961 ggagagttta aagaattttt tcctgacaat gccgtagagt actttgtttc atattatgat
     1021 tattatcaac cagaagctta tgtgccttct tcggatactt atattgaaaa agatagctca
     1081 gtcaatgacg aaattgacaa attacgtcac tcagcgactt cttcgttatt agaaagaaat
     1141 gacgttattg ttgtagcatc tgtttcttgt atttacggtt taggttctcc taaagagtat
     1201 gcagacagtg ttgttagcct taggccaggt caagaaattt cacgtgatca gttattgaac
     1261 aatctcgttg atattcaatt tgagcgtaac gatattgatt ttcaacgtgg aaaatttcgc
     1321 gttcgtggcg atgttgtaga attttttcct gcgagtcgtg atgagcatgc ctttcgaatt
     1381 gaattttttg gtgatgaaat tgatcgtata agagaaatag aatcgttaac agggcgtgtg
     1441 ctaggagaag tggaacattt agctattttc ccagcaacac actttatgac aaatgatgaa
     1501 catatggaag aagctatttc aaaaatacaa gctgaaatgg aaaatcaagt agaacttttt
     1561 gaaaaagaag ggaaattgat tgaagcgcaa cgtattcgac aaaggacaga atatgatatt
     1621 gaaatgcttc gtgaaatggg atatacaaat ggtgttgaga actactcacg tcacatggat
     1681 ggtagaagtg aaggtgagcc accttttact ttgttagatt tcttcccaga agattttttg
     1741 attatgattg atgagagtca catgacaatg gggcaaatca aagggatgta taatggagac
     1801 cgttcgcgta aagaaatgtt ggttaattac ggttttcgtt taccatctgc cttagataac
     1861 cgtccacttc gtcgtgaaga attcgaaagc catgtccatc aaattgtcta tgtatctgca
     1921 acgccaggag actatgaaat ggagcaaaca gatacagttg ttgagcaaat tatccggcca
     1981 acaggacttc ttgatccaga agttgaagtt agaccaagta tggggcagat ggatgacttg
     2041 cttggtgaaa tcaatttgag gactgaaaaa ggtgagcgta cctttattac aacattgaca
     2101 aagcgtatgg cagaagactt aacagattat ctcaaagaaa tgggagttaa agttaagtat
     2161 atgcacagcg atattaaaac cttagaacgt acagagatta ttcgtgatct acgacttggt
     2221 gtgtttgatg ttttaattgg tattaatctc ttacgtgaag gaatagatgt tcctgaagtt
     2281 tcccttgttg caatcttaga tgctgataag gaaggtttcc ttcgtaatga gcgtggtctt
     2341 atccaaacaa tcggacgtgc agcacgtaac agtaatggtc atgttattat gtatgctgat
     2401 aaaataacag attctatgca aagagctatg gatgagaccg ctcgccgtcg tcgtttacaa
     2461 atggattata atgaaaagca tggaattgtg ccacaaacaa taaaaaaaga aatccgtgat
     2521 ttaattgcta ttactaaaag taatgatagc gataagcctg aaaaagttgt cgattacagt
     2581 agtttatcta aaaaagaaag gcaagctaaa attaaagctc ttcaaaagca aatgcaagat
     2641 gcagctgaat tattggactt tgagctagct gcacaaatcc gagatgttat cttagaacta
     2701 aaagcaatcg attaaactta tctctcatct ggttctctct ttaggagata gcagaaggtt
     2761 atagattatt aaataagctg actctagcag gtagagttag ctttttagtt tggttaaaaa
     2821 gaacta
//
LOCUS       DATROQ010000149         2800 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.150, whole genome shotgun sequence.
ACCESSION   DATROQ010000149 DATROQ010000000
VERSION     DATROQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.150"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            35..694
                     /locus_tag="VBC94_001983"
     CDS             35..694
                     /locus_tag="VBC94_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HEO6980208.1"
                     /translation="MSLEDYYLLSKKFSQVMTLDSHSSDDGYTIRSLYFDSLEDVDWQ
                     EKEDGVEIRRKIRLRNYGSQSNFAKLEMKQKQGANQKKRSLKLSKTDAQALINGNNSV
                     LLNYPDTFAAECYSRITTQLYQPKTVIEYQRKAFVAKENNIRITFDHHIIGTEASYDI
                     FSEDLNQNYLLDPYLVILEVKYNGFLLSYIKELLDSVDRSELSASKYCLGRAVSKHYR
                     F"
     gene            745..1422
                     /locus_tag="VBC94_001984"
     CDS             745..1422
                     /locus_tag="VBC94_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEO6980209.1"
                     /translation="MKDLLRNSLEQSGNLSFQDMILHILVAALLSVVIYVSYAYTHSG
                     TAYSKKFNVSLMTLTVLTATVMTVIGNNVALSLGMVGALSVVRFRTAIKDSRDTVYIF
                     WTIVVGICCGVGDYVVAALGSSVIFILLWVMGRVKNENRMLLIVKCDRTLEVDLEGIF
                     FQYFDGKAVQRVKNSTTNTIEMIFEISRKDYDKQLHVDNQLTEKVYQLGNIDYFNIVS
                     QSDEING"
     gene            1415..>2800
                     /locus_tag="VBC94_001985"
     CDS             1415..>2800
                     /locus_tag="VBC94_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEO6980210.1"
                     /translation="MGRLMRGKVIYGTTLIGLFLFLFFYFWIPKHHIERIHHHRIKQV
                     DAKSDLTGFKTHLPIISIDTKQQVIPLVTKEGGKYVKARDNINVDIELRDSPSRSHHL
                     SEKPRIRTKGLISYRGNSSRYFDKKSLKVKFVTNKLKEKKHRLAGMPKESEWVLHGPF
                     LDRTLLRNYLSYNIAGEIMSYAPNVRYCELFVNGEYQGVYLAVENIEQGEQRVPIEKS
                     DKKLHKTPYIVAWDREHKAKQKLDNYVHYTHQSGISALDVKYPGKQRLTSKQLEFINK
                     DINHIEKVLYSYDFSQYPKYIDRESFANYFVINEFFRNVDAGKFSTYLYKDLRDRAKL
                     VVWDFNNAFDNQIEGRVDEADFTLTDAPWFNMLIKDKAFIDLVVHRYKELRKGVLATE
                     YLSNYIDETRHFLDPAIDRNYKKWGYVFDLKNTDPRNYLIPTERNVTSYHKSVEQLKD
                     FIKKRGRWMDRN"
BASE COUNT          973 a          383 c          546 g          898 t
ORIGIN      
        1 tgaacgaagt tcttagacag gaaaaaaata tttaatatca cttgaagatt actatctttt
       61 atctaagaaa ttttcccaag taatgacgct cgattcgcat agttcagacg acggctatac
      121 tatccgttcg ctatactttg attctttaga agatgttgat tggcaagaaa aggaagatgg
      181 tgttgagata cgaagaaaga ttcgtttgcg caattatggt agtcaaagca attttgcgaa
      241 attagaaatg aaacaaaaac aaggtgctaa tcaaaaaaag cgctctctaa aattaagcaa
      301 gacagatgct caagcgctga ttaatggtaa caatagtgtt ttattaaatt atccggatac
      361 atttgctgcg gagtgttatt caaggataac aacgcaactt tatcaaccta aaacggttat
      421 tgagtaccaa agaaaagctt ttgttgctaa ggaaaacaat atccgaatca cctttgacca
      481 ccatataata ggtacagagg ctagttatga tattttctca gaggatttaa atcaaaatta
      541 tttattagat ccttatcttg ttattttaga agttaaatat aatggatttt tattgtctta
      601 tattaaagaa ctcttagatt cggtagatag aagtgaactt tcagcgagta agtattgttt
      661 aggaagagct gttagtaaac attatcgttt ttagtgaaaa agtatatacg ttaattatta
      721 ttaaatgtga gaggataaca aaaaatgaaa gacctattac gaaatagtct agagcaaagt
      781 ggaaatttaa gttttcaaga tatgatttta catattcttg tagcagcttt attgagtgta
      841 gttatttatg tttcctatgc ttatacgcat agtggaactg cctatagtaa aaagtttaat
      901 gtttcattaa tgacattgac ggtcttgact gcaacagtaa tgaccgttat tggtaataat
      961 gtagccttgt cattgggtat ggtcggtgcc ttgtcagttg ttcgttttag gacagccata
     1021 aaagattcaa gagatacagt ttatattttt tggaccatag ttgttggtat ctgttgtggt
     1081 gtcggtgact atgtggtagc tgcattagga agtagcgtta tctttatctt attatgggtt
     1141 atgggacgtg ttaaaaacga gaatcgtatg ttattgattg tgaagtgcga tagaacacta
     1201 gaagttgatt tagaaggaat tttcttccaa tattttgacg gaaaagctgt tcagcgtgtt
     1261 aaaaattcaa caactaatac tattgaaatg attttcgaaa tctctagaaa agattacgat
     1321 aagcaactcc atgtagataa tcagttaact gaaaaagtgt accaattggg aaatattgat
     1381 tatttcaaca ttgttagcca aagcgacgaa atcaatgggt agacttatga gagggaaggt
     1441 tatttacggc acaaccctta taggtctttt tctattctta tttttctatt tttggattcc
     1501 taagcatcac atcgagagaa tacatcatca tcgtataaag caggtagatg cgaagagtga
     1561 tttaacagga tttaaaaccc atttgcccat tatcagcatt gatacaaagc aacaagttat
     1621 tcctcttgtt acaaaagaag gcggaaaata tgtcaaagct agggataata ttaatgttga
     1681 tatcgaatta cgggattctc caagtagatc acatcattta tcagaaaagc cgagaattag
     1741 gacaaaaggg ttaatatcat atagaggaaa ttcctctcgt tactttgata agaagtcatt
     1801 gaaagttaag tttgttacta ataagttaaa ggaaaagaag catcgattag caggaatgcc
     1861 taaagaatcg gagtgggtat tgcatggtcc ctttctagac agaacattat taagaaatta
     1921 tctgagttat aatattgctg gtgagattat gtcctatgcc ccaaacgttc gctactgtga
     1981 gttatttgtc aatggtgagt atcagggagt atatctagca gttgagaata tcgaacaagg
     2041 tgagcaacga gttcccattg aaaaaagtga taaaaagttg cataaaacac cttatatcgt
     2101 tgcttgggat cgtgaacata aggcaaagca aaagttagat aactatgtac attatacaca
     2161 ccagtctggt atctcagctc ttgatgtgaa atatccaggt aaacaaagat tgacatcaaa
     2221 acaattagag tttattaata aggacatcaa tcatattgaa aaagtccttt attcttatga
     2281 tttttcacaa tatcctaaat atattgatag agaatcattt gcaaattatt ttgtcatcaa
     2341 tgaatttttt agaaatgtgg atgcgggtaa attttcaacc tatctatata aagatcttcg
     2401 tgatagagca aaactagtag tttgggattt taataatgct tttgataatc aaattgaagg
     2461 acgtgttgat gaggcagatt ttaccttgac ggatgcaccg tggttcaata tgctcattaa
     2521 ggacaaagca tttatagacc tggttgttca tcgctataaa gaattacgaa agggagttct
     2581 agcgacagaa tatttatcta attacataga cgagacaagg cattttttag atccagctat
     2641 tgatcgaaat tataaaaaat ggggctatgt ctttgattta aagaatactg atccaaggaa
     2701 ttatttgatt cctactgaac gcaatgtgac aagttatcac aaatcagtag aacaattaaa
     2761 agattttatc aaaaagcgtg gcagatggat ggatagaaat
//
LOCUS       DATROQ010000150         2790 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.151, whole genome shotgun sequence.
ACCESSION   DATROQ010000150 DATROQ010000000
VERSION     DATROQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.151"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..100
                     /locus_tag="VBC94_001986"
     CDS             <1..100
                     /locus_tag="VBC94_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome assembly protein"
                     /protein_id="HEO6980211.1"
                     /translation="ILTSKIEKIYQKHGITKEVLKTYYAKKTVRAD"
     gene            complement(190..2691)
                     /locus_tag="VBC94_001987"
     CDS             complement(190..2691)
                     /locus_tag="VBC94_001987"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEO6980212.1"
                     /translation="MTFYNHKEIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVAEYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFKLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQVLTASGESISYVPWPSYDESKLVENEIEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVVK"
BASE COUNT          751 a          619 c          602 g          818 t
ORIGIN      
        1 aatattaacg tcaaaaatag aaaaaatcta tcaaaaacat ggtattacaa aagaggtatt
       61 aaaaacttac tatgctaaaa aaacagtacg agctgactga tttagctaaa aatagaaaaa
      121 tcccaaaagt tcataaactt ttgggatttt atacgcgtaa atatcactat ttctagtaaa
      181 atctttatct tatttcacca caatattaac cagtttgttt ggtactgcga tgactttgat
      241 gatgtctttg cctgcgattt cggcttggac tttttcgtta gcaagggcaa cttcttgtaa
      301 ttcttcgcgt gaaaggtctt tagcaactac aagtttagct ttgaccttac ctttgatttg
      361 gacaacgatt tcaatttcgt tttcaaccaa tttgctttcg tcataacttg gccatggtac
      421 gtaagagatt gactcacctg aggctgtcaa gacttgccag agttcctcac ccaagtgtgg
      481 tgcgaatggt gcaatcaatt gaacaaagcc tttggcatag tcgctaaaga gtttgtcttc
      541 cttgttggct gcattgacaa agaccatgag ttgagcgata gctgtgttga atttcatttg
      601 gtcaacttgc tcagtaactg ccttaacggt ttcgttgtaa accttgtcaa gggctccgct
      661 attttcttca gtaatctctt tagtcgtgat caaacggtaa acacgatcca agaacttacg
      721 tgagccttca agaccttctt cagaccaagc gattgaagcg tcaagtggtc ccatgaacat
      781 ctcgtaaaca cgaagggtgt cagcaccgta ctgttcaaca acatcatctg ggttgacaac
      841 gttcttaagt gatttagaca tcttagctgg tgcttgttca agctcttccc cagtttccac
      901 atggaagaag ctaccatcac gtttttcaac cttatcagtt gccacaagag caccacggct
      961 atcgcggtag cttgttccca aaatcatccc ttggttaaag agtttttgga atggctcttt
     1021 ggtaggtacg acaccgagat cgtaaagaac cttgtgccag aagcgagcgt agagaaggtg
     1081 aagcacggcg tgctctgcac caccgacata gatatcaact ggcaaccatt gtttgaggag
     1141 ttcctcgtca gccaattttt cagtattgtg tggatcgatg taacggagat agtaccagct
     1201 tgaacctgcc cattgtggca tggtgttagt ctcacgacga cctttaacac catcttcacg
     1261 agtcacttcc aaccagtctg tcaagttagc aagtgggctc tcaccagtac ctgaaggacg
     1321 gatatcctta gttactggaa gaaccaatgg caattcactc tcaggaaccg ctgttgatgt
     1381 accatcttcc cagtggatga ttgggattgg ctcaccccag taacgttgac ggctgaagag
     1441 ccagtcgcgg agacggtaag tgactttctc attaccaaca ccttctgctt caagccattc
     1501 gaccattttg gcaatagctg acgctttatc gagaccatct aggaagtctg agttgatgtg
     1561 aagaccgtct tctgtaaagg cagcctcttc aacattacca ccttcaagaa ctggaatgat
     1621 gtctagctta aattgttttg cgaattccca gtcacgttca tcgtgggctg gaacagccat
     1681 gatagcacct gtaccgtagc tggcaaggac atagtccgca atccaaactg gaatttcttt
     1741 accattaact gggttaatgg cataggcacc tgtccagaca cctgtttttt ctttggcaag
     1801 gtctgtacga gcaaggtctg atttgagact tgcttgacgt ttgtattcgg caacggcttc
     1861 tgcttggtca gctgttgtaa tagcatcaac caaggcatgc tcaggagcaa gaactgcgta
     1921 agttgcacca aaaagggtgt caggacgtgt tgtaaagaca gtgaagtcct tatctgtatc
     1981 cttaaccttg aaggttacat tagcaccagt tgatttacca atccagttgc gttgcatgtc
     2041 tttgattgat tctggccagt caacttcttc caagtcttct agcaagcgct ctgcataggc
     2101 agtgattttg agcatccatt ggcgcattgg ttttctgaca actgggtagc caccacgttc
     2161 tgatgtacca tctggaagaa cctcttcgtt ggcgatagct gttcccaatt cttcaaccca
     2221 gttaactgga acttcagctt cgtaagctag acctttttca taaagcttgg taaagatcca
     2281 ctgtgtccac ttgtagtagt tagggtcagt tgtgttaact tcacgatccc agtcgtatga
     2341 gaaaccaaga gcattaatct ggcgtttgaa gttagcgatg ttttcagctg taaactcagc
     2401 tgggtcatta ccagtatcca tagcgtactg ctcagcagga agtccaaaag catcccaacc
     2461 cattgggtgg aggacattgt gaccttgggc acgtttgaaa cggctgagaa tatccgttgc
     2521 tgtatagcct tctgggtgac caacgtggag accagctcca gatgggtatg ggaacatatc
     2581 tagggcataa aacttaggtt ttgatgcgtc tgttcctgtt ttaaaggtgt gattatctgc
     2641 ccaaaaagct tgccatttgg gttcgatttc tttgtgatta tagaatgtca tcgcacttct
     2701 ccatgtcaaa aaagtatagt cctcttatta taacacgaaa aacagcaata aaaaaggtga
     2761 aaataccctc aacagcaggt tctctcccca
//
LOCUS       DATROQ010000151         2788 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.152, whole genome shotgun sequence.
ACCESSION   DATROQ010000151 DATROQ010000000
VERSION     DATROQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.152"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..473)
                     /locus_tag="VBC94_001988"
     CDS             complement(<1..473)
                     /locus_tag="VBC94_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5613 domain-containing protein"
                     /protein_id="HEO6980213.1"
                     /translation="MHYKELLESSEKAYLETNRYKHFKNIITSERYFSNYISYKIMPT
                     VSEFLQDMIYLADEQKDYQSDFAFVFFEENAELSSELQRTLSQQGFESEKHIIFTNSL
                     NHLKLFEKEFDDITFTKLNENYLEKYLKFKYKQSIVYGEIYAKQMYNDNRVNLLN"
     gene            complement(551..1885)
                     /gene="trmFO"
                     /locus_tag="VBC94_001989"
     CDS             complement(551..1885)
                     /gene="trmFO"
                     /locus_tag="VBC94_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEO6980214.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEAHRVPAGGAMAVDREGY
                     SEAVTEEIHKHPLIEVIRDEITDIPGDAITVIATGPLTSDSLAAKIHELNGGDGFYFY
                     DAAAPIVDKNTIDMNKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPEDYKGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLNQT
                     FATRKNPNLFFAGQMTGVEGYVESAASGLVAGINAVRRFNGESEVVFPQTTAIGALPH
                     YITHTDSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIATRALKDLEKFLNY"
     gene            complement(1999..2697)
                     /locus_tag="VBC94_001990"
     CDS             complement(1999..2697)
                     /locus_tag="VBC94_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6980215.1"
                     /translation="MLPAYIKIHDAIKKEIDKGTWKIGQRLPSERDLADDYSVSRMTL
                     RQSITLLVEEGILERRVGSGTYVASHRVQEKMRGTTSFTEIVNSQGRKPSSKLISFQR
                     KLANETEIQKLNLSQSDYVVRMERVRYADKVPLVYEVASIPENLIKGFEQSEVTEHFF
                     KTLTENGYEIGKSQQTIYARNASERVASHLEVNAGHAILALTQVSYFTDGKPFEYVHG
                     QYVGDRFEFYLENN"
BASE COUNT          866 a          554 c          416 g          952 t
ORIGIN      
        1 ttatttaaaa gattaacacg attatcatta tacatttgct tagcgtaaat ttctccgtaa
       61 actatacttt gtttatattt gaactttagg tatttttcta ggtaattttc attaagttta
      121 gtaaatgtga tgtcatcaaa ttctttttca aaaagtttaa gatgatttaa tgaatttgta
      181 aatatgatat gtttctctga ttcaaagcct tgttgtgata aagttctctg taattcactg
      241 ctcaattcag cattttcctc aaaaaataca aaagcaaaat cagactgata atctttttgt
      301 tcatctgcta aatagatcat atcctgtaaa aactcactta ctgtaggcat tattttgtag
      361 ctaatatagt ttgaaaaata acgctctgaa gtgattatat tcttaaaatg tttataccga
      421 ttagtttcca aataagcttt ttcggatgac tcaagtaatt ctttatagtg catatttact
      481 ccattcatac atttttaaaa atcaatatag aaaatttgac cttgcttgtt ggcaaagtca
      541 aatgaatata tcaatagttg agaaattttt ctaaatcttt caaagctcgt gtagcaatag
      601 cttcataacg ctctttctta tcacgaattc gtggaccttc taattctttg atgataccaa
      661 agttaacatt cattggttgg aaatgtttgc tgtctgtatg tgtaatataa tgtggcagtg
      721 caccaatagc agtagtttgt gggaaaacta cttcactctc cccattgaag cgacggacag
      781 cattgattcc cgctacaaga ccacttgctg ccgattctac ataaccttca acaccggtca
      841 tttgtccagc aaagaaaaga tttgggttct tacgtgtagc aaaggtttgg tttaaaagat
      901 ttggtgaatc catataagaa ttgcgatgca taacaccata ccggacaaat tcagcatttt
      961 caagaccagg aatcatttga aatacacgtt tttgttctcc ccacttaaga tgagtttgga
     1021 aaccaactat gttataaaga gagcctgcag cattatcttg acgtaattga acaacagcat
     1081 atggtgtttt aaattcacca tctcgcggtc ctttgtagtc ctcaggatac tccaaaccaa
     1141 ctggtttcat tggaccataa agcatcgttt taataccacg cttagccatc acttcgatag
     1201 gcatacaacc ttcgaagtat ttttctttct caaaagaatt tagtggtgct tcctcagctg
     1261 tagttaaagc ttcatgaaaa gccataaatt cttctttggt cattggacaa tttagataag
     1321 ctgcttcacc tttgtcatac ctagacttaa ggtaaacttt attcatgtca atcgtatttt
     1381 tatcgacaat tggagcggcg gcatcgtaaa aataaaaacc atcaccacca ttaagttcat
     1441 ggattttagc tgccagacta tcacttgtta atggtcctgt agcgataact gtaatagcat
     1501 ctccagggat atctgtgatt tcatcacgaa ttacttcgat taatggatgt ttatggattt
     1561 cctcagtaac agcttcagaa taaccttctc tatcaacggc catggctcca cctgctggaa
     1621 ctcgatgtgc ctccccattt ctcataatga tagaatccaa acgacgcatt tcttctttta
     1681 aaagaccaac tgcatttgtt agagaatcac cgcggaatga attggaacat actaattctg
     1741 caaaattatc tgttttatgt tgaggggttg atttaacccc acgcatttca taaagcttaa
     1801 ctggtattcc acgtttagca atttgataag ctgcttcaga accagccaaa ccagcgccga
     1861 taacattaat gtaagattga gacatgaact aatacctctt gaaagtagtc aactacttcc
     1921 tcaaatcttt ctatataatt tatttttcca ttataacata catagctact tcttcaaaaa
     1981 acgttattca ttaaactact agttattctc taaataaaac tcaaaacgat ctccaacata
     2041 ttgcccatga acatattcaa aaggcttacc atcagtaaag tatgaaactt gtgtcaatgc
     2101 caaaatagca tggcctgcgt taacttctaa atgcgaagca acacgttcac tagcatttct
     2161 agcatatata gtttgttggc ttttcccaat ttcgtagcca ttctctgtta aggttttaaa
     2221 gaaatgttct gtaacctctg attgttcaaa acctttaatt aaattctctg gtatagaggc
     2281 tacctcataa accaacggta ccttatcagc ataacgtacc ctttccatac gaaccacata
     2341 atctgactgt gataaattta atttttgaat ttcggtttca ttagctagtt ttctttgaaa
     2401 tgagattagt ttactggatg gcttacgccc ttgagaattg acaatttctg taaaactagt
     2461 tgttccccgc attttttctt gaacacgatg actagcaaca taggtgccac taccaactcg
     2521 tctctccaaa ataccttctt caactaacaa tgtaattgat tggcgaagtg tcatacgact
     2581 tacactataa tcatccgcta aatctctttc gctaggtaac cgctgcccaa tcttccaagt
     2641 ccctttatcg atttcctttt taatagcgtc gtgaatttta atatatgctg gtaacaatat
     2701 tctctcctcc tagaatctac acttcatttt accatacttc tatatatttc taactctaaa
     2761 atcgtgaaat aattcagaaa aatcataa
//
LOCUS       DATROQ010000152         2738 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.153, whole genome shotgun sequence.
ACCESSION   DATROQ010000152 DATROQ010000000
VERSION     DATROQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.153"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..967
                     /locus_tag="VBC94_001991"
     CDS             <1..967
                     /locus_tag="VBC94_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6980216.1"
                     /translation="TILVVGELFYNKSEKHLNLSEKQVVKIGILQYVTHDALDAIEKG
                     VEDGLAQEGYKGKKVKLTVLNAEADQSKIQAMSKQLVNHHNDILIGIATPSAQGLAAS
                     TKDTPIIMGAVSDPLGAKLVTNMKKPTTNVTGLSNVVPTKQTVQLIKDITPNVKRIGI
                     LYASSEDNSVSQVTEFTKYAQKAGLEVLKYSVPSTNEIKTSMSVMTKKVDAVFVPQDN
                     TIASAFRTVIVAANQANIPVYSSVDTMVEQGSIASVAQSQYGLGLETAKQAIKVLRGK
                     PVKDVPVKVIDTGKPSLNLKAAKHLGIKIPKKIMKQAEITVKVDD"
     gene            980..1843
                     /locus_tag="VBC94_001992"
     CDS             980..1843
                     /locus_tag="VBC94_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6980217.1"
                     /translation="MIISSVSQGLLWGILGLGIYLTFRILKFPDMTTEGSFPLGGAVC
                     VTLMNQGVNPILATILGMLSGMLAGFVTGLLYTKGKIPTILAGILVMTSCHSIMLMVM
                     KRANLGLNEIQTLKDFLPFSNDLNLLVLGLIAILLAISVLIYFLYTRLGQAYIATGDN
                     PDMAKSFGIDTDKMEMLGLIVSNGLIALSGALVSQQDGYADVSKGIGVIVIGLASIII
                     GEVLYSTGLTLFERLIAIVVGSILYQFLITAVIALGFNTNYLKLFSAIVLGICLMVPV
                     LKTKILKGVRL"
     gene            1843..2604
                     /locus_tag="VBC94_001993"
     CDS             1843..2604
                     /locus_tag="VBC94_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6980218.1"
                     /translation="MMMKIIELKEATVQVSNGLAEMKTILDHVNLSIYEHDFITILGG
                     NGAGKSTLFNVIAGTLMLSSGNIYIMGQDVTNLSAEKRAKYLSRVFQDPKMGTAPRMT
                     VAENLLVAKFRGEKRPLVPRKITNYTEEFQKLIARTGNGLDRHLETPTGLLSGGQRQA
                     LSLLMATLKKPNLLLLDEHTAALDPRTSVSLMGLTDEFIKQDSLTALMITHHMEDALK
                     YGNRVLVMKDGKIVRDLNQAQKNKMAIADYYQLFD"
BASE COUNT          943 a          405 c          554 g          836 t
ORIGIN      
        1 gaccatatta gtggttgggg aattatttta taacaaatca gaaaaacatc ttaatttatc
       61 agaaaagcaa gttgttaaaa ttggtatttt acagtatgta acccatgatg ctttggatgc
      121 tattgaaaag ggagttgaag atggtctagc tcaagaagga tataaaggga aaaaagttaa
      181 attaaccgtt ttaaacgccg aagctgatca aagtaaaatt caagcgatga gtaaacaatt
      241 agttaatcat cacaatgata ttttgattgg aattgcgaca ccatcagcac aaggtttagc
      301 agcttcgact aaggatactc ctataatcat gggagctgtt tctgatcctt taggggccaa
      361 gttagtaacg aatatgaaga aaccaacaac taatgttaca ggattatcaa acgttgttcc
      421 gaccaagcaa actgtgcaat taatcaagga tataactccc aatgttaaac gtataggaat
      481 tctttacgct agtagcgaag ataattctgt gtcacaagta actgagttta caaaatatgc
      541 tcaaaaagca gggctagaag ttcttaaata ttctgtacca tctacaaatg aaattaaaac
      601 aagtatgtca gtcatgacca aaaaagtaga cgctgtcttt gttccacaag ataatactat
      661 tgcgagtgct tttagaacag ttatcgtagc agcaaaccaa gctaatatac ctgtgtattc
      721 cagtgttgat accatggttg aacaaggaag tatagcatca gtagcgcaga gtcaatatgg
      781 cttagggtta gaaactgcaa aacaggccat taaagtccta agagggaagc cggttaaaga
      841 tgtaccagtc aaagttattg atacagggaa gccaagcctc aatttgaaag cagccaaaca
      901 cttaggaatt aaaattccta agaagattat gaaacaggca gaaattaccg ttaaggttga
      961 cgattagaaa gagggtttta tgattatttc atcagtttca caaggtttat tatgggggat
     1021 attaggatta gggatatact taacctttag aatcttaaaa tttccagaca tgacaacaga
     1081 gggctccttc cctctagggg gagcggtttg tgttacctta atgaatcaag gggttaatcc
     1141 tattttggcg acgattctag gcatgctatc agggatgtta gcgggatttg ttactggttt
     1201 actttatact aaaggaaaga taccgacgat tttagcaggg attttagtaa tgacctcatg
     1261 ccactctatt atgctaatgg ttatgaaaag agcaaatcta ggtttaaatg agattcaaac
     1321 attgaaagat tttcttccgt tttcaaatga tttaaatctt ctcgtattag gtttgatagc
     1381 tattctcttg gcgatttctg tcttaattta ttttctctat acacgcttgg ggcaagctta
     1441 tattgcaaca ggagacaacc ctgatatggc aaagagcttc ggcattgata ccgacaaaat
     1501 ggaaatgctt ggcttaattg tatcaaatgg cttaattgct ttatcaggag ctttagtaag
     1561 tcaacaagat ggatatgctg atgtatcaaa aggtataggt gttattgtta ttgggttagc
     1621 tagtattatt attggagagg tactttactc tactggttta actctctttg aacgtttaat
     1681 tgctatagtg gtgggttcta ttctttatca atttttaata acggcagtaa tcgccttagg
     1741 atttaatacg aattacctaa aattatttag tgcaatagtg cttggaattt gtttaatggt
     1801 tcctgtccta aaaacgaaga ttttgaaagg agtacgctta taatgatgat gaaaatcata
     1861 gagttaaaag aagcaaccgt acaagtcagt aatggtttag cagaaatgaa aacgatatta
     1921 gatcatgtta atttgagtat ttatgaacat gacttcatta caatattggg tggaaatgga
     1981 gctggaaaat caacgctttt taatgtaatt gcaggtacct tgatgttaag cagtggaaat
     2041 atttacatca tgggacaaga tgttactaat ttatcagcag aaaaacgagc taaatattta
     2101 tcacgggtat ttcaagatcc gaaaatggga acagcaccta ggatgacagt tgctgaaaat
     2161 ttattagttg ctaaatttcg aggtgaaaag agaccgttag ttcctagaaa gattactaac
     2221 tataccgaag aatttcaaaa attgattgct agaactggta atggacttga tcgccattta
     2281 gagacaccta caggtttatt atcaggtgga caaagacaag cgcttagttt attaatggca
     2341 actttgaaaa aaccaaatct gttattattg gacgaacaca cagcagcgct tgatcctaga
     2401 acaagtgttt cgcttatggg cttgactgat gagtttatca aacaagattc gttgactgcc
     2461 ttaatgatta cacatcatat ggaagatgct ctcaaatatg gaaatcgtgt attagtaatg
     2521 aaagatggca agattgtcag agatttaaat caggcacaaa aaaacaagat ggctatagct
     2581 gactattatc aattatttga ttaaaaagaa gtgaaatgga agtgtttcac ttctttttct
     2641 atacactttg caagaaatta ttttcaaaat atgatatact aataatgctg taaatcgact
     2701 tgtagagaca aggaccgtaa gtacaaatgg aatgaaaa
//
LOCUS       DATROQ010000153         2726 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.154, whole genome shotgun sequence.
ACCESSION   DATROQ010000153 DATROQ010000000
VERSION     DATROQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.154"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(77..1459)
                     /locus_tag="VBC94_001994"
     CDS             complement(77..1459)
                     /locus_tag="VBC94_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6980219.1"
                     /translation="MLTTKENFKGNNKLLVGIVLAVLSFWLFAQSILNMGPDVQSSLG
                     ISSGAMDIGVSSTALFSGLFIVVTGGLADKLGRVKFTFIGLCLNIIGSLLIVLANGAV
                     LFIMGRIFQGLAAAFIMPSTMALVKTYYDGKDRQRAVSFWSIGSWGGSGLCSYFGGAV
                     ASTLGWRYVFIFSIIASVVSFLLILGTPESKNVGQKTHFDYLGLIIFIISMLSLNIGI
                     SMAQEHGLMNVIPLSLFTVMLIGFVLFYYVETRKSNSFIDFHLFENRFYLGATISNFL
                     LNAVAGTLIVINTYMQQGRQLTPKVAGEMSLGYLVCVLIAIRVGEKILQRFGARKPML
                     LGAMSTFVGIFLMTLVNIQGPLYLVLVFVGYALFGTGLGIYATPSTDTAISSIPNEKV
                     GSASGIYKMASSLGGAIGVATSIAIYHAFSGNADFHKAALCGLILNLVFCSLSILSIL
                     FVIPKKIEDK"
     gene            complement(1467..2681)
                     /locus_tag="VBC94_001995"
     CDS             complement(1467..2681)
                     /locus_tag="VBC94_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO6980220.1"
                     /translation="MANASLRHQLFEKLDQKCDQMVAIRRYLHENPELSFKETKTAAY
                     ISDFYKGKDCHVQTQFGGMNGVVVDIYGDKATDKPIKHIALRADFDALPIQEETGLSF
                     ASKTAGVMHACGHDAHTAYLLILAESLIELKSEFSGHIRILHQPAEEVPPGGAKAMIE
                     AGCLDGIDAVLGIHVMSTMEEGTVQYHAGPIQTGRATFKVILQGKGGHGSMPHRANDT
                     IVAASSFVMAAQTIVSRRVNPFDTAVVTIGSFDGKGSANVIKDSVTLEGDVRVMSEET
                     RGVVEEEFKRILDGIAQTYGVSYQLDYQNDYPVLVNNSEVTQKVANSLKSVAIKEILD
                     VIDCDPQTPSEDFAYYAQTIPACFFYVGAHEEGQPYYPHHHPKFQIAESSLMVSAKSM
                     ATAALAMLVEGE"
BASE COUNT          929 a          575 c          421 g          801 t
ORIGIN      
        1 tattttaaat ttctttccca attcctttta cattattaca acaatacttg agattttgaa
       61 tctcaagtat tgttatttat ttatcctcaa ttttcttagg gataacaaaa agaattgata
      121 agatcgatag actacaaaaa actaggttta aaattaatcc acacaaagct gctttgtgaa
      181 aatctgcatt accagaaaaa gcatgataaa tcgcaattga agttgctaca ccaattgcac
      241 cacctagtga acttgccatt ttgtaaattc cagatgcaga acctactttt tcattaggaa
      301 tactagaaat agctgtatct gttgatggtg tcgcatagat ccccaagcct gttccaaata
      361 aagcataacc aacaaagact aacacaagat acaaaggtcc ttgaatattg accaaagtca
      421 ttaaaaatat cccaacaaat gtactcatag ctcccaaaag cattggctta cgggcaccaa
      481 aacgttgtaa aattttctca ccaactcgga tagcaattag tacacaaacc aaatagccta
      541 aagacatttc acctgcaacc tttggtgtta gctggcgacc ttgttgcatg taagtattaa
      601 taacaatcag agttcctgca actgcattca gtaagaaatt tgatattgtc gcacctaagt
      661 aaaatcgatt ttcaaacaga tgaaagtcaa taaaactatt agatttacgt gtttcaacat
      721 aataaaacag aacaaatcca ataagcatca ctgtaaaaag actcaatgga ataacgttca
      781 tcaatccatg ttcctgagcc atagatatcc caatatttaa cgataacatt gaaataataa
      841 aaataattaa accaaggtaa tcaaagtgag ttttttgtcc aacattttta ctttctgggg
      901 ttcctaaaat taatagaaaa ctaactactg atgcgatgat tgaaaagata aagacataac
      961 gccaacctag agtacttgcc accgctccac caaaataaga acatagacct gagccacccc
     1021 acgatccgat tgaccagaaa ctaactgcac gttgtctatc tttaccatca tagtacgttt
     1081 taaccaaggc cattgttgat ggcataataa aagctgctgc caatccttga aaaatacgtc
     1141 ccataataaa tagcactgct ccattagcta aaacaattag tagagaacca ataatattta
     1201 agcaaagacc aataaaagta aatttaactc gaccaagttt atctgctaaa ccaccagtta
     1261 caacaataaa taaacctgaa aacaatgccg tacttgagac accaatatcc attgcaccag
     1321 atgaaatacc taatgatgac tgaacatctg gtcccatgtt taaaatagac tgtgcaaata
     1381 accaaaatga tagtacagcg agtacaattc ccaccaataa tttgttattt cctttgaaat
     1441 tttcctttgt cgttaacata tgttatctat tctccttcta caagcatagc aagcgctgct
     1501 gttgccattg atttagcaga taccatcaat gatgattctg caatttggaa ttttggatga
     1561 tgatgtgggt aatatggttg cccctcttca tgtgctccaa catagaagaa gcaagctggg
     1621 attgtttgag cataataggc aaaatcttca gacggcgttt gagggtcgca atcaatgaca
     1681 tctaaaattt cttttatagc aacagatttt aaactatttg caactttctg tgtcacttca
     1741 ctgttattaa ctaaaacagg ataatcattc tgatagtcta actgataact aacaccataa
     1801 gtttgtgcta ttccatctag aatacgctta aattcctcct caacaacacc tcgtgtctct
     1861 tcagacatca cgcgcacatc accttctaag gttacactat ctttgatcac attagcagaa
     1921 cctttaccat caaaagatcc gatagtaact actgctgtgt caaatggatt aactcgacga
     1981 ctgacaatcg tttgcgctgc cataacaaaa ctagaagctg caacaattgt atcatttgca
     2041 cgatgtggca tggaaccatg tccaccctta ccttgcaaaa taactttaaa agtggcacgc
     2101 cctgtttgaa tgggacctgc gtgatattgt accgttccct cttccatcgt tgacataaca
     2161 tggataccaa ggacagcatc aatgccatct aaacatcctg cttcaatcat tgcttttgct
     2221 ccgcctggtg gcacctcttc agccggttga tgtaagatac ggatatgacc tgaaaattct
     2281 gattttaatt caatcaagct ctcagccaaa atcaataaat aagctgtatg ggcatcatgt
     2341 ccacaagcat gcatgacgcc cgctgttttt gaagcaaaag aaagccctgt ctcttcctga
     2401 attggaagag catcaaagtc ggctctcaaa gcaatgtgtt tgatgggctt atctgtcgct
     2461 ttatcaccat agatatcaac tacgacgcca ttcataccac caaattgtgt ctgaacatga
     2521 cagtccttac ctttataaaa atctgaaata taagctgctg tttttgtctc tttaaatgat
     2581 aattcgggat tttcatgtaa atagcgacga atagcaacca tttgatcaca tttttggtca
     2641 agtttttcaa ataattgatg tcgcaaagat gcattggcca tttgctttcc tccttaaaat
     2701 atgaacagtt aaaataaggc tattat
//
LOCUS       DATROQ010000154         2545 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.155, whole genome shotgun sequence.
ACCESSION   DATROQ010000154 DATROQ010000000
VERSION     DATROQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.155"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            122..967
                     /locus_tag="VBC94_001996"
     CDS             122..967
                     /locus_tag="VBC94_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HEO6980221.1"
                     /translation="MLSVGLVLEGGGMRGLYTAGVLDAFLDAGIKIDGIVSVSAGALF
                     GVNFVSRQRERALRYNKKYLSHPEYMSLRSWLRTGNFVNKDFTYYEVPMKLDVFDDEA
                     FKKSSIDFYVVATEMTSGKPEYFKIDSVFEQMEILRASSALPVVSKMVDWQGKKYLDG
                     GLSDSIPVDFARGLGFDKLIVVMTRPLNYQKKPSSGRLYKTLYRKYPNFVKTASNRYQ
                     QYNNSLEKVMSLEKTGDLFAIRPSKSLVIGRLEKNPDKLDSIYQLGMKYAKSVMPELN
                     SYLMK"
     gene            complement(1010..2257)
                     /locus_tag="VBC94_001997"
     CDS             complement(1010..2257)
                     /locus_tag="VBC94_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6980222.1"
                     /translation="MSPLNINKNNWRALIAAIVASGTDDLNIMFLAFSMSTIITDLHL
                     SAAQAGWIGTITNLGMLVGGLIFGLLADRYNKFKVFKWTILIFSIATGLVFFTTNLSY
                     LYIMRFIAGIGVGGEYGIAIAIMAGIVPANKMGRISSLNGIAGQVGSISSALLAGWLA
                     PALGWRGLFLFGLLPIVLVLWMQFAIDDKDILDQYNTDADDEPLDISIKALFDTPVLA
                     TQSLALMVMTTVQIAGYFGMMNWLPTIIQTNLNVSVKNSSLWMIATIFGMCLGMLVFG
                     QLLDKFGPRLVYGCFLLSSAICVYLFQFATTMPSMIIGGAVVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNLILNVGRAIGGFSSVIIGMILDVSNVSMVMLFLASLYIVSFLSML
                     SIKQLKRQKYHTNLTQLDVKPTD"
BASE COUNT          858 a          411 c          512 g          764 t
ORIGIN      
        1 attcagaaat tattattaaa ttaattatgg agtttatata gtacaacata ttctttttca
       61 gaaaaaacag ctatattttg ctatacttta atcaaatatc aatattagaa agtgattgcc
      121 tatgttgtct gttggtttag ttttagaggg tggcggaatg agaggtcttt atactgctgg
      181 agttttagat gcttttctag atgcaggaat aaaaatagat ggtatcgtat ctgtctctgc
      241 gggtgcattg tttggtgtta attttgtatc tagacaacga gagagggctt tgcgatacaa
      301 taaaaagtat ttatcccacc ctgaatatat gagtctaaga tcatggcttc gaacagggaa
      361 ttttgttaat aaagatttca cctattatga agttcctatg aaattggatg tatttgacga
      421 tgaagcattt aaaaaatcaa gtattgattt ttacgtagtt gctacagaga tgacatctgg
      481 taaacctgaa tattttaaaa ttgatagtgt ttttgaacaa atggaaattt tacgtgctag
      541 ttcagcatta ccagtagtct caaagatggt tgattggcag gggaaaaagt acttagatgg
      601 tggtttatct gatagtattc ccgttgattt tgcccgtggt ttaggatttg acaagttgat
      661 tgttgtgatg actaggccgc tcaattatca gaaaaagcct tcaagtggac gattgtataa
      721 aactctgtat aggaaatatc ctaattttgt aaagacagcc tcgaatcggt accaacagta
      781 taataatagt cttgaaaagg tcatgagcct tgaaaaaaca ggcgatctat ttgcaattag
      841 accgagtaag agcttggtta ttggccgctt agagaagaat ccggataaac ttgatagtat
      901 ttatcagctt ggtatgaaat atgctaaaag tgtgatgcct gagctgaata gttatctaat
      961 gaaataaaga aaacagtatt tctcaattga ggaatactgt tttgtaatat cagtcagttg
     1021 gtttgacatc taattgtgtt aaattagtgt gatatttttg acgttttaat tgcttaatgc
     1081 ttagcattga taagaaacta acgatataga gacttgctaa gaaaagcatg accatagaga
     1141 catttgaaac gtctagaatc attccgatga taacagacga aaagccacct attgcacgac
     1201 caacatttaa gataagattg ttagctgttg aacgaatatg atgtggatag agacgtgtaa
     1261 tcatggcacc atagcctgca aacataccat taacaaagaa gccgacaact gccccgccta
     1321 taatcataga aggcattgtt gttgcaaatt gaaagagata gacacaaatt gcagatgata
     1381 gaaggaaaca cccgtatact aaacgcggtc caaatttatc taacaattga ccaaatacta
     1441 gcatgccaag acacattcca aaaatggttg caatcatcca taatgatgaa ttcttgacag
     1501 aaacgtttaa gttggtttgg ataatagttg gtaaccagtt catcatgcca aagtatccgg
     1561 caatctgaac agttgtcata accattagtg ctaggctctg tgtggctaat acaggagtat
     1621 caaataaagc ttttatactg atatctagag gttcatcatc tgcgtctgta ttatattgat
     1681 ctaagatatc tttatcatca atagcaaatt gcatccatag gacgagaact ataggaagaa
     1741 ggccaaagag aaaaagtccc cgccaaccga gtgctggtgc tagccatcct gctaagagtg
     1801 ctgagctaat agatccgact tgaccagcga taccatttag tgaagaaatc cggcccattt
     1861 tattagctgg gacaattcct gccataatgg caatagcgat accatattcg ccaccaacgc
     1921 caattcctgc aatgaagcgc atgatataga gatatgataa attagtagtg aagaaaacta
     1981 aaccagttgc gattgaaaag attagaatcg tccatttgaa aactttgaat ttattgtagc
     2041 gatcagctaa taaaccaaag attagtcctc ccacgagcat tcccaagttg gtaattgtcc
     2101 caatccaacc agcctgtgcc gcagataaat gaagatcagt aatgatagtt gacatagaaa
     2161 atgcaaggaa catgatattt aaatcatcgg ttccggatgc gacaatggca gcgattaggg
     2221 cgcgccaatt attcttattg atatttaatg gtgacataag tctcctcctt taatgatggc
     2281 tcacagacca ccgttattaa atactgtcaa tagaaaaaat tctaaagcag agatgtcata
     2341 gctgcttcaa ctcattataa tgtaaaagaa aaaactcaag ctgctaccct tgagttctat
     2401 aaacgctcgc aaaaatagta ggatttctgt atagaatcct attttggtcg cttatatgtc
     2461 tcacaggaca cggttaaagc attatgtcat ttggaaatag tatagagtaa gtttagttta
     2521 ttgtcaagga taagtgggga gagaa
//
LOCUS       DATROQ010000155         2470 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.156, whole genome shotgun sequence.
ACCESSION   DATROQ010000155 DATROQ010000000
VERSION     DATROQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.156"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..193)
                     /gene="recX"
                     /locus_tag="VBC94_001998"
     CDS             complement(<1..193)
                     /gene="recX"
                     /locus_tag="VBC94_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704976.1"
                     /note="binds RecA and inhibits RecA-mediated DNA strand
                     exchange and ATP hydrolysis and coprotease activities;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEO6980223.1"
                     /translation="MKITKIEKKKRLYTLELDNTENLYITEDTIVHFMLSKGMIINAE
                     KLENIKKFAQLSYGKNLGLY"
     gene            292..1647
                     /gene="rlmD"
                     /locus_tag="VBC94_001999"
     CDS             292..1647
                     /gene="rlmD"
                     /locus_tag="VBC94_001999"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO6980224.1"
                     /translation="MNVVLKQRIPLKIKRMGINGEGIGFYKKTLIFVPGALKGEEVFC
                     QISSVRRNFAEAKLLKINKKSKNRVDPACSIYKECGGCQIMHLQYDKQLEFKTDIIRQ
                     ALMKFKPEGYENYEIRKTIGMSEPEHYRAKLQFQVRSFGGNVKAGLYAQGTHRLIDIK
                     DCLVQDRLTQEMINRVAELLGKYKLPIYNERKIAGVRTVMIRRAQASGEVQLIFITSK
                     RLDFDDVVIELVREFPELKTVAVNINASKTSDIYGQITEVIWGQESINEEVLDYGFSL
                     SPRAFYQLNPKQTQILYSEAVKALDVKEDDDLIDAYCGVGTIGLAFAGKVKSVRGMDI
                     IPEAIQDAKENALHMGFTNTHYEAGKAEDIIPRWYSEGFRANALIVDPPRTGLDDKLL
                     NTILKMPPEKMVYVSCNTSTLARDLVTLTKVYHVHYIQSVDMFPHTARTEAVVKLQRK
                     V"
     gene            1915..2166
                     /locus_tag="VBC94_002000"
     CDS             1915..2166
                     /locus_tag="VBC94_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980225.1"
                     /translation="MIINSFDNSRKATINPEDINSPIKGFPKTVITCFARETFNRILE
                     ELPHREIARTSVANLEIPIYELEFKGQKIVLIKRLVMKI"
     gene            2163..2468
                     /locus_tag="VBC94_002001"
     CDS             2163..2468
                     /locus_tag="VBC94_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6980226.1"
                     /translation="MICLHGMNWVDGQLAAYYRLIPEDDKVHLGRVIVNPDFRKKGLG
                     NQLVEYEIKFSEANYPNKPIYAQAQAYLQDFYQSFGFQPISDIYLEDNIPHLDMVLS"
BASE COUNT          843 a          324 c          481 g          822 t
ORIGIN      
        1 aataaagacc tagattttta ccgtatgata gttgggcaaa ttttttaata ttttcaagtt
       61 tctcggcatt aattatcatc cctttactta acataaaatg aacgatagta tcttctgtta
      121 tatataaatt ttcggtattg tctagctcta gagtgtaaag tctttttttc ttttcgattt
      181 tggttatttt catagttttt attatagcaa aaatgataaa atagtaaaaa tgagtctttt
      241 tttaaatgtt atggatttat agtattttct tagttaggga gtaatgaact gatgaatgtg
      301 gttttaaaac agcgaattcc tttaaaaatt aaacgaatgg gaattaatgg tgaaggaata
      361 ggtttttata aaaaaacact aatctttgtt ccaggggctc tcaagggaga agaggtgttt
      421 tgtcagattt cttctgttag acgtaatttt gcagaagcta aactgttaaa gataaataaa
      481 aagtctaaaa atagggtaga tcccgcttgt tctatttata aagaatgtgg gggatgccaa
      541 ataatgcacc ttcaatatga taagcagcta gagtttaaaa cggatattat tcgacaggct
      601 ctaatgaaat ttaaaccaga gggatatgag aattatgaaa tccgtaaaac gataggtatg
      661 tcggaacctg aacactatcg tgctaaatta caatttcaag tgcgttcgtt tggagggaat
      721 gtaaaggcag gtttatatgc acaagggaca catcgattga ttgatatcaa agattgttta
      781 gtgcaagata ggcttactca ggaaatgata aatcgagttg cagaactttt aggaaaatat
      841 aaattgccta tttataatga acgtaaaata gctggtgttc gtactgttat gattcgacga
      901 gcacaagcga gtggagaggt tcaactgatt tttatcacaa gtaaaagact tgattttgat
      961 gatgttgtta ttgaattagt tcgagagttt cctgagttaa agacagttgc tgtaaatatc
     1021 aacgctagta aaacaagtga tatctatggt caaataacag aagttatttg gggtcaagaa
     1081 tccatcaatg aagaagtatt agattatggt ttttcgttat caccacgtgc tttttatcaa
     1141 cttaatccta aacaaacaca aattttatat agtgaggcag tcaaggcctt agatgtgaag
     1201 gaagatgatg atttaattga cgcttattgt ggagtaggaa cgattggact tgcttttgct
     1261 ggaaaagtta aaagtgttcg tggaatggat attatccctg aggctattca ggatgctaaa
     1321 gagaacgcac ttcatatggg ttttactaat acacattacg aagctggaaa ggcagaggat
     1381 attatcccta ggtggtatag tgagggattt cgcgctaatg cgttaatcgt ggatcctcct
     1441 cgcacaggct tagatgataa attgctaaat actatactaa aaatgcctcc agaaaaaatg
     1501 gtttacgttt cttgtaatac atctacgctt gctagagact tagtaacatt aacaaaagta
     1561 taccatgttc actacatcca atctgttgac atgtttccac atacagcaag gaccgaggct
     1621 gtagtgaaac tacaacgtaa ggtataataa agttttctaa tctaaaaata agagtatttt
     1681 tgggtattct tatttttaga tagtaatggt gagatattaa tatagcatag tataaaacac
     1741 atggtttaag ctatttgata cggaaattta ccttagaaat ggtaaataat tccattaagt
     1801 atattcatct ttgcaaattt gaccttgcct tttaagtttc attgggttaa aatagaaaaa
     1861 atatttttaa caagatgata gaacatcgta aattaaggtt taaaggaaat gcttatgata
     1921 ataaattcat tcgataatag tagaaaagcg actattaatc ctgaagatat taattcacca
     1981 ataaaaggtt ttccaaagac tgttattacc tgttttgcgc gggagacttt caatcgcatt
     2041 ttagaagaat tgcctcatcg tgaaattgct agaactagtg tagcaaactt agaaattccc
     2101 atttatgaat tagaatttaa aggacaaaag attgtcctta tcaagaggtt ggtgatgaag
     2161 atttgatttg tttgcatggg atgaattggg tagatggtca attggcagct tattatagat
     2221 tgataccaga agatgataag gtgcatttgg gacgtgtgat tgttaatcca gactttagaa
     2281 aaaagggatt aggaaatcaa ttagtggaat atgagattaa atttagcgag gcaaattacc
     2341 ctaataaacc tatttatgct caggcccaag cttatttaca agatttttat caatctttcg
     2401 gatttcaacc tatctcagat atctatctag aagataatat tcctcatctt gatatggttt
     2461 tatcataaat
//
LOCUS       DATROQ010000156         2448 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.157, whole genome shotgun sequence.
ACCESSION   DATROQ010000156 DATROQ010000000
VERSION     DATROQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.157"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            31..2292
                     /locus_tag="VBC94_002002"
     CDS             31..2292
                     /locus_tag="VBC94_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6980227.1"
                     /translation="MLCQNCKLNESTIHLYTNVNGKQKQVDLCQNCYQIIKTDPNNPL
                     FSGLNHVSHAPGGINPFFDDFFGDLNNFRAFNGQDLPNTPPTQAGGNRGGGNGNGRNN
                     NRNQTATPPQAKGILEEFGINVTEIARHGDIDPVIGRDSEIIRVIEILNRRTKNNPVL
                     IGEPGVGKTAVVEGLAQKIVDGNVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKL
                     MEEIRQRQDVILFIDEIHEIVGAGTAGEGSMDAGNILKPALARGELQLVGATTLNEYR
                     IIEKDAALERRMQPVKVDEPSVEETITILKGIQKKYEDYHHVKYNNDAIEAAAVLSNR
                     YIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDQRLIEAENLKAQATREEDYERAA
                     YFRDQIAKYKEMQQQKVDDQDTPIITEKTIEHIIEEKTNIPVGDLKEKEQSQLINLAD
                     DLKQHVIGQDDAVIKIAKAIRRNRVGLGSPNRPIGSFLFVGPTGVGKTELSKQLAIEL
                     FGSADSMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLILLDEIE
                     KAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGSGKTEASVGFGASREGR
                     TNSVLGQLGNFFSPEFMNRFDGIIEFKALDKENLLNIVDIMLSDVNARLAINGIHLDV
                     TDKVKEKLVDLGYDPKMGARPLRRTIQEHIEDAITDYYLENPSEKELRAIMTSNGNII
                     IKSSKKTEESTKG"
BASE COUNT          839 a          443 c          469 g          697 t
ORIGIN      
        1 tagttaaatg tcataagaaa ggggtagcct atgctctgtc aaaactgcaa actaaacgaa
       61 tcaactattc atttgtatac aaatgttaat ggaaaacaaa agcaagttga tctttgtcaa
      121 aattgctacc aaattattaa aactgaccct aataatccac ttttttcagg tttaaatcat
      181 gtttcacatg cgcctggtgg catcaatcct ttctttgatg atttctttgg agatttaaat
      241 aatttcagag cctttaatgg ccaagattta ccaaacactc ctcctacaca agcaggagga
      301 aaccgaggtg gaggaaacgg taatggacga aataataatc gcaaccaaac tgcaactcct
      361 cctcaagcca aagggattct agaagaattt ggtatcaatg tcactgaaat agcccgccat
      421 ggtgatattg accctgttat tggacgtgac tcagaaatta ttcgtgttat cgaaatcctt
      481 aatcgtcgta caaaaaataa tcctgtactt attggcgaac ccggcgtagg gaaaactgcc
      541 gttgttgaag gacttgctca gaaaattgtt gatggtaatg tgcctcataa acttcaaggc
      601 aaacaagtta ttcgtttgga tgttgtgagt ctagtacaag gaacgggcat tcgtggtcaa
      661 tttgaagaac gcatgcaaaa gttgatggaa gaaattcgtc aacgtcaaga tgttattcta
      721 ttcatagatg aaattcacga aatagttgga gctggtacag ctggcgaagg tagtatggat
      781 gcaggtaata ttttaaaacc tgcccttgca cgtggagaat tacaactagt aggtgccact
      841 actctcaatg aatatcgtat tatcgaaaag gacgctgcct tagaacgccg tatgcaacct
      901 gtaaaagtcg atgaaccttc tgttgaagaa accattacta ttttgaaagg tatccaaaaa
      961 aaatacgaag attatcatca cgtaaaatat aataatgatg ccatagaagc agctgcagta
     1021 ctatctaatc gttatatcca agaccgcttt ttacctgata aagcaataga cttattagat
     1081 gaagctggtt ctaaaatgaa cctaacacta aattttgttg atccaaaaga aattgatcaa
     1141 cgtctcattg aagcagaaaa tttaaaagcg caagcgactc gtgaagaaga ttacgaacgt
     1201 gcagcttact tccgtgacca gattgcaaaa tataaagaaa tgcagcaaca aaaggtcgac
     1261 gatcaagata cacctattat taccgaaaaa acaattgagc acatcattga agaaaaaacg
     1321 aatatccctg ttggtgattt aaaagaaaaa gaacaatctc aattaattaa tctcgcagat
     1381 gacttgaaac agcatgtgat cggccaggat gacgctgtca ttaagattgc aaaagctatt
     1441 cgtcgtaatc gagttggtct tggtagccca aaccgtccta ttggttcctt tttatttgta
     1501 ggaccaaccg gtgttggtaa aactgaactt tctaaacaac tagcaattga gctctttggt
     1561 tcagctgata gtatgattcg ttttgatatg tcagagtaca tggaaaagca tgctgttgct
     1621 aaattagtcg gagcgcctcc aggatacgtg ggatacgagg aagctggaca actaactgaa
     1681 aaggttcgtc gaaatcctta ctcgctcatc cttctagatg aaattgaaaa agctcatccc
     1741 gatgtcatgc atatgttctt gcaggtcctt gatgacggtc gattaacaga tggacaagga
     1801 agaactgtta gttttaaaga taccattatc atcatgacct caaatgctgg ttctggtaaa
     1861 actgaagcaa gtgttggctt tggtgcctca cgagaaggta ggacgaattc cgtactaggt
     1921 caactaggta acttcttcag ccctgaattt atgaaccgct ttgacggtat tattgaattc
     1981 aaggctttag ataaagagaa tctcctcaat atcgttgata ttatgttatc tgacgttaat
     2041 gcacgtctcg ccatcaatgg tattcatcta gatgtcactg ataaagtgaa agaaaaattg
     2101 gttgatttag gttacgatcc caaaatggga gcacgtccat tacgtcgtac cattcaagaa
     2161 catattgaag atgctatcac agattactat cttgagaatc caagcgaaaa agaacttcgt
     2221 gctattatga ctagcaatgg aaatatcata ataaaatctt ctaaaaaaac tgaggaaagt
     2281 acaaaaggtt gagtaagaaa ctcaaccttt tttcacactc ttctcaaaaa ataatagcct
     2341 ctataaagct gtttctctcc tatctcgtct ttaaatttta atacaattat taaggaaata
     2401 ttccaaaaat tttagtataa taaataaaaa ataattaaag cttttaaa
//
LOCUS       DATROQ010000157         2400 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.158, whole genome shotgun sequence.
ACCESSION   DATROQ010000157 DATROQ010000000
VERSION     DATROQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.158"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..245
                     /locus_tag="VBC94_002003"
     CDS             <1..245
                     /locus_tag="VBC94_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEO6980228.1"
                     /translation="SFHILKQKLFNDKHKRFKKRARYFLEENLVHFVASDMHNLDVRP
                     PFLAEAYKIICRDFGKERANQLFIENAQSILKNHYI"
     gene            254..946
                     /locus_tag="VBC94_002004"
     CDS             254..946
                     /locus_tag="VBC94_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEO6980229.1"
                     /translation="MNKIANTEVEINIFNLLKKLWKKKFLITFVAIAFATAGLFYSLF
                     IVTPQYTSSTRIYVINPNTPNNSITAQDLQAGSFLANDYKEIITSTDVLEKVISSEKL
                     NYPSSQLLQKITVSILKDTRVISISVEDANPKMSQKLANSVREAAVSKIKAVTQVEDI
                     TTLEKGNLPKAPSSPNIKKNVLIGFIVGAGLSTIVLVIMGILDDRVNTEEDIEKVLGL
                     TSLGIVPDLNKL"
     gene            957..1646
                     /locus_tag="VBC94_002005"
     CDS             957..1646
                     /locus_tag="VBC94_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEO6980230.1"
                     /translation="MTRLEIVDSKLRQAKKTEEYFNAIRTNIQFSGKENKILAIASVR
                     EGEGKSTTSTSLALSLAQAGFKTLLIDADTRNSVMSGTFKATGTIKGLTNYLSGNADL
                     GDIICETNVPRLMVVPSGKVPPNPTALLQNAYFNKMIEAIKNIFDYIIIDTPPIGLVV
                     DAAIIANACDGFILVTQAGRIKRNYVEKAKEQMEQSGSKFLGIILNKVNESVATYGDY
                     GNYGKRDRKRK"
     gene            1659..>2400
                     /locus_tag="VBC94_002006"
     CDS             1659..>2400
                     /locus_tag="VBC94_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO6980231.1"
                     /translation="MKEKENIQKIIIAMIQTVVVYFSASLTLTLITPNFKSNKDLLFV
                     LLIHYIVFYLSDFYRDFWSRGYLEEFKMVLKYSFYYIFISSSLFFIFKNSFTTTRLSF
                     FTFIAMNSILLYLLNSFLKYYRKYSYAKFSRDTKVVLITNKDSLSKMTFRNKYDHNYI
                     AVCILDSSEKDCYDLKHNSLRIINKDALTSELTCLTVDQAFINIPIELFGKYQIQDII
                     NDIEAMGVIVNVNVEALSFDNIGEKRIQT"
BASE COUNT          851 a          338 c          397 g          814 t
ORIGIN      
        1 atagtttcca tattttgaaa caaaaacttt ttaatgataa gcataaacgc tttaagaaaa
       61 gagcccgtta ttttttagag gaaaatttag tgcattttgt agcgagtgat atgcataacc
      121 ttgatgttag accgccattt ttagcagaag cttataagat tatctgtaga gatttcggta
      181 aagaacgtgc taaccaactt tttattgaaa atgctcaatc tatattaaaa aaccattaca
      241 tttaggagat ttcatgaata aaatagctaa tacagaagtt gaaataaata tattcaacct
      301 tttaaaaaaa ttgtggaaaa agaaattttt aatcacattt gttgccattg cgtttgcaac
      361 agctggactt ttctatagtc tttttattgt tacgccacaa tatacttctt caacaaggat
      421 atatgtcatt aaccctaata cccctaataa tagtattact gcacaagatc tacaagcggg
      481 gagttttctt gcaaatgact ataaggagat tattacgtct actgacgttc tagaaaaagt
      541 tatttcttct gaaaaattga attatccttc gtctcagttg ctacaaaaaa taacagtttc
      601 tattttaaaa gatacacgtg ttatttcaat atcggtcgaa gatgctaatc caaaaatgtc
      661 tcaaaaatta gcaaattcag ttagagaagc agcagtttca aaaatcaagg cagttactca
      721 agtagaggat atcactactc ttgagaaggg aaatttacct aaagcaccat cttctcctaa
      781 tattaaaaag aatgtactaa tcgggtttat tgttggtgca ggattatcaa ctattgtttt
      841 agttattatg gggattttgg atgatcgtgt taatactgaa gaagatattg aaaaggtctt
      901 aggacttact tctttaggta tagtaccaga tttgaataaa ctttaaggag aatataatga
      961 ctcgtttaga aatagttgat agcaagttaa gacaagcaaa aaaaacagaa gaatacttca
     1021 atgcgatccg tacaaacata cagtttagtg gaaaggaaaa taaaattctt gcaattgcct
     1081 ctgttaggga aggggaagga aaatccacta cttcaacaag tttagcttta tctttagctc
     1141 aagcaggatt taaaacatta ttaattgatg cggatactag gaactctgtt atgtctggaa
     1201 cctttaaagc aactggaact attaaaggct tgacgaatta tttatcaggt aatgcagatc
     1261 ttggagatat tatctgtgaa accaatgttc ctagactgat ggtcgttcct tcaggaaaag
     1321 taccaccaaa tccaacagca ttacttcaga acgcttattt taataagatg attgaagcta
     1381 ttaaaaatat atttgattat attatcatcg atactccacc tattggttta gttgttgatg
     1441 ccgcaataat cgctaatgct tgcgatggtt ttattttagt aacccaagca ggcagaataa
     1501 aacgtaatta tgttgaaaaa gcaaaagaac agatggaaca aagtggttca aagttcttag
     1561 gtattattct taataaagtt aatgaatctg ttgctactta cggcgattat ggaaattacg
     1621 gaaaaaggga tagaaaaagg aagtaaggga ctctggtatt gaaagaaaaa gaaaatatac
     1681 aaaagattat tatagcgatg attcaaacag ttgtagttta tttttctgca agtttgacat
     1741 taacattaat tactcccaat tttaaaagca ataaagattt attgtttgtt ctattgatac
     1801 attatattgt tttttatctt tctgattttt acagagactt ttggagtcgt ggctatcttg
     1861 aagagtttaa aatggtattg aaatacagct tttactatat tttcatatca agttcattat
     1921 tttttatttt taaaaactct tttacaacga cacgactttc cttttttact tttattgcta
     1981 tgaattcgat tttattatat ctattgaatt catttttaaa atattatcga aaatattctt
     2041 acgctaagtt ttcacgagat accaaagttg ttttgataac gaataaggat tctttatcaa
     2101 aaatgacctt taggaataaa tacgaccata attatatcgc tgtctgtatc ttggactcct
     2161 ctgaaaagga ttgttatgat ttgaaacata actcgttaag gataataaac aaagatgctc
     2221 ttacttcaga gttaacctgc ttaactgttg atcaagcttt tattaacata cccattgaat
     2281 tatttggtaa ataccaaata caagatatta ttaatgacat tgaagcaatg ggagtgattg
     2341 tcaatgttaa tgtagaggca cttagctttg ataatatagg agaaaagcga atccaaactt
//
LOCUS       DATROQ010000158         2370 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.159, whole genome shotgun sequence.
ACCESSION   DATROQ010000158 DATROQ010000000
VERSION     DATROQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.159"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            277..774
                     /gene="ybeY"
                     /locus_tag="VBC94_002007"
     CDS             277..774
                     /gene="ybeY"
                     /locus_tag="VBC94_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEO6980232.1"
                     /translation="MYVEMIDETGQVSEDIKKQTLDLLEFAAQKTGKENKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPEVDISFDEEDLAENPELAEMLEDFDSYIGEL
                     FISIDKAKEQAEEYGHSYEREMGFLAVHGFLHINGYDHYTPEEEKEMFSLQEEILTAY
                     GLKRQ"
     gene            755..1153
                     /locus_tag="VBC94_002008"
     CDS             755..1153
                     /locus_tag="VBC94_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEO6980233.1"
                     /translation="MDLNDNNQKKWKNRTLTSSMEFAVTGIFTAFKEERNMRKHLVSA
                     ILVILAGLTFQVSMVEWLFLLLSIFLVITFEIINSAIENVVDLASNYHFSMLAKNAKD
                     MAAGAVLVVSLFAVLVGLIIFIPKILALLL"
     gene            1195..2094
                     /gene="era"
                     /locus_tag="VBC94_002009"
     CDS             1195..2094
                     /gene="era"
                     /locus_tag="VBC94_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEO6980234.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGD
                     DMIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALQGNNVPT
                     LIKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
BASE COUNT          806 a          322 c          477 g          765 t
ORIGIN      
        1 tattttgaag taaatactaa ttcataagtc agaattttat tgaaatggat tataagtgtg
       61 taatagtgat tattagcttt ctctcccttt tttaatactc ttatggtata atgatgtgaa
      121 agaatagctt gaaggaagga agagaacgca cttttatttt gagaactagt tttagataaa
      181 agtgaaaaac ttaggaaagt agatgtgtcg attatttaga attatgcatt acactttatc
      241 ttttctaaac gttttgggtc gttcttacta tataagatgt acgttgaaat gattgatgaa
      301 actggacaag tatcagaaga tatcaaaaag caaactcttg atttgttaga gtttgcagca
      361 caaaaaacag gtaaagagaa taaagaaatg gctgtaacgt ttgtcactaa tgaacgtagc
      421 catgaattga atttagagta tcgagatacc gatcgtccga ctgatgttat cagtttagaa
      481 tataagcctg aggttgatat ttcttttgat gaagaagatc tggctgaaaa tcctgaactt
      541 gctgagatgc tagaagattt tgattcttat attggtgaac tttttatttc tattgataaa
      601 gctaaagaac aagcagaaga gtacggtcac tcttatgaac gtgaaatggg ttttttagct
      661 gtacatggtt ttttgcatat taacggttat gatcattata cacccgaaga agaaaaagag
      721 atgttcagct tacaggaaga aattttaact gcttatggac ttaaacgaca ataatcaaaa
      781 aaaatggaaa aatcgcacct taacatcaag catggaattt gctgttacag ggatttttac
      841 agcttttaaa gaagaaagaa atatgcgtaa gcatttggtt tctgccattt tagtaatact
      901 tgctggtctt acttttcaag tatctatggt ggagtggtta tttcttttat tgagtatttt
      961 tttggtaatt acttttgaaa ttatcaattc agctattgaa aatgtagttg atttggcaag
     1021 taattaccat ttctcgatgc tggctaaaaa tgctaaagat atggcagcag gagcggtttt
     1081 agttgtttcc ttatttgccg tattggtggg attgattatt tttataccca agatacttgc
     1141 tttactttta tgatgagaag tattacgaat aaattgaatg gagaaagaaa agaaatgaca
     1201 tttaagtcag gatttgtagc aattttaggt cgtccaaatg ttggaaaatc aaccttccta
     1261 aaccatgtta tgggacaaaa aattgctatc atgagtgata aagctcaaac aacacgtaat
     1321 aaaattatgg ggatttatac aacagaaact gagcaaattg ttttcattga taccccagga
     1381 attcataaac caaagaccgc ccttggtgat tttatggttg aatctgctta tagtacttta
     1441 cgtgaagttg aaacagttct tttcatggtt cctgccgatg aaaagcgagg caaaggcgat
     1501 gatatgatta ttgaacgtct taaggctgcc aagattcctg ttattttagt aatcaacaaa
     1561 attgataagg ttcatccaga ccaacttttg gaacagattg atgacttccg tagtcaaatg
     1621 gattttaaag aagtagtgcc aatttctgcc cttcaaggaa ataatgtgcc aaccctaatc
     1681 aagttattga ctgataatct tgaagaaggt ttccaatatt tcccagaaga tcagattact
     1741 gatcatccgg aacgattttt agtatcagaa atggttcgtg agaaggtact tcatttaacg
     1801 caacaagagg ttcctcactc agtagctgtt gttgtcgaat ccatgaaacg tgatgaagaa
     1861 actgataaag tgcatattcg tgcaactatc atggttgaac gtgatagtca aaaaggcatt
     1921 atcattggaa aacaaggtgc tatgctcaag aaaataggaa aaatggcgcg ccgtgatatt
     1981 gaacttatgt taggtgataa agtttaccta gagacttggg taaaagtcaa gaaaaactgg
     2041 cgtgataaaa agcttgattt agctgatttt ggctataatg aaaaagagta ttaatgagtt
     2101 tattttgaga tccgcaagtt agtggggaag agttagataa gttcaccgtg agacttgatt
     2161 cgcacagaaa aagcaattca cgaggataaa accaactagt cataattttg agattatatt
     2221 cacaaattaa ttaaagggag ccaaacatgc ttgttggccc cttcttaaat aaaggagagc
     2281 acatgactca agattatatt tgctacctat aaagtagagg aggtgaggat atgcataata
     2341 tattaagatt tttaggcatt gttattattt
//
LOCUS       DATROQ010000159         2302 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.160, whole genome shotgun sequence.
ACCESSION   DATROQ010000159 DATROQ010000000
VERSION     DATROQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.160"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(248..928)
                     /locus_tag="VBC94_002010"
     CDS             complement(248..928)
                     /locus_tag="VBC94_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6980235.1"
                     /translation="MGDVVENFTEGKNPKIDTLNGKTVRIEKINPDHFEDLFQVYGEL
                     STEDSLTYMSFSKFNSKNEFDVFFQTLLKSEDPYYLAIVDNNTGKALGTFSLMRIDTK
                     NRVVEMGWVVYSSKLKQTRIATEAQYLVMKYVFEELCYRRYEWKCDSLNAPSNNSAKR
                     LGFTFEGTFRQAVVYKGRNRDTNWYSILDKEWPEKKTRFERWLDDSNFDVNEYQIRSL
                     SSIEQKTK"
     gene            complement(1003..1412)
                     /locus_tag="VBC94_002011"
                     /pseudo
     CDS             complement(1003..1412)
                     /locus_tag="VBC94_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1654..2214)
                     /locus_tag="VBC94_002012"
     CDS             complement(1654..2214)
                     /locus_tag="VBC94_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6980236.1"
                     /translation="MNKFFLESERLIIRPLEESDYDSWYTSFMNRGPSLTPFDDGILD
                     MSMCDINWFKNLVNLQRKEWESDIVYIFGVFLKSGEHVGMLNIVTLARADMQWGELGY
                     VFHNQFWSNGYAFESILALLNSTYEKLGFHHIEAQITPGNERSEKLVRRLGLTYETTR
                     KDFSFENGKWTDKLIYSINLHNNNLE"
BASE COUNT          765 a          427 c          243 g          867 t
ORIGIN      
        1 aaaaaatttg tttggtaatt ttacgtaact tttacagcta ataactcata gaaaaaaaga
       61 caagataata atcttgactt ttttctttga tgcccgcgac caaagtcgag ctagcacgtc
      121 cgtgaggaca cccgattttg agtcctacca tgcaccttta aaagttaata aacatttatt
      181 taacaaagtt taagatgtta gctaaaaaaa catgtaattt tgaaatttta gtgattaatg
      241 aatgactcta ttttgtcttc tgctcaatgc ttgatagtga tctgatttga tactcattta
      301 catcaaagtt tgaatcatct agccatcttt caaaccgtgt ttttttctct ggccattctt
      361 tatctaaaat agaataccaa tttgtatctc tgttacgacc cttatataca acagcttgtc
      421 taaaagttcc ttcaaaagta aaaccaagtc gttttgcaga attattagat ggtgcattca
      481 aactatcaca tttccactca taacggcggt aacagagttc ttcaaaaaca tatttcataa
      541 ccaaatattg agcttctgtt gctatccttg tttgttttag ttttgatgaa taaaccaccc
      601 atcccatttc aacaactcta ttttttgtat caatacgcat taatgaaaaa gtccctaatg
      661 ctttaccagt attattatca acaatagcta aataataagg atcttctgat tttaatagag
      721 tttggaaaaa gacatcaaat tcattcttac tattaaattt gctaaaagac atataagtta
      781 aactatcttc agtagacaat tctccataaa cttgaaataa gtcttcaaaa tgatctggat
      841 ttattttttc tattctaact gttttcccat taagtgtatc aatcttcgga tttttacctt
      901 ccgtaaaatt ttcaacgaca tcacccacta gttgattaaa cctattgtat ctcatttaat
      961 ttttctgctt tcacaaattt ttattgtcgt ctatttaaaa tattattcct ttaaaataga
     1021 ataatatgct ctggttttcc actcaccttt tggatttttt tacaatatga cgacgttcac
     1081 cttctttttg cattcttagg cattccataa cattttcaga ttttttatta ttagttcaaa
     1141 tcgagcataa attctttcca aatctagttg ttcaaatcca attgacatca attttgaaca
     1201 agcttttatc atataacctt ttccactata atttttattt aatatataac cgaattctgc
     1261 tggaaactcg tctctaattt ctaattctat aataccaatc attttatttg attctgtttc
     1321 aatcatcgca tagattccta ataatgattt catacagaca ttcgccaaaa tcaatgtgct
     1381 tcttctgtgg tattggcttt atatcgttgc atatgaaata cagtttcaaa atcagatgaa
     1441 aattctaaaa actcatttac attagataaa tcaacaagct gaagtcgcta cctttccatt
     1501 agtactatga ttgcacattg ctaaattaca atatagtgta tccaaattat tcatttctaa
     1561 tcaatattcc ttccttaatt atctataaac cacttcaata atctccaaaa atacaatatt
     1621 ataagcttta aatttctttg taagtctata aaattattct aaattattat tatgtaaatt
     1681 tatactataa atcagtttat ctgtccattt tccattctca aatgaaaaat cttttcgagt
     1741 tgtctcatag gttaatccca atcttctaac caacttttcc gatcgttcat tcccaggagt
     1801 aatctgtgct tcaatatgat gaaaacccaa tttttcataa gtactattaa gtaatgccaa
     1861 gattgactca aatgcatagc cattactcca aaattgatta tgaaaaacat atcccaattc
     1921 tccccattgc atatctgctc ttgcaagggt aacaatattt aacattccta cgtgctcacc
     1981 gctttttaga aaaacaccaa atatatatac tatgtcactt tcccattctt tcctttgtaa
     2041 atttactaaa tttttaaacc aattaatgtc acacatagac atatcaagta tcccatcatc
     2101 aaatggagtt aaagatggtc cacgattcat aaaactagta taccaactat cataatcact
     2161 ttcttctagt ggccttataa taagtctctc tgattctaaa aaaaatttat tcaattttta
     2221 tttcctttct tccagagtaa ataaataagt gtgttttaat taaaatttcc ataatattac
     2281 ctccttgaaa agttattaac ta
//
LOCUS       DATROQ010000160         2281 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.161, whole genome shotgun sequence.
ACCESSION   DATROQ010000160 DATROQ010000000
VERSION     DATROQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.161"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..147
                     /locus_tag="VBC94_002013"
     CDS             <1..147
                     /locus_tag="VBC94_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO6980237.1"
                     /translation="KGYISSDTVMVVRKNGKIFDYVLPGEPVRLWEVATEEKVEEVLM
                     ELDK"
     gene            360..1052
                     /locus_tag="VBC94_002014"
     CDS             360..1052
                     /locus_tag="VBC94_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO6980238.1"
                     /translation="MSKVRLYIARHGKTMFNTIGRAQGWSDTPLTTFGELGIKELGWG
                     LKASNISFKEAFSSDSGRTLQTMEIILREVQQENIPYTRDKRIREWCFGSLDGGYDGD
                     LFNGVLPRVSNGDMSHLTHEEIANLICQVDTAGWAEPWAILSNRILSGFTAIAKKIED
                     IGGGNAIVVSHGMTIATFLWLIDHSTPRSLGLDNGSVSVVDFEDGTFSIQSIGDMSYR
                     EKGREILEKTLQ"
     gene            1049..1801
                     /locus_tag="VBC94_002015"
     CDS             1049..1801
                     /locus_tag="VBC94_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEO6980239.1"
                     /translation="MKKNKIIRFSLVGVLLAILCFSLFALLKPNSQQSSSQKLRNEDI
                     KKTSSQKRNKKLRLPAVSSKDWNLILVNRDHKHEELSPDVVPVENIYLDKRITKQATQ
                     FLEAARAIDSREHLISGYRSVAYQEKLFNSYVTQEMTSNPNLTRGQAEKLVKTYSQPA
                     GASEHQTGLAMDMSTVDSLNESDPRVVSQLKKIAPQYGFVLRFPDGKTAETGVGYEDW
                     HYRYVGVESAKYMVKHHLTLEEYITLLKENNQ"
     gene            1798..>2281
                     /locus_tag="VBC94_002016"
     CDS             1798..>2281
                     /locus_tag="VBC94_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEO6980240.1"
                     /translation="MRKRFSLLNFIVVTFIFFFFILFPLLNHKGKVDANSRQSVTYTK
                     EEFIQKIVPDAQDLGKSYGIRPSFIIAQAALDSDFGEKILANKYHNLFGLLAEPGTPS
                     ITLNDSSTGKKQEKQFTHYKSWKYSMYDYLAHIKSGATGKKDSYTIMVSVKNPKTLVQ
                     K"
BASE COUNT          751 a          327 c          495 g          708 t
ORIGIN      
        1 aaaggatata tcagcagtga tacagtgatg gttgtgcgta agaacggaaa gatatttgat
       61 tatgtgttac ctggtgagcc tgtaagattg tgggaagttg cgacagagga aaaagtggaa
      121 gaagtgttga tggaattaga taaataaaaa aagacttttc caagtccaaa aatcagatgc
      181 tccccagcgc aaaaattact acgttattta ctacgttgtt tttaaaatca gtgtaattga
      241 ccagataatg taaggttgta aaatgttgat ttatcaagtg attgggcaat gatagcaaat
      301 gataccatat taaggttctt ttttgttata atataataaa atgcttgtga ggtgtcagta
      361 tgagtaaagt tcgtttatat atcgctcgtc atggtaaaac aatgtttaat acaattggtc
      421 gtgctcaggg gtggagtgat actcctttaa ctacttttgg agaactcggg attaaagagc
      481 ttggttgggg actaaaggct tcaaatatta gttttaaaga ggctttttct agtgatagtg
      541 gacgtacttt acaaactatg gaaattattc tcagggaagt gcaacaagaa aatattccct
      601 atacgagaga taaacgaatc cgcgagtggt gctttggtag tttggatgga ggatatgatg
      661 gcgacctttt taatggcgtt ctgcctcgag tttcaaacgg agatatgagc catttgacac
      721 acgaagaaat tgctaatttg atttgtcaag ttgatacggc tggctgggca gaaccgtggg
      781 caatattgag taaccgtatt ctgtcaggct tcactgctat agctaaaaaa atagaggata
      841 ttgggggagg taatgcgatt gttgtcagtc atgggatgac tattgcgact tttttatggt
      901 taatcgacca tagtacccct cgtagtttag gcttagataa tggtagtgtc tcagtcgttg
      961 attttgaaga tggaacattt tctattcaat ctattgggga tatgtcctat cgagaaaaag
     1021 gaagagaaat tttagaaaaa acattgcaat gaaaaaaaat aaaattatcc gattcagttt
     1081 agttggtgtt ctacttgcga tactatgctt tagtcttttt gctttattga agcctaacag
     1141 tcaacaatca tcatctcaaa agttgaggaa tgaggatata aaaaagacat cctctcaaaa
     1201 aagaaataag aaattacgat taccagctgt atcatcaaaa gattggaact tgattttggt
     1261 caatcgtgac cataaacatg aagaattaag tccagatgtg gtgcctgttg aaaatattta
     1321 tttggataaa cgtattacga agcaagctac tcagttttta gaggctgcta gagcaattga
     1381 ttcacgagaa catttaattt cgggttatcg tagtgttgcc tatcaggaga agttgttcaa
     1441 ttcttatgtt actcaagaga tgactagtaa ccctaatttg acgaggggac aagcagaaaa
     1501 gttggtaaaa acttactctc agcctgcagg tgctagtgaa caccagactg gattagcgat
     1561 ggatatgagt actgtagatt ctttgaatga gagcgatcct agagtagtca gtcagttgaa
     1621 aaagatagct ccacaatatg gttttgtctt acggtttccg gatggtaaaa cagcagaaac
     1681 aggggtaggt tatgaagatt ggcattaccg ctatgttggg gtagagtctg caaaatatat
     1741 ggtcaaacat catttaacat tagaagaata cataacttta ttaaaggaga ataaccaatg
     1801 aggaaacgtt tttccttgct aaattttatt gttgttactt ttattttctt tttctttatt
     1861 ctttttccgc ttttaaacca taagggaaaa gtagatgcta attctaggca gagtgttacc
     1921 tacaccaaag aagaatttat acaaaaaatt gtgccagatg cgcaagatct aggaaagtcg
     1981 tacggtattc gtccttcatt tattattgca caggcggctt tggattctga tttcggagag
     2041 aaaatattag ctaataagta tcataatctg tttggtttgc ttgcagaacc aggaacgccc
     2101 tcaattacct taaatgatag tagtacagga aaaaaacagg aaaagcaatt tactcattat
     2161 aaatcttgga agtattcaat gtatgattac cttgctcata taaaatctgg agcgacaggc
     2221 aaaaaagatt catatactat aatggtgtct gttaaaaatc caaaaacttt agtgcaaaaa
     2281 t
//
LOCUS       DATROQ010000161         2243 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.162, whole genome shotgun sequence.
ACCESSION   DATROQ010000161 DATROQ010000000
VERSION     DATROQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.162"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(64..303)
                     /locus_tag="VBC94_002017"
     CDS             complement(64..303)
                     /locus_tag="VBC94_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085641.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6980241.1"
                     /translation="MSRDEVFEKMLELLRQQLGDPQLDITPESSLHDDLAIDSIALTE
                     FIINLEDVFHLEIPDEAVEHMSSVQQLLDYIIEHK"
     gene            complement(314..1306)
                     /gene="plsX"
                     /locus_tag="VBC94_002018"
     CDS             complement(314..1306)
                     /gene="plsX"
                     /locus_tag="VBC94_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEO6980242.1"
                     /translation="MKKIAVDAMGGDYAPKAIVEGVNQAISDFSDIEVQLYGDQKKIE
                     KYLTVTERVSIIHTEEKINSDDEPAKAVRRKKQSSMVLGAKAVKDGVAQAFISAGNTG
                     ALLAAGLFVVGRIKGVDRPGLMSTMPTLDGVGFDMLDLGANAENTASHLHQYAILGSF
                     YAKNVRGIEVPRVGLLNNGTEETKGDSLHKEAYELLAAEPSINFIGNIEARDLMSSVA
                     DIVVTDGFTGNAVLKTMEGTAMNIMGSLKSSIKSGGVKAKLGALLLKDSLYQLKDSMD
                     YSSAGGAVLFGLKAPIVKCHGSSDSKAVYSTLKQVRTMLETQVVDQLVDAFTDE"
     gene            complement(1384..2235)
                     /locus_tag="VBC94_002019"
     CDS             complement(1384..2235)
                     /locus_tag="VBC94_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6980243.1"
                     /translation="MKKTLYITNKLFFANWFVPVIGAVLIQVIGIIVSRRLVVKLLPD
                     DYKFNLYIALLLFIPILASILLFLIYFQKRDFTSLGFTKDNNVLTTISGFITGSLLMF
                     FIYICHWLSGFVEISSSNSFSFGPFFFLLLAYFIQSLTEEILFRGYVMTTVTKFKGSF
                     AGVLCNSMLFSFIHFRNYGITAIALFNLFLLGIIFSILFNMTKNILFVTGVHTAWNFT
                     MGCVLGNKVSGGYSPVSLFRITENSSFAIWSGGDFGFEGGVLCSLILVGCLAYFSLER
                     VKVSVRY"
BASE COUNT          813 a          447 c          305 g          678 t
ORIGIN      
        1 aacaaaaact ttatttaaaa tcaaaaaaaa gaagaaacgt tcgtattttc ttccttttta
       61 ttattactta tgttcaatga tataatctaa tagttgttgg acgcttgaca tgtgttcgac
      121 agcctcatct ggaatctcta gatgaaacac atcttccaaa ttgataataa actctgttaa
      181 agcgatagag tcaattgcta aatcatcatg taacgatgac tctggtgtga tatctaactg
      241 tgggtcacca agttgctgcc ttaataattc cagcatcttt tcaaaaactt catctcttga
      301 catgagttca ctcctattca tctgtaaagg catcaactaa ttgatcaact acttgagttt
      361 ccaacattgt acgaacttgc ttcaaagtag aataaacagc tttagaatca cttgatccat
      421 ggcattttac tattggagct tttaaaccaa aaagaaccgc acctcccgcg cttgaatagt
      481 ccatactatc ttttagctga tacaggctat cttttaatag aagtgctcct aatttagctt
      541 tcacgccacc tgatttaatt gaagatttta gacttcccat aatattcata gctgtacctt
      601 ccattgtctt tagaacagca tttccagtaa aaccatcagt gacaacaata tctgctacac
      661 tagacatcaa atcacgtgct tcaatatttc caataaaatt aatagaaggc tctgctgcta
      721 acagttcata ggcttcttta tgaagagaat cgccttttgt ttcttccgtt ccgttattta
      781 agagacctac acgaggaact tcaataccac ggacattttt tgcataaaac gaccctaaaa
      841 tagcatattg atgaagatga gaagctgtgt tttctgcatt agctcctaaa tcaagcatat
      901 caaaacccac accatctaac gtaggcatag ttgacattaa gcctggtcga tcaacacctt
      961 ttatacgtcc tactacaaac aaaccagcag ctaataaagc tcctgtattc cctgcagaaa
     1021 taaaggcctg tgcaacacca tctttgactg ccttagctcc taagaccatc gaagactgtt
     1081 ttttacgacg aacagcttta gcaggctcat catcagaatt aattttttct tcagtatgaa
     1141 taatactaac tcgctccgtt actgttaaat atttctcaat ttttttctga tcgccataga
     1201 gttgtacttc aatatcacta aaatctgaaa tagcttgatt aacaccctct acaatagctt
     1261 tgggagcgta atctccaccc atagcatcta cagcaatttt cttcatgaat gaactcctca
     1321 taaaaactta gtggctatat tatagcatgt ttctttattt ttttcacaag aaggcaataa
     1381 tagctaatat ctaacagata ctttcactct ttccaaagaa aagtatgcta aacaacctac
     1441 taatatcaaa gaacatagta ctcccccttc aaaaccaaaa tccccaccac tccaaatagc
     1501 aaagctagaa ttttcagtaa ttctaaaaag agaaacagga gaataccccc cactaacctt
     1561 attacctaat acacatccca tagtaaaatt ccatgcagta tgaactcctg taacaaaaag
     1621 aatatttttg gtcatattaa aaagaataga aaaaataata ccaagtaaaa ataaattaaa
     1681 aagtgctatt gctgtgatac catagtttct aaagtggata aaggaaaaca acatagaatt
     1741 gcataaaaca cctgcaaaac tacctttaaa tttggtaact gttgtcataa catatcctct
     1801 aaataaaatt tcctctgtca agctttggat aaagtatgca agtaataaaa agaaaaaagg
     1861 tccaaaagaa aaggaattgc tggaagaaat ttccacaaaa cctgataacc agtggcaaat
     1921 ataaataaaa aacatcagta aactaccagt aataaatcct gagatcgttg ttaaaacatt
     1981 attatcttta gtaaatccca aggaagtaaa atcccttttt tgaaaataga ttaaaaatag
     2041 taaaatactt gccaaaatag gaataaatag taaaagcgca atgtataaat tgaatttata
     2101 atcatcaggt agcaatttta ctacaaggcg tctacttaca atgatgccga taacttgaat
     2161 tagaactgca ccaataactg gcacaaacca gttagcaaaa aagagtttat tggtaatgta
     2221 taatgttttt ttcatctcaa ata
//
LOCUS       DATROQ010000162         2229 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.163, whole genome shotgun sequence.
ACCESSION   DATROQ010000162 DATROQ010000000
VERSION     DATROQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.163"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            1..858
                     /locus_tag="VBC94_002020"
                     /pseudo
     CDS             1..858
                     /locus_tag="VBC94_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
     gene            889..1068
                     /locus_tag="VBC94_002021"
                     /pseudo
     CDS             889..1068
                     /locus_tag="VBC94_002021"
                     /inference="COORDINATES: protein motif:HMM:NF018196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
     gene            1075..1410
                     /locus_tag="VBC94_002022"
                     /pseudo
     CDS             1075..1410
                     /locus_tag="VBC94_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            1616..2155
                     /gene="nusG"
                     /locus_tag="VBC94_002023"
     CDS             1616..2155
                     /gene="nusG"
                     /locus_tag="VBC94_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEO6980244.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGKTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRSILISMGQTVDVFDTNIKEGDVVQIIDGAFIGQEGRVVEIENNKVKLMI
                     NMFGSETQAELELYQVAEL"
BASE COUNT          835 a          353 c          437 g          604 t
ORIGIN      
        1 aatacatcta atgatgtgaa cgacgttaaa cgtctattaa atgaatttta taacgcagtt
       61 gcaaggaaac agttagatac aaattctgct gactaccgta gtaaaattga taatatcagt
      121 actacaggtc ttgcgatagc tcttgaggct aaagaaattt atgaagcaaa taaatctata
      181 ttacctcatc gttacaaaga ttctgttgga acttatgtga acagttttga ggaaagacga
      241 agtccaggaa aatttaatat ttggaatggt caggaaggat ttaatgcagc tcaaaaattg
      301 ttagaagatg ttaaaaaatt attacttgag ctacaaaatt taacaaaaaa taacaaacca
      361 aatattcaag tacctaaaca agcacctaca gaagctgcaa aaccagcttt gtcaccagaa
      421 gccttgacaa gattgactac atggtataat caagctaaag atctgcttaa agatgatcaa
      481 gtaaaggaca aatacgtaga tatactttca gttcaaaaag ctgttgacca agcttatgat
      541 catgtggaag agggaaaatt tattaccact gatcaagcaa atcaattagc taacaagcta
      601 cgtgatgctt tacaaagttt agaattaaaa gataaaaaag tagccaaacc agtagctaaa
      661 ggtacatacg atgttaagta tgtagacaca gaaggaaaag aagtagctaa gtcacgtcac
      721 ttcgaaggag aagaaggcgc agcttttgtc acttcagcga aagaagtagc gggttacaaa
      781 cttgttagaa cggaaggcgc tgtttcaaat gtcttcacag caggagcaca agtacgtaca
      841 tatgtttacg aaaaagtagc caaaccagta gctaaaggta catacgatgt taagtatgta
      901 gacacagaag gaaaagaagt agctaagtcg cgtcacttcg aaggagaaga aggcgcagct
      961 tttgtcactt cagcgaaaga agtagcgggt tacaaacttg ttagaacgga aggtgctgtt
     1021 tcaaatgtct tcacagcagg agcacaagta cgtacatatg tttacgaaaa agttaaacca
     1081 gaagttaaac cagacgttaa gccagaggcc aaaccagagg ctaagccaga agttaaacca
     1141 gacgttaagc cagaggccaa accagaggct aagccagaag ttaaatcaga cgttaagcca
     1201 gaggctaagc cagaagccaa gccagcaacc aaaaaatcgg ttaatactag cggaaacttg
     1261 gtggctaaaa aagctattga aaacaaaaag tatagtaaaa aattaccatc aacgggtgaa
     1321 gccgcaagtc cactcttagc aattgtatca ctaattgtta tgttaagtgc aggtcttatt
     1381 acgatagttt taaagcataa aaaaaattaa atttattttt aaacataaat cgaaaggtct
     1441 atcctttcaa aatcttaagg caaacctcat cagaactttg ccttttttat ttgaatttta
     1501 gaatgattta gtcggtactt taattgacag cttattttat tttctttata atatatattg
     1561 aaaatagcag tccaataagc cgtaaaaggc ttttttatta aggaaaggaa aacttatgtt
     1621 agattctttt gataagggtt ggtttgtact ccaaacctac tcaggttatg aaaataaagt
     1681 aaaggaaaat ctcttgcaac gtgcgcaaac ttataatatg ttagacaaca ttttgcgtgt
     1741 ggaaatccct actcaaactg taaatgttga gaaaaatggg aaaacaaaag aaattgaaga
     1801 aaatcgcttc ccaggttatg tacttgtgga aatggtaatg actgatgaag cctggtttgt
     1861 tgttcgtaat actccaaacg ttactggatt tgttggatca cacggcaatc gctctaaacc
     1921 aacaccgttg ttagaagaag agattcgttc aatactcatc tcaatgggac aaaccgttga
     1981 tgtctttgat actaatatca aggaaggtga tgttgttcaa atcattgatg gtgctttcat
     2041 cggacaagaa ggacgtgtag ttgagattga gaataacaaa gttaaattaa tgatcaacat
     2101 gttcggctca gaaacacaag cagaacttga gttgtaccaa gttgcagaat tataaaactt
     2161 ctgaaaaatc catcttaaaa taaagatgga tttttgttaa tttataattg atttaggttt
     2221 aattcgtta
//
LOCUS       DATROQ010000163         2226 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.164, whole genome shotgun sequence.
ACCESSION   DATROQ010000163 DATROQ010000000
VERSION     DATROQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.164"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..1148
                     /gene="secY2"
                     /locus_tag="VBC94_002024"
     CDS             <1..1148
                     /gene="secY2"
                     /locus_tag="VBC94_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEO6980245.1"
                     /translation="STVPLSHQEIKLAVDQHLLNNFSAVSGGSFNKLNVFTLGLSPWM
                     SSMIIWRFVSLFSWAKNATKRKAEVAQYTLMLTISVIQAYGVSGNQFIKSSLLGSYSD
                     IVFKALTMVLLISGSFVLMWLANLNASKGLGGSIVIILVSMLQATTTGIITYFEQSHF
                     SIMDLLGTIIAISIILSVLLLISTVVYRAEYRIPIRRIDIVSKFAQDTYIPIRINPAG
                     SYPFMYGMTLMMLPTFIIQALLLAFPNERLLIQISSMIAINKPLGIGLFVILLFILSI
                     GFAYFNYDPYNIAKNMRQRGEYFQNVTPGAATQRFLQEKISIMAVVGAIFTVIMGGLP
                     MLAVVGKTNQVSIAMIVINIYIISTFMLSIIEQVNTLKLWGSHRNII"
     gene            1158..>2226
                     /gene="asp1"
                     /locus_tag="VBC94_002025"
     CDS             1158..>2226
                     /gene="asp1"
                     /locus_tag="VBC94_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEO6980246.1"
                     /translation="MFYFIPSWYNSDQKWHNEAPIWYRVLENMTFDDTVSQVRMFQNA
                     DEEVRLLLFSYNPQLRYMLHEESMLAVSYWSFFDDIQNIQQKEIKLINFKELNWPKGT
                     YFVYTPFLVLAKHGGEIIARIQFAENGNLLLIELLIDGVLDKIMIFDDRGFVSSIKYF
                     KQNRLIYQDYLNQNGVWQIREYASKFPRIVVNPMSDKVFNFTEYDDWDTLIRERILVL
                     KKLEVTPDDYIIVAVNDIHNNLVRKSFPDQKKIFSFFGERYSFDNRLDFNELLDEGEL
                     FIAESEKIERLLITSLQEKNYSSNYVENHISRIPPFDTRLRLGQSQNIEELIAYLLVD
                     DLSQTYLSEVLDQLLAKMEVNP"
BASE COUNT          705 a          309 c          416 g          796 t
ORIGIN      
        1 tatccactgt tcctttatca catcaagaga taaaactagc agtagatcaa catttactca
       61 ataacttttc agcagtaagt ggtgggagtt ttaataaatt aaatgttttc acactggggt
      121 tgagtccatg gatgtcaagt atgattattt ggagattcgt ttccttattt tcgtgggcaa
      181 aaaatgcaac gaagcgaaaa gcagaagtag ctcaatatac tttaatgctt actatctcag
      241 ttatacaagc atatggtgtt tcaggaaatc aatttataaa aagctcttta ttaggttctt
      301 atagtgatat tgtttttaaa gcattgacaa tggtcttgtt aataagtggc tcatttgtgt
      361 taatgtggct tgctaatctc aatgcttcta aagggctagg aggaagtatt gttatcatat
      421 tagtgagcat gctccaagca acgactactg gaataataac ttattttgaa cagtcacatt
      481 ttagtattat ggatctgctg ggaactatta tagctataag tataatactt agtgttttat
      541 tattaatctc aacggtggtt tatagggcag aatatcgtat acctattagg cgtattgata
      601 tcgttagtaa atttgcccaa gatacctata ttcctattcg cataaatcca gcagggtcct
      661 accctttcat gtatgggatg acattaatga tgcttccaac gtttataatt caagcattgt
      721 tacttgcttt tccaaacgag cgattattga ttcagatctc gtcaatgatt gcaattaata
      781 agcctttagg tattggactt tttgttatat tattatttat tttgagcatt ggatttgcat
      841 attttaatta cgacccgtat aatattgcaa aaaatatgcg tcaacggggg gaatattttc
      901 aaaatgtaac tccaggagct gctactcaac gttttttaca agaaaaaatt agtatcatgg
      961 cagtagttgg agcaattttt acggttatta tgggaggtct cccaatgttg gctgtggtgg
     1021 ggaagacaaa ccaagttagt attgcaatga tcgtcattaa tatttatatt atttcaactt
     1081 ttatgctcag tattattgag caagttaata ccttgaagtt gtggggcagt caccgaaata
     1141 ttatttaagg agcaatcatg ttttatttta ttccttcgtg gtacaattct gatcaaaagt
     1201 ggcataatga agctcctatc tggtatcgtg tattggagaa catgactttt gatgatactg
     1261 ttagtcaggt tagaatgttt cagaatgcag atgaggaagt gcgtttactt ctatttagtt
     1321 ataatcctca gttgagatac atgcttcatg aagagagtat gttggctgtc tcttattggt
     1381 ctttttttga cgatattcaa aatattcaac aaaaagagat taagttaatt aattttaaag
     1441 aactgaattg gcctaaaggg acttattttg tttatacacc ttttttagta ttagctaaac
     1501 atggtggcga gattattgca cgtatccaat ttgcggaaaa tgggaattta ctactgattg
     1561 aacttttaat agatggtgtg cttgataaga ttatgatatt tgacgatcgt ggttttgtat
     1621 ccagtatcaa atatttcaaa caaaatagat taatttatca agattattta aatcaaaatg
     1681 gtgtttggca aattcgagag tatgcttcaa aatttccgag aatagtagtt aatccaatgt
     1741 ctgataaggt gtttaatttt actgagtatg atgattggga tactcttatt cgagagagaa
     1801 tattggtttt aaaaaaacta gaagtgactc cagatgatta tattatagta gcagtaaacg
     1861 atattcataa caatttagtc agaaaatcat ttccagatca aaagaaaatt ttctcatttt
     1921 ttggtgaacg ctattcattt gataatcgac tcgactttaa tgaacttctt gatgaaggtg
     1981 aattgtttat tgctgaaagc gaaaaaatag aacggttatt gattacttca ttacaagaga
     2041 aaaattattc gtcaaactat gttgagaatc acattagccg tattccccct tttgatactc
     2101 gtttgagact cggacagagc caaaatattg aagagcttat tgcatattta cttgtagatg
     2161 atctttctca aacttattta agtgaagttt tagaccaatt attagcaaaa atggaagtta
     2221 atccgt
//
LOCUS       DATROQ010000164         2202 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.165, whole genome shotgun sequence.
ACCESSION   DATROQ010000164 DATROQ010000000
VERSION     DATROQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.165"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            16..723
                     /locus_tag="VBC94_002026"
     CDS             16..723
                     /locus_tag="VBC94_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEO6980247.1"
                     /translation="MKIDKKELLALIASIILLIFASVTFFLFKDHGTTQMDTVESSVN
                     HVSDSQLTEAQDMLDKFEKKPSEKLLKDVELALNKLSNSSKKEALQKRFKKAKDKYLK
                     DEADKKATKDATDLVEILEQAPSEENVLKAEAAVKKLTVKESKEALQKRIDTVKTQYG
                     LIGNQTPSSSVAETTEQGTANPASQDTSSYVNQDVAPTYEQPQTNNTPVTPGVNNTVP
                     TPGTGTVPATNGTGVAQ"
     gene            1001..2149
                     /gene="nagA"
                     /locus_tag="VBC94_002027"
     CDS             1001..2149
                     /gene="nagA"
                     /locus_tag="VBC94_002027"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEO6980248.1"
                     /translation="MTKYIKADRFFYADHVKENGYLEIKDNHFGKWIENISGQEEILD
                     YSGYQIAPGLVDTHIHGFAGADVMDCDSEGILRMSAGLLSTGVTSFLPTTLTSDTKRL
                     EEASKAVAAVAGKEQGAKIQGIYFEGPYFTEEYKGAQNPIYMRNPNLEEFAQWQKAAK
                     GLITKIALAPEREGVEEFVSAITKQGVTVALGHSNGTYKEAKKAVKAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVDPVACDILMTQKGHNHVALITDCMAA
                     GGAPDGDYMLGELPVVVSNGTARLKDGGNLAGSILKLKDGLKNVVEWGIATPEEAVRM
                     ASYVPAVSVGIDNVCGQIKEGYAADFIVLDKHLDLVATYLDGEKRWQS"
BASE COUNT          751 a          328 c          477 g          646 t
ORIGIN      
        1 actttaggag tgagtatgaa aatagataaa aaagaacttt tagcacttat tgctagtatt
       61 attttattaa tttttgcttc tgttacgttt ttcttattta aagatcatgg cactactcaa
      121 atggatactg tagaaagcag tgttaaccat gtcagtgatt ctcaattaac tgaggcacaa
      181 gatatgttag ataaatttga gaagaaacct tcagaaaaat tattgaagga tgtcgaactt
      241 gctttaaata aattgtccaa ttcttctaaa aaagaagctt tgcaaaaacg ttttaagaaa
      301 gcaaaagata agtatcttaa agacgaagca gataagaaag ctaccaaaga tgctacagat
      361 ttagtagaaa ttctagaaca agccccttca gaagaaaatg tgttaaaagc tgaagcagca
      421 gtcaaaaagt taactgtcaa agaatctaaa gaagctttac aaaaacgtat tgatactgtt
      481 aagacacaat atggtttgat tgggaatcaa actccctcta gttcagtagc tgaaactaca
      541 gaacaaggga cagctaatcc tgctagtcag gatacttcta gttacgttaa tcaggatgta
      601 gcaccaactt atgagcaacc gcaaacgaat aatacaccag ttactccagg ggttaacaat
      661 actgttccga ctccaggaac tggtactgta cctgctacta atgggacggg tgttgctcag
      721 taaaatcctg aaagtctagt tttactagac ttttttgcta tgctttttaa ttagccattc
      781 tcatcaaaaa ctataagaga attagcaatt gatagttttg gagaaggaaa agaattatga
      841 gcaatagcaa ttaaaacctt aaaagtacaa ccaagtgaag tggtttataa caagtgtata
      901 gttctttctt agaataaacc atgacttttt aaaaaatagg tatataccat ttacaaaaat
      961 accttaaagc gttataatga gagtgaatgg ggggaagaag atgacgaagt acatcaaggc
     1021 tgaccgattc ttttatgctg atcatgtaaa ggaaaacggt tatttagaaa taaaagataa
     1081 tcactttggt aaatggatag aaaatatttc tggacaagag gagattttag attattcagg
     1141 ttaccaaata gcacctggtt tagttgatac acatattcat ggttttgctg gcgctgatgt
     1201 tatggattgt gattcagaag gaattttgcg aatgagtgct ggcttactat ctactggagt
     1261 cacatctttc ttgccgacaa ccctaacttc agatacaaag cgactagaag aggcgtctaa
     1321 agcagttgcg gcagtagcgg gcaaagaaca aggagcaaaa attcaaggca tctattttga
     1381 gggaccatat tttactgagg aatataaagg agctcaaaat ccgatttata tgagaaatcc
     1441 aaacttggaa gagtttgctc aatggcagaa ggctgcaaaa ggcttgatta caaaaattgc
     1501 tttggcacca gaacgtgagg gagttgagga atttgtctca gcaatcacaa agcaaggtgt
     1561 tacggtagct ttaggtcatt ctaatgggac atataaagaa gctaaaaagg cagtgaaggc
     1621 gggtgcctca gtttgggtac atgcgtataa tggtatgcgt ggtttgacac accgagaacc
     1681 aggtatggtt ggtgcggttt ataatttacc taatacttat gctgaactca tttgcgatgg
     1741 tcatcacgtt gatcctgttg cttgtgacat tttaatgaca caaaaaggcc ataatcatgt
     1801 ggcattaata acagattgta tggcagctgg aggtgcccct gatggagatt atatgttagg
     1861 ggaattgccc gtggttgtgt ctaatggaac tgcgcgtttg aaagatggag ggaatttagc
     1921 tggttctatt cttaagctca aagatggatt aaaaaatgtt gtagaatggg gaattgctac
     1981 tccggaagag gcagtgcgta tggcgagtta tgttccagca gtgtctgtag gcattgataa
     2041 tgtctgtgga cagataaaag aaggctatgc agcggatttt attgtattag ataaacatct
     2101 agatttagtt gcaacatacc ttgatggaga aaaacgttgg caatcttgaa agaactagtt
     2161 tttactagtt ctttttaatt tattaatttt taatgaaata ta
//
LOCUS       DATROQ010000165         2193 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.166, whole genome shotgun sequence.
ACCESSION   DATROQ010000165 DATROQ010000000
VERSION     DATROQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.166"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(6..268)
                     /locus_tag="VBC94_002028"
                     /pseudo
     CDS             complement(6..268)
                     /locus_tag="VBC94_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1037..1414)
                     /locus_tag="VBC94_002029"
     CDS             complement(1037..1414)
                     /locus_tag="VBC94_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000479581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980249.1"
                     /translation="MFLNTRNFGHVQSIYYTEMAKMYIMIMLIIFIVFSGIYYFYYLP
                     KNQGKKWKFNQWKLQKGKKEQLKINYGIIFGLVMLSPVFLVGSIENIFGLIGGVLFTA
                     TFTALIVDSLYAAYYIHKYPEYE"
     gene            complement(2009..>2193)
                     /locus_tag="VBC94_002030"
     CDS             complement(2009..>2193)
                     /locus_tag="VBC94_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980250.1"
                     /translation="YISNFIVTFGLILLVTPFLKYGIYNSFGVLISLLFTATLPSLIV
                     DSLYAAFYVHKHPEYE"
BASE COUNT          787 a          379 c          237 g          790 t
ORIGIN      
        1 tatagttacg gtatacatat caatgtaaga ctcaagattt ttaaattcat attatttgta
       61 ggttctttat cagaccaagc taatactctt tcatttgaag taatattatt atttgtatga
      121 tattcaagat tagctatttt tgatttcaca aaaaaacctt cttctacttt tttagactga
      181 ttctttcttc ccacttcttc gtatcaatat catatatttg aagaactaaa ctctcagagt
      241 ttccagtcaa ttttagtaat aatcccaaaa ctaatccttc ctacaaacca acggataatt
      301 ttatttcaac tggtcctggt tttaatttta cttgtgcaat ttcgcttgtt ataccaacta
      361 ttgatatcgc cgatactcct attcctaatg atgcgcacgc atcattagtt aaacaaagta
      421 ctttaaatcc tacagagggt cattccatca attaatggca taggttttgg cactagaaac
      481 tgaatatgaa ttctattatc tattaaaaaa atcaatatca ccagagtatt tataaaacat
      541 aacgataaaa aatcaaaaaa tatttttact atttccctta atttcatgag atttaatttc
      601 cctttttgtg tattatttct attctacaat agtcttaccc ttagaaatat ttttccattt
      661 ttcaatgata cgagctactc tttcttactc ctcgtcatgg tacctcccaa aattacttca
      721 tcaatatctt ctcattgaaa taatatactt gttctaaatt gacctctctg gaaaaatcct
      781 gccagataat caaaatataa tcttgatcaa taatatttgc tcatgaaacg gctccacaaa
      841 atttcttaca tctttaacat tttcatagag tgcttttact ccacaatggc caaagtacct
      901 ataacaactg ctactactgg tacaaattgg agttgttatg attactacaa aaacactctc
      961 agcaagcgta gagactattc tacccacttc attttctcta gatatttatt gctcctacct
     1021 taaattaatt aatttatcac tcatattcgg gatatttatg aatataatat gctgcataaa
     1081 gggaatcgac aatcagagct gtaaaagttg ccgtaaataa tacacctcca atcaaaccaa
     1141 aaatattttc aatactacct acaagaaaga ctggagaaag catcactaaa ccaaaaataa
     1201 ttccataatt aatttttaat tgttcctttt ttcctttttg taatttccac tgattaaatt
     1261 tccatttttt cccttgattt tttggtaaat aatagaaata atatattcca gaaaatacaa
     1321 taaaaataat taacattatc ataatataca tttttgccat ctcagtataa taaattgatt
     1381 gaacatgtcc aaaatttcta gtatttaaaa acattgccaa gcctataaaa tcaaaaccta
     1441 caataagtgt taggaatact aatgataata aaatataaga aattttttta aaaaattaga
     1501 tattttttca aaaaaagcga aataattatt gagaccaaat taacaaaaat gcatacagaa
     1561 aatatgaatt ttataaaacc taaaggatca ccaaaattac ttgcatataa ccctatgcaa
     1621 gatataaata gtccaatcca tggaaaaatt ttaatatatg aatattctat ttcaaactca
     1681 attaacggtc catggtatct atgattgctc tgtttcaaaa attttaccct ccaaataaat
     1741 cttttaattc ttttttgcaa aaccagttac tgattgatct ggtttgtttt gtccagtcca
     1801 atcacaatca agtgcccaat aaccttccag cacctaataa agtatctact ctagaatcta
     1861 ttgtgaaatt tctaattttt tgtctaaaat agcttgttat cctctatctt taaagtctct
     1921 gacttatcaa gcgaagatgt tttatctgaa tttatagttt ctgtcttata aaaggtatca
     1981 tgtgttttcg atgttcttta taagttgatt attcatactc aggatgctta tggacataaa
     2041 atgccgcata aagtgaatca acaattaatg atggtaaggt tgctgtaaat agtaaactga
     2101 ttaaaactcc aaaactgtta tagataccat actttaaaaa tggagtaact aacaatatca
     2161 gaccaaatgt aacaataaaa ttacttatgt att
//
LOCUS       DATROQ010000166         2103 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.167, whole genome shotgun sequence.
ACCESSION   DATROQ010000166 DATROQ010000000
VERSION     DATROQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.167"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(34..1131)
                     /locus_tag="VBC94_002031"
     CDS             complement(34..1131)
                     /locus_tag="VBC94_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6980251.1"
                     /translation="MRIIAITEKVIKELFRDKRTLAMMFLAPILIMFLMNVMFSANSN
                     TKVKIGTINVNTKVVSNLDNIKHIQVRSFKFNSSAKKALKSNKIDALISEDNKSYTVF
                     YANTDSSKTTLTRQAFKTAVNTMNSKELISQVKILANKNPKLAQSLQTRSKYIKEKYN
                     YGNKNTGFFAKMIPILMGFMVFFFVFLISGMALLKERTSGTLDRLLATPVKRSDIVFG
                     YMLSYGILAIIQTIVIVLSTIWLLDIQVVGSIFSVIIVNFILALVALSLGILMSTLAK
                     SEFQMMQFIPLIIMPQLFFSGIIPLENMASWAQTVGKILPLSYSGDALTKIIMYGQGL
                     PNVSSNLLVLLLFLIILTIANIFGLKRYRKV"
     gene            complement(1134..1850)
                     /locus_tag="VBC94_002032"
     CDS             complement(1134..1850)
                     /locus_tag="VBC94_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6980252.1"
                     /translation="MKKVIDLKKLQKAYASETVLNNINLEVFKGEIIGLIGPSGAGKS
                     TLIKTMLGMEKADKGTALVLDTQMPDRNILNQIGYMAQSDALHESLTGLENLLFFGKM
                     KGIQKTELKQQITHISKVVDLENQLDKFVSGYSGGMKRRLSLAIALLGNPTVLILDEP
                     TVGIDPSLRRKIWQELINIKDEGRSIFITTHVMDEAELTSKVALLLRGNIIAFDTPLH
                     LKKQFNVSTIEEVFLKAEGE"
BASE COUNT          695 a          328 c          334 g          746 t
ORIGIN      
        1 tatttttcat acttagttcc ttttcaaatg gtattatact ttacggtatc gttttagtcc
       61 aaagatattt gcgatggtta aaataattaa aaatagaaga agtaccagaa gatttgaaga
      121 aacgttaggt aatccttgac cgtacataat tatttttgta agtgcatcac cagagtagga
      181 tagcggtaga attttaccga ctgtctgagc ccaacttgcc atattttcta gagggattat
      241 tcctgaaaag aataattgag gcattataat taagggaata aattgcatca tttgaaattc
      301 tgattttgct agagtagaca ttaagattcc taatgataag gcaaccagag ccaaaataaa
      361 attgacaatg atgacactaa aaatactacc tacaacttga atatctagta gccaaatagt
      421 tgataaaacg ataactatcg tttgaataat tgctagaatg ccgtatgata acatatatcc
      481 aaatacaata tcactacgtt taacaggagt tgcaaggagt cgatcaagtg taccagaagt
      541 acgctctttt aataaagcca ttccagaaat taaaaaaaca aagaagaaga ccataaatcc
      601 cattagtatt ggtatcattt ttgcaaaaaa gcctgtattt ttatttccgt aattatattt
      661 ttctttgata tatttggagc gagtttgtaa ggattgtgct agtttcggat tcttattagc
      721 taaaatttta acttgcgaaa tcagttcctt actgttcatt gtattaacag cggttttaaa
      781 agcttgtctt gttaaagtcg tctttgaaga atctgtattc gcatagaaga cagtataaga
      841 tttattgtcc tccgaaataa gagcatcaat tttatttgat ttgagtgctt ttttagcaga
      901 tgagttaaat ttaaatgatc tcacttgaat atgcttaata ttatctaaat ttgaaacgac
      961 cttcgtgtta acgttaatag ttccaatctt aacttttgta ttactattcg cagaaaacat
     1021 aacattcatc aaaaacataa ttaaaatagg tgctaaaaac atcatagcaa gtgttctttt
     1081 atcacgaaac agttctttta taaccttttc agttattgca ataattctca tgattattct
     1141 ccttcagctt ttaagaaaac ttcctcaata gtactcacat taaattgttt ttttaaatgt
     1201 aatggagtat caaaggcaat aatgtttcca cgtaatagta gtgcaacctt acttgttaat
     1261 tctgcttcat ccataacgtg ggttgtaata aagatagaac gtccttcatc cttaatatta
     1321 attagctctt gccagatttt tctcctcaag gatggatcaa ttccaacggt aggttcatct
     1381 aggattaaaa ctgtggggtt tccaagtagg gcgatggcta gagaaagccg tcttttcata
     1441 cctcctgagt aacctgagac aaatttatca agttggtttt ctagatctac tactttagaa
     1501 atatgagtta tctgctgttt taattcagtt ttttgaatac ctttcatttt tccaaagaat
     1561 aataaatttt ctaagccagt taaagactcg tgtaaggcat cagattgagc catatagcca
     1621 atttgattta aaatattacg atctggcatt tgagtatcaa gaacaagagc tgttccctta
     1681 tctgcttttt ccatgccaag catagtttta atcaaggtag atttccctgc tccagagggt
     1741 cctattaatc caattatttc tcctttaaac acctccaaat taatattatt taaaacagtt
     1801 tctgaggcgt atgctttttg tagttttttt aaatcgatga cttttttcat gataactttt
     1861 tgctcctctc tcagtgagtg agtgtttact caccaatttg atatcatata ttatagtgtg
     1921 taaagacagg cttgtcaata aaagaattga caggaagtta atatactata atagtcaata
     1981 aaatggctta tttgagataa ctaatttttt atcttaaaaa tcagttgcat ggagagcatt
     2041 cgtgaaaatg ggcacagaat aaaacgacta agaatatcct tgtcgtttta ttttttatat
     2101 agt
//
LOCUS       DATROQ010000167         1934 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.168, whole genome shotgun sequence.
ACCESSION   DATROQ010000167 DATROQ010000000
VERSION     DATROQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.168"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            182..988
                     /locus_tag="VBC94_002033"
     CDS             182..988
                     /locus_tag="VBC94_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6980253.1"
                     /translation="MSKKIILGILSLLSVVTLVACGSSDKQLQDKVEKKGKLVLAVSP
                     DYAPFEFKALVNGKDKVVGADIELAQAIADELHVKLEVSPMSFDNVLSSLQTGKADMA
                     ISGLSYTKERAKVYDFSKPYYTTENAVLVRKSDLGKYTSIESLKGKKIAVQKGSIEEG
                     VSKKSFKNSHVVSLTAMGEAISELKSGKVEAVDLEKPVAEGYLAQNPDLVLAKFALKT
                     GEGDAKAVALPKDSGKMLKTVNKVIKRLEKEDKYKVYISDAAKLTGNQVE"
     gene            1071..1574
                     /locus_tag="VBC94_002034"
     CDS             1071..1574
                     /locus_tag="VBC94_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEO6980254.1"
                     /translation="MVQGSINIEKYKDLALQKQGEHRKFLASLKKKAPKNLDKIVQQI
                     HQEVFQEIDCTACANCCKTLGPLFTEADISRISKHFRMKLSAFEDLFLEVDEDGDKVF
                     KAMPCPFLGDDNLCSIYDVRPKACREFPHTDRKKIYQINNLTIKNTLTCPAAYLFVEK
                     LKSRIGK"
     gene            1678..1854
                     /locus_tag="VBC94_002035"
     CDS             1678..1854
                     /locus_tag="VBC94_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEO6980255.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
BASE COUNT          704 a          262 c          374 g          594 t
ORIGIN      
        1 ttttaagaat tggaatactt ttaaaaggtg attgttttaa ttagttatag ttgttattaa
       61 aaatcgtcta acagcaagca tagttatcgg tgtaatttac tgaattttta ttcaaaaaaa
      121 cttgacttat gtataaagat gatttatact tgttcctatc ataataaaag gaagatagtt
      181 tatgtcaaaa aaaataatat taggaatttt atctctttta tctgtcgtta ctttggtggc
      241 gtgtggttca tcagacaaac agctacaaga taaagttgag aaaaaaggga agttagtttt
      301 agcggtgagt ccagattatg ctccctttga gtttaaagcg cttgttaatg ggaaagataa
      361 agttgtcggc gcagatattg agttagctca agctattgct gatgagcttc atgtcaaact
      421 agaggtatct cctatgagtt ttgacaatgt tttatcaagt ttacaaacag gtaaggctga
      481 tatggctatt tctggattat catatactaa agagcgtgct aaagtctatg atttttcaaa
      541 accttattac actacagaaa atgcggttct tgttaggaag tctgatttag gaaaatatac
      601 ttcaatcgaa agccttaaag gtaaaaaaat agcagtacaa aaaggaagta tcgaagaggg
      661 agtttctaag aaatcattca aaaattctca cgtcgtgtca ctgacagcga tgggagaagc
      721 gattagtgag ttaaaatcag gcaaggtaga agctgttgat ttagaaaaac ccgtagctga
      781 aggttacttg gcccagaatc cagaccttgt tcttgctaag tttgccctta aaacaggaga
      841 aggggatgct aaggctgtag ctttacctaa agacagtgga aaaatgttga agactgttaa
      901 caaagtgata aagagacttg aaaaagaaga taaatataaa gtttatattt cagatgcggc
      961 taaattaact ggcaatcaag ttgaataata ttaaaaagcc tagtcatagg cttttttgga
     1021 tgttataata tggcttgact aagaataata gcataaaggc agaggaatat atggttcagg
     1081 gttcaattaa tattgaaaaa tataaagatt tggcgctcca aaaacaaggc gagcatcgta
     1141 aatttttagc aagtttaaaa aagaaagctc ctaaaaactt agataaaata gtccaacaga
     1201 tacatcaaga ggtttttcaa gaaattgatt gtacagcatg tgctaattgt tgtaaaactt
     1261 tgggtcctct atttacagaa gctgatattt ctaggatttc taaacatttc cggatgaagt
     1321 tatcggcttt tgaagattta tttttagaag tagatgaaga tggagataaa gtatttaaag
     1381 cgatgccttg tccgttttta ggcgatgata acttatgtag tatatacgat gtcagaccta
     1441 aagcttgtcg tgaatttcca catactgatc gtaagaaaat ctatcaaatt aataacttaa
     1501 cgataaaaaa tactcttact tgtccagcgg catacctttt tgtggaaaaa ttgaaatcac
     1561 gtatagggaa ataataaccc agagaaagta tcataaaaat attgacaatc tgcttttatt
     1621 tgggtatcct agtaagtgaa cgtgaaaatg ttacaatatc ttgaggaggt gaaacatatg
     1681 tcaaaaacag tagtacgtaa aaacgaatca cttgacgatg cacttcgtcg tttcaaacgt
     1741 tctgtgacta aagctggtac tcttcaagaa tcacgtaaac gtgaattcta cgaaaaacct
     1801 tctgtaaaac gtaaacgtaa atcagaagca gctcgcaaac gtaaaaaatt ctaattagat
     1861 agaatatgaa aaaacagttc agaattgagc tgttttttca tatattataa ctataagtta
     1921 ataaaaaaac tcgg
//
LOCUS       DATROQ010000168         1915 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.169, whole genome shotgun sequence.
ACCESSION   DATROQ010000168 DATROQ010000000
VERSION     DATROQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.169"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            241..648
                     /locus_tag="VBC94_002036"
     CDS             241..648
                     /locus_tag="VBC94_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980256.1"
                     /translation="MQKSSKIILLIVALLLLIFSGGFYMKEQQRKEELKRNREYEVSL
                     VKALKNSYSDIKEIQISNPITTTMPGDWRSTVKIIFSDNDEITYRITHNKDDEINRSG
                     SINEKEHLFLSKKYGRTKNHILVEFSTGEKEKR"
     gene            648..1235
                     /locus_tag="VBC94_002037"
     CDS             648..1235
                     /locus_tag="VBC94_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980257.1"
                     /translation="MTYSYSEYDLNRLNQGENVYSVNPNYVKQNGGKIITANPKKSNE
                     TNLIVTKTGKAFKVLKTSPDDMSGYQGMAVAPIIKGKVDYNSVAVISAATDSSNYKDL
                     IGAVSSAQPHQSSTQLKSADKFLKDVQSHDKWTVTQLSGYSQSAYMLKLGAKYHIPTT
                     VFNGWFRYSTLNEDEKNSWLSILNILLIFDIKRIM"
     gene            1181..1861
                     /locus_tag="VBC94_002038"
     CDS             1181..1861
                     /locus_tag="VBC94_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980258.1"
                     /translation="MAKHPEYFANFRHKEDNVTWWNDFNKLDDKGYGTVKWVNGKSHK
                     IESWKFTDDGQLKDEKGNIVNPKSPAVQSVLYEEVHFQKAKAKLKKSGGKLSHSEKVY
                     LDSEQAIFIANGLTTASQTASDDIKKNAELAKEKASELFAKTKVMPPGITDLSPEELA
                     DAYSAGGVREDTIVTPIETFFDEKVTNAQEITTSYTNLQKQIESGVQKLLEEDSKLAG
                     EFKEWSQY"
BASE COUNT          774 a          248 c          338 g          555 t
ORIGIN      
        1 ctattgttta taatattaca cataatttgg aatcgaaaaa aaattatagc ggaaaattta
       61 atgaagaaaa tatgaatttt tttgattcaa gaattggtaa aacaaaaaaa actataaaaa
      121 ttattttttc agatggtcag gagaaatttg aataatggta ttttcatata ttggcttatt
      181 caaattccta agttgatata ttatagctag atgatgtaat tgtttcataa aggagtgaaa
      241 atgcaaaaga gttctaaaat aatactacta atagttgccc tattactact tatttttagc
      301 ggaggatttt atatgaaaga acaacaaaga aaagaagaac taaaacggaa tcgagaatat
      361 gaggttagtc tagtcaaagc attgaaaaat tcatattcag atattaagga gattcaaatt
      421 tccaatccaa taacaacaac aatgccaggt gattggcgta gtactgtgaa aattattttt
      481 tcagataatg atgaaatcac ttaccgtata acccataata aagatgatga aattaatcgc
      541 agtggatcaa taaatgaaaa agaacattta tttttaagta aaaaatatgg aagaacaaaa
      601 aatcatattt tagttgagtt ttctacagga gaaaaggaaa aaagataatg acatattctt
      661 atagcgagta tgatttaaat agattgaatc aaggtgagaa cgtatatagt gtaaatccaa
      721 actatgtgaa acaaaatggc ggaaaaataa taacagctaa tccaaaaaaa tctaatgaaa
      781 caaatttaat agtaacaaag acgggtaaag cgtttaaggt attaaaaact tctcctgacg
      841 atatgtctgg ctatcagggt atggcagttg caccaattat caagggtaaa gtagattata
      901 acagtgttgc agttatttcc gccgcaacgg attcaagtaa ttataaagat ttaattggcg
      961 cagtttcatc tgctcaacca catcaaagtt cgactcagtt aaaatcagct gataaatttt
     1021 taaaagacgt acagtcacat gacaaatgga ctgtgactca gctttcaggc tattctcaat
     1081 ctgcttatat gctaaaatta ggtgctaaat atcatatccc gactacagta tttaatggat
     1141 ggtttaggta tagcacttta aatgaagatg aaaaaaattc atggctaagc atcctgaata
     1201 ttttgctaat tttcgacata aagaggataa tgtaacttgg tggaacgatt ttaataaact
     1261 agatgataaa ggttatggta ccgtgaaatg ggtaaatggt aaaagtcata aaatagagag
     1321 ttggaaattt actgatgatg gtcagttaaa agatgaaaaa ggtaacatcg taaatcctaa
     1381 gtcaccagct gttcagtctg ttctttatga agaagttcat tttcaaaaag ctaaagctaa
     1441 attaaaaaaa tctggaggca aacttagtca tagtgaaaaa gtatatttag attctgaaca
     1501 ggctatattt attgctaacg gtttaaccac ggcatctcaa acagcttctg atgatatcaa
     1561 gaagaatgct gaattagcta aagaaaaagc atcagaacta tttgcaaaaa ctaaagtgat
     1621 gcctcctggt ataacagatt taagtccaga agaattagct gatgcatata gtgcgggtgg
     1681 agttagagag gacaccatag taacaccaat tgaaactttc tttgatgaaa aagttactaa
     1741 tgcacaagag atcacaactt catatactaa tcttcaaaaa caaattgaat ctggtgtcca
     1801 aaaattgcta gaagaggact ccaaattagc aggagaattt aaggaatggt cacaatacta
     1861 gatcaaaatg aaaacaaaat taatagtcta aaaataaacc taaggaaatt ggaag
//
LOCUS       DATROQ010000169         1792 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.170, whole genome shotgun sequence.
ACCESSION   DATROQ010000169 DATROQ010000000
VERSION     DATROQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.170"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..284)
                     /locus_tag="VBC94_002039"
     CDS             complement(<1..284)
                     /locus_tag="VBC94_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar synthetase-like protein"
                     /protein_id="HEO6980259.1"
                     /translation="MRTYITNLNGHSITSTAQIAQNMVTDIAVSLGFRELGIHSYPID
                     TDSPEEMSKRLDGICSGLRKNDIVIFQTPTWNTTTFDEKLFHKLKIFGVK"
     gene            complement(338..505)
                     /locus_tag="VBC94_002040"
     CDS             complement(338..505)
                     /locus_tag="VBC94_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980260.1"
                     /translation="MPKRNELLNKEIKMSIDKLRYKEPESEHDKRPTFYLVVLILVTV
                     AVILSLFKYFL"
     gene            complement(498..>1792)
                     /gene="gtfB"
                     /locus_tag="VBC94_002041"
     CDS             complement(498..>1792)
                     /gene="gtfB"
                     /locus_tag="VBC94_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEO6980261.1"
                     /translation="KDLYYSLITSGLHGNAVVINDDGFLPQNINSPYSFFCNMEGKNG
                     NPLYFNQVPLPDLWEIKGNNIEAEIWDFSIKRAKIFYQEPKYKRQVKNIDWFDNNKKV
                     RYTDHYNRFGWCFARTHFDKNQNVTTKSYFDKDGKEVIVENFRTGVIILNWLNKDYFF
                     DNRVAFLNFYFSLMGWNLSRIWYNSLSTPFFVSYRMTYPGEDILFWQEDIEDTIPANM
                     RVLLESTNTRTQKVIVQKKNTYHKIKSMLPKEQQEKIGYLGFIYPNKKNNKGRKDIFI
                     LTNSDQIEHLEVLVHHLSDYHFHIAAYTEMSFKLMSFSQEQNVTLYPNISRTDLDNLF
                     EICDIYFDINHGNEVDDVIRRAFEYNHLIFAFDNTCHNRELVLDSNIISHTTCEQLIN
                     LMKNLSGSIMYLLEQQREQTSNETKERYKEILGGYGNA"
BASE COUNT          596 a          308 c          231 g          657 t
ORIGIN      
        1 atctttacac caaatatttt taatttgtga aataattttt catcaaaagt tgtagtgttc
       61 catgtaggtg tctgaaatat gacaatatca ttttttctaa gtccggaaca gattccatct
      121 aaacgcttac tcatttcctc aggagaatca gtatcaatcg gataagaatg tattcccagc
      181 tcacgaaaac ctaagcttac tgctatatct gttaccatgt tttgagctat ttgtgctgta
      241 ctagtgattg aatgtccatt caagtttgta atatatgtcc tcacacactt cccctatcta
      301 aaaaacaaca ggcttctttt ctaccattaa taggaatcta taaaaaatat ttaaataacg
      361 acaatataac tgctacagta acaagtataa gtactaccaa ataaaaagta ggtcgcttgt
      421 catgttcact ctctggttct ttatatctaa gtttatcaat actcatttta atttctttat
      481 tgagtaattc atttctttta ggcatttcca taccctccta atatttcttt ataacgctct
      541 tttgtttcat tacttgtttg ttctctttgt tgctctagca aatacataat ggagcctgat
      601 aaatttttca ttaaatttat caattgttca caggttgtgt gagaaatgat attgctatct
      661 aatactaact ctctgttatg acaggtatta tcaaaagcaa agataaggtg attatattca
      721 aatgcccttc taataacatc atcaacttca tttccatgat taatgtcaaa ataaatatca
      781 cagatttcaa ataagttgtc taaatcagtt cttgaaatat tcggatatag cgttacattt
      841 tgttcttggc taaatgacat caacttaaaa gacatttctg tgtacgctgc aatatgaaaa
      901 tgataatcag acaagtgatg aaccaacact tctaaatgtt caatttggtc agagtttgtc
      961 aaaataaaaa tatctttcct acccttattg ttctttttat taggatatat aaagcctaaa
     1021 taaccaattt tttcttgttg ttctttaggt aacattgact ttattttatg atatgtattt
     1081 ttcttttgaa caattacttt ctgagttcta gtattcgtac tttctaataa cacgcgcata
     1141 ttagcaggaa tagtatcctc tatatcttct tgccaaaata aaatatcttc tcctggataa
     1201 gtcatccggt aagaaacgaa aaatggagtt gataaggagt tgtaccatat tcgagacaag
     1261 ttccaaccca taaggctaaa ataaaaattt aagaatgcaa ctcgattatc aaaaaaataa
     1321 tctttattta gccaattaag gattataact cctgttctaa aattttcaac aataacttct
     1381 ttaccatcct tatcgaaata agattttgta gttacatttt gattcttatc aaaatgtgta
     1441 cgtgcaaaac accatccgaa gcgattataa tgatctgtgt atctcacttt tttattattg
     1501 tcaaaccaat caatattttt tacttgacgt ttatacttcg gctcttgata aaaaatttta
     1561 gctcttttaa tactaaaatc ccaaatttca gcctctatat tatttccctt tatctcccaa
     1621 aggtcaggga gtgggacttg gttaaaataa agaggattcc catttttccc ttccatatta
     1681 caaaaaaatg aataaggaga atttatattt tgtggaagaa aaccatcgtc attaatgacc
     1741 acagcatttc catgtaaacc tgaagtaatt aaagaataat atagatcttt gc
//
LOCUS       DATROQ010000170         1792 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.171, whole genome shotgun sequence.
ACCESSION   DATROQ010000170 DATROQ010000000
VERSION     DATROQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.171"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..1770
                     /locus_tag="VBC94_002042"
     CDS             <1..1770
                     /locus_tag="VBC94_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEO6980262.1"
                     /translation="LKEDFFLTLTSLKNQFWVYIGTGILIQTLVAYLVKGILSFIFRR
                     ILILSNTPAVTKDNWTLLFNHPLALLLFILYIVILIGFIYTEFAIYTIAILKTEFSLS
                     IKKWLTIFPKKIKSLLGPQLIWVSIYLLLTIPLANLGLRSSILEHLKIPDFISGELTK
                     TVFGKIGYSTLLLLICYLNLRLIYFLPLTILTDYNAKEALLESWKLSRGKRQWRLLSK
                     IILTSLFISVIGTVALAVVAGTFSFIDHSGNNFPLQATFYNLLKSILFAVTLLIKCLI
                     ISHLLADIKQNKSIVQQWRAWKGVHHKQKFKHIACVILALAIGTTALKNAIALALLQD
                     NISIKKELIAHRSDTSHGVENSIEALKAAHKAKADYSEMDVIMTKDHKLVVIHDDNLK
                     RLSGMNKDVSKLTLDQVTKIPIHQGHFASYIPSLAEFMKTAQSLDQKIMIELKPYNQN
                     LDIYADEFIKEFKELRLSTKHKVMSLNLTLIEKVEKKLPQLDTGYLIPLHWGTLQNHN
                     VDFYGIEEFSYNDWIAYLAQEYNKQLYVWTINRDNLMIRYLQSPVNGIITDKLNLFKV
                     INKDIKNSPNYYQRALQLIDSEG"
BASE COUNT          611 a          326 c          261 g          594 t
ORIGIN      
        1 ttaaaagaag atttttttct aaccttaacc tctttaaaaa atcaattttg ggtttacatt
       61 ggaactggta tcttaatcca aactttagtt gcttatttag taaaaggaat tctgagtttt
      121 atctttcgtc ggatattgat attatcaaat actcccgcag tgactaaaga caattggact
      181 ttgcttttta atcatccctt agctttatta ctctttatac tttatatcgt aatcctaatt
      241 gggttcatct atactgagtt cgctatctat actattgcca tactgaaaac tgaattttct
      301 ctcagtatca aaaaatggct aacaatcttt cctaaaaaaa taaaatcact tttaggacca
      361 cagctaattt gggtatcaat ctacttactt ttgacaattc ctctagctaa ccttggtcta
      421 aggtcaagta tattagagca tcttaaaatt cctgacttta tttctggcga gttaacaaag
      481 acagtctttg gaaagatagg ttactctacc cttttactgc ttatttgtta cttaaacctt
      541 cgattaattt attttctccc gctgaccatt ttaactgact acaatgctaa agaagcactt
      601 ttagagagtt ggaaacttag ccgcggaaag cgtcagtggc gtcttctaag taaaattatt
      661 ttaaccagtt tatttatatc tgtaatcgga acagttgcac tagctgttgt tgctggtaca
      721 ttttcattca ttgatcattc tggaaacaat tttcctcttc aagccacttt ctataacctt
      781 ttaaaatcaa tactttttgc cgtaacactt cttattaaat gtttaatcat aagtcaccta
      841 ttagcagata ttaaacaaaa taaaagtatt gttcaacaat ggagagcttg gaaaggcgtc
      901 caccataagc aaaaatttaa acacattgct tgtgttattc tagctctagc tattggtacc
      961 actgccttaa aaaatgctat agctctggct ctcttacaag ataacatttc aattaaaaaa
     1021 gaattaatag cccatcgtag tgatacatca catggcgttg aaaattctat tgaagcatta
     1081 aaagcagccc ataaagcaaa agcagattat tcagagatgg atgttatcat gacaaaagat
     1141 cataaattag tagtcattca tgatgataat ctcaaacgac tctctggtat gaacaaagat
     1201 gtttctaaac taactttaga tcaagttaca aaaatcccaa ttcaccaagg gcattttgct
     1261 agttacattc catcattggc agaatttatg aagaccgctc agtcactaga tcaaaaaatc
     1321 atgattgaat tgaaaccgta taatcaaaac ctcgatatct atgcggatga atttatcaaa
     1381 gaatttaaag agcttcgtct atcaaccaag cataaagtaa tgtctctcaa cttgacactc
     1441 attgaaaagg ttgagaaaaa attgccacaa cttgatacag gttacctaat cccactacat
     1501 tggggaacgc ttcaaaatca taacgttgac ttttatggga ttgaggaatt ttcatacaat
     1561 gattggattg catatctcgc ccaagaatat aataaacagc tctatgtttg gactattaac
     1621 cgtgataacc ttatgatacg ttatttacaa agcccagtta atggaatcat cacagataaa
     1681 ctcaatcttt tcaaggtcat taataaagat atcaaaaata gccctaatta ttatcaaaga
     1741 gctctccaac ttattgatag cgagggttaa attaaataaa aagcttagta ga
//
LOCUS       DATROQ010000171         1741 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.172, whole genome shotgun sequence.
ACCESSION   DATROQ010000171 DATROQ010000000
VERSION     DATROQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.172"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            231..707
                     /locus_tag="VBC94_002043"
     CDS             231..707
                     /locus_tag="VBC94_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980263.1"
                     /translation="MYIIPAGYSPERLDSEVLTAKIEDDEALLRKIQDLKDHLDKNTK
                     ILAYAFDGLEVRVKARLRKNRKKLKNLIEFNAKSTSVFNELKDYSNAVSSGLTFVSKG
                     FSSFNGQFSSASNLDWQKTIKTGMRKREEDAKQLRKKALEDSNKLRKIASKRGYFE"
     gene            963..1300
                     /locus_tag="VBC94_002044"
                     /pseudo
     CDS             963..1300
                     /locus_tag="VBC94_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          635 a          217 c          328 g          561 t
ORIGIN      
        1 aaaaaagata aatatattgc attgaattgg ttgtgtgcga tagccacgct atactattaa
       61 gttattacaa actatgaatt acacctcaaa tgttggagat aaaacctatc gtttggggtg
      121 ttttttggta aaatagaatg aaaagagtta ctctgtgtca ttactaagtt aaaaaattat
      181 actaacttaa ttattaacgt tttattgact gagttactct tttttaaaaa atgtatatca
      241 ttccagctgg ttactcacct gaacgtttag actcagaagt ccttacagca aagattgaag
      301 atgatgaagc cttattgcgg aaaattcaag accttaaaga tcatttagat aaaaatacca
      361 aaatcttggc ttacgctttt gatggcttgg aagtcagagt gaaagctaga ctaagaaaaa
      421 atcgtaagaa actcaaaaac cttatagaat tcaatgccaa atctacctca gtctttaatg
      481 aattgaagga ttattcaaat gctgtatcgt ctggtttaac ctttgtatct aaaggcttta
      541 gtagtttcaa tggccagttc tcttcagcct caaatcttga ttggcaaaag actattaaga
      601 caggaatgag aaaacgagaa gaagatgcta aacagttgag aaaaaaagct ttagaagatt
      661 caaataagct cagaaaaatt gcatcaaaaa ggggttactt tgaataatgc agttgaattt
      721 gctaaagaac agaacaaacc cctaaaaata aaaagataac catagataaa tatggtcgtg
      781 taaaaatgga taaaactttt ctttatcgaa aagctaaaga tggaaaatct gcatatattt
      841 attctgaagg gagtgatcaa tataaagaac taactgggaa agaatttgat cctaaaacta
      901 caaatattat gaaaggtaga aaattaggga cagatggtaa gatatcaatt gattttggtg
      961 agattagtaa ctcaggaaaa aatgcagcta ttaagtccct taaaaatgaa agtccttgaa
     1021 ttaaatccca aaaatgcttt taaaggatgg aatgagttaa gcaaaatagc taaaactggg
     1081 aaagtactag gatggacagg taaaattgct aatatttaca tgaatggaaa aaaagatctt
     1141 tttgatgata aaacatcttc aatagatcaa aaaatcaaaa actttgctat aggttcaggt
     1201 gtagatgcat tgtccggtgc agcaataggg actttggtag gaggacctgt gggaaccgtt
     1261 gttggagcag gtgcagggct ggcatttgat tttgctttga attttaaagt taaggagttg
     1321 ggtaatgaat caattacagg ttttgctaaa aaaggtttaa aaaatcttgc aggaggatta
     1381 ggattttgaa aaaaagtata aatgtaagta ggtatcatgg acctttaatt gaatttgagg
     1441 ttgatgcaag ttatttaaga attttttcat atatcggttt acctttatca ttgttttggt
     1501 attttgttac tcataacagt gatgactttg gaatattaaa atcgatattt ttaatttgta
     1561 ttatagtcaa tttaatcggg ctcatattag gattaataca aaagtttact aaaaaactta
     1621 tcaaatctct atcatttttt gtactttctt agcgtttctt tcgattttct tagggttaga
     1681 tacaatatgt ttaatggtat ttatttcgga tgggtcccct gttgatgcga atacgttgta
     1741 t
//
LOCUS       DATROQ010000172         1523 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.173, whole genome shotgun sequence.
ACCESSION   DATROQ010000172 DATROQ010000000
VERSION     DATROQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.173"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            296..481
                     /locus_tag="VBC94_002045"
     CDS             296..481
                     /locus_tag="VBC94_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980264.1"
                     /translation="MIDLNDLHQQVTTGRIAKDNVKAIMKDGRITDYYIDGEPLQASE
                     TIVNMDLMTLLANVFEI"
     gene            588..797
                     /locus_tag="VBC94_002046"
     CDS             588..797
                     /locus_tag="VBC94_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEO6980265.1"
                     /translation="MPRKVNKVIYVLLALFLGEFGLHKFYAGKTGTGILYLIFCWTFI
                     PGFIGVVEGILAILKPADQDGNFYI"
     gene            1317..>1523
                     /locus_tag="VBC94_002047"
     CDS             1317..>1523
                     /locus_tag="VBC94_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
                     /protein_id="HEO6980266.1"
                     /translation="MENLSTRLVDKSIEAFIMGLEIYNKPTIKYRIEGFSFFICNAWE
                     LMLKAEMLNRNQSIYFKDNPDRTLS"
BASE COUNT          534 a          241 c          260 g          488 t
ORIGIN      
        1 cattaaagca tgatgaatta tctactaaaa tatatgaata tgcgttagat ttatatgatc
       61 tagtattgta tagagcagtt tcagaatatc aagcaataat agagaaagaa agaaattatt
      121 aaaatttaca cgtactcccc caaccgtcca cactataccc taaaagtatg gcgtgacttg
      181 aaaacacccc aaaacagaca ttactattag taaggtcgtt tcaaccgtcc acatgctaag
      241 aaaaaaatat atcaaccttt ccttactttc cctcacaaat tacgaataaa taagtatgat
      301 agatttaaat gacttacacc aacaagtaac cactggcaga attgccaaag acaatgtaaa
      361 agccatcatg aaagatggcc gtatcactga ttattatata gatggcgaac cactacaagc
      421 tagtgaaacc attgttaaca tggatttaat gactttatta gccaacgtat ttgagattta
      481 acttcaagtt ttttaaagcc cacgcgccag tattttcaag ggttttatct atataaataa
      541 aaccatggta tctgtattta aacgctttat acaaaggaga aaaatttatg ccaagaaaag
      601 taaataaagt aatttatgtt ctattagctc tgtttttagg tgaatttggc ttacataaat
      661 tttatgccgg taaaacagga acaggaatcc tataccttat tttctgttgg acatttatcc
      721 ctggattcat tggagttgtt gaaggaatct tagcaatcct aaaaccagcc gatcaagatg
      781 gaaatttcta tatataaaga attattatct tccacagctt ttactgtatt ccgtatctga
      841 aaaagattca cgctaattca gcaatcaagc tgttcttgct tttggttatc tggtagggtg
      901 gcaataaggt agagagcttc tgttccgata tttcctaacg tcgggaaatt tggtaattca
      961 tgagctattt tcatcattca ctttgcaaaa gtataatcga tttccaactt atgcaactac
     1021 tcctccaata aaagcaatgg cagttagaaa cgtttgtctc tttaatgatg tgataattta
     1081 gtattcttat cttcggggtg gggggtcgtg cgataaaaaa agcaaatatt tggacagttg
     1141 acccttccca ccggtttcaa aacttggatt gaacaacatt tttttatgat gggggtaacc
     1201 ttaaagatac taaattagaa aaaattctga aaacttgtgg ttaagtattt tgttgatgtt
     1261 aataaaattg tacagatcga tttcgaagcg cgtgaaaaaa actagaaagg aaatatatgg
     1321 agaatttaag tacaaggtta gttgataaaa gtattgaagc ttttattatg ggacttgaaa
     1381 tatacaataa accaacgatt aagtaccgaa ttgagggctt tagtttcttt atttgtaatg
     1441 cttgggaatt gatgcttaaa gctgaaatgt taaatcgtaa tcaatctatc tattttaagg
     1501 ataatcctga tagaacacta agc
//
LOCUS       DATROQ010000173         1457 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.174, whole genome shotgun sequence.
ACCESSION   DATROQ010000173 DATROQ010000000
VERSION     DATROQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.174"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(124..>1457)
                     /locus_tag="VBC94_002048"
     CDS             complement(124..>1457)
                     /locus_tag="VBC94_002048"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEO6980267.1"
                     /translation="GSSLASSQQLYKVLNIIKSDYTRRFIVVSAPGKRYEEDLKMTDA
                     LIQYYQNYINGKDIVKDQTWIINRYQEIISDLSLGSTIAEEITRSIEQLASLPIENNQ
                     FLYDCFLAAGEDNNAKLVATFFNQNDIPARYVHPNEAGIIVTKEPCNARIIPGSYDKI
                     ENLCLYNEVLVIPGFFGVTEDNQICTFSRGGSDITGSLIAAGIKADLYENFTDVDGIF
                     AAHPGVVKNPHAIPELTYKEMRELAYAGFSVLHDEALLPAYRGRIPLVIKNTNNPQQP
                     GTKIVLKHTRSNIAVTGIASDSRFASINVSKYLMNREVGFGRKVLQILEDLNISFEHM
                     PTGIDDLSIVLREKELTPIKEQEILNYLTRKLEVDYVDIQHNLSTIVIVGENMKSQIG
                     VTATATQALSREKINITMISQGSSEVSIMFVIDSKDEKRAIKALYETFFQK"
BASE COUNT          438 a          250 c          251 g          518 t
ORIGIN      
        1 tgataccaag tagttacgat taaaattgaa aaaactatcc tatgttctaa ataaaatcgt
       61 cctaagtaaa cttacttagg acgatttgtt atctatctaa tagtgtaagt agtgtaatag
      121 gtactatttt tggaagaatg tttcatatag tgctttaata gctctttttt catccttact
      181 gtctataacg aacataatgg agacttcgct tgaaccttgt gatatcatgg tgatattgat
      241 tttttctctt gataaggctt gtgtcgctgt tgcagtgact ccaatctgac ttttcatatt
      301 ttcacctaca attacgattg tagataaatt gtgttggatg tcaacgtaat ctacttctag
      361 tttacgagtt aggtaattta agatttcttg ttctttgatt ggtgtcaatt ctttttcacg
      421 taagacaatg gatagatcat ctatgccagt tggcatatgt tcaaaactaa tatttaaatc
      481 ctctaaaatt tgtagtactt ttcggccgaa acctacttct ctattcatta agtatttaga
      541 tacgtttatg ctagcaaaac ggctatcaga agcgatccca gttactgcta tgttactacg
      601 agtatgcttt aaaactattt ttgtaccagg ctgttgggga ttatttgtat ttttaataac
      661 aagagggatt ctgccacgat aggcaggaag taaagcttca tcatgtaaaa ccgaaaaacc
      721 cgcataggct aattcacgca tttctttata agtaagctca gggatagcgt gagggttctt
      781 aactacacct ggatgcgctg caaatatacc atcaacatct gtgaagttct cataaaggtc
      841 tgcttttatg cctgctgcaa ttagggatcc agtaatgtca gatccccctc ttgaaaaggt
      901 acaaatttgg ttatcttctg tgactccaaa aaatccaggg ataacaagaa cctcattgta
      961 tagacataag ttctcaatct tatcataact tcctggaata attcgtgcat tacatggttc
     1021 tttagttaca ataattcctg cttcgtttgg atgaacatat cttgcaggaa tgtcattttg
     1081 attaaaaaac gttgcaacta actttgcgtt gttatcttca ccggctgcta aaaaacaatc
     1141 atataaaaac tgattatttt ctatgggaag tgaagctagc tgctctatag agcgagtaat
     1201 ttcttctgca atagtgcttc ctaaagacaa atcactaata atttcttgat aacgattgat
     1261 aatccaagtt tggtctttaa caatatcttt accgttaata taattttgat aatattgtat
     1321 caaagcatct gtcattttta agtcttcttc gtagcgtttc cctggagctg aaacgacaat
     1381 aaaccgtcta gtgtagtctg atttgataat atttagtact ttgtataatt gttgggaaga
     1441 tgctaaagag cttcctc
//
LOCUS       DATROQ010000174         1437 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.175, whole genome shotgun sequence.
ACCESSION   DATROQ010000174 DATROQ010000000
VERSION     DATROQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.175"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..1128
                     /locus_tag="VBC94_002049"
     CDS             <1..1128
                     /locus_tag="VBC94_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEO6980268.1"
                     /translation="KSLCVYHENLSIFIFNQDIPQEWFLAMKDRVGQTGNQIQDVKLF
                     HDHLSPKWENKKLNHINYMTYARYFIPQYISADTVLYLDSDLVVTTNLDNLFQISLDN
                     AYLAAVPALFGLGYGFNAGVMVINNQRWRQENMTIKLIEKNQKEIENANEGDQTILNR
                     MFENQVIYLDDTYNFQIGFDMGAAIDGHKFIFDIPITPLPKIIHYISGIKPWQTLSNM
                     RLREVWWHYNLLEWSSIISSKKVFGLDHPIKTQNYRLNFLIVTTSDCIPSISELVTAL
                     PDCLFHIAAPTVMSDILISLLAFENIRLHKTYTPPIIDKLLAEADIYLDINVFGKMEG
                     ILQKMSQSEKPILTTRECNSQLVPETVFASIDDLINYLQSI"
     gene            1193..>1437
                     /locus_tag="VBC94_002050"
     CDS             1193..>1437
                     /locus_tag="VBC94_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 8 glycosyl transferase"
                     /protein_id="HEO6980269.1"
                     /translation="MEKAVALAVDSNYLDKALVTIKSICVYNRNITFYLFNQDTPVEW
                     VRNINRKLEPLGSKLINVKIYNYDIAHLTTFLTVSTW"
BASE COUNT          497 a          248 c          210 g          482 t
ORIGIN      
        1 aaatctctct gtgtctatca tgagaatctc tcaattttta tttttaatca agatattcct
       61 caagaatggt ttttagctat gaaagatagg gttggacaaa ctggaaatca aattcaggat
      121 gtaaagctct tccatgatca cttatcccca aaatgggaaa ataaaaagct taatcatatt
      181 aattatatga cctatgctcg ttatttcata cctcagtaca tctcagctga tacagtttta
      241 tatcttgact ctgacttagt tgttactact aatttagata acctctttca aatttcacta
      301 gacaatgcat atttagctgc agttccagct ctttttgggc ttggatatgg gtttaatgct
      361 ggagtaatgg taattaacaa ccaacgttgg cgacaagaaa atatgactat taaattaatt
      421 gaaaaaaatc aaaaggaaat tgagaatgcc aacgaagggg atcaaacaat tcttaatcgc
      481 atgtttgaaa atcaggtaat ttatttagat gatacctaca attttcaaat tggttttgat
      541 atgggagctg ctatcgatgg gcataaattt atttttgaca tcccaattac cccactccca
      601 aaaattattc actacatttc gggaatcaaa ccttggcaaa cattatcaaa tatgagactc
      661 cgtgaggtat ggtggcacta taatttactt gaatggtcaa gtatcatatc tagtaaaaaa
      721 gtatttggtt tagaccaccc aattaaaaca caaaattatc gtctcaattt ccttattgtt
      781 acaacttctg attgtatacc atctatctca gaattagtca ctgcccttcc agattgtcta
      841 tttcacattg cagcaccaac agttatgtct gacattctta taagcctatt agcttttgaa
      901 aatatccgcc ttcataaaac ttacactcca cctattattg ataaattatt agcagaagct
      961 gatatttatc ttgatattaa tgtttttgga aaaatggagg gcattctaca gaaaatgtca
     1021 caatctgaaa aaccaattct tactacaaga gaatgcaata gccagcttgt tccagaaaca
     1081 gtttttgcat ctatcgatga tttaattaat tatcttcagt ctatataaaa aaattggcat
     1141 ccattcacta tatacccaac attattctaa acacttaact aggagaattt aaatggaaaa
     1201 agcagttgca cttgcagttg attcaaacta cttggataaa gccttagtaa caataaagtc
     1261 tatttgtgtt tataatagaa atataacttt ttatttattc aatcaagata ccccagttga
     1321 atgggtacgt aatataaaca ggaaactaga gcctctagga tcaaaactga ttaatgttaa
     1381 aatatataac tatgatattg ctcatctaac gacttttcta actgttagta catggtt
//
LOCUS       DATROQ010000175         1334 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.176, whole genome shotgun sequence.
ACCESSION   DATROQ010000175 DATROQ010000000
VERSION     DATROQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.176"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..140
                     /locus_tag="VBC94_002051"
     CDS             <1..140
                     /locus_tag="VBC94_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEO6980270.1"
                     /translation="RVTFINYHSKALSYFIKYELINNSKEELFNRKVIEKIIRTSIEN
                     Y"
     gene            150..1136
                     /gene="dhaQ"
                     /locus_tag="VBC94_002052"
     CDS             150..1136
                     /gene="dhaQ"
                     /locus_tag="VBC94_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HEO6980271.1"
                     /translation="MRKTEHHKMEYVFETIKAITKTNPKVEQIKDLPAIHYLGQSEQV
                     IPILSGGGSGHEPAHFGYVGEGMLSAAISGPIFVPPCASDILETIRFINRGKGVFVII
                     KNFEADLEEFSQAIEQARQEGIPIKYIVSHDDISVETSNFKIRHRGVAGTVLLHKIIG
                     QAALEGASLDELEQLGLSLTTSMATLGVASKSATILGQHQPVFDLEEGYISFGIGIHG
                     EPGYRTMPFVSMEHLANELVNKLKMKLRWQDGEAFILLINNLGGSSKMEELLFTNAVM
                     EFLALDDLQLPFIKTGHLITSLDMAGLSVTLCRVKDSRWIDYLKHKTDARAW"
BASE COUNT          469 a          188 c          268 g          409 t
ORIGIN      
        1 aaagagttac atttattaat tatcatagta aagccctctc ttattttata aaatatgagt
       61 taataaataa ctcaaaagaa gaattattta ataggaaagt cattgaaaaa attatcagaa
      121 catcaataga aaattattag gacaaggata tgagaaaaac agaacatcat aaaatggaat
      181 atgtttttga aacaataaaa gcaatcacta aaactaatcc taaagtagag caaataaaag
      241 atttgcccgc tattcattat ttagggcaat ccgagcaagt aattccgatt ttatcaggag
      301 gaggcagcgg acatgagccg gctcactttg gatatgttgg tgagggaatg ctatcggcag
      361 cgattagtgg tcctattttt gtaccaccat gtgctagtga tattctagag actattagat
      421 ttatcaatcg aggcaaaggt gtctttgtta ttatcaaaaa ttttgaagct gatttggaag
      481 aattttccca agcgattgaa caggctcgtc aagaaggcat tccaatcaaa tacatcgttt
      541 ctcatgatga catctctgtt gaaacatcaa attttaaaat tcgtcatcga ggcgttgctg
      601 gtactgtgtt gctacataaa attattggac aagctgcttt agaaggagca agcttagatg
      661 aattagaaca gctaggacta tctttgacaa cttcaatggc tactttaggt gtagcaagta
      721 agtcagccac tattttaggt cagcatcaac ctgtttttga tttagaagaa ggttacatct
      781 catttggaat cggtattcat ggtgagccag gataccggac aatgcctttc gtgtccatgg
      841 aacatttggc aaacgaacta gttaacaaat tgaaaatgaa attacgttgg caagatggag
      901 aagcattcat tctattgata aataatcttg gaggaagttc taaaatggag gaattacttt
      961 ttacaaatgc tgtcatggaa tttctggctt tagacgattt acaattacct tttattaaaa
     1021 caggccacct aattacaagt ttagatatgg ctggtttatc agttaccttg tgtcgtgtaa
     1081 aggatagtag atggattgat tacttaaaac ataagacaga tgctagagct tggtgataga
     1141 agtaagtggt aagagttttt agaatagaaa atactttaag gagaacagag aaatctgttc
     1201 tctttttgtt cgcttaaaga tcatagaaag gtgaagagaa catggaaggt attgaaataa
     1261 tattagatat agaaacgtta ggaatttttc aagtcataga tcatggtatc tctccagatg
     1321 atagagaaac atat
//
LOCUS       DATROQ010000176         1291 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.177, whole genome shotgun sequence.
ACCESSION   DATROQ010000176 DATROQ010000000
VERSION     DATROQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.177"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(209..574)
                     /gene="rplL"
                     /locus_tag="VBC94_002053"
     CDS             complement(209..574)
                     /gene="rplL"
                     /locus_tag="VBC94_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEO6980272.1"
                     /translation="MALNIENIIAEIKEATILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGEAAAAKDSFDVELTAAGDKKVGVIKVVREITGEGLKEAKAIVDNAPSVIKEGASEA
                     EANEIKEKLEAAGASVTLK"
     gene            complement(638..1138)
                     /locus_tag="VBC94_002054"
     CDS             complement(638..1138)
                     /locus_tag="VBC94_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEO6980273.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVEQDTNLRR
                     SLRESDVEFKVIKNSILTRAAEKAGLEDLKELFVGPSAVAFSNEDVIAPAKVISDFAK
                     DAEALEIKGGSVDGKFTSVEEINALAKLPNKEGMLSMLLSVLQAPVRNIAYAVKAVAE
                     KDEEVA"
     misc_feature    complement(1168..1290)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT          382 a          261 c          211 g          437 t
ORIGIN      
        1 tcacaaaact tcaattagaa aatcgtttaa ttattcaaga aatactttat aatgatattc
       61 taaatccata ttaaacaaaa cacccccaca aaatattgtg ggggtgtttg tggggaagat
      121 tcaattgaat ctttcctttt ttaatcgcct taactttaac gaaaagactt taaaactagg
      181 ctttcacctt taaacaacca atagatgatt atttaagagt aactgaagca cctgcagctt
      241 caagtttttc tttgatttcg ttagcttctg cttctgaagc accttctttg ataactgatg
      301 gtgcgttatc aacgatagct ttagcttctt taagaccttc gcctgtgatt tcacgaacaa
      361 ctttgataac gccaactttt ttgtctccag cagctgttaa ttcaacgtca aatgagtctt
      421 tagcagcagc agcttcacca ccagcagcgg cagcagctac aggagcagca gcagttacac
      481 caaattcttc ttcgatagct tttacaagat cgttaagttc aagaattgta gcttctttaa
      541 tttcagcaat aatattttca atgtttaatg ccattgttat ttcctccaaa aatatgtttt
      601 aatataaaat tatgtagcta ggctacttga agagtgctta tgccacttct tcgtcttttt
      661 ctgcaactgc tttgacagca tatgcaatgt tgcgtactgg cgcttgaagt actgaaagga
      721 gcattgaaag cataccttct ttgtttggta atttagcaag agcgttaatt tcttcaactg
      781 aagtgaattt accgtctact gaaccacctt tgatttcaag agcttctgca tctttagcga
      841 aatcgctaat aactttagct ggtgcaataa catcttcgtt agaaaacgca actgctgatg
      901 gaccaacaaa aagttcttta aggtcttcaa gacctgcttt ttcagctgca cgagttaaaa
      961 ttgagttttt gataacttta aactcaacat cgctttcacg aagtgaacga cgtaagttag
     1021 tatcttgctc aacagtaaga ccacgtgaat caacaacaac gatacttgct gctgctttca
     1081 ttttctcagc aataagttca acttgttcag ctttttttgc aataattgct tcactcatta
     1141 gttttacctc cgtttttatt ttttgggata ggcacaaaaa aatcgcacct aaacctagac
     1201 acgaaagtac aatacgttat tttataatga cgttttgtgc ctcggtagga atattatgag
     1261 cactgctccc ctactgtctt aggtcagttt c
//
LOCUS       DATROQ010000177         1280 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.178, whole genome shotgun sequence.
ACCESSION   DATROQ010000177 DATROQ010000000
VERSION     DATROQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.178"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..552
                     /locus_tag="VBC94_002055"
     CDS             <1..552
                     /locus_tag="VBC94_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEO6980274.1"
                     /translation="VIKYLQEHDIDSKIIPQIIDNLKSENWINDKNYVQSFIQQNLNN
                     GDKGPYVIKQKLLQKGIKSKIIESELKAINFQDLASKISQKLYKKYQNKLPLKALKDK
                     LMQSLTTKGFDYQIGHTVIQNLEIEKDQELEDDLIYKELDKQYQKLSKKHDQYELKQR
                     IINVLMRKGYQYEDIKSALREYL"
     gene            636..1169
                     /locus_tag="VBC94_002056"
     CDS             636..1169
                     /locus_tag="VBC94_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEO6980275.1"
                     /translation="MRLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTENDGRRWVTREPAIVYFHKKYWFNIIAMIRETGVSYYCNLASPYILDPEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEQHKAQMNYPTDIDYILKENVKILVEWINENKGPFS
                     SSYINIWYKRYLELKKR"
BASE COUNT          535 a          187 c          197 g          361 t
ORIGIN      
        1 gttattaaat atcttcaaga acatgatatt gattctaaaa ttattccaca aattattgat
       61 aatctaaaaa gtgagaattg gataaacgat aaaaactacg tgcaatcttt catacagcaa
      121 aatctaaata atggcgataa ggggccatac gtgataaaac aaaaattatt gcaaaaaggt
      181 atcaaaagta aaataattga atctgaactt aaagctataa actttcaaga cttagcatcc
      241 aaaataagcc aaaaactcta taaaaaatat caaaacaaac ttccactaaa agcattaaaa
      301 gataaattga tgcagagctt aactacaaaa ggatttgatt atcagatagg gcataccgta
      361 attcaaaacc ttgaaattga aaaagatcaa gaacttgaag atgacctcat ttataaagaa
      421 ttagataaac aataccaaaa attatcaaaa aaacatgacc agtatgaatt aaagcaacgt
      481 ataatcaatg tcttaatgcg taaaggatac cagtacgaag atatcaaaag tgctttaaga
      541 gaatatttat aagataataa ctctcaaacg atgtatttta cagaaaatta tgataaacta
      601 taacagacgt ataaattgta gaaagttggt aggctatgag attacctaaa gaaggcgact
      661 ttattacaat tcaaagttac aaacatgatg gtagtttaca ccgaacttgg cgtgacacca
      721 tggtattaaa aacaaccgaa aatgccctca ttggtgttaa tgatcatact ttagtaacag
      781 aaaatgatgg tcgacgctgg gtgacacgag agcctgcaat agtatacttt cataaaaaat
      841 attggtttaa cattatcgct atgatacgtg aaactggtgt ctcctactat tgtaatctag
      901 caagtccgta tatcttggat cctgaagcac tcaagtacat cgactacgac cttgatgtca
      961 aagtatttgc agatggtgaa aaaagactac tggatgtgga cgaatatgaa cagcataaag
     1021 ctcagatgaa ctatcctacc gatattgatt atatattaaa ggaaaatgta aaaatattgg
     1081 tagaatggat aaatgagaat aaaggcccct tttcatcatc atatatcaat atctggtata
     1141 aacggtacct tgaattgaaa aagcgctaac taacacacta aatagtgtgt ttttattaat
     1201 atcaaattta attacaatac tattgcaaaa atatatactt aaaataaaaa aagtagaaag
     1261 tgatctttct acttttttaa
//
LOCUS       DATROQ010000178         1272 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.179, whole genome shotgun sequence.
ACCESSION   DATROQ010000178 DATROQ010000000
VERSION     DATROQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.179"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            52..>1272
                     /locus_tag="VBC94_002057"
     CDS             52..>1272
                     /locus_tag="VBC94_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6980276.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMATLSIISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTNSIDSYVKRINSVADLV
                     DHDIIETQDTLANQSPERAKNFKRTVMLTGVNDSAKETKFVSEAYKLVEGKHLENKDK
                     NKILMHKDLAKKNNLKVGDKIKIKSNLFDADNEKVANETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHSAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFVGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFIIEMVFISIPALLGSYFLAQYTADKLGNNILNKVTGDIAKQIARQ
                     SASSQLGGGAEAEGF"
BASE COUNT          516 a          157 c          227 g          372 t
ORIGIN      
        1 aagtggtaaa aagttgaaaa aagtaaataa aatgaatttg gaggttgaat aatgataaaa
       61 aatgcatttg cttatgtaac tagaaaaagc ttaaaatcat taattattat attagttatt
      121 ctatcaatgg caactttaag catcattagt ttatctatta aagatgccac agatagagct
      181 tctaaggaaa catttgctaa tataacaaac agtttttcta tggaaataaa tagacaagta
      241 aatccaggaa cacctagggg tggaggaaat gtaaaaggtg aagatattaa aaagatatct
      301 caaacaaaca gtatagactc ctatgtaaaa agaataaaca gcgtcgcaga cctagttgat
      361 catgatataa ttgaaactca agatacactt gcaaatcaat cacctgaaag agcaaagaat
      421 tttaagagaa cagttatgtt aacaggggtt aatgactcag caaaagaaac aaaatttgta
      481 tcagaagcat ataagttagt agaaggaaag catttagaaa ataaagataa aaataaaatt
      541 ttaatgcata aagatttggc aaaaaaaaac aatctcaaag ttggagataa aataaaaata
      601 aaatctaatt tgtttgatgc tgacaatgaa aaagttgcaa atgaaactgt agaagtagaa
      661 attaaaggtt tatttgatgg tcataatagc ggtggggtaa gtgcagcaca agaactatac
      721 gaaaatacac taatcactga cgttcattca gctgctaaag tttatggtaa tacagaagat
      781 acagccgtat accaagatgc aacattcttt gtaaaaggtg ataagaatct tgatagtgta
      841 ataaaagatc ttgggaaact agatataaat tggagagaat ataatttgat taaaagttca
      901 tcaaattatc ctgcgctgca acaatctata tccggtatct attctatttc aaacaaatta
      961 tttgttggct cattaatatt tgcaggagtt gtagtttcat tattactttt cttatggatg
     1021 aatgccagaa agaaagaaat agcagtatta ctatcattag gtatatctaa attagaaatt
     1081 tttggtcaat ttataatcga gatggtattt atatccattc ccgcacttct tggatcttat
     1141 ttcttagctc aatatacagc tgataagcta gggaataata tattgaataa agttactggc
     1201 gatatagcta aacaaatagc tagacaatct gcatctagtc aattaggcgg gggggcagaa
     1261 gctgaaggat tc
//
LOCUS       DATROQ010000179         1211 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.180, whole genome shotgun sequence.
ACCESSION   DATROQ010000179 DATROQ010000000
VERSION     DATROQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.180"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(31..1167)
                     /gene="dnaN"
                     /locus_tag="VBC94_002058"
     CDS             complement(31..1167)
                     /gene="dnaN"
                     /locus_tag="VBC94_002058"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEO6980277.1"
                     /translation="MIHFSINKNFFLHALTVTKRAISHKNAIPILSTVKIEVTRDAII
                     LTGSNGQISIENTIPASNENAGLLVTNPGSILLEAGFFINIISSLPDVTLEFTEIEQH
                     QIVLTSGKSEITLKGKDVDQYPRLQEMTTDTPLTLETKLLKSIINETAFAASQQESRP
                     ILTGVHLVISQNKYFKAVATDSHRMSQRTFQLEKSANNFDLVVPSKSLREFSAVFTDD
                     IETVEVFFSDSQMLFRSENISFYTRLLEGNYPDTDRLLTNQFETEIIFNTNALRHAME
                     RAYLISNATQNGTVRLEIQNETVSAHVNSPEVGKVNEELDTVSLKGDSLNISFNPTYL
                     IESLKAVKSETVTIRFISPVRPFTLTPGEDTEDFIQLITPVRTN"
BASE COUNT          398 a          190 c          208 g          415 t
ORIGIN      
        1 acccaagaat aacttgggta gttttcttat ttagttagta cgaacaggag ttattaattg
       61 gatgaaatct tcagtatctt caccaggggt caaagtaaat ggacgtactg gagaaataaa
      121 tcgaatcgta actgtttcgc tttttactgc ttttaaagat tcaattaggt aagttggatt
      181 aaaactaata tttaaactat cacctttaag gctaacagta tccaattcct cattaacttt
      241 accaacttct ggagagttta catgagctga gactgtttca ttttgaattt ctaaacgaac
      301 agtaccgttc tgagttgcat tcgaaattaa ataagcacgt tccatagcat ggcgtaaagc
      361 atttgtatta aagataattt cggtttcaaa ctgattagtt aggaggcgat cagtatcagg
      421 gtagtttcct tcaagcaaac gtgtatagaa gctgatattt tcacttctaa ataacatttg
      481 actatctgag aagaaaacct ctacagtttc aatatcatct gtaaaaacag ccgaaaattc
      541 tcgaagggat ttacttggaa caaccaaatc aaaattatta gccgatttct ctaattggaa
      601 agtgcgttga ctcatacggt gtgaatctgt cgcaacagcc ttaaagtatt tattttgact
      661 gataaccaaa tgaacacctg ttaagattgg acggctttct tgttggctag cagcaaaagc
      721 agtttcatta ataattgatt ttaacagttt agtttctaat gttaatggag tatctgttgt
      781 catttcctgc agacgagggt attgatcgac atccttacct ttcaaagtaa tctctgattt
      841 tccactagta agaacaattt gatgttgttc aatctctgta aattctaaag ttacatctgg
      901 taaacttgaa ataatattaa taaagaaacc agcttctaac aaaatagagc ctggattcgt
      961 tactagtaaa cctgcatttt catttgaagc aggaatagta ttttcaattg aaatttgtcc
     1021 atttgacccc gttaaaatga tagcatctct agtcacttct attttaacag ttgaaaggat
     1081 tggaatcgca tttttatgac taatagctcg tttggttact gttagagcat gcaagaagaa
     1141 atttttattt attgaaaaat gaatcatagt atatccttta tatattagta taaatttagt
     1201 agtaatagta g
//
LOCUS       DATROQ010000180         1182 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.181, whole genome shotgun sequence.
ACCESSION   DATROQ010000180 DATROQ010000000
VERSION     DATROQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.181"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            160..1065
                     /locus_tag="VBC94_002059"
     CDS             160..1065
                     /locus_tag="VBC94_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6980278.1"
                     /translation="MKNQQKLMYLESIELYSNITKAAAHLFISQPYLSKVIKQLENEL
                     EIKLIQSQGHQTFLTYAGQRYLFYLKEIDMIERQMAKELYLIRSDKKGEITLGINSGL
                     ASSILANVLPKFNLEHPEISVKLLENNQNISEQLVASGDIDLAVGMAPILYKDGIAST
                     TIYRDELFLMIPTTSQLYNAEKRGQIIPFEYPISVLDNEPLILTPLEYGIGKTIAQFY
                     ELHHMSLNQMITTSTVPTAASLSLSGMGATFVPQTLIHRYLDKECNVYHFHKNKLFSE
                     YIMIYKKDVELSGIALLLYKAFLTK"
BASE COUNT          412 a          218 c          167 g          385 t
ORIGIN      
        1 atttttttta gatatttcta taactaaata gttatgaata tatcatgtta tactactaaa
       61 ttaaatagat tgattttagc tcaaaatgat agtaaactta tagccataac gtatcataac
      121 ttattattaa tctaaggata tcaaactggt tataaagtct tgaaaaatca acaaaaatta
      181 atgtatctag agagtatcga gctatactca aacatcacta aagccgctgc acaccttttt
      241 atttctcaac cttacctaag caaagtgatc aagcaattag aaaatgagtt agaaataaaa
      301 ctcatccaga gccaaggaca ccaaaccttt ttaacttatg ctggacagag atatctattc
      361 tacctaaaag aaattgatat gattgagcgt caaatggcca aagaactata tctcatccgt
      421 tctgataaaa aaggagaaat cacacttggg attaactctg gcttagcaag ttctattctg
      481 gcaaatgtac ttcccaaatt taacttggag cacccagaaa tttctgtcaa attactcgaa
      541 aacaatcaaa atatttctga acaattggta gcgagtggtg atattgacct agctgttggg
      601 atggctccta tcctttataa agatggaatt gcatcaacca ctatttaccg cgacgaattg
      661 tttttaatga tacctaccac cagccaactc tacaacgctg aaaaacgtgg acagattatt
      721 ccttttgaat atcctatttc agttctagac aatgaacctc ttatcctaac accattagag
      781 tacggaattg gcaaaacaat tgcacaattc tatgagcttc atcacatgtc attaaatcag
      841 atgattacaa ctagtaccgt tcctacagca gctagcctat ctttgtcagg gatgggagca
      901 acatttgttc cacaaactct cattcatcgc tatttggata aagagtgcaa tgtctaccat
      961 tttcataaga ataagttatt ttcagagtac attatgattt ataaaaaaga tgttgaatta
     1021 tctggaattg ctctcctact ttataaagct tttttaacca agtagtttcg ctaattctat
     1081 tgaaaatcag tttacttctt accaaaatcg gtataaaata gacctatgat atacaaaatt
     1141 attgcttctt tatttcttgt tttaatccct atattttcac ag
//
LOCUS       DATROQ010000181         1179 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.182, whole genome shotgun sequence.
ACCESSION   DATROQ010000181 DATROQ010000000
VERSION     DATROQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.182"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..730
                     /locus_tag="VBC94_002060"
     CDS             <1..730
                     /locus_tag="VBC94_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6980279.1"
                     /translation="STVSRSDVNIIMTVNTNTHKVLLTTTPRDAYVKIPDGGGNQYDK
                     LTHAGLYGVETSMKTLENLYDINLDYYARINFSSFLKLIDLLGGVTVYNDQAFTSKHG
                     NFDFPVGQVTLNSEQALGFVRERYSLQGGDNDRGRNQEKVIAAIINKLASSQSVTKLN
                     SITSQLQTSVQTNMTIDNINDLINNQLSTGQRFTVESQALTGHGSTGELPSYAMPGAQ
                     LYMMSIDQSSLSNAKSKIKNTMEE"
     gene            736..>1179
                     /locus_tag="VBC94_002061"
     CDS             736..>1179
                     /locus_tag="VBC94_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEO6980280.1"
                     /translation="MIDIHSHIVFDVDDGPKTLEESLSLIEESYRQGVRIIVSTSHRR
                     KGMFETPEDIIFKNFSIVKHEAEKRFEHLQILYGGELYYTSDMLEKLKLKQIPTLNNT
                     KFALIEFSMQTSWKDIHTALSNVLMLGITPVVAHIERYNALENQKE"
BASE COUNT          430 a          183 c          206 g          360 t
ORIGIN      
        1 atcaacagta tcaagatctg atgtaaatat tattatgacg gttaatacca atacccacaa
       61 agtattgtta acgacaacac cacgagatgc ctatgtaaaa attccagatg gtgggggcaa
      121 tcaatatgat aaattaaccc atgcaggttt gtatggtgtt gagacatcaa tgaaaacact
      181 tgaaaacctt tacgacatca accttgatta ttatgctaga attaattttt catcattttt
      241 aaaattaata gacctcttgg gaggagtgac agtttataac gatcaagctt ttacaagtaa
      301 acatggtaat tttgactttc ctgttggtca agtaacattg aattctgagc aggctttggg
      361 ctttgttaga gaacgttatt ctctacaagg aggcgataac gatagaggta gaaatcaaga
      421 aaaagtgatt gcagctatta taaataagtt agcttctagt cagtcagtaa caaaattaaa
      481 tagcattacc tcacagctcc aaacgtccgt tcaaactaat atgactattg ataatattaa
      541 tgatttgatt aacaatcaat tgtcaactgg acaacgcttc actgtcgagt cacaagcatt
      601 aactggtcat ggttcaacgg gtgaactccc ttcatatgca atgccaggag ctcaacttta
      661 tatgatgtca attgatcaat ctagcttatc taatgcaaaa tcaaaaatta agaacacaat
      721 ggaggaataa caagaatgat tgatattcat tctcatatcg tttttgatgt agatgatggg
      781 cccaaaacgc ttgaagagag tttatcttta atagaagaaa gttatcgaca aggtgttaga
      841 ataattgttt cgacatcaca tagaagaaaa ggtatgtttg aaacacctga agatattatc
      901 ttcaaaaact tttctatagt taaacacgaa gcggaaaaaa gatttgaaca tcttcaaatc
      961 ttatatggtg gagaattata ctatacaagt gatatgttag aaaaactgaa gttaaagcaa
     1021 attccaactt taaacaatac gaaatttgct ttaattgaat tttctatgca aacttcttgg
     1081 aaagatattc atacagcttt gtcaaatgtt ttaatgcttg gtattacacc agtcgttgcg
     1141 catatagaga ggtataacgc tttagagaat caaaaagaa
//
LOCUS       DATROQ010000182         1175 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.183, whole genome shotgun sequence.
ACCESSION   DATROQ010000182 DATROQ010000000
VERSION     DATROQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.183"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            13..>1175
                     /locus_tag="VBC94_002062"
     CDS             13..>1175
                     /locus_tag="VBC94_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO6980281.1"
                     /translation="MIDFLLLKSLHSLLGLTITVCDQNFSVIREYKSEKTISLFYNHY
                     LILSNFSKTQHDFLFHYGSLGELFLVHHIQQYYIIIGPWRSNVIDPLLLKKKLTETQI
                     NASEQDYFIDRLSQLPFFSLSQIRELLIVTNYCLTGVVKDKLSEPLHYYTKGWSNSFD
                     LDKIKQFSKQNMSSYKYQYHFENNILKAVKSGSEFLLKETVEQFSNSIVPIISGDELR
                     SEKNYSIMIYDRLSQATIQAGLDIETAYRARDRFIKENESTISLNEVLKLRDTAILFY
                     TQQVHSLKKHLETPHSQTIVAVIRYLENNLNRFIKTEEIAKECHMSESKLRKLFKQEK
                     HITIQQYFLNLKIEAAKQLLDENKKVEEVSNLLGFSTSSNFSRTFKKIVGISPL"
BASE COUNT          424 a          203 c          163 g          385 t
ORIGIN      
        1 gtggtaactc atatgattga ttttctactg ttgaaatcat tacacagtct tctaggtcta
       61 actattactg tttgtgatca aaatttttct gttatcagag agtataaatc cgagaaaact
      121 atttcacttt tttacaatca ttaccttatt ttaagtaact ttagtaaaac tcaacatgat
      181 tttttatttc attatggctc tttaggagaa ctttttcttg tccaccatat tcaacaatat
      241 tatattatca tcggtccctg gcgtagcaat gttattgacc ctttacttct taagaaaaag
      301 ctgactgaaa cacaaatcaa tgctagtgaa caagactact ttattgatag actatcccaa
      361 ttacctttct tctcacttag tcaaatccga gaattactaa tagtaaccaa ttattgtcta
      421 actggggttg tgaaagataa attatcagag cccttacatt actacacgaa agggtggagc
      481 aactcctttg atctagataa aattaagcaa ttttctaaac agaatatgag ttcttacaaa
      541 tatcagtatc attttgaaaa taatattcta aaagctgtta aatcaggtag tgaatttctt
      601 ttgaaagaaa cagtggaaca atttagcaat tctatcgtac ctataatcag tggagatgaa
      661 ttgcgatctg aaaaaaacta ctccatcatg atttatgatc gtctctcaca ggctaccatc
      721 caagcaggac ttgatatcga aacagcttat cgagcacgag atcgttttat aaaagaaaac
      781 gagtcaacta taagtctaaa tgaagtttta aaattacgtg atactgctat cttattctat
      841 actcaacaag ttcattcttt aaaaaaacat ctcgaaaccc ctcattctca aactattgtc
      901 gcagtgattc gatatcttga aaataatcta aatcgcttta ttaaaacaga agaaatagct
      961 aaagaatgtc acatgagtga atcgaagtta cgaaaactat ttaagcaaga aaaacacatc
     1021 actattcaac aatattttct aaacttaaaa atcgaagctg ctaagcaatt actagatgaa
     1081 aataagaaag tagaagaagt ttccaattta cttggatttt ccacttcttc taatttttca
     1141 aggacattta aaaaaatagt gggaattagc ccact
//
LOCUS       DATROQ010000183         1141 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.184, whole genome shotgun sequence.
ACCESSION   DATROQ010000183 DATROQ010000000
VERSION     DATROQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.184"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(468..608)
                     /locus_tag="VBC94_002063"
     CDS             complement(468..608)
                     /locus_tag="VBC94_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980282.1"
                     /translation="MLLIDVMIHLSEENEKGFTKLQFFTRQIVVGVNWYVLPKIDNYF
                     NP"
     gene            complement(667..966)
                     /locus_tag="VBC94_002064"
     CDS             complement(667..966)
                     /locus_tag="VBC94_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HEO6980283.1"
                     /translation="MLLPVGSVVYLIDGNQKLVIVNRGAIVEQEGQEVYFDYLGGIFP
                     EGLNLEQVYYFNQEDIDEVVFEGYHDEEEERVSRLIEKWKNTEGKNLPKGKTSVE"
BASE COUNT          366 a          196 c          172 g          407 t
ORIGIN      
        1 aaaggtagta aactatagtt gtcttattga aacagataaa ataaatcacc tacggagcta
       61 ttcgtatgaa aatgtagtta ttctcgatat aagttttaga tacgaactta atttgctcta
      121 ttgaagctgg taaaggaaat cggggtggtg ttagtggacg tgcgctaagc attggtactg
      181 agcttggtga taccaatctt gcttactaca tcaggtagct ctctgtttta gtgccatatg
      241 caattggaat aagagctgtc attggtattg aaggagccct agtccactga tcattgaatt
      301 actaatttag gaggtggaat gatgaatttt aatatgtctc attatagaaa gttacaacta
      361 tgtcgttttc taatcgtaca gatacctgcg ctggctttat tagctattct tttatacggg
      421 attttcctaa agtacagtct attttcaaaa gtattttttc ttatctttta ggggttgaaa
      481 tagttatcaa ttttgggaag tacataccag tttacaccaa ccacaatttg tcgagtaaag
      541 aattgtaatt tagtaaagcc tttttcgttt tcttctgata agtgtatcat gacatctatc
      601 aatagcatat tcacactagt agcatattga tttgtttttg agtagaattt actttttaga
      661 ttatttctat tctacactag tctttccctt tggaagattc tttccctctg tatttttcca
      721 tttttcaatg agacgagaaa ctctttcttc ctcctcatca tggtaacctt caaaaacaac
      781 ctcatcaata tcttcttgat taaaatagta aacttgttca agatttaaac cctcaggaaa
      841 aattccacct aggtaatcaa aataaacttc ttgtccttct tgctcaacaa ttgcacctct
      901 atttacaata actaattttt gattgccatc aataagatag acaacactcc ctactggtaa
      961 taacatactt cagttctccc caatataata atttttccca tttaacgatg gttgaaactc
     1021 aatgaacttt ataaaaaata aactgtttga aaaaataaat ccaaataaaa ataagtttga
     1081 aaagttttcg tcccacaatg ccattcccca tagaacgata taagaaatta tgattgataa
     1141 t
//
LOCUS       DATROQ010000184         1139 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.185, whole genome shotgun sequence.
ACCESSION   DATROQ010000184 DATROQ010000000
VERSION     DATROQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.185"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            304..>1139
                     /locus_tag="VBC94_002065"
     CDS             304..>1139
                     /locus_tag="VBC94_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEO6980284.1"
                     /translation="MKICILGLGVIGTTYGYIFQKAGHQVEHLVRESKKQDVPLKLDI
                     TMLDGRYNNKGEEKTDIYTVNLAKPNTNYDFILLSVAGGRIKDAIATLSQNNITGSLL
                     LFCNFWNSREEIEKMVGDFKYIIGFPTAGGNLKQKKLNCVLFDHIMLESEEKATITNY
                     KELLEFLESADIKVENPYDMIEWIWIHMAINAGVTSTAARAGKIDDPKQLAVNLMRDN
                     HGLSIAVKSIRETIKVVKNRGVDLRLYKNELFPYKIPSTIAGIIMKRMFARNELTSRI
                     MT"
BASE COUNT          461 a          133 c          220 g          325 t
ORIGIN      
        1 atgtttattt gaatgatgat attatctttc agttagctga aatctcagaa tttttaaagt
       61 caagtcagtc aacatttaaa caggagcgta gtccatattc tcaaataata gacctacgaa
      121 aagaagaagc acaagctaaa agtgttagga atagcataga aaaattattg taagaaaaca
      181 gtgatctaat acaggtaagg aagtatgagt taggctataa aactaagtaa aaaaatatga
      241 tataatataa aaaattatcg agtaaaataa actggaagcg aaacttaagg agatgattat
      301 taaatgaaaa tatgtatttt aggattaggc gtaataggca caacttatgg atatatcttt
      361 caaaaagcag gacatcaggt tgaacattta gttagagaaa gtaaaaagca agatgttcca
      421 ttaaagctgg atataaccat gttagatggg cgatataaca ataagggaga agaaaaaaca
      481 gatatatata ctgtgaatct tgcaaaacca aatacaaatt atgactttat ccttttaagt
      541 gttgcaggcg ggaggataaa agatgccata gcaacacttt cacaaaataa tattacagga
      601 agtttgttat tattttgtaa cttttggaac agccgtgaag aaatcgaaaa aatggttggt
      661 gattttaaat atatcattgg atttccgaca gcaggtggga atttgaagca gaaaaagttg
      721 aattgtgttc tgtttgacca tataatgctt gaaagtgaag aaaaagcaac tatcacaaac
      781 tataaagagt tgttagaatt tctcgaatct gccgacataa aagtagaaaa tccttatgat
      841 atgatagagt ggatatggat tcatatggca ataaatgcag gtgttacttc tactgcagca
      901 agggcaggaa aaattgatga tccaaaacaa cttgcggtta atcttatgag agataatcat
      961 ggattgtcta tcgcggtaaa atcaataaga gaaacaatta aagtagtaaa aaatagaggg
     1021 gtcgatttaa gattatataa aaatgaactt tttccttata aaataccttc tactattgca
     1081 ggaataatta tgaaacgaat gttcgcaaga aatgaactta cgagtagaat tatgacgtt
//
LOCUS       DATROQ010000185         1134 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.186, whole genome shotgun sequence.
ACCESSION   DATROQ010000185 DATROQ010000000
VERSION     DATROQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.186"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..322)
                     /locus_tag="VBC94_002066"
     CDS             complement(<1..322)
                     /locus_tag="VBC94_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEO6980285.1"
                     /translation="MKNFIKQSDTTFVRIIKSLLIGGFIGAILGSVGALFNIFGQDKY
                     LSEINIVQYFLWVSRIVVIITALFSLIYLYQIQKYQKVFFNVDESQSEEIYRQINLRH
                     SYGMT"
     gene            complement(325..540)
                     /locus_tag="VBC94_002067"
     CDS             complement(325..540)
                     /locus_tag="VBC94_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6980286.1"
                     /translation="MGLILKNRLKELRARDGINQTELAKLTGVSRQTISLIERGEYTP
                     SVIIAMKISQVFKESLENVFSLVSTEE"
     gene            complement(585..878)
                     /locus_tag="VBC94_002068"
     CDS             complement(585..878)
                     /locus_tag="VBC94_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980287.1"
                     /translation="MVMTYIGVPKEQKALSRRGKQLEKINQGGKTLMSQMIQDLVGST
                     CMIYLEDGFQTHPYEILKVDEDWVQIRRNLKRSKEEVRILRIHDIKELRPVNK"
     gene            complement(910..1098)
                     /locus_tag="VBC94_002069"
     CDS             complement(910..1098)
                     /locus_tag="VBC94_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980288.1"
                     /translation="MKKEFIITGRMVARVMETLVCLCMVSNLVLDMLDIIEINEIVFC
                     VLVASIAGLEFCLQKERE"
BASE COUNT          371 a          233 c          140 g          390 t
ORIGIN      
        1 aagtcatccc ataagaatgt cttaaattaa tctggcgata aatttcttca gattgacttt
       61 catcaacatt gaaaaaaact ttctgatatt tttgaatctg atagaggtag atgagactaa
      121 agagagctgt tataattaca acaatacgac taacccatag gaaatattga acaatattaa
      181 tttcagataa gtacttatct tgaccaaata tgttaaaaag agctcctacg gaaccaagta
      241 tggcccctat aaaaccacca attaaaagac ttttgataat tcgaacaaat gtagtatcac
      301 tttgttttat aaaatttttc atcgttattc ctccgttgac actaaactaa aaacattctc
      361 tagggattct ttaaatactt gagaaatctt catagcaata ataacagatg gcgtatattc
      421 tccgcgttca attaggctaa ttgtttgtct tgagactccc gttaatttgg cgagttctgt
      481 ttgattgata ccatctcgtg cacgcaattc tttgagcctg ttttttaaaa ttaatcccat
      541 catttttctc ctttataaaa tgatatatag atttattcct cactctattt attgactgga
      601 cgcaattcct tgatatcatg aatacggaga atacgtacct cctctttact tcgtttcaag
      661 ttgcgacgaa tctgtaccca atcctcatct acctttaaaa tctcataagg atgcgtttga
      721 aaaccatcct ctaaataaat catacatgtg cttcctacta aatcttgaat catttgtgac
      781 ataagtgttt ttcctccctg atttattttt tctaattgtt tacccctacg actgagtgcc
      841 ttttgctctt taggtacacc aatatacgtc atgaccataa aaagccatta agccaaaaat
      901 tccatctatc tactcccttt ctttttgcaa acaaaactca agccctgcaa ttgatgctac
      961 caacacacaa aatacaattt catttatttc tattatatca agcatatcaa gaacaagatt
     1021 acttaccata cataagcaaa ctagggtttc cataactctt gcaaccatcc gacctgtaat
     1081 aataaattcc tttttcattt gagtaactcc taaaaccatt atgcttttga aaca
//
LOCUS       DATROQ010000186         1107 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.187, whole genome shotgun sequence.
ACCESSION   DATROQ010000186 DATROQ010000000
VERSION     DATROQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.187"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..228
                     /locus_tag="VBC94_002070"
     CDS             <1..228
                     /locus_tag="VBC94_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980289.1"
                     /translation="PETELKGVSYKIPINSEIVAPFIKQLNIKNPKKSSISVTKTESK
                     EYYYTISIDTDSEIYSIFEGITLEDVKEFSK"
     gene            225..755
                     /locus_tag="VBC94_002071"
     CDS             225..755
                     /locus_tag="VBC94_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980290.1"
                     /translation="MSPSEKLDRRIDSSSDLSQYVYDFEKQYTEYMKKGYSEKNASIQ
                     ARRTLNEILQENNPGLRSSDAMYDKETGIAAIAVKDKNTGETYIAYAGTNKDADGATD
                     FISDANIGANNSAYLKRLGDKATSFYDRVQSNGDNITVTTGHSYGDFLASRVAIELIP
                     FFINFRKVNDSFHFCY"
     gene            847..1070
                     /locus_tag="VBC94_002072"
                     /pseudo
     CDS             847..1070
                     /locus_tag="VBC94_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          405 a          161 c          198 g          343 t
ORIGIN      
        1 cccgaaactg aattaaaagg tgtttcctat aagattccta taaattctga aattgtagcc
       61 ccttttataa agcaattaaa tataaaaaat cctaaaaaat catctatttc ggttacaaaa
      121 acggaaagta aagaatatta ttatacaatc agtattgata ctgattctga gatatattct
      181 atattcgaag gtattacatt agaggatgta aaggagtttt ctaaatgagt ccaagtgaga
      241 aattagatag aagaattgat tcttcatcag acctctccca gtatgtatat gattttgaaa
      301 agcaatatac agaatatatg aagaaggggt attctgaaaa aaatgcgagt atacaagcac
      361 gacgaaccct gaatgaaata ctacaagaaa ataatccagg attgagatct agcgacgcca
      421 tgtatgataa agaaacaggt attgcagcta ttgctgtaaa agataaaaat actggtgaga
      481 cttatatagc ttatgcgggt actaataagg atgctgatgg ggcaaccgac tttatttcag
      541 atgcaaatat cggtgctaat aactctgctt atttaaaacg attaggagac aaagctacta
      601 gcttttatga tcgtgttcaa agtaatggcg ataatattac tgtcacaaca ggtcattctt
      661 atggtgactt tctcgcttct agagtggcaa ttgagctaat tccattcttt attaacttta
      721 gaaaagttaa tgattcattt cacttttgtt attagcttgt gatcacttat aaagtgacag
      781 ctagttcatg aaatttctta ggagttttct ggctttaaag gtaacctatg atttaaaaaa
      841 catctcatgg aattcaatgc ggaatcctca acagtctttt gtggactaac agaagttcag
      901 gatgtcgttt cggcaggcct taactattct cagaaggttt tggaaatttc aatggtacat
      961 ttgatacctc aggtaaggga ttagaatgga caaaaacaat tcaaacaggt atgaaaaaac
     1021 gagaagagaa aatcaaatac cctcatttta caattgttga cataaagtaa ttaaacaaaa
     1081 tccggaaaat ttatattaag taatttt
//
LOCUS       DATROQ010000187         1076 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.189, whole genome shotgun sequence.
ACCESSION   DATROQ010000187 DATROQ010000000
VERSION     DATROQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.189"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(1..425)
                     /locus_tag="VBC94_002073"
                     /pseudo
     CDS             complement(1..425)
                     /locus_tag="VBC94_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            603..803
                     /locus_tag="VBC94_002074"
     CDS             603..803
                     /locus_tag="VBC94_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6980291.1"
                     /translation="MSQEKLKSKLDQAKGGAKEGFGKITGDKELEAKGFIEKTIAKGK
                     ELADDAKDAVEGAVDAVKEKLK"
     gene            845..1033
                     /locus_tag="VBC94_002075"
     CDS             845..1033
                     /locus_tag="VBC94_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO6980292.1"
                     /translation="MSIRTDIDEFKEAIDKGYISGNTVAIVRKNGKIFDYVLLHEEVR
                     EEEVVTVERVLDVLRKLS"
BASE COUNT          381 a          150 c          214 g          331 t
ORIGIN      
        1 aaagaatgca tgatattgtt gtcatcaatt atttttctaa attccctggc cttaaattgt
       61 gacccttggt cggaatgaaa gagaagtgtt ccttcaatct ttcttttatt aagagctaat
      121 tctagagtgt cacatgctaa ctttgcatcc atacgatgac tcaatttcca agcaatatat
      181 tttctagaat agagatcaat tattgcgcag agatagacat atttcttata tccaatagaa
      241 atataggtga agtcagtaga ccatacttga ttaggtttat caggattaaa tttctggttg
      301 agtaagtttt gaggatacgt tttttgagtc tttttaagag ctgtttttgt cttaacggta
      361 gccattttag ggagcgccat attcttcatc agacggtaaa ttcttccttc agagattttt
      421 gtgtctttta aaacaggtta taacacacaa agtgttgtct atttttatag tatacatcca
      481 tactcgcata accatgagat aatcactaaa acaagagtcg cctgacacta gcgactcttg
      541 tttttatttg ctaaagaaag atatagatgt tatgattaat aaaaataata aggaggtgca
      601 gtatgtcaca agaaaaacta aaatcaaaat tagatcaagc aaaaggtggt gctaaagaag
      661 gctttggcaa aataaccggt gataaagagt tagaagcaaa aggatttatc gaaaaaacaa
      721 ttgctaaagg caaagaacta gcagatgatg ctaaagatgc tgttgaaggg gcagtagatg
      781 ctgtcaaaga aaaactgaag taacaaataa ccgctcattt aacgagcggt tatttgttat
      841 ctttttgtct atcagaacag atatagatga gtttaaagaa gcgattgata aaggctatat
      901 ttcagggaac acagtagcga tagtgcgtaa aaacggaaag atatttgatt atgtgttact
      961 acacgaagaa gtgagagaag aagaggttgt tacagttgag agagtgcttg atgtactgag
     1021 gaagttatca taataacgga ccaaaattcg ccccaaaaag tattttaaaa tagaat
//
LOCUS       DATROQ010000188         1054 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.190, whole genome shotgun sequence.
ACCESSION   DATROQ010000188 DATROQ010000000
VERSION     DATROQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.190"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            138..449
                     /locus_tag="VBC94_002076"
     CDS             138..449
                     /locus_tag="VBC94_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEO6980293.1"
                     /translation="MIPSQMLKGLLEGAILKIIQQEETYAYEISKKLNSYGFGEISEG
                     TIYPIILRLQSNKYIKGQQKPSPQGPKRKYYTLTNEGVEQLAQFKQNWESLSKALNKL
                     L"
     gene            470..>1054
                     /locus_tag="VBC94_002077"
     CDS             470..>1054
                     /locus_tag="VBC94_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980294.1"
                     /translation="MDKLKKLQKENTLLEGRIDNSNNQTYTDMIVYLRGASISPYHQE
                     LIRNDIVNMLLEAQERQASLVSVFGEDRHDFINQVIKSTPKISKKEETLQRWDLAILL
                     LTIQMIIFLGGYLITEALQQSVPDLIPITLLDVLFAIFISIIAVKITDTIIYATYNFD
                     KSKEKKYFFRYMFLILSLIIAYILIGKYCHLPFIN"
BASE COUNT          394 a          181 c          155 g          324 t
ORIGIN      
        1 caaacctatt ttagaagaat catcgtctat cactcttatt ttctaccggt aacttagtta
       61 tatttgacat ctcaactact ttgtattaca atgtagttaa ccagtaactt gtattacaat
      121 atagataagg atataatatg attccatcgc aaatgctaaa aggattactt gaaggagcta
      181 tcctaaaaat catacagcaa gaggagactt atgcttatga aatctcaaaa aaattaaatt
      241 cttatggttt tggggaaatt tccgaaggga caatctaccc cattatctta aggctacaaa
      301 gcaataaata tataaaagga caacaaaagc cttcccctca aggacctaaa cgtaaatatt
      361 atacgttaac caatgaaggc gtggagcaat tagcccaatt taaacagaat tgggagtcac
      421 ttagtaaagc attaaacaaa ctactctaaa aattggagag gaaactagca tggataaact
      481 gaaaaaatta caaaaagaaa atacgctatt agaaggaaga attgataact ctaataacca
      541 aacctataca gatatgattg tctatttgcg aggtgctagc attagcccct accaccagga
      601 gctcattcga aatgatattg tcaatatgtt attagaagca caagagcgac aagcaagttt
      661 agttagtgta tttggagaag ataggcacga ctttataaac caagttatta aatctacacc
      721 taaaatctct aagaaagaag aaacattaca acgttgggat ttagccattt tactactaac
      781 tatccaaatg attatctttt taggaggata tctcattact gaagcacttc aacagtcagt
      841 accagatcta atccctatta ctctattaga tgttcttttt gcaatcttta tcagtattat
      901 tgctgtcaag ataactgata ccataatata tgctacatat aacttcgata aatcgaaaga
      961 aaagaaatat ttctttcgat atatgtttct aattctttcg cttatcatag cttatatcct
     1021 tataggaaaa tattgtcatc ttcctttcat taat
//
LOCUS       DATROQ010000189         1045 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.191, whole genome shotgun sequence.
ACCESSION   DATROQ010000189 DATROQ010000000
VERSION     DATROQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.191"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..127)
                     /locus_tag="VBC94_002078"
     CDS             complement(<1..127)
                     /locus_tag="VBC94_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6980295.1"
                     /translation="MAISIKSINLQNFRGFEDLHLNLNKYVTCISGHNGTGKSTIL"
     gene            complement(252..638)
                     /locus_tag="VBC94_002079"
     CDS             complement(252..638)
                     /locus_tag="VBC94_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO6980296.1"
                     /translation="MTIEEILDFHNVELAYFDNDLWPRPGIYVDEIKVVFVNKALSDE
                     SKKKVIFHELGHIDHDSNQYGRRHEEFELEANRFMIRCLLEDEFDEVEDKHEFNYLSF
                     MKRHNLKTTTDEVMVIDEYYNLLDAV"
     gene            complement(642..989)
                     /locus_tag="VBC94_002080"
     CDS             complement(642..989)
                     /locus_tag="VBC94_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6980297.1"
                     /translation="MFTTFDRVKELSQKRGISLSKLEETLGLGKNSIYALKRNQPSAE
                     RLQEIADYFNVSTDYLLGRTDNPNIAKDNQEYTSDDLRKMAEKAKTFDGKPLTDSDID
                     AIQNIIEIYLKGR"
BASE COUNT          315 a          178 c          148 g          404 t
ORIGIN      
        1 caagtatagt agattttcca gttccgttat gaccagaaat acaagttaca tatttattta
       61 aattaagatg taagtcttca aatcccctga aattttgaag atttatactt tttattgaaa
      121 tcgccatata acttttcctt tttataacct aattagctta ctttatttag aatttttata
      181 tttctaattt aataatgtaa ttgaattatt aagattcaaa acttgttcca caatcagcgc
      241 aataccattg attatactgc atccagcaga ttgtaatact cgtcgataac catgacttca
      301 tcagtcgtgg tttttaagtt gtgccttttc ataaaagata agtaattaaa ctcatgttta
      361 tcttctactt cgtcaaattc atcctcaagt aagcacctga tcataaatct attagcttca
      421 agttcaaatt cctcgtgtct gcgcccatat tggttagagt cgtggtcaat atgtccgagt
      481 tcatggaaga taactttctt ttttgattca tctgatagtg ctttatttac aaagactact
      541 tttatttcgt caacatatat ccctggacgt ggccacaaat cattgtcgaa ataagctaat
      601 tctacattat ggaagtctaa aatctcttca atagtcatag actatcttcc tttcaaataa
      661 atttcaataa tattttgtat tgcatcaata tctgaatcag taagtggctt gccatcaaat
      721 gttttagcct tttctgccat ttttctaaga tcgtcagaag tgtattcttg gttatcttta
      781 gctatattgg ggttatctgt tctacctaat aagtaatctg tagatacgtt gaagtagtct
      841 gctatttctt gtaacctttc agcagatggt tggtttcttt taagagcata gatagaattt
      901 ttgcccaatc ctagagtttc ctctaattta cttagggaaa ttcctcgctt ttgggataat
      961 tctttaactc tatcaaaagt cgtaaacatt gattttatca acctttctag ggtatgacaa
     1021 aaaatattta gagaattttg tagaa
//
LOCUS       DATROQ010000190         1042 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.192, whole genome shotgun sequence.
ACCESSION   DATROQ010000190 DATROQ010000000
VERSION     DATROQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.192"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..100
                     /locus_tag="VBC94_002081"
     CDS             <1..100
                     /locus_tag="VBC94_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEO6980298.1"
                     /translation="KVASVIGYKNKKMVWGHITREKQMKDFETSVT"
     gene            340..840
                     /locus_tag="VBC94_002082"
     CDS             340..840
                     /locus_tag="VBC94_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6980299.1"
                     /translation="MACIIRRARLGDEVNLAYIQTESWKAALGKILPEDIIQKTTEIE
                     PAITMYQQLLHKEVGKGYILEVDSNPHCMAWWDKSREDGMLDYAELICIHSLKEGWGK
                     GYGSQMMNHVLSEIQQAGYNKVILWVFTENTRARKFYDRFGFSFKGKSKTYFEKEEMC
                     YEKILH"
BASE COUNT          401 a          112 c          214 g          315 t
ORIGIN      
        1 taaggttgca agtgttattg gctataaaaa taaaaaaatg gtttgggggc atattacgag
       61 agagaaacaa atgaaggatt ttgaaacaag tgttacttag aagttatagc gattcatata
      121 tataaaataa tcactaaaag agcgtaatta tctacgctct tttagtgatt atcaataatt
      181 ggcctaatct gaggttaatg tttaaaacca tctaaaattt tagaaatata atgataaatg
      241 agaaccagaa ctgttcaaaa taaaaattaa tatcacgaaa atggtataat tggagtagtt
      301 atcaggcagt taataataat ttttgaaagg attttagtta tggcttgcat aatcaggaga
      361 gcaagattag gagatgaagt caatttagct tatattcaaa cggagagctg gaaagctgct
      421 ttgggtaaga tactacctga ggatatcatt caaaaaacta cggaaataga acctgccata
      481 acaatgtatc aacagctact acacaaggag gttggtaagg ggtatatatt ggaagtagat
      541 agcaatcctc attgtatggc ttggtgggat aaaagtagag aagatggtat gcttgattat
      601 gcagaactca tttgtataca tagtcttaaa gaaggttggg gaaaaggtta tgggtctcaa
      661 atgatgaatc atgtactatc agagatacaa caagcaggtt ataataaagt gatactttgg
      721 gtatttacag aaaatacacg cgcaaggaaa ttttacgata ggtttggttt ttcttttaag
      781 ggtaaatcaa aaacttattt tgaaaaggaa gaaatgtgtt atgagaagat tttgcactag
      841 cgagataaag catccgataa tacaatagta taaacatcct attttgaaaa acaatttcct
      901 ttaaggggaa caaatttgta aaagaaaatt gttataggta taatgaagat tatgaaggta
      961 aaattttttg agtgaaaaac agtgtatcaa ttttaggttc tctagtctac aaatagttat
     1021 gctaactcta tggaagaatg ga
//
LOCUS       DATROQ010000191         1015 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.193, whole genome shotgun sequence.
ACCESSION   DATROQ010000191 DATROQ010000000
VERSION     DATROQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.193"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..599
                     /locus_tag="VBC94_002083"
     CDS             <1..599
                     /locus_tag="VBC94_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO6980300.1"
                     /translation="HLIAKRFLDITGAIIGLLICGIVAIFLVPQIRKDGGPAIFSQNR
                     VGRNGRIFRFYKFRSMRVDAEQIKKDLLVHNQMTGLMFKLEDDPRITKIGKFIRKTSI
                     DELPQFYNVLKGDMSLVGTRPPTVDEYEKYNSTQKRRLSFKPGITGLWQISGRNNITD
                     FDEIVKLDVQYINEWSIWSDIKIILLTLKVVLLGTGAK"
     gene            623..>1015
                     /locus_tag="VBC94_002084"
     CDS             623..>1015
                     /locus_tag="VBC94_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HEO6980301.1"
                     /translation="MKICLVGSSGGHLAHLNLLKPIWEKEDRFWVTFDKEDARSILRE
                     EIVYHCFFPTNRNVKNLVKNTILAFKVLRKERPDVIISSGAAVAVPFFYIGKLFGCKT
                     VYIEVFDRIDKPTLTGKLVYPVTDKFIVQ"
BASE COUNT          346 a          137 c          209 g          323 t
ORIGIN      
        1 gtcaccttat agcaaaacga tttttggata tcacgggtgc tattataggt ttgctcatat
       61 gtggcattgt ggcaattttt ctagttccgc aaatcagaaa agatggtgga ccggctatct
      121 tttctcaaaa tagagtaggt cgtaatggta ggatttttag attctataaa ttcagatcaa
      181 tgcgagtaga tgcagaacaa attaagaaag atttattagt tcacaatcaa atgacagggc
      241 taatgtttaa gttagaagat gatcctagaa ttactaaaat aggaaaattt attcgaaaaa
      301 caagcataga tgagttgcct caattctata atgttttaaa aggtgatatg agtttagtag
      361 gaacacgccc tcccacagtt gatgaatatg aaaagtataa ttcaacgcag aagcgacgcc
      421 ttagttttaa gccaggaatc actggtttgt ggcaaatatc tggtagaaat aatattactg
      481 attttgatga aatcgtaaag ttagatgttc aatatatcaa tgaatggtct atttggtcag
      541 atattaagat tattctccta acactaaagg tagttttact cgggacagga gctaagtaaa
      601 ggtaaggttt gaaaggaata taatgaaaat ttgtctggtt ggttcaagtg gtggtcatct
      661 agcacacttg aaccttttga aacccatttg ggaaaaagaa gataggtttt gggtaacctt
      721 tgataaagaa gatgctagga gtattctaag agaagagatt gtatatcatt gcttctttcc
      781 aacaaaccgt aatgtcaaaa acttggtaaa aaatactatt ctagctttta aggtccttag
      841 aaaagaaaga ccagatgtta tcatatcatc tggtgccgct gtagcagtac cattctttta
      901 tattggtaag ttatttggtt gtaagaccgt ttatatagag gttttcgaca ggatagataa
      961 accaactttg acaggaaaat tagtgtatcc tgtaacagat aaatttattg ttcag
//
LOCUS       DATROQ010000192          995 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.194, whole genome shotgun sequence.
ACCESSION   DATROQ010000192 DATROQ010000000
VERSION     DATROQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.194"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..85
                     /locus_tag="VBC94_002085"
     CDS             <1..85
                     /locus_tag="VBC94_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HEO6980302.1"
                     /translation="SQSTIKIRLMRARREVKKYLEKDNFKI"
     gene            82..>995
                     /locus_tag="VBC94_002086"
     CDS             82..>995
                     /locus_tag="VBC94_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor regulator N-terminal
                     domain-containing protein"
                     /protein_id="HEO6980303.1"
                     /translation="MIKTKFSDPLEQLAKKRKRKTLFKTVVLALITVLLVVGIAFKGF
                     TYLTSKNGQKTYHNFELLSEIAYPNISYDSLYYQPSGQFTGKVHADRFKDIDGVQIPY
                     SSFEENYSISGTFGTNADEKSEKNGLYDRGTRQKVPQFFNKNVKYKKGEAKTTPTNDL
                     KYVNKLNNDLIEIAITFDKSYSYKEIKTMIPNNIKQNWLWIGTSTELDTSYWPTKYQF
                     GTDPETLNTPQIFIDKIKENLKGNSKVYFNNINIYSDLEKYAKKYDKVNNSDKLKFSG
                     IILTGKAENFKQLKEKEWIHASSIGANI"
BASE COUNT          447 a          127 c          148 g          273 t
ORIGIN      
        1 atcacaaagt acaattaaaa taagacttat gcgagctaga agagaagtta aaaaatattt
       61 agaaaaggat aattttaaaa tatgataaaa acaaagttta gtgatccact ggaacaatta
      121 gccaaaaaaa ggaaaaggaa aacattattc aagacagtag tattggcatt aataactgta
      181 ctattagtag ttggaatagc ttttaaagga tttacatatt taacttcaaa aaatggtcaa
      241 aaaacatatc ataactttga actactttct gaaatagcat atccaaatat ttcctatgat
      301 agtctctatt accagccaag cggtcagttt actggtaaag tacatgctga tagatttaaa
      361 gatatcgatg gggttcaaat tccatattct agttttgaag aaaattatag tatctcaggt
      421 acttttggca caaatgctga tgaaaaatca gaaaagaatg gcttgtatga tcgaggtact
      481 agacaaaaag ttccacaatt tttcaataaa aatgttaagt ataaaaaagg agaagccaaa
      541 acaactccaa caaatgattt aaaatatgta aataaattga ataatgatct tattgaaatt
      601 gcaataactt ttgataagtc ctatagttac aaagaaataa aaacaatgat accaaataat
      661 ataaaacaaa attggttatg gataggtaca agcacagaat tagatacatc atattggcca
      721 acaaagtatc aattcggtac agatcctgaa actttgaata caccacaaat attcatagat
      781 aaaataaaag aaaacttaaa aggtaactca aaagtatact ttaataatat aaatatctac
      841 tcagatttag aaaagtatgc caaaaaatac gacaaggtaa ataatagcga caaattaaaa
      901 ttttcgggaa taatattaac aggtaaagcc gaaaacttta aacaacttaa agaaaaagaa
      961 tggatacatg cttcaagtat aggggcaaac ataga
//
LOCUS       DATROQ010000193          982 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.195, whole genome shotgun sequence.
ACCESSION   DATROQ010000193 DATROQ010000000
VERSION     DATROQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.195"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..202
                     /locus_tag="VBC94_002087"
     CDS             <1..202
                     /locus_tag="VBC94_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6980304.1"
                     /translation="DNAVTEAFFKYLKHRQINQKNYQNIKQVQLDCFEYIENFYNNYI
                     PHTANLGLTPNQKEENYFNAIK"
     gene            complement(323..655)
                     /locus_tag="VBC94_002088"
     CDS             complement(323..655)
                     /locus_tag="VBC94_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEO6980305.1"
                     /translation="MLRKYKQRNYNVAHTKQRFSIKKFKFGVASVLIGLTFLGMSSHS
                     VLADESSIMSTEVPQTAGVKPEVTVTQPTSDVTSLATDTSTSAVLSTDASHSNPTESA
                     EHAVGLEC"
     gene            838..>982
                     /locus_tag="VBC94_002089"
     CDS             838..>982
                     /locus_tag="VBC94_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEO6980306.1"
                     /translation="MVSSDNTNLKFLKAFSELLKMRSFEQIKVSDLAKKARLSRRSFY
                     NHYN"
BASE COUNT          331 a          167 c          145 g          339 t
ORIGIN      
        1 tgataatgcc gtaactgaag catttttcaa gtatttaaag catagacaaa tcaaccaaaa
       61 aaattatcaa aatatcaaac aggttcaatt agactgcttt gaatacattg agaattttta
      121 taacaattac atcccacata cggctaatct aggactaacc cctaatcaga aagaagaaaa
      181 ttattttaac gcaataaaat aacacggttt tctgtctact tatttgacat tagtccactc
      241 tgtgaattca gatgtggaag aagaagtaac tcgcaatgtt ttatttgctt ctttttcgtg
      301 tgtcactgga accgctgcat cttcagcatt ctaatcctac agcatgctcc gctgattctg
      361 taggattaga atgagatgca tctgttgata acactgctga agttgacgta tctgtcgcaa
      421 gacttgttac atctgaagta ggctgtgtaa cagtaacttc cggttttaca ccagctgttt
      481 gtggtacctc agtactcata atgcttgact catccgccaa aacagaatga ctactcattc
      541 ctagaaatgt caatccaatt aataccgatg ctacaccaaa tttgaatttc ttaatcgaaa
      601 aacgctgttt cgtatgtgcg acattataat ttctttgctt atacttccta agcataactt
      661 ctctcctttt caaaagtaaa aatagattaa ccaataaagt tatctatttt tacttttttt
      721 aaagttttac tgttttacat tatctcgata aatgtgaact attatttgaa tttgtattgt
      781 ataatcacat tcgataagat ttataacttt aagattttat ataaaggtga tagtatattg
      841 gtaagttcag ataacactaa tttaaaattt ttaaaagctt tctcagaatt gttaaagatg
      901 aggagctttg agcaaattaa agttagcgat ttagcaaaaa aagctcgact tagtagaaga
      961 tctttctata atcattacaa tt
//
LOCUS       DATROQ010000194          967 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.196, whole genome shotgun sequence.
ACCESSION   DATROQ010000194 DATROQ010000000
VERSION     DATROQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.196"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            40..963
                     /locus_tag="VBC94_002090"
     CDS             40..963
                     /locus_tag="VBC94_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6980307.1"
                     /translation="MRIQQLQYVIKIVETGSMNEAAKQLYITQPSLSNAVRNLETEMG
                     IQIFIRNPKGITLTKDGMEFLSYARQILEQTALLEERYKGDNTSRELFSVSSQHYAFV
                     VNAFVALFNGTDMTQYELFLRETRTWEIIDDVKNFRSEIGVLFLNSYNRDVLTKLFDD
                     NSLIATTLFTTTPHIFVSKSNPLANRKKLSMKDLEDYPYLSYDQGLHNSFYFSEEMMS
                     QIPHPKSIVVSDRATLFNLMIGLDGYTVATGILNSKLNGDEIVAIPLDVDDVIDIVYI
                     RHDKANLSKMGQKFIDYLLEEVSFNHKNKQS"
BASE COUNT          324 a          165 c          162 g          316 t
ORIGIN      
        1 ctaattgaat ttatttacat tctagtttaa actagaatta tgagaattca acaattacaa
       61 tatgttatta agattgtaga aactggtagc atgaatgaag ccgctaaaca actctacatt
      121 acccaacctt cactttcaaa cgctgttagg aaccttgaga ctgagatggg tatccaaatt
      181 tttattcgaa atccaaaagg aataacgtta acaaaagatg gtatggaatt tctgtcatat
      241 gcgcgacaaa ttctagaaca aacagcactt cttgaagagc gttataaggg tgataataca
      301 agtcgtgagc tgttcagcgt ttcttcacaa cactatgcat ttgtggttaa cgcttttgta
      361 gctctcttta atggcacaga catgacccaa tacgaacttt ttttacgtga aactaggact
      421 tgggaaatta ttgatgatgt taaaaatttc cgttcagaga ttggtgttct gtttcttaat
      481 agctataata gagatgtatt aaccaaatta ttcgacgata attccttaat tgccacaact
      541 ctttttacta ctacacctca tattttcgtg agcaaatcca atcccttagc taatagaaaa
      601 aaattaagta tgaaagattt agaagattat ccttatctga gttatgatca aggcctgcat
      661 aattctttct acttttctga ggaaatgatg tctcaaattc ctcacccaaa atccattgtt
      721 gtcagtgata gagcaactct gtttaattta atgattggat tagatggtta tacagtggca
      781 actggtatcc tcaactcaaa attgaacggt gacgaaattg tcgcaatccc tcttgacgtg
      841 gatgatgtta ttgatattgt ctatatccga cacgacaaag caaacctttc aaaaatggga
      901 caaaaattta ttgactattt attagaggaa gtttcgttta atcataaaaa taaacagtcc
      961 tgagatt
//
LOCUS       DATROQ010000195          946 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.197, whole genome shotgun sequence.
ACCESSION   DATROQ010000195 DATROQ010000000
VERSION     DATROQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.197"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            189..893
                     /locus_tag="VBC94_002091"
     CDS             189..893
                     /locus_tag="VBC94_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980308.1"
                     /translation="MRLVWKFLSEKLVCKIMDSSYGVLILFFICLSITAVGGYTLVYN
                     LSGYSSMDAQKVFGIIVLTIILLVFIVGTIFYAIGSAQLIMSILSKKKISFDEKDAPL
                     DGATHVGETVFQGKEVSLYSKQDKFFLWSRKWQKGFSKYQYYHIRPVDQTEISEVYQV
                     ERCCFYCDHIFTQFFIREGKNREKIVELCTIDVQIGNDRDLGFKQVNRGEFEKTVPFK
                     DVEIKEIKKLIDLKQI"
BASE COUNT          323 a          105 c          190 g          328 t
ORIGIN      
        1 aatatatata tctactgtaa tgttatacga atctaaaata actcagctta cttcaattat
       61 agccatgtag catagaattt tattgagttt ttgatttttg atgacatgag actagatgat
      121 caatatccga atatggtcac tttaatgtgt atagctatta tattaatata tttagggagg
      181 gaagtgttgt gcgccttgta tggaaatttc tatcagaaaa gttagtatgt aagatcatgg
      241 attcttcgta cggtgtgttg atattatttt ttatttgcct atcaataact gctgtaggtg
      301 gatatacatt ggtatataat ttgtctgggt attcctcaat ggatgcacag aaagtttttg
      361 gaatcattgt actaacaata attttactag tatttattgt aggaacaatt ttttatgcaa
      421 taggttctgc acagttgata atgtcgattt tgtctaagaa aaagatttcg tttgatgaga
      481 aagatgcacc acttgatggt gcgactcatg tgggagagac agttttccaa ggaaaggagg
      541 tgtcgttata tagtaagcag gataaatttt tcctatggag tagaaagtgg caaaagggtt
      601 tcagcaaata tcaatattat cacatacgac cggttgatca aactgaaatt agtgaggtgt
      661 atcaagtgga aagatgttgc ttctattgtg accatatatt tacacaattt tttattagag
      721 aaggaaaaaa tagagagaaa atagttgagt tatgcactat tgatgtacaa attggtaatg
      781 acagagatct aggatttaaa caagttaata gaggcgagtt tgaaaaaacg gttcctttta
      841 aggatgtcga aattaaagaa ataaagaaat tgattgattt gaaacaaata taagaaaagc
      901 acctttaaaa gggcttttct tatatttttc tttaaaaagc tcgtta
//
LOCUS       DATROQ010000196          922 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.198, whole genome shotgun sequence.
ACCESSION   DATROQ010000196 DATROQ010000000
VERSION     DATROQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.198"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(105..>922)
                     /locus_tag="VBC94_002092"
     CDS             complement(105..>922)
                     /locus_tag="VBC94_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEO6980309.1"
                     /translation="NLLFSIDDMYVDHFKVMLYSLVRQTKNRKLEIYVLQKTLLKRHT
                     ELIQYTQNLEVGYHPIIVGTEVFAQAPTTDRYPDTIYYRLLAHKFLPETLDRILYLDA
                     DMLCLNDFSSLYDMELGDQLYAAASHNTDGKFLDYVNKLRLKNVELESSYFNTGVLLM
                     NLPAIRKVVHQQTILDYIMQNRGRLILPDQDILNGLYANLVKPIPDEIYNYDARYSLI
                     YQLKSRNEWDLEWVINHTVFLHFAGRDKPWKKDYRGRYSGLYKFMAKEALTKN"
BASE COUNT          321 a          152 c          131 g          318 t
ORIGIN      
        1 ttttcttttt tataaataat tattagcaaa gattattgat atcattaaaa tatcacccca
       61 aaaaaagtta ctaaatagaa ataatttagt aaccttttaa atcttcaatt tttagttaaa
      121 gcctctttag ccataaactt ataaaggccc gagtaacgtc ctctataatc ttttttccat
      181 ggtttatctc gtcctgcaaa atgtaaaaaa acagtatggt taataaccca ctctaaatcc
      241 cattcattgc gactttttaa ttgataaata agactatacc tagcatcata attataaatt
      301 tcatcaggta ttggtttaac caaattagcg tataagccat ttaagatatc ctgatctggt
      361 aagattagac gaccacggtt ttgcattatg taatctaaga tagtttgttg atgaacaact
      421 tttcgtatag caggcaagtt catcaagagt acccctgtat tgaaataaga ggattctagc
      481 tctacatttt ttaaccgtaa tttattgaca taatctagaa acttaccatc agtattatga
      541 cttgccgctg catatagttg atcacctaac tccatgtcat ataaacttga gaaatcattt
      601 aagcaaagca tatctgcatc taaataaagt atcctatcta aggtctcagg taaaaattta
      661 tgagccaaaa gtcgataata tattgtatct ggatagcgat cagttgtagg tgcctgagca
      721 aatacttcgg tacctacaat tattggatga tagccaactt ctaaattttg agtatattga
      781 ataagttctg tatgacgttt gagtaatgtt ttttgtaaga catatatctc taacttacga
      841 ttcttggttt gtcggacaag cgaataaagc atcactttaa aatgatctac gtacatatca
      901 tcaatagaaa ataatagatt ca
//
LOCUS       DATROQ010000197          907 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.199, whole genome shotgun sequence.
ACCESSION   DATROQ010000197 DATROQ010000000
VERSION     DATROQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.199"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..584)
                     /locus_tag="VBC94_002093"
     CDS             complement(<1..584)
                     /locus_tag="VBC94_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEO6980310.1"
                     /translation="MVVYNLNRGIGWASSGVEYAQAYRSEVFRKLGVEAKFIFTDMFQ
                     NENIEHLTRNIGFEDNEIIWLYTFFTDLTIAATSYSLQQLKESFSLPIDRTEKNGKII
                     SFFFKGSSIVVTVMLNDESSNIVQRVEYLMGGKLVRKDYYSYTKMFSEYYAPEDIGPC
                     LYQRTFYNEDGSVAYEENVDGENSIFKFKETILYS"
     gene            complement(600..>907)
                     /locus_tag="VBC94_002094"
     CDS             complement(600..>907)
                     /locus_tag="VBC94_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEO6980311.1"
                     /translation="REFTAKKTKLQSDNEFDNFVRISILKAIDKSWIEEVDSLQQLKG
                     VVTNRGIGQRDIIQEYYKESLNSYYKMGKEIRYSIVKNIMLSTISKASDGSYSIYYV"
BASE COUNT          289 a          178 c          103 g          337 t
ORIGIN      
        1 gaatatagta tagtttcttt gaatttaaaa atactattct cgccatctac attttcttca
       61 tatgcaacac ttccatcttc attataaaat gtacgctgat acaggcatgg acctatatct
      121 tcgggagcat aatattctga aaacatttta gtatatgagt agtagtcttt tctaacaagc
      181 tttcccccca taagatactc taccctttgg acgatgttac tagattcatc gttaagcata
      241 actgtaacta caatactact tcctttaaaa aaaaatgata ttattttacc gtttttctca
      301 gtcctatcaa tcggtagtga aaaactttct ttcaattgtt gcagtgaata agaagttgca
      361 gcaattgtta aatcagtaaa aaaagtataa agccatataa tctcattgtc ttcaaagccg
      421 atatttcgag taaggtgctc tatattttca ttctgaaaca tatcagtaaa aataaattta
      481 gcttcaacac ctaactttct aaaaacttct gaacgatatg cttgagcata ctctacaccg
      541 ctacttgccc aacctatccc tctattcaaa ttataaacta ccattaactc cgttttcctt
      601 tatacataat atattgagta acttccatca cttgcttttg aaattgtact taacataata
      661 ttcttaacga tagaatatct tatttctttt cccatcttat agtaagaatt aagcgattct
      721 ttataatatt cttgaataat atctcgttga ccgatacctc tattggttac aactcccttt
      781 aattgctgta ggctatccac ttcttctatc cacgatttgt caatagcctt taatatcgat
      841 attctgacga aattatcaaa ttcattatca ctttgaagtt tagttttctt tgcagtaaat
      901 tctctat
//
LOCUS       DATROQ010000198          860 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.200, whole genome shotgun sequence.
ACCESSION   DATROQ010000198 DATROQ010000000
VERSION     DATROQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.200"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..>860)
                     /locus_tag="VBC94_002095"
     CDS             complement(<1..>860)
                     /locus_tag="VBC94_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 8 glycosyl transferase"
                     /protein_id="HEO6980312.1"
                     /translation="LDSDIIVNTNLDYLFELDFKGHYLAAVKDPHKNEEGGFNAGMLL
                     ANLELWREDGLTKTLLKTAEELHRVVKTGDQSILNIVCHNRWLSLNKTWNFQTYDVVS
                     RYNHRSYLYLNIENRTPNIIHFLTSDKPWNENSVARFRELWWYYFQLDFCQLTGKQRK
                     VISYEKSMELLSVSDIHLFTLTSSDNLEHIESLICRCPTVQFHIGAYTTVSNKLSKLE
                     QYPNVLVYPELIEARIEKLITLATAYLDINHGPEVGNILQRVHLKQKPIYSFNNTSHQ
                     ENITKHIVNH"
BASE COUNT          269 a          133 c          155 g          303 t
ORIGIN      
        1 tatgattaac gatatgtttg gttatattct cttgatgaga cgtgttgttg aaagaataaa
       61 ttggcttttg tttcaaatga actctttgta aaatattacc tacttctgga ccatgattaa
      121 tatcaaggta tgcagtggct aatgttatta atttttcaat tcttgcctca attaattcag
      181 ggtaaactag gacatttgga tattgttcta gtttgctaag tttatttgac actgttgtgt
      241 aggcaccaat atggaattga acagtaggac atctacaaat tagcgattca atgtgttcca
      301 aattatcgga agatgtaagg gtgaaaagat gaatatctga aacagaaagc aattccatgg
      361 acttttcgta agaaatcact tttctttgct tgccggttaa ttggcaaaaa tcaagttgga
      421 agtaatacca ccatagttct ctaaaccttg caacgctatt ttcattccaa ggtttgtcac
      481 tagttaaaaa atgtataata ttaggagttc tattttctat gtttagatat aaataagatc
      541 gatgattata gcggctaacg acatcataag tttgaaagtt ccatgttttg ttcagagata
      601 accaacgatt atggcaaaca atgttcaaga tactttgatc ccctgttttg acaactcggt
      661 ggagttcttc agctgttttt agtaatgttt tagtgaggcc atcttcccgc catagttcta
      721 gattagctaa aagcatgcca gcattaaacc ctccttcttc atttttatgg ggatctttga
      781 cggctgctaa gtaatgacct ttaaaatcta gttcaaataa gtaatcaaga ttagtgttaa
      841 cgataatatc tgaatctaaa
//
LOCUS       DATROQ010000199          827 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.201, whole genome shotgun sequence.
ACCESSION   DATROQ010000199 DATROQ010000000
VERSION     DATROQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.201"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..332
                     /locus_tag="VBC94_002096"
     CDS             <1..332
                     /locus_tag="VBC94_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000827864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6980313.1"
                     /translation="RNKHLPLTETTYYILLALFEEAHGYAIMKKVEEMSGGDVRIAAG
                     TMYGAIENLLKQKWIKSISSDDRRRKVYIITETGKEIVELETNRLRKLLNTANQLGFG
                     GDGYDKV"
     gene            319..>827
                     /locus_tag="VBC94_002097"
     CDS             319..>827
                     /locus_tag="VBC94_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEO6980314.1"
                     /translation="MIKFKLFFKSTEKEEEWIDKVQKSGYTFTGINSFLPAFYYFKRD
                     KEKDKMSIKMDFKKYMSKDDYTNYITLFEDSGWKHISGSRWGGLHYFQKIKPETSSEI
                     FSDQESKSAMAMSYYKYTCEHAFFSLFYFFLLIRTTSITIFDPKSWYLTQGLWEMQGK
                     IFWLGFLFET"
BASE COUNT          303 a          102 c          165 g          257 t
ORIGIN      
        1 aaaggaataa acatttaccg ttaacagaaa ctacctatta tattttatta gctttgtttg
       61 aggaagcgca tggctatgct attatgaaaa aagttgaaga aatgagtggc ggtgatgtta
      121 gaatagccgc agggacaatg tacggtgcca ttgaaaattt acttaaacaa aaatggataa
      181 agtctatctc aagtgacgat agaagaagaa aagtttatat tattactgag acaggaaaag
      241 aaatagtaga acttgaaacg aatcgattaa gaaagttact taatactgct aatcagttgg
      301 gttttggagg agatggttat gataaagttt aaattatttt ttaaatccac agaaaaagag
      361 gaagaatgga tcgataaggt tcaaaaaagt ggctatactt ttacaggcat taactcattt
      421 cttccagcct tctattattt taagagagat aaagaaaaag acaagatgtc aataaaaatg
      481 gattttaaaa aatacatgtc gaaagatgac tatactaact atatcacttt atttgaggac
      541 agtggatgga agcatattag cggtagtcgt tggggagggc tgcattattt tcaaaaaata
      601 aagcctgaaa ccagttcaga gattttttct gatcaagaat caaaatcagc gatggctatg
      661 agttattata aatatacttg tgagcatgcg tttttctctt tattttattt ctttctcttg
      721 ataagaacaa cttctatcac catatttgat ccaaaaagtt ggtatctgac gcaaggtcta
      781 tgggaaatgc aagggaaaat attttggtta gggttcttat ttgaaac
//
LOCUS       DATROQ010000200          818 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.202, whole genome shotgun sequence.
ACCESSION   DATROQ010000200 DATROQ010000000
VERSION     DATROQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.202"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            235..>818
                     /locus_tag="VBC94_002098"
     CDS             235..>818
                     /locus_tag="VBC94_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEO6980315.1"
                     /translation="MKNISPLRYPGGKSQVYDFVKELVNLNETNTYIEPYMGGMGIAL
                     KLLVNNDVKQIMVNDYDRSIYAFWYSVLNHTNQLIDKIESTPITIEEWKKQRKIQQGK
                     ETCHDLLTLGFSTLFLNRTNRSGIIKAGVIGGLKQDGNYKLDCRFNKEKTIKKIRLIA
                     SYKKQIKLYNIDAEKFIRLNITKTKKSFTFFDPP"
BASE COUNT          333 a          108 c          121 g          256 t
ORIGIN      
        1 tccattttct ggagatttcg acgaatatac taatgaatca aaaaaaataa aagaatggtt
       61 tacaaattat aggtggattt gtgatgaagc aatttattac tacttttcag acaatgaaga
      121 agttatttta cctttcgctt caaaaattga aaaattttac agaaaatcaa taatataata
      181 attctggcca taattaatta aatgttatat cataagtgaa aggaggtaag ccgattgaaa
      241 aatataagcc cgttgagata ccctggaggt aagtctcaag tttatgattt tgttaaagaa
      301 cttgttaacc ttaatgaaac aaatacatat atagaaccgt atatgggagg tatgggaatt
      361 gcattaaagc tacttgttaa taatgatgta aaacaaatca tggtaaatga ctatgatcgt
      421 tcaatctatg ctttctggta ctctgtctta aatcatacaa atcagttaat tgataaaata
      481 gagtccacac ctatcacaat tgaagaatgg aaaaaacaaa gaaaaattca acagggtaaa
      541 gagacatgtc atgatttatt aactcttgga ttttcaactc tatttcttaa tcgaacaaat
      601 cgttctggaa taataaaagc aggtgtcatc ggtggactaa aacaagatgg aaattataaa
      661 ttagattgtc gtttcaacaa agaaaaaact ataaaaaaaa taagattgat tgcatcttat
      721 aaaaaacaaa ttaaacttta taatatagat gctgaaaagt tcattcgttt aaatatcacc
      781 aaaacaaaaa aatctttcac tttttttgac cctccata
//
LOCUS       DATROQ010000201          783 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.203, whole genome shotgun sequence.
ACCESSION   DATROQ010000201 DATROQ010000000
VERSION     DATROQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.203"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            39..176
                     /locus_tag="VBC94_002099"
     CDS             39..176
                     /locus_tag="VBC94_002099"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980316.1"
                     /translation="MELIDEYENYKQTIIVQEENSRTQFLKDRVKLENDISSLEGRKY
                     Y"
     gene            210..491
                     /locus_tag="VBC94_002100"
     CDS             210..491
                     /locus_tag="VBC94_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980317.1"
                     /translation="MSLIGAVLKGTKEDKEFKKQIQKALVRFVHAPEIVEEADSIKTT
                     YSEKIDDISETAKSSIKTIKNQIDTGLVGQFWEKVKAQVDLNKGSYDDL"
BASE COUNT          323 a           89 c          136 g          235 t
ORIGIN      
        1 cttttatgag tttaaatcga ataattgaaa gttatcaaat ggaattaatt gatgaatatg
       61 aaaactataa acagactatc attgtccaag aggaaaatag tcggacacag tttctaaaag
      121 accgagtaaa attagaaaat gacatttcta gtttagaagg aagaaaatac tactaattta
      181 gatggactat aaagtgaaag aggcaataaa tgagtttaat aggcgctgta ttgaaaggaa
      241 ccaaagaaga caaagaattc aaaaagcaaa ttcaaaaagc tttagtaagg tttgtccatg
      301 ccccggaaat agtagaagaa gctgattcta ttaagacgac ttatagtgaa aagattgatg
      361 atatttctga gactgctaaa tcatcaataa aaacgataaa aaaccaaatt gacactggac
      421 ttgtaggaca attttgggaa aaagtaaagg cacaggtaga tttaaataaa ggtagttatg
      481 atgatttata atcgagtgaa aatgttgagg aagcttacta tcctgatagt tattatagat
      541 atgaaaataa tcaagttgga ttagaagaca taacatttta catgaaggat aacaaagttt
      601 attatttaca ttttggtaaa gcaatagata aaaatgataa gtatctcaat caaattataa
      661 ataattaatt tctttatttt ataagccccg aaaacaaatt ttttgataac cttttgataa
      721 cccctccgcg aacatattgt aatacatagt gcacttttgg gatataaatt gttgattttt
      781 agc
//
LOCUS       DATROQ010000202          774 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.204, whole genome shotgun sequence.
ACCESSION   DATROQ010000202 DATROQ010000000
VERSION     DATROQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.204"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..>774)
                     /locus_tag="VBC94_002101"
     CDS             complement(<1..>774)
                     /locus_tag="VBC94_002101"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEO6980318.1"
                     /translation="LESIKSLNDNINIQDVIDIYLPLINLIQIYKRSQENLSFSKAIF
                     LKKENYQRPFIIGISGSVAVGKSTTSRLLQLLISRTFKDSHVELVTTDGFLYPNEKLI
                     QNGILNRKGFPESYDMESLLNFLDTIKNGITAKIPIYSHEIYDIVPNQLQTIETPDFL
                     ILEGINVFQNQQNHRLYMNDYFDFSIYIDAENKQIEEWYLQRFNSLLQLAEADPSNYY
                     HKFTQIPPHKAMELAKDIWKTINLVNLEKYIEPTRNRAD"
BASE COUNT          246 a           98 c          136 g          294 t
ORIGIN      
        1 aatcagccct atttctggtt ggttctatat atttttcaag gttgactaaa ttaatagttt
       61 tccaaatatc tttagctagt tccatagctt tatgtggcgg tatttgtgtg aatttatgat
      121 agtaattact agggtctgct tcggctaatt gtagaagact gttaaatcgt tgtaaatacc
      181 attcttcaat ttgtttattt tcagcatcaa tgtatattga aaaatcaaaa taatcattca
      241 tgtaaagtcg atggttttgt tggttttgaa agacatttat tccttctaaa attaaaaaat
      301 ctggtgtttc aatagtttgc aattgatttg gaacgatatc gtagatttca tgagaataaa
      361 ttgggatttt cgcggttata ccgttcttta tagtatctaa gaaatttaga agtgactcca
      421 tatcatagga ttccgggaaa cctttacggt ttaaaattcc attttgtatg agtttctcgt
      481 taggatataa aaagccatca gtagtgacta attcgacgtg actatcttta aaagttcgtg
      541 aaattagtaa ttgtagtaag cgactggtag ttgattttcc aacagcaact gaccctgata
      601 tgccgataat aaaaggacgt tgataatttt cctttttaag aaatattgct ttagaaaaag
      661 ataaattttc ttgactacgt ttatagattt gaataaggtt aattaaagga aggtagatgt
      721 caataacatc ttgaatatta atattatcgt ttaagctttt gatagattca agct
//
LOCUS       DATROQ010000203          755 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.206, whole genome shotgun sequence.
ACCESSION   DATROQ010000203 DATROQ010000000
VERSION     DATROQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.206"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(37..651)
                     /locus_tag="VBC94_002102"
     CDS             complement(37..651)
                     /locus_tag="VBC94_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6980319.1"
                     /translation="MNKKSFFQCVILLVSFLVLTLAVKSQSDMISYLDNITSAFFQSI
                     RNSDLTNLMTIISTVVSPLTTSLIALVILGYQYFLNQRIAVWLFMLFFGTNALALLLK
                     DIIARHRPMNQLIFDSGYSFPSGHTISTFLLMILVLVVARQRLRRVLSQVVFVIFALV
                     ILASVIFSRLYLENHFLTDILGSLLLGASSYCGLSAIVSLKELQ"
BASE COUNT          297 a          126 c          104 g          228 t
ORIGIN      
        1 aaaggctctt ttataattca ttggtaacta ctatttttat tgtaactctt tcaaagatac
       61 aatagcagac aaaccacaat atgacgatgc tcctagtaac aacgacccta aaatatccgt
      121 taaaaaatga ttttctaaat aaagtcttga aaaaataact gatgcaagga ttactaatgc
      181 aaatattacg aaaactactt gtgacaatac tcttctcaat ctctgtcttg caaccactaa
      241 aacaagaatc attaataaaa acgttgatat cgtatgtcca ctgggaaaac tataacctga
      301 gtcaaaaata agttgattca taggtctatg acgtgcgatg atatctttta aaagcaatgc
      361 caaagcattg gtaccaaaaa ataacataaa tagccacaca gctatccttt ggtttaaaaa
      421 atattgatac ccaaggataa caagtgctat taatgacgtt gtcaaagggc tgacaactgt
      481 tgatattatc gtcattaagt tagttaaatc ggagtttcgt atgctttgaa aaaaagctga
      541 agtgatatta tccaaataac ttatcatatc actttgagat ttaactgcta atgttaacac
      601 taaaaaagaa acaagtagta taacacactg gaaaaaggat ttcttgttca ctacataacc
      661 tcactttaat aaagagataa atcttcatac attatggttt aaaatattta aaaaatgctt
      721 aaagcacgcg ctaacctctt aagtttaaat atgac
//
LOCUS       DATROQ010000204          714 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.209, whole genome shotgun sequence.
ACCESSION   DATROQ010000204 DATROQ010000000
VERSION     DATROQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.209"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..>714
                     /locus_tag="VBC94_002103"
     CDS             <1..>714
                     /locus_tag="VBC94_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEO6980320.1"
                     /translation="LAYISMVLSFILAIVQLIIWVKNMKMHSHLHGKIAKSTSPMILV
                     FPVLVGLLVPTVSLDSTTVSAKGYNFPLAAGSTGTVSQDGTRVQYLKPDTSTYFTSSA
                     YEKEMQKELKKYKSSGTLTITTENYMEVMELIYLYPEQFMDRQIQYTGFVYNEPKHEG
                     YQFIFRFGIIHCIADSGVYGLLTTGNQKSYPDNTWVTVRGTIKSEYNQLLQQNLPVLH
                     IEESRQVSKPNNPYVYRVF"
BASE COUNT          238 a          134 c          113 g          229 t
ORIGIN      
        1 atttggccta tatttcaatg gtcctatctt ttattttagc aattgtacaa ctaattattt
       61 gggttaaaaa tatgaaaatg catagtcacc tacatggtaa aatagctaaa tcaacaagcc
      121 caatgatttt agtatttccc gttttagtag gtttgttagt acctacagtt agtttagact
      181 cgaccacagt ctctgccaag ggatataatt tccctctagc agccggctct actggtactg
      241 tcagtcaaga tgggacacgc gttcaatatt tgaaaccaga taccagcact tattttactt
      301 cttcagccta tgaaaaagaa atgcaaaaag agcttaaaaa atataaaagc tctggtacat
      361 taaccatcac aactgaaaac tacatggaag ttatggaact aatttatctc tatcccgagc
      421 aattcatgga tcgccaaata caatatactg ggtttgttta taacgaaccc aaacacgaag
      481 gatatcaatt tattttccgc tttggaatca ttcattgtat tgctgattca ggagtttatg
      541 gtctactaac aactggaaat caaaaaagtt atccggacaa tacttgggta actgttagag
      601 gtacaattaa atccgagtat aatcaattgt tacaacaaaa cttaccagtc ttacacattg
      661 aagagtctcg tcaagtttct aaacctaata atccttatgt ttatcgtgtc tttt
//
LOCUS       DATROQ010000205          706 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.210, whole genome shotgun sequence.
ACCESSION   DATROQ010000205 DATROQ010000000
VERSION     DATROQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.210"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            38..>706
                     /locus_tag="VBC94_002104"
     CDS             38..>706
                     /locus_tag="VBC94_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980321.1"
                     /translation="MLNSDFIISKSLANYIHHRRLEVGVSSTDLAEISNMSKSDWESF
                     EKNGGAIPLKSKDIILDLLFLERFPKEKECDFIDKLFEEAKENKLWPEKIYQTMGLTP
                     ALSFIAGCEILSDDINNDLEELSKLPKESHLGQLDTSLLLSLLPQQFITKYDYEFVYK
                     LSKVLAQYTSRNKVGSSYTAHSVIEEIYLYLIAKESILYFESLDENSHLQLKELLDYN
                     DEWPF"
BASE COUNT          247 a          100 c          121 g          238 t
ORIGIN      
        1 gataatcata atgaatagga atcaaaagga gaacggaatg ctaaattctg attttattat
       61 tagtaagtca cttgctaact acattcacca tcgtaggtta gaagttggcg tgtcgtcaac
      121 tgatttggca gaaatatcca atatgtctaa atcagattgg gaatcatttg aaaaaaatgg
      181 tggggcaatt cctttgaaat ctaaggatat catacttgac ttattatttt tggagagatt
      241 ccctaaagag aaagaatgtg attttataga taaattattt gaggaagcaa aggaaaataa
      301 actttggcct gaaaagattt accagactat gggacttact cctgctcttt catttattgc
      361 tggttgtgaa atactttctg atgatattaa taatgatttg gaggaattat ctaaactacc
      421 taaagaaagt catctgggac aattagatac ttccctactt ttatcactat tgcctcaaca
      481 atttatcaca aaatatgatt atgagtttgt ttataaatta agcaaagttt tggctcaata
      541 tacatcaaga aataaagttg gttcatctta tacagcgcat agtgtaatag aggaaattta
      601 tctctattta attgcaaaag aatccattct ttattttgaa tctctggacg aaaactccca
      661 tctacaactt aaggaattat tagattataa cgatgagtgg cctttt
//
LOCUS       DATROQ010000206          696 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.211, whole genome shotgun sequence.
ACCESSION   DATROQ010000206 DATROQ010000000
VERSION     DATROQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.211"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(<1..684)
                     /locus_tag="VBC94_002105"
     CDS             complement(<1..684)
                     /locus_tag="VBC94_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-like surface protein"
                     /protein_id="HEO6980322.1"
                     /translation="MFRRSKNNSYDTLQTKQRFSIKKFKFGAASVLIGISFLGGFTQG
                     QFNISTDTVFAAEVISGSAVTLNTNMTKNVQNGRAYIDLYDVKNGKIDPLQLITLNSP
                     DLKAQYVIRQGGNYFTQPSELTTVGAASINYTVLKTDGSPHTKPDGQVDIINVSLTIY
                     NSSALRDKIDEVKKKAEDPKWDEGSRDKVLISLDDIKTDIDNNPKTQSDIANKITEVT
                     NLEKILVPRI"
BASE COUNT          213 a          133 c           91 g          259 t
ORIGIN      
        1 gattcgaggt actagtattt tttctaaatt agtaacttca gttattttat tggcaatgtc
       61 tgattgcgtc ttaggattat tatcaatatc tgttttgata tcatctaaac ttatcaaaac
      121 tttatcgcga cttccctcgt cccatttagg gtcttccgct ttctttttaa cttcatctat
      181 tttatctctc aaagctgaag aattgtaaat agtcaatgaa acgtttataa tatccacttg
      241 tccatcaggc ttcgtatgag gacttccatc tgtcttcaat actgtataat taatactagc
      301 tgcaccaaca gtagtcaatt cagaaggttg tgtgaaataa ttgccgcctt gcctaatgac
      361 atactgagct tttaaatcag gtgaatttaa cgtaattaat tgtaatggat ctattttccc
      421 atttttcaca tcatataaat ctatatatgc tctaccattc tgaacatttt tagtcatatt
      481 tgtgtttaac gtaacagcac ttcctgaaat tacttcagct gcaaacacag tatctgtaga
      541 aatattaaat tgcccttgag taaaacctcc taaaaaacta ataccaatta gtacagaagc
      601 tgcaccaaac ttaaacttct taattgaaaa ccgttgtttc gtctgtaaag tatcataact
      661 gttattttta gaccttctaa acataatatt ctcctt
//
LOCUS       DATROQ010000207          675 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.212, whole genome shotgun sequence.
ACCESSION   DATROQ010000207 DATROQ010000000
VERSION     DATROQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.212"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..>675
                     /locus_tag="VBC94_002106"
     CDS             <1..>675
                     /locus_tag="VBC94_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6980323.1"
                     /translation="EHYNVSATDETTILWNNYYDYQFSNADSIDAFITSTETQTKTLI
                     DQFKKYLNIEPVVYTIPVGSLSKLQRKEWHERKAFSLLTCSRLASEKHIDWLINAVVE
                     ANKVIPELTFDIYGEGGERQKLQEIIAKNKANNYIRLMGHKNLSSVYKDYQVYLSGST
                     SEGFGLTLMEAIGSGLPIIGLDVPYGNQTFIENNLNGYLIPRETPDNPQQISTAFAQY
                     IVALFNS"
BASE COUNT          231 a           96 c          124 g          224 t
ORIGIN      
        1 gagcattata atgttagcgc aactgatgaa actacgattt tatggaataa ttattatgac
       61 tatcaatttt caaatgctga tagcatagat gcttttatca cctcaacaga aacgcaaact
      121 aagacgttga ttgatcaatt taaaaagtat ttaaatattg aaccggtcgt ttatacaatt
      181 cctgtaggaa gtttatcaaa attacagaga aaagagtggc atgaaagaaa agctttttcg
      241 ttgttaactt gctcacgtct agcttcggaa aaacatattg attggttaat taatgctgtg
      301 gtagaagcta ataaagtaat tcctgaatta acctttgata tttatggtga aggtggcgaa
      361 cggcaaaagt tacaagaaat aattgctaaa aataaggcaa ataattatat acgattaatg
      421 gggcataaaa acctatcttc tgtttataaa gattatcaag tttatttatc gggttctacc
      481 agtgaaggtt ttggtttaac cttgatggag gcaatcggat cggggcttcc gataattggt
      541 ttagatgttc cgtatggtaa tcagaccttt attgaaaata atttgaatgg ctaccttatt
      601 ccaagggaaa caccagataa tcctcaacaa atatcaactg cttttgctca gtatattgtt
      661 gctttattta attct
//
LOCUS       DATROQ010000208          674 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.213, whole genome shotgun sequence.
ACCESSION   DATROQ010000208 DATROQ010000000
VERSION     DATROQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.213"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            367..>674
                     /locus_tag="VBC94_002107"
     CDS             367..>674
                     /locus_tag="VBC94_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980324.1"
                     /translation="MSELLDKISSRNNMLEAYKQVKSNKGSAGIDGVTIEQMDDYLHQ
                     NWRETKKLIKERSYKPQPVLRVEIPKPNGGVRNLGIPTAMDRMIQQAIVQVLSPLCEK
                     "
BASE COUNT          242 a          128 c          158 g          146 t
ORIGIN      
        1 gatatgaagg cgtatatagc ggatgagggg gctataaact ctaaagtcca aataggtagt
       61 cgtaacctat atacgtaaat cacgagagta aacgaggaaa gatgtccgac ttatcccgtg
      121 aggtcctgtc aagggtaaaa cctagtaaca acgaacgaca agaagtcagc cgaacccata
      181 gtagtgatga agtttctgta atggaaatgg aacgaagggg tgaacaacta atcgaagtaa
      241 tcctacttca cttgtgtctg taaaacaaac ggtctgatag aactagatgc ggttacgtat
      301 tgactagagg aaggttcacc aatgtaagat atcatgcaga caacgaaaaa agaaaggaat
      361 acgcacatgt cagaattgct agataagata tcatctcgga acaatatgct cgaagcttac
      421 aagcaagtga aatcaaacaa aggttctgct ggtatcgatg gggtcactat cgagcagatg
      481 gatgactatc ttcaccaaaa ttggcgagaa accaagaaac tcatcaaaga gaggagctat
      541 aaacctcaac cggttctcag ggttgaaatc ccaaaaccaa acggaggagt tcgtaaccta
      601 ggtatcccga cggctatgga tagaatgatt cagcaggcca tcgttcaagt tttgagtcca
      661 ctctgcgaaa aaca
//
LOCUS       DATROQ010000209          673 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.214, whole genome shotgun sequence.
ACCESSION   DATROQ010000209 DATROQ010000000
VERSION     DATROQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.214"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            5..>673
                     /locus_tag="VBC94_002108"
     CDS             5..>673
                     /locus_tag="VBC94_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO6980325.1"
                     /translation="MDKEKLDYWKTIITFLHNVLGDNYEIVLHVVDENDIYIGELVNS
                     HISGRTISSPLTTFALDLIKNKVYKEKDFVTNYKAIVSPLNKEVRGSTFFIKNAQNEL
                     EGMLCINLDISAYQNIALDILDLVNLNVNKILSKSPQKISLPQQEEPVEVLSGNIQDI
                     ISEIVDPSLLNQNIHLSQEVKVEIVSKLHEKGVFQLKGAVSKVAEVLNISEPSVYRYL
                     KKIET"
BASE COUNT          234 a          105 c          114 g          220 t
ORIGIN      
        1 cgatatggat aaagaaaaat tagactattg gaagacgata ataacgtttc tccataatgt
       61 tttaggtgat aattacgaaa ttgttttgca tgtagtagat gaaaatgata tctatattgg
      121 tgagttggtt aatagtcaca ttagtggacg aaccataagt tcacctctaa cgactttcgc
      181 ccttgattta atcaagaata aagtttataa ggaaaaagat tttgttacta actataaggc
      241 gattgtcagc cctctcaata aagaagttcg tggatctact ttcttcatca agaatgctca
      301 aaatgaactt gaagggatgt tatgtatcaa ccttgatatt tctgcatacc aaaatattgc
      361 tttggatatt ctagacttag ttaatttaaa tgttaacaaa atactatcta agtcccctca
      421 aaaaatttct ctccctcaac aggaggaacc tgtagaagtt ctatcaggca atattcagga
      481 tattattagt gagattgttg atcctagtct tttaaatcag aatattcatt taagtcaaga
      541 agttaaggta gaaattgttt ctaaacttca cgaaaaaggt gtattccaat taaaaggtgc
      601 cgtttcaaag gtcgctgaag ttctcaacat ctccgaacca agcgtctatc gttatcttaa
      661 aaaaattgaa act
//
LOCUS       DATROQ010000210          654 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.216, whole genome shotgun sequence.
ACCESSION   DATROQ010000210 DATROQ010000000
VERSION     DATROQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.216"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..229
                     /locus_tag="VBC94_002109"
     CDS             <1..229
                     /locus_tag="VBC94_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980326.1"
                     /translation="GFIDPLTDLGEFDIFQMKFKEPVRYLKNKYSGKPYSPHWQNKIE
                     KMRSHQESLREEVKEEIPITSRARNQENNLS"
     gene            290..649
                     /locus_tag="VBC94_002110"
                     /pseudo
     CDS             290..649
                     /locus_tag="VBC94_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
BASE COUNT          258 a           80 c          118 g          198 t
ORIGIN      
        1 aggctttatt gatccactaa ccgacttggg ggaatttgat atttttcaaa tgaagttcaa
       61 agaacccgtt agatacttga aaaataaata ttcaggaaag ccttattcac cacattggca
      121 gaataaaatt gaaaaaatga ggtctcatca agaaagccta agagaagaag ttaaagaaga
      181 gattccgatt actagtaggg caagaaatca agaaaataat ttgagttgat cttaaattga
      241 taaaaagttc gacttttttc ctaaaaaata gtgtgctata agtcaattaa aagatgtgct
      301 atatttattt aatagatatc gtctcattga atatcaacta accggagaaa attcgaatgc
      361 aaaaaattta atgacaggca ctcaaaattt aaatgctaat tttgaagatg aaaaatcgtc
      421 gatggtatat tatgaaaata tggtggctca ttatataaga ctaacaaacc atcatgtgag
      481 ataccgtgtt acactcttat ttaaaaatat tgagatggtt gcaagagggg ttggtatgga
      541 aacccaaagt gtggaagata atcagatatc ttttgatatt tatattttta atgtgcagtc
      601 aggatacaac attaattatt taacaagata ttccgttaag gaaaattaaa ggag
//
LOCUS       DATROQ010000211          618 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.217, whole genome shotgun sequence.
ACCESSION   DATROQ010000211 DATROQ010000000
VERSION     DATROQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.217"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            48..542
                     /locus_tag="VBC94_002111"
     CDS             48..542
                     /locus_tag="VBC94_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEO6980327.1"
                     /translation="MIKWNEKETKLVDTSFSIMLYLKTLFAVGQVVAGVLLYYFKPTQ
                     IKHLLFLLTEGEVHGGIHGFISAHFLKISSYINDSASHLAAIYLLIHGGIKLVALILL
                     HKEKLFAYPLSILVFVGFIIFQLREYFSSHSILMIFLCVFDVIMIILTWLEYQKMKLN
                     FSKR"
BASE COUNT          205 a           80 c          100 g          233 t
ORIGIN      
        1 atttaagcaa aaaaatggat aatatgattg aggaaaggag gtaagcaatg attaaatgga
       61 atgaaaaaga aacaaaacta gttgatacaa gtttttcaat tatgttgtat ctaaaaacgc
      121 tatttgcagt cggacaagta gtagctggcg ttttattata ttattttaaa cctacacaaa
      181 taaaacatct cctatttctt ttaactgaag gtgaagttca cggtgggatt catggtttta
      241 tttctgctca tttcttgaaa ataagcagtt atattaatga ttcggctagt catttagccg
      301 ctatctatct attaattcat ggcggtatca aattagtagc acttatttta ttacataaag
      361 agaaattatt tgcttaccct ttatcaattt tagttttcgt tgggtttatc attttccaac
      421 taagggaata tttttctagt catagcattt taatgatttt tctatgtgta tttgatgtta
      481 tcatgataat tttaacatgg ttagaatatc agaaaatgaa attgaatttt tcaaaaaggt
      541 gacttccccc atgccttagc gcttgttttt ataagcgctt ttttgctaca atatgactat
      601 gaaaactatt gaagaaat
//
LOCUS       DATROQ010000212          614 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.218, whole genome shotgun sequence.
ACCESSION   DATROQ010000212 DATROQ010000000
VERSION     DATROQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.218"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(12..>614)
                     /locus_tag="VBC94_002112"
     CDS             complement(12..>614)
                     /locus_tag="VBC94_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS861 family transposase"
                     /protein_id="HEO6980328.1"
                     /translation="MKSMGLTARIRRKRKYASYKGEVGKKADNLIQRQFEGSKPYEKC
                     YTDVTEFALPEGKLYLSPVLDGYNSEIIDFTLSRSPALKQVQTMLERAFPAASYSETI
                     LHSDQGWQYQHKSYHQFLEDKGIRPSMSRKGNSPDNGMMESFFGILKSEMFYGLEKSY
                     KSLDDLEQAITDYIFYYNNKRIKAKLKGLSPVQYRTKSFT"
BASE COUNT          169 a          136 c          131 g          178 t
ORIGIN      
        1 gacaatttat tttaagtgaa ggatttagtt ctgtattgca caggactaag tccttttagt
       61 tttgctttga ttcgcttgtt gttgtagtaa aaaatgtaat ctgtgatagc ttgctcaagg
      121 tcatcaagtg atttgtaaga cttctccaag ccatagaaca tctcagactt tagaatccca
      181 aagaaagatt ccatcatacc gttgtcagga ctgttcccct tccgtgacat agaaggacga
      241 atgcctttgt cctccagaaa ttgatggtag gacttgtgct ggtactgcca gccttggtcg
      301 ctgtggagaa tggtttcgct gtatgaagcc gcaggaaaag ccctctcaag catggtttga
      361 acctgtttca aggcaggaga ccgagacagg gtaaaatcaa ttatctcact gttatagcca
      421 tcaagaacag gcgatagata aagtttccct tcaggtaaag caaactccgt cacatcggta
      481 tagcacttct catagggctt cgacccctca aactggcgtt gaatcaggtt atcagccttc
      541 ttaccaacct cacctttgta agaggcatac ttgcgcttac gacgaatacg agcagtcaag
      601 cccatacttt tcat
//
LOCUS       DATROQ010000213          597 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.219, whole genome shotgun sequence.
ACCESSION   DATROQ010000213 DATROQ010000000
VERSION     DATROQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.219"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            36..509
                     /locus_tag="VBC94_002113"
     CDS             36..509
                     /locus_tag="VBC94_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HEO6980329.1"
                     /translation="MIFVTVGTHEQQFNRLIKEVDRLKGTGAIDQEVFIQTGYSDFEP
                     QNCQWSKFLSYDDMNSYMKEAEIVITHGGPATFMSVISLGKLPVVVPRRKQFGEHIND
                     HQIQFLKKIAHLYPLAWIEDVDGLAEALKRNIATEKYQGNNDMFCHKLEKIIGEI"
BASE COUNT          200 a           82 c          119 g          196 t
ORIGIN      
        1 tatcctaagg caattaattt aggaggaatt ttttaatgat ttttgtcaca gtggggacac
       61 atgaacagca gttcaaccgt cttattaaag aagttgatag attaaaaggg acaggtgcta
      121 ttgatcaaga agtgttcatt caaacgggtt actcagactt cgaacctcag aattgtcagt
      181 ggtcaaaatt tctctcatat gatgatatga actcttacat gaaagaagct gagattgtta
      241 tcacacatgg cggcccagcg acgtttatgt cagttatttc tttagggaaa ttaccagttg
      301 ttgttcctag gagaaagcag tttggtgaac atatcaatga tcatcaaata caatttttaa
      361 aaaaaattgc ccacctgtat cccttggctt ggattgaaga tgtagatgga cttgcggaag
      421 cgttgaaaag gaatatagct acagaaaaat atcagggaaa taatgatatg ttttgtcata
      481 aattagaaaa aattataggt gaaatatgag gaaatatcta gatttagatt attctttatt
      541 ttatgctctt tgggtactta ttttagtacc aaaccaatgg tatcagtttt taattat
//
LOCUS       DATROQ010000214          589 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.220, whole genome shotgun sequence.
ACCESSION   DATROQ010000214 DATROQ010000000
VERSION     DATROQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.220"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            62..>589
                     /locus_tag="VBC94_002114"
     CDS             62..>589
                     /locus_tag="VBC94_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6980330.1"
                     /translation="MDILEIKNVNYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKVQTGKILFKNEDIEKKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSVDESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKELA"
BASE COUNT          248 a           65 c          105 g          171 t
ORIGIN      
        1 ttcattaata ttatttaaga aaccaaaaga aatattatca aaaataagtt aggagcaaat
       61 aatggatata ttagaaataa agaatgtaaa ttacagttac gcaaattcta aagaaaaagt
      121 tttgtcagga gtaaatcaaa aatttgaact tggaaagttt tatgcgatag tagggaagtc
      181 aggaacagga aaatccacac ttctttcctt acttgcagga cttgataaag ttcaaacagg
      241 aaaaatcttg tttaagaatg aagatataga aaagaaagga tatagtaatc acagaaaaaa
      301 taatatatct ttggtatttc aaaattataa tttaatagat tatttatcgc cgattgaaaa
      361 tattagacta gtaaataaat cagtagatga gagtatcttg ttcgaattag gtttagataa
      421 aaaacaaata aaaagaaatg ttatgaaatt atctggtggt cagcaacaaa gggtagctat
      481 tgctagggca ctggtatcag atgccccaat aatactagct gatgagccta ccggtaacct
      541 agacagtgtt actgctggag aaataattaa tatattaaag gaattagct
//
LOCUS       DATROQ010000215          574 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.221, whole genome shotgun sequence.
ACCESSION   DATROQ010000215 DATROQ010000000
VERSION     DATROQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.221"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            48..533
                     /locus_tag="VBC94_002115"
     CDS             48..533
                     /locus_tag="VBC94_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980331.1"
                     /translation="MRETETHDHQALIQKLLVSIHYLTLFRDEIILVEKTPSLLGKHF
                     SIAIVQNELGEILSKIEALSKQKKLIRSIYWYDESSFKVMNKALAIVEEWIKGLDNLL
                     EFCQSQTVFQAILGDERAHVFGILIDVYTSLNIINTSLKEENSRPVSLSDLALGLKDE
                     D"
BASE COUNT          198 a           78 c          107 g          191 t
ORIGIN      
        1 aagctacgta tagttttacc agtcagaaat agtaaggatg gttgagtatg agagaaactg
       61 aaacacacga tcaccaagcg ctaattcaaa aattgttagt cagtattcat tatttaacct
      121 tgtttcgtga tgaaattata ttagtagaaa aaaccccatc tttactgggg aaacattttt
      181 ctattgctat tgtgcagaat gaactaggtg agatactttc taaaatagaa gctttgagta
      241 agcaaaaaaa actgattcgt tctatttatt ggtatgatga atcgtcattt aaagtcatga
      301 ataaggctct ggctattgta gaagaatgga ttaagggctt agataatctt ttagaattct
      361 gtcaatcaca gacggttttt caagcaattt taggtgatga acgtgcacat gtatttggaa
      421 tccttattga tgtttatact tctttaaaca ttataaatac ttctttgaaa gaagaaaatt
      481 ctagaccagt gtccttatct gatttggctc taggtcttaa ggacgaagat taataaaaaa
      541 acgttactgc tttgaatgaa taaagcagta acgt
//
LOCUS       DATROQ010000216          573 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.222, whole genome shotgun sequence.
ACCESSION   DATROQ010000216 DATROQ010000000
VERSION     DATROQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.222"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            172..372
                     /locus_tag="VBC94_002116"
     CDS             172..372
                     /locus_tag="VBC94_002116"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEO6980332.1"
                     /translation="MNLAILALGFAVMGVSIGEGILVANIAKSAARQPEMFSKLQTLM
                     FTGVAFIEGTFFVLFAFTFLVR"
     gene            404..>573
                     /locus_tag="VBC94_002117"
     CDS             404..>573
                     /locus_tag="VBC94_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEO6980333.1"
                     /translation="MESTSNPTVSFLGIDFDLTILAMSLLTITIIFILVFWASRKMTI
                     KPKGKQNVLEYV"
BASE COUNT          196 a           83 c          108 g          186 t
ORIGIN      
        1 ataagggatt aaattaaagc taaaaaaact aaaaattgtt atttgaaaag gattgtgtaa
       61 accatctcaa actatttcat gaaaacgtaa tcaaatgagg aaattcattg acatgcacaa
      121 gaaataggaa taaaataacg gagtaaatat tttttaaagg agaattctat tatgaattta
      181 gctattttag cacttggttt cgccgtgatg ggtgtttcaa tcggtgaagg aattttggtt
      241 gcaaatattg ccaaatcagc ggctcgtcag cctgaaatgt ttagcaaact gcaaacattg
      301 atgtttacag gtgtggcctt cattgaaggt accttcttcg ttctatttgc gtttacattc
      361 ttagtaagat agacgacaat ttagaaaaaa ggagggatga ggattggaat ctacaagtaa
      421 tccaaccgta tcttttttag gcatcgattt tgatttaacc atcctagcta tgtctctctt
      481 gacaattact attatcttta tcttagtctt ctgggctagt cgtaagatga caataaaacc
      541 aaaaggtaag cagaatgtct tagaatatgt cta
//
LOCUS       DATROQ010000217          541 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.223, whole genome shotgun sequence.
ACCESSION   DATROQ010000217 DATROQ010000000
VERSION     DATROQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.223"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(1..73)
                     /locus_tag="VBC94_002118"
     tRNA            complement(1..73)
                     /locus_tag="VBC94_002118"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37..39),aa:Ile,seq:cat)
     gene            complement(93..166)
                     /locus_tag="VBC94_002119"
     tRNA            complement(93..166)
                     /locus_tag="VBC94_002119"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Met,seq:cat)
     gene            complement(182..255)
                     /locus_tag="VBC94_002120"
     tRNA            complement(182..255)
                     /locus_tag="VBC94_002120"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(219..221),aa:Pro,seq:tgg)
     gene            complement(263..336)
                     /locus_tag="VBC94_002121"
     tRNA            complement(263..336)
                     /locus_tag="VBC94_002121"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Arg,seq:acg)
     gene            complement(348..432)
                     /locus_tag="VBC94_002122"
     tRNA            complement(348..432)
                     /locus_tag="VBC94_002122"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(396..398),aa:Leu,seq:taa)
     gene            complement(441..512)
                     /locus_tag="VBC94_002123"
     tRNA            complement(441..512)
                     /locus_tag="VBC94_002123"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(478..480),aa:Gly,seq:gcc)
BASE COUNT          150 a          153 c          122 g          116 t
ORIGIN      
        1 ggaccttgta ggactcgaac ctacgaccgc tcggttatga gccgagtgct ctaaccagtt
       61 gagctaaagg tccaaagtct caataaaata aatagcggcg gaggggatcg aacccccgac
      121 ctcccgggta tgaaccggac gctctagcca gctgagctac accgccataa aaatatatcc
      181 atcgggaaga caggattcga acctgcgaca ccttggtccc aaaccaagta ctctaccaag
      241 ctgagctact tcccgaaaaa tatgcaccct agaggagtcg aacctctaac cgcctgattc
      301 gtagtcaggt actctatcca gttgagctaa gggtgctaaa tattatatgc cgaggaccgg
      361 aatcgaaccg gtacgatgtt taccatcgca ggattttaag tcctgtgcgt ctgccagttc
      421 cgccaccccg gcctctaaca agcgaacgac ggggttcgaa cccgcgaccc tcaccttggc
      481 aaggtgatgt tctaccactg aactacgttc gcactaaaga cactatttat cctataaaat
      541 t
//
LOCUS       DATROQ010000218          528 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.225, whole genome shotgun sequence.
ACCESSION   DATROQ010000218 DATROQ010000000
VERSION     DATROQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.225"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
BASE COUNT          211 a           76 c           80 g          161 t
ORIGIN      
        1 tcaagacagc aataaaattg aaaaaatcaa taagataaaa aggatttaaa aaattgatta
       61 ttattgtgga agggataagc ttaatgatga ttttaacagc atgtgactta gtaaaaaaag
      121 atcaccaatg ggttcaagaa cattgaaaat acttagaagc tggatcaaaa gttatcaaag
      181 aagtcagcca aaacattcaa ccagttattt ctgatctgaa tagctatctc aatggagcga
      241 caagtgcttt tgagcaatcg gattcgagtg cagctactca tattaaaaat aacaccatcg
      301 cccaacttaa aaaaagcagt accaaatcgt gtgagaaatt accaggctaa ttgtgagtga
      361 gaatctattg ttctcacttt tttattatca aaacgcttaa attctttact tagacctcgc
      421 ctacacactt cagataatgg gtgttaccat attaattaat aaatatgtaa aaaataatat
      481 ttgatatgct ataataataa tatatttgat taatcaaaat tttttagt
//
LOCUS       DATROQ010000219          519 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.227, whole genome shotgun sequence.
ACCESSION   DATROQ010000219 DATROQ010000000
VERSION     DATROQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.227"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..>519
                     /locus_tag="VBC94_002124"
     CDS             <1..>519
                     /locus_tag="VBC94_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
                     /protein_id="HEO6980334.1"
                     /translation="NSTRIRLNLERIIELRNISTHYITEDYELKYAPLFQACVLNYVN
                     ELQRFHSRDVTKAISQNFLTITATYEPLSNEQIRLKYPAEIAEKFIQQANAIDVLVTE
                     YNSDKFAIGIKQNLYITKKKSEADFIVSIANQSPSQVAILKDPSETHKYSYANIISVV
                     NDRLNKKNIKLN"
BASE COUNT          203 a           90 c           65 g          161 t
ORIGIN      
        1 caacagcact agaattcgac ttaatcttga gcgcattatt gaactaagga atattagtac
       61 tcactacata acagaagatt atgaattaaa gtatgctcca cttttccaag cttgtgtact
      121 taactatgtc aatgaacttc aacgatttca ttcaagagac gttacaaagg ccatttccca
      181 aaatttttta actatcactg caacttacga accattatct aacgaacaaa ttagattaaa
      241 atatcctgcg gaaattgctg aaaaattcat ccaacaggca aatgccatag atgtcttagt
      301 gacagagtat aattctgata aattcgctat tggtattaaa caaaatcttt atattactaa
      361 gaaaaaatct gaagccgatt ttatagtatc aattgcaaat caatcacctt ctcaagttgc
      421 tattttaaaa gatccatcag aaacgcacaa atattcatat gcaaatatta tatctgttgt
      481 aaatgataga ctaaataaaa agaatattaa acttaatta
//
LOCUS       DATROQ010000220          504 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.228, whole genome shotgun sequence.
ACCESSION   DATROQ010000220 DATROQ010000000
VERSION     DATROQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.228"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..>504
                     /locus_tag="VBC94_002125"
     CDS             <1..>504
                     /locus_tag="VBC94_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEO6980335.1"
                     /translation="ETAIVQLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNIIQ
                     DGDTESLIRKYFHSGVVINGQRHKTLVGTPQGGNLSPLLSNIMLNELDKGLEKRGLRF
                     VRYADDCVITVGSEAAAKRVMHSVSSYIEKRLGLKVNMTKTKIVRPNKLKYLGFGFWK
                     SPKGWKCR"
BASE COUNT          143 a           99 c          124 g          138 t
ORIGIN      
        1 gaaacagcca ttgttcagct acttgagtat ttaaacgatg gctacgagtg gattgtggac
       61 attgacttgg aaaagttctt cgatactgtt ccgcaagaca gattgatgtc cctggttcat
      121 aatatcattc aagatggcga tacggagtca ctgattcgta agtacttcca ttcgggagtt
      181 gttattaacg gacagcgaca taagacttta gtcgggacac ctcaaggcgg gaatctatca
      241 cccctcctat ctaatattat gcttaatgag ttagacaaag ggttggaaaa gcgaggtctt
      301 cgctttgtcc gttacgccga tgactgtgtc atcactgtcg gaagcgaagc agctgctaag
      361 cgggtcatgc attcggtcag tagctatatt gagaagcgat tagggttgaa agtcaacatg
      421 actaagacca agattgtcag accgaacaaa ctcaaatacc tcggatttgg tttctggaaa
      481 tctccaaaag gttggaagtg tcgt
//
LOCUS       DATROQ010000221          444 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.232, whole genome shotgun sequence.
ACCESSION   DATROQ010000221 DATROQ010000000
VERSION     DATROQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.232"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            223..>444
                     /locus_tag="VBC94_002126"
     CDS             223..>444
                     /locus_tag="VBC94_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HEO6980336.1"
                     /translation="MESHLVRIINRLELMATDGGNLKRNFEREGVVVAEVSFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDL"
BASE COUNT          158 a           55 c           89 g          142 t
ORIGIN      
        1 atagcaccta aagcatagaa atcaagaaaa aagttctgtt tttagcactg aatatattag
       61 agtgctaaat tgaaaaatag atgactactg agaaaaatcg ttatttgcgg attttagacg
      121 ataaaatcgt taaaaagtta gtaaaaatgt tgcttttcat gttgctttgg tcacttttgt
      181 gttaaaatag aaaagactag aaaatatata ggagtaagta agatggaatc acatttagtg
      241 agaatcatca atcgcctcga attaatggcg actgatggtg gtaatttaaa acgtaatttt
      301 gaacgtgaag gtgttgttgt agcagaagta tcttttagta atgaccctga aaacggtcct
      361 gttttcacat tacgtgatgt agaagcgcgt gaatcatatt catttgacag cattgacctt
      421 atcgcaatgg aaatttacga ttta
//
LOCUS       DATROQ010000222          444 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.233, whole genome shotgun sequence.
ACCESSION   DATROQ010000222 DATROQ010000000
VERSION     DATROQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.233"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(17..394)
                     /gene="mscL"
                     /locus_tag="VBC94_002127"
     CDS             complement(17..394)
                     /gene="mscL"
                     /locus_tag="VBC94_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEO6980337.1"
                     /translation="MIKELKEFLFKGNVLDLAVAVILGAAFNAIITSLVKDVITPLIL
                     NPVLKAAGVSNIAQLSWNGVAYGNFLSAVINFLIVGTTLFFIVKAANKVMAKKPAEEE
                     IIEVVEPTQEQLLAEIRDLLANK"
BASE COUNT          154 a           78 c           72 g          140 t
ORIGIN      
        1 taatccgagt cttttattat ttgttagcga gtaagtcgcg gatttcagca agtaattgct
       61 cttgtgttgg ttcaacaact tcaataattt cttcttcagc aggtttctta gccataacct
      121 tgttagcagc tttaacgatg aagaataaag tagtaccaac gattaagaaa ttaataactg
      181 cacttaagaa attaccgtaa gcaacaccat tccatgaaag ctgtgcgata tttgaaacgc
      241 cagcagcttt taatacagga ttaaggataa gcggtgtaat aacgtcttta acaagtgaag
      301 tgatgatagc attaaaagct gcacctaaaa taacagcaac agcaaggtct aaaacattac
      361 ccttaaataa aaattctttt agttctttaa tcatgtgaac aagctcctta atattattta
      421 attcacgttc attatagcag actt
//
LOCUS       DATROQ010000223          440 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.234, whole genome shotgun sequence.
ACCESSION   DATROQ010000223 DATROQ010000000
VERSION     DATROQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.234"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            34..>440
                     /locus_tag="VBC94_002128"
     CDS             34..>440
                     /locus_tag="VBC94_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO6980338.1"
                     /translation="MKLSYEDKLEIYELRKIGMSWSQISQRYDVRISNLKYMIKLMDR
                     YGVEIVEKGRNEYYPPELKQEMIDKVLIHGCSQLSVSLDYALSNCSILTNWLSQFKKN
                     GYTIVEKTRGRPSKMGRKRKKTWEEMTELERLQ"
BASE COUNT          155 a           63 c           95 g          127 t
ORIGIN      
        1 gacaaaaaat ctaacgattg gggtgctttt ctgatgaaat taagttatga agataaacta
       61 gaaatatacg agttaagaaa gatcgggatg tcctggtctc agattagtca acgatatgat
      121 gttcgtatct cgaatcttaa atacatgata aaactcatgg atcggtatgg tgtagaaatc
      181 gtcgaaaaag gtagaaatga gtattatcca cctgaattaa agcaggaaat gatagataaa
      241 gtcttgattc atggttgttc tcaactctct gtttctcttg attatgctct ctctaattgt
      301 tctatactca caaattggct ttcccaattt aagaaaaatg gttatactat tgttgagaaa
      361 acaagaggga gaccgagtaa aatgggacgt aagcgtaaga aaacttggga agaaatgaca
      421 gagctcgagc gacttcagga
//
LOCUS       DATROQ010000224          435 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.235, whole genome shotgun sequence.
ACCESSION   DATROQ010000224 DATROQ010000000
VERSION     DATROQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.235"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            70..>435
                     /locus_tag="VBC94_002129"
     CDS             70..>435
                     /locus_tag="VBC94_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6980339.1"
                     /translation="MKEKVTVIIPIYNSEAYLKECVQSVLQQTHPLIEVILIDDGSTD
                     NSGEICDNLSQEDNRILVFHKKNGGVSSARNLGLDKSTGEFITFVDSDDFVAPNMIEI
                     MLKNLITENADIAEVDFDIS"
BASE COUNT          162 a           57 c           83 g          133 t
ORIGIN      
        1 aatagatcct aatcattgga gtattgtatt attattctca acttttggta tagtgggaag
       61 ggctaaaaaa tgaaagaaaa agtaacagtc attataccta tatacaactc agaagcatac
      121 cttaaagaat gtgtgcaatc cgtactacaa cagactcatc cattgataga agttatacta
      181 attgatgatg gatccactga taatagtgga gaaatttgtg ataatttatc tcaggaagat
      241 aatcgcatac ttgtatttca taaaaaaaat ggaggggtct cttcggcaag gaacctaggt
      301 ctagataaat ccacaggaga attcataaca tttgtggata gtgatgattt tgtagcaccg
      361 aatatgattg aaataatgtt aaaaaattta atcactgaga atgctgatat agcagaagta
      421 gattttgata tttcg
//
LOCUS       DATROQ010000225          376 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.238, whole genome shotgun sequence.
ACCESSION   DATROQ010000225 DATROQ010000000
VERSION     DATROQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.238"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..>376
                     /locus_tag="VBC94_002130"
     CDS             <1..>376
                     /locus_tag="VBC94_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEO6980340.1"
                     /translation="KQILEIYTKANKRLGVKSIKVILQRDYDTKISEGRIYHLMKNMA
                     LPKMATVKPKTALKKTQKTYPQNLLNQKFYPDKPNQVWSTDFTYISIGYKKYVYLCAI
                     LDLYSRKCIAWKLSHRMDAKLAC"
BASE COUNT          142 a           73 c           57 g          104 t
ORIGIN      
        1 aaaacaaata cttgagattt ataccaaagc aaacaaaaga cttggtgtga agtctatcaa
       61 agtcattctt caaagagact acgacacaaa aatctctgaa ggaagaattt accatctgat
      121 gaagaatatg gcgctcccta aaatggctac cgttaagcca aaaacagctc ttaaaaagac
      181 tcaaaaaacg tatcctcaaa acttactcaa ccagaaattt tatcctgaca aacctaatca
      241 agtatggtct actgacttca cctatatttc tattggatat aagaaatatg tctatctctg
      301 cgcaatactt gatctctatt ctagaaaatg tattgcttgg aaattgagtc accgtatgga
      361 tgcaaagtta gcatgt
//
LOCUS       DATROQ010000226          366 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.239, whole genome shotgun sequence.
ACCESSION   DATROQ010000226 DATROQ010000000
VERSION     DATROQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.239"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..>366
                     /locus_tag="VBC94_002131"
     CDS             <1..>366
                     /locus_tag="VBC94_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEO6980341.1"
                     /translation="EKLLVESREGIPLNKETFWKIDRVLSNGVKKGQRIYHILKTNDL
                     EVSSSTVYRHIKKGYLSITPIDLPRAVKFKKRRKSTLPPIPKAIKEGRRYEDFIEHMN
                     QSELNSWLEMDTVIGRIGGK"
BASE COUNT          129 a           77 c           79 g           81 t
ORIGIN      
        1 gaaaaacttt tagttgaatc tagagaagga atccctttga acaaagagac cttctggaaa
       61 atagatagag tgctttctaa tggggtcaag aagggccaac gtatctatca tatcctcaaa
      121 accaacgatc tagaagtgag ttcttcaacc gtttatcgac acatcaagaa aggctaccta
      181 tccatcacac caatcgacct acccagagct gtgaagttca aaaaaaggcg aaagagcaca
      241 ctccctccta ttccaaaagc gattaaagaa gggcgacggt acgaggattt tatagaacac
      301 atgaaccaat cagagctgaa ctcctggctt gaaatggaca cggttattgg acggattggt
      361 ggaaag
//
LOCUS       DATROQ010000227          338 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.241, whole genome shotgun sequence.
ACCESSION   DATROQ010000227 DATROQ010000000
VERSION     DATROQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.241"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..>338
                     /locus_tag="VBC94_002132"
     CDS             <1..>338
                     /locus_tag="VBC94_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEO6980342.1"
                     /translation="LTMRKWSIDLITRIERLNWVIRGWINYFSLGNMKSIMTQIDERL
                     RTRIRVIIWKQWKKKAKRLWGLLKLGVARWIADKVSGWGDHYQLVAQKSVLKRAISKP
                     ALAKRGLVSC"
BASE COUNT          106 a           63 c           90 g           79 t
ORIGIN      
        1 aactgacgat gaggaaatgg agcattgacc tgataactcg cattgaacga ttgaactggg
       61 tcattcgagg atggataaac tatttctcgc ttggcaatat gaagagtatc atgacacaaa
      121 tagatgagcg tctgcgaacc cgtattcgag tgattatctg gaagcaatgg aagaagaaag
      181 caaagcgcct atggggactc ttaaaactag gagttgctag atggatagcc gataaagttt
      241 ctggatgggg tgaccactat cagttggtag ctcagaagtc ggtactcaaa cgtgctatat
      301 caaaaccagc cctcgcaaag cgaggactgg tcagttgc
//
LOCUS       DATROQ010000228          337 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.242, whole genome shotgun sequence.
ACCESSION   DATROQ010000228 DATROQ010000000
VERSION     DATROQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.242"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            <1..>337
                     /locus_tag="VBC94_002133"
     CDS             <1..>337
                     /locus_tag="VBC94_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980343.1"
                     /translation="SLSLLEYINDTSNDKVTINYLTKHTGQINVVENPKNDYLRRDLM
                     LKLGQPIKQSISVLTEDDTARWYLAHLLNYYVQKRPNTKLQIDTLKMVDCHIGWSSIV
                     SLVKNDFDY"
BASE COUNT          126 a           43 c           53 g          115 t
ORIGIN      
        1 atagcttgtc attattagaa tatattaatg atacttcaaa tgacaaagtt actattaatt
       61 atttaacaaa acatactggt caaattaatg ttgtagaaaa tcctaaaaat gattatttaa
      121 gacgtgattt aatgttaaaa ctcggtcagc cgattaagca atccatttca gttttaacgg
      181 aagatgatac ggcaaggtgg tatctagctc atttacttaa ttattacgtt cagaagcgac
      241 caaatacaaa acttcaaatt gacacattaa aaatggttga ttgtcatatt ggatggagca
      301 gtattgtaag tcttgtaaaa aatgattttg attactt
//
LOCUS       DATROQ010000229          314 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.243, whole genome shotgun sequence.
ACCESSION   DATROQ010000229 DATROQ010000000
VERSION     DATROQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.243"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            14..84
                     /locus_tag="VBC94_002134"
     tRNA            14..84
                     /locus_tag="VBC94_002134"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Gly,seq:tcc)
     gene            119..192
                     /locus_tag="VBC94_002135"
     tRNA            119..192
                     /locus_tag="VBC94_002135"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:153..155,aa:Ile,seq:gat)
     gene            206..293
                     /locus_tag="VBC94_002136"
     tRNA            206..293
                     /locus_tag="VBC94_002136"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:240..242,aa:Ser,seq:gct)
BASE COUNT           81 a           60 c           80 g           93 t
ORIGIN      
        1 atataagttt ttagcgggtg tagtttagtg gtaaaactac agccttccaa gctgttgtcg
       61 cgagttcgat tctcgtcacc cgcttacttt acataaaagt aaaaccaagc atttgcttgg
      121 gcgcgtagct caggtggtta gagcgcacgc ctgataagcg tgaggtcggt ggttcgagtc
      181 cactcgtgcc cattaatatt aatttggaga attactcaag aggctgaaga ggacggtttg
      241 ctaaatcgtt aggtcgggta actggcgcaa gggttcgaat cccttattct ccgtaaatat
      301 tataatttta agag
//
LOCUS       DATROQ010000230          292 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.244, whole genome shotgun sequence.
ACCESSION   DATROQ010000230 DATROQ010000000
VERSION     DATROQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.244"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            21..>292
                     /locus_tag="VBC94_002137"
     CDS             21..>292
                     /locus_tag="VBC94_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063298.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEO6980344.1"
                     /translation="MNNNEKKVKYFLRKTAYGLASMSAAFIVCSGIVNTPTVSADSPD
                     TLKVEKLGKLKDVKSVHELTPISIPNELKGAKEQALSSIISHPNIT"
BASE COUNT          116 a           45 c           49 g           82 t
ORIGIN      
        1 aactatataa gggagcgaat atgaataata acgaaaaaaa agtaaaatac tttttaagaa
       61 aaacagctta tggtttggct tcaatgtcag cggcgtttat agtatgtagt ggtattgtaa
      121 atactcctac agtgtctgct gatagtcctg atacattaaa agtcgaaaaa ttaggcaaat
      181 tgaaagatgt gaaatcagtt catgaactca cacccatatc aataccgaac gaattaaaag
      241 gtgctaaaga gcaagcactt tcttcaataa tttcacatcc taatataact aa
//
LOCUS       DATROQ010000231          288 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.denovo.245, whole genome shotgun sequence.
ACCESSION   DATROQ010000231 DATROQ010000000
VERSION     DATROQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.denovo.245"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            complement(52..133)
                     /locus_tag="VBC94_002138"
     tRNA            complement(52..133)
                     /locus_tag="VBC94_002138"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(97..99),aa:Leu,seq:tag)
     gene            complement(143..215)
                     /locus_tag="VBC94_002139"
     tRNA            complement(143..215)
                     /locus_tag="VBC94_002139"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Lys,seq:ttt)
     gene            complement(218..288)
                     /locus_tag="VBC94_002140"
     tRNA            complement(218..288)
                     /locus_tag="VBC94_002140"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(255..257),aa:Asp,seq:gtc)
BASE COUNT           77 a           86 c           66 g           59 t
ORIGIN      
        1 tgattacaaa tcagatgctc taccaactga gctaagccgg caatctacta atgcgggtga
       61 agggacttga acccccacgc cgttaagcgc cagatcctaa atctggtgcg tctgccaatt
      121 ccgccacacc cgcatttcta aatgacccgt actgggctcg aaccagtgac ccattgatta
      181 aaagtcaatt gctctaccaa ctgagctaac gagtctacgg tcccgacggg aatcgaaccc
      241 gcgatcttcg ccgtgacagg gcgacgtgat aaccgctaca ctacggga
//
LOCUS       DATROQ010000232        14898 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699841-rid18553283.guided.044, whole genome shotgun sequence.
ACCESSION   DATROQ010000232 DATROQ010000000
VERSION     DATROQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699841
            Sequence Read Archive: SRR7789165
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790038.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790038.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:37:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,157
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 2,046
            CDSs (with protein)               :: 2,046
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 44 of 69
            Pseudo Genes (incomplete)         :: 12 of 69
            Pseudo Genes (internal stop)      :: 22 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14898
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699841-rid18553283.guided.044"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-11-03"
                     /collected_by="RMPRU"
     gene            7..195
                     /locus_tag="VBC94_002141"
     CDS             7..195
                     /locus_tag="VBC94_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, toxin component, MazF
                     family protein"
                     /protein_id="HEO6980345.1"
                     /translation="MSDIEVYSVLVSRIEYSDGTGSKVRPAVVVKFNDEVIKTLRFTT
                     KYENKSDNIRSQYLEVID"
     gene            348..719
                     /locus_tag="VBC94_002142"
     CDS             348..719
                     /locus_tag="VBC94_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEO6980346.1"
                     /translation="MKKKLLGLMILAISTVFLVACSTNSLNVKYYKVYDGEKMLVLEI
                     NDESGIYHNEGNFVVTNIDTKSKSFVLKGGSAKYTVLYDVKSDGKLNVDLGNFYYNAS
                     GKETYYKEGSKALEKALRDKE"
     gene            1090..1404
                     /locus_tag="VBC94_002143"
     CDS             1090..1404
                     /locus_tag="VBC94_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO6980347.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            1420..1806
                     /locus_tag="VBC94_002144"
     CDS             1420..1806
                     /locus_tag="VBC94_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO6980348.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1835..3220
                     /locus_tag="VBC94_002145"
     CDS             1835..3220
                     /locus_tag="VBC94_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6980349.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSMMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            3223..3375
                     /locus_tag="VBC94_002146"
     CDS             3223..3375
                     /locus_tag="VBC94_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6980350.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            3398..4603
                     /locus_tag="VBC94_002147"
     CDS             3398..4603
                     /locus_tag="VBC94_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO6980351.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            4646..4867
                     /locus_tag="VBC94_002148"
     CDS             4646..4867
                     /locus_tag="VBC94_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6980352.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            4984..5481
                     /locus_tag="VBC94_002149"
     CDS             4984..5481
                     /locus_tag="VBC94_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEO6980353.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            5456..5962
                     /locus_tag="VBC94_002150"
     CDS             5456..5962
                     /locus_tag="VBC94_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6980354.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            5946..8393
                     /locus_tag="VBC94_002151"
     CDS             5946..8393
                     /locus_tag="VBC94_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6980355.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            8396..10573
                     /locus_tag="VBC94_002152"
     CDS             8396..10573
                     /locus_tag="VBC94_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO6980356.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            10570..11451
                     /locus_tag="VBC94_002153"
     CDS             10570..11451
                     /locus_tag="VBC94_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEO6980357.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYTKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRT
                     AQQQYDVTQHIPLSEAQAGDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADL
                     TSPYWQQHLVGAGRIKQ"
     gene            11448..12380
                     /locus_tag="VBC94_002154"
     CDS             11448..12380
                     /locus_tag="VBC94_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6980358.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
     gene            12625..12741
                     /locus_tag="VBC94_002155"
     CDS             12625..12741
                     /locus_tag="VBC94_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO6980359.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            12757..14676
                     /gene="tet(M)"
                     /locus_tag="VBC94_002156"
     CDS             12757..14676
                     /gene="tet(M)"
                     /locus_tag="VBC94_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO6980360.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            14795..>14898
                     /locus_tag="VBC94_002157"
     CDS             14795..>14898
                     /locus_tag="VBC94_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO6980361.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENL"
BASE COUNT         4863 a         2627 c         3118 g         4290 t
ORIGIN      
        1 ttttaaatga gtgatattga ggtatattct gtattagttt ctcgtattga atattctgat
       61 ggtacaggtt caaaggttcg tcctgctgta gtagtcaaat ttaatgatga agtaataaaa
      121 actttaagat ttactactaa atatgaaaat aagtctgaca atataagaag tcagtattta
      181 gaagttattg attaggcaaa agcaaattta aataggcgtt cttggataga tactattcaa
      241 tattatgatg ttgagaataa agggttcaat attaaaatta taggtaagtt aagcgataga
      301 gatgttgaga ggttaaaaga ttttctcata gcaaaggaag tttagttatg aaaaagaaat
      361 tattaggatt gatgatttta gctatatcta cagtattttt agttgcttgt tcgactaatt
      421 cgttaaatgt aaagtattat aaagtatatg atggtgaaaa aatgttagtt ttagaaatta
      481 atgatgaatc aggaatttat cataatgaag gaaactttgt agtaactaat attgatacta
      541 aaagtaaaag ttttgtactt aaaggtgggt ctgctaagta tactgtttta tatgatgtta
      601 aaagtgatgg taaattgaat gttgatttag ggaactttta ttataatgct tctggtaagg
      661 agacatatta taaagaaggt agcaaggctt tagaaaaagc attaagagat aaagaataaa
      721 gttaggacta ttttcctaac ttatttttaa caaaaaaata gcataaaaat ctagttatcc
      781 gcataaaaac tggacttatc acactttatc aaggtcaaaa ccactcaatt tactactaat
      841 ttactactta tgaatgagct ttgatacgac gatttatcct tgaaaagtga agatataaag
      901 atacttccaa taaaatttga atatttaata ggtagacact tcaaaaaatg aggtgtctat
      961 ttttttaccc gattttgaaa ggaagtgaac ttatgaaaac aaaaaatcaa gaatcaaaag
     1021 gtcgttcccc actctttaag accatcaaac attcattcag ccaataaaaa agaaaggata
     1081 ggtaaaaata tggaacttaa atttgtgatt cccaacatgg aaaaaacatt cggcaattta
     1141 gaatttgctg gcgaggataa agtcgttcag cgaagaatca acggacggct aactgtctta
     1201 tcaagaagct ataatctcta ttctgatgtt caaagagcag atgatattgt ggtggtgctt
     1261 cctgctgaag ctggcgaaaa acatttcggc tttgaggaac gtgtgaagtt agtcaatcca
     1321 cgtattaccg cagagggcta caaaatcggc actcgtggtt ttacaaatta ccttttacat
     1381 gctgacgaca tgataaaaga ataaagaaag agaggaaaaa tgatgagatt agcaaatggc
     1441 attgtattag ataaagacac gacttttgga gaattgaaat tctctgctct acgtcgtgaa
     1501 gtgagaatcc aaaatgaaga cgggtcggtt tcagatgaaa tcaaggaacg tacctatgac
     1561 ttaaaatcca aaggacaagg acgcatgatt caagtaagta ttcctgccag cgtgcctttg
     1621 aaagagtttg attataacgc acgggtggaa cttatcaatc ccattgcgga caccgttgct
     1681 actgccacct atcaaggagc agatgttgac tggtatatca aggcagacga tattgtgctg
     1741 acaaaggatt ctagttcatt caaagctcaa ccacaagcaa agaaagaacc gacacaagac
     1801 aaatagtcgc taggtagaaa ggagactttt tcgcatgaaa cagcgtggta aaaggattcg
     1861 cccatctggt aaagatttag tctttcattt tacgatagcg tcactcctgc ctgttttcct
     1921 gctggttgtc ggactgtttc atgtgaagac aatccagcag atcaactggc aggattttaa
     1981 cctatcacaa gcagataaga ttgacattcc ctatttaatt atcagtttca gtgtcgcaat
     2041 tcttatctgc ttgctggtag cgtttgtatt caaacgggtt cgctatgata cggttaaaca
     2101 actttaccac cgtcaaaaac tggcaaagat gatacttgaa aacaagtggt atgaatctga
     2161 acaggtcaaa acagagggtt tctttaaaga tagtgctggt cgtacaaagg aaaagataac
     2221 ctacttccct aaaatgtatt atcgacttaa aaatggcttg atacagatac gggtggaaat
     2281 cacgctggga aaatatcaag accaactctt acacttggaa aagaaattag agagtggctt
     2341 gtactgtgag ctgacggata aagagttaaa ggattcctat gtggaatata ctttgctcta
     2401 tgacaccata gccagtcgta tttctattga tgaagtagaa gctaaagatg gtaaacttcg
     2461 cttaatgaaa aacgtatggt gggaatatga taagctccct catatgttga ttgctggtgg
     2521 tacaggtggc ggtaaaactt actttatact gacactgatt gaagccttgc ttcatacaga
     2581 ttcaaaactg tatattcttg acccgaaaaa tgctgatctt gcggacttag gttctatgat
     2641 ggcaaatgtc tactatagaa aagaagactt gctttcttgc attgaaacat tctatgaaga
     2701 aatgatgaaa cgtagtgagg aaatgaagca gatgaagaac tataagactg gcaaaaatta
     2761 tgcttactta ggtctcccgg cacacttctt aatctttgat gaatacgtcg ctttcatgga
     2821 aatgctggga acaaaagaaa acaccgcagt tatgaataag ctgaaacaga ttgtcatgtt
     2881 aggtcgtcaa gctggcttct ttctaatact ggcttgtcaa cgtccagacg caaaatattt
     2941 aggcgacgga atccgtgatc agtttaattt cagagtggct ttaggtcgta tgtctgaaat
     3001 gggctatggc atgatgtttg gcagtgacgt acaaaaggat ttcttcttaa agcgaatcaa
     3061 aggtcgtggc tatgttgatg taggaacaag tgtcatatca gagttttata ctccccttgt
     3121 accaaaagga tatgatttct tggaggaaat taaaaagtta tccaacagca gacagtccac
     3181 gcaggcgacg tgcgaagcgg aagtcgcagg tgtggactga tcttgctggc tggtgtggca
     3241 atagccacgc cagcacttaa ccccccgtat ctaacagggg ggtacaaatc gacaggaaac
     3301 agtcaaaaaa acattagaaa atcctttggt tacaagggat ttacaaaatt tcagcgtatg
     3361 tcaaatgggc tttaaaagtt gacatacgcc tttttgattg gagggatttt tactgaatga
     3421 acaaacttgg ttacagcatt taaaagaaaa acgcttggct tatggactat ctcaaaaccg
     3481 tttagctgtt gcgactggta ttacaaggca gtatctaagc gatattgaaa caggaaaagt
     3541 caagccatca gaggatttac agcagtccct ttgggaagct ctggaacgct tcaatcccga
     3601 cgctcccctt gaaatgctgt ttgattatgt aagaattcgc tttccgacaa cagacgtaca
     3661 gcaggtggtc gaaaacatct tacaactgaa actgtcctat tttcttcatg aggactatgg
     3721 tttctattct tattcagagc attatgcttt aggcgacata ttcgtccttt gctcccatga
     3781 actggacaaa ggagttctgg tggaattgaa aggtcgtggg tgcagacaat ttgaaagcta
     3841 tcttctggca caacaaagaa gctggtatga gttctttatg gacgttttgg tggctggcgg
     3901 tgtgatgaaa cgccttgacc ttgccattaa cgataagaca gggattttaa atatccctgt
     3961 actcactgaa aagtgccaac aggaagaatg tatctccgtc ttccgcagtt ttaaaagcta
     4021 tcgcagtggc gaactggtac gcaaagagga aaaggaatgt atgggaaaca ccctctatat
     4081 cggttcatta caaagtgaag tttatttctg tatctatgaa aaggactacg agcagtacaa
     4141 gaaaaatgat attcccattg aagacgcaga agtaaaaaac cgttttgaga ttcgattgaa
     4201 aaatgagcgt gcctattatg cagtccgtga tttactcgtc tatgacaatc cagagcatac
     4261 cgcctttaaa attatcaatc ggtatatccg ttttgtagat aaagacgatt ccaaacctcg
     4321 ttctgattgg aaactgaatg aagaatgggc ttggtttatt gggaacaatc gtgaacgatt
     4381 aaaactaacc acaaaaccag agccttactc cttccaaagg acgctgaact ggctatctca
     4441 tcaagttgcc ccgaccttaa aggttgcgat taaacttgat gaaatcaacc agacgcaggt
     4501 tgtaaaagac attctcgacc atgcgaaact gacagaccga cacaagcaga ttttgaagca
     4561 acagtcagta aaagaacagg acgtgataac aacaaaaaaa taactcaaat acaaattcat
     4621 tgaatataga gaggagaaca tttttatgaa ttttggacaa aacctttata actggtttct
     4681 atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt atcttggctt
     4741 caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg cggttggctt
     4801 agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc gcattattgg
     4861 tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt ccgcaaagga
     4921 atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa aggacgtgaa
     4981 tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga gggcgaattg
     5041 gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa aatcggcttg
     5101 aatgatgaat atgaggaata cgccattcat gactacgagt taccctttac ggttgacgaa
     5161 tacacttcca ttggcgaact caatcgacta tgggaaatgg tatcggaatt acccgaagaa
     5221 ttacaatcgg agctatctgc tctgctcact catttttcaa gcattgaaga actaagcgaa
     5281 catcaagagg atattatcat tcattccgat tgtgatgata tgtatgacgt ggcacgctac
     5341 tacattgaag aaacgggtgc tttaggcgaa gtaccagcta gtcttcaaaa ctatattgat
     5401 tatcaagcct atggtcggga tttagacctt tcaggaacgt ttatctcaac caatcatggg
     5461 atttttgaaa tcgtctatta aatctgtcgg tacattacta ctggcagatt ttctatttta
     5521 cggggtggct caatcagcta cccctatttt ttatgaaagg attgattaca tgaagaaaat
     5581 acgaagctat accagtatct ggtctgtgga aaaggtactg tattctatca atgattttag
     5641 acttccgttt cccataacct ttacgcaaat gacatggttt gtcgtgtcac tctttgcagt
     5701 gatgatactt ggcaacttgc cccctctttc catgatagag ggagcatttc tcaaatactt
     5761 tgggattcct gtggctttca catggtttat gtctacaaaa acttttgatg gtaaaaagcc
     5821 ttatggattt ttgaagtctg tcattgctta tgcactgcga ccaaagctga cctatgcagg
     5881 aaaaaaagta acgcttggca gaaaccagcc acaagaagcc attacagcag ttaggagtga
     5941 attttatggc atatccaatt aaatacattg aaaacaatct cgtctggaat aaagacgggg
     6001 aatgttatgc ttactatgag cttgttcctt acaattactc atttctaagt ccagaacaga
     6061 aaatacaagt gcatgattct ttcagacagc ttatcgcaca aaatcgtgat ggcaaaattc
     6121 atgctttaca aatcagtaca gaatccagca tacgttctgc acaagagcgt tccaaaaatg
     6181 aagtcactgg caagctcaaa gcggttgcct atgacaaaat cgaccaacag acagacgctt
     6241 taatatccat gattggcgaa aatcaagtga actaccgttt ctttatcggc tttaagttgc
     6301 ttctcaacga tcaggagttt tctatgaaaa gtcttaccgt tgaagcaaaa aatgctttgt
     6361 ctgattttgt ctatgatgtg aaccataagc tgatgggcga ttttgttagt atgagtaatg
     6421 atgaaatcct gcgttttcag aagatggaaa agctcttaga aaataaaatc tctcgtcgtt
     6481 tcaaaatccg caggttagat aaggacgact tcggctatct gattgaacac ctttacggac
     6541 agacaggcac tgcctatgaa gagtatgagt accatctatc aaagaaaaag ctggataatg
     6601 aaacgctgat taaatactat gacttgatta agcctactcg ctgtttggtg gaagaaaaac
     6661 agcgatattt gaaaatccag caggaagatg aaaccgtcta tgtagcttac tttaccatta
     6721 acagcattgt cggagaactg gacttcccgt cctctgaaat cttctactac cagcaacagc
     6781 aatttacatt cccgattgat acgtcaatga atgtggaaat tgtagcgaat cgtaaagccc
     6841 tatctactgt ccgcaataaa aagaaagaac tgaaagactt ggataaccac gcttggcaaa
     6901 gtgataatga aaccagctcc aatgtggcgg aagctctgga aagtgtgaat gagctggaaa
     6961 ccaatttaga ccaaagcaag gaatctatgt acaagctgtc ttatgtggta agggtatcag
     7021 caaatgatct tgacgaactc aaacgtcgtt gtaatgaagt gaaagatttt tatgacgatt
     7081 taagcgtaaa actggtacga ccatttgggg atatgctcgg cttacatgaa gaatttttac
     7141 ctgccagcaa gcgttatatg aatgattata ttcaatacgt gacctctgat ttcctcgctg
     7201 gtttaggttt tggtgctact caaatgctgg gggaaaatga ggggatttat gttggctaca
     7261 gcttagatac tggacgcaat gtctatctga aacctgctct tgccagtcaa ggggttaagg
     7321 gttcagtaac caatgcgtta gcgtcggctt ttgttggttc gctgggtggt ggtaaatcct
     7381 ttgcgaataa ccttatcgtc tattatgcgg tgctttatgg ggcacaagca gtgattgtag
     7441 acccaaaagc agaacgtggc agatggaaag aaaccttgcc agagatttcc catgaaatca
     7501 atatcgtcac tctgacttct gatgagaaaa acaaaggctt acttgaccct tatgtgatta
     7561 tgaaaaatcc caaagattct gaatcactgg ctattgatat tctgacattc cttacgggga
     7621 tttcctctcg tgatggggaa cgcttcccaa tccttagaaa agccattcgt gcagtaacca
     7681 atagtgaagt acgagggttg atgaaagtga ttgaggaatt acgggttgag aatacgccac
     7741 taagtaccag tatagccgac catatcgaaa gttttacaga ctatgacttt gcacatttat
     7801 tattcagtaa tggttatgtg gagcagtcta tcagcttaga aaaacaactg aacattatac
     7861 aggttgcgga cttggtactt cccgacaagg aaacttcctt tgaggaatat accactatgg
     7921 agcttttatc cgttgctatg ctgattgtca ttagtacctt tgctttagac tttatccata
     7981 cagaccgaag cattttcaag attgtagatt tagacgaagc atggagcttt ttacaggtag
     8041 cacaaggaaa aacactatct atgaagctgg ttcgggctgg tcgtgctatg aacgctgggg
     8101 tatatttcgt gacccaaaat acagacgacc tcttagatga aaaactgaaa aataacctcg
     8161 gcttaaaatt tgcatttcgt tccactgacc ttaacgagat taaaaagacc ttagcctttt
     8221 ttggtgtaga cccagaggac gaaaacaatc agaagcgatt gcgtgatttg gaaaacgggc
     8281 aatgccttat cagtgattta tatggtcgtg tcggtgtgat acagttccac cctgtatttg
     8341 aagaactgct ccatgccttt gataccagac cacctgtgcg aaaagaggtg taaatgtgaa
     8401 accatcaata gtaaacagaa taaaatcaaa ctggacgctg aaacgtctag gtaaagtggc
     8461 aatgacagtg gctttcacac ttgtgattgc catttttctt ttagccatgc tgggaacggt
     8521 ggttcaagct gcgggcttgg tagatgatac ggtcaatgtg gcaaatgaat acagccgata
     8581 cccacttgaa aactatcaac tggattttta tgtggataat agctggggct ggcttccgtg
     8641 gaactggtcg gacgggattg gaaaacaggt catgtatgga ctatatgcca ttaccaattt
     8701 tatttggaca atcagtttgt atgtttccaa tgcgacaggt tacttagtac aggaagccta
     8761 ttccttagac ttcatttccg ctacagcaga ttccattggt aagaatatgc agaccttagc
     8821 tggtgtgagt gcaaacggat tttcaacaga gggtttctat gttggattcc tcttactctt
     8881 gattttggtt cttggggttt atgttgccta tacgggactg ataaagagag aaaccacaaa
     8941 ggcaattcat gccattatga attttgtgct ggtgtttatc ctatcggctt cctttattgc
     9001 ctacgctccc gactacatta aaaaaatcaa tgacttttca tcagacatca gtaatgccag
     9061 tttatcactt ggcacgaaga ttgtcatgcc ccattccgat agtcaaggca aggacagcgt
     9121 ggacttaatc agagatagcc tgttttccat acaggttcag caaccgtggc tactgcttca
     9181 atacaacagt tcagacattg aaagtatcgg tattgaccgt gtggaaagcc tgctctccac
     9241 cagcccagat tccaacaatg gcgaagacag agaaaaaatt gttgcggaag aaattgaaga
     9301 cagaagcaat accaatctaa ccattacaaa gaccattaac cgtttaggta cagtcttctt
     9361 cctatttgtc ttcaatattg ggatttccat atttgtattc ctattaacag gaatcatgat
     9421 tttctcgcag gtacttttta tcatctatgc tatgtttctg cctgtgagct ttattttaag
     9481 catgattcca tcatttgatg gtatgtcaaa acgagccata acaaagctct ttaataccat
     9541 tttgacacga gctggaatca cattgattat tacgacagca tttagtattt caaccatgct
     9601 ctatacctta tcggctggtt atccgttctt tttgattgct tttctacaga ttgtgacctt
     9661 tgcaggaatc tacttcaagc tgggcgattt aatgagtatg ttttctctac agagtaacga
     9721 ttctcaaagt gtgggaagtc gtgtgatgag aaaacctcgt atgcttatgc acgctcacat
     9781 gcaccgtcta cagcggaaac ttggacgttc catgactact ctaggggctg ggtctgccat
     9841 tgttacaggt aaaaaaggac agtcgggttc ggggagttct gcaaggacac aagcagatca
     9901 ctcccgacca gacggaaagg aaaaatcaac acttggaaaa cgtatcggtc aaaccatcgg
     9961 tacagtagct gataccaaag acagaatggt agacactgct agtggtttga aagaacaggt
    10021 taaagatttg ccgaccaatg caagatatgc agtatatcaa ggaaaatcca aagtaaaaga
    10081 gaatgtccgt gatttaacca gtagtatttc tcaaaccaaa gcggacagag ccagtggacg
    10141 caaggaacag caggaacaaa ggcgaaaaac cattgcgaag cgtcgctctg aaatggaaca
    10201 ggtcaaacag aaaaaacagc ctgcttcttc tgttcatgaa agaccgacta caagacaaga
    10261 acaatatcat gatgaacaga cctcaaaaca gtctaatatt cagacttcat ataaggaatc
    10321 tcaacaagcc aaacaagagc gtccagcagt taagtccgat ttttcaagtc caaaagtgga
    10381 acgccaaggc aataccgttc aagaaaaaac cgttcaaaag ccagcaactt caaccactac
    10441 agcagataga acttcacaac gtccaatcac aaaagaacgt ccgtctactg ttcaaagagt
    10501 accactacaa aatacaagaa gtagaccacc aatcaaaacc gccaccatta agaaagtcgg
    10561 taagaaacca tgaagttgaa aactttagtg attggtggtt ctggattatt cttgatggtc
    10621 ttctcactgc ttctgtttgt tgccatttta ttttcagatg aacaggacag cggaatttcc
    10681 aatattcatt atggaggtgt gaatgtttcc gcagaagtgc tggctcataa gcctatggta
    10741 gaaaaatata ccaaagaata tggcgttgaa gaatatgtca acatacttct tgcgattata
    10801 caggtggaat cgggcggtac tgcggaagat gttatgcagt cctcggaatc cctcggtctt
    10861 ccacctaatt cattgagtac agaagaatcc attaagcaag gtgtgaagta tttcagtgaa
    10921 ttattagcca gtagcgaaag gctcagtgta gatttagaat cggttatcca gtcctacaat
    10981 tatggtggtg gtttcttagg gtatgtggct aatcgtggaa ataaatatac ctttgaactg
    11041 gctcaaagtt tctcaaaaga gtattcaggt ggcgaaaaag tgtcttaccc caatcccata
    11101 gccataccta tcaatggggg ctggcgatac gtttacggtg gagcttcccc gactacttct
    11161 tttgattgta gcggactgac acaatggacg tatggaaaag ctggaattaa cttaccacga
    11221 accgcacaac agcaatatga tgtgacccag catatcccac tatcggaagc acaagctggc
    11281 gatttggttt tctttcattc tacctataac gctggctctt atattactca tgttgggata
    11341 taccttggca ataaccgtat gtttcatgca ggcgacccaa tcggttatgc cgacttaaca
    11401 agcccctact ggcaacagca tttagtggga gcaggacgaa tcaaacaatg agaaaggaag
    11461 atttaatgat gaaatttaga aaaaatcaga ataaagaaaa acagatacca aaggaaaaga
    11521 aacctcgtgt ctataaggtc aatcctcata aaaaggttgt gattgccttg tgggtacttt
    11581 tagggcttag tttcagcttt gcgatattca agcactttac agctatagat actcatacta
    11641 ttcacgaaac aactatcata gaaaaggaat acgttgatac tcatcatgta gaaaattttg
    11701 tagagaactt tgcgaaagtc tactattcat gggagcaatc cgataagtcc attgataatc
    11761 gaatggaaag tctaaaaggc tatctgacag atgaacttca agctctcaat gttgatacag
    11821 tacgcaaaga tattcctgta tcgtcttctg taagaggatt tcagatatgg acggtagagc
    11881 caactggcga caatgagttt aatgtaacct acagtgtaga ccagctcatt acagagggag
    11941 aaaatacaaa gaccgtccac tctgcttata tagtgagtgt ctatgtagat ggttctggaa
    12001 atatggtact ggttaagaat ccgaccatta ccaacatacc taagaaatca agttataaac
    12061 caaaagccat tgaaagtgag gggacggttg attccattac aaccaatgaa atcaatgagt
    12121 ttttaacgac gttcttcaag ctctatccta cagcgacagc cagtgaactt tcctactatg
    12181 tgaatgacgg gatattaaaa ccaatcggaa aagagtacat ctttcaagaa ctggtaaatc
    12241 ctattcacaa tcgtaaggat aatcaagtca cggtatcgct gacagtggag tatatcgacc
    12301 agcagaccaa agcaacgcag gtatctcaat ttgatttggt acttgaaaag aacgggagta
    12361 attggaagat tgtaaaataa caaatattgg tacatgatta cagatacttt gtaatcatgt
    12421 actctttttg ataaaaaatt ggagattcct ttacaaatat gctcttacgt gctattattt
    12481 aagtgactat ttaaaaggag ttaataaata tgcggcaagg tattcttaaa taaactgtca
    12541 atttgatagc gggaacaaat aattagatgt ccttttttag gagggcttag ttttttgtac
    12601 ccagtttaag aataccttta tcatgtgatt ctaaagtatc cagagaatat ctgtatgctt
    12661 tgtataccta tggttatgca taaaaatccc agtgataaaa gtatttatca ctgggatttt
    12721 tatgcccttt tgggtttttg aatggaggaa aatcacatga aaattattaa tattggagtt
    12781 ttagctcatg ttgatgcagg aaaaactacc ttaacagaaa gcttattata taacagtgga
    12841 gcgattacag aattaggaag cgtggacaaa ggtacaacga ggacggataa tacgctttta
    12901 gaacgtcaga gaggaattac aattcagaca ggaataacct cttttcagtg ggaaaatacg
    12961 aaggtgaaca tcatagacac gccaggacat atggatttct tagcagaagt atatcgttca
    13021 ttatcagttt tagatggggc aattctactg atttctgcaa aagatggcgt acaagcacaa
    13081 actcgtatat tatttcatgc acttaggaaa atggggattc ccacaatctt ttttatcaat
    13141 aagattgacc aaaatggaat tgatttatca acggtttatc aggatattaa agagaaactt
    13201 tctgccgaaa ttgtaatcaa acagaaggta gaactgtatc ctaatatgtg tgtgacgaac
    13261 tttaccgaat ctgaacaatg ggatacggta atagagggaa acgatgacct tttagagaaa
    13321 tatatgtccg gtaaatcatt agaagcattg gaactcgaac aagaggaaag cataagattt
    13381 cataattgtt ccctgttccc tgtttatcac ggaagtgcaa aaaacaatat agggattgat
    13441 aaccttatag aagtgattac gaataaattt tattcatcaa cacatcgagg tccgtctgaa
    13501 ctttgcggaa atgttttcaa aattgaatat acaaaaaaaa gacaacgtct tgcatatata
    13561 cgcctttata gtggagtact acatttacga gattcggtta gagtatcaga aaaagaaaaa
    13621 ataaaagtta cagaaatgta tacttcaata aatggtgaat tatgtaagat tgatagagct
    13681 tattctggag aaattgttat tttgcaaaat gagtttttga agttaaatag tgttcttgga
    13741 gatacaaaac tattgccaca gagaaaaaag attgaaaatc cgcaccctct actacaaaca
    13801 actgttgaac cgagtaaacc tgaacagaga gaaatgttgc ttgatgccct tttggaaatc
    13861 tcagatagtg atccgcttct acgatattac gtggattcta cgacacatga aattatactt
    13921 tctttcttag ggaaagtaca aatggaagtg attagtgcac tgttgcaaga aaagtatcat
    13981 gtggagatag aactaaaaga gcctacagtc atttatatgg agagaccgtt aaaaaatgca
    14041 gaatatacca ttcacatcga agtgccgcca aatcctttct gggcttccat tggtttatct
    14101 gtatcaccgc ttccgttggg aagtggaatg cagtatgaga gctcggtttc tcttggatac
    14161 ttaaatcaat catttcaaaa tgcagttatg gaagggatac gctatggttg cgaacaagga
    14221 ttatatggtt ggaatgtgac ggattgtaaa atctgtttta agtatggctt atactatagc
    14281 cctgttagta ccccagcaga ttttcggatg cttgctccta ttgtattgga acaagtctta
    14341 aaaaaagctg gaacagaatt gttagagcca tatcttagtt ttaaaattta tgcgccacag
    14401 gaatatcttt cacgagcata caacgatgct cctaaatatt gtgcgaacat cgtagacact
    14461 caattgaaaa ataatgaggt cattcttagt ggagaaatcc ctgctcggtg tattcaagaa
    14521 tatcgtagtg atttaacttt ctttacaaat ggacgtagtg tttgtttaac agagttaaaa
    14581 gggtaccatg ttactaccgg tgaacctgtt tgccagcccc gtcgtccaaa tagtcggata
    14641 gataaagtac gatatatgtt caataaaata acttagtgta ttttatgttg ttatataaat
    14701 atggtttctt gttaaataag atgaaatatt ttttaataaa gatttgaatt aaagtgtaaa
    14761 ggaggagata gttattataa actacaagtg gatattgtgt cctgtatgtg gaaataaaac
    14821 acgattaaag ataagggaag atactgaatt aaaaaaattc cccctctatt gtccgaaatg
    14881 cagacaagaa aatttaat
//
